LOCUS       DATABV010000001         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2088551, whole genome shotgun sequence.
ACCESSION   DATABV010000001 DATABV010000000
VERSION     DATABV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088551"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="ftsL"
                     /locus_tag="VHF88_00005"
     CDS             <1..594
                     /gene="ftsL"
                     /locus_tag="VHF88_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX2160188.1"
                     /translation="QRTGALARSRAVAGGEALLHGPGLIAIVFVLLAGVVFANVALLQ
                     KNRQITHDAARVSELKRDNATLRREVAELGSSERIQQVAAERGLVLPAPDAVRYLRSN
                     PRSDAVAAAKRIDESTALAAVAEMTPTYDPVAATPTYTAPVDPATAAYGAAPTYDAYG
                     NPVTASAPTYDAYGNPTTATEIAATPDAVSDVTAGVP"
     gene            604..2247
                     /locus_tag="VHF88_00010"
     CDS             604..2247
                     /locus_tag="VHF88_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2160189.1"
                     /translation="MRLVDRRIGLIFALFLLLLGAATARAAWIGTVGSGDLKARAATQ
                     QVEELTVSARRGTITDRNGVELAVSEDSVTVFGNPLQIKDPAGVAAKLAPIVDESAQE
                     LTELLADRSTGFVYLARKIPPAAGEKVEKLEIEGIGTVSEPRRTYPQGELAGQLIGAV
                     GTDNFGLTGIEQQYEETLHGDDGERKIVRDALGEPVSIVDTDSSEPGEDLQLTIDAAL
                     QERVEAVMEEVATTFAPKGATAVVMDPRNGEVLSLANWPRVDPNDIGAAEEDARENRA
                     VMSAFEPGSTFKAFTVAGALEEGVITPETRFDLPPELQVADRTIGEAHARGYVNLSVG
                     EILAQSSNVGSVMIGQKLGPKRFDKWVRKFGFGSLTGVDLPGESPGIVLRPREYSGSS
                     IGNLPIGQGIAVTPLQMATGFSAIANGGVLHEPTVVAGEADEGRRVVSERTADQVAEM
                     LEGVLAAGGTAEEASIPGYVVAGKTGTAEKVDPETGEYSETKFFSSFIGFAPAEDPRL
                     MVAVMVDEPSGMYYGGEVAAPAFEKIMEFALPYLRIPPD"
     gene            complement(2398..>3442)
                     /locus_tag="VHF88_00015"
     CDS             complement(2398..>3442)
                     /locus_tag="VHF88_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX2160190.1"
                     /translation="LRAALAEMPDSDLGYGDPCGLTELRSEIAAYLRRVRAVDADARR
                     TLITSGTVQAIDLACRALRSRGACRVAVEDPGWAAQAATVQRAGLEPVPVPVDDAGID
                     VDHLDGLDVDAALVTPAHQFPTGAVLSPPRRHRLVEWAAQRNALIVEDDYDAEYRYDR
                     NATGSLQGLAPDHVAYAGSASKTLAPALRLGWLVLPPSLVNATTAEKRDADRMTSPLT
                     QLAFARFLARGDLDRHLRQTRLLYRDRRAALLEALAHHVPDAELSGAAAGLHAVMLLH
                     PTADEPRIVSDARSAGIALTGLADLHLTARPHRPGLVLGYAQLSPDRIATGIAALAKV
                     VANAQAAPPLSHP"
BASE COUNT          501 a         1108 c         1366 g          467 t
ORIGIN      
        1 cagcgcacag gggcgctcgc acgctcgcgc gccgtagccg gcggcgaggc gctgctgcac
       61 ggacctgggc tgatcgcgat cgtcttcgtg ctgctggccg gcgtcgtctt cgccaacgtc
      121 gcactgctgc agaagaaccg ccagatcacc cacgacgcgg cgcgcgtgtc cgagctcaag
      181 cgcgacaacg ccacgctccg gcgcgaggta gccgagctcg gctcgagcga gcggatccag
      241 caggtggcag ccgagcgcgg cctcgtgctg ccggcgcccg acgcagtccg ctacctgcgc
      301 tcgaacccgc gctcggacgc ggtcgcggcc gccaagcgga tcgacgagag cacggccctg
      361 gcagcggtcg cggagatgac gccgacttac gaccccgtcg cggcgacgcc gacgtacacg
      421 gcgccggtcg atccggcgac cgccgcctac ggagccgctc cgacatacga cgcctacggc
      481 aatccggtca ccgcgtccgc cccgacctat gacgcctacg gcaacccgac gaccgcaacc
      541 gagatcgcag ccacccccga cgccgtcagc gacgtgacgg ccggcgttcc ctaggagctg
      601 tcggtgcggc tagtcgaccg ccggatcggc ctgatcttcg ccctcttcct actcctcctc
      661 ggcgcagcca ccgcgcgtgc cgcctggatc ggcaccgtcg gatcgggaga cctcaaggcg
      721 cgcgccgcga cgcagcaggt cgaggagctc accgtgtcgg cgcgccgcgg cacgatcacc
      781 gaccgcaacg gcgtcgagct cgcggtgtcc gaggactcgg tgaccgtctt cgggaacccc
      841 ctccagatca aggatcccgc cggcgtcgcc gcgaagctgg cgccgatcgt cgacgagtcc
      901 gcacaggagc tgaccgagct gctcgccgac cgcagcaccg gcttcgtata cctcgcgcgc
      961 aagatcccac cggccgccgg cgagaaggtc gagaagctcg agatcgaggg catcgggacc
     1021 gtctccgagc cgcgccggac ctatccgcag ggcgagctcg cgggccagct gatcggcgcg
     1081 gtcgggaccg acaacttcgg cctcaccggc atcgagcagc agtacgagga gacgctgcac
     1141 ggcgacgacg gcgagcgcaa gatcgtccgc gacgcgctcg gcgagccggt ctcgatcgtc
     1201 gacaccgaca gctccgagcc gggcgaggac ctccagctga cgatcgacgc cgcgctgcag
     1261 gagcgcgtcg aggccgtgat ggaggaggta gccacgacct tcgcgcccaa gggcgctacc
     1321 gccgtcgtca tggacccgcg caacggggag gtgctgtcgc tcgcgaactg gccgcgcgtc
     1381 gaccccaacg acatcggcgc cgccgaagag gatgcgcgcg agaaccgcgc ggtgatgtcg
     1441 gccttcgagc ccggctcgac cttcaaggcc ttcacggtcg ccggggcgct cgaggagggg
     1501 gtgatcacgc ccgagacgcg gttcgacctg ccgcccgagc tgcaggtcgc cgaccggacg
     1561 atcggcgagg cgcacgcgcg cggctacgtc aacctgtcgg tcggcgagat cctcgcgcag
     1621 tcgtcgaacg tcgggtcggt gatgatcggc cagaagctcg ggccgaagcg cttcgacaag
     1681 tgggtgcgga agttcgggtt cggctcgctg accggcgtcg acctgccggg cgagtcgccc
     1741 gggatcgtcc tgcgcccgcg cgagtactcg gggtcgtcga tcggcaacct gccgatcggg
     1801 cagggcatcg ccgtgacgcc gctgcagatg gcgaccggct tctcggcgat cgccaacggc
     1861 ggcgtgctgc acgagccgac ggtggtcgcg ggggaggccg acgagggacg gcgggtcgtg
     1921 tcggagagga ccgcggacca ggtggcggag atgctcgagg gcgtgctggc cgctggcggg
     1981 acggcggagg aggcgtcgat tcccgggtat gtggtcgcgg ggaagacggg aacggcggag
     2041 aaggtcgatc ccgagacggg ggagtactcc gagacgaagt tcttctcgtc gttcatcggg
     2101 tttgcgccgg cggaggatcc gcggttgatg gtggcggtga tggtggacga gccgtcgggg
     2161 atgtactacg gcggcgaggt ggcggcgccg gcgttcgaga agatcatgga gttcgcgctg
     2221 ccgtatctgc ggattccgcc tgattgaggg ttctacggcc gagggacgga ccgtgcaggc
     2281 cccgggtcct gtctcttgga gtgcaccgct cgcaagctcg cgttgtgatc cgggagagtc
     2341 cgtgctcgca agctgcgcgc gcaaacgcaa aatcgtcgcc acccggggga gggacgtcta
     2401 cggatgcgag agcggagggg ctgcctgtgc gttggcgacg actttggcga gcgctgctat
     2461 tccggtcgcg atccggtccg gggagagctg ggcgtagcca aggactaggc cggggcggtg
     2521 tggacgggcg gtgaggtgga ggtcggctag gccggtgagg gctatgccgg ctgagcgggc
     2581 gtcggagacg atgcggggct cgtcggcggt cgggtggagg agcatgacgg cgtggaggcc
     2641 ggccgcggct ccagagagct cggcgtctgg gacgtggtgg gcgagggcct cgagcagggc
     2701 ggcgcggcgg tcgcggtaga ggagacgggt ctggcgtagg tggcggtcga ggtcgccgcg
     2761 ggcgaggaag cgggcgaagg cgagctgggt gaggggggag gtcatgcggt cggcgtcgcg
     2821 cttctcggcg gtcgttgcgt tgacgaggct gggtgggagg accagccagc cgaggcggag
     2881 ggcgggggcg agggtcttgc tggcggagcc ggcgtaggcg acatggtcgg gcgcgagtcc
     2941 ctgaaggctg ccggtggcat tgcggtcgta gcggtactcg gcgtcgtagt cgtcctcgac
     3001 gatgagggcg ttgcgctgcg cggcccactc gacgagccgg tggcggcgcg gcgggctgag
     3061 cacggcgccg gtcgggaact ggtgcgccgg ggtgacgagg gcggcgtcga cgtcgaggcc
     3121 gtcgaggtgg tcgacgtcga tgccggcgtc gtcgactggg acgggcaccg gctcgaggcc
     3181 ggctcgctgg accgtggcgg cctgcgcggc ccagccgggg tcctcgacgg cgacgcgaca
     3241 cgctccgcgg ctgcggaggg cccggcaggc caggtcgatg gcctggaccg tgccgctggt
     3301 gatcagcgtg cggcgcgcgt cggcgtcgac cgcccggacg cggcggaggt aggcggcgat
     3361 ctcggagcgg agctcggtca ggccgcaagg gtctccatag ccgaggtcgg agtccggcat
     3421 ctcggccagt gcggcgcgca gc
//
LOCUS       DATABV010000002         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1526358, whole genome shotgun sequence.
ACCESSION   DATABV010000002 DATABV010000000
VERSION     DATABV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526358"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="mraW"
                     /locus_tag="VHF88_00020"
     CDS             complement(<1..514)
                     /gene="mraW"
                     /locus_tag="VHF88_00020"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX2160191.1"
                     /translation="MTRAHVPVLAGELIELADPAAGEQVVDCTFGAGGHARLIADRIG
                     PAGTLVCVDRDPAAEARFEELAAEVPCETRFLRMDYADALELLRAEGFAADLVYMDLG
                     VSSMQVDTRERGFSYSYDAPLDMRMDPTQELDARTIVNEWDERQLARLFRRYGEDPGA
                     QSTAREIARRR"
     gene            complement(516..950)
                     /gene="mraZ"
                     /locus_tag="VHF88_00025"
     CDS             complement(516..950)
                     /gene="mraZ"
                     /locus_tag="VHF88_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX2160192.1"
                     /translation="MAFRGHYEHSLDAKNRLSIPARFRATFSSGLVLAKDSDECISVW
                     TPEAHESIIERALAGKNPLGREYRAVQRYFQANSFDVELDSSGRVTLPPPLLAHAGIE
                     KETVVAGVGDHLEVWGRKRWDDEQADLDASIGEVTEGLGDPS"
     gene            1317..2219
                     /locus_tag="VHF88_00030"
     CDS             1317..2219
                     /locus_tag="VHF88_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2160193.1"
                     /translation="MSTASPALRLRGVVKRYGPITAVNGLDLEVPPGTCVGLLGPNGA
                     GKSTTMRLLTAQAIADEGTIEVLGYELPRESKQARAECGVVPQLDNLDTTLTVEQNLI
                     VFSHLYRVPRRDRPAAIERALELAKLTDRRDTVVDELSGGMRRRLLIARGLVHQPRLV
                     LLDEPTVGLDPQVRQELWALIDRLRSEGISILMSTHYIEEAERLADSVTIMSHGSVVA
                     SGRPTDLVAEHAGGEVLEVYGPPTRLAEIEAQAAAAGRRTRRTGTSVTVIGDMSANGS
                     GLEGVRRAGNLEDVFVLLTGEEIA"
     gene            2216..>2774
                     /locus_tag="VHF88_00035"
     CDS             2216..>2774
                     /locus_tag="VHF88_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160194.1"
                     /translation="MTGAAVTRDGPAAPPPRPPTRKLHRFEGSAIGGVLVREMINFSS
                     YWRSTTFSSTVEPTIYLLAFGFGLGSLVSSVGNGISYEEFVGTGMVAMAVLFASAMPS
                     MFGTFVKYQFQHTYDAILAAPVDTEELVTAEALWMAMRASVYGCAPLIVAMAFGLDPS
                     WGMLAVPAINFIAGFGWSCFGIAIAA"
BASE COUNT          399 a          982 c          969 g          424 t
ORIGIN      
        1 gccggcggcg agcgatctcg cgcgcggtcg actgcgcccc gggatcctcg ccgtagcggc
       61 ggaagagccg cgcgagctgg cgctcgtccc actcgttgac gatcgtgcgg gcgtcgagct
      121 cctgggtcgg gtccatccgc atgtcgagcg gagcgtcgta ggagtacgag aagccgcgct
      181 cgcgcgtgtc gacctgcatc gacgacacgc cgaggtccat gtagacgagg tctgccgcga
      241 agccctcggc gcgcagcagc tcgagcgcgt cggcgtagtc catgcgcagg aagcgggtct
      301 cgcaggggac ctcggccgcc agctcctcga agcgcgcctc ggctgccggg tcgcggtcga
      361 cgcagacgag cgtgccggcg ggaccgatcc ggtcggcgat cagccgcgcg tggccgccgg
      421 cgccgaacgt gcagtcgacg acctgctcgc ccgccgccgg gtcggccagc tcgatcagct
      481 cgccggccag gacgggtacg tgcgcgcgcg tcatgtcaag aaggatcgcc aaggccctcg
      541 gtcacctccc cgatgctcgc gtcgaggtcc gcctgctcgt cgtcccagcg cttgcggccc
      601 cagacctcca ggtggtcccc cactccggcg acgacggtct ccttctcgat ccccgcgtgc
      661 gcgagcagcg gcggcggcag tgtgacgcgg ccggaggagt cgagctcgac gtcgaaggag
      721 ttcgcctgga agtagcgctg cacggcacgg tactcgcggc cgagcgggtt cttgccggcg
      781 agcgcgcgct cgatgatcga ctcgtgggcc tccggggtcc agacggagat gcactcgtcg
      841 ctgtccttgg cgagcacgag tccggaggag aaggtcgcgc ggaagcgggc cggaatgctg
      901 agccggttct tggcatcgag cgaatgttcg tagtggcctc tgaatgccat ccggagctcc
      961 gtggaagtga gctcgacgcg gaccgaagtg gctgagtggg aggagcccag ccactccggt
     1021 ccttgtcgat gcgcgccact gtatcccact ctctcccaca ctgcaacaca tccacccact
     1081 tctagccaag tcgactccga actggagagc tattgcgccc gaagttccgc attgaaactg
     1141 gaaatccttg tggtggcgga cggtgggacg ccctcccgcg attaccggcc gagaccagcc
     1201 aaatctcgcg catgggtggg aaatcccatg cggcagcgct ggtgcaacgg cgattgcagc
     1261 ccgcgctcgg ttcgcggccc ggcgaccgac cacgcctgtc cgtaccatcg tgcgccgtgt
     1321 cgaccgcctc gccagccctg cgcctacgcg gcgtcgtcaa gcgctatgga ccgatcaccg
     1381 cggtgaacgg gctcgatctc gaagtcccac caggcacctg cgtcggcctg ctcggtccca
     1441 acggcgccgg caagtcgacg acgatgcgcc tgctgaccgc gcaggcgatc gccgacgagg
     1501 ggacgatcga ggtgctcggc tacgagctcc cgcgcgagtc gaagcaggcg cgcgccgagt
     1561 gcggggtcgt cccccagctc gacaacctcg acaccacgct gaccgtcgag cagaacctga
     1621 tcgtcttcag ccacctctac cgggtgccgc gccgcgaccg ccccgcggcg atcgagcgcg
     1681 cgctcgagct ggcgaagctg accgaccgcc gcgacacggt ggtcgatgag ctgtcgggcg
     1741 ggatgcgccg ccggctgctg atcgcgcgcg gcctggtcca ccagccgcgg ctggtgctgc
     1801 tcgacgagcc gaccgtcggg ctcgacccgc aggtgcgcca ggagctgtgg gcgctgatcg
     1861 accggctgcg ttccgagggc atctcgatcc tgatgtcgac ccactacatc gaggaggccg
     1921 agcggctcgc cgacagcgtg acgatcatgt cgcacggcag cgtggtggcg tccgggaggc
     1981 cgaccgacct ggtcgccgag cacgccggcg gcgaggtgct cgaggtctac gggccgccga
     2041 cgcggctcgc cgagatcgag gcgcaggccg cggcggcggg acggcgcacg cgccggaccg
     2101 ggacctccgt gaccgtgatc ggcgacatgt cggcgaacgg cagcggcctc gagggggtcc
     2161 gtcgtgccgg caacctcgag gacgtcttcg tgctgctgac cggcgaggag atcgcgtgac
     2221 cggcgccgcg gtcacccggg acggccccgc cgcgccgccc ccacgcccgc cgacgcgcaa
     2281 gctgcaccgc ttcgagggct cggcgatcgg cggcgtgctc gtgcgcgaga tgatcaactt
     2341 ctcgtcgtac tggcgctcga cgaccttctc ctcgacggtc gagccgacga tctacctgct
     2401 cgccttcggc ttcggcctgg gctcgctcgt ctcgagcgtc ggcaacggga tctcctacga
     2461 ggagttcgtc ggcaccggca tggtcgcgat ggctgtgctg ttcgcgtcgg cgatgccgtc
     2521 gatgttcggc accttcgtca agtaccagtt ccagcacacc tacgacgcga tcctcgccgc
     2581 ccccgtcgac accgaggagc tcgtcaccgc cgaggcgctc tggatggcga tgcgcgcctc
     2641 ggtctacggc tgcgcgccgc tgatcgtcgc gatggcgttc gggctagacc ccagctgggg
     2701 catgctcgcg gtccccgcga tcaacttcat cgccggcttc ggctggtcgt gcttcggcat
     2761 cgcgatcgcc gcct
//
LOCUS       DATABV010000003         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1929229, whole genome shotgun sequence.
ACCESSION   DATABV010000003 DATABV010000000
VERSION     DATABV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929229"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VHF88_00040"
     CDS             <1..1423
                     /locus_tag="VHF88_00040"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEX2160195.1"
                     /translation="IERYGPVDTVIGTNGSGAALQQYNAANNAPGLISAAMPTASFAD
                     ILTTAMTVTDCGLLQRYYAESNLKWTDAKKAAVNGHNLLSGNRLNAICQSWTDAFLEN
                     IRPDTDCVPEDVVYDPETNPDGVRCTLQDSNVNVFGVDPETGFARRPLDNVGVQYGLR
                     ALRRGVISMREFIDLNRKIGGYDIDGRHQAKRHEMDREVEALAYRLGGVIGRGALAET
                     PVLDFGAYLDLIPVANIHESVRPFVIRARLRKEAGNHRSQAIWRGIATQPDGFDAMDE
                     WLRNLERRRPAPGGDHAAAVRLARPTSAADRCTFGTVGGRLELPDAIKLPLGLAQLPL
                     LTNVPLLHDLLRDLFGDVDLNVPIRVDVYENFGNGYTGRGICSLALPVVRTPRMIAGM
                     PLADDVIKCRLKPVTRVGYPGVTAAQLAQIRAIFPDGVCDYSKDAAGDVERSMIWPSL
                     GGDQLQAPHELRWRVARSEAETG"
     gene            1700..>2266
                     /locus_tag="VHF88_00045"
     CDS             1700..>2266
                     /locus_tag="VHF88_00045"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR00034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX2160196.1"
                     /translation="MNFEMKELPRHHSDRVRDRRIERAVELVSPAQILEDLPLGAERE
                     EAVLRHRADVASILNREDDRLLVIVGPCSVHDVDAALDYASRLSARAQELGDELCIAM
                     RVYFEKPRTTVGWKGLINDPHLDGSRDVNTGLHMARSLLLEVLDLGLPIGCEFLDPIT
                     PQYISDVVAWGAIGARTTESQIHRQLASG"
BASE COUNT          370 a          825 c          758 g          313 t
ORIGIN      
        1 catcgagcgc tacgggccgg tagacaccgt gatcggcacg aacggctccg gcgccgcgct
       61 gcagcagtac aacgccgcca acaacgcgcc gggcctgatc tcggcggcga tgccgaccgc
      121 gagcttcgcc gacatcctga ccacggcgat gacggtgacc gactgcggcc tgctccagcg
      181 ctactacgcc gaatcgaacc tgaagtggac cgacgcgaag aaggcggccg tcaacggcca
      241 caacctgctc tcgggcaacc ggctgaacgc gatctgccag tcgtggacgg acgctttcct
      301 cgagaacatc cgccctgaca ccgactgcgt tcccgaggac gtcgtctacg acccggagac
      361 gaatcccgac ggggtccgct gcacgctcca ggactcgaac gtgaacgtct tcggcgtcga
      421 tcccgagacg gggttcgcgc gccgcccgct cgacaacgtc ggtgtccagt acgggctgcg
      481 agccctgcgg cggggggtga tctcgatgcg cgagttcatc gacctcaacc ggaagatcgg
      541 cggctacgac atcgacggtc gccaccaggc gaagcggcac gagatggacc gcgaggtcga
      601 ggcgctcgcc taccggctcg gcggagtgat cggccggggc gccctcgcgg agaccccggt
      661 gctcgacttc ggcgcctacc tcgacctgat tccggtcgcg aacattcacg agtcggtgcg
      721 tccgttcgtg atccgcgccc gactccggaa ggaagccggc aaccaccgct cgcaggcgat
      781 ctggcgcggg atcgcgacgc agccggacgg gttcgatgcg atggacgagt ggctgcggaa
      841 cctcgagcgc aggcgccccg ccccgggcgg cgatcacgcc gcggcggttc ggctggcgcg
      901 gccaacgtcg gccgccgacc gctgcacctt cggaactgtc ggcgggcggc tcgagctccc
      961 cgacgcgatc aagctgccgc tcggcctcgc gcagctgccg ctgctgacca acgtgccgct
     1021 cctgcacgac ctcctccgcg atctcttcgg cgacgtcgac ctgaacgtcc cgatccgggt
     1081 cgacgtctac gagaacttcg gcaacggcta taccggccgc ggcatctgct cacttgcgct
     1141 ccccgtcgtc cgcaccccgc ggatgatcgc ggggatgccg ctcgccgacg acgtgatcaa
     1201 gtgccggctg aagccggtga ccagggtggg ctaccccggc gtcaccgccg ctcagctcgc
     1261 gcagatccgc gcgatcttcc ccgacggggt ctgcgactac tcgaaggacg ccgccggcga
     1321 cgtcgagcgg agcatgatct ggccctcgct cggcggggac cagctgcagg cgccgcacga
     1381 gctccgttgg cgtgtggcgc gctcggaggc cgagacgggt tagccggcgt cggcatcggc
     1441 gctgagttcg acaatcgcag gcgcgtctgg cagaatcgga cgccgaatga acgcaggcac
     1501 gccgatcgca gcctcgccga gccgccacgc ggctccttcg cgtgcctatt tctacttctg
     1561 gcgatttagc taggcgtccg gcccgcggct gccccaagcc gcacacgccg aagcaacacc
     1621 accgccgggc gccgagaccc aaccgcagcg cgcgccgctg cgaggacctt cgccagaccg
     1681 ctagaaagag gaaccaggaa tgaacttcga gatgaaggag ctaccgagac atcacagcga
     1741 ccgcgtccgc gaccggcgga tcgagcgcgc ggtcgagctc gtcagccccg cgcagatcct
     1801 cgaggacctg ccgctgggcg ccgagcgtga ggaggcggtg ctgcgtcacc gcgccgacgt
     1861 cgcctcgatc ctgaaccgcg aggacgaccg cctgctcgtg atcgtcggcc cgtgcagcgt
     1921 ccacgacgtc gacgccgcgc tcgactacgc ctcgcggctg agcgcccgcg cgcaggagct
     1981 cggcgacgag ctctgcatcg cgatgcgcgt ctacttcgag aagccgagga ccaccgtcgg
     2041 ctggaagggg ctgatcaacg acccgcacct ggacggctcg cgcgacgtca acaccggcct
     2101 ccacatggcg cgctcgctgc tgctcgaggt gctcgacctc gggctcccga tcgggtgcga
     2161 gttcctcgac ccgatcacgc cgcagtacat ctccgacgtc gtcgcctggg gggcgatcgg
     2221 ggcgcgcacg accgagagcc agatccaccg ccagctcgcg tcgggg
//
LOCUS       DATABV010000004         6982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_403521, whole genome shotgun sequence.
ACCESSION   DATABV010000004 DATABV010000000
VERSION     DATABV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403521"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VHF88_00050"
     CDS             <1..1053
                     /locus_tag="VHF88_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX2160197.1"
                     /translation="LGVRGFLPASLVDIRRVQDLDEFMGQKIECKVIELNRSRNNVVL
                     SRRAVLEEERKEVRQQILDRLQPGQVVEGSISNIVDCGAFVDLDGIDGLIHISELSWS
                     HVNHPSEILSIGDTVPVKVLDIDRDRQRISLGLKQTQEDPWQRVVDTYNIGDELEGKV
                     TKVVTFGAFVEIMDGVEGLVHISELAHHHVENPREVVEPGSEVKVKVLEIDSERRRLS
                     LSIKRVDGNEPPPDAGGSTGGSTLGRVPDLGLSDEMFAGPSVSGAEDLRAVVVATSEA
                     TPDEGGAEAPAAEPEASAADATPTTDAADAEAPADDSSSDDADATPEDAGAEAPAEAT
                     ADAGDEPDAAAETDES"
     gene            1064..1669
                     /gene="coaE"
                     /locus_tag="VHF88_00055"
     CDS             1064..1669
                     /gene="coaE"
                     /locus_tag="VHF88_00055"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX2160198.1"
                     /translation="MPGAGQVPFVGLTGGIGSGKSTALALLEELGATTLSADAVVHEL
                     LSSDDVRDALVSRLGGDVLDADGMVDRRAVAATVFERSDDRAWLEGMLWPRVGARIVE
                     WRAEADRLEPPPTAAVVEVPLLLEAGMEAVFDTTIAVGADEPLRVRRAEERGHAAVAE
                     RSARQLPQQEKAARAGHVVRNDGTREELKAELSRVLATIKE"
     gene            1772..2332
                     /locus_tag="VHF88_00060"
     CDS             1772..2332
                     /locus_tag="VHF88_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX2160199.1"
                     /translation="MVLAAVIGAAAAAAVGGLGPIGDAVREITLPLRHEDIIRQQSAD
                     KGLDPALVAAVIYEESRFRDQTSHAGARGLMQITPDTAKFIAQDSGGTAFTQADLATP
                     QVNISYGAYYLRYLLRKYDGNETLAVAAYNAGETNVNRWIEEAGGVDGFDASEDIPFP
                     ETQHYVENVRERKGEYREHYGDELGL"
     gene            2359..3723
                     /locus_tag="VHF88_00065"
     CDS             2359..3723
                     /locus_tag="VHF88_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2160200.1"
                     /translation="MTALVDDLGRLIGREYVREGAGAYGHDATEMRGVSGSADAVVLP
                     GSAEEVAALVAWCYERDLPIVPRGGGTGFAGGAVPLGDGVVLALDRLRAVRALEPELW
                     RMHVEAGLTTRHVQRVAREGGVWFPPDPGAAEQSQIGGNIATNAGGPHAFKYGVTGSW
                     VTGLEAVIAPGELIAVGGPLRKDVAGYDLRGLLVGSEGTLGIVTAAWLRLIPAPEAQL
                     PIAACYAGVAEGCRAVETVLASGVVPAVLEFLDGPALAAAGRSFPATLPGSAAFMVIA
                     EADGSRQQASADAAELMRALEPGAVALHAPTQPADTAALWRWREGVSLAVAAVRGGKL
                     SEDIVVPVDRLAEAIEGVAAIAAAHGLDSCSWGHAGDGNLHATFMVARDRDAFERAEA
                     ACQELFALALRLGGSISGEHGVGLVKSGQLRHQWPPAAVEAHRRLKLALDPKGLFNPG
                     KKQA"
     gene            3832..4149
                     /locus_tag="VHF88_00070"
     CDS             3832..4149
                     /locus_tag="VHF88_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160201.1"
                     /translation="MSAARSVAFDGAPLEREAPGIASRSIEVDGVRWAVVEFEPGISR
                     EEWCEEGHCGLVLEGEVVYELTAGDALRVVAGEAFALPEGDGHRARAGAAGARLFVID
                     RAP"
     gene            4213..6219
                     /gene="uvrB"
                     /locus_tag="VHF88_00075"
     CDS             4213..6219
                     /gene="uvrB"
                     /locus_tag="VHF88_00075"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX2160202.1"
                     /translation="MPRFELNSIYTPIADQPAARDALAAGVEAGDQFQTLLGVTGSGK
                     TATMAFTIEKLQRPALVIAHNKTLAAQLCNEFRELFPNNSVEYFVSYYDYYQPEAYVP
                     SSDLYIEKDSSINQEIERLRHAATASLLGRRDTIVVASVSCIFGLGSPEKYDQMMLLL
                     KRGGTVDRDEALRKLVDIRYTRNDSALSRGTFRVRGDTLEVFPAYAETAYRVDLFGDE
                     IEALKEFDPLTGEVISELEHAAVWPATHYATDQSTIERAVGEIRDELEAQCKLLEEQG
                     KPLESHRLRQRTQFDMEMLRELGFCNGIENYSRILDGRRPGERPYCLIDFFPDDFVCF
                     IDESHQTVPQIGGMYEGDRSRKQTLVDYGFRLPSALDNRPQKFDEFLSITPQIVFVSA
                     TPGDFERTRSARLVEQIVRPTGIVDPEVEVRETKNQIDDLMNEIRARSEAGERTLVTT
                     LTKKMSEDLTDYLMEYGVRVRYLHSEVDTLDRIQIIRELRLGEFDVLVGVNLLREGLD
                     LPEVSLVAILDADKEGFLRGETSLIQTIGRAARNVRGRVIMYADRESAAMRTAIDETN
                     RRRAIQVAYNEQHGITPETIRKGISDIGEFLTGAESKVPQGRRRKRKPDEEGMSAEQI
                     ARTLIELEEEMLAAAEELRFEYAAKLRDEIKILRRELDVLEAGT"
     gene            6248..6538
                     /locus_tag="VHF88_00080"
     CDS             6248..6538
                     /locus_tag="VHF88_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160203.1"
                     /translation="MDEQLKRLAERAASLEDQARELREALDELVASEADAELAVAAAE
                     SEAPDDSEARIVAYSMVLDGRPREEVARHLAQELGLTDSGALLDDLYARAGE"
     gene            6629..>6982
                     /locus_tag="VHF88_00085"
     CDS             6629..>6982
                     /locus_tag="VHF88_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160204.1"
                     /translation="MTPPKTGGAADEIVVAGAREHNLKDIDIRLPRNALVVITGLSGS
                     GKSSLAFDTIYAEGQRRYVESLSAYARQFLGQMDKPDVDSIEGLSPAISIDQKTTSRN
                     PRSTVGTVTEIYDYLR"
BASE COUNT         1061 a         2397 c         2554 g          970 t
ORIGIN      
        1 ctcggcgtgc gcggcttcct gccggcgtcg ctcgtcgaca tccggcgcgt ccaggacctg
       61 gacgagttca tgggccagaa gatcgagtgc aaggtcatcg agctcaaccg gtcgcgcaac
      121 aacgtcgtgc tgtcgcgccg cgcggtgctc gaggaggagc gcaaggaggt ccgccagcag
      181 atcctggacc gcctgcagcc gggccaggtg gttgagggct cgatctcgaa catcgtcgac
      241 tgcggcgcgt tcgtcgacct cgacggcatc gacggcttga tccacatctc cgagctctcg
      301 tggagccacg tcaaccatcc gtcggagatc ctctcgatcg gagacaccgt gccggtcaag
      361 gtgctcgaca tcgaccgcga tcgtcagcga atctcactgg gtctcaagca gacccaagag
      421 gacccgtggc agcgcgtggt cgacacctac aacatcggcg acgagctcga gggcaaggtc
      481 accaaggtcg tcacgttcgg cgcgttcgtc gagatcatgg acggcgtcga gggcctcgtc
      541 cacatctccg agctcgccca ccatcacgtc gagaacccgc gcgaggtcgt cgagccgggc
      601 tccgaggtca aggtcaaggt gctcgagatc gactccgagc gccgccgcct gtcgctgagc
      661 atcaagcgcg tcgacggcaa cgagccgccg ccggatgccg gcggaagcac gggcggcagc
      721 acgcttggcc gcgttccgga ccttggcctt tcggacgaga tgttcgccgg accgtcggtg
      781 agcggcgccg aggacctgcg cgcggtcgtc gtcgcgacgt cggaggcgac tcccgacgag
      841 ggcggcgccg aggctccggc cgccgagccg gaggcgagcg ccgccgatgc gacgccgacc
      901 accgatgctg ccgacgccga ggcgcctgcc gacgactcga gctccgatga cgcggacgcg
      961 acgccggagg atgccggcgc ggaggcgcct gcagaggcca cggccgacgc cggcgacgag
     1021 ccggacgccg ccgccgagac agacgagagc tagggagccc gacatgccgg gcgccggcca
     1081 ggtcccgttc gtcgggctga ccgggggcat cggctcgggc aagtcgaccg cactggccct
     1141 gctcgaggag ctcggcgcga ccacgttgtc ggccgacgcc gtcgtgcatg agctcctgag
     1201 cagcgacgac gtgcgcgacg cgctggtctc gcggctcggc ggcgacgtgc tcgacgccga
     1261 cgggatggtc gaccgtcgcg cggtcgcagc gacggtcttc gagcgctccg acgatcgtgc
     1321 ttggctggag gggatgctgt ggccgcgggt cggcgcccgc atcgttgaat ggcgcgctga
     1381 ggcagaccgg ctcgagcccc ctccgacggc cgcggtcgtc gaggtgccgt tgctcttaga
     1441 ggccgggatg gaggccgtgt tcgacacgac gatcgcggtg ggcgccgacg agccgctgcg
     1501 cgtccggcgt gcggaggagc gaggccacgc cgccgtggcg gagcgctcgg cgcgccagct
     1561 cccgcagcag gagaaggcgg ctcgagcggg gcatgtggta cgcaacgacg ggacccgaga
     1621 ggagttgaag gcggagctgt cccgcgttct tgccacgatc aaggagtaga ccttggcctc
     1681 tggctctgcc acccgctcgc gatcgcgccg gcaagcgtcc gctcggcggt ctgcgcgtgc
     1741 gcggacgaca cggcggcgca ggatcgccgc cgtggtgctg gccgcggtga tcggcgccgc
     1801 agccgccgca gcggtcggag ggctcgggcc gattggcgac gccgtgcggg agatcacgct
     1861 gccgttgcgg cacgaggaca tcattcgcca gcagtcggcc gacaagggcc tcgacccggc
     1921 gctggtcgcg gcggtcatct acgaggagtc gcgcttccgc gaccagacct cgcacgctgg
     1981 cgcccgcggc ctgatgcaga tcacgcccga cacggcgaag ttcatcgccc aggactcagg
     2041 gggcaccgcc ttcacccagg ccgacctggc cactccgcag gtgaacatct cctacggcgc
     2101 ctactacctg cgctacctgc tgcgcaagta cgacggcaac gagacgttgg cggtcgccgc
     2161 ctacaacgcc ggcgagacga acgtcaaccg gtggatcgag gaggcgggtg gggtagacgg
     2221 cttcgacgcc tccgaggaca tcccgttccc cgagacccag cattacgtgg agaacgtgcg
     2281 cgagcgcaag ggcgagtacc gcgagcacta cggcgacgag ctcggcctct aggcgagggg
     2341 ccggcggatc gctcgcgcat gaccgccctc gtcgacgacc tcggccggct gatcggtcgc
     2401 gagtatgtgc gcgagggcgc gggtgcgtac gggcacgacg cgacggagat gcgcggcgtc
     2461 tcggggtcgg ccgatgctgt tgtcctgccg ggcagcgccg aggaggtggc tgcgctcgtc
     2521 gcgtggtgct acgagcgcga cctgccgatc gtcccgcgcg gcggcggcac cggattcgcg
     2581 ggcggcgcgg tcccgctcgg cgacggagtg gtgctcgcgc tcgaccggct gcgcgccgtg
     2641 cgcgcgctcg agcccgagct gtggcggatg catgtcgagg ccggcctgac gacgcgtcac
     2701 gtccagcgag tggcgcgcga gggcggcgtc tggtttccgc ccgacccggg tgccgcggag
     2761 cagtctcaga tcggcggcaa catcgcgacc aacgcgggcg ggccgcacgc cttcaagtac
     2821 ggcgtcaccg gttcgtgggt cacggggctc gaggcggtga tcgctccggg tgagctgatc
     2881 gcggtcggag gtccgctgcg caaggacgtc gccggctacg acctgcgcgg cctgctggtc
     2941 ggatccgagg gcacgctcgg catcgtgacc gcggcgtggc tgcgactgat cccggccccc
     3001 gaggcgcagc tgccgatcgc ggcctgctat gcgggcgtcg cagaaggctg ccgggcggtc
     3061 gaaacggtgc tcgccagtgg cgtcgtcccg gccgtgctcg agttcctcga cggcccagcg
     3121 ctcgcagcgg cggggcgatc gtttcccgcc acgctgcccg gctcggcggc cttcatggtg
     3181 atcgccgagg ccgacggctc gcgacaacag gcgagcgccg acgcggccga gctgatgcgg
     3241 gcgctcgagc cgggcgcggt cgcgctgcac gcgccgacgc agcccgccga cacggcggcg
     3301 ctctggcgct ggcgcgaggg ggtctcgctg gccgtcgcgg ccgtccgcgg cggcaagctg
     3361 tccgaggaca tcgtcgtgcc ggtcgaccgc ctcgcggagg cgatcgaggg cgtcgcggcg
     3421 atcgccgcgg cgcacggcct cgactcgtgc agctggggcc acgccggcga cgggaacctt
     3481 cacgcgacgt tcatggtggc gcgcgatcgc gacgcgttcg agcgcgccga ggccgcctgc
     3541 caggagctgt tcgcgctcgc gctgcgactc ggcggctcga tctcgggcga gcatggcgtc
     3601 gggctggtga agagcggtca gctgcgccac cagtggccgc ccgcggccgt cgaggcccac
     3661 cggcggctga agctggcgct cgacccgaag ggtctcttca acccgggcaa gaagcaggcc
     3721 tgacgctcgt ccaagcgacg aattggttgc gcccggtttg gagcgtctcc aatggctcgg
     3781 cgcgctgggg ccagccacga tcggcgacat gggcagcctg gtcgggaagc cgtgagcgcc
     3841 gcgcgcagcg tcgctttcga cggcgcaccg ctcgagcgcg aggcgcccgg gatcgcttcg
     3901 cgctcgatcg aggtcgacgg cgtccgctgg gcggtcgtcg agttcgagcc cggcatctcg
     3961 cgcgaggagt ggtgcgagga ggggcactgc ggcctggtgc tcgagggcga ggtggtctac
     4021 gagctgaccg ctggtgatgc gctgcgcgtg gtcgcgggcg aggccttcgc gctgcccgag
     4081 ggtgacgggc accgggcgcg cgccggggcc gctggggcga ggctcttcgt gatcgatcgc
     4141 gcgccctgag acggcgaacg cgccggccga ccggaccggc ggcgcctggc gctgctgcta
     4201 gcttcggtcg ccgtgccgag attcgagctc aactcgatct acacgccgat cgcggaccag
     4261 cctgcggcgc gcgacgccct ggccgcgggc gtcgaggccg gcgaccagtt ccagacgctg
     4321 ctcggcgtca ccgggtccgg caagacggcg acgatggcgt tcacgatcga gaagctgcag
     4381 cgaccggcgc tggtgatcgc gcacaacaag acgctcgccg cgcagctctg caacgagttc
     4441 cgcgagcttt tcccgaacaa ctcggtcgag tacttcgtct cctactacga ctactaccag
     4501 cccgaggcct acgtcccgtc gtcggacctc tacatcgaga aggactcctc gatcaaccag
     4561 gagatcgagc ggctgcgcca tgccgccacc gcgtcgctgc tcggacggcg cgacacgatc
     4621 gtcgtcgcga gcgtgtcgtg catcttcggc ctcggctctc ccgagaagta cgaccagatg
     4681 atgctgctgc tcaagcgcgg cggcacggtc gaccgcgacg aggcgctgcg caagctcgtc
     4741 gacatccgct acacgcgcaa cgacagcgcg ctgtcgcgcg gcaccttccg cgtccgcggc
     4801 gacacgctcg aggtcttccc ggcctacgcc gagaccgcct accgcgtcga cctcttcggc
     4861 gacgagatcg aggcgctgaa ggagttcgac ccgctgaccg gcgaggtgat ctccgagctc
     4921 gagcacgccg ccgtctggcc ggcgacccac tacgcgaccg atcagagcac gatcgagcgc
     4981 gcggtcggcg agatccgcga cgagctcgag gcgcagtgca agctgcttga ggagcagggc
     5041 aagccgctgg agtcgcaccg gttgcgccag cggacgcagt tcgacatgga gatgctgcgc
     5101 gagctcggct tctgcaacgg gatcgagaac tactcgcgga tcctcgacgg ccgccgtccg
     5161 ggcgagcggc cgtactgcct gatcgacttc ttcccagacg acttcgtgtg cttcatcgac
     5221 gagtcgcatc agaccgtccc gcagatcggt gggatgtacg agggtgaccg ctcgcgcaag
     5281 cagacgctcg tcgactacgg cttccggctg ccgagcgcgc tcgacaaccg gccgcagaag
     5341 ttcgacgagt tcctctcgat cacgccgcag atcgtcttcg tctcggcgac gccgggcgac
     5401 ttcgagcgga cccgctcggc tcgcctggtc gagcagatcg tgcgcccgac cgggatcgtc
     5461 gaccccgagg tggaggtgcg cgagaccaag aaccagatcg acgacctgat gaacgagatc
     5521 cgcgcgcgca gcgaggccgg cgagcggacc ctggtcacga cgctgaccaa gaagatgtct
     5581 gaggatctga ccgactacct gatggagtac ggcgtccggg tccgctacct gcactccgag
     5641 gtcgacacgc tcgaccggat tcagatcatc cgcgagctgc ggctcggcga gttcgacgtg
     5701 ctggtcggcg tcaacctgct gcgcgagggg ctcgacctgc cggaggtctc gctcgtcgcg
     5761 atcctcgacg cggacaagga gggcttcctg cgcggcgaga cctcgctgat ccagacgatc
     5821 ggccgcgccg cccgcaacgt ccgcggccgg gtgatcatgt atgccgatcg tgagtcggcc
     5881 gccatgcgca ccgcgatcga cgagaccaac cgccgccgcg cgatccaggt cgcctacaac
     5941 gagcagcacg ggatcacgcc ggagacgatc cgcaagggga tctccgacat cggcgagttc
     6001 ctcaccgggg cggagtcgaa ggtcccgcag ggacgccggc gcaagcgcaa gcccgacgag
     6061 gagggcatgt cggccgagca gatcgcccgc acgctgatcg agctcgagga ggagatgctg
     6121 gccgcggcgg aggagctgcg cttcgagtac gccgccaagc tgcgcgacga gataaagatc
     6181 ctgcggcgcg agctggacgt gctggaggcc ggcacctgag gcgggaccgg tggctacgat
     6241 ccggccggtg gacgagcagc tgaagcggct ggcggagcgc gcggcgagcc tggaggacca
     6301 ggcgcgcgag ctgcgcgagg cactcgacga gctggtggcc tcggaggcgg acgccgagct
     6361 cgccgtcgcg gcggccgagt cggaggcccc ggacgactcc gaggcgcgga tcgtcgccta
     6421 cagcatggtg ctcgacggca ggccgcgcga ggaggtcgcg cggcacctcg cgcaggagct
     6481 cggactcacc gacagcgggg cgctgctcga cgacctctac gcccgtgccg gggagtagcg
     6541 gccggccagg cgcctccggg gtcagggggc gtgagccctt gggctctatc cttaccgaac
     6601 gtctgttcgc acggctagac tgcttcgcgt gacgcctccg aagacgggcg gggcggctga
     6661 cgagatcgtc gtcgccggcg cgcgcgagca caacctcaag gacatcgaca tccggctgcc
     6721 ccggaacgcg ctcgtggtga tcacgggcct ctccggctcc ggcaagtcga gccttgcctt
     6781 cgacacgatc tacgcggagg ggcagcgccg ctacgtcgag tcgctctccg cctacgcgcg
     6841 ccagttcctc ggccagatgg acaagcccga cgtcgactcg atcgagggcc tctcgccggc
     6901 gatctcgatc gaccagaaga cgacctcgcg caacccgcgc tcgacggtcg gcacggtcac
     6961 cgagatctac gactacctgc gc
//
LOCUS       DATABV010000005         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2572441, whole genome shotgun sequence.
ACCESSION   DATABV010000005 DATABV010000000
VERSION     DATABV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572441"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VHF88_00090"
     CDS             <1..1273
                     /locus_tag="VHF88_00090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2160205.1"
                     /translation="NLLDDQRPEEIAFPTDEREVAVAIADARERGLRVAAQATGHNAG
                     PLGSLEGTLILNTSALTGVSIDADARRVRVGAATKWEKVTPQLSELGLAGLHGSSPDV
                     GIVGYSLGGGIGWLARKHGMQTNAVTAIELVTAEGHLVRTDAVHEPDLFWALRGGGGN
                     FGVVTAIEFAVQPVRELYAGALFFEVEQTSDVLHTWTKLLPSLPEEITSWASILHFPP
                     LPEFPAIVRGRSFVVVMAAFLGSEADGRELLRPLRRLGPEMDTFAIQPPIALAELAMD
                     PPDPLPVRSAHALIDGLPSEAIESVTRTAGPGSALGMVQFRHMGGALARREPGAGARA
                     TLPGQIAMFSLGVVPEPAADPVVLDELEAVSAAVAPHAVGEYPNFVEEPTDASSFFDA
                     ETWERLRHVKALYDPQDVFKGNHHVPPAERS"
     gene            1309..1782
                     /locus_tag="VHF88_00095"
     CDS             1309..1782
                     /locus_tag="VHF88_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160206.1"
                     /translation="MSKATSSIVVAVTLGLLAAAPAADAAKGVDYKGRTAGGHPISFR
                     LHEGVARNFATGVPMNCIAIQGGGSPLTGVEAVSFSFVEVGIRDYKYSEQVKPSFHYN
                     EVTRNHTMTLLRAGKRVIKGALRTQYSFLIPKYPIGTFSVYSCLGNTKFRARPTR"
     gene            1808..2932
                     /locus_tag="VHF88_00100"
     CDS             1808..2932
                     /locus_tag="VHF88_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160207.1"
                     /translation="MTTATRRLGATLGLALAILAAGPAAAQALTVSEYEVTIEGEATY
                     ARSDAFPGPFGPWEQREQAEFAWRTRFPSVRFLDKQVGIASQSETTVSNVNAELYTSV
                     PTPEGPITGACTGTTLADPPGSGWLGASVIPTPDPNVEALDVRVLGGVSFHLPNCSGS
                     FGGGPAQSLRIGSEAEIPWGPFDQAFDLPHEAVGMGKIIQLLNATVTGERCPGHGFET
                     ASCALAWKATVTFVRTAHWRTDLDESDVPLPPKPQPGPAPGGSQILPPFEIPLPKRAR
                     LSAGGERARLTLTCPVACAGTAAAYPARRGRRARGAAVRPLARARFRAAARRPTTVVL
                     RFGPRARREVRRAGGVRVDLRVAATAGGPAARRSVVMRRP"
     gene            2978..3346
                     /locus_tag="VHF88_00105"
     CDS             2978..3346
                     /locus_tag="VHF88_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2160208.1"
                     /translation="MHIDKDLVAASATPLVLAILAEGESYGYAILKRVRELSEGEMEW
                     TDGMLYPLLHRLRRLGYVTTEWRTPPEGRRRRYYAITDEGRATLAEQQRQWLTVTRAL
                     GDVWRGSAALPRALGEGVTS"
     gene            3343..>4426
                     /locus_tag="VHF88_00110"
     CDS             3343..>4426
                     /locus_tag="VHF88_00110"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX2160209.1"
                     /translation="MSGVESQIAEWRAYVVEAPAVNGRDVDELEAHLRDEIAELDAAG
                     LTADEAFLVAVKRMGDLDTLSREFAREHSGRLWKQLVLSSEDEPARPSGEWAEALAFA
                     LAAAVAIQIARLAAGFPGEEPRWFAMNLSLIVLPFLAGYFARRRRLDVRGWALTAAPF
                     VLGALVVNLYPYSPESATEPLVALHLPVALWFAVAYPYMGGTIRSHERRMDFVRFTGE
                     WIIYYVLIALGGGVLVGLTALILEPIGPGLAETVVEWVLPSGAAGAVIVAAWLVEAKQ
                     RVVENMAPVLTMVFTPLFAVMLAIAAATYAVSGFAGAFDRELLGVFDALLLVVLGLVL
                     YAMTAREPARRPGLMDRVQLLAVASAL"
BASE COUNT          604 a         1611 c         1573 g          638 t
ORIGIN      
        1 caacctgctg gacgaccagc gccccgagga gatcgccttc cccaccgacg agcgcgaggt
       61 cgcggttgcg atcgccgacg cccgcgagcg cggcctgcgc gtcgccgcgc aggcgacggg
      121 gcacaacgcc ggtccgctcg gatcgctcga gggcacgctg atcctgaaca cgtccgcgct
      181 gaccggcgtg tcaatcgacg cggacgcgcg ccgcgtccgc gtcggcgcgg cgacgaagtg
      241 ggagaaggtg actccccagc tctccgagct tggcctcgcg ggcctgcacg gctcctcgcc
      301 ggacgtcggg atcgtcggct actcgctcgg cggcggcatc ggctggctcg cgcgcaagca
      361 cggaatgcag accaacgcgg tgaccgcgat cgagctcgtc accgcggagg gccatctcgt
      421 gcgcaccgac gcggtccacg agcccgacct gttctgggcg ctgcgcggcg gcggcggcaa
      481 cttcggcgtc gtgaccgcga tcgagttcgc ggtccagcca gtgcgcgagc tgtacgccgg
      541 cgcgctgttc ttcgaggtcg agcagacctc cgacgtgctg cacacgtgga cgaagctgct
      601 gccgtcgctg ccggaggaga tcacctcgtg ggcgagcatc ctccacttcc cgccgctgcc
      661 ggagttcccg gcgatagtcc gcgggcgctc gttcgtcgtg gtcatggcgg cgttcctcgg
      721 ctcggaggca gacgggcgcg agctgctgcg gccgctgcgg cggctcggac ccgagatgga
      781 cacgttcgcg atccagccgc cgatcgcgct cgccgagctg gcgatggacc caccggatcc
      841 gctgccggtc cggagcgcgc atgcgctgat cgacgggctg ccctccgagg cgatcgagtc
      901 cgtcacgcgg accgccggtc ccgggtcggc tcttgggatg gtccagttcc ggcacatggg
      961 cggcgcgctg gcgcggcgtg agccgggcgc cggagcgcgc gccacgctgc ccggccagat
     1021 cgcgatgttc agcctcggag tcgtgccgga gcccgcggcc gacccggtgg tcctcgacga
     1081 gctcgaggcg gtgtcggccg ccgtcgcgcc gcatgcggtc ggcgagtacc ccaacttcgt
     1141 cgaggagccg acagatgcga gctccttctt cgacgccgag acctgggagc gcctccggca
     1201 cgtcaaggcg ctctacgacc cgcaggacgt cttcaagggc aaccaccacg tcccgccggc
     1261 ggagcggtcc tgagaccggc cccacaccag cgagcaaagg agagcaccat gagcaaggcc
     1321 acttcatcca tcgtcgtcgc cgtcaccctc gggctgctag ccgccgcccc ggcggcggac
     1381 gccgccaagg gcgtcgacta caagggcagg acggccggcg gccacccgat cagcttccgc
     1441 ctgcacgagg gggtcgcgcg caacttcgcc acgggcgtgc cgatgaactg catcgcgatc
     1501 cagggcggtg gctcgccgtt gacgggcgtc gaggccgtca gcttcagctt cgtggaggtg
     1561 gggatcaggg actacaagta ctcggagcag gtcaagccga gctttcacta caacgaggtc
     1621 acacgcaatc acacgatgac cctcctgcgg gccggcaagc gggtgatcaa gggcgcgctg
     1681 cggacccagt actcgttcct gatcccgaag tacccgatcg gcacgttcag cgtctacagc
     1741 tgcctcggca acaccaagtt ccgcgcccgg ccgacacgct gagcgcccga tcgagaggag
     1801 agtcgcgatg acgaccgcaa ccaggcgcct cggcgccacc cttggcctcg cattggcgat
     1861 cctcgccgcc gggccggcag ccgcgcaggc gctgaccgtg tccgagtacg aggtgacgat
     1921 cgagggcgag gcgacctacg cgcgcagtga cgccttcccc gggcccttcg gcccgtggga
     1981 gcagcgggag caggcggagt tcgcttggcg gacccgcttt ccgtcggtga ggttcctcga
     2041 caagcaggtc gggatcgcct cccagtccga gacgacggtg tcgaatgtca acgccgagct
     2101 gtacacgagc gtcccgacgc cggagggccc gatcaccggg gcctgcaccg gcacgacgct
     2161 ggcggacccg cccggctccg ggtggctggg agcctcagtc ataccgactc cggacccgaa
     2221 cgtcgaggcg ctcgacgtgc gcgtgctcgg cggcgtgagc ttccacctgc cgaactgctc
     2281 gggctcgttc ggcggcggtc ctgcgcagtc gctccggatc ggcagcgagg ccgagatccc
     2341 ctggggcccg ttcgaccagg cattcgacct gccccacgag gcggtcggaa tggggaagat
     2401 cattcagctg ctgaacgcca cggtcaccgg cgagcgctgc ccggggcatg gcttcgagac
     2461 cgcctcgtgc gcgctcgcct ggaaggccac cgtgacgttc gtgcgcaccg cgcactggcg
     2521 gaccgacctc gacgagagcg acgtccctct gccgccgaag ccgcagcccg ggcccgcgcc
     2581 gggcggctcg cagatcctgc ccccgttcga gatcccgctg cccaagcgcg ccaggctgag
     2641 cgccggtggt gagcgggcgc gattgacgct cacctgcccg gtggcttgcg ccggcacggc
     2701 ggccgcctac ccggcacggc gaggacgtcg cgcccgcggc gcggcggtcc gcccactcgc
     2761 gcgcgcccgc ttcagggccg ctgcgcgccg tccgaccacc gtcgtgctgc gcttcggccc
     2821 gcgggcgcga cgcgaggtcc gtcgagcagg cggcgtgcgg gtcgatctgc gcgtcgcagc
     2881 gaccgccgga ggaccggccg ctcgaaggag cgtcgtgatg cgccgaccct gataccttgc
     2941 agctctatgc ctaacgcctc gacccttccg ggcgcgcttg cacatcgata aggatctcgt
     3001 cgcggcctcc gcgacgcccc tggtccttgc catcctggcc gagggcgaga gctatggcta
     3061 cgcgatcctc aagcgggtgc gcgagctgtc ggaaggcgag atggagtgga ccgacgggat
     3121 gctctacccg ctgctccacc gcctccgccg gctcggctac gtgacgacgg aatggcggac
     3181 tccgcccgag gggcggcggc gcaggtacta cgcgatcacc gacgaggggc gggcgacact
     3241 cgccgagcag caacggcagt ggctgaccgt gacgcgcgcg ctgggcgacg tctggcgcgg
     3301 ctcggcggcg ttgccgaggg cactcgggga gggcgtgacc tcatgagcgg cgtggagtca
     3361 cagatcgcgg agtggcgtgc ctatgtcgtc gaggcgcccg ccgtcaacgg ccgcgacgtc
     3421 gacgagctcg aggcgcacct gcgcgacgag atagccgagc tcgacgcggc cggcctgacg
     3481 gccgacgagg cgttcctcgt cgccgtgaag cggatgggcg acctcgacac cctctcgcgc
     3541 gagttcgcac gcgagcacag cggccggctc tggaagcagc tcgtcctgag cagcgaggac
     3601 gagccggcgc ggccgtccgg cgagtgggcc gaggcgctcg ccttcgcgct ggccgcggcc
     3661 gtcgcgatcc agatcgcgcg cctcgccgcc gggttccccg gcgaggagcc gcgatggttc
     3721 gcgatgaacc tcagcctgat cgtgctgccg ttcctggcgg gctacttcgc ccgccgccgg
     3781 cggctcgacg tccggggatg ggcgctcacc gccgcgccgt tcgtgctcgg cgccctcgtc
     3841 gtcaacctct acccgtacag ccccgagtcg gccaccgagc cgctcgtcgc cctccacctg
     3901 cccgtcgccc tctggttcgc cgtcgcctat ccgtacatgg gcggcacgat ccgctcccac
     3961 gagcgacgga tggacttcgt ccgcttcacc ggcgagtgga tcatctacta cgtcctgatc
     4021 gcgctcggcg gcggcgtcct ggtcgggctg accgcgctga tcctcgagcc gatcgggccc
     4081 ggcctcgccg agacggtcgt cgagtgggtg ctgccgtctg gcgctgccgg cgccgtgatc
     4141 gtggcggcgt ggctcgtcga ggcgaagcag cgcgtggtcg agaacatggc gccggtcctc
     4201 acgatggtct tcaccccgct gttcgccgtg atgctggcga tcgccgccgc cacctatgcc
     4261 gtgtccgggt tcgccggcgc gttcgaccgc gagctgctcg gcgtcttcga cgcgctccta
     4321 ctggtcgtgc tgggactcgt cctctacgcg atgaccgcgc gcgagccggc ccgacggccc
     4381 gggctgatgg accgcgtcca gctgctcgcc gtcgcgagcg cgctcg
//
LOCUS       DATABV010000006         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2251367, whole genome shotgun sequence.
ACCESSION   DATABV010000006 DATABV010000000
VERSION     DATABV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251367"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VHF88_00115"
     CDS             complement(<1..558)
                     /locus_tag="VHF88_00115"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX2160210.1"
                     /translation="MPTDEQIKEALSRVIDPELRKNIVELGMVRSVNQHDDGHVEVVV
                     SLTTAGCPIRSHFEDAVAEHVGALDGVTRVSVGFDVLTPDEKQSLQQRLGRGALPQGA
                     LAQVKNVICVGSGKGGVGKSTLTANLAAALQQEGMQAAALDADVWGYSIPRMLGVHGR
                     PDIDGQRKILPLEAAGGVKVISIEFF"
     gene            complement(705..1226)
                     /locus_tag="VHF88_00120"
     CDS             complement(705..1226)
                     /locus_tag="VHF88_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160211.1"
                     /translation="MAEQTPTQRKATAKKAAATRKRNAATRSAKTAKSSAKATQTSAR
                     TAASNATSAGNLTLDTVALQAERAVLIPVGAALAARDAVVEATKPFTSRSSAEKELRK
                     FERRGNTARNNVRREVKRTRTRVERELRQRRNDVRTRVVDVRGDLQKQVSEVQSNATS
                     VARKVQEQVAALA"
BASE COUNT          227 a          600 c          648 g          304 t
ORIGIN      
        1 gaagaactcg atcgagatca ccttgacgcc accggccgcc tcgagcggca ggatcttgcg
       61 ctggccgtcg atgtcggggc ggccgtggac gccgagcatg cgcgggatcg agtagcccca
      121 gacgtcggcg tcgagcgccg ccgcctgcat gccctcctgc tggagcgcgg cggcgaggtt
      181 cgcggtcagc gtcgacttgc cgacgccgcc cttgccggag ccgacgcaga tcacgttctt
      241 gacctgggcg agcgcgccct gcggcagcgc gccgcggccg agccgctgct ggagcgactg
      301 cttctcgtcg ggcgtgagga cgtcgaagcc gacgctgacg cgcgtcacgc catcgagcgc
      361 gccgacgtgc tcggcgacgg cgtcctcgaa gtgcgagcgg atcgggcagc cggccgtcgt
      421 gagcgagacg acgacctcga cgtggccgtc gtcgtgctgg ttcaccgagc ggaccatccc
      481 cagctcgacg atgttcttgc gcagctccgg gtcgatgact cggctcagcg cctccttgat
      541 ctgctcgtcg gtaggcatct ctttcggatt gtcgcagagt cgtttccaag gcccgggccc
      601 cagaacgacg aagggccccc gatccgggag cccttcgctg gggaggagga aacggacttc
      661 gtccgtgcgc acccggcaga gccgagcgct tcggcagggc gccgttacgc cagcgcggcg
      721 acctgctcct ggaccttgcg ggcgacgctg gtcgcgttcg actggacctc ggagacctgc
      781 ttctggaggt cgccgcggac gtcgaccacg cgcgtgcgga cgtcgttgcg acgctgacgc
      841 agctcgcgct cgacgcgcgt gcgggtgcgc ttgacctcgc ggcggacgtt gttgcgggcg
      901 gtgttgccgc gccgctcgaa cttgcgcagc tccttctcgg ccgagctgcg gctggtgaac
      961 ggcttggtcg cctcgacgac ggcgtcgcgg gcggccagcg cggcaccgac cgggatcagc
     1021 acggcacgct cggcctgcag cgcgacggtg tcgagcgtca ggttgccggc gctcgtcgcg
     1081 ttggacgcgg cggtccgggc ggacgtctgg gtcgccttcg ccgagctctt ggcggtcttg
     1141 gcgctgcgcg tggcggcgtt gcgcttgcgg gtcgcggcgg ccttcttggc ggtcgccttg
     1201 cgctgcgtgg gggtctgctc tgccatctag gtgtggctcc ttccggggtg ctcgagtacg
     1261 agtcgaagct tgttagatct aagttcggta gaactgtacc accgaacaca cgtttgtaaa
     1321 ccgaatggcc ggcggggtta cgaccagccc gccgggggac cacttcgcgg ccgttcttcc
     1381 gctatcgggt ccgatgtctt catagatccg tccaaggcgc cgaaacggcc cttgacttct
     1441 cccgaacgcg tggataagtt cgtgtctcga tccagcgacg ggcgaggaaa tcacctcttc
     1501 tggctccctg atggagggca gggaaggtgc cgaatcggcc ccaatccggc caccgcccgc
     1561 ccggccggac ggcatcgcga atggagggcg cgagccaccg gggcgcctgg tcgcacgggc
     1621 cggcggttgg agcttcggct ccgccgccgg cctccagaag gcctgcgcgc cgtcagcgct
     1681 tcgcgcgctt gcgcgtctgg gcctcggcgg ccgcggcgat ctcggacacg cgcaggtcga
     1741 gcgcgtcggc gagggcggtg atcttctccc aggtcgggc
//
LOCUS       DATABV010000007         3531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1368342, whole genome shotgun sequence.
ACCESSION   DATABV010000007 DATABV010000000
VERSION     DATABV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368342"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VHF88_00125"
     CDS             <1..551
                     /locus_tag="VHF88_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160212.1"
                     /translation="DLVGEGVPADLAGACARLALGNASRARFLASEEGAALRADVDRL
                     VAAALAGGTRHANEPEPWRGLGGEPKGRDRSAIEKQFDDAAKRDGRRARTELLDLGLT
                     LAALRFRDLVCLAEGADDAVLDRAGAATLATAAPQVASSDLRAAAERCEDVRMSLELN
                     VTEDLALEALGFRLARLVGAPA"
     gene            complement(548..907)
                     /locus_tag="VHF88_00130"
     CDS             complement(548..907)
                     /locus_tag="VHF88_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX2160213.1"
                     /translation="MRSAKGGGRRAIGPYIGVVPARLSRLEQLSLPGCPRVFVARTAR
                     TRLLGLAWLDDMPRDCALLIPHCSSVHTFGMRFALDVCFLDGEGLVVRRERAVPPRRF
                     VTCSGAAAVLERRADPG"
     gene            1030..3051
                     /locus_tag="VHF88_00135"
     CDS             1030..3051
                     /locus_tag="VHF88_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX2160214.1"
                     /translation="MEPTQTEVPNAVEGWQPTLDPVTFEVLKNSFSTIVDQMAEQILR
                     TCYSFVIYAKDFSSALTDHEGNTVMQGSHDVAVHVGTLHFSAKAVLEIFGDEMAPGDV
                     YAMNDPYLGGTHFNDVRLIRPVFVDGELIAMVQCNGHWSDVGGSVPGSHDITAREHFQ
                     EGLRITPMKIWDKGVFRDDVVRMIVSNTRTPVDCEGDLHAQAEATRVAESEILRLVEK
                     YGKDTVKTAFADVQNYVETIMRGRLAGLPDGSWEAEDYIDFDPAAGEEGLIPVKVKMT
                     IDGNDLRYDLTGSHPVIGSALNCAFGMGFSGVVVGTKTFFPDIPLNSGFYRAMDVDLG
                     PVGSVVNAPHPVAVTGFNAGAYEKIVNAIFELWSGLMPERALACSYNLEYLLVGGRDG
                     RDPERNIFMWYDWMAGGWGGRNGKDGLNGTSPIFGAGLEVQPLEGQERLSPVLTTRHE
                     FIPDSGGPGRFRGGVGVEKGAILQQAESTVMSYSCDRARSITWGIGGGLPSNPHGVWL
                     DPDGPERHYLGAWFASMPAAKGTTFTRPSAGGGGYGDPLERDPNAVREDVADGYVTIE
                     RAAKDYGVVIRTVDEDLAAYEVDEAATLAERERIAANRLGWIEEDPESVAERYRAGEL
                     DVFDCVRQHGVVLDWGTGELLRNTTEQFRAMLARRTTAFWDAPAAAGAA"
     gene            3084..>3531
                     /locus_tag="VHF88_00140"
     CDS             3084..>3531
                     /locus_tag="VHF88_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2160215.1"
                     /translation="MPLPPERCDVAVVGAGIVGLSVARELTRRDPGASVAVIERERRL
                     AAHQTSHNSGVVHAGIYYRPGSLKAKLCVTGARELREYCERRAIPYREVGKVIVALDE
                     SELPALDGLQARAVANGVPRVERIGRERLRELEPAVRGIAALHSPAT"
BASE COUNT          522 a         1307 c         1261 g          441 t
ORIGIN      
        1 aggatttggt gggggagggc gtgccagccg acctggccgg cgcctgcgcg cgcctcgcgc
       61 tcggcaacgc gtcccgggcg cggttcctcg cgtcggagga gggcgccgcg ctgcgcgccg
      121 acgtcgaccg gctggtcgcc gccgcgctcg ccggcgggac ccgccatgcg aacgagccgg
      181 agccgtggcg cggcctcggc ggcgagccga agggccgtga ccgcagcgcg atcgagaagc
      241 agttcgacga cgccgccaag cgcgacggcc gccgcgcgcg cacggagctg ctcgaccttg
      301 gcctgacgct cgcggcgctg cgcttccgcg acctggtctg cctcgccgag ggcgccgacg
      361 acgcggtgct cgaccgcgcg ggcgcggcca cgctggccac cgccgcgccg caagtcgcct
      421 ccagcgacct ccgcgccgcc gccgagcgct gcgaggacgt gcgcatgtcg ctcgagctca
      481 acgtcaccga ggacctcgcg ctcgaggcgc tcggcttccg cctcgcccgc ctcgtcggcg
      541 ccccggccta gcccgggtcg gcgcggcgct cgaggaccgc ggcagcgccg ctgcatgtca
      601 cgaagcggcg cggagggacg gcgcgctcgc gccgcacgac cagcccttcg ccgtcgagga
      661 agcagacgtc aagcgcgaag cgcatgccga aggtgtggac cgagctgcag tgcgggatca
      721 gcagcgcgca gtcacggggc atgtcgtcca gccaagcgag cccgagcagc cgcgtccgtg
      781 ccgtgcgggc gacgaagacg cgcggacagc cgggcagcga cagctgctcg agccggctga
      841 ggcgggcggg caccacgccg atgtagggac cgatcgcgcg ccgcccgccc cctttggccg
      901 atcgcaccga agccccccgc tcctttcgtg cgaacgccaa ttgccgctgc cgccaccgcg
      961 gcccaacatg agccatccgg atggccgggc cgtgtcccgg ccgctcacga ggcaggagag
     1021 gagcaggcgg tggagccgac gcagacggaa gtcccgaacg ccgtggaggg ctggcagccg
     1081 acgctcgacc cggtgacgtt cgaggtcctg aagaactcct tctcgacgat cgtcgaccag
     1141 atggcggagc agatccttcg cacctgttac tcgttcgtga tctacgcgaa ggacttctcg
     1201 tccgccctga ccgaccacga gggcaacacg gtcatgcagg gcagccacga cgtcgccgtc
     1261 cacgtcggca ccctgcactt cagcgccaag gcggtgctcg agatcttcgg cgacgagatg
     1321 gcgccgggcg acgtctacgc gatgaacgac ccctacctcg gcggcacgca cttcaacgac
     1381 gtgcggctga tccggcccgt cttcgtcgac ggcgagctga tcgcgatggt ccagtgcaac
     1441 ggccactggt ccgacgtcgg cggctcggtc ccgggctcgc acgacatcac cgcccgcgag
     1501 cacttccagg agggcctgcg gatcacgccg atgaagatct gggacaaggg cgtcttccgt
     1561 gacgacgtcg tgcggatgat cgtctcgaac acgcgcacgc cggtcgactg cgagggcgac
     1621 ctgcacgccc aggccgaggc gacccgcgtc gccgagagcg agatcctgcg gctcgtcgag
     1681 aagtacggca aggacaccgt caagaccgcc ttcgccgacg tccagaacta cgtcgagacg
     1741 atcatgcgcg gccgtctcgc cggcctgccc gacggcagct gggaggccga ggactacatc
     1801 gacttcgacc cggccgccgg cgaggagggg ctgatcccgg tcaaggtcaa gatgacgatc
     1861 gacggcaacg acctgcgcta cgacctgacc ggctcgcacc cggtcatcgg cagcgcgctg
     1921 aactgcgcgt tcggcatggg cttctccggc gtcgtcgtcg gcacgaagac gttcttcccc
     1981 gacatcccgc tgaactccgg cttctaccgc gcgatggacg tcgacctcgg gccggtcggc
     2041 agcgtcgtca acgcgccaca cccggtggcg gtgaccggct tcaacgccgg cgcctacgag
     2101 aagatcgtca acgcgatctt cgagctctgg tcgggcctga tgcccgagcg cgcgctcgcc
     2161 tgctcctaca acctcgagta cctgctcgtc ggcggccgcg acggccgcga tcccgagcgc
     2221 aacatcttca tgtggtacga ctggatggcc ggcggctggg gcgggcgcaa cggcaaggac
     2281 gggcttaacg gcacctcgcc gatcttcggc gccgggctcg aggtccagcc gctcgagggc
     2341 caggagcggc tctcgccggt gctcaccacg cgccacgagt tcatccccga ctccggcggg
     2401 ccgggccgct tccgcggcgg cgtcggcgtc gagaagggcg ccatcctcca gcaggccgag
     2461 agcacggtga tgtcctactc gtgcgaccgc gcccgcagca tcacctgggg catcggcggc
     2521 ggcctgccgt cgaacccgca cggcgtctgg ctcgaccccg acggaccgga gcgccactac
     2581 ctcggcgcct ggttcgcgag catgccggcg gcgaagggaa cgaccttcac ccgcccgtcg
     2641 gcgggcggtg gcggctatgg cgacccgctc gagcgcgatc cgaacgcggt ccgcgaggac
     2701 gtcgccgacg gctacgtgac gatcgagcgc gccgccaagg actacggcgt cgtgatccgg
     2761 acggtcgacg aggacctcgc cgcctacgag gtcgacgagg ccgcgacgct cgccgagcgc
     2821 gagcggatcg ccgccaaccg cctcgggtgg atcgaggagg acccggagag cgtcgccgag
     2881 cgctaccgcg ccggcgagct cgacgtcttc gactgcgtcc gccagcacgg ggtcgtgctc
     2941 gactggggga ccggcgagct gctgcgcaac acgaccgagc agttccgtgc gatgctcgcg
     3001 aggcgaacga ccgccttctg ggacgcgccg gcggcggccg gcgcggcctg aggcgagggt
     3061 cgcgaggccg aggcgcaccg gcgatgccgc tgccccccga acgctgcgac gtggccgtgg
     3121 tcggcgccgg catcgtcggc ctctcggtcg cccgcgagct gacgcgccgc gaccccggcg
     3181 cgagcgtcgc cgtgatcgag cgcgagcggc gcctcgccgc ccaccagacc tcgcacaact
     3241 ccggcgtcgt gcacgccggc atctactacc gcccggggtc gctgaaggcg aagctctgcg
     3301 tcacgggcgc gcgcgagctg cgcgagtact gcgagcggcg cgcgatcccc taccgcgagg
     3361 tcggcaaggt gatcgtcgcg ctcgacgagt ccgagctgcc ggcgctcgac ggcctccagg
     3421 cccgcgccgt cgccaacggc gtgccgcgcg tcgagcggat cggccgcgag cggctgcgcg
     3481 agctcgagcc cgcggtccgc ggcatcgccg cactgcacag cccagcgacg g
//
LOCUS       DATABV010000008        11932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_83533, whole genome shotgun sequence.
ACCESSION   DATABV010000008 DATABV010000000
VERSION     DATABV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83533"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /gene="dnaE"
                     /locus_tag="VHF88_00145"
     CDS             complement(<1..1688)
                     /gene="dnaE"
                     /locus_tag="VHF88_00145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2160216.1"
                     /translation="MTCAHLHVHSEYSLLDGAAKIDKLAERAAAYGQPALGLTDHGVM
                     NGAVEHYKACRKHGIKPIIGLEAYYVDDRRTEAVRYERNHLTLLAADDAGFRNLVKLT
                     SAGFLEGYKRGKANVDMELLDRHSEGVIALTGCLASRFCQRLIADNPAEARAHTDELM
                     QVFGRDNVYFEVQKNGIAEQDKANEGIVAIAREVGRPLVATGDVHYLGREDYENHSAL
                     LCVQTKSTLAEPKLTFDTNEFYLKDSAEMADQFAVWPEAVPTTIEIADRCEIDIELGK
                     MLIPSYPTPTGEVEGAYLRRLAEEGLRRRYGDPVPAEATERLDMELEVIGRMGFDAYF
                     LIVWDFVKYAKDSGIAVGPGRGSAAGSIVSYCLDITEVDPLAYGLLFERFLNPERVSM
                     PDIDIDFSVKGREQVMRYVADKYGRESVAQIVTFGKMLPRNATRDAARVLGLDYGTGD
                     RLAKLIPEPVMGRSKSFDEYLKEEPELKRAYDQEPDARRVIDTAKGLEGIVRNAGIHA
                     AAVVIADRPLTDIVPLQLMEDRGASVDGERAYRTVTQYSMKPIEEIGLLKMDFLG"
     gene            complement(1779..1940)
                     /locus_tag="VHF88_00150"
     CDS             complement(1779..1940)
                     /locus_tag="VHF88_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160217.1"
                     /translation="MSSKSYTALGWIVWQIGSRLAKRKLARNRSKLLAAGVVAAVVAA
                     GIALASNDD"
     gene            complement(1937..3217)
                     /locus_tag="VHF88_00155"
     CDS             complement(1937..3217)
                     /locus_tag="VHF88_00155"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX2160218.1"
                     /translation="MSEPGTATPLGSYEAGPGEVGRVLLLYSGGLDTSVMLKWIQDHY
                     GAEVVALTLNLGQPGEDYDVVRGKALDLGALACEIVDAREEFARDYVLPAIKANALYG
                     GGYPLFTALGRPLIAKIAVEKAREHGCDAVAHGCTGKGNDQVRLDSAVLAYAPEMKIV
                     APVRNWQMGREEEIAYAREHGIPVKGGTEAPPYSIDDNIWGRSSEGGPIEDLGEPPRD
                     DVFELVADVRQAPDEPEDLVVGFERGCPVSLNGEALGLVELIDRAAEIAGRHGVGTVD
                     HIEDRIVGLKVRDLYEVPAAEVILTAHRELEKLVGTIHQNRFKRSLDEQWAFLVYAGL
                     WYEPLREDLDAYMDSVNEHVTGEVTVRLHKGRAQAVARSSPNALYDEALAGFGESGGL
                     FSQHASPGFIELWTLQSRMAHGIRNRARAGKDPA"
     gene            complement(3288..4433)
                     /locus_tag="VHF88_00160"
     CDS             complement(3288..4433)
                     /locus_tag="VHF88_00160"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HEX2160219.1"
                     /translation="MSLADLQRIEARAVIGTYARNPVEFVRGEGTRLWDDEGNEYLDF
                     LAGISVVQIGHCHPCLVEAVSDQAARLMHVGNLFYTEPGIRLAERLSDRGLGGKAFLC
                     NSGAEAIECALKLARRHRPGGRFVVMEGGFHGRTMGALSATPQEAKQAPFAPLVPGFE
                     VVGRNEAAALEAAVDGDTAAVLLEPIQGESGIHPLSEEALSAARAACDRHGALLIFDE
                     IQCGMGRTGDLWAWQGAGVRPDVMTVAKGLGGGLPIGACVTGPNHADVLQRGDHGSTF
                     AGGPAIAAGAHAVLDVVDDRAFLTDVAHKGEHLLGALARLGLEVRGRGLMVGFRCDDA
                     PALARRLLLDHRLVVNATGPDTIRLLPPLTVSEAEIDEAIARIGKAL"
     gene            complement(4441..5280)
                     /gene="argB"
                     /locus_tag="VHF88_00165"
     CDS             complement(4441..5280)
                     /gene="argB"
                     /locus_tag="VHF88_00165"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX2160220.1"
                     /translation="MRDVATLLEALPYIRDFHGRTVVIKYGGAAMTDDALKEDFARDV
                     VLLKYVGMNPVVVHGGGPDITAYMERLGMKVEFVDGLRVSDEATVEVAKMVLVGKQNK
                     DIVLRLGRHGQPAVGLCGDDGRLFTVRKQLAGEVDIGFVGEIEHVDAGVLHHVAADYI
                     PVIASVGADAVGNSYNVNADTAAAAVAAALGASKAIFLTDVVGWLADPADEGSLIAQA
                     DLADVKQRLAGIQGGMRPKLEACVAALEGGVPNAHIIDGRRPHSLLLELFTDKGIGTK
                     LFR"
     gene            complement(5280..6542)
                     /gene="argJ"
                     /locus_tag="VHF88_00170"
     CDS             complement(5280..6542)
                     /gene="argJ"
                     /locus_tag="VHF88_00170"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX2160221.1"
                     /translation="MSGFFRSRWVDRPDHVTELDPHALPAGFRAAGVHAGIRGKALGD
                     APPPLDVGLLVSDREATVSAVRFCDSALIGAPVAVTSESRTDGLRAVLANSGNANVAD
                     GARGLETARTAQAAAAEALGIDAELVGLASTGVIGRELPLEALTAGARACADALGDDS
                     ADFCQAILTTDRWPKRACLGVELPSGGTVRLAGQVKGAGMISPRFATMFCFVETDAEL
                     EQATLDLLTGVCVKRSFDRVSVDGQLSTSDTVIAFANGAAGVRVEPESPDELALGQAM
                     DALMRQLALEMVADGEGATRVGRVVVRGAEELVEPVARGVADSPLVKSALLGADPNFG
                     RILQSVGQVLGGGAPFVVDLAIEGVQVASAGVVLDLDAETWRTLERAVESREVEIDLT
                     LPGEGGETELFFSDLTHQYVTINSEYTT"
     gene            complement(6539..7525)
                     /gene="argC"
                     /locus_tag="VHF88_00175"
     CDS             complement(6539..7525)
                     /gene="argC"
                     /locus_tag="VHF88_00175"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX2160222.1"
                     /translation="MAEHRVAVVGAAGFGGAVAASILDRHPSLELTALTARTDAGKRL
                     CDVYPRHRVGMTMEEYDADRVAEAADAVVLGYPHKAAAPVVRELRERGVKVVDMSADF
                     RLSRELYEREYQPHEAPELLGDAVYGLPELGHREAIAGAELVGSPGCYPTASLLGLWP
                     LRAVAADAIVDAKSGVSGAGREATATTHFVSADENVTPYKVEGHRHRAELDQELPGLT
                     VTFTPHLLPIDQGLMASCYVTPRSTLTGDEVRELFLDAYGDEPFVELADAPPGVRDVR
                     ETNLCRIHATVEPATGRVLVFAAIDNLWKGAAGQAVQCLNLMLGLPETEGLL"
     gene            complement(7518..10076)
                     /gene="pheT"
                     /locus_tag="VHF88_00180"
     CDS             complement(7518..10076)
                     /gene="pheT"
                     /locus_tag="VHF88_00180"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2160223.1"
                     /translation="MRVPLTWLREYCHPGLGAVEIAERLDLTGTELERIEQVGVPSAD
                     GFVVGRVLSAEQHPDADRLKVCRVDVGAGEPSTIVCGAPNVAAGQTVPVALPGATMPG
                     GTKLGKARLRGVESHGMILAEDELGIGDQHSGIVVLEDGLAPGAALADIVPIADEVLE
                     LEITPNRPDCLSVYGIAREVHAATGVPLAGDPTDADTEGSGSDDVSGHATVEIVDPDV
                     CLRFTARVFEGVTIRPSPLWLKQRLIAAGQRPINNVVDITNYVMLLSGQPMHAFDLDE
                     VRGSRIVVRRARSGETMTTLDGVERTFDASMALVCDVEGPSGIAGIMGGQVSEVSETT
                     TRVLMEAATWVGPNVLKTSKALGLRSEASTRFEKQLHPEQALAAQRLAARLMVELAGA
                     RLVPGTIDAYPAPVEPRVVTLGFDRLERMLGVRIDDGEVERILASLGFDCERDSGSGG
                     TGGAALAVTVPPWRDADVQREADLVEEVARVYGLDKLPTTLPARERAVGRLTHAQRLR
                     RRLEDALRDRGMDEVVAWSFTSPETLAKLRVGDGEVLELANPLSEDHSVMRPLLLPGL
                     LDAARRNAAHGAAGVALFESAHVYAAGGGLEAPEGSPKGATPAVERHHLAGLITESSP
                     GTWRSGAGAADVYAAKGFVEALLKVAGLSLVVEPAKDVPFLHPGRAGSAFVAAGPVDG
                     EISGAQKGCAPEDFPAPPAEAASGANGSGMVPIGWIGELHPLVAREWDLEGGAAFELD
                     ADLLAELTEGRLATYQDVTSFPAVLQDIAVVAAVDVSAAEVEAAVRQGGGELLASVRV
                     FDVYEGEQVGEGKRSLALRLEFRAADRTLTEDEVVERRTAIEAALGEIGGRIRG"
     gene            complement(10073..11137)
                     /gene="pheS"
                     /locus_tag="VHF88_00185"
     CDS             complement(10073..11137)
                     /gene="pheS"
                     /locus_tag="VHF88_00185"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX2160224.1"
                     /translation="MPDAPARLQEIRAEAEAAIAAAGSAAELEQLRIQYLGRKAELTQ
                     MLRSIKDLPPEQRGPVGKGGNEVKQALEAQLEQRVAALDAAELDDRLRSDAIDVTLPG
                     DPPRRAGHLHLITETRREIEDVFLGLGFAVAEGPEVEYDYYNFTALNHPPDHPARMLQ
                     DTFYFSGDVLLRTHTSPMQVRAMELQEPPIYIVVPGKVYRRDHDATHTPMFHQIEGLA
                     IDTDITLADLQGVLLEFVRAIFGADREIRLRPHYFPFTEPSVEVDVSCFKCSGKGYLA
                     DGSHCNLCRGSGWIEILGSGMVDPNVLGFVADNGYDPSKVQGFAFGMGIERIAMLRHG
                     VPDLRLFFDNDVRFLEQFGA"
     gene            complement(11130..11831)
                     /locus_tag="VHF88_00190"
     CDS             complement(11130..11831)
                     /locus_tag="VHF88_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2160225.1"
                     /translation="MITSRDNSQLKQIHKLQRKKERDATGLFAAEGEDLIAAAEAAGW
                     TPEILLRAGEDVEPELLERVSSLGSGARVIGVYRQRWSEPRGDVCVYLHGVGDPGNVG
                     TVVRAAHALCDGPVVLGPGCADPYGPKAVRASMGSLFGRPPGRAALSDLPGTKLALDG
                     GAERTLADAVAELSAPVVLCVGGERDGLPADLLATADVVARIPQRADGPDSLNVAMAA
                     TVGLYELGNRMTAHA"
BASE COUNT         1726 a         4478 c         4000 g         1728 t
ORIGIN      
        1 ccgaggaagt ccatcttcag caggccgatc tcctcgatcg gcttcatcga gtactgcgtg
       61 acggtgcggt aggcgcgctc gccgtcgacc gaggcgccgc gatcctccat cagctgcagc
      121 gggacgatgt cggtcagcgg acggtcggcg atcacgacgg cggcggcgtg gatgccggcg
      181 ttgcggacga tcccctcgag gcccttggcg gtgtcgatca cgcgccgcgc gtccggctcc
      241 tggtcgtagg cgcgcttgag ctccggctcc tccttgaggt actcgtcgaa gctcttcgag
      301 cggcccatca cgggctccgg gatcagcttc gcgagccggt cgccggtgcc gtagtcgagg
      361 ccgagcacgc gcgccgcgtc gcgcgtcgcg ttgcgcggca gcatcttgcc gaaggtgacg
      421 atctgggcga ccgactcgcg gccgtacttg tcggcgacgt agcgcatcac ctgctcgcgg
      481 cccttgaccg agaagtcgat gtcgatgtcg ggcatcgaca cgcgctcggg gttgaggaag
      541 cgctcgaaca gcagcccgta ggcaagtggg tcgacctcgg tgatgtccag gcagtaggag
      601 acgatcgagc cggccgccga gccacggccc gggccgaccg ctatgccgga gtccttggcg
      661 tacttgacga agtcccagac gatcaggaag taggcgtcga agcccatccg gccgatcacc
      721 tcgagctcca tgtcgaggcg ctcggtggcc tcggcgggaa ccgggtcgcc gtagcggcgg
      781 cgcagcccct cctccgccag ccgccgcagg tacgcgccct cgacctcgcc ggtcggcgtc
      841 gggtagctcg ggatcagcat cttgccgagc tcgatgtcga tctcgcagcg atcggcgatc
      901 tcgatcgtcg tcggcaccgc ctcgggccag acggcgaact gatcggccat ctcggccgag
      961 tccttcaagt agaactcgtt ggtgtcgaag gtcagcttcg gctcggcgag cgtgctcttg
     1021 gtctggacgc agagcagcgc ggagtggttc tcgtagtcct cgcggccgag gtagtggacg
     1081 tcgccggtcg cgaccagcgg ccggccgacc tcgcgggcga tcgcgacgat cccctcgttg
     1141 gccttgtcct gctcggcgat gccgttcttc tggacctcga agtagacgtt gtcgcgcccg
     1201 aagacctgca tcagctcgtc ggtgtgggcg cgcgcctcgg ccgggttgtc ggcgatcagc
     1261 cgctggcaga agcgcgacgc caggcagccg gtgagcgcga tcacgccctc ggagtggcgg
     1321 tcgagcagct ccatgtcgac gttcgccttg ccgcgcttgt agccctcgag gaagccggct
     1381 gacgtcagct tgacgaggtt gcggaagccg gcgtcgtcgg cggcgagcag cgtcaggtgg
     1441 ttgcgctcgt agcggaccgc ctcggtcctg cggtcgtcga cgtagtaggc ctcgaggccg
     1501 attatcggct tgatcccgtg cttgcggcag gccttgtagt gctcgaccgc gccgttcatg
     1561 acgccgtggt cggtcagccc gagcgccggc tgcccgtagg ccgccgcccg ctcggcgagc
     1621 ttgtcgatct tcgcggcgcc gtcgagcagc gagtactcgg agtggacgtg gaggtgggcg
     1681 caggtcacgg gccgccgaat atacgcatgg cgcaagacgg ctccgaaccg ttacgagaca
     1741 gcccccgctc tttgcggaaa cgttgcgaag acgtgttatt agtcgtcgtt ggaggcgagg
     1801 gcgatgcccg cggctacgac ggcggctact acgccggcgg cgaggagctt ggagcggttg
     1861 cgggcgagct tgcgcttggc gaggcgggag ccgatctgcc agacgatcca gccgagggcg
     1921 gtgtagctct tggagctcat gccgggtcct tacccgcccg cgcgcggttc cttatcccgt
     1981 gggccatccg cgactggagc gtccagagct cgatgaagcc ggggctggcg tgctgggaga
     2041 agaggccgcc ggactcgccg aagccggcga gggcctcgtc gtagagagcg ttgggcgagc
     2101 tgcgcgcgac ggcctgggcg cggcccttgt ggagtcgcac ggtcacctct ccggtgacgt
     2161 gctcgttgac ggagtccatg taggcgtcga ggtcctcgcg cagcggctcg taccagaggc
     2221 cggcgtagac gaggaaggcc cactgctcgt cgagcgagcg cttgaagcgg ttctggtgga
     2281 tcgtgccgac gagcttctcg agctcgcggt gcgcggtcag gatgacctcg gcggccggca
     2341 cctcgtagag gtcgcgcacc ttgaggccga cgatccggtc ctcgatgtgg tcgaccgtgc
     2401 cgacgccgtg acggccggcg atctccgctg cgcgatcgat cagctccacg aggccgagcg
     2461 cctcgccgtt cagcgacacc gggcagccgc gctcgaagcc gacgaccagg tcctccggct
     2521 cgtcgggcgc ctggcggacg tccgcgacca gctcgaagac gtcgtcgcgt ggcggctcgc
     2581 cgaggtcctc gatcgggccg ccctcggacg agcggcccca gatgttgtcg tcgatcgagt
     2641 aaggcggagc ctcggtcccg cccttgaccg ggatcccgtg ctcgcgcgcg taggcgatct
     2701 cctcctcgcg ccccatctgc cagttgcgca ccggtgcgac gatcttcatc tccggcgcgt
     2761 aggcgagcac cgccgagtcg agccgcacct ggtcgttgcc cttgccggtg cagccgtggg
     2821 cgacggcgtc gcagccgtgc tcgcgcgctt tctcgacggc gatcttcgcg atcagcggcc
     2881 ggccgagcgc ggtgaagagc gggtagccgc cgccgtagag ggcgttcgcc ttgatcgccg
     2941 gcagcacgta gtcgcgtgcg aactcctcgc gcgcgtcgac gatctcgcag gccagtgcgc
     3001 cgaggtcgag cgccttgccg cgcacgacgt cgtagtcctc gccgggctgc ccgaggttga
     3061 gcgtcagcgc gaccacctcg gcgccgtagt ggtcctggat ccacttcagc atcaccgacg
     3121 tgtcgaggcc gcccgagtag agcagcagca cgcggccgac ctcgccggga ccggcctcgt
     3181 aggagcccaa cggcgttgct gtccccggct cgctcatcgg cggggcaacc taccagcgac
     3241 cggccaggcg ccacgggggg ccggggggcg cgagcccccc gatgtcatca cagggccttg
     3301 ccgatcctgg cgatcgcctc gtcgatctcg gcctccgaga cggtcagcgg cggcagcagg
     3361 cggatcgtgt ccggcccggt ggcgttgacc accaggcggt ggtccagcag caggcgacgg
     3421 gccagcgcgg gagcgtcgtc gcagcggaag ccgaccatca gcccgcggcc gcgcacctcg
     3481 agcccgaggc gggcgagcgc gccgagcagg tgctcgccct tgtgggcgac gtccgtcagg
     3541 aacgcacggt cgtcgacgac gtcgagcacc gcgtgggcgc ccgcggcgat cgcggggccg
     3601 ccggcgaacg tcgatccgtg gtcgccgcgc tgaagcacgt cggcatggtt cgggccggtc
     3661 acgcaggcgc cgatcggcag gccgcctccc agccccttgg cgacggtcat cacatctggc
     3721 cgcacgccgg cgccctgcca ggcccagagg tcgccggtgc gtcccatccc gcactggatc
     3781 tcgtcgaaga tcagcagcgc cccgtggcgg tcgcaggcgg cgcgcgcggc gctcagcgcc
     3841 tcctcggaca gcgggtggat gccggactcg ccctggatcg gctcgagcag caccgcggcg
     3901 gtgtcgccat cgacggcggc ctcgagcgcg gccgcctcgt tgcgaccgac gacctcgaag
     3961 cccgggacca gcggcgcgaa cggcgcctgc ttcgcctcct gcggcgtcgc ggagagggcg
     4021 cccatcgtgc gcccgtggaa gccgccctcc atcacgacga agcggccgcc cggccggtgg
     4081 cggcgcgcga gcttgagcgc gcactcgatc gcctcggcgc ccgagttgca gaggaacgcc
     4141 tttccaccga gcccgcggtc ggaaagccgc tcggcgagac ggatgcccgg ctcggtgtag
     4201 aagaggttgc cgacgtgcat caggcgggcg gcctggtcgg agaccgcctc gaccaggcac
     4261 ggatgacagt ggccgatctg gacgaccgag atgccggcga ggaagtcgag gtactcgttg
     4321 ccctcgtcgt cccagagccg ggtgccctcg ccgcggacga actcgaccgg attgcgcgcg
     4381 taggtgccga tgaccgctcg ggcctcgatc cgctggaggt cggcgaggct cacggcacga
     4441 tcaccggaac agcttggtgc cgatgccctt gtcggtgaac agctcgagca gcagcgagtg
     4501 cggccgccgc ccgtcgatga tgtgcgcgtt cggcacgccg ccctcgagcg ccgcgacgca
     4561 agcctcgagc ttcggccgca tcccgccctg gatgccggca agccgctgct tgacgtcggc
     4621 gaggtccgcc tgggcgatca gcgagccctc gtcggcgggg tcggcgagcc agccgacgac
     4681 gtcggtcagg aagatcgcct tcgaggcgcc gagggccgcc gcgacggcgg ccgctgcggt
     4741 gtcggcgttg acgttgtacg agttgcccac ggcgtccgcg ccgaccgagg cgatcacggg
     4801 gatgtagtcg gcggcgacgt ggtgcagcac tccagcgtcg acgtgctcga tctcgccgac
     4861 gaagccgatg tcgacctctc cggcgagctg cttgcgaacg gtgaagagcc gtccgtcgtc
     4921 gccgcagagc ccgaccgccg gctgcccgtg acgccccagg cgcagcacga tgtccttgtt
     4981 ctgcttgccg acgaggacca tcttcgccac ctcgaccgtc gcttcgtcgg agacgcgcag
     5041 gccgtcgacg aactcgacct tcatgccgag ccgctccatg taggccgtga tgtcgggccc
     5101 gccgccgtgg acgacgaccg ggttcatccc gacgtacttg agcagcacga cgtcgcgcgc
     5161 gaagtcctcc ttgagcgcgt cgtcggtcat cgccgcgccg ccgtacttga tcaccaccgt
     5221 ccgcccgtga aagtcgcgga tgtagggcag ggcctcgagc agtgtcgcga cgtcacgcat
     5281 cacgtcgtgt actcgctgtt gatcgtcacg tactggtggg tgagatcact gaagaacagc
     5341 tcggtctcgc cgccctcgcc gggcagcgtc aggtcgatct ccacctcgcg ggattcgacc
     5401 gcgcgctcga gcgtcctcca cgtctcggcg tcgagatcga gcacgacgcc cgccgaggcg
     5461 acctgcacgc cctcgatcgc gaggtcgacg acgaacggcg cgccgccacc cagcacctgt
     5521 ccgaccgact gcaggatccg accgaagttc gggtcggcgc cgagcagcgc cgacttgacc
     5581 agcggcgagt cggcgacccc gcgcgcgacc ggctcgacga gctcctcggc gccgcgcacg
     5641 acgacccgcc cgacccgcgt cgcgccctcg ccgtcggcca ccatctcgag cgcgagctgg
     5701 cgcatcagcg cgtccatcgc ctggccgagc gccagctcgt cgggcgactc gggttcgacc
     5761 cgaacgccgg cggccccatt ggcgaacgcg atcacggtgt ctgaggtcga cagctggccg
     5821 tcgaccgaga cacggtcgaa cgaccgcttg acgcagacgc cggtcagcag gtcgagcgtc
     5881 gcctgctcga gctcagcgtc ggtctcgacg aagcagaaca tcgtcgcgaa gcgcggcgag
     5941 atcatccccg cacccttgac ctgaccggcc agccggaccg tgccgcccga gggcagctcg
     6001 accccgaggc aggcccgctt cggccacctg tcggtcgtca ggatcgcctg gcagaagtcg
     6061 gcgctgtcgt cgccgagcgc gtcggcgcag gcccgcgccc ccgcggtcag cgcctcgagc
     6121 ggcagctcgc ggccgatcac gcccgtcgag gccaggccga ccagctccgc atcgatcccc
     6181 agcgcctcgg cggcagccgc ctgcgccgtc cgcgccgtct cgagtccgcg cgcgccgtcg
     6241 gcgacgttcg cgttgcccga gttcgcgagc accgcccgca gtccgtcggt ccgcgactcg
     6301 ctggtcaccg cgaccggcgc cccgatcagc gcgctgtcgc agaagcgaac cgccgacacc
     6361 gtcgcctcgc gatcggagac cagcaacccg acgtcgagcg gcggcggcgc gtcgcccagc
     6421 gccttgcccc tgatgcccgc gtggacgccc gccgcgcgaa agcccgccgg cagggcgtgt
     6481 gggtcgagct cggtcacatg atcgggccgg tcgacccagc gcgaccggaa gaacccgctc
     6541 acagcagacc ctccgtctcc ggcaggccca gcatcaggtt caggcactgc accgcctgcc
     6601 ccgccgctcc tttccagagg ttgtcgatcg ccgcgaagac cagcacccgg cccgtagccg
     6661 gctcgaccgt cgcatggatc cggcagaggt tcgtctcgcg cacgtcgcgc acgcccggcg
     6721 gcgcgtccgc cagctccacg aacggctcgt cgccataggc atccaggaac agctcgcgca
     6781 cctcgtcgcc ggtcaaggtc gaccgtggcg tcacgtagca gctcgccatc aacccctggt
     6841 cgatcggcag cagatgcggc gtgaacgtca ccgtcagacc cggcagctcc tggtccagct
     6901 ccgcccgatg ccgatgcccc tcgaccttgt aaggcgtcac gttctcgtca gcggacacga
     6961 agtgagtagt ggccgtcgcc tcccgcccag cccccgacac acccgacttc gcatcgacga
     7021 tcgcatccgc cgcgaccgcc cgcaacggcc acagccccag cagcgacgcc gtcggatagc
     7081 agcccggcga ccccaccagc tccgcgcccg cgatcgcctc tcggtgcccc agctccggca
     7141 gcccgtagac cgcgtctccc aacagctccg gcgcctcatg cggttggtac tcccgctcat
     7201 acagctcccg cgacagccgg aagtcagccg acatgtcgac caccttcacc ccccgctccc
     7261 tcagctcccg caccaccggc gccgccgcct tgtgcggata ccccaacacc accgcatccg
     7321 ccgcctcagc gacccgatcc gcgtcgtact cctccatcgt catccccacc cgatgccgcg
     7381 gatacacgtc acacagccgc ttccccgcat ccgtccgcgc ggtcaaagcc gtcaactcca
     7441 gcgacggatg ccgatccaag atcgacgccg cgaccgcccc cccaaaccca gccgcgccca
     7501 ccaccgcaac ccgatgctca gccacggatc cgccccccga tctccccgag cgccgcctcg
     7561 atcgccgtcc gccgctcgac gacctcatcc tcggtcaacg tccgatccgc cgcccggaac
     7621 tccagtcgca gcgccagcga ccgcttgccc tcacccacct gctccccctc atacacgtcg
     7681 aagacgcgca cagacgccaa cagctcccca ccgccctgcc gcaccgccgc ctcgacctcc
     7741 gcagcgctca cgtccacagc cgccacgacc gcaatgtcct gcagcacagc agggaagctc
     7801 gtcacgtcct gataggtcgc aagcctcccc tccgtcagct cggccagcaa atccgcatcc
     7861 aactcgaagg cggcaccccc ttccaggtcc cactcgcgcg cgaccagcgg gtgcaactcc
     7921 ccgatccaac cgatcggcac catcccagac ccgttggccc ccgacgccgc ttccgcgggt
     7981 ggcgcgggga aatcctcggg cgcgcagccc ttttgagcac ccgagatttc cccgtcgaca
     8041 ggacccgcgg ccacgaacgc cgatcccgca cgcccggggt gaagaaacgg cacatcctta
     8101 gccggctcga cgaccaacga cagccctgcc accttcagca gcgcctccac gaaccccttc
     8161 gccgcataga cgtccgccgc ccccgccccg ctccgccacg tccccggcga cgactccgtg
     8221 atcagccccg caaggtgatg ccgctcgact gcaggcgtcg cacccttggg actcccctcc
     8281 ggcgcctcca gcccgccgcc cgccgcatac acatgcgccg actcgaacaa cgccacccca
     8341 gccgccccat gcgccgcatt ccgccgcgcc gcatccaaca gccccggcaa cagcagcggc
     8401 cgcatcaccg agtggtcctc gctcaacggg ttcgccagct ccagcacctc accatcgccc
     8461 acccgcagct tcgccagcgt ctccggcgac gtgaagctcc acgccacgac ctcgtccatc
     8521 ccccggtcgc gcagcgcatc ctccaaccgc cgccgcaacc gctgagcatg ggtcagccgg
     8581 cccactgccc gctcgcgtgc gggcagcgtc gtgggcagct tgtcgagccc gtagacccgc
     8641 gcgacctcct cgaccaggtc cgcctcccgc tggacgtccg cgtctcgcca cggcggcacg
     8701 gtcaccgcga gggcagcgcc gcccgttccc cccgagccgc tgtcgcgctc gcagtcgaag
     8761 ccgaggctcg cgaggatccg ctcgacctcg ccgtcgtcga tccgcacgcc gagcatccgc
     8821 tccagccggt cgaagccgag cgtcacgacg cgcggctcca ccggcgccgg gtaggcgtcg
     8881 atcgtcccgg ggaccaaccg cgcgcccgca agctcgacca tcagccgcgc cgcgagccgc
     8941 tgtgccgcca gcgcctgctc gggatgaagc tgcttctcga accgcgtcga cgcctcggag
     9001 cgcaggccga gcgccttcga ggtcttgagc acgttcgggc cgacccaggt cgcggcctcc
     9061 atcaggaccc gtgtcgtggt ctcggagacc tcggagacct ggccccccat gatcccggcg
     9121 attcccgacg gcccctcgac gtcgcagacc agcgccatcg acgcgtcgaa cgtccgctcg
     9181 accccgtcga gggtcgtcat cgtctccccg ctgcgcgccc ggcgcacgac gatccgcgat
     9241 ccccgcacct cgtcgaggtc gaaggcgtgc atcggctggc cgctgagcag catcacgtag
     9301 ttggtgatgt cgacgacgtt gttgatcggc cgctggccgg ccgcgatcag ccgctgcttg
     9361 agccacagcg gcgacggccg gatcgtcacg ccctcgaaga cgcgcgccgt gaagcgcagg
     9421 cagacatccg gatcgacgat ctcgaccgtg gcgtggccgg agacgtcatc tgagccgctt
     9481 ccctcggtgt ccgcgtcggt cggatcgccg gctagcggca cgccggtggc ggcgtggacc
     9541 tcgcgcgcga tcccgtagac cgacaggcag tccggccggt tcggcgttat ctcgagctcg
     9601 agcacctcgt cggcgatcgg gacgatgtcc gcgagcgcgg cccccggcgc gagcccgtcc
     9661 tcgagcacga cgatcccgga gtgctggtcg ccgattccga gctcgtcctc ggcgaggatc
     9721 atcccgtgcg actcgacgcc gcgcagcctg gccttgccca gcttcgtgcc gccgggcatc
     9781 gtcgcgccgg gcagcgcgac gggaacggtc tgcccggccg cgacgttcgg cgcgccgcag
     9841 acgatcgtgc tcggctcgcc ggcgccgacg tcgacccggc agaccttgag ccggtcggcg
     9901 tcggggtgct gctccgccga cagcacccgg ccgacgacga acccgtcggc cgacggcacg
     9961 ccgacctgct cgatccgctc gagctcggtg ccggtcaggt cgagccgctc ggcgatctcg
    10021 accgcgccga ggccggggtg gcagtactcg cgcagccatg tcaacgggac cctcatgcgc
    10081 cgaactgctc caggaagcgg acgtcgttgt cgaagaacag ccgtaggtcc ggcacgccgt
    10141 ggcgcagcat cgcgatccgc tcgatgccca tgccgaaggc gaagccctgc accttcgacg
    10201 ggtcgtagcc gttgtcggct acgaagccga gcacgttggg gtcgaccatc cccgagccga
    10261 ggatctcgat ccagccgctc ccgcggcaca gattgcagtg ggacccatcc gcgaggtatc
    10321 ccttgccgct gcacttgaag cacgagacgt cgacctcgac gctcggctcg gtgaacggga
    10381 aatagtgcgg ccgcagccgg atctcccggt cggcgccgaa gatcgcgcgg acgaactcga
    10441 gcagcacgcc ctggaggtcg gccagcgtga tgtcggtgtc gatcgccagc ccctcgatct
    10501 ggtggaacat cggcgtgtgg gtggcgtcgt ggtcgcgccg gtacaccttg ccggggacga
    10561 cgatgtagat cggcggctcc tgcagctcca tcgcgcgcac ctgcatcggc gacgtgtgcg
    10621 tccgcagcag gacgtcgccg gagaagtaga aggtgtcctg gagcatccgc gccggatgat
    10681 cgggcgggtg gttgagcgcg gtgaagttgt agtagtcgta ctcgacctcg ggaccctcgg
    10741 cgacggcgaa gccgagcccg aggaagacgt cctcgatctc gcgccgcgtc tcggtgatca
    10801 ggtgaaggtg gccggcgcgg cgcggcgggt cgccgggcag cgtgacgtcg atcgcgtcgg
    10861 agcgcagccg gtcgtcgagc tcggccgcgt cgagcgcggc cacacgctgc tcgagctggg
    10921 cctcgagcgc ctgcttgacc tcgttgccgc ctttgccgac ggggccgcgc tgctccggcg
    10981 gcaggtcctt gatcgagcgc agcatctgcg tcagctcggc cttgcgcccg aggtattgga
    11041 tccgcagctg ctcgagctcg gccgccgatc ctgccgcggc gatcgccgcc tctgcctcgg
    11101 cgcggatctc ctgcaggcgg gccggggcgt caggcatgag cggtcatcct attcccgagc
    11161 tcgtagaggc ccacggtcgc ggccatcgcg acgttgagcg aatccggccc gtcggcccgc
    11221 tggggaatcc gcgcgacgac gtccgcagtc gccagcaggt cggcgggcag tccgtcgcgc
    11281 tcgccgccga cgcacaggac gactggcgcc gagagttcgg cgaccgcgtc ggcgagcgtc
    11341 cgctcggcgc cgccgtcgag cgcgagcttc gttcctggca ggtcggacag cgccgcccgc
    11401 ccgggcggcc gaccgaacag cgagcccatg ctcgcgcgca ccgccttcgg cccgtagggg
    11461 tcggcgcagc cggggcccag caccaccggt ccgtcgcaga gcgcgtgcgc cgcccgcaca
    11521 accgtcccga cgttgcccgg gtcgccgacc ccgtgcaggt agacgcagac gtcgccgcgc
    11581 ggctccgacc agcgctgccg gtagacgccg atcacccgcg cgcccgagcc gagcgagctg
    11641 acccgctcga gcagctccgg ctcgacatcc tccccggccc gcagcaggat ctccggcgtc
    11701 cagcccgccg cctcggcggc cgcgatcaag tcctcgccct cggcggcgaa caaaccggtc
    11761 gcatcccgct ccttcttgcg ctggagcttg tggatctgct tgagctggct gttgtctctg
    11821 gatgtgatca cggaaaccgg tcctggaacg agaaagggcg ccactctggg cgccccggga
    11881 agctcgttcg accgctgacc tggagtcagg cagcggccgc ctcccgggcg gt
//
LOCUS       DATABV010000009        17891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2574242, whole genome shotgun sequence.
ACCESSION   DATABV010000009 DATABV010000000
VERSION     DATABV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17891
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574242"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VHF88_00195"
     CDS             complement(<1..218)
                     /locus_tag="VHF88_00195"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX2160226.1"
                     /translation="MAVVDQVQEDARSALKAGDRERAGALRLIASELQKAVKDGGDDE
                     VAVLQRERKRRLESAAAYRDGGRDDLATA"
     gene            246..2054
                     /locus_tag="VHF88_00200"
     CDS             246..2054
                     /locus_tag="VHF88_00200"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX2160227.1"
                     /translation="MHDGTLILIAGTLLALGIGATLLAGRLRVPGLVLFLVLGMAIGS
                     DGLGLIDFGATESDVELARTIGIIALALILFEGGLAAGWSEIRPVLRGAMPLAGLGTL
                     LTAVITGVLSHLLFGLTLLEGMLLGSVIASTDSAAIFSVLRGSNVKRKVARTLEAESG
                     FNDPIAVLLVLGFIEWIQAPAYGLADMALLMVEQLSIGALTGFAVGAAAIAAFRSVKF
                     ATTGLYPVASIATAAIAFGAADQLGGSGFLAVYLAGLSLGGARIPARRTIDDFHAGLA
                     WVAQIALFFTLGLLVFPAELGDVVGDGLLLAIVLTLVARPIATFVSTSLAGYSPREMT
                     LLSWAGLRGALPVVFATFPVIEGVPQAELFFNLVFFVVITSALVQGATINPLARRLGL
                     TSRAEAVPPPVMEVGTIRRLGAEVLEFPVDADDAVVGRLVNELGLPRDALVNVIVRDD
                     EALLPRGSTEVAAGDRLHIVVRENARADVEALFVRWRDGPIAPAVRRVAAPQGRSPIF
                     SVKPWRDDSGDPAAPEEIEGIAVTRTLRTRREARGALVVLADGRLAITGDGVVATGSP
                     RQLVRYCRERVGRAQSAQAKAWWQEVAGAVLQSSAP"
     gene            complement(2122..2508)
                     /locus_tag="VHF88_00205"
     CDS             complement(2122..2508)
                     /locus_tag="VHF88_00205"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2160228.1"
                     /translation="MDYSIKNLRQVEDSAVKHGYSEMGEARFAHSALNAENTGLAYHV
                     LRPNKRQAFAHRHDKAEEIYVVLSGSGRIKLDDDVVELETMDAVRLAPSVVRALEAGP
                     DGLELLAFGPRHEADGEIVPAETFWE"
     gene            complement(2548..3357)
                     /locus_tag="VHF88_00210"
     CDS             complement(2548..3357)
                     /locus_tag="VHF88_00210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX2160229.1"
                     /translation="MIRLAVRAPAADAEQVLAALLELAPAGVEQVDGDASVEFAVYGA
                     PGELPELDEGEAEVGGIRVTVSGREVPDDWAERWKRFHAPVLVADRLYVRPPWEEAPL
                     RPGVIDLRIDPGRAFGTGAHATTRLCLELLAGLARERPHGSLCDLGCGSGVLAIAAAK
                     LGYGPVEAVDSDALAVDATRLNARDNGVALEVVRRANLREEPPPAADVVVANLMRPLL
                     LRVAELIDHRPRALIVSGLLDHEADEVAAAFAPLEQLRRLSDRGWTALLLT"
     gene            complement(3354..4478)
                     /gene="dnaJ"
                     /locus_tag="VHF88_00215"
     CDS             complement(3354..4478)
                     /gene="dnaJ"
                     /locus_tag="VHF88_00215"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX2160230.1"
                     /translation="MRRDAYDVLGVERGADERQIKKRFRVLARELHPDVNPEPDAEER
                     FKEAAECYEVLSDSERRQIYDRYGWEGLDSRGKAPHAHGFGSFSDIFDAFFGGDPFGG
                     GRRGGGPGPVQGGDVGVEAEITLEQAARGVAVTVAFDAVETCDHCHGNRAEPGTPIGT
                     CTRCEGSGQLRAISQTAFGQMVRSQVCDRCGGDGKVATTPCSACRGQGRRAAHRSLDV
                     DVPAGIADGQRIRLTGSGHAGDRGGPPGDLYVLVRVAEDERFVRDGADLVSVVDVSAP
                     DAALGVTVSVPTLDGEQEVDVEPGTQPGTVVTLRGQGMPLIGRDRRGDQRVVLNVVIP
                     RNLDERQRELLAELARTITADNLHEPRRESIVDKVRRALG"
     gene            complement(4479..5504)
                     /gene="hrcA"
                     /locus_tag="VHF88_00220"
     CDS             complement(4479..5504)
                     /gene="hrcA"
                     /locus_tag="VHF88_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX2160231.1"
                     /translation="MQVTARQQFLLRKVVEGYADLGHPVGSKWLAEQSDVPWGPSTVR
                     YELARLEELGLLEHPHTSAGRVPTNRGYRRYVDELLEQGGLPAPRKPVELSDMRREVD
                     EAMRATTEQLSQLTDLLALVTAPPIETATIHRVEVLALQPSVAMVVVITSTGGVTKRV
                     IPFEDPLDRGLIDWAGAYLNEALGGLDVGSRLLQGKLADPELSEREREFLAQLAPAFT
                     ELADSAEHTLFYDGAARLISEQRFQELPQVSDLMDVLQRRQALLSMLRASLSESSVYL
                     RIGQENPAPELRSLSMVAANYGVARRNLGAVGVLGPVRMDYPGAIIAVRQAATELSRF
                     VAEVYDE"
     gene            complement(5528..5914)
                     /locus_tag="VHF88_00225"
     CDS             complement(5528..5914)
                     /locus_tag="VHF88_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX2160232.1"
                     /translation="MDVGDSIPELRVTPDRFLPHRYAGASGDFNPIHIDKEFATQVGL
                     PGNILHGLYGMALVARANAAAAGGDPRSLKRLSVQFRGMGVPEQEIVVSGTVTAIDGD
                     STTVETVAEQSGRQIIKNATAELRGV"
     gene            complement(5916..6356)
                     /locus_tag="VHF88_00230"
     CDS             complement(5916..6356)
                     /locus_tag="VHF88_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2160233.1"
                     /translation="MPVDASAQGKQYPPFEYEVGKEKIREYALAVGEDNPVYFDRVAA
                     RAAGLRDVAAPPMFAVVYSAGAMGPAILDPEVGIDFARMVHGGQEFAWGEPVCSGDTI
                     TTAAKCREIFEKDGKGFYVFESVSSNQDGQEVVRGTWTNIVRGV"
     gene            complement(6405..7268)
                     /locus_tag="VHF88_00235"
     CDS             complement(6405..7268)
                     /locus_tag="VHF88_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2160234.1"
                     /translation="MAALETAPNPTTDSLQLFLDGIAKTRLLTAAEELDLAKRIERGD
                     LDAKNRMTEANLRLVVSIAKRYRNQGLDFMELIQEGSIGLVRAVEKFDYRKGFKFSTY
                     ATWWIRQAISRALADKSRTIRIPIHINQQLQKLDGAQRKLEAARDRDPSFEEIAAASG
                     LDIDPDEAELIMRASRAPVSLDKPVGGGEDDAAELGHLIPDEGAVSPFEAAAEALDRS
                     RLALALENLSYRERRIVELRYGLFGEQPRTLDDVGRMFGLTRERARQIEEHAVRKLAS
                     LAEAQGLREAA"
     gene            complement(7336..9150)
                     /gene="lepA"
                     /locus_tag="VHF88_00240"
     CDS             complement(7336..9150)
                     /gene="lepA"
                     /locus_tag="VHF88_00240"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX2160235.1"
                     /translation="MRAPASNIRNFSIIAHIDHGKSTLADRILEVTRTVEARDHRPQL
                     LDSMDLERERGITIKAQAVRVEYTAKDGETYRLHLIDTPGHVDFTYEVSRSLAACDGA
                     LLVVDASQGVEAQTVANTYLAVDAGLELIPVMNKIDLPGAEPERVAGEIAELLGEPAD
                     EVIRMSAKTGEGVTEVLEAIVANVSSPEGDGDAPARALIFDSEFDQYRGVIAYVRVVD
                     GTLRKGDAITAMQTGTQADIDEIGFFGPAMTPVDELQAGEVGYIITGIKDVSLLRVGD
                     TLTPKARPAAEPLPGYREVKPMVFCGLFPVETDRFADLRDALEKLSLNDAALSWEPET
                     SQALGFGFRCGFLGLLHMDIVRERLEREYDLELLATTPNVEYDCTLTNGEEVVVHSPN
                     DMPDPGVIAEVREPYLKATILCPKDAVGAVMELCQERRGTHVGMSFLSEQRVQLHYDL
                     PLAEVVLDFFDQLKSRTKGYASLDYELTGMRPSNLVKLDILLAGDQVDALSMVVHKDK
                     AYDAGRGLAERLRKQIPRQQFEVAIQAAIGSHIIARESVKAVRKDVIAKCYGGDITRK
                     RKLLEKQKQGKKRMKQVGRIEVPQEAFLSVLTLGDDGD"
     gene            complement(9161..9595)
                     /locus_tag="VHF88_00245"
     CDS             complement(9161..9595)
                     /locus_tag="VHF88_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX2160236.1"
                     /translation="MPDERMDVEQQIERLNTALRLQGRSALQYTLTGGSMIGLEHQLL
                     AERFGEFARAEIDDARRLVEKIAALGGEPTAEVAPPRFDPEPRQALQHLVDSETEALT
                     ALQDAIEPTGREAASEAVEHRLEHMIMRKQEQVDVLLRALRT"
     gene            complement(9652..11268)
                     /gene="murJ"
                     /locus_tag="VHF88_00250"
     CDS             complement(9652..11268)
                     /gene="murJ"
                     /locus_tag="VHF88_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX2160237.1"
                     /translation="MADPTTAAPAPREESAGERGGRLARSTAFFSAATGLSRLLGLAR
                     EIVAASLFGVSAAMSAFTIAFQVPNLVRALFADAALQGAFVPVFTELVEKGKRREAFH
                     VASSLFFLILLVLGLTTALFVLLAEPLMSLIAPGFDDQPALRDLTVGLAQLMFPIVLL
                     LALSGLIVGMLNSFEHFSVPALAPVAWNLVIIAALVAVTPLFDGQDRVYAYAIGVLVG
                     TLVQFLLPIPWLRGRGGHFSFALDWRSAHVRRVLWLMLPVTLALGMINLSLLINSLFG
                     TLVSDSAPAAIDKAFRIYQLPQGLFSISIATVLFPTLSKLAARGANDDLRATMSNGVR
                     QICLLLVPSAVLMAVLAEPITRLLYERGEFGPEATDLVTTALVWWSISLPFQGVSLLF
                     SRTFFGLQQPWATTALAVVNMAVNAAIAFALYEPLGISGIVLGTVAGTLVMTVAQGWI
                     LRRELGGIEGRELVLAVLRMLAAAAALAAAAYLAWLGLDELLGRSLPAQVLSVGVGVG
                     AGVAAYAVAVWALRLPEARQVIRLIRGRRA"
     gene            11307..11594
                     /gene="rpsT"
                     /locus_tag="VHF88_00255"
     CDS             11307..11594
                     /gene="rpsT"
                     /locus_tag="VHF88_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX2160238.1"
                     /translation="MTDHLIMANIASQKKRILRTERERDENRRYTSAIKTYFRRLEAA
                     VESGDAETVEAEFRALVSRIDRAVRSGALHKNTGARKKARAARLRASTTDA"
     gene            11618..13099
                     /locus_tag="VHF88_00260"
     CDS             11618..13099
                     /locus_tag="VHF88_00260"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2160239.1"
                     /translation="METRLLIAGEQVEGAGDRLAVENPATEQIVAEVGTASPEQVDTA
                     IAGARDAFADWSTLPAAERAELLHEVANRLRARSDELARAMTLEGGKPLIENSDEVGW
                     TAAAFDYYAEMGRNFAGRVIPPIESSQLALVLKEPLGVVGAIVPWNYPLLLLAWKLAP
                     ALAAGNAVVCKPSELTPISTLMLAPCLDHLPAGTVSLLAGAGDVGERIVADERVDCVA
                     FTGSVEVGKRIAARCSERIARINLEMGGKDPFIVCADVAADVEVAARGGAWAAYLNAG
                     QVCTSAERFYVEESLFDDYVAAFAEHTRSLVVGDPLDPRTDIGPMVSARQRAKTATQV
                     DAALAAGAELVAGGDRAGHERGHFYAPAVVVGAPSDTDLLREETFGPVAPIVPVRSLD
                     EAIELANGTRFGLGANVYTRDLATAVRCMRELKAGTVWINDPLTDNDAGPFGGFKQSG
                     LGRELGQEGLEAFQETKHVHMETRIEAKDWWYPYGGGGPPHGP"
     gene            complement(13100..14053)
                     /gene="holA"
                     /locus_tag="VHF88_00265"
     CDS             complement(13100..14053)
                     /gene="holA"
                     /locus_tag="VHF88_00265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX2160240.1"
                     /translation="MADLKPVYLVCGDDDAKIDAWRARVRKRAETENGPGALEAFDAR
                     TDEAEAVVASLAALTFATGTRYLMVDDVGAWKAADVAPLIEAIATMPPETVLVLVVRG
                     KPLKSLVTAVEQAGGELRQHAAPKPWELPKWATGRARELGLRLEGEAAKALVAIAGPS
                     QQRLSRELEKLAIALYPEATASVEDVERLAAGEASPKVYDLADAVVSGNVEAAIELAE
                     ELSEWGERPSRFVYPVVGRLREVHRAVELLEGGMTEKELAGALKMPPWRAKKVVVLAR
                     RADREALERATCRFADLEVELRGGGSLDEATAVTLALARAA"
     gene            complement(14063..16252)
                     /locus_tag="VHF88_00270"
     CDS             complement(14063..16252)
                     /locus_tag="VHF88_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX2160241.1"
                     /translation="MRPTLVAVLVVGAAVVGQARLEAIDGAAQRLKPGDLVGGRAHLL
                     ERPSRGPFGASAEVELVDGPARATPLLARLPRDVRLPEGAGPGTALRLRGTFRRATSD
                     GEFDFAAHLRRRGIAGELLVERMRFTGGRRGGVWGTLDLIRERALRGVAAELPPRLGA
                     LGQGMVLGADEEVSAAVRDDFRDAGLAHVLAASGTNVMLLVALAIPLLAAAGLSHSAR
                     IPVLIALVALYVPLAGGGPSIQRAGVMGIAGLLAMLLTRPASRWYALLLAACVTLAWN
                     PRAWQEPGWQLSFAAVAGILVLAPGIGRRLFALPRLLADGIALTVAATVATAPLIAHH
                     FDSVSLAGLPANVVALPLVAPIMWLGMLRALLGQLPAAEPVGGLLGLLLEPLLAGFER
                     LAALFADAPGSTIALGLAGPLEIAGAYAALTALALLTRRSLRRLDTRASAAVAYLRRL
                     PRRRRLALVAVAAATVALLLARGLSPQGPPATLTVTFLDVGQGDATLVQHPDGSAVLF
                     DGGPPEGGVARLLRRAGVRRLSAVVMTHASRDHHGGLAEVVERFSIDLLLDGGDGTRD
                     VGFRAVVAAARARGARRVEAVAPLTLRAGGLSVELLSPGPRPPGPPPEDPNPRAVVAI
                     VRSDGFDLLLSGDAESETLLPLGLPDVDAMKVAHHGSADPGLPELLAQTRPEIAAIPV
                     GENSYGHPAPSTLAALRAAGVRAWRTDRDGNVRVSVAHGDVTVATGP"
     gene            complement(16249..16833)
                     /locus_tag="VHF88_00275"
     CDS             complement(16249..16833)
                     /locus_tag="VHF88_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2160242.1"
                     /translation="MDDRGWPAVAWGVVAVVAVLLGVRLVGGGDPAPPPVELGVAGAG
                     GQRSGDRAERPLGPFVHVAGAVRRPGLYRLSAGARVAAAVRRAGGPASDADLDLINLA
                     ARLQDGQQVVVPAEATPGVAVGGGDAAASPPISLGSATVDQLDELDGIGPTLAERIVE
                     HRQAKGGFGSLDQLAEVEGIGEKRLQALKAALAP"
     gene            complement(16933..>17891)
                     /locus_tag="VHF88_00280"
     CDS             complement(16933..>17891)
                     /locus_tag="VHF88_00280"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2160243.1"
                     /translation="SWYFLRYCDARNDSAPWDRDVLAGWMPVDQYIGGVEHAILHLMY
                     ARFFTKAFADIGLLDVQEPFARLFTQGMVTRDGAKMSKSKGNVVSPRELVDEFGADAA
                     RCYILYVGHPAEGGDWNDKGIEGIARFLARLWRAAADAAERTDPVEGPAGEPTAVLRK
                     AHWAIDKVTTDLGERFATHTAIAAVIELVNDITKQRDDLLATPDGRSQYRFAAATAGS
                     LIFPFAPHLGSEVYELLTGRRVWEEPWPQADPALLAADSVTIVVQLNGKVVDRFEAPA
                     ALAEDEQERLARASQRVAERLDGREIVKAIVVPGRLVNLVAR"
BASE COUNT         2533 a         6424 c         6412 g         2522 t
ORIGIN      
        1 gcggtcgcga ggtcgtcgcg cccgccgtcg cggtaggcgg ccgccgactc gagccgccgc
       61 ttgcgctcgc gctgcagcac ggcgacctcg tcgtcgccac cgtccttgac cgccttctgc
      121 aactcggagg cgatcagccg cagggcgccg gcgcgctcgc ggtcgcccgc cttgagcgcg
      181 gaacgcgcgt cctcctggac ctgatcgaca accgccatcg aaccttaagt ctaggctgcc
      241 cgccaatgca cgacggcacg ttgatcctga tcgccggcac gctgctggcg ctcgggatcg
      301 gagcgacgct gctcgccggt cgcctgcggg tgcccggcct ggtgctgttc ctggtgctgg
      361 gcatggcgat cggctcggac ggcctcgggc tgatcgactt cggcgcaacc gagagcgacg
      421 tcgagctcgc gaggacgatc gggatcatcg cgctcgcgct gatcctcttc gagggtggtc
      481 tggcggcggg ctggagcgag atccgtccgg tgctgcgcgg cgcgatgccg ctcgccggac
      541 tcggaacgct gttgaccgcc gtgatcacgg gcgtgctgtc gcacctgctg ttcggcctca
      601 cgctgctcga ggggatgctg ctcgggtcgg tcatcgcatc gaccgacagc gcggcgatct
      661 tctcggtgct gcgcggctcg aacgtcaagc ggaaggtggc gcgcacgctc gaggcggagt
      721 cgggcttcaa cgacccgatc gcggtcctgc tcgtgctggg cttcatcgag tggatccagg
      781 ccccggccta tgggctcgct gatatggcgc tgctgatggt cgagcagctc tcgatcgggg
      841 cgttgaccgg ctttgcggtt ggggccgcgg cgatcgcggc cttccgtagc gtgaagttcg
      901 cgacgaccgg cctctacccg gtcgcgtcga tcgcgacggc ggcgatcgcc ttcggcgccg
      961 ccgaccagct tggcggctca ggcttcctcg cggtctacct cgcgggcctc agcctcggtg
     1021 gcgcgcgcat cccggcgcgc cggacgatcg acgacttcca cgccggcctg gcgtgggtgg
     1081 cccagatcgc gctcttcttc acgctcggct tgctggtctt cccagccgag ctcggcgatg
     1141 tcgttggcga cgggctgctg ctcgcgatcg ttctaacgct ggtcgcccgc ccgatcgcca
     1201 cgttcgtatc gacgagcctg gccggctact ccccgcgcga gatgacgctc ctcagctggg
     1261 ccggcctgcg cggtgcgctg ccggtcgtgt tcgcgacctt cccggtgatc gagggcgttc
     1321 cgcaggccga gctgttcttc aacctggtct tcttcgtggt gatcacgtca gccctcgtcc
     1381 agggcgcgac gatcaacccg ctcgcgcgtc ggctcggcct taccagccgg gcggaggccg
     1441 tgccgccgcc ggtgatggag gtcggcacga tccgccgcct gggcgcggag gtgctggagt
     1501 ttccagtcga cgccgacgat gcggtcgtcg gccggctggt gaacgagctc ggcctgccgc
     1561 gcgacgcgct ggtcaacgtg atcgtccgcg acgacgaggc gctgctgccg cgcggctcga
     1621 ccgaggtcgc cgccggagac cgcctgcaca tcgtcgtccg cgagaacgcg cgcgccgacg
     1681 tcgaggcgct cttcgtccgt tggcgcgacg gtccgatcgc gccggccgta cggcgcgtcg
     1741 ccgctccgca gggccgcagc ccgatcttca gcgtcaagcc gtggcgcgac gactcgggcg
     1801 atccggccgc accggaggag atcgagggca tcgcggtcac gcgcacgctg cgcactcggc
     1861 gcgaggcccg cggcgcgctc gtcgtgctcg ccgacggccg gctcgcgatc accggcgacg
     1921 gagtggtcgc gaccggcagc ccacgccagc tcgtccgcta ctgccgcgag cgcgtcggcc
     1981 gcgcccagag cgcacaggcg aaggcgtggt ggcaggaggt cgccggcgcc gtcctccaga
     2041 gctcagctcc ctgagtcttg acgagcccac cgaccaacca gccacgatgc cgcgtgggtc
     2101 cgggggcgtg agccctcggt cctactccca gaaggtctcg gcgggaacga tctcgccgtc
     2161 ggcctcgtgg cgcgggccga acgcgagcag ctcgagcccg tcgggaccgg cctcgagggc
     2221 gcgcacgacg ctcggggcga gccggacggc atccatcgtc tcgagctcga ccacgtcgtc
     2281 gtcgagcttg atccgcccgc tgcccgagag gaccacgtag atctcctccg ccttgtcgtg
     2341 gcggtgggcg aaggcctgcc gcttgttcgg ccgcagcacg tggtaggcca gcccggtgtt
     2401 ctcggcgttg agggcggagt gcgcgaagcg tgcctcgccc atctcggagt agccgtgctt
     2461 gacggccgag tcctcgacct ggcgcaggtt cttgatcgaa tagtccatgg gcgcacacta
     2521 ccccggcggc ccgcctccgc cgaccggtta ggtgagcagc agagccgtcc aaccgcggtc
     2581 ggagagacga cgcagctgct cgagcggcgc gaaggcggcg gcgacctcgt cggcctcgtg
     2641 gtcgagcagg ccggagacga tcagggcgcg cggacggtgg tcgatcagct ccgcgacgcg
     2701 cagcagcagc ggccgcatca agttggctac gacgacatca gcggcgggcg gcggctcctc
     2761 gcgcaagttg gcgcgcctaa cgacctcgag cgcaacgccg ttgtcgcgtg cgttgagccg
     2821 cgtcgcgtcg accgccaggg cgtcggagtc gaccgcctca accgggccgt agccgagctt
     2881 cgcggcggcg atcgcgagca cgccggagcc gcagccgagg tcgcagagcg agccgtgcgg
     2941 tcgttcgcgg gcgaggccgg caagcagctc gaggcagagg cgggtcgtcg catgggcgcc
     3001 cgtcccgaac gcacggcccg gatcgatccg aaggtcgatc acgccgggcc gcagcggcgc
     3061 ctcctcccac ggcggacgga cgtagaggcg gtccgcgacg agcacgggcg cgtggaagcg
     3121 cttccagcgc tccgcccagt catccggcac ctccctgccg gagaccgtga cgcggatgcc
     3181 gccgacctcg gcctcgccct cgtcgagctc cggcagctcg cccggcgcgc cgtagacggc
     3241 gaactcgacg ctggcgtcgc cgtcgacttg ctccacgccc gcgggcgcca gctcgagcag
     3301 cgcggcgagg acctgctcgg cgtcggcggc cggcgcgcgg accgcgagcc ggatcacccc
     3361 agcgcccggc ggaccttgtc gacgatcgac tctcgccgcg gctcgtgcag gttgtcggcg
     3421 gtgatcgtcc gcgccagctc ggcgagcagc tcgcgctgac gctcgtcgag gttgcgcggg
     3481 atcacgacgt tgagcacgac gcgctggtcg ccgcggcggt cgcggccgat cagcggcatg
     3541 ccctgaccgc gcagcgtgac caccgtcccg ggctgggtcc cgggctcgac gtcgacctcc
     3601 tgctcgccgt cgagcgtcgg cacgctgacg gtcaccccga gcgccgcgtc gggagccgac
     3661 acgtcgacga cgctgaccag gtctgcgccg tcgcggacga agcgctcgtc ctcggcgacc
     3721 cggacgagca cgtagaggtc tccgggcggg ccgccgcggt cgccggcgtg cccgctgccg
     3781 gtcagccgga tccgctggcc gtcggcgatc ccggctggga cgtcgacgtc gagcgagcgg
     3841 tgcgccgcgc gccggccctg gccccggcat gcgctgcacg gcgtcgtggc gaccttgccg
     3901 tcgccgccgc agcggtcgca gacctggctg cgaaccatct gaccgaacgc ggtctgggag
     3961 atcgcacgca gctggccgct gccctcgcag cgcgtgcagg tcccgatcgg cgtgcccggc
     4021 tcggcgcggt ttccgtggca gtggtcgcag gtctcgacgg cgtcgaacgc gacggtgacg
     4081 gccacgccgc gggctgcctg ctcgagcgtg atctcggcct caacgcccac atcaccgccc
     4141 tggacgggac ccggtccccc gccgcggcga ccgccgccga acggatcgcc gccgaagaac
     4201 gcgtcgaaga tgtcggagaa cgagccaaat ccgtgcgcgt gcggcgcctt gccgcgcgag
     4261 tcgaggccct cccagccgta gcggtcgtag atctggcgcc gctcgctgtc gctgagcacc
     4321 tcgtagcact cggcggcctc cttgaaccgc tcctcggcgt cgggctcggg attgacgtcg
     4381 gggtgcagct cgcgcgcgag cacgcggaag cgcttcttga tctggcgctc gtcggcgccg
     4441 cgctcgaccc cgagcacgtc gtaggcgtcg cgtctcatct actcgtcgta gacctcggcg
     4501 acgaagcgcg acagctccgt cgccgcctgg cggacggcga tgatcgcgcc cgggtagtcc
     4561 atccggaccg gcccgagcac gccaaccgcg ccgaggttgc gccgtgcgac gccgtagttg
     4621 gcggccacca tgctcagcga gcgcagctcc ggcgccgggt tctcctgccc gatgcgcagg
     4681 tagacgctcg actccgacag cgacgcccgc agcatggaca ggagcgcctg gcggcgctgc
     4741 aggacgtcca tcaggtcgga cacctgaggc agctcctgga agcgctgctc ggagatcagt
     4801 cgcgccgcgc cgtcgtagaa gagtgtgtgc tcggcgctgt cggccaactc ggtgaacgcg
     4861 ggcgccagct gggcgaggaa ctcgcgctcg cgctcgctga gctcggggtc ggcgagcttt
     4921 ccctggagca ggcgcgagcc gacgtcgagc ccacccagcg cctcgttgag gtacgcgccg
     4981 gcccagtcga tcaggccgcg gtcgagcgga tcctcgaacg ggatcacgcg cttggtgacg
     5041 ccgccggtcg aggtgatcac gacgaccatc gcgacgctcg gttgcaacgc gagcacctcg
     5101 acacggtgaa tcgtcgcggt ctcgatcggc ggcgcggtga ccagcgccag caggtcggtc
     5161 agctgcgaga gctgctcggt ggtcgcgcgc atcgcctcgt cgacctcgcg ccgcatatcg
     5221 gacagctcga cgggcttgcg cggcgccggc aggcctccct gctcgagcag ctcgtcgacg
     5281 tagcggcggt agccgcggtt ggtcggcact cgaccggctg acgtgtgcgg gtgctcgagc
     5341 aggccgagct cctccagccg ggcgagctcg tagcgcaccg tcgagggtcc ccaggggacg
     5401 tcggactgct cggccagcca cttcgagcca accggatggc caaggtccgc gtaaccctcg
     5461 acgaccttgc ggaggaggaa ctgctgccga gccgttacct gcacgccttg gattgtgacg
     5521 gacgctctca gacgccccgc agctctgccg tggcgttctt gattatctgc cgacccgact
     5581 gctcggccac ggtctcgacc gtagtgctgt ccccgtcgat cgccgtgacg gtgccgctga
     5641 ccacgatctc ctgctccggc acgcccatgc cgcggaactg gaccgacagc cgcttcagcg
     5701 agcgcgggtc gccgcccgcc gccgcggcgt tcgcccgcgc gaccagggcc atcccgtaga
     5761 ggccgtggag gatgttgccc ggcaggccga cctgcgtcgc gaactccttg tcgatgtgga
     5821 tcgggttgaa gtcgcccgag gcgccggcgt aacgatgcgg caggaagcga tccggtgtga
     5881 cgcgcagctc gggaatcgag tcgcccacgt ccatctcaga cacccctcac gatgttcgtc
     5941 caggtcccgc gcacgacctc ttgtccgtcc tggttgctcg agaccgactc gaacacgtag
     6001 aagcccttgc cgtccttctc gaagatctcc cggcacttgg cggcggtggt gatcgtgtcg
     6061 cccgagcaga cgggctcgcc ccaggcgaac tcctggccgc cgtggaccat gcgcgcgaag
     6121 tcgatgccga cctcgggatc gaggatcgcc gggcccatcg caccggccga gtagacgacc
     6181 gcgaacatcg gcggagccgc gacgtcgcgc aggccggcgg cgcgggcggc gacacggtcg
     6241 aagtagacgg ggttgtcctc gccgaccgcg agggcgtact cgcggatctt ctccttgccg
     6301 acctcgtact cgaacggcgg atactgcttg ccctgggcgc tggcgtctac cggcacgcga
     6361 cctcccgatc tcgctcgtcg gttgcggcgc ggcagtctag tcgtctaggc ggcttcgcga
     6421 aggccctgtg cctccgcgag cgaggcgagc ttgcgaacgg cgtgctcctc gatctggcgc
     6481 gcccgctcgc gggtcaggcc gaacatccgg ccgacgtcgt cgagcgtccg cggctgctcg
     6541 ccgaagaggc cgtagcgcag ttcgacgatc cgccgctcgc ggtaggagag gttctcgagc
     6601 gccagcgcga ggcgcgagcg gtcgagcgcc tcggcggcgg cctcgaaggg cgagaccgcg
     6661 ccctcgtcgg gaatcaggtg tcccagttcc gcggcgtcgt cctcgcctcc gccgaccggc
     6721 ttgtcgagcg agaccggcgc ccgcgaggca cgcatgatca gctcggcctc gtccgggtcg
     6781 atgtcgagcc cggaggcggc ggcgatctcc tcgaaggagg ggtcgcggtc gcgagcggct
     6841 tccagcttgc gctgcgcgcc gtccagcttc tgaagctgct ggttgatgtg gatcgggatg
     6901 cggatcgtgc gtgacttgtc ggccaacgca cgcgagatcg cctggcggat ccaccatgtc
     6961 gcgtaggtcg agaacttgaa gcccttgcgg tagtcgaact tctcgacggc gcgcacgagg
     7021 ccgatcgacc cctcctggat cagctccatg aagtcgaggc cctggttgcg gtagcgcttg
     7081 gcgatcgaga cgacgaggcg caggttcgcc tcggtcatcc ggttcttcgc gtcgaggtcg
     7141 ccgcgctcga tccgcttggc gaggtcgagc tcctccgcgg cggtcagcag gcgcgtcttg
     7201 gcgatgccgt cgaggaagag ctgcagcgag tcggtcgtcg ggttcggtgc tgtctccaat
     7261 gcggccatgg cggagaagct acgcccgcgc ccttaagcgt tcctaagctg tgcgcaaagg
     7321 ttttcttaag ttgtctcagt cgccgtcgtc accgagtgtg aggaccgaca ggaacgcctc
     7381 ctgcgggacc tcgatgcggc cgacctgctt catccgcttc tttccctgct tctgcttctc
     7441 gagcagcttg cgcttgcgcg tgatgtcgcc gccatagcac ttcgcgatca cgtccttgcg
     7501 gaccgccttg accgactcgc gggcgatgat gtgcgagccg atcgccgcct ggatcgcgac
     7561 ctcgaactgc tgccgtggta tctgcttgcg caggcgctcc gcgagaccgc gtcccgcgtc
     7621 gtaggccttg tccttgtgga cgaccatcga gagcgcgtcg acctggtcgc cggcgagcag
     7681 gatgtcgagc ttgacgaggt tcgacggccg catcccggtc agctcgtagt ccaacgacgc
     7741 gtaacccttg gtgcgcgact tgagctggtc gaagaagtcg agcacgacct cggcaagtgg
     7801 caggtcgtag tgcagctgga cgcgctgctc ggacaggaag ctcatcccaa cgtgcgtgcc
     7861 gcgccgctcc tggcagagct ccatgacggc gccgacggcg tccttcgggc agaggatcgt
     7921 cgccttgagg taaggctcgc gaacctcggc gatcacgcct gggtccggca tgtcgttcgg
     7981 cgagtggacg acgacctcct ctccgttggt cagcgtgcag tcgtactcga cgttcggcgt
     8041 cgtcgcgagc agctcgaggt cgtactcgcg ctcgagccgc tcacgcacga tgtccatgtg
     8101 cagcaggccc aggaacccgc agcggaagcc gaagccgagg gcctggctgg tctcgggctc
     8161 ccaggaaagc gccgcgtcgt tgagcgacag cttctcgagc gcgtcgcgga ggtcggcgaa
     8221 gcggtcggtc tcgaccggga agaggccgca gaagaccatc ggcttgacct cgcggtagcc
     8281 gggcagcggc tcggcggccg gtcgcgcctt cggcgtcagc gtgtcgccga cgcgcagcag
     8341 cgacacgtcc ttgatgccgg tgatgatgta gccgacctcg ccggcctgca gctcgtcgac
     8401 cggcgtcatc gccggcccga agaacccgat ctcgtcgatg tcggcctggg tgccggtctg
     8461 cattgcggtg atcgcgtcgc ccttgcgcag ggtgccgtct acgacccgga cgtaggcgat
     8521 cacgccgcgg tactggtcga actccgagtc gaagatcagc gcccgcgccg gagcgtcgcc
     8581 gtcgccctcc ggcgacgaga cgttcgcgac gatcgcctcc agcacctccg tcacgccctc
     8641 cccggtcttc gccgacatgc ggatcacctc gtcggccggc tcgccgagca gctcggcgat
     8701 ctcgccggcg acccgctcgg gctcggcgcc cggcaggtcg atcttgttca tcaccgggat
     8761 cagctcgagg ccggcgtcga ccgccaggta ggtgttggcg acggtctggg cctcgacgcc
     8821 ctgcgaggcg tcgaccacca gcagcgcccc gtcacaggcg gcgagcgagc gcgacacctc
     8881 gtaggtgaag tcgacgtggc ccggcgtgtc gatcaggtgg aggcgatagg tctcgccgtc
     8941 cttggcggtg tactccacgc gaaccgcctg cgccttgatc gtgatcccgc gctcgcgctc
     9001 gagatccatc gagtcgagca gctgcgggcg atggtcgcgc gcctcgaccg tccgcgtgac
     9061 ctcgaggatg cgatcggcca gcgtcgactt gccgtggtcg atgtgggcga tgatcgagaa
     9121 gttgcggatg ttcgacgcgg gtgccctcac ccgcttgatc ctaggtgcgg agggcgcgga
     9181 gcaggacgtc gacctgctcc tgcttgcgca tgatcatgtg ctcgaggcgg tgctcgacgg
     9241 cctctgaggc ggcctcgcgg ccggtcggct cgatcgcgtc ctggagcgcg gtgagcgcct
     9301 cggtctcgga gtcgacgagg tgctgcagcg cctgcctcgg ctccgggtcg aagcgcggcg
     9361 gggcgacctc ggccgtcggc tcgccgccga gcgcagcgat cttctcgacc agccggcggg
     9421 cgtcgtcgat ctccgctcgc gcgaactcgc cgaagcgctc ggcgagcagc tggtgctcca
     9481 gcccgatcat gctgccgccg gtcagcgtgt actgaagcgc ggagcggccc tgcagccgca
     9541 atgccgtgtt gaggcgctcg atctgctgct cgacgtccat ccgctcgtcc ggcatgcagg
     9601 ccgcgtaccc aaggtcgtgt cgctttagtc gcaggccaat gtcgggtcgt cctaggcgcg
     9661 ccgcccgcgg atcagccgta tgacctgccg ggcctccggc agccgcagcg cccataccgc
     9721 gaccgcgtag gcggcgactc ccgccccgac gccgacgccg accgacaaga cctgggccgg
     9781 cagcgaccgg ccgagcagct cgtcgagccc gagccacgcg agataggcgg ccgccgcgag
     9841 tgcggcggcc gcggcgagca tgcgcagcac cgctaggacc agctcgcgac cctcgatgcc
     9901 gccgagctcg cggcgcagga tccaaccctg ggcgacggtc atcaccagcg tcccggcgac
     9961 cgtgccgagc acgatgccgg atatcccaag cggctcgtag agcgcgaatg cgatggccgc
    10021 gttgacggcc atgttgacga ccgcgagcgc cgtggtcgcc cacggctgct gcaggccgaa
    10081 gaacgtgcgc gagaagagca ggctgacgcc ctggaacggc agcgagatcg accaccagac
    10141 gagcgcggtc gtcacgaggt ccgtcgcctc cggcccgaac tcgccgcgct cgtagagcag
    10201 ccgcgtgatc ggctcggcga gcaccgccat cagcaccgcg ctcgggacga gcagcaggca
    10261 gatctggcgc acgccgttgg acatcgtcgc gcgcaggtcg tcgttggccc cgcgcgccgc
    10321 aagcttcgac agcgtcggga agaggaccgt cgcgatcgat atcgagaaca gcccctgcgg
    10381 cagctggtag atccggaagg ccttgtcgat cgcggccggt gcggagtcgg agaccagcgt
    10441 cccgaacagc gagttgatca gcaggctgag gttgatcatc ccgagcgcca gcgtgaccgg
    10501 gagcatcagc cagagcacgc gccggacatg cgcgctgcgc cagtcgagcg cgaacgagaa
    10561 gtggccgccg cggccgcgca gccaggggat cggcagcagg aactggacaa gcgtcccgac
    10621 cagcacgccg atcgcatagg cgtagacgcg atcctggccg tcgaacagtg gcgtcacggc
    10681 gacgagcgcg gcgatgatca cgaggttcca tgcgaccggc gccaacgccg gcacgctgaa
    10741 gtgctcgaag ctgttcagca tcccgacgat cagccccgac agggcgagca gcaggacgat
    10801 cgggaacatc agctgggcca ggccgacggt caggtcgcgc agcgccggct ggtcgtcgaa
    10861 ccccggcgcg atcagcgaca tgagcggctc ggcgagcagc acgaacagcg ccgtcgtcag
    10921 gccgaggacg agcaggatca ggaagaacag gctcgaggcg acgtggaagg cctcgcgccg
    10981 cttgcccttc tcgacgagct cggtgaagac gggcacgaag gctccctgca gggccgcgtc
    11041 ggcgaacagc gcgcggacga ggttcggcac ctggaaggcg atcgtgaacg ccgacatcgc
    11101 cgcgctgacg ccgaacaggc tcgcggcgac gatctcacgc gcaaggccga gcagtcgcga
    11161 gaggccggtg gcggcagaga agaacgcggt gctgcgggcc aagcggccgc cgcgctcgcc
    11221 ggcgctctcc tcccgcggtg cgggcgccgc ggtcgtcgga tcggccatcc ccgctacact
    11281 acgccgccgc cgcgccgttc cctggcgtga cggaccacct gatcatggcc aacatcgcgt
    11341 cacagaagaa gcgcatcctg cgcaccgagc gcgagcgcga cgagaaccgt cgctacacgt
    11401 cggcgatcaa gacctacttc cgccgtctcg aggccgctgt cgagtccggc gacgccgaga
    11461 cggtcgaggc cgagttccgt gcgctggtgt cgcgcatcga ccgcgccgtg cgcagcggag
    11521 cgctgcacaa gaacaccggc gcgcgcaaga aggcccgcgc cgcgcgcctg cgcgcctcga
    11581 caaccgacgc atagcggcgc gcgcctatag tcgggtcgtg gagactcggc ttctgatcgc
    11641 gggcgagcag gtcgagggag ccggcgaccg gctggcggtc gagaaccccg ccaccgagca
    11701 gatcgtcgcc gaggtcggca cggcctcccc cgagcaggtc gacacggcga tcgcgggcgc
    11761 acgcgacgca ttcgccgact ggtccacgct cccggccgcc gagcgagccg agctgttgca
    11821 cgaggtcgcg aatcggctgc gggcacgaag cgacgagctc gcccgcgcga tgacgctcga
    11881 gggcggcaag ccgctgatcg agaactcgga cgaggtcggc tggacggcgg ccgcgttcga
    11941 ctactacgcc gagatggggc gcaacttcgc cggtcgcgtg atcccgccga tcgagtcgag
    12001 ccagctcgcg ctggtgctga aggagccgct cggcgtcgtc ggcgcgatcg tgccgtggaa
    12061 ctacccgctg ctgctgctcg cctggaagct ggctccggcc ctggccgccg gcaacgcggt
    12121 cgtctgcaag ccgtcggagc tgacgccgat ctcgaccctg atgctggcgc cctgcctcga
    12181 ccatctaccg gcaggcaccg tgtcgctgct ggcgggggcc ggcgacgtag gcgagcggat
    12241 cgtcgccgac gagcgcgtcg actgcgtcgc cttcaccggc tcggtcgagg tcggcaagcg
    12301 gatcgccgcg cgctgctccg agcggatcgc gcggatcaac ctcgagatgg gcggcaagga
    12361 cccgttcatc gtctgcgccg acgtagccgc cgacgtcgag gtcgccgcgc gcggcggcgc
    12421 ctgggcggcc tacctcaacg ccggccaggt ctgcacctcg gccgagcgct tctacgtcga
    12481 ggagtcgctg ttcgacgact acgtcgcggc ctttgccgag cacacgcgct cgctggtcgt
    12541 cggtgatccg ctcgacccgc gcaccgacat cggcccgatg gtctcggcgc gccagcgggc
    12601 gaagacggcg acgcaggtcg acgccgcgct ggcggccggc gcggagctgg tcgcgggcgg
    12661 cgatcgggct gggcatgagc gtggccactt ctacgcgccg gcggtggtcg tcggcgcccc
    12721 ctcagacacc gacctgctgc gcgaggagac cttcggcccg gtcgcgccga tcgtgccggt
    12781 gcgctcgctc gacgaggcga tcgagctcgc caacggcacc cgcttcggcc tcggcgccaa
    12841 cgtttacacg cgcgacctcg cgaccgccgt gcgctgcatg cgcgagctga aggccggcac
    12901 ggtctggatc aacgacccgc tgaccgacaa cgacgcgggc ccgttcggcg gcttcaagca
    12961 gtcggggctc ggccgcgagc tcggtcagga gggcctggag gcgttccagg agaccaagca
    13021 cgtgcatatg gagacgcgta tcgaggcgaa ggactggtgg tacccatacg gcggcggcgg
    13081 cccgccgcac ggaccctgat caggcggcgc gtgccagcgc gagcgtgact gcggtcgcct
    13141 cgtcgaggct gccaccgccg cgcagctcga cctcgaggtc ggcgaagcgg caggtggcac
    13201 gttcgagcgc ctcacggtcc gcacgacggg cgagcacgac gaccttcttg gcgcgccatg
    13261 gcggcatctt cagcgccccg gccagctcct tctcggtcat gcccccctcg agcagctcga
    13321 cggcgcggtg cacctcgcgc agcctcccga cgaccgggta gacgaagcgg cttggccgct
    13381 cgccccactc gctgagctcc tccgccagct cgatcgcggc ctcgacgttg ccggagacga
    13441 cggcgtcggc gaggtcgtag accttcgggc tcgcctcgcc ggccgccagc cgctcgacgt
    13501 cctcgaccga ggccgtcgcc tccggataga gcgcgatcgc gagcttctcg agctcgcgtg
    13561 acagccgctg ctggctcggc cctgcgatcg cgacgagcgc cttggctgcc tccccctcga
    13621 gccgcaggcc gagctcgcgc gcgcggccgg tcgcccactt gggcagctcc cagggcttcg
    13681 gcgcggcatg ctggcgcagc tcgccgccgg cctgctcgac cgccgtgacg aggctcttca
    13741 gcggcttgcc gcgaacgacc aggacgagca cggtctcggg cggcatggtt gcgatcgcct
    13801 cgatcagcgg cgcgacatcg gcggccttcc aagcgccgac gtcgtcgacc atcaggtagc
    13861 gcgtcccggt cgcgaaggtc agggcggcga gcgaggcgac gacggcctcc gcctcgtccg
    13921 tcctcgcgtc gaacgcctcg agcgcgcccg ggccgttctc ggtctcggcg cgcttgcgga
    13981 cgcgtgcgcg ccaggcgtcg atcttcgcgt cgtcgtcgcc gcagaccagg tagacgggct
    14041 tgaggtcggc caccgcccga tcctacgggc cggtggcgac ggtgacgtcg ccgtgcgcga
    14101 ccgagacgcg cacgttgccg tcgcggtcgg tgcgccaggc cctgaccccg gcggcgcgca
    14161 gcgcggcgag cgtcgacggc gccgggtgcc cgtagctgtt ctcaccgacg gggatcgccg
    14221 cgatctcggg ccgggtctgg gcgagcagct cgggcaggcc ggggtcggcg ctgccgtggt
    14281 gggcgacctt catggcgtcg acgtcgggca gaccgagcgg cagcagcgtc tcgctctcgg
    14341 cgtcgccgga gagcaacaag tcgaagccgt cgctgcgcac gatcgcgacg acggcgcgcg
    14401 ggttgggatc ctcggggggc ggccccggcg ggcgcgggcc gggcgacagc agctcgacgg
    14461 acagcccgcc ggcgcgcagc gtgagcggcg ctacggcctc gacgcggcgt gcaccgcggg
    14521 cgcgtgcggc cgcgacgacg gcgcggaagc cgacgtcgcg cgtgccgtcg ccaccgtcga
    14581 gcagcaggtc gatcgaaaac cgctcgacga cctcggcgag cccgccgtgg tggtcgcgcg
    14641 acgcatgcgt catcacgacc gccgacagcc gccgtacgcc cgcgcggcgc agcagccgcg
    14701 ccacgccgcc ttccggcggg ccgccgtcga ataggacggc gctgccatcg gggtgctgga
    14761 cgagcgtcgc gtcgccctgg ccgacatcga ggaatgtgac ggtcagggtg gccggcgggc
    14821 cctgtggcga cagcccgcgg gcgagcagca gcgcgaccgt cgcggcggcg accgccacca
    14881 gcgcgagacg gcggcggcgc ggcaggcggc gcaggtaggc gacggcggcc gaggcacgag
    14941 tgtcgaggcg gcgcagcgag cggcgggtga gcagcgcgag cgccgtgagc gccgcgtaag
    15001 cgccggcgat ctccaacggc cccgcgagcc cgagcgcgat cgtgctgcca ggcgcgtcgg
    15061 cgaacagcgc tgccaggcgc tcgaagccag ccaggagcgg ttcgagcagc agaccgagca
    15121 gaccgccgac gggctcggcg gccggcagct ggccgagcag cgcgcgcagc atcccgagcc
    15181 acatgatcgg cgcaaccagc ggcagcgcga cgacgttcgc cggcagtccc gccagcgaca
    15241 cgctgtcgaa gtgatgggcg atcagcggcg cggtcgccac agtcgcggcg acggtcaggg
    15301 cgatgccgtc cgcgagcagc cgcgggagtg caaacagccg gcgaccgatg cccggcgcca
    15361 ggaccaggat ccccgctacg gcggcgaacg acagctgcca gcccggctcc tgccaggcac
    15421 gcgggttcca cgccaacgtg acgcaagccg ccagcagcag cgcgtaccag cgcgacgcgg
    15481 gcctggttag cagcatcgcc agcagacccg cgatccccat cacgccggcg cgctggatcg
    15541 agggtccgcc gccggcgagc ggaacgtaga gcgcgaccaa ggcgatcagg acggggatcc
    15601 gggcgctgtg ggagagcccg gcagcagcga gcagcggtat cgccagcgcg acgagcagca
    15661 tcacgttcgt gcccgaggcc gccagcacat gcgccaagcc ggcatcgcgg aagtcgtcgc
    15721 ggaccgcggc ggagacctcc tcgtccgcgc cgaggaccat cccctgaccg agtgccccga
    15781 gcctgggcgg caactccgcg gcgacgcccc tgagcgcccg ctcgcggatc aagtcgagcg
    15841 tgccccacac gccgccacgc cggccgccag tgaaccgcat ccgctcgacg aggagctcgc
    15901 ccgcgatccc acggcgccgc aggtgggctg cgaagtcgaa ctctccgtcg gaggtggcgc
    15961 gtctgaaggt gcctctcaag cgcagcgccg ttccagggcc ggcgccttcg ggcaggcgca
    16021 cgtcgcgcgg cagccgcgcc agcagcgggg tggcgcgtgc cggaccgtcg accagctcga
    16081 cctccgcgga ggcgccgaac gggccccgcg acggacgttc gagcaggtgc gcgcgacctc
    16141 cgacgaggtc tccgggcttc aagcgctgtg ccgcgccgtc gatcgcctcg agccgtgcct
    16201 gcccgacgac ggccgcccct acgaccagga cagcgacgag cgtcggcctc aaggtgcgag
    16261 cgcggccttc agggcctgca gccgcttctc gccgatgccc tcgacctcgg cgagctggtc
    16321 gagcgatccg aacccgccct tcgcctggcg atgctcgact atccgctcgg cgagcgtcgg
    16381 accgatgccg tcgagctcgt cgagctgatc gacggtcgcc gatccgaggc tgatcggcgg
    16441 cgatgcagcc gcatcgccgc cgcccaccgc caccccgggc gtcgcctctg cgggcacaac
    16501 cacctgctgg ccgtcctgga gccgcgcggc gaggttgatc agatcgaggt ccgcgtcgct
    16561 ggccggtcca ccggcgcgac ggacggccgc ggcgacgcgc gcgccggctg acaggcggta
    16621 gagacccggc ctgcgcacgg ctccggcgac gtggacgaag ggacccaacg gccgctcggc
    16681 gcgatcaccc gagcgctgcc cgcccgcgcc ggccactccg agctcgaccg gcggcggtgc
    16741 gggatcgccc ccgcccacca gacggacgcc cagcagcacg gccacgacgg cgaccacccc
    16801 ccacgccacc gccggccagc cccgatcgtc cacggccgcg acgctaggcg cgcggctgac
    16861 cgcgcaggcg acctagcggt tacgaagccg cgtcagcggc gtaacggcgc ccaccgacag
    16921 ccaccgatgg cgctagcggg cgaccaggtt gacgagccgg ccgggtacga cgatcgcctt
    16981 gacgatctcg cgtccgtcga gccgctcggc cacgcgctgc gaggcacgcg caagccgctc
    17041 ctgctcgtcc tccgcgagcg ccgccggagc ctcgaagcgg tccaccacct tgccgttcaa
    17101 ctgcacgacg atcgtcaccg agtcggccgc cagcagcgcg ggatcggcct gcggccacgg
    17161 ctcctcccag acccgccgcc cggtcagcag ctcgtagacc tcgctgccca ggtgcggagc
    17221 gaacgggaag atcagcgagc ccgccgtcgc cgccgcgaag cggtactgcg agcggccgtc
    17281 gggggtggcg agcaggtcgt cgcgctgctt ggtgatgtcg ttgacgagct cgatcacggc
    17341 ggcgatcgcg gtgtgggtgg cgaaccgctc gccgaggtcg gtcgtcacct tgtcgatcgc
    17401 ccagtgggcc ttgcgcagga cggcggtcgg ctcgcccgcc ggcccctcga ccgggtccgt
    17461 gcgttcggcg gcatcggccg cagcgcgcca gagccgcgcc aggaagcggg cgatcccctc
    17521 gatccccttg tcgttccagt cgccgccctc ggcgggatgg ccgacgtaga ggatgtagca
    17581 gcgggcggcg tcggcgccga actcgtcaac caactcgcgc gggctgacga cgttgccctt
    17641 cgacttcgac atcttcgccc cgtcgcgggt gaccatcccc tgcgtgaaga gccgcgcgaa
    17701 cggctcctgg acgtcgagca ggccgatgtc cgcgaacgcc ttcgtgaaga aacgcgcgta
    17761 catcaggtgc aggatcgcgt gctcgacgcc cccgatgtac tggtcgaccg gcatccagcc
    17821 agcgaggacg tcgcggtccc agggcgcgga gtcgttgcgc gcgtcgcagt agcgcaggaa
    17881 gtaccacgac g
//
LOCUS       DATABV010000010         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2493994, whole genome shotgun sequence.
ACCESSION   DATABV010000010 DATABV010000000
VERSION     DATABV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493994"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1472
                     /locus_tag="VHF88_00285"
     CDS             192..1472
                     /locus_tag="VHF88_00285"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2160244.1"
                     /translation="MKSERRPGPGAEPDAQRPPREVVDRLAELLPEGALDEAVRGLKP
                     DELSGPGGLLSQLAGRVVEAALEAELTEHLGHPPGGRPHGPDVRNGKGRKTVQTDVGP
                     VEVRPPRDRDGSFEPRILPTRATRLAGLDEKVLGLYAGGMTVRDIAAHLSELYGTEIG
                     RDTISRITDAVLEDVQAWRTRPLDAVDPIVYFDAMVVKVREDRSVQNRACYLALGVTC
                     DGDREVLGIWWQDSEGAKFWLAVLNDLRRRGVHDVLIACVDGLKGFPEAIEATFPQAW
                     VQTCIVHRIRASLRYVNYRDQRKVAAALRPIYTAPNADAALEELERFDDEWGERYPAT
                     VQAWRAAWEHVIPFLALPDELRKAVYTTNTIERLHRQIRKAIKTRGHFPDEQAATKLI
                     YLAILKADAKWQRNRTRTTPRAALKIHFGDRFPG"
     gene            complement(1500..2216)
                     /locus_tag="VHF88_00290"
     CDS             complement(1500..2216)
                     /locus_tag="VHF88_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2160245.1"
                     /translation="MRRTVPPSAEIEAQIDQLLAVGVGDNPRESLSELAKLGARLIIQ
                     RAVEEEFDAWLGRARYERRSEAPPGVRNGFRPRRVQTAEGELSVEIPQVRAAAEPFVS
                     TLFPRGTKLLRTEPLRAMVIGAFVRGLSMRDVESLCDEAGLGKLSKSTAARLCSELRE
                     RFEAFQRRDLYDVRLATLFLDATFLAVRRDGPKEGVLVAWGFTEDGERVLLAVMLGMR
                     ESHEDWLALARDRDCPSFCV"
BASE COUNT          375 a          877 c          791 g          312 t
ORIGIN      
        1 gtcgctggcg gcgcacccac gcccggtgct ggaagcgagt tctgtccgat gaggtgtgtg
       61 tggttgtggt gatgcgaccg cggtgtcgtg tcgctgtggc ggcctgagac gccggggccc
      121 gtcgggcgaa ggcggctcgg gcggccacgg cgggcggccc gcagaaggga ccagcggtca
      181 ccatgagcac catgaagtcc gagcggcgcc cgggtccggg cgccgagccc gatgcgcagc
      241 ggccgccgcg ggaggtcgtc gaccgcctcg ccgagctgct gcccgagggt gcgctcgatg
      301 aggccgtccg cgggctgaag cccgacgagc tgtcggggcc gggcgggctt ttgtcgcagc
      361 tggccggccg cgtcgtcgag gcggcgttgg aggccgagct gaccgagcac ctcggccacc
      421 cgccgggcgg gcggccgcac ggccccgacg tccgcaacgg caagggccgc aagacggtcc
      481 agaccgacgt cgggccggtc gaggtgcgcc cgccgcgcga ccgcgacggc agctttgagc
      541 cgcggatcct gcccacgcgc gccacgcgcc tggccggcct cgacgagaag gtcctggggc
      601 tctacgccgg cggcatgacc gtgcgcgaca tcgcggcgca cttgtccgag ctctacggca
      661 ccgaaatcgg ccgcgacacc atcagccgga tcaccgacgc ggtgctcgaa gacgtccagg
      721 cgtggcgcac gcgcccgctc gatgcggtcg atccgatcgt ctacttcgac gccatggtcg
      781 tgaaggtccg cgaggaccgc tcggtccaga accgcgcctg ctacctcgcc ctgggcgtga
      841 cctgcgacgg cgaccgcgag gtcctcggca tctggtggca agacagcgag ggcgcgaagt
      901 tctggctggc cgtcctgaac gacctgcgcc gccgcggcgt gcacgacgtt ctgatcgcct
      961 gcgtcgacgg cctcaagggc ttcccggagg cgatcgaggc cacgtttccg caggcgtggg
     1021 tgcagacctg catcgtgcat cgcatccgcg cctcgctgcg ctacgtcaac taccgcgacc
     1081 agcgcaaggt cgccgcggca ctgcgaccga tctacaccgc ccccaacgcc gacgccgcgc
     1141 tcgaggaact cgagcgcttc gacgacgagt ggggcgagcg ctaccccgcg accgtgcagg
     1201 cctggcgggc ggcgtgggaa cacgtcatcc cgttcctcgc gctgcccgac gagctgcgca
     1261 aggccgtgta caccacgaac acgatcgagc gcctgcaccg ccagatccgc aaggcgatca
     1321 agacccgcgg gcacttcccc gacgaacagg ccgccaccaa gctcatctac ctggcgatcc
     1381 tcaaggccga cgccaagtgg caacgcaacc gcacccggac aacaccccgc gccgccctca
     1441 agatccactt cggagaccgc ttccccggct gaacaacaca tcaaccagtc gccacggcct
     1501 cacacacaga aactcggaca gtctcgatcg cgcgcgagcg ccagccagtc ctcgtgggac
     1561 tcgcgcatcc cgagcatcac ggccaagagc acgcgctcgc cgtcttcggt gaagccccag
     1621 gcgactagca cgccctcctt gggcccgtcg cggcggacgg cgaggaacgt cgcgtccagg
     1681 aacaacgtgg ccaggcggac gtcgtagagg tcgcgtcgct ggaacgcctc gaaccgttcg
     1741 cgcagctccg agcacagtct ggcggcggtc gacttcgaga gtttccccag ccccgcctcg
     1801 tcgcatagcg actcgacgtc gcgcatcgac agcccgcgca cgaacgcgcc gatcaccatc
     1861 gccctcagcg gctcggtgcg cagcagcttc gtgccgcgcg gaaacagcgt cgagacgaac
     1921 ggctcggccg ccgcgcggac ctgcgggatc tccaccgaga gctcgccctc ggcggtctgc
     1981 acgcgcctgg gacggaagcc gttgcgcacc cccggcggcg cctccgaccg gcgctcatag
     2041 cgcgcccggc ccagccacgc atcgaactcc tcctccactg cgcgctggat gatcaaccgg
     2101 gcgcccagct tcgcgagctc cgacagcgac tcgcgcgggt tatcgccgac ccccacggcc
     2161 agcaactggt cgatctgcgc ctcaatctcc gccgacggcg gtacggtacg tctcacggcg
     2221 taggcctcct tcattgtctt ggcggacgtg agggaaccta cgccgtcgcc ttgacagctc
     2281 cggcggattt acagcagttg caggacgcgg ccaaacgcgg cgccgcgact cgctcggcga
     2341 gccagtccga tgtga
//
LOCUS       DATABV010000011         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2414102, whole genome shotgun sequence.
ACCESSION   DATABV010000011 DATABV010000000
VERSION     DATABV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414102"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VHF88_00295"
     CDS             complement(<1..293)
                     /locus_tag="VHF88_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX2160246.1"
                     /translation="MIAPVERDLLELALEQARIGAAEGGVPIGAALVVDGQALGAGRN
                     RRVQDGSAIRHGETDALENAGRQPASVYRRATMVTTLSPCDMCTGAILLYGIPR"
     gene            complement(290..1135)
                     /locus_tag="VHF88_00300"
     CDS             complement(290..1135)
                     /locus_tag="VHF88_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160247.1"
                     /translation="MKVRLVVLLLAAVLLASCQVDAVEPAVAAITPSPRAVDEATREA
                     AAATPVPTWRPTPAPTARLTPSPVPTASPTTTPTPSPQKVAFAFDLYEEGDFVPQHTF
                     EWCVGASIQMAHNLVFADRRSSYEDQQALWELARSHSTNSFNGANPRGWATALTAIGV
                     GDYRLVSIPDYGEALRTAAEAIRDTGRPVGLVMWRGRHAWVMSGFESLGDPALHADFA
                     VTGIRVLDPLYPHGSGIWGPSPEPNRLLTPDELAAQFVIREPRRWSSDLPAGYLLVLP
                     TRGRS"
     gene            1227..1298
                     /locus_tag="VHF88_00305"
     tRNA            1227..1298
                     /locus_tag="VHF88_00305"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1259..1261,aa:Lys,seq:ttt)
     gene            complement(1480..2220)
                     /locus_tag="VHF88_00310"
     CDS             complement(1480..2220)
                     /locus_tag="VHF88_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160248.1"
                     /translation="MLTQVEATPQHRSTWWPARRTPTVNKIVGFGLAAVVAVVVLVIG
                     AQLFSSPSNVGSGAESTPTIQPTRDSSATPSTSPRPSPSAGFSRFNSTMHGISIDYPS
                     GWEIRQATEPWTGGALNFDSAASDVIFDPTLGDRLYIVLASQPGRADPFDHQSDQFDL
                     IDEAGVCDPEGGMGGGNFTVDGASAWGQICAPGIRAATYVEVVAVTTETRRYLIVLVE
                     SGDEPGLRDAYDFEAALETVDLHPDEDR"
     gene            complement(2316..>2792)
                     /locus_tag="VHF88_00315"
     CDS             complement(2316..>2792)
                     /locus_tag="VHF88_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2160249.1"
                     /translation="ADRFLAVSHRILSDIELAEDATQQALLSVWQNLPQLHDPARFEA
                     WSYRLLVRACYAEGRKSRRWAPNLRLLSVDEPMVVDDTSAVVDRDQLERAFQRLSLNH
                     RTVVVLHHYLDLPLDRVADIVGAPLGTVGSRLHYATRQLRSALIADAQPTVREVAQ"
BASE COUNT          458 a          932 c          980 g          422 t
ORIGIN      
        1 cgcggaatgc cgtacagcag gatcgcgccg gtgcacatgt cgcacggcga cagcgtcgtc
       61 accatcgtcg cgcgtcggta gaccgatgcg ggttgccgcc cggcgttctc gagcgcatcg
      121 gtctcaccgt ggcgtatggc gctgccgtcc tggactcgac ggttccgtcc ggcaccgagc
      181 gcctggccgt caacgacgag cgcggccccg atcggtacgc caccctccgc ggcgccgatg
      241 cgcgcctgct cgagggcaag ctccagcagg tcccgctcga cgggcgcgat cacgaccgcc
      301 ctctcgtggg aaggacgagg aggtagccgg ccggcaggtc gctgctccag cggcgcggct
      361 cccggatcac gaactgggcg gcgagctcat cgggtgtcag cagccggtta ggctcgggcg
      421 acggtcccca tatgccgctg ccgtgcggat agagcggatc gaggacgcga atgcccgtca
      481 ccgcgaagtc ggcgtggagc gcgggatcgc cgagtgactc gaacccgctc atcacccagg
      541 cgtgccgtcc acgccacatg acgagcccga cgggacggcc cgtgtcccgg atggcctcgg
      601 cggcggtacg gagcgcctcc ccgtagtcgg gaatcgaaac aagcctgtag tcgcccacgc
      661 cgatcgcggt gagcgccgtt gcccagcccc gcgggttggc gccattgaac gagttcgtcg
      721 agtggctgcg cgccagctcc cacagcgcct gctgatcctc gtacgagctg cgccggtcgg
      781 cgaagacgag gttgtgggcc atctggatgc tggcgccgac gcaccactcg aaggtgtgct
      841 gcgggacgaa gtcgccctcc tcgtagaggt cgaacgcgaa ggcgaccttc tgcggcgacg
      901 gtgtgggcgt tgtcgtcggc gatgccgtcg gaaccgggct gggagtcagc ctcgcggtgg
      961 gcgcgggtgt cgggcgccat gtcggtacgg gcgtcgccgc ggcagcctcc cgcgtcgctt
     1021 cgtcgacagc gcgaggcgac ggtgtgatgg ccgcgacggc cggctcgacc gcgtccacct
     1081 ggcaggaggc gagcagaact gcggcgagga gaagcacgac caggcggacc ttcatcgggc
     1141 gtcatgatca cgcgctacgc cgcagcgagc aatcggccgc cagggcatcg ctggcccgat
     1201 cggcgacaat ggcggcatcg gtgcgggggc cgttagctca gcggcagagc aggggacttt
     1261 taatcctcgt gtcgtgggtt cgaatcccac acggccctct ctgacaacac accgcggaag
     1321 gaacgccgcc tcgtcgccgc aggcgcgtac gtcgcattgg acctcttcag gtggtcgcgt
     1381 ggagacgcaa ggtctgacgc aaagtgaggt cgccgtcagc ccacacggcc ttcaagctct
     1441 tgcatcgacg ccagcgagtg ttcgcgacca cgatgcggtt caacgatcct cgtccgggtg
     1501 gaggtccacg gtctcgagcg ccgcttcgaa gtcgtaggca tcgcgaagcc ccggctcgtc
     1561 gcccgactcg accagcacga tcagatagcg gcgagtctcg gtcgtgacgg ccacgacctc
     1621 cacgtaggtg gcggcacgga tcccgggggc acagatctga ccccacgcac tcgcaccgtc
     1681 cacggtgaag tttccgccgc ccataccgcc ctcggggtcg cacacgccag cctcgtcaat
     1741 caggtcgaac tgatcggact ggtgatcaaa cgggtcggcc cgaccgggct gggaagccag
     1801 gacgatgtac aggcggtcgc cgagcgtggg atcgaagatg acatccgacg ccgccgagtc
     1861 gaagttgaga gctccgccgg tccagggctc ggtcgcctgc ctgatctccc agccggacgg
     1921 gtagtcgatg gagataccgt gcatcgtcga attgaaccgc gagaaccccg ccgagggcga
     1981 cggccgcggc gacgtcgaag gtgtcgcgga cgagtcccgg gtcggctgga tcgtgggcgt
     2041 tgactcggcg cccgagccaa cgttgctggg cgaactgaac agctgggcgc cgatgaccag
     2101 gacgacgacc gctaccaccg cggcgaggcc gaagccgaca atcttgttca cggttggtgt
     2161 cctccgcgcc ggccaccagg tggagcggtg ctgtggagtc gcctccacct gggtcagcac
     2221 cgcccccgcg atgcgggaaa cgtcctcgtg ccggtcctcg tggagccacg accgaatgag
     2281 gcggtcgacg tcgcggtccg tggttctcga gtcggtcatt gagccacctc ccggaccgtc
     2341 ggctgtgcgt cggcaatgag cgccgagcgc agctgccgcg tcgcgtaatg caatctcgat
     2401 ccgaccgtcc cgagcggcgc gccgacgatg tcagctactc gatcgagcgg caggtccagg
     2461 tagtggtgca ggaccaccac cgtccgatgg ttcagggaaa gacgttggaa ggcgcgctcg
     2521 agctggtcgc gatcgacgac cgcgctcgtg tcatcgacca ccatcggttc gtcaaccgat
     2581 agcagcctca ggttcggcgc ccaacggcgg gacttgcggc cctcggcgta gcaggcgcgg
     2641 acgaggagcc gatacgacca ggcctcgaaa cgggccgggt catgcagctg aggaaggttc
     2701 tgccagacgc tcaggagtgc ctgctgcgtg gcgtcctcgg ccagctcgat gtcgctcagg
     2761 atccggtgcg acacggccag gaaccgatcc gc
//
LOCUS       DATABV010000012        27943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2655606, whole genome shotgun sequence.
ACCESSION   DATABV010000012 DATABV010000000
VERSION     DATABV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 27943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27943
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655606"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="mptA"
                     /locus_tag="VHF88_00320"
     CDS             <1..796
                     /gene="mptA"
                     /locus_tag="VHF88_00320"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HEX2160250.1"
                     /translation="VVNEAIDAVVLGEAFKAETLAQHIAERVRDRQEALRAEVKIAAR
                     YPERQNTPASGSATQEIYTVFGVAVASERGTRRLIGVEAHGMTACPCAQEMVADRSRE
                     RLLADGFTDDEIERVLAAVPVATHNQRGIGRLYVGCVEGCTNTVEARTLLRIVEGSMS
                     SEIYELMKRSDEVEVVERAHMHPRFVEDCVREMVRTAVDEFAGFDGDTFLLARQENLE
                     TIHRHSVVAERHGLLGRLRQELTDEVHLQRHVTMREWLEGPAAFAD"
     gene            873..1343
                     /locus_tag="VHF88_00325"
     CDS             873..1343
                     /locus_tag="VHF88_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160251.1"
                     /translation="MTIAHRIPLSEDTALVTALAGTAMAFSHCAEDEAERWLRALRLH
                     GQVGCALQALGVGEAPLESRAAEGPDGAGKVCDEPSASPPLGDAALDRTVREAERRAI
                     ERNAEAVGTADLLLALLDVYGEPMDRALEARGATRDEVIERLAAMGDHPELASG"
     gene            complement(1354..1932)
                     /gene="pgsA"
                     /locus_tag="VHF88_00330"
     CDS             complement(1354..1932)
                     /gene="pgsA"
                     /locus_tag="VHF88_00330"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX2160252.1"
                     /translation="MFPLNLPNVLTLLRILLVPVLVVALGTEAQGGSELAAAVFVLAS
                     LTDGLDGYIARSRQSITTFGKVMDPVADKLLIAAALLTLVSLDRVDAWVAMIVIAREF
                     AVSGLRIAAGQQGVVIPASALGKVKTIVQSAAVLALIAVADPDVLWVQLLVYAMVLTT
                     IVSGADYFLNFRRRIEELARERHARAGTVADR"
     gene            complement(1940..2218)
                     /locus_tag="VHF88_00335"
     CDS             complement(1940..2218)
                     /locus_tag="VHF88_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160253.1"
                     /translation="MTYFTAVRIYSILELCLFAALMAVAIGGGSEHAKTILGWSHGVG
                     WLILCALVFYGATRGVFTWVTLALTVSPAGPLGSTAGIEFQARRDRSS"
     gene            complement(2215..2967)
                     /locus_tag="VHF88_00340"
     CDS             complement(2215..2967)
                     /locus_tag="VHF88_00340"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2160254.1"
                     /translation="MSDDEILLERDGGVATIVLNAPERRNALTRAMARRLVEVCEEVD
                     ADPGVGAVVVRGEGGFFCAGGDRQTLADAGRDPATPDAWVGIGDVYRSFQRVGELEPP
                     TVAAVRGGAVGAGMNLAFATDVRVVGRDAKLISGFLPIGLHPGGGHAALLGRTGAREA
                     ASALTLFGERIDGARAAELGLAWEAVDDAEVDARAQALAAAPGADPDLARRTARSLRT
                     ELGPPAVPWAAALELERASQMWSMRRKDLAGE"
     gene            complement(3004..3966)
                     /locus_tag="VHF88_00345"
     CDS             complement(3004..3966)
                     /locus_tag="VHF88_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2160255.1"
                     /translation="MASTARDRSADAPDGPTELDSGSKKGVLKRTFKEFQDDNLTDWA
                     AALTYYGVLSIFPALLALVSVIGLLGESTTQSLIDNVEAAAPGPARDIAVSAIENLQN
                     SQGAAGILFIAGLAAALWSASGYVAAFMRASNSIYDIGEGRPIYKTLPVRVGITLVLV
                     LLLAITAIAVAVTGPLAEEVGNLIGLGDTAVTVWDIAKWPVLFLIVSFMFAFLYWAAP
                     NVKQPGFRWISPGGMLGVGLWIVASALFALYVANFASYNETYGSLGGVIAFLVWLWIS
                     NLAILIGAEFNAELERGRRIESGDVGPGEEPFVEPRDTKKLDDK"
     gene            complement(4026..4289)
                     /locus_tag="VHF88_00350"
     CDS             complement(4026..4289)
                     /locus_tag="VHF88_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEX2160256.1"
                     /translation="MAKALYAPFGIIAGLLGGLVASSVFKQIWKRLAGADDPPTPRQS
                     EYSWKEILGAAALQGAIFSVVKAVINRGGAKGFQKLTGAWPGD"
     gene            4344..5039
                     /locus_tag="VHF88_00355"
     CDS             4344..5039
                     /locus_tag="VHF88_00355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2160257.1"
                     /translation="MMVPVILDIDGTLVDTNYHHALAWQRALRQHGETVPAWRIHRHI
                     GMGGDQIVTALVGEEFEQRCGDAVRETEGELYKQLIGEVGLLPGARGLLVELRARGVT
                     TVLASSAKADEVEVYLDMLDARELVDDWTTSADVEATKPEPDLVRAALDKAGSREAVL
                     VGDSVWDCRAAARAGVPTVGVLTGGFSREELLEAGAAVVYESAADVCAHLDEPPFGSA
                     VEAPRMSAAPPSS"
     gene            5081..6130
                     /locus_tag="VHF88_00360"
     CDS             5081..6130
                     /locus_tag="VHF88_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2160258.1"
                     /translation="MRIVVVGASGNVGTSVLEALAADTAVEEIVGVARRKPALTQAKT
                     TWVEADVVSSDLTNIFRGADCVIHLAWLIQPSRDLETLQAVNVEGSARVFHAVGTAGV
                     PALVYASSVGAYSPGPKDPPVDESWPTEGVMSSYYARHKAATERLLDRFELEQPGVRV
                     VRLRPGLIFKREAAAEIRRFFIGPLLPNPLVRRSLIPIVPRAKRLVFQAVHARDVGEA
                     YRLAAVSDVRGAFNVAADPVLDGEVLAEALGARPVSIHPGALRALADASWRLRLQPAA
                     PTWLDLAFGVPTMDTTRARTELGWTPRRRSDEALLELLEGLRDGAAHDTPPLEAQAGG
                     PLRVKELLTGVGGRQ"
     gene            6164..6793
                     /locus_tag="VHF88_00365"
     CDS             6164..6793
                     /locus_tag="VHF88_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX2160259.1"
                     /translation="MTEPMDATPFLPEPGATIDELARAAEDCRGCPLWRGATQAVLGE
                     GPLDARVVLVGEQPGDREDVEGRPFVGPAGQELDRALEEAGIDRDDAYVTNAVKHFKF
                     KERGKRRIHDKPTYSEVGACLPWLERELEQLRPQALVALGATAAQGLLGRDFRVTRQH
                     GQLVESELAPVVTATLHPSAVLRARSDEARREMHQTLVEDLTFVAKHLD"
     gene            6854..7180
                     /locus_tag="VHF88_00370"
     CDS             6854..7180
                     /locus_tag="VHF88_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160260.1"
                     /translation="MTDDREQRARDKPPTGFPEPPGEDPGDLTRHDDPHHTLNNPVGE
                     PDPTEWPDPYEQREDPRDPPDPDGQPFGENAHAPTGSTSTSEPHPSKDPEAIQTDAPE
                     RDNLDD"
     gene            complement(7212..8000)
                     /locus_tag="VHF88_00375"
     CDS             complement(7212..8000)
                     /locus_tag="VHF88_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2160261.1"
                     /translation="MTATTHVLGADPKERATIRVAAAGDIHCDGAKAAAAREAFAALD
                     GAADLVLLAGDLTTHGEPEQAAALADACRDLSVPVAAVLGNHDHHAGRAPELAAVLSE
                     AGIDVLDRRHAIYEVGGVQVGVAGAKGFVGGLHGLGLPDFGEPSLRAIYAETGAEAEA
                     LSHGLAQIALCPFRIALLHYAPLSDTLRGEPTEIWTFLGSDRLAAPLLEHRPDLVLHG
                     HAHAGTFEASLSGVPVHNVSVPVIGRDFWIFELTSADRAPGPLH"
     gene            8031..8645
                     /locus_tag="VHF88_00380"
     CDS             8031..8645
                     /locus_tag="VHF88_00380"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX2160262.1"
                     /translation="MASKEFEAIQETLKRTVALLRGAGVDFLLGGSLASWARGGPETS
                     HDLDLLLTHENAERALTALGEAGMRTERPVEGWLYKAWDGEVLVDLIFGPSGLEVDEV
                     IARGEDLPVLGMTLPTMALEDVLATKLLALSEHRIEYTAPLQTARALREQIDWRRLRE
                     QTRGSAYADAFFVLLEGLGVISDQSFAGAGVRVVDPPRPIRETG"
     gene            8650..9609
                     /locus_tag="VHF88_00385"
     CDS             8650..9609
                     /locus_tag="VHF88_00385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2160263.1"
                     /translation="MAKIGYALSSEEHTPADLVSYGRRAEEAGFDFALISDHFHPWLD
                     VQGHSPFVWTVLGALAQRTERLVLGTGVTCPTTRIHPAIVAHAAATTAALAEGRFWLG
                     VGTGENLNEHILGDHWPPTSVRREQLAEAVDVIRALWSGRQVEHYGRHYTVENARLYT
                     LPDRPPPIMVAASGTKAAELAGEIGDGLVALAPQSEIPDTFAASGGEGKPRIAQLHVC
                     WGEDEAEARQLAHEQWSNSGLGGELSQELPTPAHFEQAAQTVREEDVTGPLPCGPDVE
                     QYLDSAQQYLDAGFDHLYWHQIGPDQEGFIGFAERELLPRLQG"
     gene            9678..9953
                     /locus_tag="VHF88_00390"
     CDS             9678..9953
                     /locus_tag="VHF88_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2160264.1"
                     /translation="MQADPSDWLVALGDALREARQDAGMSQERLGLDAGIHRNYVGGV
                     ERGQRNPSVECIVTLANQLDVAPPDLFVKAGELLSRSRPASASASRK"
     gene            complement(9892..11001)
                     /locus_tag="VHF88_00395"
     CDS             complement(9892..11001)
                     /locus_tag="VHF88_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160265.1"
                     /translation="MSQPLRIALCAPTIAAVLAPAAAAQVTVGAPLNVPANVAEGCES
                     TVLPPLRNVIPVPATCTYWGMDSAGSWTSQTPRGGWRIVRARVRTGPRVGPMAFTVVR
                     AMRSQAGTVDNGGPAGIICCTTPFESQVFTPQPNTVNEVPVNLPVVNTVEVIDGEPVE
                     VVDYLGITLLSLGSSLPLHTAPGSSSIASYIAPGLRASGQALMGGSLPSAVVLVNADY
                     VGEGAGQPRTPLPPSNAFTLRPGVRLLAGGDAARLGLRVPGAGLLRASSPARAAVARA
                     NGARKRKRGKAERSGKPLLRPATRRVQRAGNARILLRLTRAGRKLRASRGRLRIRVRV
                     TFAPTGGTPATRTRRVTFAKPKPKRDGSGSTALRP"
     gene            11000..12619
                     /locus_tag="VHF88_00400"
     CDS             11000..12619
                     /locus_tag="VHF88_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2160266.1"
                     /translation="MRFDLLEAGEGAGGAAGQRRSKPLASSVARTSGISGSRFTRRQL
                     IGAAGVGATATAAAAAGWNGLAVNLDGRRNIVLIVVDTLRADHLGCYGNDSMHTPNID
                     ALAAGGMRFSRVFPEAMPTIPTRRSILTGRRAFPFRDWEPWAGLGTTPGWRPIEPGVP
                     TLVTELGRRGYRTACVTDNPFLGFASTYRPFRRSFDRFAAVHGQTPRGDPPPDISIEE
                     ARRLIPRSRWDDLRHIDDMRRYLAANGGRGGDVDESSTATGRVFASARWLARRMLTKR
                     PFLLMVDAFDPHEPWAAPSSYADLYREPGDDGLPPMGDVGYTPVEELTPTELQRVRIA
                     YRATLSMVDNQIGKLLDTLDRLGLANNTVVMLVSDHGFHLGERDWVGKSAWRLTPELT
                     RVPLIVRDPDGRGAGVASDWFADTHDLAPTLLSLSGARPSTAFAGADLSPIADGGLPD
                     EPRDFTYGGYSNHSYVRDDDWAMVTNNERTGAQLYDLHADPDERVDIASSRPEVVERL
                     YERMVADIGAPPPYYSAAELARTPRRAPASW"
     gene            12755..14005
                     /locus_tag="VHF88_00405"
     CDS             12755..14005
                     /locus_tag="VHF88_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX2160267.1"
                     /translation="MLLAIAIFVAALALIASERVDRTKVALLGATLIVLTQTIDQEHA
                     IEAVDWNTIGLLAGMMLMVKLTEATGVYTWVAIRAGQLSKGRPLAVVVSLAVTTGVLS
                     AFLDNVTTVLLIVPITFLLADTLDVDPVPLIVIEIIASNIGGTATLIGDPPNILIAGH
                     TGLSFGAFIVNLAPIVVVALAVVIPGLYLVFRSRLRIAPESRARMMELDADRSIEDAA
                     EAKRTVPILVVTILLFFAHKALHIEPATVALAGASVMLLVTRQSVEEALSGIEWSTLF
                     FLIGLFVMVGALEATGALDEVTDGIASVTDGNRTAELLGIVWVSAIGSGIVDNIPFTA
                     AMIPVVDSLGGATDDAYWWALALGACFGGNATIVAAAANVAASGMAANAGRPISFLYF
                     LKYGLGATAVSLILATAYVMVRYA"
     gene            14002..14460
                     /locus_tag="VHF88_00410"
     CDS             14002..14460
                     /locus_tag="VHF88_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2160268.1"
                     /translation="MTPRAISDVVQREAPLLRTDETIEQATRTLLAGDLPALPVVDPD
                     DGFAGIYGEREFMTAVFPGYLKQLRYAGFVTQSLDDALERNETCRGEAVSKHMNTEHV
                     DVGPDFADLQIAETFLHHRVLVVPVVDHGTVCGVITRSDFFRAVAGRFLG"
     gene            complement(14492..15268)
                     /locus_tag="VHF88_00415"
     CDS             complement(14492..15268)
                     /locus_tag="VHF88_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX2160269.1"
                     /translation="MDAHAGEADRREGGRRTKRVRRRCAELRHGDDDAFERWAAGATE
                     RLDPAMAWLGVLFALLVGYELAVDLSPSAARALEIAGWTIWAVFLAEFAVKLWLAPRR
                     ARFLRRNWIQAVALLVPTLRLLRFLRLVRLGRALPGARVLSSSYRSVGSARALFGSRL
                     GYLGAIGVVVTVAAAQLAFLFERDVSEPAFDSFGDAILWASAVVLALQGDPVPASTGG
                     RIVMLAGFAFGLVLIASLAGTVGAFLIEGRRERPGADDSQ"
     gene            15368..16090
                     /locus_tag="VHF88_00420"
     CDS             15368..16090
                     /locus_tag="VHF88_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160270.1"
                     /translation="MTLLAVCVLAATHVLAGKLRFLEGVPRSRWLSFAGGVSVAYVVV
                     RILPELSAGQREVDHPANALLPFLEGHVYLLTLAGLVAFYGVERSSSSSRARRREASG
                     IDATDRSAFVVSIGGFAVYNVIVGYVLADFDATREHSLAALAAALAVHFLVNDYGLRE
                     HHKDAYRDVGRWVLAAAVLVGWAIGRSAELDEAGVALLVAFLAGGIILNTFKEELPRD
                     RESRFLPFVAGAAGYAALLQLV"
     gene            16132..17490
                     /locus_tag="VHF88_00425"
     CDS             16132..17490
                     /locus_tag="VHF88_00425"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEX2160271.1"
                     /translation="MLNRRKERPLLERVRNTFWALPALGIAVAIGAALALPVLDDTIT
                     LPDAVSFSGSADTARATLQMIATVAVSVAGISFSVIVVALVLASQQLSPRVMRSFQRH
                     PLNQAVLGLFLATAAFSLFVLGSIDDSSDEVPEVAVTVAMVLAALSLATFVVFLHHMV
                     RSLNASAVIRRIAAEGHEAVHTPYPEYVGEAPGDAEAAERDARAIVERSVSREVRAPR
                     AGYLASVKADDVLATADDCDGFVEQRQAIGDFTVTGGLLAVVWCSRESLDELAERVET
                     AFVLNEERLVDGDVAFPLRQLADVALKGLSPGINDPTTAENAMDSVTDSLVRLARQPE
                     PALLRLAPSGTPRLRATTPSLDALIKLGFDQVRRDGAAQPSFAVRLLELLAALRDVGG
                     ARAAGSEELDRQARAVRDHAIALADIPADVELLRRSYDRLHGSAAFGDGRGSLPRDVV
                     AR"
     gene            complement(17500..18051)
                     /locus_tag="VHF88_00430"
     CDS             complement(17500..18051)
                     /locus_tag="VHF88_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX2160272.1"
                     /translation="MATTVPILTTVGHSTHEPDRLVELLRGASVEAIADVRRFPGSRR
                     MPWFAAEAMREWLADAGIGYEHLPELGGRRKPVPDSPNGGWEVRQFQGYADHMESAEF
                     EAGLDRLLALADERSVAAMCAEAPWWRCHRRLLADALLVRGHAVVHLMPDGRTQRHEL
                     TPFAVVDGTYLTYPPLQDELALD"
     gene            18151..19290
                     /locus_tag="VHF88_00435"
     CDS             18151..19290
                     /locus_tag="VHF88_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2160273.1"
                     /translation="MKRRVLVLGAGFGGLELATRLSETVADAVDVTLLDRNDSFYFGF
                     SKLDVMLGREGADAVRLPYGDIAKDGVEFRQETVTAIDPEARRVATDVGTYEADFLVV
                     AMGADYDMAATPGFEEGGHEYYTLTGAERLRDALADFDGGRVLVSVLGQPFKCPPAPF
                     EGTFLLDEHFSEQGIRDAVEMAIAFPMQRPVPVTGEVSQMFRDGLATRGIEELPQHLV
                     TSLDPAAGVARLATGKTLPYDLFVGIPVHRAPDPLPASGLAVDGWVPVDQANLRTRFP
                     QVYALGDVCTGPRTVPKAGIFAESAALVVADDIAAKVSGAEPPPPYEASGVCYAEFGD
                     GLVGKVEINFLRGDAPAAQRHDPSREQAAEKADFGATRRARWFGA"
     gene            19480..19653
                     /locus_tag="VHF88_00440"
     CDS             19480..19653
                     /locus_tag="VHF88_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160274.1"
                     /translation="MVHDSSSQPSSATLSASQLIPLLRAIADPEGDGTISPQDAASIE
                     RVETNLTRFLGLG"
     gene            complement(19666..20058)
                     /locus_tag="VHF88_00445"
     CDS             complement(19666..20058)
                     /locus_tag="VHF88_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2160275.1"
                     /translation="MADGPGAVLDASAVIALLWEESGSDAVEGLLGAAVISAVNWSEV
                     LQRYNALGLDLTGRRDSLEALGVSIEDFSGDDAEIAASMWASTRTAGLSLADRACLSL
                     ARRLGLPVHTADRAWGTVEVGVEVVLIR"
     gene            complement(20051..20326)
                     /locus_tag="VHF88_00450"
     CDS             complement(20051..20326)
                     /locus_tag="VHF88_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX2160276.1"
                     /translation="MARRARAVPLRIGPQGRVVIPAEMRRTLEIQPGETLMAHVEEDR
                     LVIERREQILERLRGELRGTTPAGTSMVNELIAERRAEARREAAEHG"
     gene            complement(20768..21346)
                     /locus_tag="VHF88_00455"
     CDS             complement(20768..21346)
                     /locus_tag="VHF88_00455"
                     /inference="COORDINATES: protein motif:HMM:NF037501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HEX2160277.1"
                     /translation="MTGQPSLYDFAFRGLMAEQALDHGGRPKAALPFLDEEVAEKVSL
                     DLLDDHFVAQARRMATVYTAIAAFENTVRDLVRRVLVEQEGAEWWDTCVSNNIQGKAK
                     RRQEDEAKHRFHTQRGDDPVSFIDFGDLLNVMRANEEAFAPFWPSPEWAKSIFDAVER
                     SRNVIMHSGFLGVEDVERVGMMVRDWVRQVGA"
     gene            complement(21343..21960)
                     /locus_tag="VHF88_00460"
     CDS             complement(21343..21960)
                     /locus_tag="VHF88_00460"
                     /inference="COORDINATES: protein motif:HMM:NF036563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5343 domain-containing protein"
                     /protein_id="HEX2160278.1"
                     /translation="MTARNVEPLFNALRNAKAPPKFTQRFLEELDFKSTNDRLYINVF
                     KAIGLLSEDGSPTARYFEFMDQTQSRRVLAEALEEAYADLFQVRRDAQNMERQELRGK
                     IKTLTQGQVSDNVVDKMAMTFLALAEQADFDAPRPNQTEATEEAEISAPDDQRDVPPI
                     DLGGSSAGAIKLGGFVYNVELHLPDSRDPAVYEALFRALKTHLLS"
     gene            complement(22272..22502)
                     /locus_tag="VHF88_00465"
     CDS             complement(22272..22502)
                     /locus_tag="VHF88_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX2160279.1"
                     /translation="MAASIDFTIAFEPPDEDGWIVARVLEVPGAMSQGRTRDEARENA
                     LDALSTVLTPDEELAGGAPQADREHLRFTAAA"
     gene            22808..23083
                     /locus_tag="VHF88_00470"
     CDS             22808..23083
                     /locus_tag="VHF88_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2160280.1"
                     /translation="MPEIGAHCVSSFKPMSPPDISPTDPAATASFLSEPLLDSDAAAA
                     LLSVRRSWIYEAVRDGRLPHVKVGRHIRFLRADLESWVFAQRRGRLF"
     gene            complement(23080..23304)
                     /locus_tag="VHF88_00475"
     CDS             complement(23080..23304)
                     /locus_tag="VHF88_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160281.1"
                     /translation="MADEEAQTDQAVLGLLLTDSAAIWSVEEMASEIGNPIHVTDSIN
                     RLAAIGLVHRLDGFLFATRAAAKATELALA"
     gene            23472..23708
                     /locus_tag="VHF88_00480"
     CDS             23472..23708
                     /locus_tag="VHF88_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2160282.1"
                     /translation="MGRPINPQPALGEAIRQLRTKRGISQEDLAHLAGVTTGTLSVIE
                     RGRSNPAWGTVKAIAKALDVSMPELAKLAEKHER"
     gene            complement(23716..24846)
                     /locus_tag="VHF88_00485"
     CDS             complement(23716..24846)
                     /locus_tag="VHF88_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2160283.1"
                     /translation="MQSQLSRPVSGHVFRVERQRGPVWYAKYRLPDGRQVQKKLGPAW
                     TKRGRPSDGYFTKQTAQAQLDQIIDEIRRGQHAGMVRTGATFQQAADEYLRYIEEDRA
                     RKPSTVAGYRALVRAQLLPAFGKLPVESITTPMVESWIAGLEVAPATRTKAYVLMGGI
                     MKRARKVYGLPFNPVTDVEKPPAPTASGDIEVFSPEEVMALVDAAASEQDAAIYLTAA
                     FSGLRRGELIALRWRDIDIEGSTIRVRASYSDGALATPKSGKVRSVPMAPNVAETLAN
                     LRQRPLWTAEDDFVFVGRTGSFLDGRALRRRYQDALDRAGLRRLRFHDLRHTFGTRVI
                     AKADIRRVQEWMGHADVKTTMRYLHYAPRKDDAALVASAFDA"
     gene            complement(24982..25054)
                     /locus_tag="VHF88_00490"
     tRNA            complement(24982..25054)
                     /locus_tag="VHF88_00490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25019..25021),aa:Thr,seq:cgt)
     gene            complement(25096..26790)
                     /locus_tag="VHF88_00495"
     CDS             complement(25096..26790)
                     /locus_tag="VHF88_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX2160284.1"
                     /translation="MSRNVAEAVVDELVDAGVEVVFGLPGVHNLALFAALDASPIRTI
                     VVRHEAAAAYAADAVGRLTGRPGVCVTTSGPGAANAITAMGEAQASRSPLLHITTTVA
                     RRHLDPERSRGVLHEHPRQRDLFVPVSKVALHAYSGAGAARDIRDALRAAEAAPRAPA
                     YVELPTDLLDDEAGPAGVSGDGSRRASGLSANGLDRADPPEPASSELRALADRLAAAE
                     RPAIWVGSGAAACGAAIVALAERLDAPVVLTHSAKRRWNAAPHPLVPAYPPHEPPIGE
                     LLGECDTLLVLGSDLDGMMTQEFRLPLEPILRVDVDPQRAAGDDRYASAQAVVADAAE
                     VVPALLDLVPERPGGWGPEAVVAADVAARQALADDPGASGGLAYLSALDAALGDRDAA
                     LVCDMAVSAYWTGGYLPLRPGRRILYPLGWGTLGFALPASIGAATAHSGRTLAICGDG
                     GVLFAIGELATLAQERLDVTVVVHNDACYGMLRYDEERHFGRTFAVDLQAPDFAALAR
                     SFGVPAWNADLAAPDDLQSALTAALATDGPSLVEVTGELSPPRVTSARWPLVHAAA"
     gene            complement(26787..>27943)
                     /locus_tag="VHF88_00500"
     CDS             complement(26787..>27943)
                     /locus_tag="VHF88_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2160285.1"
                     /translation="AHAEALRSVALLRYFAGETAQPLGEVYPSGVADRHLYTERVPLG
                     VVGVITPWNFPLAIPVWKIAPALAFGNAVVWKPSQLTPRCAVNLVELLHEAGLPAGVL
                     NLVTGGRSPVGEALVSAPQLDGISFTGSTAVGRAIQRDVVERGVKVQLELGGKNPVIV
                     MDDADVDLAVRETVRGAMLSTGQKCTATSRAIVVGDPGRFTEALLPAVSALRVGDPSA
                     PDTDVGPLASQGQYETVLSYLDVAREEGHELAAGGEPIDLDGGYFVAPTVYTGVGQDS
                     RIAREEVFGPVLGVIPASSIEDAVRIANDVDYGLSASIFTRDVGRAFEFAREVEAGVV
                     HVNSETAGSEPQVPFGGMKDSSSHSREQGKAARDFYTDLKTVYLDMPAPR"
BASE COUNT         4149 a         9628 c        10126 g         4040 t
ORIGIN      
        1 ggtcgtcaac gaggcgatcg acgcggtcgt gctcggcgag gcgttcaagg ccgagacgct
       61 cgcccagcac atcgcagagc gcgtgcgcga ccgtcaggag gcgctgcgcg ccgaggtgaa
      121 gatcgccgcc cgctatcccg agcgccagaa cacgcccgcc tcgggctccg ccacccagga
      181 gatctacacc gtgttcggcg ttgcggtcgc gtccgagcgc ggcacgcggc ggctgatcgg
      241 cgttgaggcg cacggcatga ccgcatgtcc ctgcgcccag gagatggtcg ccgaccgctc
      301 gcgcgagcgg ctgctcgccg acggcttcac cgacgacgag atcgagcgcg tgctggcggc
      361 ggttcccgtg gcgacgcaca accagcgcgg catcggccgg ctctacgtcg gctgcgtcga
      421 gggctgcacc aacaccgtcg aggcgcggac cctgctgcgg atcgtcgagg gatcgatgag
      481 ctcggagatc tacgagctga tgaagcgcag cgatgaggtc gaggtcgtcg agcgcgcgca
      541 catgcacccc cgcttcgtgg aggactgcgt gcgcgagatg gtgcggaccg ccgttgacga
      601 gttcgccggc ttcgacggcg acacgttcct gctcgcgcgc caggagaacc tcgagacgat
      661 ccaccgccac agcgtggtcg ccgagcgcca cgggctgctc ggcaggctgc ggcaggagct
      721 gaccgacgag gtccacctgc agcgccacgt caccatgcgc gagtggctcg agggccccgc
      781 cgccttcgcc gactgagcct cccccatacg gccagacgcg tcgcggcggt tcgatcgtcc
      841 gggcgaggct gccgacaggt gagcgaggct ccatgacgat cgcccaccgc atcccgctgt
      901 ccgaggacac cgcgctggtc acggcgctgg ccggtacggc gatggcgttc tcgcactgcg
      961 ccgaggacga ggccgagcgc tggctgcgcg cgctgcggct gcacggccag gtcggctgcg
     1021 cgctgcaggc gctcggcgtc ggcgaggcgc cgctcgagag ccgcgccgcc gagggcccgg
     1081 acggcgctgg aaaggtttgc gacgagccgt ctgcgtcgcc gccgctcggc gatgccgcgc
     1141 tcgaccggac cgtgcgcgag gccgagcgcc gggcgatcga acgcaacgcc gaggcggtcg
     1201 ggaccgcgga cctgctgctc gccctgctcg acgtctacgg cgagccgatg gaccgggcgc
     1261 tcgaggcgcg cggcgcgacc cgcgacgagg tgatcgagcg gctcgccgcg atgggcgacc
     1321 accccgagct ggcctccggc tgagccggtc gcgtcagcgg tcggccacgg tgccggcgcg
     1381 ggcatggcgc tcgcgggcca gctcctcgat ccggcggcgg aagttgagga agtagtcggc
     1441 gccggagacg atcgtcgtca ggaccatcgc gtagacgagc agctggaccc acaggacgtc
     1501 cgggtccgcg accgcgatca gcgcgagcac cgccgccgac tggacgatcg tcttgacctt
     1561 cccgagggcg ctcgccggga tcacgacgcc ctgctgaccg gccgcgatgc gcagccccga
     1621 gaccgcgaac tcgcgcgcga tgacgatcat cgccacccac gcgtcgacgc ggtcgaggct
     1681 gaccagcgtc agcagcgcgg cggcgatcag cagcttgtcg gcgacggggt ccatcacctt
     1741 gccgaacgtc gtgatcgact ggcgcgagcg ggcgatgtag ccgtcgaggc cgtcggtcag
     1801 cgaggcgagc acgaacaccg cggccgctag ctcggagccg ccctgggcct ccgttccgag
     1861 cgcgacgacg agcaccggca cgagcaggat gcgcagcagc gtgaggacgt tgggcaggtt
     1921 gagcgggaac atcgctcgct cagcttgacc gatcgcggcg cgcctggaac tcgatgcctg
     1981 ccgtggagcc gagcgggccg gctggggaga cggtgagcgc gagggtgacc caggtgaaga
     2041 cgccgcgggt cgcgccgtag aagacgagcg cacagaggat cagccagcca acgccatggg
     2101 accagccgag gatcgtcttc gcgtgctcgc tgccgccgcc gatcgcgacg gccatcaggg
     2161 cggcaaagag gcagagctcg aggatcgagt agatgcgaac ggcggtgaag taggtcactc
     2221 gccggcgagg tccttgcggc gcatcgacca catctgggac gcgcgctcga gctcgagagc
     2281 ggcggcccac ggaaccgccg ggggaccgag ctcggtgcgc agcgagcggg ccgtccgccg
     2341 ggcaaggtcg gggtcggcgc cgggggcggc ggcgagcgcc tgggcgcgcg cgtctacctc
     2401 ggcgtcgtcg acggcctccc aggcgaggcc gagctcggcg gcgcgggcgc cgtcgatccg
     2461 ctcgccgaag agcgtcaggg cggaggccgc ctcgcgggcg ccggtgcgtc cgagcagcgc
     2521 ggcgtggccg ccgccgggat ggaggccgat cggcaggaag ccggagatca gcttggcgtc
     2581 gcggccgacg acgcggacgt cggtcgcgaa ggcgaggttc atgccggcgc cgaccgcgcc
     2641 gccgcggacc gcggcgaccg tcggcggctc cagctcgccg acgcgttgga aggagcggta
     2701 gacgtcgccg atgccgaccc aggcgtcggg cgtggcgggg tcgcggccag cgtcggcgag
     2761 tgtctggcgg tcgccgccgg cgcagaagaa gccgccctcg ccgcggacga cgacggcgcc
     2821 gactccgggg tcggcgtcga cctcctcgca gacctcgacc aacctgcgcg ccatcgcccg
     2881 ggtcagcgcg ttgcggcgct cgggtgcgtt gaggacgatc gtcgcgacgc cgccgtcgcg
     2941 ctcaagcagg atctcgtcgt cggacatgcg cggggacgct atccggccgg tggccgaagg
     3001 cgcctacttg tcgtccagct tcttagtgtc gcggggctcg acgaacggct cctcgccggg
     3061 cccgacgtcg ccggactcga tccgtcggcc gcgctcgagc tcggcgttga actcggcgcc
     3121 gatcaggatc gcgaggttgg agatccagag ccagacgagg aaggcgatca cgccgccgag
     3181 cgagccgtag gtctcgttgt aggaggcaaa gttcgcgacg tagagcgcga aaagcgccga
     3241 ggcgacgatc cagaggccga cgccgagcat gccgcccggg ctgatccagc ggaagccggg
     3301 ctgcttgacg ttcggcgccg cccagtagag gaaggcgaac atgaagctga cgatcaggaa
     3361 gaggaccggc cacttggcga tgtcccagac cgtcacggcg gtgtcgccga gcccgatcag
     3421 gttgccgacc tcctcggcga gcggaccggt gacggcgacc gcgatcgcgg tgatcgcgag
     3481 caggaggacg aggacgaggg tgatgccgac gcggacgggg agtgtcttgt agatcggccg
     3541 cccctcgccg atgtcgtaga tcgagttcga cgcccgcatg aaggcggcta cgtagccgga
     3601 ggccgaccac agagcggcgg cgaggccggc gatgaagagg atgcctgcgg cgccctggct
     3661 gttctggagg ttctcgattg cgctgacggc gatgtcgcgt gcggggccgg gcgccgccgc
     3721 ctcgacgttg tcgatcagcg actgcgtcgt cgactcgccc agcagtccga tgaccgagac
     3781 gagcgcgagc agcgccggga agatcgagag gacgccgtag taggtgagcg cggcggccca
     3841 gtcggtcagg ttgtcgtcct ggaactcctt gaacgtgcgc ttgagcacgc ccttcttcga
     3901 gccgctgtcg agctctgtcg gcccgtcggg cgcgtcggcg gaccggtctc gagccgtcga
     3961 agccatggac gtgggatacc acgcgcccgg cgatcgaaac cagcgctggc gctggcgccg
     4021 gtgcgtcagt cgcccggcca ggccccggtc agcttctgga agcccttggc gccgccgcgg
     4081 ttgatgaccg ccttgacgac cgagaagatc gcgccctgga gcgcggctgc tccgaggatc
     4141 tccttccagg agtactcgct ctggcgcggg gtcggcgggt cgtccgctcc ggcgagccgc
     4201 ttccagatct gcttgaagac actgctcgcg accaggccgc cgagcaggcc ggcgatgatc
     4261 ccgaacgggg cgtagagagc cttggccatc gtgatcgatg gtacgggatt gagcggcgtg
     4321 cgaacgggta gaacgccggc tcgatgatgg tcccggtgat cctcgacatc gacggcacgc
     4381 tcgtcgacac gaactaccac cacgcgctcg cctggcagcg cgcgctgcgc cagcacggcg
     4441 agaccgtgcc ggcgtggcgg atccatcgcc atatcgggat gggcggcgac cagatcgtca
     4501 ccgcgctcgt cggcgaggag ttcgagcagc gctgcggcga cgcggttcgc gagaccgagg
     4561 gcgagctcta caagcagctg atcggcgagg tcggtctgct ccccggcgcg cgcgggctgc
     4621 tcgtcgagct gcgcgcgcgt ggcgtcacga cggtgcttgc gagctccgcc aaggccgacg
     4681 aggtcgaggt ctacctcgac atgctcgacg cccgcgagct ggtcgacgac tggacgacct
     4741 cggccgacgt cgaggcgacc aagcccgagc ccgacctcgt gcgcgcggcg ctcgacaagg
     4801 cgggaagccg cgaggccgtg ctggttggcg actccgtctg ggactgtcgt gccgcggccc
     4861 gggcgggcgt gccgacggtc ggcgtcctga ccggcgggtt ctcgcgcgag gagctgctcg
     4921 aggccggcgc ggcggtcgtc tacgagtcgg cggccgacgt ctgcgcgcac ctcgacgagc
     4981 cgccgttcgg cagcgcggtc gaggcgccgc gcatgtcggc ggcgccgcct tcgagctgag
     5041 cgagcggttt gccggcgtag gcccggggta ggtcgctggc gtgcggatcg tcgtcgtcgg
     5101 agctagcggg aacgtcggca ccagcgtgct cgaggcgctg gcggccgaca ccgcggtcga
     5161 ggagatcgtc ggggtcgccc gccgcaagcc ggcgctgacc caggcgaaga cgacgtgggt
     5221 cgaggccgac gtggtcagct cggacctgac caacatcttc cgcggcgccg actgcgtgat
     5281 ccatctcgcc tggctgatcc agccgtcgcg cgacctcgag acgctgcagg cggtcaacgt
     5341 cgagggcagc gcacgcgtct tccatgcggt cgggacggcg ggcgtgccgg cgctcgtata
     5401 cgcgtcctcg gtgggcgcct actcgcccgg gccgaaggat ccgccggtcg acgagagctg
     5461 gccgaccgag ggcgtaatga gctcttacta cgcgcgccac aaggccgcga ccgagcgact
     5521 gctcgaccgc ttcgagctcg agcagcccgg cgtcagggtg gtgcggctgc ggccgggact
     5581 gatcttcaag cgcgaggcgg cggccgagat ccgccggttc ttcatcgggc cgctgctgcc
     5641 gaacccgctc gtgcggcgct cgctgatccc gatcgtgccg cgcgcgaagc ggctcgtctt
     5701 ccaggccgtc cacgcgcgcg acgtcggcga ggcctacagg ctggcggccg taagcgacgt
     5761 gcgcggagcg ttcaacgtgg ccgccgaccc ggtgctcgac ggcgaggtgc tcgccgaggc
     5821 gctcggcgcg cggcccgtgt caatccaccc gggggcgctg cgcgcgctcg ccgacgcgtc
     5881 atggcggctg cgcctccagc cggcggcgcc gacgtggctg gacctcgcgt tcggcgttcc
     5941 gacgatggac accacccgcg cgcgcaccga gctcggctgg acgccgcggc ggcgctccga
     6001 cgaggcgctg ctcgagctgc tcgaaggcct gcgcgacggc gccgcccacg acacgccgcc
     6061 gctcgaggcg caggccggcg gcccgctgcg agtgaaggag ctgctgaccg gggtcggagg
     6121 gcggcagtga ccgcgacgcc ggcctggggg tgacgcggtg acggtgaccg agccgatgga
     6181 tgcgacgccg ttcctgcccg agccgggcgc gacgatcgac gagctggcgc gggcggccga
     6241 ggactgccgc ggctgcccgc tctggcgcgg agcgacccag gcggtgctcg gcgagggtcc
     6301 gctcgatgcg cgcgtggtgc tcgtcggcga gcagcccggc gatcgcgagg acgtcgaggg
     6361 gcgtccgttc gtcggaccgg ccggccagga gctcgaccgg gcgctcgagg aggcgggcat
     6421 cgaccgtgac gatgcctacg tcaccaacgc ggtcaagcac ttcaagttca aggagcgcgg
     6481 caagcggcgg atccacgaca agccgaccta cagcgaggtc ggggcctgcc tgccctggct
     6541 ggagcgcgag ctcgagcagc tgcggccgca ggcgctcgtc gcgctcggcg cgacggcggc
     6601 gcagggcctg ctcggacggg acttccgcgt cacccgccag cacgggcagc tggtcgagtc
     6661 ggagctggcg ccggtggtga ccgcgacgct gcacccgtcg gccgtcctgc gcgcgcgcag
     6721 cgacgaggcg cggcgcgaga tgcaccagac gctggtcgag gacctgacct tcgtggcgaa
     6781 gcacctcgac tgaggcgacg ctcgcggcgt gcggcgaacg tttcgcgcca tcggcgctgg
     6841 gtacggcttg gacatgaccg acgatcgaga gcagcgagcc cgcgacaagc cgccgaccgg
     6901 ctttcccgag cctcctggcg aggatcccgg cgacctgacg cgccacgacg atccacacca
     6961 cacgctcaac aaccccgtcg gcgagccgga cccgacggag tggcccgatc cctacgagca
     7021 gcgcgaggac ccacgcgacc cgccggaccc cgacggacag ccgttcggcg agaacgcgca
     7081 cgctccgacc gggagcacca gcactagcga gccgcacccg tcgaaggacc cggaggcgat
     7141 ccagacggac gcgccggagc gcgacaacct cgacgactga ggttcgctcg cgctcgacgc
     7201 cgcggccgcg ctcagtgcaa cgggccgggc gcgcggtcgg cgctggtcag ctcgaagatc
     7261 cagaagtcgc ggccgatcac cggcaccgag acgttgtgca ccgggacgcc gctgagcgag
     7321 gcctcgaacg tgccggcatg ggcgtgcccg tgcaacacga ggtcgggacg gtgctcgagc
     7381 agcggcgccg cgagacggtc gctgccgagg aacgtccaga tctcggtcgg ctcgccgcgc
     7441 agcgtgtcgg aaagcggcgc gtagtgcagc agcgcgatcc ggaacgggca cagcgcgatc
     7501 tgggcgaggc cgtgcgagag cgcctcggcc tccgccccgg tctcggcgta gatcgcgcgc
     7561 agcgacggct cgccgaaatc gggcaggccg aggccgtgga ggccgccgac gaagcccttc
     7621 gcgcccgcca cgccgacttg gacaccgccg acctcgtaga tcgcgtgcct gcggtcgagg
     7681 acgtcgatcc cggcctccga gaggacggcg gcaagctccg gcgcgcgacc ggcgtggtgg
     7741 tcgtggttgc cgagcacggc ggcgaccggg acgctgagat cgcggcaggc gtcggccagc
     7801 gccgcggcct gctcgggctc cccgtgcgtg gtgaggtcgc ccgcgagcag gacgaggtcg
     7861 gcggcgccgt cgagcgccgc gaaggcctcg cgtgcggcgg ccgccttggc gccgtcgcag
     7921 tggatgtcgc cggcggccgc gaccctgatc gtcgcgcgct ccttggggtc cgccccgagg
     7981 acgtgcgtgg tcgcggtcat ggaatcccca tacccgggta aggggcatcc atggcgagca
     8041 aggagttcga ggcgatccag gagacgctca agcggaccgt cgcgctgctg cgcggcgcgg
     8101 gcgtcgactt cctgctcggg ggcagcctcg cgagctgggc gcgcggcggg cccgagacca
     8161 gccacgacct cgacctgctg ctcacccacg agaacgccga gcgggcgttg accgcgctgg
     8221 gcgaggcggg gatgcggacc gagcgaccgg tcgagggctg gctctacaag gcctgggacg
     8281 gcgaggtcct ggtcgacctc atcttcggcc cgtcgggcct cgaggtcgac gaggtgatcg
     8341 cccgcggcga ggacctgccg gtgctcggca tgacgctgcc gacgatggcg ctcgaggacg
     8401 tgctcgccac caagctgctc gccctgagcg agcaccgaat cgagtacacg gccccgctgc
     8461 agaccgcgcg ggcgctgcgc gagcagatcg actggcgccg gctgcgcgag cagacgcgcg
     8521 gctccgccta cgccgacgcg ttcttcgtgc tgctcgaggg gctcggcgtg atctcggacc
     8581 agagcttcgc gggagccggc gtgcgcgtcg tcgacccgcc gcggccgatc agggagacgg
     8641 gatagccgca tggcgaagat cggctacgcg ctgtcgagcg aggagcacac ccccgccgac
     8701 ctcgtgtcat acgggcgccg ggcggaggag gcgggcttcg acttcgcgct gatctcggat
     8761 cacttccacc cgtggctgga cgtccagggc cactcgccgt tcgtctggac ggtgctcgga
     8821 gcgctggcgc agcggaccga gcggctggtg ctcgggaccg gcgtcacctg tccgacgacc
     8881 cgcatccatc ccgcgatcgt cgcgcacgct gcggctacga cggccgcgct ggccgagggg
     8941 cggttctggc tcggcgtcgg caccggcgag aacctcaacg agcacatcct cggagaccac
     9001 tggccgccga cgtcggtccg ccgcgagcag ctcgccgagg cggtcgatgt gatccgcgcg
     9061 ctctggagcg gccgtcaggt cgagcactac ggtcggcact acacagtcga gaacgcgcgc
     9121 ctctacacgc tgcccgacag gccgccgccg atcatggtcg cggcgtcggg gaccaaggcg
     9181 gccgagctgg ccggcgagat cggcgacgga ttggtcgcac tggcgccgca atcggagatc
     9241 ccggacacct tcgccgcgag cggcggcgag ggcaagccgc ggatcgccca gctgcacgtc
     9301 tgctggggcg aggacgaggc cgaggcgcgc cagctcgctc acgagcagtg gtcgaacagc
     9361 gggctcggcg gcgagctctc gcaggagctg ccgacgccgg cccacttcga gcaggcggcc
     9421 cagaccgtgc gcgaggagga tgtgaccggg ccgcttccgt gcggaccgga tgtggagcag
     9481 tacctcgact ctgcgcagca gtacctggac gccggcttcg accacctcta ctggcaccag
     9541 atcgggccgg accaggaggg gttcatcggc ttcgcggagc gcgagctgct gccgcggctg
     9601 caggggtgac agccgcccga gaaagtcaca cgatcaggct gtatacataa cacggtaggg
     9661 ctgggacggt tcggcgtgtg caggccgacc cgtccgactg gctcgtcgca ttgggcgacg
     9721 cgctgcgtga ggcgcgccag gatgccggca tgagtcagga gcggctcggg ctcgacgctg
     9781 gcatccaccg caactacgtc ggcggcgtcg agcgcggcca gcgcaacccg tcggtcgagt
     9841 gcatcgtgac gctcgccaac cagctcgacg tcgcgccgcc cgacctgttc gtcaaggccg
     9901 gagagctgtt gagccgctcc cgtcccgctt cggcttcggc ttcgcgaaag tgacgcggcg
     9961 cgttctggtc gcgggcgtgc cgcccgtcgg cgcgaacgtc acgcggaccc gaatgcggag
    10021 acgaccgcgg ctggctcgga gcttgcggcc ggcacgggtc agccgcagca agatccttgc
    10081 gttgccggcg cgctgcacgc gtcgcgtcgc cggacgcagc aggggcttcc cgctccgctc
    10141 cgccttgccg cgcttgcgct tccgggcccc gttcgctcgc gcgaccgccg cccgcgccgg
    10201 cgagctggct cgcagcagcc ccgcgcccgg cacgcggagc ccaaggcggg ctgcgtcgcc
    10261 gccagcgagc agccgaactc ccggacgcag cgtgaaggcg ttgctcggcg gaagcggggt
    10321 ccgcggctgg ccggcgccct cgccgacgta gtcggcgttg acgaggacga cggcgctggg
    10381 cagagagccg cccatcaacg cttggccact cgcacgcagc ccgggcgcga tgtacgaggc
    10441 gatcgacgac gatcccggag cggtgtgcaa gggcaggctg ctgccgaggc tcaacagcgt
    10501 gatgccgaga tagtcgacga cctcgaccgg ctcgccgtcg atcacctcga ccgtgttgac
    10561 gaccggaagg ttcaccggca cctcattgac ggtgttcggc tgcggtgtga agacctggct
    10621 ctcgaacggt gtcgtgcagc agatgatccc ggccggcccg ccgttgtcca cggtgccggc
    10681 ctgtgaccgc atcgcgcgca cgaccgtgaa cgccatcggg ccgacccgcg gaccggtgcg
    10741 gacccgcgcc cggacgatcc tccagccgcc gcgcggagtc tgcgacgtcc aggaccctgc
    10801 actgtccatt ccccagtagg tgcacgtcgc cgggaccgga atgacgttcc gcagcggggg
    10861 caggactgtg ctctcgcacc cctcggcgac gttcgcgggg acgttcaggg gcgcgccgac
    10921 cgtcacctgg gcggcggcgg caggggcgag cacggcggcg atcgtcggcg cgcagagcgc
    10981 gatccgcagc ggctgggaca tgcgattcga cctcctcgag gcgggagagg gagctggcgg
    11041 agcggcgggg cagcgccgaa gtaaaccact cgccagctcg gtcgctagaa ctagcggcat
    11101 ctcgggctcg cgcttcacac gacgccagct gatcggcgcc gcgggcgtcg gcgcgaccgc
    11161 taccgccgcg gccgcggccg gctggaacgg cctagcggtc aacctcgacg gccgccggaa
    11221 catcgtcctg atcgtcgtcg acacgctgcg cgccgaccac ctcggctgct acggcaacga
    11281 ctcgatgcac acgccgaaca tcgacgccct cgcggcgggc gggatgcgct tcagccgcgt
    11341 cttcccagag gcgatgccga cgattcctac ccggcggtcg atcctcaccg gcaggcgggc
    11401 gttcccgttc cgcgactggg agccgtgggc tggcctcggc acgacgcccg gctggcggcc
    11461 gatcgagccc ggggtgccga cgctggtcac cgagctcggc cgccgtggct accggaccgc
    11521 gtgcgtaacc gacaacccgt tcctcggctt cgccagcacc taccggccgt tccggcgcag
    11581 cttcgaccgc ttcgccgcgg tccatggcca gacgccgcgc ggcgacccgc cgccggacat
    11641 cagcatcgaa gaggcgcgtc ggctgatacc gcgcagccgt tgggacgatc tgcgccacat
    11701 cgacgacatg cgccgctatc ttgcggccaa cggcggtcgc ggtggcgacg tcgacgagag
    11761 ctcgacggcg accggccgag tgttcgcctc ggctcggtgg ctcgcccggc ggatgctcac
    11821 caagcgcccg ttcctgctga tggtcgacgc gttcgacccg cacgagccgt gggctgcccc
    11881 atcgtcctac gccgacctct accgcgagcc tggcgacgac gggctcccac cgatgggcga
    11941 cgtcggctat acgccggtcg aggaactgac gccaaccgag ttgcagcgcg tgcgaatcgc
    12001 ctaccgagcg acgctctcga tggtcgacaa ccagatcggc aagctgctcg acacgctcga
    12061 ccggctcggc ctcgcgaaca acaccgtcgt gatgctcgtc tccgaccacg gtttccatct
    12121 cggcgagcgc gactgggtcg gcaagtcggc ctggaggctc acgcccgagc tgacgcgcgt
    12181 gccgctgatc gtgcgtgacc ccgacggccg cggcgcagga gtcgccagcg actggttcgc
    12241 cgacacgcac gacctcgcgc ccacgctgct gtctctgtcc ggtgcgcgac cgtcgaccgc
    12301 gttcgccggc gccgacctct ccccgatcgc cgatggtggc cttcccgacg agccgcgcga
    12361 cttcacctac ggcggctact cgaaccactc ctacgtgcgg gacgacgact gggcgatggt
    12421 caccaacaac gaacgcacag gcgcccagct gtacgacctc cacgccgatc cggacgagcg
    12481 cgtcgacatc gcgagcagcc gcccggaggt ggtcgagcgc ctctacgagc ggatggtcgc
    12541 cgacatcgga gctccaccgc cgtactactc ggcagcggag cttgcgcgaa cgccgcgccg
    12601 ggcgcccgct agctggtagc cgccttaccg aggcgagccg gtaggctggc cgcctggtgt
    12661 gccgggaaga ctggtcggcg accgtctcgt cgacccctga ggaggctcct gtgccgccgt
    12721 tccgtcccat ggcagacccg caaggcgctc gctgatgctg ctcgcgatcg cgatcttcgt
    12781 cgcggcgttg gcactgatcg cgtccgagcg cgtcgaccgg acgaaggtcg cgctgctcgg
    12841 cgcgacgctg atcgtgctga cccagacgat cgaccaggag catgcgatcg aggcggtcga
    12901 ctggaacacg atcggcctgc tcgccgggat gatgctgatg gtcaagctga ccgaggccac
    12961 cggcgtctac acgtgggtcg cgattcgcgc gggacagctg tcgaagggaa ggccgctggc
    13021 ggtcgtcgtg tcgctggccg tgacgaccgg cgtgctgtcg gcgttcctcg acaacgtgac
    13081 gaccgtccta ttgatcgtgc cgatcacgtt cctgctcgcg gacacgctcg acgtcgatcc
    13141 cgtgccgttg atcgtgatcg agatcatcgc gtcgaacatc ggcggcacgg ccacgctgat
    13201 cggcgatccg ccgaacatcc tgatcgccgg tcacaccggc ctgtcgttcg gggcgttcat
    13261 agtgaacctg gcgccgatcg tggtcgtggc gctcgccgtc gtgatcccgg gcctgtacct
    13321 cgtcttccgg tcgaggctgc ggatcgcgcc cgagtcacgt gcgcggatga tggagctcga
    13381 cgccgaccgc tcgatcgagg acgcggccga ggcgaagcgc accgtgccga tcctcgtcgt
    13441 cacgatcctg ctcttcttcg cgcacaaggc gctgcacatc gagcccgcca ccgtcgcgct
    13501 cgccggcgcg agcgtgatgc tgctggtcac gcgtcagtcg gtcgaggagg cgctcagcgg
    13561 aatcgagtgg tcgaccttgt tcttcctgat cggcctgttc gtgatggtcg gcgcgctcga
    13621 ggcgactggc gcgctcgacg aggtcaccga cggcatcgcg tcggtgaccg acggcaaccg
    13681 caccgcggag ctgctcggga tcgtctgggt atcggcgatc ggctcgggaa tcgtcgacaa
    13741 catcccgttc acggcggcga tgatccccgt cgtcgactcg ctcggaggcg caactgacga
    13801 cgcctactgg tgggcgctgg ccctcggtgc gtgcttcggc ggcaacgcga cgatcgtcgc
    13861 cgcggcggcg aacgtcgccg cctccgggat ggcggcgaac gccggccgtc cgatctcctt
    13921 cctctacttc ctcaagtacg gcctcggcgc gaccgcggtc tccttgatcc tggcaaccgc
    13981 gtatgtgatg gtgaggtacg catgacaccg cgagcgatca gcgacgtcgt ccagcgcgag
    14041 gcgccgctcc tgcgcaccga cgagacgatc gagcaggcga cccggacgct gctcgccggc
    14101 gacctgcccg ccctgccggt cgtcgacccc gacgacggct tcgccggcat ctacggcgag
    14161 cgcgagttca tgaccgccgt cttcccgggg tatctcaagc agctcaggta cgccggcttc
    14221 gtcacccagt cgctcgacga cgcgctcgag cgcaacgaga cctgccgcgg cgaggcggtc
    14281 tccaagcaca tgaacaccga gcacgtcgac gtcggcccgg acttcgccga cctccagatc
    14341 gcagagacct tcctccacca ccgcgtgctg gtcgtgcccg tggtcgacca cggaacggtc
    14401 tgcggcgtca tcacgcgcag cgacttcttc cgcgccgtcg ccgggcgctt cctcggctga
    14461 gtcggccgat ggcctgggag ctcgtcgccg ctcattgcga gtcgtcggcc cctggccgct
    14521 cgcgcctgcc ctcgatcagg aacgcgccga ccgtcccggc cagcgacgcg atcaggacca
    14581 ggccgaaggc gaagccggcg agcatgacga tccggccgcc ggtgctcgct gggacgggat
    14641 cgccctggag cgcgagcacg accgcggacg cccacaggat ggcgtcgccg aaggagtcga
    14701 acgccggctc gctgacgtcg cgctcgaaga ggaacgcgag ctgtgccgcg gcgaccgtca
    14761 cgacgacgcc gatggcgccg aggtagccga gccgcgagcc gaacagcgcc cgtgcgctgc
    14821 cgacgctgcg atacgagctc gagaggacgc gggcgccggg aagcgcccga ccgaggcgaa
    14881 cgagccgcag gaagcgcagg agacggagcg tcggcaccag gagcgcaacg gcctggatcc
    14941 agtttcggcg caggaagcgg gcgcgccgcg gcgcgagcca cagcttcacg gcgaactcgg
    15001 cgaggaagac cgcccagatc gtccaaccgg cgatctcgag cgctcgcgcc gcggaggggc
    15061 tgaggtcgac cgcgagctcg tagccgacca ggagggcgaa cagcacgccc agccacgcca
    15121 tcgcgggatc gagccgctcg gtcgcgccgg cggcccagcg ctcgaaggcg tcgtcgtcgc
    15181 cgtggcgcag ctcggcgcag cggcgcctga cgcgcttcgt tcggcggccg ccctctcgcc
    15241 ggtcggcttc gcctgcgtgg gcgtccatgc gtggtaggta cccagcacgc cggtggtcag
    15301 cccggaaaga gcccgatgtg attcggatcc tgcgtgcggc tagcgcgtcg gggcgacgtc
    15361 ctgggcgatg acgctgctcg cggtctgcgt cctcgcggcg acgcacgtcc tcgcggggaa
    15421 gctgcgcttc ctcgagggcg tcccgcggag ccgctggctg tcgttcgccg gcggcgtgtc
    15481 ggtggcgtac gtggtcgtca ggatcctgcc cgagctgagc gccggccagc gcgaggtcga
    15541 ccatccggcg aacgcgctgc ttccgttcct cgagggccac gtctacctgc tcacgctcgc
    15601 cgggctcgtc gccttctacg gggtcgagcg ctcgtcgtcg agttcgcgcg cgcgacggcg
    15661 cgaggcctcg gggatcgacg cgaccgaccg ctcggcgttc gtcgtcagca tcggcggctt
    15721 cgccgtctac aacgtcatcg tgggctacgt cttggcggac ttcgacgcga cgcgcgagca
    15781 ctcgctggcc gccctcgcgg ccgcgctcgc cgtccacttc ctcgtcaacg actacggcct
    15841 gcgcgagcac cacaaggacg cctaccggga tgtcggccgg tgggtgctgg ctgccgcggt
    15901 cctggtcggc tgggcgatcg ggcggtcggc ggagctggac gaggccggcg tcgcgctgct
    15961 cgtcgcgttc ctcgccggag gcatcatcct gaacaccttc aaggaggagc tgccgcgcga
    16021 ccgcgagagc cgcttcctcc cgttcgtcgc cggcgcggcc ggctatgccg cgctcctgca
    16081 gctcgtctga tcgggacgcc ggtgtgaagc aagcgggatt gtgggtaccg catgctgaac
    16141 cgacgaaagg aacgcccgtt gctggagcgc gtgcgcaaca ccttctgggc gctgccggcg
    16201 ctcgggatag cggtagcgat cggcgccgcg ctggcgctgc ccgtcctgga cgacacgatc
    16261 accctgcccg acgccgtgtc gttcagcggc agcgccgaca cagcccgggc gaccctgcag
    16321 atgatcgcca cggtcgcggt gtctgtcgcc gggatcagct tctcggtgat cgtcgtcgcg
    16381 ctggtgctgg cgtcacagca gctcagcccg cgcgtcatgc gcagcttcca gcgccatccg
    16441 ctcaaccagg cggtcctcgg cctcttcctc gcgacggccg cgttcagcct gttcgtgctc
    16501 ggctcgatcg acgacagcag cgacgaggtc ccggaggtgg cggtgactgt tgcgatggtg
    16561 cttgccgccc tgtcgctcgc gacgtttgtg gtcttcctgc accacatggt gcggtcgctg
    16621 aacgcgtcgg ccgtcatccg gcggattgcg gcggagggtc acgaggcggt gcacacgccg
    16681 tatccggagt acgtgggaga ggcgccgggt gacgccgagg cggccgagcg cgacgcgcgc
    16741 gcgatcgtcg agcggtcggt ctcgcgtgag gtccgcgcgc cgcgggccgg ctacctggca
    16801 tcggtgaagg cggacgacgt cctggcgacg gccgacgact gcgacggctt cgtcgagcag
    16861 cggcaggcaa tcggcgactt caccgtgacc ggtgggctgc tggcggtcgt ctggtgctcg
    16921 cgggagagcc tcgacgagct ggccgagcgg gtcgagactg cgttcgtgct caacgaggag
    16981 cggctcgtcg acggtgacgt cgcgtttccg ctgcgccagc tcgccgatgt tgcgctcaag
    17041 gggctgtccc ccgggatcaa cgacccgacg acggccgaga acgcgatgga ttcggtgacg
    17101 gacagcttgg tgcggctggc gcggcaacca gagccggcgc tgcttcgact ggcgccaagc
    17161 gggacgccgc gcctgcgcgc gacgacgccg agcctggacg cgctgatcaa gctcggcttc
    17221 gaccaggtcc ggcgcgacgg tgccgcgcag ccgtcgttcg cggtgaggct gttggagctg
    17281 ctggcggcgc tgcgcgacgt cggcggcgcg cgtgccgcgg gctccgagga actggaccgc
    17341 caggctcgcg ccgtgcgaga ccatgcgatc gcgctcgccg acattcccgc ggacgtcgag
    17401 ctgctgcgcc gctcgtacga ccggctgcat ggctctgccg cctttggcga cggccgcggg
    17461 tcgttgcctc gcgacgtcgt ggcgcgttag acgccggcgt caatccagtg cgagctcgtc
    17521 ctggagcggc ggataggtga ggtaggtgcc gtcgacgacg gcgaacggcg tcagctcgtg
    17581 gcgttgcgtg cggccgtcgg gcatgaggtg gacgacggcg tgcccgcgca cgagcagcgc
    17641 gtcggcgagc agacggcggt ggcagcgcca ccagggcgcc tcggcgcaca tcgcggcgac
    17701 gctgcgttcg tccgcgagcg cgagcagccg gtcgaggccc gcctcgaact cggcgctctc
    17761 catgtggtcg gcgtacccct ggaactgacg cacctcccag ccgccgttgg gggagtccgg
    17821 gaccggctta cgccgcccgc cgagctcggg aaggtgctcg tagccgatcc ccgcgtcggc
    17881 gagccactcg cgcatcgcct cggcggcaaa ccacggcatc ctgcgcgagc cgggaaagcg
    17941 ccgcacgtcg gcgatcgcct ccacgctcgc gccgcgcagg agctccacca accggtcggg
    18001 ctcgtgggtc gagtggccga ctgtggtcag gattggcact gtcgtggcca tggcgcgagc
    18061 gtacgcatac ctgcttcagc gcttcagagc ggctcccggt caagcccacg cagtcctaga
    18121 ctccgatgcc gtgccgtcgc cagagcagcc atgaagcgga gggtgctggt cctcggcgct
    18181 ggcttcggcg ggctcgagct ggcgacgcgg ctgtccgaga cggtcgcgga cgccgtcgac
    18241 gtgacgctgc tcgaccgcaa cgactcgttc tacttcgggt tctccaagct cgacgtgatg
    18301 ctgggccgcg agggggccga cgcggtccgg ctgccgtatg gcgacatcgc gaaggacggg
    18361 gtcgagttcc gtcaggagac ggtgaccgcc atcgacccgg aggcccggcg cgtggcgacg
    18421 gacgtgggca cctacgaggc cgacttcctc gtcgttgcga tgggcgctga ctacgacatg
    18481 gcggccaccc cgggcttcga ggagggcggt cacgagtact acaccctcac gggcgccgag
    18541 cgcctgcgcg acgcgctggc cgacttcgac ggcgggcgcg tcctcgtgtc ggtcctcggg
    18601 cagccgttca agtgcccgcc cgcgccgttc gagggaacgt tcctgctgga cgagcacttc
    18661 tccgaacagg ggatccgcga cgcggtggag atggcgatcg cgtttccgat gcagcgcccg
    18721 gtgccggtga ccggcgaggt gtcgcagatg ttccgcgacg gcctggcgac gcgagggatc
    18781 gaggagcttc cccagcacct cgtcaccagt ctcgacccag ccgcgggagt ggcacggctg
    18841 gcgaccggca agacgctccc ctatgacctg ttcgtcggca tccccgtaca ccgcgcgccg
    18901 gacccgctcc ccgcctcggg gctggcggta gatggctggg tccccgtcga ccaggccaac
    18961 ctccggacgc gcttcccaca ggtctacgcc ctcggcgacg tctgcaccgg ccctcggacc
    19021 gttccgaagg ccgggatctt cgccgagtcc gccgcgctgg tcgtcgccga cgacatcgcg
    19081 gcgaaggtct ccggcgcgga gccgccgccg ccctacgagg cgtcgggggt ctgctacgcc
    19141 gagttcggcg acggactcgt cggcaaggtc gagatcaact tcctacgggg cgatgcgccg
    19201 gcggcgcagc gtcacgatcc ctcgcgcgag caggccgccg agaaggccga cttcggcgcg
    19261 acgcgccgtg cgcgctggtt cggggcctga gcgcacctcg tcggcgagac ttccccaaga
    19321 tcatcccagc ggaagccgcg tgggcgatcc agggcagcgc tcgagcagct gctgtcttgc
    19381 gtcccgactc gaccggcaag tgttcctctt tgaccaaggc catgaactca ctatggcagt
    19441 acacatccat ccgtcgatcc ggctgcctag tctcgaccga tggtccacga ttcatcgagc
    19501 caaccgtcga gcgcgaccct ctccgcctcg caactgattc cactactgcg cgcgattgca
    19561 gatccggagg gcgacggcac gatctcccct caggacgctg cgtcaataga acgggtcgag
    19621 acgaacctga cccgcttcct cggcctcggc tagcccttga tcgcctcagc gaatcaagac
    19681 gacttcgaca cccacctcga cggtccccca ggcccgatca gccgtatgca cgggaagacc
    19741 aagtcgtcgc gctagagaca ggcacgccct gtcggcgagc gacaggcctg ccgtgcgcgt
    19801 cgatgcccac atgcttgccg caatctcagc gtcgtcgcca ctgaagtcct cgatggagac
    19861 cccaagcgcc tcgaggctgt ctcgcctgcc tgtgagatcc aacccgagcg cgttgtagcg
    19921 ctggagcacc tcggaccagt tgacggctga gatgacagcg gcgccaagca acccctcgac
    19981 agcgtcggac cccgactcct cccagagcag ggcgatcacc gcggaggcat cgagaaccgc
    20041 tccgggcccg tcagccatgc tcggcagcct cgcggcgcgc ctcggcgcgc cgctcagcga
    20101 tcaactcgtt gaccatgctc gtcccggcgg gagtcgttcc gcgcaactcg ccgcgcagcc
    20161 gctcgaggat ctgctcccgc cgctcgatca ctagccgatc ttcctccaca tgtgccatca
    20221 gcgtctcccc tggctgaatc tccaacgtcc gcctcatctc cgcagggatt accacccggc
    20281 cctgaggtcc gatgcgcaaa gggactgctc tagctcgacg tgccatatcg atcgaagtgt
    20341 ggcagatgcg ccgaggcgtg gcaaccgaat tgctcgacgc cgggatctca acaccggacc
    20401 ggtagggtcc agcacctgcc catggtcccc ctcccggtag cgccgccgcc cgcgatcttc
    20461 ccggcggtaa ggatcccact gcttgtttcg tgcccttgct gatccggagg gcgagggcaa
    20521 gggagcaatc acgctgacga cgccgcagcg atcgagcaga tcgaaacgaa cctcgccgct
    20581 accgcaacgg cagcgagagc cgacgcccgg attcgaaccg gggacccctt cattacgaga
    20641 gcgttgagaa ggcgtcgagc tgggtcgatt tggcttgggt aaggccagct cggtcaggct
    20701 cgctcgggtc agatttgccg agttggggac atggttcggg acacgctgtc accagcacca
    20761 ggcgactcta ggctccgacc tgacgaaccc aatcgcgaac catcatcccg actcgctcta
    20821 catcctccac gccgaggaag ccactatgca tgatgacgtt tcgggatcgc tcaaccgcat
    20881 caaagatgct ctttgcccac tccggcgacg gccaaaatgg cgcaaaggcc tcctcgtttg
    20941 cccgcatcac attcagcaag tccccgaagt cgatgaagct aacgggatcg tcgcctcgct
    21001 gagtgtgaaa ccggtgcttg gcctcgtcct cctgtcggcg cttagccttg ccctggatgt
    21061 tgttggacac gcaggtgtcc caccactcgg ctccctcctg ctcaactagg acccgccgca
    21121 ccagatcccg cacggtgttc tcgaaggccg cgatcgccgt gtataccgtg gccatcctcc
    21181 gagcctgggc gacaaaatga tcgtccagta ggtccaatga gaccttctcg gcaacctcct
    21241 cgtccagaaa cgggagagcc gccttcgggc gccctccgtg gtcgagcgcc tgctctgcca
    21301 tgaggccacg gaaggcgaag tcgtagaggc ttggctgacc ggtcaagaga gtaggtgagt
    21361 cttcagcgcc cggaacaagg cctcgtacac ggccggatca cgcgagtctg ggaggtggag
    21421 ctccacgttg tagacaaacc cgccgagctt gatcgcgcca gcactcgacc ctccaagatc
    21481 gataggcggg acgtcacgtt ggtcgtctgg cgcagaaatc tcagcctcct cagtcgcctc
    21541 cgtttggttt ggcctcgggg catcgaaatc tgcttgctcg gcgagcgcaa ggaaggtcat
    21601 ggccatcttg tctacgacgt tgtcgctgac ctgaccttga gtcagcgtct tgatcttgcc
    21661 tcgcagctcc tgccgctcca tgttctgggc gtcccgccgg acctggaaaa gatccgcata
    21721 ggcctcctct agtgcctctg cgagaactcg gcgtgattgc gtctgatcca tgaactcgaa
    21781 gtaacgagcc gttggacttc cgtcctcaga aagcaagccg atcgccttga acacgttgat
    21841 atacagccga tcgtttgtgc tcttgaaatc caactcctcc aggaaccgct gagtgaactt
    21901 cggcggtgcc ttggcattgc ggagtgcgtt gaacagcggc tcaacattcc gggcggtcat
    21961 taggtaggca gacggtaagg ccataggaga gcgatcctaa gctcgcgcac ggacacctaa
    22021 gaacacggac gtacgcaaat gtgcccggcg agcgcctcgg ctcgggctac agcggagccg
    22081 cactcagcgc ggattgggcg gcgcgggtac acctagttgg acgcagatcg atcgaaccaa
    22141 gctcgggccg atctccttgt gtcgcggaac ggccgaaacc tggtcctgca ggccgcgcca
    22201 cttcgcatgc ttggtcccgc cgatctctcg acagccgtgc gagcgtagat ggcgttcgag
    22261 atcgcgccgc ttcacgcggc ggcggtgaaa cgcagatgct cgcgatcggc ttgcggggct
    22321 ccgcccgcta gctcctcgtc aggcgtgagg accgtgctga gagcgtcgag ggcgttctcg
    22381 cgggcctcgt cgcgcgtccg gccctggctc atcgcgccgg gaacctcgag gacgcgggcg
    22441 acgatccagc cgtcctcgtc gggcggctcg aaggcaatcg tgaagtcgat tgatgcggcc
    22501 atcgggaaca cggtaccggc tggctcgtgg ggcgtccaga aggctgccgg aaccgtcgca
    22561 ggagtggccg cgcaccccta ggtgcaggtg tgtggtccgc tgggcggtgg tcggagaaga
    22621 gggcggttgg aggcggcgag gtcgtggggc ggcgcgtatc taggcgagtc agcacgtaga
    22681 gggggcggtc gcgtcggggg cgtgggtcgg atcgctggcg ggacgaggaa ggcgagcagt
    22741 agtcgagtga ggagttggcg gtgggtcgcc cgccaccccg gcggcgctcg ctcaacggag
    22801 cacggcgatg ccagagatcg gggcgcattg cgtctcctcc ttcaagccca tgagtccgcc
    22861 cgatatctcc cccaccgatc ccgccgctac agcttcgttc ctgtccgagc cgctgcttga
    22921 ttccgacgca gcggcggcgt tgctgtcggt gcggcggagc tggatctacg aggccgtgcg
    22981 cgacggccga ctcccccacg tcaaagtcgg ccgccacatc cggttcctgc gcgctgacct
    23041 ggagagctgg gtgttcgccc agcggagagg ccggctgttc taggcgagcg ccaactcggt
    23101 cgccttcgcc gctgcgcggg tggcgaacaa gaagccgtcg agccggtgga cgagaccgat
    23161 cgcggcgagg cgattgatgg agtcggtcac gtgtatgggg ttgccgatct cgctggccat
    23221 ctcctcgacc gaccagatcg ccgcagagtc cgtcagcaac aacccgagga ccgcctggtc
    23281 tgtctgcgcc tcctcgtcgg ccactgtctg gatacgctga tcctgcatgg gagctagtac
    23341 ctcctgtgcc aggccccggg acgttcacgc gtcgccgggg ccactttgta gttgcttcga
    23401 tactaacggt ggactactag atgtcaacta catggactgc acggcgagca gttgacaggc
    23461 tgtagttgac gatgggacgc ccgatcaacc cccagcccgc cctcggtgag gcgatccgcc
    23521 agctacgtac caagcgcggc atcagccaag aggatctagc ccacctcgcc ggcgtcacca
    23581 ccggcaccct ctcggtcatc gagcgcggac gctccaaccc cgcgtgggga accgtcaagg
    23641 cgatcgccaa ggcactcgac gtatcgatgc ccgagctggc gaagctcgct gagaagcacg
    23701 aacgctgacg cgcagctaag cgtcgaaggc gctcgcgacc agcgcggcgt cgtccttgcg
    23761 cggggcgtag tggaggtagc gcatggtggt cttcacgtcg gcgtggccca tccactcctg
    23821 gacgcgacgg atgtcggcct tggcgatcac gcgggtgccg aaggtgtggc ggaggtcgtg
    23881 gaagcggagg cgacggaggc cggcgcgatc gagggcgtct tggtagcggc ggcgtagggc
    23941 gcgaccgtcg aggaagctcc cggtgcggcc gacgaagacg aaatcgtcct cagccgtcca
    24001 cagcgggcgc tgacggagat tggccagcgt ctcggcgacg ttgggtgcca tcgggaccga
    24061 gcggaccttg ccggacttgg gagtggcgag cgcgccgtcg gagtagctgg cacgtacgcg
    24121 gatggtggaa ccctcaatgt cgatgtctcg ccagcgaagt gcgatcagct cgccgcgacg
    24181 aagtccgctg aaggcggcgg tgaggtagat cgcggcgtcc tgctcagatg ctgccgcgtc
    24241 gaccagcgcc atgacctcct ctggcgagaa gacctcgatg tcgccgctgg ccgttggcgc
    24301 tggcggcttc tccacgtcgg tgacggggtt gaacgggagg ccgtagacct tgcgcgctcg
    24361 cttcatgatc ccgcccatca gcacgtaggc cttggtacgg gtggctgggg cgacctcgag
    24421 cccggctatc caggactcga ccatcggagt cgtgatcgac tcgacgggca gcttgccgaa
    24481 ggctggaagt agctgagcgc ggacgagggc gcggtagccg gcgaccgtgg agggcttgcg
    24541 agcgcggtcc tcctcgatgt agcgcaggta ctcgtcggct gcctgctgaa aggtcgcgcc
    24601 agttcggacc atgccggcgt gctggccgcg ccggatctcg tcgatgatct ggtctagctg
    24661 tgcttgcgcg gtctgcttgg tgaagtagcc gtctgagggt cggccccgct tcgtccacgc
    24721 cggtccgagc ttcttctgca cctggcgacc atcgggcagc cggtacttgg cgtaccagac
    24781 cggcccgcgc tggcgctcga ctcggaatac gtggccgctg accggccggc tgagttggct
    24841 ctgcattggg gacctcccag gcagcccgat tggctttcta gagcccaaag ctagcagcgt
    24901 ttcgggacat attcgggaca cgcggcccga cgagcggaaa gccgatcagc ggaaacgtgc
    24961 tgatttccag ggctttccga gagccgacgc ccggattcga accggggacc ccttcattac
    25021 gagtgaagtg ctctaccagc tgagctacgt cggcggggga tgcgagtcta gctcgcggat
    25081 tcggtcgggg tggggctagg cggcggcgtg gacgagcggc cagcgggcgg aggtgacgcg
    25141 tggtggggag agctcgcctg tgacctcgac gagcgagggg ccgtcggtgg cgagggcggc
    25201 ggtaagggcg ctctggaggt cgtcgggggc ggctaggtcg gcgttccagg cggggacgcc
    25261 gaaggagcgg gcgagggcgg cgaagtcggg ggcctggagg tccacggcga aggtgcggcc
    25321 gaagtggcgc tcctcgtcat agcggagcat cccgtagcag gcgtcgttgt ggacgacgac
    25381 ggtcacgtcg aggcgctcct gggcgagggt ggcgagctcg ccgattgcga agaggacgcc
    25441 accgtcgccg cagatcgcga gcgtgcggcc ggagtgcgcg gtcgcggcgc cgatcgaagc
    25501 gggcagggcg aagccgagcg tgccccagcc gagcgggtag aggatgcgcc tgccgggccg
    25561 cagcgggagg tagccgccgg tccagtaggc gctgacggcc atgtcgcaga cgagggcggc
    25621 gtcgcggtcg ccaagcgcgg catcgagcgc ggagaggtag gcgagcccgc cggaggcgcc
    25681 ggggtcgtcc gcgagcgcct ggcgggcggc gacgtcggcg gcgacgacgg cctcggggcc
    25741 ccagccgccg gggcgctcgg ggacgaggtc gagcagcgcg ggcactacct cggcggcgtc
    25801 ggcgacgacg gcctgcgcgc tggcgtagcg gtcgtcgccg gcggcgcgct ggggatcgac
    25861 gtcgacgcgc aggatcggtt cgagcgggag gcggaactcc tgggtcatca tgccgtcgag
    25921 gtcgctgccg agcacgagca gcgtgtcgca ctcgccgagc agctcgccga tcggcggctc
    25981 gtgcggcggg taggcgggga cgagcggatg cggagccgcg ttccagcggc gcttcgcgct
    26041 gtgggtgagc acgacgggcg cgtccaggcg ctcggcgagg gcgacgatcg cggcgccgca
    26101 ggccgcggcg ccggagccga cccagatcgc cgggcgctcg gcggcggcga gccggtcggc
    26161 cagggcgcgg agctcggacg aggccggctc tggcgggtcg gcacgatcga gtccgttcgc
    26221 cgacaggccg ctcgcgcgcc tcgacccgtc tcccgaaacg cccgccggtc ccgcctcgtc
    26281 atcgagcagg tcagtcggca gctcgacata ggccggcgcc cgcggcgcgg cctcggcggc
    26341 cctcagcgcg tcgcggatat ccctcgccgc gccggcgccg ctgtacgcat gcagcgcaac
    26401 cttcgacacc ggcacgaaca ggtcgcgctg gcgcgggtgc tcgtgcagga cgccgcgcga
    26461 ccgctctggg tcgagatggc gccgggccac ggtcgtcgtg atgtgcagca gcggcgagcg
    26521 cgacgcctgc gcctccccca tcgcggtgat cgcgttcgcg gcgcccgggc ccgacgtcgt
    26581 cacgcagacg cccggccgcc cggtcaggcg cccgaccgcg tcggctgcgt aggcggcggc
    26641 cgcctcgtgg cggacgacga tcgtgcggat cggcgacgcg tcgagcgcgg cgaacagcgc
    26701 caggttgtgg acgcccggca ggccgaagac gacctccacc ccggcgtcaa cgagctcgtc
    26761 gacgaccgcc tcagcgacgt tgcggctcat cgcggcgccg gcatgtcgag gtagacggtc
    26821 ttgagatcgg tgtagaagtc gcgcgcggcc ttgccctgct cgcgcgagtg cgagctcgag
    26881 tccttcatgc cgccgaacgg gacctgcggc tcgctgccgg cggtctcgga gttgacgtgc
    26941 acgacgccgg cctcgacctc gcgcgcgaac tcgaacgctc ggccgacgtc gcgcgtgaag
    27001 atcgatgccg agaggccgta gtcgacgtcg ttggcgatcc gcacggcgtc ctcgatcgag
    27061 ctcgccggga tcacgccgag gaccggcccg aagacctcct cgcgcgcgat ccgcgagtcc
    27121 tggccgacgc cggtgtagac ggtcggtgcg acgaagtagc cgccgtcgag gtcgatcggc
    27181 tcgccgcccg cggcgagctc gtggccctcc tcgcgcgcga cgtcgaggta ggagagcacg
    27241 gtctcgtact ggccctgcga cgccagcggg ccgacgtcgg tgtccggcgc cgacgggtcg
    27301 ccgacgcgca gcgccgacac ggcgggcagc agcgcctcgg tgaaccggcc cgggtcgccg
    27361 acgacgatcg cgcggctggt agctgtgcac ttctggccgg tcgagagcat cgcgccgcgg
    27421 accgtctcgc gcaccgcgag gtcgacgtcg gcgtcgtcca tgacgatcac cgggttcttg
    27481 ccgccgagct cgagctggac cttgacgccg cgctcgacga cgtcgcgctg gatcgcccgg
    27541 ccgaccgcgg tcgacccggt gaacgagatc ccgtcgagct gcggcgccga cacgagcgcc
    27601 tcgccgaccg gcgagcgtcc gcccgtgacg aggttcagca cgccggccgg cagcccggcc
    27661 tcgtgcagca gctcgacgag gttgaccgcg cagcgcgggg tcagctgcga cggcttccag
    27721 acgaccgcgt tgccgaacgc cagcgccggg gcgatcttcc agaccgggat cgcgagcggg
    27781 aagttccacg gcgtgatcac gccgacgacg ccgagcggga cgcgctcggt gtagaggtgg
    27841 cggtcggcga cgccgctcgg gtagacctcg ccgagcggct gcgcggtctc gccggcgaag
    27901 tagcgcagca gtgcgaccga gcgcagcgcc tcggcgtgcg cct
//
LOCUS       DATABV010000013         5989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_85223, whole genome shotgun sequence.
ACCESSION   DATABV010000013 DATABV010000000
VERSION     DATABV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85223"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VHF88_00505"
     CDS             <1..1198
                     /locus_tag="VHF88_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2160286.1"
                     /translation="EDARAMPAPATRAAAVGARLGLAPASGIPAPPPRRIASIEPELP
                     QAERRSRWQKVRRAAAIGGAAAGVGLAALALERIGIDAIGRALLAATPVWVLAAFALM
                     CASMLVRAEAWHAILSAALPGVRVRRRDVTRATMIGVLMSATLPARLGEPSRALIMSR
                     RIGRVRDRFPVVLGSIVSQTLLNILALIVLGSVMFATVGVFRGGEDALVIATIAPLVL
                     LALVVGAPALLRRGKPSRFARVQQALAVARSAMVQVRRGLEVFRRPRLGAWSTAMQLL
                     AWGVQWLACYVLLVALGLDGQAGIGAAAAVLFAVNVTAALPVTPSNLGVFQAACVAVL
                     SAYGVGKTDALAYGIILQAVEIATAFAMGAPALLREGMTWRDLRMRALHATPVELRAV
                     RGGVEA"
     gene            1348..3627
                     /locus_tag="VHF88_00510"
     CDS             1348..3627
                     /locus_tag="VHF88_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2160287.1"
                     /translation="MAPAATLAEPLRPAASAPLVPRTGERVAQPAFVPGEAIVRFESG
                     TTAAERLSARRAADAELERSLAVPRAQLLEVDGDVAAAVERLERQPDVAFAQPNYRYR
                     ALAAPVPNDTFFGDLWGLGSTALPAPGVDALEAWESNRGGGQVVAVVDTGVAIDHPDL
                     QGNLWTGPGGIHGHDFVDDDSDPDDYEFHGTHVAGTVAAVDGNGLGIAGVAPDAQIMA
                     IRVLDGNGSGSSADIGAGIAFAAQNGADVINLSLGGDADSDPLMSSGIAIADSLDAVV
                     VAAAGNESNDNDANGTVPCNLPHPNVICVAAVNEAGELADFSNYGATTVDVGAPGTRI
                     LSAETDYAAVHSDGFASFADLTTGTANGGVAWGTVATPNTDGNPSAADSPSGNYGQAV
                     DHGFYAASVLAKATPLSLAGRYGCRMAFHLRYELEDGYDLFFAGTGTPDPNVDPFVTG
                     SSGGSFFDEGFSISHADGSASVTPRFTLLSDYLEERDGVYLDQWRVLCRDSSYSNAAP
                     PTGNYVRFQGTSMAAPHVAGVAALVRAAVPGLADTAVVQAIEEGGAPLDSLSGTTVSG
                     RTADADGAIAVGLGEQPPSNDTGAPPDPTQPVPTQPDPTLPSPTQPDPVQPDPSPLPG
                     PAPPPPAPRPVPHSPLSPPLASSLSLAGAKAALRVSRRGVFRYTFRATPSLAGRIAFR
                     TRRKVRIARRTHLSLAAKTFSAPATGRVAVKVRLSKRQLRILRRNGRFLLRVTATARG
                     NAGESLRASRGLTLRPPIR"
     gene            3677..4666
                     /locus_tag="VHF88_00515"
     CDS             3677..4666
                     /locus_tag="VHF88_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2160288.1"
                     /translation="MTSQSSLAIEASGLVKDFRTTRAVDGVDLAVRRGSVYGILGPNG
                     AGKTTTIRMLATLLRPDAGEARVMGHDVVTEADAVRSLVSLTGQLASVDEDLTGHDNL
                     LLIGRLLGFKRQQAKARAADLLAAFELGDAADRQVKNYSGGMRRRLDIAASIVVTPEL
                     IFLDEPTTGLDPRSRSQVWEIVRALVADGTTVLLCTQYLDEADQLADGIAVIDRGKVI
                     AEGTPAQLKASVGSGALHIRLLDPERRHDAERLVAHAVGAAVQLEPDPAALSVPVADA
                     ARAAEAVAELARMGIGIADFSLGQPSLDEVFMALTGHPAEDSGATDPTAKEAA"
     gene            4663..5517
                     /locus_tag="VHF88_00520"
     CDS             4663..5517
                     /locus_tag="VHF88_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2160289.1"
                     /translation="MSSTDVTSTGAAEAMPDAAALRAVLAASERPPRPGRLAASLTFG
                     WRGMLKIKHVPEQLLDVTITPVMFVLMFTYLFGGAIAGSTGDYLQFILPGILVMSVLF
                     TTVYSGVTLNTDMTKGVVDRFRSLPVWRPAPLVGALLGDAVRYLVAGTVIIVLGLILG
                     YRPEAGPGGVVAALALVVVFAFGLSWAFTTLGLVMRAPNAVMNTGFMLLFPLTFVSNA
                     FVEEETLPGWLQTVVDVNPISHLITAARGLMDGNADGGEVLLVLAVAAGLTAVFAPLT
                     TRLYRRAA"
     gene            5580..>5989
                     /locus_tag="VHF88_00525"
     CDS             5580..>5989
                     /locus_tag="VHF88_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX2160290.1"
                     /translation="MADRDYLQAVRERVVVFDGGMGATLEQFDLTQADYGGLQGKCHE
                     ALVLNRPDVIEGVHRSMVEAGAEVVETDTFQASRLKLEEWGLAEHTVEINRRAAEIAR
                     KAVGEDRFVAGSIGPTGFLPASDDPTLGDIRFRDL"
BASE COUNT          772 a         2248 c         2089 g          880 t
ORIGIN      
        1 cgaggatgcc cgtgcgatgc ccgctccggc gacgcgcgcc gccgccgtcg gggcgcggtt
       61 ggggctggcg cccgcctcag gcatcccggc gccgcccccg cggcggatcg cgtcgatcga
      121 gccggagctc ccgcaggcgg agcgtcgcag ccgctggcag aaggtccgcc gcgcggcagc
      181 gatcggcggc gccgcggccg gcgtgggcct cgcggcgctg gcgctcgagc ggatcgggat
      241 cgacgcgatc ggccgcgcgc tgctcgcggc gacgcccgtc tgggtgctcg ccgcgttcgc
      301 cctgatgtgc gcgtcgatgc tggtgcgcgc ggaggcgtgg cacgcgatcc tctccgcggc
      361 gctgccgggc gtccgcgtgc gccgtcgcga cgtcacgcgc gccacgatga tcggggtgct
      421 gatgtccgcg accctgcccg cccgcctcgg cgagccctca cgcgccttga tcatgtcgcg
      481 gcggatcggc cgcgtccgcg accgcttccc cgtcgtgctc ggctcgatcg tctcccagac
      541 gctcctcaac attctcgcgt tgatcgtgct cggttcggtg atgttcgcga ccgtcggcgt
      601 cttccgcggc ggtgaggacg cgctcgtgat cgcgacgatc gcgccgctgg tcctgctcgc
      661 cctcgtcgtc ggcgcgccgg ccctgctgcg ccgcggcaag ccctcgcgct tcgcgcgcgt
      721 gcagcaggcg ctggccgtcg cccgctcggc gatggtgcag gtccgccgcg gcctcgaggt
      781 cttccgccgg ccgcgcctcg gcgcctggtc gacggcgatg cagctgctcg cgtggggcgt
      841 gcagtggctc gcctgctacg tcctgctggt cgccctcggc ctcgacggtc aggccggcat
      901 cggcgccgcg gcggcggtcc tgttcgccgt caacgtcacg gcggcgctgc cggtcacgcc
      961 gtcgaacctc ggcgtcttcc aggccgcctg cgtggccgtc ctatccgcct acggcgtcgg
     1021 caagaccgat gcgcttgcct acgggatcat cctgcaagcc gtcgagatcg cgaccgcctt
     1081 cgcgatgggt gccccggccc tgttgcgcga gggcatgacg tggcgcgacc tccgcatgcg
     1141 cgcgctgcac gcgactccgg tcgagctgcg cgccgtgcgc ggcggcgtcg aggcctgagg
     1201 cctcggactt cagaggttcg gctcctgagc cgatgcattc ggtggatcgt tcgcagcggc
     1261 tgctgtcagc gggcatgatc cgacaggggg gatgacctga tgcgatcgcc tctcaaaccg
     1321 atactcctct tccttgcgct gtggctggtg gcccccgcgg cgacgctggc cgagccgctg
     1381 cgccctgcgg cctcggctcc gctcgtgccc cggaccggcg agcgggtcgc gcagcccgcc
     1441 ttcgtgcccg gggaggcgat cgttcgcttc gagagcggga cgaccgcagc tgagcgcctg
     1501 agcgcccggc gggcggccga cgccgagctc gagcgctcgc tcgcggtccc acgcgcccag
     1561 ctgctcgagg tcgatggcga cgtggccgcc gcggtcgagc ggctcgagcg tcagccggac
     1621 gtcgccttcg cccagccgaa ctaccgctac cgagcgctcg cggccccggt gccgaacgac
     1681 accttcttcg gcgacctgtg gggccttggc agcacggccc tgccggcgcc gggcgtcgat
     1741 gcgctcgagg cctgggagtc caaccgcggc ggtggacagg tcgttgcggt cgtcgacacc
     1801 ggcgtcgcga tcgaccatcc cgacctgcag ggaaacctct ggacgggccc gggcgggata
     1861 cacggccacg acttcgtcga cgacgacagc gatcccgacg actacgagtt ccacggaacc
     1921 cacgtggccg gcaccgtggc ggccgtcgac ggcaacggac tcgggatcgc cggcgtcgcg
     1981 ccggacgcgc agatcatggc catccgcgtg ctcgacggga acggcagcgg ctcgagcgcc
     2041 gacatcggcg ccggcatcgc gttcgccgcc cagaacggcg ccgacgtgat caacctcagc
     2101 ctcggcgggg acgccgacag cgacccgctg atgtcgagcg gcatcgcgat cgccgactcg
     2161 ctcgacgcgg tggtcgtcgc agccgccggc aacgagtcaa acgacaacga cgccaatggg
     2221 accgtgccct gcaaccttcc ccatcccaac gtcatctgcg tcgctgctgt gaacgaggcc
     2281 ggcgagctcg ccgacttctc caactacggc gcgacgaccg tcgacgtcgg cgcgcccggc
     2341 acgcggatcc tgagcgccga gaccgactac gccgcggtcc actccgacgg cttcgcctcg
     2401 ttcgcagacc tgacgaccgg caccgccaac ggtggtgtcg cctgggggac cgtcgcgact
     2461 ccgaacaccg acggcaaccc ttccgccgcc gacagcccga gcggcaacta cgggcaggcg
     2521 gtcgaccacg gcttctacgc ggcctcggtc ctcgccaagg cgaccccgct cagcctcgcc
     2581 ggacgatacg gctgccgcat ggccttccac ctccgctacg agctcgagga cggctacgac
     2641 ctcttcttcg ccgggaccgg gactcccgac ccgaacgtcg acccgttcgt caccggctcg
     2701 tccggcggct ctttcttcga cgagggcttc tcgatctccc acgccgacgg ctcggcttca
     2761 gtgacgccgc gcttcacgct gctctccgac tacctggagg agcgcgacgg cgtctacctc
     2821 gaccagtggc gcgtgctatg ccgggacagc agctactcga acgccgcgcc cccgaccggc
     2881 aactacgttc gcttccaggg gacctcgatg gccgcgccac acgtcgccgg tgtcgcggcg
     2941 ctcgtccgcg cggccgtgcc aggcctcgcc gacaccgcgg tcgtgcaggc gatcgaggag
     3001 ggaggcgctc cgctcgactc gctgtccggc acgacggtct ccggtcgtac cgccgacgcg
     3061 gatggtgcga tcgctgtcgg cctcggcgag cagccaccct ccaacgacac cggggcaccg
     3121 cccgacccga cgcagcctgt ccccacccag cccgatccga cgctgccgag tcccactcag
     3181 ccggatccgg tgcagcctga tccatcgccg ctcccaggtc cggcgccgcc cccgcccgct
     3241 cctcgccccg tgccgcactc gccgctgtcg ccgccgctgg cgtcgagcct gagcctcgcg
     3301 ggtgcgaaag cggcgttgcg cgtctccagg cgaggcgtct tccgctacac gttcagggcc
     3361 acgccgtcgc tggccggccg gatcgccttc aggacgcgca ggaaggtgag aattgcgcgc
     3421 cggacgcacc tgtcgctggc tgcgaagacg ttcagcgcgc cggcgaccgg gcgcgtggcg
     3481 gtcaaggtga ggctctcgaa gaggcagctg cggatcctgc gacgcaacgg gcgcttcctc
     3541 ctgcgcgtga cggcaaccgc gcgcggcaac gccggcgagt cgctgcgggc gagccgcggc
     3601 cttacgctgc ggccgccgat acgctgaccc ggccgcgttc gcctaacgtg ccgggggagc
     3661 gatcgaagga gcggtcttga cgtcccaatc gagccttgcg atcgaggcca gcggcctcgt
     3721 gaaggacttc cgcacgacgc gcgctgtcga cggagtcgat ctcgcggtgc gccgcggcag
     3781 cgtctacggc atcctcgggc cgaacggggc cggcaagacg acgacgatcc ggatgctcgc
     3841 gacgctgctg cggccggacg ccggtgaggc gcgcgtcatg ggccacgacg tcgtgaccga
     3901 ggccgacgcc gtgcgctcgc tggtcagcct caccgggcag ctcgcctcgg tcgacgagga
     3961 cctgaccggt cacgataacc tgctgctgat cggacggctg ctcggcttca agcgacagca
     4021 ggcgaaggcg cgcgccgccg acctgctcgc cgcgttcgag ctcggcgacg ccgccgaccg
     4081 gcaggtcaag aactactccg gcgggatgcg gcgccgcctc gacatcgcgg cgagcatcgt
     4141 cgtgacgccg gagctgatct tcctcgacga gccgacgacc gggctcgacc cgcgctcgcg
     4201 cagccaggtc tgggagatcg ttcgcgcgct cgtcgccgac ggcacgacgg tgctgctctg
     4261 cacgcagtac ctcgacgagg ccgaccagct agccgacggc atcgcggtga tcgaccgcgg
     4321 caaggtgatc gccgagggga cgccggccca gctgaaggcc tcggtcggct ccggcgcgct
     4381 gcacatccgg ctgctcgacc ccgagcgccg tcacgacgcc gagcggcttg tcgcgcacgc
     4441 ggtcggagcg gccgtccagc tcgagcccga tcccgcggcg ctctcggttc ccgtggccga
     4501 cgccgcccgg gccgccgagg cggtcgcgga gctggcgcgg atggggatcg ggatcgccga
     4561 cttctcgctc gggcagccga gcctcgacga ggtcttcatg gcgctgaccg gccaccccgc
     4621 cgaggactcc ggcgcgaccg acccgaccgc gaaggaggcg gcatgagctc gaccgacgtg
     4681 acctcgacgg gcgccgcgga ggcgatgccc gacgccgccg ccctgcgcgc cgtcctcgcc
     4741 gccagcgagc gtccgccgcg gcccgggcgg ctcgccgcat ctctgacctt cggctggcgc
     4801 gggatgctca agatcaagca cgtccccgag cagctgctcg acgtgacgat cacgccggtg
     4861 atgttcgtcc tgatgttcac ctacctcttc ggcggcgcga tcgctggctc gaccggcgac
     4921 tacctgcagt tcatcctgcc ggggatcctc gtgatgtcgg tgctgttcac caccgtctac
     4981 tcgggcgtga cgctcaacac cgacatgacc aagggcgtcg tcgaccgctt ccgctcgctg
     5041 cccgtctggc gcccggcgcc gctcgtagga gcgctgctcg gcgacgccgt ccgctacctg
     5101 gtcgctggca cggtgatcat cgtgctcggc ctgatcctcg gctaccggcc cgaggcgggg
     5161 ccgggtgggg tggtggcggc gctcgcgctg gtcgtcgtct tcgcgttcgg tctctcgtgg
     5221 gcgttcacga cgctcggcct ggtgatgcgc gccccgaacg cggtgatgaa caccggcttc
     5281 atgctgctgt tcccgctcac gttcgtcagc aacgcgttcg tcgaggagga gacgctgccg
     5341 ggatggctgc aaaccgtcgt cgacgtcaat ccgatcagcc acctgatcac ggcggcgcgc
     5401 gggctgatgg acggcaacgc cgacggcggc gaggtcctgc tcgtattggc cgtcgcggcc
     5461 gggctgaccg cggtcttcgc gccgttgacc acgcgcctgt accgccgcgc cgcctgagga
     5521 ccggcgaacc ttcacacagc cgtgtcaagg ttggccgggg ttcgatagcc tggcgagcca
     5581 tggccgatcg cgactacctc caagccgtcc gtgaacgcgt cgtcgtcttc gacggcggca
     5641 tgggcgccac gctcgagcag ttcgacctga cccaggcgga ctacggcggg cttcagggca
     5701 agtgccacga ggcgctcgtg ctcaaccgcc cggacgtgat cgagggcgtc caccgctcga
     5761 tggtcgaggc cggggcggag gtcgtcgaga ccgacacctt ccaggcgagc aggctgaagc
     5821 tcgaggagtg ggggctggcc gagcacaccg tcgagatcaa ccggcgcgcc gccgagatcg
     5881 cccgcaaggc ggtcggcgag gaccgcttcg tcgccggctc gatcggacca accggcttcc
     5941 tgcccgcctc cgacgacccg acgctagggg acatccgctt ccgcgacct
//
LOCUS       DATABV010000014         7452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2816610, whole genome shotgun sequence.
ACCESSION   DATABV010000014 DATABV010000000
VERSION     DATABV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816610"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1105)
                     /locus_tag="VHF88_00530"
     CDS             complement(56..1105)
                     /locus_tag="VHF88_00530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2160291.1"
                     /translation="MTESSQPDPSGGPGGREPPDRILYVHSRKASFVAIDREILAERY
                     EIDDLYQPGRLPNPVRVVTGVLRADLVFCWFASWHSFFPITLAWLLRKPSVQIIGGFD
                     TANVPDIQYGHQRGGLRRWASRWIMRRATQLVTNSEYSRSEIAANTPFRPEDVTVVHH
                     GVPDPFGPLDTNTPRDRLALNVGAVDAGTLVQKGQIPFVEAASKLPDVRFVLAGKWLD
                     DSVDQLRQIAGPNVELTGWLSDADLHGLYRRASVYVQASRHEGFGLAVAEAMLAGCIP
                     VVMDVTAMPEVVGDAGILISSQSPDAVAAGIQEALQRGPDGARRARERITTEFPLQIR
                     RDGILTVVAETLHSR"
     gene            complement(1102..2991)
                     /gene="asnB"
                     /locus_tag="VHF88_00535"
     CDS             complement(1102..2991)
                     /gene="asnB"
                     /locus_tag="VHF88_00535"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX2160292.1"
                     /translation="MCGIGALLDPAGSAPPDAARRMAAALRHRGPDGEAIETHGPATL
                     VHRRLAIVDVAGGDQPLRSEDGAISLVANGEIYNHEALRAGLEARGHRFATRSDCEVI
                     VHLYEEHGDDCVRHLNGIFGFAIWDDRQRRLLVARDPLGVKPVYWHCAGRRVAVASEV
                     GALLAAGLVEPRVDRVALDHYLACRFVPAPRTLFEGVSKLPAASTLVATGDAPPRVAS
                     YRAAPGTPLADLDGDELESELAKRFGDAVERQMMSDVPYGAFLSGGVDSAAIVAAMAQ
                     RSGTPPQTFTIGFPGYGDALDERGYAAETARAVGTDHHAIATERGDYLDELATAVRRL
                     EEPCGIPSAPALMQLSRFAGESIKVALSGQGADEPHGGYGRHQAALALGAARRLGPLA
                     APIRAAAALMPGRERVRRAARLLGDMPDADRLVRLVEITGEGLRSELVGGAGEQAALE
                     RSALASDVLGDVPDRPLVERALYLDTHMFLPDSLLICGDKMSMTASLELRVPFLDVEL
                     LRFVERIPAKKRVRPRSGKHLHRKAMTRTLPPGLGHRPRHGFASPYDHWLRESVGTEV
                     EQRYGAGGPLAELIDPATVTRLVAEHRAERADHKAILFCLLELSQWHRTFIQDVAPAE
                     QVTGS"
     gene            3103..3333
                     /locus_tag="VHF88_00540"
     CDS             3103..3333
                     /locus_tag="VHF88_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160293.1"
                     /translation="MYRQLGLVHAHLIAMSGRLRQQAGQGTVEYVALILLVALIMAGV
                     VAAMSNFETDEGKELGDVILGKIKDAVRKVEF"
     gene            3378..4214
                     /gene="murI"
                     /locus_tag="VHF88_00545"
     CDS             3378..4214
                     /gene="murI"
                     /locus_tag="VHF88_00545"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX2160294.1"
                     /translation="MPKRDLPVAVFDSGVGGLTVLHECLVSLPHEDFLYLGDTARFPY
                     GTRAPEELRAFARELAGILLDEGAKLIVVACNSATSAALPGLRDELAGRVPVVSVVSP
                     EARLAAATTRNGRVGLLATPATVASGAYERALAAASDGRATLHAVPSAELAPIIQEGG
                     EIDERTVATVERACEPLRAAGVDTVILGCTHYPLVRPAIQRCLGRGVEIVTSGEAIVE
                     EVEHELDAAGSAAPRDRRGDYRFLATGDVAEFRRLGTRFLQLPIAAVGHVDVASPAEV
                     AA"
     gene            4211..4942
                     /gene="rph"
                     /locus_tag="VHF88_00550"
     CDS             4211..4942
                     /gene="rph"
                     /locus_tag="VHF88_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX2160295.1"
                     /translation="MRVGGRAADELRPIRIEPGFVRSATGSALIEAGGTRVICTASVD
                     ESVPRWMKGQGKGWVTAEYGMLPASTGDRKQRDVSKGRPDGRTVEIQRLIGRSLRGVI
                     DFEALGERTVWIDCDVLEADGGTRCASITGGYVALELALARLVSAGKLKALPLTGSIA
                     AVSCGVVAGEPLLDLDYPEDSTAEVDMNVVMTGEGGLIEVQATGERTPLSRTSLDALL
                     ALAAGGIEELRAAQRAAVDGALAAT"
     gene            4954..5538
                     /gene="rdgB"
                     /locus_tag="VHF88_00555"
     CDS             4954..5538
                     /gene="rdgB"
                     /locus_tag="VHF88_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX2160296.1"
                     /translation="MRLVLASRNAHKLRELNLLLTPHELVALPDSVELPPETGATFVE
                     NALVKARTAASATGMTAVADDSGIEAAALGGAPGIYSARFAGEDASDEDNLAKLLREV
                     PPDGDTRVAYVCALVLVEPGGRELVAEQRCHGRLTHSPRGEGGFGYDPAVLPDDYDDG
                     RTMAELTPAEKDAISHRGRAARELLSLIDGAAAR"
     gene            5535..6416
                     /locus_tag="VHF88_00560"
     CDS             5535..6416
                     /locus_tag="VHF88_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2160297.1"
                     /translation="MNDAQTGRKTRAAALSIVSNTLLIALKLAAGVITGSIAIITEAI
                     HSSVDLIASIVAFVSVRKAGVPADADHPYGHDKIENLAAVIEGLLILVGAGIIVFESV
                     RRLFDPPAVESLGIGIAVIGFSVVANLVVSTYLYRQARATESPALEGDAAHLRTDALT
                     SAGVLVGLLLVELTDFEWVDPLTALVVAVAIVVAGMRILMRSSRVLVDEVLPDHELAA
                     VREALEVEHRAPEIAGYHKLRARRAGSRRYIDLHVQFHEGTTLDRAHAVSHELQEEIR
                     ERLDRADVLIHLEPERR"
     gene            complement(6510..6650)
                     /locus_tag="VHF88_00565"
     CDS             complement(6510..6650)
                     /locus_tag="VHF88_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160298.1"
                     /translation="MPLPRISWMVTVAVCLVAAILLLISGYSGYAAVLVAIGAAAAVN
                     LL"
     gene            6669..6992
                     /locus_tag="VHF88_00570"
     CDS             6669..6992
                     /locus_tag="VHF88_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160299.1"
                     /translation="MVAARLADSSTDVIAAVVLDAAGGLVEVSGIEREQARELADLAR
                     QLVERADSVSPDPVAQIEVQTIGGAVFAVRDSRHVLACVTRRTALPALVLYDLRHALM
                     ELAPA"
     gene            6989..7216
                     /locus_tag="VHF88_00575"
     CDS             6989..7216
                     /locus_tag="VHF88_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160300.1"
                     /translation="MRERPKGAVARATAVAEGVAGAMRRRQREREPRVLLYRAPGDPC
                     LLPPGAKGQDDLIDLADQIVTLAKSANSGNS"
BASE COUNT         1030 a         2534 c         2859 g         1029 t
ORIGIN      
        1 atgacttgga ggcgctggcg gagtcggggg tggcgatgat ggaggggttg gcgggctagc
       61 gggagtggag cgtctcggcg acgacggtga ggatgccgtc gcggcggatt tgcagcggga
      121 actcggtggt gattcgctcg cgcgcgcggc gggctccgtc ggggccgcgt tggagggctt
      181 cttggatgcc ggcggcaacg gcgtcggggg attgagagga gatgaggatg ccggcgtcgc
      241 cgacgacctc gggcattgcg gtgacgtcca tgacgacggg gatgcagccg gcgagcatcg
      301 cctcggcgac tgcgaggccg aagccctcgt ggcgggaggc ttggacgtag acggaggcgc
      361 ggcggtagag gccgtggagg tcggcgtcgg agagccatcc ggtgagctcg acgtttgggc
      421 cggcgatctg ccggagctgg tcgacggagt cgtcgagcca cttgccggcg aggacgaagc
      481 ggacgtcggg gagcttgctg gcggcttcga cgaaggggat ctggcccttc tggacgagcg
      541 tgccggcatc gacggcaccg acgttgaggg cgagccggtc gcgcggggtg ttggtgtcga
      601 gggggccgaa ggggtcgggg acgccgtggt ggacgaccgt cacgtcctcg ggacggaagg
      661 gcgtgttggc ggcgatctcg ctgcggctgt actcggagtt ggtgacgagt tgggtggcgc
      721 ggcgcatgat ccagcgggag gcccagcggc ggaggccgcc gcgctggtgg ccgtactgga
      781 tgtcgggcac gttggcggtg tcgaagccgc cgatgatctg gaccgacggc ttgcgcagga
      841 gccaggcgag ggtgatcggg aagaagctgt gccaggaggc gaaccagcag aagacgaggt
      901 cggctcggag gacgccggtg acgacgcgca cggggttcgg caggcggccc ggctggtaga
      961 ggtcgtcgat ctcgtagcgc tcggcgagga tctcgcgatc gatcgcgacg aagctcgcct
     1021 tgcggctgtg gacatagagg attcggtcgg ggggctcgcg ccccccgggc cccccgctgg
     1081 ggtctggctg gctggactca gtcatgagcc ggtgacctgc tcggcggggg cgacatcctg
     1141 gatgaaggtg cggtgccatt gggagagctc gagcaggcag aagaggatcg ccttgtggtc
     1201 ggcgcgctcg gcgcggtgct cggcgacgag gcgggtgacg gtggcggggt cgatcagctc
     1261 ggcgagcggt ccgccggcgc cgtagcgctg ctcgacctcg gtgccgacgg actcgcgcag
     1321 ccagtggtcg taggggctgg cgaagccgtg tcgggggcgg tggccgagac cgggcggcag
     1381 cgttctcgtc atcgccttgc ggtggaggtg cttgccggag cggggacgga cgcgcttctt
     1441 cgccgggatg cgctcgacga agcgcagcag ctcgacgtcg aggaacggca cgcgcagctc
     1501 gaggctcgcg gtcatcgaca tcttgtcgcc gcagatcagc aggctgtccg gcaggaacat
     1561 gtgggtgtcg aggtagagcg cgcgctcgac cagcgggcgg tctgggacgt cgccgaggac
     1621 gtccgaggcg agtgcgctgc gctccagcgc agcctgctcg ccggcgccgc cgacgagctc
     1681 ggagcgcagg ccctcgccgg tgatctcgac caggcggacc agccggtcgg cgtcgggcat
     1741 gtcgccgagc agccgggcgg cgcggcgtac gcgctcgcgg ccgggcatca gcgcggcggc
     1801 ggcgcggatc ggggcggcga gcgggccgag gcggcgagcg gcgccgaggg cgagcgccgc
     1861 ctggtgacgg ccgtagccac cgtggggctc gtcggcgccc tgtcctgaga gcgcgacctt
     1921 gatcgactcg ccggcgaagc gggagagctg catcagcgcc ggagcggagg ggatcccgca
     1981 gggctcctcg agccgccgga cggcggtcgc cagctcgtcg aggtagtcgc cgcgctcggt
     2041 cgcgatcgcg tggtggtcgg tgccgaccgc gcgggcggtc tcggcggcgt agccgcgctc
     2101 gtcgagcgcg tcgccatagc ccgggaagcc gatcgtgaag gtctggggcg gcgtgccgga
     2161 gcgctgggcc atcgccgcca cgatcgccgc cgagtccacg ccgccggaga ggaaggcgcc
     2221 gtacgggacg tcggacatca tctggcgctc gacggcgtcg ccgaagcgct tcgcgagctc
     2281 gctctcgagc tcgtcgccat cgaggtcggc cagcggcgtg cccggcgcgg cgcgatagct
     2341 cgcgacgcgc ggcggcgcgt cgccggtggc gacgagcgtc gacgcggcgg gcagcttcga
     2401 cacgccctcg aacagcgttc gcggggcggg cacgaagcgg caggcgaggt agtggtcgag
     2461 cgcgacccgg tcgacgcgcg gctcgactag cccggcggcg agcagcgcgc cgacctcgga
     2521 ggcgaccgca acccggcggc ccgcgcagtg ccagtagacc ggcttgacgc cgagcgggtc
     2581 gcgcgcgacc agcaggcggc gctggcggtc gtcccagatc gcgaatccga agatgccgtt
     2641 gagatggcgc acgcagtcgt cgccgtgctc ctcgtagagg tgcacgatca cctcgcagtc
     2701 cgagcgggtg gcgaagcggt ggccgcgcgc ctcgagcccc gcgcgcagcg cctcgtggtt
     2761 gtagatctcg ccgttcgcga caagcgagat cgcgccgtcc tcggagcgca gcggctggtc
     2821 gccgcccgcg acgtcgacga tcgcgagccg gcggtggacc agcgtcgccg gcccgtgcgt
     2881 ctcgatcgcc tcgccgtcgg ggccgcggtg acgcagcgcc gcggccatcc gccgcgcggc
     2941 gtcaggaggc gccgagccgg cagggtcgag gagggcgccg atgccgcaca cgcgccgcat
     3001 gatcgcagcg ccccgtcaca tagctgtcac tgaagcgcct cgaaccgcgc gtcgcgtcgg
     3061 gcgagggtga ccgtccctca accgatccac ggaggtcgac tcatgtatcg ccagctcgga
     3121 ctggtccacg cccacctgat cgccatgtcc ggccgcctgc gccagcaggc cggtcagggc
     3181 acggtcgagt acgtcgcgct gatcctgctc gtcgccctga tcatggccgg cgtcgtcgcc
     3241 gcgatgagca acttcgagac cgacgagggc aaggagctgg gcgacgtgat cctcggcaag
     3301 atcaaggacg cggtgcgcaa ggtcgagttc tgagcggcgg acggtcacgc cgccgacccg
     3361 catgcgagca tgcagctgtg ccgaagcgcg atctgccagt agccgtgttc gactccggcg
     3421 tcggcggcct gaccgtcctc cacgagtgcc tcgtgtcgct gccgcacgag gacttcctct
     3481 acctcggtga cacggcgcgc ttcccgtacg gcaccagggc gcccgaggag ctgcgcgcct
     3541 tcgcgcgcga gctggccggg atcctgctcg acgagggggc gaagctgatc gtcgtggcct
     3601 gcaactcggc gacctcggcg gcgcttccgg gcctccgcga cgagctcgcc gggcgggtcc
     3661 cggtcgtgag cgtcgtctcg ccggaggcgc gcctcgcagc cgctaccacg cgcaatggtc
     3721 gcgtcggcct gctggcgacg ccggccacgg tcgccagcgg cgcctacgag cgcgcgctgg
     3781 ccgccgcctc ggacggccgg gcgacgcttc acgcagtccc aagcgccgag ctcgcgccga
     3841 tcatccagga gggcggcgag atcgacgagc gcaccgtcgc caccgtcgag cgtgcctgcg
     3901 agccgttgcg cgcggccggc gtcgacaccg tgatcctcgg ctgcacccac tacccgctcg
     3961 tcaggccggc gatccagcgc tgcctcggac gcggcgtcga gatcgtcacc tcgggcgagg
     4021 cgatcgtcga ggaggtcgag cacgagctcg acgccgccgg cagcgccgcg ccgcgcgacc
     4081 gccgtggcga ctaccgcttc ctcgccaccg gcgacgtcgc agagttccgg cgcctcggca
     4141 cccgcttcct gcagctcccg atagcggcag tcggccacgt cgacgtggcc tcaccggcgg
     4201 aggtggcggc atgagggtgg gcggccgcgc cgccgacgag ctgcggccga tccggatcga
     4261 gccgggcttc gtgcggtcgg cgaccggctc ggcgctgatc gaggcgggcg gcacgcgcgt
     4321 gatctgcacc gcctcggtcg acgagtcggt gccgcgctgg atgaagggcc agggcaaggg
     4381 ctgggtgacg gccgagtacg ggatgctgcc cgcctcgacg ggcgaccgca agcagcgcga
     4441 cgtctcgaag ggccgccccg acggccgcac cgtcgagatc cagcggctga tcgggcgttc
     4501 gctgcgcggc gtgatcgact tcgaggcgct tggcgagcgc acggtctgga tcgactgtga
     4561 cgtgctcgag gccgacggcg gcacgcgctg tgcgtcgatc accggcggct acgtagcgct
     4621 cgagctggcg ctggcgcggc tggtgagtgc cggcaagctg aaggcgctgc cgctgaccgg
     4681 ctcgatcgcg gccgtctcct gcggcgtcgt cgccggcgag ccgctgctcg acctcgacta
     4741 ccccgaggac tcgaccgccg aggtcgacat gaacgtcgtg atgaccggcg agggcggtct
     4801 gatcgaggtc caggcgaccg gcgagcgcac accgctctcg cgcacctcac tcgacgcgct
     4861 gctggcgctc gccgccggcg ggatcgagga gctgcgtgcc gcccagcgcg ccgcagtcga
     4921 cggagccctc gcagcgacct gagcgaggat gacgtgcggc tagtgctcgc atcgcgcaac
     4981 gcgcacaagc tgcgcgagct gaacctgctg ctgacgcccc acgagctggt cgcgctgccc
     5041 gactcggtcg agctgccgcc cgagaccggc gcgacgttcg tcgagaacgc gctcgtcaag
     5101 gcgcgtaccg ccgcgtcggc gaccggcatg accgcggtcg ccgacgactc cggcatcgaa
     5161 gcggcggcgc tcggcggcgc gccgggcatc tactccgccc gcttcgccgg cgaggacgcc
     5221 agcgacgagg acaacctcgc gaagctcctg cgcgaagtcc cgcccgacgg cgatacgcgc
     5281 gtcgcctacg tctgcgcgct cgtgctcgtc gagccaggcg gtcgcgagct ggtcgccgag
     5341 cagcgctgcc acgggcggct gacgcactcg ccgcgcggcg agggaggctt cggatacgac
     5401 ccggcggtct tgccggacga ctacgacgac ggccgcacga tggcggagtt gacgccggcc
     5461 gagaaggacg cgatcagcca ccgcggtcgc gccgcacgcg agctgctgag cctcatcgac
     5521 ggggccgcgg cgcggtgaac gacgcccaga cgggccgcaa gacgcgcgcg gcggcgctgt
     5581 cgatcgtctc gaacaccctg ctgatcgcgc tcaagctggc cgccggggtg atcaccgggt
     5641 cgatcgcgat catcaccgag gcgatccact cgagcgtcga cctgatcgct tcgatcgtcg
     5701 ccttcgtgtc ggtccgcaag gcgggcgtgc cggccgacgc cgaccacccg tacgggcacg
     5761 acaagatcga gaacctcgcc gcggtgatcg aggggctgct gatcctcgtc ggcgcgggga
     5821 tcatcgtctt cgagtccgtc cggcggctct tcgacccgcc ggcggtcgag tcgctcggca
     5881 tcggcatcgc ggtgatcggc ttctcggtcg tcgccaacct ggtcgtgtcg acctacctct
     5941 accgccaggc gcgtgccacc gagtcgccgg cgctcgaggg cgacgctgcc cacctgcgca
     6001 ccgacgcgct gacctcggcc ggcgtgctcg ttgggctgct gctcgtcgag ctgaccgact
     6061 tcgagtgggt cgatccgctg accgcgctgg tcgtcgccgt cgcgatcgtg gtcgccggga
     6121 tgcggatcct gatgcgctcc tcgcgcgtcc tcgtggacga ggtgctgccc gaccacgagc
     6181 tcgccgccgt ccgcgaggcg ctcgaggtcg agcaccgcgc tcccgagatc gccggctacc
     6241 acaagctgcg cgcgcgccgg gccggcagca ggcgctacat cgacctccac gtccagttcc
     6301 acgagggcac cacgctcgac cgcgcccacg ccgtctcgca cgagctccag gaggagatcc
     6361 gcgagcggct cgatcgcgcc gacgtcctca tccacctcga gccagagcgg cgatagccgt
     6421 cgcatggcgg cgtcatcggc ctctcgtgtg ctggcgcaca cgtcgaggcc tgcttccttg
     6481 ccctgctcgg ctctcgccgc tctgcggcgt tagagcaggt tgaccgcggc ggcggcgccg
     6541 atggcgacga ggacggcggc gtagccggag tagccgctga tcaggagcag gatcgcggcg
     6601 accaggcaga cggcgacggt gaccatccac gagattcggg gcagcggcac gcggctattg
     6661 tgactgcgat ggtcgccgcg cgactggccg acagctcgac ggacgtcatt gcggccgtcg
     6721 tgctcgatgc ggccggcggg ctggtcgaag tcagtggcat cgagcgcgag caggcgcgcg
     6781 agctggcgga cctcgcgcgg cagctcgtcg agcgcgccga ctcggtctcg cccgatccgg
     6841 tcgcgcagat cgaggtccag acgatcggcg gcgcggtatt cgcggtgcgc gacagccgcc
     6901 acgtgctggc ctgcgtcaca cggcggacgg cgctgccggc gctcgtcctc tacgaccttc
     6961 gccacgcgct catggagctg gcgccggcgt gagggagcga ccgaagggag cggtcgctcg
     7021 cgcgaccgcc gtggcggagg gcgtggccgg cgcgatgcgc cgtcgccagc gcgaacgaga
     7081 gccccgcgtc ctgctctaca gagcccctgg agacccgtgc ctgctgccgc cgggcgcgaa
     7141 gggacaggac gacctgatcg acctcgccga ccagatcgtc accctggcga aatccgcaaa
     7201 ttccgggaac agctagcgcg aaacccgttt ccagagcggt tctcggagcc ggtcgtccgc
     7261 gcccattgtt aggttcgccg cactgttcca gtccctccaa aggaggaaac aaaggtgacg
     7321 aagtcagagt tcgtcgatca ggtggccgcg gagtccggcc tctccaaggg tgacgccggg
     7381 accgccgtcg acgcggtcct gacggtcatc gaggacaccc tgaagcgtgg cggagaggtc
     7441 agcttcaccg gc
//
LOCUS       DATABV010000015        47832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1291427, whole genome shotgun sequence.
ACCESSION   DATABV010000015 DATABV010000000
VERSION     DATABV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 47832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47832
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291427"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VHF88_00580"
     CDS             <1..517
                     /locus_tag="VHF88_00580"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX2160301.1"
                     /translation="APPPSPGRDETPLERTDRNLSELLGELRVALPGVQVLFAFLLIV
                     PFNSRFDDATPFQRDVYLATLICTAFASALLIAPTMHHRLEFRHGDKEHLVKLSNRLA
                     IAGLTLVALAMTGVTVLVTDFVFDTVEAVATTGAVALGFATLWFAMPLQRRRTLSRRR
                     DSKPAQSTDDG"
     gene            complement(514..1611)
                     /locus_tag="VHF88_00585"
     CDS             complement(514..1611)
                     /locus_tag="VHF88_00585"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX2160302.1"
                     /translation="MRRLLPILACLGLVLGGAAPATAANSRDGWGLFVADDDWDFANP
                     GALPALGGDFAKLGPRVFRLQTIWNTVDRPEWMTRTQAMIAEARLQGAQQVILTLRSN
                     NPANVGPEGYFPTADEYRAKVEPLVQQLAPVVDVWGPANEPNIAWRPKEDPAGQAPLP
                     ALTLAGYYLAFQEVVRRHDPSALITSPDFLDAGSLASFTEYVTTYSTAAGGGWGDVVA
                     LHPYGDVKRAVEPTAPATFTDAFAALVPPDKDIWVTEVGAHHEGDSAAQAARVEWIAE
                     VLANHPRVKRVAYYNMRGGGPDWDTGLLDGDFSRRPAWYAWCAATHGDDPRHPDCAPV
                     LPPGGDGPGCIAYFIEYMVLETHDWSGVRCV"
     gene            complement(1633..2340)
                     /locus_tag="VHF88_00590"
     CDS             complement(1633..2340)
                     /locus_tag="VHF88_00590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2160303.1"
                     /translation="MASADTADARPGRLLPDTLRDEMSRLNGEIRVDEGGGASALKVL
                     AIADAIVANRLRHVVEIGVYRGRLLLPLGILMRALDGEAIGVDPYSAAAAVQSDSHGR
                     SIDLASWAQQVDWQGIHDDVQARIATLGIDCHCRIVRKRSEDAADDFAPASIDLLHVD
                     GNHDRAAVERDVALFLPKVRPGGFVVMDDAAWPSVRPTCERLADEHELVLQLFDGTGM
                     LDGVGGNDFALFRLTGA"
     gene            2386..3552
                     /locus_tag="VHF88_00595"
     CDS             2386..3552
                     /locus_tag="VHF88_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2160304.1"
                     /translation="MRIPGPTAAGESASSPLRFQRPSLPSGDAIERYLSLAREERWFS
                     NGGPCWKLLRDRLSERVGAYCVPVASGTVGLMAAVAAVAGDAPPHSKALLPSFTFAAT
                     VQAAIWTGLDPLLLDVDSAGWHMDCAQLEEELHAAGATAALALVVSSFGTPPPPATRQ
                     RWEQACRAAGVPLVIDSAAGFGAIAADGVPIGAQGDVEVVSFHATKPFAIGEGGAVFS
                     HDARLIERIERTVNFGLDRDRSVVDPRGLNGKMSELHAATALAVLDELDSILAARRGA
                     AAEVRERAGADVSWQAESERSAWQFVPVAFADARARGEAQERCAARIETRVYYEPLHH
                     MPAYRELPHASGGLSVTERLAQRLLCLPMANDLTSSEAVRIATAIRGRAAQPAG"
     gene            complement(3537..4187)
                     /locus_tag="VHF88_00600"
     CDS             complement(3537..4187)
                     /locus_tag="VHF88_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEX2160305.1"
                     /translation="MRAVVYGSRSDGHAGVVVELFGADSGLELVGLLDDRPENRGNRV
                     AGLEVIGDRGALGRLVGEGVEAVVLGFGVARGRAKVVEAVEAAGLALPVLIHPSAHVT
                     ASATLAAGCQVLPNATVGAGASLGRGVLINSGAIVEHDARLADAVVIDPGAVLAGRVT
                     VECESEVGSGAVIRPDVCVGGGAVVGAGAAVVSDVAAGSTVVGVPARPLRRAQPAG"
     gene            complement(4190..5425)
                     /locus_tag="VHF88_00605"
     CDS             complement(4190..5425)
                     /locus_tag="VHF88_00605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2160306.1"
                     /translation="MQFADLVDFYGLPARDRGQERPRLVQPSRAAAALGRARGRLRRG
                     RRQRLAFLDATFPWQRSGFRYHEAAAIHDLLPDTLFFSMWEMTDPFPAPVHPLAELPT
                     IAPGAGITDAYGVFQIFLEGLCGMEPTTDEEAGHPFRPFDMSAVFERSGIRLHGSVYP
                     GGGFTPTPHGLARVRELAARLDTTFSYVREVLDTVPGVTEVDQAFTETRFYVVSSERW
                     ERPRPLTCLFAADAPPRKGLDVALEAFRDLEPEAFHLHVVGPHEQRRDELPPGLATFH
                     GWLSPAELRELHREVHVFLSPVKAEAAGPPGSYQGVTDGFPTQAAADAMSSGCLLVSA
                     NPAADHRVLEPEVHYLERVARPAAVRATLERLAADPAWARRVASAGSERLRSRMDVRH
                     GAAQKLAAMGFSSDIAAVR"
     gene            5510..6397
                     /locus_tag="VHF88_00610"
     CDS             5510..6397
                     /locus_tag="VHF88_00610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2160307.1"
                     /translation="MTDPEGQLDALARRLEELASELAAVRELAARAYDDTPALARRLF
                     ERRRSPEWQDAYAAGPLVSVRIGTYRGGDLLFDRTLASVLAQSYEHWEAIVVCDGQEP
                     ETAARIAALGDPRVRCFQRPRPGPYPEEQRARWHVAGSFPFNEAVGRARGAWIAPLDH
                     DDEWTADHLELLLEQARRTRAEVVYGAFRALVADAGETWFGAWPPRQGDFGFQAAIYH
                     AGLADALYDANAHLLDEPGDWNLARRLLEAGVRWDFVDRVVGTYYVDADAATLDWWRE
                     RVASRGAHPDSAASDLPPD"
     gene            6408..8270
                     /locus_tag="VHF88_00615"
     CDS             6408..8270
                     /locus_tag="VHF88_00615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2160308.1"
                     /translation="MPGLPRICIVSASGQNVFFAEILEAFGDALRDHGAVVEQSVDCF
                     PPAAEDLVCLYVPHEYHPLVQEPAHPTPVQLGRSVALCTEQPGTQWFELATDVAARAG
                     GVVDINALGARELERRGIAVEHAPLGYVPSWDAWHGRETGERSIDLAFLGSHTDRRAG
                     ALARCAPVLRGRRAAINMVESVQPHIAGSGSFLSHDRKWRLLADTKAILNVHRTPLGY
                     MEWHRIVGAALNGCVVLSEHSLGVEPFVPGEHYISASYDTLPEALEGLLSDTDLQRAT
                     RQAAYELLRERMPMSTAVDALLAAAERAARGPLRTSGVASVPGVPMPKPPPPRQPDWA
                     AFATEVGDQLPVRMALKHLVVHTRNLERRIEKLASGDGEQPAEDVVERLGPDVDDVRV
                     SVLLTVHNYADYVGQALHSVALSDLREIEVVAVDDASTDDSVDAIRTACAEVPWLPVT
                     LVRRGRNGGLPAARNLALAHARADLLFVLDADNAVLPEGIGRLAQALDDDREAAFAYG
                     IIEAFSTSGPVGVMNWLDWDPDRLRYGNYIDAMSMIRRSTLEAVGGYSTEPALYGWED
                     FALWCAIASNGLRGVRVPDFVARYRVSGHSMISLTNIDSSAAWATLLRKYPALA"
     gene            8280..9203
                     /locus_tag="VHF88_00620"
     CDS             8280..9203
                     /locus_tag="VHF88_00620"
                     /inference="COORDINATES: protein motif:HMM:NF017345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEX2160309.1"
                     /translation="MTFVHEQAIVDTSSLGEGSRVWAFAHILEGARIGRDANVCDHVF
                     IENDVVLGDRVTVKSGVQLWDGARVEDDVFIGPNVSFVNDRFPRSKQHPDRFLTTTLR
                     DGCSIGAGATILGGVTVGTGAMVGAGAVVTRDVPPFAIVTGNPARIRGYAGADRAPFE
                     DADPGSASPEPGADVGGVRLISLHSVTDLRGSLSVGEVGAQLPFVPKRIFFVYDVPTA
                     EVRGEHAHRRLEQVLICVRGTISVVVDDGRARAEYKLDRLDRALHIPPKVWAIQYAYS
                     RDAVLLVLASEEYDSDEYIRDYGEFRQITAS"
     gene            complement(9190..10131)
                     /locus_tag="VHF88_00625"
     CDS             complement(9190..10131)
                     /locus_tag="VHF88_00625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2160310.1"
                     /translation="MSAPANSLVVPIYRNAAMIDALVTAVGDIAEQVVDGFEAVFVID
                     GSPDDSRERLLAALDAAPFRWRLVSHARNFGSFAALRTGMAIADGRYIAVMAADLQEP
                     PELVIEFFRRLASGEVDIVAGERASRDDRGDGASSLYWRLYRRFVMADIPPDGVDVFA
                     CTAQVRDVLTSLEDQNTSLVAQLFWVGFRRELIPYHRRPRPGKSGWTLRKKLRYLSDS
                     VFAFTDLPVRMLWAAGLVGLALGTVIAALVAVLRLTGAIDVPGYAPTILVVIFFAGLN
                     CLGLGIIGSYVYRAYEAVKGRPGAIVSEVVDPSVSSR"
     gene            complement(10128..11258)
                     /locus_tag="VHF88_00630"
     CDS             complement(10128..11258)
                     /locus_tag="VHF88_00630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2160311.1"
                     /translation="MSTVRPDTILANDFAAQWDEVREDALAAIDRVGSSGWLVLGAEV
                     EAFERELGAWWGVEHAVGVASGLDAIEIALRCLGIGPGDRVLTTPLTAFATTLAIMRA
                     GAVPIWSDVDESGSMDLAAADEVLAADPSIRAVVPVHLYGHPLDPAALRAMAERHAVV
                     VVEDCAQSAGAARAGAPTGRAGVAAATSLYPTKNLGAMGDGGVLLTDDAELAERARRL
                     RNYGQSERYRHVEPGLNSRLDELHAAVLRSALLPRLDGWLRRRAEVAERYVEALQDSP
                     VLSAVRPTGGTSANHLFPVEVIEGEAERHAEALEAAGVSVGRHYPVLCPDQPACAGTG
                     STATALPTARRLAERELSLPLHPHLADADVERVIDACGSLAA"
     gene            complement(11255..11692)
                     /locus_tag="VHF88_00635"
     CDS             complement(11255..11692)
                     /locus_tag="VHF88_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX2160312.1"
                     /translation="MRDWLQRNVLRPPRDHREEMLRFVVVGGCGYVLAMALYAGEIAL
                     GVSAYAAVPPVFVANGLFNFTLNRHWSFPRSGRAVHRELGRFTVVAAASLIVNYVALY
                     LLHGVGGLAAVPAQALAVVIATPVGFLGSKFFSFAQSGAETAR"
     gene            complement(11734..12174)
                     /locus_tag="VHF88_00640"
     CDS             complement(11734..12174)
                     /locus_tag="VHF88_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160313.1"
                     /translation="MAGLFARHVINGFDQRYETPYIEQVDTGSQRWMRVLGFLLVFLA
                     LARVAWPAARRSLGPSRWRYPVGLLLCGAASLPSAVETRFLLPAYVLAYLLALAPGWP
                     NPLAPRATARQRSVTVAGLAVAFALYMAVVLHVVGSTTEQLRFV"
     gene            complement(12144..13136)
                     /locus_tag="VHF88_00645"
     CDS             complement(12144..13136)
                     /locus_tag="VHF88_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160314.1"
                     /translation="MAAIDIVPASMYLRTPAVAAFGVAFAAALTIALLHGEKPFYYDA
                     GHYWELGDSFVVDGDFSLLNFDSPLRGYLLALIYHGLAGVADGLTWSPSSVVKIFNAA
                     IVAAISTVLAPSLARLAWPELTWGFWRRLGLAALVLVFWRSYMSFPLSDFPALAAVML
                     ALVAVGRAEAPGWMLVAGASSAAAINMRPAYILVVPAVVGLLAIAWWKARAAPRRSTA
                     RGLLGVAALLVGFAAISLPQSLATDRHHDSSSFVPGAVADLSSLQFTAGLTLQRYETF
                     VGSDRPGGRMMYFDPTGQRILQSRDDAVVDDAGEYARIVVSHPIIWRASSRGMS"
     gene            13150..14859
                     /locus_tag="VHF88_00650"
     CDS             13150..14859
                     /locus_tag="VHF88_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160315.1"
                     /translation="MPTRTPPSRPHTAASRPVAPPLARLRARAGDSKHLPVAQTAIVA
                     LLLGLIATVVYGPHAIHGGFLSDAWAARTLYEFAPQDDFLGRLGHLLSQPNVSPRPLQ
                     GVYVAVQNAVFGPHMGLWLTWQGAMSVVMSLVLYLLLRELRFSWQDAGAIAVLVLIFP
                     AASSIRLWTAPMSALTISLALLGFLLALRAFRRDGARRIALHGLSLAVFAASLLLYEV
                     ALSAMLASVLVYRLCVPWRAAGARWLVDVVVLVPLAALVTRSESSSWEAQTFDGMWRH
                     ATVFWDEAQTLLTSVVLPFGTDRWYALALIALIPAIAVLLARQLPSSDPARADLRRWL
                     LTVLAGLAIVALGYAMFVPAMDYYTPLRPGIANRINAVPSIGWVLVLYGLAMLAATIA
                     FRGQRQRRLGASALALVACAIVAASWLESIDREAEAYMRAYSEGERVLQTVETLLPDP
                     PPHSVIWTFGQPVEIAPGIPVFGNTWDMTGSVQLMYDDPTITSYVGHPGTTFDCERER
                     IVAGGPAYLVPSSPGAQPLSSPYGPTYFVDTVDGRLQRIRSRAECRRARVSFELSPAL
                     APA"
     gene            complement(14863..15960)
                     /locus_tag="VHF88_00655"
     CDS             complement(14863..15960)
                     /locus_tag="VHF88_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2160316.1"
                     /translation="MRPPAVGRWRTSARTVPAASARQARLSTIASMEQAVHPERFDPR
                     DQQGTLIDTEHRGRYWWAAQAVRDKDVLDAGCGTGYGMALLASGGAASVSGVDIDPDA
                     VAEAGERFGDPSAVTRGDLLDLPFGDASFDVVVCFEAIEHVADGARTLGELHRVLRPG
                     GVLLVSSPNPDVYPSGNEHHVHEYRPDELAAAVGELFANVDAYRQHPWLATRIERANG
                     HGGPDGDISAHVRATTALDGGGETYGIVAASDAPLPPLESVVALGDAFEVSWWADHVQ
                     SVEAEAHRSVARATAAEQSATERLQDTSRALLDAEQALAQIPLMQSRVQEQLEENRQL
                     LSRLNALEGSLTWRMTEPVRRIKRRLSRGLR"
     gene            15927..17186
                     /locus_tag="VHF88_00660"
     CDS             15927..17186
                     /locus_tag="VHF88_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2160317.1"
                     /translation="MSATVQQPAASGMLSAATEPPAVSVGSVSKSFRLPHEQYSTLKQ
                     RVLHPRRSRTFSELHALEDVSFEIAQGEFFGIVGRNGSGKSTLLKCLAGIYGVDSGRI
                     DVRGRLSPFIELGVGFHPELSARDNVVINAIMLGLTRKQALDRFDEIIAFAELEEFVD
                     LKLKNYSSGMSVRLGFAVAIQVDADVLLVDEVLAVGDASFQQKCFDQFDRLKAEGRTI
                     VFVTHDMDSVRRFCDRAMLLERGNPVAIGEPDGIAAKYAEINFGVVGEDGAGGGTGLP
                     RARIARAWCETADGSAAETARDTFTAGRGEPCAACMEIEFSDPVEDPVFTIAFRNDVR
                     HTIYVARSDLDGPTGSFQAGQRATIRFRFPNWLAHSTYTLTPAVMTSDGRIALDQRID
                     ALTLLVDTNRVTGGVADLPTEVEVERS"
     gene            17183..18022
                     /locus_tag="VHF88_00665"
     CDS             17183..18022
                     /locus_tag="VHF88_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2160318.1"
                     /translation="MSVAEAVQTRGPSALGGDLRRALSLTWMLAVTDFKLRYFGSVLG
                     YLWTLMRPLMLFGVLYFVFTKVFSIGETVEHYPVYLLTSIVLFTYFSETTAGGVQCLI
                     GRENLLRKMHFPRIAIPVSVSLSALFNLALNLIAVLVFALASGVEPRLTWLQFPLLLL
                     ALTLLAIGFAMLLSVLYVRFRDIQPIWDVVLQITFYASPVLYVIGMLPDNIQREALVA
                     NPIAVILTQMRYAVLDSSAPTAAEMAGVDARLLIPAGLVVVVFCLGLWLFTREAPRIA
                     ENL"
     gene            18024..19301
                     /locus_tag="VHF88_00670"
     CDS             18024..19301
                     /locus_tag="VHF88_00670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2160319.1"
                     /translation="MARTTQRDAAQANGQPSDDELAAIEREHVEEVARAHAALAEAQD
                     RSYWLDRWGVDLNEWMRRPGASELRAAVRALRGVRRAAIKARRQGKAKLVKARREMAA
                     EREQAVPAESAPKLFERSLTPKRLESAPATKLVFDRLSETDVDAIRSSCNVNENAIVD
                     SADPMNQLRLLIGFGAHHGIPAVVERTGLSGDMPPEDVHAMARGSMAIAGSLYYADLV
                     ADGLETAGMTLEDGMRCLDFGCSSGRVVRALAAAYPGCEWRGCDPIEGAVTWASENLP
                     AIDFVHSPERPPLPYGDGELDAAFAISIWSHFDEPDAVAWLAEMRRLIRPGGRLLLTT
                     HGYQTIAHDEGHGLRQTSQLQEVHAALYDRGFWFKNEFGEGGDHGVDNSGWGTAFLTP
                     EWLLWATVRDWRVVGFAPGRVEDNQDLYVLERR"
     gene            19316..21481
                     /locus_tag="VHF88_00675"
     CDS             19316..21481
                     /locus_tag="VHF88_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2160320.1"
                     /translation="MSTSVSVVIPVKDGARHLERLLAAVRRQGEGVELLAIDSGSRDG
                     SVELARAGGATVHEIAPEEFGHGRTRNLAMELATGDVVCFLTQDAVPEPGWLAAHLAA
                     YELDPEVGAVYGPHLPWPDTSPMIARELDEFFASMSPDGKPTLQRAGDLAFLSNVNAS
                     YARRCWEEIRFEDLAYAEDQAFGRAMLDAGWVKVFHPGAAVRHAHDYGPLEFARRYFD
                     EYRGLRETAGHVEPLKPGRVLADVRGGVAADRRWMAERDWPQSRRAVWTARSLLHHGS
                     RKAAAAAATRPGRLPAGVERRLSLEGRASVARANRRGDGPPDRATADARSRAPVAPVP
                     ARYADVLRYAKEGAVPLAPRNSTAHERARLHLAFVVPPFRRGSGGHSTIFGLIARLER
                     MGHTCTVWIVDPGGNGHIDRPAVTRRDIVEWFTPISAPVFAGFDAWHGADVAVATGWD
                     TAHPVAMLPGCGARAYLVQDHEPEFFATSAERIWAEQTYDLGLYPICASRWLRDLLAE
                     QHGCEGAYFHLGVDHDVYRPQDHVERRRDTIIFYARAATARRAVPLGLLALEELCARR
                     PDTRVIAFGQERPLKTSFQTISLGVAAPARLAAAYAEATAGLCLSLTNYSLIPQEMLA
                     CGLPCVDLAGGSSQTVFGADGPVVLADADPIALADALEALLDDERHWRAKSSAGIEFV
                     ADASWDTAAKQVEAGLREALRRAAMPDGAASAGRHSQAR"
     gene            complement(21478..23583)
                     /locus_tag="VHF88_00680"
     CDS             complement(21478..23583)
                     /locus_tag="VHF88_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160321.1"
                     /translation="MVCAPVLLHGDVTLAGYLLDTTVAFHLAGSDYLLEHARNFARLP
                     ESSFRTTMENYFGLSYPGGGQTLLGGGSRLVGTPAIWLYQPLMALLLAFCAAPLYYLA
                     RTASMGRPAAAVAAVVASAPALVYAYAQMGAIKELAVLPFVLLLGPVLLLMPRWLERG
                     PRGAVLPALVGAAGVGAIGLAFLPWLGMTLLVAGLLLVIGSRSRRVDLRSLGVWLAAF
                     AVALVVFALPTFGALGDSLTLAKSLSTSNAVAVSDPGNLVRPLQRAQLAGIWIGGSHR
                     VDPAQHFALTYALIGIMFAAAALGIASLVRRRSWALAGFVVVMATVWVALTLRGTAWT
                     DAKLLVITSPIVVLLAALGADSLRRSGRRIEAVALAVPLVLGVLWSNALAYHETNLAP
                     TERLEELLSIGEERAAQGPTLVPDFDEFALYALADMAPAGPGFSSRPVSLATLRDGSV
                     TGYGHSYDLDSLDPEAVQGYATIVARRRPDASRPPANFRLAERGDYYDVWRRVRDLEV
                     TAHVPAGEGLQAAGFVPCERLAALGRRARASDASLWYSPRPLVSAFEGQSIRRRPAGW
                     GETVDGIGLATPGIAHKRFSVLASGVYDVWFKGDFARRLDVTIDGRRIGSIAGQSGNE
                     GNYARPLRARLTAGLHMLTLERPGGSLKPGDAAPTVLRAVVFAPADAGVPVARQIGPD
                     GWRRLCDRPVDWVEVARRR"
     gene            complement(23626..23883)
                     /locus_tag="VHF88_00685"
     CDS             complement(23626..23883)
                     /locus_tag="VHF88_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160322.1"
                     /translation="MELAVATVLFPLLLALLALGGGLLIDRIVGDVLPGILLPVVGLA
                     ALVVVAELCTYGEATASWVPYAFVLVGVGGLIAGRSRLRAA"
     gene            23951..24940
                     /gene="gmd"
                     /locus_tag="VHF88_00690"
     CDS             23951..24940
                     /gene="gmd"
                     /locus_tag="VHF88_00690"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX2160323.1"
                     /translation="MTTKRALITGITGQDGSYLAELLLEKGYEVHGMVRRSSTETFQR
                     LQGCRDDLTLHTGDLLDQRSLVDVMRACEPDEIYNLAAMSFVAASWNQPVLTSEFSGV
                     GATRMLEAMREVAPEARFYQASSSEMFGQVLEVPQVETTPFYPRSPYGVAKVYGHFIT
                     VNYRESYDLFACSGILFNHESPRRGLEFVTRKVTHAAAAIKLGLQTELALGNLDAERD
                     WGYAKDYVEAMWLMLQQDEPEDFVIATNETHSVRELVDVAFDQVGLDPADHVRIDPKF
                     LRPAEVEHLIGNPAKAKQKLGWEPRTTFEELVRLMVDADLELLASGVPQKQAG"
     gene            24975..25799
                     /locus_tag="VHF88_00695"
     CDS             24975..25799
                     /locus_tag="VHF88_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2160324.1"
                     /translation="MKLRELRWRAAGHYSQFGPEWTSPLAGARGLEIGGPSAVFRGDG
                     MLPVYPQLAELDGVQVPHAHALWHGELEEGAYETGEPGLSGRLWLRDGTGLDHLPSES
                     YDAILSSHVLEHIANPLRALEEWIRVLRPGGAFLIVVPHKEGCADHRRPTTTLEHMID
                     DLRAGTGEDDMTHADEVLRLHDIARDPPARDPETLRQRVLDNARVRAVHHHVFTLPSA
                     LRVLDHVGLRLIRATARWPHDIYVLAERPRPGEAVDNSALLDRGAVAGSPFTTDRA"
     gene            25815..26705
                     /locus_tag="VHF88_00700"
     CDS             25815..26705
                     /locus_tag="VHF88_00700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2160325.1"
                     /translation="MSPLVSVAIPTFNRRATIARAVRSALAQTHANLEVVVSDDGSTD
                     GSAEVLQELAAGDERLRVVRHAANAGMVANMERARRLARGDYVMLLADDDWLDPRCVE
                     LALAALLRSPDHVAALGGVDYVGSVAHPVRAELRPLTAAHPSRRVRDYFAAVDGDHGN
                     TWLYALIRRDAFDALSPLRNVLGFDWLHVAELAFLGKIALVDEVLIHRALGGTSETTA
                     RNVAVAGLPRALARIPHLAIGGEVLADVGWRSPVYAPLGRPRRLLLAVLCAAGVPLRN
                     LRHVLFHLAPRRLQRRWHAR"
     gene            26702..27805
                     /locus_tag="VHF88_00705"
     CDS             26702..27805
                     /locus_tag="VHF88_00705"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX2160326.1"
                     /translation="MTRSAIGIDARAATEEGAGGGRFVRELVRALAKRDDDRRYVLYA
                     RRRWEEPLDERFRWREVSGRDPIWHVRTALAANRECGAFLSTNSYLTTWFLRVPSAAV
                     VFDLVAFDPALRPQRRSGLIERLTLPLAARRSAALIAISQATADDLVERFPTAAGKTT
                     VAPLGTAPTLGDEARELPRGVPEGGFILAVGTLEPRKNLPRLVAAYERLPRALQDAHP
                     LVVTGRLGWDAGETMASLRALGDRAVLTGFVPDAELGALYRHCNLFVYPSLGEGFGLP
                     VLEAMAAGAPVLTSSRSSLPEVGGDAAAYCDPTDTAAIAAALEQLLRDPPRREELSTA
                     GPRRAAQFSWDRTADVTVEVLERISREAPARRA"
     gene            complement(27774..29075)
                     /locus_tag="VHF88_00710"
     CDS             complement(27774..29075)
                     /locus_tag="VHF88_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160327.1"
                     /translation="MRALRLLIVCPDRLGERMSGMGIRYSEIARALAAGGVEVTLAGR
                     ELDGGAIPGVRRTTWSADRLGSLRQLLRSADAVLAPPLAPHVLGAVRRSGAPLAIDLY
                     DPESLEVLERFRLSSAPVRALHATTATDRLVDALRSGHFFVCASERQRDLWLGAMLAL
                     GLLTPAVYDRDPTLRSVLDVVPFGVPDEPPPRPAGDPIRATFPEVERGDRILLWNGGL
                     WSWLDAPLAIRALARVRAHGVPARLVFMGASSAGGAGEALAAARATVLDEGLPESAVL
                     FNDTWVDYAARGDWLASADAAISTHRDHLETRFAFRTRLLDCFWARLPPVVSSGDTLA
                     DEIAAEDLGAVAAPGDLDTLASGIERVLANGRPYYEPALRLAAERHAWSRAVAPLRGF
                     LAAADQRPPRLARRNPLDVSPLRGGRRALQRVTRAVRALRG"
     gene            complement(29086..30588)
                     /locus_tag="VHF88_00715"
     CDS             complement(29086..30588)
                     /locus_tag="VHF88_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX2160328.1"
                     /translation="MSLIESAPAADRPGAEDIVGTAEAGPAALRGGLLRTGGFVLGLL
                     LGLASAPLIVRHLGDAGFGRYSAVLAVVAIVAGLTEGGVNTVALRELAAITDRAERDR
                     AMRDLLGLRLLLSCGGILLAVGFVVVAGYGVSLTEGALLAGLGMLFAVTQSLLATVLQ
                     SRLRFGWMTLIELIRQVLITGLIVALVLLDAGVVSFLAASIPGGIVALAMTVALVKDE
                     TTLRPSFNPRHWGALLRETVIFALAVAVNTLYLRVTLVIMTLLATAEQTGYFAISFRV
                     MEVLVTVPLLLIGSAFPIVARAARSDKERFAFAAGRMFELSVYVGALGALCLMLLAPF
                     AVEVLTGSSEHPSVVVLQIQSLAIAPSFVAVAATYMLLGMRRHRETLFANCLSLALAV
                     ILAIVLIPPFEADGAAAAAVIAELALAISSTWLLVRANGPGMPYSSLAVALVAAACGY
                     SAGAFADFHPLVDSAIAGMVFLVVLRVLGRFPPEVRDILRGLRSSGAPVG"
     gene            30668..31513
                     /locus_tag="VHF88_00720"
     CDS             30668..31513
                     /locus_tag="VHF88_00720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2160329.1"
                     /translation="MTAAPTCWLCDAPTSPCAALAPLPFRSCERCGFVFRPDLSETAR
                     AIYEDSAYEGTHGEIYTSPEYMRARDSDAAVRLRFLRGHAQGGDLLDVGASAGNFTAA
                     AARAGFRARGVEPTPGFARVARELLGTDVATGTIEDLDLPERSLDVVTMWHVLEHIPH
                     PTRELRRIRAALRPSGVLAIEVPNAGGVAARTDGTAWGSLEPDVHVNQFAPETLRLAL
                     ERAGFAIAVIETVPITPYLPPRRRLAPRHLAARAKAALRAGGLRGRHPHAHELLRAVA
                     TPAER"
     gene            complement(31510..32580)
                     /locus_tag="VHF88_00725"
     CDS             complement(31510..32580)
                     /locus_tag="VHF88_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX2160330.1"
                     /translation="MEVYARELIPRLAAVEGLRLTAFVNREAAAGEPGPWHAIPHETV
                     PVDARSRFQWVWGEQRHLPRMAARAGCDLVHSLASTAPVWGRFKRVTTVHDLNYKMVP
                     EAHFGLLRLGMGVLVPAAARRSQRVIVDAASTAVDVERHLGVPRAKIDVVPLGVGQQP
                     AVAPTPEPELRAKLDLGDRPILLTASAKRPHKNLMRLLEAHAKIGAAERPMLVLPGYS
                     TPHEADLIGRAAYLGTSDQVRFPDWVDQADLEGLYAAATAFVFPSLYEGFGLPVLEAM
                     RRGVPVACSNRSSLPEVAGDAALTFDPEDGAAIGAAIVRLLKDEPLRARLRRDGPAQA
                     GRFDWEATASATLAVYERALAG"
     gene            complement(32647..33843)
                     /locus_tag="VHF88_00730"
     CDS             complement(32647..33843)
                     /locus_tag="VHF88_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2160331.1"
                     /translation="MSTIETTPRPAGGDSQSGRASVSVVIPCLNEAENIETCVERALA
                     VLAEHDIPGEVVVADNASEDGSPELAAKAGARVIHEPRRGYGSAYLAGLAAATGDYVV
                     MLDADLTYDFEDIPAFVAKLDEGAQMVIGDRMDNIHPGAMPWLHRYVGNPVLTGILNT
                     FFRTGVKDAHCGMRALRRDVLPTLDLRTTGMEFASEMVIRASKRDLDIRQIPIEYHPR
                     GGESKLSSFRDGWRHLRFLLVHSPTWLFVIPGTAMAALGTLVALTVLADINLFGREWD
                     VHALIAGALLMIVGTQVLALGLCAHAYGTYFMGDQDPWFDRMRRRFRLEHGLLLGGGI
                     LLGGFAIAAVIAVTWIDRGFGELSETRLAVMAATLMIVGIQIVFSSFLLSILGLRRQD
                     SARAAG"
     gene            33868..35022
                     /locus_tag="VHF88_00735"
     CDS             33868..35022
                     /locus_tag="VHF88_00735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2160332.1"
                     /translation="MRVCVVYDCLFPHTVGGAERWYRELALQLAADGHAVTYLTRKQW
                     DDGDSPQLPGVRVIPVSPGGPLYTADGRRTIGPPLRFGLGVLRHLLRNRRSYDAVHLC
                     SFPYFSLLAARLALAGTPARIGVDWFEVWTLDYWRGYLGRIGGRIGWLVQRLCARLTP
                     QAFVFSRVHAERLLGEGLRRPALRLAGLYRGPLEPAAAGGERAPLVVFAGRHIPEKRA
                     EVVPAAVAAARKQVPGLRGLVLGDGPERERVLAAIAAAGVEDVVEAPGFVSGEDVSEA
                     FARATCMLLPSSREGYGMVVIEAAALGTPSVVVAGADNAAAELIEEDVNGFVAPATEP
                     RALATAIVACHAGGAELRERTAAWFARRAPELTVDASAHAVARWYATAAR"
     gene            complement(35019..35723)
                     /locus_tag="VHF88_00740"
     CDS             complement(35019..35723)
                     /locus_tag="VHF88_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX2160333.1"
                     /translation="MSATPAIAGEVSPSCRVDPFAVVGDGARIGEECVLHSHSVVGPG
                     ATLGARVEVFPGAVVGREPKGAGATARAPSFARELVVGDDCSIGVGAAIYYDVQIGPG
                     TLVGDGASIREGARIGARCIVSRCVTLNYEVTIGDDVKIMDNTHVTGRTTIGDGAFVS
                     TMVAMANDNTPTEELSGDTRLAGPTIQPGAVIGAGAILLPGIVVGAEAVVGAGAVVTR
                     DVEAGTTVMGIPARAR"
     gene            complement(35788..36750)
                     /locus_tag="VHF88_00745"
     CDS             complement(35788..36750)
                     /locus_tag="VHF88_00745"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX2160334.1"
                     /translation="MADGVTGKRVMLTGGSGFLGAQVRERLIAAGAAEVFVPRRADYD
                     LTDAAAVARAYEDGRPETVVHLAAEVGGIGANRDNPGRFFYANMAMGLHLIEEARHRG
                     AEKFVQVGTVCSYPKHTPVPFVEDDLWNGFPEETNAPYGIAKKALLVMLQAYRQQYGL
                     QSAYLMPVNLYGPGDDFDLESSHVIPALIRKCEEARVAGRGEVVCWGTGSASREFLYV
                     DDCAEAIVAAARRHDGPDPINIGAAREITIRELTETIARLTGFEGELVWDTSKPDGQP
                     RRSLDTSRARELLGFTASTDFEDGLRRTIEWYREHVAAAASAPS"
     gene            complement(36763..38265)
                     /locus_tag="VHF88_00750"
     CDS             complement(36763..38265)
                     /locus_tag="VHF88_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX2160335.1"
                     /translation="MSDARRLATGSLAQQAAQVTGLVAMFAVITVLARRLSLAELGVY
                     GLLNALAGYLLIVQNAAAGAAVRTMAAAADARAGSAAFSTAALLYVVAGALSGLLLAV
                     VGIGLAEALDLPDEVRRQARLGALAIGLVTLAGWPLTVYRDALRARGRFVLAAGTEIV
                     ALLAYVALVLGLALADAPLWSIVGAGAAIPLLAGLGCALAAWAARLPWRLRPRSASRA
                     VGREMLGLAGYISLSEAASAAVYALSRLILGVFKSAAAVGLYEGPVRAHNLIRALNAA
                     VTVTVLPTAAGYQASGDDRRQRELLVRGSRYTLALIVPIAVTGMALAGPILDVWLGSA
                     FREAGSAMAILMSHWVFNGMSGVAAAMLVAVGAARDLARWAVAVAAASVLLALALTPP
                     LGLDGVALATAIPYVALFPYLIRITLRAVPVAPAELLRRAFAPAWALGALLAATLVAA
                     RLTLDPQSALAVGALALGGLLAYWAAFFLVWLDRGERELVRAILTRPPRR"
     gene            38284..39111
                     /locus_tag="VHF88_00755"
     CDS             38284..39111
                     /locus_tag="VHF88_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX2160336.1"
                     /translation="MSRTPSAETVPPRAGAAAGVRRRDVLGVPIALVDYARALDVIDA
                     MVARRERGWICAAPAMSLVTAQNDPLLMAALRGATLTLPDGMPVVWAANLLGRRSGGE
                     RLDDRVYGPDLMRYHCQRAARNGQRVWLYGGHDDDALAALEASLLRDHPGLRIVGSWS
                     PPHSAPTAAEDCEIAERIDADAPDVVWVGLGMPKQERWMARLRPLLEAPVLCGVGAAF
                     DFHAGRVAQAPRWMQERGLEWAHRVVQQPRRQLPRYLRSNPRFVVGVARQYLRERRP"
     gene            39108..39998
                     /locus_tag="VHF88_00760"
     CDS             39108..39998
                     /locus_tag="VHF88_00760"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX2160337.1"
                     /translation="MTPALVTGGGGFAGRHLVELLADGVEAPPSSELDLCDAVAVRAR
                     VADLQPDAVFHLAAFSSPSESWQRPDQGLLTNLQMTLNLLEAVRHEAPRAAVVLVGSG
                     QVYGDPERLPIDEDAALAPGNPYAVGKAASDMLGRQYAEAFELRIVRLRPFNHAGPGQ
                     RDEYVLSSLARQVAAAEAAGATEMTLRTGNPESRRDFTDVRDVVRAYALASALDGGAF
                     NVCSGRSASVAELVALVAGHAQIPVRHEVDPALLRDHDARDMCGSHIRLTEATGWQPE
                     IPLERTVRDTLDWWRERLSR"
     gene            complement(39988..41202)
                     /locus_tag="VHF88_00765"
     CDS             complement(39988..41202)
                     /locus_tag="VHF88_00765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2160338.1"
                     /translation="MSAGPRVLVVHNRYRIAGGEERSVDLQLRALEAAGVEHASFERR
                     SADVGRAGAARALLRCGADPQELADAVRRLAGGGPALVHAHNTVPLVGPAGLAAARDA
                     GAGVVLHLHNVRIFCSTGFGERDGGPCRRCRGRNTLPGLVLNCRRSLPEAVAYTAALS
                     LHQPRLLDAVDRFVAPSAAARDELAARGLPAERLEVLRHYLPADSFAERSAAGDGGYA
                     FVASRLSPEKGIEDALTACLTARVPLRIAGEGPERERLERLAREAGATVAAAARASDA
                     PTSPPAAADVAFLGRLSAADVRRELGGAGMVLMPSRYHEFSPYSALEAMAAGVPVVAT
                     AMGGLPELLGEDRCVAIGDIPSFAARVAALWADPVGRAAEGDALIARARERHSRERYT
                     EDLLGLYAGLSG"
     gene            41224..42135
                     /locus_tag="VHF88_00770"
     CDS             41224..42135
                     /locus_tag="VHF88_00770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2160339.1"
                     /translation="MREPAVSVVVPTRNRAGYLEVTLASLAAQAPSVPYEVIVVDDGS
                     SDATAEVVAGAASLAQAPDVGSARPGDANPTSGPRVAYLRHKTSRGPNAARNAGIAAA
                     RGALIALIDDDVRVPPDWVGELAAGAERHRWAEALGGPIRASLEGPAPRSCGRESAPV
                     TTLDLGPRDCETDFVWSANMAVRRGAIERIGGFDEQVPIYGDEEEWLIRLHANGGRVA
                     YVAGAWLEHRRAGDDARLRSLARAEYRRGRAARRNDRRKGTAQPLHGELRDLAGAGWH
                     TARRRCAQGLIMGAHSTGRLVEALRGR"
     gene            42132..43001
                     /locus_tag="VHF88_00775"
     CDS             42132..43001
                     /locus_tag="VHF88_00775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2160340.1"
                     /translation="MSEALPDPASFLSGESGTVAGKKLTVTRTAADWTCNLAGLAALT
                     GPRLDRLAAVQTPRRVLVLSIYRPGSLLATAAARLGSDRHDVTFAFGATAEPDPALAS
                     DTVLDGLAGGKFENLNRLLAASPPLDDFDWLIAADDDVELAPRFLDRALALCERLGLD
                     IAQPAQTMRSHAAWRVTRRRPFSLARRTDYVEIGPVTLFSRRAAAELTPFPRLRFGWG
                     LDNHWGALARERGWRLGILDALPVRHESQKVATTYTHAEAIDEGRRFLAGRPYVTAAE
                     AQRTLATIRRIPS"
     gene            42998..44122
                     /locus_tag="VHF88_00780"
     CDS             42998..44122
                     /locus_tag="VHF88_00780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2160341.1"
                     /translation="MSARRLRILVVPKWYPWPDRPVFGAFCREHARALATRHDVVVLA
                     SLATPRPGFAAFQLEDAVEDGVRTIRVRYRRPFLRPAAMGFQLVGMLAALRRLRRDGW
                     RPDVVHAHVFEAAPPALALGRLSRAPVAITEHYTGFQRGLVTGYDRLLAKWSFERADL
                     VAPVSEQLAGDLRALAPRARFEVVPNVVDTSAFAPPAAAGERGAPRLLNVAALAEKKG
                     HRYLLDALVDLPDATLDIVGDGELRGDLERQANALALGDRVRFHGERSKEEVAALMRA
                     ADLFVLPSLAENLPVVLIEAQASGLPAVATSIGGVPELVDDQAGLLVPPADPAALAAA
                     IRAASERDFDPLALATRARDRYGYDAICARWTAIYERLLA"
     gene            complement(44181..45239)
                     /locus_tag="VHF88_00785"
     CDS             complement(44181..45239)
                     /locus_tag="VHF88_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2160342.1"
                     /translation="MAAVDIAIVSLGTTMGWRRGDEALATQIEAAGATCELRPMRLGL
                     ARHLQRSMATTDVLQALAARRAASGVDARAVVYSSITAALLQPLRRPHAVRFDTIAAL
                     SRPGVGGAWQRRRERAVLGRADLLLPWSEAAAEAALRAVAAGPAPSPPDVVVLPCVVD
                     LPGGVAADAPDATAYAANPEKRGLDVLCEAWRAAAPEGARLVVGGIGHEDALRWLARR
                     GVAEPTDVEFVGAVPRERWTALVAGSRVYLSAARYEDWGVAQMEALAAGTPLVTVPTP
                     GPNAALPIARRIGSELIASDASAPALAAALRTGLAMTDEQRARYGVAVRRALEPYSDA
                     ALRKIVAERVLPALLGSR"
     gene            45285..46388
                     /locus_tag="VHF88_00790"
     CDS             45285..46388
                     /locus_tag="VHF88_00790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2160343.1"
                     /translation="MSADPRPACLLTGIVSPYRREPFRLLAEAERVEVIAHEHAGPPI
                     PGLTVHRTSEIGAVRLAASGRYRAIVAGLDGRVALPGAYAAARARGVPFVLWATIWAH
                     PRTPAHALSYLPTRHLYRAADAVATYGPHVSRYVERYRGTDGGVVVAPQAVDVDHFGA
                     PVSDAERVAARERAGGPGTELLVLFVGRLVREKGVATLVAAWRRAGLAPDARLAFAGS
                     GPLDGRIRRAVPEARLLGQVAADELPALYAAADILVLPSIPTASFREPWGLVVNEAML
                     QRTPVIASDAVGAAAGGLVRDGRNGFVVAARDADALAARLRVLAGDAGVRERMGAAAR
                     EDAAAHTPAAWAAGMARALAAAGAGRADSGGDC"
     gene            46475..47044
                     /locus_tag="VHF88_00795"
     CDS             46475..47044
                     /locus_tag="VHF88_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160344.1"
                     /translation="MLPTALALIALLVPAQAASASVLDVQRDCADSDSFERKHSRADL
                     KKTLNQVQADLAEYGTCREMILAALASMKGGKGASSGKFAGVDPDLNNDGVITPKERR
                     IAAKKAKELREKRDRQIAAVDDDLIHDGDSGGVASGGGGSDGPPLPLILALVALACAG
                     AGGGLWYASQRNPAIANALRRVQLPFRNS"
     gene            47016..>47832
                     /locus_tag="VHF88_00800"
     CDS             47016..>47832
                     /locus_tag="VHF88_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160345.1"
                     /translation="MSSFRSATASAALAAALLGVALGARGGSALERTAIVELLLIAAG
                     ALAVCAAIAITPRGRIHGAAAVAAFALLAAITALSIEWSIAPELSYVEAGRTLAYFAT
                     FTGAVALARIAPDGTVAVARAIVLAAVAICGYGLATRVWPASFDELAFAGRIGQPFDY
                     WNALAGMAALGIVPALWLGARRDGSALGRALAFPATGVLVATLLIAQSRGALAGAAVG
                     CAVWLAIVPPLRGRPGCANAAARWPTRLRSRPRPRRARRATGRSAASRLGRRWL"
BASE COUNT         6842 a        17586 c        17068 g         6336 t
ORIGIN      
        1 ggccccaccc ccctccccgg gccgcgacga gaccccgctc gagcgcaccg accgcaacct
       61 ctccgagctg ctcggagagc tgcgggtcgc actgccgggg gtccaggtcc tgttcgcgtt
      121 cctgcttatc gtcccgttca acagccgctt cgacgacgcc acgcccttcc agcgcgacgt
      181 ctatctcgcc acgctgatct gcaccgcctt tgcgtcggcg ctactgatcg cgccgacgat
      241 gcaccaccgg ctcgagttcc gccacggcga caaggagcac ctcgtcaagc tctccaaccg
      301 gctggcgatc gccgggctga cgctcgtcgc gctcgcgatg accggcgtga cggtcctcgt
      361 gaccgacttc gtcttcgaca ccgtcgaggc ggtcgcgacg acgggcgccg tggcgctggg
      421 gttcgcgacg ctctggttcg caatgccgct gcagcggcgg cggacgctga gccggcggcg
      481 tgactccaag cccgcccagt cgaccgatga cggctagacg cagcgcacgc cggaccagtc
      541 gtgcgtctcg agcaccatgt actcgatgaa gtaggcgatg cagccgggcc cgtcgccgcc
      601 agggggcagc acgggcgcgc agtcgggatg gcgcgggtcg tcgccgtggg tggcggcgca
      661 ccaggcgtac caggccggcc ggcggctgaa gtcgccgtcg agcaggccgg tgtcccagtc
      721 gggaccgccg ccgcgcatgt tgtagtaggc gacgcgcttg acgcgcgggt ggttggcgag
      781 cacctcggcg atccattcca cgcgcgccgc ctgcgcggcg gaatcgccct cgtggtgggc
      841 gccgacctcg gtcacccaga tgtccttgtc gggcggtacg agcgccgcga acgcgtcggt
      901 gaacgtcgcc ggcgcggtcg gctcgaccgc gcgcttgacg tcgccgtagg ggtggagggc
      961 gacgacgtcg ccccagcccc cgccggccgc cgtcgagtag gtggtcacat actcggtgaa
     1021 ggaggcgagc gacccggcgt cgaggaagtc gggtgaggtg atcagcgcgc tggggtcgtg
     1081 gcgacggacg acctcctgga aggcgaggta gtagccggcg agcgtgagcg cgggcagcgg
     1141 cgcctggccc gccgggtcct ccttcggccg ccaggcgatg ttcggctcgt tggcggggcc
     1201 ccagacgtca acgaccgggg ctagctgctg gacgagcggc tcgaccttgg cgcggtactc
     1261 gtcggcggta gggaaatagc cctcggggcc gacgttggcc gggttgttgg aacgcagcgt
     1321 gaggatcacc tgctgtgcgc cctggaggcg agcctcggcg atcatcgcct gcgtgcgggt
     1381 catccactcg ggacggtcga cggtgttcca gatcgtctgc agccgaaaga ccctcggccc
     1441 tagcttcgcg aagtcgccgc ccagggcggg cagcgcgccg gggttagcga agtcccagtc
     1501 gtcgtcggcg acgaagaggc cccagccgtc gcgcgagttg gcggcggtgg cgggcgccgc
     1561 gccgcctagg acgaggccga ggcaggcgag gatcgggagc aggcgacgca cagcgcccga
     1621 ggttagtggc gatcaggcgc cggtgagacg gaacagcgcg aagtcgttgc caccgacgcc
     1681 gtcgagcatg ccggtgccgt cgaagagctg gagcacgagc tcgtgctcgt cagccagccg
     1741 ctcgcacgtc ggccgcaccg agggccatgc ggcatcgtcc atcaccacga agccgcccgg
     1801 ccgaaccttc ggcaggaaca gcgcgacgtc gcgctcgacc gcggcgcggt catggttgcc
     1861 gtcgacatgg agcaggtcga tgctcgcggg cgcaaagtcg tccgcggcat cctcggagcg
     1921 cttgcgcacg atccggcagt ggcagtcgat gccgagcgtc gcgattcgcg cctgaacgtc
     1981 gtcgtgtatc ccctgccagt ccacctgctg cgcccacgag gcgaggtcga tcgagcggcc
     2041 gtgcgaatcc gactggaccg ccgccgcggc cgagtacggg tcaacgccga tcgcctcgcc
     2101 gtctagcgcg cgcatcaaaa tgccgagcgg gagcagcaga cgcccgcggt agacgccgat
     2161 ctcgacgacg tggcgcaggc ggttggcgac gatcgcgtcg gcgatcgcga gcaccttgag
     2221 cgcgcttgcg cccccgccct cgtcgacgcg gatctcgccg ttgaggcggc tcatctcatc
     2281 gcgcagcgtg tctggcagca accgccccgg acgggcgtcg gcggtgtcgg cggaggccac
     2341 gccgccactg tacatatcgt cggtggcgtc tacgctctcg ctcgcatgag gatccccgga
     2401 ccgaccgcgg ccggcgaaag cgcgtcgtct ccgctccgtt tccagcgacc atccctgccg
     2461 agcggcgacg cgatcgagcg gtacctctcg ctcgcccgcg aggagcgctg gttctcgaac
     2521 ggcggaccgt gctggaagct gctgcgcgac cgcctgtccg agcgcgtcgg tgcctactgc
     2581 gtgcccgtcg ccagcgggac ggtcggcctg atggcagcgg tcgcggccgt cgccggggac
     2641 gccccacctc actcaaaggc gctactgcca tcgttcacct tcgcggccac ggtgcaggcg
     2701 gcgatctgga cagggctcga tccgttgctg ctcgacgtcg attccgccgg ctggcacatg
     2761 gactgcgcgc agctggagga ggagctgcac gccgccggcg cgacggccgc cctcgcgcta
     2821 gtggtctcct ccttcggcac gccaccgccg ccggctacgc gccagcgctg ggagcaggca
     2881 tgccgtgcag ccggggtccc gttggtcatc gactcggcgg ccgggttcgg cgcgatcgcc
     2941 gccgacggtg tgccgatcgg cgcgcagggt gatgtcgagg tcgtctcctt ccatgccacc
     3001 aagccgttcg cgatcggcga gggcggcgcg gtcttctcgc acgacgcacg gctgatcgag
     3061 cgtatcgagc gcacggtgaa cttcggcctc gaccgcgacc ggtcggtcgt cgacccgcgc
     3121 gggctcaacg gcaagatgag cgagctgcat gcggcgaccg ccctcgccgt cctcgacgag
     3181 ctcgactcga tcctcgcagc acggcgcgga gcggctgcgg aggtgcgcga gcgggccggc
     3241 gcggacgtct cctggcaggc cgagagcgaa cgctcggcgt ggcagttcgt accggtcgcg
     3301 ttcgccgacg cgcgggcacg cggcgaggcg caggagcgtt gtgcggcgag gatcgagaca
     3361 cgcgtctact acgagccgct gcaccacatg cccgcgtatc gcgagctgcc gcacgccagc
     3421 ggagggctgt cggtcaccga gcggctcgcg cagcggctgc tctgcctgcc gatggccaac
     3481 gacctcacga gctcggaggc cgtccggatc gcgaccgcga tccgcgggcg ggccgctcag
     3541 ccggccggct gagcgcggcg cagtgggcgc gccgggacgc cgaccactgt cgagccggcg
     3601 gccacgtcgg agacgaccgc cgcgccggcg ccgacgacag cgcctccgcc gacgcagacg
     3661 tcggggcgga tcaccgctcc cgagccgacc tccgactcgc actcgaccgt cacgcgcccc
     3721 gcgagtaccg cgccgggatc gatcacgacg gcgtcggcga gtcgcgcgtc gtgctcgacg
     3781 atcgccccgc tgttgattag gacgccgcgg ccgagcgaag ctcccgcgcc gacggtcgcg
     3841 ttcgggagca cctggcagcc agcggcgagc gtcgcgctcg ccgtcacgtg cgcgctcgga
     3901 tggatcagca cagggagcgc gaggccggcg gcctcgacgg cctcgaccac cttcgcgcgc
     3961 ccgcgcgcca cgccgaagcc caacacgacc gcctcgacgc cctccccaac cagccgcccg
     4021 agcgcaccgc gatccccgat gacctcgagc cccgcgacgc ggttgccgcg gttctccggc
     4081 cggtcgtcga gaagaccgac cagctcgagg ccgctgtcag cgccgaacaa ctcgacgacg
     4141 acgccagcgt ggccgtcgga ccgcgacccg tagacgacgg cacgcacggt cagcgcaccg
     4201 ccgctatgtc gctcgagaag cccatcgcgg cgagcttctg cgcggcgccg tgacgaacgt
     4261 ccatccgcga tcgcagccgc tcactgccgg cgctggcgac tcgccgcgcc catgccggat
     4321 cggcggcgag acgctcgagc gtcgcgcgga ccgcggcggg ccgcgctaca cgctcgaggt
     4381 agtggacctc cggctcgagc acgcggtggt cggccgcggg attggccgac acgagcaggc
     4441 agccgctgct catcgcgtca gcggccgcct gggtcggaaa gccgtcggtg acgccctggt
     4501 agctgcctgg cgggcccgcc gcctcggcct tcaccggtga gaggaagaca tggacctcgc
     4561 ggtgcagctc gcgcagctcg gccggcgata gccagccgtg gaaggtcgca aggccgggcg
     4621 gcagctcgtc gcgccgctgc tcgtgggggc cgacgacgtg aaggtggaac gcctcgggct
     4681 ccaggtcgcg gaaggcctcg agcgccacgt cgagcccttt gcgcggcggc gcgtcggcgg
     4741 cgaagaggca ggtcagcgga cgcggccgct cccaacgctc ggagctgacc acgtagaacc
     4801 gcgtctcggt gaacgcctgg tccacctcgg tgacgcctgg gaccgtatcg agcacctcgc
     4861 gcacgtacga gaacgtcgtg tcgagccgcg cggcgagctc gcgtacgcgg gccagcccgt
     4921 gtggggtcgg cgtgaagccg ccgcccgggt acacgctgcc atggagccgt atccccgacc
     4981 gctcgaagac ggcggacatg tcgaacggcc ggaagggatg gccagcctcc tcgtcggtcg
     5041 tcggttccat cccgcagagt ccctcgagga atatctggaa gaccccatag gcgtcggtga
     5101 tcccggcgcc tggagcgatc gtgggcagct cggccagcgg atgcactggc gccgggaacg
     5161 gatcggtcat ctcccacatc gagaagaaca gcgtgtccgg caggaggtcg tggatcgccg
     5221 ccgcctcgtg gtaacgaaac ccgctccgct gccaggggaa ggtggcgtca aggaaggcta
     5281 ggcgctggcg gcggccgcgg cgcaggcggc cgcgcgctct cccgagggcg gcggcggcgc
     5341 ggctcggctg cacaagccgc gggcgctcct gtccgcggtc gcgcgccggc aggccgtaga
     5401 agtcgacgag gtcggcgaac tgcacggccg cagactagtc gtcgcaccca cgccccaggg
     5461 ctgtcccgcc gcggtcgcgg cgagcggatc gaggctaggc tgcgccggca tgaccgatcc
     5521 cgaggggcag ctggatgcgc tcgcgcgccg gctcgaggag ctggcgagcg agctggccgc
     5581 cgtccgcgag ctcgccgcgc gcgcgtacga cgacacaccg gcgctcgccc ggcggctgtt
     5641 cgagcggcgt cggtcgcccg agtggcagga cgcatacgcc gccggcccac tggtcagcgt
     5701 ccggatcggc acctatcgcg gcggggacct gctgttcgac cggacgctcg ccagcgtgct
     5761 cgcgcagtcc tacgagcact gggaggcgat cgtcgtctgc gacggccaag agcccgagac
     5821 ggcggcgcgc attgcggcgc ttggcgatcc gcgcgtccgc tgctttcagc gcccccgacc
     5881 tgggccctat cccgaggagc agcgcgcccg ttggcatgta gcgggcagct tccctttcaa
     5941 cgaggcggtg gggcgtgcgc gcggcgcctg gatcgcgccg ctggaccatg acgacgagtg
     6001 gacggcggac catctggagc tgctgctgga gcaggcgcgg cggacccgcg ccgaggtcgt
     6061 gtacggcgcg tttcgcgcgc tcgtcgccga cgcgggcgag acgtggttcg gagcctggcc
     6121 gccgcgccag ggcgacttcg gtttccaggc cgcgatctac cacgccggcc tcgccgacgc
     6181 tctctacgac gccaacgcgc acctgctcga cgagccgggc gactggaacc tcgcccgccg
     6241 cctgctcgag gctggcgtac gttgggactt cgtcgatcgc gtcgtcggca cctactacgt
     6301 cgacgccgac gccgccaccc tcgactggtg gcgcgagcgc gtggccagcc gcggcgcgca
     6361 ccccgacagc gcggcaagcg acctgccgcc ggactagtct gcccgcgatg cccggcctgc
     6421 cccgcatctg catcgtctcc gcgagcggtc agaacgtctt cttcgccgag atcctcgagg
     6481 cattcggcga cgccctgcgc gaccacggag ctgtggtcga gcagtccgtc gactgcttcc
     6541 cgccggccgc cgaagacctg gtctgcctct acgtcccgca cgagtaccac ccactcgtgc
     6601 aggagcccgc tcatcccacg cccgtccagc tcggtcgctc ggtcgcgctc tgcaccgagc
     6661 agcccggaac ccagtggttc gagcttgcca cggacgtagc agcccgcgct ggaggggttg
     6721 tcgacatcaa cgcgctcggc gcgcgcgagc tcgagcggcg cggcatcgcg gtcgagcacg
     6781 cgccgctcgg ttacgtcccg agctgggacg cctggcatgg ccgcgagacg ggcgagcgat
     6841 cgatcgacct cgcgttcctc ggcagtcaca ccgatcgccg ggcgggagcg ctcgcacgtt
     6901 gcgcccccgt cctccgcggg cggcgcgccg ccatcaacat ggtcgagtcg gtgcagccgc
     6961 acatcgccgg cagcggttcg ttcctgtcgc acgaccgcaa gtggcggttg ctcgccgaca
     7021 ccaaggcgat cctcaacgtc catcggacac ccctcggcta catggagtgg caccggatcg
     7081 tcggagcggc gctcaacggc tgcgtggtgc tcagcgagca ctcgctcggc gtggagccct
     7141 tcgtccccgg tgagcactac atctcggcga gctacgacac gctgccagag gcgctcgagg
     7201 ggctgctgag tgataccgac ctgcaacggg ccacgcgcca ggcggcctac gagctgctgc
     7261 gcgagcggat gccgatgtcg acggccgtcg atgcgctgct ggcggcggcg gagcgcgccg
     7321 cgcgcggacc gctgcgaacc tcgggcgtcg cgtcggtgcc cggcgttccg atgccgaagc
     7381 cgccgccgcc gcgccagccc gactgggcgg cgttcgcaac ggaggtcggc gatcagctgc
     7441 cggtgcggat ggccctgaag cacctcgtcg tccacacgcg caatctggag cggcgaatcg
     7501 agaagctggc gagcggggac ggcgagcagc cagccgagga cgtggtcgag cggctcggtc
     7561 ccgacgtcga cgacgtgcgc gtcagcgtgc tgctgaccgt ccacaactac gcggactacg
     7621 tcggccaggc gctgcacagc gtcgcgctca gcgacctgcg cgagatcgag gtcgtcgcgg
     7681 tcgacgacgc gtccaccgac gactccgtcg acgcgatccg tacagcctgc gccgaagtgc
     7741 cctggctgcc cgtcacgctg gtgcgacgtg gccgcaacgg cgggctgccc gccgcacgga
     7801 acctggcgct cgcgcacgcg cgcgccgacc tactgttcgt cctcgacgcc gacaacgccg
     7861 tgctgcccga ggggatcggg cgactggcgc aggcgctcga cgacgatcgc gaggccgcgt
     7921 tcgcgtacgg catcatcgag gccttcagca caagcgggcc cgtcggcgtg atgaactggc
     7981 tcgactggga ccccgaccgt ctgcgctatg gcaactacat cgacgcgatg tcgatgatcc
     8041 ggcggtcgac gctggaggcc gtcggtgggt attcgaccga gccggcactc tacggctggg
     8101 aggatttcgc cctctggtgc gcgatagcga gcaacgggct ccgcggcgtt cgggtcccag
     8161 acttcgtggc gcgctaccgg gtcagcgggc actcgatgat ctcgctcacc aacatcgaca
     8221 gctcggccgc ctgggcgacg ctgctgcgca agtatccggc gctggcatag attgccgtcg
     8281 tgaccttcgt gcacgagcag gcgatcgtcg atacctcgag cctcggagag ggcagccgcg
     8341 tctgggcctt cgcccacatc ctcgagggag cgcgaatcgg acgcgacgca aacgtctgcg
     8401 accacgtgtt catcgagaac gacgtagtcc tcggcgatcg cgtgacggtc aagagcggcg
     8461 tgcagctatg ggacggggcg cgcgtcgagg acgacgtgtt catcgggccg aacgtctcgt
     8521 tcgtcaacga ccgatttccg cgcagcaagc aacaccctga tcgcttcctg accaccaccc
     8581 tgcgcgatgg ctgctcgatc ggggccggag ccacgatcct cggcggcgtg accgtcggta
     8641 cgggcgcgat ggtcggcgcc ggcgccgtgg tgaccaggga cgtgccgccg ttcgccatcg
     8701 tgactgggaa cccggctcgc atccgcggct acgcgggcgc cgatcgcgcc ccattcgagg
     8761 atgccgaccc tgggagcgct tcgcctgagc cgggagcaga tgtgggtggg gtccggctga
     8821 tcagcctgca ctcggtgacg gacctgcgcg ggagcctctc ggtcggggag gtgggcgccc
     8881 agcttccgtt cgtcccgaag cgcatcttct tcgtatacga cgtaccgacc gccgaggtgc
     8941 gcggcgagca cgcacaccgc cggttggagc aggtcctgat ctgcgtgcgc ggcactatct
     9001 cggtcgtggt ggacgatggg cgggcgcgcg cggagtacaa gctcgaccgg ctcgatcgcg
     9061 cactgcacat acccccgaag gtctgggcga tccagtacgc ctactcgcgc gacgccgtcc
     9121 tgctggtgct ggcatccgag gagtacgact cggacgagta catccgcgac tacggcgagt
     9181 tccggcagat caccgcgagc tgaccgaagg gtcgacgacc tccgagacga tcgccccggg
     9241 ccggcccttg accgcctcgt atgcgcgata cacgtaggag ccgatgatgc cgagcccgag
     9301 acagttgagg ccggcgaaga agatcacgac caggatcgtc ggcgcatagc cgggcacgtc
     9361 gatcgcaccc gtcagtcgca gcactgccac cagcgccgct atcaccgtcc cgagcgccag
     9421 cccgaccagg ccagccgccc acagcatgcg aacgggcaga tcggtgaagg cgaacacgct
     9481 gtcggagagg taacgcagct tcttgcgcag cgtccagccg ctcttgccgg ggcgggggcg
     9541 ccggtgatag gggatcagct cgcgccgaaa gccgacccag aacagctgcg cgacgagcga
     9601 ggtgttctgg tcctcgagcg aggtcaggac gtcgcgaacc tgtgccgtgc aggcgaacac
     9661 gtcgaccccg tccggcggta tgtccgccat cacgaagcgc cggtacaggc gccagtagag
     9721 gctcgaggcg ccgtcgccgc gatcgtcccg gctggcgcgc tcgccggcga cgatgtccac
     9781 ctccccgctg gccagccgcc tgaagaactc gatcaccagc tccggcggct cctgcaggtc
     9841 ggccgccatc accgcgatgt accggccgtc ggcgatcgcc atgccggtac gcagggcggc
     9901 gaacgacccg aagtttcgcg cgtgcgacac gaggcgccac ctgaacggcg cggcgtcgag
     9961 cgccgcgagt agccgctcac gcgagtcgtc ggggctgccg tcgatgacga acacggcctc
    10021 gaagccgtcc accacctgct cggcgatgtc accgacggcc gtcacgagcg cgtcgatcat
    10081 tgcggcgttg cgatagatcg gcacgaccag cgagttcgcc ggagcactca cgcggccagg
    10141 ctcccgcagg catcgatcac gcgctcgacg tccgcgtcgg cgaggtgggg atgcagcggc
    10201 agcgacagct cgcgctcggc cagacggcgt gccgtgggaa gcgctgtggc cgtcgatccg
    10261 gttcccgcgc aggcgggttg gtcggggcag aggaccggat agtggcgccc gaccgacacg
    10321 ccggcggcct cgagcgcctc cgcatgccgc tcggcctcgc cctcgatcac ctcgaccggg
    10381 aacaggtggt tcgccgatgt tccgccggtg ggacgcaccg cgctcaggac aggcgagtcc
    10441 tgcaacgcct cgacgtagcg ctcggcgacc tcggctcggc gacgcagcca gccgtcgagg
    10501 cggggcagca gcgccgagcg caggaccgcg gcgtgcagct cgtcgagcct gctgttcaag
    10561 ccgggctcga cgtggcggta gcgctcggac tggccgtagt tgcgcaagcg ccgcgcccgc
    10621 tctgcgagct cggcgtcgtc ggtcagcagg acgccgccgt cgcccatcgc acccaggttc
    10681 ttggtcgggt agaggctcgt ggccgctgcc acgccggcgc gcccggtggg cgctccggca
    10741 cgggccgcgc ccgcgctctg cgcgcaatcc tcgaccacga cgaccgcgtg acgctcggcc
    10801 atggcgcgca gcgcggccgg atcgagcggg tgcccataga gatggacggg cacgacagcc
    10861 cggatcgacg gatcggccgc cagcacctcg tcggccgccg cgaggtccat cgatcccgac
    10921 tcgtcgacgt ccgaccagat cggcacggcg ccggctctca tgatcgccaa ggtggtcgcg
    10981 aacgccgtca acggcgtggt cagcacccgg tcgcccggac cgatcccaag gcaacgcagg
    11041 gctatctcga tcgcatcgag tccgctcgcc accccgaccg cgtgctcgac gccccaccag
    11101 gcgcccagct cgcgctcgaa cgcctcgacc tcggcaccca gcacgagcca accgctgctg
    11161 ccgacccgat cgatcgccgc aagcgcgtcc tcgcgaacct catcccactg agccgcgaag
    11221 tcgttcgcga ggatcgtgtc cggacggacc gtgctcatcg agccgtctcg gcccccgatt
    11281 gcgcgaacga gaagaacttg ctgccgagga aaccaacggg agtggcgatg acgacggcga
    11341 gggcctgagc gggcaccgcc gccagcccgc cgactccgtg cagcagatac agcgccacgt
    11401 agttcacgat cagagacgcc gccgccacga cggtgaagcg acccagctcc cggtggacgg
    11461 cccggccgct gcgcgggaac gaccaatggc ggttgagagt gaagttgaac aggccgttcg
    11521 cgacgaagac gggtggcacg gcggcgtagg ccgacacccc gagcgcgatc tcgccggcat
    11581 agagcgccat cgccaacaca tacccgcagc cgccgaccac gacgaaacgc agcatctcct
    11641 cccggtggtc gcgaggcggg cgcaggacgt ttcgctggag ccagtcgcgc attcgtggct
    11701 ccagaccgta tcgagccctc acggacgtca gcgtcatacg aagcgcagtt gctccgtagt
    11761 gcttccgacg acgtggagga ctaccgccat atacagagcg aacgcaaccg ccaggccagc
    11821 caccgtgaca gaccgctgac gagcggtggc gcgcggcgcg agcgggtttg gccagccggg
    11881 ggcgagggcg agcaggtagg cgagcacgta cgccgggagc aggaagcgcg tctcgaccgc
    11941 ggacggcagg gacgccgcgc cgcacaggag caggccgacc gggtagcgcc accgggatgg
    12001 tccgaggctg cgtcgcgccg ccggccaggc cacgcgagcc aatgcgagga agacgagcag
    12061 gaagccgagc acgcgcatcc agcgctggga gcctgtgtcg acctgctcaa tgtatggagt
    12121 ctcgtagcgc tgatcgaaac cgtttatgac atgccgcgcg aagaggcccg ccatatgatc
    12181 ggatggctga cgacgatgcg ggcgtactcc ccggcgtcgt cgacgacggc gtcgtcgcgg
    12241 ctctgaagga tccgctggcc ggtcggatcg aagtacatca tccggccgcc cggacggtcg
    12301 ctgccgacga aggtctcgta gcgctgcagt gtcagcccgg cggtgaactg gaggctcgac
    12361 aggtcggcga cggcaccggg cacgaagctc gagctgtcgt gatggcggtc ggtcgccagc
    12421 gactggggca acgagatcgc cgcaaatccg acgagcagcg cggccacgcc gagcaacccc
    12481 cgggcggttg aacgacgtgg cgcggcgcgc gccttccacc aggcaatcgc gaggagcccg
    12541 acaacggccg ggactacgag gatgtacgcc ggccgcatgt tgatcgccgc cgcgctcgag
    12601 gctcccgcta caagcatcca gccgggcgcc tcggcgcgcc ccaccgcgac cagcgcgagc
    12661 atcacggcag ctagggcggg gaagtcgctg agcggaaagc tcatgtacga gcgccagaag
    12721 acgagcacca gcgcggccag cccgagccgg cgccagaacc cccaagtcag ctccggccat
    12781 gcgaggcggg cgaggctggg cgccagaacg gtcgagatcg ccgcgacgat cgcggcgttg
    12841 aagatcttca cgaccgacga cgggctccag gtcagcccgt ccgcgactcc ggctaggccg
    12901 tggtagatca gcgccaggag gtagccgcgc agcgggctgt cgaagttcag cagcgagaag
    12961 tcgccgtcga cgacgaagct gtctccaagc tcccagtagt gcccggcgtc gtagtagaac
    13021 ggcttctcgc catggagcag ggcgatcgtc aacgcggcgg caaaggccac gccgaaggcc
    13081 gcgacggcgg gcgttcgcag gtacatcgag gcgggcacga tatcgatggc cgccactaca
    13141 ctcacccgtt tgccgacgcg caccccccca tcgcgccccc acacggccgc ttctcggccc
    13201 gtcgcaccgc cgctggcgcg ccttcgcgcc cgcgctggcg actcgaagca cctgccggtc
    13261 gcccagacgg cgatcgtcgc gctgctgctc gggctgatcg ccacggtcgt ctacgggccg
    13321 cacgcgatcc acggcggctt tctgtcggac gcttgggcgg cgcgaacgct gtacgagttc
    13381 gcgccgcagg acgacttcct cggccgcctg ggacacctgc tgagtcagcc gaacgtgagc
    13441 ccgcgaccgt tgcagggcgt ctacgtcgcg gttcagaacg ccgtgttcgg gccgcacatg
    13501 gggctttggc tgacctggca gggggcgatg agcgtggtga tgtcgctggt cctgtaccta
    13561 ctgctgcgtg agctgcggtt cagttggcag gacgcgggag caattgcggt gctggtgctg
    13621 atcttcccgg ccgcgagctc gatcaggctc tggacggcgc cgatgagcgc tctgacgatc
    13681 tcgctggcgc tgctcggctt cctgctggcg ctccgggcct tccgccgcga cggcgctcgg
    13741 cgcatcgcgc tgcacgggct gtcgctggct gtgttcgccg ccagcctgct gctctacgag
    13801 gtggcgctct cggcgatgct ggcgtccgtg ctggtgtacc ggctgtgcgt gccatggcgt
    13861 gcagcagggg cgcgctggct cgtcgacgtc gtggtgctcg tcccgctcgc ggccctggtc
    13921 actcgctcgg agtcgtcctc gtgggaggcc cagaccttcg acgggatgtg gcgccacgcg
    13981 accgtcttct gggatgaggc gcagacgctg ctcacctcgg tggtgctgcc cttcgggacc
    14041 gaccgctggt acgccctggc tctgatagcg ctgatcccgg cgatcgcggt gctcctggca
    14101 cgccagctcc cgtcgtccga tccagcccgc gccgatctgc gtcgctggct cttgaccgtc
    14161 ctggccggac tggcgatcgt cgcgctcggc tacgcgatgt ttgtgcccgc gatggactac
    14221 tacacgccgc tccgacccgg gatcgcgaac cgcatcaacg ccgttcccag catcgggtgg
    14281 gtgctcgtcc tctacggctt ggcgatgctc gccgccacga tcgcgttccg cggtcagcgc
    14341 caacggcgac tcggggcatc tgcgcttgcg ctggtcgcct gcgccatagt cgccgccagt
    14401 tggctggaga gcatcgacag ggaggccgag gcatacatgc gcgcgtactc ggagggtgag
    14461 cgcgttctgc aaaccgtgga gacactgctg cccgaccctc caccgcacag cgtgatctgg
    14521 accttcggtc agcccgtcga gatcgcgccg ggcattccgg tgttcggcaa cacgtgggac
    14581 atgaccggca gcgttcagct tatgtacgac gacccgacga tcaccagcta cgtcgggcat
    14641 ccgggaacga ccttcgactg cgagcgcgag cgaatcgtcg ccggcgggcc ggcctacctg
    14701 gtcccgagct cgccgggtgc ccagccgctg tcgtcgccgt acggcccgac ctacttcgtc
    14761 gacaccgtcg acggccgctt gcagcggatt cgcagccgtg cagagtgtcg ccgcgcacgg
    14821 gtctcgttcg agctgtcgcc ggcgctcgcg ccggcctgac cgctacctca agccgcggct
    14881 gaggcggcgc ttgattcgcc gcaccggctc ggtcatgcgc caggtcagcg agccctcgag
    14941 ggcattcagt cgcgaaagca gctgccggtt ctcctcgagc tgctcctgca cccggctctg
    15001 catgagcggg atctgcgcga gcgcctgctc ggcgtccagc agggcgcggc tcgtgtcctg
    15061 aagccgctcc gtggccgact gctcagcggc agtcgcgcgc gcgacgctgc ggtgtgcctc
    15121 ggcttcaacg ctctggacgt gatcggccca ccaactgacc tcgaacgcgt ccccgagcgc
    15181 gaccacgctc tcgagcgggg gcagcggagc atcgctggcc gcgacgatgc cgtacgtctc
    15241 gccgccgccg tcgagtgccg tcgtggctct tacgtgcgcg gagatatccc cgtcgggtcc
    15301 gccgtgaccg ttcgcgcgct cgatccgcgt cgccagccac gggtgctggc gatacgcgtc
    15361 gacgttggca aacagctcgc cgactgccgc ggcgagctcg tcgggccggt actcgtggac
    15421 gtggtgctcg ttgccggacg ggtagacgtc cggattcggt gacgagacga gcagcacgcc
    15481 gcccggccgc agtacgcggt gaagctcgcc gagcgttcgc gccccgtcgg ccacgtgctc
    15541 gatcgcctcg aagcagacaa cgacatcgaa ggacgcgtcg ccgaacggca ggtcgagcag
    15601 gtcgccacgg gtcacggcgc tgggatcgcc aaaccgttcg cctgcctcag ccaccgcatc
    15661 gggatcaatg tcgacgcccg acacgctggc cgccccgccg ctcgcaagaa gcgccatccc
    15721 gtagccggtc ccgcagccgg cgtccaagac gtccttgtcg cggactgcct gcgcggccca
    15781 ccagtagcgg ccgcggtgct cagtgtcgat cagcgtgccc tgctggtccc gagggtcgaa
    15841 gcgctcggga tgtaccgcct gctccattga cgcgatcgta ctgagtcggg cctgccgcgc
    15901 ggatgccgct ggtaccgttc gcgcacttgt ccgccaccgt ccaacagccg gcggcctcag
    15961 gcatgctgag cgcggcgact gagcctccgg ccgtctccgt cggctccgtc tcgaagagct
    16021 tccggctccc ccacgagcag tactcgacgc tcaagcagcg cgtgctgcac ccgcgccgct
    16081 cgcgcacgtt cagcgagttg cacgcgctag aggacgtcag cttcgagatc gcacagggcg
    16141 agttcttcgg catcgtcggt cgcaacggca gcggcaagag cacgctgttg aagtgcctcg
    16201 ccgggatcta cggggtcgac agcgggcgga tcgacgtgcg tggtcgcctg tcgcccttca
    16261 tcgagctggg cgtcggcttc catcccgagc tctcggcgcg tgacaacgtc gtgatcaacg
    16321 cgatcatgct cgggctgacg cgcaagcagg cgctcgaccg cttcgacgag atcatcgcct
    16381 tcgccgagct cgaggagttc gtcgacctca agctgaagaa ctactcgtcg gggatgagcg
    16441 tgcggctcgg cttcgcggtc gcgatccagg tcgacgccga cgtcctgctc gtcgacgagg
    16501 tgctcgcggt cggcgacgcg tcgttccagc agaagtgctt cgaccagttc gaccggctga
    16561 aggccgaggg gcggacgatc gtcttcgtca ctcacgacat ggactccgtg cggcgcttct
    16621 gcgaccgggc gatgctgctc gagcgcggca acccggtcgc aatcggcgag ccggacggga
    16681 tcgcggccaa gtacgcggag atcaacttcg gcgtcgtcgg cgaggacggt gcgggcggcg
    16741 gcacgggact cccccgagcg cgaatcgcgc gcgcctggtg cgagacagca gacggatccg
    16801 ccgcggagac ggcgcgcgac accttcaccg ccggacgtgg cgagccctgc gctgcctgca
    16861 tggagatcga gttcagcgat cccgtcgagg accccgtctt cacgatcgcc ttccgcaacg
    16921 acgttcgcca cacgatctac gtcgctcgca gcgacctcga cggaccgacg ggttcgttcc
    16981 aagccggtca gcgcgcgacg atccggttcc ggttcccgaa ctggctcgcg cacagcacgt
    17041 acacgctcac gcccgccgtc atgaccagcg acggaaggat cgcgctcgac cagcgcatcg
    17101 acgcgctcac gctgctcgtc gacaccaacc gtgtgaccgg tggcgtcgcc gatctgccga
    17161 ccgaggtcga ggtcgagcgc tcgtgagcgt cgccgaggcg gtgcagacgc gcggaccgtc
    17221 ggcgctcgga ggcgacctgc ggcgggcgct cagcctgacc tggatgctcg ccgtgacgga
    17281 cttcaagctg cgctacttcg gctcggtgct cggctacctc tggacgctaa tgcggccgct
    17341 gatgctgttc ggcgtcctct acttcgtctt caccaaggtc ttctcgatcg gcgagacggt
    17401 cgagcactac ccggtctacc tgctgacctc gatcgtcctc ttcacctact tctccgagac
    17461 gacagcgggc ggcgtgcagt gcctgatcgg gcgcgagaac ctgctgcgca agatgcactt
    17521 cccgcggatc gcgattccgg tgtcggtctc gctgagcgcg ctcttcaacc tcgcgctgaa
    17581 cctgatcgcg gtgctggtct tcgcgctcgc ctcaggcgtc gagccgcggc tcacctggct
    17641 ccagttcccg ctcctcctgc ttgcgttgac gctgcttgcg atcggcttcg cgatgctgct
    17701 ctccgttctc tacgtccgct tccgcgacat ccagccgatc tgggacgtcg tcctgcagat
    17761 caccttctac gcgtcgccgg tcctctacgt gatcggcatg ctgcccgaca acatccagcg
    17821 cgaggcgctg gtcgcgaacc cgatcgccgt gatcctcacc cagatgcgct acgcggtgct
    17881 cgactcgtcg gcgccgacgg ccgccgagat ggccggcgtc gacgcgcgcc tgctgatccc
    17941 ggccggcctc gtcgtggtcg tgttctgcct cggtctgtgg ctcttcacgc gcgaggcacc
    18001 gcgaatcgcg gagaatctct gacgtggcac gcacgacgca gcgcgacgcg gcacaggcga
    18061 acggccagcc gagcgacgac gagctcgcgg cgatcgagcg cgagcacgtc gaggaggtgg
    18121 cgcgggcgca tgccgcgctc gccgaggctc aggaccgcag ctactggctc gaccgctggg
    18181 gggtcgacct caacgagtgg atgcgccgcc ccggcgccag cgagctgcgc gccgccgtgc
    18241 gggcgctgcg cggtgtgcgg cgcgcggcga tcaaggcgcg ccgtcagggc aaggcgaagc
    18301 tcgtcaaggc gcgccgggag atggccgccg agcgcgagca ggccgtcccg gcggagtcag
    18361 cgccgaagct cttcgagcgt tcgctgacgc ccaagcgact ggagtcggcg ccggccacga
    18421 agctcgtctt cgaccggctc agcgagacgg acgtcgatgc gatccggtcg agctgcaacg
    18481 tcaacgagaa cgcgatcgtc gacagcgccg acccgatgaa ccagctgcgg ctgctgatcg
    18541 gcttcggcgc gcaccatggg atacccgcgg tcgtcgagcg aaccggcctc agcggcgaca
    18601 tgccgcccga ggacgtgcat gcgatggcgc gcggcagcat ggcgatcgcc ggatcgctct
    18661 actacgccga cctggtcgcc gacgggctcg agacggccgg catgacgctc gaggacggca
    18721 tgcgctgcct cgacttcggc tgctcgtcgg gccgggtggt tcgcgcgctg gcggctgcct
    18781 acccgggctg cgagtggcgc ggctgcgacc cgatcgaggg cgcggtgacc tgggcgagcg
    18841 agaacctgcc cgcgatcgac ttcgtccaca gcccggagcg accgccgctg ccctacggcg
    18901 acggcgagct cgacgcagcg ttcgcgatct cgatctggtc gcacttcgac gagcccgacg
    18961 ccgtcgcctg gctggcggag atgcgccggc tgatccggcc gggcgggcgg ctgctgctga
    19021 caacccacgg ctaccagacg atcgcccacg acgagggaca cggcctacgc cagacgtccc
    19081 agctgcagga agttcacgcc gcgctctacg accgcggctt ctggttcaag aacgagttcg
    19141 gcgagggcgg cgaccacggc gtcgacaact ccggctgggg aaccgccttc ctgactccgg
    19201 agtggctgct ctgggcgacc gtgcgggact ggcgcgtcgt cggcttcgcc cccgggcgcg
    19261 tcgaggacaa ccaggacctg tacgtgctcg agcggcgcta gcgaggcgcg cccagatgtc
    19321 gacgagcgtc tccgtggtga tacccgtcaa ggacggggca cgccacctcg agcggctgct
    19381 cgccgccgtg cgccggcagg gcgagggggt cgagctgctg gcgatcgact ccggctcgcg
    19441 cgatggctcc gtcgagctgg cgcgcgcggg cggcgcgacg gtgcacgaga tcgcccctga
    19501 ggagttcggg cacggtcgca cgcgcaacct cgccatggag ctcgcgaccg gcgacgtcgt
    19561 ctgcttcctc acccaggacg cggtgcccga gccgggatgg ctggcggccc acctcgccgc
    19621 atacgagctc gatcccgagg tcggcgcggt ctacggtccg cacctgccct ggccggacac
    19681 cagtccgatg atcgcgcgtg agctcgacga gttcttcgcg tcgatgtctc ccgacggcaa
    19741 gccgacgctg cagcgcgccg gcgacctggc gttcctgtcg aacgtcaacg cgtcctacgc
    19801 ccgccgctgc tgggaggaga tccgcttcga ggacctcgcc tacgcggagg accaggcctt
    19861 cgggcgcgcg atgctcgatg ccggctgggt caaggtcttc cacccaggtg ccgcggtgcg
    19921 gcacgcccac gactacgggc cgctcgagtt cgcccgccgc tacttcgacg agtaccgcgg
    19981 cctgcgcgag accgccggac acgtcgagcc gctgaagccc ggcagggtgc tcgcggacgt
    20041 gcgcggcggc gtcgcggccg accgccgctg gatggcggag cgcgattggc cgcagtcacg
    20101 ccgcgccgtc tggacggcac gctcgctgct gcaccacggc agccgcaagg cggcggcggc
    20161 cgcagccacg cgtcccggcc ggctgccggc aggggtcgaa cgccgtttgt cgctggaggg
    20221 ccgggcctcg gtcgcgcggg cgaaccggcg cggcgacggg cccccagacc gcgcgacagc
    20281 cgacgcgcgc agccgcgcgc ctgtcgcccc cgtgccggcg cgctacgccg acgtgctgcg
    20341 ctacgcgaag gagggagccg tgccactcgc gccgcgcaat tcgaccgccc acgagcgggc
    20401 gcgcctgcat ctcgccttcg tcgtcccgcc ctttcggcgc ggcagcggcg ggcactcgac
    20461 gatcttcggc ctgatcgccc ggctcgagcg gatgggccac acgtgcaccg tgtggatcgt
    20521 cgatccgggc ggcaacggcc acatcgaccg cccagccgtg acccgccgcg acatcgtcga
    20581 gtggttcacg ccgatctcgg cgcccgtctt cgccggcttc gatgcctggc acggggccga
    20641 cgtcgcagtc gcgaccggct gggacaccgc acatccggtt gcgatgctgc ccggctgcgg
    20701 cgcccgggcg tatctcgtcc aggaccacga gccggagttc ttcgcgacct ccgcggagcg
    20761 gatctgggcc gagcagacct acgacctcgg cctctatccg atctgcgcga gccgctggct
    20821 gcgcgacctg ctcgctgagc agcacggctg cgagggtgcc tacttccacc tcggcgtcga
    20881 ccacgacgtc taccggccgc aggaccacgt cgagcggcgc cgcgacacga tcatcttcta
    20941 cgcgcgcgcg gcgaccgccc gccgcgcggt tccgctcggc ctgctggcgc tcgaggagct
    21001 gtgcgcacgc cgtcccgaca cgcgcgtgat cgccttcggg caggagcggc cgctgaagac
    21061 gtcgttccag accatctcgc tcggcgtcgc cgccccggcg cgcctggcgg ccgcctatgc
    21121 cgaggccacc gcgggactct gcctctcgct gaccaactac tcgctgatcc cgcaggagat
    21181 gctggcctgc ggcttgccgt gcgtcgacct cgcggggggc agctcgcaaa ccgtcttcgg
    21241 agccgacggg cccgtggtgc tcgccgacgc cgacccgatc gcgctggccg acgcgctcga
    21301 ggcgctgctc gacgacgagc gccactggcg agccaagtcg agcgccggca tcgagttcgt
    21361 tgccgacgcc tcctgggaca cagccgcgaa gcaggtcgag gccgggctgc gcgaggcgct
    21421 gcgacgagct gcaatgccgg acggcgcggc gagcgctggt cgacactcgc aggcgcgcta
    21481 gcggcgcctc gcgacctcga cccagtccac cggacggtcg cagagccgtc gccatccgtc
    21541 cggtccgatc tggcgggcca ccgggacacc ggcgtcggcg ggcgcgaaca cgaccgcccg
    21601 cagcacggtc ggcgcggcat cgccaggttt cagcgagcca cccggccgct cgagcgtgag
    21661 catgtgaagc cccgctgtca gccgggcccg cagcggtcgc gcgtagttgc cctcgttgcc
    21721 gctctggccc gcgatgctcc cgatccggcg cccatcgatc gtcacgtcga gccgccgcgc
    21781 gaagtcgccc ttgaaccaga cgtcgtagac gccgctcgcc agcaccgaga accgcttgtg
    21841 ggcgatcccg ggcgtcgcga gtccgatccc gtcgacggtc tcgccccatc ccgctgggcg
    21901 gcggcggatc gactggccct cgaaagccga gaccaggggc cgaggcgagt accagagcga
    21961 tgcgtcgctg gcacgcgccc ggcggccgag cgccgcaagc cgctcgcagg gcacgaagcc
    22021 cgcggcctga aggccctccc ccgccggcac gtgcgcggtc acctccaagt cgcgaacgcg
    22081 ccgccatacg tcgtagtagt caccccgctc ggcgaggcgg aaattcgccg gggggcggct
    22141 ggcgtcggga cggcgccgcg cgacgatcgt cgcgtagccc tggaccgcct cggggtcgag
    22201 ggaatcgagg tcatagctgt gcccatagcc cgtcaccgag ccgtcgcgca gcgtcgcgag
    22261 cgacaccggc ctcgatgaga acccgggacc cgcgggcgcc atgtcggcga gcgcgtagag
    22321 agcgaactcg tcgaagtcgg ggacaagcgt cggaccctgc gccgcgcgct cctccccgat
    22381 cgagagcagc tcctcgaggc gctcggtcgg agcgaggttc gtctcgtggt aggcgagcgc
    22441 attcgaccac aggaccccga gcacgagcgg gacagcgagc gcgacggcct ctatgcgcct
    22501 tcccgatcgt cgcaggctgt ccgcccccag ggcggccagc aggacgacga tcggagaggt
    22561 gatcacgagc agcttcgcgt cggtccaggc cgtcccgcgc agggtcaggg ccacccagac
    22621 ggtggccatg acgacgacga agccggcgag cgcccagctg cgccgacgca ccaacgacgc
    22681 gattcccagc gccgcagcgg cgaacatgat gccgatcagc gcataggtca gggcgaagtg
    22741 ctgggcgggg tccacacggt gagatccgcc gatccagatc ccagccagct gtgcgcgctg
    22801 cagcgggcga acgaggttgc ccggatcgct gacagcgacg gcgttgctcg tcgacagcga
    22861 cttcgcgagc gtgagcgagt ccccgagagc gccgaaagtc ggcagtgcga acaccacgag
    22921 cgcgaccgcg aaggccgcca gccagacgcc gagcgagcgc agatcgaccc gccgcgaccg
    22981 cgacccgatg acgagcagca ggccggcgac gagcagcgtc atgccgagcc acgggaggaa
    23041 tgcgaggccg atggcgccaa cgccggccgc gccgacgagg gccggcagga cggcgccccg
    23101 cggtccgcgc tcgagccagc gcggcatcag caggagcacg ggaccgagca gcagcacgaa
    23161 cgggagcacc gccagctcct tgatcgctcc catctgcgca taggcgtaga cgagcgccgg
    23221 cgcggaggcg acgaccgcgg ccaccgccgc cgctgggcgt cccatcgacg ccgtgcgcgc
    23281 aaggtagtag agcggcgccg cgcagaaagc cagcagcagg gccatgagcg gctggtacaa
    23341 ccagatcgcc ggggtgccga cgagacggct cccgccgccg agcagggtct gcccgccgcc
    23401 gggatagcta agcccgaagt agttctccat cgtcgtgcgg aagctcgact cgggcagccg
    23461 ggcgaagttg cgtgcgtgct cgagcaggta gtccgagccg gcgaggtgga aggcaaccgt
    23521 ggtgtcgagc aggtagcccg cgagcgtgac gtcgccgtgc agcaggaccg gcgcgcagac
    23581 catcacgtag acggcgacgc tcgcgccgac cagccaccag tccggtcatg ccgcccgcag
    23641 ccggctgcga ccggcgatca ggccgccaac tccgaccagg acgaaggcat acgggaccca
    23701 cgacgccgtc gcctcgccgt acgtgcacag ctccgcgacc acgaccagcg cagccagccc
    23761 cacgaccggc agcaggatcc caggcaggac gtcgccgacg atccggtcga tgagcaggcc
    23821 gccaccgagc gcgaggagcg ccagcagcag cgggaacagc actgtcgcga cggcgagctc
    23881 cacgagcgcg tcacgctatc ggaggcggat gacccaatgc ggtggaccga cgactatcct
    23941 ccgcgccttc atgactacca agcgcgcact gatcacaggc atcaccggcc aggacggctc
    24001 gtatctcgcc gagctcctgc tcgagaaggg ctacgaggtc cacggcatgg tccgccgctc
    24061 ctcgaccgag accttccagc ggctgcaggg ctgccgcgac gacctgacgc tgcacaccgg
    24121 cgacctgctc gaccagcgct cgctggtcga cgtgatgcgc gcctgcgagc cggacgagat
    24181 ctacaacctg gccgcgatgt cgttcgtcgc ggcgtcctgg aaccagcccg tgctgacgtc
    24241 ggagttcagc ggcgtcggcg cgacacggat gctcgaggcg atgcgcgagg tcgctcccga
    24301 ggcgcgcttc taccaggcgt cgtcgtcgga gatgttcggc caggtgctcg aggtcccgca
    24361 ggtcgagacg actccgttct acccccgcag tccctacggc gtggcaaagg tctacggcca
    24421 cttcatcacc gtcaactacc gcgagagcta cgacctcttc gcctgctccg ggatcctctt
    24481 caaccacgag tcgccgcgtc gcgggctgga gttcgtgacg cgcaaggtca cccacgccgc
    24541 cgccgcgatc aagctcggcc tgcagaccga gctggcgctc ggcaacctcg acgccgagcg
    24601 cgactggggc tacgccaagg actacgtcga ggcgatgtgg ctgatgctgc agcaagacga
    24661 gcccgaggac ttcgtgatcg cgaccaacga gacgcacagc gtgcgcgagc tggtcgacgt
    24721 cgcgttcgac caggtcggcc tcgatccggc cgaccacgtt cgcatcgacc cgaagttcct
    24781 gcgcccggct gaggtcgagc acctgatcgg caaccccgcg aaggcgaagc agaagctcgg
    24841 ctgggagccg cgcacgacgt tcgaggagct ggtccggctg atggtcgacg ccgacctcga
    24901 gctgctcgcc agcggcgtgc cgcagaagca ggccggctga gttcgatcga gaggcccgcg
    24961 gcatgagcgt ggcaatgaag ctccgggagc tccgctggcg ggcggcaggg cactacagcc
    25021 agttcggccc ggagtggaca tcgccgctcg cgggcgcacg cgggctcgag atcggcggtc
    25081 cgagcgccgt gttccgcggc gacggcatgc tgcccgtcta tccacagctg gccgagctcg
    25141 acggggtcca ggttccgcac gctcacgcgc tctggcacgg cgagcttgag gagggcgcct
    25201 acgagaccgg cgagcccggg ctgagcggcc gcctctggct gcgcgacggg accgggctcg
    25261 accacctgcc ctccgagagc tatgacgcga tcctctcgtc gcacgtgctc gagcacatcg
    25321 cgaacccgct gcgggcgctc gaggagtgga tccgcgtgct gcgccccgga ggcgcgttcc
    25381 tgatcgtcgt gccgcacaag gagggctgcg ccgatcatcg ccgtccgaca acgacgctcg
    25441 agcacatgat cgacgacctg cgtgcgggta ccggcgagga cgacatgacg cacgccgacg
    25501 aggtgctgcg gctgcacgac atcgcacgcg acccgcccgc gcgcgatccc gagacgctgc
    25561 gtcagcgcgt gctcgacaac gcgcgcgtcc gagcggtgca tcaccacgtc ttcacgctgc
    25621 cgtcggccct gcgcgtcctc gatcacgtcg gcctgcgcct gatacgcgcg acggcgcgct
    25681 ggccgcacga catctacgtc ctggccgagc gaccgcggcc cggggaggcg gtcgacaaca
    25741 gcgcactgct cgaccgcggc gcggtggcgg gcagcccctt cacgaccgac cgcgcctagg
    25801 tgggccggcc gaccgtgtcg ccgctcgtaa gcgtcgcgat cccgaccttc aaccggcgag
    25861 cgacgatcgc acgcgctgtg cggtcggcgc tggcgcagac ccacgcgaac ctcgaggtcg
    25921 tcgtcagcga cgacgggtcg acggacggca gcgccgaggt cctgcaggag ctcgcggccg
    25981 gcgacgagcg tctgcgtgtc gtgcgccacg ccgccaacgc cggcatggtc gcgaacatgg
    26041 agcgcgcccg gcggctcgcc agaggcgact acgtgatgct gctcgccgac gacgactggc
    26101 tcgacccgcg ctgtgtcgag ctggcgctgg cggcgctgct gcggtcgccc gaccacgtcg
    26161 cagcgctggg gggcgtcgac tacgtcggca gcgtcgccca cccggtccga gcggaactga
    26221 ggccgctgac cgccgcccac ccgagccggc gcgtgcgcga ctacttcgca gccgtcgacg
    26281 gcgaccacgg caacacctgg ctctacgccc tgatccgccg cgacgccttc gacgcgttgt
    26341 cgccgctgcg caacgtgctc ggcttcgact ggctgcacgt ggcggagctc gcgttcctcg
    26401 gcaagatcgc cctcgtcgac gaggtcctga tccaccgcgc gctcggcggg acgagcgaga
    26461 ccacggcgcg caacgtcgcg gtagccgggc tgccgcgcgc gctcgcgcgg atcccgcacc
    26521 ttgccatcgg aggcgaggtg ctcgcggacg tcggctggcg atcgccggtc tacgctccgc
    26581 tggggcggcc gcgacggctg ctcctggccg ttctctgcgc ggccggagtg ccgctgcgca
    26641 acctgcgcca cgtcctcttc catctcgctc cgcgccggct gcagcgacgc tggcacgcgc
    26701 gatgacgaga tcggcgatcg gcatcgacgc ccgcgccgcg accgaggagg gtgccggcgg
    26761 cgggcggttc gtgcgcgagc tggtccgcgc gctcgcgaag cgcgacgacg atcggcgcta
    26821 tgtcctctat gcgcgccggc gctgggagga gccgctcgac gagcggttcc gctggcgcga
    26881 ggtctccggg cgtgacccga tctggcacgt acggaccgca ctggcggcga accgcgagtg
    26941 cggcgcgttc ctgtcgacga actcgtacct gacgacgtgg ttcctgcgcg tcccgtccgc
    27001 cgccgtcgtc ttcgacctgg tcgctttcga tccagcgttg cgcccgcagc gccgctccgg
    27061 tctaatcgag cggctcacgc tgccgctggc ggcacgccgg tcggcggcgc tgatagcgat
    27121 ctcgcaggcc accgcggacg acctcgtcga acggtttccc accgccgccg gcaagacgac
    27181 cgtcgcaccg cttggcaccg ctccgacgct tggagacgaa gcgcgcgaac tgccgcgcgg
    27241 cgtgcccgaa gggggcttca tcctggcggt cgggacgctc gagccgcgca agaacctgcc
    27301 gcggctcgtc gcggcctacg agcgccttcc gcgggcgctc caggacgccc acccgctggt
    27361 cgtcacgggc cgcctcggct gggacgccgg cgagacgatg gcgtcgctgc gcgcgctcgg
    27421 cgatcgggcg gtcctgaccg gcttcgtgcc ggacgccgag ctcggagcgc tctaccggca
    27481 ctgcaacctg ttcgtctacc cgtcgctcgg cgagggcttc ggcctgcccg tgctggaggc
    27541 gatggccgcc ggcgcgccgg tcctgacgtc gtcgcggtcg agcttgccgg aggtcggagg
    27601 agatgcggcg gcctactgcg acccgaccga caccgccgca atcgcggcgg cgctggagca
    27661 gttgctgcgc gacccacccc ggcgggagga gctcagcacc gccggcccgc ggcgggcggc
    27721 gcagttctcg tgggaccgca ccgcggacgt cactgtcgag gtcctcgagc ggatcagccg
    27781 cgaagcgccc gcacggcgcg cgtgacccgc tgcagcgcgc gccgaccgcc acgcagcggc
    27841 gagacgtcga gcgggttgcg gcgagccagc cgcggcgggc gctggtcggc cgcggcgagg
    27901 aagccgcgca gcggcgccac cgcccgcgac caggcatggc gctccgcggc caggcgcaga
    27961 gccggctcgt agtacggcct tccgttggcg agcactcgct cgattcctga cgccagcgtg
    28021 tccaggtcgc ctggcgcggc gaccgcgccg aggtcctcgg cggcgatctc gtcggcaagc
    28081 gtgtcgccgg agctgacgac cggcggcagc cgtgcccaga agcagtcgag caggcgcgtg
    28141 cgaaacgcga agcgcgtctc gaggtggtcg cggtgcgtcg agatcgccgc gtctgcggag
    28201 gcgagccagt cgccgcgcgc cgcgtagtcg acccacgtgt cgttgaacaa gacagccgac
    28261 tccgggagtc cctcgtcgag cacagtcgcg cgagcggccg ccagcgcctc gccggcgccg
    28321 cctgcgctgc tggcgcccat gaacacgagc cgcgccggga cgccgtgcgc ccgcacgcgc
    28381 gcgagcgcgc ggatcgccag cggcgcgtcg agccagctcc agagtccacc gttccagagc
    28441 aggattcggt cgccgcgctc gacctctggg aaggtggcgc gaatcgggtc gccggcgggg
    28501 cgcggtggcg gctcgtccgg cacgccgaac ggcacgacgt cgagcaccga ccgcagcgtc
    28561 gggtcgcggt cgtagaccgc tggggtgagc aggccgagcg cgagcatcgc gccgagccag
    28621 aggtcgcgct gccgctcact cgcgcagacg aagaagtggc cgctgcgcag ggcgtcgacg
    28681 agccgatcgg tcgcagtggt cgcgtgcagg gcgcgcacgg gagcgctgga tagccggaag
    28741 cgctccagca cctcgaggct ctcggggtcg tagaggtcga tcgccagcgg cgcgccgctg
    28801 cgacggaccg ctcccagcac atgcggcgcc agcggcggag ccaggaccgc gtcggccgag
    28861 cgcagcagct gccgaagcga cccgagacga tccgccgacc acgtcgtgcg ccgaaccccg
    28921 ggaatcgccc cgccgtccag ctcgcggccg gcgagcgtca cctcgacccc gccggcggcg
    28981 agcgcacggg cgatctcgct gtagcggatg cccatcccgg acatccgctc gccgagccgg
    29041 tccgggcaga cgatcagcag tcgcagggcg cgcaacggcg acaacctagc cgaccggcgc
    29101 cccgctcgag cgcagcccgc gcaggatgtc gcgcacctcc ggcgggaagc ggccgagcac
    29161 acgcagcacg accaggaaga ccatgccggc gatcgccgag tcgacgagcg ggtggaagtc
    29221 ggcgaacgcc cccgcggaat acccgcacgc tgccgcgacc aacgccaccg ccaggctcga
    29281 gtagggcatc ccgggaccgt tcgcgcgcac gagcagccag gtcgacgaga tcgcgagcgc
    29341 gagctcggcg atgacagcgg cggcggcggc accgtcggcc tcgaatggcg ggatcaggac
    29401 gatcgcgagg atcaccgcga gcgcgagcga caggcagttg gcgaacagcg tctcgcgatg
    29461 ccgacgcatg ccgagcagca tgtaggtcgc cgccacggcg acgaaactgg gggcgatcgc
    29521 caggctctgg atctgcagca cgacgaccga cggatgctcg gagctgccgg tcaggacctc
    29581 gaccgcgaac ggcgccagca gcatcaggca gagggcgccc agcgcgccta cgtagacgct
    29641 cagctcgaac atcctccccg cggcgaacgc gaagcgctcc ttgtccgagc gcgcggcgcg
    29701 agcgacgatc gggaacgccg atccgatcag cagcagcggg acggtcacca gcacctccat
    29761 cacgcggaac gagatcgcga agtagccggt ctgctcggcg gtcgcgagca gcgtcatgat
    29821 caccagcgtc acacgcagat agagcgtgtt gaccgcgacc gcgagcgcga agatcacggt
    29881 ctcgcgcagc agcgcgcccc aatggcgcgg gttgaacgac ggccgcaggg tggtctcgtc
    29941 cttgaccagc gccaccgtca tcgccagcgc cacgatcccg cccgggatcg acgccgcgag
    30001 gaacgacacc accccggcgt cgagcagcac gagcgcgacg atcagaccag tgatcagcac
    30061 ctggcggatc agctcgatca acgtcatcca gccgaagcgc agcctgctct gaagcaccgt
    30121 ggcgagcaac gactgggtga ccgcgaacag catgccgagc ccggccagga gcgccccctc
    30181 ggtcaggctc acgccgtagc ccgccacgac cacgaagccg acggcgagca ggatgccgcc
    30241 gcagctcagc agcagccgca gcccgagcag gtcgcgcatc gcccggtcgc gctcagcccg
    30301 gtcggtgatc gccgcgagct cgcgcagcgc aacggtgttg acgcccccct cggtcaggcc
    30361 ggcgacgatc gcgacgaccg ccagcactgc ggagtagcgc ccgaacccgg catcgccgag
    30421 gtggcgaacg atcagcggcg ccgaggcgag tccgagcagc agcccgagga cgaagccgcc
    30481 ggtccgcagc aggccgccgc gcagagcggc cggtccggcc tccgccgtgc cgacgatgtc
    30541 ctcagcaccg ggacggtcgg cggccggagc tgactcgatc agcgacacag cgcgatgcta
    30601 cggaccgcat ccggcgcacg gtccgcccgc cgcccgatcg cggcctccgc gccgataggc
    30661 tcgcccgatg acggcggccc cgacgtgctg gctctgcgac gcgccgacca gcccctgtgc
    30721 cgcgctcgcg ccgctgccgt tccggtcctg tgagcgctgc ggcttcgtct ttcgaccgga
    30781 cctctccgag accgcccgcg cgatctacga ggactccgcc tacgagggga cccatggaga
    30841 gatctacacc agccccgagt acatgcgcgc acgcgacagc gacgccgcgg tcaggctgcg
    30901 cttcctacgc ggccacgccc aaggtggcga cctgctcgac gtcggcgcct cggccggcaa
    30961 cttcacggcc gccgcggcgc gcgccggctt tcgcgcgcgc ggcgtcgagc cgaccccggg
    31021 cttcgcccgt gtcgcgcgcg agctgctggg gaccgacgtc gcgacgggca cgatcgagga
    31081 cctcgacctg cccgagcgct cgctcgacgt cgtgacgatg tggcacgtgc tggagcacat
    31141 cccccacccg acgcgcgagc tgcgccgcat ccgcgcggcg ctgcgcccgt ccggcgtgct
    31201 ggcgatcgag gtgccgaacg cgggcggcgt ggcggcgcgg accgacggca cggcgtgggg
    31261 ctcgctggag cccgacgtcc acgtcaacca gttcgctccc gagacgctgc gcctggcgct
    31321 ggagcgggcc ggtttcgcga tcgcggtgat cgagaccgtg ccgatcacgc cgtacctgcc
    31381 gccgcggcgt cgcctcgccc ccaggcacct cgcggcacga gcgaaggccg cgctgcgcgc
    31441 cggcggcctg cgcggtcgtc atccacacgc gcacgagctg ctgcgcgcgg tcgcgacacc
    31501 ggccgagcgc tagcccgcca gcgctcgctc gtagacggcc agcgtggccg acgccgtcgc
    31561 ctcccagtcg aagcggcccg cctgtgccgg gccgtccctg cgcagccgcg cccgcagcgg
    31621 ctcgtccttc aggaggcgga cgatcgccgc gccgatcgcc gcgccgtcct cgggatcgaa
    31681 ggtcagcgcg gcatcgcccg cgacctccgg cagcgacgag cggtttgagc aggcgaccgg
    31741 cacgccacgc cgcatcgcct cgagaaccgg cagcccgaag ccctcgtaga gcgatgggaa
    31801 cacgaacgcg gtggcggcag cgtagagacc ctcgaggtcg gcctggtcga cccagtcggg
    31861 gaaccggacc tggtcgctgg tcccgaggta ggcggcgcgc ccgatcaggt cggcctcgtg
    31921 cggggtgctg tagccgggca gcacgagcat aggccgctcg gccgcgccga tcttcgcgtg
    31981 cgcctcgagc agccgcatca ggttcttgtg cggccgcttg gccgatgcgg tcagcaggat
    32041 cggccggtcg ccgaggtcca gcttggcccg cagctcgggc tccggtgtcg gtgcgaccgc
    32101 cggctgctgg ccgactccga gcgggaccac gtcgatcttt gcgcgcggaa cgcccaggtg
    32161 gcgctcgacg tcgacggcgg tcgacgcggc gtcgacgatc acccgctgcg accgccgcgc
    32221 ggccgctggc acgagcaccc ccatcccgag gcgcagcaac ccgaagtggg cctcgggcac
    32281 catcttgtag ttgaggtcgt ggactgtcgt gacgcgcttg aagcggcccc acaccggcgc
    32341 ggtcgacgca aggctgtgga cgaggtcgca gccggcgcga gcggccatcc gcggcagatg
    32401 ccgctgctcg ccccagaccc actggaagcg actgcgcgcg tcgaccggca cggtctcgtg
    32461 cggaatcgca tgccatggcc ccggctcgcc cgcggcggcc tcgcggttga cgaaggcggt
    32521 cagccgcagt ccctcgaccg cggccaatcg cgggatcagc tcgcgtgcgt agacctccat
    32581 gcccccggtt tccccaggca cgaggtagac gaggttgaga ccgacgtgca agctggcggg
    32641 cgtcggctag cccgcggcgc gggcagagtc ctggcgccgc aaccccagga tcgacagcag
    32701 gaacgacgag aagacgatct ggatgccgac gatcatcagc gtcgcggcca tcaccgccag
    32761 gcgggtctcg gacagctcgc cgaagccgcg gtcgatccag gtgacggcga tcaccgccgc
    32821 gatggcgaag ccgccgagca ggatcccgcc gccgagcagc agcccgtgct cgagccggaa
    32881 acggcggcgc atgcggtcga accaggggtc ctggtcgccc atgaagtagg tcccgtaggc
    32941 gtgcgcgcag agcccgagcg cgagcacctg ggtcccgacg atcatcagca gcgcgccggc
    33001 gatcagcgcg tggacgtccc actcgcggcc gaagaggttg atgtcggcga gcaccgtgag
    33061 cgcgaccagc gtgccgagcg cggccatcgc cgtccccggg atcacgaaca gccaggtcgg
    33121 gctgtggacg agcaggaagc gcaggtggcg ccagccgtcg cggaagctcg agagcttcga
    33181 ctcgccgccg cgcgggtggt actcgatcgg gatctggcgg atgtcgaggt cgcgcttgga
    33241 ggcgcggatc accatctccg aggcgaactc catcccggtc gtgcgcaggt cgagcgtcgg
    33301 cagcacgtcg cggcgcagcg cccgcatgcc gcagtgggcg tccttgacgc cggtgcggaa
    33361 gaaggtgttg aggatcccgg tcagcaccgg gttgccgacg tagcggtgca gccacggcat
    33421 cgcgcccggg tggatgttgt ccatccggtc gccgatcacc atctgcgcgc cctcgtcgag
    33481 cttggcgacg aacgccggga tgtcctcgaa gtcgtaggtc aggtcggcgt cgagcatcac
    33541 gacgtagtcg ccggtggcgg cggcgaggcc ggcgaggtag gcgctgccgt agccgcgccg
    33601 cggctcgtgg atcactcgcg cgccggcctt ggcggccagc tccgggctgc catcctccga
    33661 cgcgttgtcg gcgacgacga cctcgcccgg gatgtcgtgc tcggcaagca cagccagcgc
    33721 gcgctcgacg cacgtctcga tgttctcggc ctcgttgagg cacgggatga ccaccgacac
    33781 cgacgcgcgg cccgactgcg agtcgccccc tgcggggcga ggagtggtct cgatcgtgct
    33841 caccctgcgc cgcttagggt accggccatg cgcgtctgcg tcgtctacga ctgcctgttc
    33901 ccgcacaccg tcggcggcgc cgagcgctgg taccgcgagc tggcgctcca gctggcggcg
    33961 gacgggcacg ccgtcaccta cctcacgcgc aagcagtggg acgacgggga ctcgccgcag
    34021 ctgcccggcg tccgcgtgat ccccgtgtcg ccgggcggac cgctctacac cgcggacggc
    34081 cggcggacga tcgggccgcc gctgcgcttc gggctcggcg tgctcaggca cctgctgcgc
    34141 aaccgccgca gttacgacgc cgtccacctc tgctcgttcc cctacttctc gctgctcgcc
    34201 gcgcggctgg cgctcgccgg gactcccgcc cggatcggcg tcgactggtt cgaggtctgg
    34261 acgctcgact attggcgcgg ctacctcggc cggatcggcg gccggatcgg ctggctcgtg
    34321 cagcggctct gcgcgcggct gacgccgcag gcgttcgtgt tctcgcgagt gcacgccgag
    34381 cggctgctcg gcgagggcct gcgccggccg gcgctgcggc tcgccggcct ctaccgcgga
    34441 ccgctcgaac ccgcagcggc gggcggcgag cgggcaccgc tggtcgtctt cgccggccgt
    34501 cacatcccgg agaagcgcgc cgaggtggtg ccggccgcgg tggccgccgc acggaagcag
    34561 gtgccgggac tgcgcggcct cgtgctcggc gacggccccg agcgcgagcg ggtcctcgcg
    34621 gcgatcgccg cagcgggcgt cgaggacgtg gtcgaggcgc ccggcttcgt gagcggcgag
    34681 gacgtgtcgg aggccttcgc gcgcgccacc tgcatgctgc tgccgtcgtc gcgcgagggt
    34741 tacgggatgg tggtgatcga ggccgcggcg ctcggcacgc ccagcgtcgt cgtcgccggc
    34801 gccgacaacg ccgccgcgga gctgatcgag gaggacgtaa acggcttcgt ggcgcccgcg
    34861 accgagccgc gagcgctggc gaccgcgatc gtcgcatgcc acgccggcgg agccgagctg
    34921 cgcgagcgca ccgccgcatg gttcgcgcgc cgcgctccgg agctgaccgt cgacgcgtcc
    34981 gcgcacgcgg tggcgcgctg gtacgcgacg gccgcgcgct agcgcgcccg agcgggtatg
    35041 cccatcaccg tcgtgccggc ctcgacgtcc cgggtcacca ccgcgccggc ccccacgacg
    35101 gcctcggcgc cgaccacgat gccgggcagc agaatcgccc cggcgccgat caccgcgcca
    35161 ggctggatcg tcggtccggc caaccgagtg tccccactca gctcctcggt cggcgtgttg
    35221 tcattggcca tcgcgaccat cgtcgagacg aacgccccat cgccgatcgt cgtgcgaccg
    35281 gtgacatgag tgttgtccat gattttcacg tcatcaccga tcgtgacctc gtagttgagc
    35341 gtcacgcagc gtgacacgat gcagcgggcg ccgatgcggg ccccctcccg gatcgacgcg
    35401 ccgtcgccga ccagcgtccc cggcccgatc tggacgtcgt agtagatggc cgccccaacg
    35461 ccgatcgagc agtcgtcgcc gacgaccagt tcacgcgcaa acgacggggc gcgcgcggtc
    35521 gcgccggcgc ccttcggctc gcgacctacg acagcgcccg ggaacacttc gacgcgggcg
    35581 ccgagcgtcg cgccggggcc caccacggag tgggagtgca ggacgcactc ctccccgatc
    35641 cgcgccccgt cgccgacgac ggcgaacgga tcgacgcggc acgacggact gacctcgcct
    35701 gcgatcgcgg gcgtcgcgct catactttcc acacgggcag catgaccgac tcggcagccg
    35761 gaccggcggc gaagcgttcg caccggctca cgagggcgcg gacgccgccg ccgcgacgtg
    35821 ctcgcggtac cactcgatcg tccgtcgcag cccgtcctcg aagtcggtcg aggcggtgaa
    35881 gccgagcagc tcgcgggcac ggctcgtgtc caggctccgg cgcggctgcc cgtcgggctt
    35941 gctggtgtcc cagaccagct ctccctcgaa gccggtcagc cgtgcgatcg tctcggtcag
    36001 ctcgcggatc gtgatctcgc gcgccgcgcc gatgttgatc gggtcgggac cgtcgtggcg
    36061 gcgcgccgcc gcgacgatcg cctcggcgca gtcgtcgacg tagaggaact cgcgcgacgc
    36121 actgccggtg ccccagcaga cgacctcgcc gcggccggct acacgcgcct cctcgcactt
    36181 gcggatcagc gccgggatga cgtggctgga ctcgaggtcg aagtcgtcgc cggggccgta
    36241 gaggttgacc ggcatcaggt aggcgctctg caggccgtac tgctggcggt aggcctgaag
    36301 catcacgagc agcgccttct tcgcgatccc gtacggagcg ttggtctcct cggggaagcc
    36361 gttccagagg tcgtcctcga cgaacggcac cggcgtgtgc ttcgggtacg agcagacggt
    36421 gccgacctgg acgaacttct cggcgccgcg gtggcgcgcc tcctcgatca ggtgcagccc
    36481 catcgccatg ttggcgtaga agaagcggcc ggggttgtcg cggttggcgc cgatcccgcc
    36541 gacctcggcg gccaggtgga cgaccgtctc cggacggccg tcctcgtagg cgcgcgcgac
    36601 ggcggcggcg tcggtcaggt cgtagtccgc gcgccgcggc acgaagacct ccgccgctcc
    36661 ggcggcgatc aagcgctcgc gcacctgcgc gccgaggaac cccgagcccc cggtgagcat
    36721 cacccgcttt cctgtcacgc cgtcggccac agtctgagca ggctagcgcc gcggcggccg
    36781 ggtcaggatg gcgcgaacca gctcgcgctc gccgcggtcg agccagacga ggaagaaggc
    36841 ggcccagtag gcgagcagcc ctccgagcgc gagcgcaccg acggcgagcg cgctctgcgg
    36901 gtcgagcgtc agccgcgcgg cgaccagcgt cgccgcgagc agcgcgccga gcgcccaggc
    36961 cggcgcgaac gcccgccgca gcagctcggc cggggcgacg ggaacggccc gcagcgtgat
    37021 ccggatcagg tacgggaaga gcgcgacgta cgggatcgcc gtcgccagcg cgacgccgtc
    37081 gaggcctagc ggcggcgtca gcgcgagcgc gagcagcacg ctcgccgccg ccaccgcgac
    37141 cgcccagcgc gcgaggtcgc gcgccgcccc gaccgcgacc agcatcgccg cggcgacgcc
    37201 gctcatgccg ttgaacaccc agtggctcat caggatcgcc atcgccgatc ctgcctcgcg
    37261 gaaggccgag ccgagccaga cgtcgaggat cggcccggcg agcgccatcc cggtcaccgc
    37321 gatcgggacg atcagcgcca gcgtgtagcg cgagccgcgc acgagcagct cgcgctgacg
    37381 gcggtcgtcc ccggacgcct ggtagcccgc ggcggtcggc aggacggtca ccgtgaccgc
    37441 cgcgttgagc gcgcggatca ggttgtgcgc gcgcaccggg ccctcgtaca gcccgaccgc
    37501 ggcggccgac ttgaacacgc cgaggatcag cctgctcagc gcgtagacgg cggcgctggc
    37561 cgcctcgctg agcgagatgt agccggctag cccgagcatc tcgcggccga ccgcgcggct
    37621 ggccgaccgc ggccgcagcc gccacggcag ccgcgccgcc cacgccgcga gcgcgcagcc
    37681 gaggccggcg agcagcggga tcgccgcgcc cgcgccgacg atgctccaca gcggggcgtc
    37741 ggcgagcgcg aggccgagca cgagcgcgac gtaggcgagc agcgcgacga tctcggttcc
    37801 ggctgcgagc acgaaccggc cacgcgcccg cagcgcgtcg cggtagacgg tcagcggcca
    37861 gccggcgagg gtgacgagcc cgatcgcgag ggcgccgaga cgcgcctggc ggcggacctc
    37921 gtcggggagg tcgagcgcct cggccagccc gatcccgacg acggcgagca gcagcccgct
    37981 cagcgccccg gcgacgacgt agagcagcgc cgccgtcgag aacgccgccg acccggcgcg
    38041 cgcgtcggcc gccgccgcca tcgtccgcac cgccgccccg gccgcggcgt tctggacgat
    38101 cagcaggtag ccggcgagcg cgttcagcag cccgtagacg ccgagctcgg ccagcgacag
    38161 ccgccgcgcc agcaccgtga tcacggcgaa catcgccacg agccccgtca cctgcgccgc
    38221 ctgctgcgcg agagaaccgg tcgcgagccg gcgcgcatcg ctcatgggcg tatcttctcg
    38281 cgcgtgagtc ggacgccttc cgccgagacg gttcccccac gcgccggcgc ggcggcgggc
    38341 gtgcggcgcc gcgatgtgct cggggtgccg atcgcgctcg tcgactacgc gcgcgcgctg
    38401 gacgtgatcg acgcaatggt cgcgcgccgg gagcgcggtt ggatctgcgc cgcgcccgcg
    38461 atgtcgctgg tgaccgcgca aaacgacccg ctgctgatgg cggcgctgcg cggcgcgacg
    38521 ctgacgctgc ccgacgggat gccggtcgtc tgggcggcga acctgctcgg ccgtcgcagc
    38581 ggcggcgagc ggctcgacga ccgggtctac ggcccggacc taatgcgcta ccactgccag
    38641 cgcgccgccc gcaacggcca gcgcgtctgg ctctacggcg gtcacgacga cgacgcgctg
    38701 gcggcgctcg aggcgagcct tctgcgcgac catcccgggc tgcggatcgt cggctcgtgg
    38761 tcgccgccgc acagcgcgcc gaccgcggcc gaggattgcg agatcgccga gcggatcgac
    38821 gccgacgcgc ccgatgtcgt ctgggtcggc ctcggcatgc cgaagcagga gcgctggatg
    38881 gcgcgactgc gcccgctgct cgaggcgccg gtcctctgcg gggtcggcgc ggcgttcgac
    38941 ttccacgccg ggcgggtggc ccaggcgccg cgctggatgc aggagcgcgg cctggagtgg
    39001 gcgcatcgcg tcgtccagca gccgcgccgc cagctcccac gctatctgcg ctcgaacccg
    39061 cgcttcgtcg tgggggtcgc gcgccagtac ctgcgcgagc ggcggccttg actccggcgc
    39121 tcgtcaccgg cggcggcggc ttcgcgggcc ggcacctcgt cgagctgctg gccgacggcg
    39181 tcgaggcgcc gccaagctcg gagctcgacc tctgcgacgc cgtcgcggtc cgcgcgcggg
    39241 tcgccgacct gcaacccgat gccgtcttcc acctcgcggc gttcagctcg ccgagcgagt
    39301 cctggcagcg cccggaccag ggcctgctga ccaacctcca gatgacgctc aacctgctcg
    39361 aggcggtccg ccacgaggcc ccgcgcgcgg cggtcgtgct cgtcggatcc gggcaggtct
    39421 acggcgaccc ggagcggctg ccgatcgacg aggacgcggc gctcgcgccc ggcaacccct
    39481 acgccgtcgg caaggccgcc tcggacatgc tgggccgcca gtacgccgag gcgttcgagc
    39541 tgcgcatcgt gcggttgcgg ccgttcaacc acgccggtcc cggccagcgc gacgagtacg
    39601 tgctcagctc gctcgcgcgc caggtcgccg cggcggaggc ggccggcgcg accgagatga
    39661 cgctgcgcac cggcaacccc gagtcgcgcc gcgacttcac cgacgtgcgc gatgtcgtgc
    39721 gcgcctacgc gctggcgagc gcgctcgacg gcggcgcgtt caacgtctgc agcggccgct
    39781 cggcgtcggt ggcggagctg gtcgctctgg tcgccgggca cgcgcagatc ccggtccgac
    39841 acgaggtcga ccccgcgctg ctgcgcgacc acgacgcgcg cgacatgtgc ggctcgcaca
    39901 tccggctgac ggaagcgacc ggctggcagc cggagatccc gctcgagcgg accgtgcgcg
    39961 acacgctcga ctggtggcgc gagcggctca gccgctgagc ccggcgtaga gcccgagcag
    40021 gtcctcggtg tagcgctcgc gcgaatggcg ctcgcgcgcc cgcgcgatca acgcgtcgcc
    40081 ctcggcggcg cgcccgacgg ggtcggccca aagcgcagcg acgcgcgcgg cgaacgacgg
    40141 tatgtcgccg atcgcgacgc agcggtcctc cccgagcagc tcgggaaggc cgcccatcgc
    40201 tgtcgcgacg acggggacgc cggcggccat cgcctcgagc gccgagtacg gcgagaactc
    40261 gtggtagcgc gacggcatca gcaccattcc ggcgccgccg agctcgcgac gcacgtcggc
    40321 ggcggacagc cggccgagga aggcgacgtc ggcggccgcc ggggggctcg tcggcgcatc
    40381 agaggccctc gcagcggcgg cgaccgtcgc gcccgcctcg cgcgcgagcc gctccaggcg
    40441 ttcgcgctcc ggcccctctc cggcgatccg cagcggcacg cgcgccgtca ggcaggcggt
    40501 cagcgcgtcc tcgatcccct tctccggcga gaggcgggag gcgacgaacg cgtagccgcc
    40561 gtcgccagcg gcggagcgct cggcgaacga gtcggccggc aggtagtggc gcagcacctc
    40621 gaggcgctcc gccggcagcc cgcgggcggc gagctcgtcg cgtgcggccg cgctcggggc
    40681 gacgaagcgg tcgaccgcgt cgagcaggcg cggctggtgc agcgacagcg ccgccgtgta
    40741 cgcgacggcc tccggcagcg agcgccggca gttcagcacc agccccggca gggtgttccg
    40801 tccgcgacag cgccggcacg gaccaccgtc gcgctcgccg aagccggtcg agcagaagat
    40861 ccgcacgttg tgcaggtgca gcacgacccc cgctccggcg tcacgcgcag cggccagccc
    40921 ggcggggccc accagcggca cggtgttgtg cgcatggacc agcgccggcc caccgcccgc
    40981 gagccgccga accgcgtcgg ccagctcctg cgggtcggcg ccgcagcgca gcagggcgcg
    41041 cgccgcgccg gccctgccga cgtcggcgga gcgtcgctcg aagctcgcgt gctcgacccc
    41101 ggctgcctcg agcgcgcgca gctgcaggtc cacggaccgc tcctcgccgc ccgcgatccg
    41161 gtagcggttg tgcactacga gcacgcgcgg gcctgcggac accgcggcta acctaccggc
    41221 gctatgcgcg agccggccgt ctccgtcgtg gtgccaaccc gcaaccgcgc cggttatctg
    41281 gaggtcacgc tcgcctcgct cgccgcgcag gcgcccagcg tgccctacga ggtgatcgtc
    41341 gtcgacgacg gctcgagcga cgcgaccgcc gaggtggtcg cgggcgccgc aagcctcgcc
    41401 caggcgcccg atgtcggatc ggcgcgccca ggcgatgcga atccgacatc tggcccgcgg
    41461 gtcgcctacc tccgccacaa aacgtcccgc ggtcccaacg ccgcgcgcaa cgcgggcatc
    41521 gccgcggcgc gcggagcgct gatcgcgctg atcgacgacg acgtccgcgt cccgcccgac
    41581 tgggtcggcg agctggccgc aggcgccgaa cgccaccgtt gggcggaggc gctcggcggc
    41641 ccgatccgag cgagcctcga gggaccggcg ccgcgctcct gcgggcgcga gtccgctccg
    41701 gtgaccacgc tcgacctcgg tccgcgcgac tgcgagaccg acttcgtctg gagcgcgaac
    41761 atggccgtcc ggcgcggcgc gatcgagcgc atcggcggct tcgatgagca ggtgccgatc
    41821 tacggcgacg aggaggagtg gctgatccgc ctgcacgcca acggcggccg cgtcgcgtat
    41881 gtcgcgggcg cctggctcga gcaccgccgc gcgggcgacg acgcgcgcct gcgctcgctc
    41941 gcacgcgccg agtaccgccg cggacgcgcg gcgcgccgca acgaccggcg caagggcacg
    42001 gcgcagccgc tgcacggcga gctgcgcgac ctcgcgggcg ccggttggca caccgcccgc
    42061 cgacgctgtg cccagggcct gatcatgggc gcccactcga ccggccggct ggtcgaggca
    42121 ctgcgcgggc gatgagcgag gccctgcccg acccagcgag cttcctctcg ggcgagtccg
    42181 gcaccgtcgc cgggaagaag ctgaccgtca cgcggaccgc cgcggactgg acctgcaacc
    42241 tggcgggtct ggcggcgctg accgggccgc ggctcgaccg gctcgccgct gtccaaacgc
    42301 cacggcgggt gctcgtgctg tcgatctacc ggcctggctc gctgttggcg actgcggctg
    42361 cgcgcctggg ctccgaccgc cacgacgtca ccttcgcctt cggcgcgacc gccgagcccg
    42421 accccgccct cgcgagcgac accgtgctgg atggactcgc cggaggcaag ttcgagaacc
    42481 tcaaccggct gctcgcagcc tcgccgccgc tcgacgactt cgactggctg atcgccgccg
    42541 acgacgacgt cgagctcgcg ccgcgctttc tcgaccgcgc actcgcgctc tgcgagcggc
    42601 tcggcctcga catcgcccag ccggcgcaga cgatgcgcag ccacgccgcc tggcgcgtca
    42661 cccgccgccg gccgttctcg ctcgcccgcc ggaccgacta cgtcgagatc ggtccggtga
    42721 cgctgttctc gcgccgcgcg gccgccgagc tgacgccgtt cccgcggctg cgcttcggct
    42781 gggggctcga caaccactgg ggcgcgctgg cgcgcgagcg cggctggcgg ctcgggatcc
    42841 tcgacgcgct gcccgtgcgc cacgagtccc agaaggtcgc gacgacctac acccacgccg
    42901 aggcgatcga cgagggccgc cgcttcctcg ccggacggcc gtacgtgacc gccgcggagg
    42961 cgcagcggac gctcgcgacg atccggcgga tcccctcgtg agcgcccgcc gcctgcggat
    43021 cctggtggtg ccgaagtggt atccctggcc cgaccggccc gtcttcgggg ccttttgccg
    43081 cgagcacgcg cgcgccctgg cgacccgaca cgacgtcgtc gtgctcgcgt cgctggcgac
    43141 gccgaggccc ggcttcgcgg cgttccagct cgaggacgcg gtcgaggacg gggtccgaac
    43201 gatccgcgtc cgctaccgcc ggccgttcct gcgcccggcc gcgatgggct tccagctggt
    43261 cggcatgctc gccgcactgc gccgcctgcg ccgcgacggc tggcggcccg acgtcgttca
    43321 tgcccacgtc ttcgaggccg cgccgccggc gctcgcgctc ggccgcctga gccgcgcccc
    43381 cgtcgcgatc accgagcact acacgggctt ccagcgcggc ctggtcacag gctatgaccg
    43441 actgctcgcg aagtggtcgt tcgagcgcgc cgatctcgtc gcgccggtca gcgagcagct
    43501 cgccggcgac ctgcgcgcgc tggcgccgcg cgcccgcttc gaggtcgtgc ccaacgtcgt
    43561 cgacacgtca gcgttcgcgc caccagcggc ggccggtgag cgtggagccc cgcgcctgct
    43621 caacgtcgcg gcgctcgccg agaagaaggg gcaccgctac ctgctcgacg cgctcgtcga
    43681 cttgcccgat gcaacgctcg acatcgtcgg cgacggcgag ctgcgcggcg acctggaacg
    43741 ccaggcgaac gcgctcgcgc tcggcgaccg cgtccgcttt cacggcgagc gctcaaagga
    43801 ggaggtcgcc gcgctgatgc gcgccgccga cctcttcgtc ctcccgagcc tcgccgagaa
    43861 tctccccgta gtcctgatcg aggcgcaggc gagtgggctc cccgcggtcg ccacctccat
    43921 cggcggcgtc ccggagctcg tcgacgacca ggccggactg ctcgtgccgc cggcggaccc
    43981 cgcggcgctc gccgccgcga tccgcgccgc ctccgagcgc gacttcgacc cgctggcgct
    44041 cgccacccgc gcccgtgatc gctacggcta cgacgccatc tgcgcccgct ggaccgcgat
    44101 ctacgagcgg ctactcgcct gaaccgccgc cgcgcgaacg attctcgggg ccataggccg
    44161 aggctacgac cggccgggcc tcaacgcgaa ccgaggagcg cgggcaggac acgctcggcg
    44221 acgatcttgc gcagggcggc gtccgagtag ggctcgagcg cgcggcgcac cgcgacgccg
    44281 taacgcgcac gctgctcgtc ggtcatcgcg aggccggtgc gcagcgccgc cgcgagggcg
    44341 ggggcgctgg cgtcggaggc gatcagctcg gagccgatcc gccgggcgat cgggagcgcc
    44401 gcgttggggc cgggcgtggg caccgtgacc agcggcgtgc cggccgcgag cgcctccatc
    44461 tgagcgacgc cccagtcctc gtagcgagcg gcgctcagat acacacgcga ccccgccacc
    44521 agcgcagtcc agcgttcgcg cgggacggcc ccgacgaact cgacgtccgt cggctcggcg
    44581 actccgcggc gcgcgagcca gcgcagcgcg tcctcgtggc cgatgccccc gacgaccagg
    44641 cgcgcacctt ccggcgccgc tgcgcgccac gcctcgcaga ggacgtcgag cccgcgcttc
    44701 tcgggattcg ccgcgtaggc ggtcgcgtcg ggcgcgtcgg cggcgacccc gccggggagg
    44761 tcgaccacgc acggaagcac caccacgtcg ggtggcgagg gcgccgggcc ggcggcgacc
    44821 gctcgcagcg ccgcctcagc cgcggcttcg ctccacggca gcaggaggtc ggctcgcccg
    44881 aggactgccc gctcccgccg ccgctgccag gcgccgccta ccccgggccg actcagcgcc
    44941 gcgatcgtgt cgaaccggac cgcatgcgga cggcgcagcg gctgtagcag cgccgcggtg
    45001 atgctcgagt agacgaccgc gcgcgcgtcg acgccactcg ccgcccgccg cgcagcaagc
    45061 gcctgcagca catcggtcgt cgccatcgag cgctgcaggt gacgcgccaa gccgagccgc
    45121 atcggccgca gctcgcaggt ggcgccggcc gcctcgatct gcgtcgccag cgcctcgtcg
    45181 ccgcgccgcc agcccatcgt cgtcccgagc gagacgatcg cgatgtcgac ggcggccacg
    45241 gccgcagagt atgcgcggcc tcgccgggct atgttccgcc cggcatgtcc gctgacccgc
    45301 gcccggcctg cctgctgacg ggcatcgtct ccccctaccg ccgcgagccg ttccggctgc
    45361 tcgccgaggc agagcgagtc gaggtgatcg cccacgagca cgccgggccg ccgatccccg
    45421 gcctgaccgt ccaccgcaca tcggagatcg gcgccgtccg gctcgcagcc tcaggccgct
    45481 atcgagcgat cgtcgccggg ctcgacgggc gggtcgcact gcccggcgcc tacgccgccg
    45541 cgcgggctcg cggcgtgccg ttcgtcctct gggcgacgat ctgggcccat ccgcgaacgc
    45601 cggcgcacgc gctctcctac ctgccgaccc gccatctcta ccgagcggcc gatgccgtgg
    45661 cgacctacgg cccgcatgtc agccgctacg tcgagcgcta tcgcggcacg gacggcggcg
    45721 tcgtcgtggc accgcaggcg gtcgacgtcg accacttcgg cgcgccggtc tccgacgccg
    45781 agcgcgtcgc cgcgcgcgag cgcgccggcg gtccgggcac cgagctgctc gtcctcttcg
    45841 tcggacgcct ggtgcgcgag aagggagtag cgacgctggt cgccgcctgg cggcgtgccg
    45901 gcctcgcgcc agacgcgcgg ctcgcattcg cgggcagcgg cccgctcgac ggcaggatca
    45961 ggcgcgcggt ccccgaggcc cgcctgctcg gtcaggtcgc agccgacgag ctgccggcgc
    46021 tctacgcggc cgccgacatc ctcgtcctgc cctcgatccc caccgcatcg tttcgcgagc
    46081 cgtggggtct cgtagtcaat gaggcgatgc tgcagcgaac ccccgtcatc gcctcggatg
    46141 ccgtcggcgc agccgcgggc gggctcgtcc gggacgggcg caacggcttc gtcgtcgcgg
    46201 cccgcgacgc ggacgcgctc gccgctcgcc tgcgcgtcct ggccggtgac gccggcgtac
    46261 gggagcggat gggcgcggca gcacgggagg acgccgccgc tcacaccccg gcggcatggg
    46321 cggcggggat ggcgcgcgcg ctggccgcgg ccggggcggg acgcgccgac agcggaggcg
    46381 attgctagcc tgacacgctt cggcgagcag gaggcggttc aggttgacta gctccccgaa
    46441 tcctcgcgtt atgacaagag ccctccgaca gtcgatgctg cccactgccc tggcgctgat
    46501 tgcgctgctc gtgccagccc aggcggcgag cgccagcgtc ctcgacgtgc agcgcgactg
    46561 cgccgacagc gacagcttcg agcgcaagca ctcgcgcgcc gacctcaaga agacgctcaa
    46621 ccaggttcag gcggaccttg ccgagtacgg cacctgccgc gagatgatcc tcgcggcgct
    46681 cgcgagcatg aagggcggca agggcgcctc gtcgggcaag ttcgccggtg tcgacccgga
    46741 cctcaacaac gacggcgtca tcacgccgaa ggagcggcgg atcgccgcca agaaggcgaa
    46801 ggagcttcgc gagaagcgcg accgccagat cgcggccgtc gacgacgatc tgattcacga
    46861 cggcgacagc ggcggcgtgg cgtccggcgg gggcggctcc gacggcccgc cgctgccgct
    46921 gatcctcgcg ctcgtcgcac tggcgtgcgc gggcgccggc ggcggcctct ggtatgcctc
    46981 acagcgcaac ccggccatcg ccaacgcgct ccgtcgtgtc cagcttccgt tccgcaacag
    47041 ctagcgcagc cctcgcggcg gccctgctgg gggtagcgct gggcgcgcgc ggtggcagcg
    47101 ccctcgagcg caccgcgatc gtcgagctgc tgctgatcgc cgccggagcg cttgccgtct
    47161 gtgccgcgat cgcgatcacg ccgcgcggtc gaatccacgg cgccgccgcg gtcgccgcct
    47221 tcgcgctgct cgcagcgatc accgcgctct cgatcgagtg gtcgattgca ccggagctgt
    47281 cttacgtcga ggccggccgg acgctcgcct acttcgcgac cttcaccggc gcagtcgcgc
    47341 tcgctcggat cgccccggac gggaccgtgg cggtcgcgcg cgcgatagtg ctcgccgcgg
    47401 tcgcgatctg cggctacggg ctggcgacgc gtgtttggcc ggcgtcgttc gacgagctcg
    47461 ccttcgcggg gcgaatcgga cagccgttcg actactggaa cgcacttgcc gggatggcgg
    47521 ccctcgggat cgtgccggcg ctctggctcg gcgcgcgccg ggacggcagc gcgcttgggc
    47581 gagcgctcgc ctttccagcc accggcgtcc tagtcgcgac gctgctgatc gcccagtcgc
    47641 gcggcgcgct cgccggggcc gcggtcggct gcgcagtctg gttagccatc gtgccgccgc
    47701 tccgaggacg accaggctgc gcaaacgcag cggcacgatg gccaaccaga ctgcgcagcc
    47761 gaccgcggcc ccggcgagcg cgccgcgcga ctgggcgatc agcagcgtcg cgactaggac
    47821 gccggtggct gg
//
LOCUS       DATABV010000016         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2255375, whole genome shotgun sequence.
ACCESSION   DATABV010000016 DATABV010000000
VERSION     DATABV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255375"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VHF88_00805"
     CDS             <1..875
                     /locus_tag="VHF88_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX2160346.1"
                     /translation="ANVFDTQVAAGFLGMGNQEGYESLARRVLGVELRGGEGFTRWDK
                     RPLTERQLAYAADDARCLLALGDAIARELEAVGRLEWAREECRIVEQSTDVRSPQQAY
                     ERLPNTRQLDGGRRAVAWALCEWRDAAARELDRPPSYLLPDQVLTQLARRRPTDKHGL
                     EQIRGLPQQTLHRRGGEVLDVIASAARAEPPPRPPAGPQRDPVDQPVVSLAQALLRHR
                     AREAGVATELIATQSELTALVAAVRAGDAGDGIRALGGWRRELVGEELCELVSGRLAL
                     SVGPDGSLVVAHAD"
     gene            complement(852..2426)
                     /locus_tag="VHF88_00810"
     CDS             complement(852..2426)
                     /locus_tag="VHF88_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2160347.1"
                     /translation="MNVAPAVKAAAAAAALALALTLVVAAGAAAQTARPNVVVIMTDD
                     QTVGDLIAMPATRRELVGHGVSFANSTASYPVCCPSRATFLTGRYAHNHGVMGLYLPT
                     GGYGRFNDLDALPVWLQRAGYRTAHIGKYMNGYGSDRPAIVPPGWTEWYGAVDPTTYR
                     MWDYTLNENGRQVAYGSDPASYQTDVYSRMARDVIRRGSQSGQPFFLSVAFLAPHYEE
                     ASVRSRTGVTVRPAPRHAGRFATLPLPRPRGFNERDRSDKPGFMRRFKALDGNAIARI
                     TAEFRARRESLLAVDEAVDAIVRELSATGTLANTYLLFTSDNGYLQGEHAVPSGKMLP
                     YDPSTRVPLLIRGPGIPARRISQEPVSNIDLAPTILRIAGASAAGGAPIDGRSLLKFA
                     QDPRRRSERLVLHETGGLRATSLQPEEEGSGPIPVRPVRTYRAVRNDRWLYVVYRSGE
                     RELYDLERDPFQLRSRHRDRRYAPTKRALHAELERLATCRGRACRLAGAPIPDPFDRE
                     THTDDDDAELVGVRND"
     gene            complement(2482..>2688)
                     /locus_tag="VHF88_00815"
     CDS             complement(2482..>2688)
                     /locus_tag="VHF88_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160348.1"
                     /translation="QPTAPAQPPRQVGVPPLPVDPTAQIVDPVLDGVGDAVPPAKPVT
                     DGVKGLVDGLTGGLTGGLTGNGGR"
BASE COUNT          341 a          932 c         1051 g          364 t
ORIGIN      
        1 tcgccaacgt cttcgacacc caggtcgccg cggggttcct cgggatgggc aaccaggagg
       61 gctacgagtc gctcgcgcgg cgcgtcctcg gagtcgagct gcgcggcggc gaggggttca
      121 cgcgctggga caagcggccg ctgaccgagc gtcagctcgc ctatgccgcc gacgacgccc
      181 gttgcctgct ggcgctcggc gacgcgatcg cgcgcgagct cgaggctgtc gggaggctcg
      241 agtgggcccg cgaggagtgc cggatcgtcg agcagtcgac cgacgtgcgc tcgccacagc
      301 aggcctacga gcggctgccc aacacgcgcc agctcgacgg cgggcggcgt gccgtcgcct
      361 gggcgctctg cgagtggcgc gacgccgccg cgcgcgagct cgaccgccct ccctcctacc
      421 tgctgcccga ccaggtgctg actcagctcg cccgccgccg gccgaccgac aagcacggtc
      481 tcgagcagat ccgcgggctg ccgcagcaga cgctgcaccg gcggggcggc gaggtgctcg
      541 acgtgatcgc ctcggcggcc cgagcggagc cgccgccacg accgccagcc ggtccgcagc
      601 gtgacccggt cgaccagccg gtcgtgtcgc tcgcccaggc cctgctgcgc catcgcgccc
      661 gcgaggcggg cgtggcgacc gagctgatcg cgacccagtc cgagctaacg gcgctggtgg
      721 cggccgtgcg cgccggagac gccggcgacg gcatccgggc gcttggcggc tggcgccgcg
      781 agctggtagg cgaggagctc tgcgagctcg tcagcggccg cctcgccctc tccgtgggcc
      841 cggacggctc gttagtcgtt gcgcacgccg actagctcgg cgtcgtcgtc atcggtgtgg
      901 gtctcacggt cgaacgggtc gggaatcggc gcgcccgcga gccggcaggc gcggccccgg
      961 catgtggcga gccgctcgag ctcggcgtgg agcgcccgct tggtgggcgc gtagcgacgg
     1021 tcgcggtggc gtgagcggag ctggaacggg tcgcgctcga ggtcgtagag ctcgcgctcc
     1081 ccgctgcgat agacgacgta gagccagcgg tcgttgcgaa cggcgcgata ggtgcgcacg
     1141 gggcgcacgg ggatcgggcc gctgccctcc tcctcaggct gcagcgaggt cgcgcgcagg
     1201 ccgccggtct cgtgcagtac cagccgttcc gaccgccgcc gcgggtcctg ggcgaacttc
     1261 agcagcgagc ggccgtcgat cggcgcgcca ccggccgcgc tagcgccggc gatgcgcagg
     1321 atcgtcggcg cgaggtcgat gttcgagacc ggctcctgac tgatccggcg agcggggatg
     1381 cccgggccgc ggatcagcag cggaaccctg gtcgaagggt cgtagggcag catcttgccg
     1441 ctcgggacgg catgctcacc ctgcaggtag ccgttgtcgg aggtgaacag caggtaggtg
     1501 ttggccagcg tgcccgtcgc gctcagctcg cggacgatgg cgtcgacggc ctcgtcgacg
     1561 gcgagcagcg actcgcggcg cgcgcggaac tcggcggtga tgcgcgcgat cgcgttgccg
     1621 tcgagcgcct tgaagcggcg catgaagccc ggcttgtcgg agcggtcgcg ctcgttgaac
     1681 ccgcgcgggc gcggcaacgg cagcgtcgcg aagcgtccgg cgtggcgcgg cgccggacgg
     1741 acggtcaccc cggtgcggct ccggaccgaa gcctcctcgt agtgcggcgc gaggaacgcg
     1801 accgacagga agaacggctg cccggactgg ctgccgcggc ggatcacgtc gcgcgccatg
     1861 cggctgtaga cgtcggtctg gtagctggcc gggtcgctgc cgtaggccac ctggcggccg
     1921 ttctcgttga gcgtgtagtc ccacatccgg taggtcgtcg gatcgacggc gccgtaccac
     1981 tcggtccagc cgggcgggac gatcgccggg cggtcgctgc catagccgtt catgtacttg
     2041 cctatgtgcg cggtccggta gccggcgcgc tggagccaga cggggagcgc gtcgaggtcg
     2101 ttgaagcgcc catagcctcc ggtcggcagg tagaggccca tcacgccgtg gttgtgcgcg
     2161 taacggccgg tcaagaaggt ggcgcgcgac gggcagcaga ccgggtagga ggccgtcgag
     2221 ttggcgaagc tgacgccgtg gccgacgagc tcgcgccggg tcgccggcat cgcgatcagg
     2281 tcgccgaccg tctggtcgtc ggtcatgatc accaccacgt tcgggcgcgc ggtctgagcc
     2341 gcggcaccgg ccgcgacgac cagcgtgagg gcgagggcga gcgcggcggc agccgcggcc
     2401 gctttcactg caggggccac gttcaccacg gacacgctag agggccgcga gttgcggggc
     2461 gccggctgtc ggtcttcggg cctagcggcc gccgttgccc gtcaggccac cggtgaggcc
     2521 gccggtcagg ccgtcgacga gccccttgac gccgtcggtg accggcttgg cgggcggcac
     2581 cgcgtcgccg acaccgtcga ggacggggtc gacgatctgg gcggtcgggt cgaccggtag
     2641 cggcggcacc cccacctgcc gcggcggctg cgccggcgcg gtcggctg
//
LOCUS       DATABV010000017        12642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2496447, whole genome shotgun sequence.
ACCESSION   DATABV010000017 DATABV010000000
VERSION     DATABV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496447"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VHF88_00820"
     CDS             complement(<1..426)
                     /locus_tag="VHF88_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160349.1"
                     /translation="MTDNWRTAVPVLALLVMLATWGSDPATWVVVVLDVFLAGAIIAA
                     VFHAEVIAERVGEPYGSLALAVAVTVIEVGLIVTLMVAGGDATETLARDTVFAAVMIT
                     CNGIIGISILAAALRDRIAVFNPEGSATAFATVITLATLS"
     gene            628..2196
                     /locus_tag="VHF88_00825"
     CDS             628..2196
                     /locus_tag="VHF88_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2160350.1"
                     /translation="MGIYTYVARARGGPPSSMVLHDIWLAYDDTPAGSWALQVAARLA
                     RRDAARVTVVDAVPPSDEPGDESASLALELLGEREVDHRRRHILHVAENAAPGAAAHV
                     RLVTGSPRRELLDLLRAEPPDLLVAASSSARHWLRRLIEPDVGRMLLRRAPCPVVVVR
                     DVPRPRDRPAEQRHETRIVVDVDMPPPRSDARAAHRDRTRSRPTLLWRIFAANALVLV
                     AATLILIVTPLRISPSPVLSETVSVTLGLVCMLAIHLFLLRRTLEPLRVLTDFMRSID
                     PRHAHRRVPDPPSTADEVSALTHAFNEMLDRLEDERRRSARRALAAQEDERLRIAREL
                     HDQIGQTLTALTIQAERAVEMPDPVDRTLLRQIAGTALQGLDEARSIGRELRPEALDD
                     LGLPNALIVLCRRVASQSHIRITPHFDGDTTDLAPEVELVIYRIAQEAITNAVRHADA
                     ATVEVTLRFDDDTATLTVIDDGRGLPAAPPTDTAGIAGMRERADLIDGAFSIASPATG
                     GTKVRLTVPIEART"
     gene            2193..2852
                     /locus_tag="VHF88_00830"
     CDS             2193..2852
                     /locus_tag="VHF88_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2160351.1"
                     /translation="MTVPLKTRILLADDHTLVRDGLRMILDGQSDLEVVAEAGDGIEA
                     LHLALRENVHLVVLDVAMPRMTGLQVARELQARRPDLRVLMLSVHENEQYFFEALRLG
                     ASGYVLKSGANRDLVEACRSAMRGEPFLYPAAVTALIRDFLDRETAGEPSPQDPLTPR
                     EQDVVKLIAEGHTSDEIADALKISTKTVDRHRSNVLEKLGMRNRVELTRYAIRRGLVE
                     P"
     gene            complement(3065..3137)
                     /locus_tag="VHF88_00835"
     tRNA            complement(3065..3137)
                     /locus_tag="VHF88_00835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3102..3104),aa:Val,seq:gac)
     gene            3238..3864
                     /locus_tag="VHF88_00840"
     CDS             3238..3864
                     /locus_tag="VHF88_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX2160352.1"
                     /translation="MRLSSVLAPRDREFFDLFEEAGGNIVRAADLLDRMLRAFPDRGD
                     DLARDILICEQDGDRITHDIIRRLNETFVTPIDREDIYELASALDDIVDLTEEVADYL
                     ILYKIEAPMEQAQRLAHVLLQCSRQIAEAMPRLRGFKDLSHYTVEINRLENDGDRIVR
                     EAIASLFDNGIDPMVVIRWKDIYERLEEAIDASEKVANILEGIVIKNS"
     gene            3885..4988
                     /locus_tag="VHF88_00845"
     CDS             3885..4988
                     /locus_tag="VHF88_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX2160353.1"
                     /translation="MDSEIILVIVVVTALAFDFTNGFHDTANVVATSISTRAMPPKVA
                     VGYAAVLNFFGAFLSLEVAATIAGGIVEADLITPLIVFGGLIGAIAWNLVTWYFGLPS
                     SSSHALIGGVVGAAFVAAGPDAVLGDGLLGKVAVPAILAPTLAFAVAGIGILVIYRIV
                     GRQHPGPVTRGFRFGQIVSGGLLALAHGTNDAQKTMGVITLALIAGGELGAGADPPFW
                     VIVSAATAIALGTYMGGWRIIKTMGSRIIKMDSAQGFAAQGSGAAVILASTHVGFPLS
                     TTHTISGAVMGAGAAKRVSAVRWGVAGNIVVAWILTLPAAAGIGALTYGLTRIFGTGA
                     AGPLVVSAILLVTMAVVFARRLRQGSPITAAEG"
     gene            4992..5237
                     /locus_tag="VHF88_00850"
     CDS             4992..5237
                     /locus_tag="VHF88_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160354.1"
                     /translation="MTPLADIVDVSDLVSVVWTSLVAGIGVCLVFSLAIVGFARATDM
                     RREGNGVAMVAYTTLALIAFVAVMALVVFGVVVMTSK"
     gene            complement(5221..6312)
                     /locus_tag="VHF88_00855"
     CDS             complement(5221..6312)
                     /locus_tag="VHF88_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2160355.1"
                     /translation="MDLAMLRAQFPVLERLAYLNAGSVGPAARSAVGAAEVDMQRQLE
                     EGRGARVQFDHAAEQGDRLRARVAALLGADPWELALTGATTDGVNAVLAGLDLEPGDE
                     ILTSDEEHPGILAPLGLVVERRGVKVRTAPFDAIADHVGPQTRLIACSHVSWISGRVV
                     DGEALATAPVPVLLDGAQAAGAIPVDVHELGCEYYAASGQKWLCGPVGSGYLYVREDA
                     IEALSPMSPSYGTLADPLRALELPLVPGAARFDGGFPVTHQTAWALGALDVLENAGID
                     AVQARAAELAATLTERLAERGVAVAERGPSTLVSWEAADPQAEVGRLLDAGLLVRNLP
                     GTPYVRASVGGWTSDEEIERLVAAVTSTS"
     gene            6349..7101
                     /locus_tag="VHF88_00860"
     CDS             6349..7101
                     /locus_tag="VHF88_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2160356.1"
                     /translation="MPVTIERSLHPGFLSNAYLVYDEPGGTAVIIDSGAPPEPLLESI
                     ERNEVTPTHLLLTHHHHDHVERNATYKERFGVEILAHPLEAARLEDVDRTIEPGDLVL
                     ETGGLRIGALHTPGHTDGMLNFVVGGEDVFTGDTLFKGSVGGVKAPGATTFADLRSSV
                     MDVLMALPPQTRLHPGHTDPTTVAEEWGSNPFVRVWRGLDPEGDDRCTVWERGATLVL
                     WAPDYDGGHKAWIRWDDTGEDDIVPGSQVVRD"
     gene            complement(7106..7549)
                     /locus_tag="VHF88_00865"
     CDS             complement(7106..7549)
                     /locus_tag="VHF88_00865"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX2160357.1"
                     /translation="MRKLALLTTLCAAAAVPAIAFAADGSVVSSAATTGPTLVVHDLG
                     DPLQVQEARRQLRIAKRRARPPRPEPVAVPAHLQAIAQCESGGDPTAIGGGGLYRGKY
                     QFSVATWQAVGGTGDPAAAPETEQDRRAIILYNTSGPGQWPVCGS"
     gene            7839..8306
                     /locus_tag="VHF88_00870"
     CDS             7839..8306
                     /locus_tag="VHF88_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160358.1"
                     /translation="MAAAAGAVAIALGALFAGTAGAQAAADLSKLDVAGPKTVKQGAS
                     KTYRVTVVNVGDAAAEGIEVVASGGGKGRTSVSDTLAPAASTVVRVKVKITGKAGKTV
                     TVTFKATTSAGTTASGKIRVTVPKKKTKKRGPAGAPQIPLYPAPQLPSVGGGS"
     gene            8311..9267
                     /locus_tag="VHF88_00875"
     CDS             8311..9267
                     /locus_tag="VHF88_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160359.1"
                     /translation="MRKHRRVATGCRRAVVTVALCGGALLAWSAPADAARPASGAERL
                     SMLRAAAASEGGAMVRLRVGSGGRATMEPLGSRGPATILAVRSSLDRSWALLIASLNG
                     RADVRRTFLLERRHGSWRVRMSAGRGDEGDGLCRLRRPGIAVALDLGLSTDTWSKTCR
                     HKRERKRLVRRMSAAELASVRAMVEWKYDQSTFELRPGPVQPAVHEVFASDCAWDGRG
                     SVVPRPVGEVARSDPRWGIVRISCVIGSDGFGALESATVMLVARAGDSGPFTRVPAHT
                     HPAWSVRGGLCRADRRWPVPASARVALEFCTPFPAALRPALR"
     gene            9336..11255
                     /gene="thrS"
                     /locus_tag="VHF88_00880"
     CDS             9336..11255
                     /gene="thrS"
                     /locus_tag="VHF88_00880"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX2160360.1"
                     /translation="MTVQLPDASELDVPDGASGLDVALAIGEGLARAALGIKVDGELR
                     DLSTPVPDGAQVEIVTAKSGADALWLIRHDAAHVLATAVTELYPGVKVSIGPPIEDGF
                     YYDFEFPEGVKLSDADFERIEQKMREHVKADEAFERSELPVGEAIERFRTEGQDYKVE
                     LIEDLVRDQQVETVSLYRNGPFTDLCRGPHAPSTKRIKAFKLTSVAGAYWRGDSSRQM
                     LTRVYGTAFLSKEDLARHLERLEEARKRDHRKLGKELDLFMFSELSPGSPFWKPAGQQ
                     LWNELTDLWRGENAERGYREVHTPILYDAELWKQSGHWHVYRENMYFTEVEGRTMGLK
                     PMNCPAHTQIYSSERRSYRDLPIRYSEAGLVHRHEPSGTLHGLLRVRHITQDDAHIFC
                     TEEQVPEEVQRCLDFGFAIYDVFGFEPRLELSTRPEKRVGSEQMWDRAEAALAKALDD
                     RGLDYELNPGDGAFYGPKIDLHMTDSIGRSWQLGTVQLDYYMPEQFELAYTGADNAEH
                     RPVMIHRALMGSFERFIGILIEHYAGDFPLWLAPVQALVLPISDRHNDHAGRVAAELR
                     ASGLRVEVDDRAESVGRKIRDGELRKAPYMLILGDKEAEQGNVAVRRHGEGDLGALTV
                     AEFAERAAAEVSSRR"
     gene            11379..11963
                     /gene="infC"
                     /locus_tag="VHF88_00885"
     CDS             11379..11963
                     /gene="infC"
                     /locus_tag="VHF88_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX2160361.1"
                     /translation="MPVPRRFDRRPPDRDDTRVNERIRVREVRLIGEDGEQVGVLPID
                     RALAYARERDLDLVEVAADARPPVCRVLDYSKYKYEQEQKAKAARKHQKQVNVREIKL
                     RPKIADNDYETKKSHVVRFLEGDDRVKVTIMFRGREQTHPERGVALLMRLAEDVADLG
                     VIDQRPIQDGRNMTMLLSPVAKKPAEDGDQAQSA"
     gene            11978..12172
                     /gene="rpmI"
                     /locus_tag="VHF88_00890"
     CDS             11978..12172
                     /gene="rpmI"
                     /locus_tag="VHF88_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX2160362.1"
                     /translation="MPKMKTHSGAKKRFRKTGSGKLKARHGMTSHILEKKSAKRKRKL
                     GRPVEIAKADEARVKGLLRG"
     gene            12169..12528
                     /gene="rplT"
                     /locus_tag="VHF88_00895"
     CDS             12169..12528
                     /gene="rplT"
                     /locus_tag="VHF88_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX2160363.1"
                     /translation="MSRVKRSLHAKKKRRATLERTKGYRGQANSSYKRAKEALLKADS
                     YAYRDRRNKKREFRRLWIQRINAAARREGMTYGTFMHGLKLAGVELDRKVLADIAVRD
                     PETFRRFADTAREAAAA"
BASE COUNT         1956 a         4590 c         4301 g         1795 t
ORIGIN      
        1 gctgagcgtc gccagggtga tgaccgtcgc gaaggccgtg gccgagccct cggggttgaa
       61 gacggcgatc cggtcgcgca gagccgcggc gaggatcgag atgccgatga tgccgttgca
      121 ggtgatcatc acggcggcga agacggtgtc gcgcgcgagc gtctcggtgg cgtcgccacc
      181 cgccaccatc aacgtgacga tcagccccac ctcgatcacc gtcacggcca ctgccaacgc
      241 cagcgagccg tagggctcgc cgacccgctc ggcgatcacc tcggcgtgga acaccgcagc
      301 gatgatggcc ccggcgagga agacgtcgag caccacgacc acccaggtag cggggtcgct
      361 gccccaggtc gccagcatca ccagcagcgc cagcacaggg accgcggtcc gccagttgtc
      421 ggtcaagcgc gtggagatcg tcgcgatcct acgtcgcccc ctgaggtcgg tgctcgtcaa
      481 gactcaccca ccgctacggg gattgtccag tggccgccaa ggggcagcgg ccaccggacc
      541 tacgacggta cgcgcgaacc gagcgcccgc atatgggtga cgaccctcaa gcgcgcagcg
      601 aaccccgcgg cccgcccatc catgggaatg ggcatctaca cctatgtcgc acgggcgcgt
      661 ggcggtccac catcgagcat ggtgctgcac gacatctggt tggcatacga cgacacaccg
      721 gccggtagct gggcgctcca ggtcgccgcc cgcctggccc ggcgcgacgc cgccagggtc
      781 accgtcgtcg acgccgttcc tccctcggac gagccaggcg acgaaagcgc atcgctggcg
      841 ctcgagctgc tcggcgagcg cgaggtagat caccggcgac gccacatcct gcacgtcgcc
      901 gagaacgcgg cgcccggtgc cgccgcccac gtccgtctcg tcacgggatc tcctcgccga
      961 gagctgctcg acctgctccg ggcagagcct cctgaccttc tcgtagcggc gtccagctca
     1021 gcgcgtcact ggctcagacg tctcatcgag ccggacgtcg gccggatgct cctgcgccgg
     1081 gctccctgtc cggtggtggt cgtccgagac gtcccccgtc cgcgtgaccg cccggctgag
     1141 cagcggcacg agacccgtat cgtcgtcgac gtcgacatgc cgccgccacg atcagacgcg
     1201 cgagccgccc accgcgacag gacgcgttcc cgtccgacgc tgctgtggcg gatcttcgcc
     1261 gccaacgcgc tcgtgcttgt cgccgcgacg ctgatcctga tcgtcacgcc gctgcggatc
     1321 agccccagcc cggtgctgtc ggagaccgtc agcgtcacgc tcggactggt ctgcatgctg
     1381 gcgatccacc tgttcctgct gcggcgaacg ctcgagccgc tgcgtgtcct gaccgacttc
     1441 atgaggtcga tcgacccgcg acacgcccac cggcgcgtgc ccgatccgcc ctcgaccgcc
     1501 gacgaggtga gcgcgctcac ccacgccttc aacgagatgc tcgaccgctt ggaggacgag
     1561 cggcgccgca gcgcgaggcg cgcgctcgcc gcccaagagg acgagcggct gcgcatcgcg
     1621 cgcgagctgc acgaccagat cggccagacg ctcacggccc tgaccatcca ggccgagcgc
     1681 gccgtcgaga tgcccgatcc tgtggaccga acgttgctgc gccagatcgc cgggaccgcg
     1741 ctccaaggcc tcgacgaggc ccggagcatc ggccgcgagc tgcgtcccga ggcgctcgac
     1801 gacctcggcc tccccaacgc gctgatcgtc ctctgccgac gcgtggcctc tcagagccac
     1861 atacggatca ctccgcactt cgacggcgac acgacggatc tcgccccaga ggtcgagctg
     1921 gtgatctacc gcatcgccca ggaggcgatc accaacgccg tccgccacgc cgacgcggcc
     1981 acggtcgagg tgacgctgcg cttcgacgac gacacagcca cgctgaccgt catcgacgac
     2041 ggacgcggcc tgcccgccgc gccgccgacc gacaccgccg gcatcgccgg gatgcgcgag
     2101 cgcgcggacc tgatcgacgg cgccttctcg atcgcctcgc cggcgaccgg aggcaccaag
     2161 gtccgcctaa ccgtcccgat cgaggcacgc acgtgaccgt cccgctcaag acgcgcatcc
     2221 tgttggccga cgaccacacg ctcgtgcgcg acggcttgcg catgatcctc gacggccaat
     2281 ccgacctgga ggtcgtcgcc gaggccgggg acggcatcga agccctccac ctcgctctgc
     2341 gcgaaaacgt ccatctcgtc gtgctcgacg tcgccatgcc acgcatgacc ggcctccaag
     2401 tcgcccgcga actgcaagca cgccgccccg acttgcgcgt cctgatgctc tccgtccacg
     2461 agaacgagca gtacttcttc gaggccctgc gcctcggggc atccggctac gtcctcaagt
     2521 ccggcgccaa ccgcgacctc gtcgaggcct gccgctccgc gatgcgcggc gagccgttcc
     2581 tctacccggc cgccgtcacc gccctgatcc gcgacttcct cgaccgcgag accgccggcg
     2641 agccatcacc acaagatccc ctcacgccac gcgagcagga cgtcgtcaag ctcatcgccg
     2701 agggacacac cagcgacgag atcgccgacg cgctcaagat ctcgacgaag accgtcgacc
     2761 gccaccgctc caacgtcctc gagaagctcg gcatgcgcaa ccgcgtcgag ctcacccgct
     2821 acgccatccg acgcggcctc gtcgagccct gaccgccacc ggcctcgcac ggaaacggcg
     2881 ctgagagtgc tcgctcggag tcgttcgcgc tttgagccga gacgagcgcc agcggttagc
     2941 tgatcttcag gacgctgtcg aggccatcct cgctcactcg acgcgcgcgc tgctcttcca
     3001 gttcgtggtc gtcggcgagg ccccgcgata gccaggatgc caacgcattc aggaggtttc
     3061 gaagtgggcc gtgctgggct cgaaccagcg acctccgcct tgtcgaggcg gcgctctccc
     3121 aactgagcta acggcccttc gcggccgccg aggatagcgg gacggagcgg gcgccggcca
     3181 gccgccgcgc gcgggctggg tgggcatcct tggcaactgg tagcttggcc gcgccgcatg
     3241 cgtctctcca gcgtcctcgc cccccgtgac cgtgagttct tcgatctgtt cgaggaggcc
     3301 ggcggcaaca tcgtgcgcgc cgcggacctg ctcgaccgga tgctgcgcgc gttccccgac
     3361 cgcggcgacg acctggcgcg cgacatcctg atctgcgagc aggacggtga ccggatcacc
     3421 cacgacatca tccggcggct gaacgagacg ttcgtcacgc cgatcgaccg cgaggacatc
     3481 tacgagctcg cgtcagcgct cgacgacatc gtcgacctca ccgaggaggt cgccgactac
     3541 ctgatcctct acaagatcga ggcgccgatg gagcaggcgc agcggctcgc ccacgtgctg
     3601 ctgcagtgct cacgccagat cgccgaggcg atgccgcggc tgcgcggctt caaggacctc
     3661 tcgcactaca cggtcgagat caaccggctc gagaacgacg gcgacaggat cgtccgcgag
     3721 gcgatcgcgt cgctgttcga caacgggatc gacccgatgg tcgtgatccg ctggaaggac
     3781 atctacgagc gcctcgagga ggcgatcgac gcaagcgaga aggtcgcgaa catcctcgag
     3841 ggcatcgtca tcaagaactc gtgacgcagc actagcgcca ccgcatggac agcgagatca
     3901 tcctggtcat cgtcgtggtg acggcgctgg cgttcgactt caccaacggc ttccacgaca
     3961 ccgccaacgt cgtcgcgacg tcgatctcga cgcgcgccat gccgccgaag gtcgccgtcg
     4021 gctacgcggc ggtcctcaac ttcttcggtg cttttctctc gctcgaggtg gccgcgacga
     4081 tcgccggcgg gatcgtcgag gcggacctca tcacgccgct gatcgtcttc ggggggctga
     4141 tcggggcgat cgcatggaac ctcgtgacct ggtacttcgg cctgccgtcg agctcctcgc
     4201 acgcgctgat cggcggcgtc gtcggagcgg ccttcgtcgc cgccggcccg gacgccgtcc
     4261 tcggcgacgg gctgctgggc aaggtcgcgg tcccggcgat tctggcgccg acgctcgcgt
     4321 tcgccgtcgc ggggatcggc atcctggtca tctaccggat cgtcggccgc cagcatccgg
     4381 gacctgtcac gcgcggcttc cgcttcggcc agatcgtctc gggcggcctg ctcgcgctcg
     4441 cgcacggcac caacgacgcc cagaagacga tgggcgtgat cacgctggcg ctgatcgccg
     4501 gcggtgagct cggtgcgggt gccgacccgc ctttctgggt gatcgtctcg gcggccacgg
     4561 cgatcgcgct cggcacgtac atgggcggct ggcggatcat caagacgatg ggcagccgga
     4621 tcatcaagat ggactccgcg caggggttcg ccgcccaggg cagcggggcg gcggtgatcc
     4681 tcgcctcgac ccacgtcggc ttcccgctct cgacgacgca cacgatctcc ggcgctgtga
     4741 tgggcgccgg tgccgccaag cgcgtgtccg cggtccgctg gggcgtcgcg ggcaacatcg
     4801 tcgtcgcctg gatcctcacg ctgccggccg ccgctgggat cggcgcgttg acctacggac
     4861 tcacgcggat cttcggcacg ggcgccgccg gcccgctggt cgtctcggcg atcctgctcg
     4921 tgacgatggc ggtcgtgttc gcccgccgcc tgcgtcaggg ctcgccaatc acggccgcgg
     4981 agggctaggc attgacgccg ctcgcggaca tcgtggacgt cagcgacctg gtcagcgtcg
     5041 tctggacgtc gctggtcgcc gggatcggcg tctgcctcgt cttctcgctg gcgatcgtcg
     5101 gcttcgcacg ggcgaccgac atgcgccgcg agggcaacgg cgtcgcgatg gtcgcctaca
     5161 ctacgttggc actgatcgcc ttcgtcgcgg tgatggcact ggtcgtcttc ggcgtcgtcg
     5221 tcatgacgtc gaagtgaccg cggcgaccag ccgctcgatc tcctcgtcgc tcgtccagcc
     5281 gccgaccgac gcgcgcacgt agggcgttcc cggcaggttg cgtacgagca ggccggcgtc
     5341 gagcagccgc ccgacctcgg cctggggatc ggccgcctcc caggagacga gcgtcgaagg
     5401 cccgcgctcg gcgaccgcca cgccccgctc cgcgagccgc tcggtcaggg tcgcggccag
     5461 ctccgccgcg cgcgcctgga ccgcgtcgat ccccgcgttc tccagcacgt cgagcgcccc
     5521 gagcgcccat gccgtctggt gcgtgaccgg gaagccgccg tcgaagcggg cggcgcccgg
     5581 cacgagcggc agctcgagcg cgcgcagcgg gtcggcgagc gtgccgtagc tcggcgacat
     5641 cggggagagc gcctcgatcg cgtcctcgcg gacgtagagg tagccgctgc cgaccggccc
     5701 gcagagccac ttctgacccg acgccgcgta gtactcgcag ccgagctcgt ggacgtcgac
     5761 cgggatcgcg cctgcggcct gggcgccgtc gagcaggacg gggacggggg ccgtcgcgag
     5821 cgcctcgcca tcgacgacgc ggccgctgat ccacgacacg tgcgagcagg cgatcagccg
     5881 cgtctgcggg ccgacgtggt cggcgatcgc atcgaacggc gcggtccgga ccttgacgcc
     5941 ccgccgctcg acgaccagtc cgagcggcgc gagaattcca gggtgctcct cgtcgctagt
     6001 caggatctcg tcgcccggct ccagatcgag cccggccagc accgcgttga cgccgtcggt
     6061 cgtcgcgccg gtcagcgcga gctcccacgg gtcggcgccg agcagcgccg cgacccgcgc
     6121 gcgcaggcgg tcgccctgct cggccgcgtg gtcgaactgg acccgagcgc cacggccctc
     6181 ctcgagctgg cgctgcatgt cgacctcggc ggcgccgacc gccgagcgcg ccgccggccc
     6241 gaccgacccc gcgttgaggt aggcgagccg ttcgagcacc gggaactgcg ctcgaagcat
     6301 cgcgaggtcc atgcggtctg atgctacgcg ccggataggc tccgcccgat gcccgtcacg
     6361 atcgagcgtt cgctgcaccc cggcttcctg tcgaacgcct acctcgtcta cgacgagccc
     6421 ggcgggacgg cggtgatcat cgactcgggt gccccgcccg agccgctgct cgagtcgatc
     6481 gagcgcaacg aggtcacgcc gactcacctg ctgctcaccc atcaccatca cgaccacgtc
     6541 gagcgcaacg cgacctacaa ggagcgcttc ggcgtcgaga tcctcgcgca cccgctcgag
     6601 gcggcgcggc tcgaggacgt cgatcggacg atcgagcccg gcgacctggt gctcgagacc
     6661 ggcggcctgc ggatcggcgc gctgcacacc cccggccaca ccgacgggat gctcaacttc
     6721 gtcgtcggcg gcgaggacgt cttcaccggc gacacgctct tcaagggctc ggtcggcggc
     6781 gtcaaggcgc cgggggcgac caccttcgcc gacctgaggt catcggtgat ggacgtgctg
     6841 atggcgctgc cgccgcagac gcgcctgcat cccggccaca ccgatccgac caccgtcgcc
     6901 gaggagtggg ggtccaaccc cttcgtgcgt gtctggcgcg gcctcgaccc cgagggcgac
     6961 gaccgctgca cggtctggga gcgcggcgcg acgctcgtcc tctgggcgcc cgactacgac
     7021 ggcggccaca aggcctggat ccgctgggac gacacaggcg aggacgacat tgtccccggt
     7081 tctcaggtcg tcagggacta gcgccctaac tgccgcagac cggccactgg cccgggccgg
     7141 aggtgttgta gaggatgatc gcgcggcggt cctgctcggt ctccggcgcg gcggccgggt
     7201 cgccggtccc gccgaccgcc tgccaggtgg cgaccgagaa ctggtacttg ccgcggtaga
     7261 gcccgccgcc gccgatcgcg gtcggatcgc cgcccgactc gcactgcgcg atcgcctgca
     7321 ggtgggctgg cacagcgacc ggctccggcc tcggcgggcg cgcccgccgc ttggcgatcc
     7381 gcagctggcg tcgcgcctcc tgaacctgga gcgggtcgcc gaggtcgtgg acgacgagcg
     7441 tcggtccggt ggtcgcggcc gacgacacga cggagccgtc cgcggcgaac gcgatcgccg
     7501 gaacggcggc cgcggcacag agggttgtca gtagggcgag cttgcgcata tggcgtggac
     7561 ctccacgctt cggggtcagg cgccggatgg accggcctcc cgcgctcttg tgcgtcgctg
     7621 cgacgtgcgc gggaagtgga gccggcgggg acggggtccg cgcgagaccg gctcgactcg
     7681 ccagggtagg cgccatccgg cacccgtctg tgacgagcgt cacactgtcc ctttcgacgg
     7741 ctcgaccctc cgcgcgatat ttgcgcgctc gtcaggcacg ggttaggttc gccttccgaa
     7801 gtactggccc tgatccaagg agacccgtga gcagaccaat ggcagcagcg gccggcgcgg
     7861 ttgcgatcgc gcttggagcc ctcttcgcgg gtaccgcggg cgcgcaggcg gccgctgatc
     7921 tctccaagct cgacgtcgcg gggccgaaga cggtcaagca gggggcgtcc aagacctacc
     7981 gcgtgaccgt cgtcaacgtg ggcgacgcgg ccgccgaggg catcgaagtc gtcgcaagcg
     8041 gaggcggcaa aggtcgcacc agcgtcagcg acacgctcgc tcccgccgcg tcgacggtcg
     8101 tgcgcgtgaa ggtcaagatc accggcaagg caggcaagac cgtcaccgtg acgttcaagg
     8161 cgaccacgtc agccggaacg acggcgtccg gcaagatcag ggtcacggtg ccgaagaaga
     8221 agacgaagaa gcggggaccc gccggcgccc ctcagatccc gctgtatccg gcgccgcagc
     8281 tgccgagcgt cggcggcggc agctagcgcg gtgcgcaagc accgtcgcgt cgcgaccggc
     8341 tgccgacgag ccgtcgtcac ggtcgcgctg tgcggcggcg cgctgctcgc ctggagcgct
     8401 cccgctgacg ctgcgcgccc ggcgagcggc gccgagcggc tgtcgatgct gcgcgccgca
     8461 gccgcgtcag agggcggggc gatggtgcgc ctgcgcgtcg gtagtggggg gcgggcgacg
     8521 atggagccgc tcggctcccg cggaccggcg acgatcctcg ccgtgcgctc gtcgctcgac
     8581 cgctcctggg cgctgttgat cgcctcgctg aacggccgcg cggacgtccg gcgaaccttc
     8641 ctgctcgagc gtcgccacgg aagctggcgg gtgcgcatgt cggctggccg cggcgacgag
     8701 ggcgacgggc tctgccgcct gcggcggccg ggtatcgcgg tggctctcga tctcggactg
     8761 agcacagaca cctggtcgaa gacgtgtcgc cacaagcgtg agcgcaagcg gctggtacgc
     8821 cggatgtcgg cggccgagct ggcgtctgta cgtgcgatgg tcgagtggaa gtacgaccag
     8881 agcacgttcg agctgcgccc gggaccggtc cagccagcgg tccatgaggt cttcgcgtcc
     8941 gattgcgcgt gggacgggcg cgggagcgtg gtgccgcggc cggtcgggga ggtcgctcgc
     9001 tccgatccgc gctggggaat cgtccggatc agttgcgtga tcggtagcga cggctttggg
     9061 gcgctggagt cggcgacggt gatgctcgtc gcgcgcgctg gcgactcggg accgttcacg
     9121 cgcgtaccgg cgcacaccca cccggcctgg agcgtccgcg gcgggctgtg ccgcgccgat
     9181 cggcgctggc cggtcccggc gtctgcgcgc gtcgcgctcg agttctgcac gccgttcccc
     9241 gctgcgcttc gcccggcgct gcgctgaacg acaccgtgaa cggataggcc gtgacgagcg
     9301 tcacggctcg acggcgcgtg taggttccag gccgcatgac ggttcagctc ccagacgcgt
     9361 ccgagctcga cgttcccgac ggcgccagcg gcctcgacgt cgcgctcgcg atcggcgagg
     9421 gactggcgcg cgcggcgctc ggaatcaagg tcgacggcga gctgcgcgac ctttcgacgc
     9481 ctgtgccgga cggcgcccaa gtcgagatcg tcactgcgaa gagcggcgcg gacgcgctct
     9541 ggctgatccg ccacgacgcc gcccacgtcc tcgcgaccgc ggtgaccgag ctctacccgg
     9601 gggtgaaggt ctcgatcggg ccgccgatcg aggatggctt ctactacgac ttcgagttcc
     9661 ccgagggggt caagctctcc gacgccgact tcgagcgcat cgagcagaag atgcgcgagc
     9721 acgtcaaggc cgacgaggcc ttcgagcgca gcgagttgcc cgtcggcgag gcgatcgagc
     9781 gcttccgcac cgagggacag gactacaagg tcgagctgat cgaggacctc gtccgcgatc
     9841 agcaggtcga gaccgtctcg ctctaccgca acggcccctt caccgacctc tgccgcggcc
     9901 cgcacgcgcc gtcgacgaag cggatcaagg ccttcaagct gaccagcgtc gccggcgcct
     9961 actggcgcgg cgactccagc cgccagatgc tgacgcgcgt ctacggcacg gcgttcctat
    10021 ccaaggagga cctcgcccgg cacctcgagc ggctcgagga ggcgcgcaag cgcgaccacc
    10081 gcaagctcgg caaggagctc gacctgttca tgttcagcga gttgtcgccc ggctcgccgt
    10141 tctggaagcc ggccgggcag caactgtgga acgagctgac cgacctctgg cgcggcgaga
    10201 acgccgagcg cggctaccgc gaggtccaca cgccgatcct ctacgacgcc gagctctgga
    10261 agcagtcggg ccactggcac gtctaccgcg agaacatgta cttcaccgag gtcgagggcc
    10321 gcacgatggg cctgaagccg atgaactgcc cggcgcacac ccagatctac tcttccgagc
    10381 gccgctccta ccgcgacctg ccgatccgct actccgaggc cggcctcgtc caccgccacg
    10441 agccgagcgg gacgctgcac ggcctgctgc gcgtccgcca catcacccag gacgacgccc
    10501 acatcttctg caccgaggag caggtccccg aggaggtcca gcgctgcctc gacttcggct
    10561 tcgcgatcta cgacgtcttc ggcttcgagc cacgactcga gctgtcgacc cggcccgaga
    10621 agcgcgtcgg cagcgagcag atgtgggacc gcgccgaggc ggcgctcgcc aaggcgctcg
    10681 acgaccgcgg cctcgactac gagctcaacc cgggcgacgg cgccttctac ggcccgaaga
    10741 tcgacctcca catgaccgac tcgatcggcc gctcatggca gctcggcacc gtccagctcg
    10801 actactacat gcccgagcag ttcgagctcg cctacaccgg cgccgacaac gccgagcacc
    10861 gcccggtgat gatccaccgt gcgctgatgg gtagcttcga gcgcttcatc ggcatcctga
    10921 tcgagcacta cgccggcgac ttcccgctct ggctggcgcc ggtccaggcg ctggtgctgc
    10981 cgatctccga ccgtcacaac gaccacgccg gccgcgtcgc ggccgagctg cgtgcgagcg
    11041 gcctgcgcgt cgaggtcgac gaccgcgccg agtcggtcgg ccgcaagatc cgcgatggcg
    11101 agctgcgcaa ggcgccctac atgctgatcc tcggcgacaa ggaggccgag caaggcaacg
    11161 tcgcggtccg ccgccacggc gagggcgacc tcggcgcgct gaccgtcgcc gagttcgcgg
    11221 agcgcgccgc cgcagaggtc tcctcgcgtc gctgagccgg cgctatactc gccgacgaat
    11281 gacgggcacc acgaccacgc gcacagcttg ccgttgacgc ttcgtgcccg ctcccgactc
    11341 cctctctcgc ctttgacgtc tagccgggcc ttcgcgtagt gccagttccg cgcaggtttg
    11401 accggcgtcc gcccgaccgc gacgacacgc gcgtcaatga gcgcatccgg gttcgcgagg
    11461 tgcgcctgat cggcgaggac ggcgagcagg tcggcgtgct gccgatcgac agggcgctcg
    11521 cctacgcccg cgagcgcgat ctcgacctcg tcgaggtggc ggccgacgcg cgcccgccgg
    11581 tctgccgcgt gctcgactac tcgaagtaca agtacgagca ggagcagaag gccaaggccg
    11641 cgcgcaagca ccagaagcag gtcaacgtgc gcgagatcaa gctgcggccg aagatcgcgg
    11701 acaacgacta cgagaccaag aagagccacg tggtccgctt cctcgaggga gacgaccgcg
    11761 tcaaggtcac aatcatgttc cgcggccgcg agcagacgca ccccgagcgc ggggtcgcgc
    11821 tgctgatgcg gctcgccgag gacgtcgccg atctcggcgt gatcgaccag cgtccgatcc
    11881 aggacggccg caacatgacg atgttgctgt cgccggtggc gaagaagccc gccgaggacg
    11941 gcgatcaagc gcagtctgct tgaatacccc gttcgccatg cccaagatga agacgcactc
    12001 cggcgctaag aagcgcttcc gcaagaccgg cagcggcaag ctgaaggcgc gtcacggcat
    12061 gaccagccac atcctcgaga agaagagcgc caagcgcaag cgcaagctcg gccggccggt
    12121 cgagatcgcg aaggccgacg aggcgcgcgt caaggggctg ttgcgcggat gagccgagtc
    12181 aagcgctcac tgcacgcgaa gaagaagcgt cgggcgacgc tcgagcggac caagggttac
    12241 cgcggtcagg cgaactcgag ctacaagcgc gccaaggagg cgctgctcaa ggccgacagc
    12301 tacgcctacc gcgaccggcg caacaagaag cgcgagttcc gtcgtctctg gatccagcgg
    12361 atcaacgccg ccgcgcggcg cgagggcatg acctacggca cgttcatgca cggcctcaag
    12421 ttggccggcg tcgagctcga ccgcaaggtg ctcgccgaca tcgccgtgcg cgaccccgag
    12481 acgttccgac gctttgccga caccgcccgg gaggcggccg ctgcctgact ccaggtcagc
    12541 ggtcgaacga gcttcccggg gcgcccagag tggcgccctt tctcgttcca ggaccggttt
    12601 ccgtgatcac atccagagac aacagccagc tcaagcagat cc
//
LOCUS       DATABV010000018         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2737286, whole genome shotgun sequence.
ACCESSION   DATABV010000018 DATABV010000000
VERSION     DATABV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2737286"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHF88_00900"
     CDS             <1..355
                     /locus_tag="VHF88_00900"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX2160364.1"
                     /translation="RLIASELQKAVKDGGDDEVAVLQRERKRRLESAAAYRDGGRDDL
                     ATAEESEAELIAAYMPEQLDDTELARIVAAAVAESGASSPREMGDVMKLVMPQVRGRA
                     DGKRVSAAVKEKLTA"
     gene            355..1335
                     /locus_tag="VHF88_00905"
     CDS             355..1335
                     /locus_tag="VHF88_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX2160365.1"
                     /translation="MRRQIELSNDVAAELAGPEDLIIRTLEGHLDCEVFLRGNVLTLD
                     GSEDQIEMGATVVRELSDLIRQGHEVAPGTIEAIGSALDEHQSPSEILEDVVWRHRGL
                     KVAPKTVNQKRYVDAIRTNTIAVGIGPAGTGKSFLAVAMAVAALQRREVNRIVLTRPA
                     VEAGERLGFLPGDLMAKVDPYLRPLFDALYDMLEPEKVNQHLERGVIEVAPLAFMRGR
                     TLNDSFIIVDEAQNTTPEQMKMVMTRLGFGSKMVVTGDVTQVDLPRETHSGLVVIGDI
                     LEDVEGIEFVRFGGEDVVRHKLVQRIVAAYSEYAESQAPELRKSQSRRRA"
     gene            1350..1712
                     /gene="ybeY"
                     /locus_tag="VHF88_00910"
     CDS             1350..1712
                     /gene="ybeY"
                     /locus_tag="VHF88_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX2160366.1"
                     /translation="MDAPAAPAGFDAAARAALAAAGVDGELHVAIAFVGAAEIHALNR
                     QHRGRDEPTDVLSFPVDGDGPAPGPRELGDVVICPEHTDDLAEAVVHGVLHLCGYDHE
                     TDDGEMLALQDRVMERLR"
     gene            1709..2596
                     /gene="era"
                     /locus_tag="VHF88_00915"
     CDS             1709..2596
                     /gene="era"
                     /locus_tag="VHF88_00915"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX2160367.1"
                     /translation="MSATRAGFVALAGRPNAGKSTLLNRIVGDKVAIVSDKPQTTRRE
                     IRGIATGDDWQLVLVDLPGVQRPRDPMTERMQRRVEQALRDADAVLFMLNGAQRIGPG
                     DRYIARAIRSSGVPAVTAVNKIDKLGRAATAAALTGAADLDVQGDIFPISAKSGDGVE
                     ALVEHVVGLLPEGPWLYPPEDRSDQAEDVRIAELVREQVLRRTREELPHAVEVEVTGI
                     EERGDGLLEIEASVWAESESQKGILIGGRGAMVKAIGTAARREIESVLDRRVHLELRV
                     RVRRGWRADDALLDRLGID"
     gene            2723..3322
                     /gene="hisIE"
                     /locus_tag="VHF88_00920"
     CDS             2723..3322
                     /gene="hisIE"
                     /locus_tag="VHF88_00920"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEX2160368.1"
                     /translation="MEEIRFDDAGLVPCVMQDARTGEVLTLAYMNEEALRLTRETGEV
                     HFWSRSRGQIWHKGATSGNTMKVRALRVDCDADAVVALVDPSGPACHTGARSCFHSGD
                     LDPQAGEALAELERTIAARKAAGDEGSSYTAQLLADPPLIGEKVREEAEEVTRAAREE
                     SDRRVREEAADVLYHLAVLLASRDMSYADAFEELNARRG"
BASE COUNT          504 a         1029 c         1329 g          478 t
ORIGIN      
        1 gcggctgatc gcctccgagt tgcagaaggc ggtcaaggac ggtggcgacg acgaggtcgc
       61 cgtgctgcag cgcgagcgca agcggcggct cgagtcggcg gccgcctacc gcgacggcgg
      121 gcgcgacgac ctcgcgaccg cggaggagtc cgaggcggag ctgatcgccg catacatgcc
      181 ggagcagctc gacgacaccg agctcgcgcg gatcgtcgcc gcggcggtcg ccgagtccgg
      241 cgccagctcc ccgcgcgaga tgggcgacgt gatgaagctc gtgatgccgc aggtccgcgg
      301 ccgtgccgac ggcaagcgcg tcagcgccgc cgtgaaggag aagctgaccg cctagtgcgc
      361 cggcagatcg agctctccaa cgacgtagcc gccgagctgg ccgggcccga ggacctcatc
      421 atccgcacgc tcgagggaca cctcgactgc gaggtgttcc tgcgcggcaa cgtgctgacg
      481 ctcgatggct ccgaggacca gatcgagatg ggggcgacgg tcgtgcgcga gctgtcggac
      541 ctgattcgcc agggccacga ggtcgcccca gggacgatcg aggcgatcgg ctcggcgctc
      601 gacgagcacc agagcccgag cgagatcctc gaggacgtcg tctggcgcca ccgcggcctc
      661 aaggtcgcgc cgaagaccgt caaccagaag cgctacgtcg acgcgatccg caccaacacg
      721 atcgctgtcg ggatcggccc ggccgggacc ggcaagtcgt tcctcgcggt ggcgatggcc
      781 gtcgcggcgc tgcagcggcg cgaggtcaac cggatcgtgc tcacgcggcc cgccgtcgag
      841 gccggcgagc ggctcgggtt cctgcccggc gacctgatgg ccaaggtcga cccgtacctg
      901 cgcccgctgt tcgacgcgct ctacgacatg ctcgagccgg agaaggtcaa ccagcacctc
      961 gagcgcgggg tgatcgaggt cgcgccgctc gcgttcatgc gcggtcgcac gctcaacgac
     1021 tcgttcatca tcgtcgacga ggctcagaac accacgcccg agcagatgaa gatggtcatg
     1081 acgcgcctcg gcttcgggtc gaagatggtc gtcaccggcg acgtcaccca ggtcgacctg
     1141 ccgcgcgaga cgcactccgg cctggtcgtg atcggcgaca tcctcgagga cgtcgagggg
     1201 atcgagttcg tccgcttcgg tggcgaggac gtcgtccgcc acaagctcgt ccagcggatc
     1261 gtcgccgcct acagcgagta cgccgagtcg caggcgccgg agctgcgcaa gtcgcagtcg
     1321 cgcaggagag cctgatcgag atcgaggtca tcgacgcgcc ggcggccccg gcgggcttcg
     1381 acgcggccgc gcgcgccgcg ctcgcggcgg ccggggtcga cggtgagctg catgtcgcga
     1441 tcgccttcgt cggcgcggcg gagatccacg cgctcaaccg ccagcaccgt ggccgcgacg
     1501 agccgaccga cgtgctgtcc ttcccggtcg acggcgacgg gccggcgccc ggaccgcgcg
     1561 agctgggcga cgtcgtgatc tgccccgagc acaccgacga cctcgccgag gcggtcgtcc
     1621 acggggtgct gcacctctgt ggctacgacc acgagaccga cgacggcgag atgctggcgc
     1681 tccaggaccg cgtgatggag cggctgcggt gagcgcgacg cgcgcgggct tcgtcgcgct
     1741 cgccggacgg ccgaacgccg gcaagtcgac gctcctcaac cggatcgtcg gcgacaaggt
     1801 cgcgatcgtc tccgacaagc cgcagaccac ccggcgcgag atccgcggca tcgcgaccgg
     1861 cgacgactgg cagctcgtgc tggtcgacct gccgggcgtc cagcggccgc gcgacccgat
     1921 gaccgagcgg atgcagcggc gcgtcgagca ggcgctgcgc gacgccgacg cggtgctgtt
     1981 catgctcaac ggcgcgcagc ggatcgggcc cggcgaccgc tacatcgcgc gggcgatccg
     2041 ctcgtcgggc gtaccggcgg tgaccgccgt gaacaagatc gacaagctcg ggcgggcggc
     2101 gacggcggcc gcgctgacgg gggcggccga cctcgacgtg cagggcgaca tcttcccgat
     2161 cagcgcgaag tccggcgacg gggtggaggc gctcgtcgag catgtcgtcg ggttgctgcc
     2221 ggaggggccg tggctgtatc cgccggagga tcgctccgat caggcagagg acgtgcggat
     2281 cgcggagctg gtgcgggagc aggtgctgcg gcggacgcgg gaggagctgc cgcatgcggt
     2341 cgaggtggag gtgacgggga tcgaggagcg gggggatggg ttgctggaga tcgaggcgag
     2401 tgtgtgggcg gagagtgagt cgcagaaggg gatcttgatc gggggtcgtg gggccatggt
     2461 caaggcgatc gggaccgcgg ctcggcggga gattgagagc gttctggacc gtcgtgtgca
     2521 cttggagctg cgcgttcggg tgcggcgggg gtggcgggct gacgatgcgc tgctcgatcg
     2581 gctgggcatt gactagagct ctggccgtgc tgcccgcggg tcctgtctcc gattttggtt
     2641 cgcgtgctcg ttcctgcgcg cgcgaacgca aaatcgtcgc cacccgcgga ggccggccgc
     2701 gtggctctgg gacaatcgtc tgatggagga gattcgattc gacgacgccg ggcttgtgcc
     2761 gtgtgtgatg caggatgcgc gcacgggcga ggtgctgacg ttggcgtaca tgaacgagga
     2821 ggcgctgcgg ctgacccgtg agacggggga ggtgcacttc tggagccggt cgagggggca
     2881 gatctggcac aagggggcga cttcggggaa cacgatgaag gtgcgggcgt tgcgggtgga
     2941 ctgcgatgcg gatgcggtcg tggcgctggt cgatccgagc ggtccggcgt gccataccgg
     3001 ggcgcggagt tgcttccatt cgggggatct ggatccgcag gcgggggagg cgttggcgga
     3061 gttggagcgg acgattgcgg cgcggaaggc ggctggggat gaggggtcga gctatacggc
     3121 gcagttgttg gcggatccgc cgttgattgg cgagaaagtg cgtgaggagg cggaggaggt
     3181 gacgcgggcg gcgcgggagg agtcggatcg gcgcgtgcgg gaggaggcgg cggatgtgct
     3241 gtaccacctg gcggtgctgt tggcttcgcg ggacatgtcg tatgcggatg ctttcgagga
     3301 gctgaatgcc cgtcgcgggt agccaagggg gggggggggg
//
LOCUS       DATABV010000019        11068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_970630, whole genome shotgun sequence.
ACCESSION   DATABV010000019 DATABV010000000
VERSION     DATABV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970630"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /gene="tsaD"
                     /locus_tag="VHF88_00925"
     CDS             complement(<1..932)
                     /gene="tsaD"
                     /locus_tag="VHF88_00925"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX2160369.1"
                     /translation="MILAIETSCDDTCAAVVGDGGEIRSNKIASQGLLHERYGGVVPE
                     IASRQHLELLDAIVADALEEADVSLDDCDRIAVTRGPGLIGALLVGLSSAKALAAGRQ
                     LPLVPVDHLHGHVVASTLAPDPIEPPYVCLVASGGHTLVARVEDPSAYAILGQTLDDA
                     AGEAFDKGARILGLGYPGGPAIDRLARQGDPRAFDFPRSQPDGGEGSAGLDFSFSGLK
                     TALLYRVRDLGEAEAERRQADLAASYQAAIVDSLVSRLGQLIEREDVRHVALGGGVAA
                     NSALRSAAEGLCERLGARLWTPARELCTDNAAMIG"
     gene            complement(929..1420)
                     /gene="rimI"
                     /locus_tag="VHF88_00930"
     CDS             complement(929..1420)
                     /gene="rimI"
                     /locus_tag="VHF88_00930"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX2160370.1"
                     /translation="MTQPLEIRSLAYADLPQVIAIERRAFGTPWSLAMFVLELSKPSG
                     ICLAAVRGGRIVGYLVCSRYDTVWHVMNIAVDDRLLRQGIASALLTRLFEQADAPGEQ
                     YTLEVRPSNEPAIGLYERFGFRGAGKRRGYYHDNREDALIMWRTSGMGSGAAAARTET
                     AGG"
     gene            complement(1417..2088)
                     /gene="tsaB"
                     /locus_tag="VHF88_00935"
     CDS             complement(1417..2088)
                     /gene="tsaB"
                     /locus_tag="VHF88_00935"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX2160371.1"
                     /translation="MNVLGIDTATAASAVCVQRGDGAAFEVEPSAERLTERPAHAAEL
                     MPAVARCMDDAALGWEELDQIAVGVGPGMFTGLRIGVATARGLASATGAELRPVSSLA
                     ALADPQPSSPILALIDARRGEVFAALYDDSQPIWEPFVAAPEAVAQRLGDEGISPLAV
                     GDGAVRFRDALEAAGAAVPEAGSRSHVVRGLSICRLAAAAAPAPTGAVLPHYIRLPDA
                     KPRTA"
     gene            complement(2085..2492)
                     /gene="tsaE"
                     /locus_tag="VHF88_00940"
     CDS             complement(2085..2492)
                     /gene="tsaE"
                     /locus_tag="VHF88_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX2160372.1"
                     /translation="MILESDSAPATEAAGADLAAALRPGDVVLVTGDLGSGKTTFVRG
                     ACRALGVEVAVTSPTFTIGQVYAGRVEVAHLDLYRLDSLAGEDPALLDDYLTPDRIGF
                     VEWPAVGEMAIERVAARVRLEHAGGDRRRVEIA"
     gene            complement(2520..3053)
                     /locus_tag="VHF88_00945"
     CDS             complement(2520..3053)
                     /locus_tag="VHF88_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160373.1"
                     /translation="MSRKRKLVPSPAMAVALLALFVALGGSAYAAIVVTGKNVKNASL
                     TGVDFKKRSLHGSKLRADSIGGGSIKESTLGPVQAIAHQAAVAGSGALARGRGVTSSI
                     RVAAGRYQVAFDRDVRGCVYSATVGDVSAAGPGNGVATVASNPLNANAVDVRTFAYNN
                     AGNPVDENRSFHLLVSC"
     gene            complement(3119..4018)
                     /gene="panB"
                     /locus_tag="VHF88_00950"
     CDS             complement(3119..4018)
                     /gene="panB"
                     /locus_tag="VHF88_00950"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2160374.1"
                     /translation="MSARPKIATAPPAKGKPLTLSSLVERKALGEPIAMVTAYDYPGA
                     QVAEEAGVDVVLVGDSAAMTVLGYPSTVPVSVDEMLMLTAAVRRGLRTPILVGDLPFG
                     SYERSDEQAIETAQRFVKEAGCDAVKLERGGTSVDRARAIVEAGIPVMGHVGLTPQTA
                     TALGGYRSQGRTADRALEVARDALALQDAGCFAIVFEAIPSDLTDELMPRLEIPVIGI
                     GAGPSTDGQVLVMHDLLGIYDGHAARFVKRYAAVRDKMVAGVRAYADEVRRRQYPAPE
                     HGYTMAPDEVVRLRELLDAEVHG"
     gene            complement(4015..4395)
                     /gene="panD"
                     /locus_tag="VHF88_00955"
     CDS             complement(4015..4395)
                     /gene="panD"
                     /locus_tag="VHF88_00955"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX2160375.1"
                     /translation="MRRTMLKSKIHRATLTGSDLNYVGSITVDADLLEAADIREHEQV
                     HVLDIDNGARFETYTIAGRRGSGEMQVNGAAARLVHSGDKVIVVSYADYEEAELESYE
                     PRVVHVNGTNEIVTVDDAVAELLS"
     gene            complement(4392..5294)
                     /gene="panC"
                     /locus_tag="VHF88_00960"
     CDS             complement(4392..5294)
                     /gene="panC"
                     /locus_tag="VHF88_00960"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX2160376.1"
                     /translation="MKLVRTVAELRSALAARRDAGETVALVPTMGSFHEGHLALMRAA
                     RGACDCVVVSLFVNPTQFGAGEDLAAYPREEERDAALAAAEEVDLLFAPSVEEVYPDG
                     FATTISVGGVSEVLCGDAERRGRGHFDGVTTVVCKLFNMVGPDVAFFGQKDAQQVVVI
                     ERMVRDLDIPVRIETVATVREPDGLALSSRNAYLEPAERERATALSRALAAAERTVAS
                     GATDAAAVLAAARGELAAAGIEPEYLELRSAEDLAPAERVNGSTLLAVAARVGRARLI
                     DNTVLTSDATVSRAGAPRKGATTR"
     gene            complement(5291..6034)
                     /locus_tag="VHF88_00965"
     CDS             complement(5291..6034)
                     /locus_tag="VHF88_00965"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX2160377.1"
                     /translation="MAIVGAGRLGTALARALRAAGSSVEGPLGRDAAPQCEIVLLCVP
                     DDAIATAAARVREARFVGHVSGATPLDALGGASGEAFGLHPLQTFAGGEGPEAFRGAG
                     CAIAGRSPEALTVARELAERLGMAPIEIADAERAAYHAAASIASNFLVTLQAAAERLA
                     GGAGIAPADARALLGPLVGATVGNWIERGPEGALTGPVARGDHATVARQREAVERHAP
                     DLLDLFDELVAQTAAVAARSAPTSMVAVS"
     gene            6232..6909
                     /locus_tag="VHF88_00970"
     CDS             6232..6909
                     /locus_tag="VHF88_00970"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2160378.1"
                     /translation="MATAEQRREQLVELYREVRSCERCPLHATRTQTVFGAGNANADL
                     MFVGEAPGAQEDLQGIPFVGRAGKLLDELLAENGLARDDVFIANVLKSRPPGNRDPQP
                     EEISACQPYLFRQVELIEPTVICTLGNFSTKLLTGSNVGISRVHGVPQIHQLGRRTVQ
                     IFPIYHPAAGLRTPSVKELLREDFRKLPGLLASGPPPQEDAVHAAAAPPAEDAGEPDA
                     EQLGLFG"
     gene            6995..7687
                     /locus_tag="VHF88_00975"
     CDS             6995..7687
                     /locus_tag="VHF88_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160379.1"
                     /translation="MFRSIRTHLNPASLIAMVALFAALGGVSYAAATIDGKDIKNGSV
                     TGKKLKKGTITGSKVKADSLGGAKIKESTLGQVPSAASAQQAQSAVDAQNAVNAQNAQ
                     NATNAQTAVSAQSATSAETAATVGPNGVGTAAIQNDAVTASKLGGVVVRTGTPVSVPN
                     GASRLATASCQAGETMLSGGAIWSGISDADNVSQLHLVHSYPSAGSWGTRGYNGTGAA
                     IDLTARVVCLTN"
     gene            complement(7753..8346)
                     /locus_tag="VHF88_00980"
     CDS             complement(7753..8346)
                     /locus_tag="VHF88_00980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2160380.1"
                     /translation="MDLDATRTWWRKWRWYERNSLPWRRLAIHRELVRRGAFARWPVQ
                     GDVLELLRERRLELGEGTLLEPNVWLTAPDPARIRIGAGSFLNIGVMVAAVELVEIGD
                     HCMLANGCFVTDGAHRFDDPEKPVPWQGFTSKGPTRIGDNVWCGANVVVTSGVTIGER
                     AVIGANSVVTSDVPPRTIAAGAPAKPIRAIDYAAPSD"
     gene            complement(8383..>11068)
                     /gene="uvrA"
                     /locus_tag="VHF88_00985"
     CDS             complement(8383..>11068)
                     /gene="uvrA"
                     /locus_tag="VHF88_00985"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX2160381.1"
                     /translation="LGQMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGTVTEIYDYL
                     RLLWARIGHPHCFNCGEPIAAQSAEQIVDQVMTFDEGTRFMVEAPVIRGRKGEYGKLF
                     EELRAEGFTRVKVDGKLRRLEESIVLDKRYKHDISIVVDRLVMRHDLRKRLADSIETA
                     VAHADGMVDIEEIAEGGEGKVTTYSERFACLNCGTSMPELEPRMFSFNSPHGACPRCT
                     GLGSQMEIDPELIVPDPSLSLGEGAILPWSTSASNYYEQMTQAIAEKWEVDMDTPWQE
                     LDEQVRDTFLYGTNGDKIYVSYKNRYGRKRSYMTRFEGIVHNLERRYKETDSEYSREK
                     IEEYMSVRPCPECDGARLRPESLAVKVGGLGIHELTRMSARRTIEWFDELALTDTERQ
                     IARLILREIEERLRFLDNVGVGYLSLERAAATLSGGEAQRIRLATEIGSSLVGVLYIL
                     DEPSIGLHQRDNERLIATLERLRDLGNTVIVVEHDEGTMLAADHLVDLGPGAGEHGGE
                     LVAAGTPKQVMRVKQSLTGQFLCGARTIPLPQRRRVPAGEITIEGATQHNLRDVDVAI
                     PLGVFCCVTGVSGSGKSTLVNEVLHKAVSNRLHRTKQRPGTHRRIGGLDAVDKIINID
                     QSPIGRTPRSNPATYTGLFDSIRDLFSKTQEARARGYKPGRFSFNVKGGRCEVCRGDG
                     QIKIEMHFLPDVYVPCEQCDGRRYNRETLEVRFKGKTIADVLAMPVEEALDFFQHIPK
                     IKRRLQALHDVGLDYIRLGQPATTLSGGEAQRVKLATELAKVATGRTLYILDEPTTGL
                     HFADVERLLEMLQRLVDNGNTVVVIEHNLDVIKTADQIVDMGPEGGEEGGLVVATGTP
                     EQVAATPGSHTGRFLAEIVEPAAKQTRRGRRPRIAAAA"
BASE COUNT         1582 a         3974 c         3898 g         1614 t
ORIGIN      
        1 ccgatcatcg ccgcgttgtc ggtgcagagc tcgcgcgccg gagtccagag ccgggcgccg
       61 agccgctcgc agaggccctc cgcagccgag cgcagggcag agttggccgc gaccccgccg
      121 ccgagcgcga cgtggcggac gtcctcgcgc tcgatcagct ggccgagccg cgagacgagc
      181 gagtcgacga tcgccgcctg gtaggaggcc gccaggtcgg cctggcgccg ctcggcctcc
      241 gcctccccga ggtcgcggac gcggtagagg agcgccgtct tgagaccgga gaagctgaag
      301 tcgagaccgg ccgacccctc tcccccgtcc ggctgcgacc gtgggaagtc gaaggcccgc
      361 ggatcgcctt ggcgcgccag ccggtcgatt gccggtccgc ccgggtagcc gaggccgagg
      421 atccgcgcgc ccttgtcgaa cgcctcgccg gcggcatcgt cgagcgtctg gccgaggatc
      481 gcgtacgcgc tcggatcctc gactcgggcg acgagggtgt ggccgccgct ggcgaccagg
      541 cagacgtagg gcggctcgat cgggtccggc gcgagcgtcg aggcgaccac gtgaccgtgc
      601 aggtggtcga ccgggacgag cggcagctgc cgcccggccg cgagcgcctt cgccgacgac
      661 aggccgacga gcagcgcacc gatcaagccg ggcccgcgcg tcaccgcgat gcgatcgcag
      721 tcgtcgagcg acacgtccgc ctcctcgagc gcgtcggcga cgatcgcgtc gagcagctcg
      781 aggtgctggc gcgaggcgat ctccggcacg acgccgccgt agcgctcgtg cagcagcccc
      841 tgcgaggcga tcttgttcga gcggatctcg cccccgtcgc cgacaaccgc cgcgcaggtg
      901 tcgtcgcagc tcgtctcgat cgcgaggatc atccgcccgc cgtctcggtc cgcgcggccg
      961 cggcgcccga gcccatcccg ctggtccgcc acatgatcag tgcgtcctcg cggttgtcgt
     1021 ggtagtagcc gcgccgcttg ccggctcccc ggaagccgaa tcgctcgtag agcccgatcg
     1081 ccggctcgtt cgaaggtcgc acctcgagcg tgtactgctc gccgggagcg tcggcctgct
     1141 cgaagaggcg cgtcagcagg gcgctggcga tcccctgacg cagcagccgg tcgtcgacgg
     1201 cgatgttcat cacgtgccag acggtgtcgt agcgcgagca gaccaggtag ccgacgatcc
     1261 gcccgccgcg gaccgcggcg aggcagatcc ccgacggctt cgacagctcg agcacgaaca
     1321 tcgcgagcga ccacggcgtg ccgaacgcgc gccgctcgat cgcgatcacc tggggcagat
     1381 cggcgtaggc gaggctcctg atctcgagcg gctgggtcac gcggtcctcg gcttcgcgtc
     1441 cggaaggcgg atgtagtggg gaagcacagc cccggtcgga gccggcgccg cggctgccgc
     1501 cagccggcag atcgagaggc cgcgcaccac gtgggagcgc gagccggctt ccgggaccgc
     1561 tgcgcccgcc gcctcgaggg cgtctcgaaa gcgtaccgcg ccgtcgccta ctgccaaagg
     1621 gctgattccc tcatccccga gccgctgcgc caccgcctcc ggtgcggcga cgaagggctc
     1681 ccagatgggc tgcgagtcgt cgtacagcgc ggcgaagacc tcgccgcggc gggcgtcgat
     1741 cagggccaga atcggagagc tgggctgagg gtccgcgagc gccgcgagcg aggacaccgg
     1801 ccgcagctct gcgcccgtcg ccgaggcgag gccacgcgcg gtcgcgaccc cgatccgcag
     1861 gccggtgaac atgcccggcc cgacgccgac ggcgatctgg tcgagctcct cccatccaag
     1921 cgcggcgtcg tccatgcagc gcgcaaccgc cggcatcagc tcggcggcgt gggcgggtcg
     1981 ctcggtcagc cgctccgcgc tcggctcgac ctcgaacgcg gccccgtcgc cgcgctgcac
     2041 gcagacggca ctggcggccg tcgccgtgtc gatccccagg acgttcacgc gatctccacg
     2101 cgccgccggt cgccgccggc gtgctccagg cgcacgcgcg ccgccacacg ctcgatcgcc
     2161 atctccccga ccgccggcca ctcgacgaag ccgatccggt caggcgtcag gtagtcgtcg
     2221 agcagcgccg gatcctcgcc cgcgagcgag tcgagccgat agagatccag gtgggcgacc
     2281 tcgacgcggc cggcgtagac ctgaccgatc gtgaacgtcg ggctggtcac ggcgacctcg
     2341 acccccagcg cccggcaggc gccgcgtacg aacgtcgtct tgccgctgcc caggtcgccc
     2401 gtgacgagga cgacgtcccc gggtcgcaga gccgctgcga gatcggcgcc ggcggcctcg
     2461 gtcgccggcg cgctatcgct ctccaggatc acacggcgaa gtctgaacca cccatcccgt
     2521 cagcagctga ccagcaggtg gaacgaccgg ttctcgtcga ccgggttgcc cgcgttgttg
     2581 taggcgaagg tgcgcacgtc gacggcgttg gcgttgagtg gattgctcgc taccgtcgcc
     2641 acgccattgc ccggcccggc cgcgctgacg tcgccaacgg tcgcgctgta gacgcagccg
     2701 cgcacgtcgc ggtcgaaggc cacctggtag cgcccggcgg cgacgcggat cgagctcgtc
     2761 acgccacgcc cgcgtgcgag tgcgccgctg ccggccacgg cggcctggtg ggcgatcgcc
     2821 tggacgggac cgagcgtcga ctccttgatc gatccgccgc cgatcgagtc ggcacgcagc
     2881 ttcgagccgt gcagcgaccg cttcttgaag tcgacgccgg tgaggctcgc gttcttgacg
     2941 ttcttgccgg tcacgacgat cgcggcgtag gcgctgccgc cgagcgcgac gaacagggcc
     3001 aggagggcca ccgccatcgc tggtgagggc acgagcttgc gcttgcggga catggaggct
     3061 gcctcctagg tcgagtgggg tctctccccc tttcaacccg gccaatcccc cgaagtagct
     3121 agccgtgaac ctccgcgtcg agcaactcgc gcagccgaac gacctcatcg ggggccatcg
     3181 tgtacccgtg ctcgggcgcc gggtactggc gccggcgcac ctcgtcggcg tacgcgcgga
     3241 cgcccgcgac catcttgtcg cggaccgccg catagcgctt gacgaagcgt gcggcatgtc
     3301 cgtcgtagat gccgagcagg tcgtgcatca ccaacacctg accgtcggtc gacgggcccg
     3361 cgccgatgcc gatgaccggg atctcgagcc gcggcatcaa ctcgtcggtc aggtccgacg
     3421 gaatcgcctc gaagacgatc gcgaagcagc ccgcgtcctg gagcgcgagc gcgtcacgcg
     3481 ccacctcgag cgcccggtcc gcggttcgcc cctgcgagcg gtagccgccg agcgcggtcg
     3541 cggtctgcgg cgtcaggccg acgtggccca tcaccggtat gccggcctcg acgatcgccc
     3601 gtgcccggtc gacggacgtg ccgccgcgct cgagcttgac ggcgtcgcag ccggcctcct
     3661 tgacgaagcg ctgcgccgtc tcgatcgcct gctcgtcgga gcgctcgtag gagccaaacg
     3721 ggagatcgcc gacgaggatc ggggttcgca ggccacggcg gacggcagcc gtcagcatca
     3781 gcatctcgtc gaccgagacc gggacggtgc tcggatagcc aagcaccgtc atggccgcgg
     3841 agtccccgac cagcacgacg tcgacgccag cctcctcggc gacctgggcg cctgggtagt
     3901 cgtaggcggt gaccatcgcg atcggctcgc cgagcgcctt gcgctcgacc agcgacgaga
     3961 gcgtgagggg cttgcccttg gcgggtggag cggtcgcgat cttcggccgg gcgctcacga
     4021 cagcagctcc gcgaccgcat cgtcgaccgt gacgatctcg tttgtgccgt tgacgtggac
     4081 tacgcgcggc tcgtaggact ccagctccgc ctcctcgtag tcggcgtagg agacgacgat
     4141 caccttgtcg ccgctgtgaa ccagccgggc ggcggcgccg ttgacctgca tctcgcccga
     4201 gccgcggcgg ccggcgatcg tgtaggtctc aaagcgggcg ccgttgtcta tgtcgagcac
     4261 gtgcacctgc tcgtgctcgc ggatgtcggc ggcctcgagc aggtcggcgt cgacggtgat
     4321 cgagccgacg tagttgaggt cgctccccgt cagggtggcg cggtggatct tggacttgag
     4381 catcgtccgt ctcatctggt ggtggctcct tttcgggggg cgccggcgcg cgagacagtc
     4441 gcatccgacg tgaggacggt gttgtcgatc aggcgggcgc gcccgacacg ggcggccacg
     4501 gcaagcaggg tcgagccgtt gacgcgctcg gcaggcgcca ggtcctcggc cgagcgcagc
     4561 tcgaggtact ccggctcgat cccggcggcg gcgagctcgc cgcgtgcagc ggcgaggacg
     4621 gcggcggcgt cggtggcgcc cgaggccacg gtccgctcgg cggccgccag cgcgcgactg
     4681 agcgctgtcg cgcgctcgcg ctccgccggc tcgaggtagg cgttgcgaga gctgagcgcg
     4741 aggccgtcgg gctcgcgcac cgtagccacg gtctcgatcc gcacggggat gtcgaggtcg
     4801 cgcaccatcc gctcgatcac gacgacctgc tgggcgtcct tctggccgaa gaaggcgacg
     4861 tctggcccga ccatgttgaa gagcttgcag acgacggtgg tgaccccgtc gaagtggccg
     4921 cgtccgcgtc gctcggcgtc gccgcagagg acctcgctca cgccgccgac gctgatcgtg
     4981 gtggcaaagc cgtcggggta gacctcctcg accgagggcg cgaagagcag gtcgacttcc
     5041 tcagcggcgg cgagcgcggc atcgcgctcc tcctcgcgcg ggtaggcggc gaggtcctcg
     5101 cccgcgccga actgggtcgg gttgacgaac agcgagacga cgacgcagtc gcaggctccg
     5161 cgcgcggcgc gcatcagcgc gaggtggccc tcgtggaagg agcccatcgt cgggaccagc
     5221 gcgaccgtct cgccggcgtc acgccgcgcg gcgagcgcgc tgcgcagctc ggcgaccgtg
     5281 cggaccagct tcacgagacc gccaccatcg aggtcggggc ggacctcgcc gcgacggcgg
     5341 cggtctgcgc gaccagctcg tcgaacagat cgagcaggtc cggcgcgtga cgctcgacgg
     5401 cctcgcgttg gcgcgcgacc gtcgcgtggt cgccccgcgc gaccgggccc gtcagcgcgc
     5461 cctcggggcc gcgctcgatc cagttgccga ccgtcgcgcc caccaacggg ccgagcaggg
     5521 cgcgcgcgtc ggctggcgcg atacccgctc cgccggccag ccgctcggcg gccgcctgga
     5581 gggtgacgag gaagttcgag gcgatcgatg ccgccgcgtg gtaggcggcc cgctcggcgt
     5641 cggcgatctc gatcggcgcc atgccgagcc gctcggcaag ctcgcgtgcg accgtcagcg
     5701 cctccgggct cctcccggcg atcgcacagc cggcgccgcg aaacgcctcc ggaccctcgc
     5761 cgccggcgaa ggtctgcagc ggatgcaggc cgaacgcctc tccgctcgcg ccgccgagcg
     5821 cgtcgagcgg cgtcgcaccg ctgacatgac ctacgaatcg ggcctcgcgg acgcgcgcag
     5881 cggcggtcgc gatcgcgtcg tccggtacgc agagcaggac gatctcgcac tgaggcgcgg
     5941 cgtcgcggcc gagcgggccc tcgacggagc tgccggcagc gcgcagcgct cgagccagcg
     6001 ccgttccgag ccggccggcg ccgacgatcg ccacgctcgc atgggcgtgg ccgggggttg
     6061 tggtggcctg tgggatctcc gttccagtcc ctgggggtac cggtcggtct ggctgacggt
     6121 ccactcccga gctgggtagc ggccgtgcgg tcaaggatag cgccgcttgc ggcagtcggc
     6181 caattccgtc gcggcttcga gccgccgctc gccgaactag gctgacccgc gatggcgact
     6241 gccgagcaac ggcgcgagca gctggtcgag ctctaccgcg aggtgcgcag ctgcgagcgc
     6301 tgtccgctcc acgccacgcg cacccagacc gtcttcggcg ccggcaacgc caacgccgac
     6361 ctgatgttcg tcggcgaggc gcccggcgca caggaggatc tgcaggggat cccgttcgtc
     6421 ggacgtgcgg gcaagctgct cgacgagctg ctggccgaga acggcctcgc gcgcgacgac
     6481 gtattcatcg cgaacgtcct gaagagccgc ccgcccggca accgcgatcc ccagccggag
     6541 gagatatcgg cctgccagcc ctacctcttc cgccaggtcg agctgatcga gccgacggtc
     6601 atctgcacgc tcggcaactt ctcgacgaag ctgctcaccg gctccaacgt cggcatcagc
     6661 cgcgtccacg gtgtcccgca gatccaccag ctcggacggc gaacggtgca gatcttcccg
     6721 atctaccacc cggccgcggg cctgcgtacg ccgagcgtca aggagctgct gcgcgaggac
     6781 tttcgcaagc tgcccgggct actcgcgagc ggtccgccgc cgcaggagga cgccgtgcat
     6841 gccgccgccg ctccacccgc cgaggacgcc ggcgagcccg acgccgagca gctcggtctc
     6901 ttcggctgag aatttttttc gatcgcggcg taagcaacgg ccggctcccc ggttggacgg
     6961 gtgtcaatca caccgtccga ccaggaggtc gaccatgttc cgcagcatcc gcacacatct
     7021 caacccggcc tcgctgatcg cgatggtcgc cctgttcgcc gcgctcggcg gcgtctcgta
     7081 cgccgccgcg acgatcgacg gcaaggacat caagaacggc agcgtgaccg gcaagaagct
     7141 gaagaagggc acgatcaccg gcagcaaggt caaggccgac tcgctcggcg gcgccaagat
     7201 caaggagtca acgctcggcc aggtcccgag tgccgcgagc gcccagcagg cacagagcgc
     7261 cgtcgacgcc cagaacgccg tcaacgctca gaatgcccag aacgcgacca acgcacagac
     7321 cgcggtcagc gcgcagagcg ccacgagtgc ggagacggct gcgacggtcg gaccgaacgg
     7381 cgtcggcacg gcggcgatcc agaacgacgc ggtcaccgcg tcgaagctcg gcggcgtcgt
     7441 cgtgcgcacc gggacgccgg tctcagtccc gaacggagcc tcacgactgg cgacggccag
     7501 ctgccaggcc ggcgagacga tgctctccgg cggtgcgatc tggagcggca tcagcgatgc
     7561 cgacaacgtg tcgcagctcc acctcgtcca ttcgtacccg tcggccggaa gctggggcac
     7621 gcggggctac aacggcaccg gcgcggcgat cgacctgact gcacgcgtgg tctgcctcac
     7681 gaactgaatc gcaacctccc gtaggcgcgt cgacgggccg gggccttgct ccggcccgtc
     7741 tgcgttgccg gctcagtcgc tcggcgccgc gtagtcgatt gcgcggatcg gcttagccgg
     7801 cgctccagcg gcgatcgtcc gtggcggcac gtcgctggtc acgacgctgt tcgcgccgat
     7861 caccgcgcgc tcgccgatcg tcaccccgct cgtcacgacg acgttcgccc cgcaccagac
     7921 gttgtcgccg atccgcgtcg gccccttgct cgtgaagccc tgccacggca ccggcttctc
     7981 aggatcatcg aagcggtgcg cgccgtcggt cacgaagcag ccgttggcga gcatgcagtg
     8041 gtcgccgatc tcgaccagct cgacggcggc gaccatcacg ccgatgttca ggaagctgcc
     8101 ggcgccgatc ctgatccgcg ccggatccgg cgccgtcagc cagacgttcg gctcgagcag
     8161 cgtgccctcc cccagctcga gccgtcgctc gcgcagcagc tcgagcacgt cgccctggac
     8221 cggccagcgc gcgaaggccc cgcgccgcac gagctcacga tggatcgcga gccgtcgcca
     8281 cggcagggag ttgcgctcat accagcgcca cttccgccac caggtgcgcg tcgcgtcgag
     8341 gtccaccggc gcagcttccc accagcgacg cctggctggc ggtcaggccg ccgccgcgat
     8401 gcgcggcctg cgaccgcgtc gcgtctgctt cgcggccggc tcgacgatct ccgcgaggaa
     8461 gcgaccggtg tgcgatccgg gcgtcgcggc gacctgctcg ggtgtgccgg tggcgacgac
     8521 gaggccgccc tcctcgcccc cttcgggacc catgtcgacg atctggtcgg cggtcttgat
     8581 gacgtcgagg ttgtgctcga tgacgacgac cgtgttgccg ttgtcgacca gccgctggag
     8641 catctcgagc agccgctcga cgtcggcgaa gtgcagaccg gtcgtcggct cgtcgaggat
     8701 gtagagcgtc cgcccggtcg cgaccttcgc cagctctgtc gccagcttga cgcgctgcgc
     8761 ctcgccgccg gagagggtcg tcgccggctg gccgaggcgg atgtagtcga gcccgacgtc
     8821 gtgcagggcc tgcagccggc gcttgatctt cgggatgtgc tggaagaagt cgagcgcctc
     8881 ctcgaccggc atcgccagca cgtcggcgat cgtcttgccc ttgaagcgga cctcgagcgt
     8941 ctcgcggttg tagcgccgtc cgtcgcactg ctcgcacggc acgtagacgt cgggcaggaa
     9001 gtgcatctcg atcttgatct ggccgtcgcc gcggcagacc tcgcagcgac cgcccttgac
     9061 gttgaacgag aagcggcccg gcttgtagcc gcgggcgcgc gcctcctggg tcttcgagaa
     9121 gaggtcgcgg atcgagtcga acagcccggt gtaggtcgcc gggttggagc gcggcgtgcg
     9181 tccgatcggc gactggtcga tgttgatgat cttgtcgacg gcgtcgagac cgccgatccg
     9241 gcggtgggtg ccggggcgct gcttggtgcg gtgcagccgg tttgagaccg ccttgtgcag
     9301 cacctcgttg accagcgtcg acttgcccga ccccgacacg cccgtcacgc agcagaagac
     9361 gccgagcggg atcgcgacat cgacgtcgcg caggttgtgc tgcgtcgccc cctcgatcgt
     9421 gatctcgccg gccgggacgc gacgacgctg cggcagcgga atcgtccgcg cgccgcagag
     9481 gaactggccg gtcagcgact gcttgactcg catcacctgc ttcggcgtgc cggccgcgac
     9541 cagctcgccg ccgtgctcgc cggcaccggg cccgaggtcg acgaggtgat cggcggcgag
     9601 catcgtgccc tcgtcgtgct cgacgacgat caccgtgttg ccgaggtcgc gcaaccgctc
     9661 gagcgtcgcg atcagccgct cgttgtcgcg ctgatgcagg ccgatcgacg gctcgtcgag
     9721 gatgtagagc acgccgacga ggctcgagcc gatctccgtc gcgaggcgga tccgctgggc
     9781 ctcgccgccg gacaacgtcg cggcggcgcg ctcgagcgac aggtagccga ccccgacatt
     9841 gtcgaggaag cgcagccgct cctcgatctc gcgcaggatc agccgcgcga tctggcgctc
     9901 ggtgtcggtc agcgcgagct cgtcgaacca ctcgatcgtc cgccgcgccg acatccgggt
     9961 cagctcgtgg atgccgaggc cgccgacctt gaccgccagg ctctccgggc gcagccgcgc
    10021 gccgtcgcac tccgggcacg ggcggaccga catgtactcc tcgatcttct cgcgggagta
    10081 ctcggagtcg gtctccttgt agcggcgctc gaggttgtgg acgatcccct cgaagcgggt
    10141 catgtacgac cgcttgcgtc cgtaacggtt cttgtaggag acgtagatct tgtcgccatt
    10201 ggtgccgtag aggaaggtgt cgcgcacctg ctcgtcgagc tcctgccagg gagtgtccat
    10261 gtcgacctcc cacttctcgg cgatcgcctg ggtcatctgc tcgtagtagt tcgacgcgct
    10321 cgtcgaccac ggcaggatcg ccccctcgcc gagcgacagc gaggggtccg ggacgatcag
    10381 ctcggggtcg atctccatct gcgatccgag cccggtgcag cgcgggcagg cgccgtgggg
    10441 cgagttgaac gagaacatcc gcggctcgag ctccggcatc gaggtgccgc agttgaggca
    10501 ggcgaagcgc tcggagtagg tcgtgacctt gccctctccc ccttccgcga tctcctcgat
    10561 gtcgaccatc ccgtcggcgt gggcgaccgc cgtctcgatc gagtcggcga gccgcttgcg
    10621 caggtcgtgg cgcatcacga gccggtccac gacgatcgag atgtcgtgct tgtagcgctt
    10681 gtcgagcacg atcgactcct cgagccggcg caacttgcca tcgaccttta cgcgcgtgaa
    10741 cccctccgcg cgcagctcct cgaacagctt gccgtactcg cccttgcgcc cgcggatcac
    10801 cggcgcctcg accatgaagc gggtgccctc gtcgaaggtc atcacctggt cgacgatctg
    10861 ctcggccgac tgcgcggcga tcggctcgcc gcagttgaag cagtgcgggt ggccgatccg
    10921 cgcccagagc aggcgcaggt agtcgtagat ctcggtgacc gtgccgaccg tcgagcgcgg
    10981 gttgcgcgag gtcgtcttct ggtcgatcga gatcgccggc gagaggccct cgatcgagtc
    11041 gacgtcgggc ttgtccatct ggccgagg
//
LOCUS       DATABV010000020         6659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1613386, whole genome shotgun sequence.
ACCESSION   DATABV010000020 DATABV010000000
VERSION     DATABV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613386"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..861)
                     /gene="rplJ"
                     /locus_tag="VHF88_00990"
     CDS             complement(88..861)
                     /gene="rplJ"
                     /locus_tag="VHF88_00990"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX2160382.1"
                     /translation="MNRDEKAAVIDQVAEQIQESQAVFALDYRGITVAQAAELRARLR
                     EADASLRVVKNTLTERAADKAGAEDLKGFLDGPTAFTFVRGDAALAAKALATFRREHE
                     LLEFKGGTMDGGPVSADEIVAISKLPSRDALNQQLVGVLAYPIGGLARTLNQLIQGLA
                     VQLGQIAEQGLVGGDAPEPEPEPEPEPEPAAETEAAAESEAAAETEPAAEPEAKADEP
                     AEADTTEEAEAPAAAAESAEQSADTDQDDTPAEGEDKES"
     gene            complement(1001..1726)
                     /gene="rplA"
                     /locus_tag="VHF88_00995"
     CDS             complement(1001..1726)
                     /gene="rplA"
                     /locus_tag="VHF88_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX2160383.1"
                     /translation="MAKHGKRYVDNLAKVDREREYTPTEAIALIKSFDSGKLDETVEV
                     HIRTGLNVRHAEEQLRGTISLPHGLGREMTVAVFAKGDKAREAQEAGADHVGDDDLAK
                     KVEDGFSDFDVAIATPDMMPVVGRLGRVLGPQGKMPNPKVGTVTNDVTKAVSEAKAGK
                     IEYRTDRTAIVHLPIGKASFEASALVENFAALIDEVNRAKPASAKGRYLETVTVTTTM
                     GPGVRVDPSATADNAGEAAAAVA"
     gene            complement(1729..2151)
                     /gene="rplK"
                     /locus_tag="VHF88_01000"
     CDS             complement(1729..2151)
                     /gene="rplK"
                     /locus_tag="VHF88_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX2160384.1"
                     /translation="MAKKVLTLIKLQVPGGNANPAPPVGPALGQHGVNIMEFCKAFNA
                     QTQGDNGTIIPVEITVFEDRSFTFITKTPPAAILIKQAAGISKGSGEPNRDKVASITR
                     DKARQIAEKKLPDLNANDLDQATKIIEGTARSMGVEVS"
     gene            complement(2153..2674)
                     /gene="nusG"
                     /locus_tag="VHF88_01005"
     CDS             complement(2153..2674)
                     /gene="nusG"
                     /locus_tag="VHF88_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX2160385.1"
                     /translation="MFRWYAVNTYSGHEKKVKQNLEHRVQSLGQQVAVRQVVIPTEQV
                     QEMKDGKKVEKEQRTMPGYLLINMNLTDDSWGLVKNTPGVTGFVGPSQKPVPLSQPEV
                     DRLLNREIAERPRTRAQFTVGESVKVVSGPLSDFSGEISEINDDAAKLKVLVSIFGRE
                     TPVEVGFDQVKKI"
     gene            complement(2718..3314)
                     /gene="secE"
                     /locus_tag="VHF88_01010"
     CDS             complement(2718..3314)
                     /gene="secE"
                     /locus_tag="VHF88_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX2160386.1"
                     /translation="MARNRQRAKERQARRKAKGGGDGAAKQPRASDPNAAKPGPTRRQ
                     GDDPIADDVLDELERESVVAAGADPATPPPLSESERLRAGAPPEDVGSSAEVLDFEQD
                     LEADDDPHHFDPAQDVPEEDLSDDGKQRRKPVGEHKDRPRFVQFLVAVWAELQRVQWP
                     DRQAMTTLTGVVLGFVLIAGGYLGLLDAIASRVIQAIL"
     gene            complement(3354..3518)
                     /gene="rpmG"
                     /locus_tag="VHF88_01015"
     CDS             complement(3354..3518)
                     /gene="rpmG"
                     /locus_tag="VHF88_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX2160387.1"
                     /translation="MARGDVRIAVTLECQDCKRRNYQSQKSKRNNPDRIELRKYCRWC
                     GKHTPHKETR"
     gene            3576..4481
                     /locus_tag="VHF88_01020"
     CDS             3576..4481
                     /locus_tag="VHF88_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2160388.1"
                     /translation="MELDVRTLHGKRVAFRRAGSGPPLFLIHGITNSGQSWEPAMRLL
                     ARDFDVIAPDLPGHGNSDRQRGDHSLGIHACVMRDLLHVLEIDRVTVVGHSLGGGVAM
                     QFSYQFPEMVERLVLVGSGGLGREVSPLVRSAALPFAEQVLPLLTARPLVDSVTAIAG
                     LLGKVGLKPGADLAEISRGIASLGDTERRAAFVRTVRSVMSPRGQRVTANDRLYLAAE
                     TPMLIVWGERDPIIPVEHGLAAHAQLPNSRLEVFENAGHFPQLDDPLRFAELLTDFVA
                     DTEPAVHDADTVRARLVEHAAAASA"
     gene            4516..5823
                     /locus_tag="VHF88_01025"
     CDS             4516..5823
                     /locus_tag="VHF88_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX2160389.1"
                     /translation="MSQTPPEWRNWTGDQVCRPAAIERPASTAEVAAAIERARDAGRT
                     LRVAGAGHSFTDAALTDGTLLRLDRMRRVLDVDRASGLARVEAGISLRELSETLWAHG
                     LAFENLGDIDVQSIAGATATGTHGTGARLRNLSAALREVELVTADGTTARVSEDADPD
                     AWRAARIGVGALGVVTAVTLQAVPAFVLEGVDSTAPLEELLDGLDERADGADHFELYA
                     FPHSPVAVTRTNRRADRPPAPPSRLSAWAGDVLLRNYVLGAAFELGRRAPRSIPRMHR
                     ALTSIAGTTRRVDRSYRVFASPRLVRFTEMEYAVPRARAADAVRAVLAVARRADLAVP
                     MPIEVRFVAPDDAFLSPAGGRETCYVAVHMYRGMEWASYFRAIEEVMDGFEGRPHWGK
                     RHFQTAATLGPRYSEWERFQAVRRRLDPEGLFANGYVRRVLGA"
     gene            5890..6330
                     /locus_tag="VHF88_01030"
     CDS             5890..6330
                     /locus_tag="VHF88_01030"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2160390.1"
                     /translation="MAGSSSAEIDAPLEQVWKAVEDVEKAPDWQGGMKDIEALERDGE
                     GRAVLVETEADAKVTTIKTKVRFSYDGPTRLSWKQEKGDLKSMVGSWDLEDLGDGRTR
                     ATYMLEGDTGRMLGMLIRGPVEDKLRDFLVNARADELKAYVEGS"
     gene            complement(6327..>6659)
                     /locus_tag="VHF88_01035"
     CDS             complement(6327..>6659)
                     /locus_tag="VHF88_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160391.1"
                     /translation="GTGGPAAPIAALTLAEARSAMRRGMRGRVGRRAKIGVRRCRQLS
                     VVRVRCRVTARRGALAWKGSATVTERAAATGVSRTFAFRGKRTDAACLRHARASQSTG
                     ACVRRVRL"
BASE COUNT          851 a         2323 c         2390 g         1095 t
ORIGIN      
        1 cgagcgcctt gatccgctcg gacagctcga gcaccgagat gctcttgagc tcgtcgatcc
       61 actcctcggt agttgctgca gccatctcta gctctccttg tcctcgccct ctgcgggcgt
      121 gtcgtcttgg tcggtgtccg ccgactgctc ggcggactcg gccgcggcgg ctggcgcctc
      181 ggcctcctca gtcgtgtcgg cctcggcggg ctcgtccgcc ttagcctcgg gctccgccgc
      241 tggctcggtc tcggcggcgg cctcggactc cgccgcggcc tcggtctcgg cggcgggctc
      301 cggctccggc tccggctccg gctccggctc cggagcgtcg ccgcccacga ggccctgctc
      361 ggcgatctgg ccgagttgga cggcgaggcc ctggatcagc tggttgagcg tgcgcgccag
      421 gccgccgatc gggtacgcga ggaccccgac gagctgctgg ttgagcgcgt cgcgcgacgg
      481 cagcttcgag atcgcaacga tctcgtcggc cgataccggg ccgccgtcca tcgtgccgcc
      541 cttgaactcg agcagctcgt gctcgcgacg gaacgtcgcc agcgccttgg cggcgagcgc
      601 ggcgtcgccg cgaacgaagg tgaaggccgt cgggccgtcg aggaagccct tcaggtcctc
      661 ggcgccggcc ttgtcggccg cacgctcggt gagcgtgttc ttgacgacgc gcaggctggc
      721 gtcggcctcg cgcaggcgtg cccgcagctc ggccgcctgg gcgaccgtga tcccgcggta
      781 gtcgagcgcg aagaccgcct gcgactcctg gatctgctcg gccacctgat cgatgaccgc
      841 tgccttctcg tccctgttca taccgttgtc tccaatgaaa gagcccgctc acaggcgggc
      901 tcgggaggac ggttcgggac cggtctcgcg cgctccacct gccccggact tccggccgct
      961 cctggcggcc cgccgagggt ctcgggcaga aactcgttgg tcaggctaca gcagccgcag
     1021 cctctccagc gttgtcggcg gtcgccgacg ggtcgacgcg cacgccgggg cccatcgtcg
     1081 tggtgaccgt cacggtctca aggtagcggc ccttcgccga cgccggcttg gcgcggttga
     1141 cctcgtcgat caacgcggcg aagttctcga ccagcgcgct cgcctcgaag ctcgccttgc
     1201 cgatcggcag gtggacgatc gcggtgcggt cggtgcggta ctcgatcttg cccgccttgg
     1261 cctcggacac cgccttggtg acgtcgttgg tgacggtgcc gaccttcggg ttgggcatct
     1321 tgccctgcgg tccgagcacg cggccgagcc tgccgacgac cggcatcatg tcgggcgtcg
     1381 cgatcgcgac gtcgaagtcg ctgaagccgt cctccacctt cttggctagg tcgtcgtcgc
     1441 cgacgtggtc ggcgccggcc tcctgggcct cgcgtgcctt gtcgcccttg gcgaagacgg
     1501 cgacggtcat ctcacggccg agcccgtgcg gcagcgagat cgtcccgcgc agctgctcct
     1561 cggcgtggcg gacgttgagc ccggtgcgga tgtggacctc gaccgtctcg tcgagcttgc
     1621 cgctgtcgaa gctcttgatc agcgcgatcg cctcggtcgg cgtgtactcg cgctcgcggt
     1681 cgaccttggc gaggttgtcg acgtagcgct tgccgtgctt ggccatcgct acgagacctc
     1741 cacgcccatc gagcgcgcag tgccctcgat gatcttggtc gcctggtcga gatcgttcgc
     1801 gttgaggtcc ggcagcttct tctcggcgat ctggcgcgcc ttgtcgcgcg tgatcgaggc
     1861 gaccttgtcg cggttcggct ccccggagcc ctttgagatc ccggctgcct gcttgatcag
     1921 gatcgccgcc ggcggggtct tggtgatgaa ggtgaacgag cggtcctcga agaccgtgat
     1981 ctcgacgggg atgattgtcc cgttgtcgcc ctgggtctgc gcgttgaacg ccttgcagaa
     2041 ctccatgatg ttgacgccgt gctggcccag cgcgggaccg accggcggcg ccgggttggc
     2101 gttgccgccg ggcacctgca gcttgatgag tgtcaggact ttcttggcca tctcagatct
     2161 tcttcacctg gtcgaagccg acctcgaccg gcgtctccct accgaagatg gataccagca
     2221 ccttgagctt ggcggcgtcg tcgttgatct cggagatctc gccggagaag tccgagagcg
     2281 gcccggagac caccttgacc gactcgccga cggtgaactg ggcgcgcgtc cgcggccgct
     2341 cggcgatctc gcggttgagc agccggtcga cctctggctg gctcagcggg accggcttct
     2401 gcgacggccc gacgaagccg gtaacgcccg gggtgttctt gaccaggccc cacgagtcgt
     2461 cggtcaggtt catgttgatg agcaggtagc cgggcatcgt ccgctgctcc ttctcgacct
     2521 tcttgccgtc cttcatctcc tggacctgct cggtcgggat gacgacctgg cgcacggcca
     2581 cctgctggcc gagcgactgc acccggtgct cgaggttctg cttgaccttc ttctcgtggc
     2641 ccgagtaggt gttgacggcg taccagcgga acatgacttg actcagattg ctttctctgg
     2701 aggtggcccg cgaggggcta gaggatggct tggatgactc gtgatgcgat cgcgtcgagc
     2761 agaccgaggt agccgccggc gatcaacacg aagcccagga cgacccccgt cagcgttgtc
     2821 atcgcctgac ggtcaggcca ctgcacacgt tgcagctccg cccatacggc gacgaggaac
     2881 tgtacgaaac gcgggcggtc cttgtgctct cccaccggtt tgcgacgttg cttgccgtcg
     2941 tcggagaggt cctcctcggg aacgtcctgg gcgggatcga agtggtgtgg gtcgtcgtcg
     3001 gcctcgagat cctgctcgaa gtcgagcacc tcggcggagc tgccgacgtc ctccggcggg
     3061 gcgccggcgc gcaggcgctc ggactcgctc aacggcggcg gcgtcgcggg gtcggcgccc
     3121 gcggcgacga ccgactcgcg ctcgagctcg tcgaggacgt cgtcggcgat cggatcgtcg
     3181 ccctggcgac gggtcggtcc cggcttggcg gcgttcggat cggacgcgcg cggctgcttg
     3241 gcggcgccgt cgcctccccc cttcgccttg cggcgcgcct ggcgctcctt ggcccgctga
     3301 cggttccgag ccacgtggct ccctctactt cacgcgcgtg agcgcaaggg cgctcagcgc
     3361 gtctccttgt gcggcgtgtg cttgccgcac cagcggcagt acttgcgcag ctcgatccga
     3421 tccggattgt tgcgcttgga cttctgtgac tggtagttgc gccgtttgca gtcctggcac
     3481 tccagggtca cggcgatcct gacgtctcca cgggccatag ccacccaaga tagcgtggct
     3541 cgggttcagc cgccgggtgg gtacagtcgg gggcgatgga gctcgacgtc aggacactgc
     3601 atggcaagcg ggtcgccttc cggcgcgccg gctcgggccc gccgctcttc ctgatccacg
     3661 ggatcacgaa cagcgggcag agctgggagc ccgcgatgcg cctgctggcg cgcgacttcg
     3721 acgtgatcgc gcccgacctt ccgggccacg gcaactccga ccgccagcgc ggtgaccatt
     3781 cgctcggcat ccacgcgtgc gtgatgcgcg acctgctgca tgtgctcgag atcgaccgcg
     3841 tgacggtcgt cgggcactcg ctcggcggcg gggtcgcgat gcagttctcc taccagttcc
     3901 ccgagatggt cgaacggctc gtgctggtag gcagcggcgg gctcgggcgc gaggtcagcc
     3961 cgctggtgcg cagcgcggcg ctgccgttcg ccgagcaggt gctgccgctg ctgaccgcgc
     4021 ggccgctggt cgacagcgtc acggcgatcg ccggcctgct cggcaaggtc ggtctcaagc
     4081 cgggcgccga cctggcggag atctcacgcg ggatcgcctc gctcggggac accgagcgtc
     4141 gggcggcatt cgtgcggacg gtgcggtcgg tcatgagccc gcgcggccag cgcgtgacgg
     4201 ccaacgaccg gctctacctc gccgccgaga cgccgatgct gatcgtctgg ggcgagcgcg
     4261 acccgatcat cccggtcgag cacgggctcg ccgcccacgc gcagctgccg aacagccggc
     4321 tcgaggtctt cgagaacgcg ggccacttcc cgcagctcga cgacccgctc cgcttcgcgg
     4381 agctgctgac cgacttcgtc gcagacaccg agcccgcggt gcacgacgcc gataccgtgc
     4441 gcgcgcgcct cgtcgagcac gccgccgccg caagcgcctg agccactcgg ccggcgagat
     4501 agcatcgccg cgccgatgtc gcagacccca cccgagtggc gcaactggac cggcgaccag
     4561 gtctgccgtc cggccgcgat cgagcgaccc gcgagcacgg cggaggtcgc tgcggcaatc
     4621 gagcgcgcgc gtgacgcggg gcggacgctg cgggtcgccg gcgcagggca ctcgttcacc
     4681 gacgcggcgc tgaccgacgg gacgctgctg cggctcgacc gcatgcggcg ggtgctcgac
     4741 gtcgaccgcg ccagcgggct ggcacgcgtc gaggcgggca tctcgctgcg cgagctgagc
     4801 gagacgctgt gggcgcacgg gctggcgttc gagaacctcg gcgacatcga cgtgcagtcg
     4861 atcgccgggg cgacggcgac cggcacgcac gggaccggcg cgcggctgcg caacctctcg
     4921 gcggcgctgc gcgaggtcga gctcgtgacg gccgacggca ccaccgctcg cgtgtccgag
     4981 gacgccgatc ccgacgcctg gcgggcggcc cggatcggcg tcggcgcgct cggcgtcgta
     5041 acggcggtca ccctgcaggc cgtcccggcg ttcgtcctcg agggcgtcga ctcgaccgcg
     5101 ccgctcgagg agctgctcga cgggctcgac gagcgggccg acggcgccga ccacttcgag
     5161 ctctatgcct tcccgcacag ccccgtggcg gtgacgcgaa ccaaccgacg tgccgacagg
     5221 ccgcccgcgc cgccctcgcg cctgagcgcg tgggcgggcg acgtgctgct gcgcaactac
     5281 gtgctcggag cggccttcga gctcgggcgc cgtgcgccgc gctcgatccc gcggatgcac
     5341 cgggcgctca cgagcatcgc cgggacgacg cggcgggtcg atcgctccta ccgggtcttc
     5401 gcatcgccgc ggctggtgcg cttcacggag atggagtacg ccgtgccgcg tgcccgggcc
     5461 gccgatgccg tgcgggccgt gctcgccgtc gctcgccgcg cggacctggc ggtgccgatg
     5521 ccgatcgagg tccgcttcgt cgctcccgac gacgccttcc tgagcccagc cgggggacgt
     5581 gagacgtgct acgtcgcggt gcacatgtac cgcggcatgg agtgggcttc gtacttccgc
     5641 gcgatcgagg aggtcatgga cggcttcgag ggccgccccc actggggcaa gcgccacttc
     5701 cagaccgcgg ccacgctcgg tccgcgctat tcggagtggg agcgcttcca ggccgtgcgc
     5761 cggcgactcg accccgaggg cctgttcgcc aacggctacg tgcggcgggt gctcggggcc
     5821 tgagcacccg ggcgtcgccg gctgggtagg gtcgggggac aacgacgacg gaggcttcga
     5881 atggctgtct tggcgggatc ctcgagcgcg gagatcgatg cgccgctcga gcaggtgtgg
     5941 aaggccgtcg aggacgtcga gaaggcgccg gactggcagg gcggcatgaa ggacatcgag
     6001 gcgctcgagc gcgacggcga gggacgcgcg gtgctcgtcg agaccgaggc ggacgccaag
     6061 gtgacgacga tcaagacgaa ggtccgcttc agctacgacg gcccgacccg gctttcgtgg
     6121 aagcaggaga agggggacct gaagtcgatg gtcggctcct gggacctcga ggacctcggc
     6181 gacggccgta cgcgcgcgac ctacatgctc gagggcgaca ccggacgcat gctcggcatg
     6241 ttgatccgcg ggccggtcga ggacaagctg cgcgacttcc tggtcaacgc gcgcgccgac
     6301 gagctcaagg cgtacgtcga gggcagctag aggcgcacgc ggcgcacgca agcgccggtg
     6361 ctttgggaag ccctggcgtg gcgcaggcag gcggcgtcgg tgcgcttgcc gcggaaggcg
     6421 aaggtacggc tgacgccggt tgccgctgct cgctcggtga ccgtggcgct tcccttccag
     6481 gccagggctc cacgtctcgc ggtgacgcga cagcgcaccc ggacgacgct cagctgacgg
     6541 cagcgccgga cgccgatctt cgcgcgtctg ccgacgcgcc cgcgcatgcc gcggcgcatc
     6601 gccgaccgcg cctcggcgag cgtcagcgcg gcgatcggcg cggccggccc gccggtgcc
//
LOCUS       DATABV010000021         6836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1694088, whole genome shotgun sequence.
ACCESSION   DATABV010000021 DATABV010000000
VERSION     DATABV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694088"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VHF88_01040"
     CDS             complement(<1..1380)
                     /locus_tag="VHF88_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2160392.1"
                     /translation="MSAAARTPKKLVLAVIDALRPAALDRAIADDRAPVLAELVRRGE
                     YSRDCISTFPSLTPVAASAITTGLGPDEHLIPSMNWYHRGEERYVEYGSSFPATRAFG
                     IFRSLQDTVYNMNLAHLTQDHRTIFEQLDDAGLRTACTTYLIYRGRFRHEPAGDSRYR
                     RLAEAAQFRHAVWGARELFYADLFDSRHTGCSSTLGMPGQRDRHAGCVGAQLVEQDLF
                     DFLLLSLPDNDTYSHRAGPDAQPTSIAAADRALEQVFHAAGGVDEFLADHAVIVMSDH
                     SQSHVEQPVNLSHHLGDWRVLTPSDSAPEEAELAVCPGARSAMVYALDEGRRDRILPR
                     LVRQLRGIEGVDLTIWRDTGSDARAGDEAVVVALDGELRFAPADPLRAGERSNGRLSV
                     RDPRGGGWEVDGDLAVLDMTVDAGEAAFGDYPDALGRLWSALHCPRAGDVLLSAAPGY
                     EFVDLGGSHH"
     gene            complement(1377..1943)
                     /locus_tag="VHF88_01045"
     CDS             complement(1377..1943)
                     /locus_tag="VHF88_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX2160393.1"
                     /translation="MDIGTGAGLAGSVGVRPFLPPLLSGALARGDIGIDFDGTDWSFL
                     ESPVFLFAILVLAVAWYMAERSGANRRVMGRAGLLLSATLGALLFAGALADGGHSGIP
                     GLVAGVACAALAYVAIFALLSRAARRLDGEAAGFLTVYADAAALVLAAVAIFVPPVSF
                     LALIAFAVVLFRGRAAADRKYAGLRILR"
     gene            1996..3384
                     /locus_tag="VHF88_01050"
     CDS             1996..3384
                     /locus_tag="VHF88_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX2160394.1"
                     /translation="MTIDDPPERSTRRDLERYAGLFSARTRVMTSSATRDLMAMSDRP
                     EVISFAGGLPDTSSFPPETFASMTARIADESCARALQYGPTEGLAETKGCIVDVMAAE
                     QMTVDPGDVLVTTGGQQVLDLVTKTLIDPGDTIVAEGPSYPGALNAFQAYQANVVQID
                     MDADGMRVDLLEETLDRLERDGKRPKFVYTVPSFQNPGGVTMSLPRRKRLVEVARERE
                     LLVLEDNPYGLLRYEGEPLPTLYSLDGGVYVMYLGTFSKIFSPGLRLGWVVAPPPVLA
                     RINLGKQQADLCTSTLSQILVHAYFQHHDWREYVSALTELYRARRDTMLDALAEHFPP
                     QAEWTRPGGGLFIWATLPEFIDTSDLLAAALRDNVAFVPGEGAFLDGRGRSSMRLNFS
                     GADEGQIREGIRRIGEVVKEQVALYGTLTGEVAPEPARRGRDDEGDGRDEGGRVVELP
                     RRAERERRARRS"
     gene            3381..4319
                     /locus_tag="VHF88_01055"
     CDS             3381..4319
                     /locus_tag="VHF88_01055"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX2160395.1"
                     /translation="MSRVAVLAGGSSFERQVSLRSGARVEDALERLGHEVVAIDAGPD
                     LVRRLRDERLDAAFVAMHGRDGEDGTVQELLEILGLPYTGSGVPACARAMDKVLTKHL
                     LVEHGVPTPEFFAFNQTAFRELGAADALPAIEERLAFPIVVKPSSQGSALGIKFARTA
                     ADVPGALVAAFSYDAKVLLERFVDGRDLAVSVLDGPDGAVALPVVEAIPAGDVYDFQA
                     RYEIGQTEFVCPAELPDGVTERAQQLALRTYELLGCYGFGRVDLMLDAAGELYVLEAN
                     PIPGLTETSLLPQAAEEAGIGFDDLVDRILQSAFTR"
     gene            complement(4316..4804)
                     /locus_tag="VHF88_01060"
     CDS             complement(4316..4804)
                     /locus_tag="VHF88_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX2160396.1"
                     /translation="MQEMVIYGVSFDMVGKQPIVLLKTRDGNRFLPIWIGHPEAAAIL
                     MKLQGANTPRPMTHDLISDVLGELDVECTKVSVTELRENTFYASITLQLNGREMEIDS
                     RPSDALALAVRTGASIFAADDVITESAIEFEHEVEDTEEVVEKFKDFLDNVTPEDFQA
                     DS"
     gene            4862..4935
                     /locus_tag="VHF88_01065"
     tRNA            4862..4935
                     /locus_tag="VHF88_01065"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4896..4898,aa:Ile,seq:gat)
     gene            complement(4943..5794)
                     /locus_tag="VHF88_01070"
     CDS             complement(4943..5794)
                     /locus_tag="VHF88_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX2160397.1"
                     /translation="MGRGLSAILPTAKDETGLREIPVDLIEPNPRQPRREFDEEALVE
                     LSESIRSRGLLQPVVVRPLAGGKYELIAGERRLRASRLAELDAIPAVVRQTEDWERLD
                     LALAENMARVDLNPIEEARACSMLVDDLGLSKAEVGRRVGKSRVAVSNLVRLLELPDE
                     VLESIEAGDLSEAHGRAILTCKDHGDRKRLGRDAREQGWSVRETERRARQLEAGGPPE
                     RKAAEIHPDHAAAIAAVEETLSAALGRPVRARPKGDGCRVELDFESLADADELVQLLL
                     RSGLQAA"
     gene            complement(5829..6587)
                     /locus_tag="VHF88_01075"
     CDS             complement(5829..6587)
                     /locus_tag="VHF88_01075"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2160398.1"
                     /translation="MGTVFAIANQKGGVGKTTTAVNVAASIAEAGYETLLVDLDPQCN
                     ATVGVGLAKDLEPNAYTCLSGDLTIAEAAQPTPFERLSAVPAIPDLAGATVELPRLDG
                     SETRLREQLGDVRDRFLFTILDCPPSLGPLTVNALVAADRVLVPVQTEYFALEGLAGL
                     LDTLQLIQRELNPTLTVAGMVLTMHDSRTRLAQDVEREVREHFPDLVFDTVIPRNVRV
                     GEAPSYGMPVVRHAPRSAGSAAYLKLAKEVAARA"
BASE COUNT         1011 a         2345 c         2483 g          997 t
ORIGIN      
        1 gtgatgcgac ccgcccaagt cgacgaactc gtagccgggc gccgccgaca gcagcacgtc
       61 accggcgcgc gggcagtgca gcgccgacca gagccggccg agcgcatccg ggtagtcgcc
      121 gaaggcggcc tcgccagcgt cgaccgtcat gtcgaggacg gcgaggtcgc cgtcgacctc
      181 ccagccgccg ccgcgcggat cgcgcacgga caggcggccg ttcgaccgct cccccgcccg
      241 cagcgggtcg gcgggcgcga agcgcagctc gccgtcgagc gccacgacga ccgcctcgtc
      301 gcctgcgcgg gcgtccgacc ccgtatcgcg ccagatcgtc aggtcgacgc cctcgatccc
      361 gcgcagctgc cgaaccaggc gcggcaggat ccggtcgcgg cggccctcgt cgagcgcata
      421 gaccatcgcc gagcgcgctc ccggacagac cgccagctcg gcctcctcgg gggcgctgtc
      481 gctcggcgtc agcacacgcc agtcgccgag atggtgggaa aggttgacgg gctgctcgac
      541 gtggctctgc gaatggtcgg acatcacgat caccgcgtgg tcggcgagga actcgtcgac
      601 gccgccggcc gcgtggaaga cctgctcgag cgcgcggtcg gccgcggcga tcgaggtcgg
      661 ctgggcgtcg gggcctgcac gatgcgagta ggtgtcgttg tcgggcagcg acagcagcag
      721 gaagtcgaac aggtcctgct cgacgagctg ggcgccgacg cagccggcgt ggcggtcgcg
      781 ctggccgggc atcccgagcg tcgacgagca gccggtgtgg cgcgagtcga acaggtcggc
      841 gtagaacagc tcgcgcgcgc cccagacggc gtggcggaac tgcgccgcct cggcgagccg
      901 gcggtagcgc gagtcgccgg ccggctcgtg gcgaaagcgc ccgcgataga tcaggtacgt
      961 ggtacaggcg gtgcgcaatc cggcgtcgtc gagctgctcg aagatcgtcc ggtggtcctg
     1021 ggtcaggtgc gccaggttca tgttgtagac ggtgtcctgg agcgagcgga agatgccgaa
     1081 cgcgcgcgtg gcagggaacg aggacccgta ctcgacgtaa cgctcctcgc cacggtggta
     1141 ccagttcatc gacgggatca ggtgctcgtc ggggccgagg ccggtcgtga tcgccgacgc
     1201 ggcaaccggc gtcagcgacg gaaaggtcga gatgcagtcg cgcgagtact ccccgcggcg
     1261 gaccagctcg gcgagcaccg gggcgcggtc gtcggcgatc gcgcggtcga gcgccgcggg
     1321 cctgagcgcg tcgatgaccg cgaggacgag cttcttgggc gttctcgcag cggcgctcac
     1381 cgcaggattc gcagaccggc gtacttgcgg tcggcggcgg cacggcccct gaagagcacc
     1441 acggcgaacg cgatcaacgc cagaaacgag accggcggca cgaagatcgc caccgcggcg
     1501 aggacgagcg ccgcggcgtc ggcgtacacg gtgaggaagc cggccgcctc gccgtcgagg
     1561 cggcgggcgg cacggctgag cagcgcgaag atcgcgacgt aggcgagcgc ggcgcaggcg
     1621 acacccgcga cgaggccggg gatgcccgag tggccgccgt cagccagcgc gccggcgaag
     1681 agcagtgcac cgagcgtggc gctcagcaac agccccgccc tccccatcac gcgacggttc
     1741 gcgcccgaac gttcggccat gtaccaagcc accgcgagca cgaggatcgc gaacaggaac
     1801 acggggctct cgaggaagct ccagtcggtg ccgtcgaagt cgatgccgat gtcgccgcgc
     1861 gccagggcgc cgctgagcag cggcggcagg aacggccgca cgccgaccga gccggcgagg
     1921 ccggcgccgg ttccgatgtc catgacgagg ctgattggct ttgggctgtg ttgcggggac
     1981 ggtacgctgg agcgcgtgac gatagacgac ccgcctgagc gctcgacgcg ccgcgacctc
     2041 gagcgctacg ccggtctctt ctcggcgcgc acgcgcgtca tgacctcctc ggcgacgcgc
     2101 gacctgatgg cgatgagcga ccggccggag gtgatctcgt tcgccggcgg cctgcccgac
     2161 acctcgagct tcccgcccga gaccttcgcc tcgatgaccg cgcggatcgc cgacgagtcg
     2221 tgcgcgcggg cgctgcagta cgggccgacg gagggcctcg ccgagacgaa gggctgcatc
     2281 gtcgacgtga tggccgccga gcagatgacc gtcgaccccg gcgacgtgct cgtcacgact
     2341 ggcggtcagc aggtgctcga cctcgtcacc aagaccctga tcgatcccgg cgacacgatc
     2401 gtcgccgagg gcccctccta cccgggggcg ctgaacgcct tccaggccta ccaggcgaac
     2461 gtcgtccaga tcgacatgga tgccgacggg atgcgcgtcg acctgctcga ggagacgctc
     2521 gaccgcctgg agcgcgacgg caagcggccg aagttcgtct acacggtgcc gagcttccag
     2581 aaccccggcg gcgtaacgat gtcgctgccg cggcgcaagc gcctggtcga ggtcgcgcgc
     2641 gagcgcgagc tgctcgtgct cgaggacaac ccgtatggac tgctgcgcta cgagggcgag
     2701 ccgctgccga cgctctactc gctcgacggc ggcgtctacg tgatgtacct cggcacgttc
     2761 tcgaagatct tctcgcccgg gctgcggctc ggctgggtcg tcgcgccgcc gccggtcctg
     2821 gctcggatca acctcggcaa gcagcaggcc gacctctgca cctcgacgct gtcgcagatc
     2881 ctcgtgcacg cgtacttcca acaccacgac tggcgcgagt acgtcagcgc gctgaccgag
     2941 ctctaccgcg cacggcgcga cacgatgctc gacgcgctcg ccgagcactt cccgccgcag
     3001 gcggagtgga cgcggccggg cggcggactg ttcatctggg cgacgctgcc ggagttcatc
     3061 gacacctccg acctgctcgc ggcggcgctg cgcgacaacg tcgccttcgt gcccggcgag
     3121 ggcgcgtttc tcgacggccg cggccgcagc tcgatgcggc tcaacttctc cggcgccgac
     3181 gagggccaga tccgcgaggg cattcgccgg atcggcgaag tcgtcaagga gcaggtcgcg
     3241 ctctacggga cgctgaccgg cgaggttgcc ccggagccgg ctcgtcgagg gcgcgacgac
     3301 gagggcgacg gccgcgatga gggcgggcgg gtcgtcgagc tgccccgccg cgcggagcgc
     3361 gagcgccgcg cccgccgctc gtgagcaggg tcgccgtcct cgcgggcggc agctccttcg
     3421 agcgccaggt gtcgttgcgg tctggagcgc gcgtggagga cgcgctggag cggctcggcc
     3481 acgaggtcgt cgcgatcgac gccggcccgg acctggtccg ccggctgcgt gacgagcgcc
     3541 tcgatgccgc gttcgtcgcg atgcacggcc gcgacggcga ggacggcacc gtccaggagc
     3601 tgctcgagat cctcggcctg ccgtacacgg gctccggcgt gccggcctgc gcgcgggcga
     3661 tggacaaggt gctgaccaag cacctgctcg tcgagcacgg cgtgccgacg ccggagttct
     3721 tcgccttcaa ccagaccgcg tttcgcgagc tcggggcggc cgacgcgctg ccggcgatcg
     3781 aggagcggct ggccttcccg atcgtcgtca agccgtcgag ccagggctcc gcgctcggga
     3841 tcaagttcgc gcgtaccgcg gccgacgtgc cgggcgcgct cgtggcggcc ttctcctacg
     3901 acgccaaggt gctgctcgag cgcttcgtcg acggtcgcga cctcgccgtg tcggtgctcg
     3961 acggcccgga cggtgcggtc gcgctgcctg tcgtcgaggc gatcccagcc ggggacgtct
     4021 acgacttcca ggcgcgctac gagatcggcc agacagagtt cgtctgcccc gccgagctgc
     4081 cggacggcgt caccgagcgc gcgcagcagc tcgcgctgcg cacctacgag ctgctcggct
     4141 gctacggctt cggcagggtc gacctgatgc tcgacgccgc cggcgagctc tacgtcctcg
     4201 aggccaaccc gatccccggc ctgaccgaga ccagcctgct cccccaggcc gccgaggagg
     4261 ccggcatcgg cttcgacgac ctcgtcgacc gaatcctcca gtcggccttt actcgctagc
     4321 tgtccgcctg gaaatcctca ggcgtcacgt tgtcgaggaa atccttgaac ttctcgacga
     4381 cctcctcggt gtcctcgacc tcgtgctcga actcgatcgc ggactcggtg atcacgtcgt
     4441 cggcggcgaa gatcgaggca ccggtgcgca cggcgagggc gagcgcatcc gacggccgcg
     4501 agtcgatctc catctcgcga ccgttgagct gcagcgtgat cgaggcgtag aaggtgttct
     4561 cgcgcagctc ggtgaccgag accttggtgc actcgacgtc gagctcgccg agcacgtcgc
     4621 tgatcaggtc gtgggtcatc ggccgcggcg tgttcgcgcc ctgcagcttc atcaggatcg
     4681 ccgcagcctc gggatggccg atccagatcg gcaggaacct gttcccatcg cgcgtcttga
     4741 gcagcacgat cggctgcttg ccgaccatgt cgaagctgac gccgtagatg accatctcct
     4801 gcatgcgacc gaagtctaga cgcgccggtc gctggccggt ctcgctagaa tcgccgaccc
     4861 cgggcgatta gctcagtcgg ttagagcgcg tctctgataa ggacgaggtc cctggttcga
     4921 atccaggatc gcccactcgg gtctaggcgg cttgaaggcc gctgcgaagc aggagctgga
     4981 ccaactcgtc agcgtccgcg agcgactcga agtcgagctc gacgcggcac ccgtcgccct
     5041 tcggcctcgc ccgcaccggc cggccgagcg cggcggagag cgtctcctcg acggcggcga
     5101 tcgccgccgc gtggtcggga tgaatctcgg ccgccttgcg ctccggcggg ccgccggcct
     5161 cgagctgtcg cgcacggcgc tctgtctcgc ggaccgacca gccctgctca cgcgcgtcgc
     5221 gcccgaggcg ttttcggtcc ccatggtcct tgcaggtcag gatcgcccgg ccgtgagcct
     5281 cgctgaggtc gccggcctcg atcgactcga gcacctcgtc cgggagctcg agcaggcgca
     5341 ccaggttcga caccgcgacc ctgctcttgc cgacacgccg gccgacctcc gccttcgaga
     5401 ggccgagatc atcgacgagc atcgagcagg cgcgtgcctc ctcgatcggg ttgaggtcga
     5461 cgcgcgccat gttctccgcg agcgccaggt cgagacgctc ccagtcctcg gtttggcgca
     5521 ccacagccgg gatcgcgtcg agctcggcga gccgagaggc gcgcaggcgc cgctcccccg
     5581 cgatcagctc gtacttcccg ccggcgagcg gacggacaac gacgggctgc agcaagccgc
     5641 gcgaacggat cgactcggac agctcgacca gggcctcctc gtcgaactca cgccgcggtt
     5701 ggcgtgggtt cggctcgatc aggtcgaccg ggatctcacg cagcccggtc tcgtccttgg
     5761 ctgtcggcaa gatcgccgac aggccgcgac ccatcccacg gcgcccagcc ttaggctcag
     5821 gctcacggtc aagcacgggc tgcaacctcc ttggcgagct tcaggtaagc ggccgatccg
     5881 gcgctccgag gggcatgcct gacgaccggc atcccatagc tcggggcctc tcccaccctg
     5941 acgttacgcg ggatcaccgt gtcgaagacg aggtcgggga aatgctcccg cacctcgcgc
     6001 tcgacatcct gcgccaggcg ggtccgactg tcatgcatgg tgagcaccat gcccgcaacc
     6061 gtaagggtcg ggttgagctc tcgctggatc aattgaaggg tgtcgagcag gccggcgagg
     6121 ccctcgaggg cgaaatactc ggtctggacg ggcacgagga cgcggtcggc ggcgacgagg
     6181 gcgttgacgg tcagcggacc gagcgagggc ggacagtcga ggatcgtgaa gaggaagcgg
     6241 tcgcggacgt ccccgagctg ctcgcggagg cgggtctccg agccgtcgag gcggggaagc
     6301 tcgacggtgg cgcctgcgag gtctgggatc gcgggaaccg cggagaggcg ctcgaatggg
     6361 gttggttggg cggcctcggc gatcgtgagg tcgcctgaaa ggcatgtgta ggcgttgggc
     6421 tctaggtcct tggccagtcc gacgccgacg gtggcgttgc actgggggtc gaggtcgacg
     6481 agcagggtct cgtagccggc ctcggcgatg ctggcggcga cgttgacggc ggtggtggtc
     6541 ttgccgacgc cgcctttttg gtttgcgatt gcgaagacgg ttcccacggg gtgagggtac
     6601 tggtgggggt ggtcggaggg gtgggtggtt cgggggcgaa cggttgtggt cgacggcttc
     6661 gggctcggcg tgggcggccg cggggcctgt ctccgatttt ggtttacgtg ctcgcaagct
     6721 gcgcgcgcaa acgcaaaatc gtcgccaccc gcggacgcag tcgtttactg ccgccggcct
     6781 agcgggcgct ttgtggccat gccgggacgg cgggggaagc gctctggcgt ggggga
//
LOCUS       DATABV010000022         5755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1694089, whole genome shotgun sequence.
ACCESSION   DATABV010000022 DATABV010000000
VERSION     DATABV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694089"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..492)
                     /locus_tag="VHF88_01080"
     CDS             complement(226..492)
                     /locus_tag="VHF88_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160399.1"
                     /translation="MPPRQPYRLRTRGDREVIREVEPGKDYVDSETGQPFEVVGKLIP
                     LAPSPSNLPYSVENLRMCGCSLEQLVQKDLNDCPHCGRRLPAHS"
     gene            547..2055
                     /locus_tag="VHF88_01085"
     CDS             547..2055
                     /locus_tag="VHF88_01085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2160400.1"
                     /translation="MAAVRVLVFHGYLLRGTGSNIYNLSLARALAALGHDVCLLCQER
                     GHGDLDLPAGVTVVNPEIGRTLPVYVADSYAGFDAVRFADLDEAALERYLDANVAAVR
                     AAVEDFGPDVALANHAVMGPAILARGLAGRVPYAVKIHGSALEYTVRPQPERFLPYML
                     EGLRGAAGVLVGSRHTAESLWEVAGDPALPAKTRLGPPGVDVAAFRPRPAAEAADGLT
                     ELADRLEGAESSGWGGEPGAAAALRAFDPRRERIVGYVGKLIVSKGVDLLIAAWPLVS
                     ARAPDARLLIVGFGAYRDTLGELVAALGRADLAAVRAIAAQGRAPEGGPVSELTYLAA
                     FLDGLEGAARDAYLAAAPEAARRVRFSGSLEHGDLPLVLPSFSAQVVPSTFPEAFGMV
                     AAEAACCGALPISADHSGLAEVTSALADALPEERRPLLSFDRGPAAVEQIADRLIRWL
                     ELSAEQRAAAAGALSSGARARFGWENVAEGVIAAAQGRLDLLPVVPSGVPSG"
     gene            2067..2783
                     /locus_tag="VHF88_01090"
     CDS             2067..2783
                     /locus_tag="VHF88_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX2160401.1"
                     /translation="MRSGSQRLAATVAIAASVVVVAAGCGRGDPDLVAGKQLFVEKCG
                     ACHTLARAGTSGVNGPNLDEAFGPARRHGLGAGTVEGVTADQIANPLRNSGMPAKLVT
                     GEDADDVAAYVAAVAGQPGEDQGRLAAAGTPEVSNEPIAAEGGRLEIDANPTGALAFE
                     STKAQAPPGPIEILSMNESPIPHNIALEQPGGKVVEGPVVETGGTSRLQADLEPGEYE
                     FVCTVPGHEEGGMKGTLTVK"
     gene            complement(2776..3579)
                     /locus_tag="VHF88_01095"
     CDS             complement(2776..3579)
                     /locus_tag="VHF88_01095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2160402.1"
                     /translation="MSDPTDAGAPAGGDLELAERVARAAGALLLDRYEGPARGLAEKS
                     SRTDMVSDADRDAEALIRELLAAERPEDGLLGEEGSLVEGSSGRRWVVDPLDGTTNYL
                     YRFPAWVVSVALEDGDASSGTGGALVGVVYDPLRDEAFTAARGGGARLNGAPIAVSGV
                     ARLDSALIGTGFGYAAERRAEQAAVAARLLPLVRDIRRAGAAALDLCMVACGRLDGYY
                     ERGLNVWDWAAGSLIAKEAGATVRPLPGEPFGLTVAPPAIADELAQLAT"
     gene            complement(3683..4330)
                     /locus_tag="VHF88_01100"
     CDS             complement(3683..4330)
                     /locus_tag="VHF88_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX2160403.1"
                     /translation="MESASIPAAGAQTPTSPPHEHEAHEHHGPPAANQSSRLDPQFLG
                     MLLFIISEVMLFGAFFTAYFFIRVVNGEPWPAEGDHLPVAIAGVNTAILVSSSFTMHW
                     ALEGARRENRRALRIGLLTTLLLGTTFLCIQINEYFHIGFAVSENAQSSIFYGLTGLH
                     GAHVFVGLTLLAFATIRAFRGHFSAKKHLGVEIPGIYWHFVDVMWIVVYTTVYII"
     gene            complement(4330..5166)
                     /locus_tag="VHF88_01105"
     CDS             complement(4330..5166)
                     /locus_tag="VHF88_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2160404.1"
                     /translation="MSEPRNILVVANETVTGQPLIDAVKRRADGSAVSVHVICPQNQP
                     KHGYVIYDDTVRGAAENRLAMTLAQLREVGIEATGDVEDPDPYSAIMDAVGERDYDEI
                     VISTHPDTRSGWLRQDLITRVEQATRRPVEHVVVDLGPHDPDEGTRTLVVANQTVGGQ
                     PLIDQLKELAAEGPRRFIVVCPQGGAGENDAHRRLAHTLKRLQDEGLEAIGQVTHPDP
                     YTAIQQALHFYGVDEVVISTFQATRSGWLRSDLLRRVQASTDKPVHHVVVSSEQVEQA
                     EA"
     gene            complement(5163..>5755)
                     /locus_tag="VHF88_01110"
     CDS             complement(5163..>5755)
                     /locus_tag="VHF88_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2160405.1"
                     /translation="MLAVVPIDIHVSDTYFVVAHIHYVLFGGSVFTIFAGIYHWFPKI
                     TGRMYDEGLGKVHFWLSFVFFNLTFGPMHFVGVDGMPRRVADYADQFATLNAIISVSA
                     FLFGLSFLIFLYNMVTSWRFGPKASGNPWRAHSIEWQVSSPPPIFNFDEVPTVVGGPY
                     EYGVPGAVHAVFADKKAPVGATPAPAGGGAPPPNEP"
BASE COUNT          823 a         2033 c         2126 g          773 t
ORIGIN      
        1 cgcggccgct ggcgccgccg cgacggccat ccgccgacgc tgcgccgtcc tggtcggctg
       61 gggcgacccg tcccgcagcc gctcccagcc cgagtccgat ccccagccac acctcggcgc
      121 gctcctagcc gctcgaaaac cgagcatggc aagccggcgg ccgagccata gggcgtccgc
      181 ccatggctcc gccgccggcg ccgccaggcg aaggtttgca gccggctagg agtgggcggg
      241 cagcctgcgc ccgcagtgag gacagtcgtt caagtccttc tggaccagct gctcgagcga
      301 gcagccgcac atgcgcaggt tctcgaccga gtaggggagg ttgctgggcg acggagcgag
      361 cgggatcagc ttgccgacga cctcgaacgg ctgaccggtc tcgctgtcga cgtagtcctt
      421 gcccggctcg acctcgcgga tgacctcgcg atcgccgcgc gtgcggaggc ggtacggctg
      481 acggggcggc atggcgcggc agactagcaa gcggccagcg acgtcgatcg cgcggctcct
      541 gcgagcatgg cggccgtgcg cgtcctggtc ttccacggct acctgctgcg cgggaccggc
      601 agcaacatct acaacctgag cctggcgcgc gcgctggccg cgctcgggca cgacgtctgc
      661 ctgctgtgcc aggagcgggg gcacggcgac ctcgatctgc cggccggcgt gacggtcgtc
      721 aaccccgaga tcggccgcac gctgccggtc tacgtggcgg acagctacgc ggggttcgac
      781 gccgtccgct tcgccgatct cgacgaggcg gcgctcgagc gctatctcga cgccaacgtc
      841 gccgcggtcc gtgcggcggt cgaggacttc gggcccgacg tcgcgctggc caaccatgcc
      901 gtgatgggcc cggcgatcct cgcgcgcggc ctcgccgggc gggtgcccta cgccgtgaag
      961 atccacggca gcgcgctcga gtacacggtc cgaccgcagc ccgagcgctt cctgccctac
     1021 atgctcgagg gcctgcgcgg cgcggccggg gtgctcgtcg gctcgcgtca caccgcagag
     1081 agcctctggg aggtcgccgg cgaccccgcg ctgccggcca agacccgcct cggcccgccg
     1141 ggcgtcgacg tggctgcctt ccgaccgcgc ccggccgcgg aggccgcgga cgggctgacc
     1201 gagctggccg accgcctgga gggagccgag agcagcggct ggggtggcga gccgggcgcc
     1261 gccgcggcgc tgcgcgcctt cgaccctcgc cgggagcgga tcgtcggcta cgtcggaaag
     1321 ctgatcgtct cgaagggagt cgacctgctg atcgccgcct ggccgctcgt cagcgcacgg
     1381 gcgccggacg cgcgcctgct gatcgtcggc ttcggcgcct accgcgacac gctcggcgag
     1441 ttggtcgccg cgctcgggcg cgccgatctc gcggctgtcc gcgcgatcgc cgcacagggt
     1501 cgcgccccgg agggcggccc ggtgtccgag ctgacctacc tggcggcgtt cctcgacggc
     1561 ctcgagggag ccgcccgcga cgcctacctc gcggcggcgc ctgaggcggc ccggcgcgtg
     1621 cgcttcagcg gaagcctcga gcacggcgac ctgccgctcg tgctgccgtc gttcagcgcg
     1681 caggtcgtcc ccagcacctt cccagaggca ttcgggatgg tcgccgcgga ggcggcctgt
     1741 tgcggggcgc tgccgatctc cgccgaccac tccgggctcg cggaggtcac gtcggcgctc
     1801 gccgacgccc tgccggagga gcggcgtccg ctgctctcct tcgatcgcgg tccggcggcg
     1861 gtcgagcaga tcgcggaccg gctgatccgc tggctcgagc tgtccgccga gcagcgcgct
     1921 gcggccgccg gcgcgctgtc gtccggcgct cgcgcgcgct tcgggtggga gaacgtggcg
     1981 gagggcgtga tcgcggccgc gcaggggcgg ctcgacctgc tccctgtcgt gccttccggc
     2041 gtgccgagcg ggtagagttc cggcccgtga ggagcggatc gcagaggctc gccgccacgg
     2101 tggcgatcgc ggcgtcggtc gtcgtcgtcg cagcgggctg cggccgcggg gatcccgatc
     2161 tcgtcgcggg caagcagctc ttcgtcgaga agtgcggcgc ctgccacacg ctcgcgcggg
     2221 cgggcaccag cggcgtcaac gggcccaacc tcgacgaggc cttcggtccg gcacgccgcc
     2281 acggcctcgg cgcgggcacg gtcgagggcg tcaccgccga ccagatcgcg aacccgctgc
     2341 gcaacagcgg catgcctgcc aagctcgtca ccggcgagga cgccgacgac gtcgccgcct
     2401 acgtggcggc ggtcgccggc caacccggcg aggaccaggg ccggctcgcc gcggccggga
     2461 cgccggaggt ctcgaacgag ccgatcgccg ccgagggcgg ccggctcgag atcgacgcca
     2521 acccgaccgg cgcgctcgcg ttcgagagca cgaaggcgca ggctccgccc ggcccgatcg
     2581 agatcctcag catgaacgag tcgccgatcc cgcacaacat cgcgctcgag cagcctggcg
     2641 ggaaggtcgt cgagggaccg gtcgtcgaga ccggcgggac ctccaggctg caggccgacc
     2701 tggagcccgg cgagtacgag ttcgtctgca ccgtcccggg gcacgaggag ggcggcatga
     2761 agggaacgct gacagtcaag tagcgagctg agctagctcg tcggcgatcg cgggaggggc
     2821 gacggtgagc ccgaagggct cgcccggcag gggacggacg gtcgccccag cctcctttgc
     2881 gatcagcgag ccggcggccc agtcccagac gttgaggccg cgctcgtagt agccgtcgag
     2941 ccggccgcag gcaaccatgc agaggtcgag cgcggctgcg cccgcgcggc ggatgtcgcg
     3001 cacgagcggg aggagccggg ccgcgaccgc cgcctgctcc gcgcgccgct cggccgcata
     3061 gccgaagccg gtgccgatca gcgcgctgtc gagccgcgcc acgccgctga cggcgatcgg
     3121 ggcgccgttc aggcgcgctc cgccgccgcg cgccgcggtg aaggcctcgt cgcgcagcgg
     3181 gtcgtacacg acgccgacca gggcgccacc cgtgccgctc gaggcgtccc cgtcctcgag
     3241 cgccacgctg acgacccagg cggggaaccg gtagaggtag ttggtcgtgc cgtcgagcgg
     3301 gtcgaccacc cagcgccgtc cgctgctgcc ctcgacgagc gagccctcct cgccgagcag
     3361 cccgtcctcg ggacgctcgg ccgcgagcag ctcgcggatc agcgcctcgg cgtcgcggtc
     3421 ggcgtcggag accatgtcgg tccggctcga cttctccgcg aggccgcgcg caggcccctc
     3481 gtagcggtcg agcagcagcg cgccggccgc ccgcgcgacg cgctcggcaa gctcgaggtc
     3541 gccccctgcg ggggcaccgg cgtcggtcgg atcgctcaga gggcgcgtac cacgaggacg
     3601 acggccacca ccacgagcgc gaccgccccg acgtagagca gcagctggaa ggcgccctgc
     3661 tccgaggtca gcgggttgcg cactagatga tgtagacggt cgtgtagacc acgatccaca
     3721 tcacgtcgac gaagtgccag tagatcccgg ggatctccac gccgaggtgc ttcttggccg
     3781 agaagtgccc acggaaggcg cgaatcgtcg cgaacgcgag cagcgtcagg ccaacgaaga
     3841 cgtgggcccc gtgcaggccc gtcaggccgt agaagatcga cgactgggcg ttctccgaga
     3901 ccgcgaagcc gatgtggaag tactcgttga tctggatgca gaggaacgtc gtgccgagca
     3961 gcagcgtggt cagcagaccg atccgcagtg cgcggcggtt ctcgcgccgc gctccctcga
     4021 gcgcccagtg catcgtgaag ctcgacgaga cgaggatcgc cgtgttgacg cccgcgatcg
     4081 ccaccggcag gtggtcgccc tccgccggcc acggctcgcc gttgacgacc cgaatgaaga
     4141 agtaggccgt gaagaacgct ccgaagagca tcacctcgga gatgatgaac agcagcatcc
     4201 cgaggaactg cgggtcgagc cgcgacgact ggttcgccgc cggcgggccg tgatgctcgt
     4261 gtgcctcgtg ctcgtgcggc ggggacgtcg gagtctgcgc gccggcggcc gggatcgaag
     4321 cggactccat caggcctccg cctgctcgac ctgctcgctg gacacgacga cgtgatggac
     4381 cggcttgtcg gtcgacgcct ggacccgccg cagcaggtcg gagcgcagcc agcccgagcg
     4441 cgtcgcctgg aaggtcgaga tcacgacctc gtcgacgccg tagaagtgaa gcgcctgctg
     4501 gatcgccgta tacgggtccg ggtgggtcac ctggccgatc gcctcgagcc cctcgtcctg
     4561 gagccgcttg agcgtatgcg ccaggcggcg atgagcgtcg ttctcaccgg cgccgccctg
     4621 cgggcagacg acgatgaagc ggcgcggccc ctcggcggcg agctccttga gttggtcgat
     4681 cagcggctgg ccgccgacgg tctggttcgc gacgacgagc gtgcgcgtcc cctcgtcggg
     4741 gtcgtgtggg ccgaggtcga cgacgacgtg ctcgacagga cggcgggtcg cctgctcgac
     4801 acgcgtgatc agatcctggc gcagccagcc cgagcgggtg tcgggatgcg tcgagatgac
     4861 gatctcgtcg tagtcgcgct cgccgaccgc gtccatgatc gcggagtagg ggtcgggatc
     4921 ctcgacgtcg ccggtcgcct cgatgccgac ctcgcgcagc tgggctagcg tcatcgcgag
     4981 ccggttctcg gccgcgccgc ggaccgtgtc gtcgtagatc acgtagccgt gcttcggctg
     5041 gttctgcggg cagatcacgt gcaccgatac ggcactgccg tcggcgcgcc gcttgacggc
     5101 gtcgatcagc ggctgaccgg tgacggtctc gttcgcgacg acgaggatgt tgcgtggctc
     5161 gctcatggct cgttcggggg cggggccccg ccgccggccg gggccggcgt cgcgccgacc
     5221 ggcgccttct tgtcggcgaa caccgcgtgc accgcaccgg ggacgccgta ctcgtaggga
     5281 ccgccgacga ccgtcgggac ctcgtcgaag ttgaagatcg gcggcggcga ggacacctgc
     5341 cactcgatcg agtgggcgcg ccacgggttg cccgaggcct tcgggccgaa gcgccagctc
     5401 gtgaccatgt tgtagaggaa gatcaggaac gagaggccga acaggaacgc cgacaccgag
     5461 atgatcgcgt tgagcgtcgc gaactggtcg gcgtagtcgg ccacccggcg cggcatcccg
     5521 tcgacgccga cgaagtgcat cgggccgaag gtcaggttga agaagacgaa cgaaagccag
     5581 aagtggacct tgccgagccc ctcgtcgtac atccggccgg tgatcttcgg gaaccagtgg
     5641 tagatgccgg cgaagatcgt gaacaccgag ccgccgaaca gcacgtagtg gatgtgcgcg
     5701 acgacgaagt aggtgtccga cacgtggatg tcgatcggca cgacggcgag catga
//
LOCUS       DATABV010000023         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_328822, whole genome shotgun sequence.
ACCESSION   DATABV010000023 DATABV010000000
VERSION     DATABV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328822"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VHF88_01115"
     CDS             complement(<1..530)
                     /locus_tag="VHF88_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2160406.1"
                     /translation="MATDVLMPRLSDSMEEGTVLKWLVEKGGAVKRGAPLVEIETDKA
                     NMTYEADTDGVLIELLAQEGDTLEVGQPIARIGAAGEAGGAAADEEAKAEGETPAEAD
                     DDASAADDAPVDEPAQATDDAGQEPAKAAPPAPVAKANGGERVKASPLARRMAGELGV
                     ELARVEGSGPGGRVVKA"
     gene            complement(530..1555)
                     /locus_tag="VHF88_01120"
     CDS             complement(530..1555)
                     /locus_tag="VHF88_01120"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2160407.1"
                     /translation="MASAATSAGQAPAGVTKMRYREALNLALREEMLADERVFLIGED
                     VGVFQGAFKVTAGLLEEFGERRVRDTPISENTIVGMGVGAAMAGLRPVVELMTINFSL
                     LALDQIVNSAASIRYMFGAQVKVPMVIRMPQGAGHQLGPTHSHSFEALYQHVPGLLVA
                     VPTTAADARGLLKAAIRDDNPVVFIEHETLYGRRGDVPDEQEPLRFGEAAVRREGGDV
                     TIVGISRMAETALKAADTLASEHQIEAEVIDPRTLRPLDLDTILASVRKTNRAVIVEE
                     GWPHGGVGANMAALIQEQAFDHLDAPIARVTGADVPMPYSKPLEQAAFPHEEHVVQAA
                     RATLGDL"
     gene            complement(1555..2760)
                     /gene="pdhA"
                     /locus_tag="VHF88_01125"
     CDS             complement(1555..2760)
                     /gene="pdhA"
                     /locus_tag="VHF88_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX2160408.1"
                     /translation="MATEDATGGRGPDATGVEAATHPLPDRSVCEQVLARMQLIRRFE
                     ERAGEMYAKAKIGGFLHLCIGEEATIVGSTQALRDSDYLLSTYREHGHALARGTPPGA
                     VMAELFGKVDGCSGGRGGSMHLFDVERRLLGGYGIVGGNLPLAAGVALASDYTGTDDA
                     TLCMFGDGATNQGTFGETMNLAALWKLPVVFMVVNNQFGMGTALERHSAVTDLSRKAA
                     GYGVPGSRCDGMDVLSVERCVAAALEKARDGRQPQLVEAVTYRYRGHSMADPEEYRTK
                     DEVEDWRTRDPLKTFPARLVEEDVLSEEDVQRLDEQAIATVDEAVEFAERSPFPDLAS
                     LYDDIYVIGDQVRDPWWSTDARSPDVHRGEQEREAGDVAQELAEAGAAYADETDPDTR
                     AARARGAGG"
     gene            complement(2846..3364)
                     /locus_tag="VHF88_01130"
     CDS             complement(2846..3364)
                     /locus_tag="VHF88_01130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX2160409.1"
                     /translation="MRTRYFIACGLAALSLTAAGCGDETETAEFTVEAPPASESAAAE
                     QEPASSGKEPKVRVPDGPPPKQLQTRDIKEGSGQAAKTGDNVSMNYVGVSYSTKKKFD
                     SSYDRGQPIDVPLGQGQVIPGWDEGIVGMKVGGRRELIIPPDMAYGAQGRPPDIKPNE
                     TLVFVVDLVAIN"
     gene            complement(3375..3959)
                     /locus_tag="VHF88_01135"
     CDS             complement(3375..3959)
                     /locus_tag="VHF88_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX2160410.1"
                     /translation="MTDDDRPVFYYDLGSPYAWLAAERIHAVMPVMPVWQPILLGGIW
                     QETGGGSWSLTDARAEGMVEVERRAREYGLMEVRWPEQWPTNSLLAMRAATFAQQAGR
                     AVAFSLAAFRQAFCAGRDLADPDNVLIAAAACELHPNAIAKGVESRSVKDRLRAATAE
                     AAARGVIGVPTVAVGDELFWGDDRLEEAAAAAGS"
     gene            complement(3975..>4339)
                     /locus_tag="VHF88_01140"
     CDS             complement(3975..>4339)
                     /locus_tag="VHF88_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160411.1"
                     /translation="ARIRRARRAAQRRGRRPAWRDARRVTVAGGRPVLFHGRVGRPVP
                     RNGKLVEVQAHFRGRWRTISTVRSRRGGRWQFRYRFQAATRRATYRLRARAPVEAGYP
                     FAAGASRPVRVTVLPARR"
BASE COUNT          519 a         1644 c         1529 g          647 t
ORIGIN      
        1 gccttgacga cgcgtccgcc gggcccgctg ccctcgacgc gcgcgagctc gacgccgagc
       61 tcgccggcca tccgccgggc gagcggcgac gccttgaccc gctcgccgcc gttggccttg
      121 gcgaccggcg cgggcggcgc cgccttcgcg ggctcctgcc ctgcgtcgtc ggtcgcctgt
      181 gccggctcgt cgaccggggc gtcgtccgca gcgctcgcgt cgtcgtccgc ctcggcgggc
      241 gtctcgccct ccgccttcgc ctcctcgtcc gccgccgccc cgccggcctc gcccgccgcg
      301 ccgatccgcg cgatcggctg gccaacctcg agcgtgtcgc cctcttgggc gagcagctcg
      361 atcagcacgc cgtcggtgtc ggcctcgtag gtcatgttcg ccttgtcggt ctcgatctcg
      421 acgagcggcg cgccgcgctt gaccgcgccg cccttctcga cgagccactt caggacggtg
      481 ccctcctcca tcgagtcgga caggcgcggc atcaggacgt cggtcgccat cagaggtctc
      541 cgagcgtcgc gcgtgcggcc tggactacgt gctcctcgtg cgggaaggcg gcctgctcga
      601 gcggcttcga gtacggcatc ggcacgtcgg cgccggtgac gcgggcgatc ggcgcgtcga
      661 gatggtcgaa cgcctgctcc tggatcagcg ccgccatgtt ggcgccgacg ccaccgtgcg
      721 gccagccctc ctcgacgatc accgcgcggt tggtcttgcg gacggaggcg aggatcgtgt
      781 cgaggtcgag cgggcgcagc gtgcgcgggt cgatcacctc ggcctcgatc tggtgctccg
      841 atgccagcgt gtcggccgcc ttgagcgccg tctcggccat ccgcgagatc ccgacgatcg
      901 tcacgtcgcc gccttcgcgg cggaccgccg cctcaccgaa gcgcagcggc tcttgctcgt
      961 cgggcacgtc tccgcgccgg ccgtagagcg tctcgtgctc gatgaagacc accgggttgt
     1021 cgtcgcggat cgcggccttc agcaggccgc gcgcgtcggc ggcggtggtc gggacggcga
     1081 ccagcagccc gggcacgtgc tggtagagcg cctcgaagga gtgcgagtgg gtcgggccga
     1141 gctggtgccc ggcgccctgg ggcatccgga tcaccatcgg caccttgacc tgggcgccga
     1201 acatgtagcg gatcgaggcc gcgctgttga cgatctggtc gagcgcgagc agcgagaagt
     1261 tgatcgtcat cagctcgacg accggtcgca gccccgccat cgccgcgccg acgcccatcc
     1321 cgacgatcgt gttctccgag atcggcgtgt cgcgcacccg ccgctcgccg aactcctcga
     1381 gcaggccggc ggtcaccttg aacgctccct ggaagacgcc gacgtcctcg cctatcagga
     1441 agacgcgctc gtcggcgagc atctcctcgc gcagcgcgag gttgagcgcc tcgcggtagc
     1501 gcatcttcgt aaccccggca ggcgcctgcc cggcgctggt cgccgcgctc gccatcagcc
     1561 gcccgctccc cgcgcccgcg cggcgcgcgt gtccgggtcg gtctcgtccg cgtaggcggc
     1621 cccggcctcg gcgagctcct gcgcgacgtc gccggcctcg cgctcctgct cgccgcggtg
     1681 gacgtcgggc gagcgcgcgt cggtcgacca ccacgggtcg cgcacctggt cgccgatcac
     1741 gtagatgtcg tcgtagagcg aggcgaggtc ggggaacggc gagcgctcgg cgaactcgac
     1801 cgcctcgtcg acggtggcga tcgcctgctc gtcgagccgc tgcacgtcct cctcgctcag
     1861 cacgtcctcc tcgaccaggc gcgccgggaa ggtcttcagc gggtcgcgcg tgcgccagtc
     1921 ctcgacctcg tccttggtcc ggtactcctc cggatcggcc atcgagtggc cgcggtagcg
     1981 gtaggtgaca gcctcgacga gctgcggctg gcgcccgtcg cgcgccttct cgagcgccgc
     2041 cgccacgcag cgctcgacgc tcaggacgtc catgccgtcg cagcggctgc ccggcacgcc
     2101 gtagccggcc gccttgcgcg acaaatcggt gaccgccgag tgacgctcga gcgcggtgcc
     2161 catcccgaac tggttgttga cgaccatgaa cacgaccggc agcttccaga gcgcggcgag
     2221 gttcatcgtc tcgccgaacg tgccctggtt ggtcgcgccg tcgccgaaca tgcagagcgt
     2281 ggcgtcgtcg gtccccgtgt agtcggacgc gagcgcgacg ccggccgcta gcggcaggtt
     2341 tccgccgacg atcccgtagc cgccaagcag ccgccgctcc acgtcgaaga ggtgcatcga
     2401 tccgccgcgc ccgcccgagc agccgtcgac cttgccgaac agctccgcca tcaccgcgcc
     2461 gggcggcgtg ccgcgcgcca gcgcgtggcc gtgctcgcgg taggtcgaga gcaggtagtc
     2521 gctgtcgcgc agcgcctgcg tcgagccgac gatcgtcgcc tcctcgccga tgcagaggtg
     2581 caggaagccg ccgatcttcg ccttcgcgta catctcgccg gcgcgctcct cgaagcggcg
     2641 gatcagctgc atccgcgcga ggacctgctc gcagaccgat cggtcgggga gggggtgcgt
     2701 cgcggcctct acgcccgtcg cgtcagggcc gcggccgccg gtagcgtcct cggtcgccat
     2761 ctatccacac cctagcggcg ggcgcttctc tgccagatgg cgtgactccg gtttaccgac
     2821 ggtcggggga accgcctgcg ctcgctcagt tgatcgcgac gaggtccacg acgaagacga
     2881 gcgtctcgtt cggcttgatg tccggcggcc gcccctgcgc cccatacgcc atgtcgggcg
     2941 ggatgatcag ctcgcgccgg ccgccgacct tcatgccgac gatcccctcg tcccagccgg
     3001 ggatgacctg gccctggccg agcgggacgt cgatcggctg gccgcggtcg taggagctgt
     3061 cgaacttctt cttggtcgag tacgagacgc cgacgtagtt catcgacacg ttgtcgcccg
     3121 tcttggcggc ctggccgctg ccctccttga tgtcacgcgt ctggagctgc ttcggcggcg
     3181 gcccgtcggg cacccgcacc ttcggctcct tgccggagct ggccggctcc tgctcggccg
     3241 ccgcgctctc gcttgcgggc ggcgcctcga ctgtgaactc ggcggtctcg gtctcgtcgc
     3301 cgcagccggc ggcggtcagg gagagtgcgg cgagaccgca ggcgatgaag tatcgggtac
     3361 gcatgcggac catcctatga gccggcggcg gccgctgcct cctcgagccg gtcgtcgccc
     3421 cagaacagct cgtcgccgac tgcgacggtc ggcacgccga tcaccccgcg cgccgccgcc
     3481 tccgcggtcg cggcgcgcag gcggtccttg accgagcggc tctcgacgcc cttggcgatc
     3541 gcgttcggat gcagctcgca ggcggcggcg gcgatcagca cgttgtcggg atcggcgagg
     3601 tcgcgcccgg cgcagaacgc ctggcggaac gccgcgagcg agaacgccac ggcgcgcccg
     3661 gcctgctggg cgaaggtcgc cgcccgcatc gcgagcagcg agttggtcgg ccactgctcg
     3721 ggccagcgga cctccatcag cccgtactcg cgcgcccggc gctcgacctc caccatcccc
     3781 tcggcccggg cgtcggtcag cgaccacgat ccgccgcctg tctcctgcca gatcccgccg
     3841 agcaggatcg gctgccagac cggcatcacc ggcatgacgg cgtggatccg ctccgcggcc
     3901 agccaggcgt agggcgagcc gaggtcgtag tagaagacgg ggcggtcgtc gtcggtcatg
     3961 cgggcctcct cagttcagcg ccgggcgggc agcaccgtaa cgcggaccgg ccgcgatgcg
     4021 ccggccgcga acggatagcc ggcctcgacc ggtgcgcgtg cccgcagccg gtaggtcgcc
     4081 cgccgcgtcg ccgcctggaa gcggtagcgg aactgccagc gcccgccacg gcgcgagcgc
     4141 acggtggaga tcgtgcgcca gcggccgcgg aagtgcgcct gcacctcgac cagcttgccg
     4201 ttgcgcggga ccggccgccc gacgcggccg tggaacagga cgggccgccc gccggcgacc
     4261 gtcacccgtc gcgcatcgcg ccaggccggc cgccggccgc gccgctgcgc ggcgcgccgt
     4321 gcccgcctga tgcgcgccg
//
LOCUS       DATABV010000024         9438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2658175, whole genome shotgun sequence.
ACCESSION   DATABV010000024 DATABV010000000
VERSION     DATABV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658175"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..378
                     /locus_tag="VHF88_01145"
                     /pseudo
     CDS             1..378
                     /locus_tag="VHF88_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(466..1020)
                     /locus_tag="VHF88_01150"
     CDS             complement(466..1020)
                     /locus_tag="VHF88_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX2160412.1"
                     /translation="MGKTLVVAEKPSVARDLATALPGSFKQGKDKAFLEGDDYVITWA
                     VGHLVGLAEPDAYDPKLKKWRFADLPIVPDEFKLIPNDERAAKQLKVIHRQMAREDVD
                     LVINACDAGREGELIFAYVYDTAKVQPPVKRLWLSSMTRKAIEDAFTRLRDGEEMKSL
                     EDAARRVHADDPVGLGARASGVLE"
     gene            1192..2754
                     /locus_tag="VHF88_01155"
     CDS             1192..2754
                     /locus_tag="VHF88_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2160413.1"
                     /translation="MASAVAASDISEAALAAEEERLARAAAAGDGDAFATLYERYERR
                     AYNLAYRVTASEDDAADAVQEAFLRVLRRLPKLEGRELVFGSYLFTATRNASYDLIGK
                     RKRAEPSDAIPDTAAPIGAAAGGGFGLDPSDPDEDPDRKLLIESQQEEIRAANERLPE
                     RQREALALRELEELSYDEIAAIMDMNRNSVAQLISRARIKLRDELRGGALASIAASSP
                     DCERALPLIAMRDDRQLDGDGDDAAWLDEHLSGCHTCRLGVEAMQEAGASYRAWAPVA
                     ASPWLFKETLAKAAELTGSDWSETISERSGRHDPAAIQPGMPSIYRAFAAPVAEARRR
                     RRRIFAAAGLGAAALLAVVATVLGQDEPEPVPAEPVTKTVETVAEPAPADVATKGPEK
                     RAKRKKPASDRAPRSGRAADTPVASSPDAPAARATPRAGAPNSSPLGRGRAPNRPGAR
                     IRQIGPQPPRTPPVELPPPAAPPAAEPVPQPPPVEEPPVEPPPVTEPPVEPPSVRNPP
                     TRPPPGSGPPIL"
     gene            complement(2832..3668)
                     /locus_tag="VHF88_01160"
     CDS             complement(2832..3668)
                     /locus_tag="VHF88_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEX2160414.1"
                     /translation="MRRSLLQIVAVAFACALALPASGAAQRAKPPNPCLDPERRAQLN
                     CPDLQMSKPFGLTLDPFVWPGHVVLRAGNSINSIGRGPAELFGIRDRRLGMRARQRIY
                     RRDGGKIGIATGARLIFKAIPGQGSYWKYLYAAKFELFRRDGRGRRGDRARRGPKVSY
                     CLRDLDRSRPSLPRSPRGFVYPGCNQSSRTRKVTLGTSVGWSDVYPPAYHEQWIDVTG
                     LRGCFDFVHTADPRNGIYESNERNNSATVTVRLPFRPGPQRCPGRQSSLPRRAPDVPL
                     DY"
     gene            complement(3709..4857)
                     /locus_tag="VHF88_01165"
     CDS             complement(3709..4857)
                     /locus_tag="VHF88_01165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX2160415.1"
                     /translation="MTPLDWIIVVFAAAMIVAGYGRGLVVGGSALVGFAVGAFAGSRL
                     GPLLLSEGQQSPWAPLMALTCALLLGAFTASVLEVLGIQLRRALRGPLGVVDGIGGAL
                     LVGALALGIAWVLGAVALQTPGARELREPIQRSLILRELNAVLPPSGPVLRALARFDP
                     LPRISGPDADVSPPDAAIARDPEVRQAGESVVRVLGTACGLGVQGTGWIARDGVVVTN
                     AHVVAGQDDTVVDVGGDGGELDAEPIWFDVRNDVALLSVPDAAGTPALSLADDPSQGA
                     SGAVLGFPENGAFDVRAARLGPTTSAISQDAYGRGPVQRSITSLRATVRSGNSGGPVV
                     DGSGRVLATVFAAATSARTPTGFGVPNDVVAEALASASGPVDTGPCVR"
     gene            4887..6578
                     /locus_tag="VHF88_01170"
     CDS             4887..6578
                     /locus_tag="VHF88_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2160416.1"
                     /translation="MTGDLTLERRRRLTHRALPAIAVLAVLAFVFGITVGGGHDSESE
                     RVARDFSAAWQREDYAAMHRLLTADARAAVSREQLAEAYDGAAATATLRRLEVGEPEV
                     DGDLARLPVTAHTAVFGEVSGPVELPVADGAIEWRPHLVFPGLGPGAALDRRTTVPER
                     AKLLAADGQVLAEGPPEARSSPLPAAASIAGSLEAPSEAAEQEALYARGFPEDTPVGV
                     SGLERVFERQLAGRPGGELLAGQRVLARSEPRPAKPVRTTIDPSVQEAADVALAGRLG
                     GVAALDPRSGEILALAGVAFSAPQPPGSTFKIVTAAAALEAGKVKPADEFPIETAATI
                     DGVELANANGESCGGDFANSFAHSCNSVFAPLGIEVGAERLVEEARQFGWNEDPGIPG
                     ALPSTIPHASGIATPLEVGSTAIGQFQTLATTLQMASVAQTIANGGIRLRPTLSPRPA
                     GAAGIRAVPPGVAKTIGRLMVGVVEFGTGTLAQVEGVEVAGKTGTAELGDTRGDDPEA
                     ALRDGDPTNTDAWFTAYAPAGKPRIAVAVLLVRNGAGGTTAAPAAQGVLVAGLKR"
     gene            complement(6583..7152)
                     /locus_tag="VHF88_01175"
     CDS             complement(6583..7152)
                     /locus_tag="VHF88_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160417.1"
                     /translation="MALRNRTLVACALSLALAGGLAACGSDHEALGVEEPAREGLAID
                     IGGLDYNVFITRELNLAITPDKDYYEGPPPEPGHNLFGVFIQVCNLGEETLPSAEEFK
                     VTDNQGVEFEPIDLPEDNAFAYNPRELAPEQCIPEDGSVAQQGPTAGSMLLFDFPLEN
                     TENRPLELEITSPATAEHESETKRFLLDL"
     gene            complement(7168..7728)
                     /locus_tag="VHF88_01180"
     CDS             complement(7168..7728)
                     /locus_tag="VHF88_01180"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2160418.1"
                     /translation="MRQVTVHTVISAPREQIFDFVADLSLRPAYCDHYLRDYRLARVD
                     PRGLGAAARFRLDGPFAKEWAEIEIKACDRPKRIVEEGRVGRLGRSRLVAVYDFIPEA
                     AGTTRVELTTFSEPKTLIDRIKQTGAAGWIRRRSSKALRRLRDIFETGQTGELPRATI
                     AGNDPGKAPRYGDHVPSQHGVSVADR"
     gene            complement(7756..7998)
                     /locus_tag="VHF88_01185"
     CDS             complement(7756..7998)
                     /locus_tag="VHF88_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160419.1"
                     /translation="MPDIGGMLLRRSVGALSSGPHCSSCRRTPLVGERLHETDAGRQL
                     CDLCLLDAPASDRKVISVRRIHASERQLPVGPRVAA"
     gene            complement(8154..>9438)
                     /locus_tag="VHF88_01190"
     CDS             complement(8154..>9438)
                     /locus_tag="VHF88_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX2160420.1"
                     /translation="GLICPAPCESACVLNINDDPVAIEEIELAIATRGFDEGWIVPCP
                     PERRTERSVGVIGSGPAGLAAAQQLNGLGHSVTVYERDEAPGGLLRFGVPDAKLEKWI
                     IDRRLKLLEEEGIVFECGVDAGRDVPVEELRRRHDALVIAIGSRVPRGLDVPGSELQG
                     VHPAMDYLYQRNRWVAREQGRPALEPAPGTEISAQGKRVVVVGGGDTGMDCVSNALRE
                     GAEDVLMLDVYPELPPSGRLEGTPWPLPPKRTLTTYALEEGGERRWASQVTEVLGRDG
                     RVAGIRARKVEGTSSRDLRAVPGSDFEQDADLVLVAIGFNHPEHEGLVEALGVDLDSR
                     GNVRAPVYGTFLEGVFACGDARIGQSLVVTAIAEGRRCARIVDRHLGGTGVALPVRAE
                     EMFAYEDDDPGSLRHQAESAGNVTVGDAFWQGPRD"
BASE COUNT         1311 a         3520 c         3392 g         1215 t
ORIGIN      
        1 gaggccgact ggatcgtcgg catgaacgcc acgcgcgccg cctcgatccg cctgcgcgca
       61 gccttcgacg gcgccgtctc gctcggccgc gtccagacgc ccacgctggc gctcgtggcg
      121 cgccgcgagg aggagatccg cgccttcaag cccgagccct actggctcgt ggaggcgcgc
      181 ttcgccgcat ccggggagcg gaagtactcc gcgcgctacc tcggcggcaa gcggatcgcc
      241 gaggacgagg cggcgaagat cgtcgaggcc tgcaaggacc gcgacggcga cgtcgacctg
      301 atcgtgaacg cctgcgacgc cggccgcgag ggcgagctga tcttcgccta cgtctacgag
      361 accgcagccg tccagaagcc cgtcaagcgg ctgtggctgt cgtcgatgac ccgcacggcg
      421 atcgaggacg ccttcgggcg gctgcgcaac ggcgaggaga tgaagtcact cgaggacgcc
      481 gctcgctcgc gctccgaggc cgactggatc gtcggcatga acgcgacggg cggcgtcctc
      541 gagcgacttc atctcctcgc cgtcgcgcag gcgcgtgaag gcgtcctcga tcgccttgcg
      601 cgtcatcgac gagagccaca gccgcttgac aggcggctgg accttcgcgg tgtcgtagac
      661 gtaggcgaag atcagctcgc cctcgcggcc ggcgtcgcag gcgttgatca cgaggtcgac
      721 gtcctcgcgg gccatttggc ggtggatcac cttgagctgc ttagcggcgc gctcgtcgtt
      781 ggggatcagc ttgaactcgt ccgggacgat cggcaggtcc gcgaagcgcc acttcttcag
      841 cttcgggtcg taggcgtcgg gctcggccag gccgacgagg tggccgaccg cccaggtgat
      901 cacgtagtcg tcgccctcga gaaaggcctt gtccttgccc tgcttgaagg atccgggcag
      961 cgctgtggcg aggtcgcgcg cgaccgaagg cttctctgcg actacgagtg tcttgcccat
     1021 gagacgcccc acgatacgca gatggccgcg ccacggcgta attccgcatg gcggcgcccg
     1081 ttggagggac agatgcagct ctcccccgcc ccctgcccct ctgccatcac gccgctgctc
     1141 ccgtgctcac ggtcctggat cgagcgcgac gggcgcgata gcttcccctc gatggcctct
     1201 gcggtggcgg cttccgacat atccgaggcg gccctggcgg ccgaggagga gcgcctggcg
     1261 cgcgccgcgg cagccggcga cggcgacgcc ttcgcgacgc tctacgagcg ctacgagcgc
     1321 cgtgcgtaca acctcgccta ccgcgtcacc gcctccgagg acgacgccgc cgacgccgtc
     1381 caggaggcct tcctgcgcgt gctgcggcgg ctgccgaagc tcgagggccg cgagctggtc
     1441 ttcggctcgt acctcttcac cgcgacccgc aacgccagct acgacctgat cggcaagcgc
     1501 aagcgggccg agccgagcga cgcgatcccc gacacggcgg cgccgatcgg cgcggcggcc
     1561 ggcggcggct tcgggctcga tccgagcgat cccgacgagg accccgatcg caagctgctg
     1621 atcgagtccc agcaggagga gatccgcgcc gccaacgagc ggctgcccga gcgccagcgc
     1681 gaggcgctcg cgctgcgcga gctcgaggag ctgtcctacg acgagatcgc cgcgatcatg
     1741 gacatgaacc gcaactcggt cgcccagctg atctcgcgcg cgcggatcaa gctgcgcgac
     1801 gagctgcgcg gcggcgcact ggcctcgatc gcggcgtcct cgcccgactg cgagcgggcg
     1861 ctgccgctga tcgcgatgcg cgacgaccgc cagctcgacg gcgacggcga cgacgccgcc
     1921 tggctcgacg agcacctgtc cggctgccac acctgccgcc tcggcgtcga ggcgatgcag
     1981 gaggccggcg cctcctaccg cgcctgggcg ccggtcgcgg cgtcgccgtg gctgttcaag
     2041 gagacgctcg cgaaggctgc cgagctgacc ggctcggact ggagcgagac gatctccgag
     2101 cggtcgggcc gccacgaccc ggcggcgatc cagcccggca tgccttcgat ctaccgggcc
     2161 ttcgcggccc cggtcgcgga ggcgcgccgg cggcgtcggc ggatcttcgc cgcggccggg
     2221 ctcggcgccg ccgccctgct cgcggtcgtc gcgaccgtcc tcgggcagga cgagcccgag
     2281 ccggtgcctg ccgagcccgt tacgaagacg gtcgagaccg tcgccgagcc ggcgccagcc
     2341 gacgtcgcga cgaaggggcc cgagaagcgg gcgaagcgga aaaagcccgc gagtgaccgc
     2401 gcgcctcggt cgggccgagc ggcggacacg ccagtcgcgt cgagccccga cgcaccagcc
     2461 gcccgcgcga caccccgggc tggcgcgccg aacagctcgc cgctcggccg cggccgagcg
     2521 ccgaaccgcc ccggagcgcg aatccgccag atcggccccc agccgccgcg cacaccgccc
     2581 gtcgagctcc cgcccccggc cgccccaccg gcggcggagc cggtccctca gccaccgccg
     2641 gtcgaggagc ctcccgtcga gccgccgccg gtgacggagc cgcccgtcga gccaccgtcc
     2701 gtccgcaacc cgccgacgcg cccgccgccc ggaagcggcc cgcccatcct ctaaccggct
     2761 cgagcgaagc cttctaaggg caacagccct aagaaagcta cgtcgggcgc ccgaaccgcc
     2821 cgaaacctcg gtcagtagtc gagcgggacg tcgggcgcgc ggcgcggtag cgacgactgc
     2881 cgccccgggc agcgctgcgg gcccggccgg aatggcagtc ggaccgtgac cgtcgcggag
     2941 ttgttgcgct cgttcgactc gtagatgccg ttgcgcggat ccgcggtgtg aacgaagtcg
     3001 aagcagccgc gcaggccggt gacgtcgatc cactgctcgt ggtaggcggg cgggtagacg
     3061 tccgaccagc cgaccgaggt ccccagcgtc accttgcgcg tcctcgacga ctggttgcag
     3121 cccggataga cgaagccccg cggcgagcgc ggcagcgacg gccggctgcg gtcgaggtcg
     3181 cgcaggcagt aggagacctt tggcccgcgg cgagcgcgat cgccgcggcg cccgcggccg
     3241 tcgcggcgga acagctcgaa cttcgccgca tagaggtact tccagtagct gccctgaccg
     3301 gggatcgcct tgaagatcag ccgggcgccg gtcgcgatgc cgatcttgcc gccgtcgcgg
     3361 cggtagatcc gctggcgcgc gcgcatgccg agacggcggt cgcggatgcc aaacagctcg
     3421 gccggcccac gaccgatgct gttgatcgag ttgcccgcgc gcagcacgac gtggccgggc
     3481 cagacgaacg ggtcgagcgt caggccgaac ggcttgctca tctggaggtc ggggcagttc
     3541 agctgcgcgc gccgctccgg atcgaggcat gggttaggcg gcttcgcgcg ctgggccgcg
     3601 cccgacgccg gcagcgcgag cgcgcaggcg aaggccacgg cgacgatttg gaggagggac
     3661 cggcgcaagg ccctctttct agcggagtcc cctgatgtgc tgctcgggtc agcgaacgca
     3721 cgggccggtg tcgacgggac cgctcgcgct cgccagcgcc tcggcgacga cgtcgttggg
     3781 cacgccgaag cccgtcggcg tgcgcgcgct ggtcgcggcc gcgaagaccg tggcgagcac
     3841 ccgaccggag ccgtcgacca cgggaccgcc ggagttgccc gaccgcaccg tcgcgcgcag
     3901 cgaggtgatc gaccgctgga cgggtcctcg gccgtaggcg tcctggctga tcgcgctcgt
     3961 cgtcgggccg agccgtgccg cgcgcacgtc gaacgcgccg ttctcgggga acccgagcac
     4021 ggcgccggac gcgccctggg agggatcgtc ggcaagggac agcgccggcg tgccggcggc
     4081 atccgggacg ctgagcagcg cgacgtcgtt gcggacgtcg aaccagatcg gctcggcgtc
     4141 gagctctccc ccgtcgccgc cgacgtcgac gaccgtgtcg tcctggccgg caacgacgtg
     4201 ggcgttggtg actaccaccc cgtcgcgcgc gatccagccg gtcccctgga cgccgagccc
     4261 gcacgcggtc cccagcacgc gcacgacgct ctcgcccgcc tggcggacct ccgggtcgcg
     4321 tgcgatcgcg gcgtcgggcg gcgacacgtc ggcgtccggg ccggagatcc gcggcagcgg
     4381 gtcgaagcgc gcgagcgctc gcaggacggg acccgagggc ggcaagacag cgttcagctc
     4441 gcgcaggatc agcgagcgct ggatcggctc gcgcagctcg cgcgcgccgg gcgtctggag
     4501 cgcgaccgcg ccgagtaccc acgcgatccc gagcgccagc gccccgacca acagcgctcc
     4561 cccgatcccg tcgaccaccc ccaggggtcc acgcagcgct cgccgcagct gaattccgag
     4621 cacctcgagc accgaggccg tgaaggcgcc gagcagcagc gcgcaggtca gcgccatcag
     4681 cggcgcccac ggcgactgct gcccctcgct cagcagcagc ggcccgagac gcgatccggc
     4741 gaacgcgccg accgcgaacc cgaccagcgc tgagccgccg acgaccagcc cgcggccgta
     4801 gccggcgacg atcatcgcgg ccgcgaagac gacgatgatc caatcgagcg gagtcatggg
     4861 ggctggacgg tagcctcccg cccaccgtga cgggcgatct gaccttggag cgccggcggc
     4921 gcctcaccca tcgtgcgctc ccggcgatcg ccgtcctcgc cgtgctggca ttcgtcttcg
     4981 gcatcaccgt cggcggcggc cacgactccg agagcgagcg cgtcgcgcgc gacttctcgg
     5041 ccgcctggca gcgcgaggac tacgcggcga tgcaccggct gctgaccgcc gacgcccgcg
     5101 ccgcggtgtc gcgcgagcag ctcgccgagg cctacgacgg cgcggcggcc accgcgacct
     5161 tgcggcggct cgaggttggc gagccggagg tcgacggcga tctagcgcga ctgcccgtca
     5221 ccgcccacac cgctgtcttc ggcgaggtca gcggaccggt cgagcttccg gtcgccgacg
     5281 gcgcgatcga gtggcggccc cacctcgtct tccccggcct cggcccggga gcagcgctcg
     5341 atcgccgcac caccgtgccg gagcgggcga agctgctggc cgccgacggc caggtgctcg
     5401 ccgagggccc gcccgaggcg cgcagctcgc cgctccccgc cgcagcctcg atcgccggct
     5461 cgctcgaggc gccgagcgag gcggcggagc aggaggccct ctacgcgcgc ggcttccccg
     5521 aggacacgcc ggtcggcgtc agcggactcg agcgcgtctt cgagcgccag ctggccgggc
     5581 gcccgggcgg cgagctgctg gccggccagc gggtcctggc gcgcagcgag ccgcggccgg
     5641 ccaagcccgt gcgcacgacg atcgacccca gcgtccagga ggccgccgac gtcgccctcg
     5701 ccggacgcct cggcggcgtc gcggcgctcg acccgcgcag cggcgagatc ctcgcgctcg
     5761 ccggcgtcgc gttctcggct ccccagccgc cgggatcgac gttcaagatc gtcaccgccg
     5821 ccgccgcgct cgaggccggc aaggtcaagc cggcggacga gttcccgatc gagacggcgg
     5881 ccacgatcga cggcgtcgag ctcgccaacg ccaacggcga gtcctgcggc ggcgacttcg
     5941 cgaacagctt cgcgcactca tgcaactccg tcttcgcccc gctcggcatc gaggtcggcg
     6001 ccgagcggct ggtcgaggag gcgcgccagt tcggctggaa cgaggacccc ggcatccccg
     6061 gcgcacttcc gagcacgatc ccgcacgcat cgggcatcgc gactccgctc gaggtcggct
     6121 cgacggcgat cggccagttc cagacgctgg cgacgacgct ccagatggca tccgtcgccc
     6181 agacgatcgc caacggcggc atccggctgc ggccgacgct ctcgccccgc cccgctggcg
     6241 ccgccggcat ccgcgccgta ccgcccggcg tcgccaagac gatcggacgc ctgatggtcg
     6301 gcgtcgtcga gttcggcacc ggcacgctcg cccaggtcga gggcgtcgag gtcgccggca
     6361 agaccggcac cgccgagctc ggcgacacgc gcggcgacga cccggaggca gccctgcgcg
     6421 acggcgatcc gaccaacacc gacgcctggt tcaccgccta cgcgccggcc ggcaagccgc
     6481 gcatcgccgt cgccgtcctg ctcgtccgca acggcgccgg cggcaccacc gccgccccgg
     6541 cggcccaggg cgtcctggtc gcgggcctga aaaggtaggc cgctagaggt ccagcaggaa
     6601 gcgcttggtc tctgactcgt gctcggccgt cgccgggctg gtgatctcca gctcgagcgg
     6661 acggttctcg gtgttctcga gcgggaagtc gaagagcagc atcgagccgg cggtcggacc
     6721 ctgctgggcg acgctgccgt cctcgggaat gcactgctcg ggggcgagct cgcgcgggtt
     6781 gtaggcgaag gcgttgtcct ccggcagatc gatcggctcg aactcgacgc cctggttgtc
     6841 ggtcaccttg aactcctcgg cgctcggcag ggtctcctcg ccgaggttgc agacctggat
     6901 gaagacgccg aacaggttgt ggccgggctc tggtggcgga ccctcgtagt agtccttgtc
     6961 cggcgtgatc gcgaggttga gctcacgcgt gatgaagacg ttgtagtcga gcccgccgat
     7021 gtcgatcgcc aggccctcgc gggccggctc ctcgaccccg agcgcctcgt gatccgagcc
     7081 gcaggcggcg agccctccgg cgagagcgag ggacagcgcg caggcgacga gggttcggtt
     7141 gcggagggcc atcggcggtc gggcagtcta cctgtcggcg acgctgacgc cgtgctgcga
     7201 tgggacgtgg tccccgtagc gcggcgcctt gcccgggtcg ttgccggcga tcgtcgcgcg
     7261 cggcagctcg cccgtctggc cggtctcgaa gatgtcgcgc agccgccgca gcgccttgct
     7321 cgagcggcgc cggatccagc ccgcggcacc ggtctgtttg atccggtcga tcagcgtctt
     7381 cggctcgctg aaggtcgtca gctcgacgcg cgtcgtgcca gccgcctcgg ggatgaagtc
     7441 gtagacggcg accagccgcg atcggccgag gcggccgacg cgcccctcct cgacgatccg
     7501 cttcggccgg tcgcaggcct tgatctcgat ctccgcccac tccttcgcga agggcccgtc
     7561 gagccgaaac cgcgccgcgg cgccgaggcc gcgtgggtcg acacgggcca agcggtagtc
     7621 acgcaggtag tggtcgcagt acgccggtcg cagcgacagg tccgcgacga agtcgaatat
     7681 ctgctcgcgc ggagcggaga tcacggtgtg aaccgttacc tgacgcacag caccggattc
     7741 tacgtgcgcg ccgcgctacg cggcgacgcg gggaccgacg ggcagctggc gctctgacgc
     7801 atggatccgc cgcacgctga tcaccttgcg atcgctcgcc ggcgcgtcga gcaggcacag
     7861 gtcgcagagc tgccgccccg catccgtctc gtgcagccgc tccccgacca gcggcgtacg
     7921 ccggcacgac gagcagtgcg ggccgctcga cagcgcaccc acgctcctcc gcaacaacat
     7981 cccgccgatg tctggcatgc agcttccttc gccgaaccac ctccgttccc tccactggag
     8041 ccgagatgta ggaaccctcc actggagccg aaggagaggg gacccgcccc cggagccgtc
     8101 ggcagaccag cgccgcgcgg gggctccggg gggcgcgagc cccccggttt ttttcagtcc
     8161 ctagggccct gccagaacgc gtcgccgacg gtgacgttgc cggcgctctc ggcctgatgg
     8221 cgcagcgagc cggggtcgtc gtcctcgtag gcgaacatct cctcggcgcg gacgggcagc
     8281 gcgacgccgg tgccgccgag gtggcggtcg acgatccgcg cgcagcggcg gccctcggcg
     8341 atcgcggtta cgaccagcga ctggccgatg cgcgcgtcgc cgcaggcgaa cacgccctcg
     8401 aggaaggtgc cgtagacggg cgcgcgcacg ttgccgcggc tgtcgaggtc gacgccgagc
     8461 gcctcgacga gcccctcgtg ctcggggtgg ttgaagccga tcgccacgag cacgaggtcg
     8521 gcgtcctgct cgaagtcgct gccagggacg gccctcaggt cgcgcgatga ggtgccctcg
     8581 accttgcgcg cgcggatgcc ggcgacgcgg ccgtcgcggc cgagcacctc ggtcacctgc
     8641 gaggcccagc ggcgctcgcc gccctcctcg agcgcgtagg tggtcagcgt ccgcttcggc
     8701 ggcagcggcc acggcgtccc ctccaggcgg ccggagggag gcagctcggg gtagacgtcg
     8761 agcatcagga cgtcctccgc gccctcgcgc agcgcgttgg agacgcagtc catgccggtg
     8821 tcgccgccgc cgacgacgac gacgcgcttg ccctgcgcgc tgatctcggt gccgggcgcc
     8881 ggctcgagcg ccggccgccc ctgctcgcgc gctacccagc ggttgcgctg gtagaggtag
     8941 tccatcgccg ggtggacgcc ctgcagctcg gagccgggca cgtcgagacc gcgcgggacg
     9001 cgcgagccga tcgcgatcac gagcgcgtcg tggcggcggc gcagctcctc gaccgggacg
     9061 tcgcggcccg cgtcgacacc gcactcgaag acgatcccct cctcctcgag cagcttcagc
     9121 cggcggtcga tgatccactt ctcgagcttc gcgtccggca cgccgaagcg cagcaggccg
     9181 ccgggtgcct cgtcgcgctc gtagacggtg acgctgtgac cgaggccgtt gagctgctgg
     9241 gcggccgcca gcccggcggg gccggagccg atcacgccga cgctgcgctc ggtgcgccgc
     9301 tcgggcgggc acggcacgat ccagccctcg tcgaagccgc gcgtcgcgat cgccagctcg
     9361 atctcctcga tcgcgaccgg gtcgtcgttg atgttcagca cgcaggccga ctcgcacggc
     9421 gccgggcaga tcaggccg
//
LOCUS       DATABV010000025         6986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2578234, whole genome shotgun sequence.
ACCESSION   DATABV010000025 DATABV010000000
VERSION     DATABV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578234"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2826
                     /locus_tag="VHF88_01195"
     CDS             <1..2826
                     /locus_tag="VHF88_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160421.1"
                     /translation="AVARAGFNGDEPTAAQRRAAIGRLAADAPERRIWDALDALDPEG
                     AGFLDSARAAAADVRPLVGAMRDRTQLPAGIQPHTGADVTVVLANNETYVSRRVARVA
                     PGVQLRVAVVNRDGFARSVEARELRDRRPVFGPIDWGEFDWGPLGSATPLAAGETELL
                     TLTPATGAFAVWLGDPAGGDQAAAMVELDRGPRTQAFRFGPDFASPRHVAEDPQGRMW
                     VTLEGIDTIARLTPAGELAQSSVERYPLPGGAHTLDSLQNPLAPADVFVDGNGIVWTT
                     LFLGNAIARIDPELARDGTGQGITIHRLDPCPTSDPATACREEFPPSPGGPSRGPLQL
                     EGTLDADGNTVLAFTEMNVDAIGLMRFSPDGRELNRADVPCDCVAPLGVALGRDGSIW
                     FSEGDTNALGRMRLDQARPYSASTARIEHHPLPFGTTIEDPEHPEPIFSALPHSVAID
                     PLGRVWLTGEAVAKVAYLDPRAARPGTSDGIVSFDTGHTEFGGLTAPADLAIDRAGTV
                     HWSDEYGDVIGRLDDGGTRAPLRPLARRSLTDSPVVDSRGDLWFMELGANLLTRVEGV
                     TEGAPRPARQPLAVASTSDDVLTGERFSEATSVDVQVVRSGAVVARAAGVPVAGGAFA
                     VGAGPAGSATARWSGPQAANPLAAGDTVRIAPRGTYEREPLTFGLAHLTAAVNTTGAL
                     AGRALADGRPLGGHVDATIGDRDLRTALNPVDGRFVQAPQPSLAPGAAGTLAWTAATV
                     GLVARTVTRFPDVPEPPAPDQPLPPLPPQPRGDVPVTPVDPGATVPAPQRLPQQSPPA
                     AQTPACADHWLTGRGRRARVELLGLPAADLARCLGAPDASASRRGVRRLRYHGKLSVE
                     VRRGRVAGFDLAPPGLRSAAGNVGLGSPVGKLRKLLRGVPLRGRGDTRRAILRTASGG
                     RAVLTVELSLRHPRRIVHVSVDRRKSR"
     gene            2823..3785
                     /locus_tag="VHF88_01200"
     CDS             2823..3785
                     /locus_tag="VHF88_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX2160422.1"
                     /translation="MSPRKVTRRTALIIALVALLVPAIAAAAWIAAPEEVELPVIPPV
                     ATVPEDAPQPQPEPAPHSGRLSAAEQRSLTAAVNDVEASASEAELAAEGRLLFRSVDV
                     AKDGESCQSCHTEGGANPALGTTPHPTFAGDFIGPRDPPSLWGVADTAPYEWDGSVAT
                     LDAMVGATIDNHFKPERSDDQGEVARQTAALVAYLRTLDPPRTAFDRGRMSEAAQRGE
                     ELFVGKAGCIACHGGPLFTDNALHDNDVPQMPGANDPGAKNPPGAFNTPQLRDLVNSA
                     PYMHNGSLRTLREVVEFYDHESSLPALNLTQDEVDDLVEYMEAL"
     gene            3934..6939
                     /locus_tag="VHF88_01205"
     CDS             3934..6939
                     /locus_tag="VHF88_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEX2160423.1"
                     /translation="MTGHQLFLNARRALVALTLLALAAPAAAQAATPSQVGAGLHIAS
                     GALVDPAGRTWVSDHNGGFCRMTEATDDAPGQIEHPQWPGDTTTTRTCLGGLLPHAAP
                     GPDAAGAPSFHDPSPEFPSSGDEIAYVPDGASPSANVLRLRWDPDTELFSFDAADPAD
                     TIVMDADVVEPDRPRPTHTALGPDGNLYVVFQRSATIQRVLAPESATPTPQVIGQTSD
                     GAGALSLTAGRDDDGAFVLYVGEPAGIRMLRPNALLPNQVTLPTPYDIGLNDPQAVLT
                     GREGAPALAFDYDDGILYAGTASELAPPGQIAGVDDVYRFDVDFPNGGSNADDPYATG
                     FSMVGGMAIGLGDELLVLDDRALLDPAEPLGEGRLFRVGPPGARITFGPSTANQTNVQ
                     SPDRAFTNNTRPTFRFAAGPARECSLQPSGQPRVWTTCTTTTTYRPPSASPLVDGESY
                     VFSVRSIDGALTGQPDSHTFTVDTTAPVTPTIARPTGAPGVVEPQRPYVELSSSETDM
                     GYACKIGGSSSFNNTIPCDTGRPFDLTGQEGSRTLRVWAVDRAGNVSSSPATRTFEVD
                     GVLPEVALDRSPNADFTQLRSISFDYEVTNEDDDVQFGCRVNAETFAICPGTLAGVRS
                     LGPNLPDGSYRFEVRARDSAGNVGEVVSRTVTVDTTKPAVDSTRLRAVTGPSGVFSLA
                     ANEPANFSCELDGAPTTCDTSLAFGSASISFNGLPDGPHTLRIDAVDRAGNASSHTHQ
                     FTVATPDTTPPTVTISAPAQGEQTQPNLYVVFAAADPTPLLTVQCRLDAVPVANCSSP
                     HLLTDMPDGFHTFTVTARDGEGNTRTLTRTWEVKRRGQGGADEAIPPVPTVTNAGPPN
                     GPQGNGPGANRVAVNGLALNPLTGDPLALGSLRVSPLVGQGAAGPTAAGVDIAVTLVA
                     DPGTRVVRLRVFQTGGAPARAAASKRKRKLVAREFRRVKAERRVRLQLSRKAVRKLRP
                     GRRYVLEVTPGTSRDTLGLPKTRRFKLSK"
BASE COUNT          947 a         2868 c         2339 g          832 t
ORIGIN      
        1 gccgtcgccc gggctgggtt caacggcgac gagccgacgg cggcgcagcg gcgcgcggcg
       61 atcggccggc tcgccgccga cgcgcccgag cgccggatct gggacgcgct cgacgcgctc
      121 gaccccgagg gggctggctt cctcgactcc gcccgcgccg ccgccgccga cgtccggccg
      181 ctggtcggcg cgatgcgcga ccgaacccag cttcccgccg gcatccagcc tcacaccggc
      241 gctgacgtca ccgtcgtgct cgccaacaac gagacctacg tctcgcgccg cgtcgcccgc
      301 gtcgcgcccg gcgtccagct gcgcgtcgcg gtcgtgaacc gcgacggctt cgcccgctcg
      361 gtcgaggcgc gcgagctgcg cgaccgccgg ccggtcttcg gcccgatcga ctggggcgag
      421 ttcgactggg ggccgctcgg ctccgcgacg ccgctcgccg ccggcgagac cgagctgctc
      481 acgctgacgc cggccaccgg cgccttcgcc gtctggctcg gcgacccagc cggcggcgac
      541 caggcggcgg cgatggtcga gctcgaccgc ggcccgcgca cgcaggcgtt ccgcttcggc
      601 cccgacttcg cctcgccgcg ccacgtcgcc gaggatccgc aaggccggat gtgggtgacg
      661 ctggagggga tcgacacgat cgctcgcctg acgcccgccg gcgagctcgc gcagtcgtcc
      721 gtcgagcgct acccgctgcc cggcggcgcg cacacgctcg actcgctcca gaacccgctc
      781 gcgcccgccg acgtcttcgt cgacggcaac ggcatcgtct ggacgacgct cttcctcggc
      841 aacgcgatcg cgcggatcga tcccgagctg gcgcgcgacg ggacggggca gggcatcacc
      901 atccatcggc tcgacccgtg cccgacctcc gatccggcaa cggcctgccg cgaggagttc
      961 ccgccgtcac ccggcggccc gagccgcggc ccgctgcagc tcgaggggac gctcgacgcc
     1021 gacggcaaca ccgtcctcgc cttcaccgag atgaacgtcg acgcgatcgg cctgatgcgc
     1081 ttctcgcccg atgggaggga gctcaaccgc gccgacgtcc cctgcgactg cgtcgctccg
     1141 ctcggcgtcg cgctcggccg cgacggatcg atctggttca gcgagggcga caccaacgcg
     1201 ctcgggcgaa tgcggctcga tcaggcgcgc ccctacagcg cgagcaccgc gcggatcgag
     1261 caccacccgc ttcccttcgg gacgacgatc gaggaccccg agcaccccga gccgatcttc
     1321 agcgcgctgc cgcacagcgt ggcgatcgac ccgctcggcc gggtctggct gaccggcgag
     1381 gcggtcgcca aggtcgccta cctcgacccg cgcgcggcgc ggccgggtac gtctgacggc
     1441 atcgtgtcct tcgacaccgg ccacaccgag ttcggcggtc tcacggcacc ggccgacctt
     1501 gcgatcgacc gcgccgggac cgtccactgg agcgacgagt acggcgacgt gatcggccgc
     1561 ctcgacgacg gcgggacgcg cgcgccgctg cgcccgctgg cccgtcgcag cctgaccgac
     1621 tcgcccgtcg tcgacagccg cggcgacctc tggttcatgg agctcggcgc gaacctgctg
     1681 acacgcgtcg agggcgtcac cgagggcgcc ccgcgccctg cccgccaacc gctggcggtc
     1741 gccagcacca gtgacgacgt gctgaccggc gagcgcttca gcgaggcgac gagcgtcgac
     1801 gtccaggtcg tgcgcagcgg cgccgtcgtg gcgcgcgccg cgggcgtgcc ggtcgccggc
     1861 ggcgcgttcg cggtcggcgc cggaccggcc ggctccgcga cggcgcgctg gagcggtccg
     1921 caggcagcga atccgcttgc cgccggcgac accgtccgca tcgccccgcg tgggacctac
     1981 gagcgcgagc cgctcacctt cgggctcgcg cacctgaccg ccgccgtcaa cacgaccggc
     2041 gcgctcgccg ggcgtgcgct cgccgacggc cggccgctcg gcggccacgt cgacgcgaca
     2101 atcggagacc gcgacctgcg cactgcgctc aacccggtcg acggccgctt cgtccaggcg
     2161 ccccagccgt cgctggcacc gggcgctgcc ggcacgctcg cctggaccgc agcgaccgtc
     2221 ggattggtcg cccgcaccgt cacccgcttc ccggacgtcc ccgagccgcc cgcgcccgac
     2281 cagccgctgc cgcccctgcc gccgcagccc cgcggcgacg ttccggtgac gccggtcgac
     2341 ccgggcgcaa ccgtgccggc accccagcgg ctaccgcagc agtcgccgcc tgccgcccag
     2401 acaccggcct gcgccgacca ctggctcacc ggccgcggtc gccgcgcgcg tgtcgagctg
     2461 ctcggcctgc ccgcggccga cctggcccgc tgcctcggcg cccccgacgc gagcgcctcg
     2521 cgccgcggcg tgcgccgcct gcgctaccac ggcaagctgt ccgtcgaggt ccgccgcgga
     2581 cgcgtcgccg ggttcgacct cgctccgccc ggtctccgct cggccgccgg caacgtcggg
     2641 ctcgggtctc cggtgggcaa gctgaggaag ctgctgcgcg gcgtcccgct gcgcggacgt
     2701 ggcgacaccc gccgcgcgat cttgcgcacg gccagcggcg gtcgcgcggt cttgacggtc
     2761 gagctgtcgc tccgccaccc gcgccgaatc gtccacgtct cggtcgaccg gaggaagtct
     2821 cgatgagccc gaggaaggtc acccggcgca ccgcgctgat catcgcgctc gtcgccctgc
     2881 tcgtgcccgc gatcgccgcg gcggcctgga tcgccgcgcc cgaggaggtc gagcttcccg
     2941 tgatcccgcc ggttgccacc gtgccggagg acgcgccgca accgcagccc gagcccgcgc
     3001 cccacagtgg gcgcctcagc gctgccgagc agcgctccct gaccgccgcc gtgaacgacg
     3061 tcgaggcgag cgcgagcgag gccgagctgg ccgccgaggg ccgcctgctc ttccgctccg
     3121 tggacgtcgc caaggacggc gagtcgtgtc agtcgtgtca caccgagggc ggcgccaacc
     3181 ctgcgctcgg cacgacgccg cacccgacct tcgccggcga cttcatcggc ccgcgcgacc
     3241 cgccgtcgct ctggggcgtc gccgacacgg cgccgtacga gtgggacggc agcgtcgcga
     3301 cgctcgacgc gatggtcggc gcgacgatcg acaaccactt caaaccggag cgctccgacg
     3361 accagggcga ggtcgcccgg cagaccgccg ccctcgtcgc ctacctgcgc acgctcgacc
     3421 cgccgcggac cgctttcgac cgcggccgga tgtccgaggc cgcccagcgc ggcgaggagc
     3481 tattcgtcgg caaggcgggc tgcatcgcct gccacggcgg cccgctcttc accgacaacg
     3541 cgctccacga caacgacgtg ccgcagatgc cgggcgccaa cgaccccgga gccaagaacc
     3601 cgcccggcgc gttcaacacg ccgcagctgc gtgacctcgt caacagcgcg ccctacatgc
     3661 acaacggctc gctgcgaacg ctgcgcgagg tggtcgagtt ctacgaccac gagtcgtcgc
     3721 ttccggcgct gaacctgacc caggacgagg tcgacgacct cgtcgagtac atggaggcgc
     3781 tgtgagcgcg cctgcgccta cgcgcagaac ggctgcgcga tcgcgcagct cccggctgtc
     3841 cgaactgctc aggcgccggc gccccgcgca gcccacgatc gattgcggat gccgcaccag
     3901 gcccgcggcc tcacgacagg agacccagaa cagatgaccg gacaccagct cttcctcaac
     3961 gcccgccgcg cgctcgtcgc gctgacgctg ctcgcgctcg ccgcgcccgc cgccgcgcag
     4021 gccgcgacgc cgtcgcaggt cggcgccggc ctgcacatcg ccagcggcgc gctcgtcgac
     4081 cccgcgggcc ggacctgggt ctccgaccac aatggcggct tctgccggat gaccgaggcg
     4141 accgacgacg cgcccggcca gatcgagcac ccgcagtggc ccggcgacac gacgacgacg
     4201 cgcacctgcc tcggcggcct gctgccgcac gccgcgcccg gccccgacgc cgccggcgcc
     4261 ccgagcttcc acgacccgtc gccggagttc ccgagcagcg gcgacgagat cgcctacgtg
     4321 ccggacggcg cgtcgccgag cgcgaacgtc ctacgcctgc gctgggaccc cgacaccgag
     4381 ctcttcagct tcgacgccgc cgacccggcc gacacgatcg tgatggacgc ggacgtcgtc
     4441 gagcccgacc gcccgcgccc gacgcacacc gcgctcggcc ccgacggcaa cctctacgtg
     4501 gtcttccagc gctcggcgac gatccagcgc gtgctcgcgc cggagtccgc gacgccgacg
     4561 ccgcaggtga tcggccagac ctccgacggc gccggcgcgc tgtcgctgac cgccggccgc
     4621 gacgacgacg gcgccttcgt cctctacgtc ggcgagccgg ccggcatccg catgctgcgc
     4681 ccgaacgcgc tgctgccgaa ccaggtcacg ctgccgacgc cgtacgacat cggcctcaac
     4741 gacccacagg cggtcctgac cgggcgcgag ggcgccccgg cgctcgcctt cgactacgac
     4801 gacggcatcc tctacgccgg cacggcctct gagctcgcgc ctcccggcca gatcgcgggc
     4861 gtcgacgacg tctaccgctt cgacgtcgac ttccccaacg gcggctcgaa tgccgacgac
     4921 ccgtacgcga ccggcttctc gatggtcggc ggcatggcga tcggcctcgg cgacgagctg
     4981 ctcgtcctcg acgaccgcgc gctgctcgac ccggccgagc cgctcggcga gggccgcctc
     5041 ttccgcgtcg gaccgcccgg cgcgcggatc accttcggcc cgagcacggc gaaccagacc
     5101 aacgtccagt cgcccgatcg cgccttcacc aacaacaccc gcccgacgtt ccgcttcgcg
     5161 gccggcccgg cgcgcgagtg ctcgctgcag cccagcggcc agccgcgggt ctggacgacc
     5221 tgcaccacga ccaccaccta ccggccgccg tcggcctccc cgctcgtcga cggcgagagc
     5281 tacgtcttct ccgtgcgcag catcgacggc gcgctgaccg gccagccgga cagccacacc
     5341 ttcaccgtcg acacgaccgc gcccgtgacg ccgacgatcg cgcgtccgac cggagcgccg
     5401 ggcgtcgtcg agccgcagcg cccctacgtc gagctctcct cgagcgagac ggacatgggc
     5461 tacgcctgca agatcggcgg cagcagctcc ttcaacaaca cgatcccgtg cgacaccggc
     5521 cggccgttcg acctgaccgg ccaggagggc agccgcacgc tgcgcgtctg ggcggtcgat
     5581 cgcgccggca acgtcagctc cagcccggcg acccggacct tcgaggtcga cggcgtcctg
     5641 cccgaggtcg cgctcgaccg cagcccgaac gccgacttca cccagctgcg ctcgatcagc
     5701 ttcgactacg aggtgaccaa cgaggacgac gacgtccagt tcggctgccg cgtcaacgcc
     5761 gagacgttcg cgatctgccc cggcacgctc gccggcgttc gcagcctcgg tccgaacctg
     5821 cccgacggca gctacaggtt cgaggtccgc gcacgcgaca gcgcgggcaa cgtcggcgag
     5881 gtcgtcagcc gcacggtcac cgtcgacacg accaagccgg ccgtcgacag cacccgcctg
     5941 cgcgcggtca cgggcccgag cggcgtgttc tcgctagccg ccaacgagcc ggcgaacttc
     6001 agctgcgagc tcgacggcgc gcccacgacc tgcgacacgt cgttggcctt cggcagcgcg
     6061 tcgatctcct tcaacggcct gcccgacggt ccgcacacgc tgcgcatcga cgccgtcgac
     6121 cgggccggca atgcgtccag ccacacgcac cagttcacgg tcgcgacgcc cgacaccacg
     6181 ccgccgactg tgacgatctc cgcgccggcc caaggcgagc agacccagcc gaacctctac
     6241 gtggtcttcg ccgcggccga cccgacgccg ctgctcacgg tccagtgccg gctcgacgcc
     6301 gtgccagtcg ccaactgctc ctcgccgcac ctgctgaccg acatgcccga cggcttccac
     6361 accttcaccg tcaccgcccg tgatggcgag ggcaacacgc gcacgctgac gcgcacgtgg
     6421 gaggtcaagc ggcgcggtca gggcggggcc gacgaggcga tcccgcccgt gccgacggtc
     6481 accaacgcgg gcccgccgaa cggccctcag ggcaacggac ccggggccaa tcgcgtcgcg
     6541 gtcaacggcc tcgcgctgaa cccgctgacc ggcgacccac tcgcgctcgg ctcgctcagg
     6601 gtgagcccgc tcgttggcca gggcgccgcc ggccccacgg cagcgggagt cgacatcgca
     6661 gtgacgctcg tcgccgatcc cggcacccgc gtcgtccgcc tgcgcgtctt ccagaccggc
     6721 ggcgcgccgg cccgcgcggc ggcatcgaag cgcaagcgca agctcgttgc gcgcgagttc
     6781 cgtcgcgtca aggccgagcg ccgcgtgcgg ctacagctga gccgcaaggc cgtgaggaag
     6841 ctgcgccccg gccgtcgtta cgtgctcgag gtcaccccgg gcaccagccg cgacacgctc
     6901 ggcctgccca agacgcgccg cttcaagctc tcgaagtagt ccctccgcca agcgcccggg
     6961 cggtcagccg actctcggcc ggccgc
//
LOCUS       DATABV010000026         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1694802, whole genome shotgun sequence.
ACCESSION   DATABV010000026 DATABV010000000
VERSION     DATABV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694802"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..663)
                     /locus_tag="VHF88_01210"
     CDS             complement(280..663)
                     /locus_tag="VHF88_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX2160424.1"
                     /translation="MDALYRDYILDHYKNPRNFGELEPHDREWHDHNPLCGDELGVHL
                     VVDDSGTITDLRFHGQGCAISQASASIAGEELIGMKADDVAALGANWVIDLLGIEISP
                     TRRKCALLSLKVMRGAVTGDASWPE"
     gene            complement(663..1871)
                     /locus_tag="VHF88_01215"
     CDS             complement(663..1871)
                     /locus_tag="VHF88_01215"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX2160425.1"
                     /translation="MRADFPILSRERDGVPIAYLDSAATSQKPTAVIEAIDSYYRHSN
                     ANVHRGVYELSQEATDLFEGARARVAAFVGGETDTTIFTRNATEAINLVAHAWGSDNV
                     GAGDEVLITHMEHHSNIVPWQLLCQRTGAKLRYLGVSDDGRLSLDELDAVLAEGRVRL
                     VSVAHVSNVLGTINPVAEIVRRASATGATTLIDGSQAVPQMPVDVAEIGADFYAWTGH
                     KALGPTGIGVLHGRRELLEAMGPFLGGGDMIAAVGFESSTWNDLPWKFEAGTSAIAEG
                     VGLGVAIDYLAALGMDAVRAHERELTAYTLERLEDVEGLRAFGPIDPDLRGGVVSLEL
                     KGIHPHDLAEICDREHVCIRAGHHCAQPLMREMGVPATARASFHVYNTRDDVDRLIAA
                     LQTARKVFAL"
     gene            complement(1916..3226)
                     /locus_tag="VHF88_01220"
     CDS             complement(1916..3226)
                     /locus_tag="VHF88_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX2160426.1"
                     /translation="MSADWLGERRKSALEAYEAEPVPTWRRSGFWTTSLRNLDLDALE
                     PRRYEPVSSPGELPVAVRDALDDEPGALIVQRGASTIHLRVDPDLAAQGVVVMPLEQA
                     VEQHGELIREWYMRRLSEGEGKFPAATAAFWTGGVFIHVPPNVRVEKPIQSVYAIDEA
                     GTAQYAHTLAIVGENAECAIREYCLGGDFDGQALHAGAFELYARPGARAKLAHFQDWG
                     RGEVHDISTKRVEIARDAFVSWVPVHLGGRLTKQTLDIVTAEQGSDMRHTGLYFTERD
                     EHLDLFTTDLHEAGHTTGDTVWKGALTGESRASYEGLIEIVENAQETHTYLQTHQMLL
                     SQRAKGDAIPSLIVKTDNVSASHGGTVGELDEDQIFYMMTRGISRIEAVRTLVEGYFE
                     EVVQRLDDPGLETLIRRRIGEKLLAAEDQVREFVGHRAGAAQEA"
     gene            complement(3216..>3615)
                     /locus_tag="VHF88_01225"
     CDS             complement(3216..>3615)
                     /locus_tag="VHF88_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2160427.1"
                     /translation="LLGENMELLRIDRAFTSRYLNEGFSGGEKKRAEILQLAMLKPEI
                     AVLDETDSGLDIDALRIVSDGVNALSGPDMGSLIITHYTRILDYVKPDFVHIMLDGRI
                     VREGGSELADELEEKGYDFIRAEVAAADVG"
BASE COUNT          503 a         1219 c         1315 g          578 t
ORIGIN      
        1 gtggcgggcg ctagcccgcc acgaccccct cttccttgcc gaccaagcgg ccgatgaaga
       61 ttctcgaaag cctcgacgac cttcccgagt tcacgagcga ggccgaggag gctgagtttt
      121 ggtcgacgca cgagctcggt ccggagattc tgaatgcgat gggcccgctt gacgacgtcc
      181 ttggccctcc ggatccaaca cggcggcccc cgattcccta ggactgggcg cgctcgcacc
      241 cggcggcgac gctgtccgcc tagctccgcc tagctccgcc tactcgggcc aggacgcgtc
      301 gccggtcacg gcgccgcgca tgaccttgag gctgaggagg gcgcatttgc ggcgggtggg
      361 ggagatctcg atgccgagca ggtcgatgac ccagttggcg ccgagggcgg cgacgtcgtc
      421 ggccttcatg ccgatcagct cctcgccggc gatcgaggcg gaggcctggg agatcgcgca
      481 gccctggccg tggaagcgca ggtcggtgat cgtgccggag tcgtcgacga cgaggtggac
      541 gccgagctcg tcgccgcaga gcgggttgtg gtcgtgccac tcgcggtcgt gcggctcgag
      601 ctcgccgaag ttgcgcgggt tcttgtagtg gtcgaggatg tagtcgcggt agagggcgtc
      661 catcagagcg cgaagacctt acgggcggtc tggagggcgg cgatcaggcg gtcgacgtcg
      721 tcgcgggtgt tgtagacgtg gaagctggcg cgcgcggtcg ctggcacgcc catctcgcgc
      781 atcagcggct gggcgcagtg gtggccggcg cggatgcaga cgtgctcgcg gtcgcagatc
      841 tcggcgaggt cgtgcgggtg gattcccttc agctcgagcg agaccacgcc gccgcgcagg
      901 tcggggtcga tcgggccgaa cgcgcgcagc ccctcgacgt cctcgagccg ctcgagcgtg
      961 taggcggtca gctcgcgctc gtgggcgcgc acggcgtcca tgccgagcgc tgccaggtag
     1021 tcgatcgcga cgccgagccc gacgccctcg gcgatcgccg aggtgcccgc ctcgaacttc
     1081 cacggcaggt cgttccaggt cgagctctcg aagccgacgg cggcgatcat gtcgccgccg
     1141 ccgaggaacg ggcccatcgc ctcgagcagc tcgcggcgac cgtgcagcac gccgatcccg
     1201 gtcggcccga gcgccttgtg gcccgtccag gcgtagaagt cggcgccgat ctcggcgacg
     1261 tcgaccggca tctggggcac ggcctgcgag ccgtcgatca gcgtcgtcgc gccggtcgcg
     1321 ctcgcccggc gcacgatctc ggcgaccggg ttgattgtcc cgagcacgtt cgagacgtgg
     1381 gctacggaga cgagcctcac gcggccctcg gccagcaccg cgtcgagctc gtcgagcgac
     1441 agccgaccgt catcggagac gccgaggtag cgcagcttcg cgccagtgcg ctggcagagc
     1501 agctgccacg gcacgatgtt cgagtggtgc tccatgtggg tgatcagcac ctcgtcgccg
     1561 gccccgacgt tgtccgaccc ccaggcgtgg gcgacgaggt tgatcgcctc ggtggcgttg
     1621 cgcgtgaaga tcgtcgtgtc ggtctcgccg ccgacgaacg cggcgacgcg cgcgcgagcg
     1681 ccctcgaaga ggtcggttgc ctcctgcgag agctcgtaga cgccgcggtg gacgttcgcg
     1741 ttcgagtggc ggtagtagga gtcgatcgcc tcgatcacgg cggtcggctt ctgcgaagtc
     1801 gccgcggagt ccagataggc gatcggcacc ccgtcgcgct cgcgcgacag gatcgggaag
     1861 tcggcgcgca cctccgcgag cggcttggtt gcggagctcg gcgcggggga cacggtcacg
     1921 cctcctgcgc cgctcccgcc cggtgtccga cgaactccct gacctggtcc tcggcggcga
     1981 gcagcttctc gccgatccgg cgccggatca gcgtctcgag gccggggtcg tcgagccgct
     2041 ggacgacctc ctcgaagtag ccctcgacca gcgtgcggac cgcctcgatg cgcgatatgc
     2101 cacgcgtcat catgtagaag atctggtcct cgtcgagctc gccgacggtg ccgccgtgcg
     2161 aggccgagac gttgtcggtc ttgacgatca gcgacgggat ggcgtcgccc ttcgcccgct
     2221 gcgagagcag catctggtgg gtctgcaggt aggtgtgcgt ctcctgcgcg ttctcgacga
     2281 tctcgatcag gccctcgtag gacgcgcgcg actcgccggt cagcgcgccc ttccagacag
     2341 tgtcgccggt cgtgtggccg gcctcgtgca ggtcggtggt gaacaggtcg aggtgctcgt
     2401 cgcgctcggt gaagtagaga ccggtgtgac gcatgtcgga gccctgctcc gcggtgacga
     2461 tgtcgagcgt ctgcttggtc agccgcccgc cgaggtggac cggcacccag ctcacgaacg
     2521 cgtcgcgcgc gatctcgacg cgcttggtcg agatgtcgtg gacctcgccg cggccccagt
     2581 cctggaagtg cgcgagcttg gcgcgcgcgc ccggccgcgc gtagagctcg aaggcgccgg
     2641 cgtgcagcgc ctggccgtcg aagtcgccgc cgaggcagta ctcacggatc gcgcactcgg
     2701 cgttctcgcc gacgatcgcc agcgtgtgcg cgtactgcgc ggtgccggcc tcgtcgatcg
     2761 cgtagacgct ctggatcggc ttctcgacgc ggacgttcgg cggcacgtgg atgaacacac
     2821 cgccagtcca gaacgccgcc gtggcggccg ggaacttgcc ctcgccctcc gagaggcgac
     2881 gcatgtacca ctcgcggatc agctcgccgt gctgctcgac cgcctgctcg agcggcatca
     2941 cgacgacgcc ctgcgcggcc aggtcgggat cgacgcgcag gtggatcgtg ctggcgccgc
     3001 gctggacgat cagcgcgcca ggctcgtcgt cgagcgcatc gcggacggcg acgggcagct
     3061 cgcccggcga gctgacgggc tcgtagcggc gcggctcgag cgcatcgagg tcgaggttgc
     3121 gcagcgacgt ggtccagaaa cccgagcggc gccaggtcgg caccggctcg gcctcatagg
     3181 cctcgagcgc agacttgcgc cgctcgccga gccagtcagc cgacatcggc cgccgccacc
     3241 tccgcccgga tgaagtcgta gcccttctcc tcgagctcgt cggcgagctc agagccgccc
     3301 tcgcggacga tccggccgtc gagcatgatg tggacgaagt ccggcttgac gtagtccagg
     3361 atgcgcgtgt agtgggtgat gatcagcgag cccatgtcag ggccgctgag cgcgttgacg
     3421 ccgtcggaga cgattcgcag ggcgtcgatg tcgaggccgg agtcggtctc gtcgagcacg
     3481 gcgatctccg gcttgagcat cgccagctgg aggatctcgg cgcgcttctt ctcgccgccg
     3541 gagaagccct cgttgaggta gcggctggtg aaggcgcggt cgatccgcag cagctccatg
     3601 ttctcgccga gcagc
//
LOCUS       DATABV010000027         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_88294, whole genome shotgun sequence.
ACCESSION   DATABV010000027 DATABV010000000
VERSION     DATABV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88294"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VHF88_01230"
     CDS             <1..528
                     /locus_tag="VHF88_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160428.1"
                     /translation="KAMKKVAGKLRIELSQYRELEAFAQFGSELDASTQATLARGERL
                     VETLNQNELRPWKPADQVAAIYSGTGGFLDRIKVERVAEFHETLLQRLHSEEAELMST
                     LNAGDWSDDIEAQLGKAIADAIDDFGPDFDVEGNPLEAGESDRILTGDGRGANGAGAE
                     ADSEPREEAEAPAAV"
     gene            533..1444
                     /gene="atpG"
                     /locus_tag="VHF88_01235"
     CDS             533..1444
                     /gene="atpG"
                     /locus_tag="VHF88_01235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX2160429.1"
                     /translation="MASQKDVKNRIGSVRNIQKITRAMEMVAAARLRRAEQRIEALRP
                     YAGAIRRMTRRTVEAVENIPNMPLLEEHERVERVGLLLVTGDRGLAGAFNSQIVRAGV
                     RRAQELSDEGTDVAWYASGRRGVGSLRFRGRDVTGAYEGFTDRPAFADARDIAGDLAT
                     AYVDGKVDRVEVIYNGYVSPLTQIVTNDVLLPLQAIDELTSGDDEGAEEAAAEERSRT
                     TSWLYEPEPEEILQRLVPDYIEISIFRALLESTASEHGARMTAMRNAQENASDMIDDL
                     TLEMNRQRQAEITQEIMEVVAGAEGLT"
     gene            1494..>1762
                     /locus_tag="VHF88_01240"
     CDS             1494..>1762
                     /locus_tag="VHF88_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160430.1"
                     /translation="MEAATATQSRAGVKPGKVEEITGVVIEAVFPEDLPEIYNALEIT
                     MPAVEDGGEPITLVCEVQQHLGDDRVRAVAMDATDGLARGTEVRDT"
BASE COUNT          314 a          619 c          620 g          209 t
ORIGIN      
        1 aaggcgatga agaaggtcgc cggcaagctg cgcatcgagc tctcgcagta ccgcgagctc
       61 gaggcgttcg cgcagttcgg ctcggagctc gacgcctcga cgcaggccac gcttgcccgc
      121 ggcgagcggc tcgtcgagac gctgaaccag aacgagctgc ggccttggaa gccggccgac
      181 caggtcgccg cgatctactc cggcaccggc ggcttcctcg accgaatcaa ggtcgagcgc
      241 gtcgccgagt tccacgagac gctgctccag cggctgcact ccgaggaggc ggagctgatg
      301 agcacgctga acgccggcga ctggtccgac gacatcgagg cgcagctcgg caaggcgatc
      361 gccgacgcga tcgacgactt cgggcccgac ttcgacgtcg agggcaaccc gctcgaggcg
      421 ggcgagtccg accgcatcct gaccggcgac ggtcgcggcg ccaacggcgc cggcgccgag
      481 gccgacagcg agccgcgcga ggaggccgag gcgccagccg ccgtctaacc ccatggcctc
      541 ccagaaggac gtcaagaacc gcatcggctc ggtgcggaac attcagaaga tcacccgcgc
      601 gatggagatg gtcgccgccg cgcgcctgcg ccgcgccgag cagcgcatcg aggcgctgcg
      661 gccctacgcg ggagcgatcc ggcggatgac gcggcggacg gtcgaggcgg tcgagaacat
      721 cccgaacatg ccgctgctcg aggagcacga gcgagtcgag cgggtcgggc tgctgctcgt
      781 caccggcgac cgcggcctcg ccggcgcctt caactcgcag atcgtgcgcg ccggcgtgcg
      841 gcgcgcgcag gagctctccg acgagggcac cgacgttgcc tggtacgcgt ctgggcgtcg
      901 cggcgtcggc tcgctgcgct tccgcggccg cgacgtcacc ggcgcctacg agggcttcac
      961 cgaccgtccc gccttcgcgg acgcgcgcga catcgccggc gacctggcca ccgcctacgt
     1021 cgacggcaag gtcgaccgcg tcgaggtcat ctacaacggc tacgtctcgc cgctgaccca
     1081 gatcgtcacc aacgacgtgc tgctgccgct gcaggcgatc gacgagctga cgtccggcga
     1141 cgacgagggc gccgaggagg ccgccgccga ggagcgcagc cgcaccacct cgtggctcta
     1201 cgagcccgag ccggaggaga ttctccagcg gctggtgccc gactacatcg agatcagcat
     1261 cttcagggcg ctgctggagt ccacggcctc cgagcacggc gcgcgcatga cggcgatgcg
     1321 caacgcgcag gagaacgcca gcgacatgat cgacgacctg accctcgaga tgaaccgcca
     1381 gcgccaggcg gagatcacgc aggagatcat ggaggtcgtc gccggggccg agggcctgac
     1441 ctagcgccga tcgacacgac ctttcgagac ttaaagagcc aaggagaacc ccagtggagg
     1501 cagcaacagc aacccagagt cgagcgggcg tcaagcccgg caaggtcgag gagatcaccg
     1561 gcgtcgtaat cgaggcggtc ttccccgagg acctgccgga gatctacaac gcgctcgaga
     1621 tcacgatgcc ggcggtcgag gacggcggcg agccgatcac cctcgtctgc gaggtccagc
     1681 agcacctcgg cgacgatcgc gtgcgcgcgg tcgcgatgga cgcgaccgac ggcctcgccc
     1741 gcgggaccga ggtgcgcgac ac
//
LOCUS       DATABV010000028         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_491183, whole genome shotgun sequence.
ACCESSION   DATABV010000028 DATABV010000000
VERSION     DATABV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491183"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VHF88_01245"
     CDS             <1..642
                     /locus_tag="VHF88_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160431.1"
                     /translation="AAAGGALSAIGGGIAGKAAAGMAAAAIVTAGAVEVKHVTKPAAP
                     VTQQAQEQPPISVHPAPPAASTAKSAPERTPVKKAAAPAKPKPAPKPEPAAAIPAPVP
                     AVEPTPATVPAPAAPAPPAVQEDEGTVTLPTAKTPAPPATTTPTAPPKPPPATTVPTP
                     APPKPPPATTTPTPPLPPPPPPVTGGAPAPAPTPPPTTGTTTAPAPPPPAPAG"
     gene            complement(722..1270)
                     /locus_tag="VHF88_01250"
     CDS             complement(722..1270)
                     /locus_tag="VHF88_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160432.1"
                     /translation="MLGISKCSAALLATVLALGLAACGDDEDDGGTTGTGAGSTTEQV
                     ETTATETTPVETPTETAGTETGGEAEGGGATPPATSPEEQEGGAGDEIPASSQASITG
                     SGGKLSPSTVRVPPFIAIRVELRSADGVQYELSGGGKTVQAGGEIETAGTTFDGLRPG
                     ERLVLSGPQGRVTVVANAEPGP"
     gene            complement(1306..1986)
                     /locus_tag="VHF88_01255"
     CDS             complement(1306..1986)
                     /locus_tag="VHF88_01255"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX2160433.1"
                     /translation="MEIRFLGQSCFELTEGDARVLIDPFLTGNPKAGAQAAELDPTHI
                     LLTHGHADHYGDTVEIAKRTGALVAAVPELANEIAGKGVENVADPNLGGTIELPGGWV
                     RWVPAWHTAVTPDGTPHVAAGLVVRLGDATVYHLGDTCLFSDLALPGRRDDLDVALMN
                     IGGHYTMDRHDAVVAAELVGARTVIPCHYDTFPPIEADAAAFKRDVEDRTSSSVVILE
                     PGETHSVG"
     gene            complement(2003..2353)
                     /locus_tag="VHF88_01260"
     CDS             complement(2003..2353)
                     /locus_tag="VHF88_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160434.1"
                     /translation="MADAERTYSDAELDAAVVALSDPERFRAAEARLARAAPQLQRVL
                     DEVLASSGGLMDTVQAQQIREAASHEDPADRMAAISGLMAEESRLGMLVGVAVGWELA
                     RELELGPAADAGND"
     gene            complement(2392..3174)
                     /locus_tag="VHF88_01265"
     CDS             complement(2392..3174)
                     /locus_tag="VHF88_01265"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2160435.1"
                     /translation="MSQPDLFVVCRNCGSEVSPYVTECPYCGQRIRKRAPKIERDEEG
                     GPQRKRRRRSRLSRIRADEIEGIAPDRRPIATMLLIALSLALTLVLASDQVGLDDVGG
                     IWLPLDDAPWRYVTAPFVHDSLAYQFVALVAVGVFGTLLERRFGVLAPLLVFLLAGAA
                     GSAAAVAVDLAPPFEDLNVGYWVLGANGAALGLLCAWLVDDRRAARRGDERGNDLIGV
                     AVFAAVLMLLPLAVAEASFVAGIVGAAAGALLGLVLPVLARR"
     gene            complement(3210..>3626)
                     /locus_tag="VHF88_01270"
     CDS             complement(3210..>3626)
                     /locus_tag="VHF88_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160436.1"
                     /translation="MDSQTRLEHTLALTALVQAMAKELGEHFEAGERLSRYPFEMLDE
                     NKWFAARHGLDGELIDLPSRDRVSTKALARRLVDRLRGHAEDLGSGDELAAIEDLIEH
                     GNGGSRQLIVYEANRDLREVVGEIVQKTSPSAPLES"
BASE COUNT          475 a         1431 c         1255 g          465 t
ORIGIN      
        1 gcagcggcag gcggagcgct ctcggcgatc ggcggcggga tcgccggcaa ggccgccgcc
       61 gggatggcgg ctgccgcgat cgtgaccgcc ggcgccgtcg aggtcaagca cgtcaccaag
      121 ccggcggctc ccgtcaccca gcaggcccag gagcagccgc cgatctccgt ccaccctgcg
      181 ccgcccgccg cctcgacggc caagtctgcg ccagagcgga cgccggtcaa gaaggccgcc
      241 gcgcctgcca agcccaagcc ggcgccgaag ccagagccgg ctgccgcgat tcccgcgccc
      301 gtcccggccg tcgagccgac gccggccacg gtcccggcac ccgctgcgcc ggccccgccg
      361 gctgtccagg aggacgaggg caccgtcacg ctcccgaccg cgaagacacc ggccccgccg
      421 gccaccacga ctccgacggc gccgccgaag ccaccgcctg cgacgaccgt cccgacaccg
      481 gctccgccga agccgccgcc cgcgacgacg acccccacac cgccgctgcc accgccaccg
      541 ccgcccgtca ccggcggcgc cccagcgccc gcgccgacgc ccccgccgac gaccggcacg
      601 actacggctc cggctccgcc tccccccgca ccggccggct agccagctcg gcacgaggcc
      661 cggtcgaacc ccgcgaacgg cggctaagcc accggcgcca ggccgcaacg ccggctgtgg
      721 cttacgggcc gggctcggcg ttggcgacga ccgtcaccct gccctgcggc ccgctcagca
      781 ccagccgctc gcctggtcgc aggccgtcga acgtcgtgcc cgcggtctcg atctcgccgc
      841 ccgcctgaac ggtcttgcca ccgccgctga gctcgtactg gacgccgtcg gccgagcgca
      901 gctcgacgcg gatcgcgatg aacggcggca cgcggacggt cgatgggctg agcttgccgc
      961 cgctgccggt gatcgatgcc tgggagctgg ccgggatctc gtcgccggcg ccgccctcct
     1021 gctcctccgg tgaggtcgca ggcggcgtcg cgccgccgcc ctcggcctct ccacctgtct
     1081 cggtgccggc ggtctcggtc ggggtctcaa cgggcgtcgt ctcggtcgcg gtcgtctcga
     1141 cctgctcggt cgtcgagccc gcgcccgtcc cggtcgtccc gccgtcgtcc tcgtcgtcgc
     1201 cgcaggccgc caggccgagc gcgaggacgg tcgccaacag ggccgctgag cacttcgaga
     1261 tccctagcat gcggcgaacc gtagccatgg ccacggaagg cctcgtcacc cgacgctgtg
     1321 cgtctcgccc ggctcgagga tcacgaccga cgagctcgtg cggtcctcca cgtcgcgctt
     1381 gaacgccgcg gcgtcggcct cgatcggcgg gaacgtgtcg tagtggcacg ggatcacggt
     1441 gcgcgctccg accagctcgg cagcgacgac cgcgtcgtgg cggtccatcg tgtagtggcc
     1501 gccgatgttc atcagcgcga catcgaggtc gtcgcgccgg cctggcagcg ccaggtcgga
     1561 gaacaggcag gtgtcgccga ggtggtagac ggtcgcgtcg ccgagccgta cgacgaggcc
     1621 ggccgccacg tgcggcgtgc cgtccggcgt caccgcggtg tgccacgccg gcacccagcg
     1681 gacccagccg ccgggcagct cgatcgtgcc gccgaggttg gggtcggcga cgttctccac
     1741 gcccttgccg gcgatctcgt tcgccagctc cggcacggcg gcgaccagcg cccccgtccg
     1801 cttcgcgatc tcaacggtgt cgccgtagtg gtcggcgtgg ccgtgggtca gcaggatgtg
     1861 cgtcggatcg agctcggcgg cctgcgcacc ggccttgggg ttgccggtca ggaacggatc
     1921 gatcaggacc cgcgcgtctc cctctgtcag ctcgaagcag gactgcccca gaaagcgaat
     1981 ctccatgccg tctcctcctc gtctagtcgt tgcctgcgtc cgccgccggg ccgagctcga
     2041 gctcgcgcgc cagctcccag ccgaccgcga cgccgaccag catcccgagg cgcgactcct
     2101 cggccatcag gccgctgatc gccgccatcc ggtcggcggg atcctcgtgg ctcgccgcct
     2161 cgcggatctg ctgggcctgg acggtgtcca tcagaccgcc gctgctggcg aggacctcgt
     2221 cgagcacccg ctgcagctgc ggcgccgcgc gcgccagccg cgcctcggcg gcgcggaagc
     2281 gctccgggtc gctgagcgcg acgaccgccg cgtccaactc ggcgtcgctg taggtgcgct
     2341 cggcgtcggc catcggcgtc cgatcatagg ccggcgacgc cggtccgcca gtcagcgccg
     2401 ggcgaggacg ggcagcacca ggccgagcag cgctccggct gccgccccga cgatcccggc
     2461 gacgaagctg gcctcggcta cggcgagcgg cagcagcatc agcaccgcgg cgaacaccgc
     2521 gacgccgatc aggtcgttgc cgcgctcgtc gccgcgccgc gccgcgcgcc ggtcgtcgac
     2581 cagccaggcg cacagcaggc cgagcgccgc gccgttggcc ccgaggaccc agtagccgac
     2641 gttgaggtcc tcgaacggcg gcgcgaggtc gaccgcgacc gccgccgccg atccggccgc
     2701 ccccgccagc aggaagacga gcagcggcgc gagcacgccg aagcgccgct cgagcagggt
     2761 gccgaagacc ccgacggcca ccagcgcgac gaactggtag gcgaggctgt catggacgaa
     2821 cggcgcggtc acgtagcgcc acggggcgtc gtcgagcggc agccagatgc cgccgacgtc
     2881 gtcgagcccg acctggtcgc tggcgagcac gagcgtcagc gcgagcgaca gcgcgatcag
     2941 cagcatcgtc gcgatcggtc gccggtcggg cgcgatcccc tcgatctcgt cggcgcgtat
     3001 ccgcgacagg cgcgaccggc ggcgccgctt gcgctgcgga ccgccctcct cgtcgcgctc
     3061 gatcttcggc gcccgcttgc ggatccgctg gccgcagtac gggcactcgg tgacgtacgg
     3121 gctgacctcc gacccgcagt tccggcacac cacgaagagg tccggctgac tcacctttct
     3181 gacgatactg cgcgcaaccg gtctggccgt cagctctcca acggcgccga cggcgacgtc
     3241 ttctggacga tctctccaac cacctctcgt aggtcacggt tggcctcgta gacgatcagc
     3301 tggcgcgagc cgccgttgcc gtgctcgatc aggtcctcga tcgccgcgag ctcgtcgccg
     3361 gagccgaggt cctcggcatg gccgcgcagg cggtcgacga gccgccgcgc gagcgccttg
     3421 gtgctgacgc ggtcgcggct cggcaggtcg atcagctcgc cgtcgaggcc gtggcgggcg
     3481 gcgaaccact tgttctcgtc gagcatctcg aacgggtagc gcgacagccg ctcacccgcc
     3541 tcgaagtgct cgccgagctc cttcgccatc gcctggacca gcgccgtcag cgcgagcgtg
     3601 tgctcgagcc gcgtctggga gtccat
//
LOCUS       DATABV010000029         6481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1776727, whole genome shotgun sequence.
ACCESSION   DATABV010000029 DATABV010000000
VERSION     DATABV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776727"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VHF88_01275"
     CDS             <1..435
                     /locus_tag="VHF88_01275"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160437.1"
                     /translation="GMTRARRELTLTHARTRALYGGREWNVPSRFLAEIPVELTDAEE
                     QVGARERATTWSSSGGGTPGIGHPGSTWESLRRDPQSPAKREPVSSGIHFSVGEDVVH
                     AKFGDGVVTDVEPGGLVVVRFAGGAGEKKLMADYAPLKRKGD"
     gene            435..1301
                     /gene="folD"
                     /locus_tag="VHF88_01280"
     CDS             435..1301
                     /gene="folD"
                     /locus_tag="VHF88_01280"
                     /inference="COORDINATES: protein motif:HMM:NF010783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX2160438.1"
                     /translation="MAAEIIDGKAVAAAVRERVAHEVEELRAVGVTPGLATILVGDDP
                     ASAVYVRMKREDCAEVGIESFHHEPGGDVSQDDLADLIAGLNADSRVHGILLQLPLPD
                     HLDQDALIPLIDRFKDVDGLTSANAGLLAQDREEALVPCTPAGVMELLAHAGVELEGA
                     RVAVIGRSILVGKPVAALLLAANATVTHCHSRTRDLAAVCRESDVIVAAVGRPSTVTA
                     DMVRDGAVVIDVGTNRVDDRLVGDVDFDAVRSKARAITPVPGGVGPMTRAMLLANTVR
                     AARAQTAAAIRQ"
     gene            1316..1882
                     /locus_tag="VHF88_01285"
     CDS             1316..1882
                     /locus_tag="VHF88_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160439.1"
                     /translation="MNLQRLRVGEWILGVSGAVLLISLFLPWWSLDGDWIDLGPGGPV
                     EGADQGPGVETTWSAWQVLSVADVLLALLGLLAIVVWTIVARASAAGPGITGEALLAP
                     FAVVMSIVVLVQVLGTPGALEAPPPFDPTVEYGAWLGLLSTLGVLAGVLVGMRDERLS
                     RPGELTDQTGAPVSAPVAVETLPGPPPA"
     gene            1923..2207
                     /gene="gatC"
                     /locus_tag="VHF88_01290"
     CDS             1923..2207
                     /gene="gatC"
                     /locus_tag="VHF88_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX2160440.1"
                     /translation="MIDREQVLHVARLARLRLDEGEVEAMTGELSSVLDHIEKIGELD
                     LDDVAPTTHVVALENVLRPDEPRPSLARDAALAEAPDSDGSGFRVPSPGA"
     gene            2204..3619
                     /gene="gatA"
                     /locus_tag="VHF88_01295"
     CDS             2204..3619
                     /gene="gatA"
                     /locus_tag="VHF88_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX2160441.1"
                     /translation="MSDVVDLTAAAAAERVRAGELSPRELWDAYRARAAADELNAFLW
                     LADEAPEVSVDAPLGGVPLAVKDLFCTAGVPSAAASRILEGYRPPYSATAVERLTGAG
                     APMLGKTNMDEFAMGSSNENSAFGPVENPWDRSKVPGGSSGGSAAAVAAGLAPWAIGT
                     DTGGSIRQPAALCGIVGLKPTYGSVSRYGMIAFASSLDQAGPLTRDVTDAALLLRHMV
                     GRDDCDSTSTGHPEQIALPSAERLDGLRFGVPPELTGDGLEPGVRAVFEATLERIEQL
                     GGTVREVGLPHSGHAISAYYVIAPAEASANLARFDGVRYGHRAAADDGLRSLYEQTRE
                     EGFGTEVKRRIMLGTYALSSGYYDAYYGRAQRVRTKIADDFRTAFESVDLIVTPTSPT
                     VAFGLGERTDDPLAMYLSDVLTVPMSLAGIPAISTPGGLSDGLPVGIQLAGPAYSENR
                     ILDAAHALELATGFEGERARA"
     gene            3612..5039
                     /gene="gatB"
                     /locus_tag="VHF88_01300"
     CDS             3612..5039
                     /gene="gatB"
                     /locus_tag="VHF88_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX2160442.1"
                     /translation="MPDVTYEPVIGLEIHVQLKTRTKMFCGCELSFGEQPNTRTCPVC
                     LGLPGTLPVVNAEAVHYGLMIGLALGCELAPRSIFHRKNYFYPDLPKGYQVSQYDIPL
                     ARDGKLGDVRIHRVHLEEDAAKLVHAGASGRIHSAEASVVDFNRGGTPLVEIVSEPDI
                     RSAAEAAEWLRLLRVTLRQLGVSDVNMEEGSLRCDANVSLRPRGEATLGVKTELKNMN
                     SFRFIERGVEAEIERQEAILRDGGRIEQETLHYDVRSGAISSLRSKEEAHDYRYFPEP
                     DLVPVVPTEQMVERARAAIPELPADRAERLGRELGLPTDQAKLLAFRTDLGDFYERAL
                     AAADGGDPRAIANWTCNELMATLGDGREPGDSKLTPEALATLVAMVVAKDVSQSAAKE
                     VLARLVAEGGDPAAIVESSGLGRADEGELAAIVDRAMADQADAVEKVRAGNDKAIGAI
                     VGAIMRETKGRADGGEVQRLIRERL"
     gene            5069..6412
                     /locus_tag="VHF88_01305"
     CDS             5069..6412
                     /locus_tag="VHF88_01305"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2160443.1"
                     /translation="MALNVVIAGGGFGGLHAARRLERRLPRHSARITLVTDQNFLLYT
                     PLLPGAASGALEPRHVVVPLREELDWADIRIARVTGADPVAKTLRMTLLDGSESEMRY
                     DQLVVSLGSVSRVLPIPGLAEHGVGFKTLPDAVALRNRAVLQLETAEAIDDPERRREY
                     LTFVYVGAGYAGLEGIAELQDYVADVIDRYPRCRLDGTRWILVEATDRVMPEIPASLA
                     EFATRELRGRGIEIRTGTLLEEVGDGWARLSTGETVPTRTVCWTAGVKPPAVVRELGL
                     PLTEHGRIDTDATLRVAGQQCVWAIGDAAAVPDPAHKGQRPSPPTAQHAVRQGKVVAD
                     NVAATLSGRSLRRFRYRTLGVFVDMGQHKAVATMLGVRLRGFPAWFAARTYHLLMMPG
                     TARRLRLMVDWTVGLMFGRASAELGALGHPEGLEAQLDQPSDPVPVPVRRSEPTE"
BASE COUNT          878 a         2351 c         2363 g          889 t
ORIGIN      
        1 gggatgacgc gggcgcggcg cgagctgacg ctgacccacg cgcggacgcg ggcgctgtac
       61 ggggggcggg agtggaacgt gccgagccgc ttcctggcgg agatcccggt cgagctgacc
      121 gacgcggagg agcaggtggg cgcacgcgag cgggcgacta cctggagctc gagcggcggt
      181 ggtacgccgg ggattgggca tcccggctcc acttgggaat cgttgcgccg ggacccccaa
      241 tccccggcta agcgggagcc ggtttcctcc ggcatccatt tcagcgtcgg cgaggacgtc
      301 gtgcacgcca agttcggcga cggggtggtc acggacgtgg agccaggcgg gctggtggtc
      361 gtgcgcttcg ccggaggcgc cggagaaaag aagctgatgg ccgactatgc gccgctcaag
      421 cgaaaggggg actgatggct gcggagatca tcgacgggaa ggcagtggcc gccgcggtgc
      481 gggagcgggt cgcccacgag gtggaggagc tgcgggcggt cggcgtcacg ccggggctcg
      541 cgacgatcct cgtcggcgac gaccccgcgt ccgcggtcta cgtgcggatg aagcgcgagg
      601 actgcgccga ggtcgggatc gagtccttcc accacgagcc cggcggcgac gtctcccagg
      661 acgacctcgc ggacctgatc gccggcctca atgccgacag ccgggtccac ggcatcctgc
      721 tccagctgcc gctgcccgac cacctcgacc aggacgcgct gatcccgctg atcgatcgct
      781 tcaaggacgt cgatgggctg acctccgcga acgccggcct gctcgcgcag gaccgcgagg
      841 aggcgctggt gccgtgcacg ccggcgggcg tgatggagct gctcgcgcat gccggcgtcg
      901 agctcgaggg cgcccgcgtc gcggtgatcg gccgctcgat cctcgtcggc aagccggtcg
      961 cggcgctgct gctggcggcc aacgccaccg tcacccactg ccactcgcgg acgcgcgatc
     1021 tcgccgcggt ctgccgcgag tccgacgtga tcgtcgcggc cgtcggccgg ccgagcaccg
     1081 tgacggcgga catggtccgc gacggagcgg tcgtgatcga cgtcggcacc aatcgcgtcg
     1141 acgaccggct cgtcggcgac gtcgacttcg atgcggtgcg gtcgaaggcg cgcgcgatca
     1201 ccccggtccc cggtggcgtc ggcccgatga cgcgcgcgat gctgctcgcc aacaccgtcc
     1261 gggcggcgcg cgcgcagacg gctgccgcta tccgccaata ggctgcggtc aggcgatgaa
     1321 cctgcagcga ctcagggtgg gggagtggat tctcggcgtg tccggcgccg tgctgctgat
     1381 ctcgctcttc ttgccgtggt ggagcctgga cggcgactgg atcgacctcg ggccgggcgg
     1441 gcccgtcgag ggggccgatc agggcccagg ggtcgagacg acatggagcg cctggcaggt
     1501 gctctcggtc gccgacgtcc tgctcgccct gctcggcctg cttgccatag tcgtctggac
     1561 gatcgtcgcg cgggcgagcg cggccggtcc gggcatcacc ggcgaggccc tgctggcccc
     1621 gttcgcggtc gtgatgtcga tcgtcgtgct cgtccaggtg ctgggcacgc cgggcgcgct
     1681 cgaggccccg ccgcccttcg acccgacggt cgagtacggc gcctggctcg ggctgctgtc
     1741 gacgctcggc gtgctcgcag gggtgctcgt cgggatgcgc gacgagcggc tcagccggcc
     1801 cggcgagctg accgaccaga ctggcgcgcc agtgtcggcg ccggtcgctg tcgagacgct
     1861 gcccggcccg ccgcccgcct gatagaggcg ccggagcggc tgcgccctag aatcgccgct
     1921 cgatgatcga ccgcgagcag gtactccacg tcgcgcgcct ggcgcgcctg cgcctcgacg
     1981 agggcgaggt cgaggcgatg acgggcgagc tctcctcggt gctcgaccac atcgagaaga
     2041 tcggcgagct cgacctcgac gatgtcgcac cgacgaccca cgtagtcgcg ctcgagaacg
     2101 tcctgcgccc cgacgagccg cgcccgagcc tggctcgtga cgccgcgctc gccgaggcgc
     2161 ccgacagcga cggcagcggc ttccgcgtcc cgagcccggg cgcatgagcg acgttgtcga
     2221 cctgacggcc gccgcggcgg ccgagcgcgt gcgtgccggc gagctgtcgc cgcgcgagct
     2281 ctgggacgcc taccgcgccc gcgctgcggc cgacgagctg aacgccttcc tgtggctcgc
     2341 cgacgaggcg ccggaggtga gcgtcgacgc cccgctcggc ggcgtcccgc tggccgtcaa
     2401 ggacctcttc tgcaccgcgg gcgtgccgag cgcggcggca tcgcggatcc tcgagggcta
     2461 ccgcccgccc tacagcgcga cggcggtcga gcggctgacc ggcgcgggcg cgccgatgct
     2521 cggcaagacg aacatggacg agttcgcgat gggttcttcg aacgagaact cggcgttcgg
     2581 gccggtcgag aacccctggg accgcagcaa ggtccccggc ggctcgtccg gcggctcggc
     2641 ggcggcggtc gccgcgggcc tcgccccgtg ggcgatcggc accgacacgg gaggatcgat
     2701 tcgccagccg gccgcgctct gcgggatcgt cgggctcaag ccgacctacg gatcggtctc
     2761 gcgctacggg atgatcgcgt tcgcgtcgtc gctcgaccag gccgggccgc tgacccgcga
     2821 cgtcaccgac gcggcgctgc tgctgcgtca catggtcggc cgcgacgact gcgactcgac
     2881 ctcgaccggg catcccgagc agatcgcgct tccgagcgcc gagcggctcg acgggctgcg
     2941 cttcggcgtt ccgccggagc tgaccggcga cggcctcgag cctggtgtgc gggcggtctt
     3001 cgaggcgacg ctcgagcgga tcgagcagct cggcggcacg gtgcgggagg tcggcttgcc
     3061 gcactccggt cacgcgatct ccgcctacta cgtcatcgcg ccggccgagg cgagcgccaa
     3121 cctcgcccgc ttcgacggcg tccgctacgg ccaccgcgcg gccgccgacg acggcctgcg
     3181 ctcgctctac gagcagaccc gcgaggaggg cttcggcacc gaggtcaagc gccgcatcat
     3241 gctcggcacc tacgcgctct cctcgggcta ctacgacgcc tactacggcc gcgcgcagcg
     3301 cgtgcgcacc aagatcgccg acgacttccg caccgccttc gagtcagtcg acctgatcgt
     3361 cacgccgacc tcgccgacgg tcgccttcgg cctcggcgag cggaccgacg acccgctggc
     3421 gatgtacctc tccgacgtgc tgacggtgcc gatgtcgctc gccggcatcc cggcgatctc
     3481 gacccccggc ggactgtccg acggcctgcc ggtcgggatc cagctcgccg gtcccgccta
     3541 cagcgagaac cggatcctcg acgcggccca cgcgctcgag ctggccaccg ggttcgaggg
     3601 ggagcgggca cgtgcctgac gtcacctacg agccggtgat cggcctcgag atccacgtcc
     3661 agctcaagac ccggacgaag atgttctgcg gctgcgagct gtcgttcggg gagcagccga
     3721 acacccgcac ctgcccggtc tgcctcgggc tgccgggcac gctgccggtc gtcaacgccg
     3781 aggcggtcca ctacgggctg atgatcggcc tggcgctcgg ctgcgagctc gcgccacgct
     3841 cgatcttcca ccgcaagaac tacttctatc ccgacctgcc gaagggctac caggtctccc
     3901 agtacgacat cccgctggcg cgtgacggca agctcggcga cgtccgcatc caccgcgtcc
     3961 acctcgagga ggacgcggcc aagctcgtcc acgccggcgc gagcggccgc atccactccg
     4021 ccgaggcgag cgtcgtcgac ttcaaccgcg gcgggacgcc gctggtcgag atcgtctccg
     4081 agcccgacat ccgctcggcc gccgaggccg ccgagtggct gcggctgctg cgcgtcacgc
     4141 tgcgccagct cggcgtctcg gacgtgaaca tggaggaggg gtcgctgcgc tgtgacgcca
     4201 acgtctcgct gcgcccgcgc ggcgaggcga cgctcggcgt caagaccgag ctcaagaaca
     4261 tgaactcgtt ccgcttcatc gagcgcgggg tggaggcaga gatcgagcgc caggaggcga
     4321 tcctgcgcga cggcggtcgg atcgagcagg agacgctcca ctacgacgtg cgcagcggcg
     4381 cgatcagctc gctgcgctcg aaggaggagg cgcacgacta ccgctacttc cccgagcccg
     4441 acctcgtccc ggtcgtgccg accgagcaga tggtggagcg cgcccgcgcg gcgatccccg
     4501 agctgccggc cgaccgcgcc gagcggctcg gccgcgagct cggcctgccc accgaccagg
     4561 cgaagctgct cgcattccgg accgacttgg gcgacttcta cgagcgggcg ttggcggcgg
     4621 cggacggcgg cgacccgcgc gcgatcgcca actggacgtg caacgagctg atggcgacgc
     4681 tcggcgacgg acgcgagccg ggcgactcga agctgacccc cgaggcgctc gcgacgctgg
     4741 tggcgatggt cgtcgccaag gacgtctcgc agtccgcggc caaggaggtg ctcgccaggc
     4801 tggtcgccga ggggggcgat cccgccgcga tcgtcgagtc gtccggtctc gggcgcgccg
     4861 acgagggcga gctggcggcg atcgtcgacc gcgcgatggc cgaccaggcc gacgccgtcg
     4921 agaaggtccg cgccggcaac gacaaggcga tcggcgcgat cgtcggcgcg atcatgcgcg
     4981 agaccaaggg ccgcgccgac ggcggcgagg tccagcggct gatccgcgag cggttgtgat
     5041 cgcggcggtc ggctagcctg cgccacggat ggccttgaac gtcgtcatcg ccggcggcgg
     5101 cttcggtggt ctgcacgccg ctcggcgact cgagcgccgc ttgccgcgcc acagcgcgcg
     5161 gatcacgctc gtaaccgacc agaacttcct gctctacacg ccgctgctgc cgggcgctgc
     5221 gtcgggtgcg ctcgagccgc gccacgtcgt cgtgccactg cgcgaggagc tcgactgggc
     5281 ggacatccgg atcgcccgcg tcaccggcgc cgatccggtc gccaagacgc tgcgcatgac
     5341 cctgctcgac ggcagcgaga gcgagatgcg ctacgaccag ctcgtcgtct cgctcggctc
     5401 ggtgtcgcgc gtgctcccga ttccggggct cgccgagcac ggcgtcggct tcaagacgct
     5461 gcccgacgcg gtcgcactgc gcaaccgcgc ggtgctccag ctcgagaccg ccgaggcgat
     5521 cgacgatccc gagcggcggc gcgagtacct gaccttcgtc tacgtcggcg ccggctacgc
     5581 cggcctagag gggatcgccg agctgcagga ctacgtcgcc gacgtgatcg accgctaccc
     5641 gcgctgccgg ctcgacggca cgcgctggat cctcgtcgag gcgaccgacc gcgtgatgcc
     5701 tgagatcccg gcgagcctgg ccgagttcgc gacgcgcgag ctgcgcgggc gcggaatcga
     5761 gatccgcacc ggcaccctgc tcgaggaggt cggggacggc tgggcgcggc tgtcgaccgg
     5821 cgagacggtg ccgacgcgga ccgtctgctg gaccgccggc gtcaagccgc ccgccgtcgt
     5881 ccgcgagctc gggctgccgc tgaccgagca cggccggatc gataccgacg cgacgttgcg
     5941 cgtcgccggt cagcagtgcg tctgggcgat cggcgatgcc gccgcggtgc ccgaccccgc
     6001 ccacaagggc caacggccga gcccgccgac ggcccagcac gcggtccgcc agggcaaggt
     6061 cgtagccgac aacgtcgccg ccacgctgtc cgggcgctcg ctgcgccgct tccgctatcg
     6121 gacgctcggc gtcttcgtcg acatgggaca gcacaaggcg gtcgcgacga tgctcggtgt
     6181 gcgcctgcgc ggcttcccgg cgtggttcgc ggcccgcacc taccatctgc tgatgatgcc
     6241 gggcaccgct cgccggctgc gcctgatggt cgactggacc gtcgggctga tgttcggacg
     6301 ggcctccgcc gagctcggtg cgctcggcca tccggagggg ctggaggcgc agctcgacca
     6361 gccgtccgat cccgttcccg tccccgttcg ccgctccgag cccaccgagt gagcaccgcg
     6421 gcgactggct acgccttccg cgaaggtcgc cagcgcgacc tgcgcacgac gttcgcgatc
     6481 t
//
LOCUS       DATABV010000030        12926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2339012, whole genome shotgun sequence.
ACCESSION   DATABV010000030 DATABV010000000
VERSION     DATABV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339012"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /gene="ffs"
                     /locus_tag="VHF88_01310"
     ncRNA           complement(<1..243)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHF88_01310"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            314..385
                     /locus_tag="VHF88_01315"
     tRNA            314..385
                     /locus_tag="VHF88_01315"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:346..348,aa:Val,seq:cac)
     gene            498..1199
                     /locus_tag="VHF88_01320"
     CDS             498..1199
                     /locus_tag="VHF88_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160444.1"
                     /translation="MVALGVDAAEVAEGIGLGGGLDALGDGADAERLGELEDRLDDGV
                     VLAAGVHAGDEGAVDLEDVDGQQVAQVAQGRVADAEVVDREADAELGELAQRLESDVG
                     VGDHCALGDLEAEPLGLDAGIRERRADDADEVVGEQLARGDVDGHREVRGARAAVLLP
                     ARNLVAGLAQHPLADRQDQAGPLGDGDEAVGRDGTERRMVPAHQGLDADHLAAAQVDG
                     GLVVEPQLTALDGVA"
     gene            1446..1772
                     /locus_tag="VHF88_01325"
     CDS             1446..1772
                     /locus_tag="VHF88_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160445.1"
                     /translation="MTLVVDVLEAEGELVAAEAGDEVGWTQHSAQPLGDGDQQLVAGL
                     VAERVVDQLEVVDVEEGDREATVAGARVAQRPAESAPEERAVRQSGERIVVRAVGQLA
                     LRLLAA"
     gene            complement(2747..3250)
                     /locus_tag="VHF88_01330"
     CDS             complement(2747..3250)
                     /locus_tag="VHF88_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX2160446.1"
                     /translation="MGQLDQAASPTAPPPKAPAFVMGLGLGGFVDGIVMHQILQWHHL
                     LTDTGDHPADTVAGLEANTLADGLFHAATWILVVVAMLLTVRAWQRGELAPPWRAHFG
                     MLLAGWGVFNLVEGLVDHQLLGIHHVRDDLGGPLGWDLAFLGLGALLILAGLALARSS
                     RAPSLSR"
     gene            3333..3719
                     /locus_tag="VHF88_01335"
     CDS             3333..3719
                     /locus_tag="VHF88_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160447.1"
                     /translation="MRNLPRLALPVCVLLALVPATLAEAKPPPTGKYDCVIGSNSILF
                     GTLAIKKGGEYAHRGTNGTFTHGKRQRQDDTGLRFYTLKFKQGDLGGMRGRWYRAKDG
                     TPSGGHEIALRNPRDDFESIYCTKRK"
     gene            3829..4944
                     /locus_tag="VHF88_01340"
     CDS             3829..4944
                     /locus_tag="VHF88_01340"
                     /inference="COORDINATES: protein motif:HMM:NF016129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HEX2160448.1"
                     /translation="MKTTLAAAIAAAALALSASTASAAVTLDWTVENTFSTGCSAPPS
                     LNCTWLGYVTNPTPFSGARGTATADRGATIAGPDGAAVIAVGGTSPRTPGTDFTFSYP
                     AAGGSLAGGASAADWAGTMQFAGRVSFVSPTPVPSAGHGFTITVDDPRVVLRGDGTGF
                     LYASGLKTQGAPGSAPVAYGDDLAVFALDLDGGTSDLDPTVAPYPPAEWRIHSDGSQT
                     LSGIVPLIENAGQAFPASPYIADSGPNRVPNIFGSFAVTMAPEIGGGRSPSDLMGPAG
                     PSGPAGARGPAGPTAPAGRNVTIVRRIQVVRLAKAPFGKPAKRVRVLRRGKLVARGRA
                     KGRTVRVRLLEGSTRRLRGRYVLRVAGGKRRAVVRLG"
     gene            complement(4952..5515)
                     /locus_tag="VHF88_01345"
     CDS             complement(4952..5515)
                     /locus_tag="VHF88_01345"
                     /inference="COORDINATES: protein motif:HMM:NF021440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HEX2160449.1"
                     /translation="MTPRVNARARTREDRAGDPLDGLVNLFDLGIVLAVAFLLAALTS
                     IDLAPSVLQQNDQERSAGAQVPADSVIAKPGEEANTIQLQPGEEVVGQGEPVGTVYRL
                     DDGRTVIVRPGSAPPVPGEQTPGVGTSGSSSSSPGSATTPDAGTGPDTPTTSPSPDST
                     PSPDGATGGAGAPSDALTAPQPSTGAE"
     gene            complement(5512..6264)
                     /locus_tag="VHF88_01350"
     CDS             complement(5512..6264)
                     /locus_tag="VHF88_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX2160450.1"
                     /translation="MTIENILFDVADALRYPVLIAAMLAVVVVVVELGALLAELRKRR
                     RRGLSRLEQGLDGIRAALAKSDNATALSLARGLGYNRPMTEALTAIVEQRGKPDAPDR
                     IAKRMAEYDYRSMRRLERTRILVRMGPALGLMGTLIPLSPALAALAEGDVQRLTEDLR
                     VAFSVTVAGLLVGMIAFAVSLVRDRLYAQDYSDVEYVLAGLSGVPIPPSPKAPPTGEA
                     KVAATEVAQSSVAPTTAVLPASPTPPTAPEGA"
     gene            complement(6331..7560)
                     /locus_tag="VHF88_01355"
     CDS             complement(6331..7560)
                     /locus_tag="VHF88_01355"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX2160451.1"
                     /translation="MASVLRPASCFALAVAALVVAVSWPALAGPPGVDVAIVKPDGTR
                     APSFIASEADHDLKGTRKVRQPDGSMTTVQIVDGISIYSLLEKAGVQSGYDAVEFVRA
                     DGTGKVRLTREQVEGVWPPGIFADDDTGAVMFIGAPPREGVDYVQARDYFELDGAALE
                     LTQLEDDVEVEVDASRTRIEAGESVEFTVEVEPEGNYRYNWTFEPGVGSKDAGKTVTH
                     RFKKAGRYKVAVGVYTAADEEHSAGDGGITIQVGDPRESDKNREGGGDNGAAAAPDSG
                     AATGSDGAAAYTPSYTPSYTPPAPSTPVTPTPPTSPPTLTPDPPKRPDIATSSGTTVE
                     GNLLADASDPPSNILESAARAARDGNPRDDEANHGGGVPEAALSVAGALALLGLGAGI
                     ETRQGRLPRLRLPRRGA"
     gene            complement(7609..8523)
                     /locus_tag="VHF88_01360"
     CDS             complement(7609..8523)
                     /locus_tag="VHF88_01360"
                     /inference="COORDINATES: protein motif:HMM:NF016129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HEX2160452.1"
                     /translation="MPLRALRLRLTPTRAALTARAGKRRIVVFSARPRKGAARIDPSA
                     ARAKLVGARLHLTGRAARLLRAKLRRSQIAAGPIGRLVLEARSPRNPARTPAPGGQGG
                     GAVPVDAPHSRPIETEPPLATRPPGALDVTTATLGWRPRESWIQYVNGGEGASVLGGA
                     ANGPQEVRPGSSAPLVYSFTGFPFRSGWYDPASGRAAVYFGGGVGFRHSAHGIDFAAV
                     EPEVELARGASRAIFRFHGSDGIRFDGRRGVLVDLHPVSIQAPAGGTVTYADVPATIP
                     ADAGQSVFAGFYAAGEPFGILTVSFTAP"
     gene            complement(8493..8804)
                     /locus_tag="VHF88_01365"
     CDS             complement(8493..8804)
                     /locus_tag="VHF88_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160453.1"
                     /translation="MLASARNALLIGMLLATVGAGPASAASGSGHVALTLGGAKPAKL
                     LATAGVDVHAISPATKRGKRLRLPVRSLAVGQAATVTLGGGIRSGRTGARCRCVHSAC
                     D"
     gene            complement(8819..9625)
                     /locus_tag="VHF88_01370"
     CDS             complement(8819..9625)
                     /locus_tag="VHF88_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2160454.1"
                     /translation="MAAPALEARGVRVAIGDKTIVHAADLTLEAGTLVAVVGPNGAGK
                     STLARAVAGMQGTAAGTVRWMGDDLARLRGRKLALRRAFIPQRARVPDGVTVAEAVRI
                     GRSPHVKPLQRPAGRDREAVAAAIERADVAEFADRKLTTLSGGELQRVQIAVALAQEA
                     PVLIADEPTSHLDLGATVTVAQLLRGLADEGLAVMLVAHDLSLAAAVADSVVVMSQGR
                     TVAAGAPLETLNPERLADVWGVDASIEAAGRHTALHLAWLGRSGAAQTRS"
     gene            complement(9625..10611)
                     /locus_tag="VHF88_01375"
     CDS             complement(9625..10611)
                     /locus_tag="VHF88_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX2160455.1"
                     /translation="MLLRRAALGAALIAAVAGGLAASLALGAVEIPLGTVIDALTGKA
                     DPGPLRQIVLDLRLPRTVEAVAVGAALGVAGALLQGALGNPLASPDVIGVTGGAGFGA
                     VLVLIAFPGSIALLPLGALAFGLLAAGLVFAIAWAGPNAGGVGRLILAGIAIAALFGA
                     GTTSVLVAYPDRVQSALFFIAGGLTSDGWSDLGVVWPYLAVGFALAISLAQPLDRLAL
                     GDDVAASLGTRPRLIRLLAGVAAALLAAAAASLAGLLGFLGLVVPHAVRLASGTSRHT
                     YVLPVSALGGSALLLAGDTLARTVKAPIELPVGPFMVAIGVPLFLFLLRRAV"
     gene            complement(10614..11471)
                     /locus_tag="VHF88_01380"
     CDS             complement(10614..11471)
                     /locus_tag="VHF88_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2160456.1"
                     /translation="MSTARILSVALVSVVALASSPAAHASPKRIVALTPFAANTLAGL
                     GVRPVAIGETLGGKERFASNLEGVARLTLSHPNGPNMEELALRNPQLVLSSPTWRKGA
                     KTMRDLEIRVVEADPANVAQAYATTKRIGELVGRAESARMLAARLRRQVHDARRGIRR
                     RPRTLVVLGVGRTPFAMLPNSWGGDIVRKAGARLVTGGVTSRSGFARISDETILAADP
                     DVIVAVPHANSDDIPAVSRFLRSNPAWKDSKAVRNGRLFVSTDNSLLQANTDVADVIR
                     RVRRSYLGN"
     gene            11588..>12926
                     /locus_tag="VHF88_01385"
     CDS             11588..>12926
                     /locus_tag="VHF88_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2160457.1"
                     /translation="MPPVDDAATATTPDAPDLARWRKAYERTPERDALFTTLSGEPIA
                     PLYTPDDLPEDAEQRIGLPGQFPYTRGVYPSMYRGRLWTMRQFAGFGTAEETNERFHY
                     LLDHGQTGLSTAFDMPSLMGHDSDAELSLGEVGREGVAIDTPEDMETLFSGIPLGEVS
                     VSMTVNAPAAIMLAFFVVAAERQGVPRERLSGTIQTDILKEYIAQKEWCFPVEPAMRI
                     VTDMVEWCTAELPRWHPISISGYHIREAGATAAQELAFTLKNGFTYVERAIERGVDVD
                     AFAPRLSFFFNAQIDFFEEIAKYRAARRIWATEMRDTYGAKDPRSLLMRFHTQTAGVS
                     LTAQQPLNNIVRTAIEALAGVLGGTQSLHTNSYDEALALPTEEAVRVALRTQQIIAGE
                     TGVTNTADPLGGSYFVEALTDRMEQQARRYFRRIDELGGMVEAVRTGFPQREIA"
BASE COUNT         1897 a         4589 c         4705 g         1735 t
ORIGIN      
        1 gttcctcggg cgcgttcgct gcactcggct ccggtcgggc caccccgcag cgcctgccgg
       61 ttaccgctta aggctgcttc cttccggacc tgacctggtt cgcgatcgct caccgcacgg
      121 gacccggcca tcgacgcctc caactggcgt actgaccccg aaggctgaac cctcgaacgg
      181 gaattcagcc ccgctagagc ggattgcggg tgcagggcac cgctacctcc ccgcctagca
      241 cggtccgccg tcgatcctag cgcggcgccc gagccggcgc gcccatcgcc acccgcctag
      301 aatcgaccgc cgagggcgcg tagctcagtg ggagagcgct cgcttcacac gcgagaggtc
      361 ggtggttcga aaccacccgc gcccatgccg tcggaagagc gcctcgtcgg gtcgagcgag
      421 ggttcggcaa ccggctcgcc cagcgtctcg cgacgaagga gctcgtcgac ggcgttcggc
      481 ttgccgggac gggcgtagtg gtagccctgg gcgtagacgc agccgaggtc gcggagggca
      541 tcggcctggg cggtggtctc gacgccctcg gcgatggcgc ggacgccgag cgccttggcg
      601 agctggagga ccgactcgac gatggcgtgg tcctcgctgc gggagttcat gccggagacg
      661 aaggagcggt cgatcttgag gacgtcgacg ggcagcaggt cgcgcaggtt gcccagggac
      721 gagtagccga tgccgaagtc gtcgatcgcg aggccgacgc cgagctcggc gagctcgcgc
      781 agcgtctcga gagcgacgtc ggggtcggcg atcactgcgc tctcggtgac ctcgaggcag
      841 agccgctggg gctcgatgcc ggcatccgtg aacgccgcgc ggacgacgcc gacgaggtcg
      901 tcggagagca gctggcgcgc ggagacgttg acggccatcg tgaggtccga ggcgcgcgag
      961 ctgccgtcct ccttccagcg cgcaacctgg ttgcaggcct cgcgcagcac ccactcgccg
     1021 atcggcagga tcaggccggt cctctcggcg atggggatga agcggtcggg cgcgacggta
     1081 ccgagcgtcg gatggtccca gcgcaccagg gcctcgacgc cgaccatctc gccgctgcgc
     1141 aggtcgacgg cgggctggta gtcgagccgc agctgaccgc gctcgacggc gtcgcgtagg
     1201 ccggtctcga gctcgaggcg ctcgacgacc cgctggcgca tcgtctcgtc gaagaactcg
     1261 gcgcgcgcct tgccgagctc cttggcgcgg tacatcgcgg catcggcgtc ggcgagcagc
     1321 gcctcggccg tctcctcgcc cctgctgagc ttgatcccga ggctcgcggt gacgaagcgc
     1381 gactctccct ggaccacgaa cggcgcccgc agcacgcttc cgagtcgctc ggcgatccgg
     1441 cgcgcatgac gctcgtcgtc gacgttctcg aagcagaagg tgaactcgtc gccgccgaag
     1501 cgggcgacga ggtcggttgg acgcagcact cggcgcaacc gctcggcgac ggcgatcaac
     1561 agctggtcgc cggcctcgtg gccgagcgag tcgttgatca gcttgaagtc gtcgatgtcg
     1621 aggaaggtga ccgcgaggcg accgtcgccg gcgcgcgcgt cgctcaacgc cctgccgagt
     1681 cggcgcccga agagcgagcg gttcggcagt ccggtgagcg gatcgtggtt cgcgcggtgg
     1741 gtcagctggc tctccgcctg cttgcggcgt gagatgtcga cgaactggca gatccaatat
     1801 ggctctgcct cgtcgacgaa cgtgtgctgc cagaggcacc atccgacggt gccgtcggcg
     1861 tgcctgaagc gatgctcctc ctcgccgtcg gcctccgtcg gacgccaggg ctctttggag
     1921 cccgagatgt cgtcgagcga catttcgagc agctgctcgc acgggcggcc ggtgaggtcg
     1981 gcgaggcggc ggttcgcgcg gcggaagcgc ccggcggcgt cgacgagcgc catcccgatc
     2041 ggggcgtcgt cgaacgccga gcggaagcgc tcctcgctgg cgagcgtgtc ccggagggcg
     2101 cgtgcgttct gggcgcgcag gtcctcgttc atgcgccagg tcgtgatgtt cgcgaggccc
     2161 agcagcgcga tgaatccgcc gtggatcccc gcccactccc acgggttccc ccaggcgccc
     2221 gagtggttgt agacggaggt cggatcgagg actccggcga gcccgtggtg gaccaccacg
     2281 taggcgacgc cgagcaggta cgggaaccac tcctcgtaga tcgagaggac ggcgaccatc
     2341 acgaagaagt agaagtgcgc ctcgatctgc ccatcccaga ggtggaccag caccgccgag
     2401 gaggtgagca gcccgatcgc aacgactccg gtgcgcacgc ggcggccgag gcgcggcgtg
     2461 cccgccgcga tcgcgcaggc ggtgatcggc gtgatctcga agagcgagtg gccgatgctg
     2521 tttccgcgcg ccagcgcgaa gaggccgagg ccgatcgcgt tgagccagac cagccagacc
     2581 atcgcgcggt ggcggcgtgc ccagtcggcc tccggcagcg acctgccgtg cggcagcagg
     2641 ccggtgaggc ggcgaagccc ggacctcgag cgtttcacgg cctcagaggg agcagcaccg
     2701 acgaccgact cgcctcccac ggcgatgctg ccactagtca tagcgcctat ctcgacagag
     2761 acggcgctcg acttgagcgc gcgagcgcga gccccgcgag gatcagcaac gcgccgaggc
     2821 cgaggaacgc cagatcccag cccagcggcc cgccgagatc gtcgcggacg tggtggatcc
     2881 cgagcagctg gtgatcgacc aggccctcga cgaggttgaa gacgccccaa ccggcgagca
     2941 gcatgccgaa gtgggcgcgc cagggcggag ccagctcgcc ccgctgccag gcacggaccg
     3001 tgagcagcat ggcgacgacg acgaggatcc aggtcgccgc gtggaagagg ccatcggcga
     3061 gcgtgttcgc ctcgaggccg gcgacggtgt cggcggggtg gtcgccggtg tcggtcagca
     3121 ggtggtgcca ctggaggatc tggtgcatga cgatcccgtc gacgaagccg ccaaggccga
     3181 ggcccatcac gaaggcgggt gccttcggcg gtggggcggt tggcgatgcg gcttggtcta
     3241 gctggcccac gtcagctcgc ctacccgcct acgaggcccg taatcgccgg agccggttgc
     3301 gcgagccgtg gagcgcgcct agggtcgccg acatgcgaaa ccttccacgc cttgccctcc
     3361 cggtctgcgt cctcctcgcc ctcgtcccgg ccacgctcgc cgaggccaag ccgcctccca
     3421 caggcaagta cgactgcgtg atcggctcga acagcatcct cttcggcacg ctggcgatca
     3481 agaagggcgg cgagtacgcc catcgcggca ccaacggcac cttcacccac ggcaagcgac
     3541 agcggcagga cgacacgggc ctgcgcttct acacgctcaa gttcaagcag ggcgacctcg
     3601 gcggaatgcg cggccgctgg taccgggcga aggacgggac gccgagcggc ggacacgaga
     3661 tcgccctgcg gaaccctcgt gacgacttcg agagcatcta ctgcacgaag cggaagtgag
     3721 ccgcgcgagg taggcgggac agtccatgtt agggtggcct aattcttagg gcgccctaac
     3781 acgatctctt cggggcgcct tcgaaaccct cgaaaggact cccgccccat gaagaccact
     3841 ctcgccgcgg cgatcgcggc agcggcgctc gcgctcagcg caagcacggc ctccgcggcc
     3901 gtgaccctcg attggacggt cgagaacacg ttctcgaccg gctgctcggc tccgccgagc
     3961 ctcaactgca cctggctcgg ctacgtcacc aacccgactc ccttctccgg cgctcgcgga
     4021 acggcgacgg ccgaccgcgg ggcgacgatc gccggacccg acggggccgc cgtgatcgcg
     4081 gtcggcggca cctcgccacg cacccctggc acggacttca cgttctccta tcccgcggcc
     4141 ggcgggtcgc tcgccggcgg ggcgagcgcc gccgactggg cgggcacgat gcagttcgcg
     4201 ggccgcgtgt cgttcgtctc cccgacgccg gtcccgtctg ccgggcacgg attcacgatc
     4261 acggtcgacg acccgcgcgt cgtcctgcgg ggcgacggca cgggattcct ctacgcgagc
     4321 ggcctgaaga cgcagggcgc gccgggcagc gctccggtgg cctacggcga cgacctcgcc
     4381 gtcttcgcgc tggacctcga tggcggcacg tccgacctcg atccgacggt ggccccgtat
     4441 ccgcccgccg agtggcggat ccactcggac ggcagccaga cgctgagcgg gatcgtgccg
     4501 ttgatcgaga acgccggtca ggccttcccc gcgagcccgt acatcgcgga cagcgggccg
     4561 aaccgcgtgc cgaacatctt cgggagcttc gcggtgacga tggcgccgga gatcggcggc
     4621 ggccgctccc cctcggacct gatgggcccc gccgggcctt ccggcccggc cggcgctcgg
     4681 gggcctgccg gtccgacggc ccccgcgggc aggaacgtca cgatcgtccg gcgcatccag
     4741 gtcgtcaggc tcgccaaggc gccgttcggc aagcccgcca agcgcgtccg cgtgctgcgg
     4801 agggggaagc tggtcgcgcg cggcagggcc aaggggagga cggtccgagt gaggctcctc
     4861 gagggctcga ccaggcgcct caggggcagg tacgtgctgc gggtcgccgg cggcaagcgc
     4921 cgggccgtcg tacggctcgg ctagcgctcg gctactcggc gccggtgctc ggctgcggcg
     4981 cggtgagcgc gtcgctggga gcgccggcgc cgccggtcgc cccatcgggc gacggagtgc
     5041 tgtccggcga cggcgaagtc gtcggtgtgt ccggcccggt gccggcgtcc ggggtcgtgg
     5101 ccgagccggg gctcgacgag ctcgagccgg aagttccgac gcccggtgtc tgctcgccgg
     5161 gaacgggcgg cgcgctgccc ggccggacga tcacggtgcg gccatcgtcg aggcggtaga
     5221 cggtcccgac cggctcgccc tgtccgacga cctcctcgcc cggctggagc tggatcgtgt
     5281 tcgcctcctc gcccggcttc gcgatcaccg agtcggcggg gacctgggcc ccggccgagc
     5341 gctcctggtc gttctgctgc agcaccgacg gcgcgagatc gatcgaggtc agcgcggcga
     5401 gcaggaacgc gaccgccagc acgatgccga ggtcgaacag gttgacgagg ccgtcgagcg
     5461 ggtcgccggc gcggtcctcg cgcgtgcgcg cgcgggcgtt gacgcgaggc gtcaagcgcc
     5521 ctccggggcc gtcggcggag tcggcgaggc gggcagcacg gcggtcgtcg gagcaacgct
     5581 cgactgagcg acctccgtcg ccgcgacctt tgcctcaccg gtcggcggcg ctttcggcga
     5641 cggcgggatc gggacgccgg acaggccggc gaggacgtac tcgacgtcgg agtagtcctg
     5701 ggcgtagagg cggtcgcgga cgagcgagac cgcgaaggcg atcatgccga cgagcagccc
     5761 cgcgacggtg acgctgaagg cgacccgcag atcctcggtg aggcgctgga cgtcgccctc
     5821 ggcaagtgcc gcgagcgccg gcgagagcgg gatcagcgtc cccatcaggc cgagcgccgg
     5881 acccatccgg acgaggatgc gcgtgcgctc gaggcggcgc atcgagcggt agtcgtactc
     5941 ggccatccgc ttcgcgattc gatccggcgc gtccggcttg ccacgctgct cgacgatcgc
     6001 tgtcagggcc tcggtcatcg gccggttgta gccgaggccg cgggcgaggc tgagcgccgt
     6061 cgcgttgtcg ctcttggcga gcgcggcgcg gatcccgtcg aggccctgct cgagccgcga
     6121 gagcccacgc cgccgccgct tgcgcagctc ggccagcaga gcgccgagct cgaccaccac
     6181 gacgacgacc gccagcatcg cggcgatcag gaccggatag cgcagcgcgt cggctacgtc
     6241 gaagaggatg ttctcgatcg tcactggttc ggcgcctcgc tctgctcgtg ggggtaagcc
     6301 gacatgtagg gggcgaatct acccaaaggg tcaagcgccg cgtcgcggca ggcgcaggcg
     6361 cggcaggcgg ccctgacggg tctcgatgcc tgcgccgagg cccagcaggg ccagcgcccc
     6421 ggcgacgctg agcgccgcct ccggcacgcc acccccgtgg ttggcctcgt cgtcgcgcgg
     6481 gttgccgtcc cgggcggcgc gggcggccga ctcgaggatg ttgctcggcg ggtcgctcgc
     6541 atcggccagc aggttgccct cgacggtcgt gcccgacgac gtcgcgatgt ccggccgctt
     6601 cggcgggtcg ggggtcagcg tcggcgggct ggtcgggggc gtgggggtca ccggggtcga
     6661 aggtgccggc ggcgtgtagg acggcgtata cgacggcgtg taggcagcag ccccgtccga
     6721 gcctgtcgcg gcgccgctgt ccggagccgc agcggcgcca ttgtcgccgc cgccctcgcg
     6781 gttcttgtcg ctctcccgcg gatcgccaac ctgaatcgtg atccccccgt cccctgcgct
     6841 gtgctcctca tcggcggcgg tgtacacccc aaccgcgacc ttgtagcgac ccgccttctt
     6901 gaagcggtgg gtgacggtct tgccggcgtc cttggaccct acgcccggct cgaaggtcca
     6961 gttgtaccgg tagttgccct ccggctcgac ctcgacggtg aactccaccg actcgccagc
     7021 ctcgatcctc gtgcgcgacg cgtcgacctc gacctcaacg tcgtcctcga gttgggtgag
     7081 ctcgagcgcc gcaccgtcga gctcgaagta gtcacgtgcc tggacatagt ccacgccctc
     7141 gcgcggcggc gcgccgatga acatgaccgc gcccgtgtcg tcatccgcga agatcccggg
     7201 gggccacacc ccctcgacct gctcgcgcgt caacctgacc ttgccggtcc cgtcggcgcg
     7261 gacgaactcg acggcgtcat agccggactg gacgccggcc ttctcgagaa gcgagtagat
     7321 cgagatgccg tccacgatct gaaccgtggt catcgagccg tccggctggc ggaccttgcg
     7381 ggttcccttg aggtcatggt ccgcctcgga ggcgatgaac gacggggctc tcgtcccgtc
     7441 gggcttgacg atcgccacgt ccacccccgg cggaccggcg agcgcgggcc agctgaccgc
     7501 gacgacgagc gccgcgacgg cgagcgcgaa acaggaggcc ggtcgaagga cagacgccat
     7561 ccggggcatg ttaggcaagc tccacgagca cgcgtcgcgc ttccgccatc agggagcggt
     7621 gaaggagacg gtgaggatcc cgaagggctc gccggccgcg tagaagccgg cgaagacgga
     7681 ctggccggcg tcggccggga tcgttgccgg gacgtccgcg taggtgacgg tcccgccggc
     7741 cggcgcttgg atcgacacgg gatgcaggtc gactaggacg ccgcgccgac cgtcgaagcg
     7801 gatcccgtcg ctgccgtgga agcggaagat cgcccgcgac gcgccgcggg ccagctcgac
     7861 ctccggctcg acggcggcga agtcgatgcc atgcgcgctg tgccggaagc ccacgccgcc
     7921 gccgaagtag accgccgccc taccgctggc cgggtcgtac cagccgctgc ggaacgggaa
     7981 gcccgtgaag gagtagacga gcggcgccga ggagccgggg cgcacctcct gcggtccgtt
     8041 ggccgccccg ccgaggaccg acgcgccctc gccaccgttt acgtactgga tccaggactc
     8101 gcgcggacgc cagccgagcg tcgcggtcgt gacgtcgagc gcgccaggag gacgcgtcgc
     8161 cagtggcggc tcggtctcga tcggcctgga gtgcggcgcg tcgaccggga cggccccgcc
     8221 gccctgaccg ccgggggcgg gcgtgcgggc ggggtttcgt ggcgatcgcg cttcgaggac
     8281 gagcctgcca atcggtccgg cggcgatctg cgaccggcgg agcttggcgc gtaggaggcg
     8341 ggcggcgcgc cccgtcagat gcagcctggc gccgaccagc ttcgccctcg ccgccgacgg
     8401 atcgattctg gccgcgccct tgcgcggcct tgccgagaag acgacgatgc gtctcttccc
     8461 ggctcgagcg gtgagcgcgg cccgtgtggg agtcagtcgc aggcggagtg cacgcagcgg
     8521 cagcgagcgc cggtccgccc cgagcggatg ccgccgccca gcgtgacggt cgccgcctgc
     8581 ccgacggcga ggctgcggac cgggagccgg agccgcttcc cgcgcttggt ggctggagag
     8641 atcgcgtgga cgtcgacgcc ggcggtggcg agcaacttgg ccggcttggc cccgccgagc
     8701 gtcagcgcga cgtggccgct gccgctggcg gccgaggcag gaccggcgcc gacggtggcg
     8761 agcagcatcc cgatgagcag cgcgttacga gctgaggcaa gcatcgagtt agggtaccct
     8821 aacttctagt ttgagcagcg cctgaccgtc caagccaggc gagatgaagc gcggtatggc
     8881 ggcccgccgc ctcgatcgac gcgtccacgc cccagacgtc agccaggcgt tcggggttga
     8941 gcgtctcgag cggcgccccc gccgcgacgg tccgcccctg cgacatcacg accacgctgt
     9001 cggctacggc ggcggcaagc gagaggtcgt gcgccacgag catcacggca aggccctcgt
     9061 cggcgagtcc gcgcagcagc tgagcgacgg tgaccgtcgc cccgagatcc aggtgcgagg
     9121 tcggctcgtc ggcgatcagc accggagcct cctgggcgag cgcgacggcg atctggacac
     9181 gctgcagctc gccgccggag agggtcgtga gcttgcgatc ggcgaactcg gcgacatctg
     9241 cgcgctcgat cgcggcggcg accgcctcgc ggtcgcgacc cgccggtcgc tgcagcggct
     9301 tgacgtgcgg ggagcggccg atccgcactg cctcggcgac cgtcactccg tcgggcacgc
     9361 gcgcccgctg ggggatgaag gcgcgccgca gcgcgagctt gcggccgcgc aggcgagcga
     9421 ggtcgtcgcc catccagcgg accgtgccgg cggccgtccc ctgcatgccc gcgacggcgc
     9481 gcgcgagggt cgacttgccg gcgccgttgg gcccgacaac cgcgacgagc gtgcccgcct
     9541 cgagagtcag gtcggccgcg tggacgatcg tcttgtcgcc gatcgcgacc cggacgccgc
     9601 gggcctcgag cgccggcgcc gccatcagac ggccctccgc agcaggaaca ggaacagcgg
     9661 cacgccgatc gcgaccatga acggccccac cggcagctcg atcggcgcct tgaccgtgcg
     9721 cgcgagcgtg tcgccggcga gcagcagcgc gctgccgccg agcgcggaga ccggcagcac
     9781 gtaggtgtgg cgcgacgtgc cggaggcgag ccgcacggcg tgcgggacga cgaggccgag
     9841 gaacccgagc aggccggcta gcgaggcggc cgccgccgcg agcagcgcgg cggcgacgcc
     9901 cgccagcagc cggatcagcc gcggacgcgt cccgagcgag gccgcgacgt cgtcgccgag
     9961 cgcgagccgg tcgagcggct gcgccaggct gatcgccagc gcgaagccga cggcgaggta
    10021 aggccagacg accccgaggt ccgaccagcc gtcggaggtc aatccgcccg cgatgaagaa
    10081 cagcgccgac tggacgcggt cgggataggc gacgagcacc gacgtcgtgc cggcgccgaa
    10141 cagcgcggcg atcgcgatcc cggcgaggat cagccggccg acgccgccgg cgttcggccc
    10201 cgcccaggcg atcgcgaaga cgaggcccgc cgcgagcagg ccaaaggcca gcgcgccgag
    10261 cggcagcagc gcgatcgagc cggggaaggc gatgaggacc agcaccgcgc cgaagccggc
    10321 gccgccggtg actccgatca cgtcgggcga ggcgagcggg ttgccgagcg ctccctgcag
    10381 cagcgcgccg gcgacgccga gcgccgcgcc gacggcgacc gcctcgaccg tccgcggcag
    10441 gcgcaggtcg agcacgatct ggcgcagcgg gcccgggtcc gccttgccgg tcagcgcgtc
    10501 gatcaccgtg ccgagcggga tctcgaccgc gccgagcgcc agcgacgccg cgaggccgcc
    10561 ggcgacggcc gcgatcaacg cggcgccgag cgccgctcgg cggagcagca cgctcagttg
    10621 cccaggtacg agcgccgcac ccggcggatc acgtcggcga cgtcggtgtt cgcctgcagc
    10681 agcgagttgt cggtcgacac gaacaggcgg ccgttgcgaa ccgccttgga gtccttccat
    10741 gccgggttcg agcgcaggaa cctcgagacc gccgggatgt cgtcggagtt cgcgtgcgga
    10801 acggcgacga tcacgtcggg gtctgcggcg aggatcgtct cgtcggagat ccgcgcgaag
    10861 ccactcctgc tcgtcacccc accggtgacc agcctcgcgc ccgccttgcg gacgatgtcc
    10921 ccgccccagc tgtttggcag catcgcgaac ggcgtccggc cgacgccgag cacgaccagc
    10981 gtgcgcgggc gcctgcggat gcctcgccgc gcgtcgtgca cctgccgtcg caggcgcgcc
    11041 gcgagcatgc gcgcactctc ggcacggccg acgagctcgc cgatccgctt cgtcgtcgcg
    11101 taggcctgcg cgacgttcgc ggggtcggcc tcgacgacgc ggatctcgag gtcgcgcatc
    11161 gtcttggcgc ccttcctcca ggtcggtgac gagagcacca gctgcgggtt gcgaagcgcg
    11221 agctcctcca tgttcggccc gttcggatgc gagagcgtga gccgcgcgac gccttcgagg
    11281 ttcgaggcga agcgctcctt cccgccgagc gtctcgccga tcgcgaccgg cctgacgccg
    11341 agaccggcga gcgtgttggc ggcgaatggg gtcagcgcga cgatccgctt gggactcgcg
    11401 tgcgctgcgg ggctcgaggc gagcgccacg acggatacga gggcgacgga gaggatcctt
    11461 gctgtgctca tatcgagtta gggtacccaa acttaggctc ccctaagtca ttgcttggtc
    11521 aaatcccacc gcgccagata cgaggaagtc ctactatcta ggttccacct acctcgagga
    11581 gcagttgatg ccgccggtcg acgacgccgc cacagcgacc accccagacg cgcctgacct
    11641 cgcccgctgg cgcaaggcct acgagcgcac gcccgagcgc gacgcgctct tcacgacgct
    11701 ctccggcgag ccgatcgcgc cgctctacac gcccgacgac ctgcccgagg atgccgagca
    11761 gcggatcggg ctgccgggcc agttccccta cacgcgcggc gtctacccgt cgatgtaccg
    11821 cggccggctc tggacgatgc gccagttcgc cggcttcggc acggccgagg agaccaacga
    11881 gcgcttccac tacctgctcg atcacggcca gaccggcctc tcgacggcct tcgacatgcc
    11941 gtcgctgatg ggccacgact ccgatgccga gctgagcctc ggcgaggtcg ggcgcgaggg
    12001 cgtcgcgatc gacaccccgg aggacatgga gacgctcttc tccggcatcc cgctcggcga
    12061 ggtctcggtc tcgatgaccg tcaacgcgcc ggcggcgatc atgctcgcct tcttcgtcgt
    12121 cgcggccgag cgccagggcg tgccgcgcga gcggctgtcc gggacgatcc agaccgacat
    12181 cctcaaggag tacatcgccc agaaggagtg gtgcttcccg gtcgagccgg cgatgcggat
    12241 cgtcaccgac atggtcgagt ggtgcaccgc cgagctgccg cgctggcacc cgatctcgat
    12301 ctccggctac cacatccgcg aggccggagc gacagcggcg caggagctcg cgttcacgct
    12361 caagaacggc ttcacctacg tcgagcgggc gatcgagcgc ggcgtcgacg tcgacgcgtt
    12421 cgcgccgcgg ctgagcttct tcttcaacgc ccagatcgac ttcttcgagg agatcgcgaa
    12481 gtaccgcgcg gcgcgccgaa tctgggcgac cgagatgcgc gacacctacg gcgcgaagga
    12541 tccgcgctcc ctgcttatgc gcttccacac ccagaccgcg ggcgtctcgc tgaccgcgca
    12601 gcagccgctc aacaacatcg tgcgcacggc gatcgaggcg ctcgccggcg tgctcggcgg
    12661 cacccagtcg ctgcacacca actcctacga cgaggcgctc gcgctgccga ccgaggaggc
    12721 ggtgcgcgtc gcgctgcgca cccagcagat catcgccggc gagacgggcg tcacgaacac
    12781 cgccgacccg ctcggcggct cctacttcgt cgaggcgctg accgaccgga tggagcagca
    12841 ggcgcgacgt tacttccgcc gcatcgacga gctcggcggg atggtcgagg ccgtccgcac
    12901 cggctttccg cagcgtgaga tcgccg
//
LOCUS       DATABV010000031        21530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1777045, whole genome shotgun sequence.
ACCESSION   DATABV010000031 DATABV010000000
VERSION     DATABV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21530
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777045"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VHF88_01390"
     CDS             <1..830
                     /locus_tag="VHF88_01390"
                     /inference="COORDINATES: protein motif:HMM:NF021883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX2160458.1"
                     /translation="TILLDYAQHLAIRVVDGMTVHEDRMRENLNLTHGALYSQRALLA
                     LVESGRSRDDAYRIVQENAQRAWDEGIEFRNLLAGAGLERFATELRHLQRTEVLEARE
                     PFRAGQKGSSAMPHKRNPIVSERITGIARLLRGYAQAGLEDVALWHERDISHSSVERV
                     ALPDATILLDYAHHLATRVVEGMVVNADRMRANIDLTHGALFSQRALLALVESGRSRD
                     DAYRIVQENAQHAWDEGIEFRDLLAEAAPELDLDAIFDPTAFTRHAADIVARLDSLD"
     gene            866..1762
                     /locus_tag="VHF88_01395"
     CDS             866..1762
                     /locus_tag="VHF88_01395"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2160459.1"
                     /translation="MTEAAALADLQPVAQGKVRDIYAVGEDRLLLVTSDRISTYDAVH
                     PTPIPDKGKVLTGLSCFWFDRTEQIVPNHLVSYTEVPDEVRGRGLLVERLEMAQVECV
                     VRGYITGSGWKDYQASGAVCGIELPSGLQESEQLPEPIFTPATKAAVGDHDENVDFDR
                     AAEILGDRPLLEELRRLSLALYEFGAAHARERGIILADTKFEFGRRPDGTIVLADEVL
                     TPDSSRFWPADGYEPGRGQPSFDKQFVRDWASGSGWDKSPPAPAIPDDVIAGTRERYV
                     EAYERIADEPFSAWLERSRSAG"
     gene            1759..1983
                     /gene="purS"
                     /locus_tag="VHF88_01400"
     CDS             1759..1983
                     /gene="purS"
                     /locus_tag="VHF88_01400"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX2160460.1"
                     /translation="MRARVLIRPKEGILDPQGQAVERALPALGFEGVANVHVGRLVEL
                     DVDSVDAVPEMCERLLANPLIEDYEVLAVE"
     gene            1980..2645
                     /gene="purQ"
                     /locus_tag="VHF88_01405"
     CDS             1980..2645
                     /gene="purQ"
                     /locus_tag="VHF88_01405"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX2160461.1"
                     /translation="MKFGVVRFPGSCDEVDALLACEQQGEAEILWHGDRDLKGVDAVV
                     IPGGFSYGDYLRVGAIARFSPVMEAVAEFARDGGPVLGICNGFQVLCEAGLLPGALLP
                     NTELRFLCRQVDVRVVNPDTPFTRACDVDQTLSIPVKHTTGRYYAPDATIAEMEDVGQ
                     VVFRYAESWNPNGSVRDIAGVCNRERNVVGLMPHPEHAVDPLTGSADGACLFASAAQM
                     VAA"
     gene            2642..4873
                     /gene="purL"
                     /locus_tag="VHF88_01410"
     CDS             2642..4873
                     /gene="purL"
                     /locus_tag="VHF88_01410"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX2160462.1"
                     /translation="MSTEASAPDLRAEARALGLTDSEYDLIGAKLGRPPNGLELAVFS
                     LMWSEHCAYKHSKKLLGRLPVEGPHLLMGPGENAGAVDVGDGLAIAFKVESHNHPSAV
                     EPFQGAATGVGGIMRDIFAVGARPIAVLDSLRFGELDSPRSRYLLDGAVSGIGHYGNS
                     IGVATVGGEVYFEGPYEQNCLVNAMCLGIAPQERLTRSAAAGVGNHLVLMGARTGRDG
                     IGGASVLASAELDSEDADKRPTVQIGDPFEEKKVMEACLELLDRELLASLQDLGAAGL
                     SSSSSEMASKGEVGLDIDVSRVPLREADMEPFEIMVSESQERMLAVVEPEQLAAVEAV
                     CERWEVRCTVIGSVTDSRHLRVFDGDELVGDMPVDALVDDCPLYDLAPEQPVEQIYPA
                     PRRTLAPSDDLAENLLALLGSSNVASRRPLFEQYDSLVQSRTVRRPEAADAAVLQLKH
                     DTGGGSSAIAVSIDGNGRRVACDPYWGTVEAVLECASNLACVGAEPLGLTNCLNFGNP
                     EKPHIAWQLSRSVDGMADALHQLGVPVVGGNVSLYNQSGDGPIYPTPVVGMVGLVPDA
                     ARAAAVGLGFREEGHTIAMVGTPQPSLAGSELAKLRGEPLPTSLPEIDLAAAKRAQEA
                     VRDAVRAGGLASVHDIAEGGFLVAIAECCLAGDIGARLDFGSADVDWAALEPYLFGEG
                     VGGFVVSGEREAIDRLAERVPTDVFGKVGGDALWVGLGGSAPRVPLARLHAAHGALAA
                     AFP"
     gene            complement(4895..5482)
                     /locus_tag="VHF88_01415"
     CDS             complement(4895..5482)
                     /locus_tag="VHF88_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2160463.1"
                     /translation="MPRRAKVRPEEAGGERVLAAGRELFAERGYHATSIADIGERAGI
                     AKSVLYHHFGSKAQLYEAIVEAETRDLIERVRDAVPTRPGASRLRPGVEAYLGFLAER
                     PAAWRLLLRDPPAEPALIAVHERLSEQRAAALAELLATPGKRLRQDPHLDLTATAIRA
                     FAAWWYQHRDVPREQVVEAILDVARAGARRLTAAA"
     gene            5564..6448
                     /locus_tag="VHF88_01420"
     CDS             5564..6448
                     /locus_tag="VHF88_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2160464.1"
                     /translation="MASARTILISGASGAIGADVVRSLARRGARLAISARRADILMAL
                     ADEIEAAGGIRPLVLSADLSQRGEAGELARRATDALGDVDVLVNNAGASVQGLIATVA
                     DRDEARHVFETNLWSPLALVAELAPRMLERGAGTIVNVGSMVRVSPYPHLGHYAASRA
                     ALALATEVLELELAPRGVRVVEVALGPIDTPASRENRLLAGADRWLDGRPGVGRADAA
                     ARAIVAAVEGDARGVVFHPRSLRWADRLPALGRRYSRRMARGADLFDHTVRLGGSAGD
                     PELQAVHRDWAATAGDPR"
     gene            6509..7972
                     /locus_tag="VHF88_01425"
     CDS             6509..7972
                     /locus_tag="VHF88_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160465.1"
                     /translation="MGMQGAQAELRGRWRRWLASPVPLTLLAAAVLPASASAAPDFKV
                     ALPSAGDMTVAVVNFKVDPPKDKRKLGKLVGDLTLETTAKKKKKLDAAVLSSLVGRRV
                     GDKLKVTGLVTLVRPEDAKEASSEKLKKLGFELTDGMPSSAEKLAKLLAQERKRKEQL
                     AKQLQVLKLEHQKLAKQLVQPGATGFEDVNLRLLEVLQLIREVTETRREVERRVRELD
                     TELRKQQLLEAAKQLKRSEGGLGDPKVVGDVKKSESKNALENPKKTGEVLAKTKLAGK
                     LIKKLDDEHSKQRVDEKELGKAIIQAFLDKKDTEKQDQALEKLKRSLESQKCKKGALI
                     LLCEDQKPPTLETPNAPPSAPPKLTPTPGPNDPLPPPDEPAPGPELAISLSHSDEGWF
                     NFCIDIATTPPQPGASGTYSFGESGTEGFGPEPFVLDENGRAKVVFEGPDPAVVDSFF
                     AEVTLDWPGIGPITERIEGPGWGSLVGTDECSIDLEG"
     gene            8085..9524
                     /gene="purF"
                     /locus_tag="VHF88_01430"
     CDS             8085..9524
                     /gene="purF"
                     /locus_tag="VHF88_01430"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX2160466.1"
                     /translation="MTSHPISDRDGPRDECGVFGVLAPESEVARLAYFGLFALQHRGQ
                     ESAGIAACDGGHIMTVRDLGLVSQVFDEQKLRALTGDMAVGHVRYSTTGSSAWENAQP
                     VYRSDRRQLALAHNGNLINAVELHGELLDKGVSFRSTSDSEIIAALLSTHEADSIEEA
                     LADTLPRLQGAFSTVVMTTDKLIAFRDGAGLRPLSLGRLGDRWCVASESCAFDIIGAE
                     LVREVEPGELVVIDESGMQARQAIESPRKAFCVFEHIYFARPDSILGGNRTQVSRRKM
                     GEILWRESPVVADVVIAVPDSGNPAAAGFSKASGIPRDEGLIKNRYVARTFIQPGQEL
                     RKHGLRMKFNPLREVVEGKRLVVVDDSIVRGNTTRQIVQMLRDAGALEVHMRISAPPI
                     RYPCHYGVDMSTTDEMVAHGRSIEEIAAELDCDSLAYLSLEGVYEAIRGERGTHCDAC
                     FSGEYPLDGTGDAQGKYAFELPVVTGSPS"
     gene            complement(9521..10357)
                     /locus_tag="VHF88_01435"
     CDS             complement(9521..10357)
                     /locus_tag="VHF88_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX2160467.1"
                     /translation="MADSSQQHATDEQMAVVIRRFGEALRVGDPERAIAATDVAIAGG
                     MTAAAAEARIVAPAMQWIGDLWERGAIGVADEHLATAISDSTLAHLYPRLRTATPRSR
                     ERVLVSAVQNERHVLGLRMVADALEGAGFDVLYLGGDVPPGALLDACRKHRPAVVALG
                     VTMHVNLPALLGEVTALAALDDPPQVVLGGQGIPASVRGELSVPVLASSERAVEAVER
                     LLRDPAPVLPPPPALLVRSITEIAPARRELEETDALAERFLAATLSAAETARELERGR
                     PS"
     gene            10430..12115
                     /locus_tag="VHF88_01440"
     CDS             10430..12115
                     /locus_tag="VHF88_01440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX2160468.1"
                     /translation="MELARALQEHFGFSTFRPGQQAACEAALAGRDVLVVMPTGSGKS
                     LCYQLPALMRDDLTIVVSPLVALMQDQVEGLRARGLGDRVALVNAQRDAATNVETLRR
                     AAAGDLSLLYVAPERFASPGFLEAMQDARIGLFVVDEAHCVSQWGHDFRPDYFRLADA
                     ARYLGAGSIVASTATATARVALDIARRLQLDDPVKVSTGFDRPNLSFAVARPGGHEKR
                     ALIAQALSDPDALPATVYAGTRAGTEEIADQLSEALGEPALAYHAGLDRETRANVQRR
                     FLADDARVIVATNAFGMGVDKPNVRSVIHASVPSSLEAYCQEAGRGGRDGLPARALLL
                     AENRDKALHVHFIKREEVDERLPAALYERLRAVLGHLTRAGAISPSPSSPDRVAGKIV
                     APYERLVAAQCRRSVEEGASARWLQYREIWAYVEGDKCRRTAILRHFGDQRNGDSSPG
                     PCCDVCVEGLIPTLPPMAQHEVASLDDAILSIARLAKPAIGRTTCAAIVHGARSQKIE
                     RNSYDGLPGYAAAPHMRRADILARVDELIAAGSIEVSSGIRPTLSVPAARLAA"
     gene            12121..12741
                     /gene="purN"
                     /locus_tag="VHF88_01445"
     CDS             12121..12741
                     /gene="purN"
                     /locus_tag="VHF88_01445"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX2160469.1"
                     /translation="MAFRVAVLISGEGSNLQALLDRVHGREGIEVACVASSRAGARGL
                     ERAAVAGVDSAVFARDDHPDRDARDSALGDWLEGHDVDLVVLAGFMEILGGPFIRRFE
                     GRLINVHPSLLPAFPGVRAIEQALDYGVQVSGVTVHFVDEGIDTGPVVLQEAFELPYA
                     RAVEEVERVTHEVEHRLLPRAVELIARGAVSRDPDNPRVVRVREEM"
     gene            12738..14324
                     /gene="purH"
                     /locus_tag="VHF88_01450"
     CDS             12738..14324
                     /gene="purH"
                     /locus_tag="VHF88_01450"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX2160470.1"
                     /translation="MSAESEVAAAGGVRVRRALLSVSDKRGLVDFARGLQALGVEIVS
                     TGGTASTLADAGIETRSVEDYTGFPEILDGRVKTLNPRIYAGLLAVRSNPEHSATLEQ
                     QQIEPIDLVCVNLYPFERVSGRRGVDEDEVIENIDIGGPTLIRAAAKNHRFSAVIVSP
                     ESYDAVLAELESSDGHLEAPTREALALEAFSYTARYDAAISGWFAEREEDFPGQITRS
                     FVKVLDLSYGENPHQRAAYYADARARTHLLSMVSKLHGKELSFNNLLDLDSGRRLIEE
                     LELPAAVIIKHNNPCGCAVGSSVEEAFEKALATDPLSAFGGVMCFNRPIERGLAERLN
                     SMFVELVFAPSFEGDALEVLKQKPNLRLLEDKERRRPAISEQDFKRVRGGILVQDRDL
                     SAEAREDMLAVTERKPTEAEWGELLFAWRVAKHVRSNAIVLVRELATAGIGAGQMSRV
                     DSVRLAVEKARDAERPLDGAVLASDAFFPFADGPQLAIDAGIRAIVQPGGSQRDPEVV
                     AACDAAGIAMVFTSRRHFRH"
     gene            complement(14440..14513)
                     /locus_tag="VHF88_01455"
     tRNA            complement(14440..14513)
                     /locus_tag="VHF88_01455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14477..14479),aa:Lys,seq:ttt)
     gene            complement(14557..14628)
                     /locus_tag="VHF88_01460"
     tRNA            complement(14557..14628)
                     /locus_tag="VHF88_01460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14593..14595),aa:Glu,seq:ttc)
     gene            complement(14679..15026)
                     /locus_tag="VHF88_01465"
     CDS             complement(14679..15026)
                     /locus_tag="VHF88_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160471.1"
                     /translation="MDKGLFIRSLAVQAIAVAIVFAALLALPLGDDFFEDYGWITGPL
                     AWIACSLVTARVLSIPAGLVLFAALAGGVAGALVALVTSHIVGLVISLLVFAASCGGY
                     DPERDERDAAATA"
     gene            15078..15150
                     /locus_tag="VHF88_01470"
     tRNA            15078..15150
                     /locus_tag="VHF88_01470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15111..15113,aa:Val,seq:tac)
     gene            15636..16088
                     /locus_tag="VHF88_01475"
     CDS             15636..16088
                     /locus_tag="VHF88_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160472.1"
                     /translation="MASPQVNLRLSESDIQRLDQLAGGLGLSRTETVRFALYELQRSD
                     PVRRRNRFAEMLREQFGHDALLRVELDRGFDVLGTVDGEHQPDLYLPTFPTRFGDDDF
                     VQVWIGDPATDDVRILLGLIPMRTGVHLDVPLAELNAAMAPKAVAWFT"
     gene            16076..16735
                     /locus_tag="VHF88_01480"
     CDS             16076..16735
                     /locus_tag="VHF88_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160473.1"
                     /translation="MVHLMRESMSTQRISPNALLAFKEALSAVFWAKKDLLAWLQSAV
                     PDAALLDGIDWLGPDYKRDSVARFVDRLARDQSRHGELLIRLMVDIAAMEDFPRLAWT
                     DDAERLIDEAQEAVGRLRQYMKPYEAQLVEQEVARDRIQQARADAQAQRATAGALERL
                     KMQFFELHGMEDAQARGHLFEPLPSRLVRCLRPSTPRGISDRGRADRRWIYVGPAPLP
                     A"
     gene            complement(16914..17369)
                     /locus_tag="VHF88_01485"
     CDS             complement(16914..17369)
                     /locus_tag="VHF88_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160474.1"
                     /translation="MVEAAVADCELAPGEQTPSGAPSEPVSFDVDPESERQLRERPEN
                     RPTASSGTAAAQEPYTLKYGPTDITDTSALTGRQYNDFKQTPELAEKFVDPPTPDLEP
                     GPDCGPQRRLAQGSEDLTSWPVDLSRHRRVTPLRLKKIDQVNPLLNNSE"
     gene            17805..18917
                     /locus_tag="VHF88_01490"
     CDS             17805..18917
                     /locus_tag="VHF88_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HEX2160475.1"
                     /translation="MTEPVLWNLDSHTAAKHRVLRSYLDGWIPVMAQQAQRVRSYGGE
                     PRLLMIDGFAGPGRYATGQEGSPLIMLDALLSHKAFPRLADVQFFFFFIEHDGRRVDR
                     LRDEVASLGQLPRNVSVEIAHGEFETTLGSLIDPITDSGKALIPTFAFIDPFGYSSAS
                     MSLTGRLLDFPRCEVLFFLPLSFVHRFVGRDGQDVALNSLFNTDEWREAIALSGADRR
                     QFLLELFERQLGQMASVEHVRSFQLRTQDGNDYRLVFGLGHTKGLELAKDAMWGVDPV
                     GGTSYTATTEAGQEVLFAPDDLVDTAPLLRELRAKFGDAWFTIEQAEECTLIDTPFRK
                     GHLRQRTLQPAEREGVIEVQRPGSRGFRDAKLRFVD"
     gene            19027..19755
                     /locus_tag="VHF88_01495"
     CDS             19027..19755
                     /locus_tag="VHF88_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HEX2160476.1"
                     /translation="MADNSAIEWTEATWNPVTGCSQVSPGCAHCYAKTFAERWRGVPG
                     HPYEQGFDLRLWPHRLEQPLRWTRPRMIFVNSMSDLFHEGIPLDYIEQVFEIMGQAEH
                     HTFQILTKRHERLAELAPQLPWHPNIWIGVSIENRRFVDRADYLREVNAAVRFISAEP
                     LLGPLTGLDLSGIDWLIAGGESGPKHRPVRVEWLRDLRDQCVDEEVAFFFKQWGGHRP
                     KSGGRALDGCTWDEMPVARVAQLT"
     gene            20021..20245
                     /locus_tag="VHF88_01500"
     CDS             20021..20245
                     /locus_tag="VHF88_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2160477.1"
                     /translation="MHQARTHLSQLVERALEGEEIVLTRRGEPVIRLVPEQPGGGFAA
                     LAGVWRDRVRIADDFDGLSEDLAEPLGMDS"
     gene            20316..20972
                     /locus_tag="VHF88_01505"
     CDS             20316..20972
                     /locus_tag="VHF88_01505"
                     /inference="COORDINATES: protein motif:HMM:NF020922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX2160478.1"
                     /translation="MATFRGFPSEALGFLAELEANNDRDWFKANRGRYDEALVAPATA
                     LGEDLADLGRPKLFRPWNDTRFHAAPPIKEHVGLAIGYEGAGGFYVELSLDGLLVAAG
                     LHNPASDQVERMRRAIDGARTAGALGKAIRHAEAAGLELNGPDLVRGPRGYPADHPRL
                     DLLRRRRLVVSKRQRTGRWLERPAAGKRIREQLEAAAPLVRWLRTHVGPSERGAARAG
                     "
     gene            20982..>21530
                     /locus_tag="VHF88_01510"
     CDS             20982..>21530
                     /locus_tag="VHF88_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2160479.1"
                     /translation="MRVLSGLLFFPRGGSAQVARALATELPRQGWDVSVLSGSLGSGR
                     ADARQFYSGLDVHSVDFDAGDAPMHPSYEDRPDAPDRCFAMVDDEAYREHVAAWARAM
                     ERAGAASADVLHLHHLTPLNEAAALAAPGVPIVGHLHGTELMMLERIAAGPPPEWTHA
                     DAWAKRMRRWAQGCERLILLADS"
BASE COUNT         3216 a         7428 c         7540 g         3346 t
ORIGIN      
        1 cgaccatcct gctcgactac gcgcagcacc tcgctatccg cgtcgtggac gggatgacag
       61 tccatgagga ccgcatgcgc gagaacctca acctgacgca cggggcgctc tactcccagc
      121 gggcgctgct cgcgctcgtg gagagcggcc gctcgcgcga cgacgcctac cgcatcgtcc
      181 aggagaacgc ccagcgcgcc tgggacgagg gcatcgagtt tcgcaatctg ctcgccggcg
      241 ccgggctcga gcgcttcgcc accgagctgc gccacctcca gcgcaccgag gtgctcgagg
      301 cgcgcgagcc gttccgcgcc ggccagaagg gctcgtcggc gatgccgcac aagcgcaacc
      361 cgatcgtctc cgagcggatc accggcatcg cccggctgct gcgcggctac gcccaggccg
      421 ggctcgagga cgtcgcgctc tggcacgagc gcgacatctc ccactcctcg gtcgagcgcg
      481 tcgccctgcc cgacgcgacg atcctgctcg actacgccca ccacctcgcg acccgcgtcg
      541 tcgaggggat ggtcgtcaac gccgatcgga tgcgcgccaa catcgacctc acccacggcg
      601 cgctcttctc ccagcgcgcg ctgctcgcgc tcgtcgagtc cggccgctcg cgcgacgacg
      661 cctaccgaat cgtccaggag aacgcccagc acgcctggga cgaagggatc gagttccgcg
      721 acctgctcgc ggaggccgcg cccgagctcg acctcgacgc gatcttcgac ccgaccgcct
      781 tcacccgcca cgccgccgac atcgtcgccc ggctagacag cctcgactga ccggggccgc
      841 cggcctcact ggtagcctcg cgggagtgac ggaagccgcc gcgctcgcag acctccagcc
      901 cgttgcccag gggaaggtcc gcgacatcta cgcggtcggc gaggaccggc tgctgctggt
      961 cacctcggac cgcatctcga cctacgacgc ggtccatccg acgccgatcc cggataaggg
     1021 aaaggtgctg acggggctgt cgtgcttctg gttcgatcgg accgagcaga tcgtccccaa
     1081 ccacctcgtc tcctataccg aggtgccgga cgaggtgcgt gggcgcggcc tgctcgtcga
     1141 gcgactcgag atggcccagg tcgagtgcgt cgtgcgcggc tacatcaccg gctcgggttg
     1201 gaaggactac caggcgagcg gcgcggtctg cgggatcgag ctgccgtcgg ggctgcagga
     1261 gtccgagcag ctgcccgagc cgatcttcac gccggcgacg aaggccgcgg tcggcgacca
     1321 cgacgagaac gtcgacttcg accgcgccgc cgagatcctc ggcgaccgcc cgctgctcga
     1381 ggagctgcgg cggctgtcgc tggcgctcta cgagttcggc gccgcgcacg cgcgcgagcg
     1441 cgggatcatc cttgccgaca ccaagttcga gttcgggcga cgtcccgacg ggacgatcgt
     1501 gctcgccgac gaggtgctga cccccgactc ctctcgcttc tggcccgccg acggctacga
     1561 gcccggccgc ggccagccga gcttcgacaa gcagttcgtc cgcgactggg cctccggctc
     1621 gggctgggac aagtcgccgc ccgcccccgc gatccccgac gacgtgatcg ccggcacgcg
     1681 cgagcgctac gtcgaggcgt acgagcggat cgccgacgag ccgttctcgg cctggctgga
     1741 gcgcagccgg agcgctgggt gagggcgcgc gtcctgatcc gccccaagga gggcatcctc
     1801 gatccccagg gccaggcggt cgagcgggcg ctgccggcgc tcggcttcga gggcgttgcg
     1861 aacgtccacg tcggccggct ggtcgagctc gacgtcgaca gcgtcgacgc ggtccccgag
     1921 atgtgcgagc ggctgctggc gaacccgctc atcgaggact acgaagttct cgcggtcgag
     1981 tgaagttcgg cgtcgtcagg tttcccggct cctgcgacga ggtcgacgcg ctgcttgcct
     2041 gcgagcagca gggcgaggcc gagatcctct ggcacggcga ccgcgacctg aagggcgtcg
     2101 acgccgtcgt gatcccgggc ggcttcagct acggcgacta cctgcgcgtg ggcgcaatcg
     2161 cgcgcttctc gccggtgatg gaggcggtcg cggagttcgc ccgtgacggc ggaccggtgc
     2221 tgggcatctg caacggcttc caggtgctct gcgaggcggg gctgctgccc ggcgcgctgc
     2281 tgccgaacac cgagctgcgc ttcctctgcc gccaggtcga cgtgcgggtc gtcaaccctg
     2341 acaccccctt cacgcgcgcg tgcgatgtcg accagaccct ctcgatcccg gtcaagcaca
     2401 ccaccgggcg ctactacgcg cctgacgcga cgatcgccga gatggaggac gtcggtcagg
     2461 tcgtcttccg ctacgccgag agctggaacc cgaacgggtc ggtgcgcgac atcgccggcg
     2521 tctgcaaccg cgagcgcaac gtcgtcggcc tgatgcccca ccccgagcac gccgtcgatc
     2581 cgctgaccgg ctctgccgac ggggcctgcc tgttcgcctc cgcggcgcag atggtggccg
     2641 cgtgagcacc gaggcttccg cgccggacct gcgcgcagag gcacgcgccc tcggtctcac
     2701 cgactccgag tacgacctga tcggcgccaa gctcggccgc ccgccgaacg ggctggagct
     2761 ggcggtgttc tcgctgatgt ggtcggagca ctgcgcctac aagcactcca agaagctgct
     2821 cggccggctg ccggtcgagg gtccgcacct gctgatggga ccgggcgaga acgcgggcgc
     2881 ggtcgacgtc ggcgacgggc tggcgatcgc cttcaaggtc gagtcgcaca accacccgag
     2941 cgcggtcgag ccgttccagg gggccgccac cggcgtcggc ggcatcatgc gcgacatctt
     3001 cgccgtcggc gcccggccga tcgccgtcct cgactcgctg cgcttcggcg agctcgactc
     3061 gccgcgctcg cgctacctgc tcgacggggc ggtgtcgggg atcggccact acggcaactc
     3121 gatcggcgtc gcgaccgtcg gcggcgaggt ctacttcgag ggtccctacg agcagaactg
     3181 cctcgtcaac gcgatgtgcc tcgggatcgc cccgcaggag cgcctgacgc gcagcgccgc
     3241 ggcgggcgtc ggcaaccacc tcgtgctgat gggtgcgcgc accggacgcg acgggatcgg
     3301 cggcgcctcg gtgctggcga gcgccgagct cgacagcgag gacgccgaca agcggccgac
     3361 cgtccagatc ggcgacccgt tcgaggagaa gaaggtgatg gaggcctgcc tcgagctgct
     3421 cgaccgcgag ctgctcgcct cgctgcagga cctcggcgcg gccggcctgt cgtcgtcctc
     3481 gtccgagatg gcgtcgaagg gcgaggtcgg gctcgacatc gacgtctcga gggtgccgct
     3541 gcgcgaggcc gacatggagc cgttcgagat catggtctcc gagtcccagg agcggatgct
     3601 ggccgtcgtc gagccggagc agctcgccgc ggtcgaggcg gtctgcgagc gctgggaggt
     3661 ccgctgcacc gtgatcgggt cggtgaccga cagccgccac ctgcgcgtct tcgacggcga
     3721 cgagctggtc ggcgacatgc cggtcgacgc gctcgtcgat gactgcccgc tctacgacct
     3781 cgcacccgag cagcccgtcg agcagatcta tcccgcgccg cgccgcacgc tggcgccgag
     3841 cgacgacctc gccgagaacc tgctcgcgct gctcggctcc tcgaacgtcg cctcgcgccg
     3901 cccgctgttc gagcagtacg actcgctcgt ccagtcgcgc acggtgcgcc gtccggaggc
     3961 cgccgatgct gcggtgctcc agctcaagca cgacaccggc ggcgggtcga gcgcgatcgc
     4021 cgtctcgatc gacggcaacg gccgccgcgt cgcctgcgac ccctactggg gcaccgtcga
     4081 ggcggtgctc gagtgcgcgt cgaaccttgc ctgcgtcggt gccgagccgc tcggcctgac
     4141 caactgcctc aacttcggca accccgagaa gccgcacatc gcctggcagc tctcgcgctc
     4201 ggtcgacggc atggccgacg cgctgcacca gctcggcgtc ccggtcgtcg gcggcaacgt
     4261 ctcgctctac aaccagtccg gcgacggccc gatctatccg accccggtcg tcggcatggt
     4321 cggcctcgtc cccgatgccg cgcgcgccgc ggcggtcggt ctcggcttcc gcgaggaggg
     4381 ccacacgatc gcgatggtcg gcacgccgca gccctcgctc gccggctccg agctcgccaa
     4441 gctgcgcggc gagccgctgc ccacctcgct gccggagatc gacttggccg cagccaagcg
     4501 cgcccaggag gcggtccgcg atgccgtccg cgcgggcggc ctcgcctccg tccacgacat
     4561 cgcggagggc ggcttcctcg tcgcgatcgc ggagtgctgc ctcgccggcg acatcggcgc
     4621 ccggctcgac ttcggctctg ccgacgtcga ttgggcagcg cttgagccgt acctgttcgg
     4681 cgagggtgtt ggcggcttcg tggtctcggg cgagcgcgag gcgatcgacc gcctggccga
     4741 gcgggttccc acggatgtct tcggcaaggt cggcggcgac gcgctctggg tcggccttgg
     4801 cggctcggcg ccgcgggtcc cgctcgcgcg cctgcacgcc gctcacggag cgctcgccgc
     4861 ggcgttcccc tagcgctcgt tcgcgcgggc tccgctacgc cgcggcggtc aggcggcgag
     4921 cgcccgcccg agcgacgtcg aggatcgcct cgaccacctg ctcgcgcggc acgtcgcgat
     4981 gctggtacca ccacgccgcg aaggcgcgga tcgcagtcgc cgtgaggtcg aggtgcgggt
     5041 cctgtcgcag gcgcttgccc ggggtcgcaa gcagctcggc gagcgccgcc gcgcgctgct
     5101 cggacaggcg ctcgtggacc gcgatcaggg ccggctcggc gggcggatcg cgcagcagca
     5161 gccgccacgc cgccgggcgc tcggcgagga agccgagata ggcctcgacg cccggccgta
     5221 gccgcgatgc gcccgggcgg gtcggaactg cgtcgcgcac ccgctcgatc aggtcgcgcg
     5281 tctcggcttc gacgatcgcc tcgtagagct gggccttgga gccgaagtgg tggtagagca
     5341 cagacttggc gatccccgcc cgctcaccga tgtcggcgat cgaggtcgcg tggtagccgc
     5401 gctcggcgaa cagctcccgg cccgcggcca ggacgcgctc gccgccggcc tcctctgggc
     5461 ggaccttggc tcggcgcggc atcgcccgag gatatcgacc gtgcggtcga aacactgcta
     5521 cggttttaga ccggccggtc taaaccaact cggatggagt gacatggcaa gtgcgaggac
     5581 gatcctgatc agcggcgcct ccggcgcgat cggagccgac gtggtgcgct cgctcgcccg
     5641 ccgcggggcc cgcctggcga tctcagcgcg gcgtgcggac atcctgatgg cgctggccga
     5701 tgagatcgag gcggcgggcg ggatccgccc gctggtgctg agcgcggacc tctcgcagcg
     5761 cggggaggcg ggcgagctgg cgcggcgggc gaccgacgcg ctcggcgacg tcgacgtgct
     5821 ggtcaacaac gccggcgcga gcgtccaggg cctgatcgcg acggtcgccg accgcgacga
     5881 ggcgcgccac gtcttcgaga cgaacctctg gagcccgctg gcgctcgtcg ccgagctggc
     5941 gccgcgcatg ctcgagcgcg gcgcggggac gatcgtcaac gtcggctcga tggtccgggt
     6001 ctcgccgtat ccgcatctcg gtcactacgc ggcgtcgcgt gcggccctcg cactcgcaac
     6061 ggaggtcctt gagctggagc tcgctccgcg cggggtgcgc gtcgtcgagg tcgcgctcgg
     6121 accgatcgac acgcccgcgt cgcgcgagaa ccgcctgctg gcaggcgcgg accgctggct
     6181 cgacggccgc cccggcgtcg gccgggccga cgcggcggcg cgagcgatcg tcgccgcggt
     6241 cgaaggcgat gcgcggggcg tcgtcttcca tccgcgctcg ctgcgctggg ccgacaggct
     6301 ccccgctctc gggcggcgct actcgcggcg catggcgcgc ggcgcggatc tcttcgacca
     6361 cacggtgcgg ctgggcggct cggcgggcga ccccgagctg caggcggtgc accgggactg
     6421 ggcagccact gcgggcgatc cgcgctgagg ggcatggccc ggacgaccga gcgacgtcgc
     6481 cgtgaccgat tttgatagat gtgccgcgat gggcatgcag ggggcgcaag ccgagttgcg
     6541 cggtcgctgg aggcgttggc tcgcgagccc agttccgctg acgctgttgg cggccgccgt
     6601 gctgccggcg tcggcgagcg cggcgccgga cttcaaggtc gcgctgccga gcgccggcga
     6661 catgaccgtc gcggtcgtca acttcaaggt cgacccaccg aaggacaagc gcaagctcgg
     6721 aaagctggtc ggcgatctga cgctggagac gaccgcaaag aagaagaaga agctcgacgc
     6781 cgccgtgctc agcagcctgg tcggccggcg ggtcggcgac aagctgaagg tgaccggcct
     6841 cgtcacgctc gtacggcccg aggacgcgaa ggaggcgtcg agcgagaagc tgaagaagct
     6901 cggcttcgag ctcaccgatg gaatgccgag ctccgcggag aagctcgcga agctgctcgc
     6961 gcaggagcgc aagcgcaagg agcagctcgc gaagcagctc caggtgctga agctcgagca
     7021 ccagaagctc gcgaagcagc tcgtccagcc gggagcgaca ggtttcgagg acgtgaacct
     7081 gcgcctgctc gaggtgttgc agctcattcg cgaggtgacc gagacccgcc gcgaggtcga
     7141 gcggcgtgta cgcgagctcg acaccgagct ccggaagcag cagctgctcg aggccgccaa
     7201 gcagctgaag cggtccgagg gcggcctcgg cgaccccaag gtcgtcgggg acgtcaagaa
     7261 gtccgagtcc aagaacgcgc tcgagaaccc gaagaagacc ggtgaggtgc ttgccaagac
     7321 caagctcgcc gggaagctga tcaagaagct cgacgacgag cattccaagc agcgcgtcga
     7381 cgagaaggag cttggcaagg cgatcatcca ggcgttcctc gacaagaagg acacggagaa
     7441 gcaggaccag gcgctcgaga agctcaagcg ctcgctcgag tcgcagaagt gcaagaaggg
     7501 cgcgctgatc ctgctatgcg aggaccagaa accgccgacg ctcgagaccc ctaacgcccc
     7561 gccgtcggcg ccgcccaagc tgacgccgac tccgggtccg aacgacccct tgccgcctcc
     7621 ggacgagccc gcccccgggc ccgagctggc gatctcgctc tcgcacagcg acgagggctg
     7681 gttcaacttc tgcatcgaca tcgcgaccac tccgccgcag ccgggcgcgt cgggcacgta
     7741 ctctttcggc gagagcggca ccgaaggctt cggccccgag ccgttcgtgc tcgacgagaa
     7801 cggtcgcgcc aaggtcgtgt tcgagggccc cgatccggcg gtcgtcgatt ccttcttcgc
     7861 cgaggtgacg ctcgactggc caggcatcgg cccgatcacc gagcggatcg agggcccggg
     7921 ctgggggtca ctcgtcggca cggacgagtg ctcgatcgat ctcgaaggct gagcgcgcca
     7981 ccacccagcg gtcccagaga ccgccgccat cgctgttacc ctgggacgtc cgtgccgggc
     8041 cccactccgg cctgccgcgg gcaacgttcc cctggaccgg tctcatgacc tcccatccga
     8101 tctccgatcg cgacgggccg cgcgacgagt gcggcgtgtt cggcgtactc gcgcccgagt
     8161 ccgaggtcgc gcggctggcc tacttcggcc tcttcgcgct ccagcaccgc ggtcaggagt
     8221 cggccgggat cgcggcctgc gacggcggcc acatcatgac cgtgcgcgac ctggggttgg
     8281 tgtcgcaggt cttcgacgag cagaagctgc gcgcgctgac cggcgacatg gcggtcgggc
     8341 acgtgcgcta ctcgacgacg gggtcgtcgg cgtgggagaa cgcccagccg gtctaccgct
     8401 ccgaccgccg ccagctcgcg ctcgcccaca acggcaacct gatcaacgcc gtcgagctgc
     8461 acggcgagct gctcgacaag ggcgtctcgt tccgctcgac ctctgactcg gagatcatcg
     8521 ccgcgctgct gtcgacccac gaggccgact cgatcgagga ggcgctcgcc gacacgctgc
     8581 cccggctgca gggcgcgttc tcgacggtcg tgatgacgac cgacaagctg atcgcgttcc
     8641 gcgacggcgc cggcctccga cccctgtcgc tggggcggct gggagatcgc tggtgcgtgg
     8701 cctccgagag ctgcgcgttc gacatcatcg gcgccgagct ggtgcgcgag gtcgagccgg
     8761 gcgagctggt cgtcatcgac gagtccggga tgcaggcacg ccaggcgatc gagtctccgc
     8821 gcaaggcgtt ctgcgtcttc gagcacatct acttcgcgcg accggactcg atcctcggcg
     8881 gcaaccgcac ccaggtctca cggcgcaaga tgggcgagat cctctggcgc gagtcgccgg
     8941 tcgtggcgga cgtcgtgatc gccgtgcccg actccggcaa cccggccgcc gcgggcttct
     9001 cgaaggcgtc cggcatccct cgcgacgagg gcctgatcaa gaaccgctac gtcgcacgga
     9061 ccttcatcca gccggggcag gagctgcgca agcacggcct gcggatgaag ttcaacccgc
     9121 tgcgcgaggt cgtcgagggc aagcggctgg tcgtcgtcga cgactcgatc gtgcgcggca
     9181 acaccacgcg ccagatcgtc cagatgctgc gcgacgccgg cgcgctcgag gtccacatgc
     9241 ggatctccgc gccgccgatt cgctacccgt gccactacgg cgtcgacatg tcgaccaccg
     9301 acgagatggt cgcccacgga cgcagcatcg aggagatcgc cgccgaactg gactgcgact
     9361 cgctcgccta cctctcgctc gagggcgtct acgaggcgat ccgcggcgag cgcggcaccc
     9421 actgcgacgc ctgcttctcg ggcgagtacc cgctcgacgg caccggcgac gcgcagggca
     9481 agtacgcctt cgagctgccc gtcgtcaccg gcagcccgag ctagctcggc cggcctcgct
     9541 ccagctcgcg cgccgtctcg gcggccgaca gcgtggcggc gaggaaccgc tcggcgagtg
     9601 cgtcggtctc ctcgagctcc cggcgcgccg gcgcgatctc ggtgatcgag cggacaagca
     9661 gcgcgggcgg gggcgggagg accggcgccg ggtcgcgcag cagccgctcg acggcctcga
     9721 ccgcgcgctc gctgctcgcc aggaccggga cgctcagctc cccacggacc gacgccggga
     9781 tgccctggcc gccgaggacg acctgcggcg gatcgtcgag cgccgcgagc gctgtcacct
     9841 cgccgagcag cgcaggcagg ttgacgtgca tcgtcacgcc gagcgcgacc acggccgggc
     9901 gatgtttgcg gcaggcgtcg agcagggcgc ctggcgggac gtcaccgccg aggtagagca
     9961 cgtcgaagcc cgcgccctcg agcgcatcgg cgaccattcg caggccgagc acgtgccgct
    10021 cgttctggac cgccgagacc agcacgcgct cacgcgagcg cggggtggcg gtgcgtaggc
    10081 gcgggtagag gtgggcgagc gtgctgtcgc tgatcgcggt ggcgaggtgc tcgtcggcga
    10141 cgccgatcgc gccgcgctcc cagaggtcgc cgatccactg catcgccggc gcgacgatcc
    10201 gcgcctcggc cgccgcggcg gtcatgcccc ccgcgatcgc gacgtcggtg gcggcgatcg
    10261 cgcgctctgg gtcgccgacg cgaagcgcct cgccgaagcg gcgaatgact acggccatct
    10321 gctcgtccgt ggcgtgctgc tgcgaagaat cggccaccga taacagtgtc gccacttcgt
    10381 tgcagatcgc aaccttcacg gccgggagcg ccggtactgt ggccggacga tggagctcgc
    10441 ccgcgccctc caggagcact tcggcttctc taccttccgg cctggccagc aggcagcgtg
    10501 cgaagcggca ctcgccggcc gcgacgtgct ggtcgtgatg ccgaccgggt ccggcaagtc
    10561 gctttgctac cagctgcccg cgctgatgcg cgacgacctg acgatcgtcg tgtcgccgct
    10621 ggtcgcgctg atgcaggacc aggtcgaggg cctgcgcgca cgcgggctcg gcgatcgcgt
    10681 cgcgctcgtc aacgcccagc gcgacgccgc gaccaacgtc gagacgctgc gccgcgccgc
    10741 ggccggcgat ctcagcctgc tctacgtggc gcccgagcgg tttgcctcgc cgggcttcct
    10801 cgaagcgatg caggacgcgc gcatcgggct cttcgtcgtc gacgaggcgc actgcgtgtc
    10861 ccagtggggg cacgacttcc ggcccgacta cttccgcctc gccgacgccg cgcgctacct
    10921 cggcgccggc tcgatcgtcg cctcgactgc cacggccacc gcgcgcgtcg cgctcgacat
    10981 cgcccgccgc ctccagctcg acgacccggt caaggtctcg accggtttcg accggcccaa
    11041 cctttcgttc gcggtcgccc ggcccggcgg ccatgagaag cgggcgctga tcgcccaggc
    11101 gctctccgac cccgacgcgc ttccggcgac cgtctacgcg ggcacgcgcg ccgggaccga
    11161 ggagatcgcc gaccagttga gcgaggctct cggcgagccg gcgctcgcct atcacgccgg
    11221 cctcgaccgc gagacgcgcg cgaacgtcca acgccgcttc ctcgccgacg acgcgcgcgt
    11281 gatcgtcgcg accaacgcct tcgggatggg cgtcgacaag ccgaacgttc ggtcggtgat
    11341 ccacgccagc gtcccgtcgt cgctcgaggc ctactgccag gaggccggcc gcggcgggcg
    11401 cgacggcctg ccggcgcgcg cgctgctgct cgccgagaac cgcgacaagg cgctccacgt
    11461 ccacttcatc aagcgcgagg aggtcgacga gcggctgccc gcggccctct acgagcgcct
    11521 gcgcgccgtg ctcggccacc tcacgcgcgc cggcgcgatc tcgccgtcgc cgtcgtcgcc
    11581 cgaccgcgtc gccggcaaga tcgtcgcccc ctacgagcgc ctcgtcgccg cccagtgccg
    11641 ccgctcggtc gaggagggcg ccagcgcgcg ctggctccaa taccgcgaga tctgggccta
    11701 cgtcgagggc gacaagtgcc gccgcaccgc catcctgcgc cacttcggcg accagcggaa
    11761 cggggactcg tctcccggcc cctgctgcga cgtatgcgtc gaaggcctca tcccgacact
    11821 ccccccgatg gcccaacacg aggtcgccag cctcgacgac gcgatcctct ccatcgcccg
    11881 gctcgccaag ccggcgatcg gccgcacgac ctgcgcggcg atcgtccacg gcgcccgcag
    11941 ccagaagatc gagcgcaact cctacgacgg cctgccgggc tacgcagccg ctccccacat
    12001 gcgccgcgcc gacatcctcg cgcgcgtcga cgagctgatc gcagccggct cgatcgaggt
    12061 gtcgagcggg attcgaccga cgctcagcgt gccggccgcg cggctggccg cctgatcgcg
    12121 atggcgttcc gcgtcgcggt cctgatctcc ggcgagggca gcaacctgca ggcgctgctc
    12181 gaccgcgtcc acgggcgcga ggggatcgag gtcgcctgcg tcgcgtcgag ccgcgccggc
    12241 gcgcgcgggc tcgagcgggc ggcggtcgct ggtgtcgact cggctgtctt cgcgcgggat
    12301 gaccaccccg atcgcgacgc gcgcgactcg gcgctcggcg actggctcga gggccacgac
    12361 gtcgatctcg tcgtgctcgc gggcttcatg gagatcctcg gggggccgtt catccgccgc
    12421 ttcgagggcc ggctgatcaa cgtgcatccg tcgctgctgc cggcgttccc cggcgtgcgt
    12481 gcgatcgagc aggcgctcga ctacggagtc caggtcagcg gcgtgaccgt ccacttcgtc
    12541 gacgagggga ttgacacagg gccggtcgta ctgcaagagg ccttcgagct tccatatgct
    12601 cgcgccgtgg aggaagtcga gagggtgacg cacgaggtcg agcaccgcct gctgccccgt
    12661 gccgtcgagc tgatcgcccg cggcgcggta tcgcgcgacc ccgacaaccc gcgcgtcgtg
    12721 cgcgtgcggg aggagatgtg agcgccgagt cggaagtcgc ggcggccggg ggcgtgcgcg
    12781 tccgccgcgc gctgctgtcg gtttccgaca agcgcgggct ggtcgacttc gcgcgcggtc
    12841 tgcaggcgct cggcgttgag atcgtctcga ccggcggcac cgcctcgacg ctcgccgacg
    12901 cgggcatcga gacccgctcg gtcgaggact acaccggctt cccggagatc ctcgacggcc
    12961 gcgtcaagac gctcaatccg cgcatctacg ccggtctgct cgccgtccgc tcgaacccgg
    13021 agcacagcgc gacgctcgag cagcagcaga tcgagccgat cgacctcgtc tgcgtgaacc
    13081 tctacccgtt cgagcgggtg tcggggcggc gcggcgtcga cgaggacgag gtgatcgaga
    13141 acattgacat cggcgggccg acgctgatcc gtgccgccgc caagaaccac cgcttctcgg
    13201 ccgtcatcgt cagccccgag agctacgacg ccgtgctcgc cgagctcgag tcctcagacg
    13261 gccacctcga ggccccgacg cgcgaggcgc tcgcgctcga ggcgttctcc tacacggcgc
    13321 gctacgacgc cgcgatctcc ggctggttcg ccgagcgcga ggaggacttt cccggccaga
    13381 tcacccgctc gttcgtcaag gtcctcgacc tctcttacgg cgagaacccg catcagcgcg
    13441 ccgcctacta cgccgacgcg cgcgcccgca cgcacctcct gtcgatggtc tcgaagctcc
    13501 atggcaagga gctctcgttc aacaacctgc tcgacctcga ctccggccgc cggctgatcg
    13561 aggagctcga gctgccggcc gccgtgatca tcaagcacaa caacccgtgc ggctgcgcgg
    13621 tcggctcgag cgtcgaggag gccttcgaga aggcgctcgc gactgatccg ctgagcgcct
    13681 tcggcggcgt gatgtgcttc aaccggccga tcgagcgcgg cctcgccgag cgcctcaact
    13741 cgatgttcgt cgagctcgtc ttcgcgccct cgttcgaggg cgacgcgctc gaggtcctca
    13801 agcagaagcc gaacctgcgc ctgctcgagg acaaggagcg gcgccggccg gcgatcagcg
    13861 agcaggactt caagcgggtc cgcggcggca tcctcgtcca ggaccgcgac ctcagcgccg
    13921 aggcccgcga ggacatgctc gccgtcaccg agcgcaagcc gaccgaggcc gagtggggcg
    13981 agctgctgtt cgcctggcgc gtcgccaagc acgtccgctc gaacgcgatc gtgctcgtgc
    14041 gggagctcgc gaccgcgggg atcggggccg gccagatgag ccgcgtcgac tcggtgcgtc
    14101 tggcggtcga gaaggcccgc gacgccgagc gcccgctgga cggtgcggtc ttggcctcgg
    14161 acgccttctt cccgttcgcc gacggtcctc agctggcgat cgacgcgggc atccgcgcga
    14221 tcgtccagcc gggcgggtcg cagcgcgatc ccgaggtcgt cgccgcctgc gatgcggccg
    14281 gcatcgcgat ggtgttcacg tcgcggcggc acttcaggca ttagggatcg gcggtggtgg
    14341 ccgccgggtc ctgtcagggg gaaatccggg gtgctcaaga gggctgcgcg ccccggattt
    14401 ccccctgcca cccgcgggag gggcgtcggt tggtcgaggt gcccccggtc ggactcgaac
    14461 cgacgccgcg cggcttaaaa ggccgccgct ctaaccagct gagctacggg ggcccggcga
    14521 atgatgccgg accccgctgt ggtggtcgag tgggagtagc cccggggaga atcgaactcc
    14581 cgcctgcggc gtgaaaggcc gctgtcctaa ccgttagacc acggggcctg gagggatgag
    14641 gaccagtcta agcgccgctg tgtgcgtacc gcggtggtct aggcggtagc ggcggcgtcg
    14701 cgttcgtcgc gttcggggtc gtagccccca caggacgcgg cgaagacgag caagctgatc
    14761 acgagaccaa ctatatgcga cgtgacaagc gcgaccaggg cgccggccac tccgccggcc
    14821 agcgctgcaa agaggacgag gccggctggg atcgacagga cgcgtgcggt cactagcgag
    14881 caggcgatcc aggcgagcgg gccggtgatc cagccgtagt cctcgaagaa gtcgtcgccg
    14941 agcggcagtg cgagcagggc cgcgaagacg atcgcgacgg cgatcgcctg gacggccagc
    15001 gaccgtatga acaggccctt gtccacgcgg cccgatacta gggcctgcgc ggacgcggta
    15061 gtttcagtgt cccgcgaggg cggttagctc agctggtaga gcgcctgcct tacaagcagg
    15121 aggtcactgg ttcgatccca gtaccgccca ctctctcggt cccgctctgt aggcgggatc
    15181 gccctttcgg gacggatcgg cagccaagcc ctcgttggag gtcggtgggc caacggggtc
    15241 tgatcgtcgg cccgtacaga gcgcccaggg cggcgctgac gagcgcgtcg aaggcggctc
    15301 gtgggcgcgc ttagagcagt tgagcaactg cgcgtagacg tccatcgtca tcttggaatc
    15361 gggtgcccga cctggcgcag gacgaacggg atgtccaagc tcgtcgccag caacatgatc
    15421 gagacgtagg agcgccggag gatgtgcggc gtgatgtggg aagcggcggt cggccgtggt
    15481 cgaccagccg tgcgctcgcc cgctcggcga cggtgccgac gatgcgactt cggacgttgt
    15541 ggtcgctcca acaggttccg tcgcgggtgc agaagaagaa gttcgtagca ctacgtgcta
    15601 cactaactgc tagcagcgct gctagcatct agatcatggc tagtcctcag gtgaatctac
    15661 ggctgagcga gtcggacatc cagcgcctag atcaactcgc tggtggcctc gggttgagcc
    15721 gaacagagac ggttcgcttc gccctgtacg agcttcaaag gtctgacccg gtgcgtcgac
    15781 ggaatcgttt cgcggaaatg ctgcgcgagc agttcgggca cgatgcgcta ctcagggtgg
    15841 aactagatcg cggcttcgat gtgctgggga cggtagacgg cgagcaccag cccgacctct
    15901 acctaccgac ctttccgacc cgctttggcg acgatgattt tgtgcaggtg tggatcggtg
    15961 acccggcgac cgacgacgtg cggattctcc tagggttgat tccgatgcga acaggggttc
    16021 accttgacgt gccccttgct gaactcaacg cggcgatggc gcccaaggct gtcgcatggt
    16081 tcacctgatg cgggagagca tgtcgacgca gcggatctcg cctaatgctc tcctcgcctt
    16141 caaggaagct ctatcagctg tgttctgggc gaagaaagat ctgctcgcct ggctccagag
    16201 cgcagttccc gacgccgcgc tcttggacgg gatcgactgg ctgggtcccg actacaagcg
    16261 cgattcggtc gcgcgttttg tcgatcgcct ggctagggat caatcgcgcc acggtgagtt
    16321 gctgatccgt ctcatggttg acatcgctgc catggaggac tttccccggc ttgcctggac
    16381 ggatgatgcc gagcgcctga tcgatgaggc gcaagaggca gtcgggcggc tgcggcagta
    16441 catgaagccc tacgaggcgc aactggtaga gcaggaggtg gcccgggacc gaattcagca
    16501 agcgcgtgct gatgcgcagg cacagcgtgc cacagccggc gctctagagc gactgaagat
    16561 gcagttcttt gaacttcatg ggatggaaga tgcgcaagcg cgcgggcacc tgttcgagcc
    16621 ccttccttcg agacttgttc gatgtcttcg acctagcacc ccgcggggca tttcggatcg
    16681 agggagagca gatcgacggt ggatttacgt tggaccggct ccacttcctg cttgaggcca
    16741 agtgggagaa agggctagca acgcgtgccc agctcgacgc cttccgacgc aaggtagatg
    16801 acaaagccga cgccacattt ggtctgttca ttgcgattga gggcttcgag ccgacggccg
    16861 ttgagaagca caacggccgt cgatctccgc ttctcttgat ggacggcggc gatctactcg
    16921 ctgttgttga gcagcggatt gacttggtcg atcttcttga ggcgaagagg cgtcacgcgt
    16981 ctatgacggg agagatctac cggccagcta gtcagatcct cgctgccttg agctagccgc
    17041 cgctgtggcc cgcagtcggg gccaggttcg agatctgggg taggcgggtc gacgaacttc
    17101 tcagctagtt cgggcgtctg tttgaagtcg ttgtactggc ggcctgtgag ggctgaggtg
    17161 tcggtgatgt cggtcgggcc atacttgagg gtgtagggct cttgggcagc tgcggtccca
    17221 ctgctcgcgg tcggccggtt ctcgggacgc tcgcgtagct gccgctcgct ctcggggtct
    17281 acgtcgaatg agacgggttc ggatggcgcg ccactaggcg tctgctcgcc gggtgctagc
    17341 tcgcagtcgg cgacggctgc ctcgaccacc tccagctctt gtcggcgagc cattcgtcga
    17401 agtcgaagtc tgcggcttgg tcggttcggt cggctagatt ttggcgactg tgtcaatcgc
    17461 gcggctgagt tgggcgaggc ggccttctaa gagagcgcgt tggtggcggt cgcgccgccg
    17521 ctggctaagt tggtcgagct cggcttgtgc gtcgtcgcgt agctgcttga tgtgggaggt
    17581 tgctggctgc acgttcggtc aatgccgcgt cttgggcgcg tcgtgcctgg ttggcccggg
    17641 ctcgcgtccg actcgtgcgt cacccatact cggggcgttc gggatcaagc ttgttacggc
    17701 cgcgtgcctt ttgcgtgagt cgcactggtt gaatcaacag acatgactcc tgcatctcct
    17761 ttgtcgcccg caaaccgtcc accccaggcg agatgctccc gctgatgact gagcccgtac
    17821 tttggaatct ggactcgcac accgcagcga agcatcgagt gctccgctcc tatcttgatg
    17881 gctggattcc cgtcatggcc cagcaagcac agcgcgtccg cagctatggc ggcgagccgc
    17941 gccttctgat gatcgacggc ttcgctggtc cagggcgcta tgcaactggg caggaaggtt
    18001 cgccgctcat catgctcgac gccctcctgt cgcacaaggc cttcccgaga ctggcggatg
    18061 ttcagttctt cttcttcttt atcgagcatg acggcaggcg cgtcgaccgt ctccgcgatg
    18121 aggttgcatc gctcgggcag ctaccgcgga acgtgtcggt ggagattgcg catggcgagt
    18181 ttgagacgac gcttggatcg ctgatcgatc ccatcactga cagcggaaag gccctgattc
    18241 caacgtttgc cttcatcgat ccgttcggct actcgagcgc atcaatgtcg ttgaccgggc
    18301 ggttgctcga cttccctcgc tgtgaggtgc tgttctttct gcccctgtcg tttgtccacc
    18361 gcttcgttgg gcgcgacggc caggatgtcg cgctgaactc tctgttcaac accgatgagt
    18421 ggcgggaggc gattgcgctt agcggggcgg atcgacgcca gttcctgctg gagctcttcg
    18481 agcgccagct cggtcagatg gcaagcgtcg agcatgtccg gtcctttcag cttcgcaccc
    18541 aagacggcaa cgactaccgc ttggtcttcg ggctcggcca caccaagggg ctcgaactgg
    18601 ccaaggacgc gatgtggggc gtcgaccccg taggtgggac ctcgtacacc gcaacgactg
    18661 aggctggcca agaggtcctg ttcgctcccg acgacctggt cgatacggcc ccgttgctac
    18721 gtgagctacg cgcgaagttc ggcgatgcct ggttcacgat cgagcaggcc gaggaatgca
    18781 ctctgatcga cacgcccttt cgcaaggggc atttacgcca gcgcacgctt cagccggcag
    18841 agcgcgaggg ggtcatcgag gtgcagcggc caggatcacg cgggtttcgg gacgccaagc
    18901 tgcgcttcgt agactgacgg gcaccccggc gcgccaacat tcggcaacgc ccgcgatgtc
    18961 cgaaccccat gcgaacatgc gttcgtagag aagagggggt cagcaaccgc cagcgaacga
    19021 ggatgcatgg ccgacaacag cgcaatcgag tggaccgagg cgacgtggaa tccggtgacc
    19081 ggctgctcgc aggtctcgcc cggatgcgcc cactgctacg caaagacttt tgctgagcgc
    19141 tggcgaggag tgcctgggca cccctacgag caaggcttcg acctccggct ttggccgcat
    19201 cgccttgagc agccgcttcg gtggacaagg ccgcggatga tcttcgtgaa ctccatgagc
    19261 gacctgtttc atgagggcat ccccctggac tacatcgagc aggtcttcga gatcatgggc
    19321 caagctgagc accacacgtt ccagatcctc accaagcgac acgagcggtt ggccgaacta
    19381 gcgcctcaac tgccatggca tcccaacatc tggattggtg tctcgatcga gaaccgccgc
    19441 tttgtggatc gcgccgacta cttgcgagag gtcaacgcag ccgtcaggtt catatcggcg
    19501 gagcccttgc tagggccact aacaggcctg gatctcagcg gaatcgactg gctcattgct
    19561 ggcggggagt ccggccccaa gcaccggccc gtaagggtcg agtggctacg cgacctgcgc
    19621 gatcagtgcg tggatgaaga ggtcgccttc ttcttcaagc aatggggtgg tcatcgaccg
    19681 aagtcgggcg ggcgagcgct cgacggctgc acgtgggacg aaatgcctgt cgctcgcgtt
    19741 gcgcagttga cctagcgagt agttcgcctg caggtggtcg cagcgcgttc agtcggcagg
    19801 atggcccacc gacttgacag gtgggccaag acgcgcacaa aacgcctgga acgatcagca
    19861 ggagcgcttt cagggcgctc cactagaacc tgctcatttg ctgggcttcc cgcgtctctt
    19921 cctacgtttc cggccctgcc ttacacaaag caggaggtca ctggttcgat cccagtaccg
    19981 cccatgtgga ccgtagactg ggaacgtggc tctcgtagga atgcaccaag ccaggacgca
    20041 tctctcccag ctggtggagc gtgcgttgga gggcgaggag atcgtcttga cgaggcgcgg
    20101 tgagccggta atccgactcg tgcccgagca gccgggcggc ggcttcgcgg ccctggcggg
    20161 tgtgtggcgc gaccgagtgc gcatcgcgga cgacttcgat gggctttcgg aggatctcgc
    20221 cgaaccgctc ggcatggatt cgtgatcacc gtccgccacg ccgatttggt ctatggggtc
    20281 gaggtcgtgt ggtgagggcg ctgtaggttc tcggcatggc cacgttcagg ggatttccga
    20341 gcgaggcgtt ggggttcctg gcggagctgg aggcgaacaa cgaccgggac tggttcaagg
    20401 cgaaccgggg tcgctacgac gaggccctgg tcgcgccggc gacggcgctc ggggaggatc
    20461 tggcggacct gggtcggccg aagctgttcc ggccgtggaa cgacacgcgc ttccatgcgg
    20521 cgccgccgat caaggagcac gtcgggctgg cgatcggcta cgagggggcc ggcgggttct
    20581 acgtcgagct gtcgctcgac gggctgctgg tggccgccgg gctgcacaac ccggcgtcgg
    20641 accaggtcga gcggatgcgg cgcgcgatcg acggggcgcg gaccgccggc gcgctgggca
    20701 aggcgatccg gcacgccgag gccgccgggc tggagctcaa cgggccagac ctcgtccgcg
    20761 gtccgcgcgg ctatccagcc gaccacccgc ggctcgacct gctgcggcgg cgccggctgg
    20821 tcgtctccaa gcggcagcgg acggggcggt ggctggagcg gccggcggcc ggcaagcgaa
    20881 tccgcgagca gctcgaggcg gccgcgccgc tggtgcgctg gctgcgcacc cacgtcgggc
    20941 cctcggagcg cggcgcggcg cgagccggct gagccccggc gatgcgggtt ctttccggac
    21001 tgctgttctt cccgcgcggg gggtcggccc aggtggcgcg ggcgctggcc accgagctgc
    21061 cgcggcaggg atgggacgtc agcgttctct cggggtcgct cgggtccggc cgggccgacg
    21121 cgcgccagtt ctactcaggg ctcgacgtgc actccgtcga cttcgacgcc ggcgacgctc
    21181 cgatgcaccc gtcctacgag gaccggccgg acgcgccgga tcgctgcttc gcgatggtcg
    21241 acgacgaggc ctaccgcgag cacgtcgccg cctgggctcg tgcgatggag cgtgcgggcg
    21301 cggcctcggc cgacgtgctc cacctccacc acctgacgcc gctgaacgag gccgccgcgc
    21361 tcgccgctcc cggcgtgccg atcgtcgggc acctgcacgg gaccgagctg atgatgctcg
    21421 agcggatcgc cgccgggccg ccgccggagt ggacgcacgc cgacgcctgg gcgaagcgga
    21481 tgcgtcgctg ggctcagggc tgcgagcggc tgatcctgct ggccgacagt
//
LOCUS       DATABV010000032         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2660915, whole genome shotgun sequence.
ACCESSION   DATABV010000032 DATABV010000000
VERSION     DATABV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2660915"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..649
                     /locus_tag="VHF88_01515"
     CDS             212..649
                     /locus_tag="VHF88_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160480.1"
                     /translation="MTFPTIEFAAFFTVVLFLSWLLMPHPRAWRPFILVASYLFYGWL
                     DWRWVLLLVGSTVVNTVLANAVARADGAAARKQWVVIAVALNLALLGTFKYLGFFVGS
                     VDDALSGIGLGGLPVLQIVLPIGISFFTFQSISYVVDVYRGDQ"
     gene            653..1609
                     /locus_tag="VHF88_01520"
     CDS             653..1609
                     /locus_tag="VHF88_01520"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX2160481.1"
                     /translation="MSLVDFAVLQAFFPHIVAGPIVRVGEFVPQLRTPRDPRRVLAGP
                     GLFLIAGGLIKKTVVADELARRVVDPVFADPGAHSGLETLLAIYGFAGQIYCDFSGYT
                     DMAIGLALLLGFRLPQNFDRPYLATSLRLFWHGWHMTLSRWLRDYLYIPLGGNRRGPR
                     RTYVNLMLTMLLGGLWHGAAWTFLIWGGIHGGALAGERWVAERYPRLRLPAWAAWLVT
                     FNIVCLAWVFFRAPDLGTAFGVLGQVGAGGPSPLVTPVLVALAVGAVAIQAVPRDFWR
                     RAEQWFVARPAVGQGVALGALVVIADAAVGQQGVAPFIYFQF"
     gene            1611..2747
                     /locus_tag="VHF88_01525"
     CDS             1611..2747
                     /locus_tag="VHF88_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX2160482.1"
                     /translation="MDEPRDRHIDEREHRREQLSDHQPIGGQPNVHDPSHPGIGDPAD
                     PRLTMPAGRVFLIVLVALLTAAIFNSEAIVRAGEGMKPGTTRDVVLSVGRPLEAVTGF
                     VGLHLPRKGFDMAFGQESKTASGTELETGSAAILRRKARREAKPVWRQPTPARPLEVL
                     VTGDSEADLVGLRMADLDTDDLLDVETVARNGTALTNPGFFNWELNAEQEMTSRDPDA
                     VVMLIGANDGFNVDVGGELFAPGTPEWETEFARRVAVVMEALTEKGERPVYWVPPPTA
                     RDETYNEIYRSQNRAVKRAAESVDGARYVDIFSTINDGEYSDQIEIDGRRVLGRQSDG
                     IHFNREGAEVPARLVLQAMAEDYPALADGIPAQEQPNGRTTLGD"
     gene            2754..2939
                     /locus_tag="VHF88_01530"
     CDS             2754..2939
                     /locus_tag="VHF88_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160483.1"
                     /translation="MRNGLCDSCRHQRVVRNTRGSVFSRCERSKTDARYPKYPRVPVL
                     ECPGYEPRDGAEARGAG"
     gene            complement(2923..3546)
                     /locus_tag="VHF88_01535"
     CDS             complement(2923..3546)
                     /locus_tag="VHF88_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160484.1"
                     /translation="MTSRRGHALLVGLCGLLAAGAIGCGGERQDANEDEASYEVAVVD
                     ASFPERLRLAQKSELEITVRNDSGETVPNVAVVLKGLGRASKNSNLQDSDRPVFMIDG
                     EHKEIGGYKEIKLAAPNGGQTALVDTWALGKLAPGAQKTFRWRLTAIQPGPFELSYAV
                     AAGLYGKAKAVGPGDAQPQGSFKGTISSQAPPSRIAADGRTVITPLP"
BASE COUNT          541 a         1369 c         1288 g          604 t
ORIGIN      
        1 gagggcggct accagcaggg caagcgcgcc ggtcctcagg aaattcgatc tcatcgcgtg
       61 aggctctcct tgggccggcg gcttgcaacg ccgccggatc gggggaatcg gacctcatca
      121 acctaagatc gccctcgcca ggggtcttta tccagatggc gaagattccg aagccggcgt
      181 tctcccgctt gtccgagccg accaacccca cgtgaccttc ccgacaatcg agttcgcggc
      241 cttcttcacg gtcgtgctgt tcctgtcgtg gctgctgatg ccgcatccgc gcgcgtggcg
      301 gccgttcatc ctcgttgcgt cctatctctt ctacggctgg ctggactggc gttgggtgct
      361 gctgctggtc ggctcgacgg tcgtcaacac ggtgctggcc aacgcggtcg cacgcgccga
      421 tggcgccgcg gcgcgcaagc agtgggtcgt gatcgcggtc gcgctcaacc tcgcgctgct
      481 cgggaccttc aagtacctcg gcttcttcgt cggctcggtc gacgacgcgc tgagcggcat
      541 cgggctcggc gggctgccgg tgctgcagat cgtgctgccg atcgggatct cgttcttcac
      601 cttccagtcg atctcctacg tcgtcgacgt gtatcgcggc gaccagtgac cggtgtcgct
      661 ggtcgacttc gccgtcctgc aggcgttctt tccgcacatc gtcgcgggac cgatagtccg
      721 cgtcggcgag ttcgtccccc agctgcgaac gccgcgcgac ccgcggcgcg tgctcgccgg
      781 cccgggactg ttcctgatcg ccggcggcct gatcaagaag accgtcgtcg ccgacgagct
      841 cgcgcgtcgg gtggtcgacc ccgtcttcgc cgacccgggc gctcactccg ggctcgagac
      901 gctgctcgcg atctacggct ttgccggcca gatctactgc gacttcagcg gctacaccga
      961 tatggcgatc ggcctggcgc tgctgctcgg cttccgcctg ccgcagaact tcgaccgccc
     1021 gtacctcgcg accagcctca ggctgttctg gcacggctgg cacatgacac tgtcgcgctg
     1081 gctgcgcgac tacctctaca tcccgctcgg cggcaaccgc cgcggaccgc ggcgaaccta
     1141 cgtcaacctg atgctgacga tgctgctcgg cggcctctgg cacggcgccg cctggacgtt
     1201 cctgatctgg ggcggcatcc acggcggcgc gctggccggc gagcgctggg tcgccgagcg
     1261 ctacccgcgc ctgaggctgc cggcatgggc ggcctggctg gtcaccttca acatcgtctg
     1321 cctcgcgtgg gtcttcttcc gcgctcccga cctcggcaca gcgttcggcg tgctcgggca
     1381 ggtcggtgcg ggcggcccct cgccgttggt cacgccggtg ctcgtcgcgc tggccgtcgg
     1441 cgcggttgcg atccaggcgg tcccgcgcga cttctggcgc cgcgccgagc agtggttcgt
     1501 cgcgcggccg gcggtcggcc agggagtcgc gctcggcgcc ctcgtcgtga tcgccgacgc
     1561 cgcggtcgga cagcagggcg ttgccccctt catctacttc cagttctgac atggacgagc
     1621 cacgcgacag gcacatcgac gagcgcgagc accgccgcga gcagctgagc gaccaccagc
     1681 cgatcggcgg tcagccaaac gtccacgacc cctcgcaccc cgggatcggc gacccggccg
     1741 acccgcgact gacgatgccg gcgggccgcg tcttcctgat cgtcctcgtc gcgctgctga
     1801 cagcggcgat cttcaactcc gaggcgatcg tccgcgccgg cgaggggatg aagccgggga
     1861 ccaccaggga cgtcgtcctg tcggtcggcc gcccgctcga ggcggtcacc ggcttcgtcg
     1921 gcctgcacct gccgcgcaag ggcttcgaca tggccttcgg ccaggagagc aagaccgcgt
     1981 cggggaccga gctcgagacc ggctcggcag cgatcctgag acgcaaggcg aggcgcgagg
     2041 ccaagcccgt ctggcgccag ccgacgcctg cccgaccgct cgaggtgctc gtcaccggcg
     2101 actccgaggc cgacctcgtc ggcctgcgga tggccgacct cgacaccgac gacctgctcg
     2161 acgtcgagac ggtcgctcgc aacggcaccg cgctgaccaa cccgggcttc ttcaactggg
     2221 agctgaacgc cgagcaggag atgaccagcc gcgacccgga cgccgtcgtg atgctgatcg
     2281 gcgccaacga tggcttcaac gtcgacgtcg gcggcgagct gttcgcaccg gggaccccgg
     2341 agtgggagac cgagttcgcc cgccgcgtcg ccgtcgtgat ggaggccctg accgagaaag
     2401 gcgagcggcc cgtctactgg gtcccgcccc cgaccgcccg cgacgagacc tacaacgaga
     2461 tctaccgctc ccaaaaccgc gcggtgaagc gcgccgccga gtcggtcgac ggcgcccgct
     2521 acgtcgacat cttctccacg atcaacgacg gcgagtactc cgaccagatc gagatcgacg
     2581 gccgccgcgt cctcggccgc cagtccgacg gcatccactt caaccgcgag ggcgccgagg
     2641 tcccagcccg gctcgtcctc caagcgatgg ccgaggacta cccggcccta gccgacggaa
     2701 tacccgccca ggagcagccc aacgggcgca cgaccctcgg cgactaggga cgcatgcgca
     2761 acggcctctg cgacagctgc cgccaccagc gcgtcgtgcg caacacgcgc ggcagcgtct
     2821 tcagccgctg cgagcggtcg aagaccgacg cgcgctatcc gaagtacccg cgcgtgccgg
     2881 tgctcgagtg cccgggctac gagccgcgcg acggcgcgga ggctagggga gcggggtgat
     2941 cacggtgcgg ccgtcggcgg cgatccggct aggcggggcc tgcgagctga tcgtgccctt
     3001 gaacgagccc tgcggctggg cgtcgcccgg accgaccgcc ttggccttgc cgtagagacc
     3061 ggcggcgacc gcgtagctga gctcgaaggg gcccggctgg atcgccgtca gccgccagcg
     3121 gaaggtcttc tgcgcgccag gggcgagctt gccgagcgcc catgtgtcga cgagcgcggt
     3181 ctggccgccg ttcggcgcgg cgagcttgat ctccttgtag ccgccgatct ccttgtgctc
     3241 gccgtcgatc atgaacaccg ggcggtcgga gtcctggagg ttggagttct tcgaggcgcg
     3301 gccgaggccc ttgaggacca cggcgacgtt tgggaccgtc tcgccggagt cgttgcggac
     3361 ggtgatctcg agctcggact tctgggcgag gcgcagacgc tccgggaagc tcgcatcgac
     3421 gaccgcgacc tcgtaggagg cctcgtcctc gttggcgtcc tgacgctccc ctccgcagcc
     3481 gatcgccccg gcggccagca ggccgcacaa accgaccagc agggcatggc ctctcctcga
     3541 cgtcaaagcg agcgcaacat agcacggggc ctcggccgcc cgacgcactt ccgctgtgcc
     3601 ggatggccgc tcggttcctg gttggcaccc ccggatggca tggcgtagtg ttcgggatcg
     3661 acggagactc gcggcgcacg tgccgggagc ttcgacacgg aggagcatca gtggttcgcg
     3721 ccgcagccga tcgagcattc ctgcccacca agaacctgct ggagcaggtc ggcgacatga
     3781 tgatcctgac cggcaagacg ct
//
LOCUS       DATABV010000033         8831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2741454, whole genome shotgun sequence.
ACCESSION   DATABV010000033 DATABV010000000
VERSION     DATABV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741454"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1303
                     /locus_tag="VHF88_01540"
     CDS             74..1303
                     /locus_tag="VHF88_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX2160485.1"
                     /translation="MSTGALSDVKVLDLSRLLPGGFCSLLLADFGAEVLKIEDTGMGD
                     YIRWAPPYYEGADDSAKSALYLSLNRNKRSIRIDLKDERGREVLLKLVRDYDVVLESF
                     RPGVLDRLGVGYERLREENPGLVYCAISGYGQDGPYRDRAGHDMNYLGLVGLLGLTGE
                     KGGPPVQAAGQIADLGGGALMAAFGIMAALRERERSGEGQLVDVSMADGALSWLAMVA
                     GKYFADGVAPQRGDLELAGRYICYRPYACSDGDVTLGALEPKFWQAWCRGVGREDLIE
                     KQFESPGSAAHGEVERIFAARSRAQWEAFAAEHDCCLEPVLGLDEALGSELVRARQMV
                     VELDQPGAERPVRQLGVPIKLSRTPGGVRAPGPALGEHTREVLSDLGYSDGEIADLER
                     AGAVAGPTAGAAGTFMA"
     gene            1300..2034
                     /locus_tag="VHF88_01545"
     CDS             1300..2034
                     /locus_tag="VHF88_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2160486.1"
                     /translation="MSSAHDAEPSGANGADGLLKMSELAEASGVSAGTIKHYLREGLL
                     PEPVKTSRNMAYYPPAFVERIRLIKRLQEERFMPLKAIRRVLDDDPERARALVEVEDR
                     ILERAQRGDGERLSRAAAERRFGIPREALERLEKIGVLTPNSRGYGALDARMLEAISR
                     FRAGGYDEQIGFTVYDTLRYKSALEQLVKEEVEVVLERLAGDVSPERIVELLEAGAEP
                     LRDLIAAMHSKLLVEELRRQRDSRAG"
     gene            complement(2088..2951)
                     /locus_tag="VHF88_01550"
     CDS             complement(2088..2951)
                     /locus_tag="VHF88_01550"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HEX2160487.1"
                     /translation="MATLPLRGAEVRDLGLVLPPARMPAWRAGRPLKRWRYVGVYTPE
                     LMLCVADARVGPVRQRWWAVTLPSGELRERTSFGAGGVTIAGSTVRVDAADVRIDLVL
                     DESAGVEVVSPVNDRGAYIWTRKQACVPVSGTVAIDGVRHAIDGDAGFIDDSAGYHER
                     HTVWDWSAGVGTAVDGRRVGWNLVTGLHDDPRASERTVWVDGEPQAVGLVAFAKDLSE
                     VSSAPGLDTPADNGLALRFSEWSSREHSTNALLLRSRYRQPFGSFSGSLPGGLQLREG
                     YGVMEHHDVHW"
     gene            3019..4497
                     /locus_tag="VHF88_01555"
     CDS             3019..4497
                     /locus_tag="VHF88_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2160488.1"
                     /translation="MEAATAPEVDEPVSTSSQRPPPIHESALRRMVRDVRDELRTRDP
                     WPPGAADFDVGRTRQMVGDPLPILLECYERYGPVFSLRILHARQVWALGPEANHQILV
                     GDARNFRWREGSFGDLIPLLGDGLLTIDGGYHRRARRIMLPAFHRERIAASAETMVAE
                     AEAALAGWRPGDVVDVYHWARNLAMRVAMRALLGLDPDDGDTGARAAVEFERALSFYG
                     TDFAVRVLRGPGSPWSRMMASRAELDRILYSEIRRRRTSGAHGDGDVLGMLLAATDEQ
                     GEPLSDQEVRDQAITLLFAGHDTTTSTVSFLLYELARNPRELAALYAEQDDLLPGGRA
                     PELEHLAGGLPRLEMTLDETLRLYPPAWIGPRRAVAACEIAGVHVPANAYVNYCSWAS
                     HRLPDVFSDPETFVPERFTPERKAALPKGAYVPFGGGSRTCIGMRFGQMEIRAIATLL
                     LQRFRLELQPGRTMTVRQMPTLSPRDGLTMVVRERGLADAVA"
     gene            4554..5384
                     /locus_tag="VHF88_01560"
     CDS             4554..5384
                     /locus_tag="VHF88_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX2160489.1"
                     /translation="MRAAAIQMNSTADKQRNLATAERLVRAAAADGSTLVVLPEKFNV
                     LGTPDQLVEGAEPLDGPTLAWARALAQELSIDLVAGSIPERRAGRDKLANTSVHIGPD
                     GVDRAAYRKIHMFDVTVGGREYRESATDEAGDEIVLSDSDGVSLGLTVCYDLRFPELF
                     RILAVRGARIFSVPSAFTAVTGAAHWDTLVRARAIENQAFVVAADQVGVHPGGNESYG
                     GSQIVDPWGDVLARADDERECFVAADVDLARQDEIRATLPSLANRVAAAYRWPQQAGA
                     "
     gene            5384..6022
                     /locus_tag="VHF88_01565"
     CDS             5384..6022
                     /locus_tag="VHF88_01565"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2160490.1"
                     /translation="MAASSRPGAVDKRRLILDAAITVFARQGFHHCRVSDVADEAGVA
                     YGLVYHYFDSKEQMLNELFLERWQIMLDAIGEIDARDELAPREKLHEVAGFIIDSYRH
                     EPDLMKVIIVEVTRAANSFGRLHLEKISEAYGGIAEIVESARRDGTFKQDISAQFAAM
                     CFYGAIEQLLSAWIFDLLPQTEEEFERAKTLVVEAICGGLEVSPSGAPAVSG"
     gene            6034..6171
                     /locus_tag="VHF88_01570"
     CDS             6034..6171
                     /locus_tag="VHF88_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160491.1"
                     /translation="MQNDLVKRLMWSGVLAGISALSTIVAHRIAAYVWLRVFDEDPPD
                     A"
     gene            6171..6383
                     /locus_tag="VHF88_01575"
     CDS             6171..6383
                     /locus_tag="VHF88_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160492.1"
                     /translation="MESSAQGAGAAGDEGTREIPPVGATAAGPSPVEAQASASGDPFA
                     ERPELYVGAAFAGGLVLAKILGRLGR"
     gene            6380..6847
                     /locus_tag="VHF88_01580"
     CDS             6380..6847
                     /locus_tag="VHF88_01580"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2160493.1"
                     /translation="MSGQGNADKTLGEIVSEVSEKASLLVREEIELAKAEVVAKAKKM
                     GRGAGIGVAAGVFLIFALVMLLHTLAWLLVELFDFQIWVGFGIVTLVLVLLGAIAGAL
                     AAKWLKAGPPTPDMAIEQAKITRETLEAQTAQRDQLERSLEGAKETSEETAKR"
     gene            6844..7080
                     /locus_tag="VHF88_01585"
     CDS             6844..7080
                     /locus_tag="VHF88_01585"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX2160494.1"
                     /translation="MTSVPANRSPEQIRASIEETRRELAFSVNDLRSKVTELADWRRQ
                     LVENRNTALTTAAVTGFVIGGGIAATVSLFRRRR"
     gene            complement(7081..8181)
                     /locus_tag="VHF88_01590"
     CDS             complement(7081..8181)
                     /locus_tag="VHF88_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2160495.1"
                     /translation="MDRQVPAARAVARTVLIVVAVLLVLYLIYQLRKPIAWLLIATFL
                     AVALSGPVNVLHRHMRRGFAIAIVYLGLLMIPIGIGALIVPPIVTGAEDLAQNAPAYA
                     NDLTEFVNENDRLQELNDDYDITAKLQDEAAKLPEKLGDAASVLSDVGLGIVNSIFAV
                     VTILILTAFMLGGGRRWIATWLRFAPPDRAERIERVVDRSARAVGNYVAGALAQALLA
                     AVTSFVVLKILGIPFAAPLALIIFFLDLIPMVGATLGAIIVGVVTLFGDFPMDTVIWA
                     VWSIVYQQVENNLVQPQIQRRAVDINPFLVIVSVLFGGTLLGVLGALMAVPVAATIQI
                     ALREWADLQGIRPRADAPPPEPPPAEPPPAPA"
     gene            complement(8225..>8831)
                     /locus_tag="VHF88_01595"
     CDS             complement(8225..>8831)
                     /locus_tag="VHF88_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2160496.1"
                     /translation="HISGGISGTVESARQAAEQLERDGPGGERVRIVDSATAAGGQGI
                     VTLAAARCAQLGGTLDEVVDAADRARAELKMWFAINTLEFLKRGGRIGAASAWIGSTL
                     KIKPILTVERELSPVERVRTSSRMFERLVDYAQQRHDSGADAWVVQHIAAPDTAQRLA
                     ERCHEVFGREPLFISEVGPVLSAHTGPGMLGVGSVPAHFYD"
BASE COUNT         1316 a         3136 c         3128 g         1251 t
ORIGIN      
        1 gccgtcgcgc cgcgacggtc gacgaggcgc ggcagctgct cggcctgagg gcacgggctt
       61 ccgcgtaggc gcagtgagta ccggggcgct cagcgacgta aaggtcctcg acctgagccg
      121 gctgctgccg ggcggcttct gctcgctgct gctggccgac ttcggcgccg aggtgctgaa
      181 aatcgaggac accggcatgg gcgactacat ccgctgggcg ccgccctact acgagggcgc
      241 agacgactcg gcgaagtcgg cgctctacct gtcgctgaat cgcaacaagc gctcgatccg
      301 gatcgacctc aaggacgagc gtgggaggga ggtgctgctg aagctcgtgc gcgactacga
      361 cgttgtgctc gagtccttcc gccccggtgt tctcgaccgg ctcggcgtcg gttatgagcg
      421 gctgcgcgag gagaaccccg gcctcgtcta ctgcgcgatc agtggttacg gccaggacgg
      481 cccctaccgc gaccgcgcgg gccacgacat gaactacctc ggcctcgtcg gcctgctcgg
      541 gctgaccggc gagaagggcg gtccgccggt ccaggcggcg ggccagatcg ccgacctcgg
      601 cggtggcgcg ttgatggccg ccttcggaat catggccgcc ctgcgcgagc gcgagcggtc
      661 gggggagggg cagctggtcg acgtctcgat ggccgacggc gcgctctcct ggctggcgat
      721 ggtggccggc aagtacttcg ccgacggcgt cgcgccacag cgtggcgacc tcgagctcgc
      781 cggcaggtac atctgctatc gcccctatgc gtgcagcgac ggcgacgtga cgctcggcgc
      841 gctcgagccg aagttctggc aggcgtggtg tcgcggcgtc gggcgcgagg acctgatcga
      901 gaagcagttc gagtcgccgg gctccgcggc ccacggcgag gtcgagcgga tcttcgccgc
      961 gcgcagccgc gcgcagtggg aggcgttcgc cgccgagcac gactgctgcc tcgagccggt
     1021 gctcggcctc gacgaggcgc tcggctccga gctcgtgcgc gcacggcaga tggtcgtcga
     1081 gctcgaccag ccgggggccg agcgaccggt gcgccagctc ggcgtgccga tcaagctgtc
     1141 gcgcacgccg ggcggcgttc gggcgcccgg accggcgctc ggcgagcaca cgcgcgaggt
     1201 gctgagcgac ctcgggtact ccgacggcga gatcgccgac ctcgagcggg cgggagcggt
     1261 cgcgggcccg acggccggcg ccgccgggac gttcatggca tgagctccgc gcacgacgcc
     1321 gagccgagtg gcgccaacgg cgccgacggc ctgctgaaga tgagcgagct ggcggaggcc
     1381 agcggcgtca gcgccggcac aatcaagcac tacctgcgcg agggcctgct accggagccg
     1441 gtcaagacct cacgcaacat ggcctactac ccgccagcgt tcgtcgaacg gatccgcctg
     1501 atcaagcgcc tccaggagga gcgcttcatg ccgctcaagg cgatccggcg ggtgctcgac
     1561 gacgaccccg agcgcgcccg cgcgctcgtt gaggtcgagg accggatcct cgagcgggcg
     1621 cagcgcggcg acggcgagcg gctctcgcgc gccgcggccg agcgccgctt cggcatccca
     1681 cgcgaggcgc tcgagcggct cgagaagatc ggcgtgctga cgccgaacag ccgtggctac
     1741 ggagcgctcg acgcgcgcat gctcgaggcg atcagccgct tccgcgccgg tggctacgac
     1801 gagcagatcg gcttcaccgt ctacgacacc ctgcgctaca agagcgcgct cgagcagctc
     1861 gtcaaggagg aggtcgaggt cgtcctcgag cgcctcgccg gcgacgtcag tcccgagcgc
     1921 atcgtcgagc tgctcgaggc cggcgcggag ccgttgcgcg acctgatcgc cgccatgcac
     1981 tcgaagctgc tcgtcgagga gctccgccgc cagcgcgact cgcgcgccgg ctgaggcacc
     2041 gagcggagga ttgtgcggcc tataggccac acaaatcctt cactcggtca ccaatggacg
     2101 tcgtggtgct ccatgacgcc atagccctcg cggagctgaa ggccccccgg aaggcttccg
     2161 ctgaagctgc cgaagggctg gcgatagcgc gagcgcagca gtagcgcgtt ggtcgaatgc
     2221 tcgcgcgatg accattcgct gaagcggagc gctaagccgt tgtctgctgg cgtgtcgagc
     2281 cccggcgctg acgagacctc cgacagatcc ttggcgaagg ccaccagccc caccgcctgc
     2341 ggctcgccgt cgacccagac cgtccgctcg ctggcacggg gatcgtcgtg gaggccggtg
     2401 acgaggttcc agccgacccg gcgcccgtcg acggccgtgc cgacgcccgc cgaccagtcc
     2461 cagacggtgt ggcgctcgtg gtagccggcc gagtcgtcga tgaagccggc gtcgccgtcg
     2521 atggcgtggc ggacgccgtc gatcgcgacc gttccgctga ccggcacaca cgcctgcttg
     2581 cgcgtccaga tgtaggcgcc gcggtcgttc accggcgaga cgacctcgac gccggcggac
     2641 tcgtcgagca cgagatcgat ccgcacgtcg gcggcgtcaa cacgcaccgt cgagccggcg
     2701 atcgtcacgc cgccggctcc gaagctggtc cgctcgcgca gctcgccgct cgggagggtg
     2761 accgcccacc aacgctgccg caccggcccg acgcgcgcgt cggcgacgca cagcatcagc
     2821 tccggcgtgt agacgccaac gtaacgccag cgcttgagcg gccggccggc gcgccatgcc
     2881 ggcatccgcg ccggcggcag caccaggccg aggtcgcgta cctcggcgcc gcggagcgga
     2941 agcgtggcca tccagccacc atattcagtc ttggctggct ggccaacata gaagctcgac
     3001 gagctatgct cgcctgcgat ggaggcagcg accgcgcccg aggtagacga gcccgtctcg
     3061 acctcgtcgc agcggccacc gccgatccac gagagcgcgc tgcgccggat ggtccgcgac
     3121 gtccgcgacg agctgcgcac ccgcgacccc tggccgcccg gcgccgccga cttcgacgtc
     3181 ggccgcacgc ggcagatggt cggcgatccg ctgccgatcc tgctcgagtg ctacgagcgc
     3241 tatgggccgg tgttctcgct gcggatcctc cacgcgcgcc aggtctgggc gctcggcccg
     3301 gaggccaacc accagatcct ggtcggcgac gcgcgcaact tccgctggcg cgagggcagc
     3361 ttcggtgact tgatcccgct gctcggcgac ggcctgctga cgatcgacgg cggctaccac
     3421 cgacgcgcgc ggcgcatcat gctgcccgcc tttcaccgcg agcggatcgc cgcgtcggcc
     3481 gagacgatgg tcgccgaggc cgaggcggcg ctggccggct ggcggccggg cgacgtcgtc
     3541 gacgtctacc actgggcgcg caacctcgcg atgcgggtgg cgatgcgcgc gctgctcggg
     3601 ctggacccgg acgacggcga caccggcgcg cgcgccgcgg tcgagttcga gcgggccctc
     3661 tccttctacg ggaccgactt cgcggtgcgc gtgctgcgcg gtccgggctc gccgtggagc
     3721 cggatgatgg cgtcgcgcgc ggagctcgac cggatcctct actccgagat ccgccgtcgc
     3781 cgcacgagcg gcgcccacgg tgacggcgac gtgctgggga tgctgctcgc ggcgaccgac
     3841 gagcagggcg agccgctctc cgaccaggag gtgcgcgacc aggcgatcac gctgctgttc
     3901 gccggacacg acacgacgac ctcgacggtc tcgttcctgc tctacgagct ggcccgcaac
     3961 ccgcgcgagc tggcggcgct ctacgccgag caggacgacc tgctgccggg cggtcgggcg
     4021 cccgagctgg agcacctcgc cggcgggctg ccgcggctcg agatgacgct cgacgagacg
     4081 ctgcggctct acccgcccgc ttggatcggt ccccgccgcg cggtcgcggc ctgcgaaata
     4141 gcgggcgtcc acgtcccggc gaacgcctac gtcaactact gctcgtgggc gagccaccgg
     4201 cttcccgacg tcttctcgga tccggagaca ttcgtccccg agcgctttac gccggagcgc
     4261 aaggcggcgc tgccgaaggg cgcctatgtc ccgttcggcg gcggctcgcg cacctgcatc
     4321 gggatgcgct tcggccagat ggagatccgg gcgatcgcga cgctgctgct gcagcgcttc
     4381 cgtctcgagc tgcagcccgg tcgcacgatg accgttcgcc agatgccgac cctgagcccc
     4441 cgcgacggcc tgacgatggt ggtccgcgag cgcggtctcg ccgatgctgt cgcgtgaccg
     4501 ccgccagcgt tagactcgtt gactgacgag tcaatctggc tcagggagcg acggtgcgag
     4561 ccgcggctat tcagatgaac tccactgcgg acaagcagcg caacttggcg accgccgagc
     4621 ggctcgtccg cgcagccgcc gccgacggct cgacgctggt cgtcctgccc gagaagttca
     4681 acgtgctcgg cacgcccgac cagctcgtcg agggggccga gccgctcgac ggcccgacgc
     4741 tcgcctgggc gcgcgcgctc gcgcaggagc tgagcatcga cctcgtagcc ggctcgatcc
     4801 ccgagcgccg cgccggccgc gacaagctcg ccaacacctc cgtccacatc gggcccgacg
     4861 gggtcgaccg tgccgcctac cgcaagatcc acatgttcga cgtcacggtc ggcggccgcg
     4921 agtaccgcga gtcggccacc gacgaggcgg gcgacgagat cgttctcagc gactctgatg
     4981 gcgtcagcct cggcctgacc gtctgctacg acctgcgctt tcccgagctg ttccgcattc
     5041 tcgccgtccg tggtgcccgc atcttcagcg tgccgtcggc gttcaccgcc gtcacgggcg
     5101 cggcccactg ggacacgctg gtgcgtgcac gcgcgatcga gaaccaggcg ttcgtcgtgg
     5161 cagccgacca ggtcggagtg catcccggcg gcaacgagag ctacggcggc tcgcagatcg
     5221 tggacccgtg gggcgacgtg ctcgcacggg ccgacgacga gcgggagtgc ttcgtcgccg
     5281 ccgacgtcga cctcgcacgc caggacgaga tccgcgcgac gctgccgagc ctcgccaacc
     5341 gcgtggcggc cgcctaccgc tggccgcagc aggcgggcgc ctgatggccg cctcgtcgcg
     5401 ccccggcgcg gtcgataagc ggcggctgat cctcgacgcg gcgatcaccg tcttcgcgcg
     5461 ccagggcttc caccactgcc gcgtctccga cgtcgccgac gaggcgggcg tcgcctatgg
     5521 cctggtctac cactacttcg actcgaagga gcagatgctc aacgagctgt tcctcgagcg
     5581 ctggcagatc atgctcgacg cgatcggcga gatcgacgcg cgcgacgagc ttgcgccgcg
     5641 cgagaagctg cacgaggtcg ccggcttcat catcgactcc taccgccacg agcccgacct
     5701 gatgaaggtg atcatcgtcg aggtcacgcg cgcggcgaac tcgttcggtc gcctgcacct
     5761 cgagaagatc agcgaggcct acggcgggat cgccgagatc gtcgagtcgg cccgccgcga
     5821 cggcaccttc aagcaggaca tctcggccca gttcgcggcg atgtgcttct acggcgcgat
     5881 cgagcagctg ctgtccgcct ggatcttcga cctgctgcct cagaccgagg aggagttcga
     5941 gcgcgcaaag acgctcgtcg tcgaggcgat ctgcggcggc ctcgaggtct caccctccgg
     6001 tgctccggcg gtatctggtt gaatcgccag acgatgcaga acgatctggt caagcggttg
     6061 atgtggagcg gggtcctcgc ggggatcagc gccctgtcga cgatcgtcgc ccaccggatc
     6121 gccgcctacg tgtggctgcg cgtcttcgac gaggacccgc cggacgcgtg atggagtcct
     6181 ccgcccaggg agccggcgcc gcgggcgacg agggaacccg cgagatcccc cctgtcggag
     6241 cgaccgcagc cggtccctcg ccggtcgagg cgcaggcctc cgcctccggc gacccgttcg
     6301 ccgagcgacc ggagctgtac gtcggggctg ccttcgccgg cgggctcgtg ctcgccaaga
     6361 tcctcgggcg gctgggtcga tgagcggcca gggcaacgcc gataagactc tcggcgagat
     6421 cgtctcagag gtctcggaga aggcatcgct gctcgtccgc gaggagatcg agctcgccaa
     6481 ggcagaggtc gtcgccaagg cgaagaagat ggggcggggg gccgggatcg gcgtcgccgc
     6541 cggcgtcttc ctgatcttcg cgctcgtcat gctcctgcac accctggcgt ggctgctcgt
     6601 cgagctgttc gacttccaga tctgggtcgg gttcgggatc gtcacgctcg tgttggtgct
     6661 gctcggcgcg atcgccggcg cgctcgcggc caagtggctg aaggccggac cgccgacccc
     6721 cgacatggcg atcgagcagg cgaagatcac acgcgagacg ctcgaggcgc agaccgccca
     6781 gcgcgatcaa ctggagcgct cgctcgaggg cgccaaggag acctcggagg agaccgccaa
     6841 gcgatgacca gcgtgcccgc aaaccgcagt cccgagcaga tccgcgcctc gatcgaggag
     6901 acgcgccgcg agctcgcctt ctcggtcaac gacctgcgct ccaaggtgac cgagctcgcc
     6961 gactggcgcc gccagctcgt cgagaaccgc aacactgcgc tgaccacagc agccgtcacc
     7021 ggtttcgtca tcggcggcgg tatcgccgca acggtttccc tgttccgcag gcgtcgctag
     7081 ctacgcgggc gccggcggcg gctctgccgg cggtggctcc ggcggcggtg catccgcgcg
     7141 cggccggatg ccctggaggt cggcccactc gcgcagcgcg atctggatcg tcgccgcgac
     7201 gggcacggcc atcagtgcgc cgaggacgcc gagcagcgtg ccgccgaaga gcaccgacac
     7261 gatcaccagg aaggggttga tgtcgacggc ccgccgctgg atctgcggct ggacgaggtt
     7321 gttctcgacc tgctggtaga cgatcgacca gaccgcccag atgaccgtgt ccatcggaaa
     7381 gtcgccgaac aacgtgacca ccccgacgat gatcgcgccg agcgtcgcgc cgaccatcgg
     7441 gatcaggtcg aggaagaaga tgatcagcgc cagcggcgcg gcgaacggga tgccgaggat
     7501 cttcaggacg acgaacgacg tgaccgcagc cagcagcgcc tgggccagcg cgccggcgac
     7561 gtagttgccg accgcccgcg ctgaccggtc gacgacgcgt tcgatccgct cggcgcggtc
     7621 ggggggcgcg aagcgcagcc aggtcgcgat ccagcggcgc ccgccgccga gcatgaaggc
     7681 ggtcaggatc aggatcgtca cgaccgcgaa gatcgagttg acgatcccga ggccgacgtc
     7741 gctgagcacg ctcgcggcgt cgccgagctt ctccggcagc ttcgccgcct cgtcctgcag
     7801 cttggcggtg atgtcgtagt cgtcgttgag ctcctgcagc cggtcgttct cgttgacgaa
     7861 ctcggtcagg tcgttggcgt aggcgggtgc gttctgcgcc aggtcctcgg cgccggtgac
     7921 gatcggcggc acgatcagcg ctccgatccc gatcgggatc atcagcaggc cgaggtagac
     7981 gatcgcgatc gcgaagccgc gccgcatgtg gcggtgcagc acgttgaccg gtcccgagag
     8041 ggccaccgcc aggaaggtcg cgatcaatag ccaggcgatc ggcttgcgca actggtagat
     8101 caggtaaagc accagcagca ccgcgaccac gatcaggacg gtgcgcgcca ccgcccgcgc
     8161 cgcgggtacc tgacgatcca tgccgcgaag attgccgccc gcctcggacg gctagatcgg
     8221 ccgctcaatc gtagaaatgc gcgggcaccg agccgacgcc gagcatgccc ggccccgtat
     8281 gggcgctcag cacggggccg acctcgctga tgaagagcgg ctcgcggccg aagacctcgt
     8341 gacagcgctc ggcgagccgc tgagcggtgt cgggagcggc gatgtgctgg accacccagg
     8401 cgtcggcgcc cgagtcgtgg cgctgctgcg cgtagtcgac cagccgctcg aacatccgcg
     8461 agctggtgcg cacgcgctcg accgggctca gctcgcgctc gacggtcaag atcggcttga
     8521 tcttcaacgt cgagccgatc caggcgctcg ccgcgccgat ccgcccgccg cgcttgagga
     8581 actcgagcgt gttgatcgcg aaccacatct tgagctccgc ccgtgcgcgg tcggccgcgt
     8641 cgacgacctc gtcgagcgtg ccgccgagct gggcgcagcg cgccgcggcg agcgtgacga
     8701 tcccctggcc gccggcggcg gtcgcggagt cgacgatccg cacccgctcg ccgccggggc
     8761 cgtcgcgttc cagctgctcg gccgcctggc gcgctgactc gacggtgccc gagatgccgc
     8821 ccgagatgtg a
//
LOCUS       DATABV010000034         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1537800, whole genome shotgun sequence.
ACCESSION   DATABV010000034 DATABV010000000
VERSION     DATABV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537800"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..>2988
                     /locus_tag="VHF88_01600"
     CDS             25..>2988
                     /locus_tag="VHF88_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160497.1"
                     /translation="MSTQLQDTQASLNAAGERLEEAGRNILVSLDQHIHEAETILVAA
                     NERLVSSGRNVEGTLRNQIEVVAAELASASERFNNTGAEAARGISAQVERVEAAVASS
                     AQRLGATGDKLVGDIHRQLGAIDAVFHASTEQFKKTGETVSGAVGAQIARIDASVVTA
                     NERLGATGGRVVADINRHIGAVDAAIAAAGDRFGKDGEALSARLDGEVARVSAALAEA
                     RDRFGKTGEDIAGGVGAQIDRIDMAVASAGERFGRTGMEVSIAVDMQAQRIESALAAA
                     AERIDKTGEQISGGISAQVGRVDAAVVSANQRLGATGERILGDITRQVGAVDAALSAA
                     NEHMSTTGETVAAGIAAQVDRVEGALATASQRLGATGESVVGDINAQIRTVDATLDSA
                     RVRFGKTGEELADQLNTQVDRVDIALAAASLRLEASGEKVLENIDERIKAVDTTIGSA
                     SERFGKAGAQVAAGIAEQVDRVEALATSAGERLDGTGERLVGDINRQVGAVDAALAAA
                     GERFRAAGVEVALEVGMQVDRVDSALSGASRSLGATGEKLIGDLDGKIAAIDASLGAA
                     NQRFEASGSAIADGVGTQIERVEAALASAGQRLGTTSDRLVGEINRQIGAVDAALISA
                     NDRFAATGDRLAVSIDERIENVEGRLARANDRLNTTGDRIAGNVIQQLGSVEAALALA
                     DERLGTAGRELAAHVGEQMSAVELSLQLAQARFAESGDHTAERVRTTMESLKDVIATT
                     GDATGTALTQRGEEVRSLIEKNIERVSEVLDNRGGALAATLGERSAALGSLIDTRVAQ
                     IGETLDSRLTGFESRLTESAEAAAIRLASSASTIEIAVEGRLSGFENRVTARIGEVTH
                     NFAQRADGFANTLDDRLERINSTLDTRARQISDTLVARTREMAAAFHDGHNEMSTALD
                     TRLQTLGDHLSTRTQELTDTIATRMMEINQGFGERLLGVQTSFSDRANSLQSALAATG
                     TMLNERTDQLNATLAA"
BASE COUNT          507 a         1035 c         1072 g          374 t
ORIGIN      
        1 agcggccagg ccgtggcgcg cgcgatgagc acgcagctgc aggacaccca ggcttcgctc
       61 aatgccgccg gcgagcggct ggaggaggcc ggccgcaaca tcctggtctc gctcgaccag
      121 cacatccatg aggccgagac gatcctggtg gctgcgaacg agcgcctggt gagctccggc
      181 cgcaacgtgg aagggacgct acgcaaccag atcgaggtcg tcgccgccga gctggcgtcg
      241 gccagcgagc ggttcaacaa caccggcgcc gaagcggcgc gcggcatctc tgcgcaggtg
      301 gagcgcgtcg aggcagctgt cgcgtcgagc gcgcagcggc tcggcgccac gggagacaag
      361 ctggtcggcg acatccaccg ccagctcggc gccatcgacg cggtgttcca tgcctcgacc
      421 gaacagttca agaagaccgg cgagacggtg agcggcgccg ttggcgcgca gatcgcccgc
      481 atcgacgcca gcgtcgtgac cgccaacgag cggctgggcg ccaccggcgg gcgcgtcgtc
      541 gccgacatca atcgccacat cggggccgtc gatgccgcga tcgccgcggc gggagaccgg
      601 ttcggcaagg acggcgaggc attgtcggcc cggctcgacg gcgaggtggc gcgggtctcg
      661 gccgcgctgg ccgaggcgcg cgatcgcttc ggcaagacgg gtgaagacat cgccggcggc
      721 gtgggcgcgc agatcgaccg gatcgatatg gcggtcgcct cggcaggcga gcgattcggc
      781 aggaccggca tggaggtctc tattgccgtc gacatgcagg cgcagcggat cgagtccgcc
      841 ctcgccgcgg cggcggagcg gatcgacaag accggcgaac agatttcagg cggcatctcg
      901 gcgcaggtcg gccgcgtcga tgccgcggtg gtctcggcca atcagcggct cggggccact
      961 ggcgagcgca ttctgggcga cattacgcgg caggtcggcg cggtcgacgc ggcgctttct
     1021 gccgccaacg agcatatgag cacgacgggc gagaccgttg cggccggcat cgccgcgcaa
     1081 gtcgaccggg tggaaggtgc gttggcgacg gcgagccagc gtctcggcgc cacgggtgag
     1141 agtgtggtcg gcgacatcaa cgcgcagatc cgcacggtgg acgcgaccct cgactccgcc
     1201 cgggtgcggt tcggcaagac cggcgaggag ctcgcggatc agctcaacac gcaggttgac
     1261 cgcgtcgaca tcgccctcgc cgcggcgagc ctgcggctgg aggccagcgg cgagaaggtg
     1321 ctcgaaaata tcgacgagcg catcaaggcc gtggatacga ccatcggctc cgccagcgag
     1381 cgcttcggaa aggctggtgc ccaggtggcg gccggcatcg ccgagcaggt ggaccgcgtc
     1441 gaggcgctcg ccacctcggc cggcgagcgg cttgacggca ccggcgagcg gctcgtcggc
     1501 gacatcaacc ggcaggtcgg agccgtggac gcggcgttgg ccgccgccgg cgagcggttc
     1561 cgtgccgccg gcgtcgaggt cgccctggaa gtggggatgc aggtcgaccg cgtcgacagc
     1621 gccttgtcgg gagcgagccg gtcgctcggc gcgaccggcg aaaagctgat cggggacctc
     1681 gacggaaaga tcgccgcgat cgacgcctcg ctgggggcgg cgaaccagcg cttcgaggct
     1741 tccggatcgg ccatcgcaga cggcgtcggc acgcagatcg agcgggtgga ggcggcgctc
     1801 gcctcggccg ggcagcggct gggcactacg agcgaccggc tggtcggcga gatcaaccgt
     1861 cagatcggcg cggtcgatgc ggcgctgatc tccgccaacg accgcttcgc ggcgaccggc
     1921 gaccgtctgg cggtcagcat cgacgagcgg atcgaaaacg tcgaaggccg gctcgcacgc
     1981 gccaacgacc ggctcaacac gaccggcgac cgtatcgccg gtaacgtcat ccagcagctc
     2041 ggcagcgtcg aggccgcact cgcgcttgcc gatgagcggc tcggcaccgc cggacgcgag
     2101 ctcgccgccc atgtcggcga gcagatgagc gccgtcgagc tgagcctgca gctggcgcag
     2161 gcccgcttcg ccgagtccgg cgaccatacg gccgagcgcg tccgcaccac gatggaaagc
     2221 ctgaaggacg tgatcgccac gacgggcgac gcgaccggaa cggccctcac ccagcgcggc
     2281 gaggaggtac ggagcctcat cgagaagaac atcgagcgcg tctccgaggt gctcgacaac
     2341 cgcggcggcg cgctcgccgc caccctcggc gagcgcagcg cggcgctcgg ctcgctgatc
     2401 gacacgcgcg tcgcccagat cggcgagacc ctggattccc gtctcaccgg gttcgaaagt
     2461 cgcctgaccg aaagcgccga ggccgccgcg atccgcctcg cctcttcggc cagcacgatc
     2521 gagattgcgg tcgaggggcg cctgtcgggg ttcgagaacc gcgtcaccgc ccgcatcggc
     2581 gaggtcaccc acaacttcgc ccagcgcgcc gacggcttcg ccaacacgct cgacgaccgg
     2641 ctggagcgca tcaactccac cctcgacacc cgcgcccgcc agatcagcga cacgcttgtg
     2701 gctcgcacgc gcgagatggc cgccgccttc cacgacggcc ataacgagat gtcgaccgct
     2761 ctcgacactc gcctgcaaac gctcggcgat catttgtcga cacggaccca ggagctcacc
     2821 gacacgatcg ccacccgaat gatggaaatc aaccagggct ttggcgagcg gctcctcggc
     2881 gtgcagacga gtttctccga tcgcgccaat tcgctgcaat cggcgcttgc cgcgaccggc
     2941 accatgctca acgaacgcac cgatcagctg aacgccacgc tggcagcg
//
LOCUS       DATABV010000035         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_976126, whole genome shotgun sequence.
ACCESSION   DATABV010000035 DATABV010000000
VERSION     DATABV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976126"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VHF88_01605"
     CDS             complement(<1..682)
                     /locus_tag="VHF88_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2160498.1"
                     /translation="MESVTVPAKSTEAPPTGIADLLPRAAAKHGQSPAVMFKDGASWR
                     TRSYAEVGDTVRELAKGLMALGVEKGDKVSILANTRPEWSYYDFAALSAGATVVPIYQ
                     TNSPEECQYVLHDSDAKVVVVEDEAQLAKVRAVRDTLPQLEHVILMIGSADDALSTAD
                     LVERGRALEDSAWTARYGSVTGDDICTFIYTSGTTGPPKGCVISHANYRAMVDMVVSE
                     DVVASGDVV"
     gene            complement(774..1631)
                     /locus_tag="VHF88_01610"
     CDS             complement(774..1631)
                     /locus_tag="VHF88_01610"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2160499.1"
                     /translation="MEARHEEGRFAGVGGLEIYWQAWLPGDPPRAIVLIAHGGAEHGG
                     RYAWTADQLTARGYAVYAIDHRGHGRSQGPRAYVDRVDNAVADLHALSDLARAGHPER
                     PVFLLGHSMGGLIALAYALRHQDELAGLVLSAPLAVIAANPATRLVSRVLSTVAPRLP
                     VYKIDGSTVSRDPEVVRAYDEDPLNHRGMLPARTIGELAATTARFPERLPELRLPILT
                     VYGTGDRLVDTAGSRLVDERSGSDDSTLIAYDGLYHEVLNEPERERVIADVVGWIDAR
                     APAPVTSFS"
BASE COUNT          238 a          591 c          610 g          257 t
ORIGIN      
        1 agacgacgtc gccgctcgcg acgacgtcct ccgacacgac catgtcgacc atcgcgcggt
       61 agttggcgtg cgagatcacg cagcccttgg gcgggcccgt agtccccgac gtgtaaataa
      121 acgtgcagat gtcgtcgccg gtcaccgacc cgtagcgggc ggtccaggca gagtcctcca
      181 gcgcgcggcc gcgctcgacg aggtcggcgg tcgacagcgc gtcgtcggcg ctgccaatca
      241 tcaggatcac gtgctcaagc tgcggcagcg tgtcgcgcac ggcacgcacc ttcgcgagct
      301 gcgcctcgtc ctcgacgacg accaccttgg cgtcggagtc gtgcaggacg tactggcact
      361 cctcgggcga gttggtctgg tagatcggca ccaccgtcgc gccggcggag agtgcggcga
      421 agtcgtagta gctccactcc ggccgggtgt tggcgaggat cgagaccttg tcgcccttct
      481 cgacgccgag cgccatcagg cccttggcca gctcgcgcac ggtgtcgccg acctcggcgt
      541 agctgcgcgt cctccagctc gcgccgtcct tgaacatcac cgcggggctt tggccgtgct
      601 tggcggccgc gcgcggcagc aggtcggcga tccccgtcgg gggtgcctcc gtcgacttcg
      661 cgggcacggt cacgctctcc actggtctct cctcgactgg ctacacgccg ggggatccct
      721 acgcccccgt gctggtcaca gagccagtct attgctgtga caaagctgtg tggtcaggag
      781 aagctcgtca ccggagccgg cgcccgggcg tcgatccagc caacgacgtc cgcgatcaca
      841 cgctcgcgct cgggctcgtt gaggacctcg tggtagagcc cgtcgtaggc gatcagcgtg
      901 ctgtcgtcgc tgcccgaccg ctcgtcgacg agccggctgc cggcggtgtc gaccagccgg
      961 tcgccggtcc cgtagaccgt caggatcggc agtctcagct cgggcagccg ctccgggaac
     1021 cgagcggtgg tggcggccag ctccccgatc gtgcgcgccg gcagcatgcc gcggtggttg
     1081 agcgggtcct cgtcgtaggc acgcacgacc tcgggatcgc ggctgacggt cgagccgtcg
     1141 atcttgtaga ccggcagccg cggcgcgacc gtcgacagga cgcggctcac gagccgcgtc
     1201 gccgggttgg ctgcgatcac ggcgagcggc gctgacagca ccagcccggc gagctcatct
     1261 tgatggcgca gtgcgtaggc gagcgcgatc agcccgccca tgctgtgacc gagcaggaag
     1321 acgggccgct cagggtggcc cgcccgggcg aggtcgctga gcgcgtggag gtcggcgacg
     1381 gcgttgtcga cgcgatcgac gtaggcccgc ggtccctggg agcgaccgtg gccgcgatgg
     1441 tcgatcgcgt agacggcata cccgcgggcg gtgagctggt cggcggtcca ggcgtagcgc
     1501 ccgccgtgct cggcgccgcc gtgggcgatc agcacgatcg cgcgcggcgg atcgcccggc
     1561 agccaggcct gccagtagat ctccaagccg ccgacgccgg cgaaccggcc ctcctcgtgg
     1621 cgcgcctcca tgcgcggatc aaatcacgct gccacgatgg ggggcgtgag cgaccaaggc
     1681 gacagccggg tgccgg
//
LOCUS       DATABV010000036         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_815571, whole genome shotgun sequence.
ACCESSION   DATABV010000036 DATABV010000000
VERSION     DATABV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815571"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..159
                     /locus_tag="VHF88_01615"
     CDS             1..159
                     /locus_tag="VHF88_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2160500.1"
                     /translation="MRATEEAVQIHGGYGFIEEYPVCRFYRDAKILTIGEGTDEVQQM
                     VIARALGA"
     gene            complement(246..1199)
                     /gene="meaB"
                     /locus_tag="VHF88_01620"
     CDS             complement(246..1199)
                     /gene="meaB"
                     /locus_tag="VHF88_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX2160501.1"
                     /translation="MSGARTAAELTDKLLAGDKRALARAISLVENDDPEGWALVREVY
                     PRTGKAAVVGFTGPPGVGKSTLIGALTSKFRERDRQVAVLSIDPSSPFTKGALLGDRI
                     RLTEHFLDPGVFIRSMANRGALGGLSEACLQAALLMDASGKDVVLLETVGVGQAEVDI
                     IDHADTVVLALMPGSGDSIQALKAGVMEIPDVIVVNKSDHPLTDTMIREVRGVLSLGP
                     QHGWRVPIVKTEASRGEGIDKLAEAIEAHREHIESEGTLAERRRRNLMNEVIALAAGR
                     LRRRLEESLQDDETVRELLDEVVARRIDPSSAATRLLEREL"
     gene            complement(1246..>2523)
                     /locus_tag="VHF88_01625"
     CDS             complement(1246..>2523)
                     /locus_tag="VHF88_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX2160502.1"
                     /translation="AGRAASSGSRASTPRRSAAILFATSLYSPHLVRPLASFAGWPME
                     RLRGLTGRLARENAMRKPGRTAVTSAALMIGLALVVFVTVFAAGINRSIASTIDTNFQ
                     GDITVQSTDGFSPIPNRIAREVSRVEGVGEVGTIRFSYGRPGGQSGQQRISGVDPATI
                     GGVFDFEFSEGSPETIARLGQRQAVVDEAYAKSNDLEVGDSVRMLTPANERPTFEIVG
                     AIKDRTDFLGSFVISQAALARQFGETRDTYVLARIAPDADPAAVEQRVKRLVDTRYPS
                     AEALDQEELKRSQEQQVSGLVNLIYALLALAIVVSIFGIINTLALSIHERTRELGMLR
                     AVGMTRRQVRRVIRYESVITALIGALLGTALGILFAALISRPLADEGFELAYPVVQLV
                     VILLLAALAGVLAAIAPARRAARLDVLDALAQE"
BASE COUNT          388 a          902 c          867 g          366 t
ORIGIN      
        1 gtgcgcgcca ccgaggaggc cgtccagatc cacggcggct acggcttcat cgaggagtac
       61 cccgtctgtc gcttctaccg cgacgcgaag atcctcacca tcggcgaggg caccgacgag
      121 gttcagcaga tggtcatcgc ccgcgccttg ggcgcctgac ctgcgaaggg cgcagccaga
      181 tcggtcctac ggcatcgtcg cggggctagc gggagcgagg tctggcgagt accgcagcca
      241 tggagtcaca gctcccgctc caggagccgg gtcgcggcgc tcgagggatc gatgcgccgc
      301 gcgacgacct cgtcgagcag ctcgcgaacc gtctcgtcgt cctgcagcga ctcctccaga
      361 cggcggcgca ggcgaccggc ggcaagtgcg atcacctcgt tcatcaggtt tcgccgacgg
      421 cgctccgcca gcgttccctc gctctcgatg tgctcgcgat gggcctcgat cgcctcggcg
      481 agcttgtcga tcccctcgcc gcgcgatgcc tcggtcttga cgatcggcac ccgccagccg
      541 tgctgcgggc cgagcgagag cacgccgcgg acctcacgga tcatcgtgtc ggtcagcggg
      601 tggtcggact tgttgacgac gatcacgtcg gggatctcca tcacgccggc cttgagcgcc
      661 tggatcgaat cgcccgagcc gggcatcagg gccagcacga ccgtgtcggc gtggtcgatg
      721 atgtccacct ccgcctggcc tacgccgacg gtctcgagca ggacgacgtc cttgccggag
      781 gcgtccatca gcagcgcagc ctgcaggcag gcctcggaga ggccgccgag cgcgccgcgg
      841 ttcgccatcg agcggatgaa gacgccgggg tcgaggaaat gctcggtcag ccggatccga
      901 tcgccgagca gcgcgccctt ggtgaacgga gacgaagggt cgatcgagag gacggcgacc
      961 tggcgatcgc gctcgcgaaa cttcgacgtc agcgcgccga tcagcgtcga cttgccgacc
     1021 cccggcggcc ccgtgaagcc cacgaccgcc gccttgcccg tgcgtggata gacctcgcgg
     1081 accagtgccc agccctcggg atcatcgttc tccacgagcg agatcgcacg cgcgagcgcc
     1141 cgcttgtcgc cggcgagcag cttgtcggtc agctcggcgg ctgtgcgggc gcctgacatg
     1201 ggagcggcag gctagtacag aggcgacccg cgacggagcg cgtgctcact cctgcgcgag
     1261 cgcgtcgagg acgtcgagcc tcgccgcccg gcgcgccggc gcgatcgccg ccagcactcc
     1321 cgcgagggcc gccagcagga ggatcacgac cagctgcacg accggatagg ccagctcgaa
     1381 tccctcgtcg gcgagcggcc gcgagatcag agcggcgaac aggatcccca gcgcggtgcc
     1441 caggagcgcc ccgatcagcg ccgtgatcac cgactcgtag cggatcacgc ggcgcacctg
     1501 cctgcgcgtc atgccgacgg cgcgcagcat ccccagctcg cgggtccgct cgtggatcga
     1561 cagcgcgagc gtattgatga tgccgaagat cgacaccacg atcgcgagcg ccagcagggc
     1621 gtagatcagg ttgacgagac cggacacctg ctgctcctgg gatcgcttga gctcctcctg
     1681 gtcgagcgcc tccgccgacg ggtagcgggt gtcgaccagg cgcttgaccc gctgctcgac
     1741 agccgctgga tccgcgtcgg gggcgatccg cgcgaggacg taggtgtcgc gcgtctcgcc
     1801 gaactgccgc gccagcgccg cctggctgat cacgaagctg ccgaggaagt ccgttcggtc
     1861 cttgatcgcc ccgacgatct cgaacgtcgg ccgctcgttg gccggcgtca gcatgcggac
     1921 gctatccccg acctcgagat cgttggactt ggcgtaggcc tcgtcgacca ccgcctgccg
     1981 ctggccgagc cgcgcgatcg tctccgggga cccctcgctg aactcgaagt cgaagacgcc
     2041 gccgatcgtc gcggggtcga cgcccgagat ccgctgctgg ccgctctgcc cgccgggacg
     2101 gccgtagctg aagcggatcg tcccgacctc gccgactccc tcgacccggc tcacctcgcg
     2161 cgcgatccgg ttcggtatcg gcgagaaccc gtcggtgctc tggacggtga tgtcgccctg
     2221 gaagttggtg tcaatcgtcg aggcgatcga ccggttgatc ccagcggcga agaccgtgac
     2281 gaagacgacc agcgcgagtc cgatcatcaa ggccgccgag gtcaccgcgg tgcgacccgg
     2341 cttgcgcatc gcgttctcgc gcgcgagccg gcccgtcagg ccgcgcagcc gctccatcgg
     2401 ccagcccgcg aacgaggcca gcggccgaac gaggtgcggg ctgtacagcg acgtcgcgaa
     2461 caggatcgcc gccgatcgtc gcggggtcga cgcccgagat ccgctgctgg ccgctctgcc
     2521 cgc
//
LOCUS       DATABV010000037         6255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_574902, whole genome shotgun sequence.
ACCESSION   DATABV010000037 DATABV010000000
VERSION     DATABV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574902"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VHF88_01630"
     CDS             complement(<1..588)
                     /locus_tag="VHF88_01630"
                     /inference="COORDINATES: protein motif:HMM:NF020060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160503.1"
                     /translation="MPSNPTAATPSTAGVEVERRYTQPGVHPFDAVEWEIRDAVIGDP
                     AKPAFEQRGVEFPKSWSQNATNIVAQKYFRGRLGSDERESSVRQMVGRVAGTIAGWGR
                     AGNYFASEADGDTFEAELTHILLHQLAAFNSPVWFNVGFEEQPQCSACFILSVQDTME
                     SILDWNTKEGKIFRGGSGSGINLSNIRGSMEHLAKG"
     gene            complement(786..1709)
                     /locus_tag="VHF88_01635"
     CDS             complement(786..1709)
                     /locus_tag="VHF88_01635"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2160504.1"
                     /translation="MRALVTGGSGFIGSNIVDGLLARGDEVTVLDDLSTGRRENLDGA
                     LANGAELVEGDIRDQAALVALAERVRPETIFHLAAQIDVRRSAADPALDARINVEGTI
                     NVLEAARTVGARVVNSSTGGAIYGEGRTIPAPEDHPVAPEAPYGQSKFAAEGYCELFR
                     RLHGVSTVSLRYGNVYGPRQDPLGEAGVIAIFCGKLLEGGQPTVFGDGLQTRDYVYVG
                     DVVAANLAAAGSDATGPINIGTGVESTVLELIEALAPQADGAFEPVMAPERPGEVRHI
                     ALDAARAGELLGWKAETGLADGLAVTLDSLR"
     gene            complement(1723..2376)
                     /locus_tag="VHF88_01640"
     CDS             complement(1723..2376)
                     /locus_tag="VHF88_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2160505.1"
                     /translation="MSTFVRPPSTQEAVLAELRRRIGAGELRPGEPIRAEQVGAELGV
                     SRVPVREALRTLEGEGQVVYEPHRGSFVADLSLDDLRELYRMRELLEQEAIRAALPRL
                     DGPALERLAGHVAEADAASERGDLATYAESNRRFHLELYEASGRPLLARTIRQLWDAS
                     DAYRALYANRESHRGTAAEHHRAILEALAAGDADAAVAAQDRHREHALAALTAILPD"
     gene            complement(2373..3503)
                     /locus_tag="VHF88_01645"
     CDS             complement(2373..3503)
                     /locus_tag="VHF88_01645"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2160506.1"
                     /translation="MASSTPLDGLLVADLSRVLAGPLVGMTLGDLGADVVKVESPSGD
                     DTRAWGPPFDAQGRATYHHAANRNKRSLALDLRDAGDNALARRLCERADVVIANFRPG
                     TLERFGLGFDQVSAANAGVVYCEISGFGEGAGRDLPGYDPLVQALGGLMSVTGPPDAP
                     SKAGVALIDVIAGLYATTAVLAALHERELSGAGQRVTIDLLHTTLAALANQATGWLGS
                     GAVPRRLGNVHPSIEPFGPCRAADGELMVCAGNDRQFGALAAAIGAPELAGDERFATN
                     ADRVANREALRPLIEQRLAARTIAQWEPLLAAAGVPAGPINDVAQAFELAERLGLDAV
                     DEHDGVRTVAFPARLSRTPATTRRRPPGLDEDGAALRRWLAG"
     gene            3560..4756
                     /locus_tag="VHF88_01650"
     CDS             3560..4756
                     /locus_tag="VHF88_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2160507.1"
                     /translation="MTATATAPPTPALDPHDPLAIDSLLDDEERMIRDTVRRYVRERF
                     LPTVAEHYEAGTFPDHVPADLGRLGLLGMHLEGYGCAGASATAYGLTCLELEAGDSGL
                     RSFCSVQGSLAMFAIHRWGSEEQKRSWLPRLAAGEAIGCFGLTEPDAGSNPGGMRTRA
                     RRDGDAWVLDGSKMWITNGSIADVAVVWARTDDGAVNGFLVERGTPGFSAPAMHGKLS
                     LRASVTSELVLDGVRLSDAARLPGAETLRGPLACLNEARYGVLWGAAGAARACYEAAL
                     SYSTEREQFGKPLAAFQLTQAKLAEMAVKVGQSTLLALHLGRLKDGGRLTPQHVSFGK
                     YHNVAAALEVARSARTVLGANGITLEYPVLRHANNLESVLTYEGTHEMHTLVLGQALT
                     GVAAFA"
     gene            4787..5413
                     /gene="tmk"
                     /locus_tag="VHF88_01655"
     CDS             4787..5413
                     /gene="tmk"
                     /locus_tag="VHF88_01655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX2160508.1"
                     /translation="MRGLFVTFEGIDRSGKTTQARLLLDALGDDAVGVREPGGTAAGE
                     RIRDLLKDPAVELSARSEALLFAAARAELVAGVIEPALAAGKVVVSDRFLDSSLAYQG
                     AARGLGIAEVERINGFATGGLRPDLTLLLEIDTAAAGRRGAVEDDRFEQEGAGLQRQV
                     AAAYEQLVAADPARWRRIDADRDPAAVHADVRAAVETARRAPAGDGAA"
     gene            5410..>6255
                     /locus_tag="VHF88_01660"
     CDS             5410..>6255
                     /locus_tag="VHF88_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2160509.1"
                     /translation="MIGEPAGVADGRAGTVLAGTESHPQARMTLSAALAGDASHAYLF
                     HGPAGVGKRTAARAFAAELLAAGSSDPDNARHRVLDGAHPDLTWVRPTGAHVMRTDDV
                     ADPVVSAATRTPFESQKRVFVLERVDTMNDEVANRLLKTLEEPAPFVHLILLTDSLGQ
                     VIPTVVSRCQLVRFDPLPAPRIAEALIAEGVDAERADPCARLALGNGARARFLASEEG
                     EALLGDVDRLVGAALAGGARHASEPEAWRGLLDRAEERRVAAEERVDTMNDEVANRLL
                     KTLEEP"
BASE COUNT          800 a         2289 c         2320 g          846 t
ORIGIN      
        1 gcccttcgcg aggtgctcca tcgagccgcg gatgttcgag aggttgatcc ccgagccgga
       61 gccgccgcgg aagatcttgc cctccttggt gttccagtcg aggatcgact ccatcgtgtc
      121 ctgcacgctg aggatgaagc aggccgagca ctgcggctgc tcctcgaagc cgacgttgaa
      181 ccagaccggc gagttgaacg ccgccagctg gtggagcagg atgtgcgtca gctcggcctc
      241 gaaggtgtcg ccgtcggcct cgctcgcgaa gtagttgccc gcgcggcccc agccggcgat
      301 cgtgccggcg acgcggccga ccatctggcg caccgaggac tcgcgctcgt cgctgccgag
      361 ccggccgcgg aagtacttct gggcgacgat gttcgtcgcg ttctgcgacc acgacttcgg
      421 gaactcgacg ccgcgctgct cgaaggccgg cttcgcgggg tcgccgatca cggcgtcgcg
      481 gatctcccac tcgacggcgt cgaacggatg cacgccgggc tgcgtatagc gccgctcgac
      541 ctcgactccg gcggtgctgg gggtggctgc ggtggggttg ctcggcatga ctctcctcgt
      601 cttctttgcg tccggattcc cggtccgcgg aggatgctca gaggcgctta ccgcaagccg
      661 ggaagcgatg gtgccgctcc cgccggacgg aagacgcacg aaaggcttcc aacagcaact
      721 ttagagcttc ggaatgcctt tgtctgtggg cagaggagcg ggttttcccc gctgtttgca
      781 gggtgtcagc gcagcgagtc gagcgtcacg gcgaggccgt cggcgaggcc ggtctcggcc
      841 ttccagccga gcagctcgcc ggcgcgcgcg gcgtcgagcg cgatgtggcg gacctcgccc
      901 gggcgctcgg gcgccatgac cggctcgaac gctccgtcgg cctgcggcgc cagcgcctcg
      961 atcagctcga gcaccgtcga ctcgaccccg gtgccgatgt tgatcgggcc ggtcgcgtcg
     1021 gagccggcgg cggccaggtt cgccgccacg acgtcgccga cgtagacgta gtcgcgcgtc
     1081 tgcaggccgt cgccgaagac cgtcggctgc ccgccctcga gcagcttgcc gcagaagatc
     1141 gcgatcacgc cggcctcgcc gagcgggtcc tggcgcggcc cgtagacgtt gccgtagcgg
     1201 agggagacag tcgagacgcc gtgcaggcgg cggaagagct cgcagtagcc ctcggcggcg
     1261 aacttcgact gaccgtaggg cgcctcgggc gccacggggt ggtcctccgg cgcgggaatc
     1321 gtcctgccct cgccgtagat cgcgccgccg gtcgacgagt tcacgacgcg cgcgccgacg
     1381 gtccgcgccg cctcgagcac gttgatcgtg ccctcgacat tgatgcgcgc gtcgagcgcc
     1441 gggtccgccg ccgagcgccg cacatcgatc tgggcggcga ggtggaagat cgtctccggc
     1501 cgcacgcgct cggcgagcgc caccagtgcg gcctggtcgc ggatgtcgcc ctcgaccagc
     1561 tcggcgccgt tggcgagcgc gccgtcgagg ttttcgcgcc gcccggtcga caggtcgtcg
     1621 agcacggtga cctcgtcgcc gcgggccagc aggccgtcga cgatgttcga gccgatgaat
     1681 ccggatcccc cggtgacgag tgcgcgcata gggcgcggca tcctagtcag gcaggatcgc
     1741 cgtcagcgcc gcgagcgcgt gctcgcggtg acggtcctgc gccgcgacgg ctgcgtcggc
     1801 gtcgcccgcg gcgagcgcct cgaggatcgc gcggtggtgc tcggcggcgg tgccacggtg
     1861 gctctcgcgg ttggcataga gcgcccgata ggcgtcgctg gcgtcccaga gctggcggat
     1921 cgtccgggcg agcagcggcc ggcccgacgc ctcgtagagc tcgagatgga agcggcggtt
     1981 ggactcggcg taggtcgcga ggtcgccgcg ctcgctcgcc gcgtcggcct ccgcgacgtg
     2041 gcccgccagc cgctcgagcg ccgggccgtc gagccgcggc agcgccgcgc ggatcgcctc
     2101 ctgctcgagc agctcgcgca tccggtacag ctcgcgcagg tcgtcgagcg acagatcggc
     2161 gacgaacgag ccgcggtgcg gctcgtagac gacctggccc tcgccctcga gggtccgcag
     2221 cgcctcgcgc accggcaccc gcgagacgcc gagctcggcg ccgacctgct cggcgcggat
     2281 cggctcgccg gggcgcagct cgccggcgcc gatccgccgc cgcagctcgg cgaggaccgc
     2341 ctcctgggtc gacggcggac ggacgaacgt gctcatccgg ccagccagcg gcgcagcgcg
     2401 gcgccgtcct cgtcgaggcc gggtggtcgc cggcgggtcg tcgcgggcgt ccgcgacagc
     2461 cgcgcgggga aggcgacggt gcgcacgccg tcgtgctcgt cgacggcgtc gaggccgagc
     2521 cgttcggcca gttcgaacgc ctgggccacg tcgttgatcg gcccggcggg cactccggca
     2581 gccgcgagca gcggctccca ctgcgcgatc gtccgcgccg cgagccgctg ctcgatcagc
     2641 ggtcgcagcg cctcgcggtt ggcgacgcgg tcggcgttgg tggcgaagcg ctcgtcgccg
     2701 gccagctcgg gcgccccgat cgccgccgcg agcgcgccga actggcgatc gttgccggcg
     2761 cagaccatca gctcgccgtc cgccgcgcgg caggggccga acggctcgat gctggggtgg
     2821 acgttgccga gccgccgcgg cacggcgccg ctcccgagcc agccggtcgc ctggttggcg
     2881 agcgcggcga gcgtcgtatg cagcaggtcg atcgtcacgc gctggccggc cccggatagc
     2941 tcgcgctcgt gcagggcggc gagcaccgcg gtcgtcgcgt agagcccggc gatcacgtcg
     3001 atcagcgcga cgccggcttt gctcggagcg tcgggcgggc cggtcacgct catcaggccg
     3061 ccgagcgcct ggacgagcgg gtcgtagccg ggcaggtcgc gtccggcgcc ctcgccgaag
     3121 cccgagatct cgcagtagac gacgcctgcg ttcgccgcgg agacctggtc gaagccgagg
     3181 ccgaaccgct cgagcgtgcc cgggcggaag ttggcgatga cgacgtcggc gcgctcgcag
     3241 agccggcgcg cgagcgcgtt gtcgccggcg tcgcgcagat cgagcgccag cgatcgcttg
     3301 ttgcggttcg cggcgtggtg gtaggtggcg cgcccctgtg cgtcgaacgg cggcccccag
     3361 gcgcgggtgt cgtcgccgct cggcgactcg accttgacga cgtcggcgcc gagatcgccg
     3421 agtgtcatcc caaccagcgg gccggccaag acgcgcgaca gatccgcgac cagaaggccg
     3481 tcgagggggg tcgaagaggc catcgagatg gatcgtatac cttctacgat tcaccccgct
     3541 ccctcccgga ggccccgtga tgaccgccac cgccacagcc ccgccgaccc cggccctcga
     3601 cccgcacgac ccgctcgcga tcgactcgct gctcgacgac gaggagcgga tgattcgtga
     3661 caccgtccgt cgctacgtcc gcgagcgctt cctgccgacg gtcgctgagc actacgaggc
     3721 ggggaccttc ccggaccacg tcccggccga cctcggccgc ctcggactgc tcgggatgca
     3781 cctcgagggc tacggctgcg ccggcgcctc ggcgaccgcc tacgggctga cctgcctcga
     3841 gctcgaggcg ggcgactcgg gcctgcgctc gttctgctcc gtccagggat cgctggcgat
     3901 gttcgcgatc caccgctggg gcagcgagga gcagaagcgg tcgtggctgc cacgcctggc
     3961 cgccggggag gcgatcggct gcttcggcct gaccgagccc gacgccgggt cgaaccccgg
     4021 cggcatgcgc actagagcgc gccgcgatgg ggacgcctgg gtgctcgacg ggtcgaagat
     4081 gtggatcacc aacgggtcga tcgccgatgt cgcggtcgtc tgggcgcgca ccgatgacgg
     4141 agcggtcaac ggcttcctgg tcgagcgcgg cacgcccggc ttctcggcgc cggcgatgca
     4201 cggcaagctg tcgctgcgcg cgagcgtcac cagcgagctc gtgctcgacg gcgtgcggct
     4261 ctccgacgcc gcccgcctgc cgggcgccga gacgctgcgc ggcccgctcg cgtgcctcaa
     4321 cgaggcgcgc tacggcgtgc tctggggagc cgccggcgcc gcgcgggcct gctacgaggc
     4381 cgcgctctcc tactcgaccg agcgcgagca gttcggcaag ccgctggcgg ccttccagct
     4441 gacccaggcc aagctcgccg agatggcggt caaggtcggc cagagcaccc tgctggcgct
     4501 gcacctcggt cggctcaagg acggcgggcg gctgacgccg cagcacgtct cgttcggcaa
     4561 gtaccacaac gtcgcggcgg cgctcgaagt cgcgcgcagc gcgcgcacgg tgctcggcgc
     4621 gaacgggatc acgctcgagt acccggtcct gcgccacgcc aacaacctcg agtcggtgct
     4681 cacctacgag ggcacgcacg agatgcacac gctggtcctc ggccaggcgc tgaccggcgt
     4741 cgccgcgttc gcctgagcgc ccgtccgcgc tggtagcgtc gcgccggtgc gcggcctgtt
     4801 cgtcaccttc gaggggatcg accgctcggg caagacgacc caggcgcgcc tgctgctcga
     4861 cgcgctcggc gacgacgcgg tcggcgtgcg cgagccgggc gggaccgcag ccggcgagcg
     4921 gatccgcgac ctgctcaagg atcccgcggt cgagctgtcg gcgcggagcg aggcgctgct
     4981 gttcgccgca gcgcgcgcgg agctggtcgc gggtgtgatc gagccggcgc tcgccgccgg
     5041 caaggtggtc gtctccgacc gcttcctcga ctcctcgctt gcctaccagg gcgcagcgcg
     5101 cgggctcggc atcgcggagg tcgagcggat caacggcttc gccaccggcg gactgcgacc
     5161 ggacctgacg ctgctgctgg agatcgatac ggccgccgcc ggccgccgcg gcgccgtcga
     5221 ggacgaccgc ttcgagcagg agggcgccgg gctccagcgc caggtcgccg ccgcctatga
     5281 gcagctggtc gccgccgatc cggcgcgctg gcggcggatc gacgccgacc gcgatcccgc
     5341 cgcggtccac gccgacgtgc gcgctgccgt cgagacggcc cgccgcgcgc cggcggggga
     5401 cggtgccgcg tgatcggcga gcccgccggg gtcgcggacg gccgagcagg aacggtgctc
     5461 gcgggcaccg aatcgcaccc ccaggcccgc atgacgctgt cggcggcgct cgccggcgac
     5521 gcgtcacacg cctacctctt ccacggcccc gcgggggtcg gcaagcgcac cgcggcgcgg
     5581 gcgttcgccg ccgagctgct cgccgccggc tcgtccgatc ccgacaacgc gcgtcaccgc
     5641 gtcctcgacg gcgcccatcc cgacctgacc tgggtccggc cgacgggagc ccacgtgatg
     5701 cgcacggacg acgtcgccga cccggtcgtc tcggccgcga cgcgcacgcc gttcgagtcc
     5761 cagaagcgcg tcttcgtgct cgagcgggtc gacacgatga acgacgaggt cgccaaccgg
     5821 ctgctcaaga cgctcgagga gccggcgccc ttcgtccacc tgatcctcct gaccgactcg
     5881 ctcggccagg tcatcccgac cgtcgtctcg cgctgccagc tggtccgctt cgacccgttg
     5941 ccggcgccga ggatcgcgga ggcgctgatc gccgaggggg tcgacgccga gcgggcggac
     6001 ccatgtgccc ggctcgcgct cggcaacggc gcgcgcgccc gcttcctcgc ctccgaggag
     6061 ggcgaggcgc tgctcggcga cgtcgaccgg ctggtcggcg ccgcgctcgc cggcggagcc
     6121 cgtcacgcga gcgagcccga ggcgtggcgc ggcctgctcg accgcgccga ggagcgccgg
     6181 gtggccgcgg aggagcgcgt cgacaccatg aacgacgagg tcgccaaccg gctgctcaag
     6241 acgctcgagg agccg
//
LOCUS       DATABV010000038         5070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2261702, whole genome shotgun sequence.
ACCESSION   DATABV010000038 DATABV010000000
VERSION     DATABV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2261702"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..798)
                     /locus_tag="VHF88_01665"
     CDS             complement(67..798)
                     /locus_tag="VHF88_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160510.1"
                     /translation="MRKLAVLPVALAAAAVAAAPAAAQVPVTEFKSSAKITPKKAGTK
                     KNPQGVKLSGSLEFRTITQGVEPPIITGGQVLIPKGGLYNGGKYRTCSKRTMARDDSV
                     QNCPKQSIMGKGSGVAFADTVDAKPDVVFVNGGAKTLWAFTTLYNPALVQEPIAIKIQ
                     KRSGKWAYRATFQVPKVLQVVAGVPVTLRSFKYNIGGKKYAKEFFATTSCPKNNKYPY
                     EAKAFYLYNDNTTSSSTFKSTVACR"
     gene            complement(950..1651)
                     /locus_tag="VHF88_01670"
     CDS             complement(950..1651)
                     /locus_tag="VHF88_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2160511.1"
                     /translation="MALDSSRAARAPHQLPPGRHGLARSFVARNQRERMLAAVAQVAS
                     TRGYGAMSVQDVVREAGVSRRTFYEQFKNKDDAFLAAYDEASGRLMTAIRAAIDAEEA
                     FEDKVSAGFRAFLELLAASPAFAKMCIVEVLAAGPEAIARRARTMEEFTGFFEAGAQE
                     TLVRENPPSPLIAETIVGGVYETVYRRIARGETDQLPRLLPDLVESALLPYVGEHKAT
                     AQRRRLLENRPPAAR"
     gene            1917..3347
                     /locus_tag="VHF88_01675"
     CDS             1917..3347
                     /locus_tag="VHF88_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2160512.1"
                     /translation="MTQRNDETPGATVGQPASAVSASRGPGRLIAAVAIGIALLALLA
                     IWVTRDDAHEYKLVFDNAGQMVKGDLVRIGGTPVGEVNAIELTDDGQAELTVSVAKEY
                     APLHSGTTATVRWQGLVGTANRYVDISPAPNFRAELEDGATIPGDKTKSIVEIDQLFN
                     TFTPKTREGLDHFIQGFADWYAGQERKANASAKYFSPALAQASKLFRELNRDSEDFEE
                     LLVETSKAMGAIEERSADLTGLVDNAGTTAAALSADTESLNQVLDDLPPALRQGSETF
                     AALRGPAIDDLERFVVESEPSAKVLPRFLRRFRTLAAESVPIFTDLRKMFNGPGEGND
                     LYDTMVDLPPLADMADGAFPRAQKSLRQSTPIWGFIRPYGPDLTAWLRSFGGAMAPYD
                     ANGHYARTIPVFDAFNFTDDANGGSLTPKPPAERGTSPYLSTGNLRRCPGSAAPPPAD
                     GSAPFVDMGELANPDCDPSQVIRSTP"
     gene            3344..4786
                     /locus_tag="VHF88_01680"
     CDS             3344..4786
                     /locus_tag="VHF88_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2160513.1"
                     /translation="MSGDPRARKLTSSRVIAIAVAAGIALGALFSIAAGGDEGDGYLV
                     RAVFDNGSFVIPGEDVKIAGVVVGAIHDVDLTEDNKAAVVLKVDDPAFVPFREDAQCQ
                     IRLQSLIGEQFIECVPTRPRKEGEPPKPELAMIEDGRGEGQHLLPADNNITPVGEDLL
                     RNINRLPQRQGLRLIINELGAGLAGNGDRLRDAVRRANPALKEFDGWVKVLAEQDRLL
                     GRLIDQSDTVLASWVQRREEMADFIDQSGATAVAAAERGDDIERNFARFPAFLRELKP
                     TADRFSGMADQMAPALNNLDRSSEAINAMVQGTGPFFDAATPALVSLGDFADRGRQLF
                     PAIRPLIGDLDELGRPLRPTMRDLAKLFGSFDDTGGIEELMQLIYYYTGTVNGVDAKG
                     HYVRTGLVIKCPARSPLQGGCAATLENYGDPVKAAADTSGLLQTLQRQFPPTKPGQVV
                     GDDAVVAGVDLNSEDAQPFLDYLLGERGGQ"
     gene            4783..>5070
                     /locus_tag="VHF88_01685"
     CDS             4783..>5070
                     /locus_tag="VHF88_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2160514.1"
                     /translation="MSRRPGTSVLSGSPVLVGAVTVIVTMVAVFLSYNANEGLPFVPT
                     YDLTANVPNANGLVRGNEVRMGGSRIGVVTAIETDPQDNGEVAANLTLELDE"
BASE COUNT          779 a         1810 c         1704 g          777 t
ORIGIN      
        1 gcccgccaaa ggcgggcgct tccggtcctg tatgtcgtgc gcgctgctag cgcagcgctg
       61 tcgccgctag cggcaggcga cggtgctctt gaacgtgctg ctcgacgtgg tgttgtcgtt
      121 gtacaggtag aaggccttgg cctcgtacgg gtacttgttg ttcttcgggc agctggtggt
      181 ggcgaagaac tccttcgcgt acttcttgcc gccgatgttg tacttgaagc tacgcagcgt
      241 taccggcacg ccggcgacga cctggagcac cttcggcacc tggaaggtcg cgcggtaggc
      301 ccacttgccg ctccgcttct ggatcttgat cgcgatcggc tcctgcacca gcgccgggtt
      361 gtagagcgtc gtgaatgccc acagcgtctt cgcgccgccg ttgacgaaga ccacgtcggg
      421 cttagcgtcg acggtgtcgg caaaggcgac gccgctgccc ttgcccatga tggactgctt
      481 ggggcagttc tggacgctgt cgtcgcgcgc catcgtgcgc ttcgagcagg tcctgtactt
      541 gccgccgttg tagaggccac ccttcggaat caggacctga ccgccggtga tgatcggggg
      601 ctcgacgccc tgggtgatcg tcctgaactc gaggctgccg ctgagcttga cgccctgcgg
      661 gttcttcttc gtaccagcct tcttcggcgt gatcttggcg gacgacttga actcggtgac
      721 gggcacctgg gccgcggcgg gcgccgccgc gacggcggcg gccgctaggg ccaccggtag
      781 gactgcgagc ttgcgcatct ctatttgctc ctcctgatcc gaaagtcttt atcgcggcag
      841 gttccttctc ccgccgagag cgcgatcgac cacgctaacg gacggcctcc cgtctgtgca
      901 aactgcaccg tacctacaaa cccgcgcgcg ggttgtaagt tgcggcgctt cagcgagcgg
      961 ccggtgggcg gttctcgagc agtcgccgcc gctgcgccgt cgccttgtgc tcgccgacgt
     1021 agggcagcag ggcggactcg acgaggtcgg gcagcaggcg cggcagctga tcggtctcgc
     1081 cgcgggcgat ccgccggtac accgtctcgt agacgccgcc gacgatcgtc tcggcgatca
     1141 gcggcgaggg cgggttctcc ctgaccagcg tctcctgggc ccccgcctcg aagaaccccg
     1201 tgaactcctc catcgtgcgg gcgcggcggg cgatcgcctc agggcccgcc gccagaacct
     1261 cgacgatgca catcttcgcg aacgccggcg aggccgcgag cagctcgagg aaggcgcgga
     1321 agcctgcgct gaccttgtcc tcgaacgcct cctcggcatc gatcgccgcg cggatcgcgg
     1381 tcatcaggcg gccgctcgcc tcgtcgtacg ccgcgaggaa tgcgtcgtcc ttgttcttga
     1441 actgctcgta gaacgtccgg cgtgagacgc cggcctcgcg cacgacatct tgcacgctca
     1501 tcgcgccgta accgcgcgtg ctcgccacct gggcgaccgc ggccagcatc cgctcgcgct
     1561 ggttgcgcgc cacgaagctc cgggccaggc cgtggcggcc gggcgggagc tggtgggggg
     1621 cacgcgctgc tctggagctg tctagggcca cgactggtct ggccaatata cacgcgcagg
     1681 tacatggtcg ccagtgctcg gatacgtcgc cttatcgcct cgattgggtc tcgatggcag
     1741 gtgctcgtgg cccgatttgg acaacccgaa ccagcgcacg cgtgcgggcg attcgtcaac
     1801 ctgccgtagg acatgggttc ccactggcag cgccatccag cgccatatac gatgcctggc
     1861 cggacggaga cgcaggctgg gttaggtcgc ggcgttgctc tccgtgggag gagaacttga
     1921 cccagcgcaa cgacgagacc cccggagcca cggttggcca gccagccagt gccgtgtccg
     1981 cgagccgggg ccccggccgt ctcatcgccg ctgtcgcgat cggcatcgcg ctgctcgccc
     2041 tgctcgcgat ctgggtcacg cgcgacgacg cgcacgagta caagctggtc ttcgacaacg
     2101 ccggccagat ggtcaagggc gacctcgtgc ggatcggtgg cacgccggtc ggcgaggtca
     2161 acgcgatcga gctgaccgac gacggccagg ccgagctgac cgtctcggtc gccaaggagt
     2221 acgcgcccct gcacagcggc actaccgcga cggtccgttg gcagggtctc gtcggcaccg
     2281 ccaaccgcta cgtcgacatc agcccggcgc ccaacttccg cgccgagctc gaggacggcg
     2341 ccaccatccc cggcgacaag acgaagtcga tcgtcgagat cgaccagctc ttcaacacct
     2401 tcacgccgaa gacgcgcgag ggcctcgacc acttcatcca gggcttcgcc gactggtatg
     2461 ccggtcagga gcgcaaggcc aacgcctcgg ccaagtactt ctcgccggcg ctcgcacagg
     2521 cgtcgaagct gttccgcgag ctcaaccgcg acagcgagga cttcgaggag ctgctcgtcg
     2581 agacctcgaa ggcgatgggt gcgatcgagg agcgcagcgc cgacctgacc ggcctggtcg
     2641 acaacgccgg cacgaccgcc gccgcgctga gcgccgacac cgagtcgctc aaccaggtcc
     2701 tggacgacct gccgcccgcg ctgcgacagg gcagcgagac cttcgcggcg ctgcgcggcc
     2761 cggcgatcga cgacctcgag cgcttcgtcg tcgagtcgga gccgagcgcc aaggtgctgc
     2821 cgcgcttcct gcgccgcttc cgcacgctcg cggctgagtc ggtgccgatc ttcaccgacc
     2881 tgcgcaagat gttcaacggc ccgggcgagg gcaacgacct ctacgacacg atggtcgacc
     2941 tgccgccgct cgcggacatg gccgacggcg ctttcccgcg cgcgcagaag tcgctgcggc
     3001 agtcgacgcc gatctggggc ttcatccgtc cctacggccc cgacctgacc gcctggctgc
     3061 gcagcttcgg cggcgcgatg gcgccatacg acgccaacgg ccactacgcc cgcacgatcc
     3121 ccgtcttcga cgccttcaac ttcaccgacg acgcgaacgg cggcagcctg acgccgaagc
     3181 cgcccgccga gcgcggcacg agcccctacc tgagcaccgg caacctgcgc cgctgccccg
     3241 gttcggccgc cccgccgccg gccgacggct cggcgccgtt cgtcgacatg ggtgagctcg
     3301 ccaaccccga ctgcgacccg tcgcaggtga tcaggagcac gccgtgagcg gcgatccacg
     3361 cgcgcgcaag ctgaccagca gccgggtgat cgcgatcgcc gtggcggcgg gcatcgcgct
     3421 cggcgcgctg ttcagcatcg ccgccggcgg cgacgagggc gacggctacc tcgtccgggc
     3481 cgtcttcgac aacggcagct tcgtgatccc cggcgaggac gtgaagatcg ccggcgtggt
     3541 cgtcggcgcg atccacgacg tcgacctgac cgaggacaac aaggccgccg tcgtcctgaa
     3601 ggtcgacgac ccggcgttcg tcccgttccg cgaggacgcc cagtgccaga tccggctgca
     3661 gtcactgatc ggcgagcagt tcatcgagtg cgtgccgacg cgcccacgca aggagggcga
     3721 gccgccgaag cccgagctgg cgatgatcga ggacggccgc ggcgaggggc agcacctgct
     3781 gccggccgac aacaacatca cgccggtcgg cgaggacctg ctgcgcaaca tcaaccgcct
     3841 gccgcagcgc cagggtttgc gtctgatcat caacgagctc ggcgcggggc tcgccggcaa
     3901 cggcgaccgc ctgcgcgacg cggtgcggcg cgccaacccc gcgctcaagg agttcgacgg
     3961 ctgggtcaag gtgctcgccg agcaggaccg cctgctgggc cggctgatcg accagtccga
     4021 cacggtgctc gcctcgtggg tccagcggcg cgaggagatg gccgacttca tcgaccagtc
     4081 cggcgccacc gcggtggcag ccgccgagcg cggcgacgac atcgagcgca acttcgcgcg
     4141 cttccccgcc ttcctgcgcg agctgaagcc gaccgccgac cgtttctccg gcatggccga
     4201 ccagatggcg ccggcgctga acaacctcga ccggtcgtcc gaggcgatca acgcgatggt
     4261 ccagggcacc gggccgttct tcgacgccgc cacgcccgcg ctcgtctcgc tcggtgactt
     4321 cgccgaccgt ggtcgtcagc tgttccccgc gatccggccg ctgatcggcg acctcgacga
     4381 gctcggacgg ccgctgcggc cgacgatgcg cgacctcgcg aagctgttcg gcagcttcga
     4441 cgacacaggc ggcatcgagg agctgatgca gctcatctac tactacaccg gcaccgtcaa
     4501 cggggtcgac gccaagggcc actacgtgcg caccggcctg gtcatcaagt gcccggcgcg
     4561 tagcccgctg cagggcggct gcgccgcaac gctcgagaac tacggtgatc cggtcaaggc
     4621 agccgccgac accagcggcc tgctccagac gctgcagcgc caattcccgc cgaccaagcc
     4681 cggccaggtc gtcggcgacg acgccgtcgt cgccggagtc gacctgaaca gcgaggacgc
     4741 ccagccgttc ctcgactacc tgctcggcga gcggggtggc cagtgagccg gcgcccaggg
     4801 acctcggtgc tctccggcag cccggtgctg gtcggcgcgg tgacggtgat cgtgacgatg
     4861 gtcgccgtct tcctctccta caacgccaac gagggcctgc cgttcgtgcc gacctacgac
     4921 ctgacggcca acgtgccgaa cgccaacggc ctcgtgcgcg gcaacgaggt ccggatgggc
     4981 ggctcgcgga tcggcgtcgt gaccgcgatc gagaccgacc cgcaggacaa cggcgaggta
     5041 gcggccaatc tgacgctgga gctcgacgag
//
LOCUS       DATABV010000039         5083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_575208, whole genome shotgun sequence.
ACCESSION   DATABV010000039 DATABV010000000
VERSION     DATABV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575208"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="VHF88_01690"
     CDS             <1..1907
                     /locus_tag="VHF88_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX2160515.1"
                     /translation="HRRNLVDQFIGEISDRGYKERLRPPLMGDRDDAEGPVTVETYQW
                     FVRNHKKISDAYSIVICDEAHTALGEKTSACIRAWTGPVFIGMTATGALIARHVADLF
                     PTQTSRFDLAQAARRGVIAPLRCLRIPPGPGVRTIAKVPLRKGEVDQDFDQEELAALL
                     DQEPFNVAIADLYKTRFSKVPGVLYTAGVQHAKNVAKALRAAGINARAVSGETPKREL
                     AEILAAFERGKVDVLCNAMLLAEGWNSPRATICMHLAPTASRRIYQQRVGRVTRRAPG
                     KEAGLVIDFVHPATTHDETIVTLHSLLDREVYRGGAIVVGPVRRGRGRKVRVERRIVP
                     VSDDPERRTQVLERELWRIAVENLDYPEQHAWAALAGARVNPQNWRRARSMLQNDRTK
                     ELRRRFLLTCVQRNRNSQLRLKALGEIADLRDGEAFDDAIDIVGSWSREDKRAGTKCL
                     LQALVDKRIGRRDQAQAWVWRLATYTRELHEEYAVQRWGETKRLLGLLVNSSGRAHGR
                     NARRLVHAARKQDRRLGVALLAAAVSHTPEGEEVLRGARMRMARKPSATARELLRNFP
                     KGGKRRRKRRKGKDGEGENGAQPAKRSRRRARKTDEATADKAATNGSEPQVDLAGAED
                     GELAVEAADE"
     gene            complement(1839..2564)
                     /locus_tag="VHF88_01695"
     CDS             complement(1839..2564)
                     /locus_tag="VHF88_01695"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX2160516.1"
                     /translation="MSFGHPHRHYRLTNSTNDRARELVEAGAPHGTVVTAEEQTAGRG
                     RQGRVWTAPAGKGLLYSAILRPLDERHLLLPLSVPLAVCEAAESLRPDIECQVKWPND
                     VWLEERKLAGILIEAKPQDGWAVIGVGLNLTVAADEFPADLKWPATSLHFDARTDACE
                     ALNRALDRWIAAPREEVLRAWRARDALRGREVSWQKGTGGAGAAEELRGVAEGIDDSG
                     DLLVRRLDGELAVLGAGEVHLRL"
     gene            complement(2600..4108)
                     /gene="cobA"
                     /locus_tag="VHF88_01700"
     CDS             complement(2600..4108)
                     /gene="cobA"
                     /locus_tag="VHF88_01700"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX2160517.1"
                     /translation="MSARPGVVYLVGAGPGDPGLMTARSLELIAAADAIFHDRLIPPG
                     ALDGARGDAELVYVGKAPGRPSLPQAEINERLIDAAREGKSVVRLKGGDPFVFGRGGE
                     EGEALREAGVEFEVVPGVTAGVAASAYAGIPVTHRNDASAVAFVTGHEDPEKPATALD
                     WEALARFPGTLVFYMGVKRLAENAAALIAAGRDANEPAAAVERGTTAGQRTIVATLGR
                     LAEAVARERIGAPALIVVGPVVERREALAWLKRRPLHGKRVVVTRARAQASGLAATLR
                     ALGAEAIELPAIRVEPRIETEDVRRAVERIGDYALVCLTSPNGVRLLFEALAATGRDA
                     RALAGATVAAIGSGTARALAERGIAADVVPDRFVAEALVEALAAVDVAGKRVLVARAA
                     EAREVLPDALRERGAEVDVVAVYETVREHPDDESVEAAQSADYVTFTSSSTVRNLVEA
                     LGDRFPGAARIVSIGPVTSEAVRAAGLEVDVEAKRHDVDGLVEALLQDAVGD"
     gene            complement(4158..5027)
                     /gene="hemC"
                     /locus_tag="VHF88_01705"
     CDS             complement(4158..5027)
                     /gene="hemC"
                     /locus_tag="VHF88_01705"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX2160518.1"
                     /translation="MIRIATRNSALAKAQARQVAELLGGAELVEVSSDGEPGDKGRFV
                     RGVERALLAGEAEVGVHSAKDVPAEMPEGLAIAAVPHREDPADVWVGAGSSLEEVPEG
                     SRVGTASLRRRAQLLAARPDLRVEELHGNVDTRLRKLAEGDLDGIVLAAAGLRRLGRE
                     GEAAFAIPAEAMVPAAGQGALVLQAREADGDSRERAAAIGDETALRELTAERTVVALL
                     EATCASPIGVHASVEGERIAVEAFVGLPDGSEWVRDRIEDDAAEPAAAGAELAGRLAA
                     AGARDILAGAETP"
BASE COUNT          728 a         1933 c         1782 g          640 t
ORIGIN      
        1 cccaccgccg caacctcgtc gaccagttca tcggcgagat ctccgaccgc ggctacaagg
       61 agcggctgcg gccgccgctg atgggcgacc gcgacgacgc cgaagggccg gtcacggtcg
      121 agacctacca gtggttcgtc cgcaaccaca agaagatctc cgacgcctac tcgatcgtca
      181 tctgcgacga ggcgcacacc gcgctcggcg agaaaaccag cgcctgcatc cgcgcctgga
      241 ccgggcccgt cttcatcggc atgaccgcca cgggcgcgct gatcgcgcgt cacgtcgccg
      301 acctcttccc gacccagacc tcgcgcttcg acctcgccca ggcggcgcgg cgcggcgtga
      361 tcgcgccgct gcgctgcctg cgcatcccgc cggggcccgg agtgcggacg atcgccaagg
      421 tgccgttgcg caaaggcgag gtcgaccagg acttcgacca ggaggagctc gccgcgctgc
      481 tcgaccagga gcccttcaac gtcgcgatcg ccgacctcta caagacgcgc ttcagcaagg
      541 tgccgggagt cctctacacg gcgggggtcc agcacgccaa gaacgtcgcc aaggcgctgc
      601 gcgccgccgg catcaacgcc cgcgccgtct ccggcgagac gccgaagcgc gagctggccg
      661 agatcctcgc cgccttcgag cgcggcaaag tcgacgtcct ctgcaacgcg atgctgctcg
      721 ccgagggatg gaacagcccg cgggcgacga tctgcatgca cctcgcgccg accgcctcgc
      781 gccgcatcta ccagcagcgg gtcggtcgcg tcacccggcg cgctcccggc aaggaggccg
      841 gcctggtgat cgacttcgtc cacccggcga cgacccacga cgagacgatc gtcaccctgc
      901 acagcctgct cgaccgcgag gtctaccgtg gcggcgcgat cgtcgtcggc ccggtgcgcc
      961 gcggacgcgg tcgcaaggtg cgggtcgagc ggcggatcgt ccccgtctcc gacgatcccg
     1021 aacgccgcac ccaggtgctc gaacgcgagc tgtggcggat cgcggtcgag aacctcgact
     1081 atcccgagca gcacgcctgg gcggccctgg ccggggcgcg cgtcaacccg cagaactggc
     1141 gacgggcccg ctcgatgctg cagaacgacc gcaccaagga gctgcgccgc cgcttcctgc
     1201 tcacctgcgt gcagcgcaac cgcaactcgc agctgcggct caaagcgctg ggggagatcg
     1261 ccgacctgcg cgacggcgag gccttcgacg acgcgatcga catcgtcggc agctggagcc
     1321 gcgaggacaa gcgggccggc accaagtgcc tgctgcaggc gctggtcgac aagcggatcg
     1381 gccggcgcga ccaggcgcag gcgtgggtct ggcggctcgc cacctacacc cgcgagctgc
     1441 acgaagagta cgccgtgcag cgttgggggg agacgaagcg gctgctcggc ctgctggtca
     1501 actcctccgg ccgggcgcac ggccgcaacg cccgccggct cgtccacgcc gcacgcaaac
     1561 aggaccgccg gctcggggtg gcgctgctcg cggcggcggt ctcgcacacg cccgaaggcg
     1621 aggaggtcct gcgcggggcg aggatgcgaa tggcccgcaa accgtcggcg acggcgcgcg
     1681 aactgctgcg caacttcccg aaaggcggca agcgccgccg caaacggcgc aaaggcaaag
     1741 acggggaggg cgagaacggc gcacagcccg cgaagcgctc ccgcaggagg gctcggaaaa
     1801 cggacgaggc caccgccgac aaggcggcga cgaacggctc agagccgcag gtggacctcg
     1861 ccggcgccga ggacggcgag ctcgccgtcg aggcggcgga cgagtaggtc gcccgagtcg
     1921 tcgatcccct cggcgacgcc ccgcagttcc tctgcagccc ccgcgccgcc ggtgcctttc
     1981 tgccaggaga cctcacgccc ccgcagcgcg tcgcgggctc gccaggctct gaggacctcc
     2041 tcgcgggggg cggcgatcca gcggtcgagt gctcggttca gcgcctcgca agcatccgtc
     2101 cgcgcatcga agtgcagcga ggtcgccggc cacttcaggt cggctgggaa ctcgtcggcc
     2161 gctacggtga ggttgaggcc gacgccgatc accgcccatc cgtcctgggg cttcgcctcg
     2221 atcaggatcc cggccagctt ccgctcctcg agccaaacgt cgttcggcca cttgacctgg
     2281 cactcgatgt cgggccgcaa cgactcggca gcctcacaga cggcaagcgg caccgagagc
     2341 ggcagcagca ggtgccgctc gtccagtgga cgcaggatcg ccgagtagag gaggcccttg
     2401 ccggcgggtg cggtccagac gcggccctgg cggccgcgcc cggccgtctg ctcctcggca
     2461 gtgacgaccg tgccgtgggg ggcgcccgcc tcgactagct cgcgggcgcg gtcgttggtc
     2521 gagttggtga ggcggtagtg gcggtgaggg tgaccgaagc tcattgctcg tttttacggc
     2581 ctatggaccg gcaaatcgat caatcgccga ccgcgtcctg gaggagcgcc tcgaccaggc
     2641 cgtcgacgtc gtgccgcttc gcttcgacgt cgacctcgag gccggcggcg cggacggcct
     2701 cgctggtgac cgggccgatc gagacgatgc gggcggcgcc ggggaagcgg tccccgagcg
     2761 cctcgacgag gttgcgtacg gtggacgagg aggtgaaggt gacgtagtcg gcgctctgcg
     2821 ccgcttcgac cgattcgtcg tcggggtgct cgcggacggt ctcgtagacc gcgacgacgt
     2881 cgacctcggc gccgcgctcg cgcagcgcgt cggggagcac ctcgcgcgcc tcggccgcac
     2941 gtgcgaccag gacccgcttg ccggcgacgt ccaccgcggc gagcgcctcg accagggcct
     3001 cggcgacgaa gcgatccgga acgacatcgg ccgctatgcc tcgctcggcc agcgctcgag
     3061 ccgtcccgga gccgatcgcc gccacggtcg cgccggccag cgcgcgggcg tcgcgccccg
     3121 tcgcggcgag cgcctcgaag agcagccgca cgccgttcgg actggtcagg cagacgagcg
     3181 cgtaatcgcc gatccgctcg acggcgcggc gcacgtcttc ggtctcgatc cgcggctcga
     3241 ctcggatcgc cggcagctcg atcgcctcgg cgccaagcgc ccgcagcgtc gcggcgagcc
     3301 cgctggcctg cgcccgggcg cgggtgacga cgacccgctt gccgtgcagc ggccgacgct
     3361 tgagccaggc cagggcctcc cgccgctcca ccaccggccc gacgacgatc agcgccggcg
     3421 cgccgatccg ctcgcgcgcg accgcctcgg cgagcctgcc gagcgtcgcg acgatcgtgc
     3481 gctgtcccgc ggtcgtgccg cgctcgaccg ccgccgcggg ctcgttcgcg tcgcggccgg
     3541 cggcgatcag cgccgcggcg ttctcggcca ggcgcttgac ccccatgtag aagacgagcg
     3601 tgccggggaa ccgggcgagc gcctcccagt cgagcgccgt cgccggcttc tcgggatcct
     3661 cgtggccggt gacgaaggcg accgccgagg cgtcgttgcg gtgcgtcacg gggatgccgg
     3721 cgtaggcgct tgccgcgacg cctgcggtca cccccggcac gacctcgaac tcgacgccgg
     3781 cctcgcggag cgcttccccc tcctcgccgc cccggccgaa gacgaacggg tcgccaccct
     3841 tgagacggac gacgctcttg ccctcccgcg ccgcgtcgat cagccgctcg ttgatctcgg
     3901 cctgcggtag cgacggcctg ccgggcgcct tgccgacgta gacgagctct gcgtcgcccc
     3961 gggcgccgtc gagggcgccg ggcgggatga gccggtcgtg gaagatcgcg tcggcggcgg
     4021 cgatcagctc cagcgatcgg gccgtcatca ggcccgggtc gccgggaccg gcgccgacca
     4081 ggtagacgac tcccggccgc gcgctcacgc gggtgtgcct tttgcacgta tgggggacat
     4141 aaagcgcacc gggagcctca cggggtctcc gccccggcga ggatgtcgcg ggcgccggca
     4201 gcggcgagcc gcccggcgag ctccgcgccg gcggcggccg gctccgccgc gtcgtcctcg
     4261 attcggtccc gcacccactc gctgccgtcc ggcagcccga cgaaagcctc gacggcgatg
     4321 cgctccccct cgaccgaggc gtgcacgccg atcgggctgg cgcaggtcgc ctcgagcagg
     4381 gcgacgacgg tccgctccgc ggtcagctcg cgcagcgccg tctcgtcgcc gatcgcggca
     4441 gcccgctcgc gcgagtcccc gtcggcctcg cgcgcctgca gcaccagcgc gccctgcccg
     4501 gccgcgggga ccatcgcctc ggccggaatc gcgaaggccg cctcgccctc gcggccgagg
     4561 cggcgcagtc cggcagcggc caggacgatc ccgtcgaggt cgccctcggc cagcttgcgc
     4621 agccgcgtgt cgacgttccc gtgcagctcc tccacccgca ggtcgggccg ggcggcgagc
     4681 aactgggccc gcctccgcag actggccgtg ccaacccgcg atccctccgg aacctcctcg
     4741 agcgacgacc cggccccgac ccagacgtcg gcgggatcct cgcgatgcgg caccgcggcg
     4801 atcgccagcc cctcgggcat ctcagccggc acgtccttgg ccgagtgcac cccgacctcc
     4861 gcctcaccag ccagcaacgc ccgctccacc ccccgcacaa accgcccctt gtcgccgggc
     4921 tccccgtccg aactcacctc gaccagctcc gctccaccga gcagctcagc cacctgtctc
     4981 gcctgagcct tggcaagcgc actgttgcga gtagcgattc ggatcatcag agaagggggt
     5041 gtgagcgact aagccggtcc cggtaggggt gaaacgtctt gta
//
LOCUS       DATABV010000040         7664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2021546, whole genome shotgun sequence.
ACCESSION   DATABV010000040 DATABV010000000
VERSION     DATABV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021546"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VHF88_01710"
     CDS             <1..559
                     /locus_tag="VHF88_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160519.1"
                     /translation="DPDPDPDPDPDPDPDPDPDPPSDPRAGPPKNADKAAPEVPVGNS
                     DVEPSPTLSPARAAAFARTQVARRLGKGKRKRAVKLGCKRRADGYRCTFRLGRAYRGA
                     LDVRDLDGRAGAARLTYTGSCGRLGTNGPSGIRTKNIECAVARRLIGNWVVRGKRPPR
                     AYDCDERRRTTCSARRKAFSFVRPG"
     gene            complement(556..1086)
                     /locus_tag="VHF88_01715"
     CDS             complement(556..1086)
                     /locus_tag="VHF88_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX2160520.1"
                     /translation="MVFVALLRGVNVGGKNRVEMARLRESFERLGFEDVRTYINSGNV
                     IFRYDGTADPETIERALAEEFGFEIRVTLRDKPSIDAVAAALPDDWVNDDTMKCDVMF
                     LWDGVDSHAALEDLTVKPGIDEVKPVEGALIWRVDRPAVTRSGLMKVAGSDLYKSMTV
                     RNCNTLRKLAALMDAV"
     gene            complement(1116..1919)
                     /locus_tag="VHF88_01720"
     CDS             complement(1116..1919)
                     /locus_tag="VHF88_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2160521.1"
                     /translation="MNASQVTSADGTAIAYERYGYGPPLIVVGGATCDRAKTRPTAQA
                     LGRRLATLNYDRRGRGASGDTAPYAVERELEDLAALIAAAGGSAALYGHSSGAGLVLH
                     AVAAGLPVTRFVLHDPPYVPDDEAMKREARAYATELDSLLAADRHGDAVALFFRTTGM
                     PDEMVDVVRQEPGWASLERLAPTLAYDSAVMGDASHGGVVPADVVARADKPGLVLCGG
                     ENPAFMREVGEQVSSLLPNGELRVLDGQGHVVPPELLAPIVAEYVVGEQ"
     gene            1981..2616
                     /locus_tag="VHF88_01725"
     CDS             1981..2616
                     /locus_tag="VHF88_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2160522.1"
                     /translation="MAGDDQGEGRSAQKRRAILAAARDIFLSKGYAGTSMDEVAEIAA
                     VSKQTVYKQFGAKQSLFETIVTEDIEATARTTQDMVTALGSSEQLERDLRRFARQHVA
                     DLTQPDLLRLRRLIVAEADRFPDLARTWFAHGPERGHALLAEQLETLARRGLLTIDDA
                     LLAAEHLNWLIVSIPLNKAMLLGGQTTFRAKELNRYADEGIRIFLAGYGAR"
     gene            complement(2784..6215)
                     /locus_tag="VHF88_01730"
     CDS             complement(2784..6215)
                     /locus_tag="VHF88_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160523.1"
                     /translation="MTLGRALLACFLAIAVLPASAGARDPFPDKVYAPFGPDHPAGTF
                     QPIDVDMHAGEKALVQTAGFEREPEAYPGTHARVWIDWGDGTESPGYIKYGPCLEPPG
                     PDVDERQPFLCERTEWQAIGSHTYSQPGEYQVTTRVIFVAATPKKYDVIRSTARVTTR
                     SAPGTAVPQAVTAPVEGLAATEATLRGEFHARGLHTGARFEYGETPQLGHVTGPAGAS
                     TADGFAQASFRLTGLRPGKTYYYRLVAERQAPNAARGDGETMSFVTPAAGGEPSLAVS
                     GQQGGNVELSATGYENERLIFVFDVDGNGSFETSCDDGKVIVAYPPGTQWTPAYVTKG
                     PDGKTTPARSGPTIGSHRASAAARKPAPKAPAPTTGPRPAPVLAMCPRPNIDFEAQVR
                     ALKDCPTTMRVGVLEAIFPPTAPKSACFKHVVAGKSERYEAPPNQAVQANGLHLEPEL
                     AANLLLDVNSYRFRSAAGSKSFMENPPLALVKVTIPGSSSNVTVNGKAVNLPVDVGAS
                     IDWDITKQGTVTSGGATKTFSLVSQKLLAKFAGLNAPQVGPIGLTARGALIPITLKLP
                     EKLVSGVSGAAILRTDNTEGLRFDGLSIHADRVPFGKLFSIAPLDLSYSRTDDRWSGR
                     AQIRLPNGAGVDARFVLENGQFKEAGGGARFVPGVPLGCCAALDYLGGSWKRTGSDGM
                     VISGDLGLIAGPRVAGRAAMGVGGTFSFFDRAPWAMSLSGSLSVVDQTLGTANVTYTH
                     ETSVTARVNLQRTFWNVLKVTGDLFADVYLRTNTWQAGGVADVCVDFKIANPCAGGGL
                     LMSSRAATGCLYFRMPGATYGDGIVKYWDRDDPEYYAGCGYAQLKQAAGAAQVGGERV
                     VTVPQGLPAYRFEATGTAGPPVVTVTGPDGRTITTDGPGVLGSQFFVYKDERTRTTYV
                     NAARPPAGKWRVVPAAGSALTALRTAEGLHEPKVRATVRRLRHRWELRYSAEGLGKGE
                     RLRFAERATQTVGGGRPRGEPGFVLGETRRSRGVMRFAPGEADAGVREIEATILGADG
                     LPRRILVAGRYRAPRPAVPRRPIVRLRQRGKRLVLTWSRDRRVLRYRVLVNESTGRRR
                     FFERPAKSRRLVLQRVSAGTRVTATVTGTTRSLRTGRAGRATLKVRRAAPRARKS"
     gene            complement(6212..>7664)
                     /locus_tag="VHF88_01735"
     CDS             complement(6212..>7664)
                     /locus_tag="VHF88_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2160524.1"
                     /translation="GAPPDCCPGALGEDGSDLLEAGVGPGEVDYSPREAPVTVVLPSG
                     DTTSSGNGEAGENDRLTRVRSVVGGLVGDVLVGGEGADSVTGGPGADTLVGGGGADVF
                     VQDQLRFPTVLRPTGPDGADELHGGGGADTVDYGRRDARVTVVLPDEGATTTGNGGNL
                     EGDQLTGIENVVTGDGDDIVTGSAEPNRIAVGLGVNSVAAGGGNDHLDAGAEAATDLL
                     DGGDGADTVDYGTRANGVTVSLPEGAAESTGNGEPGEADRLRAVEHVIGGAGPDVLTG
                     SSAPNSVDAGPGDDELWLEAGDTDELACGPGFDLAVLDAIDPPSLDCERTVLPPLAPL
                     DLARPPSGDAGLGAGLSQPSVPSSRGIVTPLPAAAGDLRLARTQRHPRVRGRLQVNAD
                     AARVEVRLTARLPSRPARGRARRSVRRSVVGRSVQHVAGPVRLFFRATLTGRARAALS
                     GRRSTPVRVRIVVTPTSGRSVSFSRTVVLKGAR"
BASE COUNT         1115 a         2760 c         2653 g         1136 t
ORIGIN      
        1 agatcccgat cctgacccgg accccgatcc tgacccggac ccagatcccg atccggaccc
       61 gccgagcgat ccgcgggcgg ggccgcccaa gaacgctgat aaggccgcgc cggaggtccc
      121 cgtggggaac tcggacgttg agcctagccc cacactgagc ccggcacgcg ccgccgcatt
      181 tgcgaggacg caggtcgcga gacggcttgg taagggcaag cgcaagcgcg ccgtgaagtt
      241 gggctgcaag cgccgagcgg acggctaccg ctgcaccttc aggctgggcc gcgcataccg
      301 aggcgccctc gatgtccgcg acctcgacgg gcgggccggt gccgcccgcc tgacctacac
      361 aggcagctgc ggtcgtctcg gcaccaacgg gcccagcggg atcaggacga agaacatcga
      421 gtgcgcggtc gcccgacgct tgatcggcaa ctgggtcgta cgtggcaagc ggccgccacg
      481 agcctacgac tgcgacgagc gcaggcggac gacctgcagc gcccgacgca aggcgttcag
      541 tttcgtgcga ccgggctaga cggcgtccat caacgcggcc agcttgcgca acgtgttgca
      601 gttccgcacg gtcatgctct tgtagaggtc gctgccggcg accttcatca agccgctgcg
      661 ggttaccgcc ggtcggtcca ctcgccagat cagcgccccc tcgacgggct tgacctcgtc
      721 gatgccgggc ttgacggtca ggtcctccaa tgccgcgtgg ctgtcgacgc catcccagag
      781 gaacatgacg tcgcacttca tcgtgtcgtc gttgacccag tcgtcgggca gtgcggccgc
      841 gacggcgtcg atgctcggct tgtcgcgcag cgtcaccctg atctcgaagc cgaactcctc
      901 cgcgagggcg cgctcgatcg tctccgggtc ggccgtcccg tcgtagcgga agatcacgtt
      961 gcctgagttg atgtaggtcc ggacgtcctc gaacccgagc cgctcgaacg actccctcag
     1021 ccgagccatc tcgacccggt tcttgccacc gacgttgacg cccctcagca gtgcaacgaa
     1081 gaccatgccg ggaggttggc agacgagcgc cggacctact gctcgccgac gacgtactcg
     1141 gcgacgatcg gagcgagcaa ctcgggcggg acgacgtggc cctgaccgtc gagcacacgg
     1201 agctcgccgt tcggcaggag gctggagacc tgctcgccaa cctcgcgcat gaaggcgggg
     1261 ttctcgccgc cgcagagcac gaggccgggc ttgtcggcgc gcgcgaccac gtctgcgggc
     1321 acgacgccgc catggctggc gtcgcccatc accgccgagt cgtacgcgag cgtcggcgcg
     1381 agccgctcga ggctcgccca gcccggttcc tggcgcacca cgtcgaccat ctcgtccggc
     1441 attccggtgg ttcggaagaa cagcgcgacc gcgtcgccgt gacggtcggc cgccagcagg
     1501 ctgtcgagct cggtggcgta ggcccgggcc tcgcgcttca tcgcctcgtc gtcggggacg
     1561 tagggcgggt catggagcac gaaccgcgtg accggcagac cggccgcgac cgcgtgcagg
     1621 acgaggccgg cgcccgagga gtgaccgtag agggcggcgc tcccgccggc cgcagcgatc
     1681 agcgcggcga ggtcctcgag ctcgcgctcg acggcgtacg gtgcggtgtc gccgctggcg
     1741 ccgcggccgc gtcggtcgta gttgagcgtc gcgagccgtc gcccgagcgc ctgcgccgtc
     1801 gggcgcgtct tggcgcgatc gcacgtcgcc ccacccacca cgatcagcgg cggtccgtag
     1861 ccgtaacgct cgtaggcgat cgccgtcccg tccgcggatg tcacctggct cgcgttcatg
     1921 cttctagaac tagaccgtac agtacttacc tggaactgga cggtcaagta ccgtctcgac
     1981 atggcagggg acgatcaagg cgagggccgg tccgcgcaga agcggcgagc gatccttgct
     2041 gccgcacgcg acatcttctt gtccaagggc tacgccggca cgagcatgga cgaggtcgct
     2101 gagatcgcgg cggtctccaa gcagacggtc tacaagcagt tcggcgccaa gcagtcgctg
     2161 ttcgagacga tcgtcaccga ggacatcgag gcgaccgccc gcactaccca ggacatggtc
     2221 accgcgctcg gctcgagcga gcagctcgag cgcgacctgc gccgcttcgc gcgccagcac
     2281 gtcgccgatc tgacccagcc cgacctgctg cgactccgcc gcctgatcgt cgccgaagcc
     2341 gaccggtttc ccgacctggc gcgaacctgg ttcgcacacg gccccgagcg cggccacgcg
     2401 cttctcgccg agcagctcga aacgctcgca cgacgcggcc tgctgacgat cgacgacgcg
     2461 ctcctcgcgg ctgagcacct gaactggctg atcgtctcga tccccctcaa caaggcgatg
     2521 ctgcttggcg ggcagaccac gttccgcgcc aaagagctca accgctacgc cgacgagggc
     2581 atccgaatct tcctcgccgg gtacggcgct cgatagcccc cccaagcgca acacgggcgg
     2641 gcctgccgga cgactccctc agccgagcca tctcgacgcg gttcttggcg cctccatctc
     2701 gatcgtgaag gtcgagacac cgtgctgccc ggtcacgacc cggacatcct gcgtccggga
     2761 ttgacgacgg tctgaacggc tcgctagctc ttccgggcgc gcggcgccgc gcggcgcacc
     2821 ttcaaggtcg ctcggccggc gcgacccgtg cgcaggcttc gcgtggtccc ggtgacagtc
     2881 gcggtcaccc gagtgcccgc cgagacccgc tgcagcacta ggcgccggct cttcgccggt
     2941 cgctcgaaga accgacggcg ccctgtcgac tcgttcacga gcacgcggta gcggagcacc
     3001 cggcggtccc gtgaccaggt gagcacgagc cgcttgccgc gttggcgcag tcggacgatt
     3061 gggcgcctgg gaacggccgg gcgcggcgcc cgatatcggc ccgcgacgag aatgcgtcgc
     3121 ggcagtccgt ccgcgcccag aatcgtcgcc tcgatctcgc gcacgccggc gtcggcctcg
     3181 cccggcgcga agcgcatcac accgcggctg cggcgcgtct cgcctaggac gaacccaggc
     3241 tcgccgcggg gacggccgcc cccgaccgtc tgcgtcgccc gctcggcgaa gcgcagccgc
     3301 tcgcccttcc ccaggccctc cgcgctgtag cgcagctccc agcgatggcg gagacgccgc
     3361 acggtggccc gcaccttcgg ctcgtgcaac ccctcggcgg tacgcagagc tgtgagagcc
     3421 gagcccgcgg ccggcacgac gcgccacttg ccggcgggcg gccgcgcagc gttcacgtag
     3481 gtggtgcgcg tgcgctcgtc cttgtagacg aagaactggc ttcccagcac gccggggccg
     3541 tccgtcgtga tcgtgcgacc gtcggggccg gtgaccgtga ccaccggagg gcccgcggtc
     3601 cccgtcgcct cgaaccgata tgcgggcagg ccctgcggca cggtgacgac gcgctcaccc
     3661 cctacctggg cagccccagc tgcctgcttg agctgcgcgt aaccgcatcc cgcgtagtac
     3721 tccggatcgt cacggtccca gtacttcacg atcccgtcgc cgtacgtcgc tcccggcatc
     3781 cggaaataga ggcatccggt tgcggcgcgg ctggacatca gcaggccacc cccggcgcag
     3841 ggattggcga tcttgaagtc gacgcagacg tccgcgaccc caccggcctg ccaggtgttc
     3901 gtccggaggt agacgtcggc gaacaggtcg ccggtcacct tcaagacgtt ccagaacgtc
     3961 ctctgcaaat tcaccctggc cgtcacgctc gtctcgtggg tgtatgtgac gttggccgta
     4021 ccgagcgtct ggtcgacgac cgagaggctg ccgctcagcg acatggccca cggcgcccgg
     4081 tcgaagaagc tgaaggtccc gccgacgccc atcgcggcgc gtccggcgac cctgggcccc
     4141 gcgatcaggc cgaggtcacc gctgatcacc atcccgtcgc tgcccgtccg cttccagctg
     4201 ccgccgaggt agtcgagcgc ggcacaacat ccgagcggga ctccgggcac gaagcgggcc
     4261 ccgcccccgg cctccttgaa ctggccgttc tcaagcacga atcgcgcgtc gaccccggcg
     4321 ccgttgggaa ggcggatctg cgcgcggccg ctccagcgat cgtccgtgcg ggagtagctg
     4381 aggtcgagcg gagcgatcga gaagagcttc ccgaagggca cccggtcggc atggatgctg
     4441 aggccgtcga accgcaggcc ctcggtgttg tcggttcgga ggatcgccgc gccgctcacg
     4501 ccgctgacca gcttctcggg gagcttcagc gtgatcggga tgagagcgcc gcgggcggtg
     4561 agcccgatcg gcccgacctg gggcgcgttg aggccggcga acttcgcgag gagcttctgc
     4621 gagaccaggc tgaacgtctt ggtcgcgccg cccgaggtga ccgtcccctg ctttgtgatg
     4681 tcccagtcga tggaggctcc gacatccacc ggcaggttca ccgccttgcc attcacggtc
     4741 acgttggacg aggaccctgg gatcgtgacc ttcaccaggg caagcggcgg gttctccatg
     4801 aagctcttcg agcccgcggc cgatcggaag cgatagctgt tgacgtcgag cagcaggttc
     4861 gccgccagct cgggctcgag gtgtagacca ttggcctgca ctgcctggtt cggaggggcc
     4921 tcgtagcgct cggacttgcc ggcaacgacg tgcttgaagc aggcgctctt cggcgcggtc
     4981 ggcgggaaga tcgcctcgag cactcccacc cgcatggtcg tcgggcagtc cttcagcgcg
     5041 cgaacctgcg cctcgaagtc gatgtttggt cgcgggcaca tggcgagcac gggcgcgggc
     5101 cggggaccgg tggtgggcgc cggcgctttc ggcgcgggct tcctcgccgc ggcggaggcg
     5161 cggtgggagc cgatcgtggg accgctgcgc gccggggtcg tcttgccatc gggccccttc
     5221 gtgacgtaag ccggcgtcca ttgcgtcccg ggcgggtagg cgacgatcac cttgccgtcg
     5281 tcgcacgagg tctcgaagct gccgtttcca tccacgtcga agacgaagat caggcgctcg
     5341 ttctcgtacc cggtggccga cagctcgacg ttgcccccct gctgccctga caccgcgagc
     5401 gagggttcgc cgccggcggc gggggtgacg aaggacatcg tctctccgtc gccgcgcgcg
     5461 gcattcggtg cctgacgctc ggcgaccaac cggtagtagt aggtctttcc gggtcgcagg
     5521 ccggtgagcc tgaacgaggc ttgcgcgaat ccgtcggctg tgctcgcgcc cgcgggcccc
     5581 gtgacgtgcc cgagttgcgg cgtctcgccg tactcgaagc gcgcgcccgt gtgcaggccg
     5641 cgcgcgtgga actcgccgcg cagcgtcgcc tcggtcgccg ccaggccctc gaccggcgcc
     5701 gtgaccgcct gcggcacggc ggttccggga gcggagcgcg tagtcacccg ggcggtgctt
     5761 cggatgacgt cgtacttctt cggcgtcgcc gcgacgaaga tgacccgagt ggtcacctga
     5821 tactcgccgg gctgggaata ggtgtgagat ccgatcgcct gccattcggt ccgctcgcag
     5881 aggaacggct ggcgctcgtc gacatccggc ccgggcggct cgagacaagg tccgtacttg
     5941 atgtagccgg gcgactccgt gccgtccccc caatcgatcc agacgcgtgc gtgcgtcccg
     6001 ggataggcct cgggctctcg ctcgaacccg gccgtttgga cgagggcctt ctcacccgcg
     6061 tgcatgtcga cgtcgatcgg ttggaaggtc cccgccgggt ggtcgggtcc gaacggcgcg
     6121 tagaccttgt ccgggaaggg gtcgcgtgct ccggcgctcg ccgggaggac ggcgatcgcc
     6181 aggaagcagg cgagcagtgc gcgacccaag gtcatcgagc ccccttcagg acaacggtgc
     6241 ggctgaacga aacgcttctc ccgctcgtcg gcgtgacgac gatgcgcacc cggaccggcg
     6301 tcgagcggcg accgctcagg gccgcgcgcg cacgaccggt cagggtcgcc cggaagaaga
     6361 ggcgaaccgg gccggcgacg tgctgcacgg agcgacctac gacggagcgg cgaaccgaac
     6421 ggcgcgctcg tccacgcgct ggccgggacg gcaggcgagc ggtcaaccgg acctccacgc
     6481 gggcggcatc ggcgttgacc tgcaggcgcc cccgcactcg cggatggcgt tgggtgcggg
     6541 cgagtcgcag gtcgcccgcg gcagcgggta ggggcgtgac gatcccgcgt gagctaggaa
     6601 cggacggctg gctgagcccc gcgcccaggc cagcatcccc actcggcgga cgggcgaggt
     6661 cgagtggcgc gagcggcggt agcacggttc gctcgcagtc gaggctcggc gggtcgatcg
     6721 catcgagcac cgcgagatcg aatcccgggc cacaagcgag ctcgtcggtg tcgccagcct
     6781 cgagccacag ctcgtcgtcg ccggggccgg cgtcgaccga gttcggcgcc gagcttccgg
     6841 tcaggacgtc gggacccgcc ccaccgatca cgtgctcgac ggcgcgcagc ctgtcggcct
     6901 cccccggctc gccgttgccc gtagactctg cagcgccctc gggtagggag actgtcaccc
     6961 cattggcgcg cgtcccgtag tcgaccgtgt ccgcgccgtc gccgccgtcg agcaggtcgg
     7021 tcgccgcctc ggccccggcg tccagatgat cgttgccacc gcccgcggcg acgctgttga
     7081 ctccgaggcc gaccgcgatg cggttcggct cggcgctgcc tgtgacgatg tcgtcgccgt
     7141 cgccggtcac gacgttctcg atcccggtga gctggtcccc ctcgaggttc cctccgttgc
     7201 cagtggtcgt cgccccctcg tcggggagaa ccacggtcac gcgagcgtcg cggcggccat
     7261 agtcgactgt gtcggcgcca ccgccaccgt gcagctcatc ggcgccatcg ggaccggtcg
     7321 gccgcaggac ggtcgggaac cggagctggt cttgcacgaa gacgtccgcg ccaccgccgc
     7381 ccaccagcgt gtcggcccca ggaccgccgg tcacggagtc cgcgccctcg ccgccgacca
     7441 ggacgtcgcc caccaacccg cccacgacgg agcgtacgcg tgtgagccgg tcgttctcgc
     7501 cggcttcgcc gttgccgctc gacgtcgtgt caccgctggg caggacgacc gtcaccggcg
     7561 cctcgcgggg ggagtagtcg acctcgccag ggccgactcc ggcctccagc aagtccgagc
     7621 cgtcctcgcc gagcgcgccg ggacagcagt ccggcggcgc gccc
//
LOCUS       DATABV010000041         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1700321, whole genome shotgun sequence.
ACCESSION   DATABV010000041 DATABV010000000
VERSION     DATABV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700321"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..305
                     /gene="trxA"
                     /locus_tag="VHF88_01740"
     CDS             24..305
                     /gene="trxA"
                     /locus_tag="VHF88_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX2160525.1"
                     /translation="MVEADKPVLVDFWAPWCGPCRVVAPHLEELAGERDDLKIVKLNV
                     DENPQTAASFNVMSIPTLLLFKNGAVAHQIVGALPKSRLVQELEPALAA"
     gene            308..520
                     /locus_tag="VHF88_01745"
     CDS             308..520
                     /locus_tag="VHF88_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX2160526.1"
                     /translation="MPIVTIEWYEGRSAEQKRELAERLTDLLVEVGKTQPEHVWIRFK
                     DSKQSDWAMGGKVQGTGGEPALGPPD"
     gene            complement(522..>1627)
                     /locus_tag="VHF88_01750"
     CDS             complement(522..>1627)
                     /locus_tag="VHF88_01750"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEX2160527.1"
                     /translation="TATAIDRLDDFDTTAAGRAVAAFVDELSNWYVRRSRRRFWDGDP
                     AAFATLRECLVTTAKLLAPLTPFVADAIYGNLDGAEPSVHLCDYPEPDAALRDEQLEQ
                     QMGVVRDAVELGRAARAAAKAKVRQPLSEAVVVAADREREAIGRFEDLVLDELNVKRV
                     RYVSQADELGRFELKPNYRTLGPRFGKDMPKVADAVGGLDADRAAATLRDGGEIHVAI
                     DGRDHPLGPDDVQLALQPLEGYEVERAGTHAVALNLELDDDLRREALAREIVHAVQNA
                     RKEAGLDVQDRISLSLAGDDALLEAVRAHEDYVKGETLVVGAVDYAAADQLAAGVRPR
                     RDPLGPGPSLLAGQPDGMISIEGRPLLIQVKQA"
BASE COUNT          229 a          546 c          615 g          237 t
ORIGIN      
        1 ccgacaacaa cttccaagcc gaggtggtgg aggccgacaa gccagtgctc gtcgacttct
       61 gggcaccgtg gtgcggtccg tgccgcgtcg tcgccccaca cctcgaggag ctcgcgggcg
      121 agcgcgacga cctgaagatc gtgaagctca acgtcgacga gaacccgcag accgcggcga
      181 gcttcaacgt gatgtcgatc ccgacgctgt tgctgttcaa gaacggcgcg gtggcgcacc
      241 agatcgtcgg ggcgctgccg aagagccggc tggtgcagga gctcgagccg gccctcgcgg
      301 cgtagcgatg ccgatcgtca cgatcgagtg gtacgagggt cgctcggccg agcagaagcg
      361 cgagctcgcc gagcggctca cggaccttct cgtcgaggtc ggcaagaccc agcccgagca
      421 cgtctggatc cgcttcaagg actccaagca gtccgactgg gcgatgggcg gcaaggtcca
      481 gggcaccggc ggcgagccag cgctcggccc gcccgactga gtcaggcctg cttcacctgg
      541 atcagcagcg gtcggccctc gatcgagatc atcccgtcgg gctgaccggc taggaggctc
      601 ggaccgggcc cgagcgggtc gcggcgaggt cgtacgcccg ctgcgagttg gtcggccgcc
      661 gcgtaatcga cagcgccgac tacgagcgtc tcacccttga cgtagtcctc atgcgcacgg
      721 accgcctcga gcagggcgtc gtcaccggcg agggagagtg agatccggtc ctggacgtcg
      781 aggccggcct ccttgcgcgc gttctggacg gcgtggacga tctcgcgggc gagcgcctcg
      841 cggcggaggt cgtcgtcgag ctcgaggttc agtgcgacgg cgtgcgttcc ggctcgctcg
      901 acctcgtagc cctcgagcgg ctgcagcgcg agctggacgt cgtcggggcc gagcgggtgg
      961 tcgcggccgt cgatcgctac gtggatctcg ccgccgtcgc gcagcgtggc cgcggcgcgg
     1021 tcggcgtcga ggcctccgac ggcgtcggcg accttcggca tgtccttgcc gaagcgcggc
     1081 ccgagcgtgc ggtagttcgg cttcagctcg aagcggccga gctcgtcggc ctgcgagacg
     1141 tagcggacgc gcttgacgtt gagctcgtcg agcacgaggt cctcgaagcg gccgatcgcc
     1201 tcgcgctcgc ggtcggcggc gacgacgacc gcctcgctga gcggctgacg gaccttcgcc
     1261 ttggcggcgg cgcgcgcggc gcgccccagc tcgacggcgt cgcggacgac gcccatctgc
     1321 tgctcgagct gctcgtcgcg cagcgcggcg tcgggctcgg ggtagtcgca gagatggacc
     1381 gacggctcgg cgccgtcgag gttgccgtag atcgcgtcgg cgacgaacgg ggtcagcggc
     1441 gcgagcagct tcgcggttgt gaccaggcac tcgcgcagcg tcgcgaaggc ggctgggtcg
     1501 ccgtcccaga agcggcgccg cgagcggcgc acgtaccagt tcgacagctc gtcgacgaag
     1561 gcggcgaccg cccggccggc ggcggtggtg tcgaagtcgt cgaggcggtc gatcgcggtc
     1621 gcggtcg
//
LOCUS       DATABV010000042         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2101624, whole genome shotgun sequence.
ACCESSION   DATABV010000042 DATABV010000000
VERSION     DATABV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101624"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VHF88_01755"
     CDS             <1..363
                     /locus_tag="VHF88_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160528.1"
                     /translation="TREPPSSMHDVDFADVVGRRPAVLLFATAALCQSRVCGPVIDVT
                     EQVKAERGDEVAFVHQEIYVDNQIDKGQRPSVAAFGLPTEPWLFAVDADGRIAARIEG
                     AFSADELHRAIDAAVGKS"
     gene            406..479
                     /locus_tag="VHF88_01760"
     tRNA            406..479
                     /locus_tag="VHF88_01760"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Ile,seq:cat)
     gene            709..921
                     /locus_tag="VHF88_01765"
     CDS             709..921
                     /locus_tag="VHF88_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160529.1"
                     /translation="MAISVRKLAIGEIVEVDGRRYEVVPTRDGELTLETPMTPMDELH
                     AIDGTKPASAEDFERLSGHVPYDDEG"
     gene            949..1323
                     /locus_tag="VHF88_01770"
     CDS             949..1323
                     /locus_tag="VHF88_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160530.1"
                     /translation="MSAEVVFGLRPWEEEVERYHQLSPPRIRAENARKMIEAGDLKLD
                     WLNCKPDGPVPLPGCRKLYVPLGKQAASEAPYGFVFLLVQRRDGTLAWTFVAFGERHP
                     SNPKTRTVYERAYKRINGRYPS"
     gene            complement(1447..1995)
                     /gene="rfbC"
                     /locus_tag="VHF88_01775"
     CDS             complement(1447..1995)
                     /gene="rfbC"
                     /locus_tag="VHF88_01775"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX2160531.1"
                     /translation="MKRLPTRLDGPVLIEPAVFSDSRGFFQETYRRDAYAELGVDVEF
                     VQDNHSRSSRGVVRGMHFQIGSGQAKLVRCARGSILDVVVDIRRDSPTFGQWESVPLD
                     DERLLQLFVPIGFAHGFCVTSDIADVAYKCSSYYDGNLERGFRYDDPDVGIEWPTDLD
                     LHPSQRDVEAPLLREIASELPF"
     gene            2037..>2687
                     /locus_tag="VHF88_01780"
     CDS             2037..>2687
                     /locus_tag="VHF88_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2160532.1"
                     /translation="MPAEPALPPVAILCGGRGTRLQRGTLSIPKPLVEIGGRPIVWHV
                     VEIYAAHGFRDFVLLTGYRGELIEQFVKAAPWPGDVTVRCVDTGVDTPTGGRIHLARD
                     ELGAGTACVTYADGVADIDLRALLAYHRDHGAPATMTVVRPELQFGVTHLDGDGQVTG
                     FEEKPRSEQWINGGFFCLEPGALGYLEDDSVLEREPLRRLAAAGELNAFRHEGFWDC"
BASE COUNT          437 a          881 c          944 g          425 t
ORIGIN      
        1 acccgcgagc cgccgtcgtc gatgcatgac gtcgacttcg ccgacgtggt ggggcgcagg
       61 ccggcggtcc tgctgttcgc cacagcggcg ctctgccaga gccgcgtctg cgggccggtg
      121 atcgacgtca ccgagcaggt caaggccgag cgtggcgacg aggtcgcgtt cgtgcaccag
      181 gagatttacg tcgacaacca gatcgacaaa ggccaacgcc cgtcggtcgc cgcgttcggc
      241 ctcccgaccg agccctggct gttcgccgtc gacgccgacg gccgcatcgc cgcccgcatc
      301 gagggcgcct tcagcgccga cgagctgcat cgcgcgatcg acgccgccgt cggcaagagc
      361 tgatccgctc cgacgacgcg ggacgaccta ggatgagccc tcgaagggcc ggtagctcag
      421 ttggttagag cagcggactc ataatccgta ggtcccaggt tcgagtcctg gccggcccac
      481 tttggttgcc cggcggctag gggctgaggt atagaaccca agaaggacgt gcccgcccgg
      541 ttgaccccgc ttcatgtctt caggtctcgt tcgatcccgg tccgatccac gaggcgcgtc
      601 ggaaacggtc acagcgctag ggcgaggagc ttgaagtcgc ccctttagag gcgttcttct
      661 cgtgggctac agacagagcc agctgccgtc cgggctacga tgtcggggat ggctatctcc
      721 gttcgaaagc tggcgatagg cgagatcgtc gaggttgatg gtcgtcgcta cgaggtcgtg
      781 ccgacgcgcg acggcgagct gacgctcgag acgccgatga cgccgatgga cgagctccac
      841 gccatcgacg ggacgaagcc agcgtcggcc gaggatttcg agcggctgtc tggccacgtc
      901 ccgtacgacg acgagggcta gcccgtgtcg tcagcggggg gaggcggctt gtccgccgag
      961 gtcgtcttcg gcctgcggcc gtgggaggag gaggtcgagc gctaccacca actttcgcct
     1021 ccacgcatca gagcggagaa cgcccgcaag atgatcgagg ccggcgatct caagctcgac
     1081 tggctcaact gcaaacccga cgggccggtt ccgctgccgg gttgtcgcaa gctttacgta
     1141 cctctgggca agcaagcggc ctccgaggcg ccctacgggt ttgtcttcct gctcgtgcag
     1201 cgacgagacg gcacgttggc ctggacgttc gtggccttcg gcgagcggca cccgtccaat
     1261 cccaagaccc gcaccgtcta tgagcgcgcc tataagcgca tcaatggtcg ttatccgagc
     1321 taagaggcag ataaactgac ctgttacctc gcggctggcg ctgtcggcag ctcataagtc
     1381 ggcacgagta caccgacacc gctgcacgaa acgacgggtg cacgtcgtgc gttggcaggc
     1441 cggcgcttag aacggaagct ctgacgcgat ctcgcgcagc agcggcgcct cgacgtcgcg
     1501 ctgcgaggga tggagatcga ggtccgtggg ccactctatg ccgacgtctg ggtcgtcgta
     1561 gcggaagccg cgctcgaggt tgccgtcgta gtaggaggag cacttgtagg cgacgtcggc
     1621 gatgtcgctg gtgacgcaga agccgtgggc gaagccgatc gggacgaaga gctggagcag
     1681 gcgctcgtcg tcgagtggaa ccgactccca ctgtccgaag gtgggcgagt cgcggcggat
     1741 gtcgaccacc acgtcgagga tcgatccgcg cgcgcagcgg acgagcttgg cctggccgct
     1801 gccgatctgg aagtgcatgc cgcgcacgac gccgcgactt gaacgggagt ggttgtcctg
     1861 gacgaactcc acgtcgacgc ccagctcggc gtaggcgtcg cggcggtagg tctcctggaa
     1921 gaagccgcgg ctgtcggaga agacggccgg ttcgatcagc acgggtccgt cgagacgcgt
     1981 gggcagccgc ttcacggcgg cgcaggctat cccaagcgcg tgataatccg gcgccgatgc
     2041 ccgccgagcc cgccctcccg ccggtcgcga tcctctgcgg cggacgcggc acgcgtctgc
     2101 agcgcggcac actgtcgatc ccgaaaccgc tggtcgagat cggcgggcga ccgatcgtct
     2161 ggcacgtcgt cgagatctat gcggcgcacg gcttccgcga cttcgtgctg ttgaccggct
     2221 accgcggcga gctgatcgag cagttcgtga aggccgcccc gtggccgggg gatgtgaccg
     2281 tccgctgcgt cgacaccggc gtcgacacgc cgaccggcgg ccggatccac ctcgctcgcg
     2341 acgagctggg agcgggcacc gcctgcgtca cctacgccga cggcgtcgcc gacatcgacc
     2401 tccgcgcgct gctcgcctac caccgcgacc acggcgcgcc ggcgacgatg acggtcgtcc
     2461 ggccggagct gcagttcggc gtcacgcacc tcgacggcga cggccaggtg acgggcttcg
     2521 aggagaagcc gcgctcggag cagtggatca acggcggctt cttctgcctc gagccgggcg
     2581 ccctcggcta cctcgaggat gacagcgtgc tcgagcggga gccgttgcgg cggctggcgg
     2641 ccgcgggcga gctgaacgcc ttccgccacg aggggttctg ggactgc
//
LOCUS       DATABV010000043         4861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_978043, whole genome shotgun sequence.
ACCESSION   DATABV010000043 DATABV010000000
VERSION     DATABV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978043"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2478)
                     /gene="rpoB"
                     /locus_tag="VHF88_01785"
     CDS             complement(<1..2478)
                     /gene="rpoB"
                     /locus_tag="VHF88_01785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX2160533.1"
                     /translation="MARVDASRLRRSFSRLENALELPNLIDIQRSSFDWLVDTENGGL
                     RETIDDVSPIEDYTGNLAVVFEEIHFDEPVATIAECREKDLTYARPLTVKVAFQNRET
                     GEIREQSVFMGDFPWMTEHGTFIINGTERVVVTQLVRSPGAYVMEPKDREKQVFIANL
                     MPARGSWLELEIDKKGKVFVRIDRKRKLPVSVLLRAMGEVLRESGVAPSWTGSDEDVL
                     ALFDNSLYIRNTLESDTEVTKSEDGALIELFKKQRPGEPPSVDAAKNLLNQLFFDPKR
                     YDLTRVGRYKLNSRLHLDESLDTRTLTPRDIVELIKELVSLPKDLGIPEDGLYEGEPI
                     KDFAAEAQTYGREPIEERLDEYEHFGNRRLRTVGELIQEAFRIGLYRMERVVRERLTT
                     EDSDTITPQTIINIRPVVAAQKEFFGSSQLSQFMDQTNSLSGLTHRRRLSALGAGGLT
                     RERAPIEVRDVHPTHYGRMCPIETPEGPNIGLMGSLSSFAEVSEHGFVTTPYRKVENG
                     VVTDDIVRLDANQEEELVIAQANAEIDQKTGKLKGPQVTCRSRDGELVLAAPKDVDLM
                     DVSPGQIWSVATTLIPFLEHDDANRALMGSNMMRQAVPLLKTEAPLIGTGMEIRAAVD
                     TGDVVIAREAGEVSFVDSERIVVGKDEYPLHKYMRSNQGTIIHQKPLVDVGDKVKAGD
                     VLADGSSTSQGELALGKNCLVAFMSWEGYNFEDAIILSERLVKDDELTSIHIEEYEID
                     ARTTKLGDEEITRDIPNRSEESLRNLDDRGIVRIGAEVVSGDLLVGKVTPKGETELTA
                     EEKLIRAIFKEKAREVRDTSLKVPHGEG"
     gene            complement(2697..3536)
                     /locus_tag="VHF88_01790"
     CDS             complement(2697..3536)
                     /locus_tag="VHF88_01790"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEX2160534.1"
                     /translation="MRRSRTGWDLTKKSWRLLRGQPGLTAYPIVGGLLSLVLFVVPAG
                     LGLYLLDTDSSVLGYVLIAIGVYLASLAATFFGVGLAAAADRAFKGEPVSLSTGVAVA
                     SGRFGAIAGWAFVSAVIGTAISLLQSQRGLAGPILGALGGAAWGLITFLAIPVIAIEG
                     TGPIETIRRSAHLFRERWRGQVTGNVAIGGAVALFGILPSVLLIVLGLALWSNDGGGD
                     AVALGAVLTLIGAAILCVSIVISQALRQVFGIALYRYTADGDVVAGFTEAELASAVKT
                     RGG"
     gene            complement(3604..4248)
                     /locus_tag="VHF88_01795"
     CDS             complement(3604..4248)
                     /locus_tag="VHF88_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160535.1"
                     /translation="MQLAHTLVRGATAPIKLGLSAGELGLSVVRAVVDEARRAVSGGD
                     ASTANGWSGPSEPAVGTPASSTRPHGDPLAPRDRGPSTARTTTATPEAKVASPPAPAP
                     EAVTPPPPVEPVAASGPAVPAVPPTAGAKQVDDDPVPVAEFAEPGAADGAGAEVSIDP
                     PWDGYDGLTAAQIKQRLKDADSEVVATVALYEGLGRKRRSVLDAADRRLRALSS"
     gene            complement(4352..4744)
                     /gene="rplL"
                     /locus_tag="VHF88_01800"
     CDS             complement(4352..4744)
                     /gene="rplL"
                     /locus_tag="VHF88_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX2160536.1"
                     /translation="MATSTEEWIDELKSISVLELSERIKALEEAFGVSAAAVAAAAPA
                     AGAGAAGGDGAAAEEASSTVDVVLTAAGDKKIQVIKGVRAATGLGLKEAKALVDEAPK
                     PIKEGIERDEAEKLKGELEEAGGSAELK"
BASE COUNT          701 a         1647 c         1740 g          773 t
ORIGIN      
        1 gccctcgccg tgcgggacct tgagcgaggt gtcgcggacc tcgcgcgcct tctccttgaa
       61 gatcgcgcgg atcagcttct cctcggcggt cagctcggtc tcgcccttcg gcgtgacctt
      121 gccgacgagc aggtcgccgg agacgacctc ggcgccgatc cgcacgatgc cgcggtcgtc
      181 gaggttgcgc agcgactcct ccgaacggtt cgggatgtcg cgcgtgatct cctcgtcgcc
      241 gagcttcgtc gtgcgagcgt cgatctcgta ctcctcgatg tggatcgagg tgagctcgtc
      301 gtccttgacg aggcgctcgg agaggatgat cgcgtcctcg aagttgtagc cctcccagga
      361 catgaaggcg acgaggcagt tcttgcccag cgccagctcg ccctggctgg tcgaggagcc
      421 gtcagcgagc acgtcgcccg ccttgacctt gtcgccgacg tcgacgagcg gcttctggtg
      481 gatgatcgtg ccctggttgc tgcgcatgta cttgtgcagc gggtactcgt ccttgccgac
      541 gacgatccgc tcggagtcga cgaacgacac ctcgccggcc tcgcgggcga tgacgacgtc
      601 gccggtgtcg acggcggcgc ggatctccat gccggtgccg atcagcggcg cctccgtctt
      661 gagcagcggc accgcctggc gcatcatgtt cgagcccatc agcgcgcggt tggcgtcgtc
      721 gtgctcgagg aacgggatca gcgtcgtggc gaccgaccag atctggccgg gcgagacgtc
      781 catcaggtcg acgtccttcg gcgcggcgag caccagctcg ccgtcgcgcg agcggcaggt
      841 gacctgcggg cccttcagct tgccggtctt ctggtcgatc tccgcgttcg cctgggcgat
      901 gaccagctcc tcctcctggt tcgcgtcgag gcgcacgatg tcgtcggtga cgaccccgtt
      961 ctcgaccttg cgataggggg tggtgacgaa gccgtgctcg gagacctcgg cgaacgagga
     1021 cagcgagccc atcaggccga tgttcgggcc ctcgggggtc tcgatcgggc acatccggcc
     1081 gtagtgggtc ggatggacgt cgcgaacctc gatcggggcg cgctcgcgcg tcagtccgcc
     1141 ggcgccgagc gccgacaggc ggcgacggtg cgtcaggccc gagagcgagt tcgtctggtc
     1201 catgaactgc gacagctgcg aggagccgaa gaactccttc tgggcggcga cgaccgggcg
     1261 gatgttgatg atcgtctgcg gcgtgatcgt gtccgagtcc tcggtcgtga gccgctcgcg
     1321 gacgacgcgc tccatccggt agaggccgat ccggaaggcc tcctggatca gctcgccgac
     1381 ggtccgcagg cggcggttgc cgaagtgctc gtactcgtcg aggcgctcct cgatcggctc
     1441 gcgcccgtag gtctgggcct cggcggcgaa gtccttgatc ggctcgccct cgtagagacc
     1501 gtcctcgggg atgccgaggt ccttcggcag cgagacgagc tccttgatca gctcgacgat
     1561 gtcgcgcggg gtcagcgtac gcgtgtcgag cgactcgtcg aggtgcaggc gcgagttgag
     1621 cttgtagcgg ccgacgcgcg tcaggtcgta gcgcttcggg tcgaagaaga gctggttgag
     1681 caggttcttg gcggcgtcga ccgacggcgg ctcgcccgga cgctgcttct tgaacagctc
     1741 gatcagcgcg ccgtcctccg acttcgtgac ctcggtgtcg gactcgagcg tgttgcggat
     1801 gtagagcgag ttgtcgaaca gcgcaaggac gtcctcgtcg ctgccggtcc agctcggcgc
     1861 gacgccgctc tcgcgcagga cctcgcccat cgcacgcagc agcacgctga ccggcagctt
     1921 gcgcttgcgg tcgattcgaa cgaagacctt gcccttcttg tcgatctcga gctcgagcca
     1981 ggagccgcgc gccggcatca ggttcgcgat gaagacctgc ttctcgcggt ccttgggctc
     2041 catcacatag gcgcccggcg agcgcaccag ctgcgtcacg acgacgcgct cggtgccgtt
     2101 gatgatgaag gtgccgtgct cggtcatcca cgggaagtcg cccatgaaga cggactgctc
     2161 gcggatctcg ccggtctcgc ggttctggaa cgcgaccttg acggtcagcg ggcgggcgta
     2221 ggtgaggtcc ttctcgcgac actcggcgat cgtcgcgacg ggctcgtcga agtggatctc
     2281 ctcgaagacg acggcgaggt tgccggtgta gtcctcgatc ggcgagacgt cgtcgatggt
     2341 ctcgcgaaga ccgccgttct cggtgtcgac cagccagtcg aaggagcttc gctggatgtc
     2401 gatgaggttc ggtagctcga gggcgttctc gaggcgtgag aaactgcggc gcaggcgaga
     2461 ggcatcaaca cgtgccaaga cggcggctcc ttcgaggttc ggcgggagag cgagacgggc
     2521 gcgaaaagcg cgaccgtcca agatagcaca ctgaagaccg ccccctggag gggcggttgc
     2581 cgcaccccct gaggggtgcg gacacggcca tcacggaccc cgtcccgagg ctccgcgata
     2641 tgtgggcgaa gcgcaactcg agggacggct acggctatag gccagacttc agggtatcag
     2701 ccaccgcggg tcttcacggc cgatgcaagc tcggcctcgg tgaacccggc gacgacgtcg
     2761 ccgtcggcgg tgtagcggta gagcgcgatg ccgaagacct ggcgcagcgc ctgagatatg
     2821 acgatgctga cgcagaggat cgcggcgccg atcagcgtga gcaccgcgcc gagcgcgacc
     2881 gcgtcgccgc cgccgtcgtt gctccacagc gcgaggccga ggacgatcag caggaccgac
     2941 ggcaggatgc cgaagagcgc gactgcgccg ccgatcgcga cgttgccggt gacctggccg
     3001 cgccagcgct cgcggaagag atgcgccgag cggcggatcg tctcgattgg ccccgtcccc
     3061 tcgatcgcga tcacgggaat cgcgagaaag gtgatcaggc cccaggccgc gccgccgagc
     3121 gcgccgagga tcgggccggc caagccgcgc tggctctgga gcagcgagat cgccgtgccg
     3181 atgaccgccg agacgaacgc ccagcccgcg atcgcgccga agcggccgct cgcgaccgcc
     3241 acgccggtgc tcagcgacac cggctcgccc ttgaaggccc ggtcggccgc tgccgccaga
     3301 ccgacgccga agaacgtcgc cgccagtgac gcgagataga cgccgatcgc gatcagcacg
     3361 tagcccagca cgctcgagtc ggtgtcgagc aggtagagcc ccagtcccgc tgggacgacg
     3421 aagaggacga gcgagagcag gccgccgacg atcggatagg cggtcaggcc gggctgaccg
     3481 cgcagcagtc gccagctctt cttggtcagg tcccagccgg ttcgagagcg tcgcatgctg
     3541 ccaaggttcg gcagcacggc tgcggatcct cagtcgtgca agcggtacgc caacggttgg
     3601 acgtcaggag ctgagcgcgc gcaggcggcg atccgccgcg tcgagcaccg agcggcgctt
     3661 gcgaccgagg ccctcgtaga gcgcgacggt cgccacgacc tcgctgtcgg cgtccttgag
     3721 ccgctgcttg atctgggccg cggtcagccc gtcgtagccg tcccagggcg gatcgatgct
     3781 gacctcggcg ccggccccgt cggcggcacc gggctcggcg aactcggcca ccggcaccgg
     3841 atcgtcgtcg acctgcttcg cgccggccgt cggcggcacc gcggggaccg ccgggccgga
     3901 ggccgccacg ggctcaacgg gcggtggcgg ggtgaccgcc tcgggcgccg gagccggcgg
     3961 ggacgccacc ttggcctcgg gcgtagcggt agtggtgcga gcggtcgagg gaccgcgatc
     4021 gcgcggggcg agcggatcgc cgtgcgggcg ggtgctggac gccggcgtcc cgaccgccgg
     4081 ctccgacggg cccgaccagc cgttggcggt ggacgcgtcc ccgccggaga ccgcgcggcg
     4141 cgcctcgtcg acgactgccc ggacgaccga gagccccagc tcgccggccg acaggccgag
     4201 cttgatcggc gccgtggcgc cgcggaccag tgtgtgagcg agctgcatcg ccgatccgcc
     4261 tacccggggt cagatggcgt gaaacgccgg atgagcacga aggggccgcg cagtggcggc
     4321 cccttcggtg aaacctgctg tcgcggtcga gctacttgag ctcggcggag ccgccagcct
     4381 cctcgagctc gcccttgagc ttctcggcct cgtcgcgctc gatgccctcc ttgatcggct
     4441 tcggcgcctc gtcgacgagc gccttggcct ccttgaggcc aaggccggta gcggcacgga
     4501 cgcccttgat cacctggatc ttcttgtcgc cggcggcggt caggacgacg tcgacggtgc
     4561 tcgacgcctc ctcggccgcg gcgccgtccc cgcccgctgc gccggccccg gcggcggggg
     4621 ccgccgcggc gacggccgcg gcggagacgc cgaacgcctc ctcgagcgcc ttgatccgct
     4681 cggagagctc gagcaccgaa atgctcttca gctcgtcgat ccactcctca gtgctggtgg
     4741 ccatttcagc cctccttctc ttgatcgccc tcggagggct cgtcggtttg gtcggcgtcc
     4801 gccgctgacg actcagcggg cgcctcgtct gtctactcgg cgtcggcgac tggcgcctcg
     4861 g
//
LOCUS       DATABV010000044         6182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2102664, whole genome shotgun sequence.
ACCESSION   DATABV010000044 DATABV010000000
VERSION     DATABV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102664"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..981
                     /gene="mreC"
                     /locus_tag="VHF88_01805"
     CDS             163..981
                     /gene="mreC"
                     /locus_tag="VHF88_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX2160537.1"
                     /translation="MLTVYFGEGGGGVFHTMQRGAQEAFAPLETGTGRALKPFRDLLG
                     WFGDTLDAKGENEELRAEVERLRRQVGVAETARHDVRELRGIVGLTESEGYPQGTEPV
                     GARVIQRSPTVWYSTIKIDKGSGDGVRRDQPVVAAGGLVGVVSDVTGGTAEVTLITDS
                     SSAVSAQVMPNGATGIVHPVVGDPDDLLLDYIADGRRVTEGTTVVTSGFESDGVESLF
                     PRGIAIGRVTKVDPEEVEQFQRVHLEPFADIRGIDYVQVLTERGVSTQAGVPLQ"
     gene            978..1556
                     /gene="mreD"
                     /locus_tag="VHF88_01810"
     CDS             978..1556
                     /gene="mreD"
                     /locus_tag="VHF88_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX2160538.1"
                     /translation="MSSTPGAYVRCGALALVAVILQTSGLGSGRVLGAYVDLVPLVVA
                     AVAIYAGAIPGAAMGFATGLVLDLLVGQTMGASSLVLTAVGYGVGRYREVRDPAHGLM
                     PIPVGAVATFGWLLAYAAVSFMLDVGATVSWLVVREMVATTLIGAVLALPVFAVCRRV
                     LRPVLLVDPLDVRRRRRAPREAGPIGLRGLEV"
     gene            1558..3417
                     /gene="mrdA"
                     /locus_tag="VHF88_01815"
     CDS             1558..3417
                     /gene="mrdA"
                     /locus_tag="VHF88_01815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2160539.1"
                     /translation="MYLDPGDRPSLTPQLAVRVAILGGIALLAFALVFFRLWYLQVLS
                     GDTYRAEANDNRVRAIKVRAPRGEIVDREGRVLVENRVALAVKVSPKQLPENPAQRSA
                     VYRRLGEVLDMRPRGIERRVEKQLEELPYATATVKQDVDENIVAYLMERSADFPGVQP
                     ERVFVREYPQGETGAHLFGYTGEVSQEELDGIYSDVEAGDLVGKGGIEQQYDRFLRGR
                     NGATRVQVDALGTVRGEPLAAEPPEQGRQLRLALDLDVQEAGQAALAGRPGGFVAMDV
                     RTGEVVALGSGPTFDPNFFSKPFGQEAFDRLNSEAAGKPLLNRAIQAEYPAGSTFKLV
                     TAAAALEGGLITPQDVIVDNGSVRIGDIDFINAGEVPHGPVALRQALSVSSDVYFYLL
                     GQKAETTGDGLLIQRWARKLGIGRPTGIDLPHEQVGRVPTPQWRKEWVEQGLLDRPWS
                     VGDNVNFSIGQGDLLTNPLQMAIAYAAVANGGKVVRPHLGLRIEDSSGRPLQELASEP
                     ARRVKIAPEHRQAILDGIYSAANDPGGTSTPVFETFPVEIAGKTGTAETSQGDQSWYV
                     ALAPYPNPRYVVAATVEGGGFGAETAAPAVRQILTALLDVEDDGEILAGTAVD"
     gene            3441..4628
                     /gene="rodA"
                     /locus_tag="VHF88_01820"
     CDS             3441..4628
                     /gene="rodA"
                     /locus_tag="VHF88_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX2160540.1"
                     /translation="MHATPLGATVREFRERSGPSFVRIDALLLLAVLGLTVCSLYTIG
                     EATRDEVAGSPHYFLHRQAAFAAIGIVLMLGIARIDYTRLREWKLQMYAALIGGVLLV
                     IVLGSIANGSGRALELGFFELQFSELGKVLLILTLAAVIVDRVRQIGDRRTTAAVMLL
                     ALIPALLVISQDLGTGMVYITIAVVILFVAGTPWPHFAALGALAATAATIVLIGAPAV
                     GIELLQPYQEDRLTAFLNPSDNPGDEGYQQLQSQVAIGAGGKTGRGDQATQTQLEFLP
                     EEHTDFIFSVVGEEFGFMGAALVLSLFALLIWRSLRILTMSKDLFGTLIAAGVLAMLM
                     HQIFINVGMTIGIMPITGITLPLMSYGGSSVIATFIALGLLQSVHAQARETAFAKGRD
                     LAI"
     gene            4641..>6182
                     /locus_tag="VHF88_01825"
     CDS             4641..>6182
                     /locus_tag="VHF88_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX2160541.1"
                     /translation="MKKQVLVTADRGETRVAILEAEGALSAEDGKPKGGAGRSQQKQD
                     DWKVAELYVERRGSRSIVGNVYKGRVDNVLPGLEAAFVDIGLDKNGFLHADDIVMEGV
                     EVARRGRGSGGKGRQISDLLKPGQEIVVQAIKDPLKSKGSRLSMQLSIAGRYLVYTPQ
                     GEGVGVSRRLEDKERDRVRKEAKGLEIGEGGVIVRTAAQGAKRADFEHEIKYLHKLHE
                     VLQQRAEKAKAPAMIFQEADLSVRVVRDIFSKQFDRAIVDDEQQHHRLESFFNRTAPE
                     LLPRLELYKDRKTPLFERYKIDQEIQSTLHRRVDLRSGGYLIIDYTEALTVIDVNSGS
                     FVGRGKGARLEDTITKTNLEAAEEAVRQLRLRDIGGIIVIDFIDMARARNRDAVLKTL
                     RKSLDEDRTKTYVVEISPLGLVEMTRQNVTEGVREILTKTCPTCEGEGVVLSEESVSI
                     DAERRLRELAAERPEPEAYLIQIHPRVAALLAGGVGRPLLQIEEETGKSFHFEGSEAL
                     PLDHFA"
BASE COUNT          925 a         2143 c         2187 g          927 t
ORIGIN      
        1 gctgacgtcg cagccgctcg acctcggcgc ggagctgctc gttctcgccc ttcgcgtcga
       61 gcgtgtcgcc gaaccagctg aagaggtcgc ggaacggctt tagcgcacgg ctggcgccgg
      121 tctcgagcgg cgctcgcgat attcgtcgcg ctctcgatcg gcatgctgac cgtctacttc
      181 ggcgagggcg gcggcggggt gttccacacg atgcaacgcg gcgcgcagga ggcgttcgcg
      241 ccgctcgaga ccggcacggg gcgggccctc aagccgtttc gcgacctgtt aggctggttc
      301 ggcgacacgc tcgacgcgaa gggcgagaac gaggagttgc gcgccgaggt ggagcggctg
      361 cggcgccagg tcggcgtcgc cgagaccgcc cgccacgacg tgcgcgagct gcgcgggatc
      421 gtcggattga ccgagagcga gggctacccg caggggacag agcctgtcgg tgcgcgggtg
      481 atccagcgct cgccgaccgt ctggtactcg acgatcaaga tcgacaaggg gtcgggcgac
      541 ggcgttcgtc gcgatcagcc ggtcgtcgcc gccggcgggc tcgtcggggt ggtcagcgac
      601 gtcaccggcg ggacggccga ggtcacgctg atcaccgact cctcgagcgc ggtgtcggcc
      661 caggtgatgc ccaacggcgc gaccgggatc gtccatccgg tcgtcggcga tccggacgac
      721 ctgctgctcg actacatcgc cgacggccgg cgcgtgacgg aggggacgac ggtcgtgacg
      781 agcgggttcg aatcggacgg cgtcgagtcg ctgttcccgc gcggcatcgc gatcgggcgc
      841 gtgaccaagg tcgatccgga ggaggtcgag cagttccagc gcgtccacct cgagccgttc
      901 gccgacatcc gcggcatcga ctacgttcag gtcctgaccg agcgcggcgt cagcacgcag
      961 gcgggggtcc cgctccagtg agctcgacgc ccggcgccta cgtccgctgc ggagcgctcg
     1021 cgctcgtcgc ggtgatcctg cagacctcgg gcctcggcag cggccgcgtg ctcggggcct
     1081 acgtcgacct cgtcccgctc gtcgtcgcgg ccgtcgcgat ctacgccggg gcgatcccgg
     1141 gcgccgcaat ggggttcgcg accgggctgg tgctcgacct gctcgtcggt cagacgatgg
     1201 gggcgtcgtc gctggtgctg accgcggtcg gctacggcgt cggccgctac cgcgaggttc
     1261 gcgaccccgc gcacgggttg atgccgatcc cggtcggggc ggtcgcgacg ttcggctggc
     1321 tgctcgccta cgcggccgtg tcgttcatgc tcgacgtcgg cgcgacggtc agctggctgg
     1381 tcgtccgcga gatggtcgcg acgacgctga tcggcgcggt cctcgcgctg ccggtgttcg
     1441 cggtctgccg tcgcgtgctg cgcccggtcc tgctcgtcga cccgctcgac gtgcgccgtc
     1501 gccggcgcgc cccgcgcgag gcgggtccga tcggcctgcg cgggctggag gtctgaggtg
     1561 tacctcgacc cgggcgatcg tccctcgctg acaccgcagc tcgcggtccg cgtggcgatc
     1621 ctcggcggga tcgcgctgct ggcgttcgcg ctcgtcttct tccggctctg gtacctgcag
     1681 gtcctgtccg gcgacaccta ccgcgccgag gccaacgaca accgcgtgcg ggcgatcaag
     1741 gtccgtgcgc cgcgcggcga gatcgtggac cgcgagggcc gcgtgctggt cgagaaccgc
     1801 gtcgcgctcg cggtgaaggt ctcgccgaag cagcttccgg agaaccccgc gcagcgcagc
     1861 gccgtctacc gccgcctcgg cgaggtgctc gacatgcgcc ctcgcgggat cgagcggcgt
     1921 gtcgagaagc agctcgagga gctgccctac gcgacggcga cggtcaaaca ggacgtcgac
     1981 gagaacatcg tcgcctacct gatggagcgc agcgccgact tcccgggcgt ccagccggag
     2041 cgggtcttcg tgcgcgagta cccgcagggg gagaccggcg cgcacctgtt cggctacacc
     2101 ggcgaggtca gtcaggagga gctcgacggc atctacagcg acgtcgaggc cggcgacctg
     2161 gtcggcaagg gcggcatcga gcagcagtac gaccgcttcc tgcgcggccg caacggcgcg
     2221 acgcgagtcc aggtcgacgc gctcggcacg gtccgcggcg agccgctggc ggccgagccg
     2281 ccggagcagg gccgccagct gcggctggcg ctcgacctcg acgtccagga ggccggtcaa
     2341 gccgcgctcg cgggtcggcc gggcggcttc gtcgcaatgg acgtccgcac cggcgaggtg
     2401 gttgcgctcg gcagcggtcc gacgttcgac ccgaacttct tctcgaagcc gttcggtcag
     2461 gaggccttcg accggctcaa ctcggaggcg gccggcaagc cgctgctcaa ccgcgcgatc
     2521 caggccgagt atccggcggg gtcgacgttc aagctcgtca ccgcggccgc tgcgctcgag
     2581 ggcggtctga tcaccccgca ggacgtgatc gtcgacaacg gctcggtgcg gatcggcgac
     2641 atcgacttca tcaacgccgg cgaggtcccg cacgggccgg tcgcgctgcg ccaggcgctg
     2701 tcggtgtcga gcgacgtcta cttctacctg ctcggccaga aggccgagac gacgggcgat
     2761 ggactgctga tccagcgctg ggcacgcaag ctcgggatcg gacgcccgac ggggatcgac
     2821 ctgccgcacg agcaggtcgg gcgcgtcccc acgccgcagt ggcgcaagga gtgggtcgag
     2881 cagggtctcc tcgaccgtcc ctggtcggtc ggcgacaacg tcaacttctc gatcgggcag
     2941 ggagacctgc tgaccaaccc gctccagatg gcgatcgcct acgccgcggt cgccaacggc
     3001 ggcaaggtcg tcaggcccca tctcgggctg cggatcgagg actcgtccgg acggccgctg
     3061 caggagctcg cctccgagcc ggcgcgtcgg gtgaagatcg cgcccgagca ccgtcaggcg
     3121 atcctcgacg gcatctactc ggccgccaac gatcccggcg gcacctcgac ccccgtcttc
     3181 gagaccttcc cggtcgagat cgccggcaag accggcaccg ccgagaccag ccagggcgac
     3241 cagtcgtggt acgtcgcgct cgcgccgtac ccgaacccgc gctacgtcgt cgcagcgacc
     3301 gtcgagggcg gcggcttcgg cgccgagacc gcggcgccgg ccgtgcggca gatcctgacc
     3361 gcgctgctcg acgtcgagga cgacggcgag atcctcgccg gaacggcggt cgactaggcg
     3421 atggataggg tggcccactg atgcatgcga cccctctcgg cgcgaccgtg cgcgagttcc
     3481 gcgagcggtc gggcccgagc ttcgtgcgga tcgacgcgct gctgctgctc gccgtcctcg
     3541 ggcttaccgt ctgctcgctc tacacgatcg gcgaggcgac gcgcgacgag gtcgcgggca
     3601 gcccgcacta cttcctccac cgccaggccg cgttcgcggc gattggcatc gtcctgatgc
     3661 tgggcatcgc gcggatcgac tacacgcgcc tgcgtgagtg gaagctgcag atgtacgccg
     3721 cgctgatcgg tggcgtgctg ctcgtcatcg tgctcggatc gatcgcgaac ggctctggcc
     3781 gcgcgctcga gctcgggttc ttcgagctgc agttctcaga gctcggcaag gtcctgctga
     3841 tcctgacgct cgccgcggtg atcgtcgacc gggtgcgtca gatcggcgac cgccggacga
     3901 ccgcggcagt gatgctgctc gcgctgatcc cggcgctgct ggtgatctcg caggacctcg
     3961 gcaccggcat ggtctacatc acgatcgcag tggtgatcct gttcgtcgcg ggcacgccgt
     4021 ggccgcactt cgcggcactt ggcgcactcg ccgcgacggc cgcgacgatc gtcctgatcg
     4081 gcgcgccggc ggtcggcatc gagctgctcc agccgtacca ggaggaccgc ctgacggcct
     4141 tcctcaaccc ttccgacaac cccggcgacg agggctacca gcagcttcag tcacaggtgg
     4201 cgatcggcgc gggcggcaag accggccgcg gcgatcaggc gacccagacc cagctcgagt
     4261 tcctgccgga ggagcacacc gacttcatct tctccgtcgt cggagaggag ttcggcttca
     4321 tgggggcggc actagtgctg tccctgttcg cactgctgat ctggcgctcg ctgcgcatac
     4381 tgacgatgtc caaggatctg ttcggcacgc tgatcgcggc cggcgtcctt gcaatgctta
     4441 tgcaccagat cttcataaac gtgggcatga ccatcgggat catgcccatc accggcatca
     4501 cgctgccgct catgagctat ggcggctcgt cggtgattgc aaccttcatc gctctcgggc
     4561 ttctgcagag cgtccacgcg caagcgcgcg agacggcgtt cgcgaagggc cgggacctag
     4621 ccatatgaga agaggaccct ttgaaaaagc aagtactcgt cacagccgac cgcggcgaga
     4681 cacgagtcgc catcctcgag gcggagggcg cgctctccgc tgaggacggc aagcccaagg
     4741 gcggcgcagg ccgctcccag cagaagcagg acgactggaa ggtcgccgag ctctacgttg
     4801 agcggcgcgg gtcgcgctcg atcgtcggca acgtctacaa gggccgcgtc gacaacgtgc
     4861 tgcccgggct cgaggcggcc ttcgtcgaca tcgggctcga caagaacggc ttcctgcacg
     4921 ccgacgacat agtcatggag ggcgtcgagg tcgcgcgccg cggccgcggc agcggcggga
     4981 aggggcgtca gatctccgac ctgctcaagc cgggccagga gatcgtcgtc caggcgatca
     5041 aggacccgct caagtcgaag ggctcgcgcc tctcgatgca gctgtcgatc gctggccgct
     5101 acctcgtcta cacgccccag ggcgagggcg tcggcgtctc gcgccgactc gaggacaagg
     5161 agcgcgaccg cgtgcgcaag gaggccaagg gcctcgagat cggcgagggc ggcgtgatcg
     5221 tccggaccgc cgcgcagggc gccaagcggg ccgacttcga gcacgagatc aagtacctgc
     5281 acaagctcca cgaggtgctc cagcagcgcg ccgagaaggc gaaggcgccg gcgatgatct
     5341 tccaggaggc cgacctctcg gtccgcgtgg tgcgcgacat cttctcgaag cagttcgacc
     5401 gcgcgatcgt cgacgacgag cagcagcacc accggctcga gtccttcttc aaccgcaccg
     5461 cgccggagct gctgccgcga ctggagctct acaaggaccg caagacgccg ctcttcgagc
     5521 gctacaagat cgatcaggag atccagtcga cgctgcaccg ccgcgtcgac ctgcgctccg
     5581 gcggctacct gatcatcgac tacaccgagg cgctgaccgt catcgacgtc aactccggct
     5641 cgttcgtcgg acgcggcaag ggcgcgcggc tcgaggacac gatcaccaag accaacctcg
     5701 aggccgccga ggaggccgtt cgccagctgc ggctgcgcga catcggcggg atcatcgtca
     5761 tcgacttcat cgacatggcg cgcgcgcgca accgcgacgc ggtgctcaag acgctgcgca
     5821 agtcgctcga cgaggaccgc acgaagacgt acgtcgtcga gatctcgccg ctcgggctgg
     5881 tcgagatgac gcgccagaac gtcaccgagg gcgtccgcga gatcctcacc aagacctgtc
     5941 cgacctgtga gggcgagggc gtcgtgctgt ccgaggagtc ggtctcgatc gacgccgagc
     6001 gccggctgcg cgagctcgcc gccgagcggc ccgagcccga ggcctacctg atccagatcc
     6061 atccgcgcgt tgccgcgctg ctcgccggcg gcgtcggccg gccgctgctg cagatcgagg
     6121 aggagaccgg caagagcttc cacttcgagg gctccgaggc gctgccgctc gaccacttcg
     6181 cg
//
LOCUS       DATABV010000045         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2585523, whole genome shotgun sequence.
ACCESSION   DATABV010000045 DATABV010000000
VERSION     DATABV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585523"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..87
                     /locus_tag="VHF88_01830"
     tRNA            14..87
                     /locus_tag="VHF88_01830"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Pro,seq:cgg)
     gene            95..553
                     /locus_tag="VHF88_01835"
     CDS             95..553
                     /locus_tag="VHF88_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2160542.1"
                     /translation="MPSDRFYTRLSMAAPASALKATGKLNIPIYRLTGGRVFGSLDGN
                     PILLLTTTGRKSGQPRTAPVIYMPDGDRMVVIGTNAGNLRTPAWALNLEACPDAEVEV
                     RRRRLPVRGRVATGDERAELWRRFSATYSGFDDYAANVARDIRLFVLEPR"
     gene            complement(550..1065)
                     /locus_tag="VHF88_01840"
     CDS             complement(550..1065)
                     /locus_tag="VHF88_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160543.1"
                     /translation="MERSQQQRASEQEERNRGEARDMLRDELREVAGDEGVERAERKG
                     ALDEAIDGRSPKTVTGVLSESRGIIAITFFVALIVGALIALITGAWWFIIVALVLHAI
                     GTLVVVGTAFSLATRGESPDPRTAAALEARGVRDPDAALDQVVEAATDGRVGRDGGET
                     TAGEGDSGETR"
     gene            complement(1154..>1600)
                     /locus_tag="VHF88_01845"
     CDS             complement(1154..>1600)
                     /locus_tag="VHF88_01845"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX2160544.1"
                     /translation="DMVANVHAEASGRLLATRLWEMTDDPGMKDMLSFLIARDTMHQN
                     QWMAVLEELKDMGMPAPSDFPQEQENQEHSYEFFVHSDGEVPGNARWTSGPSVDGKGS
                     FSVREAEPLGGPPPELAPPESRPELHNTIEQAKGGFMRKAKDAITP"
BASE COUNT          208 a          598 c          549 g          245 t
ORIGIN      
        1 cttcgagcgg tcacgggctg tggcgcagtc tggtagcgcg ctccgttcgg gtcggagagg
       61 tcccgagttc aaatctcggc agcccgattt cgccatgccc agcgaccgct tctacacccg
      121 cctgtcgatg gcggcgccgg ccagcgcgct gaaggcgacg gggaagctca acatcccgat
      181 ctaccgcctg accggagggc gggtgttcgg cagcctcgac ggcaacccga tcctgctgct
      241 gacgacgacc ggacgcaagt cgggccagcc gcggaccgcg cccgtgatct acatgcccga
      301 cggcgaccgg atggtcgtca tcgggaccaa cgccggcaac ctgcgcacgc cggcgtgggc
      361 gctcaacctc gaggcctgcc ccgacgccga ggtcgaggtc cggcgcaggc ggctgccggt
      421 gcgcggccgc gtcgcgaccg gcgacgagcg cgccgagctg tggcgccgct tcagcgcgac
      481 ctactccggc ttcgacgact acgccgcgaa cgtcgcgcgc gacatccgcc tgttcgtgct
      541 cgagccgcgc tagcgggtct cgccggagtc gccctcgccg gcggtcgtct cgccgccgtc
      601 gcggccgacg cggccgtcgg tcgcggcctc gacgacctga tcgagcgcgg cgtcggggtc
      661 gcgcacgccg cgcgcctcga gcgccgccgc ggtgcgcggg tccggactct cgccgcgcgt
      721 ggcgagcgag aaggccgttc cgacgacgac cagtgtgccg atcgcgtgca gcacgagtgc
      781 gacgatgatg aaccaccagg ccccggtgat cagcgcgatc agcgcaccca cgatcagcgc
      841 gacgaagaag gtgatcgcga tgatcccgcg cgactcgctg agcacgccgg tgaccgtctt
      901 cggcgaccgt ccgtcgatcg cctcgtcgag cgcccccttg cgctccgcgc gctcgacccc
      961 ctcgtcgccg gcgacctcgc gcagctcgtc gcgcagcatg tcgcgcgcct ccccgcggtt
     1021 gcgctcctcc tgctcgctcg cacgctgttg ctgactccgc tccatggttg cggcctaccc
     1081 ccgccacaag gctcgaaacc acccgcccgc gccggagtga tgccggacgc gggcgagtcg
     1141 gctcggggag cgatcagggc gtgatcgcgt ccttggcctt gcgcatgaag ccgcccttcg
     1201 cctgctcgat cgtgttgtgc agctcggggc gcgactccgg cggcgcgagc tcgggcggcg
     1261 ggccgccgag cggctcggcc tcgcggaccg agaacgagcc ctttccgtct accgacgggc
     1321 cgctggtcca gcgcgcgttg cccggcacct caccgtcaga gtggacgaag aactcgtagc
     1381 tgtgctcctg gttctcctgc tcctgcggga agtcgctcgg cgccggcatg cccatgtcct
     1441 tcagctcctc gagcaccgcc atccactggt tctggtgcat cgtgtcgcgc gcgatcagga
     1501 aggacagcat gtccttcatt ccggggtcgt cggtcatctc ccagagacgc gtggcgagca
     1561 gccggccgct ggcctcggcg tggacgttgg cgaccatgtc
//
LOCUS       DATABV010000046        19071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_416540, whole genome shotgun sequence.
ACCESSION   DATABV010000046 DATABV010000000
VERSION     DATABV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 19071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19071
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416540"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VHF88_01850"
     CDS             <1..874
                     /locus_tag="VHF88_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2160545.1"
                     /translation="IAASGAAGASGAVAGDRAGGTGAAPVGSSFEPSSPAPIELLGVD
                     AFDVKWRMPPRAALVFDVENGDSLYRRRTTHELPIASLTKIMTALAVTAATEPGEVVR
                     VPKALRYEGSAVGLLPLGRRVRIEPLLAGLMIVSGNDAALALADHVSGSERRFVTAMN
                     EQARLWDLDCTHFATPHGLSDADRSCAVDLAVMTRLAMRVPRIVDLSRRAQAAYRFPI
                     KGRKLYLSGHNPLIRAGYPGAIGLKTGYTDAAGRCFVGVARRHGRTFGVVLLDSPDPG
                     RQAARLLDLAFEFG"
     gene            complement(947..2011)
                     /gene="ligD"
                     /locus_tag="VHF88_01855"
     CDS             complement(947..2011)
                     /gene="ligD"
                     /locus_tag="VHF88_01855"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2160546.1"
                     /translation="MAKAEATMLEAGGRELRVTSADRVIFPATERTAEITKLQIVEYY
                     LAVEDGIMRALRERPTTLERWPKGVHEGMTLTTREGQKGDAFYQKRVPKGAPDYVETA
                     RIEFPSGRSADEVCPTEIATVGWCAQMGTLTFHPWPVRRADVDHPDELRLDLDPQPWT
                     DFDDAVRVAGVARELLGELGYAGFPKTSGGRGIHIYVRIEPRWTFTDVRHAAIAFGRE
                     LERRLPGEVTTKWWKEERGEKIFVDYNQNARDRTIASPYSVRPKPGAPVSAPLSWDEL
                     TEVAPEDFTVATMPARFAEVGDRFATIDDVAHSLEPLLEMYERDEAEGAGDMPYPPDY
                     PKMPGEPKRVQPSRDTDRKK"
     gene            2084..2156
                     /locus_tag="VHF88_01860"
     tRNA            2084..2156
                     /locus_tag="VHF88_01860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2117..2119,aa:Ala,seq:tgc)
     gene            2269..2847
                     /locus_tag="VHF88_01865"
     CDS             2269..2847
                     /locus_tag="VHF88_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160547.1"
                     /translation="MKQSLSSAGRLARYGGATAGVLAAALIGGPTVLAQDGDTPAEPT
                     TITMEAQGKRLVFDGPETVQKGDELRIVNDTSVRKYGPHTFSLVKQSLLPDSGKEFRQ
                     CFTPGKICMEIAMAHKFNPRTEKVGKPVVNAGKRGWDRSFNGKRNGDSWYTDKKDQSF
                     TRRVTAKAGTTLSFICAVHPDMQGEIDVVDAE"
     gene            2900..4480
                     /locus_tag="VHF88_01870"
     CDS             2900..4480
                     /locus_tag="VHF88_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX2160548.1"
                     /translation="MKAIQDTAERGLSRRALLAGAAAGAITLGLPGRGRTSVHPPVRL
                     RPFAQPLRIPEVLTGPELAIPMRHAEVDVLPGRPTRMWTYDGSFPGPTVRRPAGETTR
                     VRFDHRLGERAGELTVHLHGGHNRASEDGQPGGLTARQPRSLYCDISPGLTAAESGND
                     LLIAPGGSRTYSYEQVEDGQPERASFQWYHDHRLDRTARNVWRGLAGMWIVDDELDAS
                     LPLPRGRRDIPLMLADRSFDRRNQLTDPFAGGGLRPPDDGVVGRHILVNGVVLPHHDV
                     RPQRYRLRVLNASQFRSYNVCLTRGVKIVQIATEGGLLPRPLRRRAVLLGPGERAELI
                     VDFAQARGERVQLVSVPRRGAPHKLGSQSYAGPLMEFRVGSRRLRDTTAIPAALRPLP
                     AWIADAPSKPSHSWKLTVGLGLRPSWLINGRTYDPGYVETSAALGEVVTWELHNRTAA
                     AHLMHLHHTDWYMLSRNGRRPPPWERCLKDTFFLDPGERVVIAGKLSDYPGRYVVHCH
                     MLDHEDHGLMSQFEVTAA"
     gene            complement(4490..4984)
                     /locus_tag="VHF88_01875"
     CDS             complement(4490..4984)
                     /locus_tag="VHF88_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160549.1"
                     /translation="MLGPFRDRSGRLRLWPVALLLWTALVVGMVWNAAPDVPPAVASP
                     ERELLGELPPGYRYELQPDTDPATDDWHTATLLVLRGCMRAATVTVIGRDGGAHVSFT
                     RGQIEHDSGARLSSTDLVRGDAFAGWLSDREWVLVAQRGEVVVSIHMPPYSEGRRLSR
                     AILN"
     gene            complement(5074..6183)
                     /locus_tag="VHF88_01880"
     CDS             complement(5074..6183)
                     /locus_tag="VHF88_01880"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2160550.1"
                     /translation="MAAAIAVPAQAAAADPPGTAQVLDATLDRLAATPAGPPGVSVLI
                     QRGERREYRRRGVGDLARDTPPGVHDHVRIASVSKAFSGAVALALVDRGELELRDTVG
                     ELLPGLLPRARRVTLRQALHHTGGLPDYIRDERFIARLQADPTAYMSPRRLVRFVRDE
                     PLRFRPGSRYEYSDTDNIVVALMAEAATGVSYERLLARHVYRPVGLRATSLPRSVRMP
                     RPFMRGYEVAPGLPAEDVTRAINPALAWASGGIVSTASDVNYFFRAYAGGDLFGARER
                     RAQRRFRPGSSSPPGPGRNAAGLGIFRYSTGCGTVYGHTGSFPGYRLFAASSADGRRS
                     VAFFVNAQIVPGQGSAAVSNQIRAAQAAAVCHALR"
     gene            complement(6248..6757)
                     /locus_tag="VHF88_01885"
     CDS             complement(6248..6757)
                     /locus_tag="VHF88_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160551.1"
                     /translation="MGVLDDAIREHLELKRKHGASEGEVERQEQEALGAPRRQPAAPS
                     EEAGAAAGEEQAPAPVEETAPPEAEVADPAPESVEAPPEPEPEPEPEPEPEPEPEPEP
                     AAAEPDPPAAAGDTPPRGFESVEDEAPPTDGGEEDVLEETPDFLQETPEHDRLWFEQK
                     PPRDFDFDE"
     gene            6823..6895
                     /locus_tag="VHF88_01890"
     tRNA            6823..6895
                     /locus_tag="VHF88_01890"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6856..6858,aa:Ala,seq:cgc)
     gene            complement(6937..7377)
                     /locus_tag="VHF88_01895"
     CDS             complement(6937..7377)
                     /locus_tag="VHF88_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2160552.1"
                     /translation="MTIKRMDNVLIVVDDLEAAKAFFVELGMELEGETQVEGDWAGSA
                     VGLDDVRADIAMMRTPDGHGRVELSRFHTPPAVRAEPESAPANALGIRRIMFAVDDID
                     DVVARLRDHGGELVGEIAQYEDSYRLCFLRGPEGIVIGLAEQLG"
     gene            7536..7736
                     /locus_tag="VHF88_01900"
     CDS             7536..7736
                     /locus_tag="VHF88_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160553.1"
                     /translation="MRWERGHKETAAAGLVSLALAGLLFRPSSATAAVVAVLAIVAFV
                     AAIDVLAKREPQRPDRLLKRLR"
     gene            complement(7733..8071)
                     /locus_tag="VHF88_01905"
     CDS             complement(7733..8071)
                     /locus_tag="VHF88_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX2160554.1"
                     /translation="MSIVVYEKRTCTTCRNLAELLTERGIDFDRVQYHVEGLDERELR
                     DLIAKTGVPVADLLRMREDGARELAENGDEDAIVAAMVDRPELLQRPIVVNGDRAVLA
                     RPVERVLEIL"
     gene            complement(8103..8747)
                     /locus_tag="VHF88_01910"
     CDS             complement(8103..8747)
                     /locus_tag="VHF88_01910"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2160555.1"
                     /translation="MTLASLPIALGPSWLDPETLIEAFGMLGVLAIVFIESGLLVGFF
                     LPGDSLLFTAGLLSANDVLPDIWVLLVTIPIAAIAGDQVGYAIGRKAGPAIFRRPDSR
                     FFHHENVERAQGFFDRYGPRTVVLARFVPIVRTFVPVVAGVAQMKYRTFLTYNIVGGI
                     LWGVGVTTLGYHLGQIDFIKANIEFVLIGIVGLSLIPVAVELYRGDRRRRATAS"
     gene            complement(8764..9249)
                     /locus_tag="VHF88_01915"
     CDS             complement(8764..9249)
                     /locus_tag="VHF88_01915"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2160556.1"
                     /translation="MPHPVRPVDDSFFETAPLVVPVTVEFASSPTEVWEALGSDAMWS
                     WFPPLDRLRWLTSRPHVEGAVRVVRVARLFTIEEHLYRWEEGRRATFHVVSSTRPVLD
                     ALAEDFVLEPTASGTRLTWTLALDPKLPGARRLGPLLAPLLKPGNRRAIAGLRTILPR
                     G"
     gene            9312..10328
                     /gene="gnd"
                     /locus_tag="VHF88_01920"
     CDS             9312..10328
                     /gene="gnd"
                     /locus_tag="VHF88_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX2160557.1"
                     /translation="MQLGMIGLGRMGANMVLRLMRDGHDCVAFDLNTDAVAKVADEGA
                     TGAESFADLVAKLDKPRAVWMMVPAGVVDQTIAELAPHLEEGDTLIDGGNSYYHDDLR
                     RSAELRERGIHYLDVGVSGGVWGLERGYCHMIGGDEEAVVRLEPIFESLAPGVDAAPR
                     TPGREGEPSQAEKGYLHCGPSGAGHFVKMVHNGIEYGVMAAYAEGLNVLRHANVGKRE
                     HEVDAETTPLRDPALYQYDLDLPEVAEMWRRGSVIGSWLLDLTASALRKSPDLEDFGG
                     RVSDSGEGRWTAIAAIDEAVPTPVLTSALYERFSSRGESDFADKLLSAMRFEFGGHHE
                     KPGS"
     gene            10359..11087
                     /locus_tag="VHF88_01925"
     CDS             10359..11087
                     /locus_tag="VHF88_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX2160558.1"
                     /translation="MLGLPDTVRACLFDMDGVLTDTASVHAEAWKRMFDEYLRAKVGP
                     DAAPFDIASDYGLYVDGKPREDGVRDFLASRGIVLPEGTPDDGPEDDTVYGVGKRKNV
                     LVLKLIDERGVDVYAGSVRFVDAARAAGLKTAVVSSSANTKLILESAGLDDRFDARVD
                     GVTLATDNIKGKPAPDSFLRAAELLGVPATAAAVFEDALAGVEAGRAGGFGITVGVDR
                     HGQPDALREHGADVVVSDLAELLT"
     gene            11084..13486
                     /locus_tag="VHF88_01930"
     CDS             11084..13486
                     /locus_tag="VHF88_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX2160559.1"
                     /translation="MRDQDRHPPEPWAFVERGIKLDRLAQTESLFALANGHIGLRGNL
                     DEGEPVGVSGTYLNGFYESYPLQYGERGYGFAEDGQTVVNVPDGKIVRLQVEDEPLDI
                     HRGTLEHHERRLDFRSGVLERDLRWRTASGVAVKVSTKRLVSLSLRSVAAILYEVEAV
                     DQKARIALQSNLQVNRMRAQELDDPRGGRHIEDALRSELSVHDDVRVVLGHVTRASGL
                     SMASGMDHVIECDSDVEQLTQSEADLGRVTMSVELQPGEKLRVVKFVSYHWSSQQSVE
                     WLRDQVDASLESAIAEGFDGLVEEQREILDRYWERADIQVEGDEELQQALRFAMFHIF
                     QSAARNEGRAIPAKGLTGTGYDGHAFWDTETFVLPLLTFTAPATVRHALIWRHSILDA
                     ARDRAEQLHLRGAAMPWRTIHGEECSGYWPAGTAAFHVNADIADAVRRYVMVTGDVDF
                     EEKYGCELLVETARLWESLGHHDDYGTFRIDGVTGPDEYSALVDNNVFTNLMAQLNLE
                     SAYACTDRHPEVAKRLGVDEEEREAWKRAADAMYIPFDEKAGVHPQDDSFLHHQRWDF
                     ENTPEENYPLLLHYPYFQLYREQVIKQADLVLAMQIRGDAFTPEQKLANFDYYEPLCV
                     RDSSLSAATQAVIAAEVGHLELAKDYLYEASHMDIADLERNTADGLHMASLAGAVIAT
                     VAGFGGLREQGGKLRFQPRLPEGIDRLCFGFELRGTLLRVEVLPGEATYRVERGDGIH
                     LTHWGEAVDLTAKHAATLPIPAAPDLPTPSQPPGREPGRRRPRRPIDYAPLEARPLED
                     RA"
     gene            13489..13989
                     /locus_tag="VHF88_01935"
     CDS             13489..13989
                     /locus_tag="VHF88_01935"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX2160560.1"
                     /translation="MTGRGGDDGVVHDLVASLDYPMFIVTVAAGDERAGCLVGFATQC
                     SIDPPRFLVCLSERNRTFEVAARTELLIVHMVPSEATELADLFGGETGDEVDKFARCD
                     WRPGPGGAPVLDGCENWFAGRVLERFAVGDHHAYLLEPCAAHSGDDHGQFNFHRAKRI
                     EAGHPA"
     gene            14037..14207
                     /locus_tag="VHF88_01940"
     CDS             14037..14207
                     /locus_tag="VHF88_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160561.1"
                     /translation="MTEQKDDMKPGDEVPPGTDAAGPAPCPQCGGDGKVDGGECDLCR
                     GTGEIEQPVGGG"
     gene            14642..15781
                     /locus_tag="VHF88_01945"
     CDS             14642..15781
                     /locus_tag="VHF88_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2160562.1"
                     /translation="MDAWVASAAEQIAARAERDLEALVAVSSPSGDVEGAEECIALVG
                     ALAPPEAEIERRPCSSPGHADDVLLRLGGSGSARLLLLGHIDTVVGHASHRPVERDGG
                     RLVGSGSIDMKGGDVLALGVLRALAERTADFAEVALLLVTDEEWRSAPFAHVEAFAGW
                     DACLCFEAGQRTAEGEEAVIVRRKAAATLRVSAAGRSAHSGSAPERGRNALLALAAAA
                     QAVAAANDPAGADELTAVPTILRSGDAFNVVPGDGELFCDLRSNRTDAFDEVRALVPD
                     EVGGATLGTQFIRLWPAMDARAATAPLIAAAADRLRRPIAAAGRGGASDASHFASVVP
                     VTIDGLGPRGGGAHASDEYVLAESLYPRAEVALAIVDAVLTSGAR"
     gene            complement(15778..17331)
                     /locus_tag="VHF88_01950"
     CDS             complement(15778..17331)
                     /locus_tag="VHF88_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2160563.1"
                     /translation="MSVGMARRAGQDDEGGLSRRELLGAAGVAGIAGLGVAGGSGGWR
                     SISRALAASERRNVVLIVLDSLRADHLRCYGARGMRTPNIDELAGGSMRFTHVFPEAM
                     PTMPARRSIMSGRHAYPFRGWRPWEGMAGRPGWQPIAPGSETLITALRRAGWWTSYVT
                     DNPFLGYTRALEPFRRTPDRFVRVHGQRGARRPRKSVPRSAALRRLPPGPLRNERRIA
                     SIQQYLANNGRGHNEVEQAASRVFRSAAKLLPIAKRQKQFLMVVDSFDPHEPWIPPRH
                     YLDMYGDPDYRGYEIAEVGYTYASNYLEAAQIARLQTTYKAAVTMTDRWLGLFLDRLW
                     KLGLDDSTAIVLVSDHGVFLGERNWTGKGPGKLYPELIHVPLLLREPGGAGAGQASNW
                     FASTHDVAPTLASLAGIGRPSTFEGADLTPVLTAGLPDEDRPYAVGGYGNSTYVRDYR
                     WAYQVQNDGRNERLYDLRADPGEHVDVSERRPRVVAEMRRRVLAAAGGKPPYYSDAVI
                     EGPRRRYRR"
     gene            17499..18962
                     /locus_tag="VHF88_01955"
     CDS             17499..18962
                     /locus_tag="VHF88_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2160564.1"
                     /translation="MESQARPNVVVLMTDDQTLRSMRAMPRTRALIGAQGARFANSFV
                     SNPRCCPARATFLTGQYSHNNGVLTNGPPLGGWQRLRGTSNWLPTWLRSAGYRTVHVG
                     KFLNHYGVDDPHEVPPGWDEWYGTIDPTTYRYYGYTVNENGELRTYGRDHDPRWYSTD
                     FASRRSAGIVERLAPETQPFFLSVAFLAPHTGGPYEAGDPTSLGTPAVAERHRGAYAS
                     LPLPRLAAFDEADVSDKPPFLRRPRLRPRAIADIEALYRQRLESLLAVDEGIEQIVDA
                     LRRSGELHNTLIVFTSDNGYMAGEHRLPHGKAKAYDPSIRVPLMIRGPGVPVGVEPQQ
                     MVVNADLAPTILEAARATAGRVQDGRSLLPLTRDPSLGTGRPVLVEIAKGAGMKTTAI
                     RTPRWLYAEHAGGWSELYDVVADPQQLRSLHADPTLAGVRARLASQLAVLRACAGLSC
                     RQPFAEPARRARRRHGAGTRRLGKRPVHRNSRSPRRF"
BASE COUNT         2735 a         6915 c         6802 g         2619 t
ORIGIN      
        1 gatcgcggcg agcggcgcgg ccggggcaag cggcgcggtc gccggcgatc gagctggcgg
       61 caccggcgcc gcgcccgtcg gctcgtcctt cgagccgagc tcgccggcgc cgatcgagct
      121 gctcggcgtc gatgcgttcg acgtgaagtg gcgcatgccg ccgcgggcgg cgctcgtctt
      181 cgacgtcgag aacggcgact cgctctaccg gcgccgcacg acacacgagc tgccgatcgc
      241 cagcctgacg aagatcatga ccgcgctcgc ggtgacggcg gcgaccgagc ccggcgaggt
      301 ggtgcgggtc ccgaaggcgc tgcgctacga gggctccgct gtcgggctgc tgccgctcgg
      361 gcgtcgcgtg cggatcgagc cgctgctcgc cggcctgatg atcgtctcgg gcaacgacgc
      421 ggcactggcg ctcgccgacc acgtctcggg cagcgagcga cgcttcgtca ccgcgatgaa
      481 cgagcaggcg cggctgtggg acctcgactg cacccacttc gcgacaccgc acggactctc
      541 cgacgccgac cgctcctgcg ccgtcgacct ggcggtcatg acgcggctcg cgatgcgcgt
      601 accgcggatc gtcgacctca gccgccgcgc ccaggccgcc taccgcttcc cgatcaaggg
      661 ccgcaagctc tacctctccg gccacaaccc gctgatccgc gccggctacc ccggcgcgat
      721 cggcctcaag accggctata ccgacgccgc cggccgctgc ttcgtcggcg tcgcccggcg
      781 ccacggccga accttcggcg tcgtcctgct cgactcgccg gaccccggcc gccaggccgc
      841 ccgcctgctc gacctcgcgt tcgagttcgg ctgagcggcg ccggccgtcc ccgctgaggg
      901 attcgcacgg cctggactgc agaacccctc agtcggccat cgcgagctac ttcttgcggt
      961 ctgtgtcacg cgacggctgg acgcgcttcg gctcccccgg catcttcggg tagtcgggcg
     1021 gatacggcat gtcgccggcg ccctcggcct cgtcgcgctc gtacatctcg agcagcggct
     1081 cgagcgagtg ggcgacgtcg tcgatcgtcg cgaagcggtc gccgacctcg gcgaagcggg
     1141 ccggcatcgt ggcgaccgtg aagtcctcag gcgccacctc ggtcagctca tcccagctca
     1201 gcggcgccga caccggcgcc cccggcttcg ggcggacgct gtagggcgag gcgatcgtgc
     1261 ggtcgcgggc gttctggttg tagtcgacga agatcttctc gccgcgctcc tccttccacc
     1321 acttggtcgt cacctcgccg ggcagccgcc gctcgagctc gcgcccgaag gcgatcgcgg
     1381 cgtggcggac gtcggtgaac gtccagcgcg gctcgatccg cacgtagatg tggatgccgc
     1441 ggccgccgga ggtcttcgga aagccggcgt agccgagctc gccgagcagc tcgcgcgcca
     1501 ccccggcgac gcgcaccgcg tcgtcgaagt cggtccacgg ctgggggtcg aggtcgagcc
     1561 gcagctcgtc gggatggtcg acgtcggcgc ggcgcaccgg ccacggatgg aaggtgagcg
     1621 tgcccatctg ggcgcaccag ccgacggtcg cgatctcggt cgggcagacc tcgtcggcgc
     1681 tgcggccgga ggggaactcg atccgcgccg tctcgacgta gtcaggcgcg ccctttggga
     1741 cccgcttctg gtagaaggcg tcgcccttct gaccctccct tgtggtgagg gtcatcccct
     1801 cgtggacgcc cttcggccag cgctccagcg tcgtcggccg ctcgcgcagc gcgcgcatga
     1861 tgccgtcctc gaccgccagg tagtactcga cgatctgcag cttcgtgatc tcggccgtcc
     1921 gctcggtcgc cgggaagatc acccggtccg cgctcgtcac gcgcagctcc cgcccgcccg
     1981 cctcgagcat cgtcgcctcg gccttggcca tcgcctcagg atgccgcacc cgccggccgc
     2041 cttgcttggc gctcagggcc ggtcgtgcga acattcccag cgaggggcca tagctcagct
     2101 ggaagagcgc ctgctttgca agcaggaggt cgccggttcg agcccggctg gctccatcgc
     2161 ggacagagtc gtggcccggc gttcgacatc ccggcgcgag cacttgcaat gcgggacgcg
     2221 cagagctaca ttgccgcggc tcagaccacc gttccagaag gggattcgat gaagcagagc
     2281 ctctctagcg ccggccggct ggcccgatac ggcggcgcta ccgctggcgt cctggccgcc
     2341 gcgttgatcg gcggcccgac cgtgctcgcc caggatggcg atacgccggc cgagccgacc
     2401 acgatcacga tggaggcgca gggcaagcgc ctcgtcttcg acggcccgga gaccgtccag
     2461 aagggcgacg agctgcggat cgtcaacgac acgagcgtgc gcaagtacgg gccgcacacg
     2521 ttctcgctcg tcaagcagag cctgctgccg gactcgggca aggagttcag gcagtgcttc
     2581 acgccaggca agatctgcat ggagatcgcg atggcgcaca agttcaatcc ccgcaccgag
     2641 aaggtcggca agccggtcgt gaacgccgga aagaggggct gggaccgcag cttcaacggc
     2701 aagcgcaacg gcgactcgtg gtacacggac aagaaggacc agtccttcac gcgccgggtc
     2761 acggcgaagg ccgggacgac gctgagcttc atctgcgcgg tccatccgga catgcagggc
     2821 gagatcgacg tcgtcgacgc ggagtaagcg acgctcgagg ggctccgccg cgacggcgga
     2881 gccccttcgc aagccgccgg tgaaagcgat ccaggacacg gctgagcgcg ggctgtcgcg
     2941 gcgggccctg ctggccggtg cggcggccgg ggcgatcacg ctcgggctgc cgggccgggg
     3001 acgcacgagc gtgcatccgc ccgtccggct gcggccgttc gcgcagccgc tgcgcatccc
     3061 ggaggtgctg actgggcccg agctggcgat cccgatgcgc cacgcggagg tcgacgtcct
     3121 gccgggccgg cccacccgga tgtggaccta cgacggcagc ttccccggtc cgacggtccg
     3181 gcggcccgcc ggcgagacca cgcgcgtgcg cttcgaccac cggctcggag agcgggccgg
     3241 cgagctgacc gtccacctcc acggcggcca caaccgcgcg agcgaggacg gccagccggg
     3301 cgggctgacc gcgcgccagc cgcgctcgct ctactgcgac atctcgcccg gcctgacggc
     3361 ggccgagtcg ggcaacgacc tgctgatcgc gccgggcggc tcgcgcacct acagctacga
     3421 gcaggtcgag gacggacagc ccgagcgggc gtcgttccag tggtaccacg accaccgcct
     3481 cgatcgcacg gcgcgcaacg tctggcgcgg gctagcgggg atgtggatcg tcgacgacga
     3541 gctcgacgcg tcgctgccgc tgccgcgcgg ccggcgcgac atcccgctga tgctcgccga
     3601 ccggtccttc gatcggcgca accagctgac cgacccgttc gccggcggcg gactgcgccc
     3661 gccggacgac ggcgtcgtcg gccggcacat cctcgtcaac ggcgtcgtgc tgccgcatca
     3721 cgacgtacgg ccgcagcgct accggctgcg cgtcctcaac gcctctcagt tccgctcgta
     3781 caacgtctgc ctgacccgcg gcgtgaagat cgtccagatc gcgaccgagg ggggcctgct
     3841 gccacggccg ttgcgtcgcc gtgcggtgct gctcgggccg ggcgagcgcg ccgagctgat
     3901 cgtcgacttc gcgcaggcgc gcggcgagcg cgtccagctg gtcagtgtcc cgcgacgcgg
     3961 cgcgccgcac aagcttggat cgcagtcata cgcggggccg ctgatggagt tccgcgtcgg
     4021 cagccggcgg ctgcgcgaca ccacggcgat ccccgccgcg ctgcgaccgc tgccggcctg
     4081 gatcgccgat gcgccatcca agccgtcgca cagctggaag ctgaccgtcg gcctcggtct
     4141 acggccgagc tggctgatca acggccgcac ctacgacccg ggctacgtcg agacctcggc
     4201 cgcgctcggc gaggtcgtca cctgggagct gcacaaccgg accgcggccg cccacctgat
     4261 gcacctccac cacaccgact ggtacatgct ctcgcgcaac ggccgccgcc cgccgccgtg
     4321 ggagcgctgc ctcaaggaca ccttcttcct cgaccccggc gagcgggtcg tgatcgccgg
     4381 caagctcagc gactatcccg gcaggtacgt cgtccactgc cacatgctcg accacgagga
     4441 ccacggcctg atgtcgcagt tcgaggtgac cgcggcgtag gtccaggtct cagttgagga
     4501 tcgccctgct cagccggcgg ccctcgctgt agggcggcat gtggatcgag acgacgactt
     4561 cgccgcgctg ggcgaccagg acccactcgc ggtccgagag ccaaccggcg aaggcgtcgc
     4621 cgcggacgag gtcggtcgac gacagccgtg ctccgctgtc gtgctcgatc tggccgcgag
     4681 tgaacgagac atgagctcca ccgtcgcgcc cgatcaccgt cacggtcgcg gcgcgcatgc
     4741 agccgcgcag caccagcagc gtcgccgtgt gccagtcgtc ggtggcggga tcggtgtccg
     4801 gctggagctc gtagcgataa ccgggcggca gctcgccgag cagctcccgc tcggggctcg
     4861 cgacggcggg cggcacgtcc ggcgccgcgt tccagaccat gcccaccacg agcgcggtcc
     4921 acagcagcag cgcgaccggc cagaggcgaa gacgtcccga tcgatcccgg aatggcccca
     4981 acatctctcc cgccttggag taagactatg ttcctattcc cagccggacc gatgagtact
     5041 catcgagacc cagggggcgc tgcggtaggc ggctcagcgc agcgcgtggc agaccgcggc
     5101 ggcctgagcg gcgcggatct ggttcgagac ggctgccgag ccctggcccg gaacgatctg
     5161 ggcgttgacg aagaaggcga ccgagcggcg gccgtcggcg ctgctggccg cgaacaggcg
     5221 gtagccgggg aacgagccag tgtggccgta gaccgtgccg cagccggtgc tgtagcggaa
     5281 gatgccgagt ccggcggcgt tgcgccccgg accgggcggt gacgaggacc ccgggcggaa
     5341 gcgccgctgg gcgcgccgct cgcgggcgcc gaagaggtcg ccgccggcgt aggcgcggaa
     5401 gaagtagttg acgtcggagg cggtcgacac gatcccgccc gacgcccagg cgagcgccgg
     5461 attgatcgcg cgcgtgacgt cctcagccgg caggccgggc gccacctcgt agccgcgcat
     5521 gaacggccgc ggcatccgga cgctgcgcgg caggctggtc gcgcgcaacc cgaccgggcg
     5581 gtagacatgg cgcgcaagca ggcgctcgta ggagacgccg gtcgccgcct cggccatcaa
     5641 cgcgacgacg atgttgtcgg tgtcggagta ctcgtaacgg ctccccggcc ggaagcgcag
     5701 cggctcgtcg cgcacgaacc tgacgagccg gcgcggcgac atgtaggcgg tcgggtcggc
     5761 ctgcaggcgg gcgatgaacc gctcgtcgcg gatgtagtcg ggcaggccgc cggtgtggtg
     5821 cagggcctgg cgcagcgtca cccgccgcgc ccgcggcagc agaccgggca acagctcgcc
     5881 gacggtatcg cgcagctcga gctcgccgcg gtcgaccagc gcgagcgcca cagcgccgga
     5941 gaaggccttc gagacgctcg cgatgcggac gtggtcgtgg acgccggggg gcgtgtctcg
     6001 cgcgaggtcg ccgacccctc gccggcggta ctcgcgccgc tcgccgcgct ggatcaggac
     6061 cgagacgccg ggcgggccgg cgggcgtcgc cgcgagccgg tcgagcgtcg cgtcgagcac
     6121 ctgggcggtg cctggcggat cagcggccgc ggcctgagct ggaacggcga tcgctgcagc
     6181 catggccacc aacacggccg acgaccgtcg cgagcaagcg agcatcgtcg ccgaggccac
     6241 ccttcgccta ctcgtcgaag tcgaagtcgc gcggcggctt ctgctcgaac cagagccggt
     6301 cgtgctcggg ggtctcctgg aggaagtccg gcgtctcctc gagcacgtcc tcctcgcccc
     6361 cgtccgtagg cggcgcctcg tcctcgacgc tctcgaagcc gcgcggcggc gtgtcgcccg
     6421 cagccgcggg cgggtccggc tcggcggccg ccggctcggg ctcgggctcg ggctcgggct
     6481 cgggctcggg ctcgggctcg ggctcgggct cgggcggcgc ctctaccgac tcgggtgccg
     6541 ggtcggcaac ctcggcctcg ggcggcgccg tctcctcgac cggcgcgggc gcctgctcct
     6601 cgcccgccgc cgctccagcc tcctccgacg gcgccgccgg ctgccgtcgc ggcgcgccga
     6661 gcgcctcctg ctcctggcgc tccacctccc cctccgaagc gccgtgcttg cgcttcagct
     6721 cgaggtgctc acggatcgcg tcgtcgagaa cgcccatcgc tgcgacagcc tacgccacgc
     6781 tcccgatgcc cggtagcatt gaccctcgcc cagtgggcgc ctggggctgt agctcagctg
     6841 gaagagcgct gccttcgcat ggcagaggtc aggggttcga gtcccctcag ctccatcgga
     6901 gggagcccag aagcgccgct tccgcaccca ggggcttcag ccgagctgct cggccagccc
     6961 gatgacgatg ccctctgggc cgcgcagaaa gcagagccgg tagctgtcct cgtactgcgc
     7021 gatctcgccg acgagctcgc caccgtggtc gcgcaggcgc gcaacgacgt cgtcgatgtc
     7081 gtcgacggcg aacatgatgc ggcggatgcc gagcgcgttc gccggcgcgc tctcgggctc
     7141 ggctctgacc gccggcggcg tgtggaacct cgacagctcg acccggccgt gaccgtccgg
     7201 ggtccgcatc atcgcgatgt cggcacggac gtcgtcgagc ccgacggcac tccccgccca
     7261 gtcaccctcg acctgcgtct cgccctccag ctccatgccg agctcgacga agaacgcctt
     7321 ggcagcctcg aggtcgtcga cgacgatgag gacgttgtcc atccgcttga tcgtcatatc
     7381 ggcggagcat agggcgggaa gccggccgcc tcggcgatca attcgacttc ctcaactccc
     7441 ttcgccgtta cacgagcgcc ttgtccggac gcaagctcag ggacgtgtcg tcacccctcc
     7501 tcgaaaccgt cggttcgagc gtatagacta tctgcatgcg ctgggagcgg ggccacaaag
     7561 agacggcagc tgccggactg gtgtcgctcg cgctagccgg gctgctgttc cggccatcct
     7621 ccgcgacggc cgccgtcgtc gccgtgctgg cgatcgtggc tttcgtcgct gccatcgatg
     7681 tgcttgcgaa gcgtgagccg cagcgccctg accggcttct gaagcgcttg cgctagagga
     7741 tctcgagcac gcgctcgact gggcgcgcga ggacggcgcg gtcgccgttg acgacgatcg
     7801 ggcgctggag cagctcgggg cggtcgacca tcgcggcgac gatggcgtcc tcgtcgccgt
     7861 tctcggcgag ctcgcgtgcg ccgtcctcgc gcatgcggag caggtcggcc acggggacgc
     7921 ccgtcttggc gatcaggtcg cgcaactcgc gctcgtcgag gccctcgacg tggtactgga
     7981 cgcggtcgaa gtcgatgccc cgctcggtga gcagctccgc caggttgcgg caggtggtgc
     8041 aggtgcgctt ctcgtagacg acgatcgaca tcgtctgggc aatctagtac gcggccggac
     8101 ggtcaggaag ccgtcgcacg ccggcggcgg tcgccgcggt agagctcgac ggcgaccggg
     8161 atcagcgaca gcccgacgat gccgatcagg acgaactcga tgttcgcctt gatgaagtcg
     8221 atctggccaa ggtggtagcc gagcgtggtg acgccgacgc cccagaggat gccgccgacg
     8281 atgttgtagg tgaggaaggt gcggtacttc atctgcgcga cgccggcgac gacggggacg
     8341 aacgtgcgga cgatcgggac gaagcgggcg agcacgacgg tccgcgggcc gtagcggtcg
     8401 aagaagccct gtgcgcgctc gacgttctcg tggtggaaga agcggctgtc gggccgacgg
     8461 aagatcgcgg ggccggcctt gcggccgatc gcgtagccga cctggtcgcc ggcgatcgcc
     8521 gcgatcggga tcgtcaccag cagcacccag atgtcgggca gcacgtcgtt ggccgacagc
     8581 agcccggccg tgaacagcag cgagtcaccc ggcaggaaga agccgaccag cagccccgac
     8641 tcgatgaaga cgatcgccag cacgccgagc atcccgaagg cctcgatcag ggtctcggga
     8701 tcgagccagc tcgggccgag cgcgatcgga agcgaggcga gcgtcacgcg ggcaccgtag
     8761 cggtcagccg cgcggaagga tcgtgcgcag cccggcgatg gcccgccggt tgcccggctt
     8821 cagcagcggc gcgagcagcg gtccgaggcg ccgagcgccc ggcagcttcg ggtcgagcgc
     8881 cagcgtccag gtcaggcgtg tgccgctagc cgtcggctcg agcacgaagt cctccgcgag
     8941 cgcgtcgagc accggccggg tcgagctgac gacgtggaag gtcgcccgcc gtccctcctc
     9001 ccagcggtag aggtgctcct cgatcgtgaa cagccgcgcg acgcgcacca cgcgcaccgc
     9061 tccctcgaca tgcggccgcg acgtcagcca gcgcagccgg tcgagcggcg ggaaccacga
     9121 ccacatcgca tcgctgccga gcgcctccca cacctcggtg ggcgacgacg cgaactcgac
     9181 cgtcaccgga accaccagcg gcgcggtctc gaagaagctg tcgtcgacgg ggcggacggg
     9241 gtgcggcacg ggcggcagga tggcaggcgc ctgcctagag tgggctcacc tcgaccgaac
     9301 aggagtcacg gatgcagctc gggatgatcg gcctcggacg gatgggcgcc aacatggtgc
     9361 tgcggctcat gcgcgacggt cacgactgcg ttgcgttcga cctgaacacc gacgcggtgg
     9421 caaaggtggc ggacgagggc gcgacgggag cggagagctt cgcggacctc gtcgccaagc
     9481 tcgacaagcc gcgcgcggtc tggatgatgg tgccggccgg cgtcgtcgac cagacgatcg
     9541 ccgagctggc accgcacctc gaggagggcg acacgctgat cgacggcggc aactcctact
     9601 accacgacga cctgcgccgc tcggcagagc tgcgcgagcg cgggatccac tatctggacg
     9661 tcggcgtcag cggcggcgtc tgggggctcg agcgcggcta ctgccacatg atcggcggcg
     9721 acgaggaggc cgtcgtgcgg ctcgagccga tcttcgagtc gctggcgccg ggcgtcgacg
     9781 cggcgccgcg caccccgggc cgcgagggcg agccgtcgca ggccgagaag ggctacctgc
     9841 actgtggccc gtctggcgcc ggccacttcg tgaagatggt ccacaacggc atcgagtacg
     9901 gcgttatggc cgcctacgcc gaggggctga acgtcctccg ccatgccaac gtcggcaagc
     9961 gcgagcacga ggtcgacgcc gagaccacgc cgctgcgcga cccggcgctc taccagtacg
    10021 acctcgacct gcccgaggtc gccgagatgt ggcggcgcgg cagcgtgatc ggatcctggc
    10081 tgctcgacct gaccgccagc gcgctgcgca agagccccga cctagaggac ttcggcggcc
    10141 gcgtgtcgga ctccggcgag ggccgctgga ccgcgatcgc ggcgatcgac gaggccgtcc
    10201 cgacgcccgt cctgacttcc gccctatacg agcgcttctc ctcgcgcggc gagtccgact
    10261 tcgccgacaa gctgctctcc gccatgcgct tcgagttcgg cggccaccac gagaagcccg
    10321 gtagttagac ccgccggggg ttgtgcgacg cttgcgcgat gctcgggctg cccgacacgg
    10381 tgcgcgcctg cctgttcgac atggacggtg tgctgaccga caccgccagc gtgcacgcgg
    10441 aggcgtggaa gcggatgttc gacgagtacc tgcgcgcgaa ggtcgggccg gacgccgcgc
    10501 cgttcgacat cgcgtcggac tacgggctct acgtcgacgg caagccgcgc gaggacgggg
    10561 tgcgcgactt cctggcgtcg cgcgggatag tgctgcctga ggggacgcca gacgacggcc
    10621 ccgaggacga cacggtctac ggagtgggta agcgcaagaa cgtgctggtg ctgaagctga
    10681 tcgacgagcg gggcgtcgac gtctacgcgg gctcggtgag gttcgtcgac gccgcgcgcg
    10741 ccgcgggcct gaagaccgcc gtggtctcgt cgagcgcgaa cacgaagctg atcctcgaga
    10801 gcgccgggct cgacgaccgc ttcgacgcgc gcgtcgacgg cgtgacgctc gcgaccgata
    10861 acatcaaggg aaagccggcg ccggactcct tcctgcgcgc cgccgagctg ctcggcgtgc
    10921 cggcgaccgc ggcggcggtc ttcgaggacg cgctcgccgg cgtcgaggcc ggccgcgccg
    10981 gcggcttcgg catcaccgtc ggcgtcgatc gccacggcca gccggacgcc ctgcgtgagc
    11041 acggcgcgga cgtcgtcgta tccgacctag cggagctgct gacatgagag accaggaccg
    11101 ccacccgccc gaaccgtggg cgttcgtcga gcgcggcatc aagctcgacc gcctcgccca
    11161 gacggagtcg ctcttcgcgc tcgccaacgg ccacatcggg ctgcgcggca acctcgacga
    11221 gggcgagccg gtcggggtca gcggcaccta cctcaacggc ttctacgagt cctacccgct
    11281 ccagtacggc gagcgcggct acggcttcgc ggaggacggc cagacggtcg tcaacgtgcc
    11341 cgacggcaag atcgtgcggc tgcaggtcga ggacgagccg ctcgacatcc accgcggcac
    11401 gctcgagcac cacgaacggc ggctcgactt ccgctctggc gtgctcgagc gcgacctgcg
    11461 ctggcggacc gcctccggcg ttgcggtgaa ggtgagcacc aagcgactgg tctcgctgtc
    11521 gctgcgcagc gtcgcagcaa tcctctatga ggtcgaggcc gtcgaccaga aggcgcggat
    11581 cgcgctgcag tccaacctcc aggtcaaccg catgcgcgcg caggagctcg acgacccgcg
    11641 cggcggccgc cacatcgagg acgcgctgcg ctcggagctg tcggttcacg atgacgtgcg
    11701 cgtcgtgctc ggccacgtca cgcgcgcgtc gggactgtcg atggcctcgg gcatggacca
    11761 cgtgatcgag tgcgacagcg acgtcgagca gctgacccag tccgaggccg acctcggccg
    11821 tgtgacgatg tcggtcgagc tgcagccggg cgagaagctg cgcgtcgtca agttcgtctc
    11881 ctaccactgg tcctcgcagc agagcgtcga gtggctgcga gaccaggtcg acgccagcct
    11941 cgagagcgcg atcgccgagg gcttcgacgg cctcgtcgag gagcagcgcg aaatcctcga
    12001 ccgctactgg gagcgcgccg acatccaggt ggagggcgac gaggagctgc agcaggcgct
    12061 gcgcttcgcg atgttccaca tcttccagtc ggcggcacgc aacgaggggc gcgcgatccc
    12121 ggccaagggg ctgaccggca ccggctacga cggccacgcg ttctgggaca ccgagacctt
    12181 cgtgctgccg ctgctcacct tcaccgcgcc ggcgaccgtg cgccacgcgc tgatctggcg
    12241 ccattcgatc ctcgacgccg cgcgcgaccg cgccgagcag ctgcacctgc gcggcgccgc
    12301 gatgccgtgg cggacgatcc acggcgagga gtgctcgggc tactggccgg cgggcaccgc
    12361 cgcgttccac gtcaacgcgg acatcgccga cgccgtgcgg cgctacgtga tggtcaccgg
    12421 cgacgtcgac ttcgaggaga agtacggctg cgagctgctg gtcgagacgg cccggctctg
    12481 ggagagcctc ggccaccacg acgactacgg caccttccgg atcgacggcg tcaccgggcc
    12541 ggacgagtac tcggcgctcg tcgacaacaa cgtcttcacg aacctgatgg cccagctcaa
    12601 cctcgagtcg gcctacgcct gcaccgaccg ccaccccgag gtggcgaagc ggctcggcgt
    12661 cgacgaggag gagcgcgagg cgtggaagcg cgctgccgac gcgatgtaca tcccgttcga
    12721 cgagaaggcg ggcgtccacc cccaggacga cagcttcctc caccaccagc gctgggactt
    12781 cgagaacacg cccgaggaga actacccgct gctgctgcac tacccgtact tccagctcta
    12841 ccgcgagcag gtgatcaagc aggccgacct cgtgctggcg atgcagatcc gcggcgacgc
    12901 cttcacgccg gagcagaagc tcgccaactt cgactactac gagccgctct gcgtgcgcga
    12961 ctcgtcgctg tcggcggcaa cgcaggcggt gatcgccgcc gaggtcggcc atctcgagct
    13021 ggccaaggac tacctctacg aggcgtcgca catggacatc gccgacctcg agcgcaacac
    13081 cgccgacggg ctgcacatgg cgtcgctggc cggcgcggtg atcgccacgg tcgcggggtt
    13141 cggcgggctg cgcgagcagg gcggcaagct gcggttccaa ccgcggctgc ccgaggggat
    13201 cgaccggctc tgcttcggct tcgagctgcg cggcacgctg ctgcgggtcg aggtcctgcc
    13261 tggggaggcg acctaccgcg tcgagcgcgg cgacggcatt cacctgacgc actggggcga
    13321 ggcggtcgac ctgaccgcca agcacgccgc gacgctgcca atcccggcgg cgcccgacct
    13381 tccgacgccc tcgcagccgc cgggccgcga gccggggcgg cgccgaccgc gacgaccgat
    13441 cgactacgcg ccgctcgagg cgcgcccgct cgaggaccgc gcctagcggt gaccggccgc
    13501 ggcggcgacg acggtgtggt ccacgacctc gtcgcgtcgc tcgactaccc gatgttcatc
    13561 gtcaccgtcg cagcgggcga cgagcgggcc ggctgcctgg tcggcttcgc gacgcagtgc
    13621 agcatcgatc cgccgcgctt cctcgtctgc ctctccgagc gcaaccgcac cttcgaggtg
    13681 gcggcgcgga cggagctgct gatcgtccac atggtcccga gcgaggcgac cgagctggcc
    13741 gacctgttcg gcggcgagac cggcgacgag gtcgacaagt tcgcccgctg cgactggcgg
    13801 cccggccccg gcggagcgcc ggtgctcgac ggctgcgaga actggttcgc gggccgggtg
    13861 ctggagcgct tcgcggtcgg cgaccaccac gcctacctgc tcgagccgtg cgcggcccac
    13921 agcggagacg accacggtca gttcaacttc catcgcgcga agcggatcga ggcgggacat
    13981 cccgcctagc ggcgatcgcc gcgactggga atcgccgggc cgggtaggag cccgtcatga
    14041 cagagcagaa agacgacatg aagcccggcg acgaggtccc gcccggcacc gacgcggccg
    14101 gtccagcgcc ctgcccgcag tgtggcgggg acggcaaggt cgatggcggg gagtgcgacc
    14161 tgtgtcgcgg gaccggcgag atcgagcagc ccgtcggcgg gggctgaggt ggactcgctc
    14221 cgttgggcgt cctcgaggac cggttcgcgc cgagcggttt gaggggcgct gtggctagta
    14281 gctggtgctg ctccgcgtga ctgtcggctg ggtagggcgg agtgcgggcg ggttggcggc
    14341 cgatcagtcg gctcggtcct tggccgtcga ggttctcagc agtactttca cccggggcgg
    14401 gctccgtgtc cgccgggaca gtgcataacg accgtctggc catcctcgta gcgcaccggc
    14461 gggattcctg cacctaggcc atgcggttcg accgcctaag ccccttgctc gcgggccgat
    14521 gggacctctg catgcggtgc cctggccccc cggagaccgc ccgccacccg cccgatccaa
    14581 gtcctcgaag acacccacag gaacaccgcc agccccatcc ctcaccctta tggtcccacc
    14641 gatggacgcc tgggtagcga gcgccgctga gcagatcgcg gcgcgagccg agcgcgacct
    14701 cgaggcgctg gtcgcggtct cttcgccgtc gggcgacgtc gagggcgcag aggagtgcat
    14761 cgcgctggtc ggcgcgctgg ctccgccgga ggcggagatc gaacggcgac cgtgctcgtc
    14821 gccggggcat gccgacgacg tgctgctgcg cctgggcggc agcggctcgg cgcggctact
    14881 gctgctcggc cacatcgaca ccgtcgtcgg ccacgccagc caccgaccgg tcgagcgcga
    14941 cggcgggcgc ctggtcggct cggggagcat cgacatgaag ggcggcgacg tgctcgcgct
    15001 cggggtcctg cgcgcgctcg ccgagcggac cgccgacttc gcggaggtcg cgctgctgct
    15061 cgtcaccgac gaggagtggc gctcggcgcc gttcgcccac gtcgaggcct tcgccggctg
    15121 ggacgcctgc ctctgcttcg aggcgggcca gcgcactgcc gagggcgagg aggcggtgat
    15181 cgtgcggcgc aaggcggcag cgacgctgcg cgtgagcgcg gccggccgct ccgcgcactc
    15241 cggctcggcg cccgagcgcg gccgcaacgc gctgctcgcg ctggctgccg ccgcgcaggc
    15301 ggtcgctgcg gccaatgacc ccgccggcgc cgacgagttg acggccgtcc cgacgatcct
    15361 gcgctccggc gatgccttca acgtcgtccc cggcgacggc gagctcttct gcgacctgcg
    15421 ctcgaaccgc accgacgcct tcgacgaggt gcgcgcgctc gtcccagacg aggtcggcgg
    15481 cgcgacgctc ggcacccagt tcatccgcct ctggccggca atggacgcgc gcgccgcgac
    15541 ggcgccgctg atcgccgcgg cggccgaccg cctccgccgg ccgatcgccg ccgccggccg
    15601 cggcggagcc agcgacgcca gccacttcgc cagcgtcgtc ccggtgacga tcgacggcct
    15661 cgggccacgc ggcggcggcg cgcacgccag cgacgagtac gtcctcgccg aatcgctcta
    15721 tccgcgtgcc gaggtcgccc tcgcgatcgt cgacgcggtg ctgaccagcg gcgcccgcta
    15781 gcgcctgtat cggcgccgcg ggccctcgat gaccgcgtcc gagtagtacg gcggcttgcc
    15841 gcccgcggcg gcaaggacgc ggcggcgcat ctctgcgacc acccgcggcc ggcgctcgga
    15901 cacgtcgacg tgctcgcccg ggtccgccct gaggtcgtag agccgctcgt tgcgtccgtc
    15961 gttttggacc tggtaggccc agcggtagtc gcgcacgtag gtgctgttgc cgtagccgcc
    16021 gacggcgtag ggccggtcct cgtccggcag gcccgcagtc agcaccgggg tcaggtcggc
    16081 gccctcgaag gtcgacgggc ggccgatccc agccagggac gccagcgtcg gcgccacgtc
    16141 gtgggtcgag gcgaaccagt tgctcgcctg gccggcgccc gcgccgccgg gctcgcgcag
    16201 caggagcggc acgtggatca gctcggggta gagcttgccc ggccccttcc cggtccagtt
    16261 gcgctcgccg aggaagaccc cgtgatcgct gaccaggacg atcgccgtcg agtcgtcgag
    16321 cccgagcttc cagagccggt cgaggaacag gccgagccag cggtcggtca tcgtgacggc
    16381 cgccttgtag gtcgtctgca ggcgggcgat ctgggccgct tcgaggtagt tcgaggcgta
    16441 ggtgtagccg acctcggcga tctcgtagcc gcggtagtcc gggtcgccgt acatgtctag
    16501 gtagtggcgc ggcgggatcc atggctcgtg ggggtcgaag ctgtcgacga ccatcaggaa
    16561 ctgcttctgg cgctttgcga tcggcagcag cttcgcggcc gaccggaaca cccgcgaggc
    16621 ggcctgctcg acctcgttgt ggccgcggcc gttgttggcc aggtactgct gaatcgacgc
    16681 gatccgccgc tcgttgcgca acggccccgg cggcagccgg cgcagcgccg ccgagcgcgg
    16741 aacgctcttg cgcggccggc gcgccccgcg ctggccgtgg actcgcacga agcggtcggg
    16801 cgtgcggcgg aacggctcga gcgcacgcgt gtagccgagg aacggattgt cggtcacata
    16861 ggacgtccac cagcccgccc gccgcagcgc ggtgatcagc gtctcggagc cgggggcgat
    16921 cggctgccag cccggccggc cggccatccc ctcccacggc cgccagccgc ggaacgggta
    16981 ggcgtggcgt ccggacatga tcgaccgccg cgccggcatc gtcggcatcg cctccgggaa
    17041 gacgtgcgtg aagcgcatgc tgccgcccgc aagctcgtcg atgttcggcg tccgcatccc
    17101 gcgcgcgccg tagcagcgca ggtgatcggc gcgcaggctg tcgaggacga tcagcacgac
    17161 gttgcggcgc tcgctggctg ccagcgcccg gctgatcgag cgccagccgc ctgacccgcc
    17221 ggcgacaccc agaccagcga ttccggcgac gccggctgcg ccgagcagct cgcgccggct
    17281 cagcccgccc tcgtcgtcct gccccgcccg ccgcgccatc ccgactgaca actcaggaga
    17341 accgctaaag gtgcgccgct accatccgat tctctctttg ccgtgttcgg ggggctcgga
    17401 ctcgcatgcg aacgcttctg cccatcctcg cctgcgccct gtgcgcagtt ctgctcgccg
    17461 cgccacgcgc ggacgggccg gcgcctgccg aggcgcaggt ggagagccaa gcgcggccga
    17521 acgtcgtcgt cctgatgacc gacgaccaga cgctgcgctc gatgcgcgcg atgccgcgca
    17581 cgcgtgcgct gatcggcgcc cagggcgcgc gcttcgccaa ctccttcgtc agcaacccgc
    17641 gctgctgtcc cgcgcgcgcc accttcctca ccggtcagta ctcgcacaac aacggcgtgc
    17701 tgaccaacgg cccgccgctc ggcggctggc agcggctgcg cggaacttcg aactggctgc
    17761 ctacatggct gcgcagcgcc ggctaccgca ccgtccacgt cggcaagttc ctcaaccact
    17821 acggcgtcga cgacccgcac gaggtcccgc ccggctggga cgagtggtac gggacgatcg
    17881 acccgacgac ctaccgctac tacggctaca ccgtcaacga gaacggggag ctgcgcacct
    17941 acggccgcga ccacgacccg cgctggtact cgaccgactt cgcctcgcgt cgctccgccg
    18001 ggatcgtcga gcggctcgcg ccggagaccc agccgttctt cctgtcggtc gccttcctgg
    18061 cgccgcacac aggcggcccg tacgaagccg gcgacccgac gtcgctcggc acgccggcgg
    18121 tcgccgagcg ccaccgcggc gcctacgcga gcctgccact gccgcggctg gccgccttcg
    18181 acgaggccga cgtctccgac aagccgccgt tcctgcgccg gccgcggttg aggccgcgcg
    18241 cgatcgccga catcgaggcc ctctaccgcc agcggctcga gtcgctgctc gcggtcgacg
    18301 aggggatcga gcagatcgtc gacgcgctgc gccgcagcgg cgagcttcac aacacgctga
    18361 tcgtcttcac ctccgacaac ggctacatgg ccggcgagca ccgcctcccg cacggcaagg
    18421 cgaaggccta cgacccctcg atccgcgtgc cgctgatgat ccgcggcccg ggcgtcccgg
    18481 tcggcgtcga gccgcagcag atggtcgtca acgccgacct cgcgccgacg atcctcgagg
    18541 cggcgcgcgc gaccgccggc cgcgtccagg acggccgctc gctgctgccg ctgacgcgcg
    18601 acccctccct cggcaccggc cgcccggtac tggtcgagat cgcgaagggg gccggcatga
    18661 agacgacggc gatccgcacg ccgcgctggc tctacgccga gcacgccggc ggttggagcg
    18721 agctctacga cgtcgttgcc gatccgcagc agctgcgcag cctgcacgcc gacccgacgc
    18781 tcgcgggggt gcgcgcgcgg ctcgccagcc agctggcggt actgcgcgcg tgcgcagggc
    18841 tgtcctgccg ccagccgttc gccgagcccg cgcgacgggc gcggcgacgt cacggcgcgg
    18901 gtactcggcg gctcggtaag cgacccgttc accgcaactc gcggtcgcct cgccggttct
    18961 agggctgagc ccagatcgga ggcgtcatgt tgaagcccac cgcgctcctc gcagcgctga
    19021 cccttgccat cgtcgcggcc gccgcagcgc cggcaggcgc gcagacggcg g
//
LOCUS       DATABV010000047        11830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1060421, whole genome shotgun sequence.
ACCESSION   DATABV010000047 DATABV010000000
VERSION     DATABV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060421"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VHF88_01960"
     CDS             complement(<1..389)
                     /locus_tag="VHF88_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEX2160565.1"
                     /translation="MEIVALFGPTGVGKTAVALELADRLRERGEDPVAVSCDALQVYE
                     GLSILTGTASAEEQSRLEHRLIGVVPLTKPFSLGDYMPRAHAEIDAALANGRRPIVVG
                     GTGLYLRAALASLSLEKVPPNTADSEL"
     gene            complement(393..1715)
                     /gene="miaB"
                     /locus_tag="VHF88_01965"
     CDS             complement(393..1715)
                     /gene="miaB"
                     /locus_tag="VHF88_01965"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX2160566.1"
                     /translation="MRKYHLTTFGCQMNEHDSERMKGMLDSLGYREAPERADADLILF
                     NTCSIREKADSRFVGHLGEAKRLKSEDPTRIVGVGGCWSQSMKEKVFEQFPFVDVAFG
                     PGQVHRLAEFLTSDNLTAQGYFEFEGFTGHLPAKRERDFQGWVQISVGCNCVCSYCIV
                     PSTRGREVSRPAGELIAEIERLAADGVREVTLLGQNVNSYGRDLPAGAQVSFAELLAM
                     VDAVDGIDRVRYTSPHPKDMREDVIRAHAELASVCPHIHLPLQSGSSRILKAMRRTYN
                     RQRYMDRVALIREIVPDCSITTDIIVGFPGESDADFEETMEVVEEVRYDSAFTFIFSP
                     RRGTLAAELEDQLPHDVKRERMERLVEAVQRHADERAQRFVGRTVEVLVEGPSRTDPS
                     KLRGRTTHNKTVNFEGLASPGETALVEIHSATSQTLAGQESLLARIGA"
     gene            complement(1827..3116)
                     /gene="rny"
                     /locus_tag="VHF88_01970"
     CDS             complement(1827..3116)
                     /gene="rny"
                     /locus_tag="VHF88_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX2160567.1"
                     /translation="MEILLAALVAAAVSGGVVLLAPRARRRLALAHSARAVGAPAVGR
                     SRERRLDELEQAAGMSVAEARAALLAELEDDLRHERARLIRQVEDETRHDAERRARSI
                     LATCMQRVASRHSAETTVTSIQLESDELKGRIIGREGRNIRALETLTGIDVIIDDTPS
                     TVVLSGFDGLRREVARLTLEKLLADGRIHPARIEEVYGQARSELDRRIVELGEEATLD
                     AGVGTAHPELVTLLGRLRFRTSYGQNVLDHSVECARLARLIAEELGGNAKVAARAALL
                     HDIGKAVSQEVDGPHALVGADLARRHGESDAVAHAIAAHHNEVEPQTVEAVIVQIVDA
                     LSGARPGARGDSLEHYVTRLHDLEGVAARHTGVEKVYAMQAGRELRVIVRPDEVDDDG
                     ATILSREIAREVERELEYPGQIKVTVIRESRAVDIAR"
     gene            complement(3242..3697)
                     /locus_tag="VHF88_01975"
     CDS             complement(3242..3697)
                     /locus_tag="VHF88_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX2160568.1"
                     /translation="MDEAQRALDLAFKALNRRERSVGELRAYLEGKRVEPDAIDAAVA
                     ELERAGLLDDAGFARRFAEDKRTLERWGAERIERELRRRGVARDLVEAAVAQQDRDGE
                     LVSALALLAEKVREPPADDRGRDRAWRMLVRKGYAPELAYDAVRAHMAG"
     gene            complement(3702..4790)
                     /gene="recA"
                     /locus_tag="VHF88_01980"
     CDS             complement(3702..4790)
                     /gene="recA"
                     /locus_tag="VHF88_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX2160569.1"
                     /translation="MDKDQEKAAKARDAALNSALAQIRKDHGEGAIMKMGDESAQVRV
                     SAIPTGALSLDLALGVGGLPRGRIVEVFGPESSGKTTLVYHVLAEAQKRGGICAFIDA
                     EHAMDPIYAKRIGVDVDELLVSQPDYGEQALEIADMLIRSGTIDVVAIDSVAALTPKV
                     ELEGQMGDQTVGLQARMMSQAMRKLAGNLNRANTLCLLTNQIREKIGVMFGSPETQPG
                     GRALKFYSSQRLDIRRIETLKEGTEAVGNRVRVKVVKNKVASPFRQAEFDIEYGLGIS
                     SAGCILDLGIEHDIVQKSGSFFSYGEQRLGQGRNNAKGFLRENPAVMEEIEQKIHAAV
                     GIAKDGPIAAVEPPAGVDPETGEVREAA"
     gene            complement(4881..6149)
                     /locus_tag="VHF88_01985"
     CDS             complement(4881..6149)
                     /locus_tag="VHF88_01985"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX2160570.1"
                     /translation="MSARAGVVVTGTEVLTGRVRDRNGPWLSDRLGELGIDLAHITIC
                     GDRPEDMEAQLRFLAGQGVDVIFTSGGLGPTADDVTLEIVARFTGRERSLDEALEQRI
                     AEILRPLMKRWPNLDLDAIRAANRKQALVPRGATVIEPAGTAPGMVVPAADGLPAVVV
                     LPGPPRELHAMWPAVLASDAFQAVLGRATAYEQAMLRLFGIPESEIAETLRVAEQQID
                     DFDALEITTCLRRGEVEVVTRYEAAAAPAWDALAALIAERHSDTLFSTDGSTVDEQVA
                     RLLGTDMVATAESCSGGLLAARLTERPGASDFVAGGVVAYSNAAKTALLGVDATLIER
                     HGAVSGEVAEAMADGALKRFEAQVAISVTGVAGPAGSDEKPVGTVFWCAKRAGGEGLV
                     RSARLPGDRAEIRDRSTTVGLHLLRRLLAA"
     gene            complement(6146..6949)
                     /locus_tag="VHF88_01990"
     CDS             complement(6146..6949)
                     /locus_tag="VHF88_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2160571.1"
                     /translation="MPPIGETLREARMRQRLDIADVEERTKIRAKYLRALENEEWGLL
                     PGPTFVKTFLRTYAEVVGVDPYLLVEEYRLTEERSDGPEFQPLAPMPARDRRDRGRGG
                     RRQPQRPPRRGGLIAGAVAVGLIGFLLLLGLLGGDSGDDDPPAERAATTETQPPTAQQ
                     RQRQRALQRRRRAGAKPRGVNVRIAPAEPTYVCVDSGPGTPVLFEDTLDAPRSFRNAR
                     QVRVNLGKRSARVALNGKPLTLDPSGDPIALDLTRDGSTELDATEAPCA"
     gene            complement(6979..9504)
                     /locus_tag="VHF88_01995"
     CDS             complement(6979..9504)
                     /locus_tag="VHF88_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX2160572.1"
                     /translation="MSRAKSTRKSPNRKPARKRSPARRAGGGLGLPAIEQHHRDLIGL
                     GLVAFGCFLAVVLYGGETGGRVGEVAADALRFLLGGVAYLAPVGFVAGGVLLVVDDVL
                     PSLRPFRAGAVCLTLGLALGLSAGSLGLGPGAASHSPLLESSYVSARGGVLGELLFSA
                     SSALFSEVGAHILFLFLMTGGVLLLTGASLASILRATGAGVATTTRVVRQSTSDLTSV
                     LSGERGPLSHAARRAEPAALPAAATAVDGPPEPPGVEPVVHATHVEAPALDAEDRFPD
                     LFADDEDLEQRVDAAEDAAADLADDEPFEAAAGVEAIAQDEDRAGETADLPAVEPVQE
                     PLTPMGNRRTDGVTEADDVDYALPSESFLKRSPTGRKADTAAIDKVGAQLVEALGHFG
                     VEAKVVGSVSGPHVTRYELRLAPGIKMSKVSNMRDDLAYALAADDVRILAPIPGKRAV
                     GVEVPNTVRHMVHLGDVSQDPPDGWSPLAVWLGKDIDGRAIGIDLAQQPHVLVAGTTG
                     AGKSGAVNAMLASILMRSSPNDVRLVLVDPKQVELNLYEQIPHLLTPVVTSPRLAANV
                     LQNLIGEMEQRYSLMSKARTRKLEELNRMRVRNGERPLPYVLCVIDELADLMMIAPGE
                     VEDAIIRLAQKSRAVGIHLLLATQRPSADIITGMIKANVPARIAFAVSSQTDSRVILD
                     QNGAESLLGKGDMLFKPGNGSKLNRIQGAFVDEEEIEKLTAHWRRQGEPELHEELLEA
                     VESEDGDDDRGDYDPDQDELLAEAIRTVVQMDTASTSMLQRRLRVGYTRAGRLIDMLE
                     RRGVISGYEGSKARNVLIGEAELPRVLAALEDPASAEPVAAPD"
     gene            9560..10243
                     /locus_tag="VHF88_02000"
     CDS             9560..10243
                     /locus_tag="VHF88_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2160573.1"
                     /translation="MQRSPRVLVVEDDRDIADVLRRSLDLDGYEVRLAGDGEAALAEA
                     DAFEPDAVILDLGLPKLDGVEVCRRLRSDGDVPILMLTARDGVDARVDGLDSGADDYL
                     VKPFERRELLARLRALLRRRPPRGSASLVVGDLKLNPDAREALRGDRELDLTSREFEL
                     LEYFMRNERLVVSRQRLLDEVWGYHPFAETNTVDVFISNLRRKLEAGGEPRVLHTIRG
                     AGYVLKDAA"
     gene            10240..11781
                     /locus_tag="VHF88_02005"
     CDS             10240..11781
                     /locus_tag="VHF88_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2160574.1"
                     /translation="MRGPIAGARLRLPIRLKLAAVSGGLTFAILLAFALVVGGFTERK
                     LQSSFDSELRETAADLQSRLQVNGVGQVEPELFRFAAAGGAVLRLHDRVNNRVYAISG
                     TPKLGPLYEGIRDVGRYRVVSRPLFVRSLGTTGTFQTVPRPLDGAVAAIQYGKPTDNL
                     NATIDRVRFFLLLGVLIGTVFAFLAGFFVARRAMRPISGLTKAAREVARTRDPARTVL
                     PRPVANDEVAELSTTLSEMLAELAAARSETEASLERQRQFVADASHELRTPLTSILAN
                     LELLQEELDRPGGPRSGSAPSQIADSALRSSQRMRRLVGDLLLLARADAGRREQPRPI
                     DLAEVVREAAREAAAVGGDHPLTLDLPTAAGTAVVEGARDDLHRLVLNLIENAFLHTP
                     AGTPVVASVQRPTPSGGTGGDRIRLQVADRGPGVPREQRERIFERFARSEGDRSAVRG
                     TGLGLAIVRAVAEAHGGSAAVRDADGGGALFEVELPAALRSSPTRVEDRDTDPSTVAK
                     GAARP"
BASE COUNT         1585 a         4318 c         4165 g         1762 t
ORIGIN      
        1 cagagctcgg agtccgcggt attcggcggc accttctcga gcgagaggct cgccagcgcc
       61 gcgcgcaggt agagcccggt gccgccgacg acgatcggcc gcctgccgtt ggccagggcg
      121 gcgtcgatct ccgcgtgggc gcgcggcatg tagtcaccga gcgagaacgg cttggtaagc
      181 gggacgacgc cgatcaggcg gtgctccagg cgactctgtt cctcggcgct cgcggtgccg
      241 gtgagaatcg acagcccctc gtagacctgc agcgcgtcgc aggagacggc taccggatcc
      301 tcgccccgct cgcgcaggcg gtcggccagc tccagcgcga ccgcggtctt cccgaccccg
      361 gtcggcccga aaagcgcgac gatttccacc gatcaggcgc cgatgcgggc gagcagcgac
      421 tcctggccgg cgagcgtttg gctggtcgcc gagtggatct cgacgagggc cgtctccccc
      481 gggctcgcca gtccctcgaa gttgacggtc ttgttgtgcg tcgtccttcc gcgcagcttg
      541 gaggggtccg tccgcgacgg tccctcgacc agcacctcga cggttcgccc gacgaagcgc
      601 tgcgcccgtt cgtcggcgtg ccgctgcacg gcctcgacca ggcgctccat ccgctcgcgc
      661 tttacgtcgt gcggcagctg gtcttccagc tcggccgcca gcgtcccgcg gcgcggggag
      721 aatatgaacg tgaacgccga gtcgtagcgg acctcctcga cgacctccat cgtctcctcg
      781 aagtcggcat cgctctcgcc cgggaagccg acgatgatgt ccgtcgtgat cgagcagtcc
      841 ggcacgatct cgcggatcag ggcgacgcgg tccatgtagc gctggcggtt gtaggtgcgg
      901 cgcatcgcct tgaggatgcg gctcgacccc gactgcagcg gcaagtggat gtgcgggcag
      961 acgctcgcca gctcggcatg ggcgcggatg acgtcctcgc gcatgtcctt ggggtgcggg
     1021 ctggtgtagc ggacgcggtc gatgccgtcg accgcgtcga ccatcgccag cagctccgcg
     1081 aagctgacct gggctccggc cggcaggtcg cgcccgtacg agttgacgtt ctggccgagc
     1141 agggtcacct cgcgcacgcc gtcggccgcg aggcgctcga tctccgcgat cagctcgccg
     1201 gcgggccgcg agacctcacg cccgcgcgtc gaggggacga tgcagtagga gcagacgcag
     1261 ttgcagccga ccgagatctg gacccagccc tggaagtcgc gctcgcgctt ggcgggcagg
     1321 tggccggtga agccctcgaa ctcgaagtag ccctgggcgg tcaggttgtc gctggtcagg
     1381 aactccgcga gccggtggac ctggccgggc ccgaacgcga cgtcgacgaa cgggaactgc
     1441 tcgaagacct tctccttcat cgactgcgac cagcagccgc cgacaccgac gatccgcgtc
     1501 ggatcctcgc tcttgagccg cttcgcctcg ccgagatgcc cgacgaagcg gctgtcggcc
     1561 ttctcgcgga tcgagcaggt gttgaagagg atcaggtccg cgtccgcgcg ctccggcgcc
     1621 tcgcggtagc ccagcgagtc gagcatcccc ttcatccgct cggagtcgtg ctcgttcatc
     1681 tggcagccga acgtcgtgag gtggtacttc ctcatgtcga aaaggtaccg acctcagagg
     1741 gtatgaccgc cggcctggcc ggcacccggc caaggaccga tggggggctc ggggggcgcg
     1801 agccccccgg cctcatccag atccgctcac ctcgctatgt ccacagcacg gctctcgcgg
     1861 atcacggtga ccttgatctg gcccgggtac tcgagctcgc gctcgacctc gcgggcgatc
     1921 tcacgcgaga ggatcgtggc gccgtcgtcg tcgacctcgt ccggcctgac gatcacgcgc
     1981 agctcgcggc ctgcctgcat cgcgtagacc ttctcgaccc ctgtgtgacg ggcggcgacg
     2041 ccctcgaggt cgtgcaggcg cgtcacgtag tgctcgagcg agtcgccgcg ggcgccggga
     2101 cgcgcgccgg agagtgcgtc gacgatctgc acgatcaccg cctcgacggt ctggggctcg
     2161 acctcgttgt ggtgcgcggc gatcgcgtgg gcgacggcgt cggactcgcc gtggcgccga
     2221 gccaggtccg caccgacgag cgcgtgcggg ccgtcgacct cctgcgacac cgccttgccg
     2281 atgtcgtgca gcagcgccgc gcgcgcggcg accttggcgt ttccgcccag ctcctcggcg
     2341 atcagccttg cgaggcgggc acactcgacc gagtgatcga ggacgttctg tccatagctg
     2401 gtccggaagc ggagccggcc cagcagcgtg acgagctccg gatgggcggt tccgacgccg
     2461 gcgtcgagcg tcgcctcttc gccgagctcg acgatgcgcc ggtcgagctc cgagcgcgcc
     2521 tggccataga cctcctcgat cctcgcggga tggatgcggc cgtcggcaag gagcttctcg
     2581 agcgtcagac gggcgacctc gcgtcgcagg ccgtcgaagc ccgagagcac gacggtgctc
     2641 ggcgtgtcgt cgatgatcac gtcgatgccc gtcagcgtct cgagcgcacg gatgtttcgc
     2701 ccctcgcggc cgatgatccg tcccttcagc tcgtcggact cgagctggat cgacgtgacg
     2761 gtggtttccg ccgagtggcg gctggcgaca cgctgcatgc acgtcgcgag gatgctgcga
     2821 gcgcggcgct cggcgtcatg gcgggtctcg tcctctacct ggcggatcag ccgtgcgcgc
     2881 tcgtggcgca gatcgtcctc gagctcggcg agcagagcag cgcgcgcctc ggcgacgctc
     2941 atgcccgcgg cctgctcgag ctcgtctagg cgacgctcgc gactccgccc aaccgcaggc
     3001 gcccccaccg cacgagcgct gtgggcgagc gcgagccgtc gccgcgctcg tggcgccagc
     3061 agcacgaccc ctcccgacac agccgccgcg accagcgccg cgagcaggat ctccatctca
     3121 cagtctcctt agcgtcgacc agatacggcc gctcggagac caggttagtg acccggcgaa
     3181 ccccccgcaa acacaggctt accgaaagga taagcccgtg tagagccgta cgccaatgtg
     3241 tctagcctgc catatgcgcg cggacggcgt catacgccag ctccggcgcg tagcccttgc
     3301 ggacgagcat ccgccatgcg cggtcgcggc cgcggtcgtc ggccggcggc tcgcgcacct
     3361 tctcggccag cagcgcgagc gccgacacga gctcaccgtc gcggtcctgc tgggcgacgg
     3421 ctgcctccac gagatcgcgc gcaacccccc gacggcgcag ctcgcgctcg atccgctccg
     3481 ctccccagcg ctcgagcgtt cgcttgtcct cggcgaagcg gcgtgcgaag ccggcgtcgt
     3541 cgagcagtcc ggcccgttcg agctcggcta cggccgcgtc gatcgcgtcg ggctcgacgc
     3601 gcttgccctc gagataggcc cgcagctcgc ccacgctgcg ctcgcgccgg ttcagcgcct
     3661 tgaacgccag gtcgagcgcg cgctgcgcct cgtccattgc gctaagcggc ctcgcggacc
     3721 tcacccgtct cggggtcgac gccggcgggc ggctcgaccg cggcgatcgg gccgtccttc
     3781 gcgatgccga ccgcggcgtg gatcttctgc tcgatctcct ccatcaccgc cgggttctcg
     3841 cgcaggaacc ccttggcgtt gttgcgcccc tggccgagcc gctgctcgcc gtaggagaag
     3901 aacgagccgg acttctggac gatgtcgtgc tcgatcccca ggtcgaggat gcagcccgcc
     3961 gacgagatgc cgagcccgta ctcgatgtcg aactcggcct ggcggaacgg cgatgcgacc
     4021 ttgttcttga cgaccttgac gcggacgcgg ttgccaactg cctcggtgcc ctccttgagc
     4081 gtctcgatcc gccggatgtc gagccgctgg gacgagtaga acttcagcgc gcggccgccc
     4141 ggctgggtct ccggcgagcc gaacatcacg ccgatcttct cgcggatctg attcgtcagc
     4201 aggcagagcg tgttggcgcg gttgaggttg ccggccagct tgcgcatcgc ctggctcatc
     4261 atccgcgcct ggagcccgac cgtctggtcg cccatctgcc cctcgagctc gaccttcggc
     4321 gtcagcgcgg cgaccgagtc gatcgcgacg acgtcgatcg tgcccgagcg gatcagcatg
     4381 tccgcgatct cgagcgcctg ctcgccgtag tcgggctgcg agaccagcag ctcgtcgacg
     4441 tcgacgccga tccgcttggc gtagatcggg tccatcgcgt gctcggcgtc gatgaaggcg
     4501 cagatcccgc cgcgcttctg ggcctcggcg agcacgtggt agacgagcgt cgtcttgccc
     4561 gaggactccg gtccgaagac ctcgacgatc cgcccgcgcg ggaggccgcc gacgccgagc
     4621 gccaggtcca gcgacagagc gccggtcggg atcgccgaga cgcgcacctg ggcgctctcg
     4681 tcgcccatct tcatgatcgc cccctcgccg tgatccttgc ggatctgcgc cagcgcgctg
     4741 ttcaacgcgg cgtcgcgggc cttggcggcc ttctcctggt ccttgtccat ggcggtctcc
     4801 tctaagtccg ttagggatac gaggctaggt agccaaccgg acggttagag acggacgacc
     4861 gtggttctgc aacgtctatg tcacgcggcg agcagcctgc gcagcaggtg cagcccgacc
     4921 gtcgtcgacc ggtcgcggat ctcggcgcgg tcgccgggca gccgcgcgct gcgcacgagc
     4981 ccctcgccgc cggcgcgctt ggcgcaccag aacaccgtcc cgaccggctt ctcgtcgctg
     5041 ccggcgggtc ccgcgacgcc ggtgaccgag atcgcgacct gggcctcgaa gcgcttcagc
     5101 gccccatcgg ccatcgcctc ggcgacctct cccgagaccg cgccgtggcg ctcgatcagc
     5161 gtcgcgtcga cccccaacag cgccgtcttg gccgcgttgg agtaggcgac gacgccgccc
     5221 gcgacgaagt ccgaggcgcc ggggcgctcg gtcaggcgcg ccgccagcag acccccgctg
     5281 cacgactccg ccgtcgccac catgtccgtc ccgagcagtc gcgccacctg ctcgtcgacg
     5341 gtcgagccgt cggtcgagaa gagcgtgtcg gagtggcgct cggcgatcag cgcggcgagc
     5401 gcatcccagg ccggcgcggc ggccgcctcg tagcgggtca ccacctcgac ctcgccgcgc
     5461 cgcaggcagg tcgtgatctc gagggcgtcg aagtcgtcga tctgctgctc ggcgacgcgc
     5521 agcgtctcag cgatctccga ctcggggatc ccgaacagcc gcagcatcgc ctgctcgtac
     5581 gccgtcgccc tcccgagcac cgcctggaag gcatcggacg cgagcacggc gggccacatc
     5641 gcatgcagct cgcgcggcgg cccgggcaac acgacgaccg cgggcagccc gtccgctgcc
     5701 ggcacgacca tccccggtgc tgtgccggcc ggctcgatga cggtcgcgcc tctcggaacg
     5761 agcgcctgct ttcggttcgc cgcccggatc gcgtcgaggt cgaggttcgg ccagcgcttc
     5821 atcagcggcc gcaggatctc ggcgatccgc tgttcgagcg cctcgtcgag cgaccgctcg
     5881 cggccggtga agcgggcgac gatctcgagc gtcacgtcgt cggcggtcgg gccgaggccg
     5941 ccgctggtga agatcacgtc gacgccctgc cccgcgagga agcgcagctg ggcctccatg
     6001 tcctcagggc ggtcgccgca gatcgtgatg tgggcgaggt cgatgccgag ctcgccgagc
     6061 cggtctgaca gccacggccc gttgcggtcg cgcacgcggc cggtcaggac ctccgtgccg
     6121 gtcaccacca cgccggcgcg ggcgctcatg cgcagggggc ctcggtcgcg tcgagctcgg
     6181 tcgagccgtc gcgggtgagg tcgagggcga tcgggtcgcc gctcggatcg agagtcagcg
     6241 gcttgccgtt gagcgccacc ctggccgacc gcttgccgag gttgaccctc acctggcgag
     6301 cattgcggaa gctgcgcggc gcgtcgagcg tgtcctcgaa caggaccggc gtgccgggcc
     6361 cgctgtcgac gcagacgtag gtgggctcgg cgggcgcgat gcgcacgttg actccgcgcg
     6421 gtttggcgcc ggcgcgccgg cggcgctgca gggcgcgctg gcgctggcgc tgctgggcgg
     6481 tcggcggctg ggtctcggta gtcgccgcgc gctcggcggg cgggtcgtcg tcgcccgaat
     6541 cgccaccgag cagtccgagc agcagcagga agccgatcag cccgaccgcg accgcgccgg
     6601 cgatcagccc tccgcggcgc ggcggccgct gcggctggcg ccgtccgcca cggcccctgt
     6661 cgcgccggtc gcgcgccggc atcggggcca gcggctggaa ctcgggaccg tcgctgcgct
     6721 cctcggtcag gcggtactcc tcgaccagca gataggggtc gacgccgacc acctcggcgt
     6781 aggtgcgcag gaacgtcttg acgaacgttg ggccgggcag cagcccccac tcctcgttct
     6841 cgagcgcgcg caggtacttc gcacggatct tcgtgcgctc ctcgacatcc gcgatgtcca
     6901 ggcgctggcg catgcgcgcc tcgcgcagcg tctcaccgat cggaggcatc agtgcatcct
     6961 acggccggag cgcgaccgtc aatcgggcgc ggcgacgggc tcggcactcg cggggtcctc
     7021 tagcgcagcg agcacgcgcg gcagctcggc ctcgccgatc agcacgttgc gcgccttgga
     7081 gccctcgtag ccggagatca ctccccgccg ctcgagcatg tcgatcagcc ggccggcgcg
     7141 cgtgtagccg acgcgcaaac ggcgttgcag catcgaggtc gaggcggtgt ccatctggac
     7201 gacggtgcgg atcgcctccg caagcagctc gtcctggtcg gggtcgtagt cacctcggtc
     7261 gtcgtcgccg tcctcgctct cgaccgcctc gagcagctcc tcgtgcagct cgggctcgcc
     7321 ctggcggcgc cagtgggcgg tcagcttctc gatctcctcc tcgtcgacga aggcgccctg
     7381 gatccggttg agcttcgagc cgttgccggg cttgaacagc atgtcgccct tgccgagcag
     7441 cgactcggcg ccgttctggt cgaggatcac gcgcgagtcg gtctgcgagg agaccgcgaa
     7501 ggcgatccgc gcgggcacgt tcgccttgat catcccggtg atgatgtccg cgctcggccg
     7561 ctgcgtcgcg agcaggaggt ggatgccgac ggcgcgcgac ttctgcgcca gccggatgat
     7621 cgcgtcctcg acctcgcccg gggcgatcat catcaggtcg gcgagctcgt cgatcacgca
     7681 gagcacgtac ggcagcggcc gctcgccatt gcgcacccgc atccggttga gctcctcgag
     7741 cttgcgcgtg cgcgccttgc tcatcagcga gtagcgctgc tccatctcgc cgatcaggtt
     7801 ctgcagcacg ttggcggcga ggcgcgggct ggtgacgacc ggcgtcagca ggtgcgggat
     7861 ctgctcgtag aggttgagct cgacttgctt cgggtcgacg agcacgagcc gcacgtcatt
     7921 cggcgacgag cgcatcagga tcgaggcgag catcgcgttg acggcgccgg acttgccggc
     7981 accggtcgtg cccgcgacga gcacgtgcgg ctgctgggcg aggtcgatcc cgatcgctcg
     8041 gccgtcgatg tccttgccga gccataccgc cagcggcgac cagccgtcgg gcgggtcctg
     8101 cgacacgtcg ccgaggtgga ccatgtggcg gacggtgttc gggacctcga cgccgacggc
     8161 gcgcttgccg gggatcggag cgaggatccg gacgtcgtcg gcggcgagcg cgtaggcgag
     8221 gtcgtcgcgc atgttcgaga ccttcgacat cttgatcccg ggcgcgagtc gcagctcgta
     8281 gcgggtgacg tgcgggccgc tgacgctgcc gacgaccttg gcctcgacgc cgaagtgccc
     8341 gagcgcctcg acgagctggg cgcctacctt gtcgatcgcc gccgtgtcgg ccttgcggcc
     8401 ggtcggcgag cgcttgagga agctctcgct cgggagcgcg tagtcgacgt cgtcggcctc
     8461 ggtgacgccg tcggtgcgcc gattgcccat cggcgtcagc ggctcctgga cgggctcgac
     8521 ggccggcagg tcggccgtct ctcccgcccg gtcctcgtcc tgggcgatcg cctcgacgcc
     8581 ggcggcggcc tcgaagggct cgtcgtcggc caggtcggcg gctgcgtcct cagccgcgtc
     8641 gacgcgctgc tcgaggtcct cgtcgtcggc gaagagatcc gggaagcggt cctcggcgtc
     8701 gagcgcggga gcctcgacgt gggtggcgtg gacgaccggc tcgaccccgg gcggctccgg
     8761 cggaccgtcg acggcggtgg cggctgcggg cagcgccgcc ggctcggccc tgcgggcggc
     8821 gtggctcagc gggccccgct cgccggatag caccgacgtc aggtcggagg tcgactggcg
     8881 caccacgcgg gtggtcgtcg cgacgccggc accggtcgcg cgcaggatgc tcgccagcga
     8941 cgcgccggtg agcagcagga cgccgccggt catcaggaac aggaagagga tgtgcgcgcc
     9001 gacctccgag aacagcgcgc tcgaagcgga gaacaacagc tcgccgagca cgccgccgcg
     9061 cgccgagacg taggaggact cgagcagcgg ggaatgcgac gcggctcccg ggccgagtcc
     9121 gagcgagccc gccgagagcc ccagcgccag gccgagggtg aggcagaccg cccccgcgcg
     9181 aaacggccgc agcgaaggaa gcacgtcgtc gacgaccagc agcacgccgc ccgcgacgaa
     9241 gccgaccggc gccaggtagg cgacgccccc gagcaggaag cgcagcgcgt cggcggcgac
     9301 ctcgccgacc cgcccgccgg tctcgccgcc gtagaggacg acggccagga agcagccgaa
     9361 cgcgaccagg ccgaggccga tcaggtcgcg atggtgctgc tcgatcgccg gcaggcccag
     9421 cccgcctccc gctcgccgtg ctggcgaccg cttgcgcgcc ggcttgcgat tggggctctt
     9481 tcgtgtgctc tttgcgcgtg acatggctga attcggtcct tcgacggatc gagtccctcc
     9541 cctccctacc atcaggccca tgcagcgctc gccgagggtc ctcgtcgtcg aggacgaccg
     9601 cgacatcgcg gacgtcctgc gccgctcgct cgacctcgac ggctacgagg tccggcttgc
     9661 cggcgacggc gaggcggcgc tcgccgaggc ggacgccttc gagcccgatg ccgtgatcct
     9721 cgacctgggg ctgccgaagc tcgacggcgt cgaggtctgt cgccggctgc gctccgacgg
     9781 cgacgtgccg atcctgatgc tgaccgctcg cgacggcgtc gacgcccgcg tcgacggcct
     9841 cgactcgggc gccgacgact acctcgtcaa gccgttcgag cgccgcgagc tgctcgcccg
     9901 cctgcgcgcg ctgctgcgcc gccggccgcc gcgcggcagc gcgtcgctgg tggttggcga
     9961 cctcaagctg aaccccgacg cccgcgaggc cctgcgcgga gatcgcgagc tcgacctgac
    10021 cagccgcgag ttcgagctgc tcgagtactt catgcgcaac gagcgcctcg tcgtctcgcg
    10081 ccagcggctg ctcgacgagg tctggggcta ccacccgttc gccgagacga acaccgtcga
    10141 cgtcttcata tccaacctgc gccgcaagct cgaggccggc ggcgagccac gcgtgctgca
    10201 caccattcgc ggtgccggct acgtcctcaa ggacgcggca tgaggggccc gatcgccggc
    10261 gcgcggctgc gactgccgat ccggttgaag ctggcggccg tctcgggcgg gctgaccttc
    10321 gcgatcctgc tcgccttcgc gctcgtcgtc ggcgggttca ccgagcgcaa gctgcagtcg
    10381 agcttcgaca gcgagctgcg cgagaccgcc gccgacctgc agagccgcct gcaggtcaac
    10441 ggcgtcggtc aggtcgagcc cgagctgttc cgcttcgccg cggcgggcgg cgcggtgcta
    10501 cggctgcacg accgcgtcaa caaccgcgtc tacgcgatct ccggcacccc gaagctcggc
    10561 ccgctgtacg aggggatccg cgacgtcggc cgctaccgcg tggtgtcacg cccgctgttc
    10621 gtccgctcgc tcggcacaac cggcaccttc cagaccgtgc cccgaccgct ggacggcgcg
    10681 gttgccgcga tccagtacgg caagccgacc gacaacctca acgcgacgat cgatcgcgtg
    10741 cgcttcttcc tactgctcgg cgtattgatc ggaaccgtct tcgcgttcct cgcgggcttc
    10801 ttcgtggcac gccgcgcgat gcgcccgatc tccggcttga cgaaggcagc ccgcgaggtc
    10861 gcccgaacgc gcgacccggc ccgcaccgtg ctgccgcgtc cggtcgccaa cgacgaggtc
    10921 gccgagctct cgacgacgct cagcgagatg ctcgccgagc tcgcggctgc gcgcagcgag
    10981 accgaggcct cgctcgagcg ccagcgccag ttcgtcgccg acgcctcgca cgagctgcga
    11041 acgccgctca ccagcatcct cgccaacctc gagctgctcc aggaggagct cgaccgcccc
    11101 ggcggcccgc gctcgggatc cgcgccgagc cagatcgccg actcggcgct gcgctcgtcg
    11161 cagcgcatgc gacggctcgt cggggacctg ctgctgctgg cgcgcgccga cgccggccgg
    11221 cgcgagcagc cacgaccgat cgacctcgcc gaggtggttc gcgaggccgc ccgcgaggcc
    11281 gccgcggtcg gcggcgacca tccgttgacg ctcgacctcc cgaccgctgc cggcacggcc
    11341 gtcgtcgagg gcgcgcgcga cgacctccac cgactcgtcc tcaacctgat cgagaacgcc
    11401 ttcctgcaca cacccgccgg gacgccggtc gtcgcgtccg tgcagcggcc aaccccctcc
    11461 gggggcacgg gcggcgaccg catccgcctg caggtcgccg accgcggccc gggcgtgccg
    11521 cgcgagcagc gcgagcggat cttcgagcgc tttgcgcgca gcgagggcga ccgcagcgcc
    11581 gtgcgcggca cgggactcgg cctggcgatc gtgcgtgcgg tcgccgaggc gcacggcggc
    11641 agcgccgccg tccgggacgc cgacggcggc ggcgcgctgt tcgaggtcga gctgccggcg
    11701 gcgctccgat cgtcgcctac acgtgttgag gaccgcgaca ccgacccgtc caccgtcgcc
    11761 aagggagccg ctcgcccgtg agaaagcatc tgagacctgc cagtcgccgc cggggggccc
    11821 gggggggggg
//
LOCUS       DATABV010000048         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_980396, whole genome shotgun sequence.
ACCESSION   DATABV010000048 DATABV010000000
VERSION     DATABV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980396"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VHF88_02010"
     CDS             <1..1270
                     /locus_tag="VHF88_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2160575.1"
                     /translation="GVAEGTQDRLDLLTLDPDITSRQLDRRVAAIANAIDARVTLFGI
                     QQALGRGPGGFYVITDSRPDPTVPENRPLVRAAVRNGELALGETRHRGEPLAQVAQPL
                     LFEGEPSRVALYSRSLEDVVDTVSFVRSRMLIGGAIALLLALLGGAVVAQTLARRVRR
                     LEVAAEDVAAGQYVEPLEDPSEDELGELTRAFNAMQEQLRRVDDARREFISTASHELR
                     TPIFSLGGFVELLRDDDVSEEERRDFLDEIAGQVARLQKLAVDLLDLSRLDSGSLALD
                     LDTVDLAEVARLVAGEFKPATIEHGTDFQLQLPESGVPARCDRERVVQIVRILLDNAL
                     RHTPAGTTVRMSAGVDSAGAALTVADEGPGLPADTPVFDRFVTGGASQGAGLGLAIAR
                     ELAERMGGALRASENGRGASFTLELPDAPG"
     gene            1305..1679
                     /locus_tag="VHF88_02015"
     CDS             1305..1679
                     /locus_tag="VHF88_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160576.1"
                     /translation="MPAPSPNSRDVHLSVPARPDYLVLARLALSAMCRLTPLEHAEVA
                     DLKLAITEAASRFVADGQPLENVVNFDFRLLDDRIEMDLDGAPVDTPADEHEVSRAIV
                     EATVDDFQLQDGHVRLVKRFTA"
     gene            1696..>2846
                     /locus_tag="VHF88_02020"
     CDS             1696..>2846
                     /locus_tag="VHF88_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX2160577.1"
                     /translation="MTPADPKLVLVSNRGPATFERDEQGELIASRGGGGLVTALTGLV
                     HHRDALWVASAMSDEDAEMAAQHDSKSFSCEIEGVAYRVRLVASEAEAYDRFYNVIAN
                     PMLWFVQHYLWDLSNAPDIGRWEVEAYEHGYLTVNRDIADAVLEEIEGVEDPVVMVHD
                     YHLYAAPKFIRAARPDVFLHHFVHIPWTQPDAWRVLPRDMREDVFEGILANDIVAFHT
                     RAYRRNFLLCCRELFDLDVDERAGVVRVDGRDVWVRAYPLPISADTFRRTAQRPAVHE
                     YEREILRRRREHLILRVDRADLSKNILRGFSAFDLFLEQHPEFKEEVTFIAHLIPSRQ
                     DVPQYAEYLERIEALVEEEVEGREPAQDVLRQVGAVDAQDQVLAPAAQDL"
BASE COUNT          405 a         1070 c          960 g          411 t
ORIGIN      
        1 gggcgtcgcc gagggcaccc aggaccgcct cgacctgctc acgctcgacc cggacatcac
       61 ctcgcggcag ctcgaccggc gtgtcgccgc gatcgccaac gcgatcgacg cgcgcgtgac
      121 gctgttcggc atccagcagg cgctcggccg cggccccggc ggcttctacg tgatcaccga
      181 ctcccgcccc gacccgaccg tgccggagaa ccggccgttg gtgcgcgcgg cggtccgcaa
      241 cggcgagctc gcgctcggtg agacgcgcca ccgcggcgag ccgctcgccc aagtcgccca
      301 gccgctgctc ttcgagggcg agccctcgcg cgtcgctctc tactcgcgct cgctcgagga
      361 cgtggtcgac accgtcagct tcgttcgcag ccggatgctg atcggcggcg cgatcgcgct
      421 gctgctggcg ctgctcggcg gcgcggtggt cgcgcagacg ctggcgcgcc gggtgcggag
      481 gctggaggtc gccgccgagg acgtcgccgc cgggcagtac gtcgagccgc tcgaggaccc
      541 ctcggaggac gagctcggcg agctgacgcg cgccttcaac gcgatgcagg agcagctgcg
      601 ccgggtcgac gacgcgcggc gcgagttcat ctcgaccgcc tcgcacgagc tgcgcacgcc
      661 gatcttctcg ctcggcgggt tcgtcgagct gctgcgcgac gacgacgtca gcgaagagga
      721 gcggcgcgac ttcctcgacg agatcgccgg ccaggtcgcg cggctgcaga agctcgccgt
      781 cgacttgctg gacctgtcgc ggctcgacag cgggtcgctc gcgctcgacc tcgacaccgt
      841 cgacctcgcc gaggtcgcgc ggctggtcgc cggggagttc aagccggcga cgatcgagca
      901 tgggacggat ttccagctcc agctgcccga gagcggcgtg ccggcgcgct gtgaccgcga
      961 gcgggtcgtc cagatcgtgc ggatcctgct cgacaacgcg ctgcgccaca cgcccgccgg
     1021 caccaccgtg cggatgagcg ccggcgtcga ctcggccggc gcggcgctga ccgtcgccga
     1081 cgaggggccc ggcctgcccg ccgacacgcc cgtcttcgac cgcttcgtga ccggcggcgc
     1141 cagccagggc gccggtctcg ggctggcgat cgcccgcgag ctggccgaac ggatgggcgg
     1201 cgcgctgcgc gccagcgaga acgggcgcgg ggccagcttc acgctcgagc tgcccgacgc
     1261 gcctggttga tgcggaacgg ccctttcagg gtaggttgaa ggacgtgccg gcgccgagcc
     1321 cgaacagtcg cgacgtccac ctgtccgtgc ccgcgcggcc cgactacctc gtcttggcgc
     1381 ggctcgccct atccgcgatg tgccgcctga cgccgctcga gcacgccgag gtcgccgacc
     1441 tcaagctcgc gatcaccgag gccgcgtcgc gcttcgtcgc cgacggccag ccgctcgaga
     1501 acgtcgtcaa cttcgacttc cgcctgctcg acgaccggat cgagatggac ctcgacggcg
     1561 cccccgtcga cacgccggcc gacgagcacg aggtatcgcg ggcgatcgtc gaggcgacgg
     1621 tcgatgactt ccagctccag gacggtcacg tcaggctcgt caagcgattc acggcttgac
     1681 gactctccag cgccaatgac cccggcggac ccgaagcttg tcctggtctc taaccgcggc
     1741 cctgcgacgt tcgagcgcga cgagcagggc gagctgatcg ccagccgcgg cggcggcggc
     1801 ctcgtcaccg cgctcaccgg cctcgtccac caccgcgacg cgctctgggt cgcctcggcg
     1861 atgtccgacg aggacgccga gatggccgcc cagcacgaca gcaagagctt ctcgtgcgag
     1921 atcgagggcg tcgcctaccg cgtgcggctc gtcgcctccg aggccgaggc ctacgaccgc
     1981 ttctacaacg tgatcgccaa cccgatgctg tggttcgtcc agcactacct ctgggacctc
     2041 tccaacgccc ccgacatcgg ccgctgggag gtcgaggcct acgagcacgg ctacctgacc
     2101 gtcaaccgcg acatcgccga cgccgtgctc gaggagatcg aaggcgtcga ggacccggtc
     2161 gtgatggtcc acgactatca cctctacgcc gcgccgaagt tcatccgggc tgctcgcccc
     2221 gatgtcttcc tgcaccactt cgtccacatc ccctggaccc agcccgacgc ctggcgcgtc
     2281 ctgccgcgcg acatgcgcga ggacgtcttc gagggcatcc tcgccaacga catcgtcgcc
     2341 ttccacaccc gcgcctaccg gcgcaacttc ctgctctgct gccgggagct gttcgacctc
     2401 gacgtcgacg agcgcgccgg cgtagtgcgc gtcgacggtc gcgacgtctg ggtccgcgcc
     2461 tatccgctgc cgatctccgc cgacaccttc cggcgcaccg cccagcggcc tgccgtgcac
     2521 gagtacgagc gcgagatcct gcgccgccgg cgcgagcacc tgatcctgcg cgtcgaccgc
     2581 gccgacctgt cgaagaacat cctgcgcggg ttctcggcct tcgacctctt cctcgagcag
     2641 caccccgagt tcaaggagga ggtcacgttc atcgcgcacc tgatcccgtc gcgtcaggac
     2701 gtgccccagt acgccgagta cctcgagcgg atcgaggcgc tggtcgagga agaggtcgaa
     2761 ggccgagaac ccgcgcagga tgttcttcga caggtcggcg cggtcgacgc gcaggatcag
     2821 gtgctcgcgc cggcggcgca ggatct
//
LOCUS       DATABV010000049         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2024684, whole genome shotgun sequence.
ACCESSION   DATABV010000049 DATABV010000000
VERSION     DATABV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024684"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /gene="pyk"
                     /locus_tag="VHF88_02025"
     CDS             complement(<1..1242)
                     /gene="pyk"
                     /locus_tag="VHF88_02025"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX2160578.1"
                     /translation="MRRTKIVATIGPASADPETLARLVEAGLDVARLNFAHGTPESQA
                     ATAERVRAAADRAGRQVAILQDLPGPKLRIGPLRDDVAELHAGETLTLLCGETLEGDA
                     TRMSVAWAGLCGAVEPGDEIFLADGSVRLRVERVREGEGEVDACVDLGGVVASRQGVN
                     VPGELMALPSVPEGDLELLRCGESIGVDMVALSFVRRPEDVEDVRRHTELPLIAKIEK
                     PQAVRNAEAIVTAADCVMVARGDLGIELELEQVPLVQKRIIALAGEHARPVITATQML
                     ESMVTSSRPTRAEVTDVANAILDGTDALMLSQETAVGAYPVEAVRMMASIAERTEPAA
                     PMVEWNEHRVARDARDPAYTVAYNACAAARQLGLSAILCPTLSGRSARLISAHRPEVP
                     VYALSPSDEVVRRCSLMWGVEA"
     gene            complement(1239..2270)
                     /locus_tag="VHF88_02030"
     CDS             complement(1239..2270)
                     /locus_tag="VHF88_02030"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX2160579.1"
                     /translation="MRVGMLTGGGDCPGLNAVIRGIVRRGAQLGDHDFVGFRNGWAGA
                     IAGSVTELTPQTTAGILPRGGTILGSSRTNPYKEDGGPERIVATLEAHGVDALVPIGG
                     EDTLGVARRLHEDHGIPIVGVPKTIDNDLGGTDFTFGFQTAVQIATDAIDRLHTTAES
                     HNRVMILEVMGRHAGWIAAYSGMAGGADAILVPERPFDIDAVSASIERRHAGGRTFSI
                     VVVSEGAMPAGGMEVGDGKQTDAFGHVRLGGIAVQLEREIEQRTGFESRMTILGHIQR
                     GGTPIAYDRVLATRFGVAAMDAVADGAFGQMVGLHATDMVRVGLDEALREPKLLDPSL
                     YETAEVFFG"
     gene            complement(2274..2480)
                     /locus_tag="VHF88_02035"
     CDS             complement(2274..2480)
                     /locus_tag="VHF88_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160580.1"
                     /translation="MSGSGAPKRDTPDNEDFEGAPQESGQQPEEAPPEVAGQGGRERK
                     RGGEGHGTGERDDDRRGDEGEGQG"
     gene            complement(2477..3202)
                     /locus_tag="VHF88_02040"
     CDS             complement(2477..3202)
                     /locus_tag="VHF88_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2160581.1"
                     /translation="MTPTIGICPAVERARWSHWEDEVVLTPRTYVDAVQLTGGLALVL
                     PPDDEVAEKPDELLDKLDGLLLTGGADVDPASYGARPDPRTGPTRPERDRFELALAYG
                     AMERKLPLLGICRGGQMVNVACGGTLIQHLPDVVGDDRHLETPGTFSEHEVELEHGSL
                     AARAAGADRIAVRSHHHQAVDELGEGLKATGHALPDGTVEAVEHPGHDFTLGVLWHPE
                     ADERSKIVAALVRAAKPRVASNR"
     gene            3251..3487
                     /locus_tag="VHF88_02045"
     CDS             3251..3487
                     /locus_tag="VHF88_02045"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160582.1"
                     /translation="MDLRKEKVVVETDRYRVTGSMTLPADGFRSRLSDHVNREDLTFF
                     TIEDAELVPLDGGESRTVRVLMLARAHVRLVIPG"
     gene            complement(3511..3597)
                     /locus_tag="VHF88_02050"
     tRNA            complement(3511..3597)
                     /locus_tag="VHF88_02050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3561..3563),aa:Ser,seq:gga)
BASE COUNT          464 a         1362 c         1309 g          529 t
ORIGIN      
        1 cgcctcgacg ccccacatca ggctgcagcg gcggaccacc tcgtcgctcg gcgacagcgc
       61 gtagaccggc acctccgggc ggtgcgcgga tatcaagcgc gccgagcgcc cggacagcgt
      121 cgggcagagg atcgcgctga ggccgagctg tcgcgccgcg gcgcaggcgt tgtaggcgac
      181 ggtgtaggcg ggatcgcgcg cgtcgcgggc gacgcggtgc tcgttccact cgaccatcgg
      241 cgccgccggc tcggtccgct ccgcgatcga cgccatcatc cgcaccgcct cgacggggta
      301 ggcgccgaca gcggtctcct gcgagagcat cagcgcgtcg gtgccgtcga ggatcgcgtt
      361 cgcgacgtcg gtcacctccg cgcgcgtcgg acgcgacgag gtgaccatcg actcgagcat
      421 ctgggtcgcg gtgatcaccg gccgcgcgtg ctcgccggcg agcgcgatga tccgcttctg
      481 gaccagcggc acctgctcga gctcgagctc gatgccgagg tcgccgcggg cgaccatcac
      541 gcagtcggct gcggtgacga tcgcctcggc gttgcgcacc gcctgcggct tctcgatctt
      601 cgcgatcagc ggcagctcag tgtgcctgcg cacgtcctcg acgtcctcgg ggcggcggac
      661 gaacgacagc gcgaccatgt cgacgccgat cgactcgccg cagcgcagca gctccagatc
      721 gccctcgggc accgacggca gcgccatcag ctcgccgggc acgttgacgc cctggcgcga
      781 ggcgacgact ccgccgaggt cgacgcaggc gtcgacctcg ccctccccct cgcgcacgcg
      841 ctcgacgcgc aggcgcaccg acccgtcggc gaggaagatc tcgtcgccgg gctcgaccgc
      901 gccgcagagc ccggcccagg cgaccgacat ccgcgtcgcg tcgccctcga gcgtctcgcc
      961 gcagaggagc gtcagcgtct cgccggcgtg cagctcggcg acgtcgtcgc gcagcgggcc
     1021 gatgcgcagc ttcgggccgg ggaggtcctg gaggatcgcc acctggcggc cggcgcggtc
     1081 ggcggcggca cgcacccgct cggccgtcgc agcctggctc tccggcgtgc cgtgcgcgaa
     1141 gttcaggcgc gcgacgtcga ggccggcctc gaccagccgc gcgagcgtct cggggtcggc
     1201 ggacgccggc ccgatcgttg cgacgatctt cgtccgcctc acccgaagaa gacctcggcc
     1261 gtctcgtaga gcgacgggtc gagcagcttc ggctcgcgca gcgcctcgtc gagcccgacg
     1321 cggaccatgt cggtcgcgtg caggcccacc atctgcccga acgcgccgtc ggcgacggcg
     1381 tccatcgcgg cgacgccgaa gcgggttgcg agcacgcggt cgtaggcgat cggggtgccg
     1441 ccgcgctgga tgtgaccgag gatcgtcatc cggctctcga agccggtccg ctgctcgatc
     1501 tcgcgctcga gctggacggc gatcccgccg agccggacgt ggccgaaggc gtcggtctgc
     1561 ttgccgtctc cgacctccat cccgccggcc ggcatcgcgc cctccgatac gacgacgatc
     1621 gagaacgtgc ggccgccggc gtgacggcgc tcgatgctgg cgctgaccgc gtcgatgtcg
     1681 aacggtcgct cgggcacgag gatcgcgtcg gcgccgccgg ccatgccgct gtaagcggcg
     1741 atccagcccg cgtgacggcc catgacctcg aggatcatca cgcggttgtg cgactcggcg
     1801 gtcgtgtgca ggcggtcgat cgcgtccgtt gcgatctgga ccgcggtctg gaagccgaag
     1861 gtgaagtcgg tgccgccgag gtcgttgtcg atcgtcttcg gcacgccgac gatcgggatg
     1921 ccgtggtcct cgtgcagccg ccgcgccacg ccgagcgtgt cctcgccgcc gatcgggacg
     1981 agcgcgtcga cgccatgggc ctcgagcgtc gcgacgatcc tctccggccc gccgtcctcc
     2041 ttgtaggggt tcgtgcgcga gctgccgagg atcgtgccgc cccgcggaag gatgcccgcg
     2101 gtggtctggg gcgtgagctc ggtgacgctg ccggcgatcg cgccggccca gccgttgcgg
     2161 aagccgacga agtcgtgatc gcccaactgc gcgccgcgcc ggacgatgcc gcggatcacc
     2221 gcgttcaggc ccgggcagtc accgcccccc gtgagcatgc cgacgcgcac gggctacccc
     2281 tggccctccc cctcgtcccc cctgcgatcg tcgtcgcgct cgcccgtgcc gtggccctcg
     2341 ccgccgcgct tgcgctcgcg gccgccttgc cccgccacct ccggcggcgc ctcctcgggc
     2401 tgctgaccag actcctgggg cgcgccctcg aagtcctcat tgtccggcgt gtcgcgcttc
     2461 ggcgcccccg atccgctcat cgattcgatg ctacccgcgg cttcgcggcg cgtaccaagg
     2521 cggcgacgat cttgctgcgc tcgtcggcct ccgggtgcca gaggacgccg agcgtgaagt
     2581 cgtggccggg gtgctcgacg gcctcgacag tcccgtccgg aagggcgtgg ccggtcgcct
     2641 tcagcccctc gccgagctcg tcgaccgcct ggtggtggtg ggagcggacg gcgatccggt
     2701 cggctccggc ggcgcgcgcg gcgagcgagc cgtgctcgag ctcgacctcg tgctcggaga
     2761 aggtgccggg agtctcgagg tggcgatcgt cgccgacgac gtcgggaagg tgctggatca
     2821 gcgtgccgcc gcaggcgacg ttgaccatct ggccgccgcg gcagatgccg agcagcggaa
     2881 gcttgcgctc catcgccccg taggcgagcg ccagctcgaa gcggtcgcgc tcggggcggg
     2941 tcggaccggt gcgggggtcg ggccgcgcgc cgtagctcgc ggggtcgacg tccgcgccgc
     3001 cggtcagcag cagcccgtcg agcttgtcga gcagctcgtc cggcttctcg gcgacctcgt
     3061 cgtcgggcgg caggaccagc gccaggccgc cggttagctg gaccgcgtcg acgtacgtgc
     3121 gcggcgtgag caccacctcg tcctcccagt ggctccagcg cgcgcgctcg accgcggggc
     3181 agattccgat cgtcggcgtc atgggtcgaa ccgtactcgg gcgcgccgcg ctgccatagg
     3241 atcgccggcc atggacctgc gcaaggagaa ggtcgtcgtc gagaccgacc gctaccgcgt
     3301 caccggctcg atgaccctgc ccgccgacgg cttccgcagc cgcctctccg accacgtcaa
     3361 ccgcgaggac ctcaccttct tcacgatcga ggacgccgag ctcgtcccgc tcgacggcgg
     3421 agagtcacgc acggtccggg tgctcatgct cgcccgggct cacgtgcgac tcgtgatccc
     3481 gggctgaccg aagctcccgc ggccgggagg cggtgagggt gggattcgaa cccacgatac
     3541 ggtttcccgc atacacgctt tccaagcgtg ctccttaagc cactcggaca cctcaccggg
     3601 gtccggcaag cctacaagcc caggtcgcgc acgccgcgct cgtcccagcc gaggtggtgg
     3661 gcga
//
LOCUS       DATABV010000050         8758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1461454, whole genome shotgun sequence.
ACCESSION   DATABV010000050 DATABV010000000
VERSION     DATABV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461454"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1564
                     /locus_tag="VHF88_02055"
     CDS             107..1564
                     /locus_tag="VHF88_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2160583.1"
                     /translation="MTATTDRPRDQRRIPRRPQLGGRSLVPAFGGARARILASFLILL
                     AFSTAATVIVIRQILLVRLEAEVSDTLVQETEEFSQLVQGRDPATGEPFGDDLRRIFD
                     VYIDRNVPDDDEAIFTILNGQPYASIGSRQFPIEALAQREHWTALRRPTQKDVETSAG
                     PARYLAVPIRLGGEIRGHFVVAEFMRGDRAEVADAVRIAALVSGSVLLIASLLAFFAA
                     GRVLAPLRALGDTARSIHETDLTRRIDVEGNDELADLARTFNAMLDRVETAFETQRDF
                     IRDASHELRTPITIVSGHLELMGDDPEERRATVDLVMDELARMSRLVDDLLLLARAER
                     SDFLQVGPVDLEELAEELLAKASALAPRDWRLEAHARVTIEADRQRLTQAVMALADNA
                     AQHTTDDDSITLGTAVEGSRIRLWVSDTGPGVSADDQRRIFDRFARGTSGPRRSDGSG
                     LGLAIVKAIAEAHGGTVHVESRPGAGATFSLRIPT"
     gene            1561..2229
                     /locus_tag="VHF88_02060"
     CDS             1561..2229
                     /locus_tag="VHF88_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2160584.1"
                     /translation="MSRILIAEDEPRLASFLEKGLKANGYVTTVVADGHVASIVARDD
                     EFDLMILDIGLPRMDGFHVLKSIRERGQRLPVIVLTARDDVEDKVRGLEGGADDYVTK
                     PFRFEELLARVRVRLRTDGRDAETVLEVGPISLDLRSRKARAGDAEVTLTAREFTLLE
                     TFMRHPGQMLSREQLLSHVWGYDYDPGSNIVDVYIGYLRRKLGDDSIETVRGMGYRLR
                     AEAS"
     gene            complement(2202..3173)
                     /locus_tag="VHF88_02065"
     CDS             complement(2202..3173)
                     /locus_tag="VHF88_02065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2160585.1"
                     /translation="MTAAPPLPRGAAIAPGYEVIEHLRRGNALDVYDVWSEQRACRCI
                     VKALRPDRGDDAGARRRLIEEGGLLAALSHPHIVRGYETIAEPLPLVVMETLVGETLG
                     HMVRERQVELTVEEIAQLGLHLASAVRYLHLNGYLHLDLKPSNVIAECGRAKLIDLSV
                     ARPPGRAHAGIGTWCYMAPEQVRGGELGPAADVWGIGAVLLEAATGEPAFDEPEASSR
                     SGYAEDDASGWVSTDGGSSSASSGELESLDAADHPQIRARAPRLDERRPLSAALSDLV
                     AACLEPEPGDRPSVRDLLAALEPLAGVPAAERRHAAIAGQDASARRR"
     gene            complement(3170..5023)
                     /locus_tag="VHF88_02070"
     CDS             complement(3170..5023)
                     /locus_tag="VHF88_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2160586.1"
                     /translation="MRALRERIRLLFVPSGEGALVAAAPPVAVREIFRRFWPDARPYR
                     RWIPVGLLFIAAGAALETAEIWVFKLVVDDVLVPGDLGPLLGIGLLYLGLTIANALVS
                     FGDDYLATWLGERFLQRLRRRVFSHVQRLSLDVLDRRRIGDVTARLTSDVQAIESFVL
                     SGVADGLSAILRIVFFSGALFYLQWKLALVALIVTPLFLFVARRFARLIKHASREKRR
                     RSGSLSAVAQESLGNHALVQASNREQAELERFERENEGIIQAELASTRIRGLFSPVVD
                     LIELAGVMLVLLLGTVAVADGSLTLGGLLVFIAYLTQLYGPVRDLSSLSNTIFKAAAG
                     AERVIELLDEQPTIVDKPNAVRLERARGVVELDAVSFGYPGTERVALADISLRAEPGE
                     TVALVGASGAGKSTIAKLLLRFYDPGAGAVRLDGHDLRDLELASLRRNVAILLQETLV
                     LHGTVRENIAYSRPDASDGDVRRAAAAAGADEFVQALPDGYETSLGERGRRLSGGQRQ
                     RIAIARALLADAPVLVLDEPSTGLDAEARAALVAPLRALMAERTTIVISHDLLTVRDA
                     DRIVVLDDGRIVEQGGHDELVAAGGHYARLWALHGVDHPGVPIGPGGSIAG"
     gene            5368..6888
                     /locus_tag="VHF88_02075"
     CDS             5368..6888
                     /locus_tag="VHF88_02075"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2160587.1"
                     /translation="MTDLAAALERARAGGPERHREKAAQQGKLPVRERIALLVDDGSF
                     VEDAALAAWEQDGFGADGVVTGMGTVDGRPVSLMANDPTVKAGSWGPKTVEKIIRAQE
                     AALEHRVPMIYLVDSAGARITEQVAMFPGRRHAGRIFSTEVRMSGVVPQVCVLFGPSA
                     AGGAYIPAFCDVVIMRDGNASMYLGSPRMAEMVIGEKVTLEEMGGARMHTGVSGCGHF
                     LVKTDAEGIALARRYLSYFPTNWEGTPPLAPPAEPASDAPIRDIVPADENKPFDMREL
                     LDSLVDAGSFLEVHKRWAKELIVGYARLDGQVVGVVANQPKQKGGVLFVDSSDKAARF
                     IWTCNAFNVPLLFLADVPGFMIGTQVERQGMIRAGAKMISAVTEATVPKISVVVRKAY
                     GAGLYAMAGPAFDPDACIALPSAKIAVMGPQAAINAVFYNQVQAIEDERERAALVEKL
                     RAEYAEEIDTLHLASELVVDAVIQPEDLRAELIQRYRLYAGRVREWPAKHNAVTPV"
     gene            complement(6912..6995)
                     /locus_tag="VHF88_02080"
     tRNA            complement(6912..6995)
                     /locus_tag="VHF88_02080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6959..6961),aa:Leu,seq:caa)
     gene            complement(7015..7515)
                     /locus_tag="VHF88_02085"
     CDS             complement(7015..7515)
                     /locus_tag="VHF88_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160588.1"
                     /translation="MAEPLIHHPNREKADSKAMKSIVVLLLIVSAAMVLLITIGGWSA
                     LQGGQPMAFVYAFLNLLFAYYVSRWNRGVLPVAAALAVLFGVLALVAGPSWFSRDKAG
                     FDDPALDPAMLGLLTFALVPIQALLVAFCARAFSQRWNVEVEVDPDDPYYDAPAHRGG
                     AQPLGA"
     gene            complement(7575..7656)
                     /locus_tag="VHF88_02090"
     tRNA            complement(7575..7656)
                     /locus_tag="VHF88_02090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7620..7622),aa:Leu,seq:tag)
     gene            7744..>8758
                     /locus_tag="VHF88_02095"
     CDS             7744..>8758
                     /locus_tag="VHF88_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX2160589.1"
                     /translation="MDADVVKPTWNDGKRYAWLLGLLVPSLPLMAWGLVETTGMGVFW
                     FVGPFVVFGLFPVLDLLIGTDQENPPDSVVAWLEQDPYYRWVTYLFIPLQYLSLVLAC
                     WLWSSGGLSTLEGVGLALTTAVVAGVAINTAHELGHKRASLERWLSKVALAQTGYGHF
                     FIEHNRGHHVRVATPEDPASSRLGESFWEFLPRTVVGSLTSSWELEAKRLRRMGKGVW
                     SVHNDILNAWAMTVVLFGGLVAVFGVVVLPYLLVQAVLGFSLLEVVNYLEHYGLKRGR
                     RPDGRYEITRPEHSWNSNNVASNVLLYHLQRHSDHHANPLRRYQALRHEDEAPQLPTG
                     YAGM"
BASE COUNT         1334 a         3091 c         3097 g         1236 t
ORIGIN      
        1 ccggctccag ccccggtcgc cccgccaccg ccgcagcccg tgcccgatga cgacggcgac
       61 gacgacgagg gtgacgacga cagcggcgat gatggggccg gggacgatga cggcgacgac
      121 tgacaggcca cgcgatcagc gccggattcc ccgccggccc cagctgggcg gacggagcct
      181 cgtccccgcc ttcgggggtg cccgcgcgcg catcctcgcc tcgttcctga tcctgctcgc
      241 gttctcgacc gccgcgacgg tgatcgtgat ccgccagatc ctgctcgtgc ggctcgaggc
      301 cgaggtcagc gacacgctcg tgcaggagac ggaggagttc agccagctcg tgcagggtcg
      361 cgatccggcg accggcgagc cgttcgggga cgacctgcgg cggatcttcg acgtctacat
      421 cgaccgcaac gtccccgacg acgacgaggc gatcttcacg atcctcaacg gacagccgta
      481 cgcgtcgatc ggcagccgcc agttcccgat cgaggcgctg gcgcagcgcg agcactggac
      541 agcgctgcgc aggccgacgc agaaggacgt cgagacgtcg gccgggccgg cacgctacct
      601 ggccgtcccg attcggctcg gcggcgagat ccgcggtcac ttcgtcgtgg ccgagttcat
      661 gcgcggcgac cgcgccgagg tcgccgacgc ggtgcgcatc gccgcgctcg tcagcggatc
      721 ggtcctgctg atcgcgtcgt tgctcgcctt cttcgcggcc ggccgcgtgc tcgcgccgct
      781 cagggcgctc ggcgacacgg cgcgctcgat ccacgagacc gacctcacgc gccgcatcga
      841 tgtcgagggc aacgacgagc tcgccgacct cgcgcgaacc ttcaacgcga tgctggaccg
      901 tgtggagacg gcgttcgaga cgcagcgcga cttcatccgc gacgcgagcc acgagctccg
      961 caccccgatc acgatcgtca gcggccacct cgagctgatg ggcgacgacc ccgaagagcg
     1021 gcgggcgacg gtcgacctcg tgatggatga gctcgcccgg atgagccggc tggtcgacga
     1081 cctgctgctg ctggcgcgcg ccgagcgcag cgacttcctg caggtggggc cggtcgacct
     1141 cgaagagctg gcggaggagc tgctcgccaa ggcaagcgcg ctcgccccgc gcgactggcg
     1201 cctagaagcc cacgcccggg tgacgatcga ggccgaccgc cagcgcctga cccaggccgt
     1261 gatggcgctt gccgacaacg ccgcccagca caccaccgac gacgactcga tcacgcttgg
     1321 gaccgccgtc gagggctcgc ggattcgcct ctgggtgtcg gacaccgggc cgggcgtctc
     1381 cgcagacgac cagcggcgga tcttcgaccg tttcgcgcgc ggcacctccg gcccgcggcg
     1441 ctccgacggc tcggggctcg ggctcgcgat cgtcaaggcg attgccgagg ctcacggagg
     1501 caccgtccac gtcgagagcc ggcccggcgc cggagcgact ttcagcctgc ggataccgac
     1561 atgagccgga tcctgatcgc cgaggacgag ccgcggctcg cttcattcct cgagaagggc
     1621 ctcaaggcga acggctacgt aaccacggtc gtcgccgacg gccacgtcgc cagcatcgtc
     1681 gcccgcgacg acgagttcga cctgatgatc ctcgacatcg gcctgccgcg catggatggc
     1741 ttccacgtgc tgaagtcgat ccgcgagcgt ggccagcggc tgccggtgat cgtgctcacg
     1801 gctcgcgacg acgtcgagga caaggtgcgg gggctcgagg gcggcgccga cgactacgtg
     1861 accaagccct tccgcttcga ggagctgctg gcgcgcgtgc gcgtgcggct gcgcaccgac
     1921 gggcgcgacg ccgagacggt gctcgaggtc gggccgatct cgctcgacct gcgcagccgc
     1981 aaggcccgcg ccggcgacgc cgaggtcacg ctgacggcgc gcgagttcac cctcctcgag
     2041 acgttcatgc gccatcccgg ccagatgctc tcgcgcgagc agctgctctc ccacgtctgg
     2101 ggctacgact acgaccccgg ctcgaacatc gtcgacgtct acatcggcta tctgcgtcgc
     2161 aagctcggcg acgattcgat cgagacggtg cgcggtatgg gctaccgcct gcgcgccgaa
     2221 gcgtcctgac cggcgatggc ggcatggcgg cgttcggcgg ccgggacgcc ggccagcggc
     2281 tcgagggcgg cgagcaggtc gcggaccgac ggccggtcgc ccggctcggg ctcgaggcac
     2341 gcggccacca ggtccgacag ggccgcggac agcggccgac gctcgtcgag ccggggggcc
     2401 cgcgcgcgga tctgcggatg gtcggccgcg tcgagcgact ccagctcgcc cgaggacgcg
     2461 gagctgctgc cgccgtcggt cgagacccag ccgctcgcgt cgtcctccgc gtagccgctg
     2521 cgcgacgatg cctccggctc gtcgaaggcc ggctcgccgg tcgctgcctc gagcagcacc
     2581 gcgccgatcc cccagacgtc ggcggccggg ccgagctcgc cgccgcgcac ctgctccggt
     2641 gccatgtagc accacgtccc gatcccggcg tgcgctcggc cgggaggacg cgccacgctg
     2701 agatcgatca gcttcgcgcg tccgcactcg gcgatcacgt tcgacggctt gaggtcgagg
     2761 tgcagatagc cgttgaggtg cagatagcgg acggccgacg ccagatgcag cccgagctga
     2821 gcgatctcct cgaccgtcaa ctcgacctgc cgctcgcgga ccatgtggcc gagcgtctcg
     2881 ccgaccaagg tctccatcac aaccagcggc agcggctcgg cgatcgtctc gtagccgcgg
     2941 acgatgtgtg gatgcgagag cgcggcgagc agtccgccct cctcgatcag ccggcggcgg
     3001 gcgccggcgt cgtcgccgcg gtccggtcgt agcgccttga cgatgcagcg gcacgcgcgc
     3061 tgctcgctcc agacgtcgta gacgtcgagc gcgttgccgc ggcgcaggtg ctcgatgacc
     3121 tcgtagccgg gcgcgatcgc ggcgccgcgg ggcagcggag gagcggccgt catcccgcga
     3181 tgctcccacc agggccgatt gggacgccag gatggtcgac gccgtgcagc gcccacagcc
     3241 gggcgtagtg cccgcccgcg gcgaccagct cgtcgtggcc gccctgctcc acgatccggc
     3301 cgtcgtcgag cacgacgatc cggtcggcgt cgcgcacggt cagcaggtcg tgcgagatca
     3361 cgatcgtcgt gcgctcggcc atcagcgcgc gcagcggcgc gacgagcgcc gcgcgcgcct
     3421 cggcgtccaa tcccgtcgac ggctcgtcga gcaccagcac cggcgcgtcc gcgagcagcg
     3481 cccgcgcgat cgcgatccgt tgacgctgtc cgccggacag ccgacggccg cgctcgccga
     3541 ggctggtctc gtagccgtcg ggcagcgctt ggacgaactc gtcggcgccg gcggcggcgg
     3601 ccgcccggcg gacgtccccg tccgaggcgt ccggtcgcga gtaggcgatg ttctcgcgaa
     3661 ccgtgccgtg caggacgagc gtctcctgca gcaggatcgc gacgttgcgg cgcagcgacg
     3721 ccagctcgag gtcgcgcagg tcgtggccgt ccaaccgaac cgctccggcg ccggggtcgt
     3781 agaagcgcag cagcagcttg gcgatcgtcg acttgccggc gcccgacgcg ccgaccagcg
     3841 cgaccgtctc gcccggctcc gcgcgcaggc tgatgtcggc gagcgcgacg cgctcggtgc
     3901 cgggatagcc gaacgacacg gcgtcgagct cgaccacgcc acgtgctcgc tcgaggcgga
     3961 cggcgttcgg cttgtcgacg atcgtcggct gctcgtcgag cagctcgatc acgcgctcgg
     4021 ctccggcggc cgccttgaag atcgtgttcg acagcgaact gaggtcgcgc acgggcccgt
     4081 agagctgggt caggtaggcg atgaagacga gcaggccgcc gagcgtcagg cttccgtccg
     4141 cgaccgccac cgtcccgagc agcagcacga gcatcacacc ggcgagctcg atcaggtcga
     4201 ccacggggga gaagaggccg cggatccgcg tcgacgcgag ctcggcctgg atgatgccct
     4261 cgttctcgcg ctcgaagcgc tcgagctcgg cctgctcgcg gttcgaggcc tggacgagcg
     4321 cgtggttgcc gaggctctcc tgggccacgg cgctgagcga gcccgagcgg cgccgcttct
     4381 cgcgcgaggc gtgcttgatc agccgcgcga agcgccgggc gacgaacagg aacagcggcg
     4441 tgacgatcag tgccacgagc gccagcttcc attgcaggta gaacagcgct ccgctgaaga
     4501 agacgatccg caggatcgcc gagaggccgt cggctacgcc cgagagcacg aagctctcga
     4561 tcgcctggac gtcgctggtc agccgcgcgg tgacgtcgcc gatccgccgg cggtcgagca
     4621 cgtccagcga cagccgctgg acgtgggaga agacgcgccg ccgcagccgc tggaggaagc
     4681 gctccccaag ccacgtcgcg aggtagtcgt cgccgaacga gacgagcgcg ttggcgatcg
     4741 tgagcccgag gtagagcagc ccgatcccga gcaggggacc gaggtcgccc gggacgagca
     4801 cgtcgtcgac gacgagcttg aagacccaga tctcggcggt ctcgagcgcg gcgccggccg
     4861 cgatgaacag caggccgacc gggatccagc ggcgataggg ccgggcgtcg ggccagaagc
     4921 ggcggaagat ctcccgcacc gccaccggcg gcgctgcagc gacgagcgcg ccctcacccg
     4981 agggcacgaa cagcaaacgg atccgttcgc gtagggcgcg catggcgtgc agccagcctg
     5041 acacgcgaag acggccccgc cgggcggggc cgtctccaag cgttcgctga gtcgcctgag
     5101 gtggctagct gttggtaccg cgacgacggc ggcgcatacg cctgcgacgg cgcctgcgac
     5161 gcctccgccc gctgttggtg ttgctgttgg tgccgccgct gttgctcgcg gcctgggcgc
     5221 cgactcgtgc tgctggatag gtattcctga atgccactgg tcacacctcc tggggttagg
     5281 actgcaatca atctgccccg ggtcggtgag gccagcgtga ggcgtggatt agagcctttt
     5341 cacgtactcc cgtgagagac tgcgcccgtg accgacctag ccgcagctct cgagcgcgcc
     5401 cgcgccggcg ggcccgagcg ccaccgcgag aaggcggctc agcagggcaa gctgcccgtc
     5461 cgcgaacgca tcgctttgct ggtcgacgat ggctcgttcg tcgaggacgc ggcgctcgcg
     5521 gcttgggagc aggacggctt cggcgccgac ggcgtcgtca ccgggatggg gaccgtcgat
     5581 ggtcggcccg tatcgctgat ggccaacgac ccgacggtca aagcggggtc gtgggggccc
     5641 aagaccgtcg agaagatcat tcgcgcgcag gaggccgcac tcgagcaccg cgtgccgatg
     5701 atctacctcg tcgactcggc cggcgcgcgg atcaccgagc aggtcgcgat gtttcccggc
     5761 cgccgccacg ccggtcggat cttctcgacc gaggtgcgga tgtccggcgt cgtcccgcag
     5821 gtctgcgtgc tgttcggtcc gagcgcagcc ggcggggcct acatcccggc cttctgcgac
     5881 gtcgtgatca tgcgcgacgg caacgcctcg atgtacctcg gctcgccgcg gatggccgag
     5941 atggtgatcg gcgagaaggt gacgctcgag gagatgggcg gggcgcggat gcacaccggc
     6001 gtctcgggct gcggccactt cctggtcaag accgacgcgg aggggatcgc gctcgcgcgc
     6061 cgttacctct cctacttccc gaccaactgg gaggggacgc cgccgctggc gccgcccgcc
     6121 gagccggcga gcgacgcgcc gatccgcgac atcgtcccgg ccgacgagaa caagccgttc
     6181 gacatgcgcg agctgctcga ctcactcgtc gacgccggct cgttcctcga ggtccacaag
     6241 cgctgggcga aggagctgat cgtgggctac gcccggctcg acggccaggt cgtcggggtc
     6301 gtcgccaacc agccgaagca gaagggggga gtgctgttcg tcgactcctc cgacaaggcg
     6361 gcgcgcttca tctggacctg caacgcgttc aacgtgccgc tgctcttcct cgccgacgtg
     6421 ccggggttca tgatcggcac ccaggtcgag cgccagggga tgatccgcgc cggcgcgaag
     6481 atgatctcgg ccgtgaccga ggcgaccgtg ccgaagatct cggtcgtcgt gcgcaaggcc
     6541 tacggcgccg gcctctacgc gatggccggg cccgcattcg accccgacgc ctgcatcgcg
     6601 ctgccgagcg cgaagatcgc ggtgatggga ccccaggcgg cgatcaacgc cgtcttctac
     6661 aaccaggtcc aggcgatcga ggatgagcgg gagcgggcgg cgctggtcga gaagttgcgc
     6721 gccgagtacg ccgaggagat cgacacgctg cacctcgcgt cggagctggt cgtcgacgcg
     6781 gtgatccagc ccgaggacct gcgcgccgag ttgatccagc gctaccggct ctacgccggc
     6841 cgcgtgcgcg agtggccggc caagcacaac gccgtcacgc ctgtctgacg cctgccggcc
     6901 gagcggccga gtgcggcggg tgggactcga acccacaagc ccttgcgagc agcggctttt
     6961 gagaccgcct cctttaccaa tttggacacc gccgcatgcg ggtcgagggc gattctaggc
     7021 gccgagcggc tgcgcgccgc cgcggtgtgc gggagcgtcg tagtacgggt cgtcggggtc
     7081 gacctcgacc tcgacgttcc agcgctggct gaaggcgcgc gcgcagaagg cgacgagcag
     7141 ggcctggatc ggcaccagcg cgaaggtcag caggccgagc atcgccggat cgagcgccgg
     7201 atcgtcgaag ccggccttgt cgcgggagaa ccacgacggt ccggcgacca gtgcgagcac
     7261 gccgaagagc accgcgagcg ccgccgcgac cggcagcacg ccgcggttcc agcgcgacac
     7321 gtagtaggcg aacagcaggt tgaggaacgc gtagacgaag gccattggct gtccgccctg
     7381 cagcgccgac cagccgccga tcgtgatcag cagcaccatc gccgcgctga cgatcagcag
     7441 cagcacgacg atcgacttca tcgccttgga gtccgccttc tcgcggttcg ggtggtggat
     7501 cagcggctca gccatcgctc ctcggcgctg agcctaccgc ctgccccgcg gcgaggcgac
     7561 ggacaaccga cccatgcgga tggagggact cgaaccccca cggccaaggc caccggcacc
     7621 taaagccggc gcgtctacca attccgccac atccgcgggc gagcgtcgat tgaagcagag
     7681 ccgtcgaggg ccggaccaaa tgcttgctag acaagatagg cagttcgtgt aaaactgctt
     7741 tacatggatg ccgacgttgt aaagcccact tggaatgacg gcaagcgcta cgcctggctg
     7801 ctcgggctgt tggtcccgag cctgccgttg atggcgtggg gcctggtcga gacgaccggc
     7861 atgggggtct tctggttcgt cgggccgttc gtcgtcttcg gcctgtttcc ggtgctcgac
     7921 ctgctgatcg gcaccgacca ggagaacccg cccgacagcg tcgtcgcctg gctcgagcag
     7981 gatccctact accgctgggt cacctacctg ttcatcccgc tgcagtacct ctcgctggtg
     8041 ctcgcgtgct ggctctggtc cagtggcggc ctctcgacgc tcgagggggt cggcctcgcg
     8101 ctgacgaccg ctgtggtcgc cggcgtcgcg atcaacaccg cccacgagct cggccacaag
     8161 cgcgcgagcc tcgagcgctg gctgagcaag gtcgcgctcg cgcagaccgg ctacggccac
     8221 ttcttcatcg agcacaaccg cggacaccac gtgcgtgtcg caacgccgga ggacccggcg
     8281 agctcgcggc tcggcgagag cttctgggag ttcctgccgc gcaccgtggt cggcagcctg
     8341 acctcgtcgt gggagctcga ggccaagcgg ctgcggcgga tgggcaaggg cgtctggtcg
     8401 gtccacaacg acatcctcaa cgcctgggcg atgaccgtcg ttctgttcgg cgggctggtc
     8461 gcggtcttcg gcgtggtcgt gctgccgtac ctgctcgtcc aggcggtgct cgggttctcg
     8521 ctgctggagg tcgtcaacta cctcgagcac tacgggctca agcgcggccg gcggcccgac
     8581 ggtcgctacg agatcacccg tcccgagcac agctggaaca gcaacaacgt cgcgtccaac
     8641 gtgctgctct accacctcca gcgccacagc gaccaccacg ccaacccgct gcgccgctac
     8701 caggcgctcc ggcacgagga cgaggcaccg cagctgccga cgggctacgc cgggatga
//
LOCUS       DATABV010000051         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2426125, whole genome shotgun sequence.
ACCESSION   DATABV010000051 DATABV010000000
VERSION     DATABV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426125"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VHF88_02100"
     CDS             <1..423
                     /locus_tag="VHF88_02100"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160590.1"
                     /translation="VRERAVADSMYDYVEHYGQNFDRALIAQPYLEGASDVRVLAIDG
                     EAAAAMERIPTGDWRANVELGAAAEPIAVDATIAGVTAEVGQRIGARIFGLDLFRHDG
                     ALIVGEVNHSPLFKGIVDATGIDVAGRIADHALGVAVR"
     gene            420..1583
                     /locus_tag="VHF88_02105"
     CDS             420..1583
                     /locus_tag="VHF88_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2160591.1"
                     /translation="MRAELAETEEATDATGAPRRAGVVGASGILGGELLRILVNHPGL
                     GLEYVSAQSRAGRSATELNPHLKDAGIGEFEAFDARVAAKRCDVVFLATPPETAGRAV
                     PELLDAGAELVVDLSPAYRVRDPELHRQWYPDVERDQGIAEAAAYALPELSRAAAGDG
                     GGGGSGLAPIGRDAIRGASVLATPGCFATAILLAALPLRELDGVSFDSIAIDGKSGST
                     GSGARLRESGAHTWRSNVVAPYAPRGHRHVAEVGEALAGAGVSVGRLGMSTYGVDLVR
                     GLSVGLYSFFEGELDERTLRRAYHRTYRDERFVRVQAGRGSALPLPDPKAVIGSNYCD
                     VAGFVDEAAGRFVLIAAIDNMVKGGAGQAVQACNLRLGLPEELGLEAIPVFPV"
     gene            1583..2473
                     /locus_tag="VHF88_02110"
     CDS             1583..2473
                     /locus_tag="VHF88_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160592.1"
                     /translation="MPGRPPQPPGLLVVKVGGSCLEQLGPEWWDALTALVRGSDRPAT
                     ATSGGDGGDGHASRSRTKLVVVHGWSRMVSAATPEGQPDFIVDKHGFRSRYTTAEVLE
                     EIERVAGDLRERIAELLRKRGLRAAAVSGAETRLLHAEVRQNMWWVGDELVPLTNLVG
                     PVREVDRARLDVLLAESDAVVVSPLAVTAEHPRVNTDADRAAAAIAGAGDAERLALVM
                     DAEAVLADGEPVRAAARADLKELARHATGGMSKKLGAVEEAFAGGVREVAIGSATVPD
                     LLAGRAGTLITAEPDPSPAR"
     gene            2528..>3627
                     /locus_tag="VHF88_02115"
     CDS             2528..>3627
                     /locus_tag="VHF88_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2160593.1"
                     /translation="MAAELQQVPPAMAELRAEARAACPDGLLDLSRSEPLPIPLTDDC
                     AARVAEQFMRARQYSPSAGLTAVRDAAARYASVRTELELDAADVVITAGAMAGLAVAL
                     RALIEPGDEVLVPVPYFHSYPRQVSMLGGIPRLVDTRPRQGELTAQAIERELTPQTRA
                     LLLCNPANPAGVVLDRERLTAILAALPPSVAVIADEVYAEYVYGDAAPRELAGGDGER
                     RELVGPAGNSTTGPSGDRLVPRATDFVSALAVVADEDAGREVIALDTASKTAAMPGWR
                     VGTAIATAGLAPRLAQAAATLSGAPNTLAQLAFAAWLDDPPQLDRMAPYRPRLRDVLE
                     ELDDSGLTVRPPDGSYYVWLTAPAGDRPTAGRS"
BASE COUNT          466 a         1288 c         1414 g          459 t
ORIGIN      
        1 gtgcgcgagc gcgcggtcgc cgacagcatg tacgactacg tcgagcacta cggccagaac
       61 ttcgaccgcg cgctgatcgc ccagccgtac ctcgagggcg cgtccgacgt gcgcgtgctg
      121 gcgatcgacg gcgaggcggc cgcggcgatg gagcggatcc cgaccggcga ctggcgggcg
      181 aacgtggagc tcggcgccgc cgccgagccg atcgcggtcg acgcgacgat cgcgggagtg
      241 accgccgagg tcggccagcg gatcggggcg cggatcttcg ggttggacct gtttcggcac
      301 gacggcgcgc tgatcgtcgg cgaggtcaac cactcgccgc tgttcaaggg gatcgtcgac
      361 gcgacgggga tcgacgtggc ggggcggatc gccgaccacg cgctcggggt ggcggtgcgg
      421 tgagggcgga gctcgcagag acggaggagg cgacggacgc gacgggcgcg cccaggcgcg
      481 ccggcgtcgt cggcgcgagc ggcatcctcg gcggcgagct gctgcgcatc ctcgtcaacc
      541 acccggggct cgggctcgag tacgtcagcg cgcagagccg ggccgggcgg tcggccaccg
      601 agctcaaccc ccacctcaag gacgccggca tcggcgagtt cgaggccttc gacgcgcgcg
      661 tggcggcgaa gcgctgcgat gtcgtcttcc tcgcgacgcc gccggagacg gccgggcgcg
      721 cggtgcccga gctgctggac gcgggggccg agctggtcgt cgacctcagc ccggcgtacc
      781 gggtgcgcga cccggagctt caccggcagt ggtacccgga cgtggagcgg gatcagggga
      841 tcgcggaggc cgcggcgtac gcgctgccgg agctgagtcg cgcggcggcg ggcgacggcg
      901 gcggtggcgg gagcggcttg gcgccgatcg gacgcgacgc gatccgaggc gcgagcgttc
      961 ttgcgacacc cggctgcttc gcgaccgcga tcctgctcgc ggcgctaccg ctgcgcgagc
     1021 tcgacggcgt ctccttcgac tcgatcgcga tcgacggcaa gtcgggctcg accggctcgg
     1081 gcgcgcggct gcgcgagtcc ggcgcccaca cctggcgctc gaacgtcgtt gcgccatacg
     1141 cgccgcgcgg ccaccgtcac gtggccgagg tcggcgaggc gctcgcgggt gccggagtat
     1201 cggtcggccg gctcggcatg tcgacctatg gggtcgacct cgtgcgcggg ctgtcggtcg
     1261 ggctctactc gttcttcgag ggcgagctcg acgagcgcac gctacggcgg gcgtaccacc
     1321 gcacgtaccg cgacgagcgc ttcgtgcggg tccaggcggg gcgcggctcg gcgctgccgc
     1381 tgccggaccc gaaggcggtg atcgggtcga actactgcga cgtggcgggg ttcgtcgacg
     1441 aggccgcggg ccgcttcgtc ctgatcgcgg cgatcgacaa catggtcaag ggcggcgccg
     1501 gccaggcggt ccaggcctgc aacctgcggc tcgggctgcc cgaggagctc gggctcgagg
     1561 cgatccccgt cttccccgtg tgatgcccgg ccggccgccg cagccgcccg gtctgctggt
     1621 cgtgaaggtc ggcggcagct gcctagagca gctgggaccg gagtggtggg atgcgttgac
     1681 tgcgctagtt cgcggatccg accggcccgc gacggcgacg agcggcggcg atggcggtga
     1741 cgggcatgcg agccggtcgc ggaccaagct cgtcgtcgtc cacggctggt cgcggatggt
     1801 cagcgccgcg acgccggagg gccagcccga cttcatcgtc gacaagcacg ggtttcgcag
     1861 ccgctacacc acggcggagg tactggagga gatcgagcgc gtggccgggg atctgcgcga
     1921 gcggatcgcc gagctgctgc gcaagcgcgg tctgcgggcc gcggcggtca gcggcgccga
     1981 gacgcggctg ctgcacgccg aggtgcgcca gaacatgtgg tgggtcggcg acgagctggt
     2041 cccgctgacc aacctcgtcg gcccggtgcg cgaggtcgac cgcgcgcggc tcgacgtgct
     2101 gctggcggag agcgacgcgg tcgtggtgag cccgctcgcc gtgaccgccg agcacccgcg
     2161 cgtaaacacc gacgccgacc gcgccgccgc ggcgatcgcc ggagccggcg acgccgagcg
     2221 cctcgcgctg gtgatggacg ccgaggcggt gctcgccgac ggcgagccgg tgcgggccgc
     2281 cgcgcgcgcg gacctgaagg agctcgcccg ccacgcgacc ggcggcatgt cgaagaagct
     2341 gggcgccgtc gaggaggcct tcgccggcgg cgtccgcgag gtcgcgatcg gcagcgccac
     2401 cgttcccgac ctgctcgccg gccgcgccgg cacgctgatc accgccgagc ccgacccctc
     2461 ccccgcccga tgagggattt gcatcgcgtg gcggccacaa tccttcatcg gagcgggtgt
     2521 gacgcagatg gcggctgagc tgcagcaggt gccgcccgcg atggcggagc tgcgggccga
     2581 ggcgcgggcg gcgtgcccgg acgggctgct cgacctgagc cgctccgagc cgttgccgat
     2641 cccgctcacg gacgactgcg cggcgcgcgt ggcggagcag ttcatgcgtg cgcggcagta
     2701 ctcaccgtcg gcgggcttga cggcggttcg cgacgccgcg gcgcgctacg cgagcgtgcg
     2761 gaccgagctc gagctcgacg ccgccgacgt cgtcatcacc gcaggggcga tggccggact
     2821 ggcggtcgcg ctgagggcgc tgatcgagcc tggtgacgag gtcctcgtcc cggtgcccta
     2881 cttccactcc tacccgaggc aggtgtcgat gctaggcggg atcccccgcc tcgtcgacac
     2941 acgcccgcgg cagggggagc tgacggcgca ggcgatcgag cgcgagctga ccccgcagac
     3001 acgggcgctg ctgctctgca acccggccaa tccggccgga gtggtgctcg accgcgagcg
     3061 gctcaccgcg atcctggccg cgctgccgcc gagcgtcgcg gtaatcgccg acgaggtcta
     3121 cgcggagtat gtctacggcg atgccgcgcc tcgcgagctc gcgggcggcg acggcgagcg
     3181 tcgcgagctc gtgggaccgg ccggcaacag cacgacgggt ccttcgggcg atcggctcgt
     3241 cccgcgcgcg acggacttcg tcagtgcact cgccgtggtc gccgacgagg acgcgggccg
     3301 cgaggtgatc gcgctcgaca ccgcgtcgaa gaccgccgcg atgcccggct ggcgagtcgg
     3361 caccgcgatc gcgaccgccg gcctggcacc gcgcctcgcc caggcggcgg cgacgctgag
     3421 cggcgcgccg aacacgctcg cccagctcgc gttcgccgcc tggctcgacg acccgccgca
     3481 gctcgaccgg atggcgccct accggccacg gctgcgcgac gtgctcgagg agctcgacga
     3541 ttccggcctg accgtccgcc caccggacgg cagctactac gtctggctca cagcccccgc
     3601 cggcgaccgg cccaccgccg gtcggtc
//
LOCUS       DATABV010000052         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2667255, whole genome shotgun sequence.
ACCESSION   DATABV010000052 DATABV010000000
VERSION     DATABV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2667255"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VHF88_02120"
     CDS             <1..697
                     /locus_tag="VHF88_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2160594.1"
                     /translation="SRGVPPTPRGTRTYGIDQGDLHAQEVTLRPMGAHFTVDDTPVEL
                     NVPGAHNVLNALAALAACRAAGVPIAEAAPAIAGFRGTGRRFEPRGSTATGALVYDDY
                     AHHPTEVRATLEAARTLDARRIVACFQPHLFSRTRELAAEFGRALAVADVIAVIDVYP
                     ARERAADFPGVSGLLVAEAAADAAPGRPVWWLPQMDDAERMLRDRLGPGDVLLTLGAG
                     DVDKLATRLTEAA"
     gene            694..1608
                     /gene="murB"
                     /locus_tag="VHF88_02125"
     CDS             694..1608
                     /gene="murB"
                     /locus_tag="VHF88_02125"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX2160595.1"
                     /translation="MNPPAGVERNRPLERLTTVRTGGPADWFARPDSDERLAELLAWA
                     DGEGIDVGVVGSGSNLLVADDGFRGLVIKLDGELARIDRDGDRMLCGGGARLPKASAR
                     AAQEGLTGLEFGVNIPGTVGGAVKMNANAYGGDLSRVLESVDVATPGGTERRVPAELG
                     FSYRRSNLAPREIVARAVFKLDPADPGEVKATLADMRRRRREAQPSGIKTFGSTFKNP
                     GDPRAEGRSAGVLLDGAGCRGLAVGGARFSDKHANFVENAGEATTADVVALMAEGRRR
                     VYERFGVALEPEVQLLGDVDADALYGTP"
     gene            1605..2441
                     /locus_tag="VHF88_02130"
     CDS             1605..2441
                     /locus_tag="VHF88_02130"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX2160596.1"
                     /translation="MKPPAVALRRPAASIARLRAKLTPGPRTRRRMLAAAVVAALLVA
                     LYMLWLRDSSLVAVEQVTVTGLTSRDADRVRVALASTAETMTTLNVDERRLRDAAAAF
                     PIVARIEVAPDFPNGMRIDVVEHRPAAIALADGRKQPVAGDGSVLAGMPVEGDLPEIE
                     LAVAMPQRQLGPGAARDCARVAGAAPAVIARRVDSLAREGGARGVVAQLEDGTELVFG
                     TPDRLAAKWAAAIRVLADDEAEGAVYVDVRIPERPVAGGLAAATVTPAAPLETAPTYP
                     QP"
     gene            2665..3750
                     /gene="ftsZ"
                     /locus_tag="VHF88_02135"
     CDS             2665..3750
                     /gene="ftsZ"
                     /locus_tag="VHF88_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2160597.1"
                     /translation="MDTGSYLAVIKVVGIGGGGTNAVNRMVDAGLKGVEFIAVNTDAQ
                     ALQMCDADIKLQIGDQLTRGLGAGAEPEVGYGAANESRDEIKEALKGADMVFVTAGEG
                     GGTGTGAAPVVAEVAKQEIGALTVGVVTRPFDFEGSQRKRQSDEGIDRLREQVDTLIV
                     IPNEKLLAVVERRTSILEAFREADNVLRQGVQGITDLITIPGLINLDFADVRTIMQDA
                     GSALMGIGEAGGENRAAEAARHAISSPLLEQSVEGATGILLNITGGSDLGLFEVNEAA
                     EIIQGATDQNSNIIFGAVVDDGLDDNVRVTVIATGFEGAKSRPLFSEDSTGRRREPRR
                     RDVSMDDNQRSSLEISDDDIDIPSFLK"
BASE COUNT          567 a         1438 c         1358 g          510 t
ORIGIN      
        1 ctcccgcggc gtaccaccaa cgccccgcgg cacccggacc tacggcatcg accaaggaga
       61 cctccacgcc caggaagtca ccctccgtcc catgggcgca cacttcacag tcgacgacac
      121 ccccgtcgag ctcaacgtcc ccggcgccca caacgtcctc aacgccctcg ccgccctcgc
      181 cgcctgccgc gcagccggcg tgccgatcgc cgaggccgcg cccgcgatcg ccggcttccg
      241 cggcaccggc cgccgcttcg agccccgcgg gtcgaccgcg accggcgcgc tcgtctacga
      301 cgactacgcc caccacccga cggaggtccg cgcgaccctc gaggcggcgc gcaccctcga
      361 cgcgcgccgg atcgtcgcct gcttccagcc gcacctcttc tcccgcactc gcgagctggc
      421 cgccgagttc ggacgtgccc tggcggtcgc cgacgtcatc gccgtgatcg acgtctatcc
      481 cgcacgcgag cgggcggcgg acttcccggg cgtcagcggg ctgctggttg ccgaggcggc
      541 cgcggacgcc gcgcccgggc ggccggtctg gtggctgccc cagatggacg acgccgagcg
      601 gatgctgcgc gaccggctgg ggcccggcga cgtgctgctg accctcggcg ccggcgacgt
      661 cgacaagctc gcgacccgtc tgacggaggc cgcgtgaacc cgccggcggg ggtcgagcgc
      721 aaccggccgc tggagcgcct gaccactgtc cgcacgggtg gtccggccga ctggttcgcc
      781 cgtcccgaca gcgacgagcg gctggccgag ctgctcgcct gggccgacgg cgaggggatc
      841 gacgtaggag tggtcggctc gggatcgaac ctgctggtag ccgatgacgg atttcgcgga
      901 ctggtcatca agctcgacgg cgagctcgcg cggatcgacc gcgacggcga ccgcatgctc
      961 tgcggcggcg gcgcccgcct accgaaggcc tcggcacgcg ccgctcagga gggcctcacc
     1021 gggctcgagt tcggcgtcaa catcccgggc accgtcggcg gcgcggtgaa gatgaacgcc
     1081 aacgcctacg gcggcgacct gtcgcgcgtg ctcgagtcgg tcgacgtggc gacgcccggc
     1141 ggcaccgagc gccgcgttcc agccgagctc gggttctcct accggcgctc gaacctcgct
     1201 ccgcgcgaga tcgtcgcccg cgcggtcttc aagctcgatc cggccgaccc gggggaggtc
     1261 aaggcgacgc tcgccgacat gcgtcgccgg cggcgcgagg ctcagccgtc ggggatcaag
     1321 accttcggct cgaccttcaa gaacccgggc gatccgcgtg ccgagggccg cagcgccggt
     1381 gtcctgctcg acggcgcggg ctgccgcggc ctggccgtcg gcggcgcgcg cttctcggac
     1441 aagcacgcca acttcgtcga gaacgccggc gaggcgacga cggcggacgt cgtcgctctg
     1501 atggcagagg gccgccggcg cgtctacgag cgcttcggcg tcgcgctcga gcccgaggtc
     1561 cagctgctcg gcgacgtcga cgcggacgcc ctctacggga cgccatgaag ccgccggccg
     1621 tcgcccttcg ccgtcccgcc gcctcgatcg cccgtctgcg cgcgaagttg actcccggcc
     1681 cgcgcacccg tcgacgcatg ctggcggcgg ctgtcgtggc ggcgctgctg gtcgcgctct
     1741 acatgctctg gctgcgcgac tcctcgctgg tggcggtcga gcaggtgacg gtgacggggc
     1801 tgaccagtcg cgacgccgat cgcgtccgcg tcgcgctcgc ctcgaccgcc gagacgatga
     1861 cgacgctgaa cgtcgacgag cggcggctgc gcgacgcggc ggcggcattc ccgatcgtcg
     1921 cgcggatcga ggtcgcgccc gacttcccga acggaatgcg gatcgacgtg gtcgagcacc
     1981 ggccggcagc gatcgcgctc gccgacgggc gcaagcagcc ggtcgccggc gacggctcgg
     2041 tgctcgccgg catgccggtc gagggcgacc tgcccgagat cgagcttgcc gtggcgatgc
     2101 cgcagcgcca gctcgggccc ggagcggcgc gcgattgcgc gcgcgtggcg ggggcggcgc
     2161 cggcggtgat cgcccggcgc gtcgacagcc tcgcgcgcga gggcggcgcc cgcggcgtcg
     2221 tggcccagct cgaggacggc accgagctcg tcttcggcac tcccgaccgg ctcgcggcga
     2281 agtgggcggc ggcgattcgc gtgctcgccg acgacgaggc ggaaggcgct gtctacgtcg
     2341 acgtccgcat ccccgagcgg ccggtcgcag ggggtctcgc agccgcgacc gtcacccccg
     2401 ccgcgcccct cgaaacggcc ccgacatacc ctcaaccatg agttgagagt ccgcgcaact
     2461 ctcgaccctc gctagagggt cagggtgcaa gggacgttgc atccccggtt tgacattttc
     2521 cgcgaagtcc acctacagtg ctggtttgca gcgattcctc ggtggcggcg caaaccctcg
     2581 atgatcgctt cacctccgga cgcaaacgtc cgatagggag cttcacgaca gcgaacccga
     2641 gcaggcgagg caaggattca agcgatggac accggtagct acttggccgt catcaaggtc
     2701 gtcggaatcg gcggcggcgg cacgaacgcc gtcaaccgga tggtcgacgc cggacttaag
     2761 ggcgtcgagt tcatcgcggt caacaccgac gcgcaggcgc tccagatgtg cgacgccgac
     2821 atcaagctgc agatcggcga ccagctcacg cgtggcctcg gggccggggc cgagccggag
     2881 gtcggctacg gcgccgccaa cgagagccgc gacgagatca aggaggcgct caagggcgct
     2941 gacatggtct tcgtcaccgc cggcgagggc ggcggcaccg gcaccggcgc tgcgcccgtc
     3001 gtcgccgagg tcgccaagca ggagatcggc gcactgaccg tcggcgtcgt cacccggccg
     3061 ttcgacttcg agggctcgca gcgcaagcgc cagtccgacg aggggatcga ccgcttgcgc
     3121 gagcaggtcg acacgctgat cgtgatcccg aacgagaagc tgctcgccgt cgtcgagcgc
     3181 cgcaccagca tcctcgaggc cttccgcgag gccgacaacg tgctgcgcca gggcgtccag
     3241 gggatcaccg acctgattac gatccccggc ctgatcaacc tcgacttcgc cgacgtccgc
     3301 acgatcatgc aggacgccgg ctccgcgctg atggggatcg gcgaggccgg aggcgagaac
     3361 cgcgccgccg aggccgcccg ccacgcgatc tcgagcccgc tgctcgagca gtcggtcgag
     3421 ggcgccaccg ggatcctgct gaacatcacc ggcggcagcg accttggcct gttcgaggtc
     3481 aacgaggcgg ccgagatcat ccagggcgcc accgaccaga actccaacat catcttcggc
     3541 gccgtcgtcg acgacggcct cgacgacaac gtccgcgtca cggtgatcgc gaccggcttc
     3601 gagggcgcca agtcgcggcc gctgttcagc gaggactcga ccggccggcg ccgcgagccg
     3661 cgccgccgcg acgtctcgat ggacgacaac cagcgctcct cgctcgagat ctccgacgac
     3721 gacatcgaca tcccgtcgtt cctgaagtag cgagcgtcct ccagcgcgcg gcgggctccc
     3781 gctgccgagc gttacggtga gcccgtgact cggcagaggg ctgcaaccga tttcgacccc
     3841 gcgcggtact gggactcgcg tcatgagatc gag
//
LOCUS       DATABV010000053        10016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_338349, whole genome shotgun sequence.
ACCESSION   DATABV010000053 DATABV010000000
VERSION     DATABV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338349"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..578
                     /gene="rplP"
                     /locus_tag="VHF88_02140"
     CDS             156..578
                     /gene="rplP"
                     /locus_tag="VHF88_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX2160598.1"
                     /translation="MLQPKRVKHRKQHRGRRAGYSRGGTAVEFGEYGLKTTERGWITN
                     RQIEAARIAMTRKIKRGGKVWINIFPDKPVTKKPAETRMGSGKGSPEGWVAVVKPGRV
                     MFELSGVPEDLAREAMRLAGHKLPVKTKFVMREGAGQQ"
     gene            575..802
                     /gene="rpmC"
                     /locus_tag="VHF88_02145"
     CDS             575..802
                     /gene="rpmC"
                     /locus_tag="VHF88_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX2160599.1"
                     /translation="MKAVEVHNLDDPQLVEFVDNARKETFNLRFQHATGQLENTAGMK
                     NAKKDLARALTVARERGLDIEAEIRKQQRND"
     gene            1134..1367
                     /gene="rpsQ"
                     /locus_tag="VHF88_02150"
     CDS             1134..1367
                     /gene="rpsQ"
                     /locus_tag="VHF88_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX2160600.1"
                     /translation="MRQGVVVSDKADKTITVRIDVARPHRTYGKIVRSTSTLHAHDER
                     NEAGAGDTVRVVESRPLSRTKRWRLVEVVERAR"
     gene            1364..1732
                     /gene="rplN"
                     /locus_tag="VHF88_02155"
     CDS             1364..1732
                     /gene="rplN"
                     /locus_tag="VHF88_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX2160601.1"
                     /translation="MIQQETRLRVADNTGAREILCIRVAGGSKRRYAGVGDVITATVK
                     QAIPHGSVKKGEVVKAVVVRTRKQFGREDGTYISFDENAAVLIDQANNPRGTRIFGPV
                     ARELRDRNFMKIVSLAPEVL"
     gene            1744..2073
                     /gene="rplX"
                     /locus_tag="VHF88_02160"
     CDS             1744..2073
                     /gene="rplX"
                     /locus_tag="VHF88_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX2160602.1"
                     /translation="MKIRKDDTVKVISGKDRGKTGTVLRTDPERQRVYVQGLNIQKRH
                     LRPQQLQDTQRASQVEGGVIEVEGPIHVSNVQLLDPSSGDPTRVGIQREDGRRKRVAK
                     RSGTVID"
     gene            2073..2681
                     /gene="rplE"
                     /locus_tag="VHF88_02165"
     CDS             2073..2681
                     /gene="rplE"
                     /locus_tag="VHF88_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX2160603.1"
                     /translation="MPVATAKVTPRLKERYQQEIVPVLVERFGYANPMQVPKVEKVTL
                     NMGVGDAKQDSRALESAQGQLAAIAGQRPNIRRARKSIAAFKLREEMPVGLSVTLRDA
                     RMYEFLDRLITIAIPRIRDFRGLNARSFDGRGNWTMGVREQIIFPEIDYDGIDQVRGL
                     DVTITTTANTDEEAFALLHELGMPFRADGRPGEQRKTDEDGD"
     gene            2683..2868
                     /locus_tag="VHF88_02170"
     CDS             2683..2868
                     /locus_tag="VHF88_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX2160604.1"
                     /translation="MAKTSKRVSQQRPSKYKTRDYNRCRKCGRPRAYLRKFGLCRICV
                     RAHAHEGDIPGLTKSSW"
     gene            2897..3295
                     /gene="rpsH"
                     /locus_tag="VHF88_02175"
     CDS             2897..3295
                     /gene="rpsH"
                     /locus_tag="VHF88_02175"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX2160605.1"
                     /translation="MSLNDPIADFLTRVRNGLQADHDSVVMPHSRFKTELARILSEQG
                     YVEGYSVEPGRVGQQLRVTLKYTESRDPVISGMERVSKPGRRQYVGANEVPKVQGGMG
                     TTIVSTSRGVMTGHQARKAGVGGELVAKVW"
     gene            3299..3832
                     /gene="rplF"
                     /locus_tag="VHF88_02180"
     CDS             3299..3832
                     /gene="rplF"
                     /locus_tag="VHF88_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX2160606.1"
                     /translation="MSRIGKQPIPVPDGVSVEIAPEQVSVKGPKGELSERVARDIEIV
                     QEDGTVVVRRPSDRGEHRALHGLTRTLIYNMVAGVTEGFEKRLEIQGVGYRAQLKGKN
                     LELALGYSHPVAIEAPENVEFEVPQPTQVIVRGISKQVVGETAARIRKQRPPEPYKGK
                     GIRYEGEYVARKVGKRA"
     gene            3829..4182
                     /gene="rplR"
                     /locus_tag="VHF88_02185"
     CDS             3829..4182
                     /gene="rplR"
                     /locus_tag="VHF88_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX2160607.1"
                     /translation="MSTVTKGSKRLRRRRRVRAKVRGTAERPRLSVFRSNRGVMCQLV
                     DDERGHTLAAVNWTEAELRDLPRIEAAKRAGELLGERAKQAGIETCVFDRGGYRYHGH
                     VKAVAEGAREAGLVF"
     gene            4207..4725
                     /gene="rpsE"
                     /locus_tag="VHF88_02190"
                     /pseudo
     CDS             4207..4725
                     /gene="rpsE"
                     /locus_tag="VHF88_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            4857..5042
                     /gene="rpmD"
                     /locus_tag="VHF88_02195"
     CDS             4857..5042
                     /gene="rpmD"
                     /locus_tag="VHF88_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2160608.1"
                     /translation="MAATLEITQVKSSNGASKKQLDSLRTLGLGRIGKQVQRPDDAVV
                     RGLVASVDHLVKVGDDG"
     gene            5056..5529
                     /gene="rplO"
                     /locus_tag="VHF88_02200"
     CDS             5056..5529
                     /gene="rplO"
                     /locus_tag="VHF88_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX2160609.1"
                     /translation="MGLHNLKPAKGSTHRRKRVGRGEGSGVGKTSGRGQKGAGARAGS
                     KTRVAFEGGQNPIQRRMRKLRGPHMKKSMPFEKFRTHTQPVNLRDLDARFDAGADVTP
                     DSLRAAGLATRRNVPIKVLGEGELEKALTVHAHGFSASAREKIESAGGSVNVLES"
     gene            5533..6801
                     /gene="secY"
                     /locus_tag="VHF88_02205"
     CDS             5533..6801
                     /gene="secY"
                     /locus_tag="VHF88_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2160610.1"
                     /translation="MLKTFLNAFRIPELRKKLAFTAAMLALYRLGAYIPAPGINVDAV
                     EALSEQFAGSNILGFLNLFSGGSLQRFAIFALGIMPYITASIILQLMTVVLPSLEKLR
                     KEGEVGQQKITQYTRYLTVALAFGQSVGYVFLFRSFSTGTTDVVDGFTFGTVFTIIIT
                     LTAGCVLLMWFGELITQRGIGNGISLMIFASIVAGLPNGVSAWWTNPDQVFKVMMPFL
                     ALAIIAGIVFMQEGQRRIPVQYAKQVVGRRIASASSTYLPLRVNMAGVIPVIFAASLM
                     AFPPTVGQLIQSPWAQDMSAFFSPNGWAYIVGESILIVLFTYFYTAVTFNPVEQADNL
                     KKYGGFIPGVRPGRPTAEYLDRILARLTFPGALYLAAVAALPTILINQTSANFFFGGT
                     SILIVVGVALDTMKQLEAQMMMRNYEGFLK"
     gene            6814..7488
                     /locus_tag="VHF88_02210"
     CDS             6814..7488
                     /locus_tag="VHF88_02210"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2160611.1"
                     /translation="MSELNLILLGPPGAGKGTQAERLVEDFDLPYYATGDILRGAVKE
                     GTELGRQAKQYMDRGDLVPDEVICGVVVERIDSPEALDGFLLDGFPRTLPQAEALERS
                     LDPLGRRLTAVLSIEVADDELVRRLSGRRVCERNGHPYHVEFDPPKNAGFCDQDGSPL
                     IQRDDDREETIKHRLDVFHEQTEPLVDLYEAKGLLRRFDGSRSPDEVHAHIRATLATL
                     RLEEQL"
     gene            7491..8294
                     /gene="map"
                     /locus_tag="VHF88_02215"
     CDS             7491..8294
                     /gene="map"
                     /locus_tag="VHF88_02215"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2160612.1"
                     /translation="MAIIRKTPEEIERIAASGRVLARCHRLLRAKARAGVTTAELDEA
                     AERFIRSQGATPAFKGYRGFPGSICASPNSMVVHGIPGAYELSRGDVLAIDIGVELDG
                     WISDAAVTLPIGPVSDVAAGLLEATRASLFDGIEQCVPGNRLGDVSAAIQRRVESDGY
                     SVIRSLVGHGVGREMHEDPQIPNFGTPGTGPLLEEGMVLAIEPMVNVGSADVRLAPDN
                     WSVYSQDGSLAAHFEHTVAVTADGPRILTPWHIDEGTASPVRAKAASGH"
     gene            8393..8506
                     /gene="rpmJ"
                     /locus_tag="VHF88_02220"
     CDS             8393..8506
                     /gene="rpmJ"
                     /locus_tag="VHF88_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX2160613.1"
                     /translation="MKVRASVKPMCEKCKVIRRNGAVLVICQNPRHKQRQG"
     gene            8515..8895
                     /gene="rpsM"
                     /locus_tag="VHF88_02225"
     CDS             8515..8895
                     /gene="rpsM"
                     /locus_tag="VHF88_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX2160614.1"
                     /translation="MARIAGINIPLNKRVEIGLTYIYGIGRSQSNVILKKAGVDPDTY
                     VKDLTDDEVIKLRDAIDQDLTVEGDLRRELSQNIKRLMEIGAYRGLRHRRSLPVRGQR
                     TKTNGRTRKGPKRMQVAGKKKVTK"
     gene            8895..9293
                     /gene="rpsK"
                     /locus_tag="VHF88_02230"
     CDS             8895..9293
                     /gene="rpsK"
                     /locus_tag="VHF88_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX2160615.1"
                     /translation="MAQQRPGRPRGRRKAKKNVPFGQAHIKTSFNNTIVSLTDREGNV
                     IAWESAGGAGFKGSRKSTPFAAQVTADAAAKKGIEQGLEKVEVFVKGPGSGRDTAIRS
                     LQAAGLEVTGIRDVTPQAHNGCRPRKRRRV"
     gene            9297..9917
                     /gene="rpsD"
                     /locus_tag="VHF88_02235"
     CDS             9297..9917
                     /gene="rpsD"
                     /locus_tag="VHF88_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX2160616.1"
                     /translation="MARDTGAQCKQCRREGTKLFLKGERCLTDKCGIERRNYPPGDHG
                     RGRPRQSEYRVQLREKQKARRYYGVLEKQFRGYYDRASRQEGVTGENLLRILECRLDN
                     VLVRLGFAASRRQARQFVRHGQWLVNGRRVDIPSYQVRPDDVITLAPGSKGDQTIRDA
                     TELVSVVPAWLQADHDGLMAKVLRRPERDEIAVPVQEQLIVELYSK"
BASE COUNT         1741 a         3468 c         3441 g         1366 t
ORIGIN      
        1 ggaggtcgtg gcggtccggg tggacgcggt ggcggtcctg gcggacgggg cggcggtccc
       61 ggcggacgcg gtggcggtcc cggcggacgg ggcggcggtc ctggcggacg cggtggcggt
      121 cccggcggac ggggcggcgg aagggggcag cgctgatgct gcagcccaag cgcgtaaagc
      181 accgcaagca gcaccgtggt cgcagggccg gctactcgcg tggcgggacg gccgtcgagt
      241 tcggcgagta cggcctcaag acgaccgagc gcggctggat caccaaccgc caaatcgagg
      301 ccgcccgtat cgcgatgacg cgcaagatca agcgcggcgg caaggtctgg atcaacatct
      361 tccccgacaa gccggtcacc aagaagccgg ccgagacccg catgggctcc ggcaagggct
      421 cgcccgaggg ctgggtcgcc gtggtcaagc cgggtcgcgt gatgttcgag ctctcgggcg
      481 tccccgagga cctcgcccgc gaggcgatgc ggctcgccgg tcacaagctg cccgtcaaga
      541 cgaagttcgt gatgcgcgag ggggccggac agcagtgaag gccgtcgagg tgcacaatct
      601 ggacgacccg cagctcgtcg agttcgtcga caacgcgcgc aaggagacgt tcaacctgcg
      661 tttccagcac gcgaccggac agctcgagaa caccgcgggt atgaagaacg ccaagaagga
      721 cctggcgcgc gcgctgaccg ttgcgcgcga gcgcggtctg gacatcgagg cagagatcag
      781 gaagcagcag cgcaatgact gacgacaaca acgcgaacga cgacaacgtc gacgagaccg
      841 ccgaggctcc cgcggccgcc gaggggaccg cagccgcggc tcctgtggag gacgccgagc
      901 ccgaggtagc gctcgatccc aagcagcgcc gcaagcgcgc gcgctcgctg aaccgcggcg
      961 aggcacgccc gcagcggtcg gtcgaggagc gcacggccga gcggctcgag cgccggcgca
     1021 agaaggccgt cgcccgccgc acctggcgcg tcaagagccg cgagcgccgt cgcgcggcca
     1081 gctccggcca gggaacgccg ccggctgaga aggcgaccac cggcagcccg cgcatccgcc
     1141 agggagtcgt ggtctccgac aaggccgaca agacgatcac cgtccgcatc gacgtcgcgc
     1201 gcccgcaccg cacctacggc aagatcgtcc gctcgacctc gacgctgcac gcgcacgacg
     1261 agcgcaacga ggccggcgcg ggggacaccg tgcgtgtcgt cgagagccgt ccgctgagcc
     1321 gcaccaagcg ctggcgcctg gtcgaggtcg tggagcgtgc gcggtgatcc agcaggagac
     1381 ccgcctcagg gtggccgaca acaccggcgc ccgcgagatc ctctgcatcc gcgtcgcggg
     1441 tggcagcaag cggcgctacg ccggcgtcgg cgacgtgatt accgcgaccg tcaagcaggc
     1501 gattccgcac ggctcggtca agaagggcga ggtcgtcaag gcggtcgtcg tccgcacgcg
     1561 caagcagttc gggcgcgagg acggcaccta catttccttc gacgagaacg ccgcggtgct
     1621 gatcgaccag gccaacaacc cgcgtgggac gcgcatcttc ggcccggtcg ctcgcgagct
     1681 gcgcgaccgc aacttcatga agatcgtctc gctggcgccg gaggtcctct gatggcgtcg
     1741 cgcatgaaga tccgcaagga cgacaccgtc aaggtgatct ccggcaagga tcgcggcaag
     1801 accggcaccg tgctgcgcac cgatccggag cgtcagcgcg tctacgtcca gggcttgaac
     1861 atccagaagc gccacctgcg tcctcagcag ctgcaggaca cccagcgcgc ctcgcaggtc
     1921 gagggcggcg tgatcgaggt cgagggcccg atccacgtct ccaacgtcca actgctcgat
     1981 ccgagcagcg gcgatccgac gcgagtcgga atccagcgcg aggacgggcg gcgcaagcgc
     2041 gtggcgaagc gctcagggac ggtgatcgac tgatgccggt cgctaccgcg aaggtcacgc
     2101 cgcgcctcaa ggagcgctac cagcaggaga tcgtgccggt cctggtcgag cgcttcggct
     2161 acgccaaccc gatgcaggtg ccgaaggtcg agaaggtcac gctcaacatg ggcgtcggcg
     2221 acgcgaagca ggacagccgc gcgctcgagt cggcccaggg gcagctcgcc gcgatcgccg
     2281 gacagcggcc gaacatccgc cgcgcgcgca agtcgatcgc cgccttcaag ctgcgcgagg
     2341 agatgccggt cgggctctcg gtgacgctgc gggacgcgcg gatgtacgag ttcctcgatc
     2401 gcctgatcac gatcgcgatc ccgcggatcc gcgacttccg cgggctcaac gcgcgctcgt
     2461 tcgacgggcg cgggaactgg acgatgggcg tccgcgagca gatcatcttt cccgagatcg
     2521 actacgacgg gatcgaccag gtgcgcggtc tcgacgtcac gatcacgacg accgcgaaca
     2581 ccgacgagga ggccttcgcg ctgctgcacg agctcggaat gccgttccgg gccgacggcc
     2641 gcccgggcga gcagcgcaag accgacgagg acggagactg agatggccaa gacttccaag
     2701 cgggtgagcc agcagcgccc atccaagtac aagacccgtg actacaaccg ctgccgcaag
     2761 tgcggccggc cgcgcgccta cctgcgcaag ttcggcctct gccggatctg cgtgcgcgcg
     2821 catgcccacg agggcgacat ccccggcctg accaagtcga gctggtagcg gacgagcaga
     2881 acgagagacg atcgccttga gcctcaacga cccaatcgca gacttcctga cgcgtgtgcg
     2941 caacggcctc caggccgacc acgactctgt cgtgatgccg cattcgcgct tcaagacaga
     3001 gctcgcacgc attctcagcg agcagggcta cgtcgagggc tactcggtcg agccgggtcg
     3061 ggtcggtcag cagctgcgcg tcacgcttaa gtacaccgag agccgcgatc cggtcatctc
     3121 cggcatggag cgcgtctcca agcccgggcg ccgccagtac gtcggcgcga acgaggtgcc
     3181 gaaggtccag ggcgggatgg gtacgacgat cgtctccacc tcgcgtgggg tcatgacggg
     3241 gcaccaggcg cgcaaggcgg gcgtcggcgg ggaactggtg gcgaaggtct ggtagtcgat
     3301 gagtcgcatc ggaaaacagc cgatcccagt gcccgacggc gtgtccgtcg agatcgcccc
     3361 tgagcaggtc agcgtcaagg gcccgaaggg cgagctgtcc gagcgggtcg cccgcgacat
     3421 cgagatcgtc caggaggacg ggacggtggt ggtccgccgt ccctccgacc gcggcgagca
     3481 tcgcgcgctg cacggcctga cccggacgct gatctacaac atggtcgccg gcgtgaccga
     3541 gggcttcgag aagcggctcg agatccaggg cgtcggctat cgcgcccagc tcaagggcaa
     3601 gaacctcgag ctggcgctcg gctactcgca tccggtggcg atcgaggctc ccgagaacgt
     3661 cgagttcgag gttccgcagc cgacgcaggt gatcgtccgc ggcatctcga agcaggtcgt
     3721 cggcgagacc gccgcgcgga tccgcaagca gcgcccgccg gagccctaca agggcaaggg
     3781 catccgctac gagggcgagt acgtcgcgag gaaggtcggc aagcgcgcat gagcaccgtc
     3841 accaagggca gcaagcgact gcgccggcgc cgccgggtgc gcgccaaggt gcgtggcacc
     3901 gccgagcgac cgcgcctgtc ggtcttccgc tccaaccgcg gcgtcatgtg ccagctcgtc
     3961 gacgacgagc gcggtcacac gctcgcggcc gtcaactgga ccgaggccga gctgcgcgat
     4021 ctgccgcgca tcgaggcggc caagcgtgcc ggcgagctgc ttggcgagcg ggccaagcag
     4081 gccgggatcg agacctgcgt cttcgatcgc ggcggctacc gctaccacgg acacgtcaag
     4141 gcggtcgccg agggggcgcg cgaggcggga ctcgtcttct agctaccctg cgacaggaac
     4201 tttcacatgg cacgtaacga caacagacag ggcggccgcg gcggtcgcga cagcgatctc
     4261 caagagcggg tcgtcgagat caaccgcgtc gcgaaggtcg tcaagggcgg ccggcgcttc
     4321 tcgttcactg cgctcgtcgt cgtgggcgac gagaagtcga aggtcggcgt cggctacggc
     4381 aaggcgaacg aggttccggt cgcgatccag aagggcgtcg agcgcgcccg ccgcaacctc
     4441 gtcgaggtgc cgatgcacgg atcgacgatc acgcacaaga cgatcggcgt ccactccgcc
     4501 tcgcgcgttc tgctcaagcc ggcctcgccc ggtaccggcg tgatcgccgg cggcggcgtg
     4561 cgcgccgtgc tcgagctcgc gggaatccac gacgtgctgt cgaagtcgct tggcagccag
     4621 aacccgatca acctcgtcaa ggcgacgatg gcggcgctcg agtcgctgcg ccggccgcag
     4681 gacgtcgccg cgctgcgcgg cctttcgatc gccgaggtcc tcggccttga cgagaagccc
     4741 ggtgacgagg agccggcagc cgcggccgag gccgacgctt ccgacgcggc agaccagccc
     4801 gccgagcagt ccgagccggt ggccgccgcc gcgggagcgg agcaggagtc ctagcgatgg
     4861 ccgccacgct cgagatcacc caggtcaagt cgtccaacgg cgcgtcgaag aagcagctcg
     4921 actcgctgcg gacgctcggc ctcggccgga tcggcaagca ggtgcagcgg cccgacgacg
     4981 ccgtcgtgcg cggcctcgtc gcgagcgtcg accatctcgt gaaggttggc gacgatggct
     5041 gacgacgagc gcttcatcgg cctgcacaac ctcaagccgg cgaagggctc gacccatcgc
     5101 cgcaagcgcg tcgggcgcgg ggagggctcg ggcgttggca agacctccgg tcgtgggcag
     5161 aagggcgcag gcgctcgggc gggctcgaag acccgggtcg ccttcgaggg cgggcagaac
     5221 ccgatccagc ggcgaatgcg caagctgcgc ggcccgcaca tgaagaagtc gatgccgttc
     5281 gagaagttcc gtacgcacac gcagcccgtg aacctgcgcg acctcgacgc ccgcttcgac
     5341 gccggcgccg acgtgacgcc agactccctc cgcgcggccg gtctcgcgac ccgccgcaat
     5401 gttccgatca aggtcctagg ggagggagag ctcgagaagg cgctcaccgt ccatgcccac
     5461 ggcttctccg ccagcgcgcg cgagaagatc gagtcggcag gcggcagcgt caacgtcctc
     5521 gagtcgtaga gcatgctcaa gacgttcctc aacgctttcc ggatccccga gctccggaag
     5581 aagctggcgt tcaccgccgc gatgctcgcg ctctaccggc tcggcgccta catcccggcg
     5641 cccggcatca acgtcgacgc ggtcgaggcg ctgtcggagc agttcgcggg ctcgaacatc
     5701 ctcggcttcc tcaacctctt ctccggcggc agcctgcagc gcttcgcaat tttcgcgctc
     5761 gggatcatgc cctacatcac cgcctcgatc atcctgcagc tgatgacggt cgtgctgccg
     5821 tcgctcgaga agctgcgcaa ggagggcgag gtcggccagc agaagatcac gcagtacacg
     5881 cgctacctga cggtggctct cgccttcggc cagtcagtcg gctacgtctt cctcttccgc
     5941 agcttctcga ccggcacgac cgacgtcgtc gacggcttca ccttcggcac ggtcttcacg
     6001 atcatcatca cgctgaccgc cggctgcgtg ctgctgatgt ggttcggcga gctgatcacc
     6061 cagcgcggga ttggcaacgg catctcgctg atgatcttcg ccagcatcgt cgcggggctg
     6121 cccaacggcg tctcggcgtg gtggacgaac cccgaccagg tcttcaaggt gatgatgccg
     6181 ttcctggcgc tggcgatcat cgccggcatc gtcttcatgc aggagggcca gcggcgcatc
     6241 ccggtgcagt acgccaagca ggtggtcggg cgccggatcg cgtcggcgag ctcgacctac
     6301 ctgccgctgc gggtcaatat ggccggcgtc atccccgtga tcttcgccgc gtcgctgatg
     6361 gcgttcccgc cgactgtcgg ccagctgatc caatcgccct gggcgcagga catgtcggcg
     6421 ttcttcagtc cgaacggctg ggcgtacatc gtcggcgagt cgatcctgat cgtgctgttc
     6481 acctacttct acacagccgt cacgttcaac ccggtcgagc aggcggacaa cctgaagaag
     6541 tacggcggct tcattcccgg ggtcagaccc gggcggccga cggccgagta tctggaccgc
     6601 atcctggctc ggctgacgtt cccgggcgcg ctctacctcg cggcggtcgc ggccctgccg
     6661 acgatcttga tcaaccagac gagcgcgaac ttcttcttcg gagggacctc gatcctgatc
     6721 gtcgtcggcg tcgcgctcga cacgatgaag caactagagg cgcagatgat gatgcgcaac
     6781 tacgagggat tcttgaagta gcctccctcg tctgtgagcg agctcaatct catcctcctc
     6841 ggtcctcccg gcgccggcaa gggaacccag gccgagcggc tcgtcgagga cttcgacctg
     6901 ccctactacg cgaccggcga catcctgcgc ggcgcggtca aggagggaac cgagctcggc
     6961 cgtcaggcga agcagtacat ggaccgcggc gacctcgtcc ccgatgaggt gatctgcgga
     7021 gtcgtcgtcg agcggatcga cagccccgag gcgctcgacg ggttcctgct cgacggcttc
     7081 ccgcgcacgc tgccgcaggc cgaggcgctc gagcgctccc tcgacccgct cggccgccgg
     7141 ctgaccgcgg tcctctcgat cgaagtggcc gacgacgagc tcgtgcgccg cctgtccggg
     7201 cggcgcgtct gcgagcgcaa cggacatccg taccacgtgg agtttgaccc gcccaagaac
     7261 gccggattct gcgaccagga cggctcgccg ctgatccagc gcgacgacga ccgcgaggag
     7321 acgatcaagc accggctcga cgtcttccac gagcagaccg agccgctcgt cgacctctac
     7381 gaggccaagg gcctgctgcg ccgcttcgac ggcagccgct cccccgacga ggtccatgcg
     7441 cacatccgcg cgacgctcgc gacgctgcgc ctcgaggaac agctctagcg atggcgatca
     7501 tccgcaagac gcccgaggag atcgagcgga tcgcggcctc cgggcgggtc ctcgcgcgct
     7561 gccaccggct gctgcgcgcg aaagcgcgcg ccggcgtcac gacggccgag ctcgacgagg
     7621 ccgccgagcg gttcatccgc tcgcagggcg cgacccccgc cttcaagggc taccgcggct
     7681 tcccaggctc gatctgcgcg tcgccgaact cgatggtcgt ccacggcatc cccggcgcct
     7741 acgagctgag ccgcggcgac gtgctcgcga tcgacatcgg agtcgagctc gacggctgga
     7801 tctcagacgc ggccgtcacg ctgccgatcg ggccggtcag cgacgtcgcc gccggactgc
     7861 tcgaggcgac ccgagcctcg ctgttcgacg ggatcgagca gtgcgtgccg gggaaccgcc
     7921 tcggcgacgt ctcggccgcg atccagcgcc gcgtcgaatc cgacggctac tcggtgatcc
     7981 gctcgctggt cgggcacggg gtcgggcgcg agatgcacga ggacccgcag atccccaact
     8041 tcggcacacc cggcacagga ccgctgctcg aggaggggat ggtgctagcg atcgagccga
     8101 tggtcaacgt cggcagcgcg gacgtccgtc tggcccctga caactggtcg gtctactccc
     8161 aggacggctc gctggcagcc cacttcgagc acacggtcgc cgtgacggcc gacgggccgc
     8221 gcatactgac tccctggcac attgacgaag gcaccgccag ccccgtccga gcgaaggcgg
     8281 cgagcggtca ttaggccgag cgggcgctcc tgggccggcg tgctatcgtg ccccgttggc
     8341 gctgggccgt ctcctgggcc ctgcgctcgt tctctccata cggaaggtag cgatgaaggt
     8401 ccgagcttcg gtcaaaccca tgtgtgagaa gtgcaaggtc atccgccgca acggcgccgt
     8461 gctcgtgatc tgtcagaacc cgcgtcacaa gcagcggcag ggctgacccg gacgatggcg
     8521 cgcatcgcag gcatcaacat cccgctcaac aagcgggttg agatcggtct cacctacatc
     8581 tacgggatcg ggcgctcgca gtcgaacgtc atcctgaaga aggccggagt cgatcccgac
     8641 acctacgtca aggacctcac cgacgacgag gtcatcaagc tgcgcgacgc gatcgaccag
     8701 gacctcaccg tcgagggcga tctgcggcgc gagctgtccc agaacatcaa gcggctgatg
     8761 gagatcggcg cctaccgcgg cctgcgccac cgccgcagcc tgccggtccg gggccagcgc
     8821 acgaagacca acggtcgcac ccgcaagggc ccgaagcgca tgcaggtggc cggtaagaag
     8881 aaggtgacga agtagtggca caacagcgcc caggtagacc tcgcggccgc cgcaaggcga
     8941 agaagaacgt cccgttcggc caggcccata tcaagaccag cttcaacaac acgatcgtgt
     9001 cgctgaccga ccgtgagggc aacgtgatcg cgtgggagtc cgccggcgga gccggcttca
     9061 agggctcgcg caagtcgacc ccgttcgccg cccaggtcac cgccgacgcg gccgccaaga
     9121 agggcatcga gcaagggctc gagaaggtcg aggtcttcgt caagggcccc ggctccgggc
     9181 gcgacaccgc gatccgctcg ctgcaggcgg ccgggctcga ggtcacgggc atccgcgacg
     9241 tcacccccca ggcgcacaac ggctgccggc cgcgcaagcg gcggagggtc taggacatgg
     9301 cgcgagacac aggcgcacag tgcaagcagt gccgccgcga gggcaccaag ctcttcctca
     9361 agggcgagcg ctgcctcacc gacaagtgcg gcatcgagcg gcgcaactac ccgcccggcg
     9421 accacggccg cggccgtccg cgccagtccg agtaccgcgt ccagctgcgc gagaagcaga
     9481 aggcccgccg ctactacggc gtgctcgaga agcagttccg cgggtactac gaccgtgcgt
     9541 cgcgccaaga gggcgtgacc ggcgagaacc tgctgcggat cctcgagtgc cggctcgaca
     9601 acgtcctcgt ccggctcggc ttcgccgcct cgcggcgcca ggcgcgccag ttcgtccgtc
     9661 acgggcaatg gctcgtgaac ggccgccgcg tcgacatccc ctcgtaccag gtgcgtcccg
     9721 acgacgtgat cacgctggcc cccggctcaa agggcgacca gacgatccgg gacgcgaccg
     9781 agctggtctc cgtggttccc gcctggctgc aggccgacca cgacggcctc atggccaagg
     9841 tgctgcgccg gcccgagcgc gacgagatcg ccgtgccggt ccaagagcag ctcatcgtcg
     9901 agctttactc gaagtagaag tccaagccgc gcgcgacgcc cgccggcggt cgtgtgcgca
     9961 ccaacgcgaa ggacatcgcc catgatcgag ttccagaccc cccagatctc cgccga
//
LOCUS       DATABV010000054         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_338420, whole genome shotgun sequence.
ACCESSION   DATABV010000054 DATABV010000000
VERSION     DATABV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338420"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VHF88_02240"
     CDS             complement(<1..1589)
                     /locus_tag="VHF88_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2160617.1"
                     /translation="MPNPSLDGFDAPVRVRQVRWAAPDGGFAVLSVEGPDGDEVTVAG
                     PLAHLSEGDRARVVGRWAEHDRYGLQVQAEAAYELDPDDAAGALKYLTTISRIGKRRA
                     KQLVDRYGATVLEQIDADPDAAFRSLPRMSERAAAAAAESWRERRALRDLYVMLAPHG
                     AGWLAATLHERHGASAVGILRSDPYRLTEEHGVGFETADAIARAHGLAEDSPARYRAA
                     LVHVLQTAERRAGHTFLPVAELYRGATELVGQLPDALLDDLGAVHALVVENGRVALPA
                     TWESEAALADDLGGLAAAPAVPGPDWLAEAVEQLTDEQRRAVEAAFERRLSVVTGGPG
                     TGKTTLVSTIARLAERADLALALCAPTGRAARRLEEATGQPATTIHRLLEWIPHEGPL
                     RGPSYPIECDLLVVDESSMLSLDVARTLFDAVSADTAVVMVGDADQLPPIGAGKPFAD
                     LIESGSVPVARLTHVFRQSAQSMIVSAAHAVRHGEVPPVQPGEGQERDFFLLARDGEE
                     AAADAVVELATGRLARHYGVDP"
     gene            1603..1884
                     /locus_tag="VHF88_02245"
     CDS             1603..1884
                     /locus_tag="VHF88_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160618.1"
                     /translation="MSSPEAKNLEAISKAIDQHERSCEYPTLAILMNPFEVERLGWDQ
                     IRGIPVRPDEKIGTGRFEILCAGDGEEPELEESVEAVAEEARPLVVPLG"
     gene            1896..>2368
                     /locus_tag="VHF88_02250"
     CDS             1896..>2368
                     /locus_tag="VHF88_02250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2160619.1"
                     /translation="MAKRRENPPVEQLYAKRHKIFAVMMIGWAMSLLDVSIVNVAVPE
                     LQQELDTDVNSITWVVNAYNLSFAALLIAAGKLADQFGRRRFFILGLGIFTLGSFLCA
                     ISPSADVLIFSRVVQGAGAGILAPLGFAITAAVFPIQERGKALATIAIVALAASAS"
BASE COUNT          316 a          867 c          857 g          328 t
ORIGIN      
        1 gggtcgacgc cgtagtggcg ggcgagccgc ccggtcgcca gctcgacgac ggcgtccgcc
       61 gccgcctcct cgccgtcgcg cgcgagcagg aagaagtcgc gctcctggcc ctcgcctggc
      121 tggaccggcg ggacctcgcc atggcgaacc gcgtgcgcgg cgctgacgat catcgactgg
      181 gccgactggc ggaagacgtg cgtcagccgc gcgaccggca cgctgcccga ttcgatcagg
      241 tcggcgaacg gcttgccggc gccgatcggg gggagctggt cggcgtcgcc gaccatcacg
      301 accgcggtgt ccgcggagac cgcgtcgaag agcgtccgcg cgacgtcgag gctgagcatg
      361 ctcgactcgt cgacgacgag caggtcgcac tcgatcgggt agctcggccc gcgcagcggc
      421 ccctcgtgag ggatccactc gagcagccgg tggatcgtcg tcgccggctg tccggtcgcc
      481 tcctcgagcc gccgcgccgc gcggccggtc ggcgcgcaga gcgcgagcgc gagatctgcc
      541 cgctccgcga gccgggcgat cgtgctgacg agcgtcgtct tgccggttcc cggaccgccg
      601 gtgaccaccg acagccgccg ctcgaacgcc gcctcgacgg ccctgcgctg ctcgtcggtc
      661 aactgctcga cggcctcggc gagccagtcg gggccgggga cggccggcgc ggcagccagt
      721 ccgccgaggt cgtcggcgag cgccgcctcg gactcccacg tcgcaggaag cgcgacacga
      781 ccgttctcga ccaccaacgc atgcacggcg ccgaggtcgt cgagcagggc gtccggcagc
      841 tgcccgacca gctccgtcgc gccacggtac agctcggcga ccggcaggaa ggtgtggccg
      901 gcgcgccgct cggcggtctg caggacatgc acgagcgcgg cccggtagcg ggccggggag
      961 tcctcggcga ggccgtgggc acgggcgatc gcgtcggcgg tctcgaagcc gacgccgtgc
     1021 tcctcggtca ggcggtacgg gtcgctgcgc aggatgccta cggcgcttgc cccgtggcgc
     1081 tcgtggagcg tcgccgcgag ccagcctgcg ccgtgcgggg caagcatcac gtagaggtcg
     1141 cgcagcgcgc gccgctcgcg ccaggactcg gccgccgcgg ccgccgcccg ctcgctcatc
     1201 cgtggcagcg agcggaacgc cgcatccggg tcggcgtcaa tctgctcgag caccgtcgcg
     1261 ccgtagcggt cgacgagctg cttggcgcgg cgcttgccga tccggctgat cgtcgtcagg
     1321 tacttgagcg cgccggcggc gtcgtcggga tcgagctcgt aggccgcctc ggcctggacc
     1381 tggagtccgt agcggtcgtg ctcggcccag cgaccgacga cgcgcgcgcg gtcgccctcg
     1441 gagaggtgcg cgagcggccc ggcgaccgtg acctcgtcgc cgtccggccc ctcgaccgac
     1501 agcaccgcga agccgccgtc gggcgcggcc cagcgcacct gacgcacgcg gaccggcgcg
     1561 tcgaatccgt cgaggctggg attgggcata ctcgaaagac tgatgagctc tccggaggcc
     1621 aagaacctcg aggcgatctc caaggcgatc gaccagcacg agcgcagctg cgagtacccg
     1681 acgctggcga tcctgatgaa cccgttcgag gtcgagcggc tcggctggga ccagatccgc
     1741 gggatcccgg tccggccgga cgagaagatc ggcaccggcc gcttcgagat cctctgcgcc
     1801 ggcgacggcg aggagcccga gctcgaggag tcggtcgagg cggtcgcaga ggaggctcgt
     1861 cctctggtcg tccccctcgg ctagtctccc gcgccgtggc gaagcggcgc gagaaccctc
     1921 cggtggagca gctctacgcg aagcggcaca agatcttcgc cgtgatgatg atcggctggg
     1981 cgatgagcct gctcgatgtc tcgatcgtca acgtggcggt cccggagctg cagcaggagc
     2041 tcgacaccga cgtcaactcg atcacgtggg tcgtcaacgc ctacaacctg tccttcgccg
     2101 cactgctgat cgccgccggc aagctcgccg accagttcgg ccgccgccgg ttcttcatcc
     2161 tgggcctcgg catcttcacg ctcggctcgt tcctgtgcgc gatctcgccc tcggccgacg
     2221 tcctgatctt ctcgcgcgtc gtccagggcg cgggggcggg catcctcgcc ccgctcggct
     2281 tcgcgatcac cgccgccgtg ttcccaatcc aggagcgcgg caaggcgctc gcgacgatcg
     2341 cgatcgtcgc cctcgcggcg agcgcgtc
//
LOCUS       DATABV010000055         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2427000, whole genome shotgun sequence.
ACCESSION   DATABV010000055 DATABV010000000
VERSION     DATABV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427000"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VHF88_02255"
     CDS             complement(<1..1016)
                     /locus_tag="VHF88_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX2160620.1"
                     /translation="MAISAENIHFPSRTGSPARALGFRLLIALVLVGLVTAIAFADRG
                     GYRDADGSVSLLDCIYYATVSITTTGYGDIVPVSDTARLLTTFLVTPLRVLFLILLVG
                     TTLEFLASATRANLRRRIWRRKLHDHVVICGFGTKGRSALRSLIGTGTSLDQIVVIDE
                     RLEAIELATREGVTGVLGSAARRDVLTEAAAPDAAALIVATDRDDTSVLVTLTARELA
                     PNATITAAAREEDNVPLLRHSGADTVVTSASAAGRLLGLGTTSPEVVETLEDLLSSGS
                     GIDLVAETVGAEEAGPIGEADRRCGGPVLMAYREGRSLRRGDSELTEVKPGDTVVHVH
                     CRGAR"
     gene            1082..1870
                     /locus_tag="VHF88_02260"
     CDS             1082..1870
                     /locus_tag="VHF88_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2160621.1"
                     /translation="MSTSPVRLERDSDNGLAELVLASPPLNLFDRPLIDGLIDAIAAV
                     AAEPPRALLVRAEGKVVSGGVNVELFDGLTPEAAGELWSELLGKIVEPIEQLPAPVVF
                     AAHGLTLTAAFEIALACDLIVAARSAKFGLVETVVGLTPSMGGTQRLAERAGSGRARE
                     LVMTGELFGVEQLERWDVVSRIFEDGEFEQRSRELALKLAAGPTRAHAMTKHVVRRYV
                     AGGVEAADEAIRRDAGALFASEDLKGAVKSFLERGPGHAEFHNR"
     gene            complement(1883..2467)
                     /locus_tag="VHF88_02265"
     CDS             complement(1883..2467)
                     /locus_tag="VHF88_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2160622.1"
                     /translation="MSPREATEDDRTATRVARGDVLAWAAMDLADVPDLDPEAFDVHR
                     MIDAAAQQFAAPAEEKGIALEVKRAASVPRLALGEVEWLREVLCGLMDNAVRFTDSGE
                     VVASITADRTGGRRVMFHAEVSDTGCGMSADVLARLFGPRHGGRFTPLGGLDGAGTLG
                     VAQRLVELMDGRLGCSSALGLGTTTWFTVPLDLP"
     gene            2566..>3230
                     /locus_tag="VHF88_02270"
     CDS             2566..>3230
                     /locus_tag="VHF88_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2160623.1"
                     /translation="MPTSLVTGGAGFLGSNLCDHLLERGHRVICVDNLETGSLRNIKH
                     ICRDDFEFKMLDITEHYEIDEPIDFVFHMASPASPIDYARLPLHTLKVGAYGTHNSLG
                     LAKAKRARFLLASTSEVYGDPLVHPQPESYWGNVNPIGPRGVYDEAKRYAEAMTMAYL
                     RQQGVDTCIVRIFNTYGPRMRVNDGRAVPTFLDQALRDQPLTVFGDGSQTRSFCYVDD
                     LIRG"
BASE COUNT          498 a         1151 c         1116 g          465 t
ORIGIN      
        1 cgggcgccgc ggcagtgcac gtggacgacc gtatcgcccg gcttgacctc ggtgagctcg
       61 ctgtcgccgc ggcgcagcga ccggccctcg cggtaggcca tcagcaccgg tccgccgcag
      121 cggcggtcgg cctcgccgat cggcccggcc tcctcggcac cgacggtctc cgcgacgagg
      181 tcgatcccgc tcccggacga cagcaggtcc tccagagtct cgaccacctc tggcgaggtg
      241 gtcccgaggc cgagcagccg cccggcggcg ctcgccgacg tcacgaccgt atcggcgccg
      301 gagtggcgca gcagcgggac gttgtcctcc tcgcgcgccg cggcggtgat cgtcgcgttc
      361 ggcgccagct cgcgtgccgt cagggtcacc aacacggagg tgtcgtcgcg gtcagtcgcg
      421 acgatcaggg cagcggcgtc cggcgcggcc gcctcggtca acacgtcgcg ccgcgccgcg
      481 ctgccgagca cgccggtcac gccctctcgc gtcgccagct cgatcgcctc caggcgctcg
      541 tcgatcacga cgatctggtc gaggctggtg cccgtgccga tcaacgagcg caacgcgctg
      601 cgccccttgg tcccaaaacc gcagatgact acgtggtcgt gcaacttcct cctccagatc
      661 cgtcgtcgca gattggcgcg cgtggcggac gccaggaact cgagcgtcgt gccgaccagc
      721 aggatcagga agagcacccg cagtggcgtt acgaggaacg tcgtcagcag gcgggcggtg
      781 tcggacaccg ggacgatgtc gccgtagccg gtagtcgtga tcgagacggt cgcgtagtag
      841 atgcagtcga gcagcgacac cgagccgtcc gcgtcgcggt agccgccacg gtcggcgaag
      901 gcgatcgccg ttacgagccc gaccaggacg agtgcgatca gcaggcgaaa cccgagcgcc
      961 cgcgcgggcg agccggtgcg cgaggggaag tggatgttct ccgcggagat cgccatcccg
     1021 cgcgacgcta cgcacggcgc cggacggggt aaagccgccc gacgccgata cgctgcgcgg
     1081 catgtcgacg agccccgtcc gcctcgagcg cgacagcgac aatggcctcg ccgagctggt
     1141 cctcgccagc ccgccgctga acctcttcga ccggccgctg atcgacggcc tgatcgatgc
     1201 gatcgccgcc gtcgccgccg agccgccgcg cgcgctgctc gtccgagccg agggcaaggt
     1261 cgtgtcgggc ggtgtgaacg tcgagctctt cgacggtctg acgccggagg ccgcgggcga
     1321 gctctggagc gagctgctcg gcaagatcgt cgagccgatc gagcagctcc ccgcgccagt
     1381 cgtcttcgcc gcgcacggcc tgacgctgac ggccgcgttc gagatcgcgc tggcgtgcga
     1441 cttgatcgtc gccgcgcggt cagcgaagtt cggcctggtc gagacggtcg tcgggctgac
     1501 gccctcgatg ggcggaaccc aacgccttgc cgagcgggct ggcagcggcc gcgcccgcga
     1561 gctcgtgatg accggggagc tgttcggagt cgagcagcta gagcgctggg acgtcgtcag
     1621 ccggatcttc gaggatggtg agttcgagca gcggtcgagg gagctggcgc tgaagctcgc
     1681 ggccggcccg acacgtgcgc acgctatgac caagcacgtc gtgaggcgct acgtcgccgg
     1741 cggcgtcgag gcggccgacg aggcgattcg ccgtgacgcc ggcgcgctgt tcgccagcga
     1801 ggatctcaag ggcgccgtca agagcttcct cgagcgcggt cccggccacg ccgagttcca
     1861 caaccgctag agcgggaggg agtcagggca ggtcgagcgg gacggtgaac caggtcgtcg
     1921 taccgaggcc gagcgcgctg ctgcagccga gccggccgtc catcagctcg accagccgct
     1981 gggcgacgcc caacgtccct gccccatcca ggccgccgag cggcgtgaag cgaccgccgt
     2041 ggcgtgggcc gaacagccgc gccagcacgt cggcggacat cccgcagccg gtgtcggaga
     2101 cctcggcgtg gaacatgacc cgccgaccgc cggtgcgatc ggcggtgatg gatgcgacga
     2161 cctcgccgct gtcggtgaag cggacggcgt tgtccatcag tccgcagagc acctcgcgca
     2221 gccactccac ttccccgagt gcgaggcgcg gaacggaggc ggcccgcttg acctcgagcg
     2281 cgatgccctt ctcctcggcc ggggccgcga actgctgcgc cgctgcgtcg atcatccgat
     2341 ggacgtcgaa cgcctcgggg tcgagatcgg gcacgtcggc gaggtccatc gccgcccagg
     2401 caagcacgtc tccccgggcg acgcgggtag ctgtcctgtc gtcctcagtg gcctcgcgtg
     2461 gactcacctg gccctcctcc tcgagccgac cgtaccatgg cgaaggtgtc cccgtgaagg
     2521 gcttcaccac tcggattccc ctgcgctgga tgtagcctca tggccatgcc aacctctctt
     2581 gtcaccggcg gcgccggctt tctcgggtcg aacctctgcg atcacctgct cgagcgcgga
     2641 caccgggtca tctgcgtcga caacctcgag accggcagcc tacgcaacat caagcatata
     2701 tgcagggacg acttcgagtt caagatgctc gacatcaccg agcactacga gatcgacgag
     2761 ccgatcgact tcgtcttcca catggcttcg ccggcgagcc cgatcgacta tgcgcggctg
     2821 ccgctgcaca cattgaaggt gggcgcctac gggacgcaca actcgctcgg cctcgcgaag
     2881 gcgaagcgcg cgcgcttcct gctcgcctcg acctcggagg tctacggcga cccgctggtc
     2941 cacccgcagc cggagagcta ctggggcaac gtcaacccga tcggtccgcg cggcgtctac
     3001 gacgaggcca agcgctatgc cgaggcgatg acgatggcct acctgcgcca gcagggcgtc
     3061 gacacctgca tcgtgcggat cttcaacacc tacggcccgc ggatgcgggt caacgacggc
     3121 cgcgccgtcc cgacgttcct tgaccaggcg ctgcgcgacc aaccgctgac ggtcttcggc
     3181 gacggcagcc agacgcgcag cttctgctac gtcgacgacc tgattcgcgg
//
LOCUS       DATABV010000056         5586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2266501, whole genome shotgun sequence.
ACCESSION   DATABV010000056 DATABV010000000
VERSION     DATABV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266501"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VHF88_02275"
     CDS             <1..341
                     /locus_tag="VHF88_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160624.1"
                     /translation="DAAPAARVRAGVAALLDFCAAEPDRARACLVEALSAGPAARERR
                     SETNRRLADLFAQPLRELRPNARIAEVSAIALVGGFHELAYDAVDRRAPARLPSVETV
                     VAIARLEPLA"
     gene            380..1129
                     /locus_tag="VHF88_02280"
     CDS             380..1129
                     /locus_tag="VHF88_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2160625.1"
                     /translation="MAADERHTGDDEAGAAPAAEDATPARPRRKLPPRRPDQLPRGRH
                     GLSPDEVATSQRNRILDAMKALAGERGYHESSIADVIARAGVTRKAFYRLFDDKEQCF
                     IQAYERDLARLLTLTLEAFETQDAWADRVRAALSALLHALARDPAAARLCFIEVLAAG
                     PRAVAARNEAMRGFTILFDTGRLEDDRARPPALALNMVGGMSEIVHREIAAGRTANLP
                     AVLPDLMYTAVLPILGPEAAERELGRGYRGA"
     gene            complement(1173..1592)
                     /locus_tag="VHF88_02285"
     CDS             complement(1173..1592)
                     /locus_tag="VHF88_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2160626.1"
                     /translation="MELADTSAWWVADRDAVGPLREDFAQRLVTGSIATCDMVRFELL
                     HSARKGREMAETADELAAVPDCPIGGEQWQRALWVYQRLADQGGAHHRSVGYPDLLIA
                     AAAEAAGATVLHYDEDYERIAEITGQPTRWIAPRGSL"
     gene            complement(1596..1814)
                     /locus_tag="VHF88_02290"
     CDS             complement(1596..1814)
                     /locus_tag="VHF88_02290"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX2160627.1"
                     /translation="MKRTTINLDVDLAAEASDLLGTSRMTDTVHAAMRDVIRRRKLES
                     LSRRRFPDLTLESIEEDRRPRSFEHLRD"
     gene            1907..3343
                     /locus_tag="VHF88_02295"
     CDS             1907..3343
                     /locus_tag="VHF88_02295"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX2160628.1"
                     /translation="MDRLFAEDEGAATRGRTAVPPGAQPLAARMRPVELGEFVGQEHL
                     LEQGSALRKALEDGHPHSMILYGPPGTGKTTLARLIAVTANAAFEEISAVNAGRKEVR
                     EVLDRAAERRRGGNGEGTILFLDEIHRFNKAQQDVLLPAVEEGLVWLVGATTENPYFE
                     VNSALLSRCRVYELRALEESDVLALLRRALNDPERGIAQPPEVDEDALEFLAARAGGD
                     ARTALAALELAAETAAAEAGATTPDVGSASPGHANATSDSAGDNDRGHVTLARAEDAL
                     QRRAVLYDKDGDRHYDLISAWIKATRGSDPDASLLYMAAMLEGGEDVRFIARRMVILA
                     SEDVGNADPQALLIATAAAQAVDRVGLPECHFALAQATVYLALAPKSNAAGAALGHAR
                     AWVRENGTPVPPDYLRDAHYPGAKKLGRGEGYDYPHAHPTGTSPQELMPDNAVGRRFL
                     ELSAFGHERELAERLDGIRRARRRDPDT"
     gene            3433..5019
                     /locus_tag="VHF88_02300"
     CDS             3433..5019
                     /locus_tag="VHF88_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2160629.1"
                     /translation="MEAKSPPAQGQLETFSPIDGRRLGAVPTVTPEQVQSVVDDVAEV
                     QPFWAALPVEDRARYMRRAAQVIIDSLDVLSELLTREQGKPRNESYVMELLPTIDALN
                     WVADAGPEILADEKVKLPIWVKQKQARFAYEPLGVVGVIAPWNYPWSIPMGEVAIALM
                     CGNGVVLKPASLTPLIGQKIQEVFERAGLPEGLVRTVHGGGAVGNALVESSAAKIFFT
                     GSVEVGRAVGVKCAERMKGSVLELGGKDPMIVLSDANMANAVSGALWGGFANAGQTCS
                     GIERVYVMRDVAERFTDELVRGAQALHVGDPMDWRTEIGPMVSREQHDLVVELVDDAV
                     AAGATLRCGGPTPPPEGGTGNWYAPAVLTDVTHDMRIMREEIFGPVVPVVPVDSEDEA
                     IALANDSEFGLGASVWTTDRARGDRIARRIQSGMVWVNDHMYSHGTCSCAWGGVKDSG
                     VGRSHSKFGFYECVNVKQLSWEPGLTRNFWWHPYDRSLGRSMHEAATLLYGRDSDKAA
                     ALRRGARSFARLGAKIVKRS"
     gene            5077..>5586
                     /locus_tag="VHF88_02305"
     CDS             5077..>5586
                     /locus_tag="VHF88_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2160630.1"
                     /translation="MTTTASQDRLWALDSELEALKASFEELLASFGDAFGAVVARPDI
                     DGSIKVRLADVANRLRNLKPELEALDFDVEQSAALFSAIVDVDRAVQAEHEDFDRFEQ
                     MLLGIERIRQVIRDAIDESVGGVDADRRRQLEALQEGLPGVKQIQIAELLGVDSRTVR
                     RWGDRPGRAD"
BASE COUNT          809 a         2002 c         2005 g          770 t
ORIGIN      
        1 ccgacgccgc ccccgccgcc cgcgtccgcg ccggcgtcgc cgcgctgctc gacttctgcg
       61 ccgccgaacc cgaccgcgcc cgcgcgtgcc tcgtcgaggc gctcagcgcc gggccggccg
      121 cgcgcgagcg gcgctcggag accaaccgcc ggctggccga cctgttcgcc cagccgctgc
      181 gcgagctgcg gcccaacgcc cgcatcgccg aggtgtcggc aatcgcgctc gtcggcggct
      241 tccacgagct cgcctacgac gccgtcgacc ggcgcgcgcc cgcccggctg ccatcggtcg
      301 agacggtcgt cgcgatcgcc cggctcgagc cgctcgccta gcggtcccgg tcggggtatc
      361 gtgcggtttg tgatgcgcga tggccgctga cgagcgccac accggcgacg acgaggccgg
      421 cgcggcgccg gcggccgagg acgcgacacc cgcccgtccc cgccgcaagc tgcccccgcg
      481 ccggcccgac cagctaccgc gcgggcgcca cgggctctcg cccgacgagg tcgccacctc
      541 gcagcgcaac cggatcctcg acgcgatgaa ggcgctagcc ggcgagcgtg gctaccacga
      601 gtcctcgatc gccgacgtga tcgcgcgcgc cggcgtcacg cgcaaggcct tctaccggct
      661 gttcgacgac aaggagcagt gcttcatcca ggcctacgag cgcgacctcg cgcgcctgct
      721 gacgctcacc ctggaggcgt tcgagaccca ggacgcctgg gccgaccgcg tccgcgccgc
      781 actcagcgca ctcctccacg cgctcgcgcg cgaccccgcc gccgcccggc tctgcttcat
      841 cgaggtgctc gccgccggtc cgcgcgcggt cgccgcgcgc aacgaggcga tgcgcggctt
      901 cacgatcctc ttcgacaccg gccgcctcga ggacgaccgc gcccgcccgc cggcgctcgc
      961 gctgaacatg gtcgggggga tgagcgagat cgtccaccgc gagatcgccg ccggccgcac
     1021 cgccaacctt ccggctgtcc tgcccgacct gatgtacacc gcggtcctgc cgatcctcgg
     1081 gccggaggcc gcggagcggg agctcgggcg cggctaccgc ggcgcgtagc gctgaaggac
     1141 tgccacccgc gcatggaccc ggcgctcctc cgctagagcg acccccgcgg agctatccag
     1201 cgcgtgggct ggcccgtgat ctcggcgatg cgctcgtagt cctcgtcgta gtggagcact
     1261 gtcgcgcccg ccgcctccgc ggcagcggcg atcaggaggt cggggtatcc gaccgatcgg
     1321 tgatgagcgc caccctggtc ggccagacgc tgatacaccc aaagcgcgcg ctgccactgc
     1381 tccccgccga tcgggcagtc gggcaccgcg gcaagctcgt ccgcggtctc agccatctcc
     1441 cggcccttgc gcgcgctatg caggagctcg aagcggacca tgtcgcaggt cgcgatcgag
     1501 ccggtgacga gccgctgcgc gaagtcctca cgcaagggtc ccacggcgtc ccggtctgcg
     1561 acccaccatg cggaggtgtc ggcgagctcc accgctcagt cgcgtagatg ctcgaaagag
     1621 cgcgggcgac ggtcctcctc gatcgactcc agcgtcagat cgggaaagcg ccggcgcgac
     1681 aggctctcca gcttccggcg tctgatcacg tcgcgcatcg ccgcatggac cgtgtcggtc
     1741 atccgcgagg tgccgagcaa gtcggaagcc tcggcggcga ggtcgacgtc gaggttgatc
     1801 gtcgtgcgtt tcactcgagg catcgtatgg atgggatcgt gacagatgta tggctcatag
     1861 tgacagcggc gtatggatgt tccgcccgtg ctgcgacaat cgggctgtgg accggctgtt
     1921 cgcagaggac gagggcgcgg cgacgcgggg gcggactgcg gtgccgccgg gggcgcagcc
     1981 gcttgcggct cggatgcggc cggtcgagct gggcgagttc gtcggccagg agcacctgct
     2041 cgagcagggg tcggcgctgc ggaaggccct cgaggacggc cacccgcact cgatgattct
     2101 ctacgggcct cccgggaccg ggaagacgac gttggcgcgg ctgatcgcgg tgacggcgaa
     2161 cgcggcgttc gaggagatct ccgcggtcaa cgcggggcgc aaggaggtgc gtgaggtgct
     2221 cgaccgcgcc gcggagcggc ggcgcggcgg caacggcgag gggacgatcc tcttcctcga
     2281 cgagatccac cgcttcaaca aggcccagca ggacgtgctg ctgccggcgg tcgaggaggg
     2341 gctggtgtgg ctggtcgggg cgacgaccga gaacccgtac ttcgaggtca actcggcgct
     2401 gctgtcgcgc tgtcgcgtgt acgagctgcg cgcgctcgag gagagcgatg tgctggcgct
     2461 gctgcggcgg gcgctgaacg acccggagcg cgggatcgcc cagccgccgg aggtagacga
     2521 ggacgcattg gagttcctcg cggcgcgggc tggcggcgac gcgcgaaccg cgctcgccgc
     2581 gctcgagctc gcagccgaga cggcggccgc cgaggccggc gcgaccacgc cagatgtcgg
     2641 atctgcatcg ccaggacacg cgaatgcgac atctgactcc gcgggcgaca atgaccgcgg
     2701 tcacgtcacg ctcgcccgcg ccgaggacgc gctgcagcgc cgcgccgtcc tctacgacaa
     2761 ggacggcgac cgccactacg acctgatctc ggcctggatc aaggcgacgc gcggctcgga
     2821 ccccgacgcg tcgctgctct acatggcggc gatgctcgag ggcggcgagg acgtccgctt
     2881 catcgcccgc cgcatggtca tcctcgcgag cgaggacgtc ggcaacgccg acccgcaggc
     2941 gctgctgatc gccaccgcgg ccgcccaggc ggtcgaccgc gtcggcctgc ccgagtgcca
     3001 cttcgcgctc gcccaggcga cggtctacct ggcgctggcg ccgaagtcca acgccgccgg
     3061 cgcggcgctc ggccacgcgc gcgcgtgggt gcgcgagaac gggacgccgg tcccgcccga
     3121 ctacctgcgc gacgcccact acccgggcgc gaagaagctg gggcgcggcg agggctacga
     3181 ctacccgcac gcccacccga ccggcacctc gcctcaggag ctgatgcccg acaacgccgt
     3241 cggccgccgc ttcctcgagc tgtcggcctt cggccacgag cgcgagctcg ccgagcggct
     3301 tgatggcatc cgccgcgctc gccgccgcga tcccgatacc tgagcattgt gaggtcgact
     3361 ttcccgacat atggcgtcgg gaaggtcgac ctcgagctcc cgaggccggt cgggggcgtc
     3421 acaatgagcg ccatggaggc caagtcaccg cccgcgcagg gccagctcga gacgttctcg
     3481 ccaatcgacg gtcgccgcct cggcgccgtc ccgaccgtca cgccggagca ggtgcagtcc
     3541 gtcgtcgacg acgtggccga ggtgcagccg ttctgggccg cgttgccggt cgaggaccgc
     3601 gcgcgctaca tgcgccgcgc ggcgcaggtg atcatcgact cgctcgacgt gctgagcgag
     3661 ctgctcacgc gcgagcaggg caagccgcgc aacgagtcgt acgtgatgga gctgctgccg
     3721 acgatcgacg cgctcaactg ggtcgccgac gcgggcccgg agatcctcgc cgacgagaag
     3781 gtcaagctgc cgatttgggt caagcagaag caggcgcggt tcgcctacga gccgctcggc
     3841 gtcgtcggcg tcatcgcgcc ctggaactac ccgtggtcga tcccgatggg cgaggtcgcg
     3901 atcgcgctga tgtgcggcaa cggcgtggtc ctgaagccgg cgtcgctgac gccgctgatc
     3961 ggccagaaga tccaggaggt cttcgagcgg gccgggctac cggaggggct ggtgcggacg
     4021 gtccacggcg gcggcgcggt cggcaacgcg ctggtcgagt cgagcgcggc gaagatcttc
     4081 ttcacggggt cggtggaggt ggggcgcgcc gtgggcgtca agtgcgccga gcggatgaag
     4141 ggctcggtgc tcgagctcgg cggcaaggac ccgatgatcg tgctgtcgga cgccaacatg
     4201 gcgaacgccg tgtcgggcgc gctctggggc gggttcgcga acgcggggca gacgtgcagc
     4261 gggatcgagc gcgtctacgt gatgcgcgac gtcgccgagc gcttcaccga cgagctggtg
     4321 cgcggggcgc aggcgctgca cgtcggcgac ccgatggact ggcgcaccga gattgggccg
     4381 atggtgtcgc gcgagcagca cgacctcgtg gtcgagctgg tcgacgacgc cgtcgcggcg
     4441 ggcgccacgc tgcgctgcgg cgggccgacg ccgccgcccg agggcgggac cggcaactgg
     4501 tacgcgcccg ccgtcctgac cgacgtcacc cacgacatgc ggatcatgcg cgaggagatc
     4561 ttcggcccgg tggtcccggt ggtcccggtc gactccgagg acgaggcgat cgcgctggcc
     4621 aacgactccg agttcggcct cggcgcctcg gtctggacta ccgaccgcgc gcgcggcgac
     4681 cgtatcgccc ggaggatcca gtccggcatg gtctgggtca acgaccacat gtactcgcac
     4741 gggacctgct cctgcgcctg gggcggcgtc aaggactccg gcgtcggccg ctcgcactcg
     4801 aagttcggct tctacgagtg cgtcaacgtc aagcagctct cctgggagcc cggcctgacg
     4861 cgcaacttct ggtggcaccc ctacgaccgc tcgctcggcc gctcgatgca tgaggccgcg
     4921 acgctcctct acggccgcga ctccgacaag gcggcggcgc tgcgccgcgg cgcacgctcg
     4981 ttcgcgcggc tgggcgcgaa gatcgtcaag cgcagctgag tcgaggcgct gctaggctgt
     5041 cctcaacgtg tcctatcgat cggacaggaa gaggacatga ctaccaccgc cagccaggac
     5101 cgcctctggg ccctcgactc ggagctagaa gccctgaagg cctcgttcga ggagctactc
     5161 gcctcgttcg gcgacgcctt cggcgcggtc gtcgcgcggc ccgacatcga cggatcgatc
     5221 aaggtgcggc tagccgacgt ggctaatcgg ttacgcaacc tcaagcccga gctcgaggca
     5281 ctcgacttcg acgtcgagca atcggccgcg ctgttctcag cgatcgtcga cgtcgaccgc
     5341 gccgtacaag ccgagcacga ggacttcgac cgctttgagc aaatgctgct cgggatcgag
     5401 cgcatccgcc aggtgatccg cgacgcgatc gacgagtccg tcggcggcgt cgatgccgac
     5461 cgtcgccgcc agctggaagc gcttcaggag ggcctaccag gggtcaagca gatccagatc
     5521 gccgagcttc tcggcgttga ctcccgaaca gtgcggcgct ggggcgatcg gcccggccgg
     5581 gccgat
//
LOCUS       DATABV010000057         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2026062, whole genome shotgun sequence.
ACCESSION   DATABV010000057 DATABV010000000
VERSION     DATABV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026062"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2165)
                     /locus_tag="VHF88_02310"
     CDS             complement(<1..2165)
                     /locus_tag="VHF88_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX2160631.1"
                     /translation="MADRDYLQAVRERVVVFDGGMGATLEQFDLTQEDYGGLEGKCHE
                     ALVLNRPDVIEGVHRSMAEAGAEVVETDTFQASRLKLGEWGLADHTVEINQRAAEIAR
                     KAVGEDRFVAGSIGPTGFLPASDDPTLGDIRFRDLVEVFQEQAEGLIQGGADLLIIET
                     AQDILEVKAAIFGAREAFKTTGRRLPIQCSVSLLPNGGKMLLGTDVQAVLTTLTALDV
                     QVIGLNCSTGPEDMRDAIRFLGENSPLPVHCIPNAGLPLQGPDGETIFPEEPESLSAT
                     LQEFVERYGVGIVGGCCGTTPDHIRAIVEKVGGTPVGPRPAPGPPFVSSMMTATPLVQ
                     EPRPTLVGERVNSQGSRKAKELLLADDYDGLVQIAEDQVEGGAHVLDLCVALTERTED
                     EAEQMRKVAERVSLTQPAPIQVDSTEPDVIEEALEQIPGRAIVNSVNLEAGRDKLDRV
                     VPLAIAHGAAVIALTIDEVGMAKTRERKLEIAKRIKQLACDEHGLDPELLIFDLLTFT
                     LTTGDEEWRPSAVETIEGIRLLKQELPEVKTSLGVSNVSFGVSPHARAALNSVFLHHC
                     VEAGLDLAMVNPNHITPYSEIPDEERELVDDLVLNRREDALERFIGHYESKGAEAEAE
                     VADPTAGMEPEQALHWHILRRKRDGVEDQIDKSVEKIGAVPTLNQVLLPAMKEVGDKF
                     GAGELILPFVLQSAEVMKRAVAQLENYLERMEGHTKGKVV"
     gene            complement(2228..>2602)
                     /locus_tag="VHF88_02315"
     CDS             complement(2228..>2602)
                     /locus_tag="VHF88_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2160632.1"
                     /translation="YRPEAGPGAVVAALALVVVFAFGLSWAFTTLGLVMRAPNAVMNT
                     GFMVLFPITFISNAFVEEETLPSALQTVVDVNPISHLITAARGLMDGNPDGGEILLVL
                     AVAAALTLLFAPITSRLYRRAA"
BASE COUNT          366 a          877 c          938 g          421 t
ORIGIN      
        1 acgaccttgc ccttggtgtg gccctccatc cgctcgaggt agttctcgag ctgggcgacg
       61 gcgcgcttca tcacctccgc cgactgcagt acgaagggca ggatcagctc gccggcgccg
      121 aacttgtctc cgacctcctt catcgccggc aggagcacct ggttgagcgt cgggacggcg
      181 ccgatcttct cgaccgactt gtcgatctgg tcctcgacgc cgtcgcgctt gcggcggagg
      241 atgtgccagt gcagcgcctg ctcgggctcc atgccggcgg tcgggtcggc gacctcggcc
      301 tcggcctccg cgcccttcga ctcgtagtga ccgatgaagc gctcgagcgc gtcctcgcgg
      361 cggttgagca cgaggtcgtc gacgagctcg cgctcctcgt cggggatctc gctgtagggc
      421 gtgatgtggt tggggttgac catcgcgagg tcgagcccgg cctcgacgca gtggtgcagg
      481 aagaccgagt tgagcgccgc gcgggcgtgc ggggagacgc cgaaggagac gttcgagacg
      541 ccgagcgagg tcttgacctc gggcagctcc tgcttgagca gccggatccc ctcgatcgtc
      601 tcgacggccg acggccgcca ctcctcgtcg ccggtcgtga gcgtgaaggt gagcaggtcg
      661 aagatcagca gctcggggtc gaggccgtgc tcgtcgcagg cgagctgctt gatccgcttg
      721 gcgatctcga gcttgcgctc gcgcgtcttg gccatcccga cctcgtcgat cgtcagcgcg
      781 atcacggcgg cgccgtgggc gatcgcgagc gggactacgc ggtcgagctt gtcgcggccg
      841 gcctcgaggt ttaccgagtt gacgatcgcg cggccgggga tctgctcgag cgcctcctcg
      901 atcacgtccg gctcggtcga gtcgacctgg atcggcgcgg gctgggtcag cgatacgcgc
      961 tcggcgacct tgcgcatctg ctcggcctcg tcctcggtgc gctcggtcag ggcgacgcag
     1021 aggtcgagca cgtgggcgcc gccctcgacc tggtcctcgg cgatctggac gaggccgtcg
     1081 tagtcgtcgg cgagcagcag ctccttcgcc ttgcgcgagc cctgcgagtt gacgcgctcg
     1141 ccgacgagcg tcgggcgcgg ctcctggacg agcggcgtcg cggtcatcat gctcgagacg
     1201 aacggcgggc cgggcgcggg gcgcgggccg acgggcgtgc cccccacctt ctcgacgatc
     1261 gcgcggatgt gatcgggcgt cgtgccgcag cagccgccga cgatgccgac gccatagcgc
     1321 tcgacgaact cctgcagcgt ggcggacagc gactccggct cctcggggaa gatcgtctcg
     1381 ccgtctgggc cctggagcgg caggccggcg ttggggatgc agtgcaccgg cagcgggctg
     1441 ttctcgccga ggaagcggat cgcgtcgcgc atgtcctcgg gaccggtcga gcagttgagc
     1501 ccgatcacct ggacgtcgag cgcggtcagc gtcgtcagca ccgcctgtac gtcggtcccg
     1561 agcagcatct tgccgccgtt gggcagcagc gagaccgagc actggatcgg caggcgacgg
     1621 ccggtcgtct tgaaggcctc gcgggcgccg aagatcgccg ccttgacctc gaggatgtcc
     1681 tgcgcggtct cgatgatcag taggtcggcc ccaccctgga tcaggccctc ggcctgctcc
     1741 tggaagacct cgacgaggtc gcggaagcgg atgtccccta gcgtcgggtc gtcggaggcg
     1801 ggcaggaagc cggttggtcc gatcgagccg gcgacgaagc ggtcctcgcc gaccgccttg
     1861 cgcgcgatct cggcggcgcg ctggttgatc tcgacggtgt ggtcggcgag accccactcg
     1921 ccgagcttga gccggctggc ctggaaggtg tcggtctcga cgacctcagc tcccgcctcg
     1981 gccatcgagc ggtggacgcc ctcgatcacg tcgggacggt tgagcacgag cgcctcgtgg
     2041 cacttgccct ccagcccgcc gtagtcctcc tgggtgaggt cgaactgctc gagcgtcgcg
     2101 cccatgccgc cgtcgaagac gacgacgcgc tcgcggacgg cttggaggta gtcgcgatcg
     2161 gccatggccg gccaggctat cgaaccggcg cgaaccttga cacgatcgtg tgaaggttca
     2221 ctggtcgtca ggcagcccgg cggtacaggc gcgaggtgat cggcgcgaac aggagcgtca
     2281 gcgcggccgc gacggccagc acgagcagga tctcgccgcc gtcggggttg ccgtccatca
     2341 gcccgcgcgc cgccgtgatc aggtggctga tcggattgac gtcgacgacc gtctgcagcg
     2401 cgctcggcag cgtctcctcc tccacgaacg cgttgctgat gaaggtgatc gggaagagaa
     2461 ccatgaagcc ggtgttcatc accgcgttgg gcgcgcgcat caccaggccg agcgtcgtga
     2521 acgcccacga gagaccgaac gcgaagacga cgaccagcgc gagggccgcc accaccgcac
     2581 ccggccccgc ctcgggccgg ta
//
LOCUS       DATABV010000058         9830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_740711, whole genome shotgun sequence.
ACCESSION   DATABV010000058 DATABV010000000
VERSION     DATABV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740711"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHF88_02320"
     CDS             <1..201
                     /locus_tag="VHF88_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160633.1"
                     /translation="RRTAPRPTDPPLELRAATAARDRLLADGGPVDLAGRAGSIVVAA
                     ATSLRAVLTPRGPEPVLGAPPG"
     gene            214..1857
                     /locus_tag="VHF88_02325"
     CDS             214..1857
                     /locus_tag="VHF88_02325"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX2160634.1"
                     /translation="MSERRASDLFVECLEAEGVKHVFGIPGEETLDLNESLSRSSIDF
                     VPVRHEQGGAYMADVYGRLTGHAGVCLGTLGPGALNLVTAVADAYLDRAPLVALTGQG
                     DLERMHKESHQYIDLLRVMRPITKWNARLSDPAIIPEVVRKAFKVAESEKPGATHIEL
                     PEDVMAAPVDGAPLPRHKAASPEPQARELLRAADLIRGAVRPVALAGNGVIRGGAAAA
                     LREFVRATGIPVAETFMGKGLLPYDNPKSLGSVGLQAGDYRMAGFDDADVVLAIGYDL
                     VEHSPEHWNPKRDKRIVCIDSVPAETDANFIPEVELVGDLYAILARLGEECRHVPHQG
                     GSTQLRDVVLGRFEAARDDDAFPVQPPRALWEIRQAPGREDILISDVGLHKLWIGRMF
                     PAHEPNTVLIANGLAGMGFAVPAAVAAKLVHPERNVVTVNGDGGFLMNMQELETATRL
                     KTPFVNVVWENRQYGSIVWKQDKKFGSHFGVDFTNPDFVKLAESFGLPAWRCDSADDF
                     GARLRHALTLDLPSLIVLPIDYSLDVAISEELGAEIVST"
     gene            1964..3475
                     /locus_tag="VHF88_02330"
     CDS             1964..3475
                     /locus_tag="VHF88_02330"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2160635.1"
                     /translation="MAIATQEQSVVEAVPKGLYVGGEWREASGGATLAVEDPATGESL
                     CEVADATPEDARAALDAACAAQAEWAAHPPRERGEILRRAYEEMTARVDELALLMTLE
                     MGKTVAESKAEIAYASEFLRWNAEEAVRIDGRYATAPNGAGRLLTMKQPVGPCLLITP
                     WNFPTAMGTRKIGPAVAAGCTMVVKPAKLTPLSMLALAQILENAGLPAGVLNVVTASS
                     ASKTTGPLIEDPRLRKLSFTGSTEVGRKLIEQSAPNVLKLSMELGGNAPFLVFEDADL
                     DAAVEGAMVAKLRNIGEACTAANRFHVAAAVADEFADRLAERMGGLKVGRGTEEGVDI
                     GPLVDEDTRDKVTELVADAVDKGADALVGGEDARGGSANGAGDLGRLDGAGHFYAPTV
                     LKGVTPDARVLSEEIFGPVAPVIGFDSEDDALAAANDTEFGLVAYVYTRDFKRALRVC
                     EALETGMVGLNQGMVSNPAAPFGGVKQSGFGREGGYEGIEEYLETKYVAFDVA"
     gene            3552..4502
                     /locus_tag="VHF88_02335"
     CDS             3552..4502
                     /locus_tag="VHF88_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160636.1"
                     /translation="MEPRYSMAAYAAVVGAVLVLLALSGEHSGAAWAAAGLLAIASAL
                     DVTYVAQRRRLAREAPYRSKFDRMVRIRWVLAGIGSLALIAAAGALDLAPLDGQGRFV
                     DAVLLCSTVGALGVFVSSLVDWYWILPKVSGIVCPAPCEAPGGERWESPTGHWYFHRG
                     VATLLVSGMATGICGYMAATTDDQGEAAIWGVVALVAISTAVVKFNEQGIKALWYGFS
                     PPIHVGDTILAERHTEGPVERRRAYVVDVSLQGARYKFLIDGRYAGTRFGTKGDQAPI
                     PNDDLLGTPRAEPIAVCSESCTGINWYCRYNPSAHDKVRK"
     gene            complement(4522..5844)
                     /locus_tag="VHF88_02340"
     CDS             complement(4522..5844)
                     /locus_tag="VHF88_02340"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX2160637.1"
                     /translation="MAVEQQLWGSETSKAIDNFPVSGERVPTAVVRMLGQIKAAAARV
                     NGELGLLDGAIAERIAAAGDEIARGEHDDQFPIDVFQTGSGTSSNMNSNEVIANLAGD
                     GAHANDHVNMGQSSNDVFPSAVHLAAVSETHAKLLPALEKLAGALEVKADAWRDVVKA
                     RRTHMMDAVPVTLGQEFAGYAKQVSLGRDRVGDALPRASQIPLGGTATGTGLNTHPEF
                     AARVRGKLREATGIDVEGPADPFEAQANRDALVELSGALKVVAVSLTKIANDLVIMGS
                     GPRTGFGELALPELQKGSSIMPGKVNPVIPEVVLQVAAQVIGNDVAITVAGSQGNFEL
                     NVRVPMIARNLLESITILTAACTLLADKVIDGLEANEEALTRNAESTPAIATALNPHI
                     GYDKATEIVKEAVASRRTIREVALEKGVDEATLDEALDLRKMARGSGG"
     gene            6061..6582
                     /locus_tag="VHF88_02345"
     CDS             6061..6582
                     /locus_tag="VHF88_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160638.1"
                     /translation="MPLIHTDFERTATEPSEPPAKKRFCSRCGEPAVDPPAGSEPPVL
                     EQRVCGVCGMGILLRCNPDSLPGAGSAFLITSHALEVAAVSEAAEPIFAPEEKLLGAN
                     LMDLLTSTIGDRRLAKTVSRAALRVHEAVTVPARLAGPKADEIGTMAARISTCGPPRA
                     ALIAVEQTHFGRR"
     gene            6800..7720
                     /locus_tag="VHF88_02350"
     CDS             6800..7720
                     /locus_tag="VHF88_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2160639.1"
                     /translation="MSGIRTNAAAEILGVSPNTLRSWERRFNYPQPRRTAGGHRQYDL
                     TELEALRRALMETHNISSAIEVARQRGEGPSSPSRLLDAFDHFDESAADRVMEESLAV
                     RSVDRSVEEVLLPAVELAEGRDGREAEQELACRWATGWLHAARRVVPPPTRSQGVILF
                     DASNGLDLESLHVQALELAIRRAGFRSILLSFDLPPERVARAMRAVEPTAFVFCGGEA
                     TLEVLGRLVYTVRQVGSAAPVFEYREAMPVTGDHSIPSLGSTPTEAADNLKQYVDTGK
                     IEHKVVQDEADADADAGKAPAKPVRLAAQR"
     gene            complement(7913..8851)
                     /locus_tag="VHF88_02355"
     CDS             complement(7913..8851)
                     /locus_tag="VHF88_02355"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX2160640.1"
                     /translation="MGTTSQRPPRDDARQLWDLARRQHGVVTRAQLLDLGLHRGAVAH
                     RLRSGRLHPVQRGVYAVGRPDLSQRGRWMAAVSSCGETAVLSHRSAAALHGIADPPAG
                     PIEVTVAPTTVRSRPGLITYRRLVDPDERTQADGVPVTTVERTLLDIAPMLSSKNLEA
                     AINTADKHDLVDPERLRSALERYAGRRGVRALRQLLDRHTFVLTASQLEREFLPIARS
                     AGLGQPKTGAWLNGFEVDFFWPDLGLVVETDGLRYHRTPAAQARDRLRDQAHAAAGLS
                     TLRFTHAQVRYEPDHVRETLAAVALRLGSTPRVRAR"
     gene            9239..9670
                     /locus_tag="VHF88_02360"
     CDS             9239..9670
                     /locus_tag="VHF88_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2160641.1"
                     /translation="MSLEVVDPLAHRALQALMRAETSVRRVLAAELEREGVSAAGFSA
                     LVVLTTAGGSLELRTLRRRLGWSKANATEVTATLEARGFIARRRSAGDRRAVLLDLTV
                     AGAEVVERLFPGHTERVRSTFSTLDEDEKRSLASLCRKLAA"
BASE COUNT         1436 a         3467 c         3504 g         1423 t
ORIGIN      
        1 cgccgcacgg caccccgacc caccgacccc ccgctcgagc tccgcgccgc caccgccgcg
       61 cgcgaccgcc tgctcgccga cggcggcccg gtcgacctcg ccggccgcgc cggctcgatc
      121 gtcgtcgccg cggcgaccag cctgcgcgcg gtcctcacgc cacgcggccc tgagccggtc
      181 ctcggagcgc cacccggcta gtctcgactc acggtgtcgg agcgacgcgc atcggacctc
      241 ttcgttgagt gcctcgaggc cgagggcgtc aagcacgtgt tcggcatccc gggcgaggag
      301 acgctcgacc tcaacgagtc gctgtcgagg tcgtcgatcg acttcgtgcc ggtccgccac
      361 gagcagggcg gggcctacat ggccgacgtc tacggccggt tgaccggcca cgccggggtc
      421 tgtctcggca cgctcgggcc gggggcgctg aacctcgtca ccgcggtcgc cgacgcctac
      481 ctcgaccgcg cgccgctggt cgcgctgacc ggccagggcg acctcgagcg gatgcacaag
      541 gagtcgcacc agtacatcga cctgctgcgc gtgatgcggc cgatcaccaa gtggaacgcg
      601 cgcctgtccg acccggcgat catccccgag gtcgtccgca aggcgttcaa ggtggccgag
      661 tcggagaagc ccggcgcgac gcacatcgag ctgcccgagg acgtgatggc ggcgccggtc
      721 gacggcgcgc cgctgccgcg tcacaaggcg gcgtcgcccg agccgcaggc gcgcgagctg
      781 ctgcgcgccg ccgacctgat ccgcggcgcg gtccgcccgg tcgcgctcgc gggcaacggc
      841 gtgatccgcg gtggcgcggc ggcggcgctg cgcgagttcg tgcgcgcgac cgggatcccg
      901 gtggccgaga cgttcatggg caaggggctg ctcccctacg acaacccgaa gtcgctcgga
      961 tcggtcgggc tgcaggcggg cgactaccgg atggcggggt tcgacgacgc cgacgtcgtg
     1021 ctggcgatcg gctacgacct cgtcgagcac tcgcccgagc actggaaccc gaagcgcgac
     1081 aagcggatcg tctgcatcga ctcggtcccg gcggagaccg acgccaactt catccccgag
     1141 gtcgagctgg tcggcgacct ctacgcgatc ctcgcgcggc tcggcgagga gtgccgccac
     1201 gtgccccacc agggcgggtc gacccagttg cgcgacgtgg tgctcgggcg cttcgaggcg
     1261 gcgcgcgacg acgacgcctt cccggtccag ccgccgcgcg cgctgtggga gatccgtcag
     1321 gcgcccgggc gcgaggacat cctgatctcg gacgtcggcc tacacaagct ctggatcggg
     1381 cggatgttcc cggcccacga gccgaacacg gtgctgatcg ccaacgggct ggcgggcatg
     1441 ggcttcgccg tgccggcggc ggtcgcggcg aagttggtgc accccgagcg caatgtcgta
     1501 accgtcaacg gcgacggcgg cttcctgatg aacatgcagg agctcgagac ggcgacccgg
     1561 ctgaagaccc cgttcgtcaa cgtcgtttgg gagaaccgcc agtacggctc gatcgtctgg
     1621 aagcaggaca agaagttcgg ctcccacttc ggcgtcgact tcaccaaccc cgacttcgtc
     1681 aagctcgccg agtccttcgg cctgcccgcc tggcgctgcg actccgccga cgacttcggc
     1741 gcccgcctcc gccacgccct cacgctcgac ctcccctcgc tgatcgtcct gccgatcgac
     1801 tactcgctcg acgtcgcgat ctccgaggag ctgggcgcgg agatcgtctc gacctagaag
     1861 tcgacgacgc aagacgtccg gtgggtggtc ccgcgcggaa gcgtcgcgtg agcggcgtct
     1921 agtctgggta gtgcccttga cgagctgagt gaaggagtcc tcgatggcga tcgcgaccca
     1981 ggagcagtcc gtagtcgagg cggtcccgaa gggcctgtac gtcgggggcg agtggcggga
     2041 ggcgagcggc ggcgcgacgc ttgcggtcga ggacccggca acgggcgagt cgctctgcga
     2101 ggtggcggac gcgacgcccg aggacgcgcg ggcggcgctc gatgcggcgt gcgcggcgca
     2161 ggcggagtgg gccgcgcatc cgccgcgcga gcgtggggag atcctgcggc gggcctacga
     2221 ggagatgacg gcgcgcgtgg acgagctggc gctgctgatg acgctcgaga tgggcaagac
     2281 ggtcgccgag tcgaaggcgg agatcgccta cgcgtcggag ttcctgcgct ggaacgccga
     2341 ggaggcggtg cggatcgacg ggcgctacgc gacggcgccg aacggcgcgg ggcggctgct
     2401 gacgatgaag cagccggtcg ggccctgtct gctgatcacc ccgtggaact tcccgacggc
     2461 gatgggaacc cgcaagatcg gcccggcggt cgcggccggc tgcacgatgg tcgtcaagcc
     2521 ggcgaagctg acgccgctgt cgatgctcgc gctcgcgcag atcctcgaga acgcggggct
     2581 gccggcgggg gtgctgaacg ttgtgaccgc ctcgtcggcg agcaagacga ccggtccgct
     2641 gatcgaggac ccgcgactgc gcaagctttc gttcaccggc tcgaccgagg tcggccgcaa
     2701 gctgatcgag cagtcggcgc cgaacgtgct gaagctgtcg atggagctcg gtggcaacgc
     2761 cccgttcctg gtcttcgagg acgccgacct cgacgccgcc gtcgaggggg cgatggtcgc
     2821 caagctgcgc aacatcggcg aggcctgcac cgccgccaac cgcttccacg tcgccgccgc
     2881 ggtcgccgac gagttcgccg accggctggc cgagcggatg ggcgggctga aggttgggcg
     2941 tgggaccgag gagggcgttg acatcggccc gctggtcgac gaggacacgc gtgacaaggt
     3001 gacggagctc gtcgccgacg cggtcgacaa gggcgccgac gcgctcgtcg gcggcgagga
     3061 cgcacgcggc ggcagcgcca acggagccgg ggatctcgga cggctcgacg gcgccgggca
     3121 cttctacgcg ccgaccgtcc tcaagggcgt cacgcccgac gcgcgtgtcc tctccgagga
     3181 gatcttcggg ccggtcgcgc ccgtgatcgg cttcgactca gaggacgacg cgctcgccgc
     3241 cgccaacgac accgagttcg gcctcgtcgc ctacgtctac acgcgcgact tcaagcgcgc
     3301 actgcgcgtc tgcgaggcgc tcgagacggg gatggtcggc ctcaaccagg ggatggtctc
     3361 caacccggcc gccccgttcg gcggcgtcaa gcagtccggc ttcggtcgcg agggcggcta
     3421 cgagggcatc gaggagtacc tcgagaccaa gtacgtcgcc ttcgacgtgg cgtagcctca
     3481 cccttacgtt attagtacgg ttacgttcct atcgatgccc ggttcgggca tgaatcgtga
     3541 gggggcgagg gatggagccg aggtactcga tggcggctta cgcggcggtc gtgggcgccg
     3601 tcctggtgct gctcgcgctg tcgggcgagc acagtggagc ggcctgggcg gccgccggcc
     3661 tgttggcgat cgcgtcggcg ctcgacgtca cctatgtcgc ccaacggcgc cgcctagcgc
     3721 gggaggcgcc ctaccggtcg aagttcgatc gaatggtccg cattcgctgg gtactcgccg
     3781 gaatcgggtc gcttgccctg atcgcggccg cgggtgcact cgacctcgcg ccgctggacg
     3841 gccaagggcg attcgtcgac gcggtgctgc tctgctcgac cgtcggagcg ctgggcgtct
     3901 tcgtctcgag cctcgtggac tggtactgga tccttcccaa ggtgtcgggg atcgtctgcc
     3961 cggcgccgtg cgaggcgcct ggcggcgagc gctgggagtc accgaccggg cactggtact
     4021 ttcaccgcgg tgtcgcgacg ctgctggtaa gcggcatggc gactgggatt tgtggctaca
     4081 tggccgccac cacggacgac caaggcgagg cggcgatctg gggcgtcgtc gcgctggtcg
     4141 cgatcagcac cgccgttgtg aagttcaacg agcaggggat caaggcgctc tggtacgggt
     4201 tcagcccgcc gatccacgtc ggcgacacga tcctcgccga gcgccacacg gagggaccgg
     4261 tcgagcggcg gcgcgcttac gtggtcgacg tctcgctaca aggagcgaga tacaagttcc
     4321 tcatcgacgg gcggtacgcg gggacgcgct tcgggacaaa gggcgaccaa gccccgattc
     4381 cgaacgatga cctcctgggc actccgcgag cggagccgat cgcggtctgc tcggagtcat
     4441 gcacggggat caactggtac tgccgctaca acccttccgc gcacgacaag gtgaggaagt
     4501 gacagccgac gcgccgtggc ttcagccgcc gctcccccgc gccatcttgc gcaggtcgag
     4561 cgcctcgtcg agggtggcct cgtcgacgcc cttctcgagc gccacctcgc ggatcgtccg
     4621 gcgcgaggcg accgcctcct tgacgatctc ggtcgccttg tcgtagccga tgtgggggtt
     4681 caacgctgtg gcgatcgcgg gggtcgactc ggcgttgcgg gtcagcgcct cctcgttggc
     4741 ctcgaggccg tcgatcacct tgtcggcgag cagcgtgcac gcggcggtga ggatcgtgat
     4801 cgactcgagc aggttgcggg cgatcatcgg gacgcgcacg ttgagctcga agttgccctg
     4861 cgagccggcg acggtgatcg ccacgtcgtt gccgatcacc tgggcggcga cctggagcac
     4921 gacctcgggg atcaccgggt tgaccttgcc gggcatgatc gaggagccct tctgcagctc
     4981 gggcagggcg agctcgccga agccggtgcg cgggccggag cccatgatca cgaggtcgtt
     5041 ggcgatcttg gtcagcgaga cggcgacgac cttcagcgcg ccggacagct cgaccagcgc
     5101 gtcgcggttg gcctgcgcct cgaacgggtc ggccggcccc tcgacgtcga tcccggtcgc
     5161 ctcgcgcagc ttgccgcgca cgcgcgcggc gaactcgggg tgggtgttga ggccggtgcc
     5221 ggtcgcggtg cccccgagcg ggatctggga agcgcgcggc agcgcgtcgc cgacacggtc
     5281 gcggccgagc gagacctgct tcgcgtagcc ggcgaactcc tggccgagcg tcaccgggac
     5341 cgcgtccatc atgtgcgtgc ggcgggcctt gacgacgtcg cgccaggcat cggccttgac
     5401 ttcgagcgcg cccgccagct tctcgagcgc gggcagcagc ttggcgtggg tctccgacac
     5461 cgccgccagg tggaccgccg acgggaagac gtcgttggac gactggccca tgttcacgtg
     5521 gtcgttggcg tgcgctccgt cgccggcgag gttggcgatc acctcgttcg agttcatgtt
     5581 cgacgaggtg cccgagccgg tctggaagac gtcgatcggg aactggtcgt cgtgctcgcc
     5641 gcgggcgatc tcgtcgccgg ccgcggcgat ccgctccgcg atcgcgccgt cgagcagccc
     5701 gagctcgccg ttgactcgcg ccgccgcagc cttgatctgg ccgagcatcc gcacgaccgc
     5761 cgtgggcacc cgctcccccg agaccgggaa gttgtcgatc gccttgctcg tctccgagcc
     5821 ccacagctgc tgctcgactg ccattgcgct ccttcggtct acacggggga cggcgtcgca
     5881 ggctattgga gcgtccgcga ccggtgcggt tcgcgcggca tcgtcgcgcg aagcggtgca
     5941 atcccgtcac gggggaggcg caatcgacca acccgtggtg ccatcgtcac caggccgtcg
     6001 ctcgcggagg gaaacccggc gcgacggtcc gacctcgact ggtcccgagg agggactcgc
     6061 ttgcccctga tccacaccga cttcgagagg accgcgaccg agccgagcga gccgccggcg
     6121 aagaagcgct tctgcagccg ctgcggcgag ccggccgtcg acccgcccgc gggcagcgag
     6181 ccgccggtgc tcgagcagcg cgtctgcggg gtctgcggga tggggatcct gctgcgctgc
     6241 aacccggact cgctgcccgg ggccgggagc gccttcctga tcaccagcca cgcgctcgag
     6301 gtcgcggccg tcagcgaggc tgccgagccg atcttcgcgc ccgaggagaa gctgctcggc
     6361 gcgaacctga tggacctcct gaccagcacg atcggcgacc gccggctggc gaagacggtc
     6421 agccgcgccg cgctgcgcgt ccacgaggcg gtgaccgtgc ccgcccggct ggcgggcccg
     6481 aaggctgacg agatcggcac gatggccgcc cggatctcga cctgcggccc cccgcgcgcg
     6541 gcgctgatcg ccgtcgagca gacgcacttc ggccgccgct agctccccgt tcccgccccc
     6601 tgtatggcgg ctacaacgtc cgtccagcgc tgcaactaac gctgcatcag ttgcgcaaat
     6661 gctgtcgaga gggcacaaaa gagcgcagag gctgcgcggt tcgcaacgga accccgttca
     6721 aggtcacggc tctcgcgacc gattggtacg gagcggcaac ccttccgatg ggctgcgcgg
     6781 cagagaaagg aacggctaga tgagcggcat cagaaccaac gcggcggcgg agatcctcgg
     6841 ggtcagcccg aacacgctgc gcagctggga gcgccgcttc aactacccgc agccgcgccg
     6901 caccgcgggc gggcaccgcc agtacgacct gaccgagctc gaggcgctgc gccgcgcgct
     6961 gatggagacg cacaacatct cctcggcgat cgaggtcgcg cgccagcgcg gcgagggccc
     7021 ctcctcgccg agccgcctgc tcgacgcctt cgaccacttc gacgagtccg ccgccgatcg
     7081 cgtgatggag gagagcctcg ccgtgcgctc ggtcgaccgt tccgtcgagg aggtcctgct
     7141 gcccgcggtc gagctggccg agggccgcga cggccgcgag gccgagcagg agctcgcctg
     7201 ccgctgggcg accggctggt tgcacgccgc gcgccgcgtc gttccgccgc cgacccggtc
     7261 gcagggagtg atcctcttcg acgcctcgaa cggcctcgac ctcgagtcgc tgcacgtcca
     7321 ggccctcgag ctggcgatcc ggcgcgccgg cttccgctcg atcctgctca gcttcgacct
     7381 cccgcccgag cgggtcgccc gcgcgatgcg cgccgtcgag ccgaccgcat tcgtcttctg
     7441 cggcggcgag gcgacgctcg aggtgctcgg ccggctcgtc tacaccgtcc gccaggtcgg
     7501 ctccgcggcg cccgtcttcg agtaccgcga ggcgatgccc gtcaccggcg accactcgat
     7561 cccctcgctc ggctcgaccc cgaccgaggc cgccgacaac ctcaagcagt acgtcgacac
     7621 cggcaagatc gagcacaagg tcgtccagga cgaggccgac gcggacgcgg acgccggcaa
     7681 ggccccggcc aagcccgtcc gcctcgccgc tcagcgctga cgccgacccc tcagatgagg
     7741 gatttgcatc gcctgggcta ccccatccct catccgtcgg ccaccggcgc ggggcaatct
     7801 ccccgatgag ggatttgcat cgcctggacg acacagtccc tcagtcgccc cccggcggcg
     7861 cgacgacgat tgcttaggtg atggattggc atcgcccaga cggcacaacc tctcatcgag
     7921 cgcgaacgcg gggtgtcgag ccgagtctca gcgccacggc ggcaagcgtc tcgcggacat
     7981 ggtccggctc gtagcgcact tgcgcgtggg tgaagcgcaa cgtcgagaga cccgccgcgg
     8041 cgtgcgcttg atcgcgcagc cggtcgcgcg cctgtgcggc gggcgttcgg tgatagcgca
     8101 gtccgtctgt ctcgaccacg agaccgaggt ctggccagaa gaagtcgacc tcgaacccgt
     8161 tcagccacgc gcctgtcttc ggctgtccga ggcctgcgct tcgcgcgatc ggaaggaact
     8221 cgcgttccag ctgtgaggca gtgagcacga acgtgtgacg atcgagcagc tgccgtaacg
     8281 ctcgaacacc acggcggccc gcatagcgct cgagcgccga ccgcaacctc tccggatcca
     8341 cgaggtcatg cttgtccgcc gtgttgatcg cggcctcaag attcttcgaa ctcagcatcg
     8401 gcgcgatgtc gagcagcgtc cgctccactg tcgtgaccgg tacgccgtcg gcctgggtcc
     8461 gctcgtccgg gtcgacgagg cgtcggtagg tgatcagtcc gggccgcgaa cgaaccgtgg
     8521 tgggcgcaac ggtcacctcg atcggacccg ccggcgggtc ggcgatcccg tgcagggcgg
     8581 cggcgctgcg gtgcgagagg accgcggtct cgccacagct gctcaccgcc gccatccagc
     8641 gtccccgctg cgacaggtcg ggccggccga ccgcatagac gccacgctga accggatgca
     8701 gccgaccgct gcgcagccgg tgcgccaccg cacccctgtg cagcccgaga tcgagcagtt
     8761 gcgcgcgggt cacgacgccg tgctggcgcc gcgcgagatc ccagagctgc cgtgcgtcgt
     8821 cgcgaggcgg gcgttggctg gtggttccca tacggatcta gggacagacc cggccccagc
     8881 cgccccccta cgccgatgca tgggcccgcc cgatgcgcga ttcgcatcgc ctggacagcc
     8941 caaccgcgca gcggcgacag cgagctgcgg ggcgaatcgc gaaatgtcgg attcgcatcg
     9001 cctggacggc acaaccgcgc agcggcgaca gcgcgctgcg gggcgaatcg cgaaatgtcg
     9061 gagtcgcatc gcctagacgt cccaatccat catcgggcga gacggtcgga cagcgaatgg
     9121 gccgatgagc ggttcgcatc gcggggacga cacaagccat catcgagcgg cacggggcga
     9181 gggacagcat cccgcacttt gcgggaatcg tccgccgccg ggcctagcat cgcggcggat
     9241 gagcttggag gtcgtcgacc cgctggcgca ccgcgccctt caggcgctga tgcgcgccga
     9301 gacatcagta cggcgcgtgc tggccgcgga gctggagcgc gagggcgtct cggcggccgg
     9361 cttctccgcc ctcgtcgtgc tcaccaccgc cggcggctcg ctcgagctgc gcacgctgcg
     9421 ccgccggctc ggctggtcga aggcgaacgc gaccgaggtc accgccacgc tcgaggcacg
     9481 cggcttcatc gcccgccgcc ggtcggccgg cgaccgccgc gccgtccttc tcgacctcac
     9541 cgtcgcgggc gccgaggtgg tggagcggct cttccccggt cacacggagc gcgtgcgctc
     9601 gacgttctcg acgctcgacg aggacgagaa gcggtcgctc gcctccctct gccgcaagct
     9661 ggccgcctga cgccagcaga ggtgcgcccg ccaccaggcc ccgccggagg gtggcgcctc
     9721 atgcatgaca tacgtgcaaa aagcgccacg gtcccatggt gagggccgaa tcgctcgagg
     9781 ctcgccgagc ggccgcagac cgtccataga atcggccgcg tggccaggtt
//
LOCUS       DATABV010000059         4804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_981821, whole genome shotgun sequence.
ACCESSION   DATABV010000059 DATABV010000000
VERSION     DATABV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981821"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VHF88_02365"
     CDS             complement(<1..684)
                     /locus_tag="VHF88_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2160642.1"
                     /translation="MNTAEQLAPVLAAGGPGLARGLRLAEARLGEVAGAHGEALAGHV
                     GGTLAAGGKRLRPLLVFLCAGHDEPDERVVAGAAAVELLHMATLVHDDVLDRATLRRG
                     YPTVFAGAGRGAATATGDLLFSRAFAELAATGSAAAVQALSAASTALARGELMQRDDA
                     WSAAVTPDRYRVRCGLKTGSLFAASCRLGAELGARGPESADALAGFGDRLGVAFQILD
                     DVLDVSGPVE"
     gene            complement(681..1400)
                     /locus_tag="VHF88_02370"
     CDS             complement(681..1400)
                     /locus_tag="VHF88_02370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX2160643.1"
                     /translation="MGAGAQRAGDTLPEHQVAAMFDRVSRFYDLINSVMSAGLHHAWR
                     VRAADIAKVGPGDRVLDVAAGTGDLTIELNKRVVPNGEVVGVDISEGMLTLAREKAPG
                     IRFEHGSALDLRYADGEFDAATTGFAARNFSDLRRGLAEMRRVVRPGGRVVVLDFTTP
                     TRPPLSWFFQLWFDRIVPLLGRFSDDPEAYMYLPNSVKRFPPSRELAAELAAVGLTDV
                     RWMATAGGIVTVHSGVVPPAR"
     gene            complement(1414..2178)
                     /locus_tag="VHF88_02375"
     CDS             complement(1414..2178)
                     /locus_tag="VHF88_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX2160644.1"
                     /translation="MNAREKEQYLREYSILKSQGKPFFPYAVAKDGAMAMVTLAVIIL
                     MAVLFGAELGPKADPTTTTYSPRPEWYFFFLFELLRVVKPPELVFAATIGIPTIALML
                     LLLLPFYDRNPERHPLKRPIAMIGAVATVAAMGYLTVLGALAGPPSEIDIEVSSQYQR
                     GADVAAASGCLGCHRIGHNGNTLGPDLTEIGDRLGRDAIARTLINPTAPMPSYQSLRE
                     SEPKQFEELVTFISSLREHNEAAEPEGAGESSSSKN"
     gene            complement(2180..2977)
                     /locus_tag="VHF88_02380"
     CDS             complement(2180..2977)
                     /locus_tag="VHF88_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX2160645.1"
                     /translation="MSLVPKFMRPAPSRPAERPGPAPTPLERAKSQGAEAGAGVVGYL
                     DERTGASPFLRGFLFRKVPKGTNWFYTLGSATMFAFVSQAVTGVFLAMYYEPDPTRAY
                     TSAAHITNEVFLGELVRGMHRWGATVMIVLLFLHMGRTFVFGAYKYPRELNWVIGVVL
                     TVLTLMMGLTGYLLPFDQRSFWATVVGVNINASGPIMGPYLADFMRGGAEFGATTLTR
                     FYALHMLLIPGLIAALIGAHLYLVAKLGTSAPPWLKAEKKSLKREEI"
     gene            complement(2974..3639)
                     /locus_tag="VHF88_02385"
     CDS             complement(2974..3639)
                     /locus_tag="VHF88_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX2160646.1"
                     /translation="MAQLPDKHKRKSRYTVDRNIPGAFEGETVTRRTFMTAGALTYGG
                     IASAMFGLPALGFALGPMFERSAPETWQDVGSEEDFNPETYVPVVMNLVPSIGEAGKT
                     TVYIRRFDPGRDAERNADLPYVAISTRCAHLGCPVRYIQASQRFVCPCHGGVYDFEGK
                     VDGGPPVRPLDRFYTRVRNGRVEVGDRFSVNSELERFPHPRDPSNHLDGLWQYLYPAR
                     PST"
     gene            complement(3686..4060)
                     /locus_tag="VHF88_02390"
     CDS             complement(3686..4060)
                     /locus_tag="VHF88_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160647.1"
                     /translation="MSALRAVVAAVDPALAANAVADPGPDRFAGVVTDPDRLFTIEAV
                     HEGYLLHYGEPRAFDGMDADLRLLAGDALYALGLSRVADKGDLEAVAELSDLITAAAR
                     AHAEDDPARAVALWADSAQRLA"
     gene            complement(4057..>4804)
                     /locus_tag="VHF88_02395"
     CDS             complement(4057..>4804)
                     /locus_tag="VHF88_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2160648.1"
                     /translation="WVAPVPTRALLALLIALIAVLAAVPPAGAAGADGGPAGESFLAF
                     LREAADRNGALLVFDEVISGFRVGRGGAQERERVTPDLTVLGKVIGGGLPAAAYAGRR
                     DLMERVAPAGDVYQAGTLSGNPLATAAGLATLALLDDDAYARLDAVAATLADGLRAAG
                     AEVGVPVHVSRTTGLLTLFFHDGPVSDYAGAKAADADRFAAFCRAMLAGGVYLPASQF
                     ETWFPSIVHSDEQVERTLAAAADALREVAP"
BASE COUNT          717 a         1646 c         1818 g          623 t
ORIGIN      
        1 ctcgaccggc cccgagacgt cgagcacgtc gtcgaggatc tggaacgcga cgccgaggcg
       61 gtcgccgaag cccgcgagcg cgtcggcgct ctccggcccg cgcgcgccga gctccgcgcc
      121 gagccggcac gacgcggcga acaggctgcc ggtcttcagg ccgcagcgca cgcggtagcg
      181 gtcgggcgtc acggcggcgg accaggcgtc gtcgcgctgc atcagctcgc cgcgcgcgag
      241 ggcggtcgaa gcggcggaga gcgcctgcac ggcggccgcg ctgccggtcg ccgccagctc
      301 cgcgaaggca cgcgagaaca gaaggtcgcc ggtggcggtc gcggcgccac ggccggcgcc
      361 cgcgaagacg gtcggatagc cgcgccgcag cgtcgccctg tccagcacgt cgtcgtggac
      421 cagtgtcgcc atgtgcagca gctcgaccgc ggcggcgccc gccacgacac gctcgtccgg
      481 ctcgtcatgg ccggcgcaga ggaacaccag cagcggccgc agccgcttgc cgcccgcggc
      541 gagagtgccg ccgacatgcc ccgcgagggc ctcgccgtgg gcgccggcga cctcgcccag
      601 gcgcgcctcg gcgagccgca gcccgcgcgc cagccccggt cccccggccg cgaggaccgg
      661 ggcgagctgc tcagcggtgt tcatcgcgcc ggcgggacga ctccagagtg aacggtgacg
      721 attcccccgg cggtggccat ccaacgaacg tcggtcaggc cgacggccgc cagctcggcg
      781 gccagctcgc gcgagggcgg gaagcgcttg accgagttcg ggaggtacat gtaggcctcc
      841 gggtcgtcgg agaagcgtcc gagcagcggc acgatccggt cgaaccagag ctggaagaac
      901 caggacagcg gcggccgcgt cggcgtcgtg aaatcgagca cgacgacgcg gccgccggga
      961 cggacgacgc gacgcatctc ggccaggccg cggcgcaggt cggaaaagtt gcgggcggcg
     1021 aagccggtcg ttgccgcgtc gaactctcca tccgcgtacc gcaggtcgag cgcgctgccg
     1081 tgctcgaagc gaatgccggg cgccttctcc cgcgcgagcg tgagcatacc ctccgagatg
     1141 tcaacgccca cgacctcgcc gttgggcacg acacgcttgt tcagctcgat ggtcaggtca
     1201 ccggtgcccg cggcaacgtc gagcactcgg tcccccggac ccaccttggc gatgtcggcg
     1261 gcgcgcacgc gccaggcgtg gtgcaggccg gccgacatga cggagttgat caggtcgtaa
     1321 aagcgtgaga cgcgatcgaa catcgccgcg acctggtgct ccggcaaagt gtcgccggct
     1381 cgttgcgcac cagcgcccat cgagcggcgc ccctcagttc ttcgacgaag acgactcgcc
     1441 ggcgccctcg ggctcggcgg cctcgttgtg ctcgcgcagc gaggagatga aggtgacgag
     1501 ctcctcgaac tgcttcggct cgctctcgcg caggctctgg tacgacggca tcggcgcggt
     1561 cgggttgatc agcgtgcggg cgatcgcgtc gcggccgagg cggtcgccga tctcggtcaa
     1621 atccggcccg agcgtgttgc cgttgtggcc gatccggtgg cagccgaggc agcccgaggc
     1681 cgcggccaca tcggcgccgc gctggtactg cgacgagacc tcgatgtcga tctcgctcgg
     1741 cgggccggcg agcgcgccga ggacggtcag gtagcccatc gccgcgacgg tcgcgacagc
     1801 gccgatcatc gcgatcggcc gcttgagcgg atggcgctcc gggttgcggt cgtagaacgg
     1861 cagcagcagg agcagcatca gcgcgatcgt cgggatgccg atcgtcgccg cgaacacgag
     1921 ctccggcggc ttgacgacgc gcagcagctc gaagaggaag aagaagtacc actcggggcg
     1981 cggcgagtag gtggtcgtgg tcgggtccgc cttcgggcct agctcggcgc cgaagaggac
     2041 ggccatcaag atgatcaccg cgagcgtgac catcgccatc gcgccgtcct tggcgaccgc
     2101 gtaggggaag aacggcttgc cctggctctt gaggatcgag tactcgcgca ggtactgctc
     2161 cttctcgcgc gcgttcacct cagatctcct cgcgcttgag tgacttcttc tcggccttca
     2221 gccacggcgg cgcgctcgtg ccgagcttcg ccacgaggta gaggtgcgcg ccgatcagcg
     2281 cggcgatcag gcccgggatc agcagcatgt gcagcgcgta gaagcgggtc agcgtggtcg
     2341 ccccgaactc ggccccgccg cgcatgaagt cggccaggta cgggcccatg atcgggccgg
     2401 aggcgttgat gttgacgccg acgaccgtcg cccagaacga ccgctggtcg aacggcagca
     2461 ggtagccggt caggcccatc atcagcgtca ggacggtcag cacgacgccg atcacccagt
     2521 tcagctcgcg cgggtacttg taggcgccga agacgaacgt gcgccccatg tgcaggaaca
     2581 gcaggacgat catcacggtc gcgccccagc ggtgcatgcc gcggaccagc tcgccgagga
     2641 agacctcgtt ggtgatgtgg gccgccgacg tgtaggcgcg cgtcgggtcc ggctcgtagt
     2701 acatcgcgag gaagacgccg gtcacggcct gcgagacgaa ggcgaacatc gtcgccgagc
     2761 ccagcgtgta gaaccagttc gtgcccttcg gcaccttgcg gaagaggaag ccgcgcagga
     2821 acgggctggc gccggtccgc tcgtcgaggt agcccacgac gccggcgccg gcctcggcgc
     2881 cctggctctt ggcgcgctcg agcggggtcg gcgcgggccc cgggcgctcg gccggacgcg
     2941 acggcgcggg acgcatgaac ttcggcacga ggctcacgtg ctcggcctcg ccgggtagag
     3001 gtactgccag agcccgtcca ggtggttcga cgggtcgcgc gggtgcggga agcgctcgag
     3061 ctcggagttg accgagaagc ggtcgccgac ctcgacgcgg ccgttgcgca cgcgcgtgta
     3121 gaagcggtcg agcggtcgca ccggcgggcc gccgtcgacc ttgccctcga agtcgtagac
     3181 gccgccgtgg cacgggcaga cgaagcgctg cgaggcctgg atgtagcgga ccgggcagcc
     3241 gaggtgggcg cagcgggtcg agatcgcgac atagggcagg tcggcgttgc gctcggcgtc
     3301 gcgaccgggg tcgaagcggc ggatgtagac ggtggtcttg ccggcctcgc cgatcgacgg
     3361 cacgaggttc atcacgaccg ggacgtaggt ctcggggttg aagtcctcct cgctgccgac
     3421 gtcctgccag gtctcggggg cgctgcgctc gaacatcggg ccgagcgcga agccgagcgc
     3481 cggcaggccg aacatcgccg aggcgatgcc gccgtaggtc agcgcaccgg cggtcatgaa
     3541 ggtgcgccgc gtaacggtct caccctcgaa cgcaccggga atgttgcggt cgacggtgta
     3601 gcgcgacttg cgcttgtgct tgtcaggaag ctgagccact gctggggagg taggcgcccg
     3661 tcgggcgcgc gaccaccaat tctaattagg cgagccgttg ggcgctgtca gcccagagtg
     3721 cgaccgctct ggcggggtcg tcctcggcat gcgcgcgggc ggccgcggtg atcaggtcgg
     3781 acagctccgc gaccgcctcg agatcgccct tgtctgccac gcggctcagt ccgagcgcgt
     3841 agagcgcgtc gccggcgagc aggcgcaggt cggcgtccat cccgtcgaac gcgcgcggct
     3901 cgccgtagtg gagcaggtag ccctcgtgga cggcctcgat cgtgaacagg cggtcgggat
     3961 cggtaaccac gccggcgaag cggtcgggac cggggtcggc gacggcattt gcggcgagcg
     4021 cgggatcgac cgccgccacg acagcgcgca gggcgctcac ggcgcgacct cgcgcagcgc
     4081 gtcggcggct gcggcgagcg tgcgctcgac ctgctcgtcg ctgtggacga tcgacgggaa
     4141 ccaggtctcg aactgcgatg ccggcaggta gacgccgccc gcgagcatcg cacggcagaa
     4201 cgcggcgaag cggtcggcgt cggcggcctt ggcgccggcg tagtcgctca ccggtccgtc
     4261 gtggaagaag agcgtcagca gcccggtggt gcggctgacg tggaccggga cgccgacctc
     4321 ggcgcccgct gcgcgcagcc cgtcggcgag tgtggcggcg accgcgtcga ggcgggcgta
     4381 ggcgtcgtcg tcgagcagcg cgagcgtcgc gagtccggcc gcggtggcga gcgggttgcc
     4441 cgacagcgtt ccggcctggt agacgtcgcc ggccggcgcg acgcgctcca tcaggtcgcg
     4501 gcgaccggcg tacgcggccg cgggcaagcc gccgccgatc accttgccga gcaccgtcag
     4561 gtcgggcgtc acccgctcgc gctcctgggc gccgccgcgg ccgacgcgga agccgctgat
     4621 cacctcgtcg aagaccagca gcgcgccgtt gcggtcggcc gcctcgcgca ggaaggcgag
     4681 gaacgactcg ccggccggcc cgccgtcggc gccggcggcg ccggcgggcg ggacggccgc
     4741 gagcacggcg atcagggcga tcagcagggc gagaagcgcc cgggtgggga ccggggcgac
     4801 ccat
//
LOCUS       DATABV010000060         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1302843, whole genome shotgun sequence.
ACCESSION   DATABV010000060 DATABV010000000
VERSION     DATABV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302843"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VHF88_02400"
     CDS             <1..1036
                     /locus_tag="VHF88_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX2160649.1"
                     /translation="IPSRANGRGLREVGVSPTLAPGLADAPTAGLGAAAIGRALADGE
                     LSTLLLLARDPFTELSDRPTWERALDRADAVVAFADFVTPQLAEHATVVFPGESNAEK
                     EGTLTHPDGRLQRVRQAVPHADEARPVWAVLAQLCERAGAPLTPPTNGAGPAAGGSVV
                     ATNGAAPALSLPSVTAAVAAAVPFYAGITLDEIGGRGIRWQDRDAASAAPAAELRTGP
                     LAAPPELPAGLRLGAVPALFASPAVQHSPSLRFLAPRQRVELSPADAERLGIAAGDDV
                     EVTSGDRSVRATAALRDTVRPGSVFLLQGLGESGAEALTNGVPRTVEVRSAPPLIHQV
                     GAATDEADPA"
     gene            1033..2028
                     /gene="nuoH"
                     /locus_tag="VHF88_02405"
     CDS             1033..2028
                     /gene="nuoH"
                     /locus_tag="VHF88_02405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX2160650.1"
                     /translation="MTVESTLVQIVKAIVIFAFILQVVPLILLLERKLLGRFQSRYGP
                     NRVGFRGLGQPLADVLKLLSKESFTPATAVPWMMAIAPVISIATAVATLAIIPFGPQG
                     AWGGDLGLYGIDVPIGLLYFFAFGSLAFYALVLGGWASGSKYSFLGSMRSAAQLISYE
                     VAIGLSLLGVAMMAGSLSFVDIVEAQGADGKGIWFIVPQFVGFLVFIVAGFAETARAP
                     FDLPEADAEIVAGYNTEYGGMRFGSFFMAEYIEVIIIAGVATACFFGGWHGPGPIALA
                     PLWVLLKMFAFVVLFIWVRATLPRLRYDQLMSFGWKVLLPLATLNALVTAIVLVV"
     gene            2035..2568
                     /gene="nuoI"
                     /locus_tag="VHF88_02410"
     CDS             2035..2568
                     /gene="nuoI"
                     /locus_tag="VHF88_02410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX2160651.1"
                     /translation="MSYELTDDVIEVQRGPEPGGLGGAYRTFGATLRGLRTTLGRLAE
                     KPVTIEYPEEKTPVYPRFRGRHRLQRFEDTGLEKCVGCSLCVAACPAQCIRVVADENT
                     PEHQVSAGERYAAVYEISMSRCIFCGYCEIACPFDAITMGHDYEMADYERSDLIFTKE
                     MLLAEPIERTPLRREGE"
     gene            2583..3185
                     /locus_tag="VHF88_02415"
     CDS             2583..3185
                     /locus_tag="VHF88_02415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX2160652.1"
                     /translation="MFFEQLLFFVAGAGAIGGALGVVLLRNPFYSVLALVAHLISLAI
                     LFLLLTAAFLAAAQVVVYAGAVMVLYVFVVAYVGGTDDELLPRPAGVAALVGPLFAAA
                     LLIELTIAVVGSGLSALDSRGAEVGAGFGSPGAIGDALLNKYLLAFEAASFLLLIAAV
                     GAVVLARKRRGLQEPADAISVADVVRHQPPAAGTQKEAIG"
     gene            3186..3485
                     /gene="nuoK"
                     /locus_tag="VHF88_02420"
     CDS             3186..3485
                     /gene="nuoK"
                     /locus_tag="VHF88_02420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX2160653.1"
                     /translation="MDIGWFVVLSAFIFCIGAAGVMTRRSPLVILLCLELMLNAGNLA
                     LIAFSRMVGNHEGQVFALIVMVVAACEVVIGLGIIVAMYRRRLAIDVDDMAELRG"
     gene            3482..>4555
                     /locus_tag="VHF88_02425"
     CDS             3482..>4555
                     /locus_tag="VHF88_02425"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX2160654.1"
                     /translation="MSATSWGWLVLALPLAGTLAIGLGWRMLGDRVAGWLGSAAIGGA
                     FAASIAMLLALLDEPEESRSLVDSAWTYAATAGFGVDMAILVDPLSVFMCLVVTGVSF
                     LIHVYSVAYMESDRSYKRFFAYLNFFVFSMLLLVLAANFVLLIVGWAFVGAASYLLIS
                     FWYRRTTATAAGIKAFVINVIGDVGLVIGAFLLFDATGALDYAGVFGQVEQAFGQNDG
                     QLVAACLLLLVGAFAKSAQLPLHTWLPDAMEGPTPVSALIHAATMVTAGVYLIARMHP
                     LFEQAPAAADISAVIGTLTLFVAATIALVASDLKRAIAYSTMSQIGYMILGVSVFAYG
                     AGLFHLMTHAFFKALLFMGAGSVI"
BASE COUNT          528 a         1677 c         1565 g          785 t
ORIGIN      
        1 gattccgagc cgtgcgaacg gccgcggcct gcgcgaggtc ggcgtctcgc cgacgctggc
       61 gcccggcctg gccgacgcac cgacagctgg cctcggcgct gccgcgatcg gccgcgcgct
      121 cgccgacggc gagctgtcga cgctgctgct gctcgcgcgc gacccgttca ccgagctgtc
      181 cgaccgcccg acctgggagc gcgcgctcga ccgcgccgac gccgtcgtcg cgttcgccga
      241 cttcgtcacg ccgcagctcg ccgagcacgc caccgtcgtc ttcccgggcg agtcgaacgc
      301 cgagaaggag gggacgctca cccatcccga tggtcgcctg cagcgcgtcc ggcaggcggt
      361 cccgcacgcc gacgaggcgc ggcccgtatg ggcggtgctc gcccagctct gtgagcgcgc
      421 gggcgcgccg ctgacgcccc cgacgaacgg cgccggccca gcggccggcg gctccgtcgt
      481 cgctacgaac ggcgccgcac ccgcgctctc gctgccgagc gtcaccgcgg ccgtcgccgc
      541 cgccgtcccc ttctacgccg gcatcacgct cgacgagatc ggcgggcgcg gtatccgctg
      601 gcaggaccgc gacgcggcgt ccgccgcacc agccgccgag ctgcgcaccg ggccgctcgc
      661 cgcgccgccc gagctgccgg ccggtttgcg cctcggcgcc gtgccggcgc tgttcgcctc
      721 gccggccgtc caacactcgc cgtcgctgcg cttcctcgcg ccgcgccagc gcgtcgagct
      781 gtcgcccgcc gacgccgagc ggctcggcat cgccgctggc gacgacgtgg aggtcaccag
      841 cggcgaccgc tccgtgcgcg cgaccgccgc cctgcgcgac acggtccgcc ctggcagcgt
      901 cttcctgctc caaggactgg gcgagagcgg cgccgaggcg ctcaccaacg gcgtcccgcg
      961 cacggtcgag gtccgctcgg cgccgccgct gatccatcag gtcggagccg cgaccgacga
     1021 ggccgatccc gcatgaccgt cgagtcgacg ctggtccaga tcgtcaaggc gatcgtgatc
     1081 ttcgcgttca tcctgcaggt cgtcccgctg atcctgctgc tcgagcgcaa gctgctcggc
     1141 cgcttccagt cgcgctacgg gccgaaccgc gtcggcttcc gcgggctcgg tcagccgctc
     1201 gccgacgtgc tcaagctgct gtcgaaggag agcttcacgc cggcgactgc ggtcccgtgg
     1261 atgatggcga tcgcgccggt gatctcgatc gccacagcgg tcgcgacgct cgcgatcatc
     1321 ccgttcggcc cgcagggcgc ctggggcggc gacctcggcc tctacggcat cgacgtcccg
     1381 atcggactgc tctacttctt cgccttcggc tcgctcgcct tctacgcgct cgtgctcggc
     1441 ggctgggcgt cgggctcgaa gtactcgttc ctcggctcga tgcgctcggc ggcgcagctg
     1501 atctcctacg aggtcgcgat cggcctgtcg ctgctcggcg tcgcgatgat ggccggctcg
     1561 ctgtcgttcg tcgacatcgt cgaggcgcag ggcgccgacg gcaaggggat ctggttcatc
     1621 gtcccgcagt tcgtcggctt cctcgtcttc atcgtggctg gcttcgcgga gacggcgcgc
     1681 gccccgttcg acctgccgga ggccgacgcg gagatcgtcg cgggctacaa caccgagtac
     1741 ggcgggatgc gcttcggctc gttcttcatg gccgagtaca tcgaggtgat catcatcgcg
     1801 ggcgttgcga ccgcctgctt cttcggcggc tggcacgggc cggggccgat cgcgctcgcc
     1861 ccgctctggg tgctgctgaa gatgttcgcc ttcgtagtcc tcttcatctg ggttcgtgcg
     1921 acgcttccgc ggctgcgcta cgaccagctg atgtcgttcg gctggaaggt gctgctgccg
     1981 ctggccacgc tgaacgccct cgtcaccgcg atcgtcctgg tggtctgacc aaacatgagc
     2041 tacgagctga cagacgacgt catcgaggtc cagcgcgggc ctgagcccgg cgggctcggc
     2101 ggcgcctacc gcaccttcgg ggcgacgctg cgcggcctgc gcacgacgct cgggcggctg
     2161 gccgagaagc cggtcacgat cgagtacccg gaggagaaga cgccggtcta cccgcgcttc
     2221 cgcggccgcc accggctgca gcgcttcgag gacacggggc tcgagaagtg cgtcggctgc
     2281 tcgctctgcg tggcggcctg cccggcgcag tgcatccgcg tcgtcgccga cgagaacacc
     2341 cccgagcacc aggtgtcggc cggcgagcgc tacgcggcgg tctacgagat cagcatgagc
     2401 cgctgcatct tctgcggcta ctgcgagatc gcctgtccct tcgacgcgat cacgatgggc
     2461 cacgactacg agatggccga ctacgagcgc tccgacctga ttttcaccaa ggagatgctg
     2521 ctggccgagc cgatcgagcg gacgccgctg cgccgcgagg gcgagtaggg ggcggcacca
     2581 acgtgttctt cgagcagctc ctcttcttcg tggcgggcgc gggcgcgatc ggcggcgcgc
     2641 tcggcgtcgt gctgctgcgc aaccccttct acagcgtgct ggcgctggtc gcgcacctga
     2701 tctcgctggc gatcctgttc ctgctgctga ccgccgcctt cctcgccgcc gcgcaggtcg
     2761 tcgtctacgc gggcgcggtg atggtcctct acgtcttcgt cgtcgcctac gtcggcggca
     2821 ccgacgacga gctgctcccg cgcccggccg gtgtcgcggc gctcgtcgga ccgctgttcg
     2881 ccgcggcgct gctgatcgag ctgacgatcg ccgtcgtggg ctccggcctc tccgcgctcg
     2941 acagccgcgg tgccgaggtc ggcgccggct tcggctcgcc gggcgcgatc ggcgacgccc
     3001 tgctgaacaa gtacctgctc gcgttcgagg ccgcctcctt cctgctgctg atcgccgccg
     3061 tcggcgctgt cgtgctcgcg cgcaagcggc gcggcctgca ggagccggcc gacgcgatct
     3121 cggtcgccga cgtcgtccgt caccagccgc ccgccgcagg cacccagaag gaggcgatcg
     3181 gctagatgga catcggctgg ttcgtcgtcc tgtcggcgtt catcttctgc atcggcgcgg
     3241 ccggcgtgat gacgcgccgc agcccgctgg tgatcctgct ctgcctcgag ctgatgctca
     3301 acgccggcaa cctcgcgctg atcgcgttct cgcgaatggt cggcaaccac gagggccagg
     3361 tcttcgcgct gatcgtgatg gtcgtcgcgg cctgtgaggt cgtgatcggc ctcgggatca
     3421 tcgtcgcgat gtaccgccgg cgcctcgcca tagacgtcga cgacatggcg gagctgcgcg
     3481 ggtgagcgcc acgtcgtggg gttggctggt gctggcgctc ccgctcgcgg ggacgctggc
     3541 gatcgggctc ggctggcgca tgctgggcga ccgcgtcgca ggctggctcg gcagcgcggc
     3601 gatcggcggc gcgttcgccg cgtcgatcgc gatgctgctc gccctgctcg acgagccgga
     3661 ggagtcgcgc tcgctggtcg actccgcctg gacctacgcc gcgacggccg gcttcggcgt
     3721 cgacatggcg atcctcgtcg acccgctgtc ggtcttcatg tgcctggtcg tcaccggcgt
     3781 gtcgttcctg atccacgtct actcggtcgc ctacatggag tcggaccgca gctacaagcg
     3841 gttcttcgcc tacctcaact tctttgtctt ctcgatgctg ctgctcgtcc tggcggcgaa
     3901 cttcgtgctg ctgatcgtcg gctgggcgtt cgtcggcgcc gcgtcctacc tgctgatctc
     3961 gttctggtac cgccgcacga ccgcgacggc cgccggcatc aaggcgttcg tgatcaacgt
     4021 gatcggcgac gtcggcctgg tgatcggcgc cttcctgctc ttcgacgcga ccggtgcgct
     4081 cgactacgcc ggggtcttcg gccaggtcga gcaggccttc ggccagaacg acggccagct
     4141 cgtcgccgcc tgcctgctgc tgctcgtcgg cgcgttcgcg aagtcggccc agctgccgct
     4201 gcacacctgg ctgcccgacg cgatggaggg cccgacgccg gtctcggcgc tgatccacgc
     4261 ggcgacgatg gtcaccgccg gcgtctacct gatcgcgcgc atgcacccgc tcttcgagca
     4321 ggcgccggcc gcggccgaca tcagcgcggt gatcgggacg ctgacgctgt tcgtggcggc
     4381 gacgatcgcg ctcgtcgcga gcgacctcaa gcgggcgatc gcctactcga cgatgtcgca
     4441 gatcggctac atgatcctcg gcgtctcggt cttcgcctac ggcgccgggc tgttccacct
     4501 gatgacccac gccttcttca aggcgctgct gttcatgggc gccggctcgg tgatc
//
LOCUS       DATABV010000061        17969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1142403, whole genome shotgun sequence.
ACCESSION   DATABV010000061 DATABV010000000
VERSION     DATABV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17969
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142403"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="VHF88_02430"
     CDS             <1..1475
                     /locus_tag="VHF88_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160655.1"
                     /translation="AVRGKPAEALSTVDDLPYVESVNGIGVAADGSVYVAKSRSLSSY
                     DPGSGFERCRSPRGLRACQGLMVLASGGPRTAALEGVVRVGETTASLEGKVQPRGAAR
                     YRFQVSDGGRWRDVGKPRYVSGPREVRVSTLARGLRPGALYRARLLLATRDRDGEEDR
                     VASNIAFLVTVRPEAFATARPARAFAPLVQLHSDERSFPISASRFLQRATVKWKDGHC
                     IGLINVATGRIASRKTPGAARVRPSRLGRAARPYRHRAFDRKCERRRPPVYSAIDLTR
                     PFHRGRRAAGLPRDSGFYLDLLSDSYDGDPRFARRGEQRLLVGASAYYERRRAVVEGR
                     PGIRLAYWLLYGSSRLPRVRGEPIGQHEGDWERIDVLARPGSRRHRWLPRAVRFHVGD
                     RHRTLPWRDVLRQRSHPVVFAALGSHRPYPHAGRYELSARVAGRRARLLDETAACARC
                     PQWETWKRLGPVRRQPWYGFGGGWGLAFRHHDTSGPLGPR"
     gene            complement(1500..2090)
                     /locus_tag="VHF88_02435"
     CDS             complement(1500..2090)
                     /locus_tag="VHF88_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160656.1"
                     /translation="MSRKYKVMLLLAFAIVAFALPTSYASSQEPVEVTAEGSDHCSLI
                     INYCPLELHGESHMSIFGFIISNCEDEFLLELDENGTGFVALAFMDDHSDGGDCTRVR
                     CNGVSEPQSEIEWRITNVRESQLERVLMDVDFCLDAEGNPDGPGTHCNAPLLVSETTG
                     THQYRFFTSYTCPNGVRIEGSWLPEGSPVEYEHDVP"
     gene            complement(2110..3135)
                     /locus_tag="VHF88_02440"
     CDS             complement(2110..3135)
                     /locus_tag="VHF88_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160657.1"
                     /translation="MAAPSAAHEPVELSHEDGPHCDIAANPCIVEAHGESHLSVMGFL
                     VSSCESHFVAELGEDGTGHVTDVDLIDHPNSGVCTRIPCNGVGESPSEVEWDIANTRE
                     TAVNQVTMDRTFCIDASNNPNGTGMHCTAPITITETGTHQYAFSMGFTCPNGGRFESE
                     WAAEGTSVEFEHAVPPGQEPVEVDRGDCQLETDPCVLDVHGESHLSIFGFIVSNCEEE
                     FTTHIGADGSGHVVNAVLLDHSDGGDCTRAPCNGIGESADEVEWDMTAIEETWPNTGL
                     ANLNFCLDAQGNPSGTGTHCNAPVSLTEGPELHHYALSAAYTCPSGVRLEGTWEGEGA
                     PLEVTHQ"
     gene            complement(3188..3775)
                     /locus_tag="VHF88_02445"
     CDS             complement(3188..3775)
                     /locus_tag="VHF88_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160658.1"
                     /translation="MSVAVKIPPLCAAALASITLASSAWAQEPVALEHEGGAHCDIST
                     NPCQLTAHGEWHISSFGFIIFICEDEFTAQLGEDGKGHTTSATIFDHSDGGDCPRVPC
                     NGVGESSAEVEWDIVNARETAPGETTFDRNLCIDAQGSPNATGTHCSTPMSLRETQGT
                     HHYSLSMAYTCPNGIRLEGDWAVEGTPIEVAHDAP"
     gene            complement(3862..4455)
                     /locus_tag="VHF88_02450"
     CDS             complement(3862..4455)
                     /locus_tag="VHF88_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160659.1"
                     /translation="MRIARKLALLCAMAFAAMALAASAASAQVPVEVEHEGGAHCDIS
                     ANPCDVVAHGESHLSVFGFIVSNCEDEFTASIGEDGTGHITNASLVDHSDGGDCTRVP
                     CNGVGEGSGEVEWDITNTRETGTDVGIMDVNFCLDAAGNPNGTGTHCNAPITVRETTG
                     THHYTFSTGFICPNGVRVEGNWEAEGTPIEIEHDVAQ"
     gene            4904..9874
                     /locus_tag="VHF88_02455"
     CDS             4904..9874
                     /locus_tag="VHF88_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160660.1"
                     /translation="MGLGATIVVAAVLGLAASALATLYVGSFFGNTGNTGGLFNTPNG
                     VGVHNASGNAYVADRLNHRVQQFNASGQFVRAWGRDVVSAGPGNADEQQLATVNATAG
                     TYTLTYSGQTTVPIAFDAPASAVQTALEDLSNLAPGDVTVSGGPGGVGGASPYVIQFG
                     GTLANVNVAQITASSAGLTGTATTATANEGATGFEICVAANGDTCKAGTTTGGIGGAF
                     NNPLGVAVNQGTGDVYVSDGSASHRRVQQFTSTGQFIRAWGQDVIAGTAPNSNGTGFE
                     ICDTTAASANFHNQCKAGLSQSTGGAFGTISAANGGYLAVVPAGAPNAGNVVVADPGN
                     NRVQEFTASGAFVRAFGGDVENPPGGSAFEVCTIAANCKAGAPGTAVGQFDASFPRRV
                     AADATGAIYTVESSVAARRVQKFTPQALPPGLAPSLFADAIVGTSGEFPLEPLDVAVD
                     PVSGRVFVTKTYGAGTFTCPDGSPSIPTEVRVVELSSSGTQLDSHAGCAGFTFSNVLG
                     LAFRPSAGGGDSERLYISSTNGGHRVYVLDQDGITAAIANVNPATDITSTTALLSATV
                     NPNSVDNSFAPTVWRIEYSRNGATWTTANSGSLPAGTTPETVTAEVSNLLPNTTYQVR
                     VVTRKPLGNPEVVSPELTFRTDAVRPEIGTVGADTITSTSARLTGQIVPHSTPTTYRF
                     EWGQGSFSNVTPVPDGSAGAGPTAVFVFQQLAGLQPGTTYQFRLVATSQTEGATSSET
                     RTFTTLSPGARADVDARGYELVSPPDKVGGTGVGEWYQGPGSMGSAGLAAYEGERFAA
                     NGAFGSTLMDGAHAWANDWAFAQRIGDGVGWQSHSPLTHPNYRATLAGKLNLVSTTPA
                     LSRFFWHSPQGTPAMFREIANEPGWGTPAGLFSDWGAPTRWEVFGPADPLDEDNPDND
                     QMLGSTSNLIWGVGFSDDGSLAAAVTELNAGKVVGLGGPSDPTTPRPMAPSGDLVEGR
                     SIYLADTSNTLRDAFDPAVQRTLANVCTGAGATRTEVPAVDLTGNLTAEACTDDPGDD
                     LISNYGATLRGGPGANDPVRVDDVVNADGSRVFFMSPDPAAPSVPNGLTSFCSTVGGT
                     CPTQLYVRQQSADGSNVTTRWISKAEDGLFGTQDSTLTGTVRFEGASRDGDKVFFRTN
                     SPLTADDPNGGCGAPCTTGNASPNSWDLYLYDLPDAPGADPGDGDLTRISAGPGGDGD
                     CNSPLGGDGDVGALRFASEDGGRVYFTCAAPLAGVGAPSNGTVTTPGGSQTTPGQTNL
                     YVYDTSRDADDRWRFVARIPRSTDRTIETCASTGQDRRSPLAAPAQAPSTFVIEAAAA
                     DANCLSGTSDGAFVTFFTTGRLTADDPTSPVTGDVYGYDAAADDLVRITAPQGGVGET
                     YPCAPGNSAVACFGDSGVGVQPGIGSGTSTAPLGVATDPLVGGDRVAFLQSRSRLVPE
                     DTDSAYDVYQWRNGDLSLVSTGKSGSDGALYMGNDRTGRNVYFVTRDNLTWQDIDVVA
                     DVYTARVGGGIPQPPPPQVCGVLADACHGPGPAPAPVPPRTTDDGGGGNATPARKTLN
                     VRRPSRRALRRAARRGVLALRVRSSAPGVISVKAKGRVKGKRRSLGTTRKRLAEAGSV
                     RIKLRLAPRGRRQLRRAGRLRMAITADMAGARAKTVRVVLKKRGRR"
     gene            9904..13479
                     /locus_tag="VHF88_02460"
     CDS             9904..13479
                     /locus_tag="VHF88_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160661.1"
                     /translation="MATLLLAVVVAATGAPQARGDYGDDYGVAAINDGPGPLEDAPAI
                     PGRHAFWAGACDLSAAPAPGPAPIPGGVGNRPAEILAPNGDMLARQGDWMDGSPFPPE
                     FTTEYPPGSGRRWLDFNQNGIPESAPTNDSPMVKSQVFVPAPPTPDHCIDWGAMTLYN
                     DQPGIWQSFPWGGDWGSGDFGQNEPGCDASNPCSFAPSWRLPAVTQAGAHPDGTTLFA
                     WNRNTEGRGTNIGAVDGSVDNIQVDLPPGFVGNPQAVPQCTAEQFAVKPLQCPPETQI
                     GILRLHIQAVSFGGANLGAHGYDTTYPVYNLEPRPGRVAEIGFAYASGENAVTVRLTG
                     KARTNGDFGVTAYAGQIPSALIAIAQSFTLWGVPWEAENDLWRTKLGHRENNPCTQQP
                     GTPSANHYIPPGGLTVLSSGEDCQAHYQPRWGPIKPFLTNETDCNPAPTVTLTTDSFQ
                     HPGRFTAEGDPDLSDGNWKRYQSISPPVTGCASLGFAPDIDFQPTSSQADDASGLNVG
                     LSIPQNNDARNGAGAPLAPPAQGASQAAVDNYVEAATDYWESPAGRATAHLKDTVVTL
                     PKGMSVNPSAAAGLQACSDAQVGLRQLGNPPLFNNGDPFNKDGGADGAECPDGSKIGT
                     VQVQTPLLAEKLTGDVVLGEPKSTNPQSGEMFRLFLVVRNHERGLIAKIYGSSTADPN
                     DGQLTTRFLNNPELPFDRLDLSIKGGDRGLLGLPQRCGGHGWASTFTPWSSGANVDDS
                     GGFALSRNCGFGFSPALDAGMSTQAARGRGAFSFTFSRTDGQQWLRGLTAKLPTGLLA
                     SVRDVPLCDGAQASQGNCPASSRIGWVDAAAGSGTPFHLERKGDIYLTEPYKGAPYGL
                     MVRVPVEAGPFRGAMALSPIVVRQALHVDRRTAQVTAISDPFPHIHHGIPLRVRQVTA
                     VVDRPGFMLNPSDCSPKQIVADFRATHGAVSSSTEAFRASGCSKLRFKPRLTMRLTGR
                     KQTRTGKHPGVRAQVRQRGVSEAGIERAVVRLPKSLALDPDNAQALCEFEDGTKPDLE
                     RHCPKGSIVGRARAVSPLLKRPLRGNVYFVKNVRRDADTGNEIRTLPMIIVALRGEIA
                     INLRGESSTTKSGKLVNTFANVPDAPVSRFNLNIKGGRNGILAVTRTRRARINICRGK
                     QIAEADMDGHNGKAHDRNVRIKTPCKGKKKTKRKGSKKRSNKKRN"
     gene            13503..14003
                     /locus_tag="VHF88_02465"
     CDS             13503..14003
                     /locus_tag="VHF88_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160662.1"
                     /translation="MGFSKFHGGGAGLSAIAASAVLALGAVACGSDDDADDGDAGAGA
                     AKAADTGAKRAAESGSGPRAAYAKFTDSIYSMDYEAACAQMTAKHRREVVGDGDCANR
                     LRTLFRGRPPRGERPKVVAMKVDGNRATLRTRSEDSFRPYPIDFVKRGGEWKVDSSLS
                     APGGGS"
     gene            complement(14005..14574)
                     /locus_tag="VHF88_02470"
     CDS             complement(14005..14574)
                     /locus_tag="VHF88_02470"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX2160663.1"
                     /translation="MTKLLRRPAATVALGLAIGLAGCSGGDDDEGGDGSRDVPADSVA
                     VVGDRQISRDAFDDRLAAVRRSQSGSAGLSGQQLRQQAMTTLLQEAWLEREAEELGAT
                     VSDAEVRRRVGQARAQFPRRRAYRRFLGGQTERDLMFQIRVQLLGEAIEAKLREQGKS
                     RRDVFRRLQTRWSGETACRDELKVPGCEG"
     gene            complement(14659..15357)
                     /locus_tag="VHF88_02475"
     CDS             complement(14659..15357)
                     /locus_tag="VHF88_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160664.1"
                     /translation="MAATAPGYEEFDDCPGTDVDPTLQICVVSIVPDGHLKMGTKNTP
                     ITDPIELVGSLGPSGQMVVGKFDGGRQKVPGGLVGLTGLDWLTWIYPLSLLNVYAEAE
                     LAGTPGNPLVVPFPLPLKVKLDNPLLAPTCYIGSDSNPIQLNLIGSPEPTLTPDPNLP
                     GVIRLTDGVLTDHTFSVPAASGCDLLGLGLIDVLVNAQAGLPSPSGNNEAVQEVHGAL
                     AALPAIYPPDGTEQ"
     gene            complement(15561..16154)
                     /locus_tag="VHF88_02480"
     CDS             complement(15561..16154)
                     /locus_tag="VHF88_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160665.1"
                     /translation="MTLSFVACGDDESPGAAGTATTATAGERTDTDAPAEGHAEREAP
                     RTGGRSASDAIDAGSGPPSGSTAKDDGRPVPTEARRVDAVVTGMYDDMTDGDAAGVCA
                     AMSKVVRRQIAQNVVGGSTQTPEDRTCEESFSKFLDAASGSGLLEQTLDADVVDVDVD
                     GRQATATVSLSGKAGEVRLVKEEGEWRFGASPLGGSR"
     gene            complement(16284..17024)
                     /locus_tag="VHF88_02485"
     CDS             complement(16284..17024)
                     /locus_tag="VHF88_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160666.1"
                     /translation="MTPATARRGDGGSEPASGLDFLSRLLSDRALLRDWLNHCPTEVR
                     QPFTRATLLVAGLQTDSGWTVSLVLDALRCDKRKAKQVEDLRPHHLEHCVAFLAWLLS
                     LTVAAPEDRFLKRATITAMSKLYWDSGCDLEACRAAVERPLTPSDPTTPLDLLALGAI
                     DRAFAAAAAPSGAAAPGSTPEREHIGVASLDALLRYSNDEDDCSLAVSHALDLIAHIS
                     SCGLCTASYVERAKALGKEPAAAVLAPG"
     gene            17134..>17969
                     /locus_tag="VHF88_02490"
     CDS             17134..>17969
                     /locus_tag="VHF88_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160667.1"
                     /translation="MTSYVRSCSYLVASLHSETPKARPTMVLLQRGLGVRSPGGPIEA
                     PLIGREAALEEFRETLTVEGAALLCFYGEHGVGISRLLKEIAREARERGCATAHLDLA
                     ECRTPLQPGEGVVGQMSPFSRWARMRHFTFVQEELARHWPLVARELPFAAGGRALQPA
                     RTALPGLVHVSAMPALGYVWGHTGRRAMRLVARRNLERRLQAGSGLQVRLAEGRMSAD
                     RLLELAPLALALDLDDLTRRGRGFMRLVVEREGERSELEQPVGRHASLGQPDLQPGAG
                     LQP"
BASE COUNT         2727 a         6402 c         6103 g         2737 t
ORIGIN      
        1 tggcggtgcg cggcaagcca gccgaggcgc tgtcgacggt cgacgacctg ccgtacgttg
       61 agagcgtgaa cgggattggc gtcgcggcgg atggcagcgt ctacgtcgcg aagagccgat
      121 cgctgagctc gtacgacccc ggctcgggct tcgagcgctg ccgttcgccg cgtgggctgc
      181 gagcgtgcca ggggttgatg gtcctcgcct ccggcgggcc ccggacggcc gcgctcgaag
      241 gcgtcgtgcg cgtcggcgag acgactgcat cgctggaggg gaaggttcag ccccgcggtg
      301 ctgcccgcta ccggttccag gtctccgacg gtggccgctg gcgcgatgtg ggcaagccgc
      361 gctacgtctc cggcccccgc gaggtgcgcg tctcgacgct ggcgcgcggg ctgcgcccgg
      421 gcgccctgta ccgcgcgcgg ctgctgctcg cgacccgcga ccgtgacggt gaggaggacc
      481 gcgtggcctc gaacatcgcc tttctcgtca ccgtcaggcc cgaggcgttc gcgaccgccc
      541 gtccggccag ggcgttcgcg ccgctcgtcc agctccattc ggacgagcgc tccttcccga
      601 tctccgcgag ccggttcctc cagcgggcga ccgtgaagtg gaaggacggc cactgcatcg
      661 gcctgatcaa cgtcgcgact ggccgaatcg cgagccgcaa gacacccggc gccgcgcgcg
      721 ttcggccctc gaggctcggc cgcgccgctc gtccgtaccg ccatcgggcc ttcgaccgca
      781 agtgcgagcg ccgccgcccg ccggtctact cggcgatcga tctcactcgg ccgtttcacc
      841 ggggccggcg ggcggcgggc ctaccgcgcg acagcggctt ctacctcgac ctcctgagcg
      901 actcctacga cggcgatccg cgcttcgcgc ggcgcgggga gcagcgcctt ctcgtaggcg
      961 cgtccgccta ctacgagcgc cgccgggccg tcgtcgaggg tcgtcctggc atccgcctgg
     1021 cctactggct gctctacggg tccagcaggc tgccgcgggt ccgcggtgag cccatcggcc
     1081 agcacgaggg agactgggag cgcatagacg tacttgcgcg cccgggcagc cgcagacacc
     1141 gctggctgcc gcgcgcggtt cgctttcacg ttggcgatcg ccaccgcacg ctcccgtggc
     1201 gcgacgttct acggcagcgc agtcatccgg tcgtgtttgc cgccctcggc agccaccgcc
     1261 cttacccgca cgccggccgt tacgagctca gcgcacgcgt cgccggccgt cgcgcccggt
     1321 tgctcgacga gacggccgcc tgcgccaggt gcccgcaatg ggagacgtgg aagcggctcg
     1381 gccccgtccg tcgccagccc tggtacggct tcggcggcgg ctggggcctc gccttcaggc
     1441 accacgacac cagcggcccg ctggggcccc gctgagccgg gccccgaaga tgcggtctat
     1501 cacgggacgt cgtgttcgta ttccaccggc gagccttcgg ggagccagct gccttcgatc
     1561 cgcaccccat tcgggcacgt gtatgaggtg aagaaccggt actggtgagt gcctgtggtc
     1621 tcagacacca ggagcggcgc attgcagtgt gtgccaggtc cgtctggatt gccttcagca
     1681 tccaagcaga aatctacatc catgagaacc ctctccagct gcgattctct aacgttggtg
     1741 attcgccatt cgatttcact ctgtggctct gagacgccat tgcatcggac tcgcgtgcag
     1801 tcgcctccgt cactgtggtc gtccataaaa gccagtgcca caaagccagt tccgttttca
     1861 tctaactcta atagaaattc gtcctcgcag ttgctgatga tgaacccgaa gatgctcatg
     1921 tgcgattcgc cgtgcagctc caaggggcaa taattgataa tcagggagca gtggtcgctg
     1981 ccttccgcag tgacttccac cggctcttgg ctagatgcgt aggacgtagg caaggcgaag
     2041 gcgacgatcg cgaacgccaa tagcagcatg actttgtact tgcgcgacat agcggtgacc
     2101 tccaacggtc tattggtgcg ttacttcaag cggtgctcct tcgccctccc aggtgccttc
     2161 cagacggacg ccgctggggc acgtgtaggc ggccgatagc gcgtagtggt gtaactccgg
     2221 gccctccgtg aggctgaccg gcgcgttgca gtgagtgccc gtgccgctgg gattgccctg
     2281 cgcgtcgagg cagaagttca agttcgccag gccggtgttg ggccaggttt cctcgatagc
     2341 ggtcatgtcc cattcgactt cgtccgcgct ctcgccgatg ccgttgcagg gcgcacgagt
     2401 acagtcgccg ccgtcggagt gatcgaggag cacggcgttt acgacgtgtc cgctgccgtc
     2461 ggcgccgatg tgcgtagtga attcctcctc gcagttgctc acgatgaatc cgaatatcga
     2521 gaggtgcgat tcgccgtgca catccaggac gcacgggtcg gtctcgagct gacagtcgcc
     2581 gcgatcgact tcgacgggct cctgaccggg agggaccgcg tgctcgaatt cgacggacgt
     2641 gccttcggcg gcccactcgc tttcgaaccg cccgccgttg ggacacgtga agcccatcga
     2701 gaaggcgtac tggtgcgtgc ctgtctcggt gatggtgatc ggcgccgtgc agtgcatgcc
     2761 ggttccattc ggattgttcg aggcgtcgat gcagaaggta cggtccatcg tgacctggtt
     2821 gactgccgtt tcgcgagtgt tggcgatgtc ccattcgact tcgctgggac tctctccgac
     2881 tccgttgcac gggatgcgcg tgcagacacc actgttcggg tgatcgatca gatcgacgtc
     2941 ggtgacatgt cccgtgccgt cttctcccag ctcggcgacg aagtggctct cgcagctgct
     3001 gaccaggaat cccatgaccg agagatgcga ttcaccgtgg gcctcgacaa tgcatgggtt
     3061 cgcggcgatg tcgcagtgtg gcccgtcttc atgcgacagt tcgaccggct cgtgggcagc
     3121 cgaaggcgca gccacggcta ggaggcatgc gaccaggccc aagagcagcc ctcgagatgc
     3181 ttgctgccta cggcgcatcg tgtgcgactt cgatcggcgt gccttcgact gcccagtcgc
     3241 cttcgaggcg gatgccgttc gggcaggtgt aggccatcga gagcgagtag tggtgggttc
     3301 cctgggtctc cctcagggac atcggcgtgc tgcagtgcgt ccctgtggcg ttggggcttc
     3361 cttgggcgtc gatgcagagg ttgcggtcga aggtcgtctc acccggggcg gtttcgcgtg
     3421 cgttgacgat gtcccattcg acttcggcgc tgctctcgcc gacgccgttg cacgggacac
     3481 gcgggcagtc gccaccgtcg gagtggtcga agatcgtggc gcttgtcgtg tggcccttgc
     3541 cgtcttcgcc gagttgagcg gtgaattcat cctcgcaaat gaagatgatg aaaccgaaac
     3601 tcgagatgtg ccattcgccg tgcgcggtca gctggcaggg attggtgctg atgtcgcagt
     3661 gcgctccgcc ttcgtgctcg agtgcgaccg gctcttgagc ccacgcgctc gacgcgaggg
     3721 tgatcgaggc gagggcagct gcgcagagcg gcgggatctt gacggctacg gacacttgga
     3781 ctcccgtgag cttgcttgca aaagcagaga ggccccgctg agcggggcct ctctgtacta
     3841 ccatcgatca tctaagaccg attactgcgc cacatcgtgt tcgatttcga tgggcgtgcc
     3901 ttcggcttcc caattacctt cgactcgaac accgttgggg cagatgaacc cggtcgagaa
     3961 cgtgtagtgg tgcgttcccg tggtctcccg aacggtgatc ggcgcgttgc agtgcgtgcc
     4021 ggtcccgttg gggttcccgg cggcatcgag acagaagttc acgtccatga tgccgacgtc
     4081 agtgcctgtt tctcgggtgt tggtgatgtc ccattcgact tcacccgatc cctcgcccac
     4141 gccgttgcac ggaacacgcg tgcagtcgcc gccatccgag tggtcgacga gcgaggcgtt
     4201 ggtgatgtgc ccggtgccat cttcgccgat agaggccgtg aattcatcct cgcagttgct
     4261 gacgatgaat ccgaacacgg agagatggga ttccccgtgg gcgacgacat cacacggatt
     4321 ggcgctgatg tcgcagtgcg ctccgccttc gtgctcgact tcgaccggca cctgcgccga
     4381 tgcggctgag gctgcgaggg ccatggcggc gaacgccatg gcgcacagca gcgctagctt
     4441 gcgtgcgata cgcattgttt gaatctcctt gttggtccgg cggacctgcc gccgggttcg
     4501 gcgccgacct cgacccctcc ggccgggtct cgacgcaccc tacgaagcgc tcacgcggta
     4561 ggcgcggcgc gtgcttgccg cctgtcgggc ggctgccgct gcaagcggca ggcgttgtcg
     4621 gtcgtgcggt ccctcctttc cgctttcgtg gctccctacc ctccttgaag ccatctcaat
     4681 cgagagaagc atgtgtctct ctcccgacgg ccggcaaggt acatcggcgt ttcagagatg
     4741 tcaagagaag cgacgggtcc ctcttgacag ttcggatacg actgtgttct catcatcgcc
     4801 acgcctacgg gaggatccgt ggcgcgaggg tggagccgtc tagggaggag ctcgagtgcg
     4861 gaagaggact gcaaacgacc gaggtcgtgg ccgccggtcg acggtgggcc tgggagcgac
     4921 gatcgtcgtc gcggccgtgc tgggccttgc cgcatcggcg ctcgcgaccc tctatgtggg
     4981 ttcgttcttc ggcaacaccg gcaacaccgg ggggctgttc aacacgccca acggcgtggg
     5041 cgtgcacaac gccagcggca atgcctacgt cgcggaccgc ttgaaccacc gcgtgcagca
     5101 gttcaacgcc tcgggacagt tcgttcgtgc ctggggccgc gacgtcgtca gcgccggccc
     5161 gggcaacgcc gacgagcagc agctggcgac ggtcaacgcc acggccggga cctacacgct
     5221 cacctacagc gggcagacga ccgtgccgat cgccttcgac gcgccggcct cagccgtgca
     5281 gaccgcactc gaggatctct cgaacctcgc accgggagac gtgacggtct ccgggggccc
     5341 tggcggcgtc ggcggtgcga gcccctacgt gatccagttc ggaggcacgc tggccaacgt
     5401 gaacgtcgcg cagatcaccg cttcgagcgc cggcctcacc ggaactgcga cgaccgccac
     5461 cgcgaacgag ggcgcgaccg gcttcgagat ctgcgtcgcc gccaacggcg acacctgcaa
     5521 ggcgggcacg acgaccggcg gcatcggcgg cgccttcaac aaccctctcg gcgtcgcggt
     5581 caaccagggg accggcgacg tctacgtgag cgacggcagc gcgagccacc gacgcgtcca
     5641 gcagttcacg tcgaccggtc agttcatccg cgcctggggc caggacgtca tcgccggcac
     5701 ggcgcccaac tccaacggca ccggcttcga gatctgtgac acgacggctg cctcggcgaa
     5761 cttccacaat cagtgcaagg cgggcctctc gcagagcacc ggcggggcct tcgggacgat
     5821 ctccgcggcc aacggcggct acctcgcggt cgtcccggcc ggcgccccga acgccgggaa
     5881 cgtcgtcgtc gccgaccccg ggaacaaccg tgtgcaggag ttcacggcgt ccggcgcctt
     5941 cgtccgcgcc ttcggtggcg acgtcgagaa cccgcccgga ggtagtgcct tcgaggtctg
     6001 caccatcgcc gccaactgca aggccggtgc cccgggcacc gccgtcggcc agttcgacgc
     6061 cagcttcccc cggcgcgtgg cggccgacgc gaccggcgcc atctacaccg tcgagagcag
     6121 cgtggcggcc cgccgcgtcc agaagttcac cccccaggcc cttccgcccg gcctcgcgcc
     6181 atcgctgttc gccgacgcga tcgtcggaac cagcggcgag ttcccgctcg agccgctcga
     6241 tgtcgccgtc gatcccgtca gcggccgcgt cttcgtcacg aagacctatg gggctggcac
     6301 gttcacctgc cccgacggct ccccgtccat cccgaccgag gtgcgcgtcg tcgagctgag
     6361 ctcatcgggc acgcagctcg acagccatgc cgggtgcgcg ggcttcacgt tctcgaacgt
     6421 cctcgggctg gccttccggc catcagcggg agggggcgac agcgagcggc tctacatctc
     6481 ctcgaccaac ggcggccatc gggtgtacgt cctcgaccaa gacggcatca cggccgcgat
     6541 cgccaacgtc aatccggcga ccgacatcac ctcgacgacg gcgctgctca gcgccacggt
     6601 gaacccgaac agcgtcgaca acagcttcgc gccgaccgtc tggcggatcg agtactcgcg
     6661 caacggcgcc acctggacga cggccaacag cggctcgctg cccgcgggca cgacgcccga
     6721 gacggtcaca gccgaggtct ccaaccttct gccgaacacc acctaccagg tgcgtgtcgt
     6781 cacccgcaag ccgctcggca acccggaggt cgtgtcgccc gagctgacgt tccgcaccga
     6841 tgcggtcagg ccggagatcg gcacggtggg agccgacacg atcacctcga cctccgcgcg
     6901 gctcaccggt cagatcgtgc cgcacagcac gccgacgacg taccggttcg agtggggcca
     6961 ggggagcttc agcaacgtga cgccggtccc cgatgggtcg gccggcgccg gcccgaccgc
     7021 cgtgttcgtg ttccagcagc tggccggcct ccagcccggc acgacgtacc agttccgcct
     7081 cgtggcgacc tcccagaccg agggcgccac ttcgagcgag acgcggacgt tcaccaccct
     7141 gtcgccgggg gcgcgggcag acgtggacgc gcgtggctac gagctcgtct cgccgcccga
     7201 caaggtcggc ggtacgggcg tcggcgagtg gtaccagggc ccgggcagca tgggcagcgc
     7261 aggcctcgcg gcttacgagg gcgagcgctt tgccgccaac ggcgcgttcg gctccacgct
     7321 gatggacgga gcccacgcct gggcaaacga ctgggcgttc gcccagcgca tcggcgacgg
     7381 ggtcggctgg cagagccact cgccgctgac gcacccgaac taccgcgcga cgctagcggg
     7441 caagctgaat ctcgtctcga cgacgcccgc cctgtcgcgc ttcttctggc acagcccgca
     7501 gggcaccccg gcgatgttcc gcgagatcgc gaacgagcct ggctggggca cccctgccgg
     7561 actgttcagc gactggggcg caccgacgcg ctgggaggtg ttcggacccg ccgatcccct
     7621 cgacgaggac aatcccgaca acgaccagat gctgggttcg acctcgaatc tgatctgggg
     7681 agtcggcttc tccgacgacg gctcgctggc ggcggccgtg actgagctca acgccggcaa
     7741 ggtggtaggg ctcgggggtc cgagcgaccc gaccactccg cgcccgatgg cgccgtccgg
     7801 cgacctcgtc gagggccgct cgatctacct cgcggacacc tcgaacacgc tccgcgacgc
     7861 cttcgacccc gctgtccagc ggacgctggc caatgtctgc accggcgccg gcgcgacccg
     7921 gaccgaggtc ccggccgtcg acctgaccgg caacctcacc gccgaggcct gcacggacga
     7981 ccccggcgac gacctgatct ccaattacgg ggcgacgctt cgcggcggcc cgggcgcgaa
     8041 cgacccggta cgagtcgacg acgtcgtcaa cgccgacggc tcgcgcgtct tcttcatgag
     8101 ccccgatccg gcggccccca gcgtcccgaa cgggctcacc tcgttctgct cgaccgtggg
     8161 cggcacctgt cccacgcagc tctacgtgcg tcagcagagc gcagacggga gcaacgtcac
     8221 cacgcgctgg atctcgaagg ccgaggacgg cctcttcggc acccaggact cgacgctcac
     8281 cggcactgtg cgcttcgagg gcgcgagccg cgacggggac aaggtcttct tccggaccaa
     8341 ctcgccgctc accgcagacg atccgaacgg gggctgtggc gcgccctgca ccaccggcaa
     8401 cgcgtcgccc aactcgtggg acctctacct gtacgacctg ccggacgcgc ccggcgcgga
     8461 cccgggcgac ggggacctga cgcgcatctc ggccggaccc ggcggcgacg gcgactgcaa
     8521 cagcccgctc ggcggggacg gcgatgtcgg cgcgctgcgc ttcgcctccg aggacggcgg
     8581 ccgcgtgtac ttcacctgcg ccgcgccgct ggccggcgtg ggcgcgccgt ccaacggcac
     8641 ggtgacgacg ccgggaggca gccagacgac gcccggccag accaacctct acgtctacga
     8701 caccagccgc gacgccgacg accgctggcg gttcgtcgcc cggatcccgc gctcgaccga
     8761 caggacgatc gagacgtgcg cgagcaccgg ccaggaccgc cgctcgccgc tcgcggcgcc
     8821 cgctcaggcg ccgtcgacct tcgtgatcga ggcggctgct gccgacgcga actgcctgag
     8881 cggcacgtcc gacggcgcgt tcgtcacctt cttcaccacc ggccggctga ccgccgacga
     8941 cccgacctcc cccgttacag gcgacgtcta cggctatgac gccgcggccg acgacctggt
     9001 ccgcatcacg gctccgcagg gcggtgtcgg cgagacatat ccgtgcgcgc ccggcaacag
     9061 cgccgtcgcc tgcttcggcg acagtggcgt gggcgtccag cccggcatcg gatcgggcac
     9121 gtcgaccgcg ccgctcggcg tcgcgaccga cccgctggtc ggtggcgacc gggtcgcctt
     9181 cctgcagtcg cgcagccggc tcgtcccgga ggacaccgac agcgcctacg acgtctacca
     9241 gtggcgcaac ggtgacctgt cgctggtctc gaccgggaag agcggcagcg acggcgcgct
     9301 ctacatgggc aacgaccgca ccgggcgcaa cgtgtacttc gtcacgcgcg acaacctcac
     9361 ctggcaggac atcgacgtcg tcgcggacgt ctacaccgca cgcgtcggcg gcggcatccc
     9421 gcagccgccg ccaccccagg tctgcggcgt gctcgctgat gcgtgccacg gccccggccc
     9481 cgcccccgcc ccggtgccgc cgcggacgac cgacgacggc ggtggcggca acgccacccc
     9541 ggcccggaag acgctcaacg ttcgccggcc ctcgcgcagg gcgctgagga gggcggctcg
     9601 ccgcggcgtg ctggcgctca gggtccgcag ctccgccccg ggcgtcatct ccgtcaaggc
     9661 caagggccgc gtgaagggca agcggcgatc gctcggcacg acgcgcaagc gcctcgccga
     9721 ggccggcagc gtgaggatca agctgaggct cgcgccgcgc ggacggcgcc agctacgaag
     9781 ggccggcagg ctgcgcatgg cgatcaccgc cgatatggcg ggcgcccgcg cgaaaaccgt
     9841 gagggtcgta ttgaagaaga gagggcgacg gtgaatccag tggttactcc cctgaggatg
     9901 ttgatggcca ccctgctgct ggcagtggtg gtggcggcca ctggagcgcc gcaggcacgc
     9961 ggcgactacg gcgacgacta cggcgtcgcg gctatcaacg acggccccgg cccgctcgag
    10021 gacgcgcctg cgatcccggg caggcatgcc ttctgggccg gcgcctgcga cctttctgcg
    10081 gcacccgctc ccgggcctgc gccgatcccc ggtggcgtcg gcaaccggcc cgccgagatc
    10141 ctggccccca atggcgacat gctagcccgc cagggggact ggatggacgg ctcccccttc
    10201 ccgcccgagt tcacgacgga gtacccgccc ggtagcggtc gccggtggct cgacttcaac
    10261 cagaacggca tcccggagag cgctccgacc aacgactcgc cgatggtgaa gtcgcaggtc
    10321 ttcgtcccgg cgccgccgac gcccgaccac tgcatcgact ggggcgcgat gacgctctac
    10381 aacgaccagc ccggcatctg gcagagcttc ccgtggggcg gcgactgggg cagcggcgac
    10441 ttcgggcaga acgagcccgg ctgcgacgcc tcgaacccgt gcagctttgc gccgagctgg
    10501 cggctgccgg ccgtcacgca ggccggcgcc catcccgacg gcacgacgct gttcgcctgg
    10561 aaccgcaaca ccgagggcag gggcacgaac atcggcgcgg tcgacggatc ggtggacaac
    10621 atccaggtcg acctgccgcc gggcttcgtc ggcaacccgc aggccgtgcc gcagtgcacc
    10681 gccgagcagt tcgcggtcaa gccgttgcag tgcccgccag agacgcagat cggcatcctg
    10741 cggctgcaca tccaggccgt cagcttcggc ggcgcgaacc tcggcgcgca cggctatgac
    10801 acgacctacc ccgtctacaa cctcgagccg cgccccggcc gcgtcgccga gatcgggttc
    10861 gcatacgcaa gcggcgagaa cgccgtcacc gtgcggctca ccggcaaggc gcgcaccaac
    10921 ggggacttcg gcgtcaccgc ctacgctggc cagatcccgt cggcgctgat cgcgatcgcc
    10981 cagtcgttca cgctctgggg cgttccgtgg gaggcggaga acgacctctg gcgcacgaag
    11041 ctcggacacc gcgagaacaa cccctgcacc cagcagccgg gcactccctc cgccaaccac
    11101 tacatcccgc caggcggcct cacggtcctc tcgagcggcg aggattgcca agctcactac
    11161 cagccgcgtt ggggcccgat caagccgttc ctcaccaacg agacggactg caacccggcg
    11221 ccgaccgtca cgctgacgac ggattccttc cagcaccccg gccgcttcac cgcggagggc
    11281 gaccccgacc tgtccgacgg gaactggaag cgctaccagt cgatctcacc gcctgtgacc
    11341 ggctgcgcat cgctcgggtt cgcgcccgac atcgacttcc agcccacgag ctcgcaggcc
    11401 gacgacgcct ccggcttgaa cgtcggtctc tcgatcccgc agaacaacga cgcgcgcaac
    11461 ggcgccggcg ccccgctcgc gccgcccgcc cagggcgcct cgcaggcggc tgtcgacaac
    11521 tacgtggagg ccgccaccga ctactgggag tcgccggccg gtcgcgcgac agcgcacctc
    11581 aaggacaccg tcgtgaccct gcccaagggc atgtcggtca acccctcggc cgccgcgggc
    11641 ctccaggcct gcagcgacgc gcaggtcggc ctgcgccagc tcggcaaccc accgctgttc
    11701 aacaacggcg acccgttcaa caaggacggc ggcgccgacg gggcggagtg ccccgacggg
    11761 tcgaagatcg gaaccgtgca ggtgcagacg ccgctgctcg ccgagaagct caccggcgac
    11821 gttgtgctcg gcgagccgaa gtccaccaat ccgcagtccg gcgagatgtt ccggctcttc
    11881 ctcgtggtgc gcaaccacga gcgcgggctg atcgccaaga tctacggctc cagcaccgcg
    11941 gatcccaacg acggccagct cacgacgcgg ttcctcaaca accccgagct gccgttcgac
    12001 cggctcgacc tgagcatcaa gggcggcgac cgtggcctgc tcggcctgcc gcagcgctgc
    12061 ggtggccacg gctgggcctc gacgttcacg ccatggtcca gtggcgccaa cgtcgacgac
    12121 agcggcgggt tcgccctcag ccgcaactgc ggcttcggct tctcgccggc gctcgacgcc
    12181 gggatgtcga cccaggccgc gcgcggccga ggcgcgttct cgttcacctt ctcgcgcacc
    12241 gacggccagc agtggctgcg cggcctgacg gcgaagctgc cgaccggcct gctcgcgagc
    12301 gtccgcgacg tgccgctctg cgacggcgca caggcttcgc agggcaactg cccggccagc
    12361 tcgcggatcg gctgggtcga cgccgcggcc ggctctggca ccccgttcca cctcgagcgc
    12421 aagggcgaca tctacctcac cgagccctac aagggcgctc cctacggcct gatggtgagg
    12481 gtcccggtcg aggccggccc cttccgcggc gcgatggcgc tgtcgccgat cgtcgtccgt
    12541 caggcgctgc acgtcgaccg caggaccgct caggtgactg ccatctccga tccgttcccg
    12601 cacatccatc acggcatccc gctccgggtg cgccaggtga ccgcggtcgt cgatcggcca
    12661 ggattcatgc tcaacccgtc cgactgctcg ccaaagcaga tcgtggcgga cttcagggcc
    12721 acccacggcg cggtgtcgag ctcgacagag gcgttccgcg cctccggctg ctcgaagctg
    12781 cgcttcaagc cgcgcctgac gatgcgcctg acgggcagga agcagactcg caccggcaag
    12841 cacccgggcg tcagggcgca ggtgaggcag aggggcgtct ccgaggcggg catcgagcgc
    12901 gccgtcgtac ggctgccgaa gtcgctcgcg ctggacccgg acaacgccca ggcgctgtgc
    12961 gagttcgagg acggcacgaa gcccgacctc gagcgccact gcccgaaggg gtcgatcgtc
    13021 ggtcgtgcgc gcgccgtgtc gccgctgctg aagcgcccgc tgaggggcaa cgtctacttc
    13081 gtcaagaacg tccgacgcga tgccgacacg ggcaacgaga tccgcacgct gccgatgatc
    13141 atcgtcgcgc tgcgtgggga gatcgcgatc aacctgcgcg gcgagagctc gacgaccaag
    13201 agcggcaagc tggtcaacac ctttgccaac gtgcccgacg cccccgtctc gaggttcaac
    13261 ctgaacatca agggcggccg caacggcatc ctcgcggtga ccaggacgcg tcgcgcgcgg
    13321 atcaacatct gcaggggcaa gcagatcgcg gaggcggaca tggacggcca caacggcaag
    13381 gcccatgacc gcaacgtccg gatcaagacc ccctgcaagg gcaagaagaa gacgaagagg
    13441 aagggctcga agaagaggtc caacaagaag cgcaactgac tgactgggag ggagttggtt
    13501 cgatggggtt ctcgaagttc catggtggag gcgccggtct gagcgcgatc gccgcgagcg
    13561 ccgtgcttgc gctcggcgcc gtcgcctgcg gctcggacga cgacgcggac gacggcgacg
    13621 ccggcgcggg ggcggcaaag gccgccgaca cgggtgccaa gcgcgccgcg gagagcggca
    13681 gcggcccgcg cgccgcctac gcgaagttca ccgacagcat ctactcgatg gactacgagg
    13741 cggcctgcgc gcagatgacc gccaagcacc gccgcgaggt cgtcggcgac ggcgactgcg
    13801 ccaaccgcct gcgcaccctc ttccgcggcc gcccgccgcg cggcgagcgg cccaaggtcg
    13861 tggcgatgaa ggtcgacggc aaccgggcga cgctgcgcac gcgctccgag gacagcttca
    13921 ggccctaccc gatcgacttc gtcaagcggg gcggcgagtg gaaggtcgac agcagcctct
    13981 ccgcacccgg cgggggctcc tgagctagcc ctcgcagccg ggaaccttca gctcgtctcg
    14041 gcacgcggtc tcgccgctcc agcgggtctg cagccgccgg aagacgtcgc ggcggctctt
    14101 gccctgctcg cgcagcttcg cctcgatcgc ctcgccgagc agctgcaccc ggatctggaa
    14161 catcaggtcc cgctccgtct ggccgccgag gaaacgccgg taggcgcgcc gcctcgggaa
    14221 ctgggcgcgc gcctgcccga cgcgccggcg gacctccgcg tcgctcacgg tcgctcccag
    14281 ctcctcggcc tcgcgctcga gccaggcctc ctggagcagg gtggtcatag cctgctgacg
    14341 cagctgctgc ccgctcagcc cggccgatcc gctctggctg cggcgaaccg ccgcgagccg
    14401 gtcgtcgaac gcgtcgcgcg agatctgccg gtcgccgacc acggcgaccg agtccgcggg
    14461 gacgtcgcgc gagccgtcgc cgccctcgtc gtcgtcgcct cccgaacagc cggcgaggcc
    14521 gatggcgagt cccagcgcga cggtggcggc ggggcgtcgc agcagcttag tcatggacgc
    14581 caccgtacac gagctggcgg aggcccctag gggcctccgc caggtgtcgc gatgctgctg
    14641 ccttgacgcg gtcgagcgct actgctcggt cccgtcaggg gggtagatcg cgggcagtgc
    14701 cgcgagcgcc ccatggactt cctggaccgc ttcgttgttg ccggacggcg aaggcagtcc
    14761 ggcctgcgcg ttgacgagca cgtcgatcag gccgaggccc agcaggtcgc agccgctggc
    14821 cgccggcacc gagaacgtgt ggtcggtcag gacgccgtcg gtcagccgga tcacgcccgg
    14881 caggttcggg tcgggcgtca gcgtcggctc gggcgagccg atcaggttca gctggatcgg
    14941 gttgctgtcc gagccgatgt agcacgtggg ggcgagcagc gggttgtcca gcttgacctt
    15001 cagcggcagc gggaacggga ccacgagcgg gttgcccggg gtgccggcca gctcagcttc
    15061 ggcgtagacg ttcagcaggc tgagcgggta gatccaggtg agccagtcga gcccggtcag
    15121 gccgacgagc cctccgggaa ccttctgacg accgccgtcg aacttgccga cgaccatctg
    15181 gccgctcggc ccgagcgatc cgaccagctc gatcgggtcg gtgatcggcg tgttcttggt
    15241 ccccatcttc aggtgaccgt cgggtacgat cgagaccacg cagatctgaa gcgtggggtc
    15301 gacgtcggtg cccgggcagt cgtcgaactc ctcgtagccc ggcgccgtgg cagccatcgc
    15361 gccggatgcc atggccagaa acgcgaccaa ggcgaacgtg agcgcggccg cgcgtttgac
    15421 agtacgcatg ccttcctcct ctttgagtga gccggctcgg gcttccctcc cccgtcggcc
    15481 ggccgcgccc ctacctggag cgcggcccga gtatatccgt gttcctagct cgaaagcaac
    15541 agggggcgaa atcggatcgg ctatcgcgac ccgccgaggg gcgacgcgcc gaagcgccac
    15601 tcgccctcct ctttgacgag ccgcacctcc ccggccttgc cggagagcga cacggtcgca
    15661 gttgcctgcc ggccgtcgac gtccacgtcg accacatcgg cgtcgagcgt ctgctcgagc
    15721 aggccgctcc cactcgcggc gtcgagaaac ttagagaagc tctcctcgca ggtgcggtcc
    15781 tcgggcgtct gcgtcgagcc gccgactacg ttctgagcga tctgccggcg cacgaccttc
    15841 gacatcgcgg cgcagacgcc ggccgcgtct ccgtcggtca tgtcgtcgta catcccggtg
    15901 actacggcgt cgacgcgccg ggcctcggtc ggcacggggc ggccgtcgtc cttggcggtg
    15961 gagccggacg gggggcccga tcccgcgtcg attgcgtcgc tcgcgctgcg gccgccggtc
    16021 cggggcgcct ctcgctcggc gtggccctct gcgggcgcgt cggtgtccgt ccgctccccg
    16081 gcggttgccg tcgtcgccgt gcccgcggcc ccaggcgact cgtcgtcgcc gcaggcgacg
    16141 aagctcagag tcatcgaggc caatgcgagt agcgtgagca cgctgcggat cgcttgcaca
    16201 gggctgtgca tctgattctc cctccagaag gtgacgccgg aagcctaacg cccgacagcc
    16261 acccgatccc ggcgccgccg ccgtcagccg ggcgcgagga cggccgcggc gggctccttg
    16321 ccgagcgcct tcgcgcgttc gacgtagctg gcggtgcaga ggccgcagct cgagatgtgc
    16381 gcgatcaggt caagggcgtg gctgacggcg aggctgcagt cgtcctcgtc gttcgagtac
    16441 cgcagcagcg cgtcgagcga agcaactccg atgtgctcgc gttcgggcgt cgaaccgggc
    16501 gcagcggccc cggacggcgc cgccgcggcg gcgaacgccc ggtcgatggc gccgagcgcg
    16561 agcagatcga gcggcgtggt cggatccgac ggcgtcagcg gccgctcgac cgccgcccgg
    16621 caggcctcga ggtcgcagcc gctgtcccag tacagcttgc tcatggcggt gatggtcgcg
    16681 cgcttgagga agcggtcctc cggcgccgcg acggtgagcg acagcagcca cgccaggaac
    16741 gccacgcagt gctccaaatg atgcggacgc aggtcctcga cctgcttcgc cttgcgcttg
    16801 tcgcagcgca gggcgtcgag caccagcgac accgtccagc cggagtcggt ctgcaggccg
    16861 gccaccagca gcgtcgcccg agtgaacggc tgccgcacct cggtcgggca gtggttcagc
    16921 cagtctcgca acagcgcccg atcgctgagc aggcggctga ggaagtcgag tcccgaggcg
    16981 ggctcggacc cgccgtcgcc tcgtctcgcg gtggcgggcg tcacctgttc ccaggtctct
    17041 cttgccgagc aaggcgcccc acacgcaaac aattcaggtt caagcggatt ctgtcaagtc
    17101 ccatatgaca catcgccggt gagcgcttgg ggcttgacga gctacgtacg tagctgttct
    17161 tatctagtcg cgtcactcca ctccgaaacc ccgaaagcaa ggccgactat ggttttgttg
    17221 caaagaggct tgggtgtacg gtcgccgggc gggccgatcg aggcgccgct gatcgggcga
    17281 gaggcggcgc tcgaggagtt tcgcgagacg ctgacggtcg aaggggccgc gctgctttgc
    17341 ttctacggcg agcacggcgt cggcatctcg cgtctgctca aggagatagc gcgggaggcg
    17401 cgcgagcggg gttgcgcgac ggcccatctc gatctcgccg agtgtcgcac gccgctgcag
    17461 ccgggcgagg gcgtcgtcgg ccagatgtcc cccttcagcc gctgggcgcg gatgcgtcac
    17521 ttcaccttcg tccaggagga gctggcgcgc cactggccgc tcgtcgcgcg cgagttgccg
    17581 ttcgcggccg gtggccgggc tctccagccg gctcgcacgg ctcttcccgg cctcgtccac
    17641 gtgtcggcga tgcctgccct ggggtacgta tggggccaca ccggccggcg ggccatgcgc
    17701 ctcgtcgcga ggcgcaatct cgagcgacgg ctgcaagccg gctccgggtt gcaggtccgg
    17761 ctggccgagg gacgcatgtc ggccgaccgg ctgctcgagc tcgctccgct cgccctcgcg
    17821 ctcgacctcg acgacctcac ccgccgcggc cggggcttca tgcggctcgt ggtcgagcgc
    17881 gagggcgagc ggagcgagct cgagcagccg gtcggccgac atgcgtccct cggccagccg
    17941 gacctgcaac ccggagccgg cttgcagcc
//
LOCUS       DATABV010000062         9155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2749078, whole genome shotgun sequence.
ACCESSION   DATABV010000062 DATABV010000000
VERSION     DATABV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2749078"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1217)
                     /locus_tag="VHF88_02495"
     CDS             complement(42..1217)
                     /locus_tag="VHF88_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2160668.1"
                     /translation="MVDFTLTDEQKDIQSMAHDFAEKEMRKVAWELDKEGTWPGEIIE
                     KAWDVGLMNTHIPAEYGGPGLDFLTGCVIEEEIGWGCSGIGTSLMCNGLATAPIVLGG
                     SEETKKKYLGMLTEAPKLASFCLTEPDAGSDVSGMKTTAVKQGDKYVINGSKCFITNG
                     SHADYYTVYAKTDKEAGHRGMSAFVVERNWDGVTVDKKEDKLGQRASNTATISFNDVE
                     VPAENLIGEENKGFKLAMMTLDRTRPGVAAMAVGIARSAFEFATEYSKERVQFGVPIA
                     MHQAIQFMIADMATKVEAARLLTWKSAALLDNGERNTLVSSHAKRFAADSAMEVTTDA
                     VQVYGGYGFIKEYPVEKLMRDAKIMQLYEGTSQIQRLVIAREVLLPRRAEAPAPAAA"
     gene            1382..1810
                     /locus_tag="VHF88_02500"
     CDS             1382..1810
                     /locus_tag="VHF88_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160669.1"
                     /translation="MAETFGLVVIVIALVAALAACASYAGSGRVYRAIGRSGLSLDEP
                     DLRPTPQPGSAAWLAEAEAELRQLLDAKSARSEARGDGAIDVDAELERLSRPQRNADP
                     ALRAEVRELVVATNERRARRGQPPLDVEAEVDRRLEDLGA"
     gene            1839..2192
                     /locus_tag="VHF88_02505"
     CDS             1839..2192
                     /locus_tag="VHF88_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160670.1"
                     /translation="MADGRRFEIDELLVRPGTYFNPQTEVLVVVDDSSSMDNEIFALE
                     EFEGADWILIADDLPVDEPQRDELLETFQATYHGGDGRQPRVLEDPESDDEGDPLGQE
                     DHERHGASIEHVDDE"
     gene            2221..3300
                     /gene="cax"
                     /locus_tag="VHF88_02510"
     CDS             2221..3300
                     /gene="cax"
                     /locus_tag="VHF88_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX2160671.1"
                     /translation="MRPVHYLAVFIPAAVALELAHAAPVLIFGAAALGVIPTAAVMGE
                     ATEQIAAKTGPGIGGFLNVTFGNAPELIIAFFALKEGLQEVVKASIVGSIVGNILLVL
                     GAAMLVGGLKREKQTFNRTAATAQSAMLLLALTALIMPALFQLIHGGGLPGVGDERVD
                     FGSDLETLSLGVALVLMGTYALGLLFSLKTHRRLFNPFDEGDDHHAGWSVRKALIALG
                     VSAVLVGLMSEILVGSIGEASDEIGLSEFFVGVFVVAIVGNAAEHWVAVLVAAKDKMD
                     LAVGIAVGSSAQIALFVAPVLVLLSFVVGPEPMALVFNGYEIGGLVFAVLIANFLTQD
                     GESNWFEGVQLLALYAVLGIVFFLA"
     gene            complement(3386..4360)
                     /locus_tag="VHF88_02515"
     CDS             complement(3386..4360)
                     /locus_tag="VHF88_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX2160672.1"
                     /translation="MILEEIAPWAALAVALLVFLWLDLHFFARGREPNFKEAVWWSVG
                     WLVLSLAAALVVWVLEGGEDAVLYTTVYLIERSLSLDNLFVFLLLFSYFGVPYEQRGR
                     LLFWGIVAALVLRGLFILIGIALIEQFHFVIYLLGVALLALAYRIFRGVDDNVDPDRN
                     LLVRLVRRFYPVSSTFDGKHWFVRKDGRRYATPLFLCLAAVVFADIAFAIDSIPAAFA
                     ITRDPFLIWMGNVFALLGLRALFVLVEGLIRRFRYLDQTIAVVLGVVGVKLLVEDFVH
                     VGPLASLAIIAVLFAIGIVASIVADRRDPDSEDKQAERAHRTEQTRSP"
     gene            complement(4388..5416)
                     /locus_tag="VHF88_02520"
     CDS             complement(4388..5416)
                     /locus_tag="VHF88_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160673.1"
                     /translation="MTIRTALRQPWTAVLLAVVAVAAPISLAGCAGGGDEGGRGGDED
                     ARALLDRAFDEPVPSADLEIDLQLDLEGLAGFEDPLRVVASGPYVRGRDALPQLDLDI
                     ALEAQGAGQAIQSGLLSTGDRVFLKFGGAFYEQPAAQVASANRRLARGGDANGSLSDL
                     GLDTREWIVDARLDGEEEVGGVTTEHVSGTLDVEAALRDINGLVRRSAGALGEAADAA
                     RPLRPREIERLARSVENPAFDVYVGEDDRIVRRVSLRVDVEVPEQDRADVGGVTGASI
                     RLAAQLGDVGGDQRIEAPRSARPLSELMSQIGSLDSIVGDERYADCLDAADPGDSAAI
                     ARCRTLLP"
     gene            5507..7078
                     /locus_tag="VHF88_02525"
     CDS             5507..7078
                     /locus_tag="VHF88_02525"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HEX2160674.1"
                     /translation="MTLRDSVLCTSATVCIGLALAVPAQAGELILVGHGKPAERVHDA
                     FVPTRGAGDLGREPARRARRSGALAAGAKLARRPARVTAPPRARAARTSRRARRATSR
                     ALALARRRGAISSARYVKYRRIVMRARAVERRLSGARAAELANAIALVDGIALRRKLT
                     AGRLPALMLILQRNTQYWPRQPFPSDRDHVMFRGSRLIFEYYAGEGLQLQPLVNFKQA
                     NLMHGACVKDTGEPCDRAGLRRLLRELVRTSTRRGGFKTWEYYFEFGGGRPPWISGMA
                     QATAIQAFARASELLNAPGYLKQARAAFGAFRKPPPIGVRTTGPFGGTHYLQYSFAPR
                     LYIINAFLQALIGLYDYAEITGDRTARALLEDAEPEAREELVANDTGDWSTYSYRGAE
                     STREYHELVREFAASMCSRLRRKVYCDTARNFLSYTTDPAELELLGPAAATKRVPTNI
                     RFWVSKLSAVQVTVTRNGRVELDKLDTFRRGNGAFEWTPRAVGTYRIRVAAKELRTGR
                     GLRSSVTGAVEAQLP"
     gene            7161..8300
                     /locus_tag="VHF88_02530"
                     /pseudo
     CDS             7161..8300
                     /locus_tag="VHF88_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
     gene            complement(8367..9146)
                     /gene="clpX"
                     /locus_tag="VHF88_02535"
                     /pseudo
     CDS             complement(8367..9146)
                     /gene="clpX"
                     /locus_tag="VHF88_02535"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
BASE COUNT         1383 a         3147 c         3222 g         1403 t
ORIGIN      
        1 ccggggggcg gcctcttcag gactcggcgt gtcgggggtc tctaagccgc agcgggcgcg
       61 ggcgcctcgg cgcgacgcgg cagcagcacc tcgcgggcga tgaccaggcg ctggatctgg
      121 gaggtgccct cgtagagctg catgatcttc gcgtcgcgca tcagcttctc gaccgggtac
      181 tccttgatga agccgtagcc accgtagacc tggacggcat cggtggtgac ctccatcgcg
      241 gagtcggccg cgaagcgctt ggcgtgcgag ctgacgagcg tgttgcgctc gccgttgtcg
      301 agcagggcgg cggacttcca ggtcagcagg cgggcggcct cgaccttggt cgccatgtcg
      361 gcgatcatga actggatcgc ctggtgcatc gcgatcggca cgccgaactg gacgcgctcc
      421 ttcgagtact cggtcgcgaa ctcgaacgcc gagcgggcga tgccgaccgc catcgccgcg
      481 acgccggggc gcgtccggtc gagcgtcatc atcgccagct tgaagccctt gttctcctcg
      541 ccgatcaggt tctcggccgg gacctcgacg tcgttgaaag agatcgtcgc cgtgttggag
      601 gcacgctgtc cgagcttgtc ctccttcttg tcgacggtga cgccgtccca gttgcgctcg
      661 acgacgaagg cggacatccc gcggtggccg gcctccttgt cggtcttcgc gtagaccgtg
      721 tagtagtcgg cgtgcgaccc gttggtgatg aagcacttcg agccgttgat gacgtacttg
      781 tcaccctgct tgacggccgt cgtcttcata ccggacacat cggagccggc gtccggctcg
      841 gtcaggcaga aggaggccag cttcggcgcc tcggtcagca tgccgagata cttcttcttc
      901 gtctcctcgg agccgccgag gacgatcggc gcggtcgcca ggccgttgca catcagtgag
      961 gtgccgatgc cggagcagcc ccagccgatc tcctcctcga tcacgcaacc ggtcaggaag
     1021 tcgagtcccg gaccgccgta ctcggccggg atgtgggtgt tcatcaggcc gacgtcccac
     1081 gccttctcga tgatctcccc gggccaggtg ccctccttgt ccagctccca ggcgaccttg
     1141 cgcatctcct tctcggcgaa gtcgtgcgcc atcgactgga tgtccttctg ctcatcggta
     1201 agcgtgaaat cgaccactgt cgctccttgg gatctcggaa gttcgcgggc ggcggggaca
     1261 ggcggaacgg agcgctggcg cccgtcccga ttgattcgtc agtcaaccct tcctcgaagg
     1321 gtacagaacc ggtcgccgga aagcattggc tggcgcccct gggccctgtc accctgaacg
     1381 catggcagag accttcggcc tcgtcgtaat cgtgatcgcg cttgtggctg ccctggccgc
     1441 ctgtgcgagc tacgcaggga gcggtcgcgt ctaccgcgcg atcggccgca gcgggctgtc
     1501 gctcgacgag ccggacctgc ggcccacccc gcaaccgggc agcgccgcct ggctcgccga
     1561 ggcggaggcc gagctgcgcc agctgctgga cgcgaagtcg gcgcgcagcg aggcccgtgg
     1621 ggatggggcg atcgacgtcg acgccgagct cgaacggctc tcccgcccgc agcgcaacgc
     1681 cgatcccgca ctgcgcgccg aggttcgcga gctcgtggtc gcgaccaacg agcgccgggc
     1741 gcggcgtggc cagccgccac tcgacgtcga ggcggaggtc gaccgtcggc tcgaggatct
     1801 cggcgcgtag ccgtcgccgc gtcgggtata cgtaggccat ggcagacggg cggcgcttcg
     1861 agatcgacga gcttctcgtc cgccccggga cgtacttcaa tccgcagacc gaggtgctcg
     1921 tcgtcgtcga cgactcgtcg tcgatggaca acgagatctt cgcgctcgag gagttcgagg
     1981 gcgcggactg gatcctgatc gccgacgacc tgccggtcga cgagccgcag cgcgacgagc
     2041 tgctcgagac cttccaggcg acctaccacg gcggcgacgg gcgccaaccg cgcgtgctcg
     2101 aagacccgga gagcgacgac gagggcgatc cgctcggcca ggaggaccac gagcggcacg
     2161 gcgcgagcat cgagcacgtc gacgacgagt agccggccgg gcccttaaag tcgcgccgcg
     2221 atgcgccctg tccactacct cgcggtcttc atcccggcgg cggtcgcgct cgagctggcc
     2281 cacgccgccc ccgtcctgat cttcggcgcc gccgcgctcg gggtgatccc gaccgccgcg
     2341 gtcatgggcg aggcgaccga gcagatcgcc gccaagaccg gccccggtat cggcggcttc
     2401 ctcaacgtca cgttcggcaa cgcgcccgag ctgatcatcg ccttcttcgc cctcaaggag
     2461 gggctgcagg aggtcgtcaa ggcgtcgatc gtcggctcga tcgtcggcaa catcctgctc
     2521 gtgctcggcg cggcgatgct cgtcggcggg ctcaagcgcg agaagcagac cttcaaccgc
     2581 accgccgcga ctgcccagtc ggcgatgctg ctgctggcgc tgaccgcact catcatgccg
     2641 gcgctcttcc agttgatcca cggcggcggg ctgccgggcg tcggcgacga gcgcgtcgac
     2701 ttcggctccg atctcgagac gctctcgcta ggcgtcgcgc tcgtgctgat gggcacctac
     2761 gcgctgggcc tcctcttttc gctgaagacc caccggcggc tgttcaaccc gttcgacgag
     2821 ggcgacgacc accacgccgg ctggtcggtc cgcaaggcgc tgatcgccct cggcgtctcg
     2881 gcggtgctgg tcggcctgat gtcggagatc ctcgtcggct cgatcggcga ggcatcggac
     2941 gagatcggcc tgtcggagtt cttcgtcggc gtcttcgtgg tcgcgatcgt cggcaacgcc
     3001 gccgagcact gggtcgcggt gctggtcgcg gccaaggaca agatggacct cgcggtcggc
     3061 atcgccgtcg gctcgagcgc tcagatcgcg ctgttcgtcg ccccggtgct ggtgctgctc
     3121 tcgttcgtcg tcgggccgga gccgatggcg ctcgtcttca acggctacga gatcggcggc
     3181 ctggtcttcg cggtgctgat cgccaacttc ctgacccagg acggcgagtc gaactggttc
     3241 gagggcgtcc agctactggc gttgtacgcg gtcctcggca tcgtcttctt cctcgcctag
     3301 tcgaggacgc gcctggcggc gtcatcggcg ccgcgcgggg cgggccccgc cacggcgcct
     3361 gcttccttgc cagccacgcc ctcgactaag gcgaacgcgt ctgctcggtg cggtgagccc
     3421 gctcggcctg cttgtcctcg gagtcggggt cgcggcggtc tgcgacgatc gaggcgacga
     3481 ttccgatcgc gaacaggacg gcgatgatcg cgaggctggc cagcgggccg acgtggacga
     3541 agtcctcgac gagcagcttg acgccgacga cgccgagcac gactgcgatc gtctggtcga
     3601 ggtagcggaa gcggcggatc aggccctcga cgaggacgaa cagggcacgc agcccgagca
     3661 gcgcgaagac gttgcccatc cagatcagga agggatcgcg cgtgatcgcg aaggcagctg
     3721 ggatcgagtc gatcgcgaag gcgatgtcgg cgaagacgac ggcagcgagg cagaggaaga
     3781 gcggcgtggc gtagcggcgg ccgtccttgc gcacgaacca gtgcttgccg tcgaaggtcg
     3841 agctgacggg atagaagcgc cggaccaggc gaaccagcag gttgcggtcg gggtcgacgt
     3901 tgtcgtcgac gccgcggaag atccggtagg cgagcgccag cagcgccacg ccgagcaggt
     3961 agatcacgaa gtggaactgc tcgatcagcg cgatcccgat caggatgaac agaccgcgca
     4021 ggaccagcgc cgcgacgatt ccccagaaca gcagccggcc gcgctgctcg tacgggacgc
     4081 cgaagtacga gaacagcagt aggaagacga agaggttgtc aagcgacagc gagcgctcga
     4141 tcaggtagac ggtcgtgtag aggacggcat cctcgccgcc ctcgagcacc cagacgacca
     4201 gcgccgccgc caggctcagc acgagccagc cgaccgacca ccagacggcc tccttgaagt
     4261 tcggctcgcg gccgcgggcg aagaagtgca ggtcgagcca gaggaagacc agcagcgcga
     4321 cggccagcgc ggcccaggga gcgatctcct cgaggatcat cgggcgggca cgctacacgc
     4381 ggaccggcta cggaagcagc gtgcggcagc gcgcgatagc tgcggagtcg ccgggatcgg
     4441 cggcgtcgag gcagtcggcg tagcgctcgt cgccgacgat cgagtcgagg cttccgatct
     4501 ggctcatcag ctcggagagc ggccgtgcgc tgcgcggcgc ctcgatccgc tggtcgccgc
     4561 cgacgtcgcc gagctgggcg gccaggcgaa tcgaagcgcc ggtgactccg ccgacgtcag
     4621 cgcggtcctg ctcaggcacc tcgacgtcga cccgcagcga caccctgcgc acgatgcggt
     4681 cgtcctcgcc gacatagacg tcgaaggccg ggttctcgac cgacctggca agccgctcga
     4741 tctcccgcgg ccgcaggggc cgcgccgcgt ccgcggcctc gccgagcgcg ccggccgagc
     4801 gccgcacgag tccgttgatg tcacgcagcg ccgcctcgac gtcgagcgtg ccggacacgt
     4861 gctcggtcgt cacgccacca acctcctcct cgccgtcgag ccgggcgtcg acgatccact
     4921 cgcgcgtgtc gaggccgagg tcggaaagcg agccgttggc gtcgccgcct cgggcgagcc
     4981 ggcggttggc acttgcgacc tgggcggcag gctgctcgta gaaggccccg ccgaacttca
     5041 ggaagacgcg gtcgccggtc gagagcagtc ccgactggat cgcctgaccg gcgccctggg
     5101 cctcgagcgc gatgtcgagg tcgagctgcg gcagcgcgtc tcgaccacgg acatacgggc
     5161 cgctggcgac gaccctcagc ggatcctcga agccggcgag gccctcgagg tcgagctgga
     5221 ggtcgatctc gaggtccgcg ctgggcactg gctcgtcgaa agcgcggtcg agcagtgcgc
     5281 gggcgtcttc gtcgcctcct cgaccgccct cgtctccgcc gccggcgcat ccggcgagcg
     5341 agatcggagc tgccaccgcc acgacggcca gcaggacggc ggtccacggc tgtcgaagtg
     5401 ctgtccggat ggtcacaagc tgcaaaggca gaaggggatg gcggagccta gcgggggatt
     5461 cgctgcatca cgcaacttcc gagccgccct acggttggct cctgagatga ccctccgaga
     5521 cagcgtcctg tgcaccagcg cgaccgtctg catcgggttg gcgctcgccg tgcccgcgca
     5581 agccggtgag ctgatcctgg tcggccacgg gaagccggcc gagcgagtcc acgacgcgtt
     5641 cgtcccgacc cgcggcgcgg gcgacctcgg gcgcgagcca gcgcgccggg ctcgccgctc
     5701 cggcgcgctc gccgccggcg cgaagctggc gcgaaggccc gcgcgcgtga cggcgccccc
     5761 acgcgctcgc gccgcccgga cgtcccgtcg cgcgcgaagg gcgacgagcc gtgcgctggc
     5821 gctcgcccgc aggcgcggcg cgatctcgtc ggcgcgatat gtgaagtacc gccggatcgt
     5881 gatgcgcgcg cgggcggtcg agcgccgtct gagcggtgcc cgcgccgccg agctcgcgaa
     5941 tgcgatcgcg ctcgtggacg gcatcgcgct gcggcgcaag ctgaccgccg ggcgcctgcc
     6001 ggcgctgatg ctgatcctcc agcgcaacac ccagtactgg ccgcgccagc cgttccccag
     6061 cgaccgcgac cacgtgatgt tccgcggcag ccggctgatc ttcgagtact acgccggcga
     6121 ggggctacag ctgcagccgc tggtcaactt caagcaggcg aacctgatgc acggggcgtg
     6181 cgtcaaggac accggcgagc cctgcgaccg tgcggggctc cgacgactgc tcagggagct
     6241 ggtccgcacc tcgacacggc gcggcggctt caagacctgg gagtactact tcgagttcgg
     6301 cggcggacgg ccgccgtgga tcagcggcat ggcgcaggcg accgcgatcc aggccttcgc
     6361 gcgtgcctcc gagctgctga acgcgccggg ctacctgaag caggcgcgtg ccgccttcgg
     6421 cgcgttccgc aagccgccac cgatcggcgt gcggaccacc ggtccgttcg gtgggacgca
     6481 ctacctccaa tactcgttcg cgccacgcct ctacatcatc aacgcgttcc tgcaggcact
     6541 gatcgggctc tacgactacg ccgagatcac cggcgatcgg accgcgcggg cgctactgga
     6601 ggacgccgag cccgaggccc gcgaggagct ggtcgcgaac gacaccggcg actggtcgac
     6661 ctactcctac cgcggcgcgg agtcgacgcg cgagtaccac gagctggtgc gcgagttcgc
     6721 ggccagcatg tgcagccggc tgcggcgcaa ggtctactgc gacacggcgc gcaacttcct
     6781 cagctacacg accgaccccg ccgagctcga gctgctcggg ccagcagccg ccaccaaacg
     6841 cgtaccgacc aacatccgct tctgggtctc gaagctctcc gcggtccagg tcacggtgac
     6901 gcgcaacggt cgcgtggagc tcgacaagct cgacaccttc cgccgcggca acggcgcgtt
     6961 cgagtggacg ccacgcgccg tgggcaccta ccggatccgc gtcgcggcca aggagctgcg
     7021 gacgggacgc ggcctgcgca gcagcgtcac aggcgcggtc gaggcgcagc tgccctaacg
     7081 ccaaggcgtc ggcgacccgc caggcgcctt gggggtctcg ggggcgtgcg cccccggttc
     7141 ccatccgtac gatgccgtag gtggcgccga gaaccattct ctacaccggc aagggcggtg
     7201 tcggcaagac gagcgtcgcg gcggcgagcg cgcggcgctg cgcggcggca gggctgcgca
     7261 cggtggtgat gtcgaccgac ccggcgcaca gcctcgccga ctcgctcgag accgagctcg
     7321 gcggcgagcc gaccgaggtc tcccccggcc tgttcggcca ggaggtgcag gctcaggacg
     7381 agctggagcg tcactgggac gctgtctcgg actggctcgg cgagctgctg gccgaccgcg
     7441 gcgtcgacga gatctcggct caggagctga cggttccacc cgggatggac gagctgttct
     7501 cgctgctgca gatcaagcgc caccacgagg ccggcgagtt cgacgtggtg atcgtcgact
     7561 gcgcgccgac cggggagacg ctgcgactgc tgtcgtttcc cgacatctgc acctggtggc
     7621 tcgagaaggt gttcccattc aggagccgcc tgctggccgc cgcgcggccg ctcgcgcgca
     7681 cggtgctcga cgtgccgatg ccgggcgagg ctgtgtttga ggaggtcgag cggctggcga
     7741 ccaacctggt cgcgatgaac gcaatcctgc gcgaccgatc gcgcacgtca gtgcgactgg
     7801 tgatcaaccc ggaccggatg gttgtcaagg aggcgatgcg gaccttcacc tacctcaacc
     7861 tctacggcta tctgaccgac gcggtcgtgg tcaaccgcgt actgccggcg gaggtcgccg
     7921 acggctactt cgcgggctgg cgcgcatccc agcaggagca catggagctc gtgcgctccg
     7981 gctttgcgcc cgtccccgtg ctgaccgcgc cgcacctgcc acgcgaggtg atcggcgcgg
     8041 acacactcga cttgctcgcg gacgagctct tcggcgactg cgacgtcgcc gagctgctgc
     8101 acgaggacct ctcgcgcgag ctgaccagcg acggcaacgg aaaggcgacg ctgcggctgc
     8161 cgctgccgtt cgccgaccgc ggcgacatcg acctcaagaa ggtcggcctc gaggtgatcg
     8221 tgcgggtcgg gacgcagaag cggacgatca tgctgccgcc cgccctggca ggctatgaga
     8281 ccggcggcgc gcggttccgg gacggcgcgc tcggtcacca cgatcggggc gacgccttcg
     8341 tggatcgcct ccttggtgat gacgatctcc cgcacgtcgt cccggtgcgg caggtcgaac
     8401 atgatgtccg tcatcatggt ctcgaggatg gagcgcaggc cgcgcgcgcc ggtgccgcgc
     8461 ttgagggcct tctgcgccac cgcgcggagc gactcgggat cgaaggtgag gcccacgtcc
     8521 tccagctcga acagcttctt gtactgcttg gtgagcgcgt tcttgggctc gaccaggatc
     8581 cgcatcagcg cgtcctcatc gagcgcctcg agcgagacca cgacgggaag acggcccacc
     8641 agctcgggga tgagcccgaa gcggagcagg tcgtcgggct ccacctcggc gtaggggttc
     8701 ttcttgctca gctcggggga gcgcttgcgc tcgtcgccgg cgaagccgat ctgctgccgg
     8761 ccggtactgg cctcgatgat cttctccaga ccgtcgaagg cgccgccgca gatgaacagg
     8821 atgtccttgg tgttgatctg gatgtattcc tgctgcgggt gcttccggcc gccctggggc
     8881 gggaccgacg cgatggtacc ctcgaggatc ttgagcaggg cctgctgcac gccctcgccc
     8941 gagacgtcgc gggtgatgct cgggttctcc gacttgcggg cgatcttgtc gatctcgtcg
     9001 atgtagacga tgccccgctc gcactcggcg acgttgaact cgccggcctg gagcagccgc
     9061 accaggatgt tctccacgtc ctcgccgacg tagcctgcct cggtgagagt ggtcgcgtcg
     9121 gcgatggtga aggggacgtc gaggatgcgg gcgag
//
LOCUS       DATABV010000063         3524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1785892, whole genome shotgun sequence.
ACCESSION   DATABV010000063 DATABV010000000
VERSION     DATABV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785892"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..543
                     /locus_tag="VHF88_02540"
     CDS             76..543
                     /locus_tag="VHF88_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX2160675.1"
                     /translation="MSERKTWILTGSPENFAATREHGFKLIGMKERRRRQAEQFEPGD
                     EIVFYLTVVQAFAGIVRVTSELFEDRTKVWPGKPGNPDPYPWRFETEPLVVLDEPQWI
                     PAVELASELEHVGKWPIEHWHLAFQGQLRTVSQADAALLERRIRAAAGASTAA"
     gene            540..974
                     /locus_tag="VHF88_02545"
     CDS             540..974
                     /locus_tag="VHF88_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160676.1"
                     /translation="MTPEYGRPARAAAAVAETWRRLNVEQRVAAVAAVLVIVSTLGPF
                     SFVEAAEVLAAAAVLLLLRMRAERREFHLPLGDGTAIAGAGAWCAVLIATRMFDRPPG
                     QTALALLCALLLAGAGVRERARRAPDDLPDQRAETGRGSGPV"
     gene            complement(935..2020)
                     /locus_tag="VHF88_02550"
     CDS             complement(935..2020)
                     /locus_tag="VHF88_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HEX2160677.1"
                     /translation="MADVAQVQSGLEGVVAFATEIAEPDKEGGALRYRGVDIEDLVGA
                     VPFEKVWGLLVDGSFDPGLPPAIPHPLTVRTGDPRADLQAAVAMLAPEWGLKPVIDIT
                     DEQAREDLARVSVMALSFVAQSARGGGHPPVPQAEIDKAESIPARFLTRWRGEADPRH
                     VAAIDAYWISAAEHGMNASTFTARVITSTGADVSAALSGAVGALSGPLHGGAPSRVLS
                     MLDGVEQTGDAERWVKDALDRGERLMGFGHRVYRAEDPRARVLRRTAREIGAPRLEIA
                     EALEQAALAELQARKPDRVLATNVEFWSAVVLDFAEVPPELFTPLFSCARVAGWAAHI
                     LEQKREARLIRPTAIYTGPEPRPVSAL"
     gene            complement(2058..2288)
                     /locus_tag="VHF88_02555"
     CDS             complement(2058..2288)
                     /locus_tag="VHF88_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160678.1"
                     /translation="MSLLATLLAQAPFLDAGGGGDRAGRVSLEAFAAVLGAGFLIATV
                     GHVVRMRALVAFGIALIFGATVFVPALLALTD"
     gene            2333..3229
                     /locus_tag="VHF88_02560"
     CDS             2333..3229
                     /locus_tag="VHF88_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2160679.1"
                     /translation="MSAEFQSIEETRAGLADAGYLADESTALVSFLAGRLGKPVLVEG
                     PAGVGKTELAKALSRATGRKLVRLQCYEGLDEAKALYEWNYRKQLLRIQVEASDAGWQ
                     DVKDDIFGEEFLLERPLLTAIAAKEPVVLLIDEIDKTDQEFEAMLLELLSDFQISIPE
                     LGVIEARSRPIVVLTSNNSRELTEALKRRCLYLWLDYPDAERELEIVRLHAPDLPDSV
                     ARRLVEVVHMVRKLDLKKPPSIAESIDWARALLLLGAEDIDESMLQHTMSIIVKHRTD
                     LDMVAERVGVKLTDGLIGRQSA"
     gene            3201..>3524
                     /locus_tag="VHF88_02565"
     CDS             3201..>3524
                     /locus_tag="VHF88_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160680.1"
                     /translation="MGSSGASPPSGFAPRLLEFADELRGEGMHVGTSELLDAFAALEH
                     VPWTREEDFRAALAATLAKSQEDRRVFDILFDRWFFRAAEREAVERGIGEHRFDEDGG
                     YQGDQN"
BASE COUNT          535 a         1247 c         1263 g          479 t
ORIGIN      
        1 gcgacacctg gccggtaaag aaccgggggc tcacgccccg gacccccgcg acacctggcc
       61 ggtagcgtgt cgtcggtgtc cgagcgcaag acctggatcc tcaccggctc gcccgaaaac
      121 ttcgccgcga cgcgcgagca cgggttcaag ctgatcggga tgaaggagcg acggcggcgc
      181 caggccgagc agttcgagcc cggcgacgag atcgtcttct acctcacggt cgtccaggcc
      241 ttcgcgggca tcgtgcgcgt gacgtctgag ctgttcgagg accgcaccaa ggtctggccc
      301 ggcaagcccg gcaaccccga cccctatccg tggcgcttcg agaccgagcc gctggtcgtg
      361 ctcgacgagc cgcagtggat accggcggtc gagctcgcga gcgagctcga gcacgtcggc
      421 aagtggccga tcgagcactg gcacttggcg ttccaaggcc agctgcggac cgtgtcgcaa
      481 gcagatgccg cgctgctcga gcggcgcatc cgggcggcag cgggcgcctc gacggcggcg
      541 tgaccccgga gtacggtcgc cccgcgcgcg cggcggcagc ggtcgccgag acttggcgcc
      601 ggctgaacgt cgagcagcgc gtcgcagccg tcgccgcggt cctggtgatc gtcagcacgc
      661 tggggccgtt ctcgttcgtc gaggccgccg aggtccttgc tgcagccgcc gtgctcctgc
      721 tgctgaggat gcgcgccgag cggcgcgagt tccacctgcc gctgggcgac gggacggcga
      781 tcgccggcgc cggggcgtgg tgcgccgtcc tgatcgcaac gcggatgttc gaccgcccgc
      841 ccggccagac cgctctcgcg ctgctctgcg cgctgctgct tgcgggcgcc ggcgttcgcg
      901 agcgcgccag gcgagcgccc gacgacttac cggatcagag ggcggagacc ggccgcggct
      961 ccggccccgt gtagatcgcg gtcgggcgga tcagccgggc ctcgcgcttc tgctcgagga
     1021 tgtgcgccgc ccagccggcg acgcgggcgc aggagaacag cggcgtgaac agctcaggcg
     1081 ggacctcagc gaagtcgagc acgaccgccg accagaactc gacgttggtc gccagcacgc
     1141 ggtcgggctt gcgcgcctgc agctcggcga gcgccgcctg ctcgagcgcc tcggcgatct
     1201 cgagccgcgg cgcgccgatc tcgcgtgcgg tgcggcgcag gacgcgtgcg cgcggatcct
     1261 cggcacggta gacgcggtgg ccgaagccca tcagccgctc gccgcggtcg agtgcatcct
     1321 tgacccagcg ctccgcgtcg ccggtctgct cgacgccgtc gagcatcgac agcacgcgcg
     1381 agggggcgcc gccgtgcagc ggccctgaga gcgcgccgac cgcgcccgac agagcggccg
     1441 agacgtcggc accggtcgag gtgatcacgc gtgccgtgaa ggtcgaagcg ttcatgccgt
     1501 gctcggccgc tgagatccag taggcgtcga tcgccgcgac gtggcgcgga tccgcctcgc
     1561 cgcgccagcg ggtcaggaag cgggcgggga tcgactcggc cttgtcgatc tcggcctgcg
     1621 ggaccggcgg gtggccgccg ccacgtgcgg actgggcgac gaacgacagc gccatcaccg
     1681 acacgcgcgc caagtcctca cgagcctgct cgtcggtgat gtcgatcacc ggcttcagac
     1741 cccactccgg cgccagcatc gcgaccgcgg cctggaggtc ggcgcgcgga tcgccagtgc
     1801 ggacggtcag cgggtgcggg atcgcgggcg gcaggccggg gtcgaaggag ccgtcgacga
     1861 gcaggcccca gaccttctcg aacggcaccg cgccaaccag gtcctcgatg tcgacgccgc
     1921 ggtagcggag ggcgcccccc tccttgtccg gctcggcgat ctccgtcgcg aaggcgacta
     1981 cgccctcgag acccgactgc acctgcgcta cgtccgccat tcagcctcct tgctggtctc
     2041 tcggcgagcg cacgaggtta gtcggtcagc gccagcagcg cgggaacgaa gaccgtcgcc
     2101 ccgaagatca gcgcgatgcc gaacgcgacg agcgcccgca tcctcacgac atgaccgacc
     2161 gtggcgatca gaaagcccgc cccgagcacg gctgcaaagg cctccagcga cacacggccc
     2221 gcccgatcgc caccgcctcc ggcatcgagg aacggggcct gggcgagcag cgtggcgagg
     2281 agggacaccg cccgaaggta tcccaagcgg ggccggcggt agccttctcg tggtgagcgc
     2341 ggagttccag tcgatcgagg agacccgcgc gggcctggcc gacgcgggct acctggccga
     2401 cgagtcgacg gcgctcgtct cgttcctcgc cggacggctc ggcaagcctg tgctggtcga
     2461 ggggccggcg ggcgtcggca agaccgagct tgcgaaggcg ctgtcgcgcg ccaccggccg
     2521 caagctcgtg cgcctgcagt gctacgaggg gctcgacgag gccaaggcgc tctacgagtg
     2581 gaactaccgc aagcagctgc tgcggatcca ggtcgaggcc tccgacgccg gctggcagga
     2641 cgtcaaggac gacatcttcg gcgaggagtt cctgctcgag cgaccgctgc tcaccgcgat
     2701 cgcggcaaag gaacccgtcg tgctcctgat cgacgagata gacaagaccg accaggagtt
     2761 cgaggcgatg ctgctcgaac tcctgtccga cttccagatc tcgatcccag agctcggagt
     2821 gatcgaggcg cgcagccggc cgatagttgt cctgacctcg aacaattcgc gcgagctgac
     2881 cgaggcgctc aagcgccgct gtctctacct gtggctcgac taccccgacg ccgagcgcga
     2941 gctcgagatc gtgcggctgc acgcgcccga cctgcctgac tccgtcgcgc gccggctcgt
     3001 cgaggtggtc cacatggtcc gcaagctcga cctgaaaaag ccgccgtcca tcgcggagtc
     3061 gatcgactgg gcgcgcgcgc tgctgctcct gggcgcggag gacatcgacg agtcgatgct
     3121 ccagcacacg atgtcgatca tcgtgaagca ccgcactgac ctcgacatgg tcgccgagcg
     3181 cgtcggagtg aagctgaccg atgggctcat cgggcgccag tccgcctagc ggcttcgccc
     3241 cgcggctgct cgagttcgcc gacgagctgc gcggcgaggg catgcacgtg ggcacgtcgg
     3301 agctgctgga cgccttcgcc gcgctcgagc acgtgccgtg gacgcgcgag gaggacttcc
     3361 gtgcggcgct cgcggccacg ctcgcgaagt cgcaggagga ccggcgcgtc ttcgacatcc
     3421 tgttcgaccg ctggttcttc cgcgccgcgg agcgcgaggc cgtcgagcgc ggcatcggtg
     3481 agcaccgctt cgacgaggac ggcggctacc agggcgacca gaac
//
LOCUS       DATABV010000064        17704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1303817, whole genome shotgun sequence.
ACCESSION   DATABV010000064 DATABV010000000
VERSION     DATABV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17704
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303817"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VHF88_02570"
     CDS             <1..678
                     /locus_tag="VHF88_02570"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2160681.1"
                     /translation="SLKSAVGLGTLLADGVGDTIRISLSTFHAEEEVKVGWEILKALK
                     LRERGPVLIACPTCGRLQFDMDTVVAEIEQRLESYVEPIEVAVLGCAVNGLGEAAHAD
                     FGITGAKNEGLIFAHGKPLRKVAQERLVEELFTEIDRSLKVGRTVFDDREAAAGAQWL
                     ERIEEENAGELTPERLAALEAKAASAEAMSNATELPMAPAAKAERKAVSLGEDASPTA
                     GRRFTRA"
     gene            complement(679..2670)
                     /locus_tag="VHF88_02575"
     CDS             complement(679..2670)
                     /locus_tag="VHF88_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2160682.1"
                     /translation="MHDPSRLDNHHWIPIVVGLAWVLFALHSLDLFGDAADAFFDSWL
                     YMGLLAAAAAGCLARALSAPAERLAWALMGAAIAVWSLGELAWSSQLATLAGAGTPSI
                     SDSLYLAFYPLAYATLVVLIRRQAGLASRDLWLDGVIGASCATAFAIAVAFPAFDVNG
                     DDALELATTLAYPVLDIVLLSFVILVFGLSGWRPGRRWTLIGAGLTAMALSDGIYLVQ
                     VAHGSYAEGGPIDAGWPAAALLVALAAWQPPGTPGAPRETDRRLVLVPVFCGLVALAL
                     VTLAVFEALPQEAAPPALLTLLLVTGRMGMAFIEGQHTMDASRREALTDGLTGLGNRR
                     ALLADLERAVMRATADDPRVCVLLDLNGFKAYNDAFGHPAGDELLHRLGQRLQEVVDG
                     HGCAYRLGGDEFCVLGRRHVGGGRPLADIARAALTETGEAFAVTSSAGIVELPIDAQT
                     PADVLQVADRRMYADKGRRSSSAGHQSRAVLLRILREREPEMHDHLVGVAELALAVAR
                     ELGMAGEELDELARAAELHDIGKIAVPDEILRKPGPLDRDEWAFVRSHPVIGERILGA
                     APALRPVARIVRSSHERWDGSGYPDGLAGEQIPLGARIVAVCDAYEAMVSDRPYRPAL
                     TPEQAVAELREHAGSQFDPTVVRVFTAALSTRSPEPLAS"
     gene            2794..4491
                     /locus_tag="VHF88_02580"
     CDS             2794..4491
                     /locus_tag="VHF88_02580"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2160683.1"
                     /translation="MTRQSAYLLPTEKEAPADAEAVSHKLMVRAGLIRQLGAGMWTYL
                     PAGWRAHRRVEGIIRREQDAIGAQEMLMPVLQPADPWRRTGRYEIDELFKLEDRRGAE
                     MVLAMTHEEALTFHVAREVRSYRDLPLMLYQFQVKERDEPRPRAGVLRTREFVMKDAY
                     SFDRDAAGLDRSYELQVGAYDRIFERSGLEWYRVEADVGMMGGVGAHEYMAPCAAGEN
                     EVALSDAGYAANVEVASAQAQTVDGLPAGLDGPEPVDTPGASSIEDVARLLGVPPGAL
                     VKALPVIRDDGQPLLVLVRGDHRLNEIKLQNHLGAPARQATEDEIRSAFGAPPGFVGP
                     VDARVPVLADEALRGLRGLVAGANRPDRHLRGVEPGRDFDADWADVRDVVAGDRCPRG
                     GTIRIEPAIEVGNIFKLGTRYSAPLGARFLDGSGKEQDVWMGSYGIGPARIVAAAVEQ
                     YADEQGIAWPRALAPFDVHLVTLGKEGEEARAVADGIYEQLLATGLEVLYDDRNASAG
                     EKFADAELLGAPLRLTVGRKSVEAREVEAQVRRGQERRTLPLDGAADAAAELWRALP"
     gene            4473..5111
                     /locus_tag="VHF88_02585"
     CDS             4473..5111
                     /locus_tag="VHF88_02585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2160684.1"
                     /translation="MARPTLRPSRRLLGIDRSGGPPPETQKGQPLRPWTVPNAISYVR
                     LALLPVFLAVALGSDDGREPAAAALYFGIAWGDQLDGLAARLTGQYSRLGALLDPLTD
                     RALVIAGVVVCWHFELLPRWALAVLAARELLMLVLTQAGLRAGMDLRINMVGRWAVWP
                     VMFAIFLALIVESWVAPVSLYLGLALTLWATAIYLADGYRFLQRRDRPSSST"
     gene            5164..5424
                     /locus_tag="VHF88_02590"
     CDS             5164..5424
                     /locus_tag="VHF88_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160685.1"
                     /translation="MDDDTFPDLGALSDQELKELIDDYTAEEQEVSYRRRILHGKIDI
                     LRAELVNRLRKKHEAGESALSGADVQQLTDILAGKAIPDGDA"
     gene            5438..5905
                     /locus_tag="VHF88_02595"
     CDS             5438..5905
                     /locus_tag="VHF88_02595"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2160686.1"
                     /translation="MHCPECGFINPDGANYCQKCGAFLPETEEKGSGSTTAAYQLDES
                     GELRPVDIDEVAAEGATLAIRSGGGRAGEVFEVKGDRTQIGRSPDAEVFLDDVTVSRN
                     HALLVRRRDGLYVDDLGSLNGTYVNRRRIESHKLANGDELQVGKYKLTYLERQ"
     gene            5902..6639
                     /locus_tag="VHF88_02600"
     CDS             5902..6639
                     /locus_tag="VHF88_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2160687.1"
                     /translation="MTQAEIDTTDMPPIQGKALTIGAVCKQLEREFPDISISKIRYLE
                     DQKLLTPRRTAGGYRLFTAADVMRLRTILRLQRDEFLPLRVIRQELASGRAESDDVSK
                     PSGPDPKSLRRATVAGAAESLYRLDEVLDETGAEPTLVKELEEYGVIRGERRDGEMLY
                     DETDREIVRAVTELKRYGVGGRNLRVFRTSADREAALLEQILAPALRSRNPERRKEAV
                     ETLENLAAIASHLKHLLLIRDLRKIAK"
     gene            6658..7089
                     /locus_tag="VHF88_02605"
     CDS             6658..7089
                     /locus_tag="VHF88_02605"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2160688.1"
                     /translation="MSRSRVVAAIPEHVWDVVADPGRLPEWWPNVQRVEEADGKAWTT
                     VLASPKGGRTLRADFTLLASEHPRRRSWRHEVAASPFERVLTDSVTDIRLERAGDGQT
                     EVRISEQLGLRGLSRLGGGQVKRATRRKLDGALDGLERLFG"
     gene            7086..8708
                     /locus_tag="VHF88_02610"
     CDS             7086..8708
                     /locus_tag="VHF88_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2160689.1"
                     /translation="MMRWWGWGRDEDAIELPPAAAELLRGELGLSGDERGARVALDEV
                     QLAPARLGERARRALEDIVGASAVMDGREARVAHAAGRGYPDLVRLRAGDARGAPDAV
                     ALPSESAQLAPLLAACAEHRVAVIPFGGGTSVVGGVEALPGEHHAAVTLDLSRLDAVI
                     DVDARSLTATVEPGIFGPALERQLGGSGLTLGHFPQSFEYSTVGGWVATRSAGQASTG
                     YGRIDELVEAVRCTTPAGELATLDVPASAAGPSLRELVVGSEGVLGVISAATLRVRPA
                     PAEQRYEAWSLPSFEAGAEAFRDLAQIGATPDVARLSDEDETRLTMALGSSGGLAEKA
                     GKLYLRAFGSADGCIAFTGFDGRADDIARRRGRTAEILRAHGAVALGTRPGRSWLRGR
                     FAAPYLRDELMDRGVMVETLETATTWSNLQRLYAAVGGALRTSLAEGGTPPAVMCHIS
                     HLYPSGASLYFTFVAGQRRGEEIAQWRAAKTAACDAIAATGGTITHHHAVGTDHRAWM
                     ASEVGELGVDVLRAVKARVDPAGIMNPGKLLP"
     gene            complement(8736..9725)
                     /locus_tag="VHF88_02615"
     CDS             complement(8736..9725)
                     /locus_tag="VHF88_02615"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX2160690.1"
                     /translation="MASYDQLSAQQRAIVDLILKRGQSYDQLADTLGMPEPRVRELAG
                     EALTSLAPVSARAVDDDWRSEIADYLLGQQDPKQATATRGHLRRSEPARAWSRSVLDS
                     LEHLYPNGELPTIPSSDGGPAKSKPPGKAGEPKAATPRRELSPEAAAVVRRRRLAAAA
                     ATAVALLLIVLVWPVGLLTGDDSDDGGDSTSGMQTELIGQGVLRAVPDSGANRNRHAG
                     LAVITRRGEQFELAVEARLPPTEQNQAYEVWLYNSPEDAVSLGAQVTDRQGAFAGRAK
                     LPDDYRDYRFLEISRETVDRNADHSGESILRTRVSSIRPPQGDGGGAGQGAQP"
     gene            9752..10165
                     /locus_tag="VHF88_02620"
     CDS             9752..10165
                     /locus_tag="VHF88_02620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2160691.1"
                     /translation="MTDEAAAAAQTPGQTLNDVLGIEVGEFDEQSITGGFEVADRVRQ
                     PFGIVHGGAYAAAAETLASVATFYAVNANGEIAVGQNNDTSFLRPVSAGRVHAVGTCR
                     HRGRTSWIWDFEFTDDQDRVCAISRVTIAVRPAPS"
     gene            complement(10134..11921)
                     /locus_tag="VHF88_02625"
     CDS             complement(10134..11921)
                     /locus_tag="VHF88_02625"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX2160692.1"
                     /translation="MSPRPEPLRCALAQINSTVGDIDGNADRIAAATVQARERGAALV
                     VFPELALTGYPPEDLLLRPHFLDEAAAALEQLAAQTIDVVAVVGFPERADDVYNAAAV
                     LADGRVAGVYRKLRLPNYGVFDEQRYFQAGETPATVEVNGVTVGITVCEDIWEPGPPA
                     TAEALAGAQVVVNLSASPYHHGKGLQRERMLAQRARDSLVSVLFCNAVGGQDELVFDG
                     HSLAIDHSGAIVARAPQFEESITVCSLDVGPIAAGRLRDPRHREAARRARSGHSVPVV
                     RLGSLRASSRPTMPGSDVLPAPRAELLDPEDEVYAALVTGVRDYVDKNGFGGAVLGLS
                     GGIDSALAALVAVDALGAERVTTVVMPSPHSSDATQADARTIAVNLGAGLYELPIGAA
                     MEVYEELLAEVFAGHEPDLTEENLQARIRGNLLMALSNKFGWLVLTTGNKSELSVGYS
                     TLYGDMAGGFAVLKDVFKGWVYRLVRWRNAQAGTELIPSSVIERAPSAELRADQRDED
                     SLPPYELLDAILAGYVEQDLDPAGLVARGYPAEEVARVIALVDAAEHKRRQAPPGVRI
                     STRAFGRDRRLPITNRYGRSKKAPVAPRS"
     gene            complement(11942..12394)
                     /locus_tag="VHF88_02630"
     CDS             complement(11942..12394)
                     /locus_tag="VHF88_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2160693.1"
                     /translation="MRSKVIDSARDLGLDVNVQRLPDTRASVADAAAAVGCGAARIVS
                     CAVFVADGDPVVCVASADRVVDTEALADVLDVAEVRPASGSETRAATGFPASGVPPLG
                     HGLPVVLDAALLEHASVWAAGGDGHTLVELEPERLASCTAATVAAVTA"
     gene            complement(12509..13969)
                     /locus_tag="VHF88_02635"
     CDS             complement(12509..13969)
                     /locus_tag="VHF88_02635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX2160694.1"
                     /translation="MQGDRLSGLDASFLHLEDDSAHMHVASVIVFEGKAPEYVELLEH
                     VERRLHLVPRFRQRLAFVPLGQGRPRWVDDPHLNLRYHLRHSGLPAPGSEQQLRALAG
                     RVFAQPLDRDKPLWELFLVDGLEGDRFALLSKTHHALVDGISGVDIVTVLFDTQPEPV
                     APADPGTPWLPRPLPSQAQLLGEALVERATIPAETGRTLRAVFRAPRRIAREAVNTAA
                     GLGAMAWAGLSPAPTTPYNRPIGPHRRFTWVRANLADVKAIKNELGGTVNDVVLTAVA
                     GALGRHLRRRGVSTEGLELKAMVPVSVRSQEQVGELGNQVAAMMAPLPVWCQDPVARQ
                     QIVRQAMDHLKDGGQAVGAQALTEMSGFAPPTIMGQAARLMSRQRFFNLVITNVPGPQ
                     IPLYLLGRRALDPFPLVPLAKNQGVGIAIMSYDGRMDFGLVGDYDVMYDLDDLARDLH
                     ESLAELAEAAGRTLSSPPPDAPAEQLHASDAVARSK"
     gene            complement(13983..15065)
                     /gene="dinB"
                     /locus_tag="VHF88_02640"
     CDS             complement(13983..15065)
                     /gene="dinB"
                     /locus_tag="VHF88_02640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX2160695.1"
                     /translation="MSDDGDRRIAHLDMDAFYVNCELQRRPELRGKPVVVAGSGPRAV
                     VTTASYEARRFGVFSATPAARARRLCPQALFVPPDFDYYRSRSRAVMEVLHAHVERVE
                     QVGLDEAYLDFTGIERWRAAARRLKRAVTEATGLACSIGIGPNKLVAKVASDADKPNG
                     FLALTLDEARERFAGASPALIPGIGPKTVERLSARGIDRLDQLAATPDATLGEWFGGR
                     LGPQLARLARFEDERQLETERVRKSESRETTFDRDLSGLAELEPVLERLARQLSEELA
                     EHGRSGRTIGIKVRLDDFSTLTRARTVERPANDFATVHGVALELLRALDPARPVRLLG
                     VRVAAMEERRDPQAAAPPPGQLALTL"
     gene            15200..16849
                     /locus_tag="VHF88_02645"
     CDS             15200..16849
                     /locus_tag="VHF88_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX2160696.1"
                     /translation="MIAASAAEHIELVVFGLLVAVSALAVVARLVRIPYPILLVIGGV
                     AIGFVPEIPNIELDPDIVLLVFLPPLLYAAAFFSNLRELRANVRPIGLLAIGLVIVTT
                     LAVAAVAHWAIGLEWQVAFVLGAIVSPTDAVAPATILRRLGVPRRVVTIVEGENLTND
                     WTALVTYKFAVAAVVTGSFTLVDAGPDFILTGVGGAAIGVAGGLAIAAVRRRLDDPPT
                     EITISLLTPYAVYLPAEELGVSGVIAAVTVGVLLGWRASELTTHTTRLQSYAIWEILQ
                     FLLNAVLFLLIGLQLPTALDALDERNGGELLGYAVLVSAVAILVRIAWVYALSAVDRN
                     VRSEISGPDDGAAWKEVTLVAWSGMRGGVSLAAALAIPLQTDTGAGFPERDLVLFLTF
                     SVIIATLVLQGLAFPLLIRALDLDEDSSDADEELTARIETAYAALDRIEELTEEEWVP
                     RDTLARVRDLYDYRRRRFSSRLDDRDPEDGFDYEQRAELYRRVMNEVIDAQRAALRRL
                     RDDGEITDEVRRNVEHELDLEQSRLERSDSSTQPRSATISA"
     gene            complement(16819..17601)
                     /locus_tag="VHF88_02650"
     CDS             complement(16819..17601)
                     /locus_tag="VHF88_02650"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX2160697.1"
                     /translation="MAESFYVADGVGFAATELTRGPWDEGSQHAGPPCALLGREIDSA
                     GRLEHGQLARITYEILRPVPIARLTTVAEVVRPGRSVELVEGTLLHDGEAVIRARAWR
                     IRTAEVEIDRPPPEPARIPGPANAATQSFFPVPWDVGYHSAMEIRLVEGGFVDPGPAR
                     AWMRMRVPLIDGEEPLPRDRVLVAADSGNGVSAPLDYRRYIFINTDLTVNLRRLPEGE
                     WVCLDAVTYPEASGIGLSDSALHDERGMIGRATQALMVAERG"
BASE COUNT         2455 a         6335 c         6525 g         2389 t
ORIGIN      
        1 tcgctgaagt cggccgtcgg cctcggcacg ctgctcgccg acggggtggg ggacacgatc
       61 cggatctcgc tctcgacctt ccacgccgag gaggaggtca aggtcggctg ggagatcctc
      121 aaggcgctca agctgcgcga gcgcggcccg gtgctgatcg cctgccctac ctgcgggcgg
      181 ctgcagttcg acatggacac cgtggtcgcg gagatcgagc agcggctcga gagctacgtc
      241 gagccgatcg aggtcgcggt gctcggctgc gcggtgaacg gcctcggtga ggcggcccac
      301 gccgacttcg ggatcaccgg cgccaagaac gagggcttga tcttcgccca cggcaagccg
      361 ctgcgcaagg tggcgcagga gcgcctcgtc gaggagctct tcaccgagat cgaccggtcg
      421 ctgaaggtcg gccgcacggt cttcgacgac cgcgaggccg cagcgggggc gcagtggctc
      481 gagcggatcg aggaggagaa cgccggcgag ctgacgcccg agcggctggc ggcgctcgag
      541 gcgaaggcgg ccagcgccga ggcgatgtcg aacgccaccg agctgccgat ggcgcccgcg
      601 gcgaaggccg agcgcaaggc cgtcagcctc ggcgaggacg ccagccctac ggctggccgc
      661 cgcttcacgc gcgcctgatc agctcgcgag cggctcgggg gaccgcgtgc tcagcgccgc
      721 ggtgaagacg cgcacgacgg tcgggtcgaa ctgggagccg gcgtgctcgc gcagctccgc
      781 gacggcctgc tccggggtca gggcggggcg ataggggcgg tcggagacca tcgcctcgta
      841 ggcgtcgcag accgcgacga tgcgcgctcc gagcgggatc tgctcacccg ccaggccgtc
      901 cgggtagccg ctgccgtccc agcgctcgtg gctcgagcgg acgatccgcg cgaccggtcg
      961 caacgccggc gccgccccga ggatccgctc gccgatcacc gggtggctgc gcacgaacgc
     1021 ccactcgtcg cggtcgagcg ggccgggctt gcgcaggatc tcgtcgggga cggcgatctt
     1081 gccgatgtcg tgcagctcgg cggcgcgcgc gagctcgtcg agctcctcgc cggccatgcc
     1141 cagctcgcgc gcgacggcca acgccagctc ggcgacgccg acgaggtgat cgtgcatctc
     1201 cggctcgcgc tcgcgcagta tgcgcagcag cacggcacgc gactgatggc cggccgacga
     1261 gctgcgccgg cccttgtcgg cgtacatgcg acggtcggcg acctgcagga cgtccgccgg
     1321 cgtctgggcg tcgatcggca gctcgacgat gccggccgag ctggtcacgg cgaacgcctc
     1381 gccggtctcg gtcagcgctg cgcgcgcgat gtcggcaagc ggacggcccc cgccgacatg
     1441 gcggcggccg aggacgcaga actcgtcgcc gccgagccgg taggcgcagc cgtgcccgtc
     1501 gaccacctcc tggaggcgtt gaccgagcct gtgcagcagc tcgtcgccgg ccggatgccc
     1561 gaaggcgtcg ttgtaggcct tgaagccatt caggtcgagc agcacgcaga cgcgcggatc
     1621 gtcggctgtc gcgcgcatca ccgcccgctc caggtcggcg agcagggcgc gccggttgcc
     1681 gaggcccgtc aggccatccg tcagcgcctc gcgccggctc gcgtccatcg tgtgctgccc
     1741 ctcgatgaac gccatcccca ttcgcccggt caccagcagc agagtcagca gcgctggcgg
     1801 agcggcctcc tgcggcaggg cctcgaagac ggcgagcgtc actagcgcga gcgcgaccag
     1861 cccgcagaag accggcacga gcaccagacg gcggtcggtc tcgcgcggtg cgccgggcgt
     1921 tccgggcggt tgccacgcgg caagtgcgac cagcagcgcg gcggccggcc agccagcgtc
     1981 gatcggcccg ccctcggcgt agctgccgtg tgcgacctgg acgaggtaga tgccgtcgga
     2041 gagcgccatc gccgtcaggc cggcgccgat cagggtccag cgacgcccgg gtcgccaccc
     2101 ggagagcccg aagacgagga tcacgaagct cagcaggacg atgtcgagca ccggatacgc
     2161 gagcgtcgtg gcgagctcga gcgcgtcatc gccgttgacg tcgaaggccg ggaacgcgac
     2221 cgcgatcgcg aacgcagtcg cgcacgaggc gccgatcacg ccgtcgagcc agaggtcgcg
     2281 ggaggcgagc cccgcctggc ggcggatcaa gaccaccaac gtcgcgtagg cgagcggata
     2341 gaacgccagg tagagcgaat cggagatcga cggcgtgccg gctccggcga gcgtcgccag
     2401 ctgggagctc caggcgagct ctccgagcga ccagacggcg atcgccgcgc ccatcagcgc
     2461 ccacgcgagc cgctcggccg gcgcggagag ggcgcgggcg aggcagccgg ccgcggcggc
     2521 ggcgagcagt cccatgtaga gccagctgtc gaagaaggcg tccgccgcgt cgccgaagag
     2581 gtcgagcgag tgcagcgcga acaggaccca cgcgaggccg accacgatcg ggatccagtg
     2641 gtggttgtcg agacgtgacg ggtcgtgcat cgcggtccgc gggacaatgc ccagagaggc
     2701 cttccgtcta tcggcattcg gggggaggtg attgaacggc gggcggtcgt gcccgtcgcg
     2761 ccccgggccc ggtgccgact agactcgccg ccgatgacgc gccaaagcgc ctacctgctg
     2821 ccgaccgaga aggaggcgcc cgccgacgcg gaggcggtct cgcacaagct gatggtgcgc
     2881 gccgggctga tccgccagct gggcgccggc atgtggacct acctgccggc cggctggcgc
     2941 gcccaccgcc gcgtcgaggg gatcatccgc cgggagcagg acgcgatcgg cgcgcaggag
     3001 atgctgatgc cggtgctgca gccggccgat ccctggcggc ggaccgggcg ctacgagatc
     3061 gacgagctgt tcaagctcga ggaccgcaga ggcgcggaga tggtcctggc gatgactcac
     3121 gaggaggcgc tgaccttcca cgtcgcgcgc gaggtccgct cctaccgcga cctgccgctg
     3181 atgctctacc agttccaggt caaggagcgc gacgagccgc gtccgcgcgc cggcgtcctg
     3241 cgcacgcgcg agttcgtgat gaaggacgcc tactcgttcg accgtgacgc cgcgggcctc
     3301 gaccgctcct acgagctgca ggtcggcgcc tacgaccgga tcttcgagcg ctcggggctc
     3361 gagtggtacc gcgtcgaggc agacgtcgga atgatgggcg gcgtgggcgc gcacgagtac
     3421 atggcgccct gcgccgcggg cgagaacgag gtcgcgctgt cggatgccgg ctatgcggcg
     3481 aacgtcgagg tcgcgagcgc acaggctcag accgtcgacg ggctgcccgc cgggctcgac
     3541 ggccccgagc cggtcgacac gccgggcgcc tcgtcgatcg aggacgtcgc gcgcctgctc
     3601 ggcgtgcccc ccggagcgct ggtcaaggcg ctgccggtca tccgcgacga cgggcagccg
     3661 ctgctcgtcc tggtccgcgg cgaccaccgg ctcaacgaga tcaagctgca gaaccacctc
     3721 ggggcaccgg cgcgccaggc gaccgaggac gagatccgct cggccttcgg cgcaccgcca
     3781 gggttcgtcg ggccggtcga cgcccgtgtg ccggtgctcg ccgacgaggc gctgcgtggg
     3841 ctgcgcggac tggtcgccgg agcgaaccgg cccgatcggc acctgcgcgg ggtcgagccg
     3901 ggacgcgact tcgacgcgga ttgggctgac gtccgcgacg tcgtcgccgg cgaccgctgc
     3961 ccgcgcggcg gcacgatccg catcgagccg gcgatcgagg tcggcaacat cttcaagctt
     4021 ggcacccgct actcggcgcc gctcggcgcg cggttcctcg acgggagcgg caaggagcag
     4081 gatgtctgga tgggctccta cggcatcggt cctgcgcgaa tcgtcgcggc ggcggtcgag
     4141 cagtacgccg acgagcaggg gatcgcgtgg ccgcgcgcgc tggcgccgtt cgacgtccac
     4201 ctcgtgacgc tcggcaagga gggcgaggag gcgcgcgcag tcgccgacgg catctacgag
     4261 cagctgctcg ccaccggact cgaggtgctc tacgacgacc gcaacgcgag cgcgggggag
     4321 aagttcgccg acgccgagct gctcggcgca ccgctgcgcc tgaccgtcgg tcgcaagagc
     4381 gtcgaggctc gtgaggtcga ggcgcaggtg cgccgcggcc aggagcggcg cacgctgccg
     4441 ctcgacgggg cggccgacgc ggcggcggag ctttggcgcg ccctaccctg agaccgtcgc
     4501 ggcgcctgct cggcatcgac cgctccggcg ggccgccgcc cgagacccag aagggccagc
     4561 cgctgcgccc ctggaccgtt ccgaacgcga tctcctacgt ccggctggcg ctgctgccgg
     4621 tgttcctggc tgtcgcgctc ggctccgacg acggccgcga gccggctgcg gcggcgctgt
     4681 acttcgggat cgcctggggc gatcaactcg acggcctcgc cgcccgcctc accggccagt
     4741 acagccgcct cggcgcgctg ctcgacccgc tgaccgaccg cgcgctggtg atcgccggcg
     4801 tggtcgtctg ctggcacttc gagctgctgc cgcgctgggc gctggccgtg ctcgccgccc
     4861 gcgagctgct gatgctcgtc ctgacccagg ccggcctgcg cgccgggatg gacctgagga
     4921 tcaacatggt cggccgctgg gcggtctggc cggtgatgtt cgcgatcttt ctggcgctga
     4981 tcgtcgagag ctgggtcgcg cccgtgtcgc tgtacctcgg tctcgcgctg acgctctggg
     5041 ccacggcgat ctacctcgcc gacggttacc gcttcctgca acggcgcgac cgaccctcaa
     5101 gctccacttg acggctggct ctatactcgc ccttcggccc ccgaaacgaa ttggcgagag
     5161 cagatggacg acgacacctt cccggatctc ggcgcgctgt ccgaccagga gctcaaggag
     5221 ctgatcgacg actacaccgc cgaggagcag gaggtctcgt accgccgccg catcctgcac
     5281 ggcaagatcg acatcctgcg ggccgagctc gtcaaccggc tgcgcaagaa gcacgaggcc
     5341 ggcgagtccg cgctcagcgg cgccgacgtc cagcagctga ccgacatcct ggcgggcaag
     5401 gccatccccg acggcgacgc ctagccggga ggcggcgatg cactgccccg agtgcggatt
     5461 catcaacccc gacggggcga actactgcca gaagtgcggc gccttcctgc ctgagaccga
     5521 ggagaagggg agcggcagca ccaccgccgc ctatcagctc gacgagtccg gcgagctgcg
     5581 tccggtcgac atcgacgagg tcgcggccga gggcgcgacg cttgcgattc gctccggcgg
     5641 cggacgcgcc ggcgaggtct tcgaggtcaa gggcgaccgc acccagattg ggcgcagccc
     5701 cgacgccgag gtcttcctcg acgacgtgac ggtgtcgcgc aatcacgcgc tgctcgtgcg
     5761 gcgccgcgac ggcctctacg tcgacgatct gggatcgctc aacgggacct acgtgaaccg
     5821 ccggcggatc gagtcgcaca agctcgccaa cggcgacgag ctgcaggtcg gcaagtacaa
     5881 gctcacctac ctggagcgtc aatgacccaa gccgagatcg acacgaccga catgccgccg
     5941 atccagggca aggccctgac gatcggggca gtctgcaagc agctcgagcg cgagttcccc
     6001 gacatctcga tctcgaagat ccgctacctc gaggaccaga agctgctcac gccgcgccgc
     6061 accgccggcg gctaccgcct cttcacggcc gccgacgtga tgcgcctgcg gacgatcctg
     6121 cgcctccagc gcgacgagtt tctgccgctg cgcgtgatcc gccaggagct cgcgtcgggc
     6181 cgcgccgaga gcgacgacgt gtcgaagccg agcggtccgg atccgaagtc gctgcggcgc
     6241 gcgacggtcg ccggcgccgc cgagtcgctc taccggctcg acgaggtgct cgacgagacc
     6301 ggcgccgagc cgacgctcgt caaggagctc gaggagtacg gcgtgatccg cggcgagcgg
     6361 cgcgacggcg agatgctcta cgacgagacc gatcgcgaga tcgtgcgcgc cgtcaccgag
     6421 ctcaagcgct acggcgtcgg cgggcgcaat ctgcgcgtct tccgcacgtc cgcggaccgc
     6481 gaggcggcgc tgctcgagca gatcctcgcc ccggcgctgc gctcgcgcaa ccccgagcgg
     6541 cgcaaggagg ccgtcgagac gctcgagaac ctggccgcga tcgcctccca cctcaagcac
     6601 ctgctgctga tccgcgatct gcgcaagatc gctaagtaac cccgagcgat gccgactgtg
     6661 agccggagcc gcgtcgtcgc ggcgatcccc gagcacgtct gggacgtcgt cgcggacccg
     6721 gggcggctgc ccgagtggtg gccgaacgtc cagcgcgttg aggaggccga cggcaaggcc
     6781 tggacgaccg tgctcgcctc gccgaagggc gggcgcacgc tgcgcgccga cttcacgctg
     6841 ctcgcctcgg agcacccgcg ccgccgcagc tggcgccacg aggtcgcggc ctcgccgttc
     6901 gagcgcgtcc tgaccgactc ggtcaccgac atccggctcg agcgcgccgg cgacggtcag
     6961 accgaggtca ggatcagcga gcagctcggg ctgcgcgggc tgtcgcgcct cggcggcggc
     7021 caggtcaagc gcgcgacgcg gcgcaagctg gacggtgcgc tcgatgggct ggagcggctc
     7081 ttcggatgat gcgctggtgg gggtgggggc gcgacgagga cgcgatcgag ctgccaccgg
     7141 cggccgcgga gctgctgcgc ggcgagctcg gcctgagtgg cgacgagcgt ggggcgcgcg
     7201 tcgcgctcga cgaggtccag ctggcaccgg cccggctcgg cgagcgcgcc cgtcgggcgc
     7261 tggaggacat cgtcggcgcg agcgccgtca tggacggccg tgaggcgcgc gtcgcgcacg
     7321 ccgccggacg cggctacccc gatctggtcc ggctgcgtgc cggcgacgcc cgcggggcgc
     7381 ccgacgcggt ggccctgccg tcggagtcgg ctcagctcgc cccgctgctc gcggcatgcg
     7441 ccgagcaccg ggtcgccgtg atcccgttcg gcggtggcac gagcgtcgtc ggcggcgtgg
     7501 aggcgctgcc cggcgagcac cacgccgcgg tcacgctcga cctgtcgcgc ctcgacgcgg
     7561 tcatcgacgt ggacgcgcgc tcgctgaccg cgaccgtcga gccgggcatc ttcgggccgg
     7621 cgctcgagcg ccagctcggc ggctccggct tgacgctcgg ccacttcccg cagtcgttcg
     7681 agtactcgac cgtcggcggc tgggttgcca cccgctcggc cggccaggcc tcgaccggct
     7741 acggccggat cgacgagctc gtggaagctg tccgctgcac cacgccggcg ggcgaactcg
     7801 cgaccctcga cgtgcccgcg agcgccgccg ggccgtcgct gcgcgagctc gtcgtcggct
     7861 ccgagggcgt cctcggcgtg atctccgcag cgacgttgcg cgtgcggccg gcgcccgccg
     7921 agcagcgcta cgaggcatgg tcgctgccct cgttcgaggc aggtgcggag gcgttccgag
     7981 atctcgccca gatcggtgcg acgcccgacg tggctcgcct ctccgacgag gacgagaccc
     8041 gcctgacgat ggcgctcggc tcgagtggag ggctcgccga gaaggccggg aagctctacc
     8101 tgcgcgcgtt cggcagcgcg gacggctgca tcgccttcac cggcttcgac ggccgcgccg
     8161 acgacatcgc gcgtcgccgc gggcggaccg ccgagatcct gcgcgcgcac ggcgccgtcg
     8221 cgctcggcac caggccgggc cgctcctggc tgcgcgggcg cttcgcggcg ccctacctgc
     8281 gcgacgagct gatggaccgc ggcgtgatgg tcgagacgct cgagaccgcc acgacctggt
     8341 cgaacctgca gcgcctctac gcggcggtcg gcggggcgct gcgcacatcg ctcgcggagg
     8401 gcggcacacc gccagccgtg atgtgccaca tctcccacct ctatccgtcc ggcgcctcgc
     8461 tctacttcac cttcgtcgcc gggcagcgcc gaggcgagga gatcgcccag tggcgcgccg
     8521 ccaagaccgc agcctgcgac gccatcgccg cgacgggggg gacgatcacc caccaccatg
     8581 cggtcggaac cgatcaccgc gcctggatgg cgagcgaggt cggcgagctg ggcgtcgacg
     8641 tgctgcgcgc cgtcaaggcc cgcgtcgatc cggccgggat catgaacccc ggcaagctgc
     8701 tgccctgagc cggcgcgtcc ggctgcgctc gcgcgctagg gctgagcgcc ctgaccggcg
     8761 ccgccgccgt cgccctgggg cggacggatc gagctgacgc gggtgcgcag gatcgactcc
     8821 ccggagtgat cggcgttgcg gtcgacggtc tcgcgcgaga tctcgaggaa gcggtagtcg
     8881 cggtagtcgt cggggagctt cgcgcgtccc gcgaaggcgc cttggcggtc ggtcacctgc
     8941 gcgccgagcg acacggcgtc ctccggcgag ttgtagagcc agacctcata ggcctggttc
     9001 tgctcggtcg gcggaaggcg cgcctcgacc gcaagctcga actgctcgcc acgacgagtg
     9061 atgaccgcga ggccggcgtg gcggttgcgg ttcgcgccgc tgtccggcac cgcgcgcaac
     9121 acgccctggc caatgagctc ggtctgcatg ccggaggtgc tgtcgccgcc gtcgtcggag
     9181 tcgtcgccgg tcagcaggcc gactggccag accagcacga tgagcagcag cgccacggcg
     9241 gtcgccgcgg ctgccgcaag acggcggcgg cgcacgactg cggccgcctc gggactcagc
     9301 tccctgcgcg gggtggcggc cttcggctcg cccgccttgc cgggcggctt ggacttggcg
     9361 ggaccgccgt cgctcgatgg gatcgtcggc agctcgccgt tcgggtagag gtgctcgagc
     9421 gagtcgagga ccgagcgtga ccaggcacgc gcgggctcgg agcggcgcag gtggccgcgc
     9481 gtggcggtcg cctgcttggg gtcctgctgc ccgagcaggt agtcggcgat ctcgctgcgc
     9541 cagtcgtcgt cgacggctcg cgcgctgacc ggcgcgaggc tggtgagcgc ctcgccggcg
     9601 agctcgcgga cgcggggctc gggcatcccg agcgtgtcgg cgagttggtc gtagctctgg
     9661 ccccgcttga ggatcaggtc gacgatcgcc cgctgctgcg ccgacagctg gtcgtaggag
     9721 gccatgcggg cgggaacgat agcctgcgcc cgtgactgac gaggcagccg cggcggcgca
     9781 gacgcccggc cagacgctca acgacgtgct cgggatcgag gtcggcgagt tcgacgagca
     9841 aagcatcacc ggcggcttcg aggtggccga ccgcgtgcgc cagccgttcg gcatcgtcca
     9901 cggcggcgcc tacgccgcgg ccgccgagac gttggcgtcg gtggcgacgt tctacgcggt
     9961 caacgccaac ggcgagatcg cggtcggcca gaacaacgac acgagcttcc tgcggccggt
    10021 ctcggccggc cgcgtccacg ccgtcggaac ctgccgccac cgcggtcgca cgagctggat
    10081 ctgggacttc gagttcacgg acgaccagga ccgcgtctgc gccatctcgc gcgtcacgat
    10141 cgcggtgcga ccggcgcctt cttagaccgg ccataccgat tcgttatggg gaggcggcgg
    10201 tcgcggccga aggcgcgggt cgagatccgg acgccgggcg gggcttggcg gcgcttgtgc
    10261 tcggcggcgt cgacgagcgc gatcacgcgc gccacctcct cggccggata gccgcgcgcg
    10321 accaggccgg caggatcgag gtcctgctcg acgtagcccg cgaggatcgc gtcgagcagc
    10381 tcgtaggggg ggagggagtc ctcgtcgcgc tggtcggcgc gcagctccgc cgagggcgct
    10441 cgctcgatca cgctgctcgg gatcagctcc gtcccggcct gcgcgttgcg ccagcggacg
    10501 agccggtaga cccagccctt gaagacgtcc ttgagcactg cgaagccacc ggccatatcg
    10561 ccgtagaggg tcgagtagcc gaccgacagc tccgacttgt tgccggtcgt caggacgagc
    10621 caaccgaact tgttcgacag cgccatcagg aggttgccgc ggatccgcgc ctggaggttc
    10681 tcctcggtca ggtccggctc gtggcccgcg aagacctcgg cgagcagctc ctcatagacc
    10741 tccatggcgg cgccgatcgg cagctcgtag agccccgcgc cgaggttcac ggcgatcgtg
    10801 cgcgcgtcgg cctgggtcgc gtcggaggag tggggggagg gcatgacgac cgtcgtcacc
    10861 cgctcggcgc cgagcgcgtc gacggcgacc agcgcggcga gcgccgagtc gatcccgccc
    10921 gagaggccga gcaccgctcc gccgaaaccg ttcttgtcga cgtagtcacg cacgccggtc
    10981 acgagcgccg cgtagacctc gtcctcgggg tcgagcagct cggcgcgtgg cgccggcagg
    11041 acgtcggagc cgggcatcgt cgggcggctc gaggcgcgca gcgagccgag ccggacgacg
    11101 gggacggagt gtcccgagcg cgcgcggcgg gcggcctcgc ggtggcgcgg gtcgcgcagg
    11161 cgaccggccg cgatcggccc gacgtcgagc gagcagacgg tgatcgactc ctcgaactgg
    11221 ggcgcccgcg cgacgatcgc gccgctgtgg tcgatcgcca ggctgtggcc gtcgaagacg
    11281 agctcgtcct ggccgccgac ggcgttgcag aacagcaccg acacgaggct gtcgcgcgcc
    11341 cgctgggcga gcatccgctc gcgctgcagc cccttgccgt ggtggtaggg agacgccgac
    11401 aggttgacga cgacctgggc cccagcgagc gcctcggcgg tcgccggggg gcccggctcc
    11461 cagatgtcct cgcagaccgt gatgccgacc gtcacgccgt tgacctcgac ggtcgcgggc
    11521 gtctcaccgg cctggaagta gcgctgctcg tcgaacaccc cgtagttcgg caggcgcagc
    11581 ttgcggtaga cgcccgccac gcgcccgtcg gcgagcaccg ccgccgcgtt gtagacgtcg
    11641 tcggcgcgct cggggaagcc gacgaccgcg acgacgtcga tcgtctgcgc cgccagctgc
    11701 tcgagcgcgg ccgccgcctc gtcgaggaag tgcggccgca gcagcaggtc ctcgggcggg
    11761 tagccggtca gtgcgagctc cgggaagacg accagcgccg caccgcgctc gcgtgcctgg
    11821 acggtcgccg cggcgatccg gtcggcgttg ccgtcgatgt cgccgacggt cgagttgatc
    11881 tgcgccagcg cgcagcgcag cggctccggg cgcggggaca tggccgaagt atggctcagc
    11941 gctaggcggt taccgcggcc acggtcgccg ccgtgcagct cgccagtcgc tctggctcga
    12001 gctcgaccag cgtatggccg tcgccgcccg ccgcccagac gctcgcgtgc tcgagcagcg
    12061 ccgcgtcgag gacgaccggc agtccgtggc cgagcggcgg caccccggac gcggggaagc
    12121 cggtcgcggc gcgcgtctcg ctgccgctcg cgggccgcac ctcggcgaca tcgaggacgt
    12181 cggcgagcgc ctcggtgtcg acgacgcggt ccgcggaggc gacgcagacg acgggatcgc
    12241 cgtcggcgac gaagaccgcg cagctgacga tccgcgccgc gccacagccg accgcggcgg
    12301 ccgcgtcggc gaccgacgca cgcgtgtcgg gcaggcgctg gacattgacg tcgagcccga
    12361 gatcgcgtgc gctgtcgatt accttggatc gcattcgagg tggcgccggg ccagcggagc
    12421 ctccaggagt tctataccag cacgacggcc gatgaccctt atccggtctg accaaagtcg
    12481 gcccggccct tgagccggaa cggcgtggct acttcgagcg cgcgacggcg tcggaggcgt
    12541 gcagctgctc ggcgggcgcg tccgggggcg gcgagctgag cgtccttccc gcggcctcgg
    12601 cgagctcggc gagcgactcg tggagatcgc gtgcaaggtc gtcgaggtcg tacatcacgt
    12661 cgtagtcgcc gacgaggccg aagtccatcc ggccgtcgta gctcatgatc gcgatgccga
    12721 cgccctggtt cttggcgagc ggcacgagcg ggaacgggtc gagagcgcgc cggccgagca
    12781 ggtagagcgg gatctgcggg ccagggacgt tggtgatcac gaggttgaag aagcgctgcc
    12841 tgctcatcag gcgcgccgcc tgacccatga tcgtcggcgg ggcgaagccg ctcatctcgg
    12901 tcagggcctg cgcgccgacc gcctggccgc cgtccttgag gtggtccatc gcctggcgca
    12961 cgatctgctg acgcgcgacc gggtcctggc accagaccgg gagcggcgcc atcatcgcgg
    13021 cgacctggtt gccgagctcg ccgacctgct cctgcgagcg cacgctgacc ggcaccatcg
    13081 ccttgagctc gagcccctcg gtcgagacgc cgcggcggcg caggtggcga ccgagcgcac
    13141 cggcgaccgc ggtcagcacg acgtcgttga ccgtgccgcc gagctcgttc ttgatcgcct
    13201 tgacgtcggc gaggttcgcg cgcacccagg tgaagcggcg gtgcggcccg atcggccggt
    13261 tgtacggcgt ggtcggggct gggctgagcc ccgcccaggc catcgcgccg aggccggcgg
    13321 cggtgttgac cgcctcgcgc gcgatccgcc gcggggcgcg gaagaccgcg cgcagcgtgc
    13381 ggccggtctc cgccgggatc gtcgcccgct cgacgagcgc ctcgccgagc agctgcgcct
    13441 ggctcggcag cggacgcggc agccacgggg tgccggggtc ggcaggcgcg accggctcgg
    13501 gctgggtgtc gaacagcacg gtgacgatgt cgacgcccga gatgccgtcg acgagcgcgt
    13561 ggtgggtctt tgacagcagc gcgaagcggt cgccctcgag cccgtcgacc aggaagagct
    13621 cccagagcgg cttgtcgcga tcgagcggct gcgcgaagac gcgtccggcg agggcgcgca
    13681 gctgctgctc gctgcccggc gcgggaaggc cgctgtggcg caggtggtag cgcaggttga
    13741 gatgcgggtc gtcgacccag cgcggccgtc cctgcccgag cgggacgaac gcaagccgct
    13801 ggcggaagcg cgggacgagg tgcagccgcc gctcgacgtg ctcgagtagc tcgacgtact
    13861 cgggcgcctt gccctcgaag acgatcaccg acgccacgtg catgtgcgcg gagtcgtcct
    13921 ccaggtggag gaaggacgcg tcgagtccgc tcagtcgatc gccctgcatc gcgcccgaca
    13981 ctctagagcg tgagcgcgag ctgtccgggg ggcggggcgg ccgcttgcgg atcgcgccgc
    14041 tcctccatcg ccgcgacccg cacgccgagc aggcggaccg gccgcgccgg gtcgagcgcc
    14101 cgcagcagct cgagcgcgac gccgtgcacg gtggcgaagt cgtttgcggg gcgctcgacg
    14161 gtgcgcgccc gggtcagcgt cgagaagtcg tcgagtcgga ccttgatccc aatcgtgcgt
    14221 ccgctgcggc cgtgctcggc gagctcctca gagagctggc gcgcgagccg ctcgagcacc
    14281 ggttccagct ccgccaggcc gctcaggtca cggtcgaacg tcgtctcgcg cgactccgac
    14341 ttgcgcacgc gctcggtctc gagctgtcgc tcgtcctcga accgcgccag gcgcgccagc
    14401 tgcggcccca ggcggccgcc gaaccactcg ccgagcgtcg cgtcgggcgt tgccgcgagc
    14461 tggtcgagcc ggtcgatgcc ccgcgccgac agccgctcga ccgtcttcgg cccgatcccc
    14521 gggatcagcg cgggggaggc gcccgcgaag cgctcgcgcg cctcgtcgag cgtcagcgcg
    14581 aggaacccgt tcggcttgtc ggcgtcggag gcgaccttcg cgaccagctt gttcggcccg
    14641 atcccgatcg agcaggcgag gccggtcgcc tcggtcacgg cccgcttcag ccgccgtgcc
    14701 gccgcgcgcc agcgttcgat cccggtgaag tcgaggtagg cctcgtcgag gccaacctgc
    14761 tcgacgcgct cgacgtgtgc gtgcagcacc tccatcaccg cgcgcgagcg cgagcggtag
    14821 tagtcgaagt cgggcggcac gaacagcgcc tgcggacaca gccgccgtgc gcgcgccgcc
    14881 ggcgtcgccg agaagacgcc gaagcggcgg gcctcgtagc tcgccgtcgt gacgacggcg
    14941 cgcgggccgc tgccggcgac cacgaccggc ttcccgcgca gctccggccg tcgctgcagc
    15001 tcgcagttga cgtagaaggc gtccatgtcg aggtgcgcga tgcggcggtc gccgtcgtcg
    15061 gacatcaccc ggccacgata gggcgagccc acgtcggacc gggataggct ggccgggatg
    15121 gccggaggct ccaagccttg cccgacggct gaaactcaat gctcgataac ggggattatg
    15181 tgaacccgga tcgagaacga tgatcgccgc gtcggcagcc gagcacatcg agctggtcgt
    15241 cttcggcctg ctcgtcgcgg tctccgccct cgcggtcgtg gcgcggctcg tgcggatccc
    15301 gtacccgatc ctgctcgtga tcggcggcgt cgcgatcggg ttcgtgcccg agatcccgaa
    15361 catcgagctc gatcccgaca tcgtcctgct ggtattcctg ccgccgctgc tctacgcggc
    15421 ggcgttcttc tcgaacctgc gagagctgcg cgccaacgtg cggccgatcg gcctgctggc
    15481 gatcggcctg gtcatcgtca cgacgctcgc ggtcgccgcg gtcgcccact gggcgatcgg
    15541 cctcgagtgg caggtcgcct tcgtgctcgg ggcgatcgtc tcgccgaccg acgccgtcgc
    15601 gccggcgacg atcctgcgcc ggctcggcgt cccgcggcgc gtcgtgacga tcgtcgaggg
    15661 cgagaacctg accaacgact ggaccgcgct ggtcacctac aagttcgccg tcgctgcggt
    15721 cgtcacgggc agcttcacgc tcgtcgacgc cggccccgac ttcatcctga ccggcgtcgg
    15781 cggcgcggcg atcggcgtcg ccggcggcct ggcgatcgcg gcagtgcgcc ggcggctcga
    15841 cgacccgccg accgagataa cgatctccct gctgaccccg tacgcggtct atttgccagc
    15901 cgaggagctc ggcgtctccg gcgtgatcgc cgcggtgacg gtcggcgtgc tgctcggctg
    15961 gcgcgcctcc gagctgacga ctcacacgac ccggctgcag tcctacgcga tctgggagat
    16021 cctgcagttc ctgctcaacg ctgtgctgtt cttgctaatc gggctgcagc tgccgaccgc
    16081 cctcgacgca ctcgacgagc gcaacggcgg cgagctgctc ggctatgcgg tgctcgtctc
    16141 ggcggtcgcg atcctcgtgc ggatcgcctg ggtctacgcg ctcagcgccg tcgaccgcaa
    16201 cgtccgcagc gagatctccg gccctgacga cggcgcggcc tggaaggagg tcacgctcgt
    16261 cgcatggagc ggcatgcgcg gcggcgtgtc gctggcggcc gcgctcgcga tcccgctgca
    16321 gaccgacacc ggcgccgggt tcccagagcg cgacctagtc ctcttcctga ccttctcggt
    16381 gatcatcgcg acgctggtcc tccagggcct cgcgttcccg ctgctgatcc gcgcgctcga
    16441 cctcgacgag gacagctccg acgccgacga ggagctgacc gcgcggatcg agaccgcgta
    16501 tgcggcgctt gaccggatcg aggagctgac cgaggaggag tgggtgccgc gcgacaccct
    16561 cgcgcgcgtc cgcgacctct acgactaccg ccgccggcgc ttcagctcgc gcctcgacga
    16621 tcgcgatcct gaggacggct tcgactatga gcagcgcgcc gagctctacc ggcgcgtgat
    16681 gaacgaggtc attgacgccc agcgggcggc gctgcgccgc ctgcgcgacg acggcgagat
    16741 caccgacgag gtgcggcgca acgtcgagca cgagctcgac ctcgagcagt cgcggctcga
    16801 gcgctccgac agctcgactc agccgcgctc ggccaccatc agcgcctgag tcgcgcgacc
    16861 gatcatgccg cgctcgtcgt gcagcgcgct gtcggacagg ccgatgccgc tcgcctctgg
    16921 gtaggtcacc gcgtcgaggc agacccactc cccctccggc aggcgccgca agttcactgt
    16981 caggtcggtg tttatgaaga tgtagcggcg gtagtcgagc ggcgcgctga cgccgttgcc
    17041 ggagtccgca gcgaccagca cgcgatcgcg cggcagcggc tcctcgccgt cgatcagcgg
    17101 cacgcgcatc cgcatccagg cccgtgccgg accggggtcg acgaagcctc cctcgaccaa
    17161 gcggatctcc atcgccgagt ggtagccgac gtcccagggc accgggaaga agctctgcgt
    17221 agccgcgttc gccggcccgg ggatgcgcgc cggctccggc ggcgggcggt cgatctcgac
    17281 ctcggcggtg cggatccgcc aggcgcgagc gcggatcact gcttcgccgt cgtgcagcag
    17341 cgtgccctcg accagctcga cgctgcgacc cggacgcacg acctccgcga cggtcgtcag
    17401 gcgcgcgatc ggcaccggtc gcaggatctc gtaggtgatc cgggcgagct gcccgtgctc
    17461 gagccgaccc gccgagtcga tctcccggcc cagcagcgca cacggcggac cggcatgctg
    17521 gctgccctcg tcccacggcc cgcgcgtcag ctccgtcgcg gcgaagccca ccccgtccgc
    17581 cacatagaag gactcggcca cgccgggatc ctatgaccac cggcctccgg ccacggatcg
    17641 gcgggggtct ggggcgtgag cccccagggg ttatccccgc gggggtctgg gggcgtgagc
    17701 cccc
//
LOCUS       DATABV010000065         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1867272, whole genome shotgun sequence.
ACCESSION   DATABV010000065 DATABV010000000
VERSION     DATABV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867272"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="VHF88_02655"
     CDS             <1..1807
                     /locus_tag="VHF88_02655"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX2160698.1"
                     /translation="VAMASDIRVILIKLADRLHNMRTIDAMPKQKQHDKARETLDIFA
                     PLAHRLGIHAIKWELEDLAFATLHPRKFTEIKGLVNQQRDEREGYVARAGEYLGKELE
                     AVGIDAQISGRAKHFYSIYSKMTKKDREFNEIYDLTAMRVLVGSVKDCYGAIGVIHSL
                     WKPLPGRFKDYVAMPKFNMYQALHTTVIGPEGRPLEIQIRTQEMHDTAEYGIAAHWIY
                     KDGKRRPEDKVEWLKQLVDWQQELQDPQEFAQTLKADLFEDEVFVFTPKGEVKSLASG
                     ATPLDFAYSVHTDVGHRCVGAKVNGKIVPLHYELQSGDICEVLTSKKERGPSRDWLSL
                     AKTTRAQSKIRAWFKRERREDSERTGREILQENLRRQGLPPQKIAGSPLLADVIREMG
                     FRKGDDFYVALGQAKISAKVTTQKIMQKLRQGESAVDHDGAAAQLLDKKRERSRSTGN
                     ASAYGIVVDGIDDVMLRLAKCCRPVPGDPIVGYISLGKGITIHHEACSNAIALQRNPE
                     RFTKVWWEGDTAGSSFRAEIEIDGYDRHRLLEDLSRCFAEAGVNILEARCTTVHPMIK
                     NRFVVEVGDTAALKSCIQRLRNIDGVFDAYRVTPA"
     gene            complement(1814..2755)
                     /locus_tag="VHF88_02660"
     CDS             complement(1814..2755)
                     /locus_tag="VHF88_02660"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2160699.1"
                     /translation="MLPSRAKKKRMLVIVNPYATTVSDRLRNLVVYALQARYEVQAVD
                     TERREHATQICREAAREGYDVVVAFGGDGTVNEAANGLLGSDTPLSCLPGGATNVFHR
                     TIGIPGDVVDATEHLLALADDFRPRKVDLGLVNGRAFVFSSGVGLDASVVERVDAHPR
                     LKARLGPYYYSWAAVSTFNRRYLVKPPRMRVTVAGETVDAVTVIAQNSDPFTYFGPRP
                     IRVCEGAGLDTGSLGVAVLRKATPLELPTLIPRLFSGSARTVSKHRQITPFTDVAEAL
                     VESADGRPLPVQVDGDYIGEQQSIRFSAAPRALTVVS"
     gene            complement(2755..>3757)
                     /locus_tag="VHF88_02665"
     CDS             complement(2755..>3757)
                     /locus_tag="VHF88_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2160700.1"
                     /translation="RVSDAPDPFAGATAAGIHRLSIPTPFAVGRVNCYLIDDEPLTLV
                     DAGPNSGTSFDELTQGLAALGRSLEEIELIVITHQHIDHLGLVGLVAARSGADVATID
                     HAVPMVENFAEEMQADDDFATATMLRHGIPEDVVQALQSVSRAFRAWGAAAKVTRVLH
                     DGDELSFRNRTWKVHWRPGHSPTDTVFEDAERRTLIAADHLLGHISSNPLITRPLDGG
                     SERPQSLVTYLDSLAKTRAMDVDLVLPGHGDPVVDHRALIDERFALHRRRADKLHRLV
                     HERPRSAYELAQALWGNIAVTQAYLTLSEILGHTDLLLNEGRVRELEDGDTVRFEAT"
BASE COUNT          630 a         1262 c         1318 g          547 t
ORIGIN      
        1 ggtcgcgatg gcctcggaca tccgggtcat cctgatcaag ctcgccgacc ggctgcacaa
       61 catgcgcacg atcgacgcga tgccgaagca gaagcagcac gacaaggcac gcgagacgct
      121 cgacatcttc gccccgctgg cgcaccggct cggcatccac gcgatcaagt gggagctcga
      181 ggacctcgcc ttcgcgaccc tgcacccgcg caagttcacc gagatcaagg gcctcgtcaa
      241 ccagcagcgc gacgagcgcg agggctacgt cgcgcgcgcc ggcgagtacc tcggcaagga
      301 gctcgaggcg gtcggcatcg acgcgcagat ctccggccgc gcgaagcact tctactcgat
      361 ctactcgaag atgaccaaga aggaccgcga gttcaacgag atctacgacc tcacggcgat
      421 gcgcgtcctg gtcggctcgg tcaaggactg ctatggcgcg atcggcgtca tccactcgct
      481 ctggaagccg ttgcccggcc gcttcaagga ctacgtggcg atgccgaagt tcaacatgta
      541 ccaggcgctc cacacgacgg tgatcggacc cgagggacgg ccgctggaga tccagatccg
      601 cacccaggag atgcacgaca ccgccgagta cggcatcgcg gcgcactgga tctacaagga
      661 cggcaagcgc cggcctgagg acaaggtcga gtggctcaag cagctcgtcg actggcagca
      721 agagctgcag gacccacagg agttcgcgca gacgctgaag gccgaccttt tcgaggacga
      781 ggtcttcgtc ttcacgccga agggcgaggt caagtcgctc gcgtccggcg cgacgccgct
      841 cgacttcgcc tactccgtcc acaccgacgt cgggcaccgc tgcgtcggcg ccaaggtcaa
      901 cggcaagatc gtgccgctgc actacgagct gcagtccggc gacatctgcg aggtgctgac
      961 ctcgaagaag gagcgcggcc cgtcgcgcga ctggctctcg ctggccaaga cgacgcgtgc
     1021 gcagtcgaag atccgcgcct ggttcaagcg cgagcgccgc gaggactcag agcgcaccgg
     1081 tcgcgagatc ctgcaggaga acctgcgccg ccagggcctg ccgccgcaga agatcgccgg
     1141 ttcgccgctg ctcgccgacg tgatccgcga gatgggcttc cgcaagggcg acgacttcta
     1201 cgtcgcgctc ggccaggcga agatctccgc caaggtcacg acacagaaga tcatgcagaa
     1261 gctgcgccag ggcgagtccg ccgtcgacca tgatggtgct gccgcccagc tgctcgacaa
     1321 gaagagggag cgcagccgct cgaccggcaa cgcgtccgcc tatggcatcg tcgtagacgg
     1381 catcgacgac gtgatgctgc gcctggcgaa gtgctgccgc ccggtcccgg gagacccgat
     1441 cgtcggctac atctcgctcg gcaaggggat cacgatccac cacgaggcgt gctcgaacgc
     1501 catcgccctg cagcgcaacc ccgagcgctt caccaaggtc tggtgggagg gcgacaccgc
     1561 cggctcgtcg ttccgcgccg agatcgagat tgacggctac gaccgccacc gcctactcga
     1621 ggacctgtcg cgctgcttcg ccgaggccgg cgtgaacatc ctcgaggcgc gctgcacgac
     1681 ggtccacccg atgatcaaga accgcttcgt cgtagaggtc ggcgacaccg ccgcgctaaa
     1741 gagctgcatc cagcgcctgc gcaacatcga cggcgtattc gacgcctaca gagtgacgcc
     1801 ggcctagttc gtctcaggag acgaccgtga gggcgcgggg ggctgcgctg aagcggatgg
     1861 actgctgctc gccgatgtaa tcgccgtcaa cctggaccgg cagcggacgg ccgtcggcgc
     1921 tctcgacgag cgcctccgcg acgtcggtga agggcgtgat ctggcggtgc ttcgagacgg
     1981 tccgcgcgct tcccgagaac aggcgcggga tcagcgtcgg cagctcgagc ggcgtcgcct
     2041 tgcgcaggac ggcgacgccg agcgacccgg tgtcgagccc ggcgccctcg cagacccgga
     2101 tcggccgcgg tccgaagtag gtgaaggggt cggagttctg ggcgatgacc gtgacggcgt
     2161 cgacggtctc gccggcgacg gtgacgcgca tccgcggcgg cttgacgagg tagcggcggt
     2221 tgaacgtcga gaccgccgcc cacgagtagt agtaggggcc gagccgcgcc ttcagccgcg
     2281 gatgggcgtc gacgcgctcg accacgctgg catcgaggcc gacgccggac gagaacacga
     2341 acgcgcggcc gttgaccagg ccgaggtcga ccttgcgcgg gcggaagtcg tccgcgagcg
     2401 caagcaggtg ctctgtggcg tcgacgacgt cgccggggat gccgatcgtg cggtggaaga
     2461 cgttggtcgc gccgcccggc aggcagctca gcggcgtgtc ggacccgagc agcccgttgg
     2521 cggcctcgtt gaccgttccg tcgccgccga aggcgacgac gacgtcgtag ccctcgcgtg
     2581 ccgcctcgcg acagatctgg gtcgcgtgct cgcgccgctc ggtgtcgacc gcctggacct
     2641 cgtagcgggc ctgcagggcg tagacgacga ggttgcgcag cctgtccgag accgtcgtcg
     2701 cgtacggatt gacgatcacc agcatccgct tcttcttggc gcgcgagggg agcatcaggt
     2761 cgcctcgaag cgaaccgtgt cgccgtcctc cagctcgcgc acgcggccct cgttgagcag
     2821 caggtccgtg tgcccgagga tctcgcttag cgtcagatag gcctgcgtca ccgcgatgtt
     2881 gccccacagt gcctgcgcga gctcgtatgc gctgcgcgga cgctcgtgga ccagccgatg
     2941 cagcttgtcg gcgcggcgac ggtgcagcgc gaagcgctcg tcgatcaggg cgcggtggtc
     3001 gacgaccggg tcgccgtggc ccggaaggac gagatcgaca tccatcgcgc gcgtcttggc
     3061 gagcgagtca aggtaggtga ccagcgactg cgggcgctcg ctgccgccgt cgagcggccg
     3121 ggtgatcagc gggttcgagg agatgtggcc gagcagatgg tcggcggcga tcagcgttcg
     3181 ccgctcggcg tcctcgaaga ccgtgtcggt gggggagtgc cccgggcgcc agtggacctt
     3241 ccaggtgcga ttgcgaaagc tcagctcgtc gccgtcgtgg aggacccggg tcaccttcgc
     3301 cgctgcgccc caggcgcgga aggcgcgcga taccgactgc agcgcctgca cgacgtcctc
     3361 ggggatcccg tggcgcagca tcgtcgcggt cgcgaagtcg tcgtcggcct gcatctcctc
     3421 ggcgaagttc tcgaccatcg gcacggcgtg gtcgatcgtc gccacgtcgg cgcccgagcg
     3481 cgcggcgacc aagccgacga gcccgaggtg gtcgatgtgc tggtgggtga tcacgatcag
     3541 ctcgatctcc tcgagcgacc gcccgagcgc tgccagcccc tgggtcagct cgtcgaacga
     3601 ggtgccggag ttcgggccgg cgtcgacgag cgtcagcggc tcgtcgtcga tcagatagca
     3661 gttgacccgt ccgacggcga acggggtcgg gatcgacagg cggtggatcc cggcggccgt
     3721 cgcgccggcg aacgggtccg gcgcgtcgct cacgcgc
//
LOCUS       DATABV010000066         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_502002, whole genome shotgun sequence.
ACCESSION   DATABV010000066 DATABV010000000
VERSION     DATABV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502002"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /gene="hflX"
                     /locus_tag="VHF88_02670"
     CDS             complement(<1..685)
                     /gene="hflX"
                     /locus_tag="VHF88_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX2160701.1"
                     /translation="MDRGRNGRTGQSRYGAGQRTPRARQRALCIAAGPDEPDLGELRE
                     LLRTAGAAVVGEMVQRRPEPDPDRYFGKGKLGELKQAVKDADANLVACDDELLPRQER
                     NLEQAVGVPVIDRTAIILDIFADHAHSAEGMLQVELAQLEYNLARMRGLWTHLERLGA
                     GIGTRGPGESQIETDRRLARDRIAALKRRLDHQHGTRQVMRRERDRAALPTVALAGYT
                     NAGKSTLLNA"
     gene            complement(697..1866)
                     /locus_tag="VHF88_02675"
     CDS             complement(697..1866)
                     /locus_tag="VHF88_02675"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX2160702.1"
                     /translation="MKPSTRLDRLPPYLFAELERKIREKKAAGVDVISLGIGDPDTPT
                     PELIVDALAAAARDGGTHQYPSNRGRPELREAIAAFYARRFGVALDPESEIIPALGAK
                     ECVFNVNLAFLDPGSVALAADPGYPVYTGGPLLAGGEPVLMPLVPELGFVPDLDAIPA
                     DTRAAARLMYLNYPNNPTGAVAPEGFFDRVVEFARAHDVLVVHDASYTETTFDGYVAP
                     SFLETPGAKDVGVEIFSLSKGYNMTGWRTAAIVGNADAVAAYWKLKTNIDSGMFEAIQ
                     LAAVAALSEQGDRISREMSAVYARRRDLVVDALRAIGVDVRSPQGTIYIWAPVPEGHT
                     SASYCELVLEESGVVVSPGSSYGPNGEGFFRISLTVPDERLTEAMERIRSSLSSP"
     gene            complement(1863..2705)
                     /gene="dapF"
                     /locus_tag="VHF88_02680"
     CDS             complement(1863..2705)
                     /gene="dapF"
                     /locus_tag="VHF88_02680"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX2160703.1"
                     /translation="MKFEKWNALGNDYAIVEAKALPFELTPDRVRALCAQHSGVGADG
                     VLLLDRPSEAGFVASLRIFNPDGSEAELSGNGVREAVMYLRRSGWTEHDSFGIQTVAG
                     EIRPRITGPTTCTVDMGRAKLAARDFPSGSDDGRGTLNANGRDYDFQFVSIGNPQCAI
                     EWREGLEELDLPAIGPALERHELFPNRTNVSFWRRDSDSEITARIFERGVGETMSSGT
                     GATGAAVAAVLRGLDSPVTVHLDGGDLEVEVGEDLHVNLTGWAVPVYAGELSPEFLEA
                     LRDT"
     gene            complement(2716..>3317)
                     /locus_tag="VHF88_02685"
     CDS             complement(2716..>3317)
                     /locus_tag="VHF88_02685"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEX2160704.1"
                     /translation="EGLETLTGVATAEEQARLEHRLVGFVPVTQPFSVGDYMPRAHAE
                     IDAALAAGRRPIVVGGTGLYLRAALASLSLEKVPPNTADSELWSPETRHPTAIFGLTM
                     ERERLYRRIEQRVDAIVAAGAEQEVRRAQARGPSHTARKALGFQELLDGDVERMKQRS
                     RNYARRQLTWMRKTPNLRQIDMTGRTAADAADEIAERLA"
BASE COUNT          426 a         1173 c         1201 g          517 t
ORIGIN      
        1 gggcgttgag cagagtggac ttccccgcgt tggtatagcc ggcgagcgcc acggtcggaa
       61 gcgccgcgcg gtcgcgctcg cggcgcatca cctggcgcgt cccgtgctgg tgatcgagcc
      121 gccgcttgag cgccgcgatg cggtcgcggg cgaggcggcg gtcggtctcg atctggctct
      181 cgcccggacc cctggtgccg atcccggcgc cgagccgctc gaggtgggtc cagagcccgc
      241 gcatccgcgc gaggttgtac tcgagctgcg ccagctcgac ctggagcatg ccctcggcgc
      301 tgtgcgcgtg gtcggcgaag atgtcgagaa tgatcgcggt gcggtcgatc accggcacgc
      361 cgacggcctg ctccaggttg cgctcctggc gcggcagcag ctcgtcgtcg caggcgacga
      421 ggttggcgtc ggcgtccttg acggcctgct tcagctcgcc gagcttgcct ttgccgaagt
      481 agcggtcggg gtccggctcc ggccggcgct ggaccatctc gccgacgacc gccgcccccg
      541 cggtccgcag cagctcgcgc agctcgccga gatccggctc gtcaggccca gcggcgatgc
      601 agagcgctcg ctggcgggcc cgcggcgtcc gctggcccgc gccatagcgg ctctggccgg
      661 tgcgtccgtt gcgtccccta tccatccagg ctgagtctag gggctgctga gactcgagcg
      721 gattcgctcc atcgcctccg tcaggcgctc gtccggcacg gtcagcgaga tgcggaagaa
      781 gccttcgccg ttggggccgt aggagctgcc gggcgagacg acgacgccgg actcctcgag
      841 cacgagctcg cagtaggagg ccgacgtgtg gccttcgggg accggcgccc agatgtagat
      901 cgttccctgc ggcgagcgca cgtcgacacc gatcgcgcgc agcgcgtcga ccaccaggtc
      961 gcggcggcgg gcgtagaccg cgctcatctc gcgcgagatc cggtcacctt gctctgacag
     1021 cgcggcgacc gccgcgagct ggatcgcctc gaacatgccg gagtcgatgt tggtcttcag
     1081 cttccagtag gcggcgaccg cgtcggcgtt gccgacgatc gccgccgtcc gccagccggt
     1141 catgttgtag cccttggaga gcgagaagat ctcgactccg acgtccttgg cgcccggggt
     1201 ctcgaggaag ctcggcgcga cgtagccgtc gaacgtcgtc tcggtgtagg aggcgtcgtg
     1261 cacgacgagg acgtcgtgcg cgcgggcgaa ctcgacgacc cggtcgaaga acccctctgg
     1321 ggcaaccgcg ccggtcgggt tgttcgggta gttgaggtac atcagccggg cggccgcgcg
     1381 ggtgtccgcc gggatcgcat cgaggtccgg gacgaagccg agctcgggga ccagcggcat
     1441 caggacgggc tcgccgccgg cgagcagcgg gccgcccgtg tagaccgggt agccggggtc
     1501 ggcggcgagc gccacgctgc ccgggtcgag gaaggccagg ttgacgttga agacgcactc
     1561 cttggcgccg agcgcgggga tgatctcgct ctccggatcg agcgcgacgc cgaagcggcg
     1621 tgcgtagaag gcggcgatcg cctcgcgcag ctcgggccgg ccgcggttcg acgggtactg
     1681 gtgcgtgcct ccgtcccgcg ccgcggcggc gagcgcgtcg acgatcagct cgggcgtcgg
     1741 ggtgtcgggg tcgccgatcc cgaggctgat cacgtcgacg ccggcggcct tcttctcgcg
     1801 gatcttgcgc tcgagctcgg cgaacaggta cggcggcagg cggtccaggc gggtgcttgg
     1861 cttcatgtgt ctctcaatgc ctccaggaac tcggggctca gctcgcccgc atacacgggc
     1921 accgcccagc cggtcaggtt gacgtgtagg tcctccccca cctcgacttc gaggtcgccg
     1981 ccgtcgagat gaaccgtcac cgggctgtcg aggccgcgca gcactgccgc gacggccgcg
     2041 ccggtcgccc ccgtgccgga cgacatcgtc tcgccgacgc cgcgctcgaa gattcgcgcc
     2101 gtgatctcgc tgtcggaatc gcgtcgccag aacgagacgt tggtgcggtt cgggaacagc
     2161 tcgtggcgct cgagcgccgg cccgatcgcc ggcaggtcga gctcctccag tccctcccgc
     2221 cactcgatcg cgcactgcgg gttgccgatc gagacgaact ggaagtcgta gtcgcggccg
     2281 ttcgcgttca gcgttccgcg gccgtcatcg ctgccggacg ggaagtcgcg cgcggcgagc
     2341 ttcgcgcggc ccatgtcgac cgtgcaggtc gtcggcccgg tgatccgcgg ccggatctct
     2401 cccgccacgg tctgtatgcc gaagctgtcg tgctccgtcc agccggagcg gcgcaggtac
     2461 atcaccgcct cgcgcacccc gttgcccgag agctccgctt cggagccgtc cgggttgaag
     2521 atccgcagcg aggcgacgaa gccggcctcc gacggccggt cgagcagcag cacgccatcg
     2581 gctccgacgc cgctgtgctg ggcgcagagg gcgcggacgc ggtcgggcgt gagctcgaac
     2641 ggcagcgcct tcgcctcgac gatcgcgtag tcgttgccca gagcgttcca cttctcgaac
     2701 ttcaccgcgc ggactctatg cgagccgttc ggcgatctcg tcggccgcgt cggccgccgt
     2761 ccgcccggtc atgtcgatct ggcgcaggtt cggcgtcttg cgcatccagg tcagctgccg
     2821 gcgggcgtag ttgcgcgagc gctgcttcat ccgctcgacg tcaccgtcga gcagctcctg
     2881 gaacccgagc gccttgcgcg ccgtgtgcga cggcccgcgc gcctgcgcgc gccggacctc
     2941 ctgctcggca ccggccgcga cgatcgcgtc gacgcgctgc tcgatccggc ggtagaggcg
     3001 ctcgcgctcc atcgtcagcc cgaagatcgc ggtcggatgg cgcgtctctg gcgaccagag
     3061 ctcggagtcc gcggtattcg gcggcacctt ctcgagcgag aggctcgcca gcgccgcgcg
     3121 caggtagagg ccggtgccgc cgacgacgat cggtcgtcgc cccgccgcga gcgcagcgtc
     3181 gatctcggcg tgcgcccgcg gcatgtagtc gccgaccgag aacggctgcg tcaccggcac
     3241 gaagccgacc aggcggtgct ccagcctcgc ctgctcctcg gcggtggcga cgccggtcag
     3301 cgtctccagc ccctcgt
//
LOCUS       DATABV010000067         9951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2590585, whole genome shotgun sequence.
ACCESSION   DATABV010000067 DATABV010000000
VERSION     DATABV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590585"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /gene="cgtA"
                     /locus_tag="VHF88_02690"
     CDS             <1..1280
                     /gene="cgtA"
                     /locus_tag="VHF88_02690"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEX2160705.1"
                     /translation="KGGPDGGDGGEGGDVVLVCDPSRRDLSEFHRGGHYKAKRGGHGE
                     GGQRHGATAEPLELLVPPGTVAEDGERGARWELTEPGQRAVVAKGGRSGRGNKAFAGP
                     TRQTPRLAERGLPGEEGWLELRLRLLADAGLVGLPNAGKSSLLARLTAARPKVAGYPF
                     TTVSPVLGTIEADERQLVVADIPGLIEGASAGAGLGHEFLAHVERCRVLVHVLDLAPL
                     DGSDPVANHAAVEAELREHGHGLAELPRILCLSKADLVAPAAVGEAVGEWRERLGLGV
                     LSEEERWAAGEDALAGVAQPTGERVLDVVATSAATGAGLDELRAAIVRAVPEEEVALA
                     APEEVVAEHRVYRPGANDAIGIERTDAGGYRVTGMRVERLLARHDLENEEAQRYVEDR
                     LRTMGVIAALEAAGFEAGDDVEIAGMTFELDPG"
     gene            1311..2411
                     /gene="proB"
                     /locus_tag="VHF88_02695"
     CDS             1311..2411
                     /gene="proB"
                     /locus_tag="VHF88_02695"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX2160706.1"
                     /translation="MASLVVKLGSSIVADPTGEVRADVLASICDAVAQRHAVGDEVVL
                     VTSGAIARGMRLMDLSARPAAMAELQAASAVGQGKLYRTYDELLAARNVPSAQVLLTF
                     FDMSARTHYLNARHTLRKLLDWGVVPVINENDTTTTDEISFGDNDFLAAQVAILLGSD
                     RLVLLTDTDGLYSADPSRDAGAELIADVRDFEQLEALEIGFSASPLGSGGMRSKVVAA
                     EMATAAGIPATITRGTDPEAVARALAGEPAGTRFHPQRGRVSSFKLWLKYAKPTHGSL
                     AVDAGAERALRERGTSLLPVGIVDVRGEFEAGDAVEICPAGGGRRPIGKGIVNYSAAE
                     LRRIKGMKSEAVLEAIPRAAEEAVHRDYFVLD"
     gene            2588..3859
                     /locus_tag="VHF88_02700"
     CDS             2588..3859
                     /locus_tag="VHF88_02700"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX2160707.1"
                     /translation="MATTAQSVADVCRAAREASRALATASDEIKDAALEAMASALEQR
                     LGEVLEANERDMEAGAEAGLSAALLDRLKLDEARVRGIAADVRRIAALPDPVGEVLEG
                     WRLENGLEMRKLRVPLGVVAVVYEARPNVTIDCSALCLKSGNAIVLRGSSMAMHSNAV
                     LAEIAASAVESAGLPRAVVSLVAGGGREELAELATQSGVVDLIIPRGGEGLKAALQSV
                     ATVPVIYAAAGNCHVYVDASAHLDDALRIVLNAKAQRPGVCNSAETLLVHADAAPDFL
                     PRVLRELREAGVELRADARTRALAGDLADSLVDAGDDDWATEYLALILAVKVVDSIDE
                     AIDHVNRYSSGHSEAIVTDSTEAAERFTHAVDAACVYVNASTRFTDGGVFGMGAEIGN
                     STQKLHVRGPIGVRELTTYKYVAEGSGQIRE"
     gene            3856..4503
                     /gene="nadD"
                     /locus_tag="VHF88_02705"
     CDS             3856..4503
                     /gene="nadD"
                     /locus_tag="VHF88_02705"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX2160708.1"
                     /translation="MSGAAGGRRERVGILGGAFNPPHLGHLWLAQEAHARLGLDRVLL
                     VPFGEAPHRVLDADPGSRERLRMVELAAAGDERLEASAVEVDRPGPSYMADTLMLLRE
                     IEPMADLTLILGADQALRLREWHRPERVLELARVAVADRGGTGREEAVAALDGLAGAA
                     AIEGFRMPRIDVSSTLVRERVSAGLPIRYLVPDAVADHIAARGLYAAVTAAEARA"
     gene            4500..4883
                     /gene="rsfS"
                     /locus_tag="VHF88_02710"
     CDS             4500..4883
                     /gene="rsfS"
                     /locus_tag="VHF88_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX2160709.1"
                     /translation="MNAEQPATAGDAALDSAALADLIAEIASDRKAIDIRVVDVRGLV
                     SYTDYLVICSGNTERQTNAIRDAIHKQLKDDRGLLPRRAEGASEARWILLDYLDCVVH
                     VFTPDARDFYRLDKLWGDAPQRSVD"
     gene            5333..5405
                     /locus_tag="VHF88_02715"
     tRNA            5333..5405
                     /locus_tag="VHF88_02715"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5366..5368,aa:Ala,seq:ggc)
     gene            5581..6123
                     /locus_tag="VHF88_02720"
     CDS             5581..6123
                     /locus_tag="VHF88_02720"
                     /inference="COORDINATES: protein motif:HMM:NF040504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HEX2160710.1"
                     /translation="MIRYAESERDAAACAAIYRPHVEDAATSFEEISPDAADFARRIE
                     AVSTRYPWLVAERDGIVVGFAYATPHRERAAYRWTAETSVYVADGDQRHGLGGSLYRA
                     LLDLLRRQRLHVALAGITLPNAASVGLHEALGFEPVGVYRSVGYKAGAWRDVGWWQLA
                     LAGAQGAPLEPLGPQKLGDG"
     gene            6171..6590
                     /locus_tag="VHF88_02725"
     CDS             6171..6590
                     /locus_tag="VHF88_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160711.1"
                     /translation="MVASPLQPLAKAALIALMALGSVMLWIGSPIGWLWVASQMQTDS
                     KGSGFGPYMVVLFGIVVTAVVLAKGLQRLNRLYGTVSGVDDTVRIVLPWHRGLRGEQE
                     GRPQRTVLDVVMVISVAIGALAMTIWFFFFAGSSLPV"
     gene            complement(6595..7341)
                     /locus_tag="VHF88_02730"
     CDS             complement(6595..7341)
                     /locus_tag="VHF88_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160712.1"
                     /translation="MSSAIRSELLKLRTTRTFLGIVIATLGLVALIAGAQAAADPFDA
                     GATPGVDLLDVAGLAQPFALVLGILAVSTEFRHGTITPTLLTTPDRVRLMLAKLWAHG
                     AAGVVLGVVAYGIAALLMAAILSARDISSGISAGDGLEMVLGGVACIALLAAIGVGVG
                     AVVRNQVGAVIGGLAWMFMIEPLLAAIPNVGEWVEDYGIGGAITALSGSSFGGGADIS
                     QLAGGLLLVGYALLFAVAGVALLRRRDVSA"
     gene            complement(7338..8270)
                     /locus_tag="VHF88_02735"
     CDS             complement(7338..8270)
                     /locus_tag="VHF88_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2160713.1"
                     /translation="MTATEAAIEVRGLSKRFGATLAVDDLSFSVERGRIVGFLGPNGA
                     GKTTTLRALLGLVRPTAGTATIEGRAYAELEDPTGTVGTVLDGGMFHPGRSGRNHLRA
                     IALAAGVATSRVDELLELVDLGDAADRRAGGYSLGMRQRLGLAAALLGDPRVLVLDEP
                     ANGLDPQGIRWLRDLLRSLAAEGRAVLVSSHVLAEVAQTVDEVVVIHRGRSIEQAPIG
                     ELLARRAGGVRVVGPQVARLAELLRADGADVQGDGDGSIVVADRSGEQVGRVIADNAI
                     HISELASAGQTLEEVFFELTSDDLPAATETESAA"
     gene            8331..>9951
                     /gene="leuS"
                     /locus_tag="VHF88_02740"
     CDS             8331..>9951
                     /gene="leuS"
                     /locus_tag="VHF88_02740"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX2160714.1"
                     /translation="MAAESYDPREIERRWQRVWADERSWEVSNEDDGRPKSYVLEMLP
                     YPSGEPHIGHLKVYSVGDAIAHFKRRTGHRVLHPLGYDAFGLPAENHAIKTGQHPRES
                     TEASIAEFRRQFNDWGVSIDWSRELGTHEPSYYRWTQWLFLRLFEQGLAYRKQAAVNW
                     CPNDATVLANEQVVDGRCERCGALVEQRELEQWFFRITDYADRLLEDLETIDWPQNVV
                     TMQRNWIGRSEGAEVVFHCDELDIDYPVFTTRPDTLFGATFFVMAPEHPDVLRLNDSP
                     EVRDYVRRALSASAEERGEEDREKTGVALGRSVTNPVNGEQIPMYVADYVLMEYGTGA
                     IMAVPAHDERDHEFAVKHGIEVRRVIEGGEDDELPYTGDGPMVASGRFDGLHNREAFE
                     RIVAWLGDEGKGEPAVNYRLRDWLLSRQRYWGCPIPIVHCDRCGLVPVPDAQLPVVLP
                     DIDDYAPEGRSPLAAAEDWVAVDCPRCGDPARRETDTMDTFVDSSWYFLRYCDARNDS
                     APWDRDVLAGWMPVDQYIGGVEHAILHLMYAR"
BASE COUNT         1437 a         3380 c         3709 g         1425 t
ORIGIN      
        1 cgaagggggg gccggatggg ggcgatgggg gcgagggggg agatgtggtg ctggtctgcg
       61 atccgtcgcg gcgggatctg tcggagtttc atcggggtgg gcactacaag gcgaagcgtg
      121 gggggcatgg ggagggtggg cagcggcatg gggcgacggc ggagccgttg gagctgctgg
      181 tgccaccggg gaccgttgcg gaggacgggg agcggggggc tcgctgggag ctgaccgagc
      241 cggggcagcg ggctgtcgtt gcgaaggggg gacggagcgg gagggggaac aaggcctttg
      301 cggggccgac gcggcagacg ccgcggttgg cggagcgcgg gctgccgggg gaggaggggt
      361 ggctggagct gcggttgcgg ctgctggcgg atgctgggct ggtgggtttg ccgaatgcgg
      421 ggaagagctc gctgctggcg cggttgaccg cggcgcggcc gaaggtggcg gggtatccgt
      481 tcacgacggt gtcgccggtg ctggggacga tcgaggcgga cgagcggcag ctggtggttg
      541 cggacatccc gggtttgatc gagggggcga gcgctggggc cggtcttggt catgagttcc
      601 tggcgcatgt ggagcgctgt cgggtgctgg ttcacgtgct ggacttggcg ccgctggatg
      661 ggtcggaccc ggtggcgaat catgctgccg tcgaggcgga gctgcgcgag catgggcatg
      721 ggctggcgga gctgccgcgg atcctctgcc tgtcgaaggc ggatctggtg gcgcccgcgg
      781 cggtcggcga ggcggtcggc gagtggcggg agcggctcgg gctgggggtc ttgtccgagg
      841 aggagcggtg ggcggctgga gaggacgcgc tcgcgggcgt ggcgcagccg accggagagc
      901 gggtgctcga cgtcgtggcg acgtcggcgg cgaccggcgc cgggctcgac gagttgcgcg
      961 cggcgatcgt gcgggcggtt ccggaggagg aggtcgcgct ggcggcgccc gaggaggtgg
     1021 tcgccgagca tcgcgtctac cggccggggg cgaacgatgc gatcggcatc gagcggacgg
     1081 acgccggcgg ctatcgcgtc accggcatgc gggtcgagcg gctgctggcg cggcacgacc
     1141 tcgagaacga ggaggcgcaa cgctacgtcg aggaccggct caggacgatg ggcgtcatcg
     1201 ccgcactcga ggcggcggga ttcgaggccg gggacgacgt cgagatcgcg ggcatgacct
     1261 tcgagctgga cccgggctag ctcccggcag ggtttacgct gccccgcgca atggcgtcac
     1321 tggtggtgaa gctggggtcg agcatcgtgg ccgacccgac gggagaggtg cgggccgacg
     1381 tgctcgcgtc gatctgcgac gcggtcgcgc agcgccacgc cgtgggcgac gaggtagtgc
     1441 tggtgacctc cggggcgatc gcgcgcggga tgcggctgat ggacctgtcg gcgcggccgg
     1501 cggcgatggc ggagctgcag gccgcctcag ccgtcgggca gggcaagctc taccgcacct
     1561 atgacgagct gctggcggcg cgcaacgtgc cgagcgcgca ggtgctgctg actttcttcg
     1621 acatgtcggc gcggacccac tacctcaacg cgcgccacac cctgcgcaag ctgctcgact
     1681 ggggcgtcgt gccggtgatc aacgagaacg acacgacgac gaccgacgag atctcgttcg
     1741 gcgacaacga cttcctcgcc gcacaggtgg cgatcctgct cggctccgac cggctggtgc
     1801 tgctgaccga caccgacggg ctctacagcg ccgatccgag ccgcgacgcg ggagcggagc
     1861 tgatcgcgga cgtgcgcgac ttcgagcagc tagaggcgct cgagatcggc ttctcagcct
     1921 cgccgctcgg ctccggcggc atgcgctcga aggtcgtcgc cgccgagatg gcgaccgcgg
     1981 cggggatccc ggcgacgatc acgcgcggca cggatccgga ggcggtcgcc cgcgcgctcg
     2041 cgggggagcc ggcagggacg cgcttccatc cgcagcgcgg acgtgtgtcg agcttcaagc
     2101 tgtggctcaa gtacgccaag ccgacgcacg gctcgctcgc cgtcgacgcc ggcgccgagc
     2161 gggcgctgcg cgagcgcggc accagcctgc tgccggtcgg catcgtcgac gtccgcggtg
     2221 agttcgaggc gggcgacgcc gtcgagatct gcccggcagg cggcgggcgg cggccgatcg
     2281 gcaagggaat cgtcaactac tcggcggcgg agctgcgccg gatcaaggga atgaagtccg
     2341 aagcggtcct ggaggcgatc ccgcgagcgg ccgaggaggc agttcaccgc gactacttcg
     2401 tcttggattg acaccggggg ctcgcgccac cggacccccg tggcgcctgg ctggcggctg
     2461 aagggactcg cgtcccccgg agcccccgcg cgcggcaggt ctaccgacgg ctcccgcgga
     2521 gggatccctt ctccttcggc tcccgtggcg atctgggacg ggtggacccg tcggttaccc
     2581 tgcctgtatg gcaaccaccg cccagtcggt agccgatgtc tgccgtgcgg cgcgcgaggc
     2641 gtcgcgggcg ctggccaccg cgagcgacga gattaaggac gcggcgctcg aggcgatggc
     2701 gagcgcgctc gagcagcggc tcggcgaggt gctcgaggcg aacgagcgcg acatggaggc
     2761 cggcgccgag gcggggttgt cggcggcgct gctcgaccgg ttgaagctcg acgaggcgcg
     2821 cgtgcgggga atcgccgcgg acgtgcgccg gatcgccgca ttgcccgatc ccgtcggcga
     2881 ggtgctcgag ggctggcgct tggagaacgg cttggagatg cgcaagctcc gcgtaccgct
     2941 cggagtcgtc gcggtcgtct acgaggcacg accgaatgtg acgatcgact gctcggcgct
     3001 ctgcctgaag tccggcaatg caatcgtgct gcgcgggtcg tcgatggcga tgcactcaaa
     3061 cgccgtgctc gccgagatcg ccgccagcgc ggtggagtcg gcggggctgc cgcgcgccgt
     3121 ggtatcgctg gtcgccggcg gcgggcgcga ggagctggcc gagctggcga cccagtccgg
     3181 tgtcgtggac ctgatcatcc cgcgcggcgg cgagggcctg aaggccgcgc tgcagagcgt
     3241 ggcgacggtc cccgtgatct atgcggccgc cggcaactgc cacgtctacg tcgacgcgag
     3301 cgcccacctc gacgacgcgc tgcggatcgt cctgaacgcc aaggctcagc ggcccggcgt
     3361 ctgcaactcc gccgagacgc tgctcgttca cgccgacgcg gcgccggact tcctaccgcg
     3421 cgtcctgcgc gagctgcgcg aggccggcgt cgagctgcgc gccgacgcgc gcacacgcgc
     3481 gctcgccggc gacctcgccg actcgctcgt cgatgccggc gatgacgact gggcgacgga
     3541 gtacctggcg ctgatcctcg ccgtcaaggt cgtcgactcg atcgacgagg cgatcgacca
     3601 cgtgaaccgc tacagctccg gccactcgga ggcgatcgtc accgactcga cggaggcggc
     3661 cgagcgcttc acccacgccg tagacgcggc ctgcgtctac gtgaacgcct ccacccgctt
     3721 caccgacggt ggcgtcttcg ggatgggcgc cgagatcggc aactcgacgc agaagctgca
     3781 cgtgcgcggg ccgatcggcg tgcgcgagct gacgacctac aagtacgtgg ccgagggcag
     3841 cggccagatc cgcgagtgag tggcgccgcg ggcggccggc gcgagcgcgt cgggatcctc
     3901 ggcggggcgt tcaacccgcc gcacctcggt cacctctggc tggcgcagga ggcgcacgcg
     3961 cggctggggc tcgaccgcgt gctgctggtg cccttcggtg aggccccgca ccgcgtgctc
     4021 gacgccgacc cgggttcccg cgagcggctg cgcatggtgg agctcgcggc ggccggcgat
     4081 gagcggctcg aggcgtcggc cgtcgaggtc gaccggcccg gtccgtccta catggccgac
     4141 acactgatgt tgttgcgcga gatcgagcca atggcggacc tgacgctgat cctgggggcc
     4201 gaccaggcgc tgcggctgcg cgaatggcac cggccggagc gggtgctcga gctggcgcgg
     4261 gtcgccgtcg cggaccgcgg cggcaccggc cgcgaggagg ccgtcgcggc gctcgacggc
     4321 ctggcaggcg ccgcggcgat cgaaggcttc cgcatgccgc ggatcgacgt cagctcgacc
     4381 ctcgtgcgcg aacgcgtgtc cgccggcctg ccgatccgct atctggtccc cgacgctgtc
     4441 gccgaccaca tcgcggctcg cggcctctac gccgccgtga ccgccgccga ggcgcgcgca
     4501 tgaacgccga gcagcccgcc accgccggtg acgccgccct cgactcggcc gcgctggccg
     4561 acttgatcgc cgagatcgcc tcggaccgca aggcgatcga catacgcgtc gtcgacgtcc
     4621 gcgggctcgt cagctacacc gactatctcg tgatctgctc cggcaacacc gagcgccaga
     4681 ccaacgcgat ccgcgacgcg atccacaagc agctcaagga cgaccgcggc ctgctcccac
     4741 gccgcgccga gggcgccagc gaggcacgct ggatcctgct cgactacctc gactgcgtcg
     4801 tccacgtctt cacgcccgac gcgcgcgact tctaccgcct cgacaagctc tggggcgacg
     4861 ccccgcagcg cagcgtcgac tagccggttc cgtcctgcac atatgcgatg cttgccctgt
     4921 gctgagctcc aacttgaaag ggtccatcgc ggagatgaag atcgccacgg cggccgtgga
     4981 gctcgggctc acggtcttga cacctgtggt cgagcacagc cggtacgaca tggcgttcga
     5041 tatcgctggt cgcctgcaca aggtgcagtg caagtggggc gcctatgacg ccgcaagagg
     5101 cgtcgtgaag gtgaagctgc agagccagcg ccacactccc aatggctacg tccggacgac
     5161 ctacaccgag gatgagatcg atctcgtagc cgcctactgc ggtgatctgg agcgctgcta
     5221 tctgctgccg caacacctgg ccgttcgcag acgggagata tggcttcgcc tgacggcgcc
     5281 ggcgaatgca cagcgcgcgt gtattaacct tgctgccgac ttcgagtttc ctggggctgt
     5341 agctcagctg gaagagcgcg tcgctggcag cgacgaggtc aggggttcga gccccctcag
     5401 ctccacttct accgcgtctc cgcttgtgcg gcggatcggg tcacacgagt tccgcaatcg
     5461 gttcggctac cacctcgagc aggcggccgc cggtaacagc gtggtgatca cgcgtcacgg
     5521 taggccgttc gcggagcttc gctcggcaga gggtgcgaac gacgatgctg cgtcggcgct
     5581 gtgatccggt acgcggagtc ggagcgcgac gcggctgcgt gcgccgcgat ctatcgaccg
     5641 catgtagagg atgccgcgac gtcgttcgag gagatctcac cggatgcggc tgacttcgcc
     5701 cggcggatcg aggcggtgtc gacgaggtat ccgtggctgg tcgccgagcg ggacgggatc
     5761 gtcgtggggt ttgcgtacgc gacgccgcac cgcgagcggg cggcgtatcg ctggaccgcc
     5821 gagaccagcg tctacgtggc cgatggcgac cagcggcacg ggctcggagg gtcgctctac
     5881 cgggcgttgc tcgacctcct gcggcgtcag cggctgcatg tggcgctagc cgggatcacg
     5941 ctgccgaacg cggcgagcgt cggactacac gaggcgctcg gcttcgagcc ggtgggggtg
     6001 tatcggagcg tcggatacaa ggcgggggca tggcgcgacg tgggctggtg gcagctggcg
     6061 ctcgccggcg cgcagggtgc gcccttggag ccgctgggac cgcagaagct cggcgacggc
     6121 tagcgccgca aggccgctcc agctcaaggg aggcatgtca gacttcggag atggtcgcgt
     6181 ctccgttgca gccgctcgcc aaggcggcgc tgatcgcgct gatggcgctc ggcagcgtca
     6241 tgctctggat cggctcgccg atcggctggc tctgggtcgc ctcgcagatg cagaccgaca
     6301 gcaaggggtc tggcttcggg ccctacatgg tcgtgctgtt cgggatcgtc gtcaccgccg
     6361 tcgtcctcgc caagggcctg caacggctca accgcctcta cgggacggtc tcaggagtcg
     6421 acgacacggt gcggatcgtc ctgccctggc accgcgggtt gcgcggcgag caggagggca
     6481 ggccgcagcg gaccgtgctc gacgtcgtga tggtgatcag cgtcgcgatc ggcgccctcg
     6541 cgatgacgat ctggttcttc ttcttcgccg gctcgtcgct gccggtctga gggctcaggc
     6601 gctcacgtcg cggcggcgca gcagcgccac cccggcgacc gcgaacagca gggcatagcc
     6661 gaccagtagc aggccgccgg cgagctggct gatatctgcg ccgccgccga agctgctgcc
     6721 gctgagggcg gtgatcgcgc cgccgatgcc gtagtcctcg acccactcgc cgacattcgg
     6781 gatcgccgcg agcagcggct cgatcatgaa catccacgcc agtccgccga tcacggcgcc
     6841 gacctggttg cgcacgaccg cgccgacccc gacgccgatc gcggccagca gcgcgatgca
     6901 cgccaccccg ccgaggacca tctccaggcc gtcaccggcc gagatcccgg acgagatgtc
     6961 gcgcgcggag aggatcgccg ccatcagcaa agcggcgatg ccgtaggcga cgactcccag
     7021 gacaacgccg gcggcgccgt gagcccacag cttcgccagc atcagccgga cccggtccgg
     7081 cgtggtgagc agcgtcggcg tgatcgtccc gtgcctgaac tccgtgctca cggcgaggat
     7141 gcccagtacg agcgcgaagg gctgggcgag cccggccacg tcgagcaggt cgactcccgg
     7201 cgtcgccccg gcgtcgaagg ggtccgccgc ggcctgggca cccgcgatta gcgcgacgag
     7261 cccgagcgtc gcgatcacga tcccgaggaa cgtgcgcgtg gtgcgcagct tcagcagctc
     7321 gctgcggatc gcgctgctca cgccgcgctc tctgtctcgg tcgcggccgg caggtcgtcg
     7381 gaggtcagct cgaagaagac ctcctccagc gtctgtccgg ccgacgccag ctcggatatg
     7441 tggatcgcgt tgtcggcgat caccctgccg acctgctcgc cgctgcgatc ggccacgacg
     7501 atcgagccgt cgccgtcgcc ctgcacgtcg gcaccgtcgg cacgcagcag ctcggccagc
     7561 cgggccacct gcgggcccac gacccgcact ccaccggccc gtcgcgccag cagctcgccg
     7621 atcggagcct gctcgatcga gcggccgcgg tggatcacca ccacctcgtc gacggtctgg
     7681 gcgacctccg ccagaacgtg gctcgagacc agcaccgcgc ggccctccgc ggcgagcgag
     7741 cgcaggaggt cgcgcagcca gcggatgccc tgtgggtcga gtccgttcgc cggctcgtcg
     7801 aggaccagca cgcgcggatc gccgagcagc gccgcggcta gcccgagccg ctgccgcatg
     7861 ccgagcgagt acccgccggc acggcggtcg gccgcgtcgc cgaggtcgac gagctcgagc
     7921 agctcgtcga cgcggctcgt cgcgactccc gccgcgagcg cgatcgcgcg caggtggttg
     7981 cggccactgc gccccgggtg gaacatcccg ccgtcgagca ccgtgcccac cgtccccgtc
     8041 gggtcctcga gctcggcgta ggcgcgacct tcgatcgtcg ccgtgccggc cgtcgggcgg
     8101 accagcccca gcagcgcgcg cagcgtcgtc gtctttcccg caccgttcgg tccgaggaag
     8161 ccgacgatcc tgccacgctc gaccgagaac gagagatcgt cgaccgcgag cgtggcgccg
     8221 aagcgcttgc tcaggcccct gacctcgata gcggcttcgg tagcggtcat gccgtcgcat
     8281 ccttgcagga gcggcggcgc cacccgcgcg ccggtacgct gccgtccggc atggcggcgg
     8341 agagctacga cccacgcgag atcgagcgcc gctggcagcg cgtctgggca gacgagcgct
     8401 cgtgggaggt ctccaacgag gacgacggac ggccgaagtc ctacgtcctc gagatgctgc
     8461 cctacccgtc cggcgagccc cacatcggcc acctcaaggt ctactcggtc ggcgacgcga
     8521 tcgcccactt caagcgccga accggccacc gcgtgctgca cccgctcggc tacgacgcct
     8581 tcggcctgcc ggccgagaac cacgcgatca agaccggcca gcacccgcgc gagtcgaccg
     8641 aggcgtcgat cgccgagttc cgccgccagt tcaacgactg gggcgtctcg atcgactggt
     8701 cgcgcgagct tgggacgcac gagccgtcct actaccgctg gacgcagtgg ctgttcctgc
     8761 ggctgttcga gcagggcctg gcctaccgca agcaggccgc tgtgaactgg tgccccaacg
     8821 atgcgaccgt gctcgccaac gagcaggtcg tcgacggtcg ctgcgagcgc tgtggcgcgc
     8881 tcgtcgagca gcgagagctc gagcagtggt tcttcaggat caccgactac gccgaccgcc
     8941 tgctcgaaga cctcgagacg atcgactggc cgcagaacgt cgtcacgatg cagcgcaact
     9001 ggatcggccg ctccgagggc gcggaggtcg tcttccattg cgacgagctc gacatcgact
     9061 acccggtctt caccacgcgg ccggacacgc tcttcggcgc gacgttcttc gtgatggcgc
     9121 ccgagcaccc cgacgtcctg cggctcaacg actcgccgga ggttcgcgac tacgtccggc
     9181 gcgcgctgag cgcgtcggcc gaggagcgcg gcgaggagga ccgtgagaag accggcgtgg
     9241 cgctcgggcg cagcgtcacg aaccccgtca acggcgagca gataccgatg tacgtcgccg
     9301 actacgtgct gatggagtac ggcaccggcg cgatcatggc cgtgccggcc cacgacgagc
     9361 gcgaccacga gttcgcggtc aagcacggga tcgaggtccg gcgcgtgatc gagggcggcg
     9421 aggacgacga gctgccctac accggcgacg ggccgatggt cgccagcggg cgcttcgacg
     9481 ggctgcacaa ccgcgaggcg ttcgagcgga tcgtcgcctg gctcggagac gagggcaagg
     9541 gcgagccggc cgtcaactac cgcctgcgcg actggctgct gtcgcgccag cgctactggg
     9601 gctgcccgat cccgatcgtc cattgcgacc gctgcggctt ggtgccggtg cccgacgcgc
     9661 agctaccggt cgtgctgccc gacatcgacg actacgcccc cgagggccgc tcgccgctcg
     9721 cggccgccga ggactgggta gccgtcgact gcccgaggtg cggcgacccg gcgcggcgcg
     9781 agaccgacac gatggacacc ttcgtcgact cgtcgtggta cttcctgcgc tactgcgacg
     9841 cgcgcaacga ctccgcgccc tgggaccgcg acgtcctcgc tggctggatg ccggtcgacc
     9901 agtacatcgg gggcgtcgag cacgcgatcc tgcacctgat gtacgcgcgt t
//
LOCUS       DATABV010000068         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2109361, whole genome shotgun sequence.
ACCESSION   DATABV010000068 DATABV010000000
VERSION     DATABV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109361"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1860
                     /locus_tag="VHF88_02745"
     CDS             115..1860
                     /locus_tag="VHF88_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160715.1"
                     /translation="MPRSVSSVAAPPGGRPPEAAPPTATAPGAAGDRPSLRQRIDWWR
                     IAPTLISAALAAVYLYFKPRSPDLAAHIFRAELFGREGFTVWNGQWYGGHHTPAYSVL
                     SPPLSWLLGPQLMGALSAIAATICFTELMRKAYGAVAARLGTIWFGVGSATLLFTNRL
                     PFALGVAFGMAAVLAAQRRRRVLAPLLAVLSAISSPVAGLFLAMGGVGHALANWRGAK
                     RQAGDTDAAAVRTRRARARRDGIVLAAAAFLPPVILTITFPEGGWAPFPFSSYAPIPL
                     FALVCILLLPRAERTLRWTAALYGIGATLALVLHTAMGGNAVRIGALVGGPLLACALW
                     PRLGRRPMIPMIALLGALAFWQWSPAVRDIYKAIDDPVAKASYFDPLREYLALEPGQW
                     RVEIPFTFGHWEGAEVASEAPLARGWLRQLDTGRHPIFYGGQINELTYASWLSENAVR
                     FVALPDAKPDASSYRERALIESGLPYLRLRKRFDHWRIYEVTLPAPFVIPEGDANIQL
                     EQLGSDRVLLRVRRPGSAVVRVRWTPYWLAKGGCVERAGEWTRVTAEKPGFLPLVTRF
                     SPERIFQRGQRCNSS"
BASE COUNT          227 a          800 c          693 g          308 t
ORIGIN      
        1 ggtgatccgc agccgcgggc acgtgctggt gacgcggcgg atgctcgccg ccgcgaagcg
       61 cctcgccgag cgcaagcccg cgtaggggcc gctgcgttca tctacccttg gcgcgtgccg
      121 cgctcggtct cctccgtcgc ggcgccgccg gggggccggc cgccggaggc agcgccgccg
      181 acagccaccg ccccaggcgc cgcgggggac cggccgagcc ttcggcagcg gatcgactgg
      241 tggcggatcg cgccgacgtt gatctccgca gcgctcgccg ccgtctacct ctacttcaag
      301 ccgcgctccc cggacctcgc cgcgcacatc ttccgcgcgg agctcttcgg ccgcgagggg
      361 ttcacggtct ggaacggcca gtggtacggc ggccaccaca cgcccgccta ctcggtcctc
      421 tcgccgccgc tcagctggct gctcggaccg cagctgatgg gcgcgctgtc ggccatcgcg
      481 gcgacgatct gcttcaccga gctgatgcgc aaggcctacg gggccgtcgc cgcgcggctg
      541 gggacgatct ggttcggcgt gggctcggcg acgctgctgt tcaccaaccg cctgccgttc
      601 gcgctcggcg tcgcgttcgg gatggcggcc gtgctggcgg cccaacgtcg ccgtcgcgtc
      661 ctcgctcctc tactcgccgt gctcagcgcg atctcgagcc cggtcgccgg gctcttcctc
      721 gcgatgggcg gcgtcggcca tgcgctcgcc aactggcgcg gcgcgaagcg ccaggccggc
      781 gacaccgacg ccgcagcggt ccgcacccgc cgtgcccgcg cccgccgcga cggcatcgtc
      841 ctcgcggccg cggcgttcct cccacccgtc atcctcacga tcaccttccc cgagggcggc
      901 tgggcgccgt tcccgttctc ctcctacgcg ccgatccccc tgttcgcgct cgtctgcatc
      961 ctgctcctcc cgcgcgccga gaggaccctg cgctggaccg ccgccctcta cggcatcggc
     1021 gccacgctcg cgctcgtcct gcacaccgcg atgggcggca acgcggtccg gatcggcgcg
     1081 ctcgtcggcg gcccgctgct cgcctgcgcg ctctggccgc ggctcggccg ccgaccgatg
     1141 atcccgatga tcgcgctgct cggagcgctc gcgttctggc agtggtcgcc cgcggtgcgc
     1201 gacatctaca aggcgatcga cgacccggtc gccaaggcgt cctacttcga cccgctgcgc
     1261 gagtacctcg cgctcgagcc gggccagtgg cgagtcgaga tcccgttcac gttcggccac
     1321 tgggagggcg ccgaggtcgc gtccgaggcg ccgctcgcgc gtgggtggct gcgtcagctc
     1381 gacaccggcc gccacccgat cttctacggc ggccagatca acgagctgac ctacgcgagc
     1441 tggctgtcgg agaacgccgt ccgcttcgtc gcgctgcccg acgccaagcc cgacgccagc
     1501 tcctaccgcg agcgcgcgct gatcgagagc ggcctcccct acctgcggct gcgcaagcgc
     1561 ttcgaccact ggcggatcta cgaggtgacg ctgccggccc cgttcgtgat ccccgagggc
     1621 gacgcgaaca tccagctcga gcagctcggc tccgaccgcg tgctgctgcg cgtgcgacgg
     1681 ccgggcagcg ccgtggtgcg cgtgcgctgg accccctact ggctcgcgaa gggcggctgc
     1741 gtcgagcgcg ccggcgagtg gacgcgcgtg accgccgaga agcccggctt cctgccgctc
     1801 gtcacccgct tctcgcccga gcggatcttc cagcgcggcc agcgctgcaa cagcagctag
     1861 gcgcggtacc ctgccctgat cgtgcgccag gtctacgacg acttcacgct gcagttccgg
     1921 cgcgcctggc gggtgagcag ccgctggctg ccccagggct ggatcgacgc gctgcgccag
     1981 ctcgcgctgt tcgcgggggc ctattacgtc taccgaatcg tccgcggc
//
LOCUS       DATABV010000069         7070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1548875, whole genome shotgun sequence.
ACCESSION   DATABV010000069 DATABV010000000
VERSION     DATABV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548875"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VHF88_02750"
     CDS             <1..405
                     /locus_tag="VHF88_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX2160716.1"
                     /translation="VGAPARLDRQHDALGAAASGYEVVVHGGRTETGREAVEWAREGV
                     ERGAGEILLTSMDRDGTEDGYELELTRAVADAVDIPVIASGGAGKLDHLVDAVEQGRA
                     DAVLCASIFHYGQYTVREAKERMRIAGIPVRL"
     gene            532..1779
                     /locus_tag="VHF88_02755"
     CDS             532..1779
                     /locus_tag="VHF88_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160717.1"
                     /translation="MDRVLPAIEGAAPCYLVGGAVRDLLLGREPLDIDVAVEGDVEDV
                     ADRLAAALGGEVTAHERFGTATVSAGGLDVINLARTRREMYPAPGALPEVEPATLDED
                     LGRRDFTINAMALGLNRDRAGELRDPHDGRSDLRDGLIRILHPASFSDDPTRLLRAAR
                     YAARLGFALERDTERWARAAIADGALATVSGSRIRDELIDLLAEDEAPRATELLDELG
                     IDSALHRGLQANPELVASAKLGAAETGADPALTALAALGTLGTAHCDEAVPIGDLQAA
                     ETSVSPGEGLAAWTDRLGLAASARDAVVRAARRAPRLVTELRRDLRPSELHELLDGEP
                     PEALALALGLGAPAEPVLDFVSRLSHTRLEITGADLLAAGLPESPALGRALDETFARK
                     LDGEVDGREEELRVACEIARGER"
     gene            1776..2525
                     /locus_tag="VHF88_02760"
     CDS             1776..2525
                     /locus_tag="VHF88_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX2160718.1"
                     /translation="MSSRIQAHEATGSPPSIRVELPGAEVRFSTRLGGVSEAPYDSLN
                     LGIQTADVVERVLENRQLLAAAVGLGPAQIALGHQVHGVEIARWDAAPGVEFTDPVAP
                     RPDVDAHLTAQRGLGLLVQVADCLPVALASREQVAMVHCGWRGLAGGILARAVAAFDD
                     EPAAAIGPGIGRCCFKVGSEVLAEFADVDGAADGRMLDLRLVAAARLRAAGVEHVEHV
                     DLCTSCRQDLFFSHRRDAGVTGRQCGIVWRT"
     gene            2522..3166
                     /locus_tag="VHF88_02765"
     CDS             2522..3166
                     /locus_tag="VHF88_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HEX2160719.1"
                     /translation="MTRQFTDLDSARVAANLDRVRERIAAAGRDPGEVELLAAIKYVP
                     LGELPKLAEAGVELVGENRAQDLVAKQRDYGELFEWDFIGALQSRKVKDIAPVTRLIH
                     SVASESALAQLARHPAREVLVQVNVAGEDGKAGIAPSDLAAFLERCPVPVGGLMTMPP
                     FVERAEDNRRHFARLAELAAEHGLARLSMGTSQDYEIAVQEGATIVRLGSTLYG"
     gene            complement(3215..4090)
                     /locus_tag="VHF88_02770"
     CDS             complement(3215..4090)
                     /locus_tag="VHF88_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2160720.1"
                     /translation="MPAEPRREPVPFGVERRDGVTLAGEAAGDGEPVVLVHGLTATRR
                     NVVQGSRHLLGRGRRLVSYDARGHGESSPSPDPDAYEYVDLAGDLEAVLDAHDLERVP
                     IAGSSMGAGTAVRLALDRPGRVSALVLITPAYREPGQLGADDLAHWDRLAAALETGDV
                     EAFVDASGANELPARWREPARLATRQRIERHRDLGAVARALRVVPRSQPFAGLDELER
                     IDIPVLVVGTRDEADPGHPLATAEEYARRLPHAELAVEDPGDTPLAWQGARLSRAIGD
                     FLESHSCGTRRTRSG"
     gene            4173..4712
                     /gene="sepF"
                     /locus_tag="VHF88_02775"
     CDS             4173..4712
                     /gene="sepF"
                     /locus_tag="VHF88_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX2160721.1"
                     /translation="MALSDTWHRTLVYFGLAEEDDPRYEVVQDHEPEAELEDRYRERP
                     NVRRLQRKRRDDFDDIFADDSPRDSGRETRVLRSVGGNGARELDLQVHLVVPKNFNDA
                     QQIADRYKDDVPVILNLQQSDPDLSKRLIDFSSGLTYALDGGMQRIADKVFLLTPRNV
                     DVSAEERARLIEKGFFNQS"
     gene            4742..5500
                     /gene="proC"
                     /locus_tag="VHF88_02780"
     CDS             4742..5500
                     /gene="proC"
                     /locus_tag="VHF88_02780"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX2160722.1"
                     /translation="MKIGFLGAGSMATALARGLGEPALAFDVQEDRAAALAESVKGEA
                     VGSAHELAERADVVVLAHKPAHLEDASEGVAGTAKAVASIVAATPISRLEQVYPDVPV
                     YRFIPNIPVEVGRGVFCYVAGTHAERGPQQELLQLFGRAGAVIELDEPLLEPATALMS
                     CGPAFLALIVEALADAGSRHGLDPDQARRLVVETMAGSAAYLDANGLDAEELRGRVAT
                     PGGLTEKGLRLLEERGLRDALDGAVDLVVEASRR"
     gene            5497..5799
                     /locus_tag="VHF88_02785"
     CDS             5497..5799
                     /locus_tag="VHF88_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX2160723.1"
                     /translation="MTLIALTRDDVAAYVETLALVYLVLIFIRIVISWIPRMPYNRWL
                     NVFLTFVTDVTDPYLNLFRRFIPPLRVGPGALDLSPIIGTFVLLIVSGLVAGLIRG"
     gene            5802..6284
                     /gene="lspA"
                     /locus_tag="VHF88_02790"
     CDS             5802..6284
                     /gene="lspA"
                     /locus_tag="VHF88_02790"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX2160724.1"
                     /translation="MAAGSRAAAGWGRLLAVVAVVIALDQLTKRLVVASVERGDSVNV
                     FLGLDITNTRNSGVAFGALQDSGTIVALLVGCALVALVGYFAVNAGRPWLWAPAGLLL
                     GGALGNLIDRIAEGAVIDFIDPIAWPAFNVADACIVVGVLGLLYVVELGPGERRPAAE
                     "
     gene            6281..6994
                     /locus_tag="VHF88_02795"
     CDS             6281..6994
                     /locus_tag="VHF88_02795"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX2160725.1"
                     /translation="MTAAPRIAYEDEHLLVIDKPAGVVVHPGAGVSDGTLVQQLAGRV
                     AGGPDPDRPGVVHRLDRDTSGLLVLARSDEVHTALQAAIRDRAVEREYLALVSGRPGS
                     RTGTIDAPIGRDRANRTLMSTRTDRPRHAVTHFEVLEELPRHALLRVTLETGRTHQIR
                     AHMAAIGHPVCGDPAYGGRACGRELGLTRQFLHSARLVFSHPVTRERVECESKPPADL
                     RSALDAVRRGSASGGPAGS"
BASE COUNT          958 a         2584 c         2535 g          993 t
ORIGIN      
        1 gtcggcgcgc cggcgcgcct cgatcgccag cacgacgcac tgggcgccgc cgcctccggc
       61 tacgaggtcg tcgtccacgg cggtcgcacc gagaccggcc gcgaggcggt cgagtgggcc
      121 cgcgagggtg tggagcgcgg cgcgggggag atcctcctga ccagcatgga ccgcgacggc
      181 accgaggacg gctacgagct cgagctgact cgcgcggtcg ccgacgccgt cgacatcccg
      241 gtgatcgcct ccggcggcgc cggcaagctc gatcacctcg tcgacgccgt cgagcagggc
      301 cgcgctgacg ccgtcctctg cgcctcgatc ttccactacg gccagtacac ggtccgcgag
      361 gccaaggagc gcatgcgcat cgccggcatc cccgtccgtc tctgaccgat gaggggttgt
      421 gcagcccagg cgatgccaat ccctcattga ggtggcggtg ggggtcgctg cgacaatgcg
      481 ggcgtgctgg acgagctcga tctgcccgcg cgaatgcgga ccttgcgcgg aatggaccgc
      541 gtcctcccgg caatcgaagg cgccgcgccg tgctacctgg tcggaggggc ggtacgcgac
      601 ctgctgctgg gcagggagcc gctggacatc gacgttgcgg tcgaagggga cgtcgaggac
      661 gtggctgacc gcctggcggc agccttgggc ggcgaggtga ccgcccatga gcggttcggg
      721 acggccaccg tctcggcggg cggcctcgac gtgatcaacc tggcgcggac gcgtcgcgag
      781 atgtacccag cgcccggcgc gctgcccgag gtggagccgg ccaccctgga cgaggatctc
      841 ggccgccgcg acttcacgat caacgcgatg gctctcggcc tcaaccgtga ccgagccggt
      901 gagctgcgcg atccgcacga cgggcgaagc gacctacgcg acggtttgat tcgcatcctc
      961 cacccagcca gtttcagcga cgacccgacc cgcctgctgc gcgcagcgcg atatgcagcc
     1021 cggctcggct tcgcgctgga gcgcgacacg gagcgctggg cccgcgcggc gatcgccgac
     1081 ggtgcgctcg cgacggtctc cggctcgcgc atccgcgacg agctgatcga tctcctggcc
     1141 gaggatgagg cgccccgtgc cacggagctg ctggacgaac tgggaatcga ctccgcgctc
     1201 catcgaggcc tgcaagcgaa ccctgagctc gtggcgtcgg cgaagctggg cgccgccgag
     1261 accggcgccg acccggcact gaccgccttg gcggccttgg ggacgctcgg cacagcccac
     1321 tgcgacgagg cggtaccgat cggcgatctc caagccgccg agacgagcgt gagccctggc
     1381 gaaggcctcg cggcctggac cgatcggctc gggttggccg ccagcgcgcg cgatgccgtc
     1441 gttcgcgccg cccggcgtgc gcctcgcctg gtcaccgagc tgcggcgcga cctgaggccc
     1501 tccgagctgc acgagctgct cgacggcgag ccccccgagg cgctcgcgct cgcgctcggc
     1561 ctcggcgcgc ccgccgagcc ggtgctcgac ttcgtgtcgc ggctgagtca cacgcgcctc
     1621 gagatcaccg gcgcggacct gcttgcggcg ggacttccgg agtcgccggc tctcggccgc
     1681 gcgctcgacg agacgttcgc gcgcaagctc gacggtgaag tcgatggccg cgaggaagag
     1741 ctgcgcgtgg cctgtgagat cgcgaggggt gagcgatgag cagccggatc caggcccacg
     1801 aggcgaccgg ctcgccgccg tcgatcaggg tcgagctccc aggcgccgag gtgcgcttct
     1861 cgactcgtct cggcggtgtc agcgaagccc cgtacgactc gctcaacctc ggcatccaga
     1921 ccgccgacgt cgtggaacgg gtgctcgaga accggcaact gctcgccgcc gccgtcggct
     1981 tgggccccgc gcagatagcg ctcggccacc aggtccacgg cgtcgagata gcgcgctggg
     2041 acgcggcgcc tggcgtcgag ttcaccgacc cggtagcccc gcgccccgac gtcgacgctc
     2101 acctcaccgc gcagcgcggc ctcggcctgc tcgtccaggt ggccgactgc ctgccggtcg
     2161 cgctcgcgtc gcgggagcag gtcgcgatgg tgcactgcgg ctggcgcggg ctcgccggcg
     2221 gcatcctcgc gcgcgccgtg gccgcattcg acgacgagcc ggcggcggcg atcggtcccg
     2281 gcatcggccg ttgctgcttc aaggtggggt ccgaggtgct cgccgagttc gccgacgtcg
     2341 acggcgccgc cgacgggcgg atgctcgacc tccgcctggt cgccgcggcg cgcctgcgcg
     2401 ccgcgggcgt cgagcacgtc gagcacgtcg acctctgcac gtcctgccgc caggacctgt
     2461 tcttctccca ccgccgcgac gccggcgtca ccggccgcca gtgcggcatc gtctggcgca
     2521 cgtgacacgt cagttcaccg atctcgactc ggcgcgcgtg gccgccaacc tcgaccgcgt
     2581 gcgcgagcgg atcgccgcgg ccggtcgcga ccccggcgag gtcgagctgc tcgccgcgat
     2641 caagtacgtg ccgctcggcg agctgccgaa gctcgccgag gccggggtgg agctcgttgg
     2701 cgagaaccgc gcccaggacc tcgtcgccaa gcagcgcgac tacggcgagc tgttcgagtg
     2761 ggacttcatc ggcgcgctcc agagccgcaa ggtaaaggac atcgcgccgg tcacgcggct
     2821 gatccactcg gtcgcctcgg aatccgcgct cgcgcagctc gccaggcatc cggcgcgcga
     2881 ggtgctggtc caggtcaacg tggcggggga ggacggcaag gcaggcatcg cgccgagcga
     2941 cctcgccgcc ttcctcgagc ggtgcccggt tcccgtcggc ggcttgatga cgatgccgcc
     3001 cttcgtcgag cgcgccgagg acaaccgccg ccacttcgct cgactcgccg agctcgccgc
     3061 agagcacggc ctcgcccgcc tgtcgatggg cacgtcgcag gactacgaaa tcgccgtcca
     3121 ggagggcgcc acgatcgtcc gcctgggatc caccctttat gggtgagcgc gggcggaggg
     3181 cgcagccgga cgggtcccac aagtcgtacc gcagctagcc ggagcgagtc cggcgagtac
     3241 cgcagctatg actctccagg aagtcgccga tcgcgcgcga gagccgcgcg ccctgccagg
     3301 ccagtggcgt gtcgccgggg tcctcgacgg cgagctcggc gtgcggcagc cgccgcgcgt
     3361 actcctcggc cgtcgcgagc gggtggccgg gatcggcctc gtcgcgtgtc ccgacgacca
     3421 gcaccgggat gtcgatgcgc tcgagctcgt ccaacccggc gaagggctgc gagcgcggca
     3481 cgacgcgcag cgcgcgcgcc acagcgccca ggtcgcgatg gcgctcgatg cgctggcgcg
     3541 tagccagccg cgccggctcg cgccagcgcg cgggcagctc gttcgctccc gaggcgtcga
     3601 cgaacgcctc gacgtcgccg gtctccagcg cggcggcgag ccgatcccag tgcgccaggt
     3661 cgtcggcccc gagctgcccg ggctcgcgat aggccggggt aatcaggacg agcgccgaga
     3721 cccggcccgg ccggtccagc gccagccgaa ccgcggtccc ggcgcccatc gagctgcccg
     3781 cgatcggcac ccgctcgagg tcgtgggcgt ccagcaccgc ctccaggtcg ccggccagat
     3841 ccacgtactc gtacgcatcc gggtccgggg aggggctcga ctcgccgtgg ccccgggcgt
     3901 cgtaggagac cagccgccga ccgcgaccga gcaggtggcg cgatccctgg acgacgttgc
     3961 ggcgcgtcgc ggtcaggccg tggacgagga cgaccggctc gccgtcgccg gccgcctcgc
     4021 cggcgagcgt gacgccgtcg cggcgctcga cgccgaaggg gaccggttct ctacggggct
     4081 ctgcgggcat ctgaaggcag gttcctactc gataacgtgt aaacgcacgg ccgcaagggt
     4141 tttcggccga ctgctcgaaa agacaccgag cgatggctct cagcgacaca tggcaccgca
     4201 cgctcgtcta cttcggcctc gccgaggagg acgacccccg ctacgaggtc gtgcaggacc
     4261 acgagcccga ggccgagctc gaggaccgct accgcgagcg gccgaacgtc cgcaggctcc
     4321 agcgcaagcg ccgcgacgac ttcgacgaca tcttcgctga cgactcgccg cgcgactccg
     4381 ggcgcgagac gcgcgtcctg cgctcggtcg gcggcaatgg ggcacgcgag ctcgacctcc
     4441 aggtccacct ggtcgtcccg aagaacttca acgacgccca gcagatcgcc gaccgctaca
     4501 aggacgacgt cccggtcatc ctcaacctcc agcagagcga cccggacctc tcgaagcggc
     4561 tgatcgactt ctcctcaggc ctcacctacg cgctcgacgg cggtatgcag cggatcgccg
     4621 acaaggtctt cctgctcacg ccgcgcaacg tcgacgtctc cgccgaggag cgggcccgct
     4681 tgatcgagaa gggcttcttc aaccagtcct gaccccttgg cgggctagag ttgcccgcct
     4741 catgaagatc ggcttcctag gcgcgggcag catggcgacg gcgctggcgc gcgggctcgg
     4801 cgagccggcg cttgccttcg acgtgcagga ggatcgcgcc gccgcgctgg ccgagtcggt
     4861 gaagggcgag gcggtcggct cggcccacga gctcgcggag cgtgcggacg tcgtcgtcct
     4921 ggcgcacaag ccggcgcacc tcgaggacgc ctcggagggc gtcgccggca cggcgaaggc
     4981 ggtcgcgtcg atcgtcgccg ccacgccgat cagccggctc gagcaggtgt accccgacgt
     5041 cccggtctac cgcttcatcc cgaacatccc ggtcgaggtc gggcgcggcg tcttctgcta
     5101 cgtcgcgggc acgcacgccg agcggggccc gcagcaggag ctgctgcagc tcttcggccg
     5161 cgccggtgcc gtgatcgagc tcgacgagcc gctgctcgag ccggccacgg cgctgatgag
     5221 ctgcgggccg gcgttcctcg cgctgatcgt cgaggcactc gccgacgccg gctcgcgcca
     5281 cgggctcgac cccgaccagg cccggcgcct tgtggtcgag acgatggccg gcagcgccgc
     5341 ctacctcgac gccaacgggc tcgacgcgga ggagctgcgc ggtcgcgtcg ccacgcccgg
     5401 cggcctgacc gagaaggggc tgcgtctgct cgaggagcgc ggcctgcgcg acgccctcga
     5461 cggcgcggtc gacctcgtcg tggaggcgag ccggcggtga cgctgatcgc cctcacgcgc
     5521 gacgacgtcg ccgcctacgt cgagacgctc gcgctcgtct acctggtgct gatcttcatc
     5581 cggatcgtca tctcgtggat cccgcggatg ccctacaacc gctggctgaa cgtcttcctg
     5641 accttcgtca ccgacgtcac ggatccgtac ctcaacctct ttcgccgctt catcccgccg
     5701 ctgcgcgtgg ggcccggtgc gctcgacctc agtccgatca tcggcacgtt cgtgctgctg
     5761 atcgtcagcg gcctggtcgc cggcctgatc aggggctgag cttggctgcc ggctcgcgcg
     5821 ccgccgccgg ctggggacgg ctgcttgcgg tggtggcggt cgtgatcgca ctcgaccagc
     5881 tgacgaagcg cctcgtcgtc gcgagcgtcg agcgcggcga ctccgtcaac gtctttctcg
     5941 gcctcgacat cacgaacacc cgcaacagcg gcgtcgcgtt cggcgctctc caggacagcg
     6001 gcacgatcgt cgcgctgctc gtcggctgcg cgctggtggc gctcgtcggc tatttcgccg
     6061 tcaacgccgg ccggccgtgg ctgtgggcgc cggcgggcct gctgctcggc ggggcactcg
     6121 gcaacctgat cgacaggatc gccgaggggg cggtgatcga cttcatcgac ccgatcgcct
     6181 ggcccgcgtt caacgtcgca gacgcctgca tcgtggtcgg cgtgctgggc ctgctctatg
     6241 tcgtcgagtt agggccgggc gaacggcggc ccgcggcgga atgaccgccg cgccacgcat
     6301 cgcctacgag gacgagcacc tgctggtcat cgacaagccg gcgggggtcg tggtgcatcc
     6361 gggtgccggc gtcagcgacg gcacgctggt ccagcagctc gccggccggg tcgccggcgg
     6421 accggacccc gaccgcccgg gcgtggtcca tcgcctcgac cgcgacacct ccggcctgct
     6481 cgtcctcgct cgctcggacg aggttcacac cgctcttcaa gccgcgatcc gcgaccgggc
     6541 ggtggagcgc gagtacctcg cactggtgtc ggggcgtccg gggtcgcgga ccgggacgat
     6601 cgacgcaccg atcggtcgcg accgcgccaa ccggacgctg atgtccacgc gcaccgacag
     6661 gccgcgccac gcggtgaccc acttcgaggt gctcgaggag ctgccgcgcc acgcgctgct
     6721 gcgggtgacg ctcgagaccg ggcgcacgca tcagattcgc gctcacatgg cggcgatcgg
     6781 ccacccggtc tgcggtgatc cggcctacgg cggacgcgcc tgcggtcgcg agctggggct
     6841 gacgcgtcag ttcctgcaca gcgcgcggct ggtgtttagc catcccgtga cgcgggaacg
     6901 ggtcgagtgc gagtccaaac cacccgccga cctgcgtagt gcgcttgatg cggttaggcg
     6961 gggctcagct tccggagggc cagctgggag ctgacatcgg ggcgggacgg cctgagggtc
     7021 gtcccgcttc gctttggtga gtgaggtcgg ggggctcgcg ccccccgggc
//
LOCUS       DATABV010000070         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_263080, whole genome shotgun sequence.
ACCESSION   DATABV010000070 DATABV010000000
VERSION     DATABV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263080"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VHF88_02800"
     CDS             complement(<1..726)
                     /locus_tag="VHF88_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2160726.1"
                     /translation="MTWNPAESTVLVTGAQGFIGGWLAERLLDDGARVVVPRRDFEPD
                     ARFRTDGIEERCVVVAADVQDYAAMTRVLEEHHVDAVFHLAAQTIVGTANRSPLSTFE
                     ANVRGTYVLLEACRAAAATGGPVARIVVASSDKAYGSHDELPYREDFALQPTFPYDVS
                     KACTDMIARSFASTYGMPVAVTRLANVYGGGDLNWSRIVPDSARALADGDRPVIRSDG
                     SPERDFLYVEDAVDAYLAVAESLD"
     gene            complement(743..1018)
                     /locus_tag="VHF88_02805"
     CDS             complement(743..1018)
                     /locus_tag="VHF88_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX2160727.1"
                     /translation="MSVTVKIPTQLRSVTGGEAETAVDEATTVGEVLDGLYDRFDGLR
                     DRIAEDGGLRRFVNVYVGGEDIRFLDGLDTAVDDGDEVTILPAVAGG"
     gene            complement(1042..2271)
                     /gene="thrC"
                     /locus_tag="VHF88_02810"
     CDS             complement(1042..2271)
                     /gene="thrC"
                     /locus_tag="VHF88_02810"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2160728.1"
                     /translation="MALEALKCKECGTTYPLEARYVCDDCFGPLEVAYDFSDLDAAST
                     RRKIQAGPQSIWRYSDFLPFESQPRTALAAGVTPLLRADRLAERLGLGEVWVKNDAAN
                     PTHSFKDRVVTVALAKAQELGYEVVACASTGNLANAVAAHAAAAGLESYVFVPADLEE
                     QKILATGVYGTNLVAVNGNYDDVNRLCTELSGERDWAFVNVNMRPYYSEGSKTVGYEI
                     AEQLGWELPDRVVVPVASGSLFTKIARGFEEWREIGLVDGELPHFNGGQAEGCSPVAQ
                     AYEAGRDFCAPVKPETIAKSLAIGNPADGPYALDLARRTEGGIDAVTDDEIRAGIRLL
                     AETTGIFTETAGGVTTATLAKLAERGEIGRDERVVLVITGEGLKTLDAVRGSFETIDV
                     DPTAESFASAAGVEAIA"
     regulatory      complement(2339..2457)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2531..>3271
                     /locus_tag="VHF88_02815"
     CDS             2531..>3271
                     /locus_tag="VHF88_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX2160729.1"
                     /translation="MPSIPRPRLRRTPRIGPDSGPSAAASEPNVEIVEWSGAAAVDAP
                     ARPLRWINIERPGPVDRAWLQEHFDFHPLDYEDVASRNQRPKIDEYPDYLFIVLHFPV
                     YDKSVGRLNTAELDMFVGPDFLITLPNRPIPPVEYLFERCRSSDEMLEQLLSKGPGYL
                     LYKIVDDSFDYCFPMLRKMGNKLEQVEEDIFLGRSEELVRDISNVKQEIINFRKIIRP
                     QRSTLRDLERTKQRYMAADLDVYFDDIQD"
BASE COUNT          495 a         1147 c         1108 g          521 t
ORIGIN      
        1 atcgagcgac tcggccacgg cgaggtaggc gtcgacggcg tcctcgacgt agaggaagtc
       61 ccgctcgggc gagccgtccg agcggatcac cggccggtcg ccgtcggcca gcgcccgtgc
      121 gctgtcgggc acgatgcgcg accagttgag gtctccgccg ccgtagacgt tcgcgagccg
      181 cgtgaccgcc actggcatcc cgtacgtcga cgcgaagctg cgcgcgatca tgtcggtgca
      241 ggccttcgag acgtcgtagg ggaacgtcgg ctggagggcg aagtcctcgc gatagggcag
      301 ctcgtcgtgt gagccgtagg ccttgtccga cgatgcgacg acgatccgag cgaccggacc
      361 gcctgtcgcg gccgcggcgc ggcaggcctc gagcagcacg taggtcccgc gcacgttggc
      421 ctcgaacgtc gacagcggcg agcggttcgc ggtgccgacg atcgtctggg cggcgaggtg
      481 gaagacggcg tcgacgtggt gctcctcgag cacgcgcgtc atcgccgcgt agtcctggac
      541 gtcggcggcg acgaccacgc agcgctcttc gatgccgtcc gtgcgaaagc gcgcgtcggg
      601 ctcgaagtcg cgccgcggga cgacgacacg tgcgccgtcg tcaagcagcc gctcggcgag
      661 ccagccgccg atgaaccctt gagcgcccgt cacgaggacg gtcgactccg ccgggttcca
      721 ggtcactgcg gagccgcggc gactagccgc cggcgacggc cggcaggatg gtcacctcgt
      781 cgccgtcgtc gacggccgtg tcgaggccat ccaggaagcg gatgtcctcc ccgccgacgt
      841 agacgttgac gaagcggcgc agtccgccat cctcggcgat gcggtcgcgc aggccgtcga
      901 agcggtcgta gaggccgtcg aggacctcgc cgaccgtcgt cgcctcgtcg acggccgtct
      961 cggcctcgcc gccggtgacg ctgcgcagtt gcgttgggat cttgaccgtg acgctcatat
     1021 cgcgcggcga gtctacagcc gctaggcgat ggcttcgacg cccgccgcgc tggcgaacga
     1081 ctcggccgtc ggatcgacgt cgatcgtctc gaagctgccg cgcaccgcgt cgagggtctt
     1141 caggccctca ccggtgatca ccagcacgac gcgctcgtcg cgcccgatct cgccgcgctc
     1201 ggcgagcttc gcgagcgtcg cggtcgtgac gccgcccgca gtctcggtga agattccggt
     1261 cgtctcggcg agcagcctga ttcccgcgcg gatctcgtcg tcggtcacag cgtcgatgcc
     1321 gccctcggtg cggcgggcca ggtcgagcgc gtatgggccg tcggccgggt tgccgatcgc
     1381 cagcgacttg gcgatcgtct ccggcttcac cggcgcgcag aagtcgcgtc cggcctcgta
     1441 ggcctgggcg accggcgagc agccctccgc ctgaccccca ttgaaatgcg gcagctcccc
     1501 gtccacaagc ccaatctcgc gccactcttc gaagccgcgc gcgatcttcg tgaacagcga
     1561 gccggaggcg accggcacga cgacgcggtc cggcagctcc cagccgagct gctcggcgat
     1621 ctcgtagccg accgtcttcg aaccctcgga gtagtacggc ctcatgttga cgttcacgaa
     1681 cgcccagtcg cgctcgcccg agagctcggt gcagagccgg ttgacgtcgt cgtagttgcc
     1741 gttgacagct acgaggttgg tgccgtagac cccggtcgcg aggatcttct gctcctcgag
     1801 gtcggccggg acgaagacgt aggactcgag ccccgccgcc gcggcgtggg cggcgaccgc
     1861 gttggccagg ttgcccgtcg acgcgcatgc gacgacctcg tagccgagct cctgcgcctt
     1921 cgcgagcgcg accgtcacga cgcggtcctt gaacgagtgc gtcgggttgg cggcgtcgtt
     1981 cttgacccag acctcgccga ggccgagacg ctcggcgagc cggtcggcgc gcagcagcgg
     2041 cgtgacgccg gcggcgagcg ccgtgcgcgg ctgcgactcg aacggcagga agtccgagta
     2101 gcgccagatc gactgtggtc cggcctggat cttgcgccgc gtcgaggcgg cgtcgaggtc
     2161 ggagaagtcg taggccacct cgagcggccc gaagcagtcg tcgcagacgt aacgcgcctc
     2221 gagcggatag gtggttccgc actccttgca cttcagcgct tctagagcca tgccctgggt
     2281 cccttcatgt cgaaagcctc cgccgcgttg ctcgtgacgg aggcctctga agggcttcgc
     2341 cacatctttc agacccgtgg gcctgatgga cttggcacct ttcctcgcga ccggtggtcg
     2401 tctggatggt tgccggggtt tcgtagggcc atttccctcc acccctctgg atgcgtgcgg
     2461 ctatgtcagt gcaaggagga tagcgatcgc gaggacggat gcaaggctcg gggtaggctc
     2521 agtcggagcc atgccgagca ttcccaggcc gcgcctgcgg cgaacgccgc gcatcggacc
     2581 cgactccggg ccgtcggccg ctgcctccga gccgaacgtg gagatcgtcg agtggagcgg
     2641 cgccgcggcc gtcgacgccc cggcccggcc gctgcgctgg atcaacatcg aacgccccgg
     2701 cccggtcgac cgcgcctggc tgcaggagca cttcgacttc catccgctcg actacgagga
     2761 cgtcgcctcg cgcaaccagc gaccgaagat cgacgagtac cccgactacc tgttcatcgt
     2821 cctgcacttc ccggtctacg acaagtcggt cggccggctg aacaccgccg agctcgacat
     2881 gttcgtcggc cccgacttcc tgatcacgct gccgaaccgg ccgatcccgc cggtcgagta
     2941 cctcttcgag cgctgccgca gctccgacga gatgctcgag cagctgctct ccaagggccc
     3001 cggctacctg ctctacaaga tcgtcgacga ctcgttcgac tactgcttcc cgatgctgcg
     3061 caagatgggc aacaagctcg agcaggtcga ggaggacatc ttcctcgggc gctccgagga
     3121 gctcgtgcgc gacatctcca acgtcaagca ggagatcatc aacttccgca agatcatccg
     3181 gccgcagcgc tcgacgctgc gcgacctcga gcggaccaag cagcgctaca tggccgccga
     3241 cctcgacgtc tacttcgacg acatccagga c
//
LOCUS       DATABV010000071         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1549194, whole genome shotgun sequence.
ACCESSION   DATABV010000071 DATABV010000000
VERSION     DATABV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549194"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VHF88_02820"
     CDS             <1..638
                     /locus_tag="VHF88_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2160730.1"
                     /translation="EQAAAGAFANAGQICTSVERVYVHEQLAEPFLAALTDRARSIAV
                     GPGLDPRSEMGPLIDERQLELVQAHVAEAVAAGARVLAGGERLDRAGSFFAPTVLADV
                     DGAMRIMREETFGPVAPVQVVDSFDAALAEADATDYGLAATVLTPSQANAQRAWRELR
                     AGTVKVNAVWGGAPGGSAQPRGQSGNGFGYGPELLDELTLTKVVHIEPPPG"
     gene            complement(642..1634)
                     /gene="glpX"
                     /locus_tag="VHF88_02825"
     CDS             complement(642..1634)
                     /gene="glpX"
                     /locus_tag="VHF88_02825"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX2160731.1"
                     /translation="MNDAPKPDRNLALELVRTTEYAAIAASRWSGRGQKESADGAAVD
                     AMRLMLDTVGMDGTVVIGEGEKDEAPMLYNGERIGDGSPPHVDIAVDPLEGTELCAMG
                     LPSSLAVIALAERGTMFDPGPCVYMEKMATSKELAHLLDLDRPLGETLALIAKEKGTP
                     IGDVMVIMLDRPRHEDAVREIRDAGARIRFIPHGDVSASLTAVTQGSSVDLLWGIGGT
                     PEGVLSAAAIKCIGGKIVGRLWPRDDAERQAATDAGYDLSEVLDTDRLVAGDDVFFSA
                     TGVTDGDMLAGVRFTAEGATTESLVMRSRSGTVRRIHATHDRPKLRALMGSRVD"
     gene            1713..>2273
                     /locus_tag="VHF88_02830"
     CDS             1713..>2273
                     /locus_tag="VHF88_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2160732.1"
                     /translation="MEYAEALAEARERTLALVAPVAEHDLDRVHSPLMSPLAWDLGHI
                     AAFEDLWLCVRAGGLEPLRPELMAVYDASETPRAQRGEIPYLRRDDALEFMAQVRARA
                     LEVLEKADLSPGSDRLNANGFVWDLLIQHEHQHNETMLQTLALAEPGVFDRTAVEGSQ
                     LGSAPEAHGGPIPAPSPPRSAQVVADG"
BASE COUNT          308 a          812 c          844 g          309 t
ORIGIN      
        1 ccgagcaggc ggccgccgga gccttcgcca acgccggaca gatctgcacc tcggtcgagc
       61 gggtctacgt ccacgagcag ctcgccgagc cgttcctggc ggcgctgacc gaccgcgccc
      121 gctcgatcgc ggtcgggccc ggcctcgatc cgcgcagcga gatggggccg ctgatcgacg
      181 agcgtcagct cgagctggtc caggcgcacg tggccgaggc ggtcgcggcc ggcgcgcggg
      241 tcctcgccgg cggcgagcgg ctcgaccgcg ccggctcctt cttcgcgccg actgtcctcg
      301 ccgacgtcga cggcgcaatg cggatcatgc gcgaggagac cttcgggccg gtggcgccgg
      361 tgcaggtcgt cgactcgttc gacgcagcgc tcgccgaggc cgacgcgacc gactacggcc
      421 tcgccgcgac cgtgctgacc ccgtcgcagg ccaacgccca gcgcgcctgg cgcgagctgc
      481 gcgccggcac ggtcaaggtc aacgccgtct ggggcggcgc ccccggcggc agcgcgcagc
      541 cgcgcggcca gagcggcaac ggcttcggct acggcccgga gctgctcgat gagctgacgc
      601 tgacgaaggt cgtccacatc gagccgccgc ccggctgaag gtcagtcgac ccgcgagccc
      661 atcagtgcgc ggagcttcgg ccggtcatgg gtcgcgtgga tgcggcggac ggtgccggag
      721 cgggagcgca tgacgagcga ctccgtggtg gcgccctcgg cggtgaagcg gacgccggcg
      781 agcatgtcgc cgtcggtgac gccggtggcg gagaagaaga cgtcgtcgcc ggcgacgagg
      841 cggtcggtgt cgagcacctc cgagaggtcg tagccggcgt cggtggcggc ctggcgctcg
      901 gcgtcgtcgc gcggccagag acggccgacg atcttgccgc cgatgcactt gatcgctgcg
      961 gcggagagga cgccctcggg cgtgccgccg atcccccaga gcagatcgac cgacgagccc
     1021 tgcgtgacgg cggtcagcga ggccgagacg tcgccgtgcg ggatgaagcg gatgcgcgcg
     1081 ccggcgtcgc ggatctcgcg caccgcgtcc tcgtgacgcg ggcggtcgag catgatcacc
     1141 atcacgtcgc cgatcggggt gcccttctcc ttggcgatca gggcgagcgt ctcgccgagc
     1201 ggacggtcga ggtcgagcag gtgggcgagc tccttcgagg tcgccatctt ctccatgtag
     1261 acgcaaggcc cggggtcgaa catcgtcccg cgctcggcga gcgcgatcac cgccagcgag
     1321 ctcggcaggc ccatcgcgca gagctcggtc ccctcgagcg ggtcgacggc gatgtcgacg
     1381 tgcggcggcg agccgtcgcc gatccgctcg ccgttgtaga gcatcggcgc ctcgtccttc
     1441 tcgccctcgc cgatcacgac ggtgccgtcc atgccgacgg tgtcgagcat caggcgcatc
     1501 gcgtcgaccg cggcgccgtc ggcggactcc ttctgaccgc ggccggacca gcgcgaggcg
     1561 gcgatcgcgg cgtactcggt cgtccgcacc agctcgagcg cgaggttgcg gtccggcttc
     1621 ggggcgtcgt tcatcggcga gaccctaagc gaaagcggca gtccgaagcg gtgctcgccg
     1681 gccggcgatc ggcgctggtt acgcttccgg cggtggagta cgccgaggca ctcgcggagg
     1741 cacgcgagcg gacgctggcg ctggtcgcgc cggtggccga gcacgacctc gaccgtgtcc
     1801 actcgccgct gatgtccccg ctcgcctggg acctcgggca catcgcggcg ttcgaggacc
     1861 tctggctgtg cgtccgggcc ggcggcctcg agccgctgcg gccagagctg atggccgtct
     1921 acgacgcgag cgagacgccg cgcgcgcagc ggggcgaaat tccctacctg cgtcgtgacg
     1981 acgcgctcga gttcatggcc caggttcgcg cccgggcgct ggaggtgctc gagaaggcag
     2041 acctctcccc cggctcggac cggttgaacg cgaacggctt cgtctgggac ctgctgatcc
     2101 agcacgagca ccagcacaac gagacgatgc tgcagacgct ggcactcgcc gagccgggcg
     2161 tcttcgatcg caccgccgtc gagggaagcc agctcggatc agcccccgag gcccacggcg
     2221 ggccgattcc ggcgccatct ccgccgcgat cagcccaagt tgtcgcggac ggc
//
LOCUS       DATABV010000072        22468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_584686, whole genome shotgun sequence.
ACCESSION   DATABV010000072 DATABV010000000
VERSION     DATABV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22468
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584686"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VHF88_02835"
     CDS             <1..325
                     /locus_tag="VHF88_02835"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX2160733.1"
                     /translation="TSLERQVGDEGQTEFGELLPSDRPPPDEEVEIALTEQSLRRAVA
                     QLPDNERQVLRLRYGIDGAEPVALREAGRRLGVSSESVRNLEKAALRHLAENREVAAL
                     GQAAA"
     gene            355..1791
                     /locus_tag="VHF88_02840"
     CDS             355..1791
                     /locus_tag="VHF88_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160734.1"
                     /translation="MRATTAMGCLAAAALSIFVVAPAPAYDAWAWLLWGREVAGGALH
                     TAEGPAFKPLPVAVCALLSAFGDAAALLWVIVARTGALLAVVLAFRLGWRLMGEALGA
                     GGAGRSPLVGGLLAAAGVALCGSFLGYAAEGVVTGWLVALGLAGLEAWRAGRPRAALA
                     CAVACCLLHVEAWPFAAAFGLFAWLRRPSDRPLLAVAAIAVPALWIVPEWIGSGDPLR
                     SGARARIPNPGQPALADVPALASLEQSARLLLWPLWIGIAALALAAWRPSPGGGLPPR
                     AWSALGEGLGGAAALAAIGTAWVLLVALMAQFGGFSGEPRYATPGVALIAIAAAVGLA
                     SGIAAISRRAFGRAWIAAAVLGSALLVAGIARLDQLSAVPAAQAHQWRLANDLSEAVE
                     AAGGRDAVLSCGIPYVGPLRGPLLAHRLDVAKEVVEPDLPPRPPAVVFRSPRRAGARV
                     TPAARPVFRQIAVVGDWQVARACRVRKP"
     gene            1806..2954
                     /locus_tag="VHF88_02845"
     CDS             1806..2954
                     /locus_tag="VHF88_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2160735.1"
                     /translation="MPWRPPLITILLAVALAGCGGADANEEIPDPEREPATEAGKARH
                     FDVARIATGLNRPTFVGAAPGDTGALWVLEQPGRVVRLGPDGGRLVALDISDEVKVGG
                     EQGLLGIAFHPDFATDRRVYLHWSDPQGDTRVAELEARADGTIDGGARRALLRLDQPE
                     ENHNGGQLAFGPDGRLYLGLGDGGGSFDPNETAQDLDSPLGKVASLDVDAPRPRWRTE
                     LYGLRNPWRFSFDPALGELLVADVGQDETEEINRVLVEPDEPPKNLGWSAFEGTFRIH
                     ETHELDTRGELIWPVATYSHDDGCSVTGGFVYRGTALPRLSGRYVYGDFCSGSLWTLK
                     PTPDAFAEDVRREQAAVPQLTHIGEDSEGRIVFASATGALYRAAPPPR"
     gene            complement(3015..4214)
                     /locus_tag="VHF88_02850"
     CDS             complement(3015..4214)
                     /locus_tag="VHF88_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160736.1"
                     /translation="MRRSAMALGASLLALALHTAPAAAQTGDPGQQIGDSTGAAQVGA
                     VQADVPVRVLSDGDNAAPAGVTAAPQETGDSTATGQVGAVDVDAPVRVLSHGDNAAAD
                     GGGSTAAPQDTSDSAVTGQVGSVDADAPVRVLSDGDDTAAGDGSSTTAQDVDDSTGGA
                     QVGAVDADAPVRVLSDGDNASGSTQGTSSGTEQTARDSAGGAQVAPVRAAAPIRVLST
                     GDDDAGSDPGAGAGSQTVGDGGGGLAVGGAAAGAPLRIASRGNDGATASAVPDPVDDP
                     APGAGSRNGSPAPGDVLGAGTVPAVDRAPDRDPVAVPSPGAGPDGGSAPDGGSAPDGD
                     SLPDGGTLPDGASAPAGSTIPVAAGDVAGDMASGELPMTGLAIAVLALIGLSLLTGGG
                     ALRRAAG"
     gene            complement(4288..5124)
                     /locus_tag="VHF88_02855"
     CDS             complement(4288..5124)
                     /locus_tag="VHF88_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160737.1"
                     /translation="MTLVLVLGMAATPASAAAGPVEGLTQPVTKVAAPVTQAVDDQVQ
                     AAAPVVQQTVERTTAPVQATVDTVRAARPVRSTVEAVTQPVRETVERVAAVEPIRPAA
                     DAVRTPVAAAATGGAPAGVSTGVRHGGSAAQPRTGGAGHGDRADGTPRRDIGRIDREA
                     SPRSGDSATGGIAHDAVDALTASAGLGTLTLPAIAAGELARADASSTSAFGDDGDGDQ
                     VPFGPGGEGLLGGPGGIALIGLGLLVALLTLVPRFTSRLLQMSPTRWGAAAFRVRIER
                     PG"
     gene            complement(5291..6349)
                     /locus_tag="VHF88_02860"
     CDS             complement(5291..6349)
                     /locus_tag="VHF88_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2160738.1"
                     /translation="MDATAGGEPARRTIGVSQTARTVLTTAGIVGLIYVAWLLRDVLL
                     LAILAIFLAVALSAPVNLLQRRLGLPRALAILVVYVVLATVMVGIGLLVLPPLITEFT
                     AFLRAVPGYVRDVQDSGLIERWDREYGIVAHLEGEARNLPSILGGALSELETVTVAAI
                     ERVVELIAILAIAFLLLLDAPRLLGFVYREVAPDEAKARRVAAETSSAVGGYVAGVFA
                     VAALAGVVSFLVMTVLGIPYAVPLAVQMAFFAILPLVGSTIGAAVIAVVAAFEGPEIV
                     AIWAVFWLFYQQTETHVLGPLVYRKAVDMSPALVILSVMAGATLLGVLGALLAIPATA
                     TIQILIREWWRSRRLAVT"
     gene            6483..8687
                     /locus_tag="VHF88_02865"
     CDS             6483..8687
                     /locus_tag="VHF88_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2160739.1"
                     /translation="MSLLLARLARTITHHWKRSALAAVVAVVLLGAAAGAGGQAADDF
                     NVPGTESQQALDLFREHSPTFAGADSTVVFSVEQGTLDEPERRAAIEEAVAEVRALPG
                     VKEFGDPFAEGATMSEDGRIAAVDVRYDVEPDDIEREDGEALEEAARTAEAGGVDVSM
                     RGIVVDIASEQEAPVGELIGVAIAIVLLTLLFRSLAAMFATIVGALIGVMTGQMLLAA
                     LAKPLGLPEFAPVIAMMLGLGAGIDYALLIIGRYREQAAAGDSTRDAAAKAAGTSGAS
                     VVAAGLIVMVAIAGLLVVGIPFIGKLGVAAALAVGAVVVSALTILPIMVGALARWLKP
                     KKPEHVLPSRFFSRWGEIVSGRPWVAIGAAVALLLVLAFPVTQLRLGQPDDGNQPTEK
                     TQRIAYDLQSEAFGPGSNGPFLLAVDVPRHGADTNAQLGRIEAAVRDTPGVASVAPTQ
                     LSQDGEMATIFAIPTTAPQDAATSDLLERLREQTIPAATAGTPLKVYVGGNTATFEDL
                     SDKVASRLPLFIAVVIGLSVLLLIAAFRSIWVPLASAVMNLLSIGAAYGVVTAVFQEG
                     VGAGLLGVDSGVPIVSFIPVMMFAILFGLSMDYNVFLLSRIHEAYNEGDGPRASVIHG
                     MGRIGKVILFAGLIMAAVFLAFVSQPDIIGKMMGLGLGVAILIDVLVVRLVIAPAVVT
                     LLGDRAWWLPGWLDRILPNVSLEGHLVQSLDGKQADGDKATEEERPVEAPVA"
     gene            complement(8692..9330)
                     /locus_tag="VHF88_02870"
     CDS             complement(8692..9330)
                     /locus_tag="VHF88_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX2160740.1"
                     /translation="MARRSSFQDRRTAGQLLAARLVELDLDAPVVIALPRGGVPVAYE
                     VAQALGAPLDIALVRKLGAPGQPELGIGAVGEDGAVIVDRETVAALGVRRAQIDAAVE
                     REAVELARRRRLYRGNRPPIEVDGRTAILVDDGIATGVTAVAAARGLRDRGAARVITA
                     VPVCPASAVGRLRVELGELVCLEQPRRFGGVGAWYDDFTQTSDDEVMALLRR"
     gene            9394..9759
                     /locus_tag="VHF88_02875"
     CDS             9394..9759
                     /locus_tag="VHF88_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX2160741.1"
                     /translation="MRFMMFMLPGGELQETGDWGPPSADDVGEMTKYNEELSKAGVLL
                     ALDGLQPPSKGARVTLADGAKQVTDGPFAEAREIVGGYWIIDVKSKQEAVEWAKRCPL
                     EPGDVIEVRQVFEMSDFPE"
     gene            9756..11012
                     /locus_tag="VHF88_02880"
     CDS             9756..11012
                     /locus_tag="VHF88_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2160742.1"
                     /translation="MSHSDATHSIDAVWRIESPRLIAGLARMTGDVGVAEDLAQDTLV
                     AALERWPRSGVPDNPGAWLMATAKHRAIDLFRRNERLARKQEEIARDVGLQMAMDGAD
                     LDAALDDEIGDDLLALIFTCCHPVLSTDARVALTLRLLGGLTTDEIARAFLVAESTVA
                     QRIVRAKRTLGAAKVPFEVPRGDELAQRLPSVLEVVYLVFNEGYAATAGGDWTRPGLC
                     SDALRLGRILAGLTPKEPEVHGLLALMEIQASRLGARSGPGGVPVLLLDQDRSRWDPL
                     LIRRGLAALDRARQLGGGLGPYALQAAIAACHARARTPEETDWTTIAALYDALAQLAP
                     SPVVELNRAVAVGMAFGPEAGLALADQLADAPALERYHLLPAVRGDLLAKLGRHDEAR
                     VEFERAAALTENARERALLLERAASR"
     gene            11023..12021
                     /locus_tag="VHF88_02885"
     CDS             11023..12021
                     /locus_tag="VHF88_02885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2160743.1"
                     /translation="MSVPLSILDLAPVRSGESARDALAGVTEVARAADALGYRRFWVA
                     EHHNIPAVASTAPEVLIAHVAAKTERIRVGSGGVMLPNHAPLAVAERFGMLEALHPNR
                     IDLGLGRAPGTDMRTALALRSTSPESFADQLAQLRGHLGDGHAGIRAVSGEHTVPEIW
                     MLGSTDGGARIAAALGLPFVFAHHFHPHFTEPALRIYRDNFEPSDLLDRPRAIVAAAI
                     VVGEDDADASRLALPSAISMLRLRQGRPAPLPTVEEAEELLPRLGPNEWSVVQDTLSR
                     AIVGSSATVESRLLDLIERTATDELMLTSNVADPALRIQAFEQMAHAFALSPAAVG"
     gene            complement(12047..12553)
                     /locus_tag="VHF88_02890"
     CDS             complement(12047..12553)
                     /locus_tag="VHF88_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160744.1"
                     /translation="MTQPAVQTDMQLLGIYMNDQLAMGVIWREVAKRSAGSNEGTALG
                     EAVERVATQIAEDVETFERLMDRLGIERSRVKATLAIAGERVGRLKLNGRLFSYSPLS
                     RFVELDFLAAGIEGKRILWANLRDFAGLAERLPDVHFDHLIERAERQRAELEPFRAQA
                     GREAFLSK"
     gene            12612..13046
                     /locus_tag="VHF88_02895"
     CDS             12612..13046
                     /locus_tag="VHF88_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2160745.1"
                     /translation="MAFVPTARHLLRAKDLADARYFEPLDVDELAGAAGLSRAHFSRE
                     FRRAFGESPHAYLLTRRLERAADLLRNTDRSVADVCLSVGLQSIGSFTTSFKRTYGLS
                     PTAYRAKFPPAAQRALVPACVVRLRGRPQRRTFREDSPPGSD"
     gene            13086..13517
                     /locus_tag="VHF88_02900"
     CDS             13086..13517
                     /locus_tag="VHF88_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2160746.1"
                     /translation="MIRIANAQLWVHDQDEALAFYTEKVGMEVRADVTMPEMDGFRWL
                     TVGPAGQPDVSIVLMGVPGPPVFEPETKRQIEELVAKGVAGTVFLTTDDCQASYEELS
                     GRGVEFVDLPNEQPYGIDSSFRDPSGNHLRLIQLSEVPVGG"
     gene            complement(13558..13980)
                     /locus_tag="VHF88_02905"
     CDS             complement(13558..13980)
                     /locus_tag="VHF88_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2160747.1"
                     /translation="MTGRGPRAPAGAWTPEEAREFATRWLPAWSGNRPELLASFYTDD
                     AFYADPAVPDGVRGQEQLLGYFRRLLARHPDWEWRQHRATPLEDGFLNHWEADIPAGP
                     RTLTLRGVCTVQLRGDRIYRNEVFFDRSELLAAARQPR"
     gene            complement(13977..14789)
                     /locus_tag="VHF88_02910"
     CDS             complement(13977..14789)
                     /locus_tag="VHF88_02910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2160748.1"
                     /translation="MPFSRYVALGDSTTEGLDDPAPGGDGFVGFADRLAARLAREHPG
                     LLYANLAVRGRKAGQIRAEQLKPALAMEPDLATVVGGINDILRPRADLRYVLAEIEAV
                     ISALRDAGATVVGMTYPDPTHVMPVARPTRARVLAFNEGLREIAVRHGALLIDVERDG
                     IVDARLWSDDRLHASSVGHERLAHAAAQALGLEPDADPWAPLPPAESLRRAQAAAGHA
                     IWTARHMAPWILRRVRGRSSGDGMAAKRPELARIEAVVASTAAVGGTRGTEP"
     gene            14838..15221
                     /locus_tag="VHF88_02915"
     CDS             14838..15221
                     /locus_tag="VHF88_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2160749.1"
                     /translation="MAIATAPITGVDFVCIPTRDHDRAAEFYGGTLGLPKVKRWGDMP
                     ATEFQAGNLTLAVMDPSAFGMPEATPNSTPVAFQVDDVEATRKQLAAEGIEFQVELLD
                     SGVCHQAIFSDPDGNPLILHHRYAD"
     gene            complement(15241..16164)
                     /locus_tag="VHF88_02920"
     CDS             complement(15241..16164)
                     /locus_tag="VHF88_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2160750.1"
                     /translation="MSRFEQPDILVLGGGGILGEAWMSAVLAGLAEASDFDPHGCEAY
                     LGTSAGSIVAAALTAGVDPSSRLDELPEQPDVPDEAVGAEAPRPLARALGQAGKVAGP
                     LAALTLPFMAPGGALLRRKALSRVTPGRRSLAQLAAVVEDWGIDWDGRLRISAVDMDS
                     GRRVIFGEQGAPQATIAKAVQASCAIPGFFRPIEIEGRRYLDGGVWSPTNMDRAPAER
                     GSRVLCLNPTASLTPSREQPYGAMGLLSRSLAAIEAAYLERRGVRVQVVAPDPASTVA
                     MAGRLMDGRLRAATLAAGLAQGRALAASSSR"
     gene            16196..16960
                     /locus_tag="VHF88_02925"
     CDS             16196..16960
                     /locus_tag="VHF88_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2160751.1"
                     /translation="MSDPVYDRLGVGYSAVRATDPRIAAAIDEALGGARTVLNVGAGT
                     GSYEQLGRDVTAVEPSPEMIRQRPAGAAPVVQATAEDLPFDDDSFDAAMAIISDHHWS
                     DRAAGMAELRRVARERVVVLNADPSFAPRFWLSRDYLPAFLDSVPAPYRRAGHWIDEL
                     EGLLGEIEVRPVPIPHDCHDGFYYAYWRRPHAFLDQRVRNGISIFHQLPEAAVAEALD
                     RLRRDLDDGTWDERYAELREQEELDVGLRLVVARVG"
     gene            16960..17415
                     /locus_tag="VHF88_02930"
     CDS             16960..17415
                     /locus_tag="VHF88_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2160752.1"
                     /translation="MEVRRAGSEDAEAIGRLLHDFNVEFDEPTPAPEALAKRMRELIA
                     GGETAVLLAGDGPDGIAVLRFRPAIWSEALECYLAELYVVPAHRGHGLGRALMESAIE
                     LARSEGANYMDLGTSEDDTAARALYESLGFSNREGRPEGPVNYFYEREL"
     gene            complement(17437..18372)
                     /locus_tag="VHF88_02935"
     CDS             complement(17437..18372)
                     /locus_tag="VHF88_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160753.1"
                     /translation="MRRPLPLALALVTALLIAFPPAAAADGPIIRYECTYQPAGGEWW
                     NIPDCKPNVTPWISVGKLGHSGWKLYCPGGAPFSYQNVLNAGFAFTSNSTDWSRSSKW
                     IDAPTATGSRYTGNGKYPDWLYYKGQTLNPGDFSSGWVDAFVVNWDPFHDHSWRYAMG
                     CAATNAVEDPSGVQVGPIPGGPDGDDALDYDNVVATADPPAGAARTAQSSDGPAAPQR
                     RSIVAVRRDGRIERLRELPLRPRRTTRVTVRCAKGERLVRGSHSVAYDTRRPPRTHRH
                     NDVAGRTRRAYSVRVKVGRVAPRTVWLQARAICQR"
     gene            18497..18868
                     /locus_tag="VHF88_02940"
     CDS             18497..18868
                     /locus_tag="VHF88_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX2160754.1"
                     /translation="MRRLALALPETAEGLAYGHAAWRVRKKVFVWERPLRKTDLAALG
                     DAAPSGPVLGAWVEHVGAKEALLATEPGLFFTTPHFDGYAIVLVRLDSIGLEGLEEVV
                     VEAWLARAPARSARIYLDAAR"
     gene            18884..19309
                     /locus_tag="VHF88_02945"
     CDS             18884..19309
                     /locus_tag="VHF88_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX2160755.1"
                     /translation="MSDPKLPLTGGCGCGAVRFEVTAPLVSALYCHCTRCQRRSGTAA
                     SANALPEPGSFRLLSGEEALRAWKPGAGWEKWFCGECGSAIFSRAPHDPALMSIRMGA
                     FDSDPGVRPSARQFVEFAATWEPIPDDGLPRYPRSAAEG"
     gene            19516..20283
                     /locus_tag="VHF88_02950"
     CDS             19516..20283
                     /locus_tag="VHF88_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160756.1"
                     /translation="MRHKHRIAALAACAASIFAVSASPALAQSLSLTGPSSATVGQPV
                     TLQASGTVPEDAFLTRYINAYAIPTSALPSCPATYQGAIQAKDAAAAQGGDTVAVVVP
                     VEGNFSVPILYTGKVPGQFLICAYLHELAVTEAVASHAISVAAQGGGGGGTPPPSGGP
                     VVGGDDDAKPPRPAVVKKPKVKRARGKLVCNRGRWSGNPTRFAFHWKVNGKRKAGAKR
                     KTLKITDSLEGKSVRCGVKAIGPGGSRTVVSPSVRVG"
     gene            complement(20360..21211)
                     /locus_tag="VHF88_02955"
     CDS             complement(20360..21211)
                     /locus_tag="VHF88_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2160757.1"
                     /translation="MSDALTIAGYALREAVRRRVLLVVALLTAVFLGLFALAAFEAFD
                     SIEQSGGGIPLDERALAGGTLLGLAMFVALFLGVVLAVFLTLDAVRGDAERGLLQPLV
                     VRPVGRTTLLAGRLLAASAVCASYVVVVFLAAAAITHAAGDFTPSSLVVPAAGLALGV
                     VTVVAVSLLGSTVLSTTANGIAVFMVFATGLAAGLLGQIGEGLQVDALETASSIMSWI
                     VPFEALYQNGLYALTSDIGGATGVIVQLGPFGGASDVSTAIWVWAVAWSAVVLTLAAL
                     AFKRRDL"
     gene            complement(21208..22053)
                     /locus_tag="VHF88_02960"
     CDS             complement(21208..22053)
                     /locus_tag="VHF88_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2160758.1"
                     /translation="MTTPALSARALSKRYGRTTALDAVDLEVGRGELVGLLGPNGAGK
                     STLVKIACGLVRPTAGEAHVAGAPAGSPAARASTGYLAELFRFPAWATADEVLKLHQR
                     LARSDGGPAEREALLARVGLSGEGGRRMATMSKGMQQRLGIAQALIGDPRLVLLDEPT
                     SALDPRGRRTVRDLLVSLREQGVGVLLNSHLLSEIELVCDRVVIIDGGRVVTEGRPED
                     LTAPGGVEVETAAGVRRLPDARREDVPRIVRELVAAGDEVYGVRVVTGTLEDAYLAAV
                     VGRDG"
     gene            complement(22068..>22468)
                     /locus_tag="VHF88_02965"
     CDS             complement(22068..>22468)
                     /locus_tag="VHF88_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160759.1"
                     /translation="RELLGVFDALLLVVLGLVLYAMTAREPARRPGLMDRVQLLAVAS
                     ALVLDLMVLGAMVARIGDLGFTPNRAAALGLNLVLLVNLAGAAWLQLRFLARRVQFHR
                     LERWQTSYLPFFALWAATVVVALPPLFSFD"
BASE COUNT         3008 a         8202 c         8198 g         3060 t
ORIGIN      
        1 cacgagcctc gagcgccagg tcggcgacga ggggcagacg gagttcggcg agctgctgcc
       61 gtcggaccgg ccgccgcccg acgaggaggt cgagatcgcg ctgaccgagc agagcctgcg
      121 gcgcgcggtc gcccagctgc cggacaacga gcgccaggtg ctgcggctgc gctacgggat
      181 cgacggcgcg gagccggtcg ccttgcgaga ggccgggcgg cggctcggcg tgtcgtcgga
      241 gagcgtgcgc aacctcgaga aggccgcgct gcgccacctc gccgagaacc gcgaggtcgc
      301 ggcgctcggc caggccgccg cctgagcggg tcgcggctgc gctagcttcg ccgcgtgcgg
      361 gcgacgacgg cgatgggctg cctggcggcg gcggctctct cgattttcgt ggtcgccccg
      421 gcgcctgcgt acgacgcgtg ggcgtggctg ctgtgggggc gggaagtcgc cggtggggcg
      481 ctccacaccg ccgaggggcc ggcgttcaag ccgctgccgg tggcggtgtg cgcgctgctg
      541 tcggcgttcg gtgacgcggc ggcgctgctc tgggtgatcg tcgcgcgcac cggcgcgctg
      601 ctggctgtcg tactggcgtt ccgcttgggg tggcggctga tgggcgaggc gttgggcgcc
      661 ggcggcgctg gccgctcgcc tctggtgggc ggtctgctcg ccgcggccgg ggtggcgctc
      721 tgcggctcgt tcctcggcta tgcggctgag ggagtggtga ccggctggct ggtggcgctc
      781 gggttggccg ggctggaggc gtggcgcgcg ggtcgtccgc gcgcggcgct cgcctgcgcc
      841 gtcgcctgct gcctgctgca cgtcgaggcg tggcctttcg cggcggcgtt cgggctgttc
      901 gcctggttgc gccggccgag cgatcgcccg ctgctggcgg tcgcggcgat cgccgtgccg
      961 gcgctgtgga tcgtccccga gtggatcggc tcaggtgatc cactgcgctc aggcgctcgc
     1021 gcacgcattc cgaacccggg acagccggcg ctcgcggacg tgccggcgct ggcgtcgctg
     1081 gagcagtcgg cgcggctgct gctgtggccg ctgtggatcg gaatcgcggc gctggcgttg
     1141 gctgcctggc ggccgagtcc tggcggtggc ctgccgccac gcgcctggtc cgccctcggt
     1201 gaagggctcg gaggcgccgc ggcgctcgcg gcgatcggga cggcctgggt cctcctggtc
     1261 gccttgatgg cgcagttcgg cggcttctca ggcgagccgc gctacgcaac cccgggcgtc
     1321 gcgctgatcg cgatcgccgc tgcggtcggc ctggctagcg gtatcgccgc gatctcgcgg
     1381 cgcgcgttcg gccgcgcttg gatcgccgcc gccgtgttgg gatcggcgct cctcgtcgcc
     1441 ggcatcgcgc gcctcgacca gctgtccgcc gtgccggcgg cgcaggcgca ccaatggcgg
     1501 ctggcgaacg acctgagcga ggcagtcgag gccgcgggag gtcgcgacgc ggtgctgagc
     1561 tgcggcatcc cttacgtagg accgctgcgc gggccgctac tagcccatcg gctcgacgtc
     1621 gcgaaggagg tcgtggagcc cgacctgcct cctcgaccgc ccgcggtggt cttccgctcg
     1681 ccgcgaaggg ccggagccag ggtgactccg gcagcgcgtc ccgtgttccg ccagatcgcc
     1741 gtcgtgggcg actggcaggt cgctcgggcc tgcagagtta ggaagcccta acatgcccaa
     1801 ccgctttgcc ctggcggcct cccctcatca cgatcctgct cgccgtcgcg ctcgccggct
     1861 gtggcggcgc tgacgccaac gaggagatcc ccgacccgga gcgcgagcca gcgaccgagg
     1921 ccggcaaggc gcgccatttc gacgtcgcgc ggatcgcgac cggcctcaat cgcccgacct
     1981 tcgtcggcgc cgcgcccggt gacaccggag cgctgtgggt gctcgagcag ccgggacggg
     2041 tggtccggct cgggccggat ggcggtcgcc tggtcgcgct cgatatctcc gatgaggtga
     2101 aggtcggcgg cgagcagggc ctgctcggca tcgccttcca tcccgacttc gcgaccgacc
     2161 gccgcgtcta cctccactgg agtgacccgc agggcgacac gcgcgtcgcc gagctggagg
     2221 cgcgcgccga tgggacgatc gacggcggcg cgcgccgcgc gttgctgcgc ctggaccagc
     2281 cggaggagaa ccacaacggc ggccagctcg cgttcggtcc ggacggccgc ctctaccttg
     2341 ggctcggcga cggcggcggg tcgttcgacc cgaacgagac cgcccaggac ctcgactcgc
     2401 cgctcggcaa ggtcgcgtcg ctcgacgtgg acgccccccg gccgcgctgg cgcacggagc
     2461 tgtacgggct gcgcaacccg tggcgcttct cgttcgaccc ggcgctcggc gagctgttgg
     2521 tcgccgacgt cggccaggac gagaccgagg agatcaaccg ggtgctggtc gagcccgacg
     2581 agccgccgaa gaacctcggc tggagcgcct tcgagggcac cttccggatt cacgagacgc
     2641 acgagctcga cacccgcggc gagctgatct ggcccgtcgc gacctacagc cacgacgacg
     2701 gctgctcggt caccggcggg ttcgtctacc ggggcaccgc cctgccgcgc ctatcgggcc
     2761 gttacgtcta cggcgacttc tgcagcggct ccctctggac gttgaagccg acccccgacg
     2821 ccttcgccga ggacgtccgc cgcgagcagg ccgccgtccc gcagctcacc cacatcggcg
     2881 aggactccga gggccggatc gtcttcgcgt cggcgaccgg cgcgctctac cgcgccgcac
     2941 cgccgccgcg ctgagccgag cgcgggtcgc cggcggctgg gtcgccggcg accgcttcgc
     3001 tcgtcatcgg cctgctagcc ggcggcccgg cgcagcgcgc cgcccccggt cagcagcgac
     3061 aggccgatca gcgccaggac ggcgatggcg agccccgtca tcggcagctc gccgctcgcc
     3121 atgtcgccgg ccacgtcgcc ggctgcgacc gggatggtcg agccggccgg cgccgaagcg
     3181 ccgtcgggca gcgtgccgcc gtccgggagc gagtcgccgt ccggcgccga gcccccgtcc
     3241 ggcgccgacc cgccgtccgg gcccgccccc ggagagggga cggcgaccgg atcgcgatcc
     3301 ggggctcggt cgaccgccgg gacggttccg gccccgagga cgtcccctgg cgccggcgag
     3361 ccgttccgcg acccggcacc gggcgcggga tcgtcgaccg ggtccgggac cgccgaggcg
     3421 gtcgctccgt cgttcccccg actggcgatt cggagcggcg caccagccgc cgcgccgccg
     3481 accgcgaggc caccaccgcc gtcgccaacc gtctgcgaac cggccccggc gccgggatcc
     3541 gacccagcgt cgtcgtcacc agtgctcagc actcggattg gcgctgccgc gcggaccggc
     3601 gccacctgcg caccgccggc cgagtccctg gcggtctgct cggtcccgct gctcgtccct
     3661 tgcgtcgaac cgctggcgtt gtcgccgtcg ctcaggacgc gtaccggtgc atcggcatcg
     3721 acagcgccca cctgggcacc gccggtcgag tcgtcgacgt cctgcgccgt cgtcgacgat
     3781 ccgtcgccgg cggcggtgtc gtcgccgtcg ctcagcactc gaacgggtgc gtcggcgtcg
     3841 accgagccga cctggccggt cacggccgag tcgctcgtgt cctgcggtgc ggccgtcgac
     3901 ccgcctccgt cggcagcggc gttgtcgccg tggctgagga cgcggaccgg cgcgtccacg
     3961 tcgacggcgc cgacctggcc cgtcgccgtg gagtccccgg tctcctgcgg tgcggccgtc
     4021 acgcctgccg gcgcggcgtt gtcgccgtcg ctgaggaccc tcacgggcac gtcggcctgg
     4081 accgcgccga cctgggctgc gcccgtcgag tcgcctatct gttgcccggg gtcgcccgtc
     4141 tgggcagccg cgggcgctgt atggagcgcc agtgcgagca ggctcgcacc cagcgccatc
     4201 gcgctgcgtc tcattgtgtg ctccttccgt tggatcgaac tgcggcgagc gcgcgaaggc
     4261 gccgcctcgc gtggtgacgc cgcccggcta gccgggccgc tcgatccgaa cgcggaacgc
     4321 cgccgccccc cagcgggtcg gcgacatctg gagaaggcgc gaggtgaagc gggggacaag
     4381 ggtcagcagg gcgacgagca gtccgagccc gatcagcgcg atgccgcccg gcccgccgag
     4441 cagcccctcg ccgccgggcc cgaagggcac ctggtcgccg tcgccgtcgt cgccgaaggc
     4501 cgaggtcgag gaggcgtccg cgcgagccag ctcgccggcc gcgatcgccg ggagggtcag
     4561 cgtcccgagg ccggcgcttg ccgtgagcgc gtcgacggcg tcgtgggcga taccgcccgt
     4621 agccgagtct ccggaccgcg ggctcgcctc gcgatcgatt cgtccgatgt cgcgtcgggg
     4681 cgtgccgtcg gctcgatcgc cgtggccggc gccacccgtg cgcggctgtg ccgcactacc
     4741 gccatgccgg acgccggtcg agacaccggc cggcgctccg ccggtggcgg ccgccgccac
     4801 gggcgtccgc acggcatcgg ccgccggtcg gatcggctcc accgccgcca cccgctccac
     4861 cgtctcgcga accggctgcg tgaccgcctc gaccgtcgag cggaccggcc tggccgcccg
     4921 caccgtgtcg accgtcgcct ggaccggtgc ggtcgtgcgc tcgacggtct gctggacgac
     4981 cggcgccgcc gcctggacct gatcgtcgac cgcctgggtc accggcgcgg ccaccttcgt
     5041 taccggttgc gtcaggccct cgaccggccc ggccgccgcg ctcgcgggcg tcgccgccat
     5101 cccgagcacg agcaccagcg tcacgcgcca tcggtgccga tagcgacgag gtcgggcgaa
     5161 cgtgactcga tacagcgatc ccatctaggg aactggctgc ccgatcccgg gtctacggcc
     5221 gtcgaagtgg ccgaactgcg gaggcttcgg ctcggagcct gtgcatcatg cgttgtaacg
     5281 gcgggcgcgc ctacgtaacg gccaggcgac ggctgcgcca ccactcgcgg atgaggatct
     5341 ggatggtggc ggtggcgggg atggcgagca gcgcgccgag gacgccgagg agggtggcgc
     5401 cggccatcac ggacaggatc acgagggcgg ggctcatgtc gacggccttg cggtagacga
     5461 gcggaccgag gacgtgggtc tcggtctgct ggtagaagag ccagaagaca gcccagatcg
     5521 cgacgatctc ggggccctcg aaggcggcta cgacggcgat cacggcggcg ccgatcgtcg
     5581 agccgacgag cggcaggatc gcgaagaagg ccatctggac ggcgagcggg acggcgtagg
     5641 ggattccgag caccgtcatg accaggaacg acacgacgcc tgccagcgcg gcgacggcga
     5701 acacgccggc gacgtagccg ccgaccgccg acgacgtctc cgcggcgacg cggcgggcct
     5761 tggcctcgtc gggcgcgacc tcgcggtaga cgaagccgag cagacgcggc gcgtcgagca
     5821 gcagcaggaa tgcgatcgcg aggatcgcga tcagctccac gacccgctcg atcgcggcga
     5881 cagtgacggt ctcgagctcg ctcagcgcgc cgccgaggat cgacggcagg ttgcgcgcct
     5941 cgccctccag atgggcgacg atcccgtact cccggtccca gcgctcgatc aggccggagt
     6001 cctggacatc gcgaacgtag ccgggcaccg cgcgcaggaa cgcggtgaac tccgtgatca
     6061 gcggcggcag caccagcagg ccgatcccga ccatgaccgt cgccagcacg acgtagacga
     6121 ccaggatcgc cagcgcgcgc ggcagcccca gccggcgctg gagcaggttc acgggcgcgc
     6181 tcagcgcgac ggcgaggaag atcgccagga tcgccagcag gagcacgtcg cgcagcagcc
     6241 aggcgacgta gatcaggccg acgatccccg ccgtcgtcag caccgtgcgc gccgtctggc
     6301 tgacgccgat cgtccgccgc gccggctcgc cgcccgctgt cgcgtccatc cacggctact
     6361 acgccgcacc ttccggcttc cctcctgcag gactccggtc aaccccccga tttctcggcg
     6421 gcggcgcgcg gccttgctac tttcgagcgc ctgcccggcc gtctccgact cgcgaggtcc
     6481 atatgtctct cctgctcgcg cgcctcgcgc gcacgatcac ccatcactgg aagcgcagcg
     6541 cgctagccgc agtcgtcgcc gtcgtcctgc tcggggcggc cgccggcgcg ggcggtcagg
     6601 cggccgacga cttcaacgtc ccagggaccg agtcgcagca ggcgctcgac ctcttccgcg
     6661 agcacagccc gacgtttgcc ggtgccgact cgacggtcgt cttcagcgtc gagcagggca
     6721 cgctcgatga gccggagcga cgcgccgcga tcgaggaggc ggtcgccgag gtccgcgcgc
     6781 tgcccggcgt caaggagttc ggcgacccgt tcgccgaggg cgcgacgatg tcggaggacg
     6841 gccggatcgc cgcggtcgac gtccgctacg acgtcgagcc cgacgacatc gagcgcgagg
     6901 acggcgaggc gctcgaggag gccgcccgga cggcggaggc tggcggggtc gacgtctcta
     6961 tgcgcggcat cgtcgtcgac atcgcctcgg agcaggaggc gccggtcggc gagctgatcg
     7021 gcgtcgcgat cgcgatcgtg ctgctgacgc tgctgttccg ctcgctcgcc gccatgttcg
     7081 cgacgatcgt cggcgcgctg atcggcgtga tgaccggcca gatgctgctc gccgcgctcg
     7141 ccaagccgct cgggctgccg gagttcgcgc cggtgatcgc gatgatgctc ggcctcggcg
     7201 ccgggatcga ctacgcgctg ctgatcatcg gccgctaccg cgagcaggca gccgccggcg
     7261 acagcacgcg cgatgctgcc gcgaaggcgg ccggcacgtc tggcgcgtcg gtcgtcgccg
     7321 cgggcctgat cgtgatggtc gcgatcgccg ggctgctggt cgtcggcatc ccgtttatcg
     7381 gcaagctcgg cgtcgccgcg gcgcttgcgg tcggggccgt cgtcgtgtcg gcgctgacga
     7441 tcctgccgat catggtcggg gcgctcgcgc gctggctgaa gccgaagaag cccgagcacg
     7501 tgctgccgtc gcgcttcttc tcccgctggg gcgagatcgt cagcgggcgg ccttgggtgg
     7561 cgatcggcgc cgcggtcgcg ctgctgctgg tgctcgcgtt ccccgtgacg cagctgcgac
     7621 tcggccagcc cgacgacggc aaccagccga cggagaagac gcagcggatc gcctacgacc
     7681 tccagagcga ggcgttcggg cccggctcga acggcccgtt tctgctcgcg gtcgacgtgc
     7741 cgcggcacgg cgccgacacg aacgcccaac taggccggat cgaggctgct gtgcgcgaca
     7801 cgcccggcgt ggcgagcgtc gcgcccactc agctcagtca ggacggcgag atggcgacga
     7861 tcttcgcgat cccgaccacc gcgccacagg acgcggcgac cagcgacctg ctcgagcggc
     7921 tgcgcgagca gacgatcccg gcggcgaccg ccggcacgcc gctgaaggtc tacgtgggcg
     7981 ggaacacggc gaccttcgag gacctctccg acaaggtcgc ctcgcggctg ccgctgttca
     8041 tcgcggtcgt gatcggcctg tcggtgctgt tgctgatcgc tgcgttccga tcgatctggg
     8101 tcccgctcgc ctcggcggtc atgaacctgc tgtcgatcgg cgccgcctac ggcgtggtca
     8161 ccgccgtctt ccaggagggg gtcggcgccg gcctgctcgg cgtcgacagc ggcgtgccga
     8221 tcgtctcgtt catcccggtg atgatgttcg cgatcctgtt cgggctgagc atggactaca
     8281 acgtcttcct gctctcgcgg atccacgagg cctacaacga gggcgacggg ccgcgcgcga
     8341 gcgtgatcca cggcatgggg cggatcggca aggtgatcct gttcgccggc ctgatcatgg
     8401 cggcggtctt cctcgccttc gtcagccagc cggacatcat cggcaagatg atgggactcg
     8461 gactaggcgt ggcgatcctg atcgacgtcc tcgtcgtgcg gctggtgatc gcgccggccg
     8521 tcgtcacgct gctcggcgac agggcgtggt ggctgcccgg ttggctcgat cggatcctgc
     8581 cgaacgtctc gctcgagggg cacctcgtgc agagcctcga cggcaagcag gccgacgggg
     8641 acaaggcgac ggaagaggag cgcccggtcg aggcgccggt ggcctgagcg gctaccggcg
     8701 cagcagcgcc atgacctcgt cgtcggatgt ctgggtgaag tcgtcgtacc aggcgccgac
     8761 gccgccgaag cggcgcggct gctcgaggca gaccagctcg ccgagctcga cgcgcaggcg
     8821 tccgaccgcg ctcgccgggc agaccgggac ggcggtgatc acgcgcgccg cgccgcggtc
     8881 gcgcaggccg cgggcggcgg cgacggccgt gacgccggtc gcgatgccgt cgtcgacgag
     8941 gatcgccgtc ctgccgtcga cctcgatcgg cggccgattg ccgcggtaga ggcgccggcg
     9001 acgcgcgagc tcgacggcct cgcgctcgac ggccgcgtcg atctgcgcgc gcctgacgcc
     9061 gagcgccgcg accgtctcgc gatcgacgat cacggctccg tcctcgccga cggcgccgat
     9121 gccgagctcc ggctggcccg gcgcaccgag cttgcgcacc agcgcgatgt cgagcggcgc
     9181 cccgagcgcc tgcgccacct cgtaggcaac cggcacgccg ccgcgcggga gcgcgatcac
     9241 gaccggcgca tcgagatcga gctccaccag ccgcgcggcg agcagctggc cggcggtccg
     9301 gcgatcttga aaggaggatc tgcgcgccat gtcgattcct gagcttgccg ttcgtcgcgt
     9361 gggtagagac ccgactacac cagggagtcc acgatgcgat tcatgatgtt catgctgccc
     9421 ggcggcgagc tgcaggagac aggcgactgg ggcccgccga gcgccgacga cgtcggcgag
     9481 atgacgaagt acaacgagga gctgtccaag gcgggcgtgc tgctggcgct cgacgggctg
     9541 cagccgccgt cgaagggcgc ccgcgtgacg ctcgccgacg gggcgaagca ggtgaccgac
     9601 ggcccgttcg ccgaggcgag ggagatcgtc ggcggctact ggatcatcga cgtgaagtcg
     9661 aagcaggagg ccgtcgagtg ggccaagcgc tgcccgctcg agcccggtga cgtgatcgag
     9721 gtccgccagg tcttcgagat gtccgacttc cccgagtgag ccactccgac gccacccact
     9781 ccatcgatgc cgtctggcgg atcgagtcgc cgcggctgat cgccgggctg gcgcggatga
     9841 ccggcgacgt gggggtcgcc gaggacctcg cccaggacac gctggtggcg gcgctggagc
     9901 ggtggcctcg gtcaggcgtc cccgacaacc cgggcgcctg gctgatggcc accgcgaagc
     9961 accgcgcgat cgacctcttc cgccgcaacg agcggctcgc gcgcaagcag gaggagatcg
    10021 cgcgcgacgt cgggttgcag atggcgatgg acggcgccga cctcgacgcc gcgctcgacg
    10081 acgagatcgg cgacgacctg ctggcgctga tcttcacctg ctgccacccg gtcctctcga
    10141 ccgacgcgcg cgtcgcgctg acgctgcggc tgctcggcgg actgacgacg gacgagatcg
    10201 cccgcgcctt cctcgtcgcc gagtcgacgg tggcgcagcg gatcgtgcgg gcgaagcgga
    10261 cgctcggcgc ggcgaaggtc ccgttcgagg tgccgcgcgg cgacgagctc gcgcagcggt
    10321 tgccgtcggt gctcgaggtc gtctacctgg tcttcaacga gggctacgcc gcgaccgccg
    10381 gcggcgactg gacgcgcccg ggcctttgct cggacgcgct gcggctcggg cgcatcctcg
    10441 cggggctgac gccgaaggag cccgaggtcc atgggctgct cgcgctgatg gagatccagg
    10501 cgtcgcggct cggcgcgcgc agcggccccg gcggcgtgcc ggtgctgctg ctggaccagg
    10561 atcgctcgcg ctgggacccg ctgctgatcc gccgcggcct ggcggcgctc gaccgcgctc
    10621 ggcagctcgg cggcggcctc ggcccgtacg cgctccaggc ggcgatcgcc gcctgccacg
    10681 caagggcgcg caccccggag gagaccgact ggacgacgat cgccgcgctc tacgacgcgc
    10741 ttgcccagct cgccccgtcg ccggtcgtcg agctcaaccg cgcggtcgcg gtcggcatgg
    10801 cgttcggacc cgaggccggg ctcgcgctcg ccgaccagct cgcggacgcc ccggcactcg
    10861 agcgctacca cctgctcccc gccgtccgtg gtgacctgct ggcgaagctc ggccgccacg
    10921 acgaggcgcg cgtcgagttc gagcgcgccg cggcgctgac cgagaacgcc cgcgagcgcg
    10981 cgctgctgct cgaacgggcc gcttctcgct aacttacgaa aggtgagcgt ccctctctcg
    11041 atcctcgacc tcgccccagt ccgctcgggc gagagcgcca gggacgcgct cgccggggtg
    11101 accgaggtgg cgcgtgccgc cgacgcgctc ggctaccgcc gcttctgggt cgccgagcac
    11161 cacaacatcc cggcggtcgc atcgacggcg ccggaggtgc tgatcgccca cgtcgccgcg
    11221 aagaccgagc ggatccgcgt cgggtccggc ggtgtgatgc tccccaacca cgcgccgctc
    11281 gcggtggctg agcgttttgg gatgctcgag gcgctgcacc cgaaccggat cgacctcggc
    11341 ctcggacgcg cgccggggac cgacatgcgc acggcgctcg cgctgcgcag cacctcgccg
    11401 gagagcttcg ccgaccagct cgcgcagctg cgcggccacc tcggcgacgg ccacgcgggc
    11461 atacgcgccg tcagcggcga gcacaccgtg ccggagatct ggatgctcgg ctcgaccgac
    11521 ggcggcgcgc ggatcgccgc cgcgctgggt ttgccgttcg tcttcgccca ccacttccac
    11581 ccgcacttca ccgagccggc gctgcgcatc taccgcgaca acttcgagcc gtccgacctg
    11641 ctcgaccgtc cgcgcgcgat cgtcgccgcg gcgatcgtcg tcggcgagga cgacgccgac
    11701 gccagccggc tcgcgctgcc ctcggcgatc tcgatgctgc gacttcgcca gggacggccg
    11761 gcgccgctgc cgaccgtcga ggaggcggag gagctgctgc cgcggctcgg gccgaacgag
    11821 tggtcggtcg tccaggacac gctcagccgc gcgatcgtcg gcagctcggc gacggtcgag
    11881 agccgcctgc tcgacctgat cgagcggacg gcgaccgacg agctgatgct gacctccaac
    11941 gtcgccgacc cggcgctgcg gatccaggct ttcgagcaga tggcccacgc cttcgcgctc
    12001 agccccgccg cggtcggctg agcaccgcgt cccggggcgc gcctgcctac ttcgaaagga
    12061 acgcctcgcg acccgcctgc gctcggaacg gctccagctc ggcgcgctga cgctcggcgc
    12121 gctcgatcag gtggtcgaag tggacgtccg gcaggcgctc ggcgaggccc gcgaagtcgc
    12181 gcaggttcgc ccagaggatc cgcttgccct cgatccccgc cgcgaggaag tccagctcga
    12241 cgaagcggct gagcggcgag tagctgaaca accggccgtt cagcttgagc cgcccgaccc
    12301 gctcgccagc gatcgcgagc gtcgccttga cgcggctgcg ctcgatcccc agccggtcca
    12361 tcagccgctc gaacgtctcc acgtcctcgg cgatctgcgt cgcgacccgc tccaccgctt
    12421 cccccagcgc ggtgccctcg ttgctccccg ccgaccgctt cgccacctcc cgccaaatca
    12481 cacccatcgc aagctggtcg ttcatgtaga tcccgagcag ctgcatgtct gtctgtaccg
    12541 ccggctgtgt catgcaacgg tgttacccac gagccagtcg tccaaccggg tgccgcgcgt
    12601 agcctgtggc gatggcgttc gtgccgaccg cccgtcacct gctgcgcgcg aaggacctgg
    12661 ccgacgcgcg ctacttcgag ccgctcgacg tcgacgagct cgcgggcgcg gcgggactct
    12721 ctcgcgccca cttcagccgc gagttccggc gcgcgttcgg cgagtccccg catgcctacc
    12781 tgctgacccg ccggctcgag cgggccgccg acctcctgcg caacacagac cgctcggtcg
    12841 ccgatgtctg cctctcggtc ggtctgcagt cgatcggctc gttcacgacg agcttcaagc
    12901 gcacctacgg gctgtcgccg accgcctacc gcgccaagtt cccaccggcc gcgcagcgcg
    12961 cgctcgtccc tgcgtgcgtg gtgcgcctgc gcggccgtcc gcaacgccgc acgtttcgag
    13021 aagacagccc cccgggctcc gactagcttg cctcccgaca gtcgaatcgc gaccgaggag
    13081 aaccgatgat caggatcgcc aacgctcagc tgtgggtcca cgaccaggac gaggcgctcg
    13141 ccttctacac cgagaaggtc ggcatggagg tccgcgccga cgtgacgatg cccgagatgg
    13201 acggcttccg ttggctaacc gtcggcccgg ccggtcagcc cgacgtctcg atcgtcctga
    13261 tgggcgtccc cggcccgccg gtcttcgagc ccgagaccaa gcgccagatc gaggagctgg
    13321 tcgccaaggg cgtcgccgga accgtgttcc tgaccaccga cgactgccag gcctcctacg
    13381 aggagctcag cggccgcggc gtcgagttcg tcgacctgcc gaacgagcag ccctacggca
    13441 tcgactcctc cttccgcgac ccgtcgggca accacctccg cctgatccag ctgagcgagg
    13501 tgccggtcgg gggctagacc agctggcaat ggctcaacgg cggagcgagt gcgtcggtca
    13561 gcggggctga cgcgctgcgg cgagcagctc gctgcggtcg aagaagacct cgttgcggta
    13621 gatccggtcg ccccgcagct ggaccgtgca tacgccgcgg agggtcagcg tgcggggacc
    13681 ggccggaatg tcggcctccc agtggttgag gaagccgtcc tcgagcggcg tggcccgatg
    13741 ctgccgccat tcccagtctg ggtggcgcgc gagtaggcgg cggaagtagc cgagcagttg
    13801 ctcctgtccg cgaacgccgt cggggactgc agggtcggcg tagaaggcgt cgtcggtgta
    13861 gaagctcgcg agcagctcgg gccggttgcc gctccaggcg ggcagccagc gcgttgcgaa
    13921 ctcgcgcgcc tcctccggtg tccacgcccc ggccggagct cgcggcccgc gacccgtcaa
    13981 ggctcggtcc ccctcgtccc gccgaccgct gcggtgctgg cgacgacggc ctcgatccgt
    14041 gcgagctcgg gacgcttcgc ggccatgccg tcgcccgacg agcggccgcg cacgcggcgg
    14101 aggatccatg gcgccatgtg gcgtgccgtc cagatcgcgt ggccggctgc ggcctgcgcg
    14161 cggcgcagcg actcggccgg tgggagcggc gcccagggat ccgcgtcggg ctcgaggccg
    14221 agggcctgag cggcggcgtg agcgagccgc tcgtggccga ccgagctggc gtggaggcgg
    14281 tcgtcggacc agagccgcgc gtcgacaatc ccgtcgcgct cgacgtcgat gagcagcgcg
    14341 ccgtgccgga cggcgatctc ccgcagcccc tcgttgaacg cgagcactcg tgcccgggtc
    14401 ggccgcgcca ccggcatgac gtgcgtcggg tcgggatagg tcatcccgac gacggtcgcg
    14461 ccggcgtcgc gcagcgcgga gatcaccgcc tcgatctcgg cgagcacgta gcgcaggtcg
    14521 gctcgcgggc gcaggatgtc gttgatgccg cccacgacgg tcgcgagatc tggctccatc
    14581 gccagcgccg gcttgagctg ctcggcgcga atttggcccg ccttgcgccc gcgcaccgcg
    14641 aggttggcgt agagcaggcc gggatgctcg cgcgcgagtc gggcggcgag gcggtcggcg
    14701 aagccgacga acccgtcgcc gccgggcgcc gggtcgtcga gtccctccgt tgtgctgtcg
    14761 ccgagcgcga cgtagcgtga gaacggcatc gacagagtgt tctacaagtt ctcgcaccac
    14821 taccggagga gctcaccatg gcaatcgcga ccgcaccgat aacgggcgtc gacttcgtct
    14881 gcatcccgac gcgcgaccac gaccgcgcgg ccgagttcta cggcggcacg ctgggactac
    14941 cgaaggtcaa gcgctggggc gacatgccgg cgacggagtt ccaggccggc aacctgacgc
    15001 tcgccgtgat ggatccgagc gcgttcggca tgcccgaggc caccccgaac agcacgccgg
    15061 tcgccttcca ggtcgacgac gtcgaggcca cgcgtaagca gctcgcggcc gagggcatcg
    15121 agttccaggt cgagctgctc gactccggcg tctgccacca ggcgatcttc agcgaccccg
    15181 atggcaaccc gctgatcctg caccaccgct acgccgactg accgcggccg tccgctcggg
    15241 tcaccgcgac gacgacgccg ccagcgctcg cccctgcgcc aggccggcgg cgagcgtcgc
    15301 ggcgcgcagc cgcccgtcca tcagccgtcc cgccatcgcg acggtcgagg cgggatcggg
    15361 tgcgacgacc tggacgcgaa cgccgcggcg ttcgaggtag gcggcctcga tcgcggccag
    15421 cgagcgcgac aacaggccca tcgcgccgta cggctgctcg cggctcggcg ttagcgacgc
    15481 cgtggggttg aggcagagaa cgcgcgaccc ccgctcggct ggcgcccggt ccatgttcgt
    15541 cgggctccag acgccgccgt cgaggtagcg ccgcccctcg atctcgatcg gccggaagaa
    15601 cccggggatc gcgcacgagg cctggacggc cttcgcgatc gtcgcctgcg gcgctccctg
    15661 ctcaccgaat atcacgcggc ggccgctgtc catgtcgacc gctgagatgc gcaggcggcc
    15721 gtcccagtcg atgccccagt cctcgacgac cgccgcgagc tgcgccagcg agcgccggcc
    15781 gggcgtgacc cggctgagcg ccttgcgacg cagcagcgct ccgccgggcg ccatgaacgg
    15841 cagcgtgagc gcggcgagcg gaccggccac cttgcccgcc tgcccgagcg cgcgcgccag
    15901 cggccgcggc gcctcggcgc cgaccgcctc gtccgggacg tcgggctgct ccggcagctc
    15961 gtcgagtcgc gagctggggt cgacgcccgc cgtcagcgcc gcggcgacga tcgagccggc
    16021 cgaggtgccg agatacgcct cgcagccgtg cggatcgaag tcgctcgcct cggccaggcc
    16081 ggcgagcacc gcgctcatcc acgcctcgcc gaggatccca ccgccgccca ggacgaggat
    16141 gtccggctgc tcgaatcggc tcatgggacc aaccatgccc aattacgatg ctttcgtgag
    16201 cgatcccgtc tacgaccggc tcggcgtcgg ctacagcgcg gtccgagcga ccgacccgag
    16261 gatcgcggcg gcgatcgacg aggcgctcgg aggagcgcgg acggtgctca acgtcggcgc
    16321 ggggaccggc tcctacgagc agctcggccg cgacgtcacc gccgtcgagc cgtctccgga
    16381 gatgatccgt cagcgcccgg cgggcgctgc ccccgtcgtc caggcgaccg ccgaggacct
    16441 gcccttcgac gacgacagct tcgacgccgc gatggcgatc atctccgacc accactggag
    16501 cgatcgcgcg gcgggcatgg cggagctgcg ccgagtcgcc cgcgagcgcg tcgtcgtcct
    16561 caacgccgac ccgagcttcg ccccgcgctt ctggctgtcg cgcgactacc tgccggcgtt
    16621 cctcgacagc gtcccagcgc cataccgccg cgccggccac tggatcgacg agctcgaggg
    16681 cctgctcggc gagatcgagg tccgtccggt cccgatcccc cacgactgtc acgacggctt
    16741 ctactacgcc tactggcgac ggccgcacgc cttcctcgac cagcgcgtgc gcaacggcat
    16801 ctcgatcttc caccagcttc ccgaggcggc ggtcgcggag gcgctcgacc gcctccggcg
    16861 cgatctcgac gacgggacct gggacgagcg ctacgccgag ctgcgcgaac aggaggagct
    16921 cgacgtcggc ctgcggctcg tcgtcgcgag ggtcggctga tggaggtgcg ccgtgccggg
    16981 tcggaggacg ccgaggcgat cgggcggctg ctgcacgact tcaacgtcga gttcgacgag
    17041 ccgaccccgg ctccggaggc gctcgcgaag cggatgcggg agctgatcgc cggcggtgag
    17101 acggcggtgc tgctcgcggg cgacgggccg gatggcatcg cggtcctgcg cttccgcccg
    17161 gcgatctgga gcgaggcgct cgagtgctac ctcgccgagc tctacgtggt gccggcgcac
    17221 cgcggccacg gcctcggccg cgctctgatg gagagcgcga tcgagctcgc gcgcagcgag
    17281 ggcgcgaact acatggacct cggcacctcg gaggacgaca ccgccgcccg cgcgctgtac
    17341 gagagcctcg gcttctcgaa ccgcgagggc cggccggagg gtccggtcaa ctacttctac
    17401 gagcgcgagc tctgaggggg cgcggagcgg ccgcggtcag cgctggcaga tcgcgcgcgc
    17461 ctgcaaccag accgtccgcg gggcgacgcg accgaccttc acgcgcaccg agtaggcgcg
    17521 tcgcgtgcgg ccggcgacgt cgttgtggcg gtgggtgcgc ggcggccggc gggtgtcgta
    17581 ggcgacggag tggctgccgc gaaccagccg ctcccccttg gcgcagcgga ccgtgacgcg
    17641 cgtcgtccgc cgaggccgca gcggcagctc gcgcagccgc tcgatccgcc cgtcgcggcg
    17701 gaccgcgacg atcgagcggc gctgcggcgc ggccggtccg tcgctgctct gcgccgtgcg
    17761 cgccgcgccc gccggcgggt cggcggtggc gacgacgttg tcgtagtcga gcgcatcgtc
    17821 gccatccggg ccgccgggga tcgggccgac ctggacgccg ctggggtcct cgaccgcgtt
    17881 ggtcgccgcg caccccatcg cgtagcgcca cgagtggtcg tggaacggat cccagttcac
    17941 gacgaaggcg tcgacccagc cggagctgaa gtcgccggga ttgagggtct ggcccttgta
    18001 gtagagccag tctgggtact tgccgttgcc ggtgtagcgg ctgccggtcg ccgtcggcgc
    18061 gtcgatccac ttgctcgagc gcgaccagtc ggtcgagttg gaggtgaacg cgaagcccgc
    18121 gttgaggacg ttctggtagc tgaacggcgc gccacccggg cagtacagct tccagccgga
    18181 gtggccgagc ttgccgaccg agatccacgg tgtcacgttc ggcttgcagt ccgggatgtt
    18241 ccaccactca ccgccggccg gctgatacgt gcactcgtac ctgatgatcg gcccgtcggc
    18301 cgcggcggcg ggcgggaagg cgatcagcag ggcggtgacg agtgcgaggg caagcgggag
    18361 gggtcgacgc atggcgcggc agtatgccag cggctccggc gcccggatag ggctctggac
    18421 aagtgttcga tctctttgtc agcggctagg ttgcggctcg tgagatcggc tcggcgcatg
    18481 gcgacctggg aatgacgtgc ggcgcctcgc gctcgcgttg cccgagacgg cggagggact
    18541 cgcctatggg cacgcggctt ggagggttcg caagaaggtc tttgtctggg agcgcccgct
    18601 gcgcaagacg gacctcgccg cactggggga cgcggcgccg tcgggcccgg tccttggcgc
    18661 atgggtcgag cacgtcggcg cgaaggaggc gctgttggcc acagagcccg ggctcttctt
    18721 caccacgccg cacttcgacg gatacgccat cgtgctcgtg cgcctcgact cgatcggtct
    18781 cgagggcctc gaggaggtgg tcgtggaggc gtggctcgca cgggccccgg cgcggtcggc
    18841 tcgcatctac ctggacgctg ctcggtgacc ataatctgcg ctcgtgagcg atccgaagct
    18901 tcccctcact ggcgggtgtg gctgcggagc ggtccgcttc gaggtgaccg cgccgctcgt
    18961 ctcggcgctc tactgccact gcacccgctg ccagcggcgc agcggcaccg ccgcgtcggc
    19021 caacgcgctg cccgagccgg gctccttccg cctcctgtcc ggcgaggagg ccctgcgcgc
    19081 ctggaagccg ggggccgggt gggagaagtg gttctgcggc gagtgcggct cggcgatctt
    19141 cagccgcgcc ccgcacgacc cggcgctgat gagcatccgg atgggcgcgt tcgacagcga
    19201 tcccggcgtc cggccgagcg cgcgccagtt cgtcgaattc gcggccacct gggagccgat
    19261 ccccgacgac ggcctgccgc ggtacccgcg cagcgcggcc gagggctagc gcggcgcttg
    19321 cgcgatgacg agctacggtg cactccggcg atgatccgca tacaggtgac agtcgattcg
    19381 gcagcttcga ctgacctcaa ccgtcgatgc ggccgagggc tagcgcacga ccgcgggtgc
    19441 ttctcaacga cccgccgccg tgcaatcctc gtcgctttcc gaacccgccc gaacgacagg
    19501 aaggaacccc gcaggatgcg tcacaagcac cggatagcgg cgctcgcagc gtgcgccgcc
    19561 tcgatcttcg ccgtatcggc gagccccgcg ctcgcccaat cgctcagcct caccggaccc
    19621 agctccgcga ccgtcggaca accggtcacg ctccaggcgt ccggcaccgt ccccgaggac
    19681 gcgttcctca cccgctacat caacgcctac gcgatcccga ccagcgcgct ccctagctgc
    19741 cccgccacct accagggcgc gatccaggcc aaggacgcgg ccgcagcgca gggcggcgac
    19801 acggtcgccg tggtcgtccc cgtcgagggc aacttctcgg tgccgatcct ctacaccggc
    19861 aaggttcccg gccagttcct gatctgcgcc tacctgcacg agctcgcggt caccgaggcg
    19921 gtcgcctcgc acgcgatctc cgtggcggcg cagggcggcg gtggtggtgg cacgccgccc
    19981 ccgagcggcg gtcccgtagt gggcggcgac gacgatgcca agccgccccg ccccgcagtc
    20041 gtcaagaagc cgaaggtcaa gcgcgcccgc ggcaagctgg tctgcaaccg cggccgctgg
    20101 tcgggcaatc cgacgcgctt cgccttccac tggaaggtca atggcaagcg caaggccggc
    20161 gccaagcgca agacgctcaa gatcaccgac agcctcgagg gcaagagcgt ccgctgcggc
    20221 gtgaaggcga tcggtccggg cggctcgcgg acggtcgtga gcccgtccgt gcgcgtcggc
    20281 tgagagacga atggcggatc gggcccttgc ctttggcgag ggtccggccg ccggctcctt
    20341 ccggcttcgg tctcgcggct cagaggtcgc ggcgtttgaa ggccagcgcc gccagcgtca
    20401 gcacgacagc cgaccacgcc accgcccata cccagatcgc cgtgctgacg tcgctggcgc
    20461 cgccgaacgg accgagctgg acgatcacgc cggtcgcgcc gccgatgtcg gaggtcagcg
    20521 cgtagagccc gttctggtag agcgcctcga acgggacgat ccaggacatg attgagctcg
    20581 ccgtctcgag cgcgtcgacc tggagcccct caccgatctg gccgagcagg ccggcggcga
    20641 ggccggtggc gaagaccatg aagactgcga tgccgttggc ggtggtcgag aggaccgtcg
    20701 agccgagcag cgagaccgcc accacggtga cgacgccgag cgccaggccg gccgcgggga
    20761 cgacgagcga cgagggcgtg aagtcgccgg cggcgtgggt gatggccgcg gcggcgagga
    20821 agacgacgac gacgtaggac gcgcagacgg cggaggcggc gagcagccgc ccggcgagca
    20881 gcgtcgtgcg gcccaccgga cgcacgacca gcggctgcag cagcccgcgc tcggcgtcgc
    20941 cgcgaaccgc gtcgagcgtc aggaagacgg ccagcaccac gccgaggaag agcgcgacga
    21001 acatcgccag gccgagcagc gtcccgcccg ccagggcccg ctcgtcgagc gggatgccgc
    21061 cgccgctctg ctcgatcgag tcgaaggcct cgaaggcggc cagcgcgaac agccccagga
    21121 agaccgcggt cagcagcgcg acgacgagca gcacccggcg gcggaccgcc tcgcgcagcg
    21181 cgtagccggc gatcgtcagc gcgtcgctca tccgtcgcgc cccaccacgg cggcgagata
    21241 ggcgtcctcg agcgtgccgg tcacgacgcg cacgccgtag acctcgtcgc cggcggccac
    21301 cagctcgcgg acgatccgcg gcacgtcctc gcggcgcgcg tcgggcaggc ggcgcacgcc
    21361 cgcggcggtc tcgacttcga cgccgccggg cgcggtcagg tcctccggcc gcccctcggt
    21421 caccacgcgg ccgccgtcga tgatcacgac gcggtcgcag acgagctcga tctcgctgag
    21481 caggtgcgag ttcagcagca cgccgacgcc ctgctcgcgc aggctcacga gcaggtcgcg
    21541 caccgtgcgc cggccgcgcg ggtcgagcgc gctcgtcggc tcgtcgagca gcacgagccg
    21601 cgggtcgccg atcagcgcct gggcgatgcc gagccgctgc tgcatgccct tgctcatcgt
    21661 cgccatccgg cgcccaccct cgcccgagag cccgacccgg gcgagcagcg cctcgcgctc
    21721 cgccggcccg ccatccgagc gcgccaggcg ttgatgcagc ttcagcacct cgtcggcggt
    21781 ggcccacgct gggaagcgga agagctccgc gaggtagccg gtcgacgccc gggccgcggg
    21841 gctgccggca ggcgcgccgg cgacatgcgc ctcgccggcc gtcggccgga ccaggccgca
    21901 ggcgatcttc accagcgtcg acttgcccgc tccgttgggc ccgagcaggc cgacgagctc
    21961 gccgcggccg acctcgaggt cgacggcgtc gagcgcggtt gtcctgccgt agcgcttgga
    22021 gagcgcgcgg gctgagaggg cgggggtcgt catcgaaggg tggcttctca gtcgaagctg
    22081 aagagcggtg ggagcgcgac gacgacggtc gccgcccaga gggcgaagaa ggggagatag
    22141 gacgtctgcc agcgctcgag gcggtggaac tggacgcgcc gcgcgagaaa ccgcagctgc
    22201 agccaggcgg ccccggcgag gttgacgagc aggaccaggt tgaggccgag cgccgccgcg
    22261 cggttcgggg tgaacccgag atcgccgatc cgggcgacca tcgcgccgag caccatcagg
    22321 tcgagcacga gcgcgctcgc gacggcgagc agctggacgc ggtccatcag cccgggccgt
    22381 cgggccggct cgcgcgcggt catcgcgtag aggacgagtc ccagcacgac cagtaggagc
    22441 gcgtcgaaga cgccgagcag ctcgcggt
//
LOCUS       DATABV010000073         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1950817, whole genome shotgun sequence.
ACCESSION   DATABV010000073 DATABV010000000
VERSION     DATABV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950817"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VHF88_02970"
     CDS             <1..1022
                     /locus_tag="VHF88_02970"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2160760.1"
                     /translation="REKIALFVNLPIDSVVSARDVDSIYKVPLYFRAEGVDDQILDRF
                     GIATEKPDLSAWESIVKRYEEATDVVKIGLVGKYTKLEDAYLSVIEALGHGGAHHGGK
                     VEVCWIDSEGLTDDEELAQLEGCDGILIPGGFGVRGIEGKIRAARWARESGVPYLGIC
                     LGMQIAVADFARHVVGLDGANSTEFDPETPFPVVDLLPEQKEVRDMGGTMRLGADPVK
                     LHDGTRAREIYGEAVIYERHRHRYEVNNHLRRRLEAEGLVCSGTSPDDRLVEIVELPD
                     HPFFVASQFHPEFKSRPLRPQPLFREFVGAALERARTKAGDGELAEAEIRASATARSD
                     AESRV"
     gene            1030..2235
                     /locus_tag="VHF88_02975"
     CDS             1030..2235
                     /locus_tag="VHF88_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2160761.1"
                     /translation="MTGAEGPRPSAPGPLRASSAEREQLAADFVRLCEIESPSRRERA
                     VAEAVAAELHSCGLRVEEDESAAATGSDSGNLLARIPGPPGAPTVLLCAHLDTVPLTA
                     PVEVLRESGRFSNRNDGILGADNKTAVAVLLAIARRYGPAGPSVPVELSALPGPPVGL
                     ELLFTTCEEIALLGAKELTHELRADFGFVFDHATPLGELIVAAPTYYRVDAEIHGAAA
                     HAGIRPEAGHNAIAVAAEAIASLSLGRLDDETTANVGTIHGGTAANVVAERCTVTLEA
                     RSLDDAKAGQAVGDIVDAFAEAAADMECDVETTVERLFRAYRLPAGSPPVRAASAALE
                     ALGIEPRPIATGGGSDANVFVADGLPVLNVANGTEGNHQPDESVTVAALETALDLTLG
                     ILGQSGSLA"
     gene            2250..>3002
                     /gene="ispG"
                     /locus_tag="VHF88_02980"
     CDS             2250..>3002
                     /gene="ispG"
                     /locus_tag="VHF88_02980"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEX2160762.1"
                     /translation="MPSKRQIDVGGVKIGGGAPVAVQSMTKTETADYDATMRQIRAIA
                     DAGGDLVRCAVPREKDVDALKRIVVESPIPVIADIHFNHTLALKAIEAGAHCVRLNPG
                     NIGGPDKVAEVVATAKRHGTPMRIGVNSGSLPKHLHELERENSVEALVQAAVEFVELM
                     ERLDFEDFKVSIKSTSVPNTIASNRLLSQKIDYPLHLGITEAGTKWSGSLKSAVGLGT
                     LLADGVGDTIRISLSTFHAEEEVKVGWEILKAL"
BASE COUNT          470 a         1114 c         1013 g          405 t
ORIGIN      
        1 tccgcgagaa gatcgcgctg ttcgtcaacc tgccgatcga ctccgtcgtc tcggcgcgcg
       61 acgtcgactc gatctacaag gtgccgctct acttccgcgc cgagggcgtc gacgaccaga
      121 tcctcgaccg cttcggcatc gccaccgaga agcccgacct gtcggcatgg gagtcgatcg
      181 tcaagcgcta cgaggaggcc accgacgtcg tcaagatcgg cctcgttggc aagtacacca
      241 agctcgagga cgcctacctc tcggtgatcg aggcactcgg ccacggcggc gcgcaccacg
      301 gcggcaaggt cgaggtctgc tggatcgact cggaggggct gaccgacgac gaggagctcg
      361 cgcagctcga gggctgcgac ggcatcctga tccccggcgg cttcggcgtg cgcgggatcg
      421 agggcaagat ccgcgccgcc cgctgggcgc gcgagagcgg cgtcccatat ctcggcatct
      481 gcctcgggat gcagatcgcg gtcgcagact ttgcgcgtca cgtcgtcggc ctcgacggag
      541 cgaactcgac cgagttcgat cccgagacgc cgttcccagt cgtcgacctg ctgcccgagc
      601 agaaggaggt ccgcgacatg ggcggcacga tgcggctcgg cgccgacccg gtgaagctgc
      661 acgacggcac ccgcgcgcgc gagatctacg gcgaggccgt catctacgag cgccaccgcc
      721 accgctacga ggtcaacaac cacctccggc gccggctcga ggccgagggc ctcgtctgct
      781 cgggcacctc gcccgatgac cggctcgtcg agatcgtcga gctgccggac cacccgttct
      841 tcgtcgcgtc gcagttccac ccggagttca agtcgcgtcc gctgcgcccg cagccgctgt
      901 tccgcgagtt cgtcggcgcc gcgctcgagc gggctcggac gaaggcgggc gacggcgagc
      961 tggcggaggc cgagatccgc gcctccgcca ctgcacgcag cgacgccgag tcgcgcgtct
     1021 agacgcgcgg tgaccggcgc ggaaggtccg aggccgagcg ctccgggacc gctacgcgcc
     1081 tcctccgccg agcgcgagca gctggccgcc gacttcgtgc ggctctgcga gatcgagagc
     1141 ccctcgcgcc gcgagcgcgc cgtagccgag gcggtcgccg cggaactcca cagctgcgga
     1201 ctgcgtgtgg aagaggacga gagcgccgcg gcaaccggtt cggacagcgg caacctgctc
     1261 gcccggatcc ccggtccacc cggggcgccg accgtgctgc tctgcgccca tctcgacacc
     1321 gtcccgctga ccgcgccggt cgaggtgctg cgcgagtccg ggcgcttctc caatcgcaac
     1381 gacgggattc tcggggccga caacaagacc gcggtcgccg tcctgctcgc gatcgcccgc
     1441 cgctacggtc ccgccggacc gtcggtcccc gtcgagttga gcgcgctgcc cggcccgccg
     1501 gtcggcctcg agctcctgtt caccacctgc gaggagatag ccctgctcgg cgccaaggag
     1561 ctcacccacg agctgcgggc cgacttcggc tttgtcttcg accacgcgac gccgctcggc
     1621 gagctgatcg tcgccgctcc gacctactac cgggtcgacg ctgaaatcca cggcgccgcc
     1681 gcgcacgcgg gcatccgtcc cgaggccggc cacaacgcga tcgccgtcgc cgccgaggcg
     1741 atcgcctcgc tctcgctcgg ccggctcgac gacgagacga cagccaacgt cggcacgatc
     1801 cacggcggga ccgccgccaa cgtcgtcgcc gagcgctgca cggtcacgct cgaggcgcgc
     1861 agcctcgacg acgccaaggc cggccaggcg gtcggcgaca tcgtcgacgc cttcgccgaa
     1921 gccgccgccg acatggagtg cgacgtcgag acgaccgtcg agcgactctt ccgcgcctac
     1981 cggctgcccg ccggctcgcc gccggtgcgc gctgcaagcg cagcgctcga ggcactcggc
     2041 atcgagccca ggccgatcgc gaccggcggc ggcagcgacg ccaacgtctt cgtcgccgac
     2101 ggcctgccgg tgctcaacgt cgccaacggc accgagggca accatcagcc ggacgagtcg
     2161 gtcaccgtcg cggcgctcga gacggccctc gacctgacgc tgggcatcct cggccagtcc
     2221 ggcagcctcg cctaggtacc cttggtccaa tgccctccaa gcgccagata gacgtcggcg
     2281 gcgtcaagat cggcggcggc gcaccggtcg cggtgcagtc gatgaccaag accgagaccg
     2341 ccgactacga cgcgacgatg cgccagatcc gcgcgatcgc ggacgccggc ggcgacctcg
     2401 tccgctgcgc ggtaccgcgc gagaaggacg tcgacgcgct gaagcggatc gtcgtcgagt
     2461 cgccgatccc ggtgatcgcc gacatccact tcaaccacac gctggcgctc aaggcgatcg
     2521 aggcgggcgc gcactgcgtt cggctcaacc ccggcaacat cggcggtccg gacaaggtcg
     2581 ccgaggttgt cgcgacggcg aagcggcacg gcacgccgat gcggatcggc gtcaactccg
     2641 gctcgctgcc caagcacctg cacgagctcg agcgcgagaa ctcggtcgag gccctcgtcc
     2701 aggcggccgt cgagttcgtc gagctgatgg agcggctcga cttcgaggac ttcaaggtct
     2761 cgatcaagtc gaccagcgtg ccgaacacga tcgcctcgaa ccgcctgctg tcgcagaaga
     2821 tcgactaccc gctgcacctc ggcatcaccg aggccgggac caagtggtcg ggctcgctga
     2881 agtcggccgt cggcctcggc acgctgctcg ccgacggggt gggggacacg atccggatct
     2941 cgctctcgac cttccacgcc gaggaggagg tcaaggtcgg ctgggagatc ctcaaggcgc
     3001 tc
//
LOCUS       DATABV010000074        30811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_263805, whole genome shotgun sequence.
ACCESSION   DATABV010000074 DATABV010000000
VERSION     DATABV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 30811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30811
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263805"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VHF88_02985"
     CDS             complement(<1..1802)
                     /locus_tag="VHF88_02985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX2160763.1"
                     /translation="MARITLADHPFEGLGGGLAVAAGALGLFCAWILLSTAWSDAPAR
                     GLIDFDRALLYWLALVLLGSVRWDGPRVAWAVRGVAVAIVGVCAVALLTRLFPDAIDV
                     AAAVAKDRLSYPLTYWNALGLMAAIGIVLCLHFTASEREPLAVRIIGAGALPLLAATL
                     YFTFSRGAAGVAVIGILVYVVVARPRSLVLGLAAAVPAIALTLVAAYDADLLASDDPT
                     TAAAAVQGGDVAGVVALCILGAAVLRALMAPADVRLRSLPVSAATRRKAWVATTTALV
                     AAALVSVVALDAPAKVADRVEGFTEQRDPSDEDDLRGRLTEASNNGRIKQWRVALDGF
                     GDEPLTGGGAGTFAHLWALERPSDLKVEDGHSLYLETLAELGLVGGLLLAVMLVVVIG
                     GFAARARGEYRHLYAALLGAGLAWAVHAGIDWDWEMPAVTLWLFALGGMALSVQRAGA
                     RFWAPPRFARVLLGVACLALIVPAGAMALSQWQLNRSVERLKTGDCSGAIDAALAAGR
                     AVPVRPEPFEVLGFCDVRLGKAELGVEMLEAAVEREPGNWMFHYGLALVRGAAGRDPR
                     PAARRALELNPREELARDAVRRFRTDDPREWRRGG"
     gene            2401..3762
                     /locus_tag="VHF88_02990"
     CDS             2401..3762
                     /locus_tag="VHF88_02990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2160764.1"
                     /translation="MGADAACGFAGGLVAATLIGLTLPGVELADVLVLALTSAICWPA
                     LSFIVGLYADEDLRSWVSGVSQAPRTLVTTILFAWPLFAVAHQIAMPHPGITTVAGTV
                     LTALFSGLARATVRGVLHRSPALRQRVLIVGSGVVAGQLAMKLRTHVQFGLVPIGVLD
                     DDLHPVGIAGLPWLGSLADLRHVIEEQEIDRVIIAFTRASHHDLLECIRASQEKGIAV
                     DVVPRLFELLDGGRALDRVGGLPLLSIGPARLGRASQIAKRALDIAVSSIALVLLTPL
                     IAALAVAIRVESKGPVLFRQRRVGRNGREFWLLKMRSMYHDADERKAELTEQNDLDDG
                     VMFKIHRDPRVTRIGRFLRRFSLDELPQLVNVLKGDMSLVGPRPLILPENEALTEAWH
                     HRRLELRPGLTGAWQIYGRSENPFDEMVRFDYQYVAGWSLARDIEILLATLPAVLSGR
                     GAY"
     gene            3795..4913
                     /gene="wecB"
                     /locus_tag="VHF88_02995"
     CDS             3795..4913
                     /gene="wecB"
                     /locus_tag="VHF88_02995"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX2160765.1"
                     /translation="MAEGAGIVHVVGARPNFVKMAPVIEALARRDDRPQLVVHTGQHY
                     DERMSGEMFSDLELPTPDLFLGIGSGTHGAQTARALADFEQVLLERRPELVVVGGDVN
                     STLACALAAAKLGIPVAHVESGLRSGDWTMPEEINRVLTDRLSDFLFTHSPEAEGNLL
                     SEGIASERIHPVGNTMIDSLRKFESKARDRRAWQPLGLREHEYVLITLHRPSNVDDPA
                     RLDLLVDQLCGLALRTPAVFPIHPRTRAQLEATGRLDELEAAGMRCIEPVGYLDFLSL
                     QADAGAVLTDSGGVQEETSALGVRCYTLRPNTERPVTIELGTNVLLGDDPAAIERVEL
                     GATDRAPCRIPLWDGQAGERIAEVLNPVPHAIAGVGGS"
     gene            5001..6590
                     /locus_tag="VHF88_03000"
     CDS             5001..6590
                     /locus_tag="VHF88_03000"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX2160766.1"
                     /translation="MLGDVANGPQRAVQIIRRNLGLILVCLLVVPGTALVVSLLQSKE
                     YEASASLLFRDPGLDQKLFGSSFFEPTDDPARVAATNVRLVSLDAVAARTARAMEVPG
                     LTSEDVSDNIEVEALGESDLIEVTAKNGDRQVAADLANTFASEYIAFRRDADRAKVRE
                     AQALVQSKLDALTPEEQAGVEGQGLASSARELEILASLQTGNAELVQPAAPPTSAASP
                     KVLRNVALALLLAIALAGGLVLLREQLDRRLKDPTDVRRVIDLPVLAAIPATRGHQGL
                     SSDLAAEGAIAEAFLMLRANLRYFNVDDDIRSILVTSAAPQEGKTTVSLNLARAEAAA
                     GKRVLLIEADLRRPTIARELGIGATTGGLTRVLTGQLSPAKAISSKLGIHVIIAGGLP
                     PNPAELLESERMRKVIQWGEEHYDLVVVDTPPTAVVADAIPLVTAVNGVVVVVRLGQS
                     RRDALQQLRDQLAHIEAPILGVVLNDSAQPRRSDYYGRPSGMMTSTTKPARTERTPNG
                     STASPSKTGKAAAQGQPTRKA"
     gene            6823..8079
                     /locus_tag="VHF88_03005"
     CDS             6823..8079
                     /locus_tag="VHF88_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HEX2160767.1"
                     /translation="MPSAERFGYAVTEMERYDVAPAPRTGAYVLSSARSRLTLRRVHV
                     RTRATAAAVSRLLTKRARDGSSRRVLAARRAGRDAVTAVVLTVPRSAPRPTKRQLARL
                     RRLARSLRGGSARRLSTRDRDSRAPKPGRGKPPRTEPVEPPTTEPLEPPTTEPAEPPA
                     SEPVEPPTPSEPVEPPTTPSEPAGPPTTPPDSLASGQLLFEDRFDSGSAGEGIWGPKQ
                     AAPGSLDYVESPVFPGRSKAMRNRLEPGDLHAGTNSSRSELTGSLKFRDGDVRWFRMP
                     VRFNEWSLQSGAWALIWQAHSGSGSPPLAAYVQSDELLFRVKNGYTGQIFWEQKFELH
                     RWYDLVIGTQFSHGGDGWVEVWVDGVRQKLRNGQQRMTGATLSPTSSYSYDKLGLYQA
                     SSFSSGARELLHGMYKVGTTRESVVR"
     gene            complement(8085..9407)
                     /locus_tag="VHF88_03010"
     CDS             complement(8085..9407)
                     /locus_tag="VHF88_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX2160768.1"
                     /translation="MRRPVTLRPIFTPGDGTVPGPRGAAGLGWALAALAAAGTGALAA
                     RAPALAVGMLALLGAAIVVVTASRLVLVQLLLGALPWLLVLDAFVPPLVRTAATGIAA
                     VALVAFALPLRYQRALGPIGAGVFAVVVLAHAVVATDVEQLKQAASYMIFPVVALAVL
                     SERGRNELPRVRNAVFASGFAAMVVHLLVAASGVGAGDTKYGVGEKLGFAAEAPHEMA
                     LLSVVIAAAALTMSERVSVRAGLFTVAALPALLTGVRSAMLSVALILLVMLIESRADR
                     RSVAVVGIALALAIAGGAGAVLTERLSETFRSGATAETASSSRTAIWGVALDLWADSG
                     PPGWVLGAGLRSVPEAELFELGVPYIGHSDIIEVGVQIGGLGLLAWLLVWAALFRAGL
                     RTMLLIPVVVYAVVNGAIGYVAPLTLALVLAAACRRDADRTPRLARDG"
     gene            9504..10625
                     /locus_tag="VHF88_03015"
     CDS             9504..10625
                     /locus_tag="VHF88_03015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2160769.1"
                     /translation="MGDQRTTTPRRDRRRGRVLHVTPDPQRPGGMAAVVRDLLASPLG
                     DRYALDVLVSHRTRDRLGRIAVFVRALWRLSRWCRGAGSRIVHVHSAVRGSMYRKALC
                     VAVAKSAGRPVVLQLHAGAGDIEAFDASLGRLRRALVGHALRRADRVVSVSAPGAERI
                     ERCFGVHGVVVIPNAAPAIRAARGARDPDRPVRALYMGGFVNPVKGGAVLIEALPRLL
                     AGDPNLEVAFAGTGTPPDELLEAARRHEGVSWLGWLDAEAKAEQLERCDVFVMPSLSE
                     GMPIALLEAMAYGCAIVASAVGGVPEVVADGSEAVLVPPSDPAALADAVARTAADPAL
                     RARLGAAAHERIGDFDAGNVYDRFAALYDELLALPGATR"
     gene            10622..11437
                     /locus_tag="VHF88_03020"
     CDS             10622..11437
                     /locus_tag="VHF88_03020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX2160770.1"
                     /translation="MSAGRLRNRSREAVFLGYHSVAEEGPPFLSVTPEAFERHLALLK
                     RRGFRSGTLADLAHVAGGGRPDGKLAFLTFDDGYVDNYATARPLLEAYGFRAIVFLVP
                     PLIDGGGPLVWERVERHVRDYPAVMRAMTWTMVESMAAEGHEFGSHTLTHPRLPDLDD
                     ERLRRELSESREQVERRLGSCEALAYPFGLWSPRVAAATGRAGYSYAFTLPYGAQTHA
                     DRLTIPRIAIDHRDDERRFGLKLTRAYRAFALSRGKPALRRLLRRAPAHQSPA"
     gene            complement(11463..12221)
                     /locus_tag="VHF88_03025"
     CDS             complement(11463..12221)
                     /locus_tag="VHF88_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX2160771.1"
                     /translation="MPGTTSDIDAPPLLGLPCAVIDVEGAREFAVRAVEGGEFNSVLF
                     ANAAKVVMAESDAELRAALFDATLLAADGQSLVWASRILGQPLPERVAGIDYMLDLLQ
                     VADSRGWRVFFLGGRDEVLEAVEDYCRRRHPGLIVAGRHNGYFGPERDAEVAAIVRKS
                     DADVMFVGMPSPRKELWIDRQGPATGVSLAVASGGSFDVIAGVVRRAPALWQRLGLEW
                     FWRVVQEPRRLWKRYLTTNSRFLAKVARERLRGR"
     gene            complement(12255..13352)
                     /locus_tag="VHF88_03030"
     CDS             complement(12255..13352)
                     /locus_tag="VHF88_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160772.1"
                     /translation="MAAEGLAFDSRAAELLEPAMAALPAMVVPSGLVCYEVAEGDSAP
                     RGESVRYALIVLLGLLRARHAGAAPSIDPDKLRDAIDAALERPEVTPGDVALRLWVES
                     RAETGSANTLVERLDRSLDVNGGLAAREGLELGWIVTALAVTEDLTARGRELRKAALD
                     QLIASNQGPSGLFRHFGEGGARRRFPNFATQIYSVLALAVTAGRGLDARALPAARRAA
                     DRLLALQLPDGGWPWMYDAERGSVVERYEVYSVHQHGMAPMTLYELSGVSGEERYAAA
                     GDRGIPWLFGANELGAQMAPLERGMTYRSIRRVRPLDRLCLYANTATAAAIGRSRAGR
                     GRRLELNRTCRPYELGWLVEAWAAMNGAAQR"
     gene            complement(13342..14277)
                     /locus_tag="VHF88_03035"
     CDS             complement(13342..14277)
                     /locus_tag="VHF88_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX2160773.1"
                     /translation="MSRGALRALVLRRAPAPALRALRRAQRSRARMRHRVRRRLDPVR
                     VTGAEIGAALAAAGLSRGDNVFFQSAMSPFGEIEGGPETVLRALDEVLGAEALVAMPA
                     FPFRGSVLEYLRDDPCFSVRSTPSSMGSVSERFRQLPGTVRSLHPTHSVSARGPGAES
                     LVAGHELALTPFGEGTPFARLLERDAYQVWFGTDVHAFTLYHTFECLLGDRFPIDVFL
                     EKPVSVRCEDAAGEPLRVATLVHDPAVAAHRIRSQARQQVRAELLAEGVMRAVPLGAS
                     ELLVCRIPAMFTVLDRLLAQGLTIYDIDLEGRDGR"
     gene            14336..15793
                     /locus_tag="VHF88_03040"
     CDS             14336..15793
                     /locus_tag="VHF88_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HEX2160774.1"
                     /translation="MTLFSGAVAVAVVALVLAVGASEPAGALVLKHRPTGFTVHAPPD
                     FRVQFDKRSTRYTVRSRNRRARLTYTRLRTKRSPAAIARSRAARVARSQLRVVRAGRR
                     YRLRVSRRRVVEVGRAGRGTVAVSTLSTRGRRTAARLALLRRIGRSARGGLARNTGTT
                     HGLSVAWRTPTAGQILAPGSAELRVDASTPSRVAEVRFLVDGEHLHTEYETPYTGVLD
                     TSGLSAGLHKLRAVVVLRDGRAIVRNQTVAVRGAGSAGETSPAAGGGCASNALFEGGF
                     EGGLDEWGNVQALGPRATIVQGGSFCGARHGRFEVRAGDVEPDTDSNRAEVAGPSFSD
                     GDDLWFRHATKLAAGFPREDKWQIVSQWRSGDGSPPLALFVSDDLALSLRRGDAPYTE
                     FWRGPVQTEVWTDLVFHIRFSRNPALGFVELWRDGRRQTMRNGAERMSGQTIKGSSGY
                     VKLGYYRDPDHVGTGVVYHDNYLVGRSLADVAPAP"
     gene            15839..17173
                     /locus_tag="VHF88_03045"
     CDS             15839..17173
                     /locus_tag="VHF88_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160775.1"
                     /translation="MPLQRLRGLSTFKRGFASTFTLDVLARAMSAMTLVLLLRALPVD
                     GFAFVILLLSIGQFMGSAATGGLRMRYVRIQAERVSRESDEPSSFYLTLRGGIVLIVA
                     AGALGFLGATALDVGTPEERAVMALIATAYTLGHASIELSAVHYQAHVAFVKAGVLIW
                     LRSFVQFLFAVGATAGLLKTGTAVGAAFAIGVMCVAVATAGPPAWATRKSTVDREGRF
                     GFGRESAALTLFSIATAGRAYINIFLVAALLDDTAVTVYGTATRYISVVMGPVPALMT
                     VMRVRTAQSDMVDSHETQLRLMVDWARRSLVPLIVALGAAAIAAPFVIPLIDEGKYPD
                     TVPVFEIMLVMAFAQLMTLPNPSLLLTQNRYSTLAWVDATVVVVNVLLALAVASHFGV
                     IGIVVVAALVTVGQVCAVTYLAAHPPKGTAGAPAAPLSEGLEEHPESAVRDP"
     gene            complement(17170..18258)
                     /locus_tag="VHF88_03050"
     CDS             complement(17170..18258)
                     /locus_tag="VHF88_03050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2160776.1"
                     /translation="MAGSPAATTVDWVADEAGFAALAEPWDALLTPDSHPFDLHCWYA
                     AWWKAFGGDRQLAVCVVRRGHQIVAVAPLMRDSSGRILAMANEQSSMFRLLAVDADAR
                     RALARALLERRAPELFLSRIPADEPIIGDLCELGRSSGRLVASEPEHVSPIVDTSGDF
                     DAWRADSKRRWGAPLERFRRKMQRDYDAEMTIVEPPVDLDGELAAGLRVEASGWKGQA
                     GTAILCDPATHAFYVEMSRAFHERGALRLSRIVLDGRTAAFDLCLLDRGRLYLVKTAF
                     DEDFRKLAPGLVLRLSTVERCFELGLEAHELLGHRSEWKAKFATRERPHVGVRAYPRR
                     PLGVARYAYRTSIRPPLRRAYRRVRARG"
     gene            complement(18258..19382)
                     /locus_tag="VHF88_03055"
     CDS             complement(18258..19382)
                     /locus_tag="VHF88_03055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2160777.1"
                     /translation="MPVAVEILDLESDLESFRSQWDALAVETGQPYSSPAWMLAWWRH
                     ARPADALLRAVVVVEGERLIGLAPLWVAGDRAGGTRYAMLASRLSPPVRPLAATGREN
                     EVAAAIASALARSTPRPHALQLEDESDSTQWAMSLADAWPGRRPWRYSPAAMAAPAIV
                     LGGLDYDAWLQGRNAKFRQETRRRRRQLDKAGGRFSLAGENDFDSALAAFLRLHRDRW
                     DYRGGSNALVDGIEPMLAEAGRELLPSERFRLFTIDVDGEVICAHVFIAAGSEVSGWN
                     GGFDASWSRYGPSILTMLEAVADAAQRGERRISLGPGDQPYKGTLADERSEISLTTLV
                     PRGAAYPLARARLAPYQARGELAKRLSPEAKRRVRGLVRR"
     regulatory      19487..19643
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            19700..20101
                     /locus_tag="VHF88_03060"
     CDS             19700..20101
                     /locus_tag="VHF88_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2160778.1"
                     /translation="MPSAAAAKQSDRESAAVMVDRINSTRAKHDLPSLRAAPRLMRSA
                     RRYAAHLMRADAFEHGPARITGFRASGEMLAFNPGWRLRTSPVVRMWLGSPGHRALML
                     SRSFGHVGVSPARGRFGGALTTIWVVQLGRR"
     gene            complement(20098..20601)
                     /locus_tag="VHF88_03065"
     CDS             complement(20098..20601)
                     /locus_tag="VHF88_03065"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX2160779.1"
                     /translation="MTVDTDQATREASGARPYGRYLEDFEVGAVYKHWPAKTVTEADD
                     HMFCLITMNHHPLHINDVYAKQSQQGRNVVVGPLVYSLALGMSVGDVSGKAIANLATD
                     ELSHPAPVFHGDTLFCESEVLEVRPSSSKPDRGVVRVHTRVLNQDGVLVAEFKRSVLV
                     PRREPGA"
     gene            20677..21753
                     /locus_tag="VHF88_03070"
     CDS             20677..21753
                     /locus_tag="VHF88_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX2160780.1"
                     /translation="MRNPRDFFKPLAVGAPDPVAEVPFKPSRMIHFLDFSNEKMVAKA
                     PAIAGQVDILLGNLEDAIPIDRKEAAREGLITVGRDMELGDTALWVRVNSLESPWVLD
                     DLTELVATIGDKLETIMVPKVEGAWDIHYVDRLLAQLEAKAGVEKPIMVHAILETGMG
                     VVNLEEIATASPRMQGMSFGPADLAASRRMKTTRVGGGHPGYRVIEDPDPDQPDAPRI
                     TAQQDPWHYSIARMVDACTSAGILPFYGPYGDIKDVDGCEAQFRAAFLLGCVGAWSLH
                     PVQIDIAKKVFSPDPNEVAFAKKVIEAIPDGAGVHMIDGKMQDDATWKQCKVMVNLAE
                     MLAKKDPELAEAYGFERATAGAAS"
     gene            21750..23012
                     /locus_tag="VHF88_03075"
     CDS             21750..23012
                     /locus_tag="VHF88_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX2160781.1"
                     /translation="MKFYEYEARQIVEQAGIPVTKYGFAKTAEEARAIAADIDGPTVI
                     KSQVLTGGRMKAGGVKFADTPDEAAAHAEDILKLEINGHMPRGVLVDPKAQVKQEYYA
                     GVVWDGTRKQPLLLFSDMGGIDIEQVAEEHPDHVGRGHFSNLLPYSDFQAKQVIAAAG
                     VSGKELTRLTPILTRLARLFQTCDMTLAEINPLAQLEDGSFVALDAHMEMENEAMGRQ
                     KKLLESIGIGAEEGTREGHEPSPFEAIVKQIDSADHRGVIQGKDTGFHGNVGLVIGAG
                     GGSLTLTDAVRSQGGKPANYSEIGGNPSVAKACGLAKAVLQKEGVEKIAVMMSIVSNT
                     RVDIVARGVIKACLELGKDPGETVAIFRIPGAWEDEGFKILDRYGIEYCDRSVSMHEA
                     ARRAVEKIQGEGGPKQPALPDAQGKVSA"
     gene            23009..23950
                     /locus_tag="VHF88_03080"
     CDS             23009..23950
                     /locus_tag="VHF88_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2160782.1"
                     /translation="MSILIDENTTFIVQGITGREAVNLTRECLDYGSKIVGGVTPGRK
                     GRDVHGVPVFDTVEQVIAHNDGRVPDGSVVTVPPAFTKDAVFEAIENGIKLIVIVTER
                     IPRRDVAQMVELANLRGARIIGPNCLGLIVPGVAKMGGIGGPAKNAAQAYQPGSIGVM
                     SRSGGMTTEISSTLTAAGLGVSTAISIGGDAIIGSAYAELMPLFEADEQTEAIVIYTE
                     PGGRMEAQLAEWVAEHDSRLPIVAFMAGRFMDDEEMQGMSFGHAGTIVEGKEDTATEK
                     IARLESAGIPVVERIDEIPDAVKRKLADRKSEKGAVA"
     gene            23950..24186
                     /locus_tag="VHF88_03085"
     CDS             23950..24186
                     /locus_tag="VHF88_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160783.1"
                     /translation="MSRIFIDIEVADEVRDDTELARKLAEVCPVDIYAERDGKVAVVD
                     ENVDECVLCGLCLDASPPGAVAVKKLYDGTTLTR"
     gene            24236..25510
                     /locus_tag="VHF88_03090"
     CDS             24236..25510
                     /locus_tag="VHF88_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX2160784.1"
                     /translation="MTSPNGKPFSRVGVIGLGYVGLPLAVAFAEEGVDVVGVDYAPQL
                     VAAIEAGESHIEDIASERLQAVGDRIAATTDYGRLADCDAVLIAVPTPLTRNREPDLG
                     PLLSSAQALSGVVRTGQLVVLESTTYPGTTREQLVPLLEESGLAAGRDFNVAFSPERV
                     DPGRTDHTLRTTPKIVGGLTPACLARAVALYEIVCDNLVEVSAPEPAELAKLLENVFR
                     SVNIALVNELAMLCDRMGIDVWEVIDAAATKPYGFMRFDPGPGMGGHCLPVDPFYLSW
                     KAREYGQPTEFIELAGEVNQRMPEFCVDRIARALNDHSKPVRGSKIAIVGVSYKSGVG
                     DLRESPALRIMGLLAEGGAVLAYHDDFIAEIPELGYSGETLDAALDGADAAVIVTAHP
                     GLDLERIVAAAPLVVDFRGVTRGIEAPNLVRL"
     gene            complement(25518..26009)
                     /locus_tag="VHF88_03095"
     CDS             complement(25518..26009)
                     /locus_tag="VHF88_03095"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HEX2160785.1"
                     /translation="MKPVLFVDVDGVLSLFGFDPGSGLPGPFHWIDGIAHCIPPDSGP
                     RLARLADRFELVWATGWEEKANEYLPRILGLPFHELPCLTFDGRARFGSSHWKVEAID
                     EYARGRPAAWIDDNVDDECLAWARGRSEPTLLIETQPAVGITDEHVAELLAWAADVAP
                     AVG"
     gene            complement(26012..26368)
                     /locus_tag="VHF88_03100"
     CDS             complement(26012..26368)
                     /locus_tag="VHF88_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160786.1"
                     /translation="MSATDTKALIADAIEQLTTEVPSLRQLKLVVRLELPGRGSDAPI
                     WRVEVPGPKVERDPAGDARLSVVVQRQQFNELATEGTLRQWAKAYEQGHVKVTGDSAV
                     VKLLGNVMERQLVRAK"
     gene            complement(26411..27271)
                     /locus_tag="VHF88_03105"
     CDS             complement(26411..27271)
                     /locus_tag="VHF88_03105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2160787.1"
                     /translation="MEVRAGALLAPVNWHSPMFGPLAVDRSVQEELMERVFAEPSARV
                     EMNLLCDSQAPLETLVAAARRQRRVVLSRAVTRSPIIDTTGCVTEYEQTLSRNRRRAL
                     RRQRRRLEQLGELTFEVHDGSERLDELLADLYRVEASGWKGDRGTAIQSQPETKRFYT
                     AVARWAAEGGWLRLACLRLEGRAIACDYVIEHGGTWYSLKAGYDEEFRSFGPGALLLR
                     EEIGHCLASGVTCLDLLGNEDAFKSSWADRSVERSWMGAFRRTPRGLAAWLRKAGREA
                     ARRSLRAFKK"
     gene            complement(27516..29813)
                     /gene="pabB"
                     /locus_tag="VHF88_03110"
     CDS             complement(27516..29813)
                     /gene="pabB"
                     /locus_tag="VHF88_03110"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX2160788.1"
                     /translation="MVESGLSAVTACEDRRLRVTTMKTLLIDNYDSFTYNLFQLLAEV
                     NGELPEVVCNDAADWEELARRDFDNVVISPGPGRADDPADFGVCGEAVRRAEVPLLGV
                     CLGHQGICSALGGRVEHAPEAVHGRAGAVIHDGSALFVGIPREFQAVRYHSLCAGWAL
                     PDELEPTAWASDGVLMAVAHRTRPLWGVQFHPESIATQHGRRLLANFRDLTAAAARPR
                     RADSGGNVAETHPTSAPSPRRVDLGGDVAEQGSVSGAPVESNDSGERASPSDPLSLVV
                     ERLDMLADPEAAFAALYADSADAFWLDSARVEGEARFSFAGDASGPFGAVVTYDVDAG
                     EVTVTAAGGVERHRETIFDYLRREQARLREVAAPPDLPFDFACGFAGYLGYELKADCG
                     GDAVYDSPLPDAAFVRADRMLAFDHEEGHTYLLCLTDVVNTHDAERWIADTRQRLESL
                     PPPGDLTSRSGPQGPAPAHERPRAAAPPGEFSLARSRERYLADIAVCKERLFDGETYE
                     VCLTNQIATDFDGDPLVLYRELRRVNPAPYAAFLRFGGVAVLSSSPERFLRVGRDRWA
                     EAKPIKGTARRGLTPVDDVRLAEELRTSEKDRAENLMITDLLRNDLGRVCEVGTVHVP
                     HLMQVETYETVHQLVSTVRGLLQPETDTADCIRACFPPGSMTGAPKKRTMEIVDELEE
                     APRGVYSGAIGYLGPGGSCDLSVTIRTIVVADGKATIGVGGAIVTQSNADDEFHEALL
                     KGEAPMRAVRGVGEPLELAPICAHW"
     gene            30011..30154
                     /locus_tag="VHF88_03115"
     CDS             30011..30154
                     /locus_tag="VHF88_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160789.1"
                     /translation="MGVELDGATVDDEQRLEDAARGVGAEEICRRERAADRHGLRGSH
                     AER"
     gene            30141..>30811
                     /locus_tag="VHF88_03120"
     CDS             30141..>30811
                     /locus_tag="VHF88_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX2160790.1"
                     /translation="MLSGETMKTLLIDNYDSFTHNLFQLLGEVNGEQPEVVRNDEASW
                     DELARLPFDNVVISPGPGRPDDPQDFGVCADAIRRAEVPLLGVCLGHQGICAAYGGHV
                     ERAAEAVHGRTSAVLHDGSPLFAGIPREFQAVRYHSLAAGWELPEELEPTAWTSDGVL
                     MAVAHRSRPLWGVQFHPESIATEHGRRLLANFRDLTPARSLEARGKPSPSPSRRVASC
                     GRNTSA"
BASE COUNT         4659 a        11183 c        10723 g         4246 t
ORIGIN      
        1 ccccccctcc tccactctcg ggggtcgtcc gttcgaaagc gccggacggc gtcgcgggcc
       61 agctcctcgc gcgggttcag ctcgagcgca cgccgcgccg ccggacgcgg gtcgcggccg
      121 gcggctcccc ggaccagcgc aagtccgtag tggaacatcc agttgccggg ctcgcgctcg
      181 accgcggcct ctagcatctc cacgcccagc tcggccttcc cgagccgcac gtcgcagaag
      241 ccgagcacct cgaacggctc aggccggacc ggcaccgccc tgccggctgc cagcgccgca
      301 tcgatcgctc cgctgcagtc tccggtcttg agccgctcca cgctgcggtt caactgccac
      361 tgcgacagag ccatggcgcc ggccgggacg atcagcgcga gacaggcgac tccgagcagg
      421 acccgtgcga agcgcggcgg agcccagaac cgcgcgccgg cgcgctggac cgacagcgcc
      481 atgccgccga gcgcgaacag ccagagggtg accgcaggca tctcccagtc ccagtcgatg
      541 ccggcatgca ccgcccatgc cagcccggcc ccgagcagcg ccgcatatag gtgtcggtac
      601 tccccacggg cgcgcgcggc gaagccaccg atcacgacga cgagcatgac ggcgagcaac
      661 agtcccccga ccagccccag ctcggccagc gtctcgagat acagcgaatg gccgtcctcg
      721 accttgaggt cagagggccg ctccaacgcc cacaggtggg cgaacgtgcc cgcgccgcct
      781 cccgtcagcg gctcgtcgcc gaagccgtcc agcgccaccc gccactgctt gatccggccg
      841 ttgttgctgg cctcggtgag ccggccgcgc aggtcgtcct cgtcggaggg gtcccgctgc
      901 tcggtgaagc cctcgactcg atccgcaacc ttcgccggcg cgtcgagggc gacgacgctg
      961 acgagcgcag ccgccacgag cgcggtggtc gtggccaccc acgccttgcg ccgcgtggcc
     1021 gcggacaccg gcagcgagcg caggcgcacg tcggccggcg ccatgagggc acgcaggaca
     1081 gccgcgccga ggatgcagag ggccacgacg cccgcgacat cgcctccctg gaccgcggcg
     1141 gccgctgtgg tcgggtcgtc gctcgccagc agatcggcgt cgtaggcggc cacgagcgtc
     1201 agggcgatgg cgggcaccgc agcggccagt cctagaacga gcgaccgggg ccgcgcgacg
     1261 accacataga ccaggatccc gatgaccgcc acgccagccg cgccgcggga gaacgtgaag
     1321 tagagcgtcg cggcaagcag cggaagcgcc ccggcgccga tgattcgcac ggctaggggc
     1381 tcgcgctcgc tggccgtgaa gtgcaagcac agcacgatgc cgatcgccgc catcagcccg
     1441 agcgcgttcc agtaggtgag cggatagctc aagcggtcct tggcaaccgc cgccgccacg
     1501 tcgatcgcat ccgggaagag ccgtgtcagc aacgcgacgg cgcagacgcc cacgatcgcc
     1561 acggcaacac cccggactgc ccacgcgacg cgcggcccgt cccagcggac cgagcccagc
     1621 agcacgagcg ccagccagta gagcagggcg cggtcgaaat cgatcagccc gcgtgccggt
     1681 gcgtccgacc aggccgtcga gagcaggatc catgcgcaga agagccctag cgcgccggcg
     1741 gcgacggcca agccgccgcc gagcccctcg aacggatgat cggcgagggt gatcctcgcc
     1801 acgagcaaga ggcccagcac cactgccagg agcgcagggg tcccggcgaa gtatccaccg
     1861 gcctggaacg acgcgtaggc ggtcagtgcg cccggcaggg cgatcaggac ggcgattccg
     1921 agccggctgg cgcgactggc cctaccagcc ggggtggcgg tatcggatcc accacctcca
     1981 tcagggagac cggaagagat cttggcgatt tcgcccaacg agtcctatgt tctattcgaa
     2041 agcatggttg ggcctctgcg gaggtctcag ggcgctggag ggtacggagc cagctccgct
     2101 caccgcccgt aagccggaaa cgataggaac cagatcacgc agctcccccc atagtcgccg
     2161 cgagagcgga tggatcgcct gagagatccc gcctaaagaa gacccgcttc gatgccgacc
     2221 taagccctag cccgattcca ttctgaactt ctaagacgtc aggacctgta gagcgtggag
     2281 agctcaaccg caacccaggc aaacaccagc gcgcacttca atcgccagcc gccggcgtcc
     2341 acgccggcac ccgcttcgca gcgcgctcgg cagcggtcgg gggtgctgag caggcgactg
     2401 ttgggtgcag acgcggcctg cggcttcgcc ggagggctgg tagccgccac gctgatcggt
     2461 ctgacgctgc ccggtgtgga gctcgccgac gtgctggtgc tcgcgctgac atccgcgatc
     2521 tgctggcccg cgctctcgtt catcgtcggg ctctacgcag acgaggacct ccgctcctgg
     2581 gtcagcgggg tcagccaggc gcctcgcacg ctcgtcacga cgatcctctt cgcgtggccc
     2641 ctcttcgccg tcgcacacca aatcgcgatg ccccacccgg gaatcacgac ggtcgccggc
     2701 accgtcctca ccgccctctt cagcggactg gcgcgcgcca cggtgcgcgg cgtccttcac
     2761 cgctcgccgg cgctgcgaca gcgtgtgctg atcgtgggct cgggcgtcgt ggccggccag
     2821 ctcgcgatga agctgcgcac ccacgtgcag ttcggcctcg tgccgatcgg cgtgctcgac
     2881 gacgacctgc acccggtcgg catcgccgga cttccgtggc tcggctcgct ggccgatctc
     2941 cggcatgtca tcgaggagca ggagatcgac cgggtgatca tcgcgttcac gcgcgccagc
     3001 caccacgact tgctcgaatg catccgagcg tcgcaggaga agggcatcgc ggtcgacgtg
     3061 gtgccgcggc tcttcgagct gctcgacggc ggacgcgcgc tcgatcgcgt cggcgggctg
     3121 ccgctgctgt cgatcggccc agctcgactc gggcgcgctt cgcagatcgc caagcgggcg
     3181 ctcgatatcg ccgtctcgtc gatcgcgctg gtgctcctga cgccgctcat cgcagcgctc
     3241 gccgtggcga tcagggtcga gtccaagggc cccgtgctgt tccgtcagcg ccgagtcggg
     3301 cgcaacggac gcgagttctg gctgctcaag atgcgatcga tgtaccacga cgccgacgag
     3361 cgcaaggccg agctgacaga gcagaacgac ctcgacgacg gcgtcatgtt caagatccac
     3421 cgcgacccgc gcgtgacccg gatcggccgg ttcctgcgcc gcttctcgct cgacgagcta
     3481 ccgcagctgg tcaacgtgct caagggcgac atgagcctgg tcgggccgcg tccgctgatc
     3541 ctgcccgaga acgaggcgct gaccgaagcg tggcaccacc ggcggctcga gctgcgaccc
     3601 ggtttgaccg gggcatggca gatctacggc cgctcggaga acccgttcga cgagatggtg
     3661 cgcttcgact accagtacgt ggccggctgg tcgctggccc gcgacatcga gatcctgctc
     3721 gcgacgctgc cggccgtcct ttcgggacgg ggggcgtact gagcccggcc gcccctcacg
     3781 aggaccccgc gacgatggcc gagggcgccg ggatcgtgca tgtcgtcggg gcgcgcccca
     3841 acttcgtgaa gatggcgccc gtcatcgagg cgctcgcccg tcgcgacgac cggccgcagc
     3901 tggtggtcca caccggacag cactacgacg agcggatgtc gggcgagatg ttcagcgacc
     3961 tcgagctccc gacgccggat ctcttcctcg gcatcggctc cgggacgcac ggcgcccaga
     4021 ctgcgcgcgc gctcgcggac ttcgagcagg tcctgctcga gcggcggccc gagcttgtcg
     4081 tcgtcggcgg cgacgtcaac tcgacgctcg cgtgcgcgct cgccgccgcc aagctcggga
     4141 tcccggtcgc acacgtcgag tccggcctgc gctccggcga ctggacgatg cccgaggaga
     4201 tcaaccgggt cctcaccgac cgactgtcgg acttcctgtt cacgcacagc cccgaggccg
     4261 agggcaacct gctgtccgag ggcatcgcga gcgagcgcat ccacccggtc ggcaacacga
     4321 tgatcgactc tttgcgcaag ttcgagagca aggcgcgcga ccgcagggcc tggcagccgc
     4381 ttgggctgcg cgagcacgag tacgtgctga tcacgctgca ccgcccgagc aacgtcgacg
     4441 acccggctcg cctcgacctg ctcgtcgatc agctctgcgg cctggccctg cgcacgccgg
     4501 ccgtcttccc gatccacccc aggacgcggg cccagctcga ggcgaccggc cgtctcgacg
     4561 agctcgaggc cgccggcatg cgctgcatcg agccggtcgg ctatctcgac ttcctgtcgc
     4621 tgcaggccga tgcaggcgcc gtgctgaccg actcgggagg cgttcaggag gagacgtcgg
     4681 cgctgggagt ccgctgctac acgctgcggc cgaacaccga gcggccggtg acgatcgagc
     4741 tgggtaccaa cgtcctgctc ggcgacgatc ccgcggcaat cgagagggtg gagcttggtg
     4801 cgaccgaccg agcgccgtgc cggattccgc tctgggatgg ccaagccggt gagcggatcg
     4861 cggaggtgct gaatcccgtg ccgcacgcga tcgctggtgt gggcggaagc tgacgccgct
     4921 tcttctgacc cgttcgtcac actgtgacgg actcgccgat gccaccgcca ccgtcaactc
     4981 agcccgctgc ggcctcgccg atgctcggag acgtagccaa cggtccgcag cgcgcggtgc
     5041 agatcatccg ccgcaacctc ggcttgatcc tcgtctgcct gctggtggtg ccggggacgg
     5101 cgctggtcgt atcgctgctg cagagcaagg agtacgaggc gtccgcgtcg ctgctgttcc
     5161 gcgatcccgg gctcgaccag aagctcttcg gatcctcgtt cttcgagccg accgacgatc
     5221 cggcccgcgt ggcggcgacc aacgtccgtc tggtgtcgct cgacgcggtc gccgcgcgta
     5281 cggcgcgcgc gatggaggta cccgggctca cgtccgagga cgtctcggac aacatcgagg
     5341 tggaggcgct cggggagtca gacctgatcg aggtgaccgc gaaaaacggc gaccggcagg
     5401 tcgccgccga cctcgcgaac accttcgcaa gcgagtacat cgccttccgc agggacgccg
     5461 accgcgccaa ggttcgtgag gcccaggcgc tcgtgcagag caagctcgac gcgctgacgc
     5521 cggaggagca ggcgggcgtc gagggtcagg gactcgcctc cagcgcgcgc gagctcgaga
     5581 tcctggcctc tctgcagacc ggcaacgccg agctggtcca gccggctgcg ccgccgacct
     5641 cggcggcctc tcccaaggtc ctgaggaacg tcgcgctggc cctgctgctc gccatcgcgc
     5701 tggcgggcgg gctggtgctg ctgcgcgagc agctcgatcg ccgcctcaag gatccgaccg
     5761 acgttcgccg cgtcatcgat ctgccggttc tggccgccat cccggcaacc cgcgggcacc
     5821 aggggctctc cagcgacctc gccgcagagg gcgcgatcgc ggaggcgttc ctgatgctgc
     5881 gcgccaacct ccgctacttc aacgtcgacg acgacatccg ctcgatcctt gtcacttcgg
     5941 cggcgcccca ggagggcaag accaccgtct cactgaacct cgcgcgggca gaggctgcag
     6001 ccggcaagcg cgtgctgctg atcgaggccg acctgcgccg accgacgatc gcccgcgagc
     6061 tggggatagg ggcgaccacg ggcgggctga cgcgtgtcct gaccggccag ctcagcccgg
     6121 cgaaggcgat ctcctcgaag ctcggaatcc acgtgatcat cgccggcgga ctgcctccga
     6181 atcccgccga gctgctcgag tccgagcgca tgcgcaaggt gatccagtgg ggcgaggagc
     6241 actacgacct ggtggtcgtc gacacgccgc cgacggccgt cgtcgccgac gcgatcccgc
     6301 tcgtcaccgc ggtcaacggc gttgtcgtcg tcgtgcggct cggacagagc cggcgcgacg
     6361 ccctccagca gctgcgcgac cagctcgcgc acatcgaggc gccgatcctc ggcgtcgttc
     6421 tcaatgacag cgcacagccg cggcgcagcg actactacgg caggccttcc ggcatgatga
     6481 cctcgacgac caagccggct cggacggagc gcacccccaa cgggagcacg gcctccccgt
     6541 ccaagaccgg caaggcagcg gctcagggcc agccgacccg caaggcctga cccagcgccg
     6601 gtatccgacg gcgccgccgc cgcttgctga aagccgcctt gcagcggcgc caacatatgt
     6661 ccaggccgcc ggctcgtcac gtgctggcgg gcgcaggctg ccgacagata gcccatgcgg
     6721 cactccggca tgggactcgg gcgccggtgc gccgctcacg cggccctcct cctggcggtc
     6781 gcgggatccc tggcgatcgc gccagccagc gcggccggcg cggtgccctc cgccgagcgg
     6841 ttcggctatg ccgtgacgga gatggagcgc tacgacgtcg cgccggcgcc tcggaccggc
     6901 gcctacgtgc tcagcagcgc tcgcagccgc ctcacgcttc ggcgggtcca cgtgcgcacg
     6961 cgggcgacgg cggccgcggt cagccgtctc ctcacaaagc gggcgcgcga cggcagctcg
     7021 cgacgcgtcc tggcggcgcg tagggccgga cgcgacgccg taaccgccgt cgtcctgacc
     7081 gtcccgcgct ccgctccgcg cccgacgaag cgccagctcg cccgcctgcg ccggctggcc
     7141 cgctcgctgc gcggcggctc ggcccggcgg ctgtcaaccc gcgaccgcga ctcccgcgcg
     7201 ccgaagcccg gtcgcggcaa gccaccgagg accgagccgg tcgagcctcc gacgaccgag
     7261 cccctcgagc ctccgaccac cgagcccgcc gagcccccgg cgagcgagcc ggtcgagccg
     7321 cccaccccgt cagagccagt cgagccgccc accacgcctt ccgagccggc ggggccgccg
     7381 acgacgccgc ctgacagcct cgcctccgga cagctgctct tcgaggacag gttcgacagc
     7441 ggatccgctg gcgaggggat ctggggcccc aagcaggcgg cgcccggatc gctcgactac
     7501 gtcgagagtc cggtgttccc cgggcgctct aaggcgatgc gcaaccgcct cgagccgggc
     7561 gacctccacg ccggcaccaa ctcctcgcgc agcgagctga ccggctcgct gaagttccgc
     7621 gatggcgacg tccgctggtt ccggatgccg gtccgtttca acgagtggtc gctgcagtcg
     7681 ggcgcctggg ctctgatctg gcaggcccac tccggctcgg gctcgccgcc gctagccgcc
     7741 tacgtccaaa gcgacgagct gctgttccga gtcaagaacg gctacaccgg acagatcttc
     7801 tgggagcaga agttcgagct tcaccgctgg tacgacctgg tgatcggaac gcagttctcc
     7861 cacggcggcg acggctgggt cgaggtctgg gtcgacggcg tccggcagaa gctgcgcaac
     7921 ggacagcagc ggatgaccgg ggcgaccctc tcccctacca gttcctacag ctacgacaag
     7981 ctcggcctct accaggcgtc gtcgttctca tccggcgcac gcgagctgct gcacgggatg
     8041 tacaaggtcg gcactacccg cgagtccgtc gtccgctagc gcggtcagcc gtcgcgcgcg
     8101 aggcggggcg tcctgtccgc gtcgcgccgg caggcggccg cgagcaccag cgccagcgtc
     8161 agcggcgcca cgtagccgat cgcgccgttg acgacggcgt agacgacgac cggtatgagc
     8221 agcatcgtcc gcagcccggc tcggaacagg gcggcccaga cgagcagcca agcgagcagg
     8281 ccgagcccgc cgatctgcac gccgacctcg atgatgtcgc tgtgcccgat atagggcacc
     8341 ccgagctcga agagctcggc ctctgggacc gagcgcaggc ctgccccgag cacccagccc
     8401 ggcgggcccg agtcggccca gaggtcgagc gcgacccccc agatcgccgt ccgcgaggag
     8461 ctcgccgtct cggccgtcgc gccagacctg aaggtctcgg acagccgctc ggtcaagacg
     8521 gcccccgccc caccggcgat cgcaagggcg agcgcgattc cgacaacggc caccgaccgg
     8581 cgatcggcgc gcgactcgat cagcatcacg agcaggatca gcgcgaccga cagcatcgcc
     8641 gacctcacgc cggtgagcag tgccggcagc gcggcgaccg tgaacagccc ggcgcggaca
     8701 ctgacccgct cgctcatcgt caacgccgcg gcggcaatga cgaccgacag cagcgccatc
     8761 tcgtgcgggg cctcggcggc gaagccgagc ttctcaccga cgccgtactt ggtgtcgccg
     8821 gcgccgacgc ccgaggccgc gaccaggagg tggacgacca tcgcggcaaa gccgctcgcg
     8881 aagacggcgt tgcggacacg aggaagctcg ttgcgtccgc gctcgctcaa cacggcgagc
     8941 gcaacgacgg ggaagatcat gtagctggcc gcctgcttga gctgctcgac gtcggtggcg
     9001 accaccgcgt gggcgagcac aacgacggcg aagacccctg cgccgatcgg gcctagggca
     9061 cgctggtagc gcaatggcag cgcgaaggcg acgagcgcca ccgcggcgat cccagtcgcg
     9121 gcggtgcgga ccagcggggg cacgaaggcg tccaggacga gcagccacgg caaggcgccc
     9181 agcagcagct gaaccagcac cagacgggag gcggtcacga cgacgatcgc agctccgagc
     9241 agcgccagca tgccgaccgc gagcgccggc gcgcgtgccg caagcgcgcc agtgccagcc
     9301 gccgcgagcg cggctagcgc ccacccgagc ccggcggcgc cgcgcgggcc ggggaccgtg
     9361 ccgtcgccgg gcgtgaagat cgggcggagg gtgacggggc ggcgcatggc ggtcgcgcgc
     9421 gcgcggcgcg cccgagccag tcaagcagac cgtcgcgacg caccggtcat cggtcgcccg
     9481 ctgcgagtag cgttccgagg ccgatgggcg accagcgaac gacgactccg cgccgagatc
     9541 ggcgcagggg ccgtgtgctg cacgtcacgc cggatcccca gcgcccaggc ggcatggcgg
     9601 ccgtcgtgcg cgacctgctc gcctcgccgc tgggagatcg ctacgcgctc gacgtgctgg
     9661 tgtcgcaccg gacgcgggac cggctcgggc ggatcgcggt cttcgtacgg gcgctctggc
     9721 gactgagccg ctggtgccgc ggcgccggga gccggatcgt gcacgtgcac agcgcggtgc
     9781 gcggcagcat gtaccgcaag gcgctctgcg tcgcggtcgc caagtcggcc gggcggcccg
     9841 tcgtcctgca gctgcacgcc ggtgcaggcg acatagaggc gttcgacgcg agcctcgggc
     9901 gcctgcggcg ggcattggtc ggccatgcgc tgcgccgcgc cgaccgggtg gtgagcgtgt
     9961 ccgcgccggg cgctgagcgg atcgagcgct gcttcggggt ccacggcgtg gtcgtgatcc
    10021 ccaacgccgc ccccgcgata cgcgcggcgc gcggcgcgcg cgaccccgat cggcccgtca
    10081 gggccctgta catgggcggc ttcgtcaatc ccgtgaaggg aggggcggtg ctgatcgagg
    10141 cgctgccgcg gctgctcgcc ggcgatccca acctcgaggt cgccttcgcg ggcacgggga
    10201 ccccaccgga cgagctgctg gaggcggctc gccgccacga gggggtcagc tggctgggct
    10261 ggctcgacgc cgaggctaag gccgagcagc tcgagcgctg cgacgtgttc gtcatgccgt
    10321 cgctgagcga gggaatgccg atcgccctgc tggaggcgat ggcctacggc tgcgcgatcg
    10381 tggcgagcgc cgtcggaggc gttcccgagg tcgtcgccga cggctcggag gcggtgctgg
    10441 tgccgccgtc ggatcctgcc gcgctcgcgg acgcggtcgc gcgcaccgcc gccgacccgg
    10501 cgctgcgcgc gcgcctgggc gcggcggcgc acgagcgcat cggcgacttc gacgccggca
    10561 acgtctacga ccgcttcgcc gctctctacg atgagctgtt ggcgcttccc ggagcgacgc
    10621 ggtgagcgcg ggccgactgc gcaaccgcag ccgtgaggcg gtctttctcg gctaccactc
    10681 ggtcgcggag gagggcccgc cgtttctgtc cgtcaccccc gaggcgttcg agcgccactt
    10741 ggcgctgctg aagcggcgcg gctttcgctc ggggacgctc gccgacctcg ctcacgtcgc
    10801 cggcggcggc cggccggacg gcaagctcgc gttcctgacc ttcgacgatg gctacgtcga
    10861 caactacgcg accgcgcgcc cgctgctgga ggcgtacggc ttccgggcga tcgtcttcct
    10921 cgtccccccg ctgatcgacg gcggcggacc gctcgtctgg gagcgcgtcg agcgccacgt
    10981 gcgcgactac cccgcggtga tgcgggcgat gacatggacg atggtcgagt cgatggccgc
    11041 cgagggacac gagttcggca gccacacgct cacccatccg cggctgccgg acctcgacga
    11101 cgagcgcctg cgtcgcgagc tgtcggagtc gcgcgagcag gtcgagcggc ggctcggaag
    11161 ctgcgaggcg cttgcgtatc ccttcggcct ttggagcccc cgcgtcgccg cagcgaccgg
    11221 ccgggcgggg tactcctacg ccttcacgct cccctacggc gcgcagacgc acgctgacag
    11281 gctcaccatc ccgcggatcg ccatcgacca tcgcgacgac gagcgccgct tcgggctcaa
    11341 gctgacgcgg gcataccggg ccttcgcgct ctcacgcggc aagccggccc tgcgacgact
    11401 gctgcggcgc gcaccggcgc accagtctcc tgcctgagcg gctccggcgt cgcctcgacc
    11461 ggctagcggc cgcgcaaccg ctcgcgcgcg accttggcga ggaagcgcga gttcgtcgtc
    11521 aggtagcgct tccagagacg tctcggctcc tggacgacgc gccagaacca ctcgaggccg
    11581 aggcgctgcc atagcgccgg agcgcggcgc acgacgccgg cgatcacgtc gaagctcccg
    11641 cccgacgcca cagccaaaga gacaccggtc gccgggccct gccggtcgat ccacaactcc
    11701 ttgcgcggcg acggcatgcc gacgaacatc acgtcggcgt ccgacttccg cacgatcgcc
    11761 gcaacctccg cgtcgcgctc gggtccgaag tagccgttgt ggcgccccgc gacgatcagc
    11821 cccggatggc ggcgccggca gtagtcctcg accgcttcga gcacctcgtc cctgcctccg
    11881 aggaagaaca cgcgccagcc cctgctgtcg gcgacctgaa gcaggtcgag catgtagtcg
    11941 atgccggcca cacgctcggg gagcggctgc ccgaggatgc gcgaggccca cacgagcgac
    12001 tgcccgtcgg cggcgagcag ggtggcgtcg aagagcgccg cgcgcagttc ggcgtcgctc
    12061 tccgccatca ccaccttggc cgcgttagcg aacaggacgg agttgaactc gccgccctcg
    12121 accgcgcgga cagcgaactc gcgggcgccc tccacgtcga tcaccgcgca agggagcccg
    12181 agcagcggcg gggcgtcgat gtcggaggtc gtaccgggca tcgccgtccg catcggcccg
    12241 gaggcgcgag cgatttagcg ctgggccgcg ccgttcatcg cggcccacgc ctcgaccagc
    12301 cagccgagct cgtacgggcg gcaggttcgg ttcagctcca gcctgcgccc ccggccggcg
    12361 cgcgagcgtc cgatcgccgc tgcggtcgcg gtgttggcgt ataggcagag gcggtcgagc
    12421 ggtcgcacgc gcctgatcga gcggtaggtc atcccgcgct ccaacggcgc catctgagct
    12481 ccgagctcgt tcgctccgaa gagccacggg atcccgcgat cgcccgccgc ggcgtagcgc
    12541 tcctcgccgc tgaccccgct cagctcgtag agcgtcatcg gcgccatccc gtgctggtgc
    12601 acggagtaga cctcgtagcg ctcgacgacc gatccacgct cggcgtcgta catccagggc
    12661 cagccgccgt ccggcagctg cagcgccaac aggcgatcgg cggcgcgacg ggccgctggc
    12721 agcgcccgcg cgtcgaggcc ccgtcccgcc gtgaccgcca gcgccaggac cgagtagatc
    12781 tgggtggcga agttggggaa gcgccgccgc gcgccgccct ctccaaagtg ccggaacagc
    12841 ccgctggggc cctggttgga ggcgatcagc tggtcgagcg ccgccttgcg cagctcgcgc
    12901 ccgcgcgccg tcaggtcctc ggtgactgcg agcgcggtga cgatccagcc gagctcgagc
    12961 ccctcgcgcg ccgccaggcc gccgttgaca tcgagcgagc ggtcgagccg ctcgaccagc
    13021 gtgttcgccg agcccgtctc cgcgcgcgac tcgacccaga gccgcagcgc gacatctccg
    13081 ggcgtgacct ccggccgctc gagcgccgcg tcgatcgcgt cacgcagctt gtcgggatcg
    13141 atcgagggcg cggcgcccgc gtgccgcgcg cgcagcagac cgagcaggac gatcagcgcg
    13201 tagcggaccg actcgccacg cggcgcggag tccccctccg cgacctcgta gcagacgagt
    13261 cccgacggga cgaccatcgc cggcagcgcc gccatggccg gctcgagcag ctcggcggca
    13321 cggctgtcga aggcgagccc ctcagcggcc atcgcggccc tcgaggtcga tgtcgtagat
    13381 ggtgaggcct tgggcgagga ggcggtcaag cacggtgaac atcgcgggta ttctgcacac
    13441 cagcagctct gatgctccaa gcggcaccgc ccgcatgaca ccctcggcga gcagctccgc
    13501 ccgcacctgc tgccgtgcct gcgagcggat ccggtgcgcc gcaaccgccg gatcgtgcac
    13561 cagcgtcgcc acccggagtg gctcgccggc ggcgtcctcg cagcgcacgg aaaccggctt
    13621 ctcgaggaag acgtcgatcg gaaaccggtc gccaagcagg cactcgaacg tgtggtagag
    13681 cgtgaacgcg tggacgtcgg tcccgaacca cacctggtac gcgtcccgct cgagcagccg
    13741 cgcaaacggc gtcccctcgc cgaacggcgt cagcgccagc tcgtgacccg cgaccagcga
    13801 ttccgcgcct ggaccgcgcg cggacaccga gtgggtcggg tgcaggctgc ggacggtgcc
    13861 gggcagctgc ctgaagcgct ccgagaccga gcccatcgac gacggcgtcg agcgcacgct
    13921 gaagcacggg tcgtcgcgca ggtactcgag cacgctgccg cggaacggga acgccggcat
    13981 ggcgaccagc gcctcggccc ccaacacctc gtcgagcgca cgcaagaccg tctcggggcc
    14041 gccctcgatc tcaccgaacg ggctcatcgc cgactggaag aagacgttgt cgcctcgtga
    14101 cagtccggcg gcggccaatg ccgcgccgat ctccgcgccg gtcacccgca ccgggtccaa
    14161 ccgtcgcctg acgcggtggc gcatgcgggc gcgggagcgc tgggcgcgac gcagcgctcg
    14221 caacgccggc gcgggcgccc ggcgcagcac gagcgccctc agggctccac gtgacatcga
    14281 ggcgtcagca tagacacgcc ctacagtcgt gtgaggcttg tgtcgatacg gccgcatgac
    14341 gcttttcagt ggagccgtgg cggtggcggt cgtcgcgctg gtactcgcgg tcggcgcgag
    14401 cgagcccgcc ggcgcgctcg tcctgaagca tcgcccgacc ggcttcacgg tgcacgcgcc
    14461 gccggacttc cgcgtccagt tcgacaagcg ctcgacccgt tacacggtca ggagccgcaa
    14521 ccgccgtgcc cggctcacat acacgcgcct tcgcaccaag cggtcgccgg cggcgatcgc
    14581 ccgctcgcgc gcggcccggg tcgcgcgatc tcagctgcgg gtggtgcgcg ccgggcgccg
    14641 ctacagactg cgcgtctcgc gccgccgcgt cgtggaggtc ggacgggccg gtcgcgggac
    14701 ggtcgcggtg tcgacgctct ccacgcgcgg acggcgtacg gcggctcggc tggcgctgct
    14761 gcggcggatc ggccgctcgg cacgcggagg cctcgcacgc aacaccggca cgacccacgg
    14821 gctgtcggtc gcctggcgca ccccgaccgc cggccagatt ctcgcaccgg gctcggccga
    14881 gcttcgcgtc gacgcgtcca cgccgtcgcg ggtcgcggag gtgcgcttcc tggtcgacgg
    14941 cgagcacctg cacaccgagt acgagacccc ctacacgggc gtcctcgaca cgtccggact
    15001 gtcggcggga ctgcacaagc tgcgcgccgt ggtcgtcctg cgcgacggca gagcgatcgt
    15061 gcgcaaccag accgtcgcgg tgcgcggcgc cggctccgca ggcgagacct cgcccgccgc
    15121 cggcggcgga tgcgcgtcca acgcactgtt cgagggcggc ttcgagggcg gcctcgacga
    15181 gtggggaaac gtccaggcgc tcggcccacg ggcgacgatc gtccagggtg gctcgttctg
    15241 cggagcgagg cacggtcgct tcgaggttcg cgccggcgac gtggagcccg acaccgacag
    15301 caaccgcgcg gaggtcgccg gaccgtcctt ctccgacggc gacgacctct ggttccgcca
    15361 cgcgaccaag ctcgccgcgg gattcccgcg cgaggacaag tggcagatcg tcagccagtg
    15421 gaggtctggc gacggctctc ccccattggc gctcttcgtc agcgacgacc tggcgctgtc
    15481 gctgaggcgc ggcgacgcgc cctacaccga gttctggcgc ggcccggtcc agacggaggt
    15541 ctggaccgac ctcgtcttcc acatccgctt ctcgcgcaac cccgcgctgg gcttcgtcga
    15601 gctttggcgc gatggacggc gtcagacgat gcgcaacggc gccgagcgga tgagcggtca
    15661 gacgatcaag ggctcgagcg ggtacgtcaa gctcggctac taccgcgatc ccgatcacgt
    15721 cgggaccggc gtcgtctatc acgacaacta cctcgtcggt cgctcgctgg ccgacgtcgc
    15781 gccagccccc tgaccgccgg gcgcgggcgg catcccgtcg cgccacgata ctgcgccgat
    15841 gccgctgcag cgccttagag gactctccac cttcaagcgg ggcttcgcct cgaccttcac
    15901 tctcgacgtg ctggcgcggg cgatgagcgc aatgacgctc gtgctgctgc tgcgcgcgct
    15961 gccggtcgat ggcttcgcgt tcgtgatcct gctgctcagc atcgggcagt tcatgggcag
    16021 cgccgccacc ggcggcctgc gcatgcgcta cgtgcgcatc caggcggagc gggtctcgcg
    16081 cgagtcggat gagccgtcgt ccttctacct gacgctgcgt ggcggaatcg tgctgatcgt
    16141 cgccgccggg gcactcggat tcctgggcgc caccgcgctc gacgtgggaa cgcccgagga
    16201 gcgagccgtg atggcgttga tcgccaccgc ctacacgctt ggccatgcga gcattgagct
    16261 gagcgcggtc cactaccagg cacatgtcgc cttcgtgaag gccggcgtcc tgatctggct
    16321 gcggagcttc gtccagttcc tgttcgcggt cggcgcgacg gccgggctgc taaagaccgg
    16381 gaccgccgtc ggcgcggcct ttgcgatcgg cgtgatgtgc gtggcggtcg cgacggccgg
    16441 cccgccggcg tgggcgacgc gcaagtcgac cgtcgaccgg gagggccggt tcggcttcgg
    16501 gcgcgagtcg gccgcgctga cgctgttctc gattgcgacg gccggtcggg cctacatcaa
    16561 catcttcctc gtcgcagcgc tgctcgacga cacggccgtc acggtctacg gaaccgcaac
    16621 gcgctacatc tcagtcgtca tggggcccgt gcccgcgctc atgaccgtga tgcgcgtgcg
    16681 aacggcccag agcgacatgg tcgactcgca cgagacgcag ctgcgcctga tggtcgactg
    16741 ggccaggcgc agcctggtcc cgctgatcgt cgcgctcgga gcggccgcca tcgcggcgcc
    16801 gttcgtgatc ccgctgatag acgagggcaa gtaccccgac accgttcccg tcttcgagat
    16861 catgctcgtg atggcgttcg cgcagctgat gacgctgccg aacccgagcc tgttgctgac
    16921 acagaaccgc tactcgacgc tcgcctgggt cgatgcgacg gtagtcgtcg tcaacgttct
    16981 gctggcgctc gcggtggcgt cccatttcgg cgtgatcggc atcgtcgtcg tcgcggcgct
    17041 ggtcacggtc ggccaggtct gcgcagtgac ctacctcgcc gcccatccgc ccaagggcac
    17101 cgctggcgcc ccggccgccc cgctctcgga gggactcgag gagcaccccg agtcggcggt
    17161 gcgcgatccc tagccgcgag cgcgcacgcg gcggtaggcc cgtcgcagcg gcggccggat
    17221 cgaggtccgg tacgcgtagc gcgcgacgcc gagcggccgg cgcgggtatg cgcgcacgcc
    17281 gacgtggggc cgctcgcgcg tggcgaactt ggccttccac tcgctcctgt gaccgagcag
    17341 ctcgtgcgcc tcgaggccca gctcgaagca gcgctcgacg gtcgacaggc gcagaaccag
    17401 gcccggcgcc agcttgcgga agtcctcgtc gaaggcggtc ttgaccagat agagacggcc
    17461 gcggtcgagc aggcagaggt cgaacgccgc cgtcctgccg tcgagcacga tccggctgag
    17521 ccggagcgct ccgcgctcgt ggaaggcgcg gctcatctcg acgtagaagg catgggtcgc
    17581 gggatcgcag aggatcgccg tcccggcctg gcccttccag ccgctggcct caacgcgcag
    17641 gccggccgcg agctcgccat cgaggtcgac cggcggctcg acgatcgtca tctccgcgtc
    17701 gtagtcgcgc tgcatcttgc gccggaagcg ttccagcgga gcaccccagc ggcgcttgct
    17761 atccgcccgc caggcgtcga agtcgccaga cgtgtcgacg atcggcgaga catgctcggg
    17821 ctcgctggca accagcctcc cgctcgagcg tccgagctcg cacaggtccc cgatgatcgg
    17881 ctcgtccgcg gggatccgcg acaggaacag ctccggggcg cgccgctcga gcagcgcgcg
    17941 agccagcgcc ctgcgtgcgt cggcgtcgac cgcaagcagg cggaacatcg acgactgctc
    18001 gttcgccatc gccaggatgc ggccgctgga gtcgcgcatc agcggcgcga cggcgacgat
    18061 ttggtggccg cgccggacga cgcagacagc gagctgccga tcgcccccga acgccttcca
    18121 ccacgccgcg taccagcagt gcaggtcgaa ggggtgcgag tcgggcgtca gcagcgcgtc
    18181 ccagggctcg gcgagcgctg caaagccggc ctcgtcggcc acccagtcga ccgtcgtggc
    18241 ggcgggcgac ccggccatca acgtcgaacc aaccctcgca cgcgccgctt ggcctcgggc
    18301 gagagccgct tggcgagctc gccgcgcgcc tggtacgggg cgagccgcgc ccgcgcgagc
    18361 gggtatgccg ctccgcgcgg gacgagcgtc gtgaggctga tctccgaacg ttcgtcggcg
    18421 agcgtgccct tgtatggctg atctccgggc cccaggctaa tccgccgctc cccgcgctgc
    18481 gcggcgtcgg cgacggcctc gagcatcgtg aggatcgacg ggccgtatcg gctccagctc
    18541 gcatcgaagc cgccgttcca gccgctcacc tccgagccgg cggcgatgaa cacgtgcgcg
    18601 cagatcacct cgccgtcgac gtcgatcgtg aagagtcgga agcgctcgct gggaagcagc
    18661 tcgcggccag cctcggcgag catcggctcg atcccgtcga ctagtgcgtt cgatccgccc
    18721 cggtagtccc agcggtcgcg atgcaggcgc aggaacgccg cgagcgcgct gtcgaagtcg
    18781 ttctcgccgg ccagcgagaa gcggccgccg gccttgtcga gctggcgtcg gcggcgcctc
    18841 gtctcctggc ggaacttcgc gttgcggccc tgtagccagg cgtcgtagtc gaggccgccg
    18901 aggacgatcg ccggcgcggc catcgccgcc ggggagtagc gccacggccg tcggcccggc
    18961 caggcgtcgg ccaggctcat ggcccactgc gtggagtcgc tctcgtcctc gagctgcagc
    19021 gcgtgcgggc gtggtgtcga gcgcgcgagc gccgacgcga tcgcagcggc gacctcgttc
    19081 tcccgaccgg tcgcggcgag cgggcggacc ggcggcgaaa gccgactggc cagcatcgcg
    19141 tagcgcgtgc cgccggcgcg gtcgccggcg acccagagcg gtgcgaggcc gatcagccgc
    19201 tcgccctcga cgacgaccac ggcgcgcagg agcgcgtcgg cgggacgcgc atgccgccac
    19261 caagccagca tccacgccgg gctcgaatac ggctgcccgg tctcgacagc cagtgcgtcc
    19321 cactgagagc ggaaggactc caggtcggac tccaggtcga gaatctccac cgccaccggc
    19381 acgcgcgcag actacacgtg gccaccgagc ccgcagtttg cgggacttca ggggtcgtga
    19441 tcgacggatg tttgaaatcc gtcggcgcca gtcactatgc tcgcgtcgcg atcaaatgcc
    19501 gagtcgctcc cgatccgacg ggagcgagcg ggggaaccaa ttcctttggg gctaatcgcg
    19561 gtgctggccc atatgcgccg tgacgcgctg ctctttctgc gcgagcccga cagctaaccc
    19621 cgtaggccta ggaaagggga ggctcgcgat caccgcgctg cgcacattca tccttgccgc
    19681 cttgctggcg gctctgcttt tgccatccgc tgcagccgcc aagcaatccg accgcgagtc
    19741 ggcggcggtg atggtcgacc ggatcaactc gacacgggcg aagcacgacc tgccctccct
    19801 gcgcgcggcg ccccgcctga tgcgctccgc gcgccgctat gcggcccacc tgatgcgcgc
    19861 cgacgccttc gagcacgggc cggcgcgcat cacgggattc cgcgcatccg gcgagatgct
    19921 ggctttcaac ccgggctggc ggctaaggac ctcgccggtc gtccgaatgt ggctcggctc
    19981 gccgggacat cgcgcgctga tgctcagccg atcgttcggc cacgtcggcg tcagccccgc
    20041 ccgcggccgc ttcggagggg cgctgacgac gatctgggtg gttcagctcg gccgccgcta
    20101 ggcgccgggc tcccgccgcg gcaccagcac cgagcgcttg aactcggcga cgagcacgcc
    20161 gtcctggttc aggacgcgcg tgtgcacacg cacgacgccg cggtcgggct tcgagctcga
    20221 cgggcgcacc tcgaggacct cggactcgca gaacagcgtg tcgccgtgga agaccggcgc
    20281 cggatgcgat agctcgtcgg tggcgaggtt ggcgatcgcc ttgcctgaga cgtctccgac
    20341 cgacatgccg agcgcgagcg agtagacgag cgggccgacg acgacgttgc ggccctgctg
    20401 cgactgcttg gcgtagacgt cgttgatgtg cagcgggtgg tggttcatcg tgatcaggca
    20461 gaacatgtgg tcgtcggcct cggtcacggt cttcgccggc cagtgcttgt agacggcgcc
    20521 gacctcgaag tcctccaggt agcgcccgta ggggcgggcg ccggaggcct cgcgtgtcgc
    20581 ctggtctgtg tcgacggtca tgtgctcctc gagtcggttg attcgtcagt caatcggaag
    20641 gcgcggaatc gtaaccggcg gtaggttttg atgcccatgc gcaatccacg agacttcttc
    20701 aagccgctcg ccgtcggcgc gccggacccg gtagccgagg tccccttcaa gccgtcgcgg
    20761 atgatccact tcctcgactt ctcgaacgag aagatggtcg ccaaggcgcc cgcgatcgcc
    20821 ggccaggtcg acatcctgct cggcaacctc gaggacgcga tcccgatcga ccgcaaggag
    20881 gcggcgcgcg aggggctgat caccgtcggg cgcgacatgg agctcggcga cacggccctc
    20941 tgggtgcgcg tgaactcgct cgagagccca tgggtgctcg acgacctgac cgagctggtc
    21001 gcgacgatcg gcgacaagct cgagacgatc atggtgccga aggtcgaggg cgcctgggac
    21061 atccactacg tcgaccgcct gctcgcccag ctcgaggcga aggccggcgt cgagaagccg
    21121 atcatggtcc acgcgatcct cgagaccggc atgggcgtgg tcaacctcga ggagatcgcg
    21181 accgcgagcc cgcgcatgca ggggatgagc ttcgggcccg ccgacctcgc cgcctcgcgc
    21241 cgcatgaaga cgacgcgcgt cggcggcggg caccccggct accgggtgat cgaggacccc
    21301 gaccccgacc agcccgacgc gccgcggatc accgcccagc aagatccctg gcactactcg
    21361 atcgcgcgca tggtcgacgc ctgcacctcc gcggggatcc ttccgttcta cggcccctat
    21421 ggcgacatca aggacgtcga cggctgcgag gcgcagttcc gcgccgcgtt cctgctcggc
    21481 tgcgtcgggg cctggtcgct gcacccggtc cagatcgaca tcgccaagaa ggtcttctcg
    21541 cccgatccca acgaggtcgc cttcgcgaag aaggtgatcg aggcgatccc cgacggcgcc
    21601 ggcgtccaca tgatcgacgg caagatgcag gacgacgcca cctggaagca gtgcaaggtg
    21661 atggtcaacc tcgccgagat gctcgccaag aaggaccccg agctggccga ggcctacggc
    21721 ttcgagcgcg cgacggcggg tgccgcgtca tgaagttcta cgagtacgag gcgcgccaga
    21781 tcgtcgagca ggccggcatc ccggtcacga agtacggctt cgccaagacc gccgaggagg
    21841 cgcgcgcgat cgcggccgac atcgacggcc cgacggtgat caagtcgcag gtgctgaccg
    21901 ggggccggat gaaggccggc ggcgtcaagt tcgccgacac gcccgacgag gcggccgccc
    21961 acgccgagga catcctgaag ctggagatca acggccacat gccgcgcggc gtgctcgtcg
    22021 acccgaaggc gcaggtcaag caggagtact acgccggcgt cgtctgggac ggcacgcgca
    22081 agcagccgct gctgctgttc agcgacatgg gcgggatcga catcgagcag gtcgccgagg
    22141 agcaccccga ccacgtcggc cgcggccact tctcgaacct cctcccctac tccgacttcc
    22201 aggccaagca ggtgatcgcc gccgccggcg tcagcggcaa ggagctgacg cggctgacgc
    22261 cgatcctgac gcggctcgcg cggctgttcc agacctgcga catgacgctc gccgagatca
    22321 acccgctcgc ccagctcgag gacggcagct tcgtcgcgct cgacgcccac atggagatgg
    22381 agaacgaggc gatgggccgg cagaagaagc tgctcgagag catcggcatc ggcgccgagg
    22441 agggcacccg cgaggggcac gagccgagcc cgttcgaggc gatcgtcaag cagatcgact
    22501 ccgccgacca ccgcggcgtg atccagggca aggacaccgg cttccacggc aacgtcgggc
    22561 tcgtgatcgg cgcgggcggc ggctcgctga cgctgaccga cgccgtccgc tcgcagggcg
    22621 gcaagccggc gaactactcc gagatcggcg gcaacccctc ggtcgccaaa gcctgcggcc
    22681 tcgccaaggc ggtgctgcag aaggagggcg tcgagaagat cgcggtgatg atgtcgatcg
    22741 tctccaacac gcgcgtcgac atcgtcgccc gcggggttat caaggcctgc ctcgagctcg
    22801 gaaaggaccc cggcgagacc gtcgcgatct tccgcatccc cggtgcctgg gaggacgagg
    22861 gcttcaagat cctcgacagg tacgggatcg agtactgcga ccggtcggtc tcgatgcacg
    22921 aggcggccag gcgggccgtc gagaagatcc agggagaggg cggcccgaag cagccggctc
    22981 tgccggatgc gcagggaaag gtgtcggcat gagcatcctg atcgacgaga acacgacctt
    23041 catcgttcag ggcatcaccg gccgcgaggc cgtcaacctg acgcgcgagt gcctcgacta
    23101 cggctcgaag atcgtcggcg gcgtgacacc cggccgcaaa ggccgcgacg tccacggcgt
    23161 gccggtcttc gacaccgtcg agcaggtgat cgcccacaac gacggccgcg tgcccgacgg
    23221 ctcggtcgtc accgtccccc ccgcgttcac caaggacgcc gtcttcgagg cgatcgagaa
    23281 cgggatcaag ctgatcgtga tcgtcaccga gcgcatcccg cgccgcgacg tcgcgcagat
    23341 ggtcgagctc gccaacctgc gcggcgcgcg gatcatcggg ccgaactgcc tcgggctgat
    23401 cgtcccgggg gtcgccaaga tgggcggcat cggcggcccg gccaagaacg ccgcgcaggc
    23461 ctaccagccc ggctcaatcg gtgtgatgtc gcgctccggc ggcatgacta ccgagatctc
    23521 ctcgacgctg acggctgccg gcctcggcgt gtcgacagcg atctcgatcg gcggcgacgc
    23581 gatcatcggc tccgcctatg cggagctgat gccgctcttc gaggccgacg agcagaccga
    23641 ggcgatcgtg atctacaccg agccgggcgg ccggatggag gcccagctcg ccgagtgggt
    23701 cgccgagcac gactcgcgcc tgccgatcgt cgcattcatg gccgggcggt tcatggacga
    23761 cgaggagatg caggggatgt ccttcggcca cgccggcacg atcgtcgagg gcaaggagga
    23821 caccgcgacc gagaagatcg cccgcctcga gtcggccggc atcccggtcg tcgagcggat
    23881 cgacgagatt cccgacgcgg tcaagcgcaa gctcgcggat cgcaagtccg agaagggagc
    23941 cgttgcctga tgtcccgcat cttcatcgac atcgaggtcg ccgacgaggt ccgcgacgac
    24001 accgagctgg cgcgcaagct ggccgaggtc tgcccggtcg acatctacgc cgagcgcgac
    24061 ggcaaggtcg ccgtcgtaga cgagaacgtc gacgagtgcg tgctctgcgg cctctgcctc
    24121 gacgcctctc cgcccggcgc cgtcgccgtc aagaagctct acgacggcac gacgctgacg
    24181 cgctagccgg ctcgggcgcc tttgttgtga gcggcgaccc ctacactgcc cgggcatgac
    24241 cagcccgaac ggcaagccgt tctcgcgcgt cggcgtgatc ggcctcggct acgttgggct
    24301 gccgctcgcg gtcgccttcg cggaggaggg cgtcgacgtc gtcggcgtcg actacgcgcc
    24361 gcagctggta gcggcaatcg aggccggcga gagccacatc gaggacatcg cgtccgagcg
    24421 gctccaggcg gtcggcgacc ggatcgcggc gaccaccgac tacgggcgcc tggcggactg
    24481 cgacgcggtg ctgatcgcgg tgccgacccc gctgacgcgc aaccgcgagc cggacctcgg
    24541 accgctgctg tcctcggcgc aggcgctgtc aggcgtggtt cgcaccggcc agctcgtggt
    24601 gctcgagtca accacctacc ccggcacgac gcgcgagcag ctggtgccgc tgctcgagga
    24661 gtcgggactc gccgccggac gcgacttcaa cgtcgcgttc tcgcccgagc gcgtcgaccc
    24721 cgggcggacc gaccacacgc tgcgcacgac gccgaagatc gtcggcggcc tcacgccggc
    24781 ctgcctggcg cgagcggtcg cgctctacga gatcgtctgc gacaacctcg tcgaggtctc
    24841 ggccccggag ccggccgagc tcgcgaagct gctcgagaac gtcttccggt cggtcaacat
    24901 cgcgctcgtc aacgagctgg cgatgctctg cgaccggatg ggcatcgacg tctgggaggt
    24961 gatcgacgcc gccgccacca agccctacgg cttcatgcgc ttcgatcccg gccccgggat
    25021 gggcggccac tgcctgcccg tcgacccgtt ctacctctcg tggaaagcgc gcgagtacgg
    25081 ccagccgacc gagttcatcg agctcgccgg cgaggtcaac cagcggatgc ctgagttctg
    25141 cgtcgaccgg atcgcgcgcg cgctcaatga ccactccaag ccggtgcgcg gctcgaagat
    25201 cgcgatcgtc ggcgtgtcct acaagtccgg cgtcggcgac ctgcgcgagt cgcccgccct
    25261 gcggatcatg gggctgctcg cggagggcgg cgccgtgctc gcctaccacg acgacttcat
    25321 cgccgagatc cccgagctcg gctacagcgg cgagacgctc gatgcggcgc tcgacggcgc
    25381 cgacgccgcc gtgatcgtga ccgcccaccc cggcctcgac ctcgagcgga tcgtcgcggc
    25441 ggcgccgctg gtggtcgact tccgcggggt cacgcgcggc atcgaggcgc cgaacctcgt
    25501 ccgcctctag cggtcggcta gccgaccgcc ggcgccacgt cggccgccca ggcgagcagc
    25561 tcggcgacgt gctcgtcagt gatgccgacg gcgggctggg tctcgatcag cagcgtcggc
    25621 tccgaccgcc cgcgcgccca ggcgaggcac tcgtcgtcga cgttgtcgtc gatccacgcg
    25681 gccggccgcc cgcgggcgta ctcgtcgatc gcctcgacct tccagtgcga gctgccgaag
    25741 cgggcgcgtc cgtcgaaggt caggcagggc agctcgtgga acggcaggcc gaggatccgc
    25801 ggcaggtact cgttcgcctt ctcctcccag ccggtcgccc acacgagctc gaagcggtcg
    25861 gcgaggcgtg cgagccgcgg gcccgagtcc ggcgggatgc agtgggcgat accgtcgatc
    25921 cagtggaacg ggccgggcag cccggagccc ggatcgaagc cgaagaggct gaggacgccg
    25981 tcgacgtcga cgaagaggac cggcttcacc gctacttggc gcgcaccagc tgtcgctcca
    26041 tcacgttgcc gagcagcttc accaccgcgc tgtcgccggt caccttgacg tggccctgct
    26101 cgtaggcctt ggcccactgg cgcagcgtgc cctccgtcgc cagctcgttg aactgctggc
    26161 gctgcacgac cacggacagc cgcgcgtcgc ccgcgggatc gcgctcgacc ttcggccccg
    26221 gcacctcgac gcgccagatc ggggcgtcgg agccacgacc cggcagctcg agccggacga
    26281 ccagcttgag ctggcgcagc gacggcacct ccgtcgtcag ctgctcgatg gcgtcggcta
    26341 tcagggcttt ggtatcggtg gcggacacgc caccgagtct agtcagcagg cgtcttcgcc
    26401 agcccccgct tcacttcttg aatgcgcgca gcgaccggcg tgccgcctcg cgccctgcct
    26461 ttcgcagcca ggcggccagc cccctcgggg tgcgcctgaa cgcccccatc cagctccgct
    26521 cgaccgagcg atcggcccaa ctcgacttga acgcgtcctc gttgcccaac aggtcgagac
    26581 aggtcacccc gctcgccagg cagtggccga tctcctcgcg cagcagcagc gcgccggggc
    26641 cgaaggaacg gaactcctcg tcatagccgg ccttgagcga gtaccaggtc ccgccatgct
    26701 cgatcacgta gtcgcatgcg atcgcgcgac cctcgagacg caggcaggcc agacgcagcc
    26761 agccgccttc agccgcccag cgtgcgacag ccgtatagaa gcgcttcgtc tccggctgtg
    26821 actggatcgc cgttcctcga tcgcccttcc agcccgacgc ctccacgcgg tacaggtcgg
    26881 cgagcagctc gtcgagacgt tcgctgccgt catggacctc gaaggtcagc tcgccgagct
    26941 gctccagacg gcgacgctgg cgcctcagag cgcgccggcg attgcgtgac agcgtctgct
    27001 cgtattccgt cacacatcct gtggtgtcga ttatcggcga gcgcgtgacg gcccgactca
    27061 agacgactcg ccgttgccgc cgcgctgccg cgaccaacgt ctccagcggg gcctgagaat
    27121 cgcagagcag attcatctcg acgcgcgcgc tgggctcggc gaacacacgc tccatcagct
    27181 cctcctgtac ggatcggtcg acggcgagcg ggccgaacat cggagagtgc cagttcaccg
    27241 gggcgaggag cgcgccggcg cgaacctcca tcggcaggat tccagccagc tcaccccctc
    27301 gacgaacggt tatcggctcc ggcctgccgc aggcaaaggc ctccgaccac gcgctgaacc
    27361 accccggacg tagaaacggc ggaacgtcca gagaattggc gagatactcc cactccgcag
    27421 tgagcggagc ctctgagggc ggtagcaacg gacctccaaa aactggtcga caaaatgaac
    27481 tgccgaccag ccttcccggc gcaccacgtg cgaccctacc agtgggcgca gatgggggcc
    27541 agctctagcg gctcgcccac acctcggact gcgcgcatcg gcgcctcgcc cttcagcagc
    27601 gcctcgtgga actcgtcgtc ggcgttcgac tgggtgacga tcgccccgcc gacgccgatc
    27661 gtcgccttgc cgtcggcgac cacgatcgtc ctgatcgtga cgctgagatc gcagctgccg
    27721 ccggggccga ggtagccgat cgcgcccgag tagacgccgc gcggggcctc ctcgagctcg
    27781 tcgacgatct ccatcgtgcg cttcttcggc gcgccggtca tcgagcccgg cgggaagcag
    27841 gcacgaatgc agtcggcggt atcggtttcg ggctgcagca ggccgcgaac cgtcgagacg
    27901 agctggtgaa ccgtctcgta ggtctcgacc tgcatcaggt gcggcacgtg gaccgtgccg
    27961 acttcgcaga cgcgaccgag gtcgttgcgc aagaggtcgg tgatcatcag gttctcggcg
    28021 cgatccttct cgctcgtccg cagctcctcg gcgagccgca cgtcatccac cggcgtcagg
    28081 ccgcggcgtg cggtcccctt gatcggcttc gcctcggccc agcggtcgcg gccgacgcgc
    28141 aggaagcgct ccggggagga gctcagcacc gcgaccccgc cgaagcgcag gaaggcggcg
    28201 tacggggccg ggttcacgcg ccgcagctcg cggtagagca ccagcggatc gccgtcgaag
    28261 tcggtggcga tctggttggt caggcagacc tcgtaggtct cgccgtcgaa cagccgctcc
    28321 ttgcagaccg cgatgtcggc gagatagcgc tcgcgcgagc gggcgaggga aaactcgccc
    28381 ggcggggcgg cggcccgcgg ccgctcgtgg gccggtgcgg ggccctgcgg gccgctgcgg
    28441 gaggtgagat cgccaggcgg cgggagcgac tcgaggcgct gccgggtgtc agcgatccag
    28501 cgctcggcat cgtgcgtgtt gacgacgtcg gtcaggcaga gcaggtaggt gtggccctcc
    28561 tcgtggtcga acgcgagcat ccggtcggcg cggacgaagg cggcgtcggg gagcggcgag
    28621 tcgtagaccg catcgccgcc gcagtcggcc ttcagctcgt agccgaggta gccggcgaag
    28681 ccgcaggcga agtcgaacgg gaggtcgggc ggggccgcga cctcgcgaag ccttgcctgc
    28741 tcgcggcgta ggtagtcgaa gatcgtctct cggtgacgct cgacgccgcc cgcggcggtg
    28801 acggtcacct cgcccgcgtc gacgtcgtac gtcacgaccg ctccgaacgg accgctcgcg
    28861 tcgccggcga acgagaagcg cgcctcacct tcgacccggg cgctgtcgag ccagaacgcg
    28921 tcggcgctgt cggcgtacag agcggcgaac gccgcctccg ggtcggcgag catgtcgagc
    28981 cgctcgacga ccagcgacag cggatcgctc ggcgacgccc tttcccccga atcgttcgac
    29041 tccacggggg ccccggacac tgagccctgt tcggcgacat cgccgccgag atcgacccgc
    29101 cgtggcgatg gcgccgacgt tgggtgtgtt tcggcgacat tcccgccgga atcggcccgg
    29161 cgtgggcgcg cggcggcggc agtgaggtcg cggaagttgg ccagcagccg acggccgtgc
    29221 tgggtcgcga tcgactcggg gtggaactgg acgccccaga gcgggcgcgt gcggtgcgca
    29281 accgccatca gaacgccgtc gctcgcccag gcggtcggct cgagctcgtc gggcagcgcc
    29341 cagccggcgc agagcgagtg gtagcggacg gcctggaact cgcgtgggat gccgacgaag
    29401 agagcggagc cgtcgtggat caccgcgccg gcgcgcccgt ggacggcctc gggcgcatgc
    29461 tcgacgcgac cgccgagggc agagcagatc ccctgatggc ctagacagac tccgagcagc
    29521 ggtacctctg cgcgccggac cgcctcgccg cagacgccga agtcggccgg atcgtcggcg
    29581 cgacccggcc cgggcgagat cacgacgttg tcgaagtccc gccgcgccag ttcctcccag
    29641 tcggccgcgt cgttgcacac gacctcgggc agctccccgt tcacctcggc cagaagctgg
    29701 aagaggttgt aggtgaacga gtcgtagttg tcgatcagca gcgtcttcat cgtcgtgacc
    29761 cgcagcctgc gatcctcgca ggccgtgacg gccgacagtc cgctctcgac cactcacctc
    29821 ccccgccccg accctcggcg cggcatcttc gagacgctgc tcgtcgtcga cggccggccg
    29881 gtcgagctcg acgcccacct cgcccgcctc gccgccagcg cccgcgaggt cttcggcccg
    29941 ccggcccggt cacccgcgcc atcgccggcg ggccgaagac ctcgcgggcg ctggcggcga
    30001 ggcgggcgag gtgggcgtcg agctcgacgg cgcgaccgtc gacgacgagc agcgtctcga
    30061 agacgccgcg cgcggggtcg gggcggagga gatctgccgt cgagagcggg ctgccgaccg
    30121 tcacggcctg cgaggatcgc atgctgagcg gtgagacgat gaagacgctg ctgatcgaca
    30181 actacgactc gttcacccac aacctcttcc agctgctggg cgaggtcaac ggcgagcagc
    30241 ccgaggtcgt ccgcaacgac gaggcgagct gggacgagct ggcgcggctg cctttcgaca
    30301 acgtcgtcat ctcgcccggc ccgggacgac cggacgatcc gcaggacttc ggcgtctgcg
    30361 ccgacgcgat ccgccgcgcg gaggtgccgc tgctcggcgt ctgcctcggt caccagggga
    30421 tctgcgcggc ctacggggga cacgtcgagc gcgccgccga agcggtccac gggcgcacga
    30481 gcgcggtgct ccacgacggc tcgccgctgt tcgccggcat cccgcgcgag ttccaggcgg
    30541 tccgctacca ctcgctcgcc gccggctggg agctacccga agagctcgag ccgaccgcct
    30601 ggaccagtga cggcgtcctg atggccgtcg cccaccgctc acgcccgctc tggggcgtcc
    30661 agttccaccc cgagtcgatc gcgaccgagc acggccgtcg cctgctggca aacttccgcg
    30721 acctgacgcc tgcgcgctcg ctcgaggctc gcggcaagcc gtcgccgtcg ccgtcgcggc
    30781 gagtggcgag ttgcggacgc aatacgtccg c
//
LOCUS       DATABV010000075         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2032406, whole genome shotgun sequence.
ACCESSION   DATABV010000075 DATABV010000000
VERSION     DATABV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032406"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..>1565
                     /locus_tag="VHF88_03125"
     CDS             166..>1565
                     /locus_tag="VHF88_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2160791.1"
                     /translation="MTAVRRHPALAIGVVVVAVAIALYAFGAMPDFPDVKKLVGDIAQ
                     ALGKWTYLLVGLLAFLETGAFVGLVAPGETVVIVGGVIAGQGEISLIVLIGIVWSAAI
                     LGDTTSFFIGRRLGRGFILRHGPKVKIDESRLEQVEAYFERHGGKTIVIGRFIGLVRA
                     LAPFVVGSSGLAYRRFIPYSVIGCGLWATTFSVLGFVFWQSFDQVAGIAGKATFGFGL
                     TVAAIVGAVLAYRQLRRPEQRRRAVEWMEGKPPLRPLLAVGRPIHRRVIAPAWNVVGP
                     QVRFVFNRVTPGDLGLELLELADRLNVHDLLTDVAKVVTDFGAFPTAAALVLATSILL
                     AARRHWYEAAALTLGFALLYVSVHLAKNGIDRPRPSAPLTDADGAAFPSGHAAYSTAW
                     VAVAVVVARVIPGIASRAALIGGALALAAAIGLSRIYLRVHYWSDVAGGWGLGAGLFG
                     ACAVIGLVVAHVRHNG"
BASE COUNT          176 a          567 c          570 g          252 t
ORIGIN      
        1 gagcgacgcc ggggcgctcg cggcccgggt caccgggtcc ggtccgacga cgttcggcgt
       61 cttcgcggac cgcgccgcgg cggagcgagc cgcggcggcg atcgacacgc gtgcagcggc
      121 agacggcgat gcgtcgcccc ccgcgatcgt ggccggactg cgcccgtgac cgccgtccgc
      181 cgtcaccccg cattggccat cggcgtcgtg gtcgtcgcgg tcgcgatcgc gctctacgca
      241 ttcggcgcga tgccggactt ccccgacgtc aagaagctgg tcggtgacat cgcccaggca
      301 ctcgggaagt ggacctacct cctcgtcggg ctgctcgcgt tcctcgagac cggcgccttc
      361 gtcggacttg tcgcaccggg cgagacggtg gtgatcgtcg gcggcgtgat cgccggccag
      421 ggcgagatca gcctgatcgt gctgatcggg atcgtctggt cggcggcgat cctcggcgac
      481 acgaccagct tcttcatcgg ccgccggctc gggcgcggct tcatcctgcg ccacgggccg
      541 aaggtcaaga tcgacgagtc gcggctcgag caggtcgagg cctacttcga gcgccacggc
      601 ggcaagacga tcgtgatcgg ccgcttcatc ggcctggtgc gcgcgctggc gccgttcgtc
      661 gtcggctcgt ccggtctggc ataccgtcgc ttcatcccgt acagcgtcat cggctgcggg
      721 ctctgggcga cgacgttctc ggtgctcggg ttcgtcttct ggcagtcgtt cgaccaggtc
      781 gcgggcatcg ccggcaaggc gaccttcggc ttcggcctca cagtcgcggc gatcgtcggc
      841 gcggtgctcg cctaccgcca gctgcggcgc cccgagcagc gccgccgggc ggtggagtgg
      901 atggagggca agccgccgct gcggccgctg ctcgccgtcg ggcgaccgat ccaccgtcgc
      961 gtcatagcgc cggcatggaa cgtagtcggg ccgcaggtgc gcttcgtctt caaccgcgtg
     1021 acgcccggcg acctggggct cgagctgctc gagctcgcgg accggctcaa cgtccacgac
     1081 ctgctgaccg acgtcgccaa ggtcgtcacc gacttcggcg ccttcccgac ggctgcggcg
     1141 ctcgtgctcg cgacgtcgat cctgctcgcg gcgcggcggc actggtacga ggcggcggca
     1201 ctgacgctcg gcttcgcgct gctctacgtg tcggtccacc tggcgaagaa cgggatcgac
     1261 cggccgcgcc cgagcgcgcc gctgaccgac gccgacggcg cggccttccc cagcggccac
     1321 gccgcgtact cgaccgcctg ggtcgcggtc gcggtcgtcg tcgcgcgcgt cataccgggc
     1381 atcgcgagcc gcgccgcgct gatcggcggc gcgctcgccc tggccgcggc gatcgggctg
     1441 tcgcggatct acctgcgcgt gcactactgg tccgacgtcg ccggcggctg ggggctcggg
     1501 gcgggcctgt tcggcgcttg tgcggtcatc gggctcgtcg tcgcgcacgt gcgccacaat
     1561 ggcga
//
LOCUS       DATABV010000076        10151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2352711, whole genome shotgun sequence.
ACCESSION   DATABV010000076 DATABV010000000
VERSION     DATABV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352711"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VHF88_03130"
     CDS             <1..392
                     /locus_tag="VHF88_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2160792.1"
                     /translation="YADGVADVDLRAVLAFHRDHGAAASMTVVRPELQFGITRLGDDG
                     RVLGFEEKPRSEQWINGGFFCFEPAAFDYLSADSVLEREPLERLAADGALRAYRHSGF
                     WECMDTYKDAVQLNDLWDDGRAPWRTW"
     gene            429..1280
                     /locus_tag="VHF88_03135"
     CDS             429..1280
                     /locus_tag="VHF88_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX2160793.1"
                     /translation="MKSSATAALTLSAEIRSRFDEFSRSQKDVGQYIVDHLDEASFHT
                     AEELARRANTSSSTVVRFAQALGFEGFPELQSAARDEYRRAREGEQGTVDLANPPLFP
                     IDQTEFEAALAADHVNVEETARKLDREEVAHCVELISRADRLVLCGTDQMAFFASYLR
                     HLLMLLDVRCEVVASPSQEGLAKLGRIDDRTVLVGFSAGRPHPLVVRAMKLARNRNAA
                     TISVSDATLSEVAKLADHKLFYSSNSPAFVRSHAALLSVVQALAYGVYAMDESAYSDR
                     IKAFKLK"
     gene            1300..2187
                     /gene="pdxS"
                     /locus_tag="VHF88_03140"
     CDS             1300..2187
                     /gene="pdxS"
                     /locus_tag="VHF88_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2160794.1"
                     /translation="MRQEGTFAVKAGLAEMLKGGVIMDVVTAEQARIAESAGAAAVMA
                     LERVPADIRVAGGVARMSDPTMIEAIQAAVTIPVMAKARIGHFVEAEVLQALEVDYVD
                     ESEVLTPADEANHIDKWGFKVPFVCGATNLGEALRRIAEGAAMIRSKGDAGTGNVVEA
                     VRHMRQIGGDIRRLGALDEAELPTAAKELQAPLPLVRDVAKAGRLPVVMFSAGGVATP
                     ADAALMMRLGAEGVFVGSGIFKSEDPERTAVAVVEATTHFEDAERIAAASRDLGAPMT
                     GIGMDAIAEQGGLLQERGW"
     gene            2184..2768
                     /gene="pdxT"
                     /locus_tag="VHF88_03145"
     CDS             2184..2768
                     /gene="pdxT"
                     /locus_tag="VHF88_03145"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004130658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX2160795.1"
                     /translation="MTARTVGVLALQGDFEAHRKVLEGLGANVREVRTPADLDGIDGL
                     VIPGGESTTMTLGIEREQLAEPLRELAARGAPVLGTCAGMIMLDREHLGVLDVTTERN
                     AFGRQVHSFEAQLDLAGVGDGVEAIFIRAPWAGEVGEGVEVLAAVDGHPVAVRQRDVL
                     AVSFHPELNGETRLHEWLLARIAEATTPTGGEAQ"
     gene            2765..3868
                     /locus_tag="VHF88_03150"
     CDS             2765..3868
                     /locus_tag="VHF88_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX2160796.1"
                     /translation="MKDSRVEALAQILVRYSTKVQKGDVCVIQGTTTGEPLIQAVYEE
                     VLRAGGLPVMQLTTEQAQAAFFELASDEQLDWVQPPAEWTAEHSDVRIVVMADANPRA
                     LSGVDPARQARAQRARRKIMETSMRRAAEGKHRWSLTLFPTHAYAGEAGMSLSAYEQF
                     FYDACLATDPDPVTAWQRQSDEVLRLADWIEGREEVHVTGPGTDITLGVAGRHWIPCV
                     GTHNMPDGEFFTGPVEDSVNGEIAFSLPASYGGREVSGVRFRFKDGKVIDASAERGED
                     FLIEVLDTDEGSRTLGELGIGTNYGITAGTKEILLDEKIGGTVHMAIGAAYPESGGTN
                     ESAVHWDMICDLRQGGAIVVDGVELQRDGKFTV"
     gene            3881..4633
                     /locus_tag="VHF88_03155"
     CDS             3881..4633
                     /locus_tag="VHF88_03155"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2160797.1"
                     /translation="MAGHSKWASIKHKKKATDAKRGALFTKLTRAITVAAREGGGDPD
                     GNPALVLAIQKAKDASMPKDNIERAIAKGSGADQDAEAFETVTYEGYGPGGVAVLVEA
                     LTDNRNRTSSDVRHAFTKHGGNLGEPGSVAWIFEKKGQIVVDAARYDEDDLMPAIEAG
                     AEDVAADGDVLEVVTGPTDLAAVRGALELAGVEIQSAEQVMRPTNRIEVDEGQAATLM
                     RLLDVLEESDDVQAVHANFDVDAEILERVAAG"
     gene            4673..5164
                     /gene="ruvC"
                     /locus_tag="VHF88_03160"
     CDS             4673..5164
                     /gene="ruvC"
                     /locus_tag="VHF88_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX2160798.1"
                     /translation="MVVVLGIDPGTANTGFGVVVARGPKLGALDGGTIDTAAGVPLER
                     RLVQIHERILELIAEHEPEAVAIEQLYFGQNVRTALAVGQARGAVLLAAGQAGLPCFS
                     YTPQQIKQAVCGSGAAAKDQVQRMVGALLELEEIPKPDHAADALAVAICHANGAPLRA
                     AVG"
     gene            5201..5776
                     /gene="ruvA"
                     /locus_tag="VHF88_03165"
     CDS             5201..5776
                     /gene="ruvA"
                     /locus_tag="VHF88_03165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX2160799.1"
                     /translation="MISSLRGEVLVRRPGEVVVEAAGVGYKLAVSSETLGKVPQVGGE
                     VLLHSHLVLRDDGIQLFGFASEIERELFLMLIGVQSVGPKVAQAVLSGGPPRELLGAI
                     ANGDAARFQAVPGIGKRTAERIIVELREKVAGAVADEIVITRASDDPQSIAREALLGL
                     GYTPPEADAMLDGTDGETAEELIAEALKAVR"
     gene            5773..6813
                     /gene="ruvB"
                     /locus_tag="VHF88_03170"
     CDS             5773..6813
                     /gene="ruvB"
                     /locus_tag="VHF88_03170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX2160800.1"
                     /translation="MSGIRTPGVERLQDPEIEGGAEEELDRSLRPQLLADFVGQEQVK
                     AQLGVFIEAARARGEALDHVLLAGPPGLGKTSLAQIVANELGVGLVATAAPALERKGD
                     VASYLSSLEPRSVFFVDEIHRLPRALEETFYPAMEDRRLPITVGQGAGARVVNLDLPP
                     FTLIGATTRAGLLTTPLRDRFGVAPRLELYDPVDLGRIVRRSAGILGVEIDSAGAEAI
                     ASRSRGTPRVANRLLKRVRDYAEVRGAGRIDAAVATEALELLDVDRAGLDRLDREILT
                     AICSKFGGGPVGLSTLAVAVSEEQDTIEDVYEPYLLQQGLIMRTPRGRVATPAAFAHL
                     GLEPPSEGARLF"
     gene            6837..7301
                     /locus_tag="VHF88_03175"
     CDS             6837..7301
                     /locus_tag="VHF88_03175"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2160801.1"
                     /translation="MPNFICPNCGNRSVSTERTAGFQSRARGCKECGFGFLFELLDDY
                     YPAPTAAFFLCDREGRLIGAGRGARELTGLGDLDVIGRPAHEVLGLQFENGSDHLATA
                     LEWGVRVLGKPVVVNAEGDRPETAVADIFPAYDDDGGLLLVLTPRQPGGNDD"
     gene            7303..>10151
                     /gene="secD"
                     /locus_tag="VHF88_03180"
     CDS             7303..>10151
                     /gene="secD"
                     /locus_tag="VHF88_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX2160802.1"
                     /translation="MTSRKRNLSIIGLVLVLLALSGYVITSKPTVLGLDLRGGVELVY
                     EGRPTPQVPEVTAQAIDDAIETIRKRTDALGVSEPEIQRAGENQISIGLPDVENADRA
                     IEQVGTTAQLQFYDWEPNILGNQGPDAPFAGRTSLYDAVEVAAEQKPRAEPTDIPPDG
                     VEEAVEQRFDGDRKQIQRYYDRRNDSHSGRYYLFGPDKDLIAGPDSTCKELLSDYEGV
                     ERGPERRPAQIPKGSECREELAAIPVGDHQGGAEESTATPSSGFPAGSEVLEVPRGVV
                     VVEATRAPNQPEDVQNYFVIEDDSELSGADIKNPEQNTDQRTNEPIVTMEFSDDGREA
                     FAAVTKRIAQRGSEIIVPPGTDREQTLQRFAITLDDEIVSLATIDYRENPEGIDGRTG
                     AQINGIGSLQETQDLAESLRIGALPIELKLVSQTQVSATLGQQALDQGLLAGGAGLVL
                     TLLFLITFYRVLGVVAAVTLVIYAIFLFALVKLIPITMTLPGIAGTILTLAVAADANI
                     VIFERIKEEARAGSSIPAAISNGYQKALRTIIDANVVTIGVAFILFTLATSGVKGFAF
                     TMGVGTIVSLFTAVLATSAILGAMSRTKLIGSRFALGVGKDDREGPRWHWDFVGKSKW
                     FFSGSGLILVVGALAIAGIGLKFGIDFESGTRMKTPVAQEASVEDIRDALAPIGYEDA
                     EIQEVDEPELGAHVFQISTETLSGPEVSEVRETLDGEFGVERADFSVNSIGPTFGAQV
                     ARTALIAVIASLLLISIYMALRFEAKFVVPVLIAIAHDILITGGVYALLGREVTTSTV
                     AALLTILGYSLYDTIIVFDRIRENVPRMPRATFSQIVNRSMSEVLTRSLATSFSTLMP
                     VGALMFFGGETLQDFAFALLVGVASGAYSSIFIAAPVLTLWKEREPVYVRRRKIILEE
                     NDGVVPPFPSGVIGDDDGAAGAPARRKPPRRAPVPT"
BASE COUNT         1562 a         3587 c         3546 g         1456 t
ORIGIN      
        1 cctacgcgga cggcgtcgcc gacgtcgacc tccgtgcggt gctcgccttc caccgcgatc
       61 acggcgccgc cgcgtcgatg accgtcgtgc ggcccgagct gcagttcggc atcacccgtc
      121 tcggagacga cggccgcgtc ctcggcttcg aggagaagcc gcgctccgag cagtggatca
      181 acggcggctt cttctgcttc gagccggccg ccttcgacta cctctcggcc gacagcgtcc
      241 tcgagcgcga gccgctcgag cggctcgccg ccgacggggc cctgcgcgcc taccgccaca
      301 gcgggttctg ggagtgcatg gacacctaca aggacgccgt ccagctcaac gacctctggg
      361 acgacgggcg ggcgccctgg cgcacctggt gagaaggaac tcccagacgg cgggcgaaac
      421 agaccgccgt gaagtcctca gccaccgccg cgctcactct gtccgccgag atccggtccc
      481 gcttcgacga gttctcgcgc tcgcagaagg acgtcggcca gtacatcgtc gaccacctcg
      541 acgaggcctc cttccacacc gcggaggagc tcgcgcgccg cgccaacacc tcttcgtcga
      601 ccgtcgtccg cttcgcccag gcgctcggct tcgagggctt ccccgagctc cagtcggccg
      661 cccgcgacga gtaccgccgg gcgcgtgagg gcgagcaggg gaccgtcgac ctcgccaacc
      721 cgccgctctt cccgatcgac cagaccgagt tcgaggccgc gctggccgcc gaccacgtca
      781 acgtcgagga gacagcgcgc aagctcgacc gcgaggaggt cgcccactgc gtcgagctga
      841 tctcgcgcgc cgaccggctc gtcctctgcg gcaccgacca gatggcgttc ttcgccagct
      901 acctgcgtca cctgctgatg ctgctcgacg tccgctgcga ggtagtcgcc agcccgagcc
      961 aggaggggct cgccaagctc ggccggatcg acgatcgcac cgtgctcgtc ggcttctcgg
     1021 cgggtcgccc gcatccgctg gtcgtgcgcg cgatgaagct cgcccgcaac cgcaacgcgg
     1081 cgacgatctc ggtctccgac gcgacgctct ccgaggtcgc caagctcgcc gaccacaagc
     1141 tcttctactc atcgaacagc cccgcgttcg tgcgttcgca cgcggcgctg ctcagcgtcg
     1201 tccaagcgct cgcctacggg gtgtacgcga tggatgagtc tgcctattcc gaccggatca
     1261 aggccttcaa gctgaagtag ctcaagtagt cttgccctca tgaggcaaga gggcaccttc
     1321 gctgtcaagg ccggcctggc agagatgctc aagggcggcg tgatcatgga cgtcgtgacc
     1381 gccgagcagg cgcggatcgc cgagtccgcc ggtgctgccg ccgtgatggc gctggagcgc
     1441 gtgccggccg acatccgcgt cgccggcggg gtcgcgcgga tgtccgaccc gacgatgatc
     1501 gaggcgatcc aggcggccgt cacgatcccc gtgatggcca aggcccggat cggccacttc
     1561 gtcgaggccg aggtgctgca ggcgctcgag gtcgactacg tcgacgagtc cgaggttctg
     1621 acgccggccg acgaggccaa ccacatcgac aagtggggct tcaaggtccc gttcgtctgc
     1681 ggcgcgacga acctcggcga ggcgctgcga cggatcgccg agggcgccgc gatgatccgc
     1741 agcaagggcg atgccggcac cggcaacgtc gtcgaggccg tccgccacat gcgtcagatc
     1801 ggcggcgaca tccgccgtct cggagcgctc gacgaggccg agctgccgac ggcggccaag
     1861 gagctccagg cgccgctgcc gctcgtccgc gacgtagcga aggctggccg gctgccggtc
     1921 gtgatgttct ccgccggcgg ggtcgccaca ccggccgacg ccgcgctgat gatgcggctc
     1981 ggggcggagg gcgtcttcgt cggctcgggc atattcaagt ccgaggaccc cgagcgcacg
     2041 gccgtcgccg tggtcgaggc gaccacccac ttcgaggacg cggagcggat cgccgccgcg
     2101 tcgcgcgacc tcggcgcgcc gatgaccggc atcggcatgg acgcgatcgc cgagcagggc
     2161 ggcctgctgc aagagcgcgg ttggtgaccg cccggaccgt cggcgtactg gcgctgcagg
     2221 gcgacttcga ggcccaccgc aaggtgctcg agggactggg cgcaaatgtt cgtgaggtgc
     2281 gcacgccggc cgacctcgac ggcatcgacg gtcttgtgat cccgggcggc gagtcgacga
     2341 cgatgacgct cgggatcgag cgcgagcagc tcgccgagcc gctgcgcgag ctggcggcgc
     2401 gcggcgcgcc ggtgctcggg acgtgtgcgg ggatgatcat gctcgaccgc gagcatctcg
     2461 gcgtgctcga cgtgaccacc gagcgcaacg ccttcggccg ccaggtccac agtttcgagg
     2521 cgcagctcga cctcgccggc gtcggcgacg gcgtcgaggc gatcttcatc cgtgccccgt
     2581 gggcgggcga ggtgggggag ggcgtcgagg tgctggccgc cgtcgacggc caccccgtcg
     2641 cggtccgcca gcgcgacgtg ctcgccgtgt ccttccatcc cgagctcaac ggcgagacgc
     2701 gcctgcacga gtggctgctc gcccgtatcg cagaggccac caccccgacc ggaggagaag
     2761 cccagtgaag gactcacgcg tggaggcgct cgcgcagatc ctcgtccgct actcgaccaa
     2821 ggtgcagaag ggcgacgtct gcgtcatcca ggggacgacg accggcgagc cgctgatcca
     2881 ggccgtctat gaggaggtcc tgcgcgccgg cggcctgccc gtcatgcagc tgaccaccga
     2941 gcaggctcag gccgcattct tcgagctggc gagcgacgag cagctcgact gggtccagcc
     3001 gccggccgag tggacggccg agcactccga cgtgcggatc gtcgtcatgg ccgacgcgaa
     3061 cccgcgcgcg ctgtccggcg tcgacccggc caggcaggca cgggcgcagc gggcgcggcg
     3121 caagatcatg gagacctcga tgcgccgcgc cgccgagggg aagcaccgct ggtcgctgac
     3181 gctgttcccg acccacgcct acgccggtga ggccgggatg tcgctctccg cctacgagca
     3241 gttcttctac gacgcctgcc tggcgaccga ccccgatccc gtcaccgcgt ggcagcggca
     3301 gtccgacgag gtgctgcggc tcgccgactg gatcgagggg cgcgaggagg tccacgtcac
     3361 cggccctggc accgacatca cgctcggcgt cgccgggcgc cactggatcc cctgcgtcgg
     3421 gacgcacaac atgcccgacg gcgagttctt caccggtccc gtcgaggact ctgtcaacgg
     3481 cgagatcgcg ttctcgctgc cggcctccta cggcggtcgc gaggtgtccg gcgtccgctt
     3541 ccgattcaag gacggcaagg tcatcgacgc gtccgccgag cgcggcgagg acttcctgat
     3601 cgaggtgctc gacaccgacg agggctcgcg cacgctcggc gagcttggca tcggcaccaa
     3661 ctacgggatc accgccggca ccaaggagat cctgctcgac gagaagatcg gcggcacggt
     3721 gcacatggcg atcggcgccg cctacccgga gtcgggcggc accaacgagt ccgccgtcca
     3781 ctgggacatg atctgcgacc tccgccaggg cggcgcgatc gtcgtcgacg gcgtcgagct
     3841 gcagcgcgac ggcaaattca ccgtttgaga ggattggggc atggccggcc actccaaatg
     3901 ggcgtcgatc aagcacaaga agaaggcgac cgacgccaag cgcggcgcgc tcttcacgaa
     3961 gctgacgcgg gcgatcacgg tcgccgcgcg cgagggcggg ggtgacccag acggcaaccc
     4021 agcactcgtg ctcgcgatcc agaaggccaa ggacgcgagc atgcccaagg acaacatcga
     4081 gcgcgcgatc gccaagggca gcggcgccga ccaggacgcc gaggcgttcg agacggtcac
     4141 ctacgagggc tacggccccg gcggcgtcgc ggtgctcgtc gaggcgctga ccgacaaccg
     4201 caaccgcacc agttccgacg tccgccacgc gttcaccaag cacggcggca acctcggcga
     4261 gccgggctcg gtcgcctgga tcttcgagaa gaagggccag atcgtggtcg acgccgcccg
     4321 ctacgacgag gacgacctga tgcccgccat cgaggccggc gccgaggacg tcgcggcgga
     4381 cggcgacgtg ctcgaggttg tgaccggtcc gacggacctc gcagcggtcc gcggcgcgct
     4441 cgagctggca ggcgtcgaga tccagtcggc tgagcaggtg atgcgcccga ccaaccggat
     4501 cgaggtcgac gagggccagg ccgcgacgct gatgcgcctg ctcgacgtgc tcgaggagag
     4561 cgacgacgtc caggccgtcc acgccaactt cgacgtcgac gccgagatcc tggagcgcgt
     4621 agccgcgggc tgatcgaggg aggcgtccgc gcctccgcga agaatcaggc ggatggtggt
     4681 cgtcctcggc atcgatcccg gcaccgcgaa caccggtttc ggcgtggtgg tcgcgcgcgg
     4741 gccgaagctc ggggcgctcg acggggggac gatcgacacc gcggcgggag tgccgctcga
     4801 gcggcgcctg gttcagatcc acgagcgcat cctcgagctg atcgccgagc acgagcccga
     4861 ggcggtagcg atcgagcagc tctacttcgg gcagaacgtc cgcacggcgc tcgcggtcgg
     4921 ccaggcgcgc ggcgcggtgc tgctggcggc ggggcaggcc ggcctgccgt gcttctccta
     4981 cacgccgcag cagatcaagc aggcggtctg cgggtccggc gccgccgcca aggatcaggt
     5041 gcagcggatg gtcggtgccc tgctcgagct cgaggagatc cccaagcccg accacgccgc
     5101 cgatgcgctc gccgtcgcga tctgccatgc caatggagcg ccgctgcgcg ccgccgtcgg
     5161 ctgagggagg cgtccggccg gcggcgcaga atcagcgact gtgatctcgt cgctgagagg
     5221 agaggtgctg gtccgccgtc ccggcgaggt ggtcgtcgag gcggccggcg tgggatacaa
     5281 gcttgcggta tcgagcgaga cgctcggcaa ggtgccgcag gtcggcggcg aggtgctgct
     5341 gcactcccac ctcgtcctac gcgacgacgg cattcagctg ttcggcttcg ccagcgagat
     5401 cgagcgcgag ctgttcctga tgctgatcgg cgtccagagc gtcgggccga aggtcgccca
     5461 ggcggttctc tccggaggcc cgccgcgcga gctgctcggc gcgatcgcca acggcgacgc
     5521 ggcgcgcttc caggcggtcc ccgggatcgg caagcggacc gccgagcgga tcatcgtcga
     5581 gctgcgcgag aaggtggccg gcgcggtcgc cgacgagatc gtgatcacgc gcgcgtccga
     5641 cgacccgcag agcatcgccc gcgaggcgct gctcgggctc gggtacacgc cgccggaggc
     5701 cgacgcgatg ctcgacggga ccgacggcga gacggccgag gagctgatcg ccgaggcgct
     5761 caaggcggtc cgatgagcgg gatccgcaca cctggcgtcg agcgcctgca ggatccggag
     5821 atcgagggtg gggcggagga agagctcgat cgctcgctgc ggccgcagct gctggccgac
     5881 ttcgtaggcc aggagcaggt caaggctcag ctcggcgtct tcatcgaagc cgctcgcgcc
     5941 cgcggcgagg cgctcgacca cgtgctgctc gccggtccgc ccggcctcgg caagacctcg
     6001 ctggcccaga tcgtcgcgaa cgagctcggc gtcggcctcg tcgcgaccgc cgcgccggcg
     6061 ctcgagcgca agggcgacgt cgcatcgtac ctctcgtcgc tcgagccgcg ctcggtcttc
     6121 ttcgtcgacg agatccaccg gctgccgcgc gcgctcgagg agaccttcta cccggcgatg
     6181 gaggatcgga ggctgccgat caccgtcggc cagggggccg gtgcccgcgt cgtcaacctc
     6241 gacctgccgc ccttcacgct gatcggcgcc acgacccgcg ccgggttgct gaccacgccg
     6301 ctgcgcgacc gcttcggcgt ggcgccgcgg ctcgagctct acgacccggt cgatctcggc
     6361 cggatcgtgc gtcgctcggc gggcatcctc ggcgtcgaga tcgacagcgc cggggcggag
     6421 gcgatcgcga gccgctcgcg cggcacgccg cgcgtcgcca accgcctgct caagcgcgtg
     6481 cgcgactacg ccgaggtccg cggcgccggc aggatcgacg cggctgtcgc gaccgaggcg
     6541 ctcgagctgc tcgacgtcga ccgcgccggg ctcgaccggc tcgatcgcga gatcctgacg
     6601 gcgatctgct cgaagttcgg cggcgggccg gttggccttt cgacgctcgc cgtcgccgtc
     6661 agcgaggagc aggacacgat cgaggacgtc tacgagccct acctgctgca gcaggggctg
     6721 atcatgcgca cgccgcgcgg ccgggtggcg accccggcgg ccttcgcgca cctggggctg
     6781 gagccgccca gcgaaggggc tcgcctcttc tgaggcgccg ctaatctccg cctcccgtgc
     6841 cgaacttcat ctgtccgaac tgcggtaacc gcagcgtctc gaccgagcgc accgccggct
     6901 tccagagccg agcgcgcggt tgcaaggagt gcggcttcgg cttcctcttc gagctcctcg
     6961 acgactacta cccggcgccg accgcggcct tcttcctctg cgaccgggag gggcgcctga
     7021 tcggcgccgg acgtggcgca cgcgagctga ccggtctcgg cgatctcgac gtgatcggcc
     7081 gaccggcgca cgaggttctc gggctccagt tcgaaaacgg cagtgaccac ctcgccacag
     7141 cacttgagtg gggcgttagg gttctcggaa agcctgtcgt ggtcaatgcg gagggagacc
     7201 ggccagagac cgctgtggct gacatcttcc ccgcttacga cgatgatggt gggctgctgc
     7261 tggtgctgac gccgcgccag cccggcggca acgacgactg acatgacttc acgcaagcgc
     7321 aacctctcga tcatcggcct cgtgctggtc ctgctggcgc tgtccggcta tgtgatcacc
     7381 agcaagccga cggtgctagg gctcgacctg cgcggcggcg tcgagctcgt ctatgagggg
     7441 cgcccgacgc cgcaggtccc ggaggtcacc gcacaggcga tcgacgacgc gatcgagacg
     7501 atccgcaagc gcaccgacgc gctcggcgtc tcggagccgg agatccagcg ggccggcgag
     7561 aaccagatct cgatcggctt gcctgacgtc gagaacgccg atcgggcgat cgagcaggtc
     7621 gggaccaccg cgcagctgca gttctacgac tgggagccga acatcctcgg caaccagggg
     7681 cccgacgcgc cgttcgccgg gcgcacgtcg ctctacgacg cggtcgaggt cgcagccgag
     7741 cagaagccgc gtgcggagcc gaccgacatc ccgcccgacg gggtcgagga ggcggtcgag
     7801 cagcgcttcg acggcgaccg gaagcagatc cagcgctact acgaccggcg caacgacagc
     7861 cacagcggcc gctactacct gttcggtccg gacaaggacc tgatcgccgg ccccgactcc
     7921 acctgcaagg agctgctctc cgactacgag ggcgtcgagc gcggccccga gcgacgaccc
     7981 gcgcagatcc ccaagggcag cgagtgccgc gaggagctcg cggcgatccc ggtcggcgac
     8041 caccagggcg gcgccgagga gtcgacggcg accccgagca gcggcttccc agccggatcc
     8101 gaggtcctcg aggtgccgcg tggcgtcgtc gtggtagagg cgacgcgtgc gcccaaccag
     8161 cccgaggacg tccagaacta cttcgtcatc gaggacgact cggagctctc cggcgccgac
     8221 atcaagaacc ctgagcagaa caccgatcag cgcaccaacg agccgatcgt cacgatggag
     8281 ttcagcgacg acggccgcga ggccttcgcc gcggtcacca agcggatcgc ccagcgcggc
     8341 tcggagatca tcgtcccgcc cggcaccgac cgcgagcaga cgctgcagcg cttcgcgatc
     8401 acgctcgatg acgagatcgt ctcgctggcg acgatcgact accgcgagaa cccggagggc
     8461 atcgacgggc gcaccggcgc ccaaatcaac gggatcggga gcctgcagga gacgcaggac
     8521 ctcgctgaga gcctgcgcat cggcgcgctg ccgattgagc tgaagctcgt ctcgcagacc
     8581 caggtctcgg cgacgctcgg tcagcaggcg ctcgatcagg ggctcttggc cggcggcgcg
     8641 ggactggtgc tgacgctgct cttcctgatc acgttctacc gcgtcctcgg cgtggtagcg
     8701 gccgtcacgc tggtcatcta cgcgatcttc ctgttcgcgc tggtcaagct gatcccaatc
     8761 acgatgacgc tgccggggat cgccggcacg atcctcacgc tcgcggtcgc ggctgacgcg
     8821 aacatcgtca tcttcgagcg aatcaaggag gaggcgcgcg cgggctcgtc gatacccgcg
     8881 gcgatctcca acggctacca gaaggcgctg cgcacgatca tcgacgcgaa cgtcgtcacg
     8941 atcggcgtcg cgttcatcct ctttacgctc gccacctcag gcgtcaaggg cttcgccttc
     9001 acgatgggcg tcggcacgat cgtctcgctg ttcacggcgg tgctcgctac ctcggcgatc
     9061 ctcggcgcca tgagccgcac gaagctgatc ggctcgcgct tcgcgctcgg cgtcggcaag
     9121 gacgaccgcg agggcccgcg ctggcactgg gacttcgtcg gcaagtcgaa gtggttcttc
     9181 tccggctccg ggttgatcct cgtcgtcggc gcgctcgcga tcgccggcat cgggctgaag
     9241 ttcggcatcg acttcgagtc cggcacccgg atgaagacgc cggttgccca ggaggcgagc
     9301 gtcgaggaca tccgcgacgc gctcgcgccg atcggctacg aggacgccga gatccaggag
     9361 gtcgacgagc ccgagctggg cgcgcatgtc ttccagatct ccaccgagac gctcagcggt
     9421 cccgaggtca gcgaggtgcg cgagacgctc gacggcgagt tcggagtcga gcgggcggac
     9481 ttctcggtca actcgatcgg gccgaccttc ggcgcccagg tcgcgcgtac cgcgctgatc
     9541 gccgtcatcg catcgctgct gctgatctcg atctacatgg cgctgcgctt cgaggcgaag
     9601 ttcgtcgtcc cggtcctgat cgcgatcgcc cacgacatcc tgatcaccgg cggcgtgtac
     9661 gccctgctcg gccgggaggt gaccacgtcg accgtcgcgg cgttgctgac gatcttgggc
     9721 tactccctct acgacacgat catcgtgttc gaccgcatcc gcgagaacgt cccacggatg
     9781 ccgcgcgcga ccttctccca gatcgtcaac cgatcgatgt ccgaggtgct gacgcgctcg
     9841 ctcgcgacca gcttctcgac gttgatgccg gttggcgcgc tgatgttctt cggcggcgag
     9901 acgctgcagg acttcgcctt cgcgctgctg gtcggcgtcg cctccggcgc ctactcgtcg
     9961 atcttcatcg cggcgcccgt gctgacgctc tggaaggagc gtgagcccgt ctacgtgcgc
    10021 cggcgcaaga tcatcctcga ggagaacgac ggcgtcgtcc cgccgttccc gtcgggcgtg
    10081 atcggcgacg acgacggtgc cgcgggcgct cccgcgcgcc gcaagccgcc gcgccgcgcg
    10141 cccgtgccga c
//
LOCUS       DATABV010000077         5866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_747090, whole genome shotgun sequence.
ACCESSION   DATABV010000077 DATABV010000000
VERSION     DATABV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747090"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2086)
                     /locus_tag="VHF88_03185"
     CDS             complement(<1..2086)
                     /locus_tag="VHF88_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2160803.1"
                     /translation="MSTRFASAVVRLRWVVLAFWIAAAAVVVTQLPTIREAQVGALGD
                     LVPRNSDAVDAELRSSELFRFPLLSRTIVVQRDADGLGVAEEARIAARALAINRGQDR
                     PLRRVAGALPLTNAVGHSDLARERSTTAITYLFFQPEVGREDRQILAERLVERRIEPR
                     FDGFAGVTGAIAARSQQSELISESLPLVELATVLLVALVVALHFRALGAPLVTLTAVG
                     ISYVVSIRLIAWIGQKLGISVPSEVEPVIVVLLFGVVTDYSIFFLSRVRSRIADGEDA
                     RTATVRGTAELLPIIVTAGLTVVAASASLVAAKLGFLEAFGPGVALAVLIGLLVAITL
                     IPALLAIGGSAVFWPRRPGVEVSSAEAAEEKPGAADRRPRRSRALEIATRRPRLVALA
                     CGAFLLVAASGLTTIDLGNPLIRGLPADADAREGYRQASQGFAPGILSPTVVIVEGDG
                     VAGRRRALSRFQALLAQRRGVAEVVGPADQPAERAFGAVLSETGDAARFFVVLGTDPL
                     GGRAVQRLRALRARLPSLLREAGLNGATVSVAGDTALVTETIDKTVDDLARVAPVAIA
                     VLLIILAGFLRALVAPLYLVGASVLALAASLGLTGYVFQVLLGHGEITYFVPFAAAVL
                     LVSLGSDYNVFLVGRIWDEARERPLREAVRVAGARAATPITIAGLVLAGSFALLALVP
                     IRPFQELAFAMAA"
     gene            complement(2094..2510)
                     /locus_tag="VHF88_03190"
     CDS             complement(2094..2510)
                     /locus_tag="VHF88_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160804.1"
                     /translation="MRRPRHPFLDLLLALWVVGWLALAYQVASEVRGLRDLSETVTET
                     GVAVRESGEALGSLSDLPLVGDRVAEPAQRIEAAGQSAADSGRSTRDSIDTLSILLAI
                     AIGGIPPIAVLGIYLQLRREQTLVDEARGRAGRSAG"
     gene            complement(2587..3321)
                     /locus_tag="VHF88_03195"
     CDS             complement(2587..3321)
                     /locus_tag="VHF88_03195"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2160805.1"
                     /translation="MKLIGAGLPRTATTTQLVALEMLGLPCYHMRDMMADPATSIPQW
                     RKAFEGNGDWDELFAGKESIVDWPGSYHWRELIDVYPDAKVLLSVRSAESWVESMHNT
                     IAAIWFGDNLMHHLAMAQYHVDPLYASWLDLLNDMWTKADIMVGSRGDAEEMAAGMER
                     WNQEVIDTVPSDRLLVWNPADGWEPLCELLDVPVPSEPLPNVNDTENFQKNLIMRPAI
                     EAINRWWEQNSGPGDQVAERPAPAAT"
     gene            complement(3434..>5866)
                     /locus_tag="VHF88_03200"
     CDS             complement(3434..>5866)
                     /locus_tag="VHF88_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160806.1"
                     /translation="RAAAQPAATTAPPAAPVGLPAASTPIGMLPAPASLETVTPSARS
                     RPVATPPPRRAAPGCVTEIVSLDVAPNASALGAVFDSRAQQDGKTGIRVGTSFYPLPA
                     GRAALLVLDAETLEDLSFTTVPTGLPVQAWAIEQTMRYQVSGRDVLMVLAGMPGCCAD
                     DPAYPSTGFTVVQRPGQSAGTIAQNRGLVVAAGGAPGQLTGYLQQSVPLDGAGPSYRL
                     VSPDRVAFDTQASATPTTNTMRVGEATYPGALPAGASAGFHVLVIDAAQRPQLGTPTV
                     FATNTGDAATDRQQEQSMNQLATAALKQPGTTTLVQSIGHPRPSSNVAWQLAVTLEQL
                     GGTPWMFLGLDGSGDYALVGNPPPPGTFVNPVAAAEASSQWQGGGHGDLQGVLRRRGD
                     RALYAPLADPVGTPDYGLAEVVYQPPTAWPQTDTPGKIAATSYLATQLGLLSGPGSCY
                     QPPKPDFRSSYCNESIDVDALDRQLDRIAYPSGTSDFSKPEFDAVVAQLDTELDYVGD
                     VRALVTSLQKPFGTAEIAPAVDSSEIAGQLVAALPPKPGSALSGDLELASSILYVGAE
                     VPVVGEALGPIASVLDLASVLTEQDAQLSPDWDIQTTADKMGGEIEGRLQAMSGALGL
                     FQDVLVSDYGKLSTAATDAESAWGVNGAGIDEQISTLELGVKQWLWTAILPSAFDLIK
                     VPGVPLDQPGQAWCVYSRAPAEWRPWQKAPPASVFFPFDGMNGTQQTASAMFGMLSGK
                     FSDRSSETVTPGLARLLFASPDTSVGPGQPTPAGLVRPWLFERARWTVRQPKMVQPDS
                     GLVPGYCGVGDT"
BASE COUNT          862 a         2106 c         2130 g          768 t
ORIGIN      
        1 ctgccgccat tgcgaaggcg agctcttgga acgggcggat cggaaccaac gcgagcaggg
       61 cgaacgatcc cgcgaggacg agcccggcga tcgttatcgg ggtcgccgcg cgcgctcccg
      121 cgaccctgac ggcctcgcgc aacggccgct cgcgcgcctc gtcccagatc cgcccgacga
      181 ggaagacgtt gtagtcggac ccgagcgaca cgagcagaac cgccgcggcg aacggcacga
      241 agtaggtgat ctcgccgtgg ccgagcagga cttggaacac gtagccggtc agccccagcg
      301 aggcggcgag cgcgagcacg ctcgcgccga cgaggtacag cggcgcgacc agcgcacgca
      361 agaagccggc gaggatgatc agcaggacgg cgatcgcgac cggcgcgacg cgagcgagat
      421 cgtcgaccgt cttgtcgatc gtctcggtca ccagtgcggt gtctccggcc acggagacgg
      481 tcgcgccgtt caagccggcc tcgcgcaaga gcgacggcag ccgggcgcgc agcgcacgca
      541 accgctgcac cgcgcgtccg ccgagcgggt cggtaccaag gacgacgaag aagcgggccg
      601 cgtcgcccgt ctcggacaga acggcgccga acgcccgctc ggccggctgg tcggcgggcc
      661 cgacgacctc ggccacgccg cgacgctgcg caagcagggc ctggaaccgg ctcagcgcac
      721 gtcgtcggcc cgcgacgccg tcgccctcca cgatcacgac ggtcggcgag aggattcccg
      781 gcgcgaaccc ttggcttgcc tgtcggtagc cctcgcgcgc gtcggcgtcc gcggggagcc
      841 cgcggatgag cggattgccg aggtcgatcg tggtgagccc ggacgcggcg accagcagga
      901 aggcgccaca ggcgagcgcg accagtcgtg ggcggcgagt cgcgatctcc agcgcccgcg
      961 accgccgcgg ccggcggtcc gccgcgcccg gcttctcctc cgcggcctcc gcgctcgaca
     1021 cctcgacgcc ggggcgccgc ggccagaaca cggcgctgcc gccgattgcg agcagcgctg
     1081 ggatcagcgt tatcgccacg agcaggccga tgagcaccgc cagcgccact cccggaccga
     1141 acgcctcgag gaacccgagc ttggcggcga ccagcgacgc gctcgccgcg acgacggtca
     1201 gtccggccgt cacgatgatc ggaagcagct cggcggtccc gcgcacggtc gcggtgcgcg
     1261 cgtcctcgcc gtcggcgatc cggctgcgga cgcgcgacag gaagaagatc gagtagtcgg
     1321 tcaccacgcc gaacagcagc accacgatca ccggctcgac ctcgctcggc accgagatcc
     1381 ccagcttctg gccgatccag gcgatcagcc ggatcgagac gacgtaggag atcccgacag
     1441 cggtgagcgt cacgagcggc gccccgagcg cgcggaagtg cagcgccacg acgagggcta
     1501 ccagcagcac ggtcgcgagc tcgacgagcg gcagcgactc cgagatcagc tccgactgct
     1561 gactccgcgc ggcgatcgcg ccggtgacgc ccgcgaaacc gtcgaaccgg ggctcgatcc
     1621 gccgctcgac aaggcgctcg gccaggatct gacggtcctc gcgtccgacc tcgggctgga
     1681 agaagaggta ggtgatcgcc gtcgtcgacc gctcacgcgc gaggtccgag tggccgacgg
     1741 cgttcgtgag cggcaacgct ccggcgacgc gccgcaacgg acggtcctgg ccgcggttga
     1801 tcgccagggc gcgagccgcg atgcgcgcct cctccgcgac cccgagccca tcggcgtcgc
     1861 gctggacgac gatcgtccgg ctcaggagcg gaaagcggaa gagctcgctg gaccgcagct
     1921 ccgcgtcgac ggcgtcggag ttccgtggga cgaggtctcc cagcgccccg acctgcgcct
     1981 cgcggatcgt cggcagctgc gtgacgacga cggcggcggc cgcgatccag aacgcgagga
     2041 cgacccagcg gagccggacg accgcggacg cgaaccgcgt cgacacctcc cggtcagccc
     2101 gccgatcggc ccgcgcgacc gcgggcctcg tcgacgagcg tctgctccct gcgaagctgc
     2161 agatagatcc cgaggaccgc gatcggcggg atgccgccga tcgcgatcgc gagcaggatg
     2221 ctcagcgtgt cgatgctgtc gcgcgtcgag cggccgctgt ccgccgcgct ctggccggcc
     2281 gcctcgatcc gctgcgccgg ctcggccact cgatctccta cgagcggcag atccgagagg
     2341 cttcccaatg cctctcccga ctcgcgaacc gccaccccgg tctcggtgac ggtctcgctc
     2401 aggtcccgca ggccgcgcac ctcgctggcg acctgatacg cgagtgccag ccacccgacg
     2461 acccagagcg cgagcagcag atcgagaaac gggtggcgcg ggcgacgcat gccgaagaca
     2521 tgcccgcgcc cgcggcccgg caaccgcgcc ccggcgctct ccgccggcgg agccgtccag
     2581 gcgcggctac gtagccgccg gcgccggccg ctccgcgacc tggtccccag gccccgagtt
     2641 ctgctcccac cacctgttga tggcctcgat cgcaggacgc atgatcaggt tcttctgaaa
     2701 gttctcggtg tcgttgacgt tcggcagcgg ctcgctcggg acagggacgt cgagcagctc
     2761 gcagagcggc tcccagccgt ccgcggggtt ccagaccagc agacggtcgg agggaaccgt
     2821 gtcgatgacc tcctggttcc agcgctccat cccggccgcc atctcctcgg cgtcgcccct
     2881 gctgccgacc atgatgtccg ccttcgtcca catgtcgttg aggaggtcga gccacgaggc
     2941 atagagcggg tcgacgtggt actgcgccat cgccaggtgg tgcatgaggt tgtcgccgaa
     3001 ccagatcgca gcgatcgtgt tgtgcatgct ctcgacccag ctctcggcgg agcggacgct
     3061 caggagcacc ttggcgtctg ggtagacgtc aatcagctcg cgccagtggt acgacccggg
     3121 ccagtcgacg atcgactcct tgccggcgaa gagctcgtcc cagtccccgt tgccctcgaa
     3181 ggccttgcgc cactgcggga tgctggtcgc ggggtcggcc atcatgtcgc gcatgtggta
     3241 gcagggcagc ccgagcatct cgagcgcaac cagctgggtt gtcgttgcag tccgcggcaa
     3301 gcccgcgccg atcagcttca tgcgtgtctc ctcctggtgg gtgggaagcc gccgcatcct
     3361 atgacgctgg cgacgacccc agggggccaa ggcaggggtc ccgagcgatc ccgccgatct
     3421 gccgcgctcc tggctaggtg tcgcccacgc cgcagtagcc gggcacgagc cccgagtcgg
     3481 gctggaccat cttcggctgc cggaccgtcc acctggcgcg ctcgaacagc cacgggcgga
     3541 cgagcccagc cggcgtcggc tggccgggac cgaccgaggt gtcaggcgag gcgaacagca
     3601 gcctggcgag gcccggggtc accgtctcgg agctcctgtc ggagaacttg ccgctcagca
     3661 tcccgaacat cgccgaagcc gtctgctgcg ttccgttcat gccgtcgaac gggaagaaga
     3721 ccgacgccgg cggcgccttc tgccacgggc gccactccgc cggcgcgcgg ctgtagacgc
     3781 accaagcctg ccccggctgg tcgagcggca cgccggggac cttgatgagg tcgaaggccg
     3841 acggcaggat cgccgtccac agccactgct tgacgcccag ctcgagcgtc gagatctgct
     3901 cgtcgatccc ggcgccgttc accccccacg cgctctcggc gtcggtggcc gccgtcgaga
     3961 gcttgccgta gtcggacacg agcacgtcct ggaagaggcc gagggcgccc gacatcgcct
     4021 gcagccgccc ctcgatctcg cctcccatct tgtcggccgt cgtctggatg tcccagtccg
     4081 gcgacagctg ggcgtcctgc tcggtgagca ccgacgccag gtcgagcacc gacgcgatcg
     4141 gcccgagcgc ctcgccaacg accggcacct cggcgcccac gtagaggatc gacgacgcga
     4201 gctcgaggtc gccgctcagc gcgcttccgg gcttcggcgg cagcgcggcg acgagctgtc
     4261 cggcgatctc ggacgagtcg acggccggcg cgatctcggc cgtcccgaat ggcttctgga
     4321 gcgacgtgac cagcgcccgg acgtcgccga cgtagtcgag ctcggtgtcg agctgcgcga
     4381 cgacggcgtc gaactccggc ttgctgaagt ccgacgtgcc ggacgggtag gcgatgcggt
     4441 cgagctggcg gtcgagcgca tccacgtcga tgctctcgtt gcagtaggac gagcggaagt
     4501 ccggcttcgg cggctgatag caggagccgg ggcccgacag gagcccgagc tgcgtcgcga
     4561 ggtaggaggt cgcggcgatc ttgccgggcg tgtcggtctg cggccaggcg gtcggcggct
     4621 ggtagacgac ctcggccagg ccgtagtcgg gggtgccgac cgggtctgcg aggggcgcgt
     4681 agagcgcccg gtcgccgcgc cggcgcagga cgccctggag gtcgccgtgg ccgccgccct
     4741 gccactgcga gctcgcctcg gcggccgcga cggggttcac gaaggtgccg ggcggcggcg
     4801 ggttgccgac gagcgcgtag tcgccgctgc cgtcgaggcc gaggaacatc cacggagtgc
     4861 cgccgagctg ctcgagcgtc accgccagct gccaggcgac attcgagctc gggcgcgggt
     4921 ggccgatgct ctggacgagg gtggtcgtcc ccggctgctt cagggccgca gtcgccagct
     4981 ggttcatcga ctgctcctgc tggcgatcgg tggccgcgtc gccggtgttg gtcgcgaaga
     5041 cggtcggggt gccgagctgc ggccgttggg ccgcgtcgat gacgagcacg tggaagcccg
     5101 cgctggcgcc cgcgggcaac gcgccggggt aggtcgcctc gccgacgcgc atcgtgttcg
     5161 tggtcggcgt cgcggacgcc tgcgtgtcga aggcgacacg atccggcgat acgagccggt
     5221 acgaggggcc ggcgccgtcg agcggcacgc tctgctggag gtagccggtc agctgtcccg
     5281 gcgcgccgcc ggcggcgacg acgaggcccc tgttctgcgc gatcgtgccg gcgctctggc
     5341 ccggccgctg gacgaccgtg aagccggtgc tcggatacgc ggggtcgtcc gcgcagcagc
     5401 caggcattcc ggccagcacc atcagcacgt cgcgtcccga cacctggtag cgcatcgtct
     5461 gctcgatcgc ccacgcctgg accggcagac cggtgggcac cgtggtgaac gacaggtcct
     5521 cgagcgtctc cgcgtcgagg acgagcagcg ccgccctccc ggcgggaagc gggtagaagc
     5581 tggtgccgac gcggattccg gtcttgccgt cctgctgggc gcgcgagtcg aacaccgcgc
     5641 cgagcgccga cgcgttcggc gccacgtcga gcgagacgat ctcggtgacg cagccgggag
     5701 ccgccctgcg cggcgggggc gtcgcgacgg gccggctgcg ggccgacggc gtgacggtct
     5761 ccagactcgc cggcgccggc agcatgccga tcggcgtcga ggcggcgggc aggccgacgg
     5821 gggcggcggg cggagccgtc gtcgccgccg gctgcgccgc ggcgcg
//
LOCUS       DATABV010000078         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2514235, whole genome shotgun sequence.
ACCESSION   DATABV010000078 DATABV010000000
VERSION     DATABV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2514235"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VHF88_03205"
     CDS             complement(<1..1349)
                     /locus_tag="VHF88_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2160807.1"
                     /translation="MTSVALKSLASRKLRAFSTVLAVLLGVALVAGTYILTDTINRSF
                     DDIFETALSGTDVVVVPKEIVAQETSEPPPFSASLLDRVERVPGVAKAEGGIFALVRI
                     TDAEGEPLGQGFAPQFAFSAASKPFDTLTYVEGRAPSSAREAAIDESNADRAGIEVGD
                     TIGVAADGPLKRFRVVGINKLGDTETGGSSSVTLTLPEAQRITGREGKFDQISVAADP
                     GVSPTELERRVARVVPSTVQVETGQENAQRETDEIGSDLGFLKIALLVFAGISVVVGG
                     FQIFNTFSITVAQRIREFGLLRTLGASRAQILTAVLLEALVIGLLGSLLGLVAGIGFA
                     AGINALFEAIGIDLPNTGLVFATRTVIVALAVGLGITLAAALTPALRATRVTPMAALL
                     EAALPERRRRGRITAVVAALLGLGGLAMLLIGLFGDIDSAGSAAGLMGAGSAAILFAT
                     "
     gene            complement(1354..>1908)
                     /locus_tag="VHF88_03210"
     CDS             complement(1354..>1908)
                     /locus_tag="VHF88_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2160808.1"
                     /translation="YGFMGGIQRFTPGGGYGKQLKDDYRRYYGAVVGFAFQFFNLLPV
                     LTAEENIRLPLSIAGRKPDAEWLDHLIEVVGLGDRRTHRPAELSGGQQQRVAVARALV
                     SKPAVVFADEPTGNLDSKASDEVLSLLRHSVDELGQTVVMVTHEAHAAAYADRLVVLR
                     DGRVAHDGVAGGEDDVLELMKAVA"
BASE COUNT          286 a          688 c          681 g          253 t
ORIGIN      
        1 gtcgcgaaca ggatcgccgc cgacccggcg cccatcagcc cggccgccga gccggccgag
       61 tcgatgtcgc cgaagaggcc gatcagaagc atcgccagac cgccgaggcc gagcagcgcc
      121 gcgacgaccg ccgtgatcct cccccgccgg cgacgctcgg gcagcgcggc ctcgagcagc
      181 gccgccatcg gcgtcacgcg cgtcgcgcgc agcgccggcg tcagcgcggc cgccagcgtg
      241 atgcccaggc cgaccgcgag cgcgacgatc accgtgcgcg tcgcgaacac gagcccggtg
      301 ttcggcaggt cgatcccgat cgcctcgaag agcgcgttga tgccggcagc gaacccgatg
      361 ccggcgacga gcccgagcag cgatccgagc agcccgatga cgagcgcctc gagcaggacc
      421 gcggtgagaa tctgggcgcg cgaggcgccc agcgtgcgca gcagcccgaa ctcgcgtatc
      481 cgctgcgcca ccgtaatcga gaaggtgttg aagatctgga agccgcccac gacgaccgag
      541 atcccggcga agacgagcag cgcgatcttc aggaagccga ggtcgctgcc gatctcgtcg
      601 gtctcccgct gggcgttctc ctgcccggtc tcgacctgga ccgtcgacgg gacgacgcgc
      661 gcgacacggc gctcgagctc ggtcggcgag acgcccgggt cggcggcgac ggagatctga
      721 tcgaacttgc cctcgcggcc cgtgatccgc tgcgcctcgg gcagcgtcag cgtcaccgag
      781 ctcgagccgc cggtctcggt gtctccgagc ttgttgatgc cgacgacgcg gaagcgcttc
      841 agcggcccgt cggcggcgac gccgatcgtg tcgccgacct cgatcccggc gcggtcggcg
      901 ttcgactcgt cgatcgcggc ctcgcgcgcg ctgctcggcg cgcggccctc gacgtaggtc
      961 agcgtgtcga acggcttgct ggctgccgag aacgcgaact gcggcgcgaa gccctgcccc
     1021 agtggctcgc cctctgcgtc ggtgatgcgg acgagcgcga agatcccgcc ctccgccttc
     1081 gcgaccccgg gcacccgctc gacgcggtcg agcagcgacg ccgagaaagg cggcggctcg
     1141 ctggtctcct gcgcgacgat ctccttcggc acgacgacga cgtcggtgcc ggacagcgcc
     1201 gtctcgaaga tgtcgtcgaa cgaccggttg atcgtgtcgg tgaggatgta ggtgccggcg
     1261 accagcgcga cgccgagcag caccgcgagg acggtgctga acgcgcgcag cttgcgtgac
     1321 gccaggctct tgagcgcgac cgaggtcatg cggctaggcg acggccttca tcagctcgag
     1381 cacgtcgtcc tcgccgccgg cgacgccgtc gtgggcgacg cggccgtcgc gcaggacgac
     1441 caggcggtcg gcgtacgccg ccgcgtgggc ctcgtgggtg accatcacca ccgtctggcc
     1501 gagctcgtcg accgagtggc gcagcagcga cagcacctcg tcggaggcct tcgagtcgag
     1561 gttgccggtt ggctcgtcgg cgaagacgac ggccggtttc gacacaagcg cccgcgcgac
     1621 cgcgacgcgc tgctgctggc cccccgacag ctcggccggg cggtgcgtac ggcggtcgcc
     1681 gaggccgacg acctcgatca ggtggtcgag ccactcggcg tcgggcttgc gcccagcgat
     1741 cgagagcggc aggcggatgt tctcctccgc ggtcagcacg ggcagcaggt tgaagaactg
     1801 gaaggcgaat ccgaccaccg cgccgtagta gcgccggtag tcgtccttga gctgcttccc
     1861 gtaccccccg cccggggtga agcgctggat cccgcccatg aagccgta
//
LOCUS       DATABV010000079        17226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_505960, whole genome shotgun sequence.
ACCESSION   DATABV010000079 DATABV010000000
VERSION     DATABV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505960"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VHF88_03215"
     CDS             complement(<1..460)
                     /locus_tag="VHF88_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2160809.1"
                     /translation="MNVSSTDGGSPTPSGGAIVTAADLQRRYGEGEAAVDALAGVSTR
                     FERGRFAAIMGPSGSGKSTLMHLLAGLDKPTGGSVVLDGVELSGLDDNRLTRLRRDKL
                     GFVFQFFNLLPVLTAEENIRLPLSIAGRKPDAEWLEHLIEVVGLGDRRTHR"
     gene            551..1240
                     /locus_tag="VHF88_03220"
     CDS             551..1240
                     /locus_tag="VHF88_03220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2160810.1"
                     /translation="MEPQAIDFPPAPAPAPAVRRPPAPLHGGPIALLRFMRRNRMLTP
                     KYARLLAGLLRRRWLTRYGRRLRSDGIAFVASDVTIQIGERGRVELGRWSWLGHGTKV
                     RCHEGTVSIGAKTVMGQECTISAYQHVSIGRECVIADRVMLIDFDHGMVEVDRPVRLQ
                     GIYKRDVRVGNNVWIGYGACILRGVTIGDNAVIGTNAVVTRDVPANAVVGGVPARLIR
                     MRDEPRTMRWE"
     gene            1231..1908
                     /locus_tag="VHF88_03225"
     CDS             1231..1908
                     /locus_tag="VHF88_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2160811.1"
                     /translation="MGVAGEGETDADRPAGVLARGPWDPERVEVRWSEQPFEPDDQTA
                     AAADAALAELGDRGSPTHDGLGARVASYEHRPDGIVLDMQPVRWALRLAGDAVDSISS
                     LCVVRDVDGRWLAGRRAPWVASWAGRWALGAGGAVEVGENPAETLARELEEEWSVTPE
                     RLSAEALIRLPTGLLLFVGLAQLPAGAEVKIDPEHDAHAWWPAAIEDWPDEADAPLRL
                     MATMLAG"
     gene            complement(1905..3092)
                     /locus_tag="VHF88_03230"
     CDS             complement(1905..3092)
                     /locus_tag="VHF88_03230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2160812.1"
                     /translation="MRVLILSWEYPPLIEGGLARHVRKLAENLVAQGVEVHVLARGLE
                     ESPPEEELDGVVVHRVREPERPRDLGEFVTWVEHMNADMLAAGVEVGDRYDFDLVHGH
                     DWLVAVAGDHLANRFRCPLAVTIHATEYGRHQGWVDKHPQSHIHGVERWMANRAERVI
                     ACSAYMREHVSDIYGLEEDRIAVIPNGIDPADLVPVDDLEALRSRFAGPDERLVLLVG
                     RLVYEKGFQLALEALPGLIERVGNVRFIVAGSGTAEQELRAQATRLGLDEHGAFVGWI
                     GDDVLHSLYRISDLTVVPSIYEPFGLVALEAMASGCPCLVADTGGLREVVPNDDVGLR
                     FHSRDPDSLGQMAERLLTDTELRDRLVAEASEHVLTFDWADVARQTAELYRDLAATRP
                     RVL"
     gene            complement(3096..4559)
                     /locus_tag="VHF88_03235"
     CDS             complement(3096..4559)
                     /locus_tag="VHF88_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEX2160813.1"
                     /translation="MSRDRGTLSLVLHSHMPYVEGFGTWPFGEEWLWEAVACVYLPLL
                     DLLEETNAPLTLGLTPVLCDQLEALAGPAGQRLIAYLRGTRRRVHDEDAAGLEQAGEP
                     ELAAEVRRAAGDYAAAAASVEACGGDLVGRFARVAAKSPVELWTSAATHAVLPLLATD
                     AGLRLQVATGIAGHQRRFSGWGGGFWLPECAYAPGLGERLAPHGVGALCVDQTAALGY
                     GSARQLEPIATAAGPIAVPVDWQTVELVWSDRSGYPADPRYRDYHRGTVHHMRPWSNG
                     GAVYSPEVARGAAREHARDFVERAVARLDAYSAQAGRPGVLCCALDTELLGHWWYEGL
                     EWLRAVIVEAEACGLELATVGDAVGRVEPVERELAASTWGTDKDLSTWDSPRVAELAF
                     AARGAELRTVVAASSGTSGGAPLERAARELLALQASDWPFQVTRELASEYPHDRLRGH
                     LAAHDAALGALADSAPVPEPSLRNLAPDLALASLAAP"
     gene            complement(4556..5320)
                     /locus_tag="VHF88_03240"
     CDS             complement(4556..5320)
                     /locus_tag="VHF88_03240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2160814.1"
                     /translation="MEPAQEYVQRDQPDGVPPLALTGERTLPDVPEENYWYRRHLAVY
                     EWVAARCAGLDVVDMACGEGYGTAVLGRSAKHVTGVDANPEAFDHARVRYGRPGVDFA
                     RDLVDSYRERCDAVVFLQTIEHVEDDAGVLRHFKDMLRPGGVAYVSTPNLLTLAPPGA
                     EKSDNPWHLREYRPQEFRALCESVFEDVELLGLFHARKLRVHELALKLGWDAVHKRLG
                     LTGRFYDWFTPAIAASDFDLRSGPLDRALDLVAVLR"
     gene            5355..6263
                     /locus_tag="VHF88_03245"
     CDS             5355..6263
                     /locus_tag="VHF88_03245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2160815.1"
                     /translation="MAAPATTEEIHEVNVRYHDLAAEHYDAKWGIDYGALGQGQVTGK
                     LAKSLGGTLGHYGRGLEIGAGTGYFGINLALAGVIDDYTASDISPGMLGVLANTAATL
                     GVDAETVCTEAAGLPFDDESFDIVFGHAILHHLPDLDGAFREFLRVLRPGGVIAFCGE
                     PSHYGDRIAELPKRAAWRLSPAWRTVLRARPARRDGSEVVEEDTLERVVDVHAFTPGT
                     LARHARGAGFGAVKVRGEELAASLFGWANRSFEASARPEDVPLLWRKYAYHGYLALQK
                     VDRAVLEPRLPPAVFYNLLVSARKPA"
     gene            complement(6264..7787)
                     /locus_tag="VHF88_03250"
     CDS             complement(6264..7787)
                     /locus_tag="VHF88_03250"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX2160816.1"
                     /translation="MKGVVAAGHPRTAQAGADVLRAGGNAVDAAVAAVLMSFAAESPL
                     TGPGAGGFMLVHTAAGESHLLDFFVAAPGRGLADPRPAPLVPIEVAFSAESIQVFHVG
                     PSSCGVYGTTLGLAEALDRFGTTALGDLTGAAAAAAREGVEVGPMQEFLLSILGPIFR
                     STPEAAAIYEPGGRPARTGERVKLPELADLLDRLGSEGPDFLYRGDVARAVSDWVLER
                     GGLITRDDLAAYEVVERRPVRARFRGRDVLTNPPPSSGGILIADALELLERLDRPGDV
                     RALAEVIASTNRARDAEFVDGLQSSGYADSFLAPRALDQVSAEIASRLGSTTHLAVID
                     GDGACATVTCSNGSCSGVVVPGTGVHLNNMLGEEDLNPSGFHNHRPGLRVPSMMSPTV
                     VLADNDVEIALGSAGSNRIRSAIVQTIANVIDGGMDAQAAVDAPRIHVEGDLIDAEPA
                     VDRDALAWLAAHEWRLRQWEDRNLYFGGVQAVARDATDQMSGGGDPRRGGAVEIVAG"
     gene            complement(7816..9252)
                     /gene="ftsZ"
                     /locus_tag="VHF88_03255"
     CDS             complement(7816..9252)
                     /gene="ftsZ"
                     /locus_tag="VHF88_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2160817.1"
                     /translation="MASGKRASMREGPLAALFRKTEEDGLEPEAKGRHQERETARREE
                     ECRPAPSAEQPRRREPAPETPVARERAEQAPVSRYDGVPTPEERLRSVFSTDIPENIL
                     DRGPSGRTQETDQEPIHQPSSPLKHDPVLRVVGVGGAGVNAVDRMIDAQVEGVEFLAV
                     NTDMQSLEQSAAPTRIHIGSDSTRGLGAGADPEAGRRAALEQYDALKSQLKGADMVFI
                     TAGAGGGTGTGAAPVVAQIAREVGALTVGIVTKPFSFEGVRRSEQADRGVEALAAEVD
                     TLITIPNTRLLSVLDKKTSMVEAFRVADDVLRQGVQGISDLITLPGLINLDFADVRTI
                     MSEAGQALLGIGMGTGETRAMDAATAAVASPLLETSVDGAQSILLSITGGNDLSLWEV
                     NEAAKAVAEAAHPDANIIFGAMVDERLEGEVWITVVATGYGGKVERRQQSPGIPADVD
                     REPRVTRRERVTSNMLGELDVPEFIPRR"
     gene            complement(9239..10048)
                     /locus_tag="VHF88_03260"
     CDS             complement(9239..10048)
                     /locus_tag="VHF88_03260"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2160818.1"
                     /translation="MANADPKHADPEAPVTPQAKVIAFTNQKGGVAKTTTTLNLAAAF
                     VESGHRALCIDLDPQGNLTMSQGIDPDSLEVSMYDVLVHDVSIREVIRKREVDVACAS
                     IDLAGAEIAMSTKIGRERSLEKAIRPIREDYDFICIDTPPSLGLLTINALTAADKVIV
                     PVQCEYLSMRGLVQLQNTLSMIRENLNPDVDIEGILPTLVDSRTVHAKEAIELLEENF
                     GDQVFASRITKTVRFAEAPVKGMSVLKYDPDGKAAYAYRQLAKEVLGNGKR"
     gene            10101..11030
                     /locus_tag="VHF88_03265"
     CDS             10101..11030
                     /locus_tag="VHF88_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX2160819.1"
                     /translation="MSALLDRRLVFVTGKGGVGKTTVAAALGLAAARAGKRTIVCEVA
                     DQERLTATFGLPPAGFREVEIEPCLSAFSVNPEDATAEWLRFQLHSRTLAGLLGGSRV
                     FQYLATAAPGLAEMVTIGKVWELAQLERKIPSAAPYDLVIVDAPATGHGLALLNAPRT
                     FAEIARVGPINRQAGIIDAFLHDSATTAVVAVALAEEMPVSETLDLERRLMAELDLGL
                     ERVFVNALLADRLTGAEVDAIQAATGSASAAARSALEASLDAHRSARVQRSQLARLRR
                     GLEAKATTLPHLLTPDVGREELERLASRLEREL"
     gene            11027..12151
                     /locus_tag="VHF88_03270"
     CDS             11027..12151
                     /locus_tag="VHF88_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX2160820.1"
                     /translation="MTARATTIDGVLAGKRVVVCAGSGGVGKTTTAAAIAAALAARGQ
                     RVIVLTIDPARRLADALGLPAVGNAECRVDQERLAAAGLGGDGELWAMTLDAKRTFDE
                     LVERNAPDAETRDAVLSNPIYRQLSNAVAGSQEYMAMEKLYELHGSGRYDVVVLDTPP
                     ARNALDFLDAPGRLSRFIDSRSLQVFRASGRAGLGLFGRGIGAVMSILERATGVDLLR
                     DLAAFFNAFGGMAEGFRDRARRVEALLCEDTTTFLLVTSPRAAAIADALHFHGKLDSS
                     RMPFGALIVNRVRAPVDARADDTELEAELNALLGEALARKVRHDLEDRNRLAERDARN
                     IELMRVDLSGGELLAVPELSGDVHDLDGLAAVAERLFERA"
     gene            complement(12152..13603)
                     /locus_tag="VHF88_03275"
     CDS             complement(12152..13603)
                     /locus_tag="VHF88_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160821.1"
                     /translation="MRNRQLHDALRAFALETAALLREDQDRGAEIEFDLDEGARRGGP
                     TLYHYRPLTAKFIADRWPRLRALPAFLPACSALGAGAAAYLRINGLRGAEAEPALQAM
                     LERLYEDATDLSFPEERFERVYGEVERTLYERSQPATVLAPVHGLVLDVERVDLGGGL
                     ALVRGGATDAPDDALWSDAGERAGEGRRDPNVLVTLTREVSPDAEPPLTEARERFRRL
                     VTGLRLWKPGGVALSTVGWRRSGEGVWQPFETEPTGVTRGAPWLLAEGEDADLREFLV
                     AIDRTRPTGSVAWALSRFEMGCGRRRESEALSDYLLGLRALLDPGAEPASTGLALRVA
                     VLCAEEGQRRGVQRRVELAQALERFVMGDSPDDDYMDAIGSDSPRMLVDEVERHLRAL
                     LRDVLCGYLDPDLRTLSDDLLLEQQPVPAAASEIESLEATGEEIGVTAVDPGEERRSA
                     DGIGIAGDASTYAGPIPVSTAADEMDADYSAPV"
     gene            complement(13649..13999)
                     /locus_tag="VHF88_03280"
     CDS             complement(13649..13999)
                     /locus_tag="VHF88_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2160822.1"
                     /translation="MASSADAPCPVCATAEIICGKWTLLVIRDLAEGRSRFCELERSL
                     AGISPRTLSLRLRALEDEGIVARHTFPEVPPRVEYSLTDKGTALLPLIEDMRRYGREW
                     LGAACGDTRATVAA"
     gene            14131..15795
                     /locus_tag="VHF88_03285"
     CDS             14131..15795
                     /locus_tag="VHF88_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160823.1"
                     /translation="MRVRLATIATLLLASAIGPATAAAAEPIMRLAEVRPGMQCSALS
                     VIRGTVPSEFEVTVIDVLRGEPETIGARILFRASGPAVDATGIGPGFSGSPIYCPDAG
                     GVRRVAGAISESVGQYGNHVALATPIEEVLGVRPAARQARRASALLRSARPIASPLTV
                     SGLSTTVRRALTAAARRAGVPLLAAPSGPALPYAPYSPTPGTSIAAGMSTGDIALAAI
                     GTVTYRDGDALWAFGHPLEGSGQRALPLLDAYVFSVIDNPVGLEEVTTYKLATAGRPI
                     GTLTSDGLGAIAGRIGTPPPMIPLTVHARDEVSGRSRTLRMRIADERALDLGSGLDEV
                     GTLAASEAMATVLGATPQRFTTSMCLRIGVRQRRKPLRFCQRYFDGFGPLDDLSAAFS
                     LVDGYKFGRLGIRNVSVRMRIRPRVREAFIVSARAPRRVRRGERIRIRLRLQHSRAGR
                     ERISFPYRVSPGAKTGRRTLTIRGSTPGGSLGGLEDLFIELLVGGGGGGGPIRSVGEL
                     AGRIAALGEREGVRATLAEKGKGPVVYSDPRLLIRGKTQVPLVVRR"
     gene            complement(15888..17000)
                     /locus_tag="VHF88_03290"
     CDS             complement(15888..17000)
                     /locus_tag="VHF88_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX2160824.1"
                     /translation="MFDLDQALRQLIAEEGSDLHLKVPVQPMIRRHGRLVPLEGSERL
                     TGEVMEGLVVQMIDDERKLAEFREDHEVDFSYSIPGLARFRVNAFRQRGSVSIVCRAI
                     PIQIKTIDELLLPPVVNELADEERGIILLTGTTGSGKSTTLAAIIDHINSNMAKHIVT
                     IEDPVEFLHPDKRSIINQREVGEDTVSFKRALRRVLRQDPDVILIGEMRDEETVRTAL
                     SAAETGHLVLSTLHTVDAPETVNRIIDFFPQAEQQQARAMLAGTLKGVISQRLVPTPD
                     GNGRIATCEILRMTGRVRDMIMDEDQTGKLPEVVAEGSYYGMQTFDQALLAHVQAGRV
                     SMEDALKAATHPHDFKLLVSSGGQRSTSVEQALAST"
BASE COUNT         2409 a         6150 c         6213 g         2454 t
ORIGIN      
        1 ggcgatgggt gcggcggtcg ccgaggccaa cgacctcgat caggtgctcg agccactcgg
       61 cgtcgggctt gcgtccggcg atcgagagcg gcaggcggat gttctcctcc gcggtgagca
      121 ccgggagcag gttgaagaac tggaagacga agccgagctt gtccctgcgc aggcgcgtga
      181 ggcggttgtc gtccaacccg ctgagctcga cgccgtcgag cacgaccgac ccgccggtcg
      241 gcttgtcgag gccggcgagc aggtgcatca acgtggactt gccggagccc gacgggccca
      301 tgatggcggc gaaccggccg cgctcgaagc gggtcgacac gccggcgagc gcgtcgactg
      361 ccgcctcgcc ctcgccgtaa cggcgctgca ggtctgcggc cgtgacgatc gccccgccgc
      421 tcggcgtcgg cgagcctcca tcggtgctcg aaacgttcat gggccgcact gttgcacgcc
      481 tcgcagcctc gggcgctccc ggcagcccgt ctcctctcgg tacggctagc cgcacctatc
      541 atccgccgac atggagccgc aggcgatcga cttcccgccg gcgccagcgc cggcgccggc
      601 ggtgcgccgt ccgcccgcgc cgctgcacgg cgggccgatc gccctgctgc gcttcatgcg
      661 acgcaatcgc atgttgacgc cgaagtacgc gcggctgctc gcgggactcc tgcggcgacg
      721 ctggctgacg cgctacggcc gccgcctgcg cagcgacggc atcgccttcg tggcgtccga
      781 cgtcacgatc cagatcggag agcgcggacg ggtcgagctc ggccgctggt cctggcttgg
      841 gcatggcacc aaggtccgtt gccacgaggg cacggtctcg atcggggcga agaccgtgat
      901 gggacaggag tgcacgatct ccgcgtatca gcacgtctcg atcggtcgcg agtgcgtgat
      961 cgccgaccgc gtgatgctga tcgacttcga ccacgggatg gtcgaggtcg accgtccggt
     1021 gcgcctgcag ggcatctaca agcgcgacgt gcgcgtcggt aacaacgtct ggatcggcta
     1081 cggggcctgc atcctgcgcg gagtgacgat cggcgacaac gcggtgatcg gcaccaacgc
     1141 cgtcgtgacc cgcgacgtgc cggccaacgc ggtcgtcggc ggcgtgccgg cacgcctgat
     1201 ccgcatgcgc gacgagccgc ggacgatgcg atgggagtag ccggcgaggg cgagacggac
     1261 gcggaccgcc cagcgggcgt gctcgcgcgc gggccctggg atcccgagcg ggtcgaggtc
     1321 cgctggagcg agcagccctt cgagcccgac gaccagaccg ccgcagccgc cgacgcggcg
     1381 ctggccgagc tgggcgaccg cgggtcgccg acgcacgacg gccttggcgc acgcgtcgcg
     1441 agctatgagc accggcccga cggcatcgtg ctggacatgc agccggtgcg ctgggcgctg
     1501 cggctcgcgg gcgacgcggt cgacagcatc tcctcgctgt gcgtggtccg cgacgtcgac
     1561 ggacgctggc tcgccggccg ccgagccccg tgggtggcga gctgggccgg tcgctgggcg
     1621 ctcggcgccg gcggcgcggt cgaggtcggc gagaacccgg ccgagacgct ggcgcgcgag
     1681 ctcgaggagg agtggtcggt gacgccggag cgactcagcg cggaggccct gatcaggctg
     1741 ccgaccggtc tgctgctctt cgtcggcttg gcgcagctgc cggcgggcgc cgaggtcaaa
     1801 atcgatcccg agcacgatgc ccatgcctgg tggccggcgg cgatcgagga ctggcccgac
     1861 gaggccgacg ccccgctgcg cctgatggcg acgatgctcg ccggctagag gacgcgcggg
     1921 cgggtcgccg ccaggtcgcg gtagagctcg gccgtctgcc gtgccacgtc ggcccagtcg
     1981 aacgtcagca cgtgctcgga cgcctcggcg acgaggcgat cgcgcagctc ggtatcggtc
     2041 agcagccgtt ccgccatctg accgagcgag tcggggtcgc gcgagtggaa gcgcagcccg
     2101 acgtcgtcgt tggggacgac ctcgcgcagg ccgccggtgt cggcgaccag gcaggggcag
     2161 ccagacgcca tcgcctcgag cgccacgagg ccgaagggct cgtagatcga cggcaccacg
     2221 gtcaggtcac tgatcctgta gagcgagtgc aggacgtcgt cgccgatcca gccgacgaag
     2281 gccccgtgct cgtcgagccc gagacgcgtc gcctgcgcac gcagctcctg ctccgcggtt
     2341 ccggagccgg cgacgatgaa gcggacgttg ccgacgcgct cgatcaatcc cggcagcgcc
     2401 tccagcgcga gctggaagcc cttctcgtag acgagccggc cgaccagcag gaccagccgc
     2461 tcgtcgggcc cggcaaagcg cgagcgcagc gcctcgaggt cgtccaccgg gaccaggtcg
     2521 gccggatcga tcccgttcgg gatcacggcg atccggtcct cctcgagccc gtagatgtct
     2581 gagacgtgct cgcgcatgta ggccgagcag gcgatcacgc gctcggcgcg gttcgccatc
     2641 cagcgctcga cgccgtggat gtggctctgc gggtgcttgt cgacccagcc ctgatgacgg
     2701 ccgtactcgg tcgcgtggat agtgacggcg agcggacagc ggaagcggtt ggcgaggtgg
     2761 tcgccggcga cagcgacgag ccagtcgtga ccgtggacga ggtcgaagtc gtagcggtcc
     2821 cccacctcca caccggcggc cagcatgtcg gcgttcatgt gctcgaccca agtgacgaac
     2881 tcgccgaggt cgcgcggacg ctccggctcg cgcacgcggt ggacgaccac gccgtcgagt
     2941 tcctcctcag gcgggctctc ctcgagcccg cgcgccagca cgtgcacctc gaccccctgg
     3001 gcgacgaggt tctcggcgag cttgcgcacg tggcgagcga ggccgccctc gatcagcggc
     3061 gggtactccc aggagaggat caggacgcgc atggctcagg gtgcggctag agacgccagc
     3121 gccaggtcgg gcgccaggtt gcggagggaa ggctccggca cgggcgcgga gtctgccaga
     3181 gcgccgagcg cggcgtcgtg cgcggcgagg tgcccgcgca gtcggtcgtg ggggtactcc
     3241 gaggcgagct cgcgggtgac ctggaacggc cagtcgctgg cctggagcgc aagcagctcg
     3301 cgggcagcgc gctcgagcgg cgctccccca gaggtgcccg agcttgccgc gacgacggtt
     3361 cgcagctccg ccccgcgggc cgcgaacgcc agctcggcga cacgcgggga gtcccaggtc
     3421 gagaggtcct tgtcggtccc ccatgtcgaa gccgccagct ctcgctcgac cggctcgacc
     3481 cgcccgaccg catcgccgac ggtcgccagc tcgaggccgc atgcctcagc ctcgacgatg
     3541 accgcgcgca gccactccag accctcatac caccagtggc cgagcagctc ggtgtcgagc
     3601 gcgcagcaga ggactccggg ccgccccgcc tgcgcggagt aggcgtccag acgcgcgact
     3661 gcgcgctcga cgaagtcacg cgcgtgctcg cgcgccgcgc cgcgggcgac ctccggcgag
     3721 tagaccgcgc cgccgttgct ccacgggcgc atgtggtgga cggtcccgcg gtggtagtcg
     3781 cggtaacgcg ggtcggcggg atagccgctg cgatcactcc agaccagctc gaccgtctgc
     3841 cagtcgaccg gcacggcgat cggcccggcc gcggtggcga tcggctcgag ctgccgcgcc
     3901 gagccgtatc cgagggcggc ggtctggtcg acgcacaagg cccccacccc atgcggcgcc
     3961 aatctctccc ccaggcccgg ggcgtaggcg cactcgggca gccagaaccc gccaccccag
     4021 cccgagaagc ggcgttggtg cccggcgatg ccggtcgcga cctgcagccg caggcccgcg
     4081 tcggtcgcga gcagtggcag caccgcgtgc gtcgcggctg acgtccacag ctcgaccgga
     4141 ctcttcgccg ccacccgcgc gaaccggccg acgaggtccc cgccgcaggc ctccacagac
     4201 gccgcggccg ccgcgtagtc gcccgcggcc cgcctgacct cggccgccaa ctcgggctcg
     4261 ccggcctgct cgaggccggc ggcgtcctcg tcgtgaaccc gccggcgcgt gccgcgcaga
     4321 taggcgatca gtcgctggcc cgcgggtccg gcaagcgcct cgagctgatc gcagagcacc
     4381 ggcgtcagac cgagcgtcag cggtgcgttg gtctcttcga gcaggtcgag cagcggcagg
     4441 tagacgcagg cgaccgcctc ccagagccac tcctcgccga acggccaggt cccgaagccc
     4501 tcgacgtagg gcatgtgcga gtggaggacg aggctgagcg tgccgcggtc gcggctcatc
     4561 gcaggacggc gacgaggtcc agcgcacggt cgagcggacc ggagcgcagg tcgaagtcgc
     4621 ttgccgcgat cgccggagtg aaccagtcgt agaagcgacc ggtcaggccg aggcgcttgt
     4681 ggaccgcatc ccagccgagc ttcagcgcca gctcgtgcac gcgcagcttg cgtgcgtgga
     4741 agaggccgag cagctcgacg tcttcgaaga cggactcgca gagcgccctg aactcctgcg
     4801 gccggtactc gcgcaggtgc cacgggttgt cggacttctc agcccctggg ggggcgagcg
     4861 tcagcaggtt cggcgtcgat acgtacgcga cgccgccggg ccgcagcatg tccttgaagt
     4921 gacgcagcac gccggcgtcg tcctcgacgt gctcgatcgt ctggaggaag accacggcgt
     4981 cgcagcgctc gcggtaggag tcgacgaggt cgcgcgcgaa gtcgaccccg ggccggccgt
     5041 aacgcacccg ggcatggtcg aacgcctcgg ggttggcgtc gaccccggtg acgtgcttgg
     5101 cgctgcgccc cagcaccgcg gtgccgtagc cctcgccaca cgccatgtcg acgacatcga
     5161 gtccggcgca gcgcgcggcg acccactcgt agacggcgag atgacggcgg taccagtagt
     5221 tctcctccgg cacgtcgggc agcgtgcgct cgccggtgag agcaagcggg ggcacaccgt
     5281 cgggctggtc gcgctgcacg tactcctggg ccggctccac agccgcgtga gtctacggat
     5341 acccttcccc gcgaatggcg gcgcctgcca caacggagga gatccacgag gtcaacgtcc
     5401 gctaccacga cctcgcggcc gagcactacg acgccaagtg gggcatcgac tacggcgcgc
     5461 tcggtcaggg ccaggtcacc ggcaagctcg cgaagtcgct cggcgggacg ctcggccact
     5521 acgggcgcgg cctcgagatc ggcgccggca ccggctactt tgggatcaac ctcgccctgg
     5581 ccggggtgat cgacgactac accgccagcg acatctcgcc cggcatgctg ggtgtgctcg
     5641 cgaacaccgc cgcaacgctt ggggtggacg ccgagacggt ctgcacggag gccgccggcc
     5701 tgccgttcga cgacgagagc ttcgacatcg tcttcgggca cgccatcctc caccacctgc
     5761 cggacctcga cggcgccttc cgcgagttcc tgcgcgtgct ccgtcccggt ggcgtgatcg
     5821 ccttctgcgg cgagccatct cactacggcg accgtatcgc cgagctgccc aagcgcgccg
     5881 cctggcggct gtcgccggcg tggcggaccg tcctgcgcgc gcgacccgcg cgacgcgacg
     5941 gctcggaggt agtcgaggag gacaccctcg agcgggtcgt cgacgtccac gccttcactc
     6001 ccggcacgct ggctcgccac gcgcgcggcg ctggcttcgg cgcagtcaag gtgcgcggcg
     6061 aggagctggc ggcgagcctc ttcggctggg ccaaccgctc gttcgaggcg agcgcgcggc
     6121 cggaagacgt gccgctgctc tggcgcaagt acgcctacca cggctacctg gcgctgcaga
     6181 aggtggatcg cgcggtcctg gagccacgcc tgccgccggc ggtcttctac aacctgctgg
     6241 tctcggcgcg caagccggcc tgatcagcca gcgacgatct ccacggcgcc accgcggcgc
     6301 ggatcgccgc cgccggacat ctggtcggta gcgtcgcgcg caacggcttg gacgccgccg
     6361 aaatagaggt tgcggtcctc ccactggcgc aggcgccact cgtgcgcggc cagccacgcg
     6421 agggcgtcgc gatcgaccgc gggctcggca tcgatcaggt ctccctcgac gtggatgcgc
     6481 ggcgcgtcta cggccgcctg cgcgtccatg ccgccgtcga tgacgttggc gatcgtctgg
     6541 acgatcgccg agcggatgcg gttcgagccc gcggagccga gagcgatctc gacgtcgttg
     6601 tcggccagca cgaccgtcgg ggacatcatc gaggggacgc gcaggccggg ccggtggttg
     6661 tggaagcccg acgggttgag gtcctcctcg ccgagcatgt tgttgaggtg gacgccggtg
     6721 ccggggacga cgacgccgga gcaggagccg ttggagcagg tgacggtcgc gcaggcgccg
     6781 tcgccgtcga tgacggcgag gtgggtggtc gaaccgagtc gcgaggcgat ttcggctgag
     6841 acctggtcga gcgctcgcgg ggcgaggaag ctgtcggcgt agccgctgct ttggagcccg
     6901 tcgacgaact cggcgtcgcg ggcacggttg gtcgaggcga tcacctcggc gagcgcgcgg
     6961 acgtcgccgg gacggtcgag tcgctcgagc agctcgagcg cgtcggcgat cagaatgcct
     7021 ccggacgagg gtggcgggtt ggtcaggacg tcgcgtccgc ggaagcgcgc gcggaccggg
     7081 cggcgctcga cgacctcgta ggcggcgagg tcgtcgcggg tgatcaggcc accgcgctcc
     7141 agcacccagt cgctgacggc ccgggccacg tcgccgcgat agagaaagtc cggcccctcg
     7201 ctcccgagcc ggtcgagcag gtcggcgagc tccggtagct tgacgcgctc gcccgtgcgg
     7261 gcgggccggc cgcccggctc gtagatcgcg gccgcctcgg gcgtggagcg gaagatcggg
     7321 ccgaggatcg agagcaggaa ctcctgcatc ggccccacct ccacgccctc gcgcgccgca
     7381 gcggcggcag caccggtgag gtcaccgagc gcggtcgtcc cgaaccgatc aagcgcctcg
     7441 gcgagaccca gcgtcgtgcc gtacaccccg cacgacgacg ggccaacgtg gaagacctgg
     7501 atcgactcgg ccgagaacgc gacctcgatc gggacgagtg gggcaggcct cggatctgcg
     7561 aggccgcggc cgggagcggc gacgaagaag tcgagcaggt ggctctcgcc ggccgccgtg
     7621 tggaccagca tgaacccgcc cgcgcccggc cccgtcagcg gcgactccgc cgcaaacgac
     7681 atcagcaccg ccgcgaccgc cgcgtcgacg gcattacctc ccgcgcgcag gacgtccgcc
     7741 ccggcctgtg ccgttcgcgg atggccggct gcgacgacgc ccttcacgag ggcgcgagac
     7801 tagtgcctct gggccttagc ggcgcggaat gaactcaggg acgtcgagct cgccgagcat
     7861 gttgctcgtg actcgctcgc ggcgcgtgac gcgcggctcg cggtcgacgt ccgctgggat
     7921 cccggggctc tgctgacggc gctcgacctt gccgccgtag ccggtggcga cgaccgtgat
     7981 ccagacctcg ccctcgagcc gctcgtcgac catcgcgcca aagatgatgt tggcgtcggg
     8041 gtgagcggcc tcggcgaccg ccttggcggc ctcgttgacc tcccagagcg aaaggtcgtt
     8101 gccgccggtg atcgacagca ggatcgactg ggcaccgtcg acggaggtct cgagcagcgg
     8161 cgaggcgacc gccgccgtgg cggcgtccat cgcacgcgtc tcccccgtgc ccatgccgat
     8221 tccgagcagc gcctggccgg cctcgctcat gatcgtccgc acgtcggcga agtcgaggtt
     8281 gatcaggccc gggagtgtga tcaggtcgga gatgccctgg acgccttggc gcagcacgtc
     8341 gtcggcgacg cggaaggcct cgaccatcga ggtcttcttg tcgagcaccg agagcaggcg
     8401 tgtgttcggg atcgtgatca gcgtgtcgac ctcggcggcg agcgcctcga cgccacgatc
     8461 ggcctgctcg ctgcggcgca cgccctcgaa ggagaacggc ttggtgacga tgccgacggt
     8521 caacgcgccg acctcacggg cgatctgcgc gacgacaggg gccgcccccg tgccggtgcc
     8581 gccaccggcg ccggcggtga tgaagaccat gtccgcgccc ttgagctggc tcttgagcgc
     8641 gtcgtactgc tcgagcgccg ccctgcggcc ggcctcgggg tccgcgccgg cgccgagccc
     8701 gcgcgtcgag tcgctgccga tgtggatgcg ggtcggcgcg gccgactgct cgagcgactg
     8761 catgtcggtg ttgaccgcga ggaactcgac gccctcgacc tgcgcgtcga tcatccggtc
     8821 gaccgcgttg acgccggctc cgccgactcc gacgacgcgc agcacgggat cgtgcttgag
     8881 cggcgaggac ggctggtgga tcggctcctg gtcggtctcc tgggtccggc cagacggccc
     8941 gcgatcgagg atgttctccg ggatgtcggt cgagaagacc gagcgcaggc gctcctcggg
     9001 cgtcggcacg ccgtcgtagc ggctgaccgg cgcctgctcg gcgcgctcgc gagctaccgg
     9061 cgtctcgggc gcgggctcgc gccgtcgcgg ctgctccgcc gacggagcgg ggcgacactc
     9121 ctcctcgcga cgagcggtct cgcgctcctg gtggcggccc ttggcctcgg gctcgaggcc
     9181 gtcctcctcg gtcttgcgga acagggccgc gagcggcccc tcccgcatgc tcgcgcgctt
     9241 accgcttgcc attgccgagg acctccttag cgagctgacg gtaggcgtag gcagccttgc
     9301 cgtcagggtc gtacttgagc acggacattc ccttgacggg cgcctcagcg aagcggaccg
     9361 tcttggtgat gcgcgaggcg aagacctggt cgccgaagtt ctcctccagc agctcgatcg
     9421 cctcctttgc gtggaccgtc cggctgtcga ccagggtcgg caggatgccc tcgatgtcta
     9481 cgtcgggatt gaggttctcg cgaatcatcg agagcgtgtt ctgcagctgc accagtcccc
     9541 gcatggacag atactcgcat tggacgggga cgatcacctt gtcagccgcc gtgagggcgt
     9601 tgatcgtgag cagcccgagg ctcggaggag tgtcgatgca gatgaagtcg tagtcctcgc
     9661 ggatcggccg gatcgccttc tccagcgagc gctcacggcc gatcttcgtc gacatcgcga
     9721 tctccgcccc ggcgaggtcg atcgacgcgc aggcgacgtc gacctcgcgc ttgcggatca
     9781 cctcgcggat cgagacgtcg tggacgagca cgtcgtacat cgagacctcg agcgagtcgg
     9841 ggtcgatgcc ctggctcatc gtcaggttgc cctgcgggtc gaggtcgatg cagagcgcgc
     9901 ggtgcccact ctcgacgaac gccgcggcca ggttgagcgt cgtggtcgtc ttggccacgc
     9961 cgcccttctg gttggtgaaa gcgatgacct tcgcctgtgg cgtcactggt gcctccggat
    10021 cggcgtgctt ggggtccgcg tttgccattg cccctgccta ttcgcaggga cgggggcgat
    10081 tcctcctacc cttcccggcc gtgagcgccc tcctagacag gcggctggtc ttcgtcacgg
    10141 gcaagggcgg agtcggcaag acgacggtcg ccgcggcgct cgggctggcg gcggcgcggg
    10201 ctggcaagcg gacgatcgtc tgcgaggtgg cggaccagga gcgcctgacc gcgaccttcg
    10261 ggctgccgcc ggccggcttc cgcgaggtcg agatcgagcc ctgcctgagc gccttctcgg
    10321 tcaacccgga ggacgcgacc gccgagtggc tgcgcttcca gctccactcg cgcacgctgg
    10381 cggggctgtt gggcggaagc cgggtcttcc agtacctcgc gaccgccgcg ccggggctgg
    10441 ccgagatggt cacgatcggc aaggtctggg agcttgccca gctcgagcgc aagattccga
    10501 gcgccgcccc gtacgacctg gtcatcgtcg acgcgccggc caccggtcac ggcttggcgc
    10561 tgctgaacgc gccgcgaacg ttcgccgaga tcgcgcgcgt cggcccgatc aaccgccagg
    10621 ccggcatcat cgacgccttc ctgcacgact cggcgacgac cgccgtagtc gccgtggcgc
    10681 tcgccgagga gatgccggtc agcgagacgc tcgacctcga gcggcgactg atggcggagc
    10741 tcgacctcgg gctggagcgg gtgttcgtca acgcgctgct ggcggaccgc ctcaccggcg
    10801 ccgaggtcga cgccatccaa gccgcgaccg gcagcgcctc cgcggcggcg cgctccgcgc
    10861 tcgaggcgtc gctcgacgca caccgcagcg cgcgcgtcca gcgctcgcag ctggctcggc
    10921 tgcggcgcgg cctcgaggcc aaggcgacga cgttgccgca tctgctgacc ccggatgtcg
    10981 gacgcgagga gctggagcgc ctcgccagcc gactggagcg cgagctgtga cagcgcgcgc
    11041 gacgacgatc gacggggtgc tcgccggcaa gcgggtcgtt gtctgcgccg gctcgggagg
    11101 cgtaggcaag acgacgaccg ccgctgcgat agccgcggcg ctggcggcgc gcggtcagcg
    11161 cgtgatcgtg ctgacgatcg atcccgcccg gcgcctcgcc gacgcgctcg ggctacctgc
    11221 ggtcggcaac gcggaatgcc gcgtcgacca agagcggctc gccgctgccg ggctcggcgg
    11281 cgacggcgag ctgtgggcga tgacactcga cgccaagcgg accttcgacg agctggtcga
    11341 gcgcaacgcg cccgacgcag agacccgcga cgccgtgctc tcgaacccga tctaccgcca
    11401 gctctccaac gccgtcgccg gctcgcagga gtacatggcg atggagaagc tctacgagct
    11461 gcacggctcg ggccgctacg acgtcgtcgt gctcgacacg cccccggccc gcaacgcact
    11521 cgacttcctc gatgcgcccg ggcggctctc gcgcttcatc gactcgcgct cgttgcaggt
    11581 ctttcgtgcc tccggacgcg ccggtctggg cctcttcggg cgcgggatcg gcgcggtgat
    11641 gtcgatcctc gagcgagcga cgggcgtcga cctgctgcgc gacctggccg ccttcttcaa
    11701 cgccttcggc ggcatggccg agggcttccg cgatcgcgct cgccgcgtcg aggcgctgct
    11761 ctgcgaggac acgacgacgt tcctgctggt cacatcgccg cgcgccgcgg cgatcgccga
    11821 cgcgctccac ttccacggca agctcgacag ctcgcggatg cccttcggag cgttgatcgt
    11881 caaccgcgtc cgcgcgccgg tcgacgcgcg cgccgacgac accgagctcg aggccgagct
    11941 gaacgcgctg ctcggcgagg cgctcgcacg caaggtccgt cacgacctcg aggaccgcaa
    12001 ccggctcgcc gagcgcgacg cgcgcaacat cgagctgatg cgcgtcgacc tcagcggcgg
    12061 cgagctgctc gctgttcccg aactgtcggg cgacgtccac gacctcgacg gcctcgccgc
    12121 cgtcgccgag cggctgttcg agcgcgccta gctaaacggg tgccgagtaa tcggcgtcca
    12181 tctcgtctgc tgccgtgctc accggaatcg gcccagcgta ggtcgaggcg tcgcctgcga
    12241 taccgattcc gtctgcgctc ctccgctctt cgccggggtc gacggccgtg acgccgatct
    12301 cctccccggt cgcctcgagc gactcgatct cggatgccgc ggcgggtacc ggctgctgct
    12361 cgagcaggag gtcgtcggac agcgtgcgca ggtcgggatc gagatagccg cagagcacgt
    12421 cgcgcagcag cgcgcgcagg tggcgctcga cctcgtcgac gagcatccgc ggcgagtcgg
    12481 acccgatcgc atccatgtag tcgtcgtcgg gggagtcgcc catgacgaac cgctcgagcg
    12541 cctgcgccag ctcgacacgc cgctggacgc cgcgccgctg gccttcctcg gcacagagca
    12601 cggccacgcg caaggcgagc ccggtgctcg ccggctccgc gccgggatcg agcagcgccc
    12661 gcaggccgag caggtagtcg gagagcgcct cggactcgcg ccggcgaccg cagcccatct
    12721 cgaagcgcga cagcgcccac gcgaccgatc cggtcgggcg ggtgcggtcg atcgccacga
    12781 ggaactcgcg caggtccgcg tcctcgccct cggcgagcag ccacggggcg ccgcgcgtga
    12841 cgccggtcgg ctcggtctcg aacggttgcc agacgccctc gccgctgcgc cgccagccga
    12901 cggtcgacaa cgccacgccg ccgggcttcc acagccgcag gccggtcacc agccggcgga
    12961 agcgctcgcg cgcctcggtc agcggcggct cggcgtcggg gctcacctca cgggtcaaag
    13021 tgaccaggac gttgggatct cgccgcccct cgcccgcacg ctcgccggcg tcgctccaga
    13081 gcgcgtcgtc gggcgcgtcg gtcgccccgc cgcggacgag cgcgaggccg ccgccgaggt
    13141 ccacgcgctc gacgtcgagc acgaggccgt ggacgggcgc cagcaccgtc gccggctggc
    13201 tgcgctcgta caacgtccgc tccacctcgc catagacgcg ctcgaagcgc tcctcgggga
    13261 acgagaggtc ggtcgcgtcc tcgtagaggc gctcgagcat cgcctgcagc gccggctcgg
    13321 cctccgcgcc gcgcagcccg ttgatccgca ggtaggccgc ggcgccggcc ccgagcgcgg
    13381 agcaggcggg gaggaacgcc ggcagcgcgc gcagccgcgg ccagcggtcg gcgatgaact
    13441 tcgcggtcag cgggcggtag tggtagagcg tcgggccgcc acggcgcgcg ccctcgtcga
    13501 ggtcgaactc gatctcggcg ccgcgatcct gatcctcgcg cagcagcgcc gcggtctcga
    13561 gcgcgaaggc gcgcagcgcg tcgtgaagct gtcgattccg catccccgcc acttccgcgc
    13621 cggcgcgcgg aaccctgcaa ccgaagcgct aggcggcgac cgtggcgcgt gtatccccgc
    13681 aggcggcgcc gagccactcg cggccatagc ggcgcatgtc ctcgatcagc ggcagcagcg
    13741 ccgtgccctt gtccgtcagc gagtactcga cgcgcggcgg cacctcgggg aaggtgtggc
    13801 gcgcgacgat cccctcgtcc tcgagtgcgc gcaggcgcag cgacagcgtc cgcggtgaga
    13861 tcccggcgag cgagcgctcc agctcgcaga agcgcgaccg gccctcggcg aggtcgcgga
    13921 tcacgagcaa cgtccacttg ccacagatga tctcggcggt ggcgcagacg ggacagggtg
    13981 catcggcgga ggaagccacg acaaccccag gttagcgcaa tgcttcacga actgaagtgt
    14041 cgggacgggc gccctcgcag gtgtctcgca gaccgtcacg aatcccgcta agtgcgaccg
    14101 tttgccgcca cctcggatag cctcgcggcg atgagggttc ggctcgcgac gatcgcgaca
    14161 ctgctgcttg cgtccgcgat cggtcccgct accgccgcag ccgctgagcc gatcatgaga
    14221 ttggcggagg tccggccggg catgcagtgc agcgcgctgt cggtgatccg cggcacggtc
    14281 ccgtcggagt tcgaggtcac ggtgatcgac gtgctgcgtg gcgagcccga gacgatcggc
    14341 gcgcgcatcc tcttccgggc ctccggaccg gcggtcgacg cgaccggcat cggacccggt
    14401 ttctccggct cgccgatcta ctgccccgat gccggtggcg tgcggcgggt cgccggcgcg
    14461 atctccgaga gcgtcggtca gtacggcaac cacgtggcgc tcgcgacacc gatcgaggag
    14521 gtgctcggag tgcggcccgc cgcgcggcag gcccgacggg cgagcgcgct gctgcgctcg
    14581 gcacggccga tcgccagccc gctgaccgtc tccggcctct cgacgacggt gcgtcgcgcg
    14641 ctgaccgccg ccgcacgacg cgcgggcgtc ccgctgctcg ccgctccctc cgggcccgcc
    14701 ctgccctacg ccccctactc acctaccccc gggacttcga tcgccgcggg gatgtcgacc
    14761 ggcgacatcg cgctggccgc gatcggaacg gtcacgtatc gcgacggcga cgcgctgtgg
    14821 gccttcggcc acccgctcga gggcagtggc caacgggcgc tgccgctgct cgacgcatac
    14881 gtcttctcgg tgatcgacaa cccggtcggg ctcgaggagg tcaccaccta caagctcgcg
    14941 accgctgggc gcccgatcgg cacgctgacc agcgacggcc tcggcgcgat cgccggccgc
    15001 atcggcactc cgccgccgat gatcccgctg accgtccacg cgcgcgacga ggtcagcgga
    15061 cgctcacgga cgctgcgcat gcggatcgcc gacgagcgcg cactcgatct gggaagcggc
    15121 ctcgacgagg tcgggacgct ggccgccagc gaggcgatgg cgaccgtcct gggcgccacg
    15181 ccgcagcgct tcacgacctc gatgtgcttg cgcatcggcg tccgtcaacg gcgcaagccg
    15241 ttgcgcttct gccagcgcta cttcgacggc ttcggcccgc tcgacgatct gtcggcggcg
    15301 ttctcgctcg tcgacggcta caagttcggc cgccttggga tccgcaacgt gtcggtccgg
    15361 atgcggatcc gcccgcgcgt gcgcgaggcc ttcatcgtga gcgcccgggc tccgcgccgc
    15421 gtccgccgcg gcgagcggat tcgcatccgg ctgcgtctcc agcacagccg cgccggccgt
    15481 gagcggatct ccttccccta ccgcgtgtcg cccggcgcga agacgggtcg gcgcaccttg
    15541 acgatccgcg gcagcacccc gggcggcagc ctcggcggcc tcgaggacct cttcatcgag
    15601 ctgctcgtgg gaggcggcgg aggcggcggc ccgatccgct cggtgggcga gctcgcgggc
    15661 cggatcgccg cgctcggcga gcgcgagggc gtccgtgcga cgctcgccga gaagggcaag
    15721 ggcccggtcg tctacagcga cccgcggctg ctgatccgcg gcaagacgca ggtccccctg
    15781 gtcgtccgaa ggtgacaccg gcctcctcct gggtgtggct ggcaaggaag cagggagccg
    15841 tggcggggcc cgccccgcga ggttcccgat gacgccgcca ggcgcggtca ggtggaggcc
    15901 agtgcctgct cgacggaggt cgagcgctga ccgccggagg agacgagcag cttgaagtcg
    15961 tgcgggtggg tggccgcctt gagcgcgtcc tccatcgaga cgcggccggc ctggacgtgt
    16021 gcgagcaggg cctggtcgaa ggtctgcatg ccgtagtagg agccctcggc gacgacctct
    16081 ggcagcttgc cagtctggtc ctcgtccatg atcatgtcgc gcacccgtcc ggtcatgcgc
    16141 aggatctcgc aggtggcgat ccgcccgttg ccgtcgggcg tcggcaccaa ccgttgggag
    16201 atcacgccct tgagcgtgcc ggcgagcatc gcgcgcgcct gctgctgctc ggcctgcggg
    16261 aagaagtcga tgatccggtt gacggtctcg ggcgcgtcga cggtgtgcag cgtcgacagc
    16321 accaggtggc cggtctcggc cgctgacagc gccgtgcgaa cagtctcctc gtcgcgcatc
    16381 tcgccgatca ggatcacgtc ggggtcctgg cgcaggacac ggcgcagcgc gcgcttgaag
    16441 gaaacggtgt cctcgcctac ctcgcgctgg ttgatgatcg accgcttgtc cggatggagg
    16501 aactcgaccg ggtcctcgat cgtgacgatg tgcttcgcca tgttcgagtt gatgtgatcg
    16561 atgatcgccg cgagcgtcgt cgacttgccg gagcccgtcg tgccggtcag caggatgatc
    16621 ccgcgctcct cgtcggcgag ctcgttgacc accggcggca gcagcagctc gtcgatcgtc
    16681 ttgatttgga tcgggatcgc gcggcaaacg atcgataccg acccacgctg gcggaatgca
    16741 ttgacgcgga agcgcgccag gcccgggatc gagtacgaga agtcgacctc gtggtcctcg
    16801 cggaactcgg cgagcttgcg ctcgtcgtcg atcatctgca cgacgaggcc ctccatgacc
    16861 tcaccggtca gccgctcact tccctcgagc ggcaccagcc gcccgtggcg gcggatcatc
    16921 ggctgcacgg ggaccttcag gtggaggtcc gagccctcct cggctatcag ctggcgcagg
    16981 gcctgatcga gatcgaacac gccctatcca tcggcccggg ggccgcgcgg cttgagcccc
    17041 gcctggcggc gctacgcgac gtcgcggagc ttctgagcct ccgcgagcga ctgcagcttc
    17101 ttgagcgact ggttctcgat ctggcggatc cgctcgcgcg tcacgttgaa cgtgcggccg
    17161 acctcgtcga gcgtgcgcgg gtgctcaccg ccgagcccat agcggagctc gagcacgcgc
    17221 cgctcg
//
LOCUS       DATABV010000080        10087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2273987, whole genome shotgun sequence.
ACCESSION   DATABV010000080 DATABV010000000
VERSION     DATABV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273987"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /gene="ccsA"
                     /locus_tag="VHF88_03295"
     CDS             complement(<1..1217)
                     /gene="ccsA"
                     /locus_tag="VHF88_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX2160825.1"
                     /translation="MAGLGSACLLVGLLTAVYAIGASLYGARADSRAAVISGRRAIYC
                     VAALMVTATVVLQSAFLRSDFSFKLVAEGSSTDTPTFYKFTAMWATQDGSLLLWALLM
                     SLFASAVLFATRRGLRDIAPYATAVLGGIAAFFLLLMVGWENPFTTLAVAPVEGAGLN
                     PLLRHPAMMIHPPMLYTGYVGFSIPFAFAIGALIVRQTGADWIRATRRFALIAWTFLG
                     AGIMLGALWSYSELGWGGYWAWDPVENASLMPWLIGTAFIHSIMVQEKRGMLKVWNVS
                     LIVSTFVLALLGTFLVRSGILDSIHAFGASTIGKQFLTFIAIVILGSTALIVSRLDDL
                     RSDAKLDSLMSREAFFLVNNLVLVGLCVVIFWGTFFPLISEALSGEEASVGPPWFNRV
                     TTPLALVLVALAA"
     gene            complement(1219..1623)
                     /locus_tag="VHF88_03300"
     CDS             complement(1219..1623)
                     /locus_tag="VHF88_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX2160826.1"
                     /translation="MDPSRKRRLRLFVALSAAVLLAGALVYTSFSASTQAKQPSDLLT
                     GAEPGRSYELTGKVVKDSVDKDGDRLTFRIRDRDGRESVPVTYAGVVPDPFREGREVI
                     VSGQLEGGTFVAERDSLITKCPSKFTEDEQST"
     gene            1795..3564
                     /locus_tag="VHF88_03305"
     CDS             1795..3564
                     /locus_tag="VHF88_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX2160827.1"
                     /translation="MTPASAAPPRPIELRSGWEVLEGDWRPTAVPSTFEGLARAPFYG
                     GTLKLYRLRFTAPDVEGYDWAFDFEQVRRRATVSLNGRRIGLSIDPYTPFRVPARGLL
                     PGEVNTLEVLVDSRKDPRLPEGWWNWGGIVRPVKMVPIGPAHVRDLALLPSVRCSGPA
                     TDCSASLLIDGILEKLPRQPPPSEVVDGRRVVLPPPRPLLKIRLEAPDGEVTFHRAWL
                     RGSSAGRRRQKIQIAMPPPQLWSPELPQLYDARVTLKYDGEVTQVEKRKLGFRSIEVR
                     DGMLLLNNRPINLRGASIHEDAPGRGAAINGYDMDTTVRELKELGANVTRAHYVLSQG
                     LLNRFDKAGILVYNQAPVWQRDHGANLLRRPIDRARAVAQVERTVLAARGHPSVIVHS
                     VANELTFTPDRRPASRRFLLDAAKAARDLDPLVPIAVDIKTRPWLPRQRTYDAFDLIG
                     INQYFGWYPWVEDFAGLEPFLNRMRAYYPEQALVMTEFGAEGRPDMAGDPIDVKGSYE
                     FQANHVARTIDLVDRLPFMSGAIHWTLREFEIFPDWRGGASGHEGPNDNIRHHKGVLT
                     YTGERKPAWQVLYDRFVRTPFYR"
     gene            complement(3573..4568)
                     /locus_tag="VHF88_03310"
     CDS             complement(3573..4568)
                     /locus_tag="VHF88_03310"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX2160828.1"
                     /translation="MQTAFDAPHDPVELGGIWTAGDVETAIASSTGVELSDRVTDAFM
                     KVIAAAPDGRTGYAAQSAIAAAALDPAALAERAAVKAAVDGAPVSLTPGDYTVVMDAH
                     AVLDLLWVLAETAFDGLAHAEGRGALTERLGTRVVSPAINLSDSPRFQATLPRAFDAE
                     GVPKAPLPLIQDGVAHRVVHDTRSAAVAGGTTASTGHALVAGGQAAGPRPTNLVLIGG
                     GAADETDLCAPVERGIYVTRLWYTNVLRPTESRFTAVTRDGTFLIEDGQITRPLEELR
                     ITDSALSILSHVEALGARSVLTSDGELYGRRFATGSVCPPLRAAGVHFSGSALQP"
     gene            complement(4463..4942)
                     /locus_tag="VHF88_03315"
     CDS             complement(4463..4942)
                     /locus_tag="VHF88_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX2160829.1"
                     /translation="MSEALDTARRALAETSGEALVTVTRERSAMLRFARSRPTQATSI
                     DDLTVEIAILRHGHVGRAATNRLHREALGECARAAALAAEAAADSAGEGHYPGFPGAA
                     TPRAHDDSTPRPRGSTRGPAQRRCRRPSTRRTTRSSWAASGPRATSRRRSPRRPASS"
     gene            complement(4939..6375)
                     /locus_tag="VHF88_03320"
     CDS             complement(4939..6375)
                     /locus_tag="VHF88_03320"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX2160830.1"
                     /translation="MRDLLGDLMDAAAGRVDYADMRHVRLREESVAMRNGELDELDAR
                     DEEGFGARVQLGGGWGFAAAAGSDRTAAEDALTRAIAIAEAQPRVGSQPLTPEPAATG
                     RHVSPFATDPFAVSLDEKLALLHETDCALREQPRVTVAFTALQSRREQKVLASTEGTL
                     CEQELTDCGGVVSATAVHGDDAQTRSYPASHAGSVAQAGWEHVLALDLPGHAPRVAEE
                     AVALLDAPPCPEGPTTLVLAGEQLGLQIHESVGHAVELDRVLGYEASYAGTSWVPADG
                     GGALRYGSDAMSVTADATVPGAIGSYRWDDEGVPGQSISIVRDGVLRGFLSNRGSAAE
                     IGLERSGGCARAEGFARQPIVRMSNVSLDPGQGGSLADLLSGVESGIYIDTNRSWSID
                     SRRLHFQFGGEAAREIVDGRLGRLLRDPVYAGVTPKFWGSLDAVCSAEEWALTSFTDC
                     GKGEPGQLARVAHGCAPARFRGVQVGAG"
     gene            6463..6678
                     /locus_tag="VHF88_03325"
     CDS             6463..6678
                     /locus_tag="VHF88_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2160831.1"
                     /translation="MTSATKSFARRLRQARRSSGMTQHELGARAGISGSQVYRLEAAE
                     REPRLSTLVSLARALGKDPSDMVRGLN"
     gene            6862..7449
                     /locus_tag="VHF88_03330"
     CDS             6862..7449
                     /locus_tag="VHF88_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2160832.1"
                     /translation="MAPDADQLARQLARRSDAELLALTPSVPDAFDVFFVRHSKAIAS
                     YLWRLTGNHEVAADLTSETFAVALESVGRYDPAKGDARGWLFGIARITMLSSFRQQRS
                     ERAARLRLGHSVLGHSDEAWEEAESRIDAALPGLVEDIEHLPRAERRAVVARVLEERE
                     YAEIAASEAATEAAIRQRVKRGLGRLRKRIEGDGR"
     gene            7446..8366
                     /locus_tag="VHF88_03335"
     CDS             7446..8366
                     /locus_tag="VHF88_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160833.1"
                     /translation="MSAARHLLAWLWAAIWLRWRLAQVKLRPLLEGGSALAGTGPDYH
                     LEPAEIPVEGRDYAPDELPADLLAKLEATRLELRGRARRAPSRSGRPMQWRRTAAIVA
                     AALLGLGTVGAGATALVNGSTGVPAVDRLLGLYEAELEEPGSSGRPGGVGSDFEPRSS
                     GSSTSVEVSLGSRQIVSTSYVARDGRVCVALTSGRDDRGDLVCLSESTLATRLDSDGA
                     ILAGILAEPDATILTGFVRGDVTDLVGRGPGGPLEVHLGRPWTPDLLGVGTLRSFLAL
                     GDPGTSGLPDPRDYVIRGQTNDGDTVNVVP"
     gene            8465..8857
                     /locus_tag="VHF88_03340"
     CDS             8465..8857
                     /locus_tag="VHF88_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160834.1"
                     /translation="MRHAKAWRATLAVAAVSALGMAVPAQASAFTSWYSCVSKPSGQW
                     CDGRANGTYDGQHSWDYNSGAPTSGSFYVCQQVYHPASGTSLAGSSCGLDFVSHVYGN
                     VTCVCYDAEVAQTSGSPQSINGFADADF"
     gene            8917..9489
                     /locus_tag="VHF88_03345"
     CDS             8917..9489
                     /locus_tag="VHF88_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160835.1"
                     /translation="MGAIADPDESNLPGAGMPQATEIDPEAREAMAVLDRPREAADAM
                     SPDLAERIDARADFGMNPGLSRLSIGNATNSVYVIPANGHVCAALTVGEGANLSCRSN
                     ETIAGGKAAAATVVLETRDIAIYGIVPDGVESVSVHSGASEPSVVPTEGNAYYTVVPA
                     GTPLKGVSYAGPSGPVEFPLYDPSRIERGS"
BASE COUNT         1540 a         3473 c         3608 g         1466 t
ORIGIN      
        1 atcgccgcca gcgcgaccag cacgagcgcc agcggggtcg tgacgcggtt gaaccacggc
       61 ggcccgacgc tcgcctcctc gcccgacagc gcctcggaga tcagcgggaa gaacgtcccc
      121 cagaagatca ccacgcagag cccgaccagc acgaggttgt tgacgaggaa gaaggcctcg
      181 cgcgacatca gcgagtcgag cttggcgtcg ctgcggaggt cgtcgaggcg cgacacgatc
      241 agcgccgtcg agccgaggat cacgatggcg atgaaggtca ggaactgctt gccgatcgtc
      301 gacgcgccga acgcatggat cgagtcgagg atcccggagc gcacgaggaa cgtgccgagc
      361 agcgcgagca cgaacgtcga gacgatcagc gagacgttcc agaccttcag catcccgcgc
      421 ttctcctgga ccatgatcga gtggatgaac gcggtgccga tcagccacgg catcagcgac
      481 gcgttctcga ccgggtccca ggcccagtag ccgccccagc cgagctcgga gtaggaccag
      541 agcgccccga gcatgatccc ggcgccgagg aaggtccaag cgatcagcgc gaagcggcgc
      601 gtcgcgcgga tccagtcggc gccggtctgg cggacgatca gcgcgccgat cgcgaaggcg
      661 aacgggatcg agaagccgac gtagccggtg tagagcatcg gcgggtggat catcatcgcc
      721 ggatggcgca gcagtgggtt gagccccgca ccctcgaccg gcgcgacggc gagcgtcgtg
      781 aaggggttct cccagccgac catcagcagc aggaagaacg ccgcgatccc gcccagcacg
      841 gcggtcgcgt acggcgcgat gtcacgcagc ccgcgccgcg tcgcgaacag caccgcgctc
      901 gcgaacagcg acatcagcag cgcccagagc agcagcgacc cgtcctgggt cgcccacatc
      961 gcggtgaact tgtagaaggt cggcgtgtcg gtcgaggagc cctcggcgac gagcttgaac
     1021 gagaagtccg agcgcaggaa ggccgactgc aggacgaccg tcgcggtgac catcagagcg
     1081 gcgacgcagt agatcgcgcg ccggccggag atgacggccg cgcggctgtc cgcgcgggcg
     1141 ccgtagagcg aggcgccgat cgcgtagacg gcggtcagca ggccgacgag cagacaggcg
     1201 gaaccgagtc ccgccacctc aggtgctctg ctcgtcctcg gtgaacttcg atgggcactt
     1261 ggtgatcagc gagtcgcgct cggcgacgaa ggtgccgccc tccagctggc ccgagacgat
     1321 cacctcgcgg ccctcgcgga aggggtccgg cacgacgccg gcgtaggtca ccggcaccga
     1381 ctcgcgtccg tcgcgatcgc ggatccggaa cgtcagccgg tcgccgtcct tgtcgaccga
     1441 gtccttgacg accttgccgg tcagctcgta ggagcgaccg ggctcggcac ccgtcaggag
     1501 gtcggacggc tgcttggcct gcgtggacgc gctgaagctc gtgtagacga gcgcgccggc
     1561 cagcaggacg gcggcggaga gcgcgacgaa caggcgcaac ctgcgctttc gactggggtc
     1621 catcgacact gattatgcct agcatgccga gccgatgacc ggtctgaccg cttgaccgca
     1681 ccacaaccaa cgcgccggga ccgggttacc gctgctgtgt cccgtccgca cacgaggtcg
     1741 ccggctgccc tggccgcggc gctgacgctc gccgcgctcc tgttcggtct cgccgtgacc
     1801 ccggcgagtg ccgcaccgcc gcggccgatc gagctgcgct ccggatggga ggtgctcgag
     1861 ggcgactggc gtccgacggc ggtgccgtcg accttcgagg gcctcgcgcg tgcgcccttc
     1921 tacggcggca cgctgaagct ctaccggctg cgcttcacgg cgccggacgt cgagggctac
     1981 gactgggcgt tcgacttcga gcaagttcgc cggcgggcaa ccgtgtcgct caacggccgc
     2041 cggatcggac tcagcatcga cccctacacg ccgttccgag ttcccgctcg cgggctgctg
     2101 ccgggcgagg tgaacacact cgaggtattg gtcgacagtc gcaaggatcc ccgcctgccg
     2161 gagggctggt ggaactgggg cgggatcgtg cggccggtga agatggtgcc gatcggcccg
     2221 gcccacgtgc gggacctggc gctgctgccg tcggtccgct gcagcggtcc cgcgaccgac
     2281 tgcagcgcgt cgctgctgat cgacggcatc cttgagaagc tgccgcgtca gcctccgccc
     2341 agcgaggtcg tcgacgggcg gcgggtcgtc ctgccgccgc cgcgaccgct gctgaagatc
     2401 cgcctggagg cgcccgatgg cgaggtcacc ttccaccgtg cctggctgcg agggtcgagc
     2461 gccggtcgtc gccggcagaa gatccagatc gccatgccgc cgccgcagct gtggtcgccc
     2521 gagctgccgc agctctacga cgcgcgcgtg acgctgaaat acgacggcga ggtgacgcag
     2581 gtcgagaagc gcaagctcgg cttccgctcg atcgaggtcc gcgatgggat gctgctgctc
     2641 aacaaccgcc cgatcaacct gcgcggcgcc tcgatccacg aggacgcgcc aggccgcggc
     2701 gcggcgatca acggctacga catggacacg acggtgcgtg agctgaagga gctcggcgcc
     2761 aacgtgacgc gggcgcacta cgtgctcagc cagggcctgc tgaaccgctt cgacaaggcg
     2821 ggcatcctcg tctacaacca ggctccggtc tggcagcgcg accacggcgc gaacctgttg
     2881 cggcggccga tcgaccgggc ccgtgcggtc gcgcaggtcg agcgcacggt gctcgccgcg
     2941 cgcgggcacc cgtcggtgat cgtccactcg gtcgccaacg agctgacgtt cacgcccgac
     3001 cgccggccgg cgtcgcggcg gttcctgctc gacgccgcga aggcggcgcg cgacctcgac
     3061 ccgctggtgc cgatcgcagt cgacatcaag acccgtccct ggctgccgcg tcagcgcacc
     3121 tacgacgcgt tcgatctgat cggcatcaac cagtacttcg gctggtatcc ctgggtcgag
     3181 gacttcgccg gcctcgagcc gttcctcaac cggatgcgcg cctactaccc cgagcaggcg
     3241 ctcgtgatga cggagttcgg cgccgagggt cggcccgaca tggccggtga cccgatcgac
     3301 gtcaagggca gctacgagtt ccaggccaat cacgtcgcgc gcacgatcga cctggtcgac
     3361 cgtctgccgt tcatgtcggg cgcgatccac tggacgctgc gcgagttcga gatcttcccg
     3421 gactggcgcg gtggcgcctc gggccacgaa ggccccaacg acaacatccg ccatcacaag
     3481 ggcgtcctca cctacaccgg cgagcgcaag ccggcatggc aggtcctgta cgaccgcttc
     3541 gtgcggacgc ctttctaccg ctgagctggg ggctagggct gtagggctga gccgctgaag
     3601 tgcactccgg cggcgcgtag aggggggcag acgctgccgg tcgcgaagcg gcggccgtag
     3661 agctcgccgt cgctcgtcag cacggagcgg gcaccgaggg cctcgacgtg cgagaggatc
     3721 gagagggcgc tgtcggtgat ccgcagctcc tcgagcgggc gggtgatctg cccgtcctcg
     3781 atcaggaagg tcccgtcgcg ggtgacggcg gtgaagcggc tttcggtcgg gcgcagaacg
     3841 ttggtgtacc agaggcgggt gacgtagatg ccgcgctcga ccggggcgca cagatcggtc
     3901 tcatcggccg cgccgccgcc gatcagcacg aggttggtcg ggcgcggtcc ggcagcctgc
     3961 ccgccggcga cgagcgcgtg accggtgctg gcggttgtcc cgccggcgac cgcggcgctg
     4021 cgcgtgtcgt gcacgacacg gtgggcgacg ccgtcctgga tcagtggcaa cggcgccttg
     4081 ggcacgccct cggcgtcgaa ggcgcgcggc agcgtcgcct ggaagcgcgg cgagtccgag
     4141 aggttgatcg cggggcttac gacgcgtgtg ccgagccgct cggtcaacgc gccgcggccc
     4201 tcggcatggg cgaggccgtc gaatgcggtc tcggcgagca cccagagcag gtcgaggacc
     4261 gcatgggcgt ccatcacgac ggtgtagtcg cccggggtca ggctgaccgg tgctccgtcg
     4321 acggcggctt tgacggcggc gcgctcggcc aacgcggcgg gatcgagggc ggccgcggcg
     4381 atcgccgact gcgcggcgta gccggtgcgg ccgtccggcg cggccgcgat caccttcatg
     4441 aaagcgtcgg tcacgcgatc gctcagctcg acgccggtcg acgaggcgat cgccgtctcg
     4501 acgtcgcccg cggtccagat gccgcccagc tcgaccgggt cgtgcggcgc gtcgaaggcc
     4561 gtctgcagcg cctctgcgcc ggtcctcggg tcgagccgcg cggtctcggc gtcgaatcgt
     4621 cgtgggcgcg cggcgtggcg gcacccggaa agccggggta gtgcccctcg cccgcgctat
     4681 ccgccgccgc ctcagccgcc agcgccgccg cgcgagcgca ctcccccagt gcttcgcgat
     4741 gaagccggtt cgtcgccgct cggccgacgt ggccgtggcg caagatcgcg atctccaccg
     4801 tcaggtcgtc gatcgacgtc gcctgcgtcg gccgcgagcg ggcgaagcgc agcatcgccg
     4861 agcgctcgcg ggtgacggtg acgagcgcct cgccgctcgt ctcggcgaga gcacgccgag
     4921 ccgtatcgag cgcctcgctc acccggcgcc gacctggact ccgcgaaatc gcgccggagc
     4981 acaaccgtgg gcgacccgag ctaactgtcc cggctcaccc ttgccgcagt cggtgaacga
     5041 ggtgagcgcc cactcctccg ccgagcagac cgcgtcgagc gagccccaga acttgggcgt
     5101 cacgcctgcg tagacgggat cgcggagcag tcgcccgagc ctgccgtcga cgatctcgcg
     5161 ggccgcctcg ccgccgaact ggaagtgcag ccgccgcgag tcgatcgacc aggaccggtt
     5221 cgtgtcgatg tagatcccgc tctccacgcc gctcagcagg tcggccagcg agccgccctg
     5281 gccggggtcg aggctgacgt tgctcatccg gacgatcggc tggcgggcga atccctcggc
     5341 gcgggcgcag ccgcccgagc gctcgagccc gatctccgca gcgctgccgc ggttcgacag
     5401 gaagccgcgc aggacaccgt cgcggacgat cgatatcgac tgccccggca cgccctcgtc
     5461 gtcccagcga tagctgccga tcgcgcccgg cacggtcgcg tcggccgtga ccgacatcgc
     5521 atcggagccg taacgcagcg cgccgccgcc atcggccggt acccagctcg tccccgcgta
     5581 ggaggcctcg tagccgagca cgcggtcgag ctcgaccgcg tggccaaccg actcatgaat
     5641 ctgcaggccg agctgctcgc cggccagcac gagcgtcgtc gggccctcgg ggcagggtgg
     5701 cgcgtcgagc agcgcgacgg cctcttcggc cacgcgtggc gcgtggccgg gcaggtcgag
     5761 cgcgaggacg tgctcccagc cggcctgggc gacgctcccc gcgtgcgagg ccgggtagga
     5821 gcgcgtctgc gcatcgtcac cgtggacggc ggtcgcgctc acgacgccac cgcagtcggt
     5881 cagctcctgc tcgcagagcg tcccctcggt cgacgccagc accttctgct cgcgtcgcga
     5941 ctgcagcgcc gtgaacgcga cggtcacgcg cggctgctca cgcagcgcgc agtcggtctc
     6001 gtggagcagc gccagcttct cgtcgagtga gacggcaaac gggtcggtgg cgaacggcga
     6061 gacgtggcgg ccggtcgcgg cgggctccgg cgtcagcggc tggctgccca cgcgtggctg
     6121 agcctcggcg atcgcgatcg cgcgggtgag cgcatcctcg gctgccgtcc tgtccgatcc
     6181 cgccgcggcc gcgaagcccc agccgccgcc cagctggacc cttgcgccga agccctcctc
     6241 atcgcgcgcg tcgagctcgt cgagctcgcc gttgcgcatc gcgaccgact cctcgcgcag
     6301 gcgcacgtgt cgcatgtcgg catagtcgac gcggccggcg gccgcgtcca tcaggtcacc
     6361 gaggaggtcg cgcaccgctg cgaccctagc gttagggggt ccttatcaca cgataagggt
     6421 gcagaatgtc acagatcgca ttccggccta caagagcgcg agatgacgtc tgcgaccaag
     6481 agcttcgccc ggcggctgcg gcaggcgcgt aggtcgagcg gcatgacgca gcacgagctg
     6541 ggcgcgcgtg cggggatcag cgggagtcag gtctatcggc tcgaggccgc cgagcgcgag
     6601 ccgcgactct cgacgctggt gtcgctcgcg cgggcactcg gcaaggatcc cagcgacatg
     6661 gtccgcggtc tcaactgagg gagagcttcc cggccggaag ctgcgcaatc gccgtcgcgt
     6721 gtcacaggat cgctgtcgac gtcaagcagg ggatagttcg acggctatca ggagggtggg
     6781 cgcatgccgg ttattggcat gcaagggggg ttgcttacgg aatcgatgca tcgcgcgttc
     6841 cgccgcgatg atgcccgggc gatggcgccg gacgcagacc agctagcgcg ccagctggcg
     6901 cgccgcagcg acgccgagct gctcgcgctg acgccgtcgg taccggatgc cttcgacgtc
     6961 ttcttcgtcc gccacagcaa ggcgatcgcc tcctatctgt ggcgcctgac gggcaaccac
     7021 gaggtcgcag ccgacctgac ctcggagacc ttcgccgtcg cactcgagtc ggtcggccgc
     7081 tacgaccccg cgaaaggcga cgcccgcggc tggctgttcg gcatcgcgag gatcaccatg
     7141 ctgtccagct ttcgccagca gcgcagcgag cgggcggcac gcctgcgcct cggtcacagc
     7201 gtgctcggcc acagcgacga ggcctgggag gaggccgaat cgcggatcga cgcagcgctg
     7261 cctggactgg tcgaggacat cgagcacttg ccgcgtgcgg agcgtcgcgc cgtcgtcgca
     7321 cgcgtgctcg aggagcgcga gtacgccgag atcgccgcga gcgaagcggc gaccgaggcc
     7381 gcgatccgcc agcgcgtcaa gcgtggcctc ggtcgcctgc gcaagcggat cgagggggac
     7441 ggccgatgag cgctgcgcgg catctgctgg cgtggctctg ggccgcgatc tggctgcgct
     7501 ggcggctggc gcaggtcaag ctgcggcccc tgctcgaggg cggttcggcg ctggccggaa
     7561 cggggcccga ttatcacctc gagcctgccg agattccggt cgagggtcgt gactatgcgc
     7621 cggacgagct gccggcggac ctgcttgcga agctggaggc gacgaggctg gagctgcgcg
     7681 gtcgtgcgcg ccgggcgccc tctcgaagcg gtcggcctat gcagtggcgc cgcacggcgg
     7741 cgatcgtggc ggcggccctt ctcgggctcg ggacggtggg agcgggggcg acggcgttgg
     7801 tgaacggctc gaccggggtg cctgcggtcg atcgcctgct ggggctctac gaggccgagc
     7861 tcgaggagcc cggatcgtct ggccgtcccg ggggcgtcgg gagcgacttc gagccccgct
     7921 cgtccggatc gagcacgtcg gtggaggtgt cgttgggctc gcggcaaatc gtcagcacct
     7981 cctacgtcgc ccgtgatggg cgggtgtgcg tggcgttgac gagcgggcgt gacgaccgcg
     8041 gcgacctcgt ctgcctgagc gagtcgaccc tcgcgactcg cctcgacagc gacggagcga
     8101 tccttgccgg aatcctcgcg gagccggacg ccaccatcct cacaggcttc gtgaggggcg
     8161 acgtgaccga cctcgtgggc aggggccccg gaggtcctct cgaggtccat cttggacggc
     8221 cttggacccc tgacctgtta ggtgtgggaa cactgaggtc cttcctggcg ctcggtgatc
     8281 ctggtacctc gggtctcccc gatccgcgcg actacgtaat tcgcggtcag accaacgacg
     8341 gcgacacagt caacgtcgtg ccgtaggcag catgccttga cgcgcgtccg acggccggtc
     8401 tatggtcgcc gtggcatctc gctgggtgcc ggttggactc gctaatcgag aggggttgga
     8461 atatatgaga cacgcgaagg cgtggcgagc gacgcttgcc gtggccgctg tcagcgcact
     8521 cgggatggca gttccggcac aggccagtgc ctttacgagc tggtacagct gcgtgagcaa
     8581 gccgtccggc cagtggtgcg acggtcgcgc gaacggcacc tatgacggcc agcacagctg
     8641 ggactacaac tcgggggccc cgaccagtgg ctccttctac gtgtgccagc aggtctacca
     8701 ccctgcgtcg ggcacctcgc tcgcggggag ctcttgcggt ctcgacttcg tatcgcatgt
     8761 ctacggcaac gtgacatgcg tctgctacga cgcggaggta gcgcagacga gcggtagccc
     8821 ccagtccatc aacggcttcg cggacgcgga cttctaatgc gtacgcgagc gcggtaccta
     8881 ccattcgtca tcgtcgtctg cctgctcgtg gcatcggtgg gagcgatcgc cgatcccgac
     8941 gagagcaacc tgcctggggc cgggatgcca caggcaacgg aaatcgatcc cgaggccaga
     9001 gaggcgatgg cagtcctcga ccggcctcgc gaggccgcgg acgccatgtc gccggacctg
     9061 gcggagcgga ttgatgcccg ggccgacttc ggcatgaatc ccggcctctc gcggctctcg
     9121 atcggcaatg cgacgaactc tgtctatgtc atccctgcga acgggcacgt ctgtgctgcg
     9181 ctcacggtcg gggagggcgc gaacctgtcc tgccggtcca acgagacgat tgccggcggc
     9241 aaggctgccg cggcgaccgt ggtgctcgag acccgggaca tcgcgatcta cggcatcgtc
     9301 ccggacgggg tcgagtccgt ctcggtccac agcggggcgt ctgagccgag tgtcgtgccg
     9361 accgagggca atgcgtacta cacggtcgtt ccagccggga cgccgctcaa gggggtcagc
     9421 tacgcgggtc cgagcggtcc cgtcgagttc cccttgtatg acccgtcgcg catcgagcgc
     9481 ggatcctagg ggcgtcggcc catatgggct actagacaag aggtcagcgc tccggtaatt
     9541 tgccaactgg cgaatggagg gcgcaaatcg cagcggaagg cggcaccggt cgcggaggag
     9601 gatctcctcg ccggtcgccg cctccggtgc atctgcgccc gaagtttcgt tcgccgaccc
     9661 ggcgtggtcg aaacgccagg tcactaccgg catgccaccg gagtctgcgc ggatcgccct
     9721 caaccccggc ggttccgcgc cagcgagacc aggttgcatc agctaacgct ctccggcgac
     9781 gtcccggccg gctttcggcc gatcgtcgcc gggggccggt aggcctcatg tcacagacat
     9841 ggccgtactc tacgagtagc cgtagcggcc acggcaccga tgcttggggg gactcaacag
     9901 cgcatcggcg cccgaaggcc cgcgacgccg atgcttatga ccgactccat cgacatgccc
     9961 ggcggaacct ttggggacaa cctcaaggag gcgcgcctga ggcgcggcat gtcgcacgag
    10021 gagctcagtg cccgagccgg catcgacatc gccaccgtct atcgcctcga ggctggcgat
    10081 cgccagc
//
LOCUS       DATABV010000081         9380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1149146, whole genome shotgun sequence.
ACCESSION   DATABV010000081 DATABV010000000
VERSION     DATABV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149146"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VHF88_03350"
     CDS             <1..711
                     /locus_tag="VHF88_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX2160836.1"
                     /translation="AGGLGAPAAFYLAAAGVGTLGIVDDDVVDLSNLQRQIVHTVDRV
                     GMPKVDSAEQAIHELNPGVEVVKYKTRLDASNIMEIVEGYDIVVDGVDNFPTRYLLND
                     ATVRLKIPVVSASILGFDGQLSVFAPYDGPCYRCLYPTPPPAELAPSCGANGVLGALP
                     GIMGVLQAIEVVKLITGSGEPMIGRLLLYEALGTSFTDLKVRRDPDCPICSRDPDSIS
                     DDEMGFFPDYEQFCAAAG"
     gene            728..1003
                     /locus_tag="VHF88_03355"
     CDS             728..1003
                     /locus_tag="VHF88_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX2160837.1"
                     /translation="MSTIKIPPVLRPSTGGEREVSAAGADVGEVLRALAEQHPATQSQ
                     LFGEDGALNRYVNVYLNDEDVRVLDGLDTAVKDDDTLVILPAMAGGA"
     gene            1055..2029
                     /locus_tag="VHF88_03360"
     CDS             1055..2029
                     /locus_tag="VHF88_03360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX2160838.1"
                     /translation="MAEGLQNRPCGGRYGDIVAAIGNTPLVELKRLSPKPGVRIYAKL
                     ESYNPTGSVKDRVARGLIEDAEARGAIAPGQTILEPTSGNTGISLAMICQRKGYPLKV
                     VMPDNVTEERTQLLTMYGAEIVYSPGAQGSNGAVALALELAESDSSFYMPYQYGNEAN
                     PNAHYNGTAVEILEELDEVSAFVAGLGTGGTLMGNGRRFKEHDPDTVVVAAEPMQGEL
                     VQGLRSLDDGFIPPIIDLALIDRKIFVSNRDAVVWTKRVLAEEGLFLGVSTGAIAAIA
                     VRIAGELDEGNVVFVAPDDGWKYLSSGVYSKPIEQLEAEGVLDSASFW"
     gene            complement(2106..2441)
                     /locus_tag="VHF88_03365"
     CDS             complement(2106..2441)
                     /locus_tag="VHF88_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2160839.1"
                     /translation="MAFLAPSPMELVVILVIALLVLGPSRLPETARALGRGMRELRDS
                     LQGIGQDDEDDDDGLEIEPPAPRRDDPGGLPVDPAVVMGDVPYDDDLPDDLYTPRNGD
                     DSEPAQTKT"
     gene            2514..2900
                     /locus_tag="VHF88_03370"
     CDS             2514..2900
                     /locus_tag="VHF88_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX2160840.1"
                     /translation="MRMTRAQWDALIAHAREEAPNECCGYARARDGVIDEVYRAVNER
                     RSPYGYEFGFEALKATNELDDEGWEVATYHSHPRSPAEPSQTDINLATYPEWTYLIVS
                     LDGEPHVRAWRIAEGRVDEQPIEIDG"
     gene            2893..3327
                     /locus_tag="VHF88_03375"
     CDS             2893..3327
                     /locus_tag="VHF88_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160841.1"
                     /translation="MAELPGPGQGGEEQVEPARSEAHERARKINPAYAEGELIKVGWA
                     GNQAEAELIQGLLLENGIPSSLRRSMGFDVPDFLAAGPRDIYVPASGAEVARGLLDDT
                     GMARPSERDEGGPRLGQALKIAAVLVLVGGGVVLLAWLLQPG"
     gene            3410..4609
                     /locus_tag="VHF88_03380"
     CDS             3410..4609
                     /locus_tag="VHF88_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2160842.1"
                     /translation="MDFAPSERVAELRDRVRGFMAEHVMPVEEEAMRALDEEVGPGVA
                     YPAVLVELRERAKREGLWNLFMPDEEHGPGLANWEYGLLCEEMGRSPVAPLAFNCAAP
                     DTGNMEILAEYGTPEQKERWLAPLLSDHVRSCFSMTEPETAGSDPTGLAARAVLDDGE
                     WVIDGHKWFTSGYNGADVTIAMVVTDPDAAPHRRASMILVPIDTPGFSGVRPLPVMGH
                     SAGPGHWEVRYEGVRVPEANLLGPRGDGFAIAQARLGPGRIHHCMRAIGSAERAFELM
                     CRRANEREAFGTPLGDKQFVQEFVATSRIEIDAARLMVLHAAWKMDSEGKRAARQQIS
                     MIKVVAAQMHQAVCDRAMQVHGALGMSDDVPLALMWRQGRWLRIADGPDEVHKQSIAR
                     RELSRFK"
     gene            4652..5818
                     /locus_tag="VHF88_03385"
     CDS             4652..5818
                     /locus_tag="VHF88_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2160843.1"
                     /translation="MATAAPTSPADHGLSDEQRDFVAAIRDFCTREVTPEKLDALTDG
                     GKDPHNHAVAERMGELGWYGLTIPEQYGGSGGTFFDATLFLEEVARGQAPVGAYGVTL
                     ICVGALNRFGSEQQKQDLLGGVARGGTLAIAMSEPDAGSDVAALKCRARLDGDEWVLS
                     GSKMWCSFAHKASHVLIVCRTGEGSERHEGLSMIWVPRDAEGFTMRPIDTLGGEDTNE
                     LHLDDVRVPAANLLGNEGGGWIQLMAGLNNERVILAALALGLAQRAFDDTLAYVKQRH
                     QFGRPVGSFQALQHRLADLATDLAQARLLIRWVARLTDEDPGRMLPQEASMAKLAATE
                     LAKRCALEAVQMAGGYGYAKEYPMERHLRSAVVTTIYGGTSEIQRNIIAKTLGL"
     gene            5976..8645
                     /locus_tag="VHF88_03390"
     CDS             5976..8645
                     /locus_tag="VHF88_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2160844.1"
                     /translation="MRAVTRLLSAAADLQGPDVVRRRLVAEARDFFGVARAVLLEIHE
                     SDGRAEPVVASPTIGVARRPLAIAELPPLELLLRQGLPALLAQRDEAHEIDRLLGADG
                     SAGTALLLPMRAGSAVRNVLLLLDDDGRSFSEVEIGVAGSFAAAAAASLAQMRLAEEH
                     AAQVAQQAALARAAKTLNESLDLNRVLVQICREAASILDGDNAAIFRGDANGLTIEAV
                     YGIVPEAIGYRVAPGVGLSGRVAELDRPLITNDYQQLTPKPDAAFFGDVRSCVAVPMH
                     WDGKLRGVLAVGFHRRREVTREDMNLLEAFGELAAAACRNASAHAGLAQVARTDGLTG
                     CLNHTAMQHMLRREIERGERTGHRLSLILIDLDDFKQVNEEHGHLVGDEVLRRVGAAL
                     RAAVRPYDLVARYGGDEFAIVAIEADERAAAELARRTLDGIGRAFEAEGDAPDGCGAS
                     AGVAEWEPDQTPAALIDNADRALLHGKQHRGRGSVVVYSDLPAGLRPLPPRRPETAQP
                     AFAPMAPVEAAWPDQGREQTERLRLRTRQLSLANAIGTRLSAMTGPEEIIDAAVDELN
                     RAFGYFLCAIVRIREDNYVEAVASRGTAQSHDETRPPAMPRAAGVIGRCLRERRPVIV
                     GDVRDEPDYVAQPKTSGVRSELAVPIWVGDDLWGAIDIEEERPDAFDEDDARLVQTVA
                     GQVGSALRSAMLYEQLDGAYIGTAQALAAALEAGDRGEGAAGPLIELVTAVGRRLDLS
                     DDAIRSLRLAAIFHDIGKVSVSQDVLNKRGPLTDSERLQVERHTVVGESILSSVEFLE
                     DVRPLVRHAHERWDGGGYPDGLAAEAIPLGARIVHACDAYDAMTAERPYRKPLSDADA
                     RSQLVAGAGSQFDPGVVAALLAVLDAELPAATA"
     gene            complement(8642..9268)
                     /locus_tag="VHF88_03395"
     CDS             complement(8642..9268)
                     /locus_tag="VHF88_03395"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX2160845.1"
                     /translation="MPIAFKEWAVTVRALAEGEQLVTVRKGGIREENKHFEIEHDRFF
                     LYPTFDHQQGQLVRDSHRPELGRALEEGVWADGEPHPSVLMNGGVTQPDRVRIRAWAE
                     VAASYLITDPRAVDALSPYYVWTTDYAEKRLEWKRRHPMHVILLRTYRIPRPVTVKVR
                     PEYHGCRSWIELHRDLPFEGTPVLSDEEFDRAAAEIEAIASDAVPVLA"
BASE COUNT         1393 a         3328 c         3363 g         1296 t
ORIGIN      
        1 gcgggcgggc tcggcgcccc ggccgccttc tacctcgcgg ccgccggcgt cggaacgctc
       61 ggcatcgtcg acgacgacgt cgtcgacctg tcgaacctcc agcgccagat cgtccacacc
      121 gtcgaccgcg tcggcatgcc gaaggtcgac tccgctgagc aggcgatcca cgagctcaac
      181 ccgggcgtcg aggtcgtcaa atacaagacc cgcctcgacg cgtcgaacat catggagatc
      241 gtcgagggct acgacatcgt tgtcgacggc gtcgacaact tcccgacgcg ctacctgctc
      301 aacgacgcca ccgtgcggct gaagatcccc gtcgtctcgg cgtcgatcct cggcttcgac
      361 ggccagctgt cggtcttcgc cccctacgac ggcccctgct accgctgcct ctacccgacg
      421 ccgccgccgg ccgagctggc gccctcctgc ggcgccaacg gcgtgctcgg cgcgctgccg
      481 gggatcatgg gcgtgctgca ggcgatcgag gtcgtcaagc tgatcaccgg ctccggcgag
      541 ccgatgatcg ggcgcctgct gctctacgag gcgctcggca cgagcttcac cgacctcaag
      601 gtgcgacgcg accccgactg cccgatctgc tcacgcgacc cggactcgat ctccgacgac
      661 gagatgggct tcttcccgga ttacgagcag ttctgcgctg cggccggctg agatagggtc
      721 acccgctgtg agcacgatca agatcccccc ggtcctgcgc ccctcgacgg gcggggagcg
      781 cgaggtgagc gccgccggcg ccgacgtcgg cgaggtgctg cgggcgctcg ccgagcagca
      841 cccggcgacg cagagccagc tgttcggcga ggacggcgcg ctcaaccgct acgtcaacgt
      901 ctacctcaac gacgaggacg tgcgggtgct cgacgggctc gacaccgcgg tcaaggacga
      961 cgacacgctc gtaatcctcc ccgcgatggc cggcggcgcc tgaggccgaa gggcctaggc
     1021 gggtcttgca cccgtagcta cactttttga agcgatggcg gagggtctac agaaccggcc
     1081 ttgcggcgga cgctacggcg acatcgtcgc ggcgatcggc aacacgcccc tggtcgagct
     1141 caagcgcctc tcgccgaagc ccggcgtgcg gatctacgcg aagctcgagt cctacaaccc
     1201 gaccggatcg gtcaaggacc gcgtcgcgcg cgggctgatc gaggacgcgg aggcgcgcgg
     1261 ggcgatcgcg ccgggacaga cgatcctcga gccgacctcc ggcaacaccg ggatctcgct
     1321 ggcgatgatc tgccagcgca agggctaccc gctcaaggtc gtgatgcccg acaacgtcac
     1381 cgaggagcgg acccagctgc tgacgatgta cggggccgag atcgtctact cgcccggcgc
     1441 tcagggctcc aacggcgcgg tcgcgctggc gctcgagctg gccgagtccg attcctcgtt
     1501 ctacatgccc taccagtacg gcaacgaggc caacccgaac gcccactaca acggtacggc
     1561 ggtcgagatc ctcgaggagc tcgacgaggt ctcggcgttc gtcgcggggc tcggcaccgg
     1621 cggcacgctg atgggcaacg gacggcgctt caaggagcac gacccggaca ccgtcgtcgt
     1681 cgccgcggag ccgatgcagg gcgagctcgt ccagggcctg cgctcgctcg acgacggctt
     1741 catcccaccg atcatcgacc tggcgctgat cgaccgcaag atcttcgtct cgaaccgcga
     1801 cgccgtcgtc tggaccaagc gcgtgctcgc cgaggagggg ctgttcctcg gcgtctcgac
     1861 cggggcgatc gccgcgatcg cggtgcggat cgcgggcgag ctcgacgagg gcaacgtcgt
     1921 cttcgtcgcg cccgacgacg gctggaagta cctcagctcg ggcgtctaca gcaagccgat
     1981 cgagcagctc gaggcagagg gcgtgctcga ctccgcatcg ttctggtagc tgcggtgctc
     2041 gtcggacctc gctcccgcga gctacgaggc gtcgctgtag gaccggtccg cctgcgctcc
     2101 tccgctcagg tcttcgtctg ggccggctcg gagtcgtcgc cgttgcgcgg cgtgtagagg
     2161 tcgtcgggca gatcgtcgtc gtaggggacg tcgcccatga cgacggctgg gtcgacgggt
     2221 aggccgccgg gatcgtcgcg tcgcggggcg ggcggctcga tctcgagccc gtcgtcgtcg
     2281 tcctcgtcgt cctggccgat gccctgcagc gagtcgcgca gctctcgcat cccgcggccg
     2341 agcgcgcggg cggtctcggg cagccgcgag gggccgagga ccagcagcgc gatcacgagg
     2401 atcacgacca gctccatcgg tgagggggcg aggaacgcca tcgccacaag ggtagaccga
     2461 ccgtgcgcgc ccggcagcgc tgggcgcctg cggctggcca ttacgctgag gccatgcgga
     2521 tgacgagggc ccagtgggac gcgctgatcg cgcacgcccg ggaggaggcg ccgaacgagt
     2581 gctgcggcta cgcgcgcgcc cgcgacggcg tcatcgacga ggtctaccgc gctgtcaacg
     2641 agcgccggtc gccctacggc tacgagttcg gcttcgaggc gctcaaggcg accaacgagc
     2701 tcgacgacga gggctgggag gtcgcgacgt accactcgca cccgcgcagc ccggccgagc
     2761 cgtcgcagac cgacatcaac ctcgcgacct atccggagtg gacctacctg atcgtgtcgc
     2821 tcgacggcga gccgcacgtg cgggcgtggc gcatcgccga gggtcgggtc gacgagcagc
     2881 cgatcgagat cgatggctga gctgcccggt ccggggcagg gaggggagga gcaggtcgag
     2941 cccgcgcgca gcgaggcgca cgagcgcgcg cgcaagatca acccggccta tgccgagggc
     3001 gagctgatca aggtcggctg ggcgggcaac caggccgagg ccgagctgat ccaggggctg
     3061 ctgctcgaga acggcatccc gagctcgctg cgccggtcga tgggcttcga cgtgccggac
     3121 ttcctcgcgg ccggcccgcg cgacatctac gtgccggcga gcggagccga ggtcgcacgc
     3181 ggcctgctcg atgacaccgg catggcacgg ccgagcgagc gcgacgaggg cggcccgcgg
     3241 ctcggccagg cgctgaagat cgccgccgtg ctggtgctcg tcggcggcgg cgtggtgctg
     3301 ctcgcgtggc tgctccagcc cggctgaaag ggattcgcac cgggggctcg cgcccccccg
     3361 gacccccgga gatgcctggc cggtccgcgg cgggtagcct gcgggatcca tggactttgc
     3421 accgtccgag cgcgtcgcgg agctgcgcga ccgcgtccgt ggcttcatgg ccgagcacgt
     3481 gatgccggtc gaggaggagg cgatgcgggc gctcgacgag gaggtcggcc ccggcgtcgc
     3541 ctacccggca gttctcgtcg agctgcgcga gcgggctaag cgcgaggggc tctggaacct
     3601 cttcatgccc gacgaggagc acgggccggg cctcgccaac tgggagtacg gactgctctg
     3661 cgaggagatg ggccgctcgc cggtggcgcc gctggcgttc aactgcgcgg cgcccgacac
     3721 cggcaacatg gagatcctcg ccgagtacgg cacgcccgag cagaaggagc gctggctcgc
     3781 cccgctgctc tccgaccacg tgcgcagctg cttctcgatg accgagcccg agaccgccgg
     3841 atccgacccg accggcctcg ccgcgcgcgc ggtcctcgac gacggcgagt gggtgatcga
     3901 cggccacaag tggttcacct cgggctacaa cggggccgac gtcacgatcg cgatggtcgt
     3961 caccgatccg gacgccgcgc cgcaccggcg cgcgagcatg atcctcgtcc cgatcgacac
     4021 gcccggcttc agcggagtgc gcccgctgcc ggtgatgggc cacagcgccg gcccgggtca
     4081 ctgggaggtc cgctacgagg gcgtccgcgt ccccgaggcc aacctgctcg ggccgcgcgg
     4141 cgacggcttc gcgatcgccc aggcgcggct cggtcccggc cgcatccacc actgcatgcg
     4201 cgcgatcggt tcggcagagc gtgccttcga gctgatgtgc cggcgcgcca acgagcgcga
     4261 ggccttcggc acgccgctgg gggacaagca gttcgtccag gagttcgtcg cgacctcgcg
     4321 gatcgagatc gacgccgcac ggctgatggt cctgcacgcc gcctggaaga tggacagtga
     4381 gggcaagcgc gcggcgaggc agcagatctc gatgatcaag gtcgtcgccg cccagatgca
     4441 ccaggcggtc tgcgaccggg cgatgcaggt gcatggggcg ctcgggatgt cggatgacgt
     4501 cccgctcgcg ctgatgtggc ggcaggggcg ctggctgcgg atcgccgacg ggcccgacga
     4561 ggtccacaag cagtcgatcg cgcggcgcga gctgagccgc ttcaaatgag gctcaccgac
     4621 aggtgaggtc caggggagga acgaggcacc gatggcgaca gcagcaccga ccagtccggc
     4681 cgaccacggc ctgagcgatg agcagcgcga ctttgtcgcg gcgatccgcg acttttgcac
     4741 gcgcgaggtc acgcccgaga agctcgacgc gctgaccgat ggcggcaagg acccccacaa
     4801 ccacgcggtc gccgagcgga tgggcgagct cggctggtac ggcctgacga tccccgagca
     4861 gtacggcggc tccggcggga ccttcttcga cgcgacgctg ttcctcgagg aggtcgcccg
     4921 cggtcaggcc ccggtcggtg cctacggcgt cacgctgatc tgcgtcggcg cgctcaaccg
     4981 cttcggcagc gagcagcaga agcaggactt gctcggcggg gtggcgcgcg gcgggacgct
     5041 ggcgatcgcg atgtccgagc cggacgccgg ctccgacgtc gccgcgctga agtgccgcgc
     5101 ccgcctcgat ggcgacgagt gggtcctcag cgggtcgaag atgtggtgct ccttcgcaca
     5161 caaagcgagc cacgtgctga tcgtctgccg caccggcgag ggctcggagc gccacgaggg
     5221 cttgtcgatg atctgggtcc cgcgcgacgc cgagggcttc acgatgcggc cgatcgacac
     5281 gctcggcggc gaggacacca acgagctgca cctcgatgac gtgcgtgtgc ccgcggccaa
     5341 cctgctcggc aacgagggcg gcggctggat ccagctgatg gccggcctca acaacgagcg
     5401 cgtgatcctc gccgcgcttg cgctcgggct cgcccagcgg gcattcgacg acacgctcgc
     5461 ctacgtcaag cagcggcatc agttcgggcg gcccgtcggc tccttccagg cgctccagca
     5521 ccgcctcgcc gatctcgcca ccgacctggc ccaggcacga ctgctgatcc gctgggtcgc
     5581 acgcctgacc gacgaggacc ccggccggat gctgccgcag gaggcttcga tggcgaagct
     5641 cgccgcgacc gagctcgcca agcgctgcgc cctggaggcg gtgcagatgg cgggcggcta
     5701 cggctacgcg aaggagtacc cgatggagcg ccacctgcgc tcggcggtcg tcaccaccat
     5761 ctacggcggc acgtcggaga tccagcgcaa catcatcgcc aagaccctcg gtctgtaagc
     5821 ccggctggac ggatcgctca cagccacttg gcatagccac gctaaagctg catcggcccg
     5881 cggccgatca ctggttcgtg ggcacccctc gccctgtcac ccggctggcc cctgtagggg
     5941 aaagcgatgc tcgcgtcggc tcggctaccg acgccatgcg ggcggtcacc cgcctgctga
     6001 gcgccgccgc agacctgcag gggccagacg tggtccgtcg ccggctggtc gccgaggcgc
     6061 gcgacttctt cggcgtcgcg cgcgccgtcc tgctcgagat ccacgagagc gacggacgtg
     6121 cggaaccggt cgtcgccagt ccgacgatcg gcgtcgcacg ccgcccgctg gcgatcgccg
     6181 agctgccgcc gctcgagctg ctgttgcgcc agggcctgcc ggcgctactg gctcagcgtg
     6241 acgaggcgca cgagatcgac cgcctgctcg gcgccgacgg cagcgccggg acggcgctgc
     6301 tgctgccgat gcgcgccggc agcgccgtcc gcaacgtgct gctgctgctc gacgacgatg
     6361 ggcgcagctt cagcgaggtc gagatcggcg tcgccggctc gttcgccgcg gcggccgccg
     6421 cgagcctggc gcagatgcga ctcgccgagg agcacgccgc ccaggtcgcc cagcaggcgg
     6481 cgctcgcgcg cgcggccaag acgctgaacg agagcctcga cctcaaccgc gtcctggtcc
     6541 agatctgccg cgaggcggcg agcatcctcg acggcgacaa cgccgcgatc ttccgcgggg
     6601 acgcgaacgg cctcacgatc gaggccgtct acgggatcgt gcccgaggcg atcggctacc
     6661 gcgtggcgcc gggcgtcggt ctctccggtc gcgtcgcgga gctcgacagg ccgctgatca
     6721 ccaacgacta ccagcagctg acgccaaagc ccgatgccgc cttcttcggc gacgtgcgca
     6781 gctgcgttgc cgtgccgatg cactgggacg gcaagctgcg cggcgtgctc gcggttgggt
     6841 tccaccgccg gcgcgaggtc acgcgcgagg acatgaacct gctcgaggcc ttcggcgagc
     6901 tggccgccgc ggcctgtcgc aacgccagtg cccacgcggg cctggcgcag gtcgcgcgca
     6961 ccgacggcct caccggctgc ctcaaccaca ccgcgatgca gcacatgctg cgacgcgaga
     7021 tcgagcgcgg cgaaaggact ggccatcggc tgtcgctgat cctgatcgac ctcgacgact
     7081 tcaagcaggt caacgaggag cacggccacc tcgtcggcga cgaggtgctg cgtcgggtcg
     7141 gcgccgctct gcgtgccgcc gtgcggccct acgacctggt cgcccgctac ggcggcgacg
     7201 agttcgcgat cgtcgcaatc gaggcggacg agcgcgcggc cgccgagctc gcgcgccgca
     7261 cgctcgacgg catcggtcgc gcgtttgagg ccgagggcga cgccccggac ggctgcggcg
     7321 ccagcgcagg cgtcgccgag tgggagccgg accagacccc ggcagccctg atcgacaacg
     7381 ccgaccgcgc gctgctgcat ggcaagcagc accggggacg cggctccgtc gtcgtctact
     7441 ccgaccttcc cgccgggctg cgaccgctgc cgccgcgtcg gcccgagact gcgcagccgg
     7501 cgttcgcgcc gatggcgccc gtcgaggccg catggccaga ccagggccgc gagcagaccg
     7561 agcggctgcg gctgcggacg cgccagctct cgctcgccaa cgcgatcggc acgcgcctgt
     7621 cggcgatgac cggtcccgag gagatcatcg acgcggcggt cgacgagctc aatcgcgcct
     7681 tcggctactt cctctgcgcg atcgtccgga ttcgcgagga caactacgtc gaggccgtcg
     7741 cctcgcgcgg gaccgcgcag tcgcacgacg agaccagacc gccggcgatg ccgcgcgcgg
     7801 ccggcgtcat cggtcgctgc ttgcgtgagc gtcggccggt gatcgtcggc gacgtgcgcg
     7861 acgagcccga ctacgtcgcc cagcccaaga cctccggcgt ccgttccgag ctggccgtac
     7921 cgatctgggt tggcgacgac ctctgggggg cgatcgacat cgaggaggag cggccggacg
     7981 ccttcgacga ggacgacgcg cggctggtcc agaccgtcgc tggccaggtc ggctcggcgc
     8041 tgcgctcagc gatgctctac gagcagctcg acggcgccta catcgggaca gcccaggcgc
     8101 tcgccgccgc acttgaggcc ggtgaccggg gggagggggc ggccggtccg ctgatcgagc
     8161 tggtcactgc ggtcggacgg cggctcgacc tgtccgacga cgcgatccgg tcgctgcgcc
     8221 tagccgcgat cttccacgac atcggcaagg tctcggtctc gcaggacgtg ctgaacaagc
     8281 gcggcccgct gaccgacagc gagcggctcc aggtcgagcg ccacacggtc gtcggcgaga
     8341 gcatcctgtc ctcggtcgag ttcctagagg acgtgcgccc gctcgtccgc cacgcccacg
     8401 agcgctggga cggcggcggc tacccggacg ggctcgccgc cgaggcgatc cccctcggcg
     8461 cgcgcatcgt ccatgcctgc gacgcctacg acgcgatgac cgccgagcgg ccctaccgca
     8521 agccgctgtc cgacgccgac gcgcgctcgc agctggtcgc gggtgcgggc agccagttcg
     8581 accccggcgt cgtcgccgcc ctgctggcgg tgctcgacgc cgagctgcca gcggctacgg
     8641 cctaggcgag tacggggacg gcgtccgagg cgatcgcctc gatctccgcg gcggcacgat
     8701 cgaactcctc gtcggagagc accggggtgc cctcgaacgg gaggtcgcgg tgcagctcga
     8761 tccacgagcg gcagccgtgg tactccggcc gcaccttgac ggtcacgggg cgcgggatcc
     8821 ggtaggtgcg cagcaggatc acgtgcatcg gatgacgccg cttccactcg agccgcttct
     8881 cggcgtagtc ggtggtccac acgtagtacg gcgacagcgc gtcgacggca cgcggatcgg
     8941 tgatcaggta gctcgccgcc acctcggccc aggcgcggat ccggacgcgg tcgggctgcg
     9001 tgacgccgcc gttcatgagc acgctcgggt ggggctcacc gtcggcccac acgccctcct
     9061 cgagcgcacg gccgagctcc gggcggtgcg agtcgcgcac gagctggccc tgctggtggt
     9121 cgaaggtggg atagaggaag aagcggtcgt gctcgatctc gaagtgcttg ttctcctcgc
     9181 ggatgccgcc cttgcgcacg gtgacgagct gctcgccctc ggcgagcgcg cgcacggtga
     9241 cagcccattc tttgaaagca atcggcatgg cgttgaaggg ggtactcggt cgcgccgggg
     9301 gatcggtcct tcgggcgcga ggcgcggatc ttacgcgcag tcgccgggga ggtcaagacc
     9361 cgcgctggac acatgttcag
//
LOCUS       DATABV010000082         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2675935, whole genome shotgun sequence.
ACCESSION   DATABV010000082 DATABV010000000
VERSION     DATABV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675935"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VHF88_03400"
     CDS             <1..369
                     /locus_tag="VHF88_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160846.1"
                     /translation="AAAAPPPPRPVVEAFEANQTVALLFVRKGGIDDRLVKRAVTPLR
                     ALPGVATFVVPAGRIARYAAIAQGVAVDRVPALVVLRPKRLGRSIPTASVSYGFQSPQ
                     SVVQAVVDAGYKGPTVAYHP"
     gene            374..>1921
                     /locus_tag="VHF88_03405"
     CDS             374..>1921
                     /locus_tag="VHF88_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX2160847.1"
                     /translation="MDARDQPSHLRPVPDSGDSTAPALEPQDGATPGLTPPQARGRSS
                     GFVTDVLVELGFVTAEQARQAIELARTAGRPPERLLVEQGSITADQLSRAVAERYGLD
                     HVDLSAYQVDMAAANLISVNTARRYRALPVGFVDKETLLVAMADPTNVLAVDDIQIAT
                     ALDCRVAVAAEEDIESMIGRLNTLQSAVSEAVTEGEAAEEAELAEVADIEVSAEDAPV
                     IKLVYSILGQAVGEGASDVHFEPDEGEMRVRFRIDGVLREAAHVPKRMINAVVSRVKI
                     MSDLNIAEKRVPQDGRVSVTVEDRRVDLRVTTLPTQRGEGATIRILDKDNAQIGLDEL
                     GMDGTARERFEDAFKQAYGAVLVTGPTGSGKSTTLYAALQQLNQVEKNIITIEDPVEY
                     RLSGINQINVNRKAGLDFATGLRSMLRADPDIVMVGEIRDGETARIAIEAALTGHMML
                     TTLHTNDAPGAVTRLTKMGIEAFMIASAVDCVVAQRLARKLCTHCKQRAIVPPEALAD
                     ADIRVGGE"
BASE COUNT          314 a          723 c          648 g          236 t
ORIGIN      
        1 gctgcggccg cgccgccacc gccgcgcccc gtcgtcgagg ccttcgaggc caaccagacc
       61 gtcgccctgc tcttcgtccg caagggcggc atcgacgacc gcctcgtcaa acgggccgtc
      121 acgccgctgc gggccctgcc cggcgtcgcc accttcgtcg tccccgccgg ccgcatcgcc
      181 cgctacgccg cgatcgctca gggcgtcgcc gtcgaccgcg tcccggcgct ggtcgtgctg
      241 cgcccgaagc gactcggcag gagcatcccg accgcctccg tcagctacgg cttccagagc
      301 ccgcagagcg tggtccaggc ggtcgtcgac gccggctaca agggccccac ggtcgcctac
      361 cacccatagg cgcatggacg cgcgggacca accgagccac ctgcgaccgg tccccgactc
      421 cggcgactcg accgcgccgg cgctggagcc gcaggacggg gccacgcccg gcctgacccc
      481 gccgcaggcg cgcggccgct cgagcggctt cgtcaccgac gtcctggtcg agctcggctt
      541 cgtcaccgcc gagcaggcac ggcaggcgat cgagctggcc cgcaccgccg gccgcccgcc
      601 ggagcggctg ctggtcgagc aggggtcgat caccgcggac caactctccc gcgcggtcgc
      661 cgagcgctac ggcctcgacc acgtcgacct ctccgcctac caggtggaca tggccgcggc
      721 gaacctgatc tccgtcaaca cggcgcgccg ctaccgggcg ctgccggtcg gcttcgtcga
      781 caaggagacg ctgctggtcg cgatggccga cccgaccaat gtgctggcgg tcgacgacat
      841 ccagatcgcc acggcgctcg actgccgcgt cgcggtcgcc gccgaggagg acatcgagtc
      901 gatgatcggc cgcctcaaca cgctgcagag cgcggtcagc gaggcggtga ccgaaggaga
      961 agcggccgag gaagccgagc tcgccgaggt tgccgacatc gaggtcagcg ccgaagacgc
     1021 gccggtcata aagctcgtct acagcatcct cggccaggcg gttggggagg gagcctcaga
     1081 cgtgcacttc gagcccgacg agggagagat gcgggtccgc ttccgcatcg acggcgtgct
     1141 gcgcgaggcc gcccacgtgc cgaagcggat gatcaacgcc gtcgtctccc gggtgaagat
     1201 catgagcgac ctcaacatcg ccgagaagcg ggtgccgcag gacggccgcg tcagcgtcac
     1261 ggtcgaggac cgccgtgtcg acctccgcgt caccaccctg ccgacccagc gcggcgaggg
     1321 agcgacgatc cgcatcctcg acaaggacaa cgcccagatc ggcctcgacg aactggggat
     1381 ggacgggacg gcacgggagc gcttcgagga cgccttcaag caggcctacg gcgcggtgct
     1441 ggtcaccggc ccgaccggct cgggtaagag caccacgctg tacgcggcgc tgcagcagct
     1501 gaaccaggtc gagaagaaca tcatcacgat cgaggacccg gtcgagtacc ggctctccgg
     1561 catcaaccag atcaacgtca accgcaaggc ggggctcgac ttcgcgaccg gcctgcgctc
     1621 gatgctgcgc gccgaccccg acatcgtgat ggtcggcgag atccgcgacg gcgagacggc
     1681 gcggatcgct atcgaggccg cgctgaccgg tcacatgatg ctgaccaccc tgcacaccaa
     1741 cgacgccccg ggcgcggtca cgcgcctgac caagatggga atcgaggcct tcatgatcgc
     1801 ctcggccgtc gactgcgtcg tcgcccagcg cctggcgcgc aagctctgca cccactgcaa
     1861 gcagcgcgcg atcgtgccgc ccgaggcgct cgccgacgcc gacatccgcg tcggcggcga
     1921 g
//
LOCUS       DATABV010000083         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_909687, whole genome shotgun sequence.
ACCESSION   DATABV010000083 DATABV010000000
VERSION     DATABV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909687"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VHF88_03410"
     CDS             <1..859
                     /locus_tag="VHF88_03410"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX2160848.1"
                     /translation="DETAAAGEVTPARIGDGAPAARASTAVPAAAPRSRLAPLRRALH
                     VPAALHRELARRGAQTALDAPAGERREVAFADADLAEVRRIGHALPDRATVNDVVLAA
                     VAGALRSWLDGLGAHPDALRAKVPVSLHHPGDDAGNRDSFICVDLPVEQSDPVERLRA
                     VAEETRERKRDRDAETLDSFFRDLSHVSSSLERFAEHWALSPRVFTLNVSNVPGPQGP
                     LAVLGAPVASLHSLAEIAHRHALRVAVVSAGGRIGFGFCADADAVGSPQPIADGVEAE
                     LRALSTALR"
     gene            complement(921..1736)
                     /locus_tag="VHF88_03415"
     CDS             complement(921..1736)
                     /locus_tag="VHF88_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2160849.1"
                     /translation="MATVGDQRGTVDGIATRWLQADTDGTHAPALYVHGVPTAAWLWR
                     DFLARSGGYAPDLPGFGASDKLASFDYSIPGYARWLRAFADQRGLDRFSLVVHDWGGG
                     IGLALAQAIPERIERLAIVSSVPLLPGYRWHRVARNWRRPLIGELAMGFTFKVALGRV
                     LDGSNREPLPGGFVDAVWEHFDHGTQRAILKLYRSAPSDVLAAAGANLGALRCPAIVV
                     YGGRDPYLPAEFAQRYADALGGPATARVIEDAGHWTWLDEPSLIDEIAAFLSA"
     gene            1756..2478
                     /gene="nth"
                     /locus_tag="VHF88_03420"
     CDS             1756..2478
                     /gene="nth"
                     /locus_tag="VHF88_03420"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX2160850.1"
                     /translation="MSRAADWKPPPPRRLRAILERLRRAYGRPATRIHGAPIDELVLT
                     VLSQNTNDRNRDVAYERLRERFPSWEAVRDAPVEEIEEAIRPGGLAVTKAPRIQRILE
                     AISERPAPPGMDGTEAGSMDLAWMAEAPLDEARDYLCALPGVGRKTAACVLLFSYGRH
                     DVPVDTHVHRVGTRLGLFRESASLDDCHDEMLRLSAGSDPLEAHVALIRHGRRICVAR
                     RPRCEECPLLRICPYGRSTVRE"
     gene            2543..>2905
                     /locus_tag="VHF88_03425"
     CDS             2543..>2905
                     /locus_tag="VHF88_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX2160851.1"
                     /translation="MGKTIGIDLGTTNSCMAVLDGGEPTVVENAEGARTTPSVVAFTD
                     SGERLVGAPAKRQAVTNPENTVFSIKRFMGRKEAEVKEEETIVPYEVVRGPNGDARVK
                     AAGKEYSPPEISAMILQKL"
BASE COUNT          416 a         1050 c         1055 g          384 t
ORIGIN      
        1 cgacgagacc gcggccgcgg gcgaggtgac cccggcaagg atcggcgacg gtgcgcccgc
       61 cgctcgcgca tcgaccgccg tcccggccgc cgcgccgcgc tcgcgcctcg ccccgctgcg
      121 ccgcgcactc cacgtcccgg ccgcgctaca ccgcgagctc gcccgccgcg gcgcgcagac
      181 ggcgctcgac gcgcctgccg gcgagcgccg cgaagtggcg ttcgccgatg ccgacctggc
      241 cgaggtcagg cggatcggtc acgcgctgcc agatcgggcg accgtcaacg acgtcgtcct
      301 agcggctgtc gcaggcgcgt tgcgctcttg gctagacggc ctcggagcgc acccggatgc
      361 cttgcgggcc aaggtgcccg tcagcctcca ccatccgggc gacgacgccg gcaaccgcga
      421 ctcgttcatc tgcgtcgacc tgccggtcga gcagagcgat ccggtcgagc ggctgcgggc
      481 ggtcgccgag gagacgcgcg agcgcaagcg cgaccgtgac gccgagacgc tcgactcgtt
      541 cttccgcgac ctctctcacg tgtcgagctc gctggagcgc ttcgccgagc actgggcgct
      601 gagcccgcgc gtgttcacgc tgaacgtgtc gaacgtgccc ggtccgcagg gcccgctcgc
      661 ggtcctcggc gccccggtcg cctctctgca ctcgctggcg gagatcgcgc accgccatgc
      721 gctccgggtg gcggtcgtct cggctggcgg acggatcggg ttcgggttct gcgccgacgc
      781 cgatgcagtc ggctcgccgc agccgattgc cgacggcgtc gaggcggagc ttcgggcgct
      841 ctcgaccgcc cttcgctgag ggctgccgcc cgggcgaacg atcgtcgggc ctctaggccc
      901 aaaaagactt cgcgcgtcgg ctacgcggac aggaaagcgg cgatctcgtc gatgagcgac
      961 ggctcgtcga gccacgtcca gtggccggcg tcctcgatta cgcgggcggt cgcggggccg
     1021 ccgagcgcgt cggcgtagcg ctgggcgaac tccgccggca ggtacgggtc gcggccgccg
     1081 tagacgacga tcgcggggca gcggagcgcg ccgaggttgg cgccggcggc ggcgaggacg
     1141 tcggagggcg ccgatcggta gagcttgagg atcgcccgct gggtgccgtg gtcgaagtgc
     1201 tcccagacgg catcgacgaa gccgccgggc aggggctcgc ggttcgaacc gtcgaggacg
     1261 cggcccaggg cgaccttgaa cgtgaacccc atcgccagct cgccgatcag cgggcggcgc
     1321 cagttgcgcg ccacccggtg ccagcgatag ccgggcaaca gcggtacgga ggacacgatc
     1381 gccagccgct cgatccgctc ggggatcgcc tgggcgagcg cgagtccgat cccgccgccc
     1441 cagtcgtgta ccaccagcga aaagcggtca aggcctcgct ggtcggcgaa cgcccgcagc
     1501 cagcgggcgt agccggggat cgagtagtcg aagctggcca gcttgtcaga ggcgccgaag
     1561 cccgggaggt cgggggcgta gccgccgctg cgcgcgagga agtcgcgcca tagccaggcc
     1621 gcggtcggta cgccgtggac gtagagcgcc ggcgcgtgcg tgccgtcggt gtccgcctgc
     1681 agccaccgcg tcgcgatgcc gtcgacggtc ccccgctggt cgccgaccgt cgccatggcg
     1741 tgtttcatat cagccatgtc gcgcgccgcc gactggaagc cgccgccccc acgacgcctg
     1801 cgggcgatcc tggagcggct gcgccgtgcg tacggacggc cggcgacgcg catccacggg
     1861 gcgccgatcg acgagctggt cctcaccgtc ctctcgcaga acaccaacga ccgcaaccgc
     1921 gacgtcgcct acgagcggct gcgcgagcgc ttcccgtcct gggaggcggt gcgcgacgcg
     1981 ccggtcgagg agatcgagga ggcgatccgg ccgggcggcc tggcggtgac caaggcgcca
     2041 cggatccagc ggatcctcga ggcgatctcc gagcggccgg ccccgcccgg catggacggc
     2101 acggaggccg ggtcgatgga cctcgcgtgg atggccgagg cgccgctgga cgaggcgcgc
     2161 gactacctct gcgccctgcc cggcgtcggc cgcaagaccg ccgcctgcgt gctgctcttc
     2221 tcctacggcc gccacgacgt gccggtcgac acccacgtcc accgcgtcgg cacccgcctc
     2281 ggcctgtttc gcgaaagcgc ctcgctcgac gactgccacg acgagatgct gcgcctgagc
     2341 gcgggttcgg acccgctcga ggcccatgtc gcgctgatcc gtcacggacg tcgcatctgc
     2401 gtcgcccgcc gcccgcgctg cgaggagtgc ccgctgcttc gcatctgccc ttacggccgg
     2461 tcgacggtcc gcgaatgatc tgccatactg gcgcttagat tttcttcctc gagaactgaa
     2521 agccagtaga aggagcgacg cgatgggcaa gaccatcgga atcgatctag gcacgacgaa
     2581 ctcgtgcatg gccgtgctcg acggcggtga gcccacggtc gtcgagaacg ccgagggcgc
     2641 gcggacgacg ccgtccgtgg tcgccttcac cgacagcggc gagcggctcg tcggcgcgcc
     2701 cgccaagcgc caggcggtga ccaaccccga gaacacggtc ttctcgatca agcgcttcat
     2761 gggtcgcaag gaggcggagg tcaaggagga ggagacgatc gtcccgtacg aggtcgtccg
     2821 cggcccgaac ggcgacgcgc gcgtcaaggc ggccggcaag gagtactcgc cgccggagat
     2881 cagcgcgatg atcctccaga agctg
//
LOCUS       DATABV010000084         9837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1392114, whole genome shotgun sequence.
ACCESSION   DATABV010000084 DATABV010000000
VERSION     DATABV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392114"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHF88_03430"
     CDS             <1..199
                     /locus_tag="VHF88_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2160852.1"
                     /translation="KYSSMAKLFASDVAMEVTVEAVQVLGGYGYVNEYPVERMMRDAK
                     ITQIYEGTNEIQRMVVSRSMR"
     gene            239..327
                     /locus_tag="VHF88_03435"
     tRNA            239..327
                     /locus_tag="VHF88_03435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:273..275,aa:Ser,seq:cga)
     gene            complement(339..1412)
                     /locus_tag="VHF88_03440"
     CDS             complement(339..1412)
                     /locus_tag="VHF88_03440"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HEX2160853.1"
                     /translation="MPAERARRLRGVLRRYSGCLGALPQRGRRALELRAGLGDGGPAS
                     QRTIARRIGMSPNAVLRLQRSSLRRLIDSGESGACDGAAAAAAAIAGTATGGDGLAEP
                     PDDGAGTSPSASAGRGADDGEAPAAGRVLADTHLGGPGIDLGDGEEAPVETLLFFLLT
                     LLAVLGPLVLIALLLQRRAAAVGDKRPLLFLDIDGVIALSVFSNRLPPGEMRNTEFGV
                     IHIPDRAGDLLRTLASRFDLVWATGWEHAANSDLGGPLGLREELPVLTFGKKARSGPS
                     DWKIKRVSAHARRRPAAWVDDSFAARHERWAAHRSEPTLLMPVDANTGLTEDHVERLL
                     RWAGRLEASQAAEANGLRRTRAN"
     gene            2171..2731
                     /locus_tag="VHF88_03445"
     CDS             2171..2731
                     /locus_tag="VHF88_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160854.1"
                     /translation="MPQLTSSWARTAACCALACIAGIGTSVGVASGKDGADDTTGTTT
                     GETTNGTTTDGTTTGTTTDDSTTDRTTTDRTTTDGSTTDRTTTAPYVGPPRAPRILEL
                     EADWRANGKIRLRTELARRGAAIGSVRFRYRGKGFKAKKVGRHEWAKSVRPRGGDSRG
                     DVITFRVRACAAKRCNVRTGSDEAGG"
     gene            complement(2728..4704)
                     /locus_tag="VHF88_03450"
     CDS             complement(2728..4704)
                     /locus_tag="VHF88_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2160855.1"
                     /translation="MAVAIASALDKDMAGSPLLRGVSFKLERRDRMTLSGRNGSGKTT
                     LLRMLAGQASIDGGELSIEKGARVTLHDQRPPRERDVTVREYVVSGCGDIAALEDELT
                     RLGAEMAERPDDEALLQRYSRAQDRFEHAGGWDWRRRALEPLRGLGFRDEQLDRRLDT
                     FSGGELTRASLARALAGAPDLLLLDEPTNHLDIDSLEWLEKYLQELDAAVVLVAHDRW
                     FLEAVGTSVLELEAGKARFFSGPWHAWRREKAAREIALGRAIDKQQAEIARMERFVER
                     FRAKATKARQAQSRVKKLEKMERIERDPRESAGLSFQFAGAERSGRVVFELDGGRIDV
                     PAREGEDDGDRRTLIDPAELWLERGEHVALVGPNGSGKTTLIETLAGRRALPTGKLRT
                     GHNVELSYLSQHAEWLGESGSVLDAAQRETKLSPNKARALLGRFLFSGDDALKQLAGL
                     SGGERQRLSLAILVNSGANLLVLDEPTNHLDVESREALEDALRGYDGTVLLVSHDRAL
                     LDAVGTRTIAIEDGTLRSYEGGWADYARIREQRRGMAAEEKTVTRAEEKASARSAKAS
                     AGSGKANGTPSASGNGSGKPLSKNMRRKIGALEREVERAEQALKAIEDELADPSAWST
                     PERSADATARHEAAKQAVRDAYERWEQATAVEAG"
     gene            4818..6893
                     /locus_tag="VHF88_03455"
     CDS             4818..6893
                     /locus_tag="VHF88_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2160856.1"
                     /translation="MRSFARRLRGAAYDGLRLVLGVPWITGRLGGVIDDGRRQTRLGA
                     FFDRRPLLLRSLAIVALIWGAVYLTWRVGWSGEGANPVVFAMLLVTELYGVYALAMLA
                     WFSWSRPAAARPPVTTQHAVDVYVCTYDEPVEVVLATLAGCRALTYPHTTYLLDDGRR
                     PEMEELARLSGARYLTRPDNSHAKAGNINAALPRTEGELVFVLDADHVPMPDALDTLV
                     GYFDDDRMAVVQSPHDFSNHDSVQHYMVGRHEQSLFYRVICPGKDRHGAAFWCGSAAL
                     IRRRALLDIGGVATETIAEDFHTTIRLQRHGWRSRYHDEVLVQGLAPHDLDGYLLQRD
                     RWARGNLAVFTLPESPLRARELAPAQRLSYLASLFAYLAPPMRLLLLTTLAVVLWTGA
                     LPMKLSVAALAVLWLPSVVLNLAAGSALARGYMRIAETTHFELLTMEIYTRALRCAVI
                     PGKTAFKVTPKQGVDEGGWRAVMKLRLVALCAIVLAAGIAVRGVDIFGPALIPSLPGV
                     AAVIVPLLALIELRRVLRTLVLAGARRQRRLVYRFEGVDARAHTFAGARPVLGRVVDA
                     SASGLGLVMDRPLKVGSRPLVRLRLPDATGAEHDVPVRVEVRSSRSAGESTVIGASIV
                     SIDPESRLRLMEWCYVVCSHERLRGHRPAAPADTLPAPIVFPVEDTDTAPVHVFAPDA
                     EGASAAATA"
     gene            7232..8170
                     /locus_tag="VHF88_03460"
     CDS             7232..8170
                     /locus_tag="VHF88_03460"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX2160857.1"
                     /translation="MQRVVGPLGALRIRGTGDIAVAALATAQNGVVSRAQLEAVGLGR
                     GSIARRVANGRLHRLHRGVYAVGHQALPPHARETAALLACGATAVLSHRSAAAAWGLL
                     PKSPGAVDVTVPSRDCRPRAGIRAHTTSDLPSDDVSRLDRLPLTTVARTLVELAGEVE
                     TRELERALDEAAARRLATHTEIRSVLRRAKGRSGTRALRDLLDDLDGPRLTRSEAERR
                     LLALLRVARLPLPQTNVRVGCHEVDALWQSQRLVVEVDGYAFHSSRAAFERDRLRDSD
                     LQARGLRVVRITWRQLSDEPEATVARIARVLGAAGP"
     gene            complement(8214..9110)
                     /locus_tag="VHF88_03465"
     CDS             complement(8214..9110)
                     /locus_tag="VHF88_03465"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX2160858.1"
                     /translation="MDSQIEQDAPASDFDRATAVAALGDGRFAGEVEDGWGAPPGPNG
                     GYLAAILTRALEHELAPGGERQLRTLATHYLRPSQPGPIEVAIETIRAGRRFSTGRAT
                     AFQNGKEVMTALGAFSTAGLPAAATWSPALPDVAPAPPRDAAHVPSEDYRRESDGWLA
                     TTPEMPSIVSRVKIAPRVGDKPFSGRELAPGEAAETGGWIQLPTPRRIDAAYVVLLSD
                     VWWPPSLEPLTSPAIAPTIDLTVHIRADLPPDGYPDQPVLGRFRTAAAVNGTMEEDGE
                     LYLADGTLIAQSRQLALLAPFG"
     gene            9177..9749
                     /locus_tag="VHF88_03470"
     CDS             9177..9749
                     /locus_tag="VHF88_03470"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX2160859.1"
                     /translation="MRDTLRAPLRAGAAAAFTVGMLLFLLNPPELSGAEDLITLVMGA
                     VALVLFTAWGTVVLMGDGPSEPEIERIVQRSETLARLPVPETAPSDFDDLVTEALDEL
                     PDEFQEVLRDIPVVVSRQGREHGAYGHYYGDTVARDNYPDRIIIYQDTLERDFGHDRD
                     LLRSQVTRTVRHEIAHHLGWDERGVRDLGL"
BASE COUNT         1301 a         3621 c         3548 g         1367 t
ORIGIN      
        1 caagtactcc tcgatggcga agctgttcgc ctccgacgtc gcgatggagg tcacggtcga
       61 ggcggtccag gtgctcggcg gctacggcta cgtgaacgag tacccggtcg agcgcatgat
      121 gcgcgacgcc aagatcaccc aaatctacga gggcaccaac gagatccagc ggatggtcgt
      181 ctcgcgctcg atgcgctagc cgctccgcta gccttgcgac cgtcccggag cggacctcgg
      241 agaggtgccg gagtggttga acggggcggt ctcgaaaacc gttaagggcg gcaacgtcct
      301 tcgagggttc gaatccctcc ctctccgctg cgccggtctc agttcgcgcg tgtccggcgc
      361 aggccgttcg cctcggcggc ctgcgacgcc tcgaggcggc ccgcccagcg cagcagccgc
      421 tcgacgtggt cctcggtcag gcccgtgttc gcgtcgacgg gcatcagcag cgtcggctcc
      481 gagcggtggg cggcccagcg ctcgtggcgg gcggcgaacg agtcgtcgac ccacgccgcc
      541 gggcgtcttc tggcgtgggc gctgacgcgc ttgatcttcc agtccgacgg acccgaccgc
      601 gccttcttgc caaaggtcag gaccggcagc tcctcgcgaa gcccgagcgg cccgcccagg
      661 tcgctgttcg cggcgtgctc ccagccggtg gcccagacga ggtcgaagcg gctcgcgagc
      721 gtgcggagca ggtcaccggc acggtcgggg atgtggatca caccgaactc cgtgttgcgc
      781 atctcgcccg gcggaaggcg gttggagaac acgctcagag cgatcacgcc gtcgatgtcg
      841 aggaagagca gcggacgctt gtcgccgacc gccgcggcgc ggcgctgcag cagcagcgcg
      901 atcaggacga gcggcccgag caccgccagc aacgtgagca ggaagaacag cagcgtctcg
      961 accggggcct cctcgccgtc gccgaggtct atccccgggc cgccgaggtg cgtgtcggcc
     1021 aggacccggc ccgccgcggg cgcctcgccg tcatcagcgc cgcgccctgc ggaggccgag
     1081 gggctcgtgc cggcgccgtc gtcgggcggc tcggcgagac cgtctccgcc agtagccgta
     1141 ccggcgatcg cggctgcggc cgccgctgcg ccgtcgcatg cgccgctctc tccgctgtcg
     1201 atcaagcggc gcagcgagga gcgctggaga cgcagcacgg cgttgggcga catgccgatc
     1261 cggcgggcga tcgtccgctg cgacgcggga ccaccgtcac cgaggccggc acgcagctcg
     1321 agcgcccgcc gaccgcgctg tggcagtgct ccgaggcagc cgctgtagcg ccggagaacg
     1381 ccgcgcaggc gacgcgcacg ctccgcgggc aacggtgcgc cgagcaccgc ggacgatcga
     1441 tccggcccgc cgaacgaccg cgacgtgggg tcggggccga gcaggctccg cagtgacggc
     1501 gggcgtgatg ccgacgtcgg cacgctgccc ggatcggcag caccggctgg cggcgtaacc
     1561 gacgtggcgg gggtgcgcgg ctgcgacgac gcgggcgcgc cgagacccgc cggtgactgg
     1621 atcgatcgcg cgggcgcgtc gaccggcggc gccggcggcc tgacgggcag cgatggcgac
     1681 ccgacgggga ccgatggcgg tatcagaggc gccgagggcg gctcgatggg acgcgaaggc
     1741 ggcgcgacgg gcgccggcgc gacaggggcg ggcggaagct ccacatccgg gaccgggacc
     1801 ggggccgggg ctggaactgg agccggaggt acctcgacgc gcggcggcgg cacgtcgacc
     1861 cggactccgg gtgcgacgac ggtcaccgat ggcgtgtgga cgccgagcgc ctgcgcccgc
     1921 tcgtgagcgc ccgcgacacc ggcggcgacc gccagcgcga tcgtggtgcc tatggcgagg
     1981 cggcgtccgg cccctcgatg actgcgggtc ccttcctcca cgcacttctc cggtcgagat
     2041 gcgcaccttc cgcagcggaa tggctacccg aacccgtgca tctgaatcgt cgctttcgcg
     2101 gtcgcgtttg cgaacgcgcg ggttcgttcc taccctggcc gtccacgact catcgacccc
     2161 aggagaagga atgcctcagc tgacctccag ttgggcgcgc acagccgcgt gttgcgccct
     2221 ggcctgcatc gccggcatcg gcaccagcgt cggcgtcgcg tccggcaagg acggagccga
     2281 cgacacgacc ggcacgacca ccggcgagac cacgaacgga accacgacgg acggcacgac
     2341 caccggcacg acgaccgacg actcgacgac cgaccggacg acgaccgacc ggacgacgac
     2401 cgacggcagc acgaccgatc ggaccacgac ggcgccgtac gtcggcccgc cgcgcgcacc
     2461 gcggatcctc gagctcgagg ccgactggcg ggcgaacggc aagatccgcc tgcgcaccga
     2521 gctcgcgcgg cgcggggcgg cgatcggcag cgtgcggttc cgttaccgcg gcaagggctt
     2581 caaggccaag aaggtcggcc gccacgagtg ggcgaagtcg gtcaggccgc gtggcggcga
     2641 cagccgcggc gacgtgatca ccttccgcgt ccgggcgtgt gccgccaagc gctgcaacgt
     2701 gcggaccggc agcgacgagg cgggcggcta gcccgcctcg acggcggtgg cctgctccca
     2761 gcgctcgtag gcgtcgcgga ccgcctgctt ggcggcctcg tggcgggcgg tcgcgtccgc
     2821 tgagcgctcg ggagtggacc acgccgacgg gtcggcgagc tcgtcctcga tcgccttgag
     2881 cgcctgctcg gcgcgctcga cctcgcgttc gagcgcgccg atcttgcggc gcatgttctt
     2941 cgagagcggc ttgcccgagc cgttgccgct cgcgctcggc gtgccgttcg ccttcccgga
     3001 cccggcgctc gccttcgccg accgagcgct cgccttctct tccgcgcgcg tgactgtctt
     3061 ctcctctgcc gccataccgc gccgctgctc gcggatccgc gcgtagtcgg cccagccgcc
     3121 ctcgtagctg cgcagcgtgc cgtcctcgat cgcgatcgtc cgagtgccga cggcgtcgag
     3181 cagcgcgcgg tcgtgggaga ccagcagcac cgtgccgtcg tagccgcgca gcgcgtcctc
     3241 gagcgcctcg cggctctcga cgtcgaggtg gttggtcggc tcgtcgagga ccagcaggtt
     3301 ggcgccggag ttgacgagga tcgccagcga cagccgctgg cgctcgccgc ccgagagccc
     3361 ggcgagctgc ttgagcgcat cgtcgccgga gaagaggaag cggccgagca gcgcgcgcgc
     3421 cttgttcggc gacagcttcg tctcgcgctg ggcggcgtcg agcacgctgc cgctctcgcc
     3481 aagccactca gcgtgctggg agaggtagct cagctcgacg ttgtggccgg tgcgcagctt
     3541 gccggtgggc agcgcgcgcc gcccggcgag cgtttcgatc aacgtcgtct tgccggagcc
     3601 gttcggtccg accagcgcga cgtgctcacc gcgctcgagc cacagctcgg cggggtcgat
     3661 cagggtgcga cggtcgccgt cgtcctcgcc ctcgcgagcc gggacgtcga tccgcccgcc
     3721 gtcgagctcg aagacgacgc ggcctgagcg ctcggcgccg gcgaactgga acgacagccc
     3781 ggcgctctcg cgcgggtcgc gctcgatccg ctccatcttc tccagcttct tgacgcgcga
     3841 ctgcgcctgg cgcgcctttg tcgccttggc gcggaagcgc tcgacgaacc gctccatccg
     3901 cgcgatctcg gcctgctgct tgtcgatcgc ccgcccgagc gcgatctcgc gggccgcctt
     3961 ctcgcgccgc caggcgtgcc acggccctga gaagaagcgc gccttgccgg cctcgagctc
     4021 gagcaccgac gtgccgaccg cctcgaggaa ccagcggtcg tgggcgacca ggacgacagc
     4081 cgcgtcgagc tcctggaggt acttctcgag ccactcgagc gagtcgatgt cgaggtggtt
     4141 ggtcggctcg tcgagcagca gcaggtcggg cgcgccggcg agcgcgcgcg ccagcgaggc
     4201 gcgggtgagc tcgccaccgg agaaggtgtc gaggcggcga tcgagctgct cgtcgcgaaa
     4261 gccgagcccg cgcagcggct ccagcgcccg ccgccgccag tcccagccgc cagcgtgctc
     4321 gaagcggtcc tgcgcgcgcg agtagcgctg cagcagcgcc tcgtcgtcgg gacgctcggc
     4381 catctcggcg ccgagccgcg tcagctcgtc ctcgagcgcc gcgatatcgc cgcagcccga
     4441 gacgacgtat tcgcggaccg tcacgtcgcg ctcgcgcggc ggtcgctggt cgtgcagcgt
     4501 gacgcgcgcg cccttctcga tcgacagctc gccgccgtcg atcgacgcct ggccggcgag
     4561 catccgcagc agcgtcgtct tgccgctgcc gttgcgcccc gagagcgtca tccggtcgcg
     4621 ccgctcgagc ttgaacgaga cgccgcgcag cagcggcgag ccggccatgt ccttgtccag
     4681 cgctgatgcg atcgctacgg ccatcgagcc gacgatggta ggcgacccgc cgatcggcgt
     4741 cggccgggcg ccgccgcgtg aagggggcga gcgcggtcgc gcctaaaggc agactgccgg
     4801 ctgccgatct atccgccatg cgctcgttcg cccgccgcct gcgcggcgcc gcctatgacg
     4861 gcctccggct ggtcctcggc gtgccgtgga tcaccggccg cctcggcggc gtgatcgacg
     4921 acgggcggcg ccagaccagg ctcggcgcgt tcttcgaccg tcggccgctg ctgctgcgct
     4981 cgctggcgat cgtcgcgttg atctgggggg cggtctatct gacctggcgc gttggctgga
     5041 gcggcgaggg cgccaacccc gtcgtcttcg cgatgctgct cgtgaccgag ctctacgggg
     5101 tctacgcgct cgcgatgctc gcctggttct cgtggtcgcg cccggcagcg gcgcgaccgc
     5161 cggtcacgac tcagcacgcc gtcgacgtct acgtctgcac ctacgacgag ccggtcgagg
     5221 tcgtgctggc cacgctcgcc ggctgccgcg cgctgaccta cccgcacacc acctacctgc
     5281 tcgacgacgg ccggcggccg gagatggagg agctcgcgcg gctgagcggc gcgcgctacc
     5341 tcacgcggcc cgacaactcg cacgccaagg ccggcaacat caacgcggcg ttgccgcgca
     5401 cggagggcga gctcgtcttc gtgctcgatg ccgaccacgt cccgatgccc gacgcgctcg
     5461 acacgctggt cggctacttc gacgacgacc ggatggcggt cgtccagagc ccgcacgact
     5521 tctccaatca cgactcggtc cagcactaca tggtcggccg ccacgagcag tcgctcttct
     5581 accgcgtgat ctgccccggc aaggaccgcc acggcgccgc cttctggtgc ggctcggcgg
     5641 ccctgattcg gcgccgtgcg ctgctcgaca tcggcggcgt cgcgaccgag acgatcgccg
     5701 aggacttcca caccacgatc cgcctgcagc gccatggctg gcgcagccgc taccacgacg
     5761 aggtgctcgt gcagggtctc gcgccgcacg acctcgacgg ctacctgctg cagcgcgacc
     5821 gctgggcgcg cggcaacctc gccgtcttca cgctgcccga gtcgccgctg cgggcacgcg
     5881 agctcgcgcc ggcgcagcgg ctctcgtacc tcgcgtcgct gttcgcctac ctggccccgc
     5941 cgatgcgact gctgctgctg accacgctgg ccgtcgtgct ctggaccgga gcgctgccga
     6001 tgaagctgag cgtcgccgcg ctcgccgtcc tgtggctgcc gtcagtggtg ctcaacctgg
     6061 cggcgggctc cgcgctcgcg cgcggctaca tgcggatcgc cgagacgacc cacttcgagc
     6121 tgctgacgat ggagatctac acgcgcgcgc tccgttgcgc cgtgatcccg ggcaagaccg
     6181 ccttcaaggt gacgccgaag cagggggtgg acgagggcgg ctggcgcgcc gtgatgaagc
     6241 tgcgcctggt cgcgctgtgc gcgatcgtgc tggccgcggg aatcgccgtg cgcggcgtcg
     6301 acatcttcgg cccggcgctg atcccgtcgc ttccgggcgt tgcggcggtg atcgtgccgc
     6361 tgctcgcact gatcgagctg cgccgcgtgt tgcgcacgct ggtcctcgcg ggcgcgcgcc
     6421 gccagcggcg actggtctat cgcttcgagg gcgtcgacgc gcgggcccac accttcgccg
     6481 gcgcgcggcc ggtgctcggc cgcgtcgtcg acgccagcgc ctccggcctc ggcctcgtca
     6541 tggacaggcc gctgaaggtc ggctcgcgcc cgctcgtccg cctgcgcctg cccgacgcca
     6601 ccggcgccga gcacgacgtg ccggttcgcg tcgaggtccg ctcctcgcgc agcgccggcg
     6661 aaagcacggt gatcggggca agcatcgtct cgatcgaccc cgagtcccgc ctccgcttga
     6721 tggagtggtg ctacgtcgtc tgcagccacg agcggctgcg cggccaccgt cccgccgcgc
     6781 ccgccgacac gctcccggcc ccgatcgtct tccccgtcga agacaccgac accgcgcccg
     6841 tccacgtctt cgcgccggac gccgagggcg cctcggcagc cgcgaccgcg tagagcgggc
     6901 cccgtcggcg taggccgcga cagccgatcc gtcgtcgggt aacggactcg gcacgaacgt
     6961 gacagccgcg taacggccgc tagcttcgtc gcggtccggc cggcaagctg cggccatgcc
     7021 cgccgtctcc gctgctgcag aagtcccgtc gctcgccgcg gccgctccgc cgcaggccgt
     7081 agtgcaccgt gacggtgcaa aagtcccgtc cgcagccctc ctggcccctg cgacggaaga
     7141 agcgcacgcc gaggatgcgg tacggcctcg gcccggcgcg agggcgagcg cggcgggcga
     7201 agtgcacggt cgtgaaaggg gaggagcgcc ggtgcagcgg gtcgtcgggc cgcttggcgc
     7261 acttcggatt cgagggactg gcgacatcgc ggtcgccgcc ctcgcgaccg cccagaacgg
     7321 cgtcgtctcg cgcgcgcagc tcgaggcggt cggtctcggt cgcggctcga tcgcgcgacg
     7381 ggtcgccaac ggtcgtctgc accgcctcca tcgcggcgtc tatgccgtgg gccaccaggc
     7441 gctgccgccg cacgcgcggg agaccgccgc cctgctggct tgcggagcga ccgccgtgct
     7501 cagtcaccgc agcgccgccg ccgcgtgggg cctgctgccg aagtcgcccg gggcggtaga
     7561 cgtgaccgtg ccaagtcggg attgccgccc acgcgcgggc atccgcgctc acactacgag
     7621 cgatctcccg tccgacgacg tgagtcggct cgacaggctg ccgctgacaa ccgtggcgcg
     7681 gacgctggtc gaactcgcgg gtgaggtgga gacgcgcgag ctggagcgcg cgctcgacga
     7741 ggccgcggcg cgcaggctcg ctacccacac ggagatccgc agcgttctgc gacgggcgaa
     7801 ggggcgttcg gggacacgcg ccctacgcga tctgctcgac gacctcgacg ggccgcgact
     7861 gacccgctcc gaggcggagc gtcggctact ggcgctgctg cgcgtcgccc ggcttccgct
     7921 cccgcagacc aacgtccggg tcgggtgcca cgaggtcgac gcgctctggc agtcccagcg
     7981 gctcgtggtc gaggtggacg gctatgcgtt ccattcgagc cgcgcggcgt tcgagcgcga
     8041 tcggctgcgc gacagcgatc tccaggcgcg tggcctgcgg gtggttcgca tcacctggcg
     8101 ccagctcagc gacgagccgg aggcgacggt cgcccggatc gctcgcgtac tgggagcggc
     8161 aggcccctag ccaaacgcga gccccgcccg tcttggcgtg cgccggccgc gcctcagccg
     8221 aacggcgcca gcagggcgag ctggcgcgac tgggcgatca gggtgccgtc ggcgaggtag
     8281 agctcgccgt cctcctccat cgtgccgttg accgcggcgg cggtgcggaa gcggccgagg
     8341 acgggttggt ccgggtagcc gtcgggcgga aggtcagcgc ggatgtggac ggtgaggtcg
     8401 atcgtcggtg cgatcgccgg gctggtcaga ggctccagcg acggcggcca ccagacgtcg
     8461 gacagcagga cgacgtacgc cgcgtcgatc cggcgcgggg tcggcagctg gatccagccg
     8521 cccgtctcgg ccgcctcgcc cggcgccagc tcacgacccg agaacggctt gtcgccgacg
     8581 cgcggcgcga tcttcacgcg cgagacgatc gacggcatct ccggcgtcgt cgccagccag
     8641 ccgtcgctct cccgccggta gtcctccgag ggcacatggg cggcgtcgcg cggcggcgcc
     8701 ggcgccacgt cgggcagcgc cggggaccac gtcgcggcgg ccggcagccc cgccgtcgag
     8761 aacgcgccga gcgccgtcat cacttccttg ccgttctgga acgccgtcgc acggccggtc
     8821 gagaaacgcc tgccagcgcg aatcgtctcg atcgcgacct cgatcgggcc cggctgcgac
     8881 ggcctcaggt agtgcgtcgc gagcgtgcgc agctggcgct ccccgcccgg tgcgagctcg
     8941 tgctccagcg cacgcgtcag gatcgctgcg aggtagccgc cgttcggacc gggcggcgcg
     9001 ccccagccgt cctcgacctc gccggcgaag cggccgtcgc cgagcgcagc gaccgccgtt
     9061 gcgcggtcga agtcgctcgc cggcgcgtcc tgctcgatct gcgagtccac gggccgggca
     9121 cgctaacagg gctggctggc cagccaggct tgcgccgcct tctacgcttg acgtggatga
     9181 gggacacgct gcgagcacca ctgcgggccg gcgcagctgc cgcgttcacc gttgggatgc
     9241 tgctcttcct gctcaacccg ccggagctct cgggcgctga ggacctgatc acgctcgtga
     9301 tgggggccgt cgcgctcgtc ctcttcacag cctggggcac ggtcgtgctg atgggcgacg
     9361 ggccgagcga gccggagatc gagcggatcg tccagcgcag cgagacgctc gcccgcctgc
     9421 cggtccccga gaccgccccg agcgacttcg acgacctcgt caccgaagcg ctcgacgagc
     9481 tgcccgacga gttccaggag gtcctgcggg acatccccgt cgtggtctca cgccagggcc
     9541 gcgagcacgg cgcctacggc cactactacg gcgacaccgt cgcgcgcgac aactaccccg
     9601 accggatcat catctaccag gacacgctcg agcgcgactt cggccacgac cgcgacctgc
     9661 tgcgctccca ggtcacgcgc accgtccgcc acgagatcgc ccaccacctc ggctgggacg
     9721 agcgcggcgt gcgcgacctg ggcttgtagg cttgccggac cccggtgagg tgtccgagtg
     9781 gcttaaggag cacgcttgga aagcgtgtat gcgggaaacc gtatcgtggg ttcgaat
//
LOCUS       DATABV010000085         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_26321, whole genome shotgun sequence.
ACCESSION   DATABV010000085 DATABV010000000
VERSION     DATABV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26321"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VHF88_03475"
     CDS             <1..722
                     /locus_tag="VHF88_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX2160860.1"
                     /translation="FGEDVALYRAISQETPERMAARVAQYRAEGYTKFQLKVGGDPDE
                     DIERIRAAAGELRPGDMLVADANTGWLMHQAARVVRAVRDVDVHIEQPCLGYEECLTI
                     RRRTDHPFVLDESIDGLDALLRGHADGAMDVINLKISKVGGLTKARQIRDLCVALGIA
                     MTIEDSWGGDITTAAIAHLAHSTPEELRFSATDFNSYVTVSTAEGAPRRVQGRMAAST
                     AAGLGIEPRLDVLGEAVFEVG"
     gene            892..2142
                     /locus_tag="VHF88_03480"
     CDS             892..2142
                     /locus_tag="VHF88_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2160861.1"
                     /translation="MSRGSLTGSDEDTALAAKWPSIALLAVCEVLAMALWFSASAVVP
                     ALRLEYPLDDLRVSLITSSVSAGFVAGTLASALLGLADRFDPRRLLAASATVAAGANA
                     AILLVEPTSLALPALRFVVGACAAGIYPVGMKMAASWARGDMGLLVGLLVGALTLGSA
                     SPHLIAALGGGLDWRVTLAASSAFALLAALLIHAVQLGPNRGGPVAFKARYVLLAWTN
                     RPLRLANLGYFGHMWELYAVWGWIGVFLHASFALDPGGEHAALYAKLAAFGTIGIGAL
                     GCLAGGLVADRLGRTTLTMVAMAVSGACCVLAGFLFGAEPWLLAALCLVWGVAMVADS
                     AQFSSSVIELSERELIGTMVTVQTCVGFLITLVTIHLIPTLVDLVTWRYAFAFLAIGP
                     FLGVLAMGRLRRHPESFRLAGGNR"
     gene            2243..2521
                     /locus_tag="VHF88_03485"
     CDS             2243..2521
                     /locus_tag="VHF88_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX2160862.1"
                     /translation="MLRRFIIERELPEVGSFEREQLRAAAEKSNETLRALGPDIQWVE
                     SYVADNKTFCVYLARDEGIIRQHAEKSGFPANKITEVRRIIDPTTAAA"
     gene            complement(2556..>2810)
                     /locus_tag="VHF88_03490"
     CDS             complement(2556..>2810)
                     /locus_tag="VHF88_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2160863.1"
                     /translation="MASPKVLLLDEPSMGLAPRLVDQIFDAVRAVNEQGVTVLLVEQN
                     AFMALEIADLAYVLESGEVVLEGKGLDLLDIEEVKRAYLG"
BASE COUNT          386 a          974 c         1027 g          423 t
ORIGIN      
        1 gcttcggcga ggacgtcgcg ctctaccgcg cgatctcgca ggagacgccg gagcggatgg
       61 cggcgcgcgt ggcccagtac cgggccgagg gctacaccaa gttccagctc aaggtcggcg
      121 gcgacccgga cgaggacatc gagcgcatcc gcgccgcggc cggcgagctt cggccgggcg
      181 acatgctggt ggcggacgcc aacaccggct ggctgatgca ccaggcggcg cgggtagtgc
      241 gcgccgtgcg cgacgtcgac gtccacatcg agcagccctg cctcggctac gaggagtgcc
      301 tgacgatccg ccgccggacc gatcacccct tcgtgctcga cgagagcatc gacgggctcg
      361 acgccctgct ccgcggccac gccgacggcg ccatggacgt catcaacctc aagatcagca
      421 aggtgggcgg gctgacgaaa gcgcggcaga tccgcgacct ctgcgtcgcg ctcgggatcg
      481 ccatgacgat cgaggacagc tggggcggcg acatcaccac ggcggccatc gcccacctcg
      541 cgcactcgac gccggaggag ctccggttct ccgcaaccga cttcaacagc tacgtgacgg
      601 tgagcacggc cgagggcgcg ccgcgccgcg tccaggggcg gatggcggcg tcgacggcgg
      661 cggggctcgg gatcgagccg cggctcgacg tgctggggga ggcggtgttc gaggtcggct
      721 gagcacggtc gcgtagtcca acaaccgaaa cgaagcccgc gtgcgcgaca ccgtgttgag
      781 acgcgacgca cggactcttc gggtcgcggt cggtgcttcg ccgcgggcgg tcctcggggc
      841 gcgaggagag gcagcgggct gcagcgtgga ggcgccgagc gagtgaacga cgtgagccgc
      901 gggtccctga ccggctccga cgaggacacc gcgctcgccg ccaagtggcc gtcgatcgcg
      961 ctgctcgccg tctgcgaggt gctggccatg gcgctctggt tctcggcgtc ggcggtcgtg
     1021 ccggcgcttc ggctggagta cccgctcgac gacctccggg tgtcgctgat caccagcagc
     1081 gtttccgccg gcttcgtcgc cggcacgctg gccagcgcgc tgctcggtct cgccgaccgc
     1141 ttcgatccgc gccggctgct cgccgcctcg gcgacggttg cagccggcgc caacgcggcg
     1201 atccttctgg tcgagccgac ctcgctggcg ctgccggcgc tgcggttcgt cgtcggcgcg
     1261 tgcgcggccg ggatctaccc cgtcggcatg aagatggccg cgagctgggc ccggggcgac
     1321 atgggcctcc tggtcggcct gctcgtcggc gccctgacgc tcggctcggc ctcgccgcac
     1381 ctgatcgcgg cgctcggcgg cgggctcgat tggcgcgtca cgctcgccgc gtcgtcggcg
     1441 ttcgcgctgc tggccgcgct gctgatccac gccgtgcagc tcggtcccaa ccgcggcggc
     1501 ccggtcgcgt tcaaggcgcg ctacgtgctc ctcgcctgga cgaaccggcc gctccggctc
     1561 gccaacctcg gctatttcgg ccacatgtgg gagctctacg ccgtctgggg ctggatcggc
     1621 gtgttcctgc acgcgagctt cgccctggac cccggcgggg agcacgcggc cctctacgcc
     1681 aagctggccg cgttcggcac cattggcatc ggggcgctcg gctgcctcgc cggcgggctc
     1741 gtcgccgacc ggctcgggcg gaccacgctg accatggtgg ccatggccgt gagcggcgcg
     1801 tgctgcgttc tggccggctt cctgttcggc gccgagccct ggctgctggc ggcgctgtgc
     1861 ctggtctggg gcgtggcgat ggtggccgac tccgcgcagt tctcatccag cgtcatcgag
     1921 ctctcggagc gggagctgat cggcacaatg gtgacggtcc agacctgcgt cggctttttg
     1981 atcacgctgg tcacgattca cctgatcccg acgctggtgg acctggtcac gtggcgctac
     2041 gctttcgcgt tcctcgcgat cgggccgttc ctcggcgtgc tcgcgatggg ccggctgcgg
     2101 cggcacccgg agtccttcag gctcgcgggc ggcaaccggt agccgatgga cggacgacgg
     2161 gcggcctcgg gatagcggcc gaggggcgct cggacctaag cggtatcgac acgggcaaca
     2221 gacaccggga ggggagagat ccatgctgcg gcggttcatc atcgagcggg agttgccgga
     2281 ggtcggcagc ttcgagcgcg agcagctcag ggcggcggcg gagaagtcca acgagacgct
     2341 gcgcgcgctc ggtcctgaca tccagtgggt cgagtcctac gtggccgaca acaagacgtt
     2401 ctgcgtctac ttggcgcgcg acgagggaat catccgccag cacgccgaga agagcggctt
     2461 cccggcgaac aagatcaccg aggtcaggag gatcatcgac ccgaccacgg ccgccgcgta
     2521 gaccgcgatc gggctgcgca gacgggcggg gcggctcagc ccagataggc gcgcttgacc
     2581 tcctcgatgt cgaggaggtc gagccccttg ccctccagaa cgacctcgcc cgattccagc
     2641 acgtaggcga ggtccgcgat ctcgagcgcc ataaaggcgt tctgctcgac cagcagcacc
     2701 gtcacgccct gctcgttgac ggcgcgcacg gcgtcgaaga tctgatcgac cagccgcggg
     2761 gcgaggccca tgctcggctc gtcgaggagg agcaccttgg gcgaggccat
//
LOCUS       DATABV010000086        18034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2035421, whole genome shotgun sequence.
ACCESSION   DATABV010000086 DATABV010000000
VERSION     DATABV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18034
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035421"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VHF88_03495"
     CDS             complement(<1..374)
                     /locus_tag="VHF88_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160864.1"
                     /translation="MSLAQRLLALALVVVTVVLLAVLPKLAGNLDAPSVAPIQLREQG
                     GERQRTEPRRNGAARDRSRGRGRAAPPAAGPVPRGTPEPDPPPPTSGGAPATPPASQA
                     PPRVPSPRRRKPPPRPAPAPAPT"
     gene            complement(496..1377)
                     /locus_tag="VHF88_03500"
     CDS             complement(496..1377)
                     /locus_tag="VHF88_03500"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX2160865.1"
                     /translation="MEIKKVGVLGAGLMGHGIAQVVAQAGYDVVLREVDDAKLEKGVG
                     KIEKQLARAVEKGKAEQADADAVRGRIHGTTSYSDLADCELVIEAITEDLALKLEMWK
                     EVDGIVKQGALFATNTSSLSVIDQAASTSRPESFLGLHFFNPAQVMKLVEVVRGITTS
                     DETFAAGVEFTKSLGKLGIPTRDTAGFIVNRLLVPYLLDGIRAYEEGVGSIEEIDAAM
                     KAGAGHPMGPLLLSDFVGLDTLGSICDVLFDEFRERRFARPPTLRKMLAAGWYGRKSG
                     MGFYDYSGDEPVQNPKV"
     gene            1420..2199
                     /locus_tag="VHF88_03505"
     CDS             1420..2199
                     /locus_tag="VHF88_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2160866.1"
                     /translation="MSLIGVEDRGGVRHLVLQRAEKRNAFNRELIEALGHALEDAAAD
                     TSVRVLVVRGDGAMFSSGMDLNDLRELAGDASQLRPFRRAILGWWNLLEEMPKPTIVQ
                     IHGACLGGAFELALAADFRVMAEDAMAGIMEVRVGLLPDVGGCSRLPSIVGLGNAKEL
                     IMTGKVIDGREAHRIGFANRIAPTEELDALTEAFANELLACAPRAVGMAKRVIDASAK
                     PALAATLEQEVQAQEALVASEDFAEGTRAFFEKRQPNFGAK"
     gene            complement(2359..6306)
                     /locus_tag="VHF88_03510"
     CDS             complement(2359..6306)
                     /locus_tag="VHF88_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160867.1"
                     /translation="MDYRLEGLSSRSFEQLIQSIAIAEIGPSVVIFGDGPDGGREAAF
                     KGKIPGLGDGSWDGYLVVQAKFRQVLSDRGRDGQWAAEQLQKEMDRYTASGFTGDRPD
                     YYLYVTNVTLTPVQKQGSKDKVDAILRSAVNSKLLKGYDVWDRDKLCRFLDLHSDLRN
                     AYAAWITAGDVLAKVVDDLAISAPDFDRTLSLFLQKELLADQYANLEQAGRAPEERVP
                     IARVFVDLPLLETEDHFVDEASEPRGLVEQLLDAAHVKLNPGSARSLGPSEGRHPTQV
                     SSKPGRWVLIGGPGQGKTTLGQFVCQLFRASLLAERPPQTISAEARTAMALIDRHCDA
                     SQISGPGVRRFPIRVVLSEFATALEEEDGNLSLLRFITRRVEQRIDRDVPIDAFRQWL
                     NGYPWLIVLDGLDEVPSSSNRDAVLTAINEFWVDIADSETDALVLATTRPQGYNDDFA
                     PSLYLHRTLAPLGQAKALSYGRQLAEARYGADRDRCERVIGRLERAARVETTARLMLS
                     PLQVTIMATLVDQMGQPPEQRWSLFSEYYSVIYNREMERDIPAADILRRQKHNVDRIH
                     RRVALVLQRDSEVARNTESHLSRDRFASIVRGRLEEEAYEGRELQELCDSILAAAAER
                     LVFVVGVEEDRVGFEIRSLQEFMAAEALMDGGDDVVAGRLRSIASASAWRNVFLFAAG
                     RCFADRQHLRDTVYTICAEQNSDLEDAGVSKAAATGASLAIDLSEDGSARDQPRYRRL
                     LLEQALECVDRPASDLQARLARLYDDRSQSAFRDALEAGLRVPVVRSGTWRLLLSLVD
                     GGVHWAEQMLLEAWPDNGGASEAALLGEMDGPLEIGARVSQVLASAALRHGIGYDERY
                     VAMLEMAAKADVPWANLLARGVRRAKHSFGSAIPIAGVEPSSFRLTMTRVTSEDPSLN
                     ASLKEVPNVDVVHPAWRYEAIVGEFVNNPSCTALGDAIDEIMTHELLPYRWGQTGPWP
                     LGACLGAIANGAQAEEIIEAARAGRMGNIDQWRAAEQRWLDGGVTTEDIGHFWEDGLP
                     FDASVAKGGIPLYGCGWNVASPRTAYNVVDAWRHVPAPAGGKQVLARWALAIASLIPD
                     RPDEGDGLLIGLNDFEILVENSGGLRIATEPPTWSEGAQVGVLHYLDGYENPVDLANA
                     LDELGRRSPSARGAFADHTVIIETLTGLVRARPGLMGAWRLLAAAMAQSGYTGWEAPD
                     DVQRRWKLSAQAASITQILARSEPMPSAAEVIDAMSAEPGWIPLLEDALRTVVGDRDA
                     GALVLDFDSHVGVPGNVSEAVLEHLIAETSLRRRAALQEPQRWKELELFDAPD"
     gene            complement(6327..7847)
                     /locus_tag="VHF88_03515"
     CDS             complement(6327..7847)
                     /locus_tag="VHF88_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2160868.1"
                     /translation="MDSHRAGEELASELTRRRLLGGAAALGLGAAVPGLAAGRSEAAA
                     LAPGLIPAANANVVVVVIDSLRTDHVGAYGGRRARTPTLDALATESLLFTHARHDAMP
                     TVPVRKSLLIGRSGFPFRGWRPEGALPAVPGFEGIHRGETTFLDVLGKRGYLTGYVTD
                     NPHLLRPAFARFRGRPDVMTVVEGQIPGWRQRRRPVSPGRLRRHLAPELRGGDRGRVR
                     EYLAYNGASRRERDYLAARVFGGGIDFLEQAAFRAQPFALVVDCFDVHEPWDPPDSYL
                     ARYARIKRGGYRPLMPFGTPAGRTADISRKTLRLAQALYAAETTFLDAWLGRFLTRLD
                     ELGLSNTWVAVVSDHGVSLGEHGIIGKNSNQLHGELIDVPFMIRHPQRVGAGRRSGYF
                     ASSQDIAPTLLRGVGAPVPKAMDGVDLRPLFDGRRVKQRRPVWTSAWGDTLMAGDGRW
                     LYVAQNAGGRVVTVRLHDTRSDPGEYRNLARRRPDLVRKFRDELLKGAGKQGFPSL"
     gene            complement(7905..8819)
                     /locus_tag="VHF88_03520"
     CDS             complement(7905..8819)
                     /locus_tag="VHF88_03520"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX2160869.1"
                     /translation="MPGDPVRIREVGPRDGFQNEPETIATDDKVRLVEMLARSGLRRL
                     EVTSFVRADVIPQLADGPDVLTRADIPEDVAVTVLIPNERGLDNALALRDRFQEVNLF
                     LSASESHNRANVNRSIEESLGGLEKVVTRALGEGLRCEAVISVAFGCPYEGAVPPERV
                     FEIAKRLRAAGCAELGFADTTGMANPAQVREFFAAAFERLPEVELTAHFHNTRGQGLA
                     NALAALEAGCRSFESSFGELGGCPVPRGATGNIATEDLVSMLHEMGHPTGIDLGALLD
                     CAREVQRVLGRPLGSHLLTAGPVDWQRG"
     gene            complement(8822..9568)
                     /locus_tag="VHF88_03525"
     CDS             complement(8822..9568)
                     /locus_tag="VHF88_03525"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2160870.1"
                     /translation="MRFESKVALVTGGAGGIGGATASRLAAEGARVAIGDLDAAAARE
                     LAADLGGIGLELDVRSDESIASAVEATERQLGPIDVLVNNAGVAGELFFTQTPPEVWD
                     NMLAVNLRGVLAVTHAVLPGMHERRGGAIVNVASEAGRVGSQLSVVYSATKAGVIGFT
                     KALAKESARFGVRVNAVAPGPIDTQMVRAAEVLGEIGERMVQGMIDSTVMKRLGEPEE
                     VAAAIAYLASDEASFVTGETVGVSGGLGMM"
     gene            9649..10332
                     /gene="npdG"
                     /locus_tag="VHF88_03530"
     CDS             9649..10332
                     /gene="npdG"
                     /locus_tag="VHF88_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX2160871.1"
                     /translation="MPGSPIPIVGGTGALGFGLALRLAKAGEEVVIGSRDAGRAEEAA
                     VRIRARVSNAHVAGMENAEAARLGEVVFLTVPFRAQSENLTNLKEALRDGQILVDATV
                     PLAAAISGKATRLLGVPQGSAAEQAQEMVPDGVTVISALHSVDASTLGNLDEPLDQDV
                     LICGDRKEPKRKVAELIGLIEGLRPVNAGALETARFVEALTPLLISVNIRNKSHAGVR
                     LTGLPDQLW"
     gene            10338..11249
                     /gene="cofD"
                     /locus_tag="VHF88_03535"
     CDS             10338..11249
                     /gene="cofD"
                     /locus_tag="VHF88_03535"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX2160872.1"
                     /translation="MRVALLSGGTGGAKLARGLLDEVGAEELTVIANTGDDAWVYGVH
                     VSPDPDLVTYWLADAIDERGYGIRDDSWQVMEALAAAGRPTWFRLGDRDLAMCLTRSE
                     QLAAGTRLTDAHAAVVGAMGVGARVLPMCDEPVPTRIGVGGRVLPFQEFMIVEQARGP
                     LESVAFDGVERARPTDEVLAALRDADVVVIGPSNPIVSIGPILALPGMREALIATPAP
                     VVAVSPFVGGRVLKGPTELFCRFAGVEPSATGIAGLYAGLIDGLVADEPVEGLPAAEV
                     PTLMDGPASRRQLARATLELATTLSTS"
     gene            11303..11995
                     /gene="cofC"
                     /locus_tag="VHF88_03540"
     CDS             11303..11995
                     /gene="cofC"
                     /locus_tag="VHF88_03540"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX2160873.1"
                     /translation="MRTIAILPIKSFDAAKQRLAGLLGSGARQALVQAMFQDVLSSLR
                     HVESLDAIAVVTANPVAARAARGEGVVVLGDSEETGHNDAAGLGIAYAVEHGFERVML
                     VPGDTPLLDPDEIEGVLRDREAGLVVVPDRHGSGTNALLIAPPGAVAPSFGAGSRRRH
                     ELLAAAAGVPCSVVPLRSLAVDVDTPEDLDELAALLGDAANRSRASMTRGALRQLDRS
                     GAAPSLPSAAIS"
     gene            12010..12753
                     /gene="cofE"
                     /locus_tag="VHF88_03545"
     CDS             12010..12753
                     /gene="cofE"
                     /locus_tag="VHF88_03545"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX2160874.1"
                     /translation="MSAVALDGIGEVRAGDDLAALVAAALPDDLGAGDIVAVAHKVVS
                     KAEGRTRRLADIEPTRRARDLATQHGKDPRVVQAVLDESARVLRAGHGVLICETHHGL
                     VCANAGVDRSNSAEPDQLILLPLDPDASARALRAGIGALRGIAPAVVVTDSFGRAWRL
                     GQTDVAIGSAGVTPLDDWRGRRDAHGRELQATAIAVADAIAGAADLVRAKDANQPAVL
                     LRGLDRFVTTGDGPGAAALRRPAADDLFR"
     gene            complement(12767..13555)
                     /locus_tag="VHF88_03550"
     CDS             complement(12767..13555)
                     /locus_tag="VHF88_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2160875.1"
                     /translation="MEVPRDLLGSLGDIAKFCWRVLVEVFEGRTIKFFGETLRQAGIL
                     IIGSGLVIWGLAFILGLSICGVEAAYFNRSIGAPAYAGWFGAWCDLREAMPYAFGYMM
                     SAKVGTGLVAEIGAMRINEEIDALDVMGISSLTFLCSTRLLAAWVVLPFMYIIAVGVG
                     FFATHLAIVEQIGEVSSGGYSLIFWMFQNPPDLFFSTIKGMAMATVIVLVGCYYGYTA
                     SGGPVGVGTATAKSMVLNTVLVHILGMLGTQIFWGTNPRAPIGG"
     gene            complement(13570..14385)
                     /locus_tag="VHF88_03555"
     CDS             complement(13570..14385)
                     /locus_tag="VHF88_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2160876.1"
                     /translation="MVAGVYPVVRRAYLPVKNGLEQTGEMMILTGKTLVSAIRPPHPY
                     GTEFVQQFMFVLRLCWFPLLISTVAICYGAPGLQAGNALVIFGALDRLGGFFVLASIR
                     EIGPNVTAIVVAGVGGTAITADLGARKIREELDALQVLGVDPVKNLVVPRFLALMIIT
                     GLFNIFAVMFGLFGGVLATITFDAPLGPFFATLLSNASTTDLWGSVLKTTLFGAIIAI
                     VSCYKGMTASGGAEGVGRAVNQGVVIEFLAIFAFNYVFTQTLLATHPEISVIK"
     gene            14425..14982
                     /locus_tag="VHF88_03560"
     CDS             14425..14982
                     /locus_tag="VHF88_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2160877.1"
                     /translation="MQLQLDRDSGVPMGTQLVWRLRSAIDRGELRPGDKLPSLREAAT
                     AAGVNVNTVRAAYAKLESAGVVTTEQGRGTFVAGASDLASARRRALRDDIARLEAELV
                     RLPLYPQAAAEPAVRNAPGARLLSVEELETVRDVLAERVGELHAARAAVVARLEGERR
                     ADATPRRTPARRSSSSLAGARVRWT"
     gene            complement(15014..17203)
                     /gene="cofH"
                     /locus_tag="VHF88_03565"
     CDS             complement(15014..17203)
                     /gene="cofH"
                     /locus_tag="VHF88_03565"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX2160878.1"
                     /translation="MRRVTFSRNFTISLSRTCRCYCKYCAFATHRAHLYSPEEVDGLI
                     EEAVRRRAKELLVLTGERPEVNAEVRARLRVYGHDDFVSYVVWTCERALERGLLPHTN
                     LGVLSKEDLGRLREVTASQGLMLESVNPDLVVHQGSPTKHPQRRLETIRAAGELRIPF
                     TSGILVGIGETPRQRVEALEALAAVHADHGHLQEVILQNFVPHQRYYGAEPAEIADEA
                     QLSGSAGEPAALPSWATAVTLDDMRELVGVARELMPGVGVQIPPNLSDWWLPLIEAGA
                     TDLGGLSANGDHISPEHPFPTPQRMRKTLEPRGYALAERLCVYPQYMDADWVEEGVLD
                     TIKTAYWSFIPRRGSGRREERAIRRDLVPGAVERARDGQPLDEHELTALFAETRPEAI
                     EDIRQAAHELRAELAGETVTFVVNRNINVSNVCTVGCAFCGFGQGRRSPDAYEHDREE
                     FVRRVSEAVEFGATEICMQSGIHPDWGLEDYEGWLRLAKQVAPQIHLHAYSAMEVDAM
                     CATSGLAPREVFERLRAAGLDSTPGTAAEVLHDGVRERISPNKLPVARWVEIIEASHA
                     AGLRSTVTVMFGHIEEPWELAEHMRVVRELQERTGGFTEFVPLSFVPYHTLLGRTHGI
                     EELSRDENLKHTAVFRLALGRTIPSLQASWVKMGLDAATESLRWGVNDLGGTLMEESI
                     SRLAGSRHGVMLTPEQLIDAAHAAGRPAAERTTLYDIVRRFELPLAA"
     gene            17250..>18034
                     /locus_tag="VHF88_03570"
     CDS             17250..>18034
                     /locus_tag="VHF88_03570"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2160879.1"
                     /translation="MRLPAILTLLALLGLPLAACGEDGNSRDDDARPLTLALDFQPNA
                     VHAGIYTALARQLDRRHGIDLDVRVPSSSTDSLKLLAAGRADAAIVDIHDLGLADQRG
                     ADLVGIGALVQRPLAAVIAHPSIRRPRDLEGRRVGVTGLPSDDAVLRATVEADGGDYD
                     AVDKITIGFSAVPALASNRVAAVVTFWNAEGVALRHENIPTRQFRVDRYGAPPYPELI
                     LATTPHNLSANPQLFEDLLTTLTEGTTAALADRPTAVHEVATAS"
BASE COUNT         2887 a         6466 c         5950 g         2731 t
ORIGIN      
        1 gtcggcgcgg gagccggagc cggcctcggc ggcggcttcc gacgccgcgg cgacgggacg
       61 cgcgggggcg cctgcgaagc cggcggcgtg gccggagcgc cgccggaggt tgggggtggt
      121 ggatcgggct caggcgtgcc gcgcgggact ggccctgcag cgggcggagc cgcccttccc
      181 cgaccgcgcg agcgatccct agccgctccg ttgcgacgcg gctcagtgcg ctggcgctcg
      241 ccgccctgct cgcgcagctg gatcggcgcc accgacggcg cgtcgaggtt cccggcgagc
      301 ttcggcagca cggcgagcag gaccaccgtc acgacgacga gggcgagcgc cagcagacgt
      361 tgcgcgagcg acaccctcac catcctgaga gcccgcgctg aggccagcat gagagccgtc
      421 taagacttct ctcacggaag agtccggggg cgcgagcccc cgtccctcct cccaccggcc
      481 aggcgccccc ggtggctaca cctttgggtt ctggactggc tcgtcgcccg agtagtcgta
      541 gaagcccatg ccggacttgc ggccgtacca gccggcggcg agcatcttgc gcagcgtcgg
      601 cgggcgggcg aagcggcgct cgcggaactc gtcgaagagc acgtcgcaga ttgagccgag
      661 cgtgtcgagg ccgacgaagt cggacagcag cagcgggccc atcgggtggc cggcgccggc
      721 cttcatcgcg gcgtcgatct cctcgatcga gccgacgccc tcctcgtaag cgcggatgcc
      781 gtcgagcagg tacggcacga gcaggcggtt gacgatgaag cccgcggtgt cgcgcgtcgg
      841 gatgccgagc ttgccgaggc tcttggtgaa ctcgacccct gcggcgaagg tctcgtccga
      901 ggtcgtgatg ccgcggacga cctcgacgag cttcatcacc tgggcagggt tgaagaagtg
      961 caggccgagg aagctctcgg gccgcgaggt cgaggcggcc tgatcgatca ccgacagcga
     1021 cgacgtgttg gtcgcgaaca gcgcgccctg cttgacgatc ccgtcgacct ccttccacat
     1081 ctcgagcttc agcgcgaggt cctcggtgat cgcctcgatc accagctcgc agtccgcgag
     1141 gtcggagtag gaggtcgtgc cgtggatccg gccgcggacc gcgtcggcgt cggcctgctc
     1201 ggccttgccc ttctcgacgg cgcgcgcgag ctgcttctcg atcttgccga cgcccttctc
     1261 gagcttggcg tcgtcgacct cgcgcagcac gacgtcgtag cccgcctgcg cgaccacctg
     1321 ggcgatcccg tggcccatca ggcctgcccc gagcactcca accttcttga tctccactac
     1381 gttctcccag tcagctgtca gcgagcccgg ggagcctata tgtcactgat cggagtcgag
     1441 gaccgtggcg gcgtgcgtca tctggtgctg cagcgggccg agaagcgcaa cgcgttcaac
     1501 cgcgagctga tcgaggcgct cgggcacgcc ctcgaggacg cggcggccga cacgtcggtg
     1561 cgcgtgctgg tcgtgcgcgg cgacggggcg atgttctcgt ccggcatgga cctcaacgac
     1621 ctgcgcgagc tcgccggcga cgcatcgcag ctgcgaccgt tccgccgcgc gatcctcggc
     1681 tggtggaacc tgctcgagga gatgccgaag ccgacgatcg tccagatcca cggcgcctgc
     1741 ctcggcggcg ccttcgagct cgcgctcgcg gccgacttcc gcgtgatggc tgaggacgcg
     1801 atggccggga tcatggaggt ccgcgtcggc ctgcttcccg acgtcggcgg ctgctcgcgc
     1861 ctgccgtcga tcgtcggtct cggcaacgcc aaggagctga tcatgaccgg caaggtgatc
     1921 gacggacgcg aggcgcaccg gatcggcttc gccaaccgga tcgccccaac cgaggagctc
     1981 gacgcgctga ccgaggcgtt cgcgaacgag ctgctcgcct gcgcgccgcg cgccgtcgga
     2041 atggcgaagc gcgtgatcga cgcctccgcc aagcccgcgc tcgccgcgac gctcgagcag
     2101 gaggtccagg cccaggaggc actcgtcgcc agcgaggact tcgccgaggg cacgcgcgcc
     2161 ttcttcgaga agcgccagcc aaacttcggg gccaagtaag ccgcacgcac gcatcggccc
     2221 taggttggtg ttctagcacc gaccgccgac cactcgagga ctccagccta cgttcccttc
     2281 cggttctcct agtaaccggg taacgcgcga ctcaatcgtc tcaggtcgct ttgaggcccc
     2341 gggcccgtac tcgtcgcctt agtccggtgc gtcgaatagt tcgagttcct tccacctttg
     2401 aggctcttgg agtgcggctc gtcgccgcaa gctggtctcg gcgatcaagt gctcaagaac
     2461 cgcctcgctc acattgcccg ggacgcccac gtgagaatcg aagtcaagca ccaacgcgcc
     2521 cgcatccctg tctccaacaa cggtacgcag agcgtcctca agtaggggga tccatccggg
     2581 ctccgccgac atagcgtcga tgacttccgc tgcggatggc atcggctcag accgggccag
     2641 tatttgagta atcgacgcag cctgcgcgga aagcttccac cggcgctgta catcgtcagg
     2701 ggcttcccac ccggtatacc cagactgcgc cattgccgca gcgagcaacc tccacgcccc
     2761 cataagaccg ggacgtgccc ggacgagacc tgtgagcgtc tcgatgatca cggtgtggtc
     2821 ggcgaaagcg ccgcgcgcac tagggctacg acgcccgagt tcgtcaagag cgtttgccaa
     2881 gtcgaccggg ttctcgtaac cgtcgagata gtgcagaaca cccacttggg cgccctctga
     2941 ccaggtgggg ggctcggtgg cgatcctgag gccaccgcta ttctcgacca agatctcaaa
     3001 gtcgttcagg cctattagca aaccgtcacc ttcatcaggg cggtctggga tcaggctggc
     3061 aatcgccagg gcccaacggg ctaacacttg tttgccgccc gctggtgccg gaacgtgccg
     3121 ccaggcgtcc acgacgttat atgcggtacg tggactcgca acgttccaac cgcatccgta
     3181 gagtggaatt ccccctttcg ctacggacgc gtcgaaaggg agaccatcct cccagaaatg
     3241 accaatgtcc tccgtagtga cacccccgtc taaccagcgc tgctccgctg cccgccactg
     3301 atcgatattc cccatacggc cggctcgggc cgcctcaatg atctcttccg cctgagcgcc
     3361 attagctatc gcgcccagac aagcacccag cggccacggc ccggtctggc cccaccgata
     3421 tggcaagagc tcatgcgtca tgatctcatc aattgcgtca cccagagcag tgcatgacgg
     3481 attattgacg aactcgccaa caatcgcctc ataccgccat gcgggatgca ctacatccac
     3541 attagggact tccttcaatg aagcatttaa gctgggatcc tcagaggtga cacgggtcat
     3601 agttagccgg aaactgctgg gctcgacacc cgcgatcggt atggcgcttc cgaaactatg
     3661 tttcgccctc cgaacacctc gagccaatag atttgcccag ggaacatccg ctttagccgc
     3721 catctccagc atcgcgacat atcgctcgtc gtacccgatc ccatgtcgta gagccgcgct
     3781 ggccaggacc tgcgagacgc gcgcgccaat ttcgagcggc ccatccatct cgccaagtaa
     3841 cgctgcctcg ctagcgccgc cgttgtcggg ccacgcttca aggagcattt gttcagccca
     3901 atgaacgcca ccgtctacca agctcagtag caggcgccat gtcccagatc gcacgacggg
     3961 tacgcggagg cccgcctcca aagcgtcccg aaacgcgctc tgagagcggt catcgtagag
     4021 tcgggcaagc cgggcttgca agtcggacgc cggcctatcg acacactcca atgcctgctc
     4081 caagagcaat cgccggtaac gcggctggtc gcgggccgac ccgtcctcag acagatcaat
     4141 cgcgagtgac gcgcccgtag ccgcggcctt cgacactccc gcatcctcaa gatcgctgtt
     4201 ttgctcagca caaatggtat atacggtgtc acgaaggtgc tgccgatcag cgaagcacct
     4261 ccccgcagcg aacaagaaga cattccgcca cgcgctcgct gaggcaatcg acctcagccg
     4321 cccggcgacg acgtcatcac caccgtccat gagcgcctca gcggccatga actcttgcaa
     4381 ggagcgtatc tcaaatccaa cgcggtcctc ctcgactcca accacgaaga cgaggcgctc
     4441 ggcagccgca gcgagaatcg agtcacacaa ctcctgcaat tcccgcccct cataggcctc
     4501 ctcctctaac cgcccgcgaa cgattgaggc gaacctgtcg cgtgacaaat gggactcggt
     4561 gttacgggcc acctcgctat ctctctgtaa gacaagggct acacgccggt gaatgcgatc
     4621 gacgttatgc ttttgacgcc gaaggatgtc agcggccggt atgtcgcgct ccatctcacg
     4681 gttatagatt acgctgtagt actcagaaaa caaactccac cgttgctcgg gcggctgacc
     4741 catctgatcg acgagtgtcg ccatgatcgt gacctggaga gggctaagca tgagccgcgc
     4801 cgtagtctcg acgcgtgcgg cacgctcaag gcgtccgata acccgctcac accggtctcg
     4861 atctgcgcca tagcgagcct cggccagctg tcttccgtaa cttagggctt tagcctgccc
     4921 gagtggggca agagtacgat gcagatagag actgggggcg aaatcgtcgt tgtatccttg
     4981 aggcctcgtc gttgcgagca cgagtgcgtc agtctcactg tctgcaatgt ctacccaaaa
     5041 ctcgttgatc gcggtcagga ctgcgtctcg gttgctagaa ctaggcacct cgtcgagacc
     5101 atcgaggaca attaaccacg ggtagccgtt gagccactgc ctgaacgcat cgatcgggac
     5161 gtcacggtcg atccgttgct cgacccgtcg agtaataaag cggagcaagg atagatttcc
     5221 atcctcctcc tccaacgcgg tagcgaactc gctcaggacc actcgaatcg gaaaccgccg
     5281 tacgcctggg cccgatattt gcgatgcgtc acagtgccga tctatgagcg ccatagcggt
     5341 ccgggcctcg gccgagattg tctgcggcgg tctttcggct agaagcgagg cccggaacag
     5401 ctggcatacg aactggccta gagttgtctt tccctggcct ggcccgccta tcaagaccca
     5461 ccgcccgggc ttgctgctga cttgggtggg atgccttccc tcagatgggc ctagcgatcg
     5521 tgctgatcca ggattcaact tgacgtgcgc agcatcaaga agttgctcca ccaaaccgcg
     5581 gggctcggag gcctcatcca cgaaatggtc ctcggtctca aggagcggaa ggtcaacaaa
     5641 gacgcgtgcg atgggaaccc gctcctccgg tgcacgacca gcctgctcaa gatttgcgta
     5701 ctgatcagcg aggagctcct tctgcagaaa cagtgacagg gtgcgatcaa aatcgggggc
     5761 agagattgcc aagtcatcaa ccacctttgc tagcacgtca cccgccgtga tccacgcagc
     5821 gtaggcgtta cgcaaatcgc tgtggagatc gagaaagcga cacagcttgt ctcggtccca
     5881 aacgtcgtac ccctttagaa gtttgctgtt gaccgccgaa cgtaagatgg cgtctacctt
     5941 gtcctttgac ccctgctttt gcacaggagt caacgtgacg tttgtaacgt acaggtaata
     6001 gtctggtcgg tccccggtaa agcctgaggc ggtgtagcgg tccatctcct tctgaagctg
     6061 ctcagcagcc cattgtccgt cgcgaccccg atcagacagc acctgacgaa acttggcttg
     6121 taccaccagg tagccgtccc agctaccgtc tcccagacca gggattttac ccttgaacgc
     6181 tgcctctcga ccgccgtctg gcccatcgcc aaaaataact acactcggcc cgatttcggc
     6241 gatcgcaatg ctttgaatca gctgctcgaa agagcgagac gaaagcccct ctagccggta
     6301 gtccacgcga ggagactatc gagaagctag agagatggaa acccctgctt acccgcgccc
     6361 ttcaagagct cgtcacggaa cttgcgcacc aggtccggtc ggcggcgagc gaggttgcgg
     6421 tactcgccgg ggtcgctgcg cgtgtcgtgc aggcggacgg tgacgacccg cccgccggcg
     6481 ttctgggcga cgtagagcca gcggccgtcg ccggccatca gcgtgtcgcc ccaggccgac
     6541 gtccagaccg ggcggcgctg cttgacgcgg cggccgtcga acagcgggcg caggtcgacg
     6601 ccgtccatcg ccttcgggac cggggcgccg acgccgcgca gcaacgtcgg cgcgatgtcc
     6661 tgcgacgagg cgaagtatcc gcttcgccgc ccggcgccga cgcgctgcgg gtggcggatc
     6721 atgaacggca cgtcgatcag ctcgccgtgg agctggttgg agttcttgcc gatgatgccg
     6781 tgctcgccga gcgagacgcc gtggtcggag acgaccgcta cccaggtgtt cgacagcccg
     6841 agctcgtcga gccgggtgag gaagcggccg agccaggcgt cgaggaaggt cgtctcggct
     6901 gcgtacagcg cctgcgcaag cctcagcgtc ttgcgcgaga tgtcggcggt gcggccggcg
     6961 ggcgtcccga acggcatcaa cggccggtag ccgccgcgct tgattcgcgc gtagcgcgcg
     7021 aggtagctgt cgggcgggtc ccacggctcg tggacgtcga agcagtcgac gaccagcgcg
     7081 aacggctgcg cgcggaacgc tgcctgctcg aggaagtcga tgccgcctcc gaagacgcgc
     7141 gccgcgaggt agtcgcgctc acggcggctg gcgccgttgt aggcgaggta ctcgcgcacg
     7201 cgcccgcggt cgccgccgcg caactcgggc gcgaggtgac gccgcaggcg gcctggtgac
     7261 accggtctgc ggcgctggcg ccagccgggg atctggccct cgacgaccgt catcacgtcg
     7321 ggcctgccac ggaagcgcgc gaacgcggga cgcagcaggt gcgggttgtc cgtcacgtag
     7381 ccggtcaggt agccgcgctt gccgagcacg tcgaggaacg tcgtctcgcc gcggtggatg
     7441 ccctcgaagc ccgggaccgc tggaagcgct ccctccgggc gccagccgcg aaacggaaag
     7501 ccgctgcggc cgatcagcag cgacttacgg accggcaccg tcggcatcgc gtcgtggcgg
     7561 gcgtgggtga agagcaggct ctcggtcgcg agcgcgtcga gcgttggcgt ccgcgcccgc
     7621 cgcccaccgt aggcgccgac gtggtcggtg cgcaacgagt cgatcacgac gacgacgacg
     7681 ttggcgttcg ccgcggggat cagccccggc gccagcgccg cggcctcgct gcggccggcg
     7741 gccaggcccg gcacggcggc gcccaacccg agcgcagcgg cgccaccgag cagccgccgc
     7801 cgcgtcagct cgctagcgag ttcttcacca gctcggtgag aatccatatc cgcagccaca
     7861 gtagcggtga acccggcggg agcgcgttag ttgctggccc agcgtcagcc gcgctgccag
     7921 tcgaccggac ccgccgtcag aagatggctt ccgagcgggc gcccgagcac gcgctgcacc
     7981 tcgcgcgcgc agtcgagcag cgcgccgagg tcgatgccgg ttggatgtcc catctcgtgg
     8041 agcatcgaga cgaggtcctc ggtggcgatg ttgccggtcg cgcccctggg caccgggcag
     8101 ccaccgagct cgccgaacga gctctcgaac gagcgacagc cggcctcgag cgcggcgagg
     8161 gcgttggcca ggccctggcc gcgcgtgttg tggaaatggg cggtcagctc gacctcgggc
     8221 agccgctcga aggcggcggc gaagaactcg cgcacctggg ccgggttcgc catgccggtc
     8281 gtgtccgcga agccgagctc ggcgcagccg gcggcgcgca ggcgcttggc gatctcgaag
     8341 acacgctcgg gtggcaccgc gccctcgtag ggacagccga acgccaccga gatcaccgcc
     8401 tcgcagcgca gcccctcgcc gagcgcgcgg gtgaccacct tctccaaccc gccgagcgac
     8461 tcctcgatcg accggttgac gttggcgcgg ttgtggctct ccgacgccga caggaagagg
     8521 ttgacctcct ggaagcggtc gcgcagcgcc agtgcgttgt cgagcccgcg ctcattgggg
     8581 atcagcacgg tgacggcgac gtcctccggt atgtcggcgc gcgtcaggac gtcgggcccg
     8641 tcggcgagtt ggggaatgac gtcggcgcgg acgaagctcg tcacctccag ccggcgcagg
     8701 ccgctgcgcg ccagcatctc gacgaggcgg accttgtcgt cggtcgcgat cgtctcgggc
     8761 tcgttctgga agccgtcgcg cggaccgacc tcgcggatgc gaacgggatc tccaggcatg
     8821 cctacatcat gccgaggccg ccgctgaccc cgacggtctc gcccgtcacg aaggaagcct
     8881 cgtcgctcgc gaggtaagcg atcgcggcgg cgacctcctc cggctcgccg agccgcttca
     8941 tcacggtcga gtcgatcatc ccctggacca tacgctcgcc gatctcgccg agcacctcgg
     9001 cggcgcgcac catctgggtg tcgatcggcc ctggcgcgac ggcgttgacg cggacgccga
     9061 agcgcgccga ctccttcgcg agcgccttgg taaagccgat caccccggcc ttggtagccg
     9121 agtagacgac agagagctgc gagccgacgc gcccggcctc ggacgcgacg ttgacgatcg
     9181 cccctccgcg gcgctcgtgc atcccgggca gcaccgcgtg ggtgacggcc agcacgccgc
     9241 gcaggttgac cgcgagcatg ttgtcccaga cctcgggcgg ggtctgcgtg aagaagagct
     9301 cgccggcgac gccggcgttg ttgaccagca cgtcgatcgg cccgagctgc cgctcggtcg
     9361 cctcgaccgc cgacgcgatc gactcgtccg agcggacgtc gagctcgagc ccgatcccgc
     9421 cgaggtcggc ggcgagctcc cgcgcggctg ccgcgtcgag atcgccgatg gccacgcgcg
     9481 cgccctcggc ggcgagccgc gacgccgtcg cgccgccgat cccgcccgcc ccgcccgtca
     9541 cgagcgccac cttgctctcg aaccgcatcg gcgagaccct atccctgcgc gtgcctgcgc
     9601 cggctgagga ctgacggccc gcgggcggct gttagcgtgc cgcgccggat gcctggctcc
     9661 cccataccga tcgtcggcgg caccggcgcg ctcgggttcg ggctggcgct gcggctcgcc
     9721 aaggcgggcg aggaggtggt gatcggctcg cgcgacgccg gacgggccga ggaggccgcc
     9781 gtgcgaatcc gcgcccgggt gtcgaacgcc cacgtcgccg gcatggagaa cgccgaggcc
     9841 gcccggctcg gcgaggtcgt ctttttgacc gtcccctttc gcgcccagtc cgagaacctg
     9901 accaacctca aggaagcgct ccgcgacggg cagatcctcg tcgacgccac cgtgccgctc
     9961 gccgcggcga tctccggcaa ggccacgcgc ctgctcggcg tcccgcaggg ctccgcggcc
    10021 gagcaggccc aggagatggt ccccgacggc gtgacggtga tcagcgcgct gcactcggtc
    10081 gacgcgtcga cgctcggcaa cctcgacgag ccgctcgacc aggacgtcct gatctgcggc
    10141 gaccgcaagg agcccaagcg caaggtcgcc gagctgatcg gcctgatcga ggggctgcga
    10201 ccggtcaacg ccggcgcgct cgagacggcg cgcttcgtcg aggcgctcac gccgctgctg
    10261 atctcagtca acatccgcaa caagtcgcac gccggggtcc ggctgaccgg cctgcccgac
    10321 cagctctggt agctccgatg cgcgtcgcgc tgctctccgg cggcaccggc ggggcgaagc
    10381 tggcgcgcgg cctgctcgac gaggtcggcg ccgaggagct gaccgtgatc gccaacaccg
    10441 gcgacgacgc ctgggtctac ggcgtccacg tctcccccga ccccgatctg gtcacctact
    10501 ggctcgccga cgcgatcgac gagcgcggct acgggattcg cgacgacagc tggcaggtga
    10561 tggaggcgct ggcggcggcc gggcggccga cgtggttccg gctcggcgac cgcgacctgg
    10621 cgatgtgcct gacccgcagc gagcagctcg cggcggggac gcggctgacc gacgcccacg
    10681 ccgcagtggt cggggcgatg ggggtcggcg cgagggtgct gccgatgtgc gacgagccgg
    10741 tcccgacgcg gatcggcgtt ggcgggcggg tgctgccgtt tcaggagttc atgatcgtcg
    10801 agcaggcgcg cggcccgctc gagagcgtcg ccttcgacgg cgtcgagcgg gcacggccga
    10861 ccgacgaggt gctggcggcg ctgcgagacg ccgacgtcgt cgtgatcggg ccctcgaacc
    10921 cgatcgtctc gatcggcccg atcctcgcgc tgccgggcat gcgcgaggcg ctgatcgcca
    10981 cgcccgcgcc cgtggttgcc gtcagcccgt tcgtcggcgg ccgggtcctg aagggaccga
    11041 ccgagctgtt ctgtcgcttc gccggcgtcg agccgtcggc aaccggcatc gccggcctct
    11101 acgccggcct gatcgacggc ctggtcgccg acgagcccgt cgaaggccta ccggccgccg
    11161 aggtcccgac gctgatggac ggcccggcga gccggcgcca gctggcgcgg gcgacgctcg
    11221 agctcgccac gacgctgtcg acgagctgac cgatatctcc gcgcaccgcg gttagagtcg
    11281 ggccagccga cccgatcgcc cgatgcgaac gatcgcgatc ctcccaatca agagcttcga
    11341 cgccgccaag cagcggctcg ctggcctgct cggaagcggc gcccgtcaag cgctcgtgca
    11401 ggcgatgttc caggacgtgc tgtcgtcgct gcgccacgtc gagagcctcg acgcgatcgc
    11461 cgtggtcacc gccaacccag tcgctgcacg cgccgcgcgc ggcgaggggg ttgtggtgct
    11521 cggggacagc gaggagaccg gccacaacga cgccgccggt ctggggatcg cctacgccgt
    11581 cgagcacggc ttcgagcgcg tcatgctcgt gcccggcgac acgccgctcc tggacccgga
    11641 cgagatcgag ggcgtgctgc gcgaccgcga ggccggtctg gtcgtcgtcc ccgatcgcca
    11701 cggctcgggc acgaacgcgc tcttgatcgc tccgccgggc gccgtcgcgc cgtcgtttgg
    11761 agcgggcagc cgccgacgcc acgagctgct ggcggccgcg gcaggagtgc cctgctcggt
    11821 cgtcccactg cgctccctgg ccgtcgacgt cgataccccg gaggacctcg acgagctcgc
    11881 cgctctgctc ggcgacgccg ccaaccgctc cagggcgagc atgacgcgtg gcgccctgcg
    11941 ccagctcgac cgctccgggg ccgctccctc cctcccctcc gccgcgatct cctgaaccag
    12001 ctgcgcgtcc tgtccgccgt cgcgctcgac ggcatcggcg aggtgcgagc cggcgacgac
    12061 ctcgccgcgc tcgtcgccgc cgcgctgccc gacgacctgg gggccggcga catcgtcgcg
    12121 gtcgcgcaca aggtcgtctc gaaggccgag ggtcgcacgc gtcggctcgc tgacatcgag
    12181 cccacgcggc gggcgcgcga cctcgccacc caacacggca aggacccgcg cgtcgtccag
    12241 gcggtgctcg acgagtcggc gcgggttctg cgggccggcc acggcgtgct gatctgcgag
    12301 acccatcacg gcctcgtctg cgcgaacgcc ggcgtcgatc gctcgaacag cgcggagccc
    12361 gaccagctga tcctgctgcc gctcgacccc gacgcttccg cgcgcgccct gcgtgccggg
    12421 atcggggctc tgcgcggaat cgcgccggcc gtggtcgtaa ccgactcgtt cggccgcgca
    12481 tggcggctcg gccagaccga cgtcgcgatc ggcagcgcgg gcgtgacccc gctcgacgac
    12541 tggcgcggcc gccgcgacgc gcacgggcgc gagctgcagg ccacggccat agccgtcgcc
    12601 gacgcgatcg ccggcgccgc cgacctggtc cgtgccaagg acgccaacca gcccgcagtg
    12661 ctgctgcgcg gcctcgaccg cttcgtcacg accggcgacg gccccggcgc agccgcgctt
    12721 cgccgcccgg ccgccgacga cctgttccgc tagccgggcg tagcggtcag ccgccgatcg
    12781 gcgccctcgg gttggtcccc cagaagatct gggtgccgag catgccgagg atgtggacca
    12841 gcacggtgtt gagcaccatc gacttcgcgg tcgccgtccc gacgccgacc ggcccgccgc
    12901 tggccgtgta gccgtagtag cagccgacga gcacgatgac agtcgccatc gccatgccct
    12961 tgatcgtgct gaagaagagg tctggagggt tttggaacat ccagaagatc agcgagtagc
    13021 cgcccgacga cacctccccg atctgctcca cgatcgccag gtgggtggcg aagaagccga
    13081 ccccgacagc gatgatgtac atgaacggca ggaccaccca ggccgcgagc agccgcgtcg
    13141 agcagaggaa cgtcagcgag ctgatgccca tcacgtcgag cgcgtcgatc tcctcgttga
    13201 tccgcatcgc gccgatctcg gcgaccaggc cggtccctac cttcgccgac atcatgtagc
    13261 cgaacgcgta gggcatcgcc tcgcgcaggt cgcaccacgc gccgaaccag ccggcgtagg
    13321 cgggcgcgcc gatcgagcgg ttgaagtagg cggcctcgac cccgcagatc gagagcccga
    13381 ggatgaacgc gagtccccag atcaccagcc ccgagccgat gatcaggatc ccagcctgcc
    13441 gtagcgtctc gccgaagaac ttgatggtgc gcccctcgaa cacctcgacg agcacgcgcc
    13501 agcagaactt ggcaatgtcg cccagcgacc cgagcaggtc gcgcgggacc tccatccagg
    13561 aggcgcccct cacttgatca ccgagatctc gggatgggtg gcaaggagcg tctgggtgaa
    13621 gacgtagttg aacgcgaaga tcgccaggaa ctcgatcacg accccctggt tgaccgctct
    13681 accgaccccc tcagcgcctc cggacgcagt catccctttg taacaagaga cgatcgcgat
    13741 gatcgcgcca aacaacgtcg tcttcagcac cgagccccag aggtcggtgg tcgaggcgtt
    13801 gctgagcagc gtcgcgaaga acggtcccag cggcgcgtcg aaggtgatcg tcgcgagcac
    13861 gccgccgaac aggccgaaca tgacggcgaa gatgttgaac aggccggtga tgatcatcag
    13921 cgcgaggaag cgcggcacga cgaggttctt gaccgggtcg acgccgagca cctgcagcgc
    13981 gtcgagctcc tcacggatct tgcgcgcacc gaggtcggcg gtgatcgccg tcccgccgac
    14041 acctgcgacg acgatcgccg tgacgttcgg cccgatctcg cggatcgagg ccagtacgaa
    14101 gaagccgccg agccggtcga gcgcgccgaa gatgaccagc gcgttgcccg cctgcagacc
    14161 gggcgcgccg taacagatcg cgacagtcga gatcagcagc gggaaccagc acagacgcaa
    14221 cacgaacatg aactgctgga cgaactcggt cccgtagggg tgcggcggtc gaatcgccga
    14281 cacgagcgtc ttccccgtca ggatcatcat ctcgccggtc tgctcgaggc cgttcttgac
    14341 cgggagatag gcccgtctga ccaccggata gacgcctgcc accatctgtg tggtgttcta
    14401 gcacagttcg ctagaattgc ggcaatgcag ctccaactcg atcgcgacag cggcgtgcca
    14461 atgggcacac agctggtctg gcggctgcgc tcggcgatcg accgcggcga gctgcgcccg
    14521 ggagacaagc tgcccagcct gcgcgaggcc gcgactgccg ccggcgtgaa cgtgaacacg
    14581 gttcgggcgg cctacgcgaa gctggagtcc gccggcgtcg tcaccaccga gcagggacgc
    14641 ggcactttcg tcgccggcgc gagtgaccta gcgtcggctc gccgtcgcgc gctgcgcgat
    14701 gacatcgccc gcctcgaggc cgagctggtc aggctgccgc tctaccccca ggccgctgcc
    14761 gagccggcgg ttcgcaacgc gccgggcgca cgcctgctgt cggtggagga gctcgagacc
    14821 gtccgtgacg tgctcgccga gcgggtgggc gagctgcacg ccgcgcgggc ggcggtcgtc
    14881 gcacggctcg agggcgagcg tcgcgccgac gccaccccgc ggcgaacgcc tgcgcgccgc
    14941 tcctcgtcga gtctcgccgg cgctcgcgtc cgctggacgt gatccccagc tgcggtactc
    15001 gccagtcctc ttactaagca gccagcggga gctcgaaccg acggacgatg tcgtagagcg
    15061 tcgtccgctc ggcggcgggt cgtccggcgg catgggcggc gtcgatcagc tgctccggtg
    15121 tgagcatcac gccgtgccgg ctgccggcca gccggctgat gctctcctcc atcagggtcc
    15181 ctccgaggtc gttgacgccc cagcgcaacg actcggtcgc ggcgtcgagt cccatcttca
    15241 cccacgacgc ctgcaggctc gggatcgtcc gcccgagcgc gagccggaag acggcggtgt
    15301 gcttgaggtt ctcgtcgcgc gacagctcct cgatcccgtg cgtgcgcccc agcagcgtgt
    15361 ggtacgggac gaacgacagc ggcacgaact cggtgaaccc gccggtccgc tcctgcagct
    15421 cgcgcacgac gcgcatgtgc tcggcgagct cccacggctc ctcgatgtgg ccgaacatca
    15481 ccgtcaccgt cgagcgcagc cccgccgcgt gcgacgcctc gatgatctcg acccagcgtg
    15541 cgaccggcag cttgttcggg gagatccgct cgcgcacgcc gtcgtggagc acctccgcgg
    15601 cggtgccggg cgtcgagtcc agtccggccg cgcgcagccg ctcgaagacc tcgcgtggcg
    15661 ccaggcccga ggtcgcgcac atcgcgtcga cctccatcgc cgagtaggcg tgcaggtgga
    15721 tctgcggtgc gacctgcttc gccaggcgca gccagccctc gtagtcctcg agcccccagt
    15781 cggggtggat cccggactgc atgcagatct ccgtggcgcc gaactcgacc gcctcgctga
    15841 cccgtcggac gaactcctcg cggtcgtgct cgtaggcgtc cggcgagcgg cggccctggc
    15901 cgaaaccgca gaaggcgcag ccgacggtgc agacgttcga cacgttgatg ttgcggttca
    15961 cgacgaacgt cacggtctcg ccggccagct cggcgcgcag ctcgtgggct gcctggcgga
    16021 tgtcctcgat cgcctcgggc cgcgtctcgg cgaacagcgc cgtcagctcg tgctcatcca
    16081 acggctggcc gtcgcgggcg cgctcgaccg cgcccgggac gaggtcgcgg cggatcgccc
    16141 gctcctcgcg ccgccccgag ccacgccgcg ggatgaagct ccagtaggcc gtcttgatcg
    16201 tgtcgagcac gccctcctcg acccagtcgg cgtccatgta ctgcgggtag acgcagagcc
    16261 gctcggcgag cgcgtagccg cgcggctcga gggtcttgcg catccgctgt ggcgtcggaa
    16321 agggatgctc aggcgagatg tggtcgccgt tggccgacag gccgccgaga tcggtcgcgc
    16381 ccgcctcgat caacggcagc caccagtccg agaggttcgg cgggatctgg acgccgacgc
    16441 cgggcatcag ctcgcgtgcg accccgacca gctcgcgcat gtcgtcgagc gtgaccgcgg
    16501 tcgcccagct cggcagcgca gccggctcgc cggcgctgcc cgagagctgc gcctcgtccg
    16561 cgatctcagc cggctccgct ccgtagtagc gctggtgggg gacgaagttt tgcaggatca
    16621 cctcctggag gtggccgtgg tcggcatgga cggcggcgag cgcctcgagc gcctcgacgc
    16681 gctgccttgg cgtctcgccg atgccgacca ggatcccgct cgtgaacggg atcctcagct
    16741 cgccggcggc gcggatcgtc tcgagccgcc gctgcgggtg cttggtcggc gacccctggt
    16801 gcacgacgag gtcggggttg accgactcga gcatcagccc ctgcgaggcg gtcacctcgc
    16861 gcaaccggcc gagatcctcc ttcgacagca cgccgaggtt ggtgtgcggc agcagccccc
    16921 gctcgagcgc gcgctcgcag gtccagacga cgtaggagac gaagtcgtcg tggccgtaga
    16981 cgcgcagccg cgcacgcacc tccgcattca cctccggccg ctcgccggtc agcacgagca
    17041 gctccttcgc gcgccgcctg accgcctcct cgatcagccc gtcgacctcc tcgggcgagt
    17101 acaggtgggc gcgatgcgtc gcgaaggcgc agtacttgca atagcagcgg cacgtccgcg
    17161 agagcgagat cgtgaagttg cgcgagaagg tgacgcgccg catcccgggc accctaacgg
    17221 ccggcgatag gcctagattg gccgcgccga tgcggctgcc cgccatcctc accctcctgg
    17281 ccctgctcgg cctgccgctc gccgcctgcg gcgaggacgg caactcccgc gacgacgacg
    17341 cccgcccgct cacgctcgcg ctcgacttcc agcccaacgc cgtccacgcc ggcatctaca
    17401 ccgccctcgc caggcagctc gaccgccgtc acggcatcga cctcgacgtc cgcgtcccct
    17461 cctcctcgac cgactcgctc aagctgctcg ccgccggccg tgccgacgcc gcgatcgtcg
    17521 acatccacga cctcgggctc gccgaccaac gcggcgccga cctcgtcgga atcggcgccc
    17581 tcgtccaacg ccccctcgcc gccgtcatcg cccacccctc gatccgccgc ccccgcgacc
    17641 tcgagggccg ccgcgtcggc gtcaccggcc tcccctccga cgacgccgtc ctccgcgcca
    17701 ccgtcgaggc ggacggcggc gactacgacg ccgtcgacaa gatcaccatc ggcttctcgg
    17761 ccgtccccgc cctcgcctcc aaccgcgtcg ccgccgtcgt caccttctgg aacgccgagg
    17821 gcgtcgccct ccgccacgag aacatcccaa cccgccaatt ccgcgtcgac cgctacggcg
    17881 ctccccccta ccccgagctc atcctcgcca ccacccccca caacctctcc gcgaacccac
    17941 agctcttcga ggacctactc accacactca ccgaaggcac caccgccgcc ctcgccgacc
    18001 gccccaccgc cgtccacgaa gtcgccaccg cctc
//
LOCUS       DATABV010000087        14177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2677276, whole genome shotgun sequence.
ACCESSION   DATABV010000087 DATABV010000000
VERSION     DATABV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677276"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1225)
                     /locus_tag="VHF88_03575"
     CDS             complement(29..1225)
                     /locus_tag="VHF88_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX2160880.1"
                     /translation="MEAIEELAGALRGRLIERARRGESAGDVRAEIVALVEREAAALS
                     EAERAELRERVLLAATGLGPLEPLLADPAVDEVMVNGPDDVYVERHGRIARADARFAS
                     EAELSHAIERILAPLGRRVDEASPLCDARLADGSRVNVVIPPLSLSGPCLTIRRFRRE
                     GLSLDDLVRLGTLSRDEAALLARCVAARTSILVSGGTGSGKTTTLNALSGAIPEGERI
                     VTIEDAAELRLRQRHVVRLESRPPSLEGRGEVTIRALVRNALRMRPDRIVVGEVRGGE
                     ALDLLQALNTGHEGSLSTVHANSARDALARLETLALMAGVGLPHAVVRDQVARAVELV
                     VHQARRPDGSRVVEAVTEVVACDGRADVRAVAEGGRVGELAAGRLAARLGDAGSSAPE
                     PGPVGR"
     gene            complement(1280..1879)
                     /locus_tag="VHF88_03580"
     CDS             complement(1280..1879)
                     /locus_tag="VHF88_03580"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX2160881.1"
                     /translation="MSVIALFSAAPGAGQASADEPSVGVAGAAAAATEVTLREGDRGR
                     AVRRLQRRLGIAADGVFGPQTDRAVKRFQRRNGLVADGIVGPMTRGKLGLARFSSADV
                     QHPPKDAPASGGGSNVKLPRVLREIAQCESGGDPRAVSSNGLYRGKFQFSRPTWKAVG
                     GRGDDPAAASEAHQDRMALRLYRQQGVAPWPACGARATS"
     regulatory      complement(1910..2077)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(2163..2726)
                     /locus_tag="VHF88_03585"
     CDS             complement(2163..2726)
                     /locus_tag="VHF88_03585"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX2160882.1"
                     /translation="MGFFSREIVRRAAPLVIAGAAGAAGAMLARRRGEAQRELSVVSG
                     PPQPPAPAPEPPSPVPDPIPAPEPTPEPQPSPTPVPDPAPTPEPQPPPPTVPDPSPTP
                     SPPTPEPPTAAEADLDGAVRAALATPLQEAGGNVDVEVAGRMVVLRGRVDSPEAIREL
                     ERTAATTPGVEGVRVLLHLPGRLAKRR"
     gene            complement(2755..5079)
                     /gene="lon"
                     /locus_tag="VHF88_03590"
     CDS             complement(2755..5079)
                     /gene="lon"
                     /locus_tag="VHF88_03590"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX2160883.1"
                     /translation="METEKQTLLLVPVDDSVVFPNMTVTLVVDVGDEERVLLVPREDG
                     EFASVGTVAEVSEHVRLPGGGHAVALTGLHRGIAGAAHTDAPGDLRVDVTVHPDDEPV
                     DGHTRELEREYRAVVEELLELRGDDGRIAAFVRSITEPGVLADTSGYSPDISYEQKVQ
                     LLATLDVTERLELALKLQRERLTELQVRKRIRDDVQQGADKQQREYFLRKQMESIRKE
                     LGEDDASVADEYRSKIEESGMPEAVREQAEKELARLERMGEQSGESSMIRTYLDWLVA
                     VPWDKRSEERLDPTHAREVLDADHAGLEDVKDRIEEHIAVMKLRRERELGPADGDGTD
                     RKRGEGAILTLIGPPGTGKTSIGESIARATGREFVRMSLGGVRDEAEIRGHRRTYIGA
                     LPGRLVRALRDAGTMNPVILLDEVDKVGADWRGDPSAALLEVLDPAQNHSFRDHYLDV
                     ELDLSQVLFIATANVADTIPGPLLDRMEVVRFDGYTTDEKLAIARDYLWPRQRERNGL
                     REDEVDVADDVLRTVIAEYTREAGVRGLERDLGTILRKTATKIASGRVEAPVTVDLDT
                     VRDALGRQRFFQEAAERTAVPGVATGLAVTGAGGDVLFVEATGMKGKGDLVLTGQLGD
                     VMKESARIALSYARGNADRLGIDEDSFDGREFHVHVPAGAIPKDGPSAGITMTTALAS
                     LLTGRPVKHTVGMTGEVTLQGRVLPIGGVKQKVLAAHAAGLTDVILPERNRGDLDDVP
                     EDVREQMTFHLVMTIDEVLDAALEPVRTAVEATH"
     gene            5192..5917
                     /gene="rpiA"
                     /locus_tag="VHF88_03595"
     CDS             5192..5917
                     /gene="rpiA"
                     /locus_tag="VHF88_03595"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX2160884.1"
                     /translation="MTSVRRRDDAAPVTDEPSPARDAASAAALELIEPGMTVGLGSGR
                     AVWRVVERIAERWPEGPPLRAVVASELTHARAREAGIEVVELDGTFELDLAIDGADEL
                     DGDLRLIKGGGGALLREKVIVAAARRFVVVAETGKLVDRLGERFRLPVEVARFAWRDT
                     RRRLAELLPDPELRTAGDSAFLTDEGHHILDCQLPAGSDPDSLAAELRRIPGVLEHGL
                     FIGMAEQAILGTPAGTVERVSAS"
     gene            complement(5914..6633)
                     /gene="cpaB"
                     /locus_tag="VHF88_03600"
     CDS             complement(5914..6633)
                     /gene="cpaB"
                     /locus_tag="VHF88_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX2160885.1"
                     /translation="MSARTRRRRGVVLLVLALACGALAASEVGDRVSEVEARIGAPVP
                     VVVAARDIEPGTELSGGDLRLSYVPQRFAPPDAPTRPAQAVGLHTAGPVAAGSPITAG
                     VVGAAAGADDPGALRRGERAVELAVAGGSSLVETTGPGARVDVLVSTDPGDGPGSSFV
                     ALEDVELLALSPGGSGAPELDADLGAAAPTSAIATLRVTLRQAVYLTAAQSYAREIRL
                     LPRPPGDRRRVGRATVGAQDL"
     gene            complement(6630..7340)
                     /locus_tag="VHF88_03605"
     CDS             complement(6630..7340)
                     /locus_tag="VHF88_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2160886.1"
                     /translation="MAVVIALTGWLAAAALTLAVLRLRRRLALVADAAHELRGPVTAF
                     AYAVASLRREAGGVRRALPFEAELERMRMGLADLDAARVGRRTPLRPRTMALERVVIG
                     AASGWRAAVSGPGRGVTVRWEAGRAAVSADRARLAQAFGNLLANAAEHGSGPIEIHAV
                     RKGPRAVRVEVRDGGARAPVRQGARAGRPIDRERGRGLGIAERAIREAGGTFELERGR
                     AGTVAVVELPIASKETER"
     gene            complement(7343..8047)
                     /locus_tag="VHF88_03610"
     CDS             complement(7343..8047)
                     /locus_tag="VHF88_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2160887.1"
                     /translation="MNQTLLIVEDDESTAEFLTDNLVADGYRVAVASGAAEGVRQIEV
                     RHPALVLLDLKLDDGSGLALLDRVRSADGLTSRIDPELPVIVISGRGSEADRVRSFDR
                     GADDHIQKPLAYGEVLGRIRAVLRRSEGRPQRGVVRYAGLTLDPVTRLVCVNSKPVHL
                     STMEFSLLQRLADEPERVYSKAELLRDVWGYLSPGKTRTVDAHACRLRRKLAADSERR
                     WIVNVRGIGYRLTEPV"
     gene            8195..8656
                     /locus_tag="VHF88_03615"
     CDS             8195..8656
                     /locus_tag="VHF88_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160888.1"
                     /translation="MDSTKRGNYESGADYVLEYGELKFTFNERDFTERVEQAATKLGF
                     VGGPLGEEEREDLVNLAVNGEVTDAHSLLGEHIMDCWGDLHGPSDRSLVHWLRRLVFR
                     GAWLDQRVLEGELEIVFDDETHSFGYAQPERGWELIELSPEPSWKRVAYRR"
     gene            complement(8653..9369)
                     /locus_tag="VHF88_03620"
     CDS             complement(8653..9369)
                     /locus_tag="VHF88_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX2160889.1"
                     /translation="MTQDPRRPTTTDEELLEAESLTILSELDDEARLARIDGELRDGF
                     EALAHVGKAVSFFGSARTLPDAPDYAAARSLARRMGEEGFAIITGGGPGTMEAANRGA
                     RDAGAHSIGLAIELPHEEAPNPHIDIGLTFHYFFVRKVMFVRYSSGFVVFPGGFGTLD
                     ELFEAATLRQTEKIRHFPIVLFGTDYWGGLVDWLRDSALAGGKISPHDVDMLELTDDP
                     DRVLEVMHSIEHRRPRRAAS"
     gene            complement(9392..10591)
                     /locus_tag="VHF88_03625"
     CDS             complement(9392..10591)
                     /locus_tag="VHF88_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX2160890.1"
                     /translation="MSVLQRKELEDSPLADLHAIASELGIEGYRALRRDDLIGSILDV
                     QSGGGPSSKEEEADAPVERPRPRRPRLRGRGRGARTRGESADEPEAEQPEAELDDEPA
                     ATAEVDADGGDSPDVGDLVEAGEDEQRETVTGVLDVLPNGSGFLRVAEGPDVYVSPAQ
                     IRRCELRSGDEVSGPVRPARRNESHPSLVRVESVNGGPAEPPAARPNFDDLTPVHPQE
                     RLAAPAAMSAVPFGKGSRVAIAGPSGAGATRLLREIVAAIRAETSGPSLTVVLAGVRP
                     EEVTEWRRESKTTVVGGSFDGSAEEQAQAAQLAVERAKRVVERGGDAIVAIDSLAAVP
                     ADAARRVFGAGRRVEEGGSLTVIATVGEGGEQLRVASTRVVLAGGGGEPAVVAERSGA
                     QRADLFG"
     gene            complement(10635..11522)
                     /gene="folP"
                     /locus_tag="VHF88_03630"
     CDS             complement(10635..11522)
                     /gene="folP"
                     /locus_tag="VHF88_03630"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2160891.1"
                     /translation="MRLALGDRSLDLPAGAPALMGIVNATPDSFSDEQAPKSLAALVE
                     HGRALAAAGAAIVDVGGESGRSDRPPVSVDEEASRVVPLVERLSADGIAVSVDTWRAE
                     VARAALDAGAILINDVSGLSDPAVAAACAVSGAGLVVTHTRVAPKAKGFPEYSNVVAD
                     TIELLAARAAAANEQGVAQEQLLLDPGLDLAKTPAESVELLRRLPELEHLGRPLLLAI
                     SRKDFVGALTGRRPRERDAGTLGAIEPALDCAAGTVLRVHDVAAAADFIAVRAALRGT
                     AAAPTSPLEDSLRRESAPA"
     gene            complement(11519..13219)
                     /locus_tag="VHF88_03635"
     CDS             complement(11519..13219)
                     /locus_tag="VHF88_03635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX2160892.1"
                     /translation="MIARADALNALERDEFDVVVIGGGITGAGVALDAASRGYSVALV
                     ERHDYAEGTSSRSSKLVHGGLRYLQNFDLGLVREALLERQLLVNLAPHLVRPLPLLIP
                     AFDGRRPDRVLGVGLNMYDVMATERRRRRGGEEPRRRRVDEESWSPDRHRTIDAEETL
                     RVLPALAAREPTAAYLFYDCQTDDVRLTLTILGEAERYGAVCANAVEATGLVERDGRA
                     GGILCRDAIGGGEFELRAANVVNATGVWADRIRPDELHGEAEVPRIRPSRGTHITVSR
                     EQLDVVGGAIVPAGGGRSIFVLPWLGRTLIGTTDNDYESSELDHIPPGGEDIEYLLDA
                     TNEFFATSLTASDLTGAYAGVRPLISTGDPKKSVDISRKAELYETSSGLVTITGGKLT
                     TWRRMAKLAVDRIVEREGREAPCRTAEIPLGMELDPSALPDVEGVDDATRQMLAGRYG
                     HGAIDVMRLAASDPALARRISPELPDIVAEAVCAARQEQARGVADVLLRRTRLGLLDA
                     RNLTEPDAAGPRAVADAMAAELGWDAARVERELSDWRSVASAEGLAPVESVTASTERS
                     "
     gene            complement(13216..13725)
                     /locus_tag="VHF88_03640"
     CDS             complement(13216..13725)
                     /locus_tag="VHF88_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX2160893.1"
                     /translation="MELTDAIRGRRTHKAYAPNPVDRPLLDDLFDLARWAPNHHLTNP
                     WRFRVLGPDSLTRLKAAAGPEAAAKLDRAPTLVCASVKLTGDPVQDEEDLCAAACAVF
                     IVLLAAHDRGLAGYWRTPGVLRTPDGAAAVGMDRDELFVALIHLGYARQEKRPPDRLP
                     PADFVSYLP"
     gene            complement(13868..>14177)
                     /locus_tag="VHF88_03645"
     CDS             complement(13868..>14177)
                     /locus_tag="VHF88_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160894.1"
                     /translation="GLAAARVPLSSLTSDPLAYLRNLQRRDGSFAYSTTSDQTPVWVT
                     AQALAALQRKPFPLKPVKRGAKFATATHPNKPNGPSGPVIAAGLAAAAILAAAVAWKH
                     "
BASE COUNT         1888 a         5224 c         5148 g         1917 t
ORIGIN      
        1 gtcgccgcga acaccgccac ggacgcggtc accgccccac cggccccggc tccggcgcgg
       61 aggagccggc gtcgccgagc cgcgccgcca gacggcccgc ggcgagctcg ccgacgcggc
      121 cgccctcggc gaccgcgcgc acgtcggcgc gaccgtcgca cgcaaccacc tcggtcaccg
      181 cctcgacgac gcgcgagccg tccggtcggc gcgcctggtg gaccaccaac tccaccgctc
      241 gtgcgacctg gtcgcggacg acggcgtgcg gcaagcccac ccccgccatc aacgcgagcg
      301 tctcgagtcg cgcgagcgcg tcgcgcgccg agttggcgtg gacggtcgac agcgagccct
      361 cgtggccggt gttgagcgcc tgcagcaggt cgagcgcctc gccgccgcgg acctcgccga
      421 cgacgatccg gtccgggcgc atccgcagcg cgttgcggac cagcgcccgg atcgtcacct
      481 cgccgcgacc ctcgaggctc ggcggccgcg actcgagtcg cacgacatgc cgctggcgca
      541 gccgcagctc ggccgcgtcc tcgatcgtca cgatccgctc accctcggga atcgccccag
      601 agagcgcgtt gagcgtagtc gtcttcccgg acccggtccc gccgctgacc aggatcgacg
      661 tccgtgccgc tacgcagcgc gcgagcagcg cggcctcgtc gcgcgacagc gtgcctagcc
      721 gcaccaggtc gtcgagcgag agcccctcgc gcctgaagcg gcggatcgtc aggcacgggc
      781 ccgacagcga caatggcggg atcacgacgt tgacgcgcga gccgtcggcc aggcgcgcgt
      841 cgcagagcgg cgaggcctcg tcgacgcgcc gcccgagcgg ggccaggatc cgctcgatcg
      901 cgtggctcag ctcggcctcg gaggcgaacc gcgcgtccgc ccgggcgatc cggccgtgcc
      961 gctcgacgta gacgtcgtcg ggaccgttga ccatcacctc gtcgaccgcc gggtcggcga
     1021 gcagcggctc gagcggcccg agaccggtcg cggccagcag cacacgctcg cgcagctcgg
     1081 cccgttcggc ttcggacagc gcggccgcct cgcgctcgac cagcgccacg atctccgcac
     1141 ggacgtcgcc ggccgactcg ccgcgccgcg cccgctcgat caggcgaccg cgcagcgcac
     1201 cggcaagctc ctcgatcgcc tccactcacc cataggggcg gccggcggcg agcgcgcccc
     1261 cctcgtgtgt ggcgctagct cagctcgtcg cgcgagcgcc gcaggccggc cacggcgcga
     1321 cgccctgctg gcggtagagc cgcagcgcca tccggtcctg atgcgcctcg gaggcagccg
     1381 ccggatcgtc gccgcggccg ccgaccgcct tccaggtcgg gcgcgagaac tggaacttgc
     1441 cgcggtagag gccgttcgac gaaacggcgc gcggatcgcc gccggactcg cactgcgcga
     1501 tctcgcgcag gacgcgcggg agcttgacgt tgctgccccc gccgctggcc ggagcatcct
     1561 tgggcggatg ctggacgtcg gcgctgctga agcgggcgag ccctagcttc ccgcgcgtca
     1621 tcgggccgac gatgccatcc gcgacgagcc cgttgcggcg ctggaagcgc ttgacggcgc
     1681 ggtcggtctg cggcccgaag accccatccg ccgcgatgcc gagcctgcgc tgcaggcgac
     1741 ggaccgcccg cccgcggtcg ccttcgcgca aggtgacctc ggtcgcggct gccgccgcgc
     1801 cggccacgcc gacactgggc tcgtccgcgg acgcctggcc cgcacccggg gcggcgctga
     1861 acagcgcgat cacactcatg gcaacgacgg tctggcggcg caacttcacg tctcctcttt
     1921 ccggcgccta cgaggtgagc tgacgggctc gcgctggaaa gtgcgctacg ccgcacgctg
     1981 gcggcgattc gccccggggg ccagaccgtc aggtcggacc ccagtggttc ccccgttctc
     2041 cgccacctgg gcggcggaga ctcggctatg gtcgattcag agccggacag tcaagcagat
     2101 gcggacgatg accgtgggct cgcgcccccg gaccccgtgg cgcctggccg gctaagggtg
     2161 ggtcaacgac gcttcgcgag ccgcccgggc aggtgcagca ggacgcgcac gccctcgacg
     2221 cccggcgtcg tggccgccgt ccgctcgagc tcgcggatcg cctcggggct gtctacacga
     2281 ccgcgcagga cgaccatccg gcccgcgacc tcgacgtcga cgttgccgcc cgcctcctgc
     2341 agcggcgtgg caagcgcggc gcggaccgcg ccgtcgaggt cggcctcggc ggcggtgggc
     2401 ggctcgggcg tcggcgggct gggcgtcgga ctcgggtccg gaactgtcgg cggaggtggc
     2461 tgcggctcgg gggtcggcgc cggatccggg accggcgtcg ggctcggctg aggctcaggc
     2521 gtcggctcgg gcgccgggat cggatcggga accgggctcg gcggctctgg cgcaggcgcc
     2581 ggaggctgcg gtgggccgga gacgacgctg agctcacgct gcgcctcgcc gcgccggcgg
     2641 gcaagcatcg cacccgcagc gccggccgca cccgctatca ccagcggcgc ggccctgcgg
     2701 acgatctcgc gcgagaaaaa tcccatggcc accacgatac cggcgccgac gcgtctagtg
     2761 agtcgcctcg acggcggtgc ggacaggctc caacgccgcg tccagcacct cgtcgatcgt
     2821 catgaccaga tggaacgtca tctgctcgcg cacgtcctcc ggcacgtcgt cgaggtcgcc
     2881 gcggttgcgc tcgggcagga tcacgtccgt cagcccggcg gcgtgcgcgg cgaggacctt
     2941 ctgctttacc ccgccgatcg gcagcacgcg gccctggagg gtgacctcgc cggtcatccc
     3001 gaccgtgtgc ttgaccggcc gccccgtcag caacgacgcg agcgcggtgg tcatcgtgat
     3061 cccggcgctc ggaccgtcct tggggatcgc ccccgccggc acgtggacgt ggaactcgcg
     3121 tccgtcgaag gagtcctcgt cgatgccgag ccggtcggcg ttgccgcgcg cgtaggagag
     3181 cgcgattcgc gccgactcct tcatcacatc gccgagctgg ccggtcaata cgaggtcgcc
     3241 cttgcccttc atcccggtcg cctcgacgaa cagcacgtca ccgccggcgc cggtcaccgc
     3301 caggccggtc gccacgccgg ggacggcggt ccgctcggcc gcctcctgga agaagcgctg
     3361 ccgtccgagc gcgtcgcgga ccgtgtcgag gtcgaccgtg accggagcct cgaccctgcc
     3421 cgaggcgatc ttcgtcgccg tcttgcgcag gatcgtgccg aggtcgcgct ccaacccccg
     3481 cacgcccgcc tcgcgggtgt actcggcgat caccgtgcgc agcacgtcgt cggcgacgtc
     3541 cacctcgtcc tcgcgcaggc cgttgcgctc gcgctggcgc ggccagaggt agtcgcgggc
     3601 gattgccagc ttctcgtcag tcgtgtagcc gtcgaagcgc acgacctcca tgcggtcgag
     3661 cagcggcccg gggatcgtgt cggcgacgtt ggcggtcgcg atgaacagaa cctgcgagag
     3721 gtcgagctcg acatcgaggt agtggtcgcg gaacgagtgg ttctgcgccg ggtcgagcac
     3781 ctcgagcagg gccgccgacg gatcgccgcg ccagtccgcg ccgaccttgt cgacctcgtc
     3841 gagcaggatc accgggttca tcgtgccggc gtcgcgcagg gcgcggacca accggcccgg
     3901 cagcgcgccg atgtaggtgc ggcgatggcc gcggatctcc gcctcgtcgc gcacgccgcc
     3961 gagcgacatg cggacgaact cgcgcccggt ggcgcgcgcg atcgactcgc cgatcgaggt
     4021 cttgccggtg ccgggcggcc cgatcagcgt caggatcgcg ccctcgccgc gcttgcggtc
     4081 ggtgccgtcg ccgtcggccg ggccgagctc gcgctcgcgg cgcagcttca tcaccgcgat
     4141 gtgctcctcg atgcggtcct tgacgtcctc gaggccggcg tggtcggcgt cgaggacctc
     4201 gcgcgcatgg gtcgggtcga gccgctcctc ggagcgcttg tcccacggca cggcgaccag
     4261 ccagtcgagg taggtgcgga tcatcgagct ctcgcccgac tgctcgccca tccgctccag
     4321 ccgcgcgagc tccttctccg cctgctcgcg caccgcctcg ggcatgccgg actcctcgat
     4381 cttcgagcgg tactcgtcgg cgaccgaggc gtcgtcctcg ccgagctcct tgcggatcga
     4441 ctccatctgc ttgcgcagga agtactcgcg ctgctgcttg tcggcgccct gctggacgtc
     4501 gtcgcggatc cgcttgcgca cctggagctc ggtgagccgc tcgcgctgca gcttcagcgc
     4561 cagctcgagc cgctcggtga cgtcgagcgt cgcgagcagc tggaccttct gctcgtagga
     4621 gatgtcgggc gagtagcccg aggtgtcggc cagtacgccc ggctcggtga tcgagcggac
     4681 gaacgccgcg atgcgcccgt cgtcgccgcg cagctcgagc agctcctcga ccaccgcgcg
     4741 gtactcccgc tcgagctcgc gcgtgtggcc gtcgaccggc tcgtcgtcgg gatggacggt
     4801 gacgtcgacg cgcaggtcgc ccggcgcgtc ggtgtgggcc gcgccggcga tgccgcggtg
     4861 caggccggtc agcgcgacgg cgtggccgcc gccggggagg cggacgtgct cggagacctc
     4921 ggcgacggtg ccgacggagg cgaactcgcc gtcctcgcgc ggcacgagca gcacgcgctc
     4981 ctcgtcaccg acgtcgacga ccagcgtcac cgtcatgttc gggaagacga ccgagtcgtc
     5041 gaccggcacc aacagcagcg tctgcttctc cgtctccatg cgctcagctc ccaggtgggg
     5101 acttacgatt gataagtagt gtagcgaatc gagtcaggcg atcaggggtc gcataagaca
     5161 acctgatacg aacagactta gatcgtagct gatgacttcc gtccgccgca gggacgatgc
     5221 tgcgcccgtg accgatgagc cctcccccgc tcgcgacgcc gcctccgccg cggcgctgga
     5281 gctgatcgag ccgggcatga cggttggcct tggctcgggg cgcgcggtct ggcgagtcgt
     5341 ggagcggatc gccgagcgct ggccggaggg accgccgctg cgcgcggtcg tcgcgtccga
     5401 gctgacccac gcccgtgcgc gcgaggccgg gatcgaggtc gtcgagctcg acgggacctt
     5461 cgagctcgac ctcgcgatcg acggcgccga cgagctcgac ggcgacctgc gcctgatcaa
     5521 gggcggcggc ggcgccctgc tgcgcgagaa ggtcatcgtc gcagccgcgc gccgcttcgt
     5581 cgtcgtggcc gagacgggca agctcgtcga ccgcctcggc gagcgcttcc gcctacccgt
     5641 ggaggtcgcc cgcttcgcct ggcgcgacac gcgccgccga ctcgccgagc tgcttcccga
     5701 tcccgagctg cgcaccgcgg gtgactcggc cttcctcacc gacgagggcc accacatcct
     5761 cgactgtcag ctgcccgccg ggtcggaccc ggacagcctc gccgccgagc ttcgccgaat
     5821 tccgggcgtc ctcgagcacg gcctcttcat cggcatggcc gagcaggcca tcttggggac
     5881 gcccgcagga accgtcgagc gcgtctccgc cagctagagg tcctgcgcgc cgaccgtcgc
     5941 acgtccgacc ctccgccggt cgcccggcgg ccgcggcagc aaacggatct cacgcgcgta
     6001 gctttgggcg gcggtcaggt agaccgcctg acgcagggtg acgcgcagcg tggcgatcgc
     6061 cgaggtagga gcggcggcac cgaggtcggc gtcgagctcg ggcgctcccg acccgcctgg
     6121 gctgagcgcg agcagctcga cgtcctcgag cgcgacgaac gagctgcccg ggccgtcgcc
     6181 cggatcggtc gagacgagga cgtcgactcg cgcgcccggg ccggtcgtct cgaccagcga
     6241 ggagccgccg gcaaccgcga gctcgacggc acgctcgcca cgccgcagag ctcccgggtc
     6301 gtcggctccc gcggccgcac ccacgacacc ggcggtgatc gggctgccgg cggcgaccgg
     6361 accggcggtg tgtagcccta cggcctgcgc ggggcgggtc ggggcgtccg gtggcgcgaa
     6421 acgctggggc acgtagctga gccgcaggtc gccgccgctc agctcggtgc ccggctcgat
     6481 gtcgcgggcc gcgacgacga cgggcaccgg tgctccgatc cgcgcctcga cctcgctcac
     6541 gcggtcgccg acctccgacg ccgccagcgc gccgcaggcc agggcgagca cgagcaggac
     6601 gacgccgcgg cgccggcgcg tccgggcgct caccgctccg tctccttgga ggcgatcggc
     6661 aactcgacca cggcgaccgt gccagcgcga cctcgttcga gctcgaaagt gccgccagcc
     6721 tcccgaatcg cgcgctcggc gattccgagg ccccggcccc gctcgcgatc gatcggccgg
     6781 ccggctcggg cgccctggcg aaccggagcc cgcgccccgc cgtcgcggac ctcgacccgc
     6841 acggctcggg gccccttgcg cacggcgtgg atctcgatcg ggcccgaccc gtgttcggcc
     6901 gcgttcgcga ggaggttgcc gaaggcctgc gccagccgcg cgcggtcggc cgagacagcc
     6961 gcccgccccg cctcccagcg caccgtcacg ccacgtccgg gaccgctgac cgctgcccgc
     7021 cagccggacg cggcgccgat gacgacacgc tcgagcgcca tggtccgcgg tcgcagcggc
     7081 gtgcgccggc cgacgcgcgc ggcatcgaga tcggccagcc ccatgcgcat ccgctccagc
     7141 tcggcctcaa agggcagcgc ccgccgaacg ccgcccgcct cgcggcgcag cgacgccacg
     7201 gcgtaggcga aggcggtcac cgggccgcgc agctcgtgcg ccgcgtcggc gaccagcgcg
     7261 aggcgccgcc gcaggcgcag gacagccaac gtcagcgcgg cggccgcgag ccaaccggtc
     7321 agcgcgatca cgacggccat cgctagaccg gctcggtgag gcggtagccg atcccgcgca
     7381 cgttgacgat ccatcgccgt tcggagtcgg cggcgagctt gcgccgcagg cggcaggcgt
     7441 gagcgtcgac cgttcgtgtc ttgccgggcg acaggtagcc ccagacgtca cgcagcagct
     7501 cggccttcga gtagacgcgc tcgggctcgt cggcaagccg ctggagcagc gagaactcca
     7561 tcgtcgagag gtggaccggc ttgctgttga cgcagaccag ccgcgtgacg ggatcgagcg
     7621 tcagcccggc gtagcgcacg actccgcgct gcggccggcc ctccgaccgc cgcaggaccg
     7681 cgcggatccg cccgagcacc tccccgtagg cgagcggctt ctggatgtgg tcgtcggctc
     7741 cgcggtcgaa gctgcggacg cggtcggcct cgctgccgcg gccgctgatc acgatcaccg
     7801 gcagctccgg gtcgatccgc gaggtcagtc cgtcggcgga gcggacgcgg tcgagcagcg
     7861 caaggccgct gccgtcgtcg agcttgaggt cgagcagcac cagggccgga tggcgcacct
     7921 cgatctggcg cactccctcg gcggcgccgc tcgccacggc gacccggtag ccgtcggcaa
     7981 ccaggttgtc ggtgaggaac tcggcggtgg attcgtcgtc ctcgactatc aagagcgttt
     8041 ggttcatggc cgatgaagtc acggccgcgt gaaactcgcc cccctggtcg gcgcttcagg
     8101 tgcaattcgc gttgcaatcc gtccggccga gaagggaacg ggactaggat gccgacgcgc
     8161 cacacggctg gccccgccag cgcagcgccc tctcatggac agcaccaaac gcggaaacta
     8221 cgagtcgggc gccgactacg tgctcgagta cggcgagctg aagttcacgt tcaacgagcg
     8281 cgacttcacc gagcgcgtcg agcaggccgc caccaagctc ggcttcgtcg gcggcccact
     8341 cggcgaggag gagcgcgagg acctggtcaa cctggccgtc aacggcgagg tcaccgacgc
     8401 gcactcgctg ctcggcgagc acatcatgga ttgctggggc gacctccacg gcccctccga
     8461 ccgctcgctc gtccactggc tgcggcggct ggtgttccgc ggcgcctggc tcgaccagcg
     8521 cgtgctcgag ggcgagctcg agatcgtctt cgacgacgag acccactctt tcggctacgc
     8581 ccagcccgag cgcggttggg agctgatcga gctctctccc gagccgtcct ggaagcgcgt
     8641 ggcttaccgg cgttagctgg cagcgcgccg cggcctgcgg tgctcgatgc tgtgcattac
     8701 ctccaggacc cggtcggggt cgtcggtgag ctcgagcatg tcgacgtcat ggggtgagat
     8761 cttgcctccg gcgagcgcgc tgtcgcgcag ccagtcgacg aggccgcccc agtagtcagt
     8821 cccgaagagc acgatcggga agtggcggat cttctcggtc tgacgcagcg tcgccgcctc
     8881 aaagagctcg tccagggtgc cgaagccgcc gggaaagacg acgaagccgc tcgagtagcg
     8941 gacgaacatc accttgcgga cgaagaagta gtggaaggtg aggccgatgt cgatgtgcgg
     9001 gttcggggcc tcctcgtgcg gcagctcgat cgccagcccg atcgaatggg cgccggcgtc
     9061 gcgcgcaccg cgattcgccg cctccatcgt cccgggcccg ccgcccgtga tgatcgcgaa
     9121 gccctcctcc cccattcgcc gcgccagcga ccgcgcggcg gcgtagtccg gcgcgtcggg
     9181 cagcgtccgc gccgagccga agaacgagac cgccttgccg acgtgcgcga gcgcctcgaa
     9241 gccgtcgcgc agctcgccgt cgatgcgcgc gagccgcgcc tcgtcgtcga gctccgagag
     9301 gatcgtcagc gactcggcct cgagcagctc ctcgtcggtg gtggtggggc ggcgcggatc
     9361 ctgggtcacg ccgacagcgt atgggccagg tctagccgaa caggtcggcg cgctgggcgc
     9421 cggaccgctc tgcgaccacc gcgggctcac cgcctccgcc tgcgagcacg acccgcgtgc
     9481 tagcgacgcg cagctgctcg cccccctcgc cgacggtcgc gatcaccgtc agcgagccgc
     9541 cctcctcgac gcggcggccg gcgccgaata cacgccgagc ggcatcggcg gggacggccg
     9601 cgaggctgtc gatggcgacg atcgcatcgc cgccgcgctc gacgacccgc ttggcgcgct
     9661 cgacggcgag ctgggcggcc tgcgcctgct cctcggcgga gccgtcgaac gagccgccca
     9721 cgaccgtcgt cttgctctca cgccgccact cggtgacctc ctccggcctg acgccggcga
     9781 gcacgacggt cagcgacgga ccgctggtct cggcgcggat cgcagccacg atctcgcgca
     9841 gcaggcgcgt ggcgccggca cccgagggac cggcgatcgc gaccctcgag cccttgccga
     9901 acggcaccgc cgacatcgca gcgggcgccg ccaggcgctc ctgcggatga acaggagtca
     9961 ggtcgtcaaa gttggggcgt gcggcgggcg gctcggccgg accgccgttg acgctctcga
    10021 cccgcaccag cgacggatgg ctctcgttgc ggcgcgccgg tcgcacggga cccgagacct
    10081 cgtcgcccga ccgcagctcg cagcggcgga tctgcgccgg cgagacatag acgtcggggc
    10141 cctcggcgac gcgcaggaac ccgctgccgt tgggcagcac gtcgagcacc ccggtgaccg
    10201 tctcgcgctg ctcgtcctcg ccggcctcga cgagatcgcc gacgtcgggg gaatcgccgc
    10261 cgtcggcatc cacctcggcc gttgcagccg gctcgtcgtc gagctccgcc tcgggctgct
    10321 cggcctccgg ctcgtccgcg ctctcgccgc gcgtgcgggc cccgcgaccg cggccgcgta
    10381 gccggggccg gcgaggacgc gggcgctcga cgggcgcgtc ggcctcctcc tccttcgagg
    10441 acggcccgcc gcccgactgg acgtcgagga tcgagccgat caggtcgtcg cggcgcaggg
    10501 cacggtagcc ctcgatcccc agctccgagg cgatggcatg aaggtcggcg agcgggctgt
    10561 cttccagctc cttgcgctgc agtactgaca ctgaggctgc tcctaaagtc gatttgagcg
    10621 cgggagagta acgactaggc cggcgcggac tcgcggcgga gcgagtcctc gagcggactc
    10681 gttggcgcgg cagcggtgcc gcgcagcgcg gcacggactg cgatgaagtc ggccgcagcg
    10741 gcgacgtcgt ggacgcgcag caccgtcccg gcggcgcagt cgagcgccgg ctcgatcgct
    10801 cccagcgtgc cggcgtcgcg ctcgcgtggc cgacggccgg tcaacgcgcc gacgaagtcc
    10861 ttgcgcgaga tcgcgagcag caacggccgg cccaggtgct ccagctccgg caggcggcgc
    10921 agcagctcga ccgactcggc gggcgtcttg gcgaggtcga ggcccggatc gagcagcagc
    10981 tgctcctgcg ccacgccctg ctcgttcgcc gccgctgccc gcgccgcgag cagctcgatc
    11041 gtgtcggcga cgacattgga gtactccgga aagcccttcg ccttcggcgc gacgcgggtg
    11101 tgcgtcacca cgaggccggc gccgctgacc gcgcaggcgg ccgcgacggc ggggtcgctg
    11161 aggccgctga cgtcgttgat caggatcgca cccgcatcca gcgccgcgcg tgcaacctcc
    11221 gcgcgccagg tgtcgaccga gaccgcgatc ccgtcggcgc tcaggcgctc gaccagcggc
    11281 acgacgcgtg aggcctcctc gtcgaccgac accggcggac gatcgctgcg gccggactcg
    11341 ccgccgacgt cgacgatcgc ggcgcccgct gcggccagcg cacggccatg ctcgacgagc
    11401 gcggccagcg acttcggcgc ctgctcgtcc gagaacgagt cgggcgtcgc gttgactatc
    11461 cccatcagcg ccggcgcgcc cgcgggaagg tcgagcgagc gatcgccgag cgccagcctc
    11521 acgagcgctc cgtcgacgcc gtcacggact ccactggcgc gagaccctcg gcgctcgcca
    11581 ccgagcgcca atcgctcagc tcgcgctcga cgcgggcggc gtcccaaccg agctcggcgg
    11641 ccatcgcgtc ggcaacggcc cgcggcccgg cggcgtcggg ctcagtcagg ttgcgcgcgt
    11701 cgagcagccc gagcctggtg cgacgcagca gcacgtcggc gactccacgc gcctgctcct
    11761 ggcgcgccgc gcagacggcc tcggccacga tgtccggcag ctccggcgat atccggcggg
    11821 cgagcgccgg atccgacgcc gcgagccgca tcacgtcgat cgcgccgtgg ccgtagcggc
    11881 cggccagcat ctgacgcgtc gcgtcgtcga ccccctccac gtcgggcaag gcgctcgggt
    11941 cgagctccat cccgagtggg atctcagccg tccggcaagg cgcctcgcga ccctcgcgct
    12001 cgacgatccg atcgaccgcc agcttcgcca tccggcgcca cgtcgtcagc ttgccgccgg
    12061 tgatcgtcac gagccccgag ctcgtctcgt agagctcggc cttgcgcgag atgtcgaccg
    12121 acttcttcgg gtcgccggtc gagatcagcg ggcggacgcc ggcgtaggcg ccggtcaggt
    12181 ccgacgcggt gagcgacgtc gcgaagaact cgttcgtcgc gtcgagcagg tactcgatgt
    12241 cctcgccacc cggcgggatg tggtcgagct cgctgctctc gtagtcgttg tcggtcgtgc
    12301 cgatcaacgt ccgcccgagc cacggcagca cgaagatcga gcgcccgccg cccgccggca
    12361 cgatcgcccc cccgaccacg tcgagctgct cgcgcgagac ggtgatgtgg gtaccgcggc
    12421 tcggacggat gcgcggcacc tccgcctcgc cgtggagctc gtcgggacgg atccggtcag
    12481 cccagacgcc cgtggcgttg acgacgttgg cggcgcgcag ctcgaactcg ccgccgccga
    12541 tcgcgtcgcg gcagaggatg ccgccggccc ggccgtcgcg ctcgactagg cccgtcgcct
    12601 cgaccgcgtt cgcgcagacc gcgccgtagc gctccgcctc gccgagaatc gtcagcgtca
    12661 gccggacgtc gtcggtctgg cagtcgtaga acaggtaggc ggccgtcggc tcgcgcgcgg
    12721 cgagcgctgg cagcactcgc agcgtctcct cggcgtcgat ggtgcggtgg cgatccgggc
    12781 tccacgactc ctcgtcgacc cggcgccggc gcggctcctc gccgccgcga cgacggcggc
    12841 gctcggtcgc catcacgtcg tacatgttga gcccgacgcc gagcacgcga tcgggccgcc
    12901 ggccgtcaaa cgccgggatc agcagcggca gcggacggac caggtgcggc gccaggttca
    12961 ccagcagctg gcgctcgagc agcgcctcgc ggacgagccc gaggtcgaaa ttctgcaggt
    13021 agcgcaaccc gccgtggacc agcttcgacg agcggctcga cgtcccctcg gcgtagtcgt
    13081 gacgctcgac cagcgccacg ctgtagccgc gcgacgcggc gtccagcgcg acgccggcgc
    13141 cggtgatccc gccgccgatc acgacgacgt cgaactcgtc gcgctcgagc gcattgaggg
    13201 cgtcggcgcg ggcgatcaag gcaggtagct cacgaagtcc gcgggtggca ggcggtccgg
    13261 cgggcgcttc tcctggcgcg cgtagccgag atggatcagc gcgacgaaca gctcgtcgcg
    13321 atccatgccg accgctgccg ccccgtccgg cgtgcgcagg acgcccggcg tgcgccagta
    13381 gccggcgagg ccgcggtcgt gggcggcgag caggacgatg aagaccgcgc aggcggcggc
    13441 gcagaggtcc tcctcgtcct ggaccgggtc gcccgttagc ttgaccgagg cgcagacgag
    13501 cgtgggcgcg cggtcgagct tggcggcggc ctcggggccg gcggccgcct tcaggcgggt
    13561 gagcgagtcg gggccgagga cgcggaagcg ccaggggttg gtgaggtggt ggttgggtgc
    13621 ccagcgggcg aggtcgaaga ggtcgtcgag gagggggcgg tcgacggggt tgggggcgta
    13681 ggccttgtgg gtgcggcggc cgcggatggc gtcggttagc tccacgaggg gcgacggtat
    13741 cggagtgggt ggtggtactc gctagggatc ggcgttgctg gccgcgggtc ctgtctccga
    13801 ttttggtttg cgtgctcgca ggctgcgcgc gcaaacgcaa aatcgtcgcc acccgcggca
    13861 agcggtgtta gtgcttccag gcaacagctg ctgcaaggat cgcggcggct gcgagaccgg
    13921 cggcgatgac tggccctgaa gggccgttgg gcttgttggg atgagtggcg gtggcgaact
    13981 ttgcgccgcg cttgacgggt ttgagcggga agggcttgcg ttggagggcg gctagggctt
    14041 gggcggtgac ccagacgggg gtttggtcgc tggtggtgga gtaggcgaag ctgccgtcgc
    14101 ggcgttggag gttgcggagg taggcgagtg ggtctgaggt gagcgagctc agtgggacgc
    14161 gggcggcggc gaggccc
//
LOCUS       DATABV010000088         9884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2276468, whole genome shotgun sequence.
ACCESSION   DATABV010000088 DATABV010000000
VERSION     DATABV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276468"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1482
                     /locus_tag="VHF88_03650"
     CDS             235..1482
                     /locus_tag="VHF88_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160895.1"
                     /translation="MNRSRRPRDRLAQVRAPRRLLSLSSDDRLVERVIDGDDTAFELL
                     YARHVADALAFAAGVLGSQEEAEEAVRHSFAAAHAYLAGGGRRTAFEPWLYAILENHC
                     LSVLQQRAPEPDGPERLATVVDLDEWRRRRRRLIGVGLPFAPSAAFHDSVMSACGIGA
                     GTATVAAPLLGGTMAKVAVVAVLAGGAGIAGTTASGGDPAGSGTGSSGPPPVADIAEQ
                     QRPRGDDSGLHPELPVPLAALDERAAARGPSASFPDRLHRNRDRPVPPADTALRPIAN
                     APNDAPSSAVPGGYVERGDDAGVPPATGTPNVPLDVPALSAPDGPARLPLTEGVKERV
                     PRVVERVKARLPAPRRPPKVTSPEAVKPPPVDLPTVAKPPRLELPDVGKLPKVRDDGG
                     VIPVKPSAAVGTLLTGGADAPLR"
     gene            1479..2126
                     /locus_tag="VHF88_03655"
     CDS             1479..2126
                     /locus_tag="VHF88_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2160896.1"
                     /translation="MSADEPALEEIAVAAEPTAWRDAGFRIRGDATAAVDVGTVAIDL
                     VGAGAGKRVVRCGLSGIGDANLDGLPIARSEPRARVEPAPAHPNGVVRIDHLVAFTPD
                     LDRTVAAGTALGLDLRRIRDEPAPAGSPRQAFFRIGEPILEVAQAPPGSPLAGHGPAR
                     FDGLAFVVEDLDATVASLGELCGKPRDAVQPGRRIATARKEAKLGLPVAFMTPRP"
     gene            2149..2451
                     /locus_tag="VHF88_03660"
     CDS             2149..2451
                     /locus_tag="VHF88_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160897.1"
                     /translation="MDPAALRNRLLVATGMWRDSTEEPLPKLPPGDPLEQIEAFELRV
                     VEVMFSEATPETARRVADRTWDLVHDRPDDDPVKRRVVEGHEQLAKLSAGGLTAPE"
     gene            2457..5006
                     /locus_tag="VHF88_03665"
     CDS             2457..5006
                     /locus_tag="VHF88_03665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX2160898.1"
                     /translation="MADSPELFSRALMARSLAYLFTGGALVGCLTLAFPHDAAVKDGP
                     LYALAALAVAIGLVVLWRAESMPSWGMHLALATGSMILAAGNYFVGPSGLYLIIFTWT
                     GLYAFYFYRPPVALAHLGVIALSYLVVLIVQDPPSPVVRWVLAVGTPGLAGLMVSALL
                     DRLRRETAQATRQEESLRESEARTRAIVDSAPEPFLGLSEQGTVISWNPAAERTFGWT
                     AEEAVGQPIRDLIVAPENHDDHRQRREEEFARPDGEVRPSRREVELVRRDGARFLAEI
                     TYSRVRAGDRPMLACFIRDISDRKEREREREQLYREQAAREEAEQMAGIVHGLQMLLD
                     VALAHNRLDEMLAALLPRVCEVVNADGAAVLLAEEDGSLTLRASMFRDVEKEPLRIEL
                     GEGIAGRVAQSRQPLLVQDPDPSEVADPALYGLHSILGVPLMAGGEVAGVLKVGVPAP
                     RRFSEDDLMVLGLAADRVALAIDHLRIYEREHRIAEMLQRSLLPDRLPSLPGLEVAAR
                     YEPAASESEVGGDWYDVIAMPGGRVGLVMGDVAGKGLAAASMVGRLRSAMRAYALEGH
                     PPAEVVDRLNQLVWSEIEDSHMTTMVYVVFDPLAGTITWVNAGHLPPLAVTSDGIARF
                     LSGPSSVPLGVMSYPAYEEAETEMPLGGTVLLYTDGLVERPGELLDDGLDRLVAAVRG
                     ERLGPQELCDLVLDRLMPTAGAADDVALLALCSPPLSDAFQLELGSEPGELASLRALL
                     RRWLRHGDGDETEIAEILTATGEAAANAIEHGGSIAGGPPFEVSGRLAGDEVEIVVRD
                     SGGWRERQRSEGGRGLVLMRALMDSVDVERGPDGTTVTMRRTIGTRVPTEA"
     gene            5049..6428
                     /locus_tag="VHF88_03670"
     CDS             5049..6428
                     /locus_tag="VHF88_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2160899.1"
                     /translation="MPSTRRRVCVIGAGSSGIASCQVLHARGIDFDCVEKGSGVGGNW
                     RYGNDNGMSSAYESLFINTSREIMEYATYPMPKSYPDYPHHTQIAAYFDDYVDHFGFR
                     HKIRFRTEVTRVQRAPDGGWDVTARPVDGGDEQTERYDDVMVANGHHWDPRWPEPPFP
                     GELDDSVEVTHAHYYRSSEGYEGKNVLVLGIGNSACDIAVETSRVSKMTFLAMRRGAW
                     IIPKYVGSVPADEILPHWAWRLPLPIVRMLSLIGVRRAAGQLTAFGMPKPDHKLLEAH
                     PTVSSDLLPRIGHGRITPKPNIARLEGDSVRFEDGSVERIDKIVYCTGYKISFPFFDP
                     DLLDPSGDNRIELYRRVVHPDLPGLYFIGLVQPLGAIMPIAELQSEWIADVIEGRVPL
                     PGRAEMVQEIAVEQTAMRRRYVASKRHTIQVDYWPYLRQLREERKRRAAAPQTGAPTG
                     RVANEEIAA"
     gene            6425..7945
                     /locus_tag="VHF88_03675"
     CDS             6425..7945
                     /locus_tag="VHF88_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2160900.1"
                     /translation="MTATVPETATATIEVENPATGQIAGSVPRMLPEDVAPMVERARS
                     IQPAWWGLGFDGRAAVLRRAQKWTLDNADRIVDTIVAETGKTRDDAQLAEIGYAAGAF
                     AFWAKNAEKFLRERKVRTSSPTLLGRKALVRYRPRGVVGVIGPWNYPLVNSFGDCIPA
                     LAAGNAVILKPSEVTPLTSLLMAECLAESGAPDGVFQVLTGLGDTGAELIDHVDFIMF
                     TGSTATGRKVAERAARTLTPVGLELGGKDPMIVLADANLERAANAAAYYSMQNGGQTC
                     ISVERVYVEEPIYEDFAKLVTDKVAKLRQGAPQQAGSVDVGAVTFPRQAEIIEAHVED
                     ARAKGAKVAVGGKRGDGPGNFFEPTVLLDVDHSMDCMTDETFGPTLPIMRVRDAEEAL
                     RLANDSSYGLQASVWTRDTARGEQLAARLEAGAANVNEHQLNYFALELPMGGWKDSGL
                     GTRHGAEGIRKYARQQASVINRFGMKRDIFMFPYSKRSAGLIGRFLKLVYGRGSRS"
     gene            7994..8230
                     /locus_tag="VHF88_03680"
     CDS             7994..8230
                     /locus_tag="VHF88_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX2160901.1"
                     /translation="MSDDLSPENRIKIVVDRGLCIGSGDCVDSAPNVFELDAEDKARV
                     IDPDGDPTDMVVDAAMNCPVTAIFVEGDDGPIYP"
     gene            complement(8234..8521)
                     /locus_tag="VHF88_03685"
     CDS             complement(8234..8521)
                     /locus_tag="VHF88_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160902.1"
                     /translation="MTDREIDVPDDPTGIDQPHDVLAAEEFAMPARSAGDIPPDPTGI
                     QEPHDVLAAEEFAMPGAGVDHEAGGSVDPRSLIPGALLLLVVLLLLRRRRG"
     gene            8578..9369
                     /locus_tag="VHF88_03690"
     CDS             8578..9369
                     /locus_tag="VHF88_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160903.1"
                     /translation="MRRAGSTAVLAIAVVAVLAGCGGGEGSGDATAGERPRPTEVVLT
                     PDSVDPAAARRAAKVDSNGNRRGTPVELGGVGADPPAPGAASPADGISPGAPSDAEVR
                     AELRQARAELSKFKRFLGTTAFLQTGPRARVLPDGTAVAPDDAPEPVKRVIQAGNAIA
                     KFPYKWGGGHGAWRDNGYDCSGSVSFALAGAGLLGSPLTSGGFMDWGASGPGEWITIY
                     TNPGHIFMIVAGLRFDTSGQGRAGTRWQEEPRSTDGFVVRSVPGL"
     gene            9433..>9884
                     /locus_tag="VHF88_03695"
     CDS             9433..>9884
                     /locus_tag="VHF88_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160904.1"
                     /translation="MHDEPTRQMRTGGGDRRGPDDHSVYMRRRLAAAAAAAIALIVVI
                     AVVAGGGGDDSESASPLDVSIPTTPDNLDTTVGDEDEDETDTTTAPSPTPSTGGTAGG
                     TTGGGTVAPDAAGGAATGLRGYRAAAGVGRDGPAACGPTSRAAGRRRRT"
BASE COUNT         1376 a         3602 c         3547 g         1359 t
ORIGIN      
        1 cgggcggtcg gccaccggac ggccgcccga cgaccttccg agcgccaggc cgcctcagac
       61 gtcgcgtccg tccgcgccct cgtctgcttc gccggcgccg agcagtcgcg agaccgcgcc
      121 cgcaggcgcg ggcaacgcgc ctgcggacaa gcggcctgtc ccgcctggct gagctgcgaa
      181 gcgccgattg gccgagtttc ggggctcccg ctgcgcggta cctcagtagg accggtgaac
      241 cgctcgagac gccccagaga tcgtctggcg caggtccgcg cgccgcgacg gctgctgtcg
      301 ctcagctccg acgaccggct cgtcgagcgg gtgatcgacg gcgacgacac ggcgttcgag
      361 ctgctctatg cccgtcacgt cgctgatgcg ctcgccttcg ccgcgggcgt gctcggttcg
      421 caggaggagg ctgaggaggc ggtgcgtcat tcgttcgcgg cggcgcacgc ctatctggcg
      481 ggcggcgggc gcaggaccgc cttcgagccg tggctctacg cgatcctcga gaaccactgc
      541 ctgtcggtgc tgcagcagcg cgcgcccgag cccgacggtc cggagcggct cgcgacggtg
      601 gtcgacctcg acgagtggcg ccggcgtcgt cgccggctga tcggcgtcgg tctgccgttt
      661 gcgccgagcg cggcgtttca cgacagtgtg atgagcgcct gcgggatcgg tgctggaacg
      721 gcgaccgtgg ccgcgccgct gctcggcggc accatggcga aggtcgcggt cgtcgccgtc
      781 ctcgcaggcg gcgccggtat cgcgggcacc accgcgtccg gaggcgaccc agccggctcc
      841 ggcaccgggt cgtccggtcc gccgcctgtg gccgacatcg ccgagcagca gcgaccgcgg
      901 ggcgatgatt ccgggctgca cccggaactg ccggtgccgc tggccgcact cgacgagcgg
      961 gccgctgcgc gaggcccgtc cgcaagcttc cccgaccggt tgcaccgcaa tcgcgaccga
     1021 cccgtgcctc cggcggacac agcgctccga ccgatcgcga acgccccgaa cgacgccccg
     1081 agcagcgccg ttcccggtgg ctacgtggag cgcggcgacg acgccggcgt cccaccggcc
     1141 acgggcactc cgaatgtccc gctcgatgtc cccgcgctct cggcgcccga cgggccggcg
     1201 cggctgccgc tgacagaggg tgtgaaggag cgggtgccgc gtgtggtgga gcgagtgaag
     1261 gctcgccttc cagctccgcg acggcctccc aaggtcactt ctccggaggc cgtcaagccg
     1321 ccccccgtcg atctgcccac ggtcgccaag ccgccgcgcc tcgagctgcc cgacgtcggc
     1381 aagctgccca aggtccgcga tgatggcggg gtgatccccg tcaagccctc ggccgccgtc
     1441 ggcacactgc tgacgggcgg ggccgacgca ccgctccggt gagcgcggac gaaccggcgc
     1501 tcgaggagat cgccgtcgcc gccgagccca cggcctggcg cgacgccggc ttccgcatcc
     1561 gcggcgacgc gacggccgca gtcgacgtcg ggaccgttgc gatcgacctg gtgggtgccg
     1621 gcgccggcaa gcgcgtggtc cgctgcggct tgagcggtat cggcgacgcg aacctcgacg
     1681 gcctgccgat cgcgcgctca gagccgcggg ctcgcgtcga gccggctcca gctcacccca
     1741 acggcgtcgt gcgaatcgac catctcgtgg ctttcacacc cgacctcgac cggaccgtgg
     1801 ctgccggcac ggcgctcggt ctcgacctgc gccgcatccg cgacgagccg gcgccggccg
     1861 gctccccgcg ccaggcgttc tttcggatcg gcgagccgat tctcgaggtc gcccaggcgc
     1921 cgcccggctc gccgctcgcc ggccacggtc cggcccgctt cgacgggctc gcgttcgtcg
     1981 tcgaggacct cgacgcgacg gtggccagtc tcggcgagct ctgtggcaag ccgcgcgacg
     2041 ccgtgcagcc gggccgcagg atcgccacgg cgcgcaagga ggccaagctc gggctgccgg
     2101 tcgcgttcat gacgccgcgg ccttagaggg ccgatagcct gcggggacat ggacccagcc
     2161 gccctgcgca accgcctgct cgtcgccacc gggatgtggc gcgattcgac cgaggagccg
     2221 ctcccgaagc tgcctccggg cgatccgctc gagcagatcg aggccttcga gctgcgggtc
     2281 gtcgaggtca tgttcagcga ggcgaccccc gagacggcga ggcgcgtcgc cgacaggacg
     2341 tgggatctcg tgcacgaccg cccagacgac gacccggtca agcggcgggt ggtcgagggc
     2401 cacgagcagc tggccaagct ctccgccggg ggcctcacgg ccccggagta gcgctcgtgg
     2461 cggactcgcc ggagctgttc agccgcgcct tgatggcgcg ctcgctcgcg tacctgttca
     2521 ccggcggagc gctggtcggc tgtctgacgc tcgcattccc gcatgacgcc gccgtgaagg
     2581 atggcccgct ctacgccctc gccgcgctgg cggtggcgat cggcctggtg gtgctctggc
     2641 gcgcggagtc gatgccgagc tggggcatgc acctcgcgct cgcgaccggc agcatgatcc
     2701 tcgccgccgg caactacttc gtcgggccga gcgggctcta cctgatcatc ttcacctgga
     2761 ccggccttta cgccttctac ttctaccggc cgccggtcgc gctggcccac ctcggtgtga
     2821 ttgcgctgtc gtacctggtg gtgctgatcg tccaggatcc gccgagcccg gtcgtgcgct
     2881 gggtgctggc ggtcgggacg ccgggactcg ccggactgat ggtctcggcg ctgctcgacc
     2941 gattgcggcg cgagaccgcc caggccaccc gccaagagga gagcctgcgt gagagcgagg
     3001 cgcgcacgcg ggcgatcgtc gacagcgcgc cggagccgtt cctcggtctc tccgagcagg
     3061 gcacggtgat ctcctggaac ccggctgccg agcgcacctt cggctggacc gccgaggagg
     3121 ctgtcgggca gccgatccgc gacctgatcg tcgcacccga gaaccacgat gaccaccgcc
     3181 agcggcgcga ggaggagttc gcgcgtccgg acggcgaggt gcgcccgtcg cggcgcgagg
     3241 tcgagctggt gcgacgcgac ggcgcccgct tcctcgccga gatcacctac tcacgcgtgc
     3301 gcgccggcga ccgcccgatg ctcgcgtgct tcatccgcga catcagcgac cgcaaggagc
     3361 gcgagcgcga gcgcgagcag ctgtaccgcg agcaggcggc gcgcgaggag gccgagcaga
     3421 tggcgggaat cgtccacggc ctgcagatgc tgctcgacgt cgcgctcgcc cacaaccgcc
     3481 tcgacgagat gctcgcggcg ctgctgccgc gcgtctgcga ggtcgtcaac gccgacgggg
     3541 cggcggtgct gctcgccgag gaggacgggt cgctgacgct gcgcgcctcg atgttccgcg
     3601 acgtcgagaa ggagccgctg cgaatcgagc tcggcgaggg catcgccggt cgcgtcgcgc
     3661 agagcaggca accgctgctc gtccaggacc ccgacccgtc cgaggtcgcc gacccggcgc
     3721 tgtacgggct tcactcgatc ctcggcgtgc cgctgatggc cggcggcgag gtcgccggcg
     3781 tgctcaaggt cggcgtcccg gcgccgcgcc gcttctccga ggacgacctg atggtgctcg
     3841 ggctggccgc ggaccgggtg gcgctggcga tcgaccatct gcgcatctac gagcgcgagc
     3901 accggatcgc cgagatgctc cagcgcagcc tgctgccgga ccgccttccg agcctgcccg
     3961 gcctagaggt cgcggcccgc tacgagccgg ccgccagcga gtccgaggtc ggcggcgact
     4021 ggtacgacgt gatcgcgatg cccggggggc gggtcggcct ggtgatgggc gacgtggccg
     4081 gcaaggggct ggccgcggct tcgatggtcg gccgcctgcg cagcgcgatg cgcgcctatg
     4141 cgctcgaggg ccatccgccc gcggaggtgg tcgaccggct caaccagctc gtctggtcgg
     4201 agatcgagga cagccacatg acgacgatgg tctacgtcgt cttcgacccg ctcgcaggga
     4261 cgatcacctg ggtcaacgcc ggccacctgc cgccgctggc ggtaaccagc gacggcatcg
     4321 cgcgcttcct cagcggcccg agctcggtcc cgctcggcgt catgtcgtat cccgcctacg
     4381 aggaggcgga gaccgagatg ccactcggcg gcaccgtgct gctctacaca gatgggctcg
     4441 tcgagcggcc cggcgagctg ctcgacgacg gcctcgaccg gctcgtagcg gccgttcgcg
     4501 gcgagcggct cggtccgcag gagctctgcg acctcgtcct cgaccggctc atgccgacgg
     4561 ccggcgccgc cgacgacgtc gcgctgctcg cgctctgcag ccccccgttg tccgacgctt
     4621 tccagctcga gctggggtcc gagccgggcg agctcgcctc gctgcgtgcg ctgctgcgcc
     4681 gctggctgcg ccacggcgac ggcgacgaga ccgagatcgc cgagatcctg accgccaccg
     4741 gcgaggcggc tgccaatgcg atcgagcacg gcggctcgat cgccggcggg ccaccgttcg
     4801 aggtcagcgg acgcctcgcc ggcgacgagg tcgagatcgt cgtgcgcgac tcgggcggct
     4861 ggcgcgagcg tcagcgctcg gagggcggcc gcgggctggt gctgatgcgc gccctgatgg
     4921 actccgtcga cgtcgagcgc ggcccggatg gcacgaccgt gacgatgcgg cggacgatcg
     4981 gcacccgcgt gccgaccgag gcatgacttc gtctggccag ccagccagtc tccgctaggc
     5041 tgcgcctcat gccttccacg cgccgacgtg tctgtgtcat cggcgccggc agctcgggaa
     5101 tcgccagctg ccaggtgctg cacgcgcgcg ggattgactt cgactgcgtc gagaagggct
     5161 caggggtcgg cggcaactgg cgctacggca acgacaacgg aatgtcgtcc gcatacgagt
     5221 cgctgttcat caacacctcg cgcgagatca tggagtacgc gacctacccg atgccgaagt
     5281 cctatccgga ctacccgcac cacacccaga tcgcagccta cttcgacgac tacgtcgacc
     5341 acttcggctt ccgccacaag atccgcttcc gcaccgaggt gacgcgcgtg cagcgggcgc
     5401 cggacggcgg ctgggacgtt accgcgcggc cggtggacgg aggagacgag cagacggagc
     5461 gctacgacga cgtgatggtc gccaacggcc accactggga cccgcgctgg cccgagccgc
     5521 cgttccccgg cgagctcgac gactccgtcg aggtgaccca cgcgcactac taccgcagca
     5581 gcgagggcta cgagggcaag aacgtgctgg tgctggggat cggcaactcg gcctgcgaca
     5641 tcgccgtcga gacctcgcgc gtctcgaaga tgacctttct tgcgatgcgt cgcggcgcct
     5701 ggatcatccc caagtacgtc gggtccgtcc cggccgacga gatcctgccg cattgggcgt
     5761 ggcggctgcc gttgccgatc gtccggatgc tgtcgctgat cggcgtgcga cgtgccgccg
     5821 gccagctgac ggcgttcggg atgccgaagc ccgaccacaa gctgctcgag gcgcatccga
     5881 cggtctcctc cgacctgctg ccgcgcatcg gccacgggcg aataaccccg aagccgaaca
     5941 tcgcgcgcct cgagggcgac tcggtccgct tcgaggacgg ttcggtcgag cggatcgaca
     6001 agatcgtcta ctgcaccggc tacaagatca gcttcccgtt cttcgacccc gacctgctcg
     6061 acccgtcggg cgacaaccgg atcgagctct accggcgcgt cgtgcacccc gacctgccgg
     6121 gcctttactt catcggcctg gtccagccgc tcggggcgat catgccgatc gccgagctgc
     6181 agtcggagtg gatcgccgac gtgatcgagg gccgcgtgcc gctgcccggg cgggccgaga
     6241 tggtccagga gatcgcggtc gagcagacgg cgatgcgcag gcgctacgtc gcctcgaagc
     6301 gccacacgat ccaagtcgac tactggccgt acctgcgcca gctgcgcgag gagcgcaagc
     6361 ggcgggcggc ggcgccacag accggggcgc ccaccgggcg cgtcgccaac gaggagattg
     6421 ccgcatgacc gccaccgtgc ccgagacagc gaccgcaacg atcgaggtcg agaaccctgc
     6481 caccggccag atcgccggca gcgtgccgcg gatgctgccg gaggacgtcg cgccgatggt
     6541 cgagcgtgcg cgctcgatcc agccggcgtg gtgggggctt ggcttcgacg ggcgcgcggc
     6601 ggtgctcagg cgcgcgcaga agtggacgct cgacaacgcc gaccggatcg tcgacacgat
     6661 cgtcgccgag acgggcaaga cgcgcgacga cgcccagctc gcggagatcg gctacgccgc
     6721 cggggcgttc gccttctggg ccaagaacgc cgagaagttc ctgcgcgagc gcaaggtccg
     6781 caccagctcg ccgacgctgc tcggccgcaa ggccctggtc cgctaccggc cacgtggcgt
     6841 ggtcggggtc atcgggccgt ggaactaccc gctcgtcaac tccttcggcg actgcatacc
     6901 cgcgctcgcc gccggcaacg cggtcatcct caagccgtcg gaggtcacgc cgttgacctc
     6961 gctgctgatg gccgagtgcc tcgccgagag tggcgcgccc gacggcgtct tccaggtcct
     7021 gaccggcctc ggtgacacgg gcgccgagct gatcgaccac gtcgacttca tcatgttcac
     7081 cggctcgacc gcgaccgggc gcaaggtcgc cgagcgggcg gcgcggacgc tgacgccggt
     7141 cgggctcgag ctcggcggca aggacccgat gatcgtgctc gccgacgcaa acctcgagcg
     7201 cgccgcgaac gccgcggcct actactcgat gcagaacggc ggccagacct gcatctcggt
     7261 cgagcgcgtg tacgtcgagg agccgatcta cgaggacttc gccaagctcg tgacagacaa
     7321 ggtcgccaag ctgcgccagg gcgcgccgca gcaggcgggc tcggtcgacg tcggcgccgt
     7381 cacgttcccg cgccaggccg agatcatcga ggcgcacgtc gaggacgcgc gcgcgaaggg
     7441 ggcgaaggtc gccgtcggcg gcaagcgcgg cgacggtccc ggcaacttct tcgagccgac
     7501 ggtgctgctc gacgtcgacc actcgatgga ctgcatgacc gacgagacct tcggcccgac
     7561 gctgccgatc atgcgggtgc gtgacgccga ggaggcgctg cggctcgcca acgactcgtc
     7621 ctacgggctc caggcttcgg tctggacgcg ggacaccgct cgcggcgagc agctcgcggc
     7681 gcgcctcgag gcgggggcag cgaacgtcaa cgagcaccag ctcaactact tcgcgctcga
     7741 gctgccgatg ggcggttgga aggactccgg tctcggcacc cgccacggcg ccgaggggat
     7801 ccgcaagtac gcccgccagc aggcctccgt aatcaaccgc ttcggcatga agcgcgacat
     7861 cttcatgttc ccgtactcga agcgctccgc cggcctgatc ggccgcttcc tcaagctcgt
     7921 ctacggccgc ggcagccggt cgtagacggc tgcgagccgg ccgcagcgca gctcgccggt
     7981 aagctgccgc cggatgtcag acgatctctc gcccgagaac cgcatcaaga tcgtcgtcga
     8041 ccgcgggctc tgcatcggct ccggcgactg cgtcgacagc gcgccgaacg tctttgagct
     8101 cgacgccgag gacaaggcgc gcgtgatcga tcccgacggc gatccgaccg acatggtcgt
     8161 cgacgcggcg atgaactgcc cggtcaccgc catcttcgtc gagggcgacg acggccccat
     8221 ctacccgtag tcgttagccg cgccggcgcc tgagcagcaa caggaccacc agcagcagca
     8281 gcgcgccggg gatcaggctg cgcgggtcga ccgacccgcc ggcctcgtgg tcgacgccgg
     8341 cgccgggcat cgcgaactcc tccgcggcga gcacgtcgtg cggctcttgg atcccggtcg
     8401 gatcgggcgg gatgtcgcca gccgagcggg ccggcatcgc gaactcctcg gctgccagca
     8461 cgtcgtgcgg ctggtcgatc ccggtcgggt cgtcgggcac gtcgatctcg cggtcggtca
     8521 tgtcgacgat cctcgcagac ggcgcgagcg gcgcgcatcg acgctagctt gttggggatg
     8581 cggcgcgcag gctcgacagc tgtcctggcg atcgcggtcg tggccgtgct cgcgggctgc
     8641 ggcggcggcg agggatcggg agacgcgacg gccggcgaac gaccccggcc cacggaggtc
     8701 gtgctgacgc ccgactcggt cgatcccgcg gcggcccgcc gtgcggcgaa ggtcgattcg
     8761 aacggtaatc gtcgcggcac ccccgtcgag ctgggcgggg tcggcgcgga cccgccggcg
     8821 cccggcgccg cctcgccggc cgacggcatc agccccggcg ccccgagtga cgccgaggtg
     8881 cgggcggagc tgcgccaggc gcgcgccgag ttgtcgaagt tcaagcgctt cctcggcacg
     8941 accgccttcc tgcagaccgg tccgcgggcg cgcgtgctgc ccgacgggac ggcggtcgcg
     9001 cccgatgacg ctccggagcc ggtcaagcgc gtgatccagg ccggcaacgc gatcgccaag
     9061 ttcccgtaca agtggggcgg cggccacggt gcctggcgcg acaacggcta cgactgctcc
     9121 ggctcggtct ccttcgcgct cgccggcgcc ggcctgctcg gctcgccgct gacgtccggc
     9181 ggcttcatgg attggggcgc gtccgggccg ggcgagtgga tcacgatcta caccaacccc
     9241 ggccacatct tcatgatcgt ggccggcctg cgcttcgaca cgagcgggca ggggcgcgcc
     9301 ggcacgcgct ggcaggagga gccgcgctcg accgacggct tcgtcgtacg cagcgtcccc
     9361 ggcctctagc ggcggggcgc aggcgcgcgc gggccggttc tcgccgccgc ctcaactgct
     9421 actttcgcgc aggtgcacga cgagccgaca cgccagatgc gaacgggcgg cggcgatcgc
     9481 cgcggtcccg acgaccactc cgtctacatg cgccgccgtc tcgcagcggc ggccgcggcg
     9541 gcgatcgcgc tgatcgtcgt catagcggtg gttgccggcg gcgggggaga cgactcggag
     9601 tccgcctcgc cgctcgacgt gagcattccg acgacgcccg acaacctcga cacgaccgtc
     9661 ggcgacgagg acgaggacga gaccgacacc acgacagccc cgagtccgac gccgtcgacc
     9721 ggcggcacgg ctggcgggac cacaggcggc gggaccgtcg cgcccgacgc cgccggcggt
     9781 gccgccaccg ggctccgggg ctaccgtgcc gccgccggcg tcgggcgcga cggtcccgcc
     9841 gcctgtggtc ccaccagccg tgccgccggt cgacggcgtc ggac
//
LOCUS       DATABV010000089         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_348485, whole genome shotgun sequence.
ACCESSION   DATABV010000089 DATABV010000000
VERSION     DATABV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348485"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1587
                     /gene="argH"
                     /locus_tag="VHF88_03700"
     CDS             217..1587
                     /gene="argH"
                     /locus_tag="VHF88_03700"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX2160905.1"
                     /translation="MSRFSEPQDPLFQALNSSIGFDFRLWPYDIAQSQAHARMLAASG
                     IIGAEDAAEIARGLDQVRDEIESDRFEIRPDDEDVHMAIERRLTELTGDAGERLHTAR
                     SRNDQVATDVAMLVRAHTLDAKSLLAELMGTLVELAERHLDWPLPGYTHLQRAQPVYL
                     SHHLLAYFWKFRRDLQRFNFCMNATDDLPLGAGALAGTNFATDRMLVAQELGFGGIAE
                     NSLDAVSNRDFVLDYLSAAATCATHLSQLGAEIVLWSSEEFGFCQVADRFASGSSLMP
                     QKKNPDAAELLRAKAPRVAGRLTTLHGVLHGLPLTYNKDLQEDKEQLFDAIDTVELCL
                     RVAREMLATISFDQQRMAAAATDELLAATDVADLLVKRGVPFRRAHGIVGGLVRTAVE
                     QGKALSQLSESELAELAPELDGEYYALLGDRAWLESKVSDGGTSLARVQDQLAQARHL
                     LAEAAA"
     gene            1596..2159
                     /locus_tag="VHF88_03705"
     CDS             1596..2159
                     /locus_tag="VHF88_03705"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2160906.1"
                     /translation="MPAAFYERPVVEVAGDLLGCVVRHGDTAGRIVETEAYHHSEPAC
                     HAYVGLTPRTRVLFGPPGVAYVYRSYGIHALLNAVCEPEGEGAAVLIRALEPVEGIDL
                     MRERRGLDGIRDLCSGPGKLTQALGIDLDLNDTPLDGPISIHARPPGEPPPSAACERI
                     GITRAVELPWRFCELGSRYLSRPVPER"
BASE COUNT          299 a          847 c          744 g          309 t
ORIGIN      
        1 caccgagccc gggtcggtca cgtcgccgcg caccgcctcg acgccttcgg gcagcacggc
       61 cgctgcccgc gccgggtcgc gcatgagcgc gacgacgtcg tcgccgcgct cggccagcgc
      121 ccgcgccacc gcgttgccga ccttgcccgc cgctcccgtc accagtgccc gcatcggggg
      181 cacgctatcc gaccgctgct cgtagactgg cgccccgtgt cgcgcttctc cgagccgcag
      241 gaccccctct tccaggcgct gaactcctcg atcgggttcg acttccggct ctggccgtac
      301 gacatcgccc agtcgcaggc ccacgcgcgg atgctcgcgg cgagcgggat catcggcgcg
      361 gaggacgcgg ccgagatcgc ccgcggcctc gaccaggtcc gcgacgagat cgagtccgac
      421 cgcttcgaga ttcgcccgga cgacgaggac gtccacatgg cgatcgagcg gcggctgacc
      481 gagctgaccg gcgacgccgg cgagcgcctg cacaccgcca ggtcgcgcaa cgaccaagtg
      541 gcgaccgacg tcgcgatgct cgtccgggcc cacacgctcg acgccaagtc gctgctcgcc
      601 gagctgatgg gaacgctggt cgagctcgcc gagcgccacc tcgactggcc gctgcccggc
      661 tacacgcacc tccagcgcgc ccagccggtc tacctctcgc accacctgct ggcctacttc
      721 tggaagttcc gccgcgacct gcagcggttc aacttctgca tgaacgcgac ggacgacctg
      781 ccgctcgggg cgggcgcgct ggcgggcacg aacttcgcga ccgaccggat gctggtcgcc
      841 caggagctgg gctttggcgg catcgccgag aactcgctcg acgcggtctc gaaccgcgac
      901 ttcgtgctcg actacctgtc cgccgccgcg acctgcgcca cgcacctgtc gcagctgggt
      961 gccgagatcg tgctctggtc gagcgaggag ttcggcttct gccaggtcgc cgaccgcttc
     1021 gcgtccggat cgagcctgat gccgcagaag aagaacccgg acgctgcgga gctgctgcgc
     1081 gccaaggccc cgcgcgtcgc cgggcggctg acgaccctcc acggcgtcct gcacgggctt
     1141 ccgctgacct acaacaagga cctccaggag gacaaggagc agctcttcga cgcgatcgac
     1201 acggtcgagc tgtgcctgcg cgtcgcgcgc gagatgctcg ccacgatctc cttcgaccag
     1261 cagcggatgg cggccgcggc gaccgacgag ctgctcgccg cgaccgacgt cgccgacctg
     1321 ctcgtcaagc gcggcgtccc gttccgccgt gcgcacggca tcgtcggggg cctcgtgcga
     1381 acggcggtcg agcagggcaa ggcgctctcg caactgagcg agtccgagct cgcagagctc
     1441 gcgcccgagc tcgacggcga gtactacgcg ctgctcggcg accgtgcctg gctcgagtcg
     1501 aaggtctccg acggcggcac ctccctcgcc cgcgtccagg atcagctggc gcaggcgcgc
     1561 cacctgctcg cggaggcggc ggcctgacgg aggttctgcc tgcggcgttc tatgagcgcc
     1621 ctgtcgtgga ggtggcgggc gacctgctcg gctgcgtggt gcgccacggc gacaccgccg
     1681 gccggatcgt cgagacggag gcctaccacc actccgagcc ggcctgccac gcctatgtcg
     1741 gcctgacccc gcgcacgcgg gtgctgttcg gtccgccggg cgtcgcctac gtctaccgct
     1801 cctacggcat ccacgccctg ctcaacgccg tctgcgagcc cgagggggag ggtgccgcgg
     1861 tgctgattcg cgcgctcgag ccggtcgagg gcatcgacct gatgcgcgag cgacgcggat
     1921 tggacggaat ccgcgacctt tgctccggcc ccggcaagct cacccaggcg ctcggcatcg
     1981 acctcgacct caacgacacg ccgctcgacg gcccgatctc gatccacgcg cgcccgcccg
     2041 gcgagccgcc accgagcgcg gcgtgcgagc ggatcggcat cacgcgcgcc gtcgagctgc
     2101 cctggcgctt ctgcgagctg ggcagccgct acctgtcgcg gccggtcccc gagcgctgat
     2161 ggtctattgg ggcgcggccg ctccgccgcc accgcccgg
//
LOCUS       DATABV010000090         4647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2036239, whole genome shotgun sequence.
ACCESSION   DATABV010000090 DATABV010000000
VERSION     DATABV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036239"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VHF88_03710"
     CDS             complement(<1..841)
                     /locus_tag="VHF88_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX2160907.1"
                     /translation="MLDLKAIRSEPDAARAALARRGDGSDERLTRVLALDERRRAILP
                     ELEGLRAEKNAASKQIGELQRGGGDASQAIAAVKDVGERQKALDAELKAVEGELEGAL
                     ASLPNLPDPDAADSDTVLREVGAADATGRDHLELLDGLVDLEAGARVAGSRFAYLKGE
                     LVFLELALVRWALGLLGERGFTPVVPPVLVREEALYGTGFLPDTEQQIYRVPDDDLYL
                     SGTSEVALAGLHMGEVLEDADVPARYAGFSTCFRREAGAAGRDTRGIFRVHQFDKVEM
                     FAFV"
     gene            complement(884..1318)
                     /locus_tag="VHF88_03715"
     CDS             complement(884..1318)
                     /locus_tag="VHF88_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160908.1"
                     /translation="MTRPATRIRARRGLYAVVAVALLGASGCAKGEIDVDPENRRAAN
                     LFYERCSGCHTFEPANSYGSKQAGELAPGERTNGPNFNVRKVSKEDALYAIRNGGFSG
                     AIMPAHIVMGEDAEQVADFLAKYSGRDRGEGTDVQGSGEAAE"
     gene            1345..1746
                     /locus_tag="VHF88_03720"
     CDS             1345..1746
                     /locus_tag="VHF88_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2160909.1"
                     /translation="MTMPLGRILCATGAVLALAGCGEAEEPPEVAAAVSAGKPLTVVA
                     REYLFSPNRVTIKGAPADGTRQRIELENRGELAHNIEILEGDRIVNRLRSFPAGQRRG
                     LTVDLPRGEYRFICTVADHDEKGMHGTLTVR"
     gene            complement(1748..2119)
                     /locus_tag="VHF88_03725"
     CDS             complement(1748..2119)
                     /locus_tag="VHF88_03725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2160910.1"
                     /translation="MTIDAKPERLGEAREWAQRTAAEAGLDEGDCYQVKLAISEAVAN
                     AIQHGSQNSGDCIRIAAFESDGSLVFEVQDSGTFVEPMSRATTEDESGRGLELMTLMM
                     DEVHMTSTGDGSVLRFAKRLN"
     gene            complement(2253..2666)
                     /locus_tag="VHF88_03730"
     CDS             complement(2253..2666)
                     /locus_tag="VHF88_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160911.1"
                     /translation="MAVGIAVLATNAVAGVWGAVAWLRKDPSVWFWYLLRGAQAAVVV
                     QAVLGFGLLAQGLRAPDGLHAAYGFAPLLITLISEGMRVGVAQRELEGVDIDSLQRGE
                     QVTIARRVALAEMGVMTIGVLMILTLALRAYQTGA"
     gene            2776..3132
                     /gene="ndhC"
                     /locus_tag="VHF88_03735"
     CDS             2776..3132
                     /gene="ndhC"
                     /locus_tag="VHF88_03735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX2160912.1"
                     /translation="MLSAYLPALVFLALGIAVGTVFALANGALGPRRPGKTKSEPYEC
                     GLPSDVRKSFRFGISFYLIAMLFILFDIEVVFLYPIAVQVQEAGVAALVKVAIFVSLL
                     LVAFLHQWRRGSLEWK"
     gene            3123..3758
                     /locus_tag="VHF88_03740"
     CDS             3123..3758
                     /locus_tag="VHF88_03740"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX2160913.1"
                     /translation="MEVTRTKVPGPGPGSESPEAFRAKQLSAQQMLRGDLDGRALEEY
                     VEERVMLTTLEKAQNWARRNACWPLGFGLACCAIEMITVIGSPRNDISRFGAEVVRAS
                     PRQADMIILSGRVSIKMAPVIRRIYDQMLEPKWVIAMGVCSSSAGMFNNYAVVQGADK
                     FMPVDVYIPGCPPRPEALMYGINKLQDQITNDPSLGWRERYQAEGTEERSA"
     gene            3751..4272
                     /locus_tag="VHF88_03745"
     CDS             3751..4272
                     /locus_tag="VHF88_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2160914.1"
                     /translation="MPDAAGLELVAQRVRAAVADDAVVGTSYAHEQALLEVAPARVRE
                     VLAYLRDGDEEPYEFLASLHAVDYLPDEPRLGIHYQLLSRPRVERLGVKTRVTVEDPV
                     VPSVVDLFPTANHQEREAFDMFGVRFDGHPDPRRILMPEDYEGHPQRRDFPVGGEPVL
                     FTHNEHELPRWYD"
     gene            4265..>4647
                     /locus_tag="VHF88_03750"
     CDS             4265..>4647
                     /locus_tag="VHF88_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160915.1"
                     /translation="MTDEPLAPGTAATPDPLATPYRERERSVTIERVAHGSVVPTEEL
                     EPETELFTINLGPHHPATHGVLRLLCTLEGETVREVKPIIGYVHTGIEKSMETMQYWK
                     AITFVERMDYLAYYFNAQAFCMTTER"
BASE COUNT          691 a         1700 c         1563 g          693 t
ORIGIN      
        1 gcacgaacgc gaacatctcg accttgtcga actggtggac gcggaagatc ccgcgcgtgt
       61 cgcggccggc ggcgccggcc tcgcggcgga agcaggtcga gaagccggcg tagcgggccg
      121 gcacgtcggc gtcctcgagg acctcgccca tgtgcaggcc ggctagcgcc acctccgagg
      181 taccgctgag gtagaggtcg tcgtcgggca cgcgatagat ctgctgctcg gtgtcgggca
      241 ggaagcctgt cccgtagagc gcctcctcgc gtacgagcac cggcggcacg accggcgtga
      301 agccgcgctc gccgagcagc ccgagcgccc agcgcacgag cgccagctcg aggaagacga
      361 gctcgccctt gaggtaggcg aagcgcgagc ccgccacccg ggcaccggcc tcgaggtcga
      421 cgagcccgtc gagcagctcc aggtggtcgc gccccgtcgc gtccgccgcg ccgacctcgc
      481 gcagcaccgt gtcggagtcc gcggcatcgg ggtcgggcag gttcggcagc gacgccagcg
      541 caccctccag ctcgccctcg accgccttca gctccgcgtc gagcgccttc tgccgctcgc
      601 cgacgtcctt gaccgccgcg atcgcctgcg acgcgtcgcc gccaccgcgc tgcagctcgc
      661 cgatctgctt gctcgccgcg ttcttctcgg cgcgcaatcc ctcgagctcc ggcaggatcg
      721 cccgccgtcg ctcgtcgagc gcgagcacgc gggtcagccg ctcgtcggac ccgtcgccgc
      781 ggcgcgcgag cgccgcccgc gccgcgtcgg gctcggaccg gatcgccttg aggtcgagca
      841 tgcgaccggc tcggttagac gcggtggtcg acgtgcgccg aggtcactcg gcagcctcgc
      901 cagagccctg gacgtcggtg ccctcgcccc ggtcccggcc cgagtacttc gccaggaagt
      961 cggcgacctg ctcggcgtcc tcgcccatca cgatgtgcgc cggcatgatc gcgcccgaga
     1021 agccgccgtt gcggatcgcg tagagggcgt cctccttgct gaccttgcgc acgttgaagt
     1081 tcgggccgtt ggtgcgctcg ccgggggcga gctcgcccgc ctgcttggag ccgtaggagt
     1141 tggccggctc gaaggtgtgg cagccagagc agcgctcgta gaagaggttc gccgcgcggc
     1201 ggttctcggg gtcgacgtcg atctcgccct tggcgcagcc ggaggcgccc aggagagcga
     1261 cggcgacgac tgcgtagagc cctctgcggg cacggatgcg agtggcgggg cgggtcacgg
     1321 cgggcgcata gtatccgccg tccgatgacg atgcccctcg gacggatcct ctgcgcgact
     1381 ggcgcggtcc tggcgctggc cggatgcggc gaggccgagg agccgccaga ggttgcggcg
     1441 gcggtcagcg ccggcaagcc gctgacggtc gtcgcccgtg agtacctctt ctcgcccaac
     1501 cgcgtgacga tcaagggcgc gccggctgac ggcacgcgcc agcggatcga gctcgagaac
     1561 cgcggcgagc tcgcccacaa catcgagatc ctcgagggcg accggatcgt caaccggctg
     1621 cgcagcttcc ccgccggcca gcgacgcggc ctgaccgtcg acctcccgcg cggcgagtac
     1681 cgcttcatct gcaccgtcgc cgaccacgac gagaagggca tgcacggcac gctcaccgtg
     1741 cgctgagtca gttcaggcgc ttggcgaatc gaaggacgct gccgtcgcca gtcgaggtca
     1801 tgtggacctc gtccatcatc agcgtcatca gctcgagtcc gcggccgctc tcgtcctcgg
     1861 tcgtggcgcg gctcatcggc tcgacgaagg tgccgctgtc ctgcacttcg aagaccagcg
     1921 atccgtcgga ctcgaaggcg gcgatacgga tgcagtcgcc gctgttctgc gagccgtgct
     1981 ggatcgcgtt ggcgacggcc tcgctgatcg caagcttcac ctggtagcag tcgccctcgt
     2041 ccaggcccgc ctccgccgcg gtccgctgag cccactcgcg cgcctccccg aggcgctctg
     2101 gcttggcgtc gatcgtcatc gattgctcct cgaacacggg ccgagcagcc tcatcccggc
     2161 cctgggtcga gctccgttgg ccacatcagg gacaccctgc ccaatgccgc gttctacgaa
     2221 tcacgcgatc tggcgcagaa atgcccgccg actcaggcgc cggtctgata ggcgcgcagg
     2281 gcgagcgtca ggatcatcag gacgccgatc gtcatcacgc ccatctccgc gagtgcgaca
     2341 cgccgcgcga tcgtgacctg ctcgccgcgc tgcaggctgt cgatgtcgac tccctccagc
     2401 tcccgctgcg cgacgccgac gcgcatcccc tcgctgatca gggtgatcag cagcggcgcg
     2461 aagccgtacg cggcatggag cccgtccggc gcgcgcaggc cctgagctag gagcccgaat
     2521 ccgagcaccg cctggacgac gactgcggcc tgcgccccgc gcagcaggta ccagaaccag
     2581 accgatgggt ccttgcgcag ccaggccacg gcgccccaga cgccggcgac cgcgttggtc
     2641 gccaagacgg cgatcccgac cgccatgtgg acctccgtca agaccccgat cccgctgagg
     2701 gtgacggctc gtcgctagcg ccctttcgca ctatgtagca actccgcaca aacctcgtta
     2761 tacatccgtt ccccagtgct cagcgcctac cttcccgccc tcgtcttcct cgccctcggg
     2821 atcgccgtcg gcacggtctt cgcgttggcc aacggcgcgc tcggcccgcg acgccccggc
     2881 aagacgaaga gcgagccata cgagtgcggc ctaccgtcgg acgtccggaa gagcttccgc
     2941 ttcgggatca gcttctacct gatcgcaatg ctgttcatcc tgttcgacat cgaggtcgtc
     3001 ttcctctacc cgatcgccgt ccaggttcag gaggccggcg tcgcggcgct cgtgaaggtc
     3061 gcgatcttcg tcagcctgct gctggtcgcg ttcctgcacc agtggcggcg ggggtcgctc
     3121 gagtggaagt gacgcgcacc aaggtgcccg ggcccggccc tggttcggag tccccggagg
     3181 cgttccgcgc caagcagctc agcgcccagc agatgctgcg cggcgacctc gacggccgcg
     3241 cgctcgagga gtacgtcgag gagcgcgtga tgctgacgac gctcgagaag gcgcagaact
     3301 gggcgcgccg caacgcctgc tggccgctcg gcttcggcct cgcctgctgc gcgatcgaga
     3361 tgatcacggt gatcggctcg ccgcgcaacg acatctcgcg cttcggtgcc gaggtcgtgc
     3421 gcgcctcgcc gcgccaggcc gacatgatca tcctgtcggg ccgggtctcg atcaagatgg
     3481 cgcctgtcat ccgccgcatc tacgaccaga tgctcgagcc gaagtgggtg atcgcgatgg
     3541 gcgtctgctc gtcgagcgcc ggcatgttca acaactacgc cgtcgtccag ggcgccgaca
     3601 agttcatgcc ggtcgacgtc tacatccccg gctgcccgcc gcggcccgag gccttgatgt
     3661 acgggatcaa caagctgcag gaccagatca ccaacgaccc ctcgctcggc tggcgcgagc
     3721 ggtaccaggc ggaggggacc gaggagcgca gtgcctgacg cggccgggct cgagctggtc
     3781 gcccagcggg tgcgcgccgc ggtggccgac gacgccgtgg tcggcacgag ctacgcccac
     3841 gagcaggcgc tgctcgaggt ggctcccgcg cgcgtgcgcg aggtgctcgc atacctgcgc
     3901 gacggcgacg aggagcccta cgagttcctc gccagcctgc acgccgtcga ctacctaccc
     3961 gacgagcccc ggctcgggat ccactaccag ctgctctcgc gcccgcgcgt cgagcggctc
     4021 ggcgtcaaga cacgcgtgac ggtcgaggac ccggtcgtgc cgagcgtcgt cgacctgttt
     4081 ccgaccgcga accaccagga gcgcgaggcc ttcgacatgt tcggggtgcg gttcgacggc
     4141 catcccgacc cgcgccggat cctgatgccc gaggactacg agggtcaccc gcagcggcgc
     4201 gacttcccgg tcggcggcga gccggtcctc ttcacccaca acgagcacga gctgccgcgc
     4261 tggtatgact gacgagccac tggcacccgg gaccgcggca acgcccgatc cgctggcgac
     4321 gccctaccgc gagcgcgagc gctcggtgac gatcgagcgc gtcgcccacg gctcggtcgt
     4381 cccgaccgag gagctcgagc ccgagaccga gctcttcacg atcaacctcg ggccgcacca
     4441 ccccgccacc cacggcgtgc tgcgcctgct ctgcacgctc gagggcgaga ccgtgcgcga
     4501 ggtcaagccg atcatcggct acgtccacac cggcatcgag aagtcgatgg agacgatgca
     4561 gtactggaag gcgatcacgt tcgtcgagcg gatggactac ctcgcctact acttcaacgc
     4621 gcaggcgttc tgcatgacga ccgagcg
//
LOCUS       DATABV010000091        12030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_750534, whole genome shotgun sequence.
ACCESSION   DATABV010000091 DATABV010000000
VERSION     DATABV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12030
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750534"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1408
                     /locus_tag="VHF88_03755"
     CDS             77..1408
                     /locus_tag="VHF88_03755"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX2160916.1"
                     /translation="MDRAPVPDATVLIRAAQLLDPRIGLDQPGDLLIRDGEIAEIGAP
                     SSLAAPDGAEVVEAERLHAFPAFVDPHVHLRTPGREDEEDIETGTRAAAAGGYCAILA
                     MPNTDPVVDSAPILRSLRERARAEARIPTGFTASITVGMQGERLTEMAELADAGAAAF
                     TDDGLPVRSAGIMRHALRYQQLSGCVLALHEEDPTLSAGGVMHEGEVSAMLGLAGIPS
                     VSESTMVARDCELAGYEDGRIHVQHVSARRTVEVLERAKAAGVEVTAEASPHHLLLTH
                     DAVRSLDTHYKMNPPLRSEDDRQALIDGLRTGVLDCIATDHAPHSREEKEEPFELAPM
                     GVTGLESAFAAIHSDLVLPGVLDLGLVVERMTAGCEPFGLVVPTLAKGAPANVCLVDL
                     DAVWEVGEAGYESRSANCAFGGRRLTGRVRMTVAAGAVAFRERSFAIEAVR"
     gene            1405..2532
                     /gene="carA"
                     /locus_tag="VHF88_03760"
     CDS             1405..2532
                     /gene="carA"
                     /locus_tag="VHF88_03760"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX2160917.1"
                     /translation="MSAYLLLEDGTRLDGVAAGAPIAATGEVVFNTAMSGYQESVTDP
                     SYRGQVIVFTYPLIGNYGVAAENMESDAIHARAVVMREGVDREDVPHAEGGWLTWLAD
                     CGVPAIAGVDTRALVRHIRDRGAMRGGVFPGDMPEAEAMERVRAEPAMAGADLAREVT
                     PNAPVLLDPLTDTAGPTIAAIDTGIKDSIVRHLRERGARIELHPCTTSADELLARDAD
                     AFFLANGPGDPAALGYVVDTVRELIGKRPVFGICLGHQLLCRAVGLETTKLPFGHRGA
                     NHPVKDLETGRIDITSQNHGFAVVGPDGANTIDTDEPVRWETDFGAAELSQINLYDRT
                     VEGLVLRDVPGATVQYHPEAGPGPHDSLHIFDRFLRMVELV"
     gene            2532..5759
                     /gene="carB"
                     /locus_tag="VHF88_03765"
     CDS             2532..5759
                     /gene="carB"
                     /locus_tag="VHF88_03765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2160918.1"
                     /translation="MPRRDDIRKILVIGSGPIVIGQAAEFDYSGAQACKVLLEEGYEV
                     VLVNSNPATIMTDPEFATATYIEPLLPDSVARVIERERPDALLPTLGGGTALNLARDL
                     TDDGTLERYGVELIGANYDAIQRAEDRDLFKETMAKAGLRTARSAAVTSVQEALDALP
                     DIGLPAIVRPGFTMGGEGGGIARTEAEYRQVVGEGLAASPIGQVLVEESVIGWGEFEL
                     EVMRDHNDNVVIVCSIENIDPMGVHTGDSVTVAPQQTLSDRQYQLLRDQAIAVIRAVG
                     VETGGSNIQFAVNRETDEIVVIEMNPRVSRSSALASKATGFPIAKIAARLAVGYALEE
                     IDNDITRVTPASFEPTIDYVVVKWPRFAFEKFPGANGSLSTYMQSVGEAMAIGRTFKQ
                     AFVKAMRSREIDVVPSPPEDTDVLLTALETPGHDRYELLFEAVRRGISDDELCARTNI
                     DPWYMAEMTAIARGEDPEADLVRTFKAVDTCAAEFEAATPYYYSGWERPGRDGPRHEV
                     RRGDNASVVILGSGPNRIGQGIEFDYCCVHAAMTVRQSGRDAVMVNCNPETVSTDYDT
                     SDRLYFEPLTLDDVLGVIDIEQPEGVIVQFGGQTPLKLAQGLTDAGVKLLGTPVESIH
                     RAEDRPSFGALLEELELKSPPYATAATPEEALTAADHVGYPLLVRPSYVLGGRAMEIC
                     YSREALADYLRRHAPARQAGAEGALNRPGAAEGGSGGAEPPRDIFLDRFLEDAIEVDV
                     DALCDGETAQIGAIMQHVEEAGVHSGDSACVIPAMSLGPDMLAQVEDATRRIALSLGV
                     VGLINIQFAVFGNDELYVIEANPRASRTVPFVSKAVGVPLAKIACRLMLGERLADMDL
                     PLPAHPGHVAVKEAVLPFGRFPLADARLGPEMKSTGEVMGIAADYPAAFGKAQAAAGA
                     ELPRGGSVFISVTDGDKSSATQLAASLHDLGFEVLATGGTAQAIRRMGVPVERIRKLS
                     EGSPNVVERIEAGEVDLVINTPTGSGARADGYEIRRAAIARGIPCITTMSGASAAQRA
                     IRSRLAGQDEPVSLQELHAATAPDDKSARRAGQVSLMGSGGGAAE"
     gene            5762..6577
                     /locus_tag="VHF88_03770"
     CDS             5762..6577
                     /locus_tag="VHF88_03770"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX2160919.1"
                     /translation="MAAEPRTLAPPERRIAEVKRVERIGAYDLIVARDPVGPADPQPG
                     QFYMLASGERWGGGDRERPYLPRAFSFARVRRLPGGQGVDLSFLLEDVGPGTQRLGEL
                     AAGESLMLLGPLGVGFARPSQDRRPLLVGGGIGVAPILCWDDELRADGVEAAVLLGFR
                     SAGHAAAAALFGGAPDVSTDDGSAGRRALVTEPLRAELERDPHATVYACGPEPMLDAV
                     RGLCANLGVPAQLALEAGMACGYGACFGCVVATVDGYARVCVDGPVFAGEALA"
     gene            6574..7455
                     /locus_tag="VHF88_03775"
     CDS             6574..7455
                     /locus_tag="VHF88_03775"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX2160920.1"
                     /translation="MSEIDLCGVGLRGPILNGSGTFDAIAARRAFGDLLLDRFPFDCF
                     VSKTITLQPREGNPPPRLWETASGLVNSIGLPNKGVRRFLELDLPLLAELPVPLCVSV
                     MGFSHEELARLVEEIEALDEISLIELNLSCPNVETGTLVGADPEETAAALTAARASTD
                     KPLIAKLSPSATDPAAVAAAAELAGADAISLINTLKGMPLHPTTREPWLGGRTGGLSG
                     PAVRVVALEQVASVAARVDVPVIGMGGISCGRDAADLLAAGASAVAVGTENFRDPAAG
                     RRIAAELAAFEQKVPAK"
     gene            7575..7955
                     /gene="mihF"
                     /locus_tag="VHF88_03780"
     CDS             7575..7955
                     /gene="mihF"
                     /locus_tag="VHF88_03780"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX2160921.1"
                     /translation="MPVAPESLAQTQAKGGSDSRNGQAGITPERSLTQRMDALKRANE
                     IRTERARLKRDLKAGRIQIHGLLLDPPDYLTTAKVFDLLLAVPKYGRVKVNRILTHCR
                     ISPSKTIGGLSERQRNELVSYLRR"
     gene            7969..8544
                     /gene="gmk"
                     /locus_tag="VHF88_03785"
     CDS             7969..8544
                     /gene="gmk"
                     /locus_tag="VHF88_03785"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX2160922.1"
                     /translation="MDTPVLVITGPSGVGKGTLIRRLLEDFPGAELSVSATTRDPRNG
                     EVKGRDYHFLAPEEFDDRVARGEFVEHAEYAGNRYGTLRSELQRPARLIVLEIDLQGA
                     RQVRHAMPEAEQVFIAPPRDDLGELERRLRARGAESPEQIERRLAVAREELAAQDEFH
                     HRIVNDDAEEATRELIGLAATICAPEVRNES"
     gene            8541..8771
                     /gene="rpoZ"
                     /locus_tag="VHF88_03790"
     CDS             8541..8771
                     /gene="rpoZ"
                     /locus_tag="VHF88_03790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX2160923.1"
                     /translation="MINPRLDTLLEHVDSHYACVLVAAKRARQINSYYHNLGEGTFDE
                     YPPPMVETGSKNYLGIALEEIAQGKIKYRYRD"
     gene            8799..10061
                     /gene="coaBC"
                     /locus_tag="VHF88_03795"
     CDS             8799..10061
                     /gene="coaBC"
                     /locus_tag="VHF88_03795"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX2160924.1"
                     /translation="MARLLLGVSGGIAAYKAVELVRLATKAGHAVRVVQTPTSLRFVG
                     RATFEAVTGAPVLVDEFEEDPARGAFPGDPAPDHEPISHLELVRNCDAYVVAPASANT
                     LAKLAAGQADNLLTSAALACTAPLVVAPAMNGAMWAHPATEANVDVLRTRGAAVVEPA
                     TGELASKGEWGTGRLAEPVDILAAVERTIAQPALDDGELRGLRVLITAGGTREPIDAV
                     RYVGNRSSGRMGVALAGEATRRGAHVTLIGANLAVEPPPGVHLVEVATAAELEQATRA
                     EFARADVLLMAAAVADFRPAVAADGKIKKDERDDLTIHLEPTVDVLAAVAQERRPGQT
                     LVGFAAEHGDGALDNAHAKLERKRLDAIVLNDVSLPGIGFDSPDNEVTVLTAGAERVI
                     PRASKLSVASAILDCVESLRKTQSEVSQ"
     gene            10058..10438
                     /locus_tag="VHF88_03800"
     CDS             10058..10438
                     /locus_tag="VHF88_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2160925.1"
                     /translation="MSETPEERGERVYDLYKRGSDLLERGDFAAAAIPLERARQLEPD
                     KTSIREALGRAYFRSGRFGPAAEEFEAVVERAPVNDFAHFCLGKALVKTGRLREARRH
                     ATLAAGMRPDRADYRNLRDRLSPG"
     gene            complement(10425..10850)
                     /locus_tag="VHF88_03805"
     CDS             complement(10425..10850)
                     /locus_tag="VHF88_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160926.1"
                     /translation="MPAAVEVIDAYIAALPGERRRIAHGEWGLTLAAEQAGGWPLDVG
                     LRIADGLLTVKAHALTDPQHVDPWMLLWWNRSTRMARFSANQAQEIWVHGDLPATAID
                     EVALDRLLGLVVEGAIAVRDFQRAKREPPEPQGGWLSPG"
     gene            11000..11482
                     /gene="rimP"
                     /locus_tag="VHF88_03810"
     CDS             11000..11482
                     /gene="rimP"
                     /locus_tag="VHF88_03810"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX2160927.1"
                     /translation="MTTNTDQLHSDIEARLAGPLPLVELLSCERIGADRLRLTIDHPD
                     GVDLGLCERVTHELAELLVDYGLEVSSPGPERPLVKPDHFRRFLGRRARVRTREPRDG
                     HRNFTGELVGASDAEVTVAAGTGVVTIPYAEIARSHLVEGAGAAGDRTDDDTGMEATG
                     "
     gene            11479..>12030
                     /locus_tag="VHF88_03815"
     CDS             11479..>12030
                     /locus_tag="VHF88_03815"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX2160928.1"
                     /translation="MSKEIVEAIGALGQEKGIATDTLMDALADALLSAYKKSPGSAKY
                     ARVDIDHDSGDFTVYELILPPELEERLLAEAEEAQEEPEIDPETGELREPEEPELDPD
                     VLAEYSDQIEERDVTPSDFGRIAAQTAKQVILQRVREAERDMMFDEYQDRVGELITGL
                     VQQSDSRYTLVQLRERVEALLPRS"
BASE COUNT         1792 a         4367 c         4227 g         1644 t
ORIGIN      
        1 cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc
       61 cacggagtcc ggaaggttgg atcgggcgcc ggttccggac gccaccgtcc tgatccgcgc
      121 cgcccagctg ctcgaccccc gcatcggcct cgaccagccc ggcgacctgc tgatccgcga
      181 cggcgagatc gccgagatcg gggctcccag ctcgctggcc gctcccgacg gcgccgaggt
      241 cgtcgaggcc gagcgactgc acgccttccc ggccttcgtc gacccacacg tccacctgcg
      301 cacgccgggc cgcgaggacg aggaggacat cgagaccggc acgcgcgcgg cggcggcggg
      361 cggctactgc gcgatcctcg cgatgccgaa caccgacccg gtggtcgact cggcgccgat
      421 cctgcgctcg ctgcgcgagc gcgctcgcgc ggaggcgcgc ataccgaccg gcttcacggc
      481 ctcgatcacc gtcggcatgc agggcgagcg gctgaccgag atggccgagc tcgccgacgc
      541 gggcgcggcg gcgttcaccg acgacggcct gcccgtccgc tcggccggga tcatgcgcca
      601 tgcgctgcgc taccagcagc tgagcggctg cgtgctggcg ctgcacgagg aggacccgac
      661 gctctcggcc ggcggcgtga tgcacgaggg cgaggtctcg gcgatgctcg gcctcgccgg
      721 gatcccgtcg gtgtcggagt cgacgatggt cgcccgcgac tgcgagcttg ccggctacga
      781 ggacgggcgg atccacgtcc agcacgtctc ggcgcggcgc accgtcgagg tgctcgagcg
      841 cgccaaggcc gccggcgtcg aggtgacggc cgaggcctcg ccgcaccatc tgctgctgac
      901 ccacgacgcg gtccgctcgc tcgacaccca ctacaagatg aaccccccgc tgcgcagcga
      961 ggacgaccgc caggcgctga tcgacggcct gcgcactggc gtgctcgact gcatcgcgac
     1021 cgaccacgcg ccccactcgc gtgaggagaa ggaggagccg ttcgagctgg cgccgatggg
     1081 cgtcaccggc ctcgagtcgg cgttcgcggc gatccacagt gacctcgtgc tgcctggagt
     1141 gctcgacctc ggtctcgtcg tcgagcggat gaccgccggc tgcgagccgt tcggcctggt
     1201 cgtgccgacg ctcgccaagg gcgctccggc gaacgtctgc ctggtcgacc tcgacgcggt
     1261 ctgggaggtt ggcgaggccg gctacgagag ccgctcggcg aactgcgcct tcggcggccg
     1321 gcggctgacc ggccgcgtac ggatgaccgt cgccgcgggc gcggtcgcgt tccgcgagcg
     1381 ctcgttcgcg atcgaggccg tccggtgagc gcgtacctgc tgctggagga cggcacgcgc
     1441 ctcgacggcg tcgccgccgg tgcgccgatc gctgctacgg gcgaggtcgt cttcaacacc
     1501 gcgatgtccg gctaccagga gtcggtcacc gacccgagct accgcggcca ggtcatcgtc
     1561 ttcacctatc cgctgatcgg caactacggc gtggcggccg agaacatgga gtccgacgcg
     1621 atccatgccc gcgcggtcgt gatgcgcgag ggggtcgatc gcgaggacgt cccgcacgcc
     1681 gagggcggct ggctgacgtg gctggcggac tgcggcgtgc cggcgatcgc cggagtcgac
     1741 acgcgcgcgc tggtgcgcca catccgcgac cgcggcgcga tgcgcggcgg cgtcttcccc
     1801 ggcgacatgc cggaggccga ggcgatggag cgcgtccgcg cggagcccgc gatggccggc
     1861 gccgacctcg cccgtgaggt gaccccgaac gccccggtgc tgctcgaccc gctgaccgac
     1921 acggccggcc cgacgatcgc cgcgatcgac acgggtatca aggactcgat cgtccgccac
     1981 ctgcgcgagc gcggagcgcg gatcgagctg cacccctgca cgacgagcgc cgacgagctg
     2041 ctggcgcgcg acgccgacgc cttcttcctc gccaacggcc ccggcgatcc ggcggcgctc
     2101 ggctatgtcg tcgacacggt gcgcgagctg atcggcaagc ggccggtgtt cgggatctgc
     2161 ctcggccacc agctgctctg ccgtgcggtc ggcctggaga ccaccaagct gccgtttggg
     2221 catcgcggcg ccaaccaccc ggtcaaggac ctcgagaccg ggcggatcga catcacgagc
     2281 cagaaccacg gcttcgccgt cgtcggaccc gacggcgcga acacgatcga caccgacgag
     2341 ccggtccgct gggagaccga cttcggcgcc gccgagctgt cgcagatcaa cctctacgac
     2401 cgcaccgtcg aggggctggt gctgcgcgac gtccccggcg cgacggtcca gtaccacccc
     2461 gaggcgggcc ccggcccaca cgactcgctg cacatcttcg accgcttcct gcggatggtc
     2521 gagctcgtct gatgccgcgc cgcgacgaca tccgcaagat cctcgtgatc ggcagcggtc
     2581 cgatcgtgat cggccaggcg gccgagttcg actactcggg cgcgcaggcc tgcaaggtgc
     2641 tgctcgagga gggctacgag gtagtcctgg tcaactcgaa cccggcgacg atcatgaccg
     2701 acccggagtt cgcgaccgcg acctacatcg agccgctgct gccggactcg gtggcgcgcg
     2761 tgatcgagcg cgagcgcccc gatgcgctgc tgccgacgct cggcggcggc accgcgctca
     2821 acctggcccg cgacctgacc gacgacggca cgctcgagcg ctacggggtc gagctgatcg
     2881 gcgcgaacta cgacgcgatt cagcgcgccg aggaccgcga cctgttcaag gagacgatgg
     2941 cgaaggccgg gctgcgcacc gctcgctcgg cggccgtcac gtcggtgcag gaggcgctcg
     3001 acgcgctgcc cgacatcggc ctgccggcga tcgtccggcc gggcttcacg atgggcggcg
     3061 agggcggcgg catcgcgcgc accgaggccg agtaccggca ggtcgtgggc gaggggctcg
     3121 cggcgagtcc gatcggccag gtgctcgtcg aggagtcggt gatcggctgg ggcgagttcg
     3181 agctcgaggt gatgcgcgac cacaacgaca acgtcgtgat cgtctgctcg atcgagaaca
     3241 tcgacccgat gggcgtccac actggggact cggtcacggt ggcgccgcag cagacgctgt
     3301 ccgaccgcca gtaccagctg ctgcgcgacc aggcgatcgc cgtcatccgg gcggtcggcg
     3361 tcgagaccgg cggctcgaac atccagttcg cggtcaaccg cgagaccgac gagatcgtcg
     3421 tgatcgagat gaacccgcgc gtctcgcgct cgtcggcgct cgcctcgaag gcgaccggct
     3481 tcccgatcgc caagatcgcg gcccggctcg cggtcggcta cgcactcgag gagatcgaca
     3541 acgacatcac gcgcgtcacg cccgcgagct ttgagccgac gatcgactac gtcgtggtca
     3601 agtggccgcg cttcgccttc gagaagttcc cgggcgccaa cggctcgctc tccacctaca
     3661 tgcagtctgt cggcgaggcg atggcgatcg gccggacctt caagcaggcc ttcgtcaagg
     3721 ccatgcgctc gcgcgagatc gacgtcgtgc cgtcgccgcc cgaggacacc gacgtgctgc
     3781 tgaccgcgct cgagacccct ggccacgacc gctacgagct gctgttcgag gccgtgcggc
     3841 gcgggatcag cgacgacgag ctctgcgcca ggaccaacat cgacccgtgg tacatggcgg
     3901 agatgacggc gatcgcgcgc ggcgaggacc ccgaggccga tctcgtccgc accttcaagg
     3961 ccgtcgacac ctgtgcggcc gagttcgagg cggcgacgcc ctactactac tcgggctggg
     4021 agcggcccgg ccgagacggc ccgcgccacg aggtgcgccg gggagacaac gcctcggtcg
     4081 tgatcctcgg ctctggcccg aaccggattg gccaggggat cgagttcgac tactgctgcg
     4141 tccacgccgc gatgaccgtg cgccagtccg gccgcgacgc ggtgatggtc aactgcaacc
     4201 ccgagaccgt ctcgaccgac tacgacacct ccgaccggct ctacttcgag ccgctgacgc
     4261 tcgacgacgt gctcggcgtg atcgacatcg agcagccaga gggggtgatc gtgcagttcg
     4321 gcggccagac gccgctgaag ctcgcacagg gcttgacgga cgccggcgtg aagctgctcg
     4381 gaaccccggt cgagtcgatc caccgcgccg aggaccggcc gagcttcggc gcgctgctcg
     4441 aggagctcga gctcaagagc ccgccctacg cgacggccgc cacgcccgag gaggcgctga
     4501 ccgcggccga ccacgtcggc tacccgctgc tcgtccgccc gagctacgtg ctcggcggcc
     4561 gcgcgatgga gatctgctac tcccgcgagg ccctggcgga ctacctgcga cgtcatgctc
     4621 cggcacggca agctggagcc gagggcgcct tgaaccggcc aggcgccgcg gaggggggct
     4681 ccgggggggc ggagcccccc agggacatct tcctggatcg ctttctggag gatgcgatcg
     4741 aggtagacgt cgacgcgctc tgcgacggcg agacggcgca gatcggcgcg atcatgcagc
     4801 acgttgagga ggccggcgtc cactcgggcg actccgcctg cgtgatcccg gcgatgtcgc
     4861 tcgggccgga catgctggcc caggtcgagg acgcgacgcg caggatcgcg ctcagcctcg
     4921 gcgtcgtcgg cctgatcaac atccagttcg cggtcttcgg caacgacgag ctctacgtga
     4981 tcgaggccaa cccgcgcgcg tcgcgcaccg tcccgttcgt ctccaaggcg gtcggggtcc
     5041 cgctggcgaa gatcgcctgt cggctgatgc tcggcgagcg gctggcggac atggatctgc
     5101 cgctgcccgc tcacccaggg cacgtcgcgg tcaaggaggc ggtcctgccg ttcggccgct
     5161 tcccgctcgc cgacgcccgc ctcggcccgg agatgaagtc gaccggcgag gtgatgggca
     5221 tcgccgccga ctatcccgcc gccttcggca aggcccaggc cgccgccggc gccgagctgc
     5281 cgaggggcgg gagcgtcttc atcagcgtca ccgacggtga caagtcgagc gccacacagc
     5341 tcgccgcgtc gctccacgac ctcggcttcg aggtgctggc gacggggggg accgcccagg
     5401 cgattcgccg catgggcgtc ccggtcgagc ggatccgcaa gctcagcgaa ggctcgccga
     5461 acgtcgtcga gcggatcgag gcgggcgagg tcgacctcgt gatcaacacg ccgacgggct
     5521 ccggcgctcg cgccgacggc tacgagatcc gtcgcgccgc gatcgctcgc ggcatcccct
     5581 gcatcacgac gatgagcggc gcatcggcgg cccagcgagc gatccgctcc cggctcgccg
     5641 gtcaggacga gccggtctcg ctccaggagc tccacgccgc cacagcaccg gacgacaagt
     5701 ccgcacggcg ggccgggcag gtcagcctga tgggcagcgg gggcggagcc gcggagtgag
     5761 cgtggccgcc gagccccgga cgctcgcgcc ccccgagcga cggatcgccg aggtgaagcg
     5821 cgtcgagcgc atcggcgcct acgacctgat cgtcgcgcgc gatccggttg ggcctgccga
     5881 cccgcagccc ggccagttct acatgctcgc cagcggcgag cgctggggcg gcggcgaccg
     5941 cgagcgcccc taccttccac gcgccttttc cttcgcccgc gtgcgccgtc tgcccggtgg
     6001 gcagggggtc gacctgtcgt tcctgctcga ggacgtcggc ccaggcacgc agcggctcgg
     6061 cgagctcgcg gcgggcgagt cgctgatgct gctcggcccg ctcggcgtcg gcttcgcgcg
     6121 accgtcgcag gaccggcgac cgctgctggt cggcggcggc atcggggtcg cgccgatcct
     6181 ctgctgggac gacgagctgc gcgccgacgg cgtggaggct gccgtgctgc tggggttccg
     6241 ctcagccgga cacgccgcgg ccgcggcgct cttcggcggc gcccccgacg tctcgaccga
     6301 cgacgggtcg gcgggcaggc gcgcgctcgt caccgagccg ctgcgcgccg agctcgagcg
     6361 cgatccgcac gcgaccgtct acgcctgcgg gcccgagccg atgctcgatg cggtgcgcgg
     6421 gctctgcgcg aacctcggcg tcccggcgca gcttgcgctc gaggccggca tggcctgcgg
     6481 ctacggcgcc tgcttcggct gcgtggtggc gaccgtcgac ggctacgcgc gcgtctgcgt
     6541 cgacgggcct gtgttcgccg gggaggcgtt ggcgtgagcg agatcgacct ctgcggcgtc
     6601 gggctgcgag gaccgatcct caacggctcc ggcaccttcg acgcgatcgc cgcgcggcgc
     6661 gcattcggcg acctgctgct ggaccgcttc cccttcgact gcttcgtctc aaagacgatc
     6721 acgctccagc cgcgcgaggg caacccgccg cctcgattgt gggagaccgc gagtggcctg
     6781 gtcaactcga tcggcctgcc gaacaagggc gtgaggcgct ttctcgagct cgacctgccg
     6841 ctgctcgcgg agcttcccgt gccgctgtgc gtctcggtca tgggcttcag ccacgaggag
     6901 ctcgcccggc tcgtcgagga gatcgaggcg ctcgacgaga tctcgctgat cgagctcaac
     6961 ctctcctgtc cgaacgtcga gaccggcacg ctggtcggcg ccgaccccga ggagaccgca
     7021 gccgctctga ccgcggctag ggcctcgacc gacaagccgt tgatcgcgaa gctctcgccg
     7081 agcgccaccg accccgccgc cgtcgccgcg gctgccgagc ttgcgggcgc ggacgcaatc
     7141 tcgttgatca acacgctcaa gggcatgccg ttgcacccca cgacgcgtga gccgtggctc
     7201 ggcggccgca ccgggggcct gtcgggtccc gcggtgaggg tcgtggcgct ggagcaggtg
     7261 gcgtcggtag cggcgcgggt cgacgtgccg gtgatcggga tgggcggcat ctcctgcggt
     7321 cgcgatgctg ccgacctgct cgcggccggc gcctcggctg tagcagttgg cacagagaac
     7381 ttccgtgatc ccgccgcggg ccggcggatc gccgccgagc tcgccgcgtt cgagcagaaa
     7441 gtccctgcaa agtaactcca aagggtcaag gaagcccaag cttcctgaag ctcaagtcga
     7501 gatagaggtg aattcacccc aaaaaggcac tctcgggccg ccgtggccac gatccgtctg
     7561 tatagtcgcc gcccatgccg gttgcgcccg aatcgctcgc ccagacccag gccaagggcg
     7621 gcagcgactc caggaacggg caggccggca tcaccccgga gcgctccctg acccagcgca
     7681 tggatgcgct caagcgcgcc aacgagatcc gcaccgagcg ggcccgcctg aagcgcgatc
     7741 tgaaggccgg acggatccag atccacggcc tgctgctcga cccgcccgac tacctcacga
     7801 cggcgaaagt cttcgatctc ctgctcgccg tcccgaagta cggccgcgtc aaggtgaacc
     7861 ggattctcac ccactgccgc atctcgccga gcaagacgat cggcggcctc tcggagcgcc
     7921 agcgcaacga gctggtctcc tacctcaggc gctagccgaa agccagtcgt ggacacaccg
     7981 gtcctcgtga tcaccggccc atcgggcgtc ggcaagggga cgttgatccg ccgcctgctc
     8041 gaggactttc caggcgccga gctgtcggtc tcggcgacca cgcgcgaccc ccgcaacggc
     8101 gaggtgaaag ggcgcgacta ccacttcctg gcacccgagg agttcgacga ccgggtcgca
     8161 cgcggcgagt tcgtggagca tgccgagtac gccggcaacc gctacgggac cttgcgcagc
     8221 gagctccagc ggccggcacg gttgatcgtg ctcgagatcg atctccaagg cgcacggcag
     8281 gtccgtcatg cgatgccgga ggccgagcag gtcttcatcg ctccgccgcg cgacgacctc
     8341 ggcgagctcg agcgacgact gcgcgcgcga ggggcggaga gccccgagca gatcgagcgc
     8401 cggttggccg tcgcacgcga ggagctcgcg gcccaggacg agttccatca caggatcgtc
     8461 aacgacgacg ccgaagaggc cacgcgggag ttgatcggcc tcgccgctac catctgtgcc
     8521 cccgaagtca ggaacgaatc ttgatcaatc cccgtctgga cacgctgctg gagcacgtcg
     8581 actcgcacta cgcgtgcgtt ctcgtcgcgg cgaagcgtgc ccgtcagatc aacagctact
     8641 accacaacct cggtgaggga accttcgacg agtacccgcc gccgatggtc gagacggggt
     8701 ccaagaacta tctcgggatc gcgctcgagg agatcgcgca gggcaagatc aagtaccgct
     8761 accgggacta gcgtcccacc ggcgggaagg ccgaccagat ggcccgccta ctgctcggag
     8821 tgtctggagg gatcgcggcc tacaaggccg tcgagctcgt gcggctggcg acgaaggccg
     8881 gccatgcggt gcgcgtcgta cagacgccga cgagcctgcg gttcgtcggc agggcgacct
     8941 tcgaggcggt gaccggcgct ccggtgctgg tcgacgagtt cgaggaggat ccggcacgcg
     9001 gcgcatttcc cggcgacccg gcgccagacc acgagccgat ctcccacctc gagctggtgc
     9061 gcaactgcga cgcctacgtc gtcgcgccgg cctcggccaa cacgctcgcg aagctggcgg
     9121 cgggccaggc cgacaacctg ctgaccagtg cagcgctggc gtgtaccgcc ccgctcgtcg
     9181 tcgcgccggc gatgaacggc gcgatgtggg cccatcccgc gaccgaggcc aacgtagacg
     9241 tgctgcgcac gcgcggcgcc gccgtcgtcg agccggcgac cggagagctc gcgtcgaagg
     9301 gggagtgggg gaccggccgg ctcgccgagc cggtcgacat cctggccgcg gtcgagcgca
     9361 cgatcgccca gccggcgctg gacgacggcg agctgcgcgg gctgcgcgtg ctgatcaccg
     9421 ccggcggcac gcgcgagccg atcgacgcgg tgcgttacgt cggcaaccgg tcgtcgggac
     9481 ggatgggcgt ggcgctcgcc ggcgaggcga cgcggcgtgg cgcgcacgtg acgctgatcg
     9541 gcgcgaacct cgcggtcgag ccgccgcccg gcgtgcacct tgtggaggtc gccacggcgg
     9601 ccgagctgga gcaggctacg cgcgccgagt tcgctcgcgc cgacgtgttg ctgatggccg
     9661 ccgcggtcgc cgacttccgg cccgccgtag cggccgacgg caagatcaag aaggacgaac
     9721 gtgacgacct gacgattcat ctcgagccca ccgtcgacgt cctcgcggcg gtggcgcagg
     9781 agcgacggcc gggacagacg ctcgtcggct tcgccgccga gcatggggac ggcgcgctcg
     9841 acaacgcaca cgccaagctg gagcgcaagc ggctcgacgc gatcgtcctc aacgacgtct
     9901 cgctgccggg catcggcttc gatagccccg acaacgaggt caccgtgctc actgcgggcg
     9961 ccgagcgggt gatcccacgc gcctcgaagc tctccgtcgc gtccgccata ctcgattgcg
    10021 tcgagtcgct caggaagacg caaagcgagg tgagccagtg agcgagacgc cagaggagcg
    10081 cggcgagcgc gtctacgacc tgtacaagcg cggcagcgac ctgctcgaac gcggcgactt
    10141 cgcggcggcc gccatcccgc tcgagcgtgc acgccagctc gagccggaca agacctcgat
    10201 ccgcgaggcg ctgggtcgtg cctacttccg ctccggccgc ttcggtcccg cggccgagga
    10261 gttcgaggcg gtcgtcgagc gcgcccccgt gaacgacttc gcgcacttct gcctcggcaa
    10321 ggcgctcgtg aagaccggtc gcctgcgcga ggcccggcgc cacgcgacgc tggccgccgg
    10381 gatgcgcccg gaccgcgccg actaccgcaa cctgcgcgac cggctcagcc cgggctgagc
    10441 cacccgcctt gcggctcggg cggctcgcgc ttggcgcgct ggaagtcgcg cacggcgatc
    10501 gctccctcga cgacgaggcc gagcaggcgg tcgagcgcga cctcgtcgat ggccgtcgcc
    10561 ggcaggtcgc cgtggaccca gatctcctgc gcctggttcg cactgaagcg cgccatccgc
    10621 gtcgagcggt tccaccagag cagcatccac gggtcgacgt gctgtgggtc ggtcagagca
    10681 tgcgccttga cagtcaatag gccgtcggcg atgcgcagcc cgacgtccaa gggccagccg
    10741 cctgcctgct cggcggcgag cgtgaggccc cactcgccgt gggcgatccg cctgcgctcg
    10801 ccgggaagcg ctgcgatgta ggcgtctatg acctcgactg ccgcgggcac ctgcgagaca
    10861 ctaggggccg gcgctcgccg gcgtccacaa cggcgcgctg agcccgtcta tactggtccg
    10921 ctgctaggac tttcagacgt actgcgacct ccggtcgcgc ctggaggtgg gcttcgcagc
    10981 ccaccttttt ttcgacctga tgacgaccaa cacagaccag ctccacagcg acatcgaggc
    11041 ccgcctggcg ggtccgctgc cgctcgtcga gctgttgtcg tgcgagcgta tcggcgccga
    11101 ccgcctgcgc ctgacgatcg accatccgga tggcgtcgac ctcgggctct gcgagcgcgt
    11161 cacccacgag ctggcggagc tgctcgtcga ctacgggctc gaggtctcct cgcccggccc
    11221 cgagcgcccg ctggtcaagc ctgaccactt ccgtcgcttc ctcggccgcc gggcgcgcgt
    11281 gcggacccgc gagccgcgcg acggccaccg caacttcacc ggcgagcttg tcggcgcctc
    11341 ggacgccgag gtcaccgtcg ccgccgggac gggagtggtc acgatcccgt acgccgagat
    11401 cgcgcgcagc catctggtcg agggcgccgg cgcggctggc gatcgaaccg acgacgacac
    11461 gggaatggag gcaacgggat gagcaaggag atcgtcgagg cgatcggggc gcttggccag
    11521 gagaagggca tcgccactga cacgctgatg gacgcgctcg ccgatgcgct gctctcggcc
    11581 tacaagaaga gccccggttc ggccaagtac gcccgcgtcg acatcgacca cgacagcggc
    11641 gacttcacgg tctatgagct gatcctgccg cccgagctcg aggagcggct gctcgccgag
    11701 gccgaggagg cccaggagga gcccgagatc gatcccgaga ccggcgagct gcgcgagccc
    11761 gaggagccgg agctcgaccc cgacgtcctt gccgagtaca gcgatcagat cgaggagcgt
    11821 gacgtcacgc cgagcgactt cggccggatc gccgcccaga ccgccaagca ggtgatcctc
    11881 cagcgcgtgc gcgaggccga gcgcgacatg atgttcgacg agtaccagga ccgcgtcggc
    11941 gagctgatca ccggcctcgt gcagcagtcc gactcgcgct acacgctcgt ccagctgcgc
    12001 gagcgcgtcg aggcgctgct gccgcgctcc
//
LOCUS       DATABV010000092         6375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_268235, whole genome shotgun sequence.
ACCESSION   DATABV010000092 DATABV010000000
VERSION     DATABV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268235"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VHF88_03820"
     CDS             complement(<1..1086)
                     /locus_tag="VHF88_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160929.1"
                     /translation="MTTDDDSWLYTALAPARRLEPTDAELAPVTERVARSRRRRRRGR
                     RTVALAVPALLVGATAAAAATGLVSVGSVIPGDGFSGDERPVVKETVLVSGVDEEVGR
                     WRMTGFSTAQKADCLKLTLLERAARRAPGPAVSGYCGGLMPFSEFGHGRRAAAMARGE
                     VILFGTAPAAARTVELSGDGGVRVSVQTHSGVDASRRYWLLTAPTRLRDAELHWRDRR
                     DRAGGTLDVSHRFAGPSARTVVATGSTPAAGRWQLLAYESRRLSADGDVYSPEGLPCI
                     ELRLLDRAQVNARSGYCGIQRRTPGFTRGQSRLYRATGKVSGLIMFGRAPARADAVEV
                     SSGGRTISAPARPAPAGIPGRFWVLARP"
     gene            complement(1083..1673)
                     /locus_tag="VHF88_03825"
     CDS             complement(1083..1673)
                     /locus_tag="VHF88_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2160930.1"
                     /translation="MSTPPDLSDELRRFAAGDSAAAEALARHASRLALRTAAVIVGSR
                     DEAADVAQEVAIDVLGSVAKLREPAALDAWVHRITVRHAMRLLRRRGRARAVELPLAL
                     IGQADEPTAPEGPDRAALLTSRGALAAGLARLPARQRLALALRYVHDLSDEQIAQALG
                     CRVGTAHALLSRGRAALRSDPQLAELAPQTAIGDAR"
     gene            complement(1854..3296)
                     /locus_tag="VHF88_03830"
     CDS             complement(1854..3296)
                     /locus_tag="VHF88_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2160931.1"
                     /translation="MNFVQGGTMNVKRWTLAVVSAATAMLMLDIAVVNTALPRIAEDL
                     DTGLGGLQWVVDSYTLALASTVLTAGALADRLGRRRVFAVGLVVFTVASLACALAGSI
                     EVLNSARAVQGLGGAILFAVSLSLLANAYPDQRERAGALAVYGATIAGSFAVGPLVGG
                     ALTSAFDWQWVFLINIPIGLVCLWITLRCVEESRDPAARRIDWGGLVTLTGGLSLLVL
                     ALLRGNEDGWTSLPIVAELSGAALLLGAFLTIESRVREPMLPLGLFRDRAFSGAQIGA
                     FAISGSFFAVFIYATLYLQQILGLSPVEAGLVYVPSTIVMLVVSGATADLSEKVGPGV
                     LVSAGLALVAVGMAMITVADVDSSWAVMLPGLVVGMIGTGLFNPAVIAVALAGVPDEQ
                     SGLAAGVNDTFRQAGIAVGVAGLGALVPAGAAFGDGSPADYVAGLHDALWVGAGLAAA
                     GAVAAALLIARRGPAAAVEAPAQVEPVRAG"
     gene            complement(3394..4581)
                     /locus_tag="VHF88_03835"
     CDS             complement(3394..4581)
                     /locus_tag="VHF88_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2160932.1"
                     /translation="MNSRGPEGEVAVPSLGVGMLGHGFVGKAHAHALRTVAYMGWPPP
                     LAPRLVEVVGWRPEAVAEAARRYRFERATTDWRVLMEDPAIELFLNAGPNDLHAEPTL
                     AALRAGKHVLCEKPLGRTAPESHELWRAAAAAGVVHMCGFNYRFVPAVRLARELIEGG
                     ELGEIHSFRGTYFQDWLANPDSPYVWRLDRQRAGSGALGDIGAHLIDLARFLVGEIAA
                     VEGHVRTVVPERPGGLVDVDDTVDAMLEFEGGAGGSISASRLCHGRRNALGFEVSGTR
                     GTLAFELERMNELWVHLDDSRPGERAQGFRRVLVTDPGHPYLEHWWPPGHVIGWGDTF
                     VHEIHHLLRAIAGEDEVAPYGATFEDGYRAAEVCDAILRSSEQRACQRVAYTAAVDDV
                     GVR"
     gene            4788..5015
                     /locus_tag="VHF88_03840"
     CDS             4788..5015
                     /locus_tag="VHF88_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2160933.1"
                     /translation="MAEQSPLLLGLGNALREARAETRISQEELGLRTGVHRNYIGGIE
                     RGERNPSVTTIATLAEALGMKLSELFERAGS"
     gene            complement(5070..6290)
                     /locus_tag="VHF88_03845"
     CDS             complement(5070..6290)
                     /locus_tag="VHF88_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160934.1"
                     /translation="MVEASRARGKRALFGFAVAALAAALPAAATADGPGVGTPTVVTL
                     GDSAISGEAGRWAGNTNGSSSNVDALGPAAYHDVPGAESIPGCHRSQSAQAHIGGGVA
                     SANLACSGARTYTSGTASGEDFKPGIDFYSDASGRKGQALRLQEYAATHNVKAVVVMI
                     GANNYGFADIVQRCITNWLTSPSWWKNYCSDDSDMVSKFTPSAQAARTTEVRDALLRV
                     AQAMSNAGYSPSQYKILGQTYWSPLPRGAQIRYPESGWTRQSIGGCGTWNRDADWAND
                     TVVNALNNTMRNALATSGLTNTAVIDMQTALNGRRLCENTVGLLEEEGIANWTSPGAV
                     DNTEWVAQVRTVTTVFGPYQIQESMHASYWGQKAMRNCLRLAYNGGSPVGGRCVRASN
                     GLNVQGEPNMALVP"
BASE COUNT          955 a         2364 c         2227 g          829 t
ORIGIN      
        1 cggccgcgcg aggacccaga acctgcctgg gatgccggcc ggtgcgggcc gggccggcgc
       61 cgagatcgtc ctgccgccgg agctcacctc gacggcgtcg gcgcgcgcgg gcgcgcgtcc
      121 gaacatgatc agcccgctca ccttgccggt ggcgcgatag aggcgggact ggccgcgcgt
      181 gaagcccggg gtccgccgtt gaatgccgca gtagccgctt cgcgcattga cctgcgcacg
      241 atcgagcagc cgcagctcga tgcagggcag tccctccggc gagtacacgt cgccgtcggc
      301 ggacagacgc ctgctctcgt aggccagcag ctgccagcgc ccggcagcag gggtcgatcc
      361 ggtggcgacg actgtgcgcg ccgacggccc ggcgaagcgg tgcgagacgt cgagcgtgcc
      421 accggcccgg tcgcgccggt cgcgccagtg cagctcggcg tcgcgaaggc gagtcggcgc
      481 ggtcaagagc cagtagcgcc gcgaagcgtc cacgccggag tgcgtttgca cggagacccg
      541 cacgccgcca tcgccgctca gctcgacggt gcgggcggca gccggcgcgg tgccgaacag
      601 gatcacctcg ccacgcgcca tcgccgccgc acggcggccg tggccgaact cggagaacgg
      661 catcaggccc ccgcagtagc cgctgaccgc cggcccgggc gcccgccgcg cagctcgctc
      721 gagcagcgtc agcttcaaac agtccgcctt ctgcgccgtg ctgaagcccg tcatccgcca
      781 gcggccgacc tcctcgtcga cgccgctgac caggacggtc tccttgacga caggccgctc
      841 gtcgccgctg aagccatcgc ccgggatcac cgagccgacc gacaccagcc cggtggccgc
      901 cgcggccgcc gtcgcgccga ccagcagtgc cggcactgcg agcgcgacgg tccggcggcc
      961 gcgccgccgg cgacgtcgag atcgcgccac ccgctcggtc accggcgcga gctccgcgtc
     1021 ggtcggctcg agccggcgtg ctggcgccag agcggtgtac agccagctgt cgtcgtctgt
     1081 ggtcatcggg cgtctccgat cgcggtctgg ggtgccagct cggcgagctg cgggtcgctg
     1141 cgcagcgcgg cccggccgcg tgacagcagg gcgtgggcgg tgcccaccct gcagccgagc
     1201 gcctgggcga tctgctcgtc cgagaggtcg tggacatagc ggagcgcgag ggcgagccgc
     1261 tggcgcgccg gcagcctggc caggccagcg gccagggcac cgcgcgacgt gagcagcgcc
     1321 gcccggtccg gcccctccgg ggccgtcggc tcgtcagcct ggccgatcag cgccaacggc
     1381 agctcgaccg cgcgcgcccg cccccggcgg cgcagcaggc gcatcgcgtg gcgcaccgtt
     1441 atccggtgga cccaggcgtc gagcgccgcc ggttcgcgca gcttcgcgac cgacccgagc
     1501 acgtcgatcg cgacctcctg agcgacgtct gccgcctcgt cacggcttcc gacgatcacg
     1561 gcggcggtgc gcagcgccag tcgcgatgcg tgacgggcca gcgcctctgc cgcagcggaa
     1621 tcccccgcag cgaagcggcg cagctcgtcg gagaggtcgg gcggcgtgga catcgtggcg
     1681 actcgaatcc ctagtgcgcc gagccaccgg gaaaccttgc acgcgcgtgc ccacggttcg
     1741 gagtgtctcc ggccgggtac ctcgctgagg accgcctcac cgaggccccc gaccggagga
     1801 gactcacatg tacatcggaa tcggcaccgc gatcatcatc gtcatcctgc tgatcatcct
     1861 gctctgacgg gctccacctg ggctggcgcc tcgaccgcgg ccgcgggacc gcggcgggcg
     1921 atcagcaggg cggcggcgac cgctccggcc gccgccaggc cggcgccgac ccacagcgcg
     1981 tcgtgcaggc cggcgacata gtcggccggc gagccgtcgc cgaaggccgc gcccgcgggg
     2041 acgagcgctc cgagcccggc cacgccgacg gcgatccccg cctggcggaa cgtgtcgttg
     2101 acgccggcgg ccaggccgct ctgctcgtcc ggcacgccgg ccagggcgac cgcgatcacg
     2161 gcggggttga acagcccggt tccgatcatg ccgacgacca ggccgggcag catcaccgcc
     2221 catgacgagt cgacgtcggc gaccgtgatc atcgccatgc cgacggcgac cagcgcgagc
     2281 ccggccgaga cgagcacacc cggaccgacc ttctcggaga ggtccgccgt cgcccccgac
     2341 accacgagca tcacgatcgt gctcggaacg tagacgagtc cggcctcgac cggcgatagg
     2401 ccgaggatct gctgcaggta gagcgtcgcg tagatgaaga ccgcgaagaa cgaccccgag
     2461 atcgcgaacg cgccgatctg cgcgccgctg aaggcacggt cacggaacag gccgagcggg
     2521 agcatcggct cgcggacgcg gctctcgatc gtcaggaacg ctccgagcag cagcgccgcc
     2581 ccgctcagct cggcgacgat cggcaggctc gtccagccgt cctcgttgcc gcgcagcagc
     2641 gcgagcacca gcagcgacag cccgccggtc agcgtcacca ggccgcccca gtcgatccgt
     2701 cgcgcggccg ggtcgcgtga ctcctcgacg caacgcagcg tgatccagag gcagacgagc
     2761 ccgatcggga tgttgatcag gaagacccac tgccagtcga aggcgctggt caaggcgccg
     2821 ccgacgagcg ggccgaccgc aaacgagccg gcgatcgtcg ccccgtagac ggcgagtgcg
     2881 cccgcgcgct cgcgctggtc gggataggcg ttggcgagca gcgacagcga gaccgcgaac
     2941 aggatcgccc cgccgaggcc ctgcacggcg cgggcgctgt tgagcacctc gatgctgccg
     3001 gccagcgcgc aggccagcga cgcgaccgtg aagacgacca gcccgacggc gaagacgcgc
     3061 cggcggccga gccggtcggc gagcgcgccg gccgtcagga cggtgctggc cagcgccagc
     3121 gtgtacgagt cgacgaccca ctgcagcccg ccgaggcccg tgtcgaggtc ctccgcgatc
     3181 cgcggtagcg cggtgttgac cacggcgatg tcgagcatca gcatcgccgt cgcggccgag
     3241 accaccgcca gggtccagcg cttgacgttc atggttcctc cttggacgaa gttcatgtcc
     3301 aggcgaggat cggtccgcga gcggtcccgc acatcgaggg tgaccccaat cccgcacccc
     3361 agacttcgcc ctcccgaccc ctaggagctg gctctagcga accccgacgt cgtcgacggc
     3421 ggccgtgtag gcgactcgct ggcaggcgcg ctgctcgctc gagcgcagga tcgcgtcgca
     3481 gacctcggct gcgcggtagc cgtcctcgaa cgtcgccccg tacggtgcca cctcgtcctc
     3541 gccggcgatc gctcgcagga ggtggtggat ctcatggacg aacgtgtcgc cccagccgat
     3601 cacgtgccca ggcggccacc agtgctcgag gtaggggtgg cccggatcgg tgaccagcac
     3661 gcggcggaag ccctgcgcgc gctcgcccgg gcgcgagtcg tcgaggtgga cccacagctc
     3721 gttcatccgc tcgagctcga aggccagcgt gccgcgcgtg ccgctgacct cgaagccgag
     3781 cgcgttgcgg cggccgtggc agagacgcga ggccgagatc gatccgcccg cgccaccctc
     3841 gaactcgagc atcgcgtcga cggtgtcgtc gacgtcgacc agcccaccgg gacgctcggg
     3901 aacgaccgtc cgaacgtgcc cctcgacggc ggcgatctcg ccgaccagga agcgcgcgag
     3961 gtcgatcagg tgggcgccga tgtcaccgag cgcgcccgag ccggcgcgct ggcggtcgag
     4021 ccgccagacg tagggcgaat ccgggttggc gagccagtcc tggaagtagg tgccgcggaa
     4081 gctgtggatc tcgccgagct cgccgccctc gatcagctcg cgcgcgagcc gcaccgccgg
     4141 gacgaagcgg tagttgaagc cgcacatgtg cacaacgccg gcagcggccg ccgcccgcca
     4201 gagctcatgg ctctcgggcg ctgtgcggcc gagcggcttc tcgcagagca cgtgcttgcc
     4261 ggcgcgcagc gcggcgagcg tcggctcggc gtgcaggtcg ttcgggccgg cgttgaggaa
     4321 cagctcgatc gccgggtcct ccatcagcac gcgccagtcg gtcgtcgcgc gctcgaagcg
     4381 gtagcgccgc gcggcctcgg ccaccgcctc ggggcgccag ccgacgacct cgacgagccg
     4441 cggcgcgagc ggcggcggcc accccatgta tgccaccgtc cgcagggcgt gagcgtgcgc
     4501 cttgccaacg aagccgtgcc cgagcatccc gacgccgagg ctcggaaccg cgacttcgcc
     4561 ctcgggaccg cgtgagttca cccacggcca atgtgcccgc cgagcggata ggtaaccctc
     4621 gaccgcgaga acgaaaaagc cccccggcca aattggaggg agggccgggg ggcggctcac
     4681 ctaggtgagc acctacatgg tacaccaaat tacagaaaac caaaccatcg tgagaacacg
     4741 ctcaggctgt agatcccaca cggctgggct ggaaccgtct caggccgatg gccgagcagt
     4801 cgcccctact cctcggcctc ggcaacgccc tgcgcgaggc gcgcgccgag acccgcatat
     4861 cccaggagga gctgggcctg cgtaccggcg tgcaccgcaa ctacatcggc ggcattgagc
     4921 ggggcgagcg aaacccctcg gtgacgacga tcgccacgct cgccgaggcg ctcggcatga
     4981 agctgtccga gctcttcgag cgcgccggca gctagcgacg ccggccccgc accagatggc
     5041 gcggggccgg aaattcgctc gacacggctc tacggcacga gcgccatgtt cggctcgccc
     5101 tgcacgttga gcccgttgct cgccctcacg cagcggccgc cgaccggcga accgccgttg
     5161 taggccagtc gcaggcagtt gcgcattgcc ttctgacccc agtagctggc atgcatgctc
     5221 tcctggatct ggtacggacc gaacaccgtc gtgacggtcc tgacctgcgc cacccactcg
     5281 gtgttgtcga ccgcgccggg actggtccag ttcgcgatcc cctcctcctc gagcaggccg
     5341 acggtgttct cgcacagccg ccgcccgttg agcgccgtct gcatgtcgat caccgccgtg
     5401 ttcgtcaggc cgctcgtcgc gagcgcgttg cgcatcgtgt tgttgagcgc gttgacgacc
     5461 gtgtcgttgg cccagtcggc gtcgcggttc caggtgccgc aaccgccgat gctctggcgc
     5521 gtccagcccg actccgggta gcggatctgg gcgccacgcg gcagcggcga ccagtaggtc
     5581 tggccgagga tcttgtactg cgacggcgag tagccggcat tgctcatcgc ctgggcgacg
     5641 cgcagcagcg cgtcccgcac ctcggtcgtc cgcgcggcct gcgccgacgg ggtgaacttc
     5701 gagaccatgt cggagtcgtc ggagcagtag ttcttccacc acgacggcga agtcagccag
     5761 ttggtgatgc agcgctggac gatgtcggcg aagccgtagt tattggcgcc gatcatcacc
     5821 acgaccgcct tgacgttgtg cgtcgccgcg tactcctgca gccgcagcgc ctggcccttg
     5881 cggccagagg cgtcgctgta gaagtcgatc ccgggcttga agtcctcgcc cgaggccgtc
     5941 cccgacgtgt acgtccgcgc cccggagcac gccaggttgg cgctcgccac gccaccgccg
     6001 atgtgcgcct gcgcgctctg cgaccggtga cagcccggga tcgactccgc tcccggcacg
     6061 tcgtggtacg cggccggtcc gagcgcgtcg acgttcgacg aagacccgtt cgtgttgccg
     6121 gcccagcgac cggcctcgcc cgagatcgcc gagtcgccga gcgtcacgac cgtcggcgtg
     6181 ccgactcccg gcccgtcggc cgttgccgcg gccggcagcg ccgccgccag cgcggccacc
     6241 gcaaagccga agagcgcacg ctttccgcgc gcgcggcttg cctccaccat ctcgaacccc
     6301 tcccgttggt tgcgcgccga accccttcga cgcgcggcga gtatctcagg gatgagaaac
     6361 actgacgtct ctgag
//
LOCUS       DATABV010000093        14417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1955319, whole genome shotgun sequence.
ACCESSION   DATABV010000093 DATABV010000000
VERSION     DATABV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14417
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955319"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VHF88_03850"
     CDS             <1..816
                     /locus_tag="VHF88_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2160935.1"
                     /translation="AAGVETTALTGVGVGSPGEIDAAAGTVTQARNLPDWEGSYPLAA
                     ELSDLLGAPVALGNDVTVATAAEAELGAGRGFGSLLGVFWGTGVGGGIVLDGRPWVGR
                     GAAGELGHVVVRIGGRRCPCGRRGCLEAYAGRGSMEARARELAGEGRATKLFELMEKH
                     GRDRLTSGIWARALERDDALAHEIVGEAVEALGAGVASAVNLLDVEAVILGGGLGTKL
                     GEPYREHVKAAMLPHLFGDDDPPAVRLAELGDLGGAIGAALLARPQGGGAPNG"
     gene            809..1804
                     /locus_tag="VHF88_03855"
     CDS             809..1804
                     /locus_tag="VHF88_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2160936.1"
                     /translation="MAEPAAAGPLLGIDVGGTKVAAAVVDGLRIVERVERPTETASTE
                     ALLDEIGAVAREAIAKAGGVKPRAAGAGIPSQIDAASGTALASVNIPLSGVPVRAELG
                     KRLGVPVFVDNDANCAALAEARLVPDPPADELVMLTLGTGVGGGIVIDGRIFRGATGL
                     GAELGHIVVDGREQAPAEPGAFPRPGSLEWHCSGRGLEREATGCAERHPDGELGRRLA
                     ARGRVSGRDAVAAARAGDERALDLMAAYGRWLGIGIATVVNVFEPQRVVIGGGISEAA
                     ELFLDVAVAEAARWALPALWERTTVGLARGGADAGAIGAALLAAHELERKGHTAR"
     gene            1836..3875
                     /gene="tkt"
                     /locus_tag="VHF88_03860"
     CDS             1836..3875
                     /gene="tkt"
                     /locus_tag="VHF88_03860"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2160937.1"
                     /translation="MTATTIDREALDKLCVDTIRTLSIDMVQKANSGHPGAPMALAPV
                     AYQLYTRTLKHNPANPSWFDRDRFVLSAGHASALLYSILHLTGYGISMDDLKRFRQIL
                     SPTAGHPEHEAEGTPGVEVTTGPLGQGVANAVGFALAERMLAARYNRPGHTVIDHHTY
                     AIASDGDLQEGISAEASSLAGHLGLGRLIVFYDSNHIQLAGSTEMAFSENVHLRYEAY
                     GWHTIEMGDDLTLEGIAAAIEEAKAVEDRPSLIVLHTHIGYGSPNKQDTHSAHGSPLG
                     EDEVRLTKEAYGWDPDAHFLVPDEAREHFLAAVDRGRDAEADWQRRLDSYRDEHPDVC
                     AELELVMAGKLPNGWDAEVPRFDAGGDPIATRKASAKVIQWAAAQVPQLVSGSADLEP
                     STLTEIEDGGSVARGESEGRNVHYGVREHAMGGIVNGLNLHMVRAFGSTFFNFLDYQK
                     ASLRLAALMRLPALYLFTHDSIGLGEDGPTHQPIEQLAHLRATPFVYAVRPADANETA
                     LAWRFALREERGPCVLVLTRQGLPIIDPELIPDDAIERGAYVLRDAAAGAEPRLILIG
                     TGSEVALCLEAAERLEAGGIATRVVSMPCFDSFGEQDEAYRDSVLPPSCRARVSVEAG
                     ATLGWDRWIGEAGEAVGMTGFGASGPAKDLFEHFGFTPENVADTGRRVVERVEAG"
     gene            3899..6625
                     /locus_tag="VHF88_03865"
     CDS             3899..6625
                     /locus_tag="VHF88_03865"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX2160938.1"
                     /translation="MDATTAQNERLAKLTEAGVSVWLDQIRRSLIESGELARLRDELS
                     LRGETSNPAIFEKAILGSDEYDEDIARLAREGKSATDIYLDIAVRDVQAACDVLRPVW
                     EDAGGADGFVSLEVEPSLAHDTDATLASARELWRRVDRPNVMIKIPGTEEGIPAIEQA
                     TADGINVNVTLLFKVESYEAIAEAYVRGMERRLEAGESLDVHSVASFFVSRVDTEVDK
                     RLAKLGREDLRGQAAIANAQAAYKSFKRIFHGERYAKLRAAGAPLQRPLWASTGVKDP
                     AYAETKYVDGLVAPTTVNTMPMQTLLAVAERSEIDPGSAERDPDPVLDALKEAGIDMP
                     DVTDTLLRDGIDKFVDPFDKLITGIESIREAALTGRPKAIESSLPEDLEGPIADRAKL
                     AEHENVARRLWAKDESLWGGPGIPEIADRLGWLTVSDELLEQVGDLREFVEGVRADGF
                     TDAVLLGMGGSSLGPEVVRRSFGEQEGGLRLHVLDTTDASAILALERSLDLAKTFFLV
                     SSKSGGTVETLSHMKHFYERSGGNGAQFAAVTDPGSPLVPEARQRGFRRVFENNPNIG
                     GRYSVLSHFGMVPAALMGVDVEALLGSAKEAEAACQSFDSTASNAGLWLGAAIGEAAL
                     RGRDKLTFVVSPRIASFGLWVEQLVAESTGKEGRGILPVAGEPLGAPESYGDDRLFVY
                     LRDADEPDADLEAGMQALREAGQPVITVATSGPERLGRVFFFAEFAVAVAGWVLGINP
                     FDQPNVQEAKDKTNAVLAADRPPVVEDADDDALRGLLDQARPPHYLAILGYLQPTAEF
                     DAAVDELRTVVRDATKATTTFGYGPRFQHSTGQYHKGGPKTGLFLQLIHDGDEDVEVP
                     EAGYTFDELKNGAATGDVLTLRDHGLPAERVRLTGDPVAALRELTAKIEAML"
     gene            6631..8157
                     /gene="zwf"
                     /locus_tag="VHF88_03870"
     CDS             6631..8157
                     /gene="zwf"
                     /locus_tag="VHF88_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX2160939.1"
                     /translation="MLVEPAVNPLAEGLERLPIRPTTLTIFGATGDLSRRKLLPALYN
                     LAHEGALPERFNLVGSSRSDMPHGDFREMARDAIRQYSRRKPDETVLAALIDHARFVP
                     GSFDDPKVYDEIAKVERELDEDAGIVFNRVFYLSTAPAFFPVIVAKLGEHGLNAKEGA
                     EVRVVIEKPIGTNLAEAQELNREVLDVLDERQAFRIDHYLGKETVQNMLAFRFANGLF
                     EPIWNRNFVDHVQITAAEDIGIGSRAGYYDKAGALRDLVQNHMLQLLTLLCMEPPVSF
                     SADAVRDEKVKVLHAIHPPDPAHVDAMAVRGQYTAGVVGGDRVPGYLDEEGVPSDSTT
                     PTFVALRLEVDNWRWAGVPIYLRTGKRLARKVTEIAVTLKPVPHLAFDEQGSVGVQPN
                     QIILGVQPNEGVTISLGAKIPGSRMRIRPVNMEFLYGTSFMSESPEAYERLITDAMRG
                     DATLFTRNDEVEAQWAICDPILAGWAADGEPVVQYEAGSAGPPEADRLLRPGERWRAI
                     "
     gene            8154..9227
                     /locus_tag="VHF88_03875"
     CDS             8154..9227
                     /locus_tag="VHF88_03875"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX2160940.1"
                     /translation="MSESGTVWSARDTTPSAVEEALRDLLKQRYEAGEHYAPARVLNL
                     VVIADREWRGEIQNRLEKVGRYHASRTIICAVDPGRTEIDAVASVTADEDDIAAGRLV
                     VAREEVVVDVGPDQLAHLDRVVDPLLVSDLATLVWSPHGHDEAVDSLLRLAQIVLIDS
                     ISALDVGDAVARAVALMDRAYVVDLAWLRSTPWRERVAATFDPPQWQPELARISAVTV
                     RHQRDSAAAAVLLLGWLARRLGWQPGPICAPDASGHCEGVARGDDGEVRLTLDRDETM
                     PIPGLAGVTIETRSGLAISLDRGPGGLSAWRRTPEGHESRWTVTGASRGESGILGEGI
                     RQALLRDKGYRQALIAAEAMVCS"
     gene            9224..9937
                     /gene="pgl"
                     /locus_tag="VHF88_03880"
     CDS             9224..9937
                     /gene="pgl"
                     /locus_tag="VHF88_03880"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX2160941.1"
                     /translation="MSREIAVVDDPAAAAAERIAAVVRRGGQIALAGGSTPRRAYELL
                     AAMDLDWSRCELWLGDDRCVPADHEHSNYKMVSEELVDRLPVCHRPSGWHRIPGELGP
                     RAAADAFEADLRAAFPTDAHLPVFDLVLLGIGPDAHTASLFPGEAALDERERWAVGVE
                     TPGMAPLVPRVTLTLPVLDAAREVVFLVAGADKAEAVARAFGDAPGPDAPASLVRPSN
                     GALTVLLDRPAATRLGAGD"
     gene            complement(10055..10261)
                     /locus_tag="VHF88_03885"
     CDS             complement(10055..10261)
                     /locus_tag="VHF88_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160942.1"
                     /translation="MIGAEIICTDESCAVALDVSVDTLDELELLACDDCGCTIQILAV
                     WDVTELRPAAPVTVLRRTPPPLAA"
     gene            complement(10258..11778)
                     /locus_tag="VHF88_03890"
     CDS             complement(10258..11778)
                     /locus_tag="VHF88_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2160943.1"
                     /translation="MSDPPDEPAAPAQPAASSAADQPTAPALPARRLHPAGAILSALD
                     DIRNALFSVVVLIVLGSRSANFGTIAVLGGLVGAAVAAWVGYMRWRAETYEIADGAIR
                     HRSGIISPDETVVPLARVHSIDTSQGPVQRIFGIYELHVQTAGGGEKGEIVLRAVSPA
                     ETARLRAAAGLPEPTTTDLPEWRLSPRGLLLVGLTAPQLGMLLPLVGGAAALIDDVLG
                     EGTRNSLADRLPTDTGGILLLIAAAIAAAWLLSFLGSLVTFAGFSVVRDDDQLRIRRG
                     FLQRRATSVPLRRIHAVSVAEGPLRRPLGLCSVRLETAGYGRDPAAARTLFPLLPIGQ
                     LDAQLATLVPALASGSPTSPQAPAATAPHTPQFQRPPHRAARRYIAPPTLTILLVTVA
                     ATLAWPSVWPAIPILTALAALAGLTSYRSAGYRIDADRLLLRRGTLTRRTLVARPNHL
                     QDHSLSQNAFQARAQLANLSITVASGTNSTVAHLDSTAATTLFNRLRPTSPSTSQA"
     gene            complement(11771..12256)
                     /locus_tag="VHF88_03895"
     CDS             complement(11771..12256)
                     /locus_tag="VHF88_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2160944.1"
                     /translation="MEGGTPEPTQRLPGQARLLFAGRTLGWAVPLVVGSFIADSVLTE
                     SGAPDSIGLGLTLAALALALLGLVLEPQRRWRNWRYELRDEEIDLRRGVLVITRTLIP
                     TIRVQHVDTQRTWLSDQLGLRSVRIHTAGGSHTIPALRPDEAAAIRDRIAELAQRPDD
                     V"
     gene            complement(12270..13187)
                     /locus_tag="VHF88_03900"
     CDS             complement(12270..13187)
                     /locus_tag="VHF88_03900"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX2160945.1"
                     /translation="MPPPAASSQSAVAAAPRARSRTRQLVADYVEMTKPKVQSLLIFT
                     TVTTMYVAGDPSIGLVALTCLGGYLSAGGAGAVNHWYDRDIDAQMGRTADRPVASGRV
                     APNAALVYGIALAALACVVLAVGVNPLAAALSLSGFLGYTLVYTVWLKRTTPQNIVIG
                     GAAGAVPPLVGWAATTGSLSWTALYLFAIVFYWTPPHFWALSLLMKDEYAKVGVPMMP
                     VVRGEQETRRQIVLYSLLLYAITQLPFCAGGFGVPYLVGSVALGGVFIAFAIQLYRNP
                     SRPAALRLYLYSLLYLALLFGAMVVDARL"
     gene            complement(13191..14225)
                     /locus_tag="VHF88_03905"
     CDS             complement(13191..14225)
                     /locus_tag="VHF88_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX2160946.1"
                     /translation="MTPSAAGLSEGAAGPDRTYRRLALATIATTFVLILIGGIVRVSG
                     SGLGCGPADSGFHGWPFCNGALIPGLDVNTIIEYTHRAVAGIVGLMMIALAVLAWRRY
                     RANRALVRATAAAALLVIAQGLLGAATVENDLDETLVAAHLGLAMALLALLIYIWRAS
                     QPETLGAPPADGGRGFRPLALVSSGLAFCTIVAGGYMAGTQNYGRADYQLGDGAHHAC
                     GKEFPTCNGDFLPFGVTRLVDIHLTHRVFVYLTTIAVVTLIVLALRRRPSPGVVLSAK
                     VAAAVLATQLLVGALNVWLDEYEALIVLHLALGTLLWGTLVGLAFQLFRIPEPVESRS
                     GASNREALTA"
     gene            complement(14222..>14417)
                     /locus_tag="VHF88_03910"
     CDS             complement(14222..>14417)
                     /locus_tag="VHF88_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160947.1"
                     /translation="GKVAGNDPWKGNTLEWFVQSPPPVNNFDVVPRVRSVEPMKDIRR
                     EVARSGEAVGSVAQPVASGT"
BASE COUNT         2087 a         4894 c         5537 g         1899 t
ORIGIN      
        1 gccgcggggg ttgagacgac ggcgctcacg ggcgtcgggg tcgggtcgcc cggcgagatc
       61 gacgccgccg cgggaaccgt tacccaggcg cgcaacctgc cggattggga gggctcgtat
      121 ccgctcgccg cggagctgag cgacctgctc ggagcgcccg tcgccctcgg caacgacgtc
      181 acggtcgcga ccgcggccga ggccgagctc ggcgcgggcc gcggcttcgg gtcgctgctc
      241 ggcgtcttct gggggacggg ggtcggcgga gggatcgtcc tcgacgggcg gccgtgggtc
      301 ggtcgcgggg cggctggcga gctcggccac gtcgtcgtgc ggatcggcgg ccgccgctgt
      361 ccgtgcgggc gccgcggctg cctcgaggcc tatgccgggc gcggctcgat ggaggcgcgt
      421 gcgcgcgagc tggccggcga agggcgtgcg acgaagctct tcgagctgat ggagaagcac
      481 gggcgcgacc gcctgacgag cgggatctgg gcacgggcgc tcgagcgcga cgatgcgctg
      541 gcgcacgaga tcgtcggcga ggctgtcgag gcgctcggcg ccggtgtggc gtcggcggtc
      601 aacctgctcg acgtggaggc ggtgatcctc ggcggcgggc tcggcaccaa gctcggcgag
      661 ccctaccgcg agcacgtcaa ggcggcgatg ctgccgcact tgttcggcga cgacgacccg
      721 cccgcggtcc gcctggccga gctcggcgac ctcggcgggg cgatcggcgc ggcgctgctc
      781 gcccgcccgc aaggcggcgg agcgccgaat ggctgagccc gcggcggccg ggccgctcct
      841 cgggatcgac gtcggcggca cgaaggtcgc ggcggccgtc gtcgacgggc tgcggatcgt
      901 cgagcgggtc gagcggccga ccgagaccgc gagtaccgag gcgctgctcg acgagattgg
      961 ggccgtggct cgtgaggcca tcgcgaaggc gggcggggtc aagccgcgcg ccgccggagc
     1021 gggaattccc tcgcagatcg acgccgcgag cggcaccgcg ctggccagtg tgaacatccc
     1081 gctgagcggc gttccggtgc gcgcggagct gggcaagcgg ttgggagtgc cggtcttcgt
     1141 cgacaacgac gccaactgcg ccgccctcgc ggaggcccgg ctcgttcccg atccgcccgc
     1201 cgacgagttg gtgatgctga cgctcgggac cggcgtcggc ggcgggatcg tgatcgacgg
     1261 gcggatcttc cgcggtgcga ctgggctggg cgcggagctc ggccacatcg tggtcgacgg
     1321 gcgcgagcag gcgcccgccg agccgggggc cttcccgcgc cccggatcgc tggagtggca
     1381 ctgcagcgga cgcgggctgg agcgcgaggc gaccggctgc gccgagcgcc accccgacgg
     1441 tgagctcggc cgccgcctcg ccgcgcgcgg ccgcgtctcc ggtcgcgacg ccgtcgccgc
     1501 cgcgcgtgcc ggcgacgagc gcgcactcga cctgatggcc gcgtacggcc gctggctggg
     1561 aatcggtatc gccacggtgg tgaacgtctt tgagccgcag cgcgtcgtga tcggcggtgg
     1621 gatctccgag gcggccgagc tgttcctcga cgtcgccgtc gccgaggccg cgcgctgggc
     1681 gctgcccgcg ctctgggagc ggacgaccgt cggcctcgcg cgcggcggtg ccgacgcggg
     1741 cgcgatcggc gccgcgctgc tcgccgctca cgagctcgag cgcaagggac atactgcgag
     1801 gtgaacgtgc aaagagcgag cgacagggga gttggatgac cgccacgacg atcgaccgcg
     1861 aggctctcga caagctctgc gtcgacacga tccggaccct gtcgatcgac atggtccaga
     1921 aggcgaactc cggtcatccc ggcgcgccga tggcgctcgc gcccgtcgcc taccagctct
     1981 acacgcgcac gctcaagcac aaccccgcga acccgagctg gttcgaccgc gaccgcttcg
     2041 tgctctcagc gggtcacgcc tccgcgctgc tctactcgat cctgcacctg accggctacg
     2101 ggatctcgat ggacgacctg aagcggttcc gccagatcct cagcccgacc gccggccacc
     2161 ccgagcacga ggcagagggc acgcccggcg tcgaggtgac caccggcccg ctcggccagg
     2221 gcgtggcgaa cgccgtcggc ttcgcgctcg ccgagcggat gctcgccgcg cgctacaacc
     2281 ggcccggcca cacggtgatc gaccaccaca cctacgcgat cgccagcgat ggcgacctcc
     2341 aggagggcat ctcggcggag gcgagctcgc tcgccggaca tctcggcctc gggcgactga
     2401 tcgtcttcta cgacagcaac cacatccagc tcgccggctc gaccgagatg gcgttcagcg
     2461 agaacgtcca cctgcgctac gaggcctacg ggtggcacac gatcgagatg ggcgatgacc
     2521 tcacgctcga gggcatcgcg gcggcgatcg aggaggcgaa ggcggtcgag gaccgtccat
     2581 cgctgatcgt cctgcacacc cacatcggct acggctcgcc gaacaagcag gacacccact
     2641 cggcgcacgg ctcgccgctc ggcgaggacg aggtgcggct gacgaaggag gcctacggct
     2701 gggaccccga cgcccacttc ctcgtcccgg acgaggcgcg cgagcacttc ctcgcggcgg
     2761 tcgaccgcgg tcgcgacgcc gaggcggact ggcagcgccg gctcgactcc taccgcgacg
     2821 agcacccaga cgtctgcgcg gagctggagc tagtgatggc gggcaagctg cccaacggct
     2881 gggacgccga ggtcccgcgc ttcgacgcgg gcggtgaccc gatcgcgacg cgcaaggcat
     2941 cggcgaaggt gatccagtgg gcggcggcgc aggtgccgca gctggtcagc gggtcggcgg
     3001 acctcgagcc ctcgacactg accgagatcg aggacggcgg ctcggtcgcg cgcggcgagt
     3061 ccgagggccg caacgtccac tacggcgtcc gcgagcacgc gatgggcggg atcgtcaacg
     3121 ggctcaacct gcacatggtg cgcgccttcg gctcgacctt cttcaacttc ctcgactacc
     3181 agaaggcgtc gctgcgactg gcggccctga tgcggctgcc ggcgctctac ctcttcacgc
     3241 acgactcgat cggcctcggc gaggacgggc cgacccacca gccgatcgag cagctcgccc
     3301 acctgcgtgc gacgccgttc gtctacgccg tccggccggc cgacgccaac gagacggcgc
     3361 tcgcctggcg cttcgcgctg cgcgaggagc gcgggccgtg cgtgctggtg ctgacccgcc
     3421 aggggctgcc gatcatcgac cccgagctga tccccgacga cgctatcgag cgcggcgcct
     3481 acgtgctgcg cgacgccgcc gcaggcgcgg agccgcggct gatcctgatc ggcaccggct
     3541 ccgaggtcgc gctctgcctc gaggccgccg agcggctcga ggccggcggg atcgcgacgc
     3601 gcgtcgtcag catgccctgc ttcgacagct tcggcgagca ggacgaggcc taccgcgaca
     3661 gcgtgctgcc gccgagctgc cgcgcgcgcg tctcggtcga ggcgggggcg accctcggct
     3721 gggaccgctg gatcggcgag gccggcgagg ccgtcgggat gaccggtttc ggcgcctccg
     3781 gaccggctaa ggacctgttc gagcacttcg gcttcacgcc cgagaacgtc gccgataccg
     3841 gccggcgggt cgtcgagcgc gtcgaggccg gctagagaga cccaccgagg agaccgacat
     3901 ggacgccacc actgcacaga acgagcggct cgcgaagctc accgaggctg gcgtcagcgt
     3961 ctggctcgac cagatccgtc gctcgctgat cgagtcgggc gagctcgcac ggctgcgcga
     4021 cgagctgtca ctgcgtggcg agacgtcgaa cccggcgatc ttcgagaagg cgatcctcgg
     4081 ctccgacgag tacgacgagg acatcgcgcg gctcgcccgc gagggcaaga gcgcgaccga
     4141 catctacctc gacatcgcgg tgcgcgacgt ccaggcggcg tgcgacgtgc tgcgcccggt
     4201 ctgggaggac gcgggcggcg ccgacggatt cgtgtcgctc gaggtcgagc cgtcgctcgc
     4261 ccacgacacc gacgcgacgc tcgcgtccgc acgcgagctc tggcggcggg tcgaccggcc
     4321 gaacgtgatg atcaagattc cgggcaccga ggagggcatc ccggcgatcg agcaggcgac
     4381 cgccgacggc atcaacgtca acgtgacgct gctgttcaag gtcgagtcct acgaggcgat
     4441 cgccgaggcc tacgtgcgcg gcatggagcg ccggctggag gccggagagt cgcttgacgt
     4501 gcactcggtc gcgagcttct tcgtctcgcg cgtcgacacc gaggtcgaca agcggctagc
     4561 caagctcggc cgcgaggacc tgcgtggcca ggcggcgatc gccaacgccc aggcggccta
     4621 caagagcttc aagcggatct tccacggcga gcgctacgcg aagctgcgcg cggccggggc
     4681 accgctgcag cggccgttgt gggcctcgac gggagtcaag gaccccgcct acgccgagac
     4741 caagtacgtc gacgggctgg tcgcaccgac gaccgtcaac acgatgccga tgcagacgct
     4801 gctcgcggtc gccgagcgct cggagatcga tcccggctcg gccgagcgcg acccggatcc
     4861 ggtgcttgac gcgctcaagg aggccgggat cgacatgccc gacgtcaccg acacgctgct
     4921 gcgcgacggc atcgacaagt tcgtcgaccc tttcgacaag ctgatcaccg gcatcgagtc
     4981 gatccgcgag gccgcgctga ccggccgccc gaaggcgatc gagtcgtcgc tgcccgagga
     5041 cctcgagggg ccgatcgccg accgcgcgaa gctcgccgag cacgagaacg tcgcgcgtcg
     5101 actgtgggcc aaggatgagt cgctctgggg cggcccgggc attccggaga tcgctgaccg
     5161 gctcggctgg ctgaccgtct ccgacgagct gctcgagcag gtcggcgacc tgcgcgagtt
     5221 cgtcgagggg gtcagggccg acgggttcac cgacgccgtg ctgctcggga tgggcggctc
     5281 cagcctcggg cccgaggtgg tccggcgcag cttcggcgag caagagggcg gcctgcgcct
     5341 gcacgtgctc gacacgaccg acgcaagcgc gatcctcgcc ctcgagcgct cgctcgacct
     5401 cgccaagacg ttcttcctcg tctcgtcgaa gtccggcggg acggtcgaga cgctgtcgca
     5461 catgaagcac ttctacgagc gcagcggcgg caacggcgcc cagttcgcgg ccgtcaccga
     5521 ccccggcagc ccgctcgtcc ccgaggcgcg gcagcgcggc ttcaggcggg tcttcgagaa
     5581 caacccgaac atcggcgggc gctactcggt gctgtcgcac ttcgggatgg ttccggcggc
     5641 actgatgggc gtcgacgtcg aggcgctgct cggctccgcc aaggaggccg aggcggcgtg
     5701 tcagagcttc gactcgacgg cgtcgaacgc cggcctgtgg ctcggcgcgg cgatcggcga
     5761 ggcggcgctg cgcggtcgcg acaagctgac cttcgtcgtg tcgccgcgga tcgcgagctt
     5821 cggcctctgg gtcgagcagc tcgtcgccga gtcgaccggc aaggaggggc gcgggatcct
     5881 gccggtcgcc ggcgagccgc tcggcgcgcc ggagagctac ggcgacgacc gcctgttcgt
     5941 ctacctgcgc gacgccgacg agcccgacgc cgacctcgag gccgggatgc aggcgctgcg
     6001 cgaggcgggc cagcccgtga tcaccgtcgc gaccagcggg ccagagcgcc tgggccgggt
     6061 cttcttcttc gccgagttcg ccgtcgccgt cgcgggctgg gtgctcggca tcaacccgtt
     6121 cgaccagccg aacgtgcagg aggccaagga caagacgaac gcggtgctgg ccgcagatcg
     6181 gccgccggtc gtcgaggacg ccgacgacga cgcgctgcgc ggcttgctcg accaggcgcg
     6241 tcccccgcac tacctggcga tcctcggcta cctgcagccg accgccgagt tcgacgccgc
     6301 ggtcgacgag ctgcggacgg tcgtccgtga cgccaccaag gcgacgacca ccttcggcta
     6361 cggcccgcgc ttccagcact cgaccggcca gtaccacaag ggcggtccga agaccggcct
     6421 cttcctacag ctgatccacg acggcgacga ggacgtcgag gtgccggagg cgggctacac
     6481 cttcgacgag ctcaagaacg gggccgccac cggcgacgtg ctcacgctgc gcgaccacgg
     6541 gcttcccgcc gagcgcgtcc gcctgaccgg agacccggtc gccgccctgc gcgagctgac
     6601 cgccaagatc gaggcgatgc tgtgaccgac atgctggtcg agcctgcggt caacccgctc
     6661 gcggagggcc tcgagcggct gccgatccgg ccgacgacgc tgaccatctt cggcgccacc
     6721 ggcgacctgt cgcggcgcaa gctgctgccg gcgctctaca acctcgccca cgagggggcg
     6781 ctgcccgagc ggttcaacct cgtcgggtcc tcgcgcagcg acatgccgca cggcgacttc
     6841 cgcgagatgg cgcgcgacgc gatccgccag tactcgcgcc gcaagcccga cgagaccgtg
     6901 ctcgcggcgc tgatcgacca cgcccgcttc gttcccggca gcttcgacga cccgaaggtc
     6961 tacgacgaga tcgcgaaggt cgagcgagag ctcgacgagg acgcgggaat cgtcttcaac
     7021 cgcgtcttct acctctcgac cgcgccggcg ttcttcccgg tcatcgtcgc caagctcggc
     7081 gagcacggcc tcaacgcgaa ggagggcgcg gaggtccgcg tcgtgatcga gaagccgatc
     7141 ggcaccaacc tcgccgaggc ccaagagctc aaccgcgaag tgctcgacgt gctcgacgag
     7201 cgccaggcgt tccggatcga ccactacctg ggcaaggaga ccgtccagaa catgctggcg
     7261 ttccggttcg cgaacggcct gttcgagccg atctggaacc gcaacttcgt cgaccacgtc
     7321 cagatcaccg ccgccgagga catcggcatc ggcagtcgcg cgggctacta cgacaaggcc
     7381 ggcgcgctgc gcgatctcgt ccagaaccac atgctgcagc tgctgacgct gctctgcatg
     7441 gagccgccgg tctcgttctc cgccgacgcg gtgcgcgacg agaaggtcaa ggtgctccat
     7501 gcgatccacc cgcccgatcc cgcgcacgtc gacgcgatgg ccgtacgcgg ccagtacacc
     7561 gccggcgtgg tcggcggcga ccgcgtcccc ggctacctcg acgaggaggg cgtgccctcc
     7621 gactcgacca cgccgacctt cgtggcgctg cggctcgagg tcgacaactg gcgctgggcc
     7681 ggcgtgccga tctacctgcg caccggcaag cggctggcgc gcaaggtcac cgagatcgcc
     7741 gtgacgctga agccggtgcc gcacctcgcc ttcgacgagc aggggtcggt cggcgtccag
     7801 ccgaaccaga tcatcctcgg cgtccagccg aacgagggtg tgacgatctc gctcggcgcc
     7861 aagatccccg gcagccggat gcggatccgc ccggtcaaca tggagttcct ctacgggacg
     7921 tcgttcatgt ccgagtcgcc cgaggcctac gagcggctga tcaccgacgc gatgcgcggc
     7981 gacgcgaccc tgttcacgcg caacgacgag gtcgaggcgc agtgggcgat ctgcgatccg
     8041 atcctcgccg gctgggcggc cgacggcgag cccgtcgtgc agtacgaggc gggcagcgct
     8101 gggccgcccg aggccgatcg cctgctgcgg cccggggagc gctggcgggc gatatgagcg
     8161 agagcggcac cgtctggtcc gcccgcgaca cgacgccgtc cgccgtcgag gaggcgctgc
     8221 gcgacctgct caagcagcgc tacgaggccg gtgagcacta cgccccggcg cgcgtactca
     8281 acctcgtcgt gatcgccgac cgcgagtggc gcggcgagat ccagaaccgg ctcgagaagg
     8341 tcggccgcta ccacgcctcg cgcacgatca tctgcgccgt cgacccggga cggaccgaga
     8401 tcgacgccgt cgcgtcggtc accgccgacg aggacgacat cgccgccggg cggctcgtcg
     8461 tcgcccgcga ggaggtcgtc gtcgacgtcg gtcccgacca gctcgcccac ctcgaccggg
     8521 tcgtcgaccc gctgctcgtg tccgacctcg cgacgctcgt ctggtcgccc cacggtcacg
     8581 acgaggccgt cgactcgctg ctgcggctcg cccagatcgt cctgatcgac tcgatcagcg
     8641 cgctcgacgt cggcgacgcg gtcgcccgcg ccgtcgcgct gatggatcgc gcctacgtcg
     8701 tcgatctcgc ctggctgcgc tcgacgccgt ggcgcgagcg cgttgcggcg accttcgacc
     8761 cgccgcagtg gcagccggag ctcgcgcgga tcagcgccgt cacggtccgc caccagcgcg
     8821 actctgcggc cgcggccgtg ctgctgctcg gctggctggc gcggcggctc ggctggcagc
     8881 ccggcccgat ctgcgcgcct gacgccagcg ggcactgcga gggcgtcgcc cggggcgacg
     8941 acggcgaggt gcggctcacg ctcgaccgcg acgagacgat gccgatcccc ggcctcgccg
     9001 gcgtcacgat cgagacgcgc tccgggctgg cgatctcgct cgaccgcggg ccgggcgggc
     9061 tgagcgcgtg gcgccgcacg cccgaaggcc acgagtcgcg ctggactgtc accggcgcct
     9121 cacgcggcga gtcggggatc ctcggcgagg ggatccgcca ggcgctgctg cgcgacaagg
     9181 gctaccgcca ggcgctgatc gccgccgagg cgatggtctg ctcgtgagcc gcgagatcgc
     9241 ggtcgtcgac gatccggcgg ctgccgccgc agagcggatc gcggcggtgg tgcggcgtgg
     9301 cgggcagatc gcgctggccg gcggctcgac gccgcggcgg gcgtacgagc tgctggccgc
     9361 gatggacctc gactggagcc gctgcgagct gtggctcggc gacgaccgct gcgtgccggc
     9421 cgaccacgag cactcgaact acaagatggt cagcgaggag ctcgtcgacc ggcttccggt
     9481 ctgccaccgg ccgtccggct ggcaccggat ccccggggag ctggggccgc gggcggcggc
     9541 cgatgcgttc gaggcggatc tgcgcgccgc gttcccgacg gatgcccact tgccggtgtt
     9601 cgatttggtg ctgttgggga tcggtccgga cgcccatacg gcgtcgctgt ttccgggtga
     9661 ggctgctctg gatgagcggg agcggtgggc ggtgggggtc gagaccccgg ggatggcgcc
     9721 gctcgtgccg cgggtgacgc tgacgttgcc ggtgttggat gcggcgcgtg aggtggtgtt
     9781 cctggtcgcg ggggcggaca aggcggaggc cgtggctcgc gcgttcggcg acgcgcccgg
     9841 gccggacgcg ccggcgagct tggtgcggcc gtcgaacggg gcgctgaccg tgctgctcga
     9901 ccgaccggca gcaacgcgtc tcggagctgg cgactgacga actaggggct ggcgttggtg
     9961 gccgcgggtc ctgtctccga ttttggttta ctgctcgttc ctgcgcgcgc aaacgcaaaa
    10021 tcgtcgccac ccgcggatgg ggcgtctccg actactaggc ggcgagcggg ggtggtgtcc
    10081 tacgtaggac ggtgaccggc gcggcggggc ggagctctgt gacgtcccag acggcgagga
    10141 tttggatggt gcagccgcag tcgtcgcagg cgagtagctc gagctcgtca agcgtgtcga
    10201 ccgagacgtc gagggccacg gcgcaggact cgtcggtgca gatgatctcg gctccgatca
    10261 cgcttgtgat gttgagggcg acgtcggacg gaggcggttg aagagggtgg ttgcggctgt
    10321 gctgtcgagg tgggcgacgg tggagttggt gccggaggcg acggtgatgg agaggttggc
    10381 gagctgggct cgggcttgga aggcgttctg gctgaggctg tggtcttgga ggtggttggg
    10441 gcgggcgacg agggtgcggc gggtgagcgt gccgcggcgg aggaggagac ggtcggcgtc
    10501 gatgcggtag ccggcggagc ggtagctggt gaggccggcg agggccgcta gggcggtgag
    10561 gatggggatt gcgggccaga cgctgggcca ggcgagggtg gcggcgaccg tgaccagcag
    10621 gatggtgagg gtcgggggcg cgatgtagcg gcgggcggcg cggtggggtg ggcgctggaa
    10681 ctggggcgtg tgcggggcgg tcgcggcagg ggcttgtggg gacgtcggcg atccactggc
    10741 gagggcgggg acgagggtgg cgagctgggc gtcgagctgg ccgatcggaa gcagggggaa
    10801 gagggtgcgg gcggcggctg ggtcgcggcc gtagccggct gtctcgaggc ggacgctgca
    10861 gaggccgagg gggcggcgga gaggcccctc ggcgaccgag acggcgtgga tgcgacggag
    10921 ggggacgctg gtggcgcggc gctggaggaa gccgcggcgg atgcgcagct ggtcgtcgtc
    10981 gcggacgacg gagaaaccgg cgaaggtgac gagcgagccg aggaaggaga gcagccaggc
    11041 ggcggcgatg gcggcggcga tcaggagcag gatgccgccg gtgtcggtcg ggaggcggtc
    11101 ggcgagcgag ttgcgggtgc cctcgccgag cacgtcgtcg atcagcgcgg cggcgccgcc
    11161 gacgagcggg agcagcatgc cgagctgggg tgctgtgagc ccgacgagta ggaggccgcg
    11221 gggggagagg cgccactcgg gcagatcggt cgtcgtcggc tcggggaggc cggcggcggc
    11281 acgcaggcgg gcggtctcgg ccggactaac ggcgcgcagg acgatctcgc ccttctcgcc
    11341 gcctccggcg gtctggacgt gaagctcgta gatcccgaag atgcgctgga cggggccctg
    11401 gctggtgtcg atcgagtgga cgcgggcgag cggcacgacg gtctcgtccg gcgagatgat
    11461 gccgctgcgg tggcggatgg cgccgtcggc gatctcgtag gtctcggcgc gccagcgcat
    11521 gtagccgacc caggcggcga ccgctgcgcc gaccagaccg ccgagcacgg cgatcgtgcc
    11581 gaagttggcg gagcgcgagc cgaggacgat cagcacgacg acgctgaaga gggcgttgcg
    11641 gatgtcgtcg agcgccgaga ggatcgcgcc ggcggggtgg agccggcggg cggggagagc
    11701 gggggccgtg ggctggtcgg ccgcggacga cgctgccggc tgggcgggcg ccgccggctc
    11761 gtcgggcggg tcagacatcg tcgggacgct gcgcgagctc ggcgatccgg tcgcggatcg
    11821 cggcagcctc gtcgggacgc agcgccggga tcgtgtgcga gccgcccgcc gtgtggatgc
    11881 ggaccgagcg caggccgagc tggtccgaga gccaggtgcg ctgggtgtcg acgtgctgga
    11941 cgcggatcgt cgggatcagc gtgcgggtga tcacgaggac gccgcggcgg aggtcgatct
    12001 cctcgtcgcg cagctcgtag cgccagttgc gccagcggcg ctgcggctcg agcacgaggc
    12061 cgagcagggc gagcgcgagg gccgcgaggg tcagccccag cccgatcgag tcgggcgcgc
    12121 cgctctccgt gaggaccgag tcggcgatga aggagccgac gacgagcggg acggcccagc
    12181 cgagcgtccg gccggcgaac agcagcctcg cctgcccggg cagccgctgg gtcggctccg
    12241 gggttccgcc ctccatcgct agcggcagct cagagtcgcg cgtctacgac catcgcgccg
    12301 aacagcagcg cgaggtagag cagcgagtag aggtagaggc gcagcgccgc cggccggctc
    12361 gggttgcggt agagctggat cgcgaacgcg atgaagacgc cgccgagcgc gaccgacccg
    12421 acgaggtagg gcacgccgaa gccgccggcg cagaacggca gctgggtgat cgcgtagagc
    12481 agcagcgagt agaggacgat ctggcggcgg gtctcctgct cgccgcggac gaccggcatc
    12541 atcggcacgc cgaccttggc gtactcgtcc ttcatcagta gcgacagcgc ccagaagtgc
    12601 ggcggcgtcc agtagaagac gatcgcgaac aggtagagcg cggtccagct gaggctcccg
    12661 gtcgtggcgg cccagccgac gagcgggggc acggcgccgg ccgcgccgcc gatcacgatg
    12721 ttctgcggcg tggtgcgctt gagccagacg gtgtagacga gcgtgtagcc gaggaagccg
    12781 gacagcgaga gggcggccgc cagcgggttg accccgaccg cgagtaccac gcacgcgagc
    12841 gcggcgagcg cgatcccgta gaccagcgcg gcgttcggag cgacgcgacc cgaggcgacc
    12901 ggccggtcgg ccgtgcggcc catctgggcg tcgatgtcgc ggtcgtacca gtggttgacc
    12961 gcgccggccc cgcccgcgga gaggtagccg ccgaggcacg tcagggcgac gagtccgatc
    13021 gacggatcgc cggcgacgta catcgtcgtg accgtcgtga agatcagcag cgactggacc
    13081 ttcggcttgg tcatctcgac gtagtcggcg acgagctggc gcgtgcgcga gcgggcgcgc
    13141 ggcgcggcgg ctacggcgga ctggctgctg gctgcgggag gcggcatcgt ctaagcggtc
    13201 agcgcctcgc gattggacgc gccggagcgg gactctacgg gctcggggat gcggaagagc
    13261 tggaaggcga ggccgacgag cgtgccccag agcagcgttc cgagcgcgag gtgcaggacg
    13321 atcagcgcct cgtactcgtc gagccacacg ttgagcgcgc cgacgagcag ctgggtcgcc
    13381 agcaccgcgg cggctacctt tgcgctcagg accacgcccg ggctggggcg gcggcgcagc
    13441 gcgagaacga tcaacgtaac gacggcgatc gtcgtcaggt agacgaacac ccggtgggtc
    13501 aggtggatgt cgaccagccg cgtgacgccg aacggcagga agtcgccgtt gcaggtcggg
    13561 aactccttgc cgcaggcgtg gtgggcgccg tcgccgagct ggtagtcggc gcggccgtag
    13621 ttctgcgtgc ccgccatgta accgccggcg acgatcgtgc agaaggcgag accgctcgac
    13681 acgagcgcga gcggccggaa gccgcggccg ccgtcggccg gcggggcacc gagggtctcg
    13741 ggttgcgacg cgcgccagat gtagatcagc agcgccagca gcgccatcgc gagaccgagg
    13801 tgggcggcga cgagcgtctc gtccaggtcg ttctcgacgg tcgcggcgcc gagcaggccc
    13861 tgggcgatca ccagcagcgc tgcggcggcg gtcgcgcgca cgagcgcccg gttggcgcgg
    13921 tagcggcgcc aggcgagcac cgcgagcgcg atcatcatca gcccgacgat ccccgcgacc
    13981 gcgcggtggg tgtactcgat gatcgtgttg acgtcgaggc cggggatcag ggcgccgttg
    14041 cagaacggcc agccgtggaa gccggagtcg gcgggcccgc agccgaggcc ggaacccgac
    14101 acgcgcacga tcccgccgat caggatcagc acgaacgtcg tcgcgatcgt ggcgagggcc
    14161 agccggcggt aggtccgatc cgggcccgcg gcgccctcgg aaaggcccgc tgcgctgggg
    14221 gtcacgtgcc cgaggctacc ggctgcgcga cgctgccgac ggcctcgccg gagcgtgcga
    14281 cctcgcggcg gatgtccttc atcggctcga ccgagcggac gcgcggaacg acgtcgaagt
    14341 tgttgaccgg cggcggcgac tggacgaacc actcgagcgt gttgcccttc cacggatcgt
    14401 tgcccgcgac cttgccg
//
LOCUS       DATABV010000094         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_268912, whole genome shotgun sequence.
ACCESSION   DATABV010000094 DATABV010000000
VERSION     DATABV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268912"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VHF88_03915"
     CDS             complement(<1..1216)
                     /locus_tag="VHF88_03915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2160948.1"
                     /translation="MRTEVIDRRLALLGILALAVVVRLAGLGDRLSDAEGYSWLVASA
                     PSFDVFFDRLAAYENTPPLFYALLAPLPHDDEAWLRVPALLPALACVPVAYCLVRPLL
                     ETGVALLTALAVAVAPFHVSYSNYSRGFMLAALGLLLAAWAVARLAEGRRGRWWWLYL
                     AGGTLALQSEYDALLFLAPLLALPALWRRRGWREVVVLGALPLATLLPLTGEMLRGLD
                     QLDVTKLTPPSPDVSPASLRDSLVSLFTGTHGLAGSAAVRWAQLLGLVAALAVSATWL
                     WRRAGRLDRLDDAGRDVAFWLLVVAPATAVVLHAVVSVPGPEIFDSRYLVGLIPFACA
                     LLACGVAAIPGRWATPVAAACLLALAVAVVAQRHGRELHPDVAPAAELVSTSGVRTVL
                     TNSPVAAYYLTGG"
     gene            complement(1248..2780)
                     /locus_tag="VHF88_03920"
     CDS             complement(1248..2780)
                     /locus_tag="VHF88_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160949.1"
                     /translation="MRNSNTLRTLVCALLALAAVLLMLPAAANAGSRQLSMMQDDAEM
                     FGERAGEDPAAAMREMKELGVDVLRTNVLFYRIYDDFTDRRRPAGFDPSDPNSPQYTR
                     WGPTDNIVRLARQNGIKLLFTISGPGPFWASEQPRKCRGRGLCSWKPDAKVFGQFVEA
                     VAKRYRGQVDWYSLYNEPNIGRSSGGPGWLMPQATRTRAGRVETAGVMYRKLWQAGYK
                     AIAKYDRARRNRVLFGEVAAIGEPLPKLYAALCLDRDGKPFRGRLRRAHDCGRVSKLR
                     IGGWAIHPYNQGGFGPPNRTTNSKTALPQQYIPRLHRLMKRAAARGRVPGGRGIYITE
                     FGYQTNPPDRISTITPKLHARYINESDRLFFGDPRIKTVSQYQLVDVNDVSQYNSGLR
                     FAGGSKRKKASYDAYRLPIVVTRRSSSLVEVYGQVRPARQARLANVFSAPELQFAPRG
                     RGFSTIRRPRLNSVGIFRTNIRRGGAAAGRWRLKWRNGLIGGVSYSRVAKAGPALRYY
                     RD"
     gene            complement(2869..3093)
                     /gene="secG"
                     /locus_tag="VHF88_03925"
     CDS             complement(2869..3093)
                     /gene="secG"
                     /locus_tag="VHF88_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX2160950.1"
                     /translation="MEAILSGVQVILAVVVIVLVLMHSGKDAGLSGAFGVGTGAGPLG
                     GGSLVERNLTRWTIFFTILFTLNVLVLLKL"
     gene            complement(3122..3409)
                     /locus_tag="VHF88_03930"
     CDS             complement(3122..3409)
                     /locus_tag="VHF88_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160951.1"
                     /translation="MEYFIAVVVLLALVAAIVLRPLIWGVGDDDRESASREELEAAKE
                     AKYREIRDAQLDREMGKLSQEDHRAIDRELRAEAIEILKRLDDLEGRSPEA"
     gene            complement(3411..>4494)
                     /locus_tag="VHF88_03935"
     CDS             complement(3411..>4494)
                     /locus_tag="VHF88_03935"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX2160952.1"
                     /translation="LCVVIFWGTFFPLISEALSGEEASVGPPWFNRVTTPLALVLVAL
                     AAIGPMVPWRRTSRSGLVRSLRLPIAVTALTLVALLALTDSADSPLALVMFCFVATVL
                     TVVAQELTRGAAARRTITGDAWPRALGQLVSRNRRRYGGYLVHAGIAILFLGVAASSA
                     FQQQRDVRLSPGESFQAGGYDITYVRPTAEVGRDSAGTGAPITFGAVLRARRGDDVEI
                     LHPSRNYYPSQNAEAGAIGRFFEGEATSEVDVRWGLRRDLWAAVRPDIAELEPAIREA
                     DAKFSASRDDVQAIVIAAIVERYREDAPPATFRAIDSPLVSWIWIGGAIMILGALIAI
                     WPSPETRLRRVTSIYAARLGKELSRA"
BASE COUNT          700 a         1604 c         1627 g          563 t
ORIGIN      
        1 cgccgccggt caggtagtag gcggcgacgg gactgttggt cagcacggtc ctcacgcccg
       61 acgtgctcac cagctccgcc gccggcgcga cgtcggggtg cagctcccgg ccgtggcgct
      121 gggcgaccac ggcgaccgcg agcgcgagca gacaggccgc tgcgacgggc gtcgcccagc
      181 ggcccgggat cgcggcgacc ccgcaggcga gcagcgcgca agcgaatggg atgagcccga
      241 cgaggtagcg cgagtcgaag atctccgggc cgggcaccga gaccacggcg tgcagcacga
      301 ccgcggtcgc cggcgcgacg acgagcagcc agaacgccac atcgcggccg gcgtcgtcga
      361 gccggtcgag ccggccggca cgccgccaca gccaggtcgc cgagaccgcg agcgctgcga
      421 ccagcccaag cagctgagcc cagcgaaccg cagccgagcc cgcgaggccg tgcgtcccag
      481 tgaacagcga cacgaggctg tcgcgcaggc tcgccgggga gacgtcgggg gacgggggcg
      541 tcagcttcgt gacgtcgagc tggtcgagtc cgcgcagcat ctcgccggtc agcggcagca
      601 gcgtcgccag cgggagcgcg ccgaggacga cgacctcccg ccagccgcgc cgccgccaga
      661 gcgccgggag cgcgagcagc ggcgcgagga acagcagcgc gtcgtactcc gactgcagcg
      721 ccagcgtgcc gcccgccagg tacagccacc accaccggcc tcgccgcccc tcggcgaggc
      781 gcgcgacggc ccaggccgcc agcagcagcc cgagcgccgc cagcatgaag ccgcgcgagt
      841 agttcgagta cgagacgtga aacggcgcga ccgcgaccgc gagcgcggtc agcagcgcga
      901 cccccgtctc gagcagcggc cggacgaggc agtaggcgac cggcacgcag gcgagcgctg
      961 gcagcagcgc cggtacgcgc agccacgcct cgtcgtcgtg cggcagcggc gccaacagcg
     1021 cgtagaacag cggcggcgtg ttctcatagg cggcgaggcg gtcgaagaag acgtcgaacg
     1081 acggcgccga cgcgaccagc cacgagtagc cctcggcgtc gctcagccgg tcgcccaggc
     1141 ccgccagccg aaccacgacc gcgagcgcca ggatgccgag cagcgccagc cgccggtcga
     1201 tgacttcggt tctcacggcg ggggagcgta gcccccgccg ccgtcggcta gtcgcggtag
     1261 tagcgcagcg ccggtccggc cttcgcgacg cggctgtacg acacgccgcc gatcaggccg
     1321 ttgcgccact tgagccgcca gcgcccggcg gccgctccgc cgcggcggat gttcgtgcgg
     1381 aagatgccga ccgagttgag ccgtggccgc cggatcgtcg agaacccacg gccgcgcggg
     1441 gcgaactgca gctcgggcgc ggagaacacg ttcgccagcc gtgcctggcg cgccggccgc
     1501 acctggccgt agacctcaac caggctcgac gagcgccgcg tcacgacgat cgggagccgg
     1561 taggcgtcgt aggaggcctt cttgcgcttc gatccgccgg cgaagcgcag cccgctgttg
     1621 tactggctga cgtcgttgac gtcgacgagc tggtattgcg acacggtctt gatccgtggg
     1681 tcgccgaaga agagccggtc agactcgttg atgtagcgcg cgtgcagctt cggcgtgatc
     1741 gtcgagatcc ggtcgggcgg gttggtctgg tatccgaact ccgtgatgta gatgccacgc
     1801 ccgccgggca ctcggccacg cgcggcggcc cgcttcatca gccggtgcag gcgcgggatg
     1861 tactgctgtg gcagcgccgt cttgctgttc gtcgtgcggt tcggcggccc gaacccgccc
     1921 tggttgtagg ggtggatcgc ccagccgccg atccgcagct tggacacccg cccgcagtcg
     1981 tgcgcccggc gcagccggcc gcggaacggc ttgccgtcac ggtcgaggca gagcgccgcg
     2041 tagagcttcg gcagcggctc gccgatcgcc gcgacctcgc cgaacagcac gcggttgcgg
     2101 cgcgcccggt cgtacttggc gatcgccttg tagccggcct gccagagctt gcggtacatc
     2161 acgccggcgg tctcgacacg accggcccga gtgcgcgtgg cctgcggcat cagccagccg
     2221 gggccgccgc tggagcgacc gatgttcggc tcgttgtaga gcgagtacca gtcgacctgg
     2281 ccgcggtagc gcttggcgac cgcctccacg aactggccga agaccttcgc gtccggcttc
     2341 cacgagcaca gcccgcgacc acggcatttg cgcggctgct cagacgccca gaacgggccc
     2401 gggccgctga tcgtgaagag cagcttgatc ccgttctggc gggcgagtcg gacgatgttg
     2461 tcagtcgggc cccagcgcgt gtactgcggc gagttgggat cgctcggatc gaagccggcc
     2521 ggtcggcggc ggtcggtgaa gtcgtcgtag atccggtaga agaggacgtt cgtccgcagc
     2581 acgtcgacac cgagctcctt catctcccgc atcgccgccg ccgggtcctc gccggcacgc
     2641 tccccgaaca tctcggcgtc gtcctgcatc atcgagagct ggcgcgagcc ggcgttggcg
     2701 gcggccggca gcatcagcag cacggccgcg agggcgagca gcgcgcagac gagcgtgcgg
     2761 agggtgttcg agttcctcat gcgtcccttc ggtcgtgaag ccccggggag ggtggggcga
     2821 attcaacggt gtcgaagcgt acacacgccg cgacccggaa agttgcggct agagcttcag
     2881 cagcacgagc acgttgagcg tgaagaggat cgtgaagaag atcgtccagc gggtgaggtt
     2941 tcgctcgacg agcgagcccc cgccgagcgg gcccgcgccg gttccgacgc cgaatgcgcc
     3001 cgacagcccg gcgtccttgc ccgagtgcat cagcaccagg acgatgacca ccacggccag
     3061 gatgacctgt acgccgctga gaatcgcctc catcgccgac gcagactagc gaagcagcgc
     3121 gtcaggcctc gggggagcgg ccctcgaggt cgtccaggcg cttgaggatc tcgatcgcct
     3181 cggcgcgcag ctcgcggtcg atggcgcggt ggtcctcctg cgagagcttg cccatctcgc
     3241 ggtcgagttg agcatcgcgg atctcgcgat atttggcctc cttcgccgcc tcgagctcct
     3301 cgcggctggc cgactcgcga tcgtcgtcgc ctactcccca aatgagcggc cgcagcacga
     3361 tcgccgcgac gagcgccagc agcacgacca ccgcgatgaa gtactccacg ctaggcccgc
     3421 gagagctctt tgccgagcct ggcggcgtag atgctcgtca cgcgccgcag gcgcgtctcc
     3481 ggcgacggcc agatcgcgat cagcgcgccg aggatcatga tcgcgccgcc gatccagatc
     3541 cacgagacca gcggcgagtc gatcgcccgg aaggtcgccg gaggggcatc ctcgcggtag
     3601 cgctcgacga tcgcggcgat gacgatcgcc tggacgtcgt cgcgcgaggc tgagaacttg
     3661 gcgtcggcct cgcggatcgc cggctcgagc tcggcgatgt cgggccgcac ggccgcccag
     3721 aggtcgcgcc gcagccccca gcggacgtcg acctcgctgg tcgcctcgcc ctcgaagaag
     3781 cggccgatcg cgccggcctc ggcgttctgc gacgggtagt agttgcgcga cgggtgcagg
     3841 atctcgacgt cgtcgccgcg gcgcgcgcgc agcaccgcgc cgaaggtgat cggcgccccg
     3901 gtgccggcgc tgtcgcggcc gacctccgcc gtcggacgga cgtaggtgat gtcgtagccg
     3961 ccggcctgga aggactcgcc cggcgagagc cgcacgtcgc gctgctgctg gaacgccgac
     4021 gacgccgcca cgccgaggaa gaggatcgcg atcccagcgt ggacgaggta gccgccgtag
     4081 cggcgccggt tgcgcgagac cagctggccg agcgcgcgcg gccaggcgtc gccggtgatc
     4141 gtgcgccgcg ccgccgcgcc gcgcgtcagc tcctgggcga cgacggtcag caccgtggcg
     4201 acgaagcaga acatgacgag cgcgagcgga ctgtcggctg agtcggtcag tgccagcagc
     4261 gcgaccagcg tcagcgccgt cacggcgatc ggcagccgca gcgagcgcac cagcccggag
     4321 cgactcgtcc gccgccacgg gaccatcggc ccgatcgccg ccagcgcgac cagcacgagc
     4381 gccagcgggg tcgtgacgcg gttgaaccac ggcggcccga cgctcgcctc ctcgcccgac
     4441 agcgcctcgg agatcagcgg gaagaacgtc ccccagaaga tcaccacgca gagc
//
LOCUS       DATABV010000095        17957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2437882, whole genome shotgun sequence.
ACCESSION   DATABV010000095 DATABV010000000
VERSION     DATABV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437882"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VHF88_03940"
     CDS             complement(<1..908)
                     /locus_tag="VHF88_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2160953.1"
                     /translation="MGSSKAGVIATVAVALLAGGCGDSSTESGTAGGEPAGAGFPVTV
                     RDATRDVTIDRRPRRIVSLSPSATETLFAVGAGEQVVAVDDESDYPPDVPKTDISSYQ
                     PNVEAIAGYRPDLVVLPSIVPRDVTSGLRRLGLAVLAEPAPDDLDDAYHQIRELGLVS
                     GHAAAGRRLAREVRSKVERLIAAAPGGVAPKVFHELDPDLYSASSDTFIGRIYERLGL
                     RNVADAAARRAANPYPQLSSEAVVSANPDLVVLADSQCCAQTPAKVARRPGWRAVAAV
                     RNRAVVAIDEALASRWGPRIPLFVKRV"
     gene            complement(1126..2505)
                     /locus_tag="VHF88_03945"
     CDS             complement(1126..2505)
                     /locus_tag="VHF88_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX2160954.1"
                     /translation="MPSEPARPAILVAATHSGAGKTTVTGLVLRAVRRRGLSVQAFKI
                     GPDFIDSTYLAEATGRPAINLDLWMTGEHGVRGSYRRRSGDVDVSVIESMGALYDGAD
                     PSGHGSAAHIAKLLGVPVVVVLDVWGMTRTAGAILEGLLAFDRDVDIAACILNRVGSR
                     EHAAMIMDVLPASLRGLVVGSIERRSELEIPERHLGLLTVEENGASAAARDAAGAQAA
                     ELLDVDRLLAIAGARAPVEARKVPQAHRPTSRRPAARLAIARDDAFCFYYEENLELLR
                     EAGFELVPFAPTRDRKLPADTDAVYIGGGYPESFATELETNGALAGELRERATQGLPI
                     YAECGGLVYLARSLTGFDGARRAMSGVLPLDVAMDSDRLAIRYVEVRTRVASPLGGAG
                     TEARGQEFHQSHVVAADVAATLYDVTTSTGERYSDGYAWRSVVASYAHLHFGSNPELV
                     HNLLRAAVC"
     gene            complement(2609..2941)
                     /locus_tag="VHF88_03950"
     CDS             complement(2609..2941)
                     /locus_tag="VHF88_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160955.1"
                     /translation="MTGGEVPRGTGATGASRTDGVRHAARRLTTETKSFFKTSEWWAY
                     VVVLIGILIAGNSIEGEEGGADIFAADKVWLYVTLLTIGYMLSRGIAKSGVRDPYWAE
                     RGDSNADH"
     gene            complement(3082..3684)
                     /locus_tag="VHF88_03955"
     CDS             complement(3082..3684)
                     /locus_tag="VHF88_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160956.1"
                     /translation="MLAAAAAAVAALSAAPAANAGVLVASAPNCESQALERPFMRWLD
                     PLSYSLVPDGGFEQNAAGWTLSGARVVDGNEPFYVHGAGERKSLSLPRGSVATSPVMC
                     AGLDKPVMRFFAKSSGGLLSLSTLSVEVLFETASGQVRALPVGVVLPTTRWQPTLPLP
                     VLASLLPLLPGEQTPIAFRFRPLGGATWTIDDVYIDPLKR"
     gene            complement(3844..4836)
                     /gene="uvsE"
                     /locus_tag="VHF88_03960"
     CDS             complement(3844..4836)
                     /gene="uvsE"
                     /locus_tag="VHF88_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX2160957.1"
                     /translation="MESGAATAHRLGFAVKVLGDGGLPSHDTRRWGSSPHLRHSLERL
                     RAILDYLERHDIRMYRLPTALAPYASHPELTQFHGQVEECARELAAVGQIVRERDVRL
                     SSHPGQYTVLNSERPEVRDAAVAELEVQAALLDAMGLGPEAVVVLHVGGATGGVNAAL
                     DRFAAALERLSERARARLVVENDDRSFGLVDVLALSERTGLRVVWDVLHHRCHDPARM
                     SNRDALAAALATWPEDTVPKIHYSSPRLDVEQRRVRHRRRVEQRLVLPQLRAHADLID
                     PIGFEQFLRDASAGRDFDVMLEAKAKDVALLRLRDQLAERGLASHRGRLRVAPT"
     gene            complement(4867..5760)
                     /locus_tag="VHF88_03965"
     CDS             complement(4867..5760)
                     /locus_tag="VHF88_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX2160958.1"
                     /translation="MDSPLLSEILLPVAIATIMGSLGLALTPADFRRVLVAPRGVAIG
                     MLNLALISPLLAFAMAELFDLPPELAVGLVLLGASPGGMMANMLTHLARGETALSVTM
                     TAISSIASVVTVPLFLGLATEHFGAGDLGDVSMPGVVALVFAITIVPVAIGMTLRQRW
                     PDRVLEIYPRVRAVSLSLFGIVVLGAIASEYDTVAENVEAVAGAAFALNIAAMTISYL
                     MSRVARLGSRQSTAIALELGVHNSTLAIAVGATLAADLAIPAAVYSSFMLFTGGAFAW
                     AMSRRNAEALSLEADPVVPAG"
     gene            complement(5802..7484)
                     /locus_tag="VHF88_03970"
     CDS             complement(5802..7484)
                     /locus_tag="VHF88_03970"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEX2160959.1"
                     /translation="MTLDADVIVVGAGLAGLVATAELADAGRKVLLLDQENEANLGGQ
                     AFWSLGGLMFVDSPEQRRCRVKDSYELALQDWLGTAAFDRPEDEWPRKWAEAYVGFCA
                     GEKRPWLHAQGMRWIPNPGWPERGGYHADGHGNSVPRFHITWGTGPGVVAPFERRVRE
                     AVAKGLVQLRFRHRVDELSTTDGTVDGVRGTLLAPSATARGVASNRDPAGEFELHAQA
                     VIVTGGGIGGNHDLVREKWPRDRLGEPPARMISGVPAHVDGRMLAIARAAGANEINAD
                     RMWHYVEGIHNWDPIWPKHAIRIIPGPSSLWFDARGQRLPGPLYPGYDTLGTLDHIQR
                     TGHDYTWFVLTQKIIEKEFALSGSEQNPDTTSKSMRAVLRERLGHGPPTPVQAFMDNG
                     EDFIVERDLATLVRRMNELSGDDLIDLADLEGQIAARDREIANPYAKDGQVTAIHGAR
                     AYFADKVTRVAAPHRVLDPKAGPLIAVRLSILTRKSLGGLQTDLDSRALRPDTASGGT
                     GGEPVPGLYAAGEVAGFGGGGVHGYRSLEGTFLGGCLFSGRIAGRAAAAAVA"
     gene            7539..8429
                     /locus_tag="VHF88_03975"
     CDS             7539..8429
                     /locus_tag="VHF88_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160960.1"
                     /translation="MQHRIRRSLSYANVMSTIAVFTVLGGGAYAAVEKIGAKDIKRNA
                     VRAKHIKKSQVAPRHLKRNAVTAAKVASGAVTSPKLASGAVTDGKLADGAVTNGKLAN
                     GSVSDVKLIDGAVVGPKLADGAVTNGKLADGSVTGAKVDESTLGPVPDASRLDGLDSV
                     DFVHGAGRLHGGVLVDPLGGPPTAIQLDVGTLALECRNPASVGSDFIFTNTSGASVHI
                     WTERMQGTPLPVAPSVAYGSIANGGTAAVGISGPVVQSGQSMIGFTVASGTRSTKIEA
                     RLVYTGEGCVAPLTIAELRG"
     gene            complement(8450..8665)
                     /locus_tag="VHF88_03980"
     CDS             complement(8450..8665)
                     /locus_tag="VHF88_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160961.1"
                     /translation="MRWSRLAQTAKKTIDSRGGTEGLKRDAEQLREIARGQGSAKDKA
                     RRAAEALKQPTGHRGPGEPPRRDAGGL"
     gene            8787..10358
                     /locus_tag="VHF88_03985"
     CDS             8787..10358
                     /locus_tag="VHF88_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2160962.1"
                     /translation="MAARIVTPMLDRFVNHSWLVGDPTTPGRKLGARVRWLTATAIVF
                     ANGVGAAVVICFALFVLPKPDGVDGPEVTWSNLGLALAYLTVAMAVGVRWGRRLVEGG
                     RGGIRGWLDEDRQPTPEEQLRVLRAPLRIMLVEAVLWGVAVVCFAALNAVFSPLLAVG
                     VGSTVALGGLTTSSVAYLLTELALRPVASRALATGAPDRRGVPGVTARWLLAWALGTG
                     APIVGLALVGIVALTDVEIDEGPLAVTAVTLSGIALAFGAIASLLAAYATVHPIGSIR
                     RGLAKVQRGDFDVRLGVWDSTEIGLLQAGFNEMVAGLRERERIRDLFGRQVGREVAQQ
                     ALAGGEVDLGGVECEVAVLFVDVVGSTELAATRPPREVVELLNRFFGEVVDAVEHNGG
                     WINKFEGDAALAIFGAPGRLEDAEGRALRAAREIDERLRARVGELAAGIGVASGTAVA
                     GHIGAEQRFEYTVIGDPVNEAARLSELAKARPARVLASSVTIDRAGDESARWQLGEPI
                     ELRGRGVPTVVASPL"
     gene            complement(10355..11029)
                     /locus_tag="VHF88_03990"
     CDS             complement(10355..11029)
                     /locus_tag="VHF88_03990"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEX2160963.1"
                     /translation="MRKFLHDNSLSVFFLAIFLIALALQSLAGWQDFNNSQLRHDGES
                     IGYGRFLLSSEFGVAMLENWQSEYLQFALYITATVWFLQRGSPESKELDQAGGESDEE
                     QKVGRHADEDSPLWARVGGARTRVFSNSLLIVMGVIWLLSWIGQSITGRVAYNEERLE
                     HHQSGLDYWQYIGSADFWERTFQNWQSEFLAVGSMAILAVYLRQRGSPESKPVGEGHE
                     HTGVTG"
     gene            complement(11109..11513)
                     /locus_tag="VHF88_03995"
     CDS             complement(11109..11513)
                     /locus_tag="VHF88_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160964.1"
                     /translation="MGATPQRALLILAVGLALTGCGATDSDGAADAAERLYSAYSEDD
                     GAAACAVLSDDTREQLVKDEQKPCPEAVLALKLSGQRVTAEEAYITEAKVETDGGDSV
                     FVEETGDGWLVTAAGCRPAPGRESPYDCEVES"
     gene            complement(11576..11986)
                     /locus_tag="VHF88_04000"
     CDS             complement(11576..11986)
                     /locus_tag="VHF88_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2160965.1"
                     /translation="MPEVRSGAGLVDTSVLVSPADSAFLPTEIAVSALTAAELAAGPH
                     ATDSPAERGRRQERLQIVEAMHEPLPFDAECARAYGRIYAAIVATGRKARGRRAVDLL
                     IAATAVAHQLPLYTRNAGDLAGLEPVLEIVDCSP"
     gene            complement(11979..12233)
                     /locus_tag="VHF88_04005"
     CDS             complement(11979..12233)
                     /locus_tag="VHF88_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160966.1"
                     /translation="MPRELTQRELRNESGAIMRALDRGERFVVTRNGVAVGELSPIGR
                     RRFVGAEALLDAFARTPPVDAARLRADLDALADQAADPRA"
     gene            12313..12531
                     /locus_tag="VHF88_04010"
     CDS             12313..12531
                     /locus_tag="VHF88_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2160967.1"
                     /translation="MSPLAHRAFGANLAFERKRRALSMNALARLAGTHASEISRLERG
                     QRDPRLSTMVRVSRALEVPLADLLRGVR"
     gene            12607..12966
                     /locus_tag="VHF88_04015"
     CDS             12607..12966
                     /locus_tag="VHF88_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160968.1"
                     /translation="MAELTPIDEKLAEVLGLAQASQDTVSKVLKLCDDEQVVETLERM
                     RDEARETQERCEAIAAELDGKKTAVEEKARETKQEASQMRDDYLGDDADDLDGLEFMI
                     MAEAGELGHVEIVGAMA"
     gene            complement(13141..13980)
                     /locus_tag="VHF88_04020"
     CDS             complement(13141..13980)
                     /locus_tag="VHF88_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160969.1"
                     /translation="MKRSHAVIALAIVAALVVALPATGAAPNPLKVAKKALKIGKKAD
                     KRARKAARTANRALKTGRAAGTQAGVATRTADQALETGRAADTRARQALGVLAGSVPN
                     ATRAQSAGVADSLQGMRIARFNTRVAPSATATPQTAARAAAQRVPLLSEGPITVYGKC
                     YAVNTDPANPSVEGEIYVETSAAGAVFAGDEGDSGSGLLGPATPEEDRDLLRTSSQAG
                     VGNPGTLNTSGASAGPFYVIAPGVAIHGQLAIATKVGSPPAGDGVLGAGNACIFTGIA
                     NAS"
     gene            14123..14395
                     /locus_tag="VHF88_04025"
     CDS             14123..14395
                     /locus_tag="VHF88_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX2160970.1"
                     /translation="MGILGWIVLGLLAGAIAKAILPGDDPGGIFVTMLIGIAGAIIGG
                     LIASALDIGDLDEFFDIGTWLIAIAGSLLLLLVYRMVVGRRDDRVT"
     gene            complement(14432..15349)
                     /locus_tag="VHF88_04030"
     CDS             complement(14432..15349)
                     /locus_tag="VHF88_04030"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX2160971.1"
                     /translation="MATAEDDEIDALRRRGFAIAYRMLGSVGDAEDVVQEALLRLHRA
                     RAAGERIESPQAYVSTVVTRLAIDQLRSARARREQYVGEWLPEPLVTATDDLPPERAE
                     LADSLSFAFLVLLESLTPEQRAALLLRDVFDYPYDEVAAIVGKSEDNARQLATRARKA
                     VEARRPRFDASRERRDALADRFFAAAAEGDLAGLEALLADDVALHGDGGGKVPALARA
                     LSGANRVARTLVNWAKAGRRFGGPFVRRVDLNGQPGALLLDADGRLFGAMTLEIADGR
                     VQAIHSVVNPDKLAHLGQVADMQALLRGE"
     gene            complement(15357..16307)
                     /locus_tag="VHF88_04035"
     CDS             complement(15357..16307)
                     /locus_tag="VHF88_04035"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2160972.1"
                     /translation="MKILVAGATGALGRQLVPRLVERGHEVTGMTRRDAKRDLLRSLG
                     ATPVVADALDPDAVARAVAEAEPEVVVHQLTALAGGLDARHIERTFAMTNRLRTEGTD
                     HLLAAARAVGARRFVAQSFAGWPYARDGAPVKSEDEPLDPDPPRQLRTTHAAIRHLEQ
                     AVTGAGGIEGVVLRYGGFYGPGTSMALEPEGEQVAEIRARKFPVVGDGGGVWSFVHVA
                     DAAEATALAIERGAPGIYNVVDDSPAPVREWLPATAEAIGAKRPLRVPRFVGRLAAGE
                     AATVMMTEVRGASNAKAKRELGWQPRHRSMWESLAGEARG"
     gene            complement(16394..17479)
                     /locus_tag="VHF88_04040"
     CDS             complement(16394..17479)
                     /locus_tag="VHF88_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX2160973.1"
                     /translation="MALTVAATIVLAVACGGDDDGGGAGLTTQSAGSPTLPLRQAVGQ
                     LLVMAFDGTSAPGYVRRRLRDGEGAGVILFGGNVVDATQLRRLTRSIQGAARSGALIA
                     TDQEGGEIRNVPFAGPAAPQSATATPAEARAAATEAARGLRSAGVNVNLAPVADVEPP
                     IGGGALPGRLYPGDPRQVAALTTAAIQAHERAGVAATVKHYPGLGRATVNTDDGSATI
                     DAPRAELEAADLEPYRAAVAADAPLVMAGHALYPAFDPDRIASQSRTLLTDVLRDQLG
                     FRGVAITDSIEAQAVLDRSDVATAAVRSIAAGADIVLMTGSGSWNLVYPRLLRAARRS
                     PAFRSRVRESAERVLALKRRLGLRAGP"
BASE COUNT         2481 a         6342 c         6610 g         2524 t
ORIGIN      
        1 acgcgcttga cgaacagcgg gattcgcggg ccccagcgcg acgcgagcgc ctcgtcgatc
       61 gccacgaccg cccggttccg gacggcggcc accgcacgcc agcccggccg gcgagcgacc
      121 ttggcgggcg tctgcgcgca gcactgcgag tccgccagca cgacgaggtc ggggttcgcc
      181 gacacgaccg cctccgagga gagctgcggg tacgggttgg ccgcgcgccg cgctgccgcg
      241 tcggcgacgt tgcgcaggcc gagccgctcg tagatgcgcc cgatgaacgt gtccgagctg
      301 gcgctgtaga ggtcggggtc gagctcgtgg aagaccttcg gcgcgactcc gccgggcgcg
      361 gcggcgatca ggcgctcgac cttcgagcgg acctcgcgcg cgagccggcg gccagccgcc
      421 gcgtgaccgc tgaccagacc gagctcgcgg atctggtggt acgcgtcgtc gaggtcgtcc
      481 ggcgcgggct cggcgagcac cgccaggccg agccggcgca ggccggacgt cacgtcgcgc
      541 ggcactatcg acggcagcac gacgaggtct ggccggtagc cggcgatcgc ctcgacgttg
      601 ggctggtagg aggagatgtc ggtcttcgga acgtcgggcg ggtagtcgga ctcgtcgtcg
      661 accgcgacca cctgctcgcc ggcgccgacg gcgaacagcg tctcggtcgc cgacggcgac
      721 agcgacacga tccggcgcgg ccgccgatcg atcgtgacgt cgcgcgtcgc gtccctcacg
      781 gtgaccggaa agcccgcgcc cgccggctcg cctcccgcgg tccccgactc ggtggacgag
      841 tcgccgcagc cgccagcgag cagcgccacg gcaaccgtgg cgatcacccc cgccttgctc
      901 gatcccatca aaaacctccg cgtgtcaggc gaaggaagtc agctcggacg gaggaccccg
      961 tgcgaaccgc ccttccttcg agggccgatc cgtcgacacg ggtcaggcga cctggctcat
     1021 cccctccatg ggggcatcgc agttgcggga cagcgccggg ttctcaccgg acttcgctgg
     1081 cttcgtgcca gccggggggt gtatgagccc cagccgcggg cgaccctaac agaccgcggc
     1141 gcgcagcagg ttgtggacga gctcggggtt cgacccgaag tgcaggtgcg cgtagctggc
     1201 gaccaccgac cgccaggcgt agccgtcgct gtagcgctcg ccggtgctcg tggtgacgtc
     1261 gtacaacgtc gccgcgacat cggccgcgac gacgtgcgac tgatggaact cctgcccacg
     1321 cgcctcggtg ccggccccgc ccagcggcga cgcgacgcgc gtgcgcacct cgacgtagcg
     1381 aatcgcgagc cggtcggaat ccatggcgac gtcgagcggc agcacgccgc tcatcgcacg
     1441 cctggcgccg tcgaagccgg tcagcgagcg cgccaggtag acgaggccgc cgcactcggc
     1501 gtagatcggc agcccctgcg tggcgcgctc gcgcagctcg cccgcgagcg ccccgttggt
     1561 ctcgagctcg gtcgcgaagc tctccggata gccgccgccg atgtaaaccg cgtcggtgtc
     1621 ggccggcagc ttccggtccc tcgtcggagc gaacggcacg agctcgaagc cggcctcgcg
     1681 cagcagctcg aggttctcct cgtagtagaa gcagaacgcg tcgtcacggg cgatcgcgag
     1741 gcgcgcggcc ggccggcgcg aagtcggtct gtgggcctgc gggaccttgc gcgcctcgac
     1801 gggcgcgcgg gcgcccgcga tggcgagcag ccggtcgacg tcgagcagtt ccgccgcctg
     1861 cgcgccggcg gcgtcgcgcg ccgcggccga cgccccgttc tcctctacgg tgagcagtcc
     1921 gaggtggcgc tcggggattt cgagctctga ccgccgctcg atcgagccga cgacgaggcc
     1981 ccgcagggat gcggggagga cgtccatgat catcgctgcg tgctcccggc tgccgacgcg
     2041 gttgaggatg caggccgcga tgtcgacgtc ccgatcgaac gcgagcagcc cttcgaggat
     2101 cgcacccgcg gtgcgggtca tgccccagac gtcgagcacg acgaccaccg ggacgccgag
     2161 cagcttggcg atgtgcgccg cgctgccgtg gccagagggg tccgctccgt cgtagagggc
     2221 ccccatcgac tcgatcaccg acacgtcgac gtcgccggac cggcggcggt agctgccgcg
     2281 aacgccgtgc tccccggtca tccagaggtc gaggttgatc gccggccgcc cagtcgcctc
     2341 ggcgagatag gtggagtcga tgaagtcggg cccgatcttg aaggcctgca ccgagagccc
     2401 gcgacggcgc acggctcgca ggacgaggcc ggtgaccgtc gtctttccgg cgccgctgtg
     2461 cgtcgcggct acgaggatcg ccggccgggc cggctcgctg ggcatggcag ccaggaagta
     2521 tggcccgcgg cgggacgagc ccggcgaagc cgccttcggg atggcatccc gcccggccgg
     2581 tcagaccggg cgggaccccc tcgagccgct agtggtcggc gttgctgtcg ccgcgctcgg
     2641 cccaatacgg gtcgcgcacg ccggacttcg cgatgccgcg gctcagcatg tagccgatcg
     2701 tcagcagggt gacgtagagc cagaccttgt cggcggcgaa gatgtcggcg ccgccctcct
     2761 cgccctcgat cgagttgccg gcgatcagga tcccgatcag gaccacgacg tacgcccacc
     2821 actcgctcgt cttgaagaac gacttggtct cagtcgtcag acggcgcgcc gcatgacgga
     2881 ctccgtccgt gcggctcgcg cccgtagcgc cagttcctct gggcacctct ccaccggtca
     2941 tgtcactcct tctgtcgttt ggccaatccg gacacgggaa gctcccgtgc gccggttggt
     3001 gccaacggat gcccccgtcc ccgccagctc aaacgcataa aagcgtgccg ctatcgcaag
     3061 cgatagcggt acacctgctc ctcagcgctt cagtggatcg atgtagacgt cgtcgatcgt
     3121 ccacgtcgcc ccgccgagcg ggcggaagcg gaaggcgatt ggggtctgct cgcccggcag
     3181 cagcggcaga aggctcgcca gcaccggcag cggcagcgtc ggctgccagc gcgtggtcgg
     3241 cagcaccacg ccgaccggca acgcgcgcac ctggccgctc gccgtctcga acagaacctc
     3301 gaccgacagc gtcgagagcg acagcaggcc accggagctc ttggcgaaga agcgcatgac
     3361 gggcttgtcg agccccgcgc acatcacagg gctggtcgcg acgctgccgc gcggcagcga
     3421 gagcgacttg cgctcgccgg ctccgtggac gtagaagggc tcgttgccgt cgacgacccg
     3481 cgcccccgag agggtccagc cggccgcgtt ctgctcgaag ccgccgtccg ggacgaggct
     3541 gtagctgagc ggatcgagcc agcgcatgaa cgggcgctcg agcgcttgcg actcgcagtt
     3601 cggcgccgag gcgaccagca cgccggcgtt cgccgcgggc gcggcggaca gcgccgccac
     3661 ggctgcggcc gcggccgcca acatcgttac tcgaatcctt ccgctcactt gaaactcccc
     3721 tgtcgttgat tcagcggaat cagagtgagg gcacttctcg gttctcgcca cagagatatc
     3781 cgtctaatac gtacggccag taggcataga aggcggattt cccctttggg agcggaagaa
     3841 aggtcaggtg ggcgctacgc gcagccggcc gcggtggctt gcaagacccc gctcggcgag
     3901 ctggtcgcgc agccgcagca gtgccacgtc cttcgccttc gcctcgagca tcacgtcgaa
     3961 gtcgcgcccc gcggacgcgt cgcgcaggaa ctgctcgaag ccgatcgggt cgatcaggtc
     4021 ggcgtgggcg cgcagctgcg gcaagacgag ccgctgctcg acacgacggc ggtggcgcac
     4081 gcgacgctgc tcgacgtcga ggcgcgggga ggagtagtgg atcttcggca ccgtgtcctc
     4141 cggccaggtg gcgagtgcgg ccgccagcgc gtcgcggttc gacattcgcg ccgggtcgtg
     4201 gcagcggtgg tgcaggacgt cccagacgac acgcagcccg gtgcgctcgg agagcgccag
     4261 cacgtcgacg agcccgaacg agcggtcgtc gttctcgacg accaggcggg cccgggcgcg
     4321 ctcggagagc cgttcgagcg cggcggcgaa gcggtcgagc gccgcgttga cgccacccgt
     4381 tgccccgccg acgtgcagca cgacgaccgc ctcgggcccg agacccatcg cgtcaagaag
     4441 cgccgcctgc acctcgagct cggcgaccgc ggcgtcgcgc acctcgggcc gctcggagtt
     4501 cagcaccgtg tactggccgg ggtgcgagga cagcctgacg tcgcgctcac gcacgatctg
     4561 tccgaccgcg gcgagctccc gggcgcactc ctcgacctgt ccgtggaact gggtcagctc
     4621 cggatggctc gcgtacggcg cgagcgcggt cggcagccgg tacatgcgga tgtcgtggcg
     4681 ctcgaggtag tcgaggatcg cgcgcaaccg ctcgagcgag tggcgcaggt gcgggctgga
     4741 gccccagcgg cgcgtgtcgt gcgacggcag gccgccgtcg ccgagcacct tgacggcgaa
     4801 gccgagccgg tgggccgtgg cggcgccgct ctccacctcc tcaggatggc agcggccggc
     4861 cgtctgctag cccgccggca ccaccgggtc cgcctccaac gacagcgcct ccgcgttgcg
     4921 cctgctcatc gcccacgcga aggcgccgcc ggtgaagagc atgaacgagc tgtagaccgc
     4981 tgccgggatg gccaggtccg cggcaagcgt ggccccgacg gcgatcgcca gcgtcgagtt
     5041 gtgcacaccg agctcgagcg cgatcgcggt cgattggcgg ctgccgaggc gggcgaccct
     5101 cgacatcagg tagctgatcg tcatcgcggc gatgttgagc gcgaacgcgg cgccggcgac
     5161 cgcctcgacg ttctcggcga ccgtgtcgta ctcgctcgcg atcgctccca gcacgacgat
     5221 tccgaacaga ctcagcgaca ccgcgcgcac gcgcgggtag atctccagga cccggtccgg
     5281 ccaacgctgg cgcagcgtca tcccgatcgc gaccgggacg atcgtgatcg cgaagaccag
     5341 cgcgaccacg ccgggcatcg acacgtcgcc gaggtccccg gcgccgaagt gctcggtcgc
     5401 caggccgagg aagagcggca ccgtcaccac cgacgcgatg ctgctgatcg ccgtcatcgt
     5461 caccgagagc gcggtctccc cgcgcgccag gtgggtcagc atgttggcca tcatcccgcc
     5521 gggcgaggcg ccgagcagga ccagcccgac cgccagctcg ggcggcaggt cgaacagctc
     5581 ggccatcgcg aacgcgagca gcggcgagat cagcgccagg ttcagcatgc cgatcgcgac
     5641 gccgcgcggg gcgaccagca cgcgccggaa gtcggccggc gtcagcgcca gcccgaggct
     5701 gcccatgatc gtcgcgatcg cgactggcag caggatctcg gatagcagcg gactgtccac
     5761 ggcagggcga gcctagcctg cgccggttgg cgcggtagcg atcacgccac cgcggccgcc
     5821 gccgcgcggc cggcgatccg ccccgagaac aggcagccgc cgaggaacgt gccctcgagc
     5881 gagcggtagc cgtgcacgcc cccgccgccg aagccggcga cctcgccggc ggcgtagagg
     5941 ccggggaccg gctcgccgcc tgtgcccccg gaggcggtat cgggccgcag cgcgcgcgag
     6001 tcgaggtcgg tctgcaggcc gccgagcgac ttgcgcgtga ggatcgacag gcgcaccgcg
     6061 atcagcgggc cggccttggg gtcgaggacg cggtgcgggg cggcgacgcg cgtcaccttg
     6121 tcggcgaagt aggcgcgcgc gccgtggatc gcggtcacct gcccgtcctt ggcgtagggg
     6181 tttgcgatct cgcggtcgcg tgcggcgatc tggccctcga gatcggcgag gtcgatcagg
     6241 tcgtcgccag acagctcatt catccgccgc acaagggtcg cgaggtcgcg ctcgacgatg
     6301 aagtcctcgc cgttgtccat gaacgcctgc acgggcgtcg gcggcccgtg gccgagccgc
     6361 tcccggagca ccgcgcgcat gctcttcgac gtcgtgtcgg ggttctgctc ggagcccgac
     6421 agcgcgaact ccttctcgat gatcttctgg gtcaggacga accacgtgta gtcgtggccg
     6481 gtgcgctgga tgtggtcgag cgtgccgagc gtgtcgtagc cggggtagag cgggccgggc
     6541 agccgctgcc cgcgcgcgtc gaaccacagc gacgacgggc cggggatgat ccggatcgcg
     6601 tgcttcggcc aaatcgggtc ccagttgtgg atgccctcga cgtagtgcca catccgatcg
     6661 gcgttgatct cgttggctcc ggcggcgcgc gcgatcgcga gcatacggcc gtcgacgtgg
     6721 gcggggacgc cggagatcat ccgcgcgggc ggctcgccga gacggtctct gggccacttc
     6781 tcacgcacga ggtcgtggtt gccgccaatg ccgccgccgg tgacgatcac cgcctgtgcg
     6841 tgcagctcga actcgccggc ggggtcacgg ttcgaagcga cgccgcgcgc cgtcgcgctc
     6901 ggcgcgagca gcgtgccgcg tacgccgtcg actgtgccgt cggtggtcga cagctcgtcg
     6961 acgcggtggc ggaagcgcag ctgcaccaac cccttggcga ccgcctcacg cacccgccgc
     7021 tcgaacggcg cgacgacgcc cggcccggtg ccccaggtga tgtggaagcg cggcaccgag
     7081 ttgccgtgcc cgtcggcgtg gtagccgccg cgctccggcc agcccggatt ggggatccag
     7141 cgcatcccct gcgcgtgcag ccacgggcgc ttctcgccgg cgcagaagcc gacgtaggcc
     7201 tcggcccact tgcgcggcca ctcgtcctcc ggccggtcga acgccgccgt cccgagccag
     7261 tcctgcagcg ccagctcgta ggagtccttg acgcgacagc gccgctgctc ggggctgtcg
     7321 acgaacatca gcccgccgag cgaccagaac gcctgcccgc cgaggttcgc ctcgttctcc
     7381 tggtcgagca gcaggacctt gcgccccgcg tcggcgagct ccgccgtcgc caccagcccg
     7441 gcgagcccgg cgcccaccac gatcacgtcc gcgtcgagag tcatgttgcg aaccgtacgg
     7501 tcttcgggca cgcccgtgtc tccagaggag gtctcgcgat gcagcaccgg atcaggcgca
     7561 gcctgagcta cgccaatgtc atgtcgacga tcgccgtgtt cacggtgctc ggcggcggcg
     7621 cctacgcggc agtcgagaag atcggcgcca aggacatcaa gcgcaacgcc gtgcgcgcga
     7681 agcacatcaa gaagagccag gtagcccccc ggcacctgaa gcgcaacgcg gtcacggcgg
     7741 cgaaggtcgc cagcggcgcg gtgacgagcc cgaagctcgc gagcggcgcc gtcaccgacg
     7801 gcaagctggc ggacggtgcc gtcaccaacg gcaagctcgc gaacggctcg gtcagcgacg
     7861 tcaagctgat cgacggcgcc gtcgttgggc caaagcttgc cgacggtgcg gtgacgaacg
     7921 gcaagctcgc cgacggatcg gtcacgggcg ccaaggtcga cgagtcgacg ctcgggccgg
     7981 tgccggatgc gagccgactc gacggcctcg actcggtcga cttcgtccac ggcgccgggc
     8041 ggctgcacgg cggcgtcctg gtcgatcccc tgggcggtcc gccgaccgca atccagctcg
     8101 acgtcggcac cctagcgctc gagtgccgca atccggcgtc ggtcggctcg gacttcatct
     8161 tcaccaacac gagcggagcc tccgtccaca tctggacgga gcgcatgcag ggcacgccgc
     8221 tgccggtagc cccgtccgtc gcctacggct ctattgcgaa tggcggtacg gccgctgtcg
     8281 gaatatccgg gccggtcgtg cagagcggcc agagcatgat cggcttcacg gtcgcgagcg
     8341 gcacacgatc gacgaagatc gaggcgaggc tcgtctacac cggggagggc tgcgtcgcgc
     8401 cgctgacgat cgcggagctg cgcggctaag cccgccggtg ggccggcggc tagaggccgc
     8461 cggcgtcgcg gcgcggcggc tcgcccggac cgcgatggcc ggtcggctgc ttcagcgcct
     8521 cggcagcccg gcgcgccttg tccttcgccg agccctgccc gcgcgcgatc tcgcgcagct
     8581 gctctgcgtc gcgcttcagt ccctcggtcc cgccacggct gtcgatcgtc ttcttcgccg
     8641 tctgggccag tcgactccac ctcatcgtcg ctcctttcgc acgcgtaacc ggcacggtcg
     8701 caacgtcacc gctacccggt cgtccaccgt cgcacacacg gggccgcggc gcccccgcgg
     8761 cgcgacaccg gtagtacttg ccgccgatgg ccgcgcggat cgtcacgccg atgctcgacc
     8821 gcttcgtcaa ccactcgtgg ctggtcgggg atccgacgac gcccgggcgc aagctcgggg
     8881 cgcgcgtgcg ctggctcacc gcgacggcaa tcgtgttcgc caacggcgtc ggcgccgccg
     8941 tcgtcatctg cttcgcgctg ttcgtcctgc cgaagcccga cggcgtcgac gggccggagg
     9001 tcacctggtc gaacctaggc cttgcgctcg cgtacctgac ggtcgcgatg gcggtcggcg
     9061 tgcgctgggg acgacggctg gtggagggcg ggcgcggcgg catccgtggt tggctcgacg
     9121 aggatcgcca gccgactccc gaggagcagc tgcgcgtcct gcgtgccccg ctgcggatca
     9181 tgctcgtcga ggccgtgctc tggggggtgg cggtggtctg cttcgcggcg ctgaacgcgg
     9241 tcttctcgcc gctgctggcg gtcggagtcg gatcgaccgt cgcgctcggc gggctgacca
     9301 cctcgtcggt cgcctacctg ctgaccgagc tggcgctgcg gccggtcgcc tcacgggcgc
     9361 tcgccactgg cgcccccgac cggcgcgggg tgcccggcgt gacggcgcgc tggctgctgg
     9421 cgtgggcgct cggcaccggc gcgccgatcg tcggcctcgc gctcgtgggg atcgtcgcgc
     9481 tgaccgacgt cgagatcgac gagggcccgc tcgcggtcac cgcggtcacg ctgagcggga
     9541 tcgcgctcgc cttcggagcg atcgcgtcgc tgctcgccgc ctacgcgacc gtccacccga
     9601 tcggctcgat ccggcgcggc ctggccaagg tccagcgcgg cgacttcgac gtgcggctcg
     9661 gtgtctggga cagcaccgag atcggcctgc tgcaggccgg tttcaacgag atggtcgcgg
     9721 ggctgcggga gcgtgagcgg atccgcgacc tgttcgggcg acaggtcggc cgcgaggtcg
     9781 cgcagcaggc gctggcgggc ggcgaggtcg acctgggtgg ggtcgagtgc gaggtagcgg
     9841 tgctgttcgt cgacgtcgtc ggctcgacgg agctggcagc cacgcgcccg ccgcgcgagg
     9901 tggtcgagct gctcaaccgc ttcttcggcg aggtggtcga cgccgtcgag cacaacggcg
     9961 gctggatcaa caagttcgag ggcgacgcgg cgctggcgat cttcggggcg ccggggcgac
    10021 tcgaggacgc ggaggggcgg gcgctgcgtg ccgcgcgaga gatcgacgag cgcctgcggg
    10081 cgcgggtcgg ggagctggct gccgggatag gggtcgcgtc cggcacggcc gtcgccggcc
    10141 acatcggcgc cgagcagcgg ttcgagtaca cggtgatcgg cgacccggta aacgaggcgg
    10201 cgcggctcag cgagctcgcg aaggcgcgtc ccgcgcgcgt gcttgcctcg agcgtgacga
    10261 tcgaccgcgc cggtgacgag tcggcgcgct ggcagctcgg cgagccgatc gagctccgcg
    10321 gccgcggcgt cccgacggtc gtcgccagcc cgctctagcc ggtcacgccg gtatgctcgt
    10381 gtccctcgcc gaccggtttg gactcgggcg agccgcgctg gcggaggtac accgcgagga
    10441 tcgccatcga ccccaccgcg aggaactcgg actgccagtt ctggaatgtc cgctcccaga
    10501 agtcggcgct gccgatgtac tgccagtagt ccagcccgct ctggtggtgc tcgagccgct
    10561 cctcgttgta ggcgacgcgc ccggtgatcg actgcccgat ccaggagagc agccagatca
    10621 ctcccatgac gatcagcagc gagttcgaga agacgcgcgt ccgcgcgccg ccgacccgcg
    10681 cccacagtgg cgagtcctcg tcggcgtggc ggccgacctt ctgctcctcg tccgactcgc
    10741 cgccggcctg gtcgagctcc ttggactccg gcgacccgcg ctgcaggaac cagaccgtcg
    10801 cggtgatgta gagcgcgaac tgcaggtact ccgactgcca gttctcgagc atcgcgacgc
    10861 cgaactccga cgagagcagg aagcgcccgt agccgatcga ctcgccgtcg tggcgcagct
    10921 ggctgttgtt gaagtcctgc cagccggcga gcgactgcag cgcgagcgcg atcaggaaga
    10981 tcgccaggaa gaagaccgac aggctgttgt cgtgcaggaa cttcctcact gcccgatcag
    11041 cccgatcgcg atgtagagcg cgaggccgac ggcgatgatc gccgtgtagg tccagaacat
    11101 cgcccgcatc aggactccac ctcgcagtcg tagggcgact cgcggccggg ggcgggccgg
    11161 cagcccgcgg ccgtgaccag ccagccgtcg ccggtctcct cgacgaagac gctgtcgcct
    11221 ccgtcggtct cgaccttcgc ctcggtgatg taggcctctt ccgcggtcac gcgctggccc
    11281 gacagcttca acgcgagcac cgcctccggg cacggcttct gctcgtcctt gacgagctgc
    11341 tcgcgagtgt cgtcgctcaa cacggcgcac gcggcggccc cgtcgtcctc cgagtacgcg
    11401 ctgtagagcc gttccgccgc gtcggccgcc ccgtccgaat ccgtcgctcc acagccggtg
    11461 agcgccagcc ccacagcgag gatcagcagc gccctctgcg gtgtcgcccc catgccgctt
    11521 cccttccctc tggcatgggc gagcaaacag aagcgctttt cttggggccc gagcgctaag
    11581 gggagcagtc gacgatctcg agcaccggct cgaggccggc gaggtcaccc gcgttgcgcg
    11641 tgtagagcgg gagctggtgc gcgacggcgg tggcggcgat cagcaggtcg acggcgcgcc
    11701 ggccgcgggc cttgcggccg gtggcgacga tcgccgcata gatgcggccg taggcgcggg
    11761 cgcactcggc atcgaagggc agtggctcgt gcatggcctc gactatctgg agccgctcct
    11821 ggcgtcgccc ccgctcggcc ggcgagtcgg tcgcgtgcgg tcccgcagcg agctctgcgg
    11881 cggtcagggc cgagacggcg atctccgtcg ggaggaacgc gctgtcggcg ggagagacga
    11941 ggaccgacgt gtcgacgagc cccgcgccgc tccggacctc aggcacgggg gtcggcggcc
    12001 tggtcggcca gtgcgtcgag gtcggcgcgc aggcgagccg cgtccaccgg cggcgtgcgc
    12061 gcgaacgcgt cgagcagcgc ctcggcgccg acgaagcggc ggcggccgat cggagacagc
    12121 tcgccgaccg ccaccccgtt gcgggtgacg acgaaccgct cgccgcggtc cagcgcgcgc
    12181 atgatcgccc cgctctcgtt gcgcagctca cgttgtgtca gctcgcgcgg catggactca
    12241 gcgtagcact cgtgcgacgc tgtggatggg tcgaggagca gggtcggcat cgttcgagaa
    12301 cgccgtggtc gtatgtctcc actcgcccac cgcgcgttcg gcgcgaacct cgccttcgag
    12361 cgcaagcgcc gtgccctgtc gatgaacgcg ctcgcacgtc tcgccggaac ccacgcgagc
    12421 gagatcagcc gtctcgagcg cggtcagcgc gacccgcgac tctcgacgat ggtccgcgtc
    12481 tcgcgcgcac tcgaggtccc gctcgccgac ctgctgcgcg gggtccggta ggccgcgccg
    12541 cctgattggc cagccgggct cgcgggtaga cggtccgcca ggcaaacgac ttcaggaggc
    12601 aacgagatgg cggagctgac cccgatcgac gagaagctgg ccgaggtgct ggggctggcg
    12661 caggcgtctc aggacaccgt ctcgaaggtg ctcaagctct gcgacgacga gcaggtcgtc
    12721 gagacgctcg agcggatgcg cgacgaggcg cgcgagacgc aggagcgctg cgaggcgatc
    12781 gccgcggagc tcgacggcaa gaagaccgcg gtcgaggaga aggcccgcga gaccaagcag
    12841 gaggcgtcgc agatgcgcga cgactacctc ggcgacgacg ccgacgacct cgacggcctc
    12901 gagttcatga tcatggccga ggcgggcgag ctcggacacg tcgagatcgt cggcgcgatg
    12961 gcctagcaga ccggcgaccg gcagctccag gagctcgtcg gctgggcagc cgatccagca
    13021 gcgccattac gagcagacgc gtgagtgcgc cctcgcgctg gcgcgccagg aggcgtcgga
    13081 gtagcagccg ccggccgagt caggccgctg ggcaccgccg gagggcgccc agcgcagcgg
    13141 tcagctcgcg ttcgcgatcc ccgtgaagat gcaggcgttg ccggcgccga ggacgccgtc
    13201 gccggccggc gggctcccga ccttcgtcgc gatcgccagc tgtccgtgga tcgccacgcc
    13261 gggagcgatc acgtagaagg ggccggccga ggcgccgctg gtgttcaacg tgcccgggtt
    13321 gccgacaccg gcctggctgc tcgtgcgcag caggtccctg tcctcctcgg gagtcgctgg
    13381 cccgagcagg ccgctgccgc tgtcgccctc gtcgccggcg aagaccgctc cggcggcgga
    13441 ggtctcgacg tagatctcgc cctcgaccga cgggttcgcc ggatccgtgt tgacggcgta
    13501 gcacttgccg tagaccgtta tcggtccctc gctgagcagc gggacacgct gagccgcggc
    13561 acgtgccgcc gtctgcggcg tcgccgtcgc ggacggagcg acgcgggtgt tgaagcgcgc
    13621 aatccgcatt ccctgcagcg agtcggcgac accggccgac tgtgcccgcg tggcgttcgg
    13681 caccgagccg gcgaggacgc cgagcgcctg gcgggcgcgc gtgtcggccg cacgccccgt
    13741 ctcgagcgcc tggtcggcgg tccgcgtcgc gacgcctgcc tgcgtgccgg ccgcgcggcc
    13801 ggtcttcagc gcccggttgg cggtccgtgc cgccttgcgc gcccgcttgt cggccttctt
    13861 gccgatcttc agcgccttct tggccacctt gagcgggttg ggcgcggctc cggtggctgg
    13921 gagcgcgacg acgagggcgg ccacgatggc aagggcgatg acggcgtggg agcgtttcat
    13981 gcgcgcgcac gctaggcatg ccgtcggacg gcgcgggtgg cccgccggcg cgcctcggcg
    14041 cttgctgggc atgcggccgg attagcgcgc gtgcagcgtg ggtaggccta cctgcgactg
    14101 gccatcgaag gagaggagcg gtatgggcat tctcggatgg atcgtgctgg gactgctggc
    14161 gggggcgatc gcgaaggcga tcctgcccgg cgacgacccg gggggcatct tcgtgacgat
    14221 gctgatcggc atcgcgggcg cgatcatcgg cggcctgatc gcgagcgcgc tcgacatcgg
    14281 cgacctcgac gagttcttcg acatcggcac gtggctgatc gcgatagccg gatcgctgct
    14341 gctcctgctg gtctaccgga tggtcgtcgg aaggcgggac gacagagtca cctagccgct
    14401 cccgcgcggc gagtacggac gacagatcgt cttactcgcc gcgcagcagc gcctgcatgt
    14461 cggcgacctg gccgaggtgg gcgagcttgt cggggttcac caccgagtgg atcgcctgga
    14521 cgcggccgtc ggcgatctcg agcgtcatag cgccgaagag gcggccgtcg gcgtctagca
    14581 gcagcgcgcc cggctgcccg ttgaggtcca cccggcggac gaacggaccg ccgaagcggc
    14641 ggcccgcctt cgcccagttg acgagcgtgc gagcgacgcg gttggcgccg gagagcgcgc
    14701 gggcgagcgc ggggaccttg ccgccgccgt cgccgtgcag ggcgacgtcg tccgcgagca
    14761 gcgcctcgag gccggcgagg tctccctcgg ccgccgcggc gaagaagcgg tcggcgagcg
    14821 catcgcgccg ctcgcgcgag gcgtcgaagc gaggacggcg cgcctcgacc gccttgcgcg
    14881 cgcgggtcgc gagctggcgt gcgttgtcct cgctcttgcc gacgatcgcg gcgacctcgt
    14941 cgtaggggta gtcgaagacg tcgcgcagca gtaaggcggc gcgctgctcc ggcgtcaggc
    15001 tctcgagcag cacgaggaag gcgaacgaga gcgagtcggc cagctcggcc cgctcgggag
    15061 gcaggtcgtc ggtcgcggtg accagcggct ccggcagcca ttcgccgacg tactgctcgc
    15121 ggcgggcgcg cgcggagcgc agctggtcga tcgccaagcg cgtgacgacg gtcgagacgt
    15181 aggcctgcgg ggactcgatc cgctcgcccg cggcgcgcgc ccggtgcagc cgcagcagcg
    15241 cctcctggac cacgtcctcg gcgtcgccga cgctgccgag catccggtag gcgatcgcga
    15301 acccgcgccg gcggagcgcg tcgatctcgt cgtcctcggc ggtggccatc gccgcctcag
    15361 ccccgcgcct cgccggcgag gctctcccac atgctgcggt ggcgcggctg ccagccgagc
    15421 tcgcgcttcg ccttggcgtt cgaagcgccg cgcacctcgg tcatcatgac cgtcgcggcc
    15481 tcgcccgcag ccagccggcc gacgaaccgc gggacgcgca gcggccgctt ggcgccgatc
    15541 gcctccgcgg tcgccggcag ccactcgcgt accggtgcgg gggagtcgtc gacgacgttg
    15601 tagatcccgg gcgcgccgcg ctcgatcgcc agcgccgtcg cctcggccgc gtcggcgacg
    15661 tggacgaacg accagacgcc gccgccatcg cctacgaccg ggaacttgcg cgcccggatc
    15721 tcggcgacct gctcgccctc gggctcgagc gccatcgagg ttcccgggcc gtagaagccg
    15781 ccgtagcgca ggacgacgcc ctcgatgccg cccgcgccag tgaccgcctg ctcgaggtga
    15841 cggatcgccg cgtgggtggt gcgcagctgg cgcggcgggt cggggtcgag cggctcgtcc
    15901 tcgctcttca cgggcgcgcc gtcgcgcgcg tacggccagc cggcgaagct ctgggcgacg
    15961 aagcgccgcg ccccgaccgc gcgggcggcg gcgagcaggt ggtcggtgcc ctcggtccgc
    16021 agccggttgg tcatcgcgaa ggtccgctcg atgtggcggg cgtcgagccc gcccgcgagc
    16081 gctgtcagct ggtggaccac cacctcgggc tcggcctctg ccacagcgcg ggcgacggcg
    16141 tcgggatcga gcgcgtcggc gaccacgggg gtggcgccga gcgagcgcag gaggtcgcgc
    16201 ttggcgtccc ggcgcgtcat gcccgtgacc tcatgaccgc gctcgaccag tcgcgggacc
    16261 agctgcctgc ccagcgctcc cgtggcgccg gctacgagga tcttcatggc gagctccttt
    16321 ctcgtgggtg cttacccatc aggacggagc aggggtgacg tctgtgacag cgcggcgcgt
    16381 cgggggcggg cggctacggc ccagcacgga gccccagccg ccgcttcagc gccagcaccc
    16441 gttcggcgct ttcgcgcacg cgcgagcgaa aggcaggcga gcggcgggcg gcgcgcagca
    16501 ggcgcgggta gacgaggttc cagctgcccg agccggtcat caggacgatg tcggcgccgg
    16561 cggcgatcga gcgaacggcg gcggtcgcga cgtcggagcg gtcgaggacg gcctgcgcct
    16621 cgatcgagtc ggtgatcgcg accccgcgga agccgagctg gtcgcgcagg acgtcggtga
    16681 gcagggtccg ggactgggag gcgatgcggt ctggatcgaa ggcggggtag agggcgtgtc
    16741 cggccatcac gagcggcgcg tcggcggcga ccgcggcgcg gtagggctcg aggtcggcgg
    16801 cctcgagctc ggcgcgcggc gcgtcgatcg tggcggagcc gtcgtccgtg ttgacggtcg
    16861 cccggccgag gccggggtag tgcttcacgg tggcggcgac gccggcacgc tcgtgcgcct
    16921 ggatcgcggc cgtggtgagg gcggcgacct ggcgcgggtc tccgggatag agccggcctg
    16981 gcagcgcgcc cccgccgatc ggtggctcga cgtcggcgac cggggcgagg ttcacgttga
    17041 cgccggccga tcgcagtccg cgcgcggcct cggtcgcggc ggcccgggcc tccgccggcg
    17101 tcgccgtggc gctctgcggc gcggccggcc ccgcgaacgg cacattgcgg atctcaccgc
    17161 cctcctggtc ggttgcgatc agtgctccgc tgcgggcggc gccctggatc gaccgggtca
    17221 ggcgtcgcag ctgggtcgcg tcgacgacgt tgccgccgaa gaggatcacc ccggcgccct
    17281 cgccgtcgcg caggcggcgg cggacgtagc cgggcgccga cgtcccgtcg aaggccatga
    17341 cgagcagctg gccgacggcc tggcgcagcg gcagtgtggg gctgccggca gactgcgtcg
    17401 tgaggcctgc gccgccgccg tcatcgtcgc cgccacaggc aacggccagc acgatcgttg
    17461 ccgcgaccgt cagggccacc accgacccga gggggcggcg tcgaggcgct ggatcttgcg
    17521 ggcgcgtcat cggcgcggcg actctagcca gcgccttgga ttttcacgtc ggtcgaggcc
    17581 cgcggatccg tacgttgcca aacacgctct cagatacctc aagggtccct gtaccccgtg
    17641 gagatcggcg agtccgtgcg gtctacggtc gctctagagc caaatgtgtc cctgcggacg
    17701 ctaccccctt gacacccaga taccccagag tttaccgtct gttctcagaa actttaggtt
    17761 tccgtctaag cggtgtgagc gagcgtcgag cgcacggcac gcctgagcga gagaggactg
    17821 agttgatgtt ttcgaacttg aaggggcgct tcggcgtcgt gggcttgttg gcggtcgtca
    17881 ttgcggtggc ggtgtcggtg gcgtcggtgc cggctgtggc tgagcctgtc gcgaacgcgt
    17941 cggcgagctt ggcgaag
//
LOCUS       DATABV010000096         9042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_349951, whole genome shotgun sequence.
ACCESSION   DATABV010000096 DATABV010000000
VERSION     DATABV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349951"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..632)
                     /locus_tag="VHF88_04045"
     CDS             complement(66..632)
                     /locus_tag="VHF88_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160974.1"
                     /translation="MINDTVAETARAAHDWGLASWLGGSMFGKFALNPSVAQISDHRE
                     RGKVVNAAWNGYNLINTVSLGAVATGWFASRLTETRPDRLSNRERDLAKAKDGLTIAA
                     LVTGIASGIEGARLAKTEPEGAVPIERGTVPAEDTPKPAARIQRALGALGTANIASGV
                     GLVIVNAMLAQANHSRPPLRRALLRRSK"
     gene            complement(727..1239)
                     /locus_tag="VHF88_04050"
     CDS             complement(727..1239)
                     /locus_tag="VHF88_04050"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2160975.1"
                     /translation="MDRNDLRAVQAPLKQRYESDPEAALVTLAAQGALGEGISCSVTT
                     GRALAEAGLHPATGGDGSLLCSGDMLLEALVACAGVTLRAVATSLGIVVRSGQVRAEG
                     DLDFRGTLGVARDAPVGFAAIRLTIEVDTDADADALATLSKLTERYCVVYQTLAGGAA
                     LSTRVEPVQG"
     gene            1320..1886
                     /locus_tag="VHF88_04055"
     CDS             1320..1886
                     /locus_tag="VHF88_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2160976.1"
                     /translation="MAARIVVTEFVSLDGVMEAPGGEPFKYPGWTFEFDRGDDGNQFK
                     VEETQQSDGLLLGRRTYESFAGAWPEREGEFADRFNSMPKFVVSTTLGAADWNNTTVL
                     DSGDATGDVRRLKQEFDGELQVPGSHRLVQELIESDLVDQINLMVFPVILGTGKKVFE
                     EKPERRDLRLTESKVVGEGIAVLIYEKS"
     gene            1996..2658
                     /locus_tag="VHF88_04060"
     CDS             1996..2658
                     /locus_tag="VHF88_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX2160977.1"
                     /translation="MQPKLVVEFIGTFFLVLTVGLAVANAGDLAPLAIGAVLMVMVFA
                     GGHVSGAHFNPAISTAVYLRGKMTSTQEYGAYVVTQLVAAVVAALVVMALGFDYDEAA
                     ATAGAGKMLIAEFLFTFGLAWVVLNVATARGTEDNSFYGLAIGFTVVAGAYAVGAISG
                     GAFNPAVALGAMVMGLFSWGDIWIYAIATLLGGAVAAFVFLYTQPGEKVTGDMRAAET
                     EA"
     gene            2726..4120
                     /locus_tag="VHF88_04065"
     CDS             2726..4120
                     /locus_tag="VHF88_04065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2160978.1"
                     /translation="MSSAVPDSDLKTRHRAMWASGDYPSMVETFLLPLGPRLVDACGI
                     EAGMKVLDVAAGTGNASIPAAERGAAVTASDLTPELLDAGRQRAEADGLELEWVTADA
                     ESLPFEDASFDVVMSSIGAMFAPHHQDVADELVRVCRPGGTVGMLNWTPEGMIGALFR
                     TMGPFAPPPPPGAQPPPLWGGEQHLRELFGDRVDWRTLERDNLEITAFDRPRDYGEHF
                     KARYGPTIAARANAAKDGREGELDDALDQFCDEWNRGSDDDARFEAEYLVAVATDLLL
                     GRKRCGAEPQRLVGQQPAAVASPREAAITLQLLESSRHGRAACANEPCDRLVSQLHRH
                     DDSAGRDLSPAVGELREQHQQPRLESRGASDRARKRERMRAAHRARDECAGQHRPACR
                     GLREARAEQREARLLQHVPADEVGHQRVVGRLDPRMDDVARTEQLRARHLPDHHLAAH
                     QARHDQGPDAPIGR"
     gene            complement(4327..5136)
                     /locus_tag="VHF88_04070"
     CDS             complement(4327..5136)
                     /locus_tag="VHF88_04070"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEX2160979.1"
                     /translation="MGTAALGALTAVAGVAVAQSDKGKIVADSGQASRIEATKIARTI
                     ANRKTIKSAVFAARPPYGHVAARSTKPLTDFPLSGGSYMILSTGSALLADNKDSGPAT
                     GRNAGGPAIRGARDVTILRLNIRVPKGRNCLDLRFRFLSEEFPEFVNMEFNDAFVAEL
                     DKTTWDTRPVGDPSIETDRNFARDTEGNRISVNAVGDASVNAARAKGTTYDGATRLLR
                     ASTRVTPGGHKLYLSLFDQGDREYDSAVFIDGLSFRRSAVCENGAVLDENR"
     gene            complement(5316..6485)
                     /locus_tag="VHF88_04075"
     CDS             complement(5316..6485)
                     /locus_tag="VHF88_04075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX2160980.1"
                     /translation="MRALCWMGINDLQVNTVPDPSIVNPHDAILKVRLTTTCGSDLHL
                     VDGYIPGMREGDVIGHEYMGEVVEAGRETTRLKPGDRVVVPSFLGCGSCWYCEHDHWS
                     LCDNTNPKPELQEPVLGYPTGGIPGYTHPFGGYAGSHAEYIRVPHADVNCFQVPEGIA
                     DEQALFLSDAVPTGYMGADFCDIQPGDTVAVWGCGGVGLMAQRSALLLGAGRVIAIDR
                     LPERLALARDKVGAETIDYSETDSVLEELRELTGGRGPDACIEAVGMEAHGTGPQYAY
                     DRVKQAMHLHSDRGSALREAAMACRKAGTLAVVGVYGVIDKFPLGVLMNKGITIRTSQ
                     QPGQRYMPRMLEHVQKGELDPSFLLTHRMSLEEGPRGYEIFKEKRDGCVRAAFAP"
     gene            complement(6482..6889)
                     /locus_tag="VHF88_04080"
     CDS             complement(6482..6889)
                     /locus_tag="VHF88_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160981.1"
                     /translation="MKTKLPISKDAVLGKAQAVVAKVRPGATEGRRSLPIRRPIEDVQ
                     RLWSDEAARAAVLDGIAVAEASLQLVPDDREGAGGSLATVELRLEAAVPGMATQALAG
                     KAVRRLKALAETGEVPTTEGNPAARDDAGEETA"
     gene            7247..8518
                     /locus_tag="VHF88_04085"
     CDS             7247..8518
                     /locus_tag="VHF88_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate 2-monooxygenase"
                     /protein_id="HEX2160982.1"
                     /translation="MDGLTRQSEVYLDGVSGRRPRVPTDLTQLEDRARETMSREAFAY
                     VAGGAGTGSTMRANRAAFERQRIVPRMLRDVSARDTSVELFGRRLAAPFLLAPIGVAE
                     MAHRDADLATARAARGEGIPMIFSNQASEPMEACARELGDSPRWFQLYWSKSDALVES
                     FVGRAEACGCEAIVVTLDTTLLGWRTRDLDLGWLPFLRGMGIGQYTSDPVFRRLVAEA
                     KARPDPDAPKPRPTPAALKTLSALLRRRVSRASVQRFVEIYSRPSLSWDDLPFLRERT
                     KLPIVLKGICHPDDARRALDAGIDGVIVSNHGGRQVDGAIATLDALPGVAEAIDGRIP
                     VLLDSGVRSGADVFKALALGARAVLLGRPYIYALALAGEAGVRQVLRDVRADFDLTMG
                     LAGCASVDEIGAESLTDAGSLGAATSPARPA"
     gene            complement(8493..>9042)
                     /locus_tag="VHF88_04090"
     CDS             complement(8493..>9042)
                     /locus_tag="VHF88_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2160983.1"
                     /translation="LGGGERALAVATDVTSWDDNAALVAAALNSFGRLDVAFANAGFG
                     AKRGWLEETPEHWREMVLTNVLGAAYTVRAALPALRESRGHVLLTGSVAGRRALPGSL
                     YSCTKWAVTAMAEAVRAELVDSGIRTTLVEPGGVETPFFDNPGDGRLEADDVARAVIY
                     AVSQPPHVDVNDVLIRPVGQAT"
BASE COUNT         1253 a         3207 c         3202 g         1380 t
ORIGIN      
        1 ccccgcaagc gggggcgagc cggcgcttgg atcgcgcgcg agcggctttg ccactaggcc
       61 cctggctact tgctgcggcg cagcagagcc cgccgtagcg gcgggcgact gtggttggct
      121 tgggcgagca tcgcgttgac gatgacgagg ccgacgcccg acgcgatgtt ggcggtgccg
      181 agcgctccga gcgcgcgttg gattcgcgcg gcgggcttcg gcgtgtcctc ggcgggcacc
      241 gtcccgcgct cgatcggtac cgcaccctcc ggctccgtct tcgccagccg ggctccctcg
      301 attccgctcg cgatgccggt cacgagagcg gcgatcgtca ggccgtcctt cgccttggcc
      361 aggtcgcgct ccctgttcga aagccggtcc ggacgcgtct cggtcaggcg ggaggcgaac
      421 cagccggtgg ctacggcgcc gagcgaaacc gtgttgatca ggttgtagcc gttccatgcc
      481 gcgttgacga ccttgccgcg ctcacggtgg tcgctgatct gggccacgga tgggttgagc
      541 gcgaacttgc cgaacatgct ccccccgagc cagcttgcca acccccagtc gtgcgccgcc
      601 cgggccgtct cggcgactgt gtcgttgatc atcggaacct cctcgttgga tgggtcacgt
      661 ggggtaccca tcccgtcccg gccaaaccgt cttccaggcc agcaggctca gtggcgacgg
      721 cgggcgtcag ccctggaccg gctccacccg cgtcgacagc gcggctcccc cggcgagggt
      781 ctggtagacg acgcagtagc gctcggtgag cttgctcagc gtcgccagcg cgtcggcgtc
      841 ggcgtcggtg tcgacctcga tcgtcagccg gatggccgcg aagccgacgg gcgcgtcgcg
      901 ggcgacgccg agcgttccac gaaagtcgag gtcgccctcg gcacgcacct gcccggaccg
      961 gaccacgatc ccgagcgagg tggcgacggc gcgcagcgtc acgcccgcgc acgcgacgag
     1021 cgcctcgagc agcatgtcgc ccgagcacag cagcgagccg tcgccgccgg tcgccgggtg
     1081 cagaccggcc tcggcgagcg cgcgtccggt cgtcaccgag cacgagatgc cctcgccgag
     1141 cgctccctgg gccgccagcg tgacgagcgc ggcctccggg tcggactcgt agcgttgctt
     1201 gagcggcgcc tgcaccgccc tcaggtcgtt gcggtccatg tcgagcagcg tatgcggtcc
     1261 gcctcggccc ggactgcaag gcttaccgcc cgggaccaga ccgctgagga ggccgatcga
     1321 tggcagccag gatcgtcgtg acggagttcg tgtcgctcga cggggtgatg gaggcgccgg
     1381 gaggggagcc gttcaagtac cccgggtgga cgttcgagtt cgaccgcggt gacgacggca
     1441 accagttcaa ggtcgaggag acgcagcagt ccgacggcct gctgctcggt cgccgcacgt
     1501 acgagagctt cgcgggcgcg tggcccgagc gcgagggcga gttcgccgac aggttcaact
     1561 cgatgccgaa gttcgtcgtc tcgacgacgc tcggcgcggc cgactggaac aacacgaccg
     1621 tcctcgacag cggcgacgcg acgggcgacg tgcgcaggct caagcaggag ttcgacggcg
     1681 agctgcaggt cccgggcagc caccggctcg tccaggagct gatcgagagc gacctcgtcg
     1741 accagatcaa cctgatggtc tttccggtga tcctcgggac ggggaagaag gtcttcgagg
     1801 agaagccgga gcgacgtgac ctgcggctca cggagtcgaa ggtcgtcggc gagggcatcg
     1861 ccgtgctgat ctacgagaag tcctagagcg gttccgtcgg gcgggggcgc cagtcttcac
     1921 tcaggtcgtg cgccgaccgg cccaccggag ccaccggcgt ctctcccccg aaccaggagc
     1981 gaagcaacca tgcccatgca gccgaagctc gtcgtcgagt tcatcgggac cttcttcttg
     2041 gtgctgaccg tcggcttggc ggtcgccaac gcgggggatc tggcgccgct ggcgatcggc
     2101 gccgtgctga tggtcatggt cttcgccggt gggcacgtct cgggcgccca cttcaacccg
     2161 gcgatcagca ctgccgtcta cctgcgcggg aagatgacct cgacacagga gtacggcgcc
     2221 tacgtcgtga cccagctcgt cgcggccgtc gtcgcggcgc tggtcgtgat ggcgctcggc
     2281 ttcgactacg acgaggcggc cgcgacagcc ggcgccggca agatgctgat cgccgagttc
     2341 ctgttcacct tcgggctcgc ctgggtcgtg ctgaacgtgg cgactgcgcg cggcacggag
     2401 gacaactcct tctacggcct ggcgatcggc ttcacggtcg tcgccggtgc ctacgcggtc
     2461 ggggcgatct cgggtggcgc cttcaacccg gccgtcgcgc tcggcgcgat ggtgatgggc
     2521 ctgttctcgt ggggcgatat ctggatctac gcgatcgcga ccctgcttgg cggagcggtc
     2581 gccgccttcg tcttcctcta cacgcagccc ggcgagaagg tcacgggcga catgcgcgcc
     2641 gccgagaccg aggcataagg gtcggaggct ggacagcgcc ccgacgcggc gctacgttcg
     2701 gcgctcaccc atagaggagg ctgctatgtc atccgctgtg ccggactccg atctcaagac
     2761 gcgccatcgc gcgatgtggg cgtcgggcga ctacccctcg atggtcgaga ccttcctgct
     2821 gccgctcggg ccgcggctcg tcgacgcgtg cggcatcgag gcggggatga aggtcctcga
     2881 cgtggcagcg ggcactggca acgcgtcgat cccggccgcc gagcgcggcg ctgccgtgac
     2941 ggcgagcgat ctaacgccgg agctgctcga cgccgggcgg cagcgggccg aggccgacgg
     3001 cctcgagctg gagtgggtga cagccgacgc cgagagcctg ccgttcgagg acgcgtcctt
     3061 cgacgtcgtg atgtcgtcga tcggcgcgat gttcgcgccg caccaccagg acgtcgccga
     3121 cgagctcgtc cgggtctgcc gtccgggcgg cacggtcggg atgctgaact ggacgcccga
     3181 gggcatgatc ggcgcgctgt tccgcacgat ggggccgttc gcgccgccac caccgcccgg
     3241 cgcgcagccg ccgccgctgt ggggaggcga gcagcacctg cgcgagctgt tcggcgaccg
     3301 ggtcgactgg cgcacgctgg agcgcgacaa cctggagatc accgccttcg accgcccgcg
     3361 cgactacggc gagcacttca aggcccgcta cggcccgacg atcgccgcgc gcgcgaacgc
     3421 cgccaaggac ggccgcgagg gcgagctcga cgacgcgctc gaccagttct gcgacgagtg
     3481 gaaccgcggc agcgacgacg acgcgcgctt cgaggcggag tacctcgtcg ccgtcgcgac
     3541 cgacctgcta ctcgggcgta agaggtgcgg cgccgagccg cagcggcttg tcggtcagca
     3601 gccagccgcc gtggcctcgc cgcgcgaggc ggccatcacg ctccagctgc ttgagagcag
     3661 tcgacacgga cgggcggcgt gcgccaacga gccttgcgat cgtctcgtga gtcagctcca
     3721 ccggcacgac gactccgccg gccgtgacct ttccccagcg gtcggcgagc tgcgagaaca
     3781 gcatcagcag ccgcgtctcg agtcccgtgg tgcaagcgat cgcgctcgca agcgcgagcg
     3841 catgcgagcg gcgcaccgtg cgcgcgacga gtgcgctggt cagcaccggc cagcgtgccg
     3901 cggtctccgc gaagcgcgcg ccgagcagcg cgaggcgcga ctcctccagc acgtgccagc
     3961 ggacgaagta gggcaccagc gcgtcgtcgg ccgcttggac ccacggatgg atgacgtcgc
     4021 ccggaccgag cagctccgcg ctcgccacct ccccgatcac cacctcgcgg cacatcaggc
     4081 ccgacacgat cagggccccg acgcgcccat cggtcgttga gtcgccttcc gcgcgccggg
     4141 gggtccagtc gccgcgctcc accgtgacgg ccggcgcgac gagcgccagc cgcgcgtcgc
     4201 gccggtcctc ctcgggtagg acttccgcca ggtcgggatc ggcgtccagg aggcgcacga
     4261 tgtccatgag ggggacggta cagacaactc ccgtgccgat tcacaggcgg aatgagtcct
     4321 actcggctat cggttctcgt ccaacaccgc gccgttctcg cagacggccg acctccggaa
     4381 gctcaacccg tcgatgaaca ccgccgagtc gtactcacgg tcgccctgat cgaagagcga
     4441 taggtagagc ttgtgcccgc cgggcgtgac ccgagtcgat gcgcgcagca gccgcgtcgc
     4501 cccgtcgtag gtcgtgccct tggctcgggc ggcgttgacg ctggcgtcgc cgaccgcgtt
     4561 gacgctgatc cggttgccct cggtgtctcg cgcgaagttg cggtcggtct cgatcgacgg
     4621 atcgccgacg ggacgtgtgt cccacgtggt cttgtcgagc tcggcgacga aggcgtcgtt
     4681 gaactccatg ttgacgaact ccgggaactc ctcgctgagg aagcggaagc ggaggtcgag
     4741 gcagttgcgc cccttgggca ccctgatgtt cagccgcagg atcgtgacgt cgcgggcgcc
     4801 gcggatcgcc gggccgccgg cattcctccc ggtcgcgggc ccggagtcct tgttgtccgc
     4861 gagcagcgcg gagccggtgc tgaggatcat gtaggagccg ccggagagcg ggaagtcggt
     4921 cagcggcttg gtcgaccggg ccgcgacgtg gccgtacggc ggccgggcgg cgaagactgc
     4981 actcttgatc gtcttgcggt tggcgatcgt ccgcgcgatc ttcgtcgcct cgatacggga
     5041 cgcctgcccc gagtcagcaa cgatcttgcc cttgtccgac tgcgccaccg caacgcccgc
     5101 gacggcggtc agcgccccca gcgctgccgt ccccacaccg atcctgcgcc tcgcccgtcc
     5161 ccacatctcg caaacctccg ttcacgcgag gccgtgacct cggcgtcttg acatcgtcgt
     5221 aggtgtcgct gtccgtctgc gacgcggctc acacgcggct tgtcaccgcg ctgacagctg
     5281 aggtttggcg ccggactgtc ctccgcgggc cggacttagg gcgcgaacgc tgcccgcacg
     5341 cagccgtccc gcttctcctt gaagatctcg tagccgcgcg ggccctcctc gagcgacatc
     5401 cggtgggtga gcaggaacga ggggtcgagc tcgcccttct ggacgtgctc gagcatccgt
     5461 ggcatgtaac gctgccccgg ctgctgcgag gttcggatcg tgatcccctt gttcatcagc
     5521 acgccgagcg ggaacttgtc gatcaccccg tagacgccga cgaccgcgag cgtgccggcc
     5581 ttgcggcagg ccatcgccgc ctcgcgcagc gcgctgccgc gatcgctgtg caggtgcatc
     5641 gcctgcttga cgcggtcgta ggcgtactgc ggcccggtcc cgtgggcctc catgccgacc
     5701 gcctcgatgc aggcgtccgg cccacggccg ccggtcagct cgcgcagctc ctcgagcacg
     5761 ctgtcggtct cgctgtagtc gatcgtctcg gcgccgacct tgtcgcgcgc gagcgccagg
     5821 cgctcgggca gccggtcgat cgcgatcacg cggccggcac ccagcagcag ggcgctgcgc
     5881 tgggccatca gcccgacgcc gccgcagccc cagaccgcga cggtgtcgcc cggctggatg
     5941 tcgcagaagt cggcgcccat gtagccggtc gggacggcgt cggagaggaa cagcgcctgc
     6001 tcgtcggcga tgccctcggg cacctggaag cagttgacgt cggcatgcgg cacgcggatg
     6061 tactcggcgt gcgagccggc atagccgccg aaggggtgcg tgtagcccgg gatgccgccg
     6121 gtcgggtagc cgagcaccgg ctcctgcagc tcgggcttcg gattcgtgtt gtcgcatagc
     6181 gaccagtggt cgtgctcgca gtaccagcac gatccgcagc cgaggaagga cggcacgacg
     6241 acccggtcgc ccggcttcag cctcgtggtc tcacgtccgg cctcgacgac ctcgcccatg
     6301 tactcgtggc cgatcacgtc gccctcgcgc atgcccggga tgtagccgtc gacgaggtgc
     6361 aggtccgagc cgcaggtcgt cgtgagccgc accttgagga tcgcgtcgtg ggggttgacg
     6421 atcgaggggt cggggacggt gttgacctgc aggtcgttga tgcccatcca gcacagcgct
     6481 ctcatgccgt ctcctctccg gcgtcgtcgc gcgccgccgg gttgccctcg gtggtcggaa
     6541 cctcgccggt ctcggcgagc gccttcagtc gtctcaccgc cttgccggcc agcgcctggg
     6601 tcgccatgcc cggcactgcc gcctcgagcc gcaactcgac cgtcgcgagc gagcccccgg
     6661 caccctcgcg gtcgtcaggg acgagctgca gagacgcctc ggcgaccgcg atgccgtcga
     6721 ggaccgccgc ccgcgccgcc tcgtcgctcc acaagcgctg cacgtcctcg atcgggcgac
     6781 ggatcggaag cgaccgccga ccctcggtcg ccccggggcg gaccttcgca actacggcct
     6841 gagccttgcc cagcactgcg tctttgctga tcggaagctt ggtcttcatt ccggcccgtt
     6901 acccgtcagg ccgaccctca accggggaga tcgaggcgcc ttactgcggc ggtgtcaccg
     6961 ctcgagacgc caccgctgac acgtctccag atcccatctg gctcgcctgc gcgtcggtcg
     7021 aagcggctac gtcgagcggc cggtcggacg ccggctcgtc ggacgacgac atcggctcgc
     7081 tcggggcccg gggtcgggtc gtctccgtct tcgtgggcct agtcggccct acgaacctca
     7141 ccagtctccg catcgctgcc ccttcgagtc gctccctagc ggtcccctac ccacagcaag
     7201 ctgtctgaaa acgacaatcc gcacgaacgg tggtagaacc tcgcgggtgg acgggctgac
     7261 gcggcagagc gaggtctatc tggacggcgt ctccggacgg cgaccgcgcg ttccgaccga
     7321 cctcacccag ctcgaggacc gcgcgcgcga gacgatgagc cgtgaggcgt tcgcctacgt
     7381 cgcgggagga gccgggacgg gctcgacgat gcgcgcgaat cgcgcggcct tcgagcggca
     7441 gcggatcgtg ccgcggatgc tgcgcgacgt gtcggcgcgc gacacgagcg tcgagctgtt
     7501 cggtcgccgg ctggcggcgc cgttcctgct cgcgccgatc ggcgttgccg agatggccca
     7561 ccgcgacgcc gacctggcga cggctcgcgc cgctcgcggc gaggggatcc cgatgatctt
     7621 ctccaaccag gcgtccgagc cgatggaggc ctgcgcgcga gagcttggcg acagcccgcg
     7681 ctggttccag ctctactgga gcaagtcgga cgcgctggtc gagagcttcg tgggaagggc
     7741 ggaggcgtgc ggctgcgagg cgatcgtcgt cacgctcgac acgacgctgc tcggctggcg
     7801 gacgcgcgac ctcgatctcg gctggctgcc gttcctgcgc gggatgggga tcggccagta
     7861 cacgagcgac ccggtcttcc gccgtctcgt cgcggaggcg aaggcgcgtc cggaccccga
     7921 cgcgccgaag ccccgtccga cgccggcggc gctcaagacg ctgtccgcgc tgctgcggcg
     7981 gcgcgtctcg cgggcgtcgg tgcagcgctt cgtcgagatc tactcgcggc cgtcgctgag
     8041 ctgggacgac ctgccgttcc tgcgcgagcg gacgaagctg ccgatagtgc tcaagggaat
     8101 ctgccacccc gacgacgccc gccgcgcgct cgacgccggg atcgacggcg tgatcgtctc
     8161 caaccacggc ggtcggcagg tcgacggcgc tatcgcgacg ctcgacgccc tgcctggtgt
     8221 ggcggaggcg atcgacggcc gcatccccgt gctgctcgac agcggcgtgc gcagcggcgc
     8281 cgacgtcttc aaggcgcttg cgctcggcgc gcgtgccgtc ctgctcggcc ggccctacat
     8341 ctacgcgctc gcgctcgcag gcgaggcggg tgtgcgccag gtgctacgcg acgtgcgagc
     8401 ggacttcgac ctgacgatgg ggctggccgg ctgcgcctcg gtcgacgaga taggcgccga
     8461 gtcgctgact gacgccgggt cgctcggcgc cgctacgtcg cctgcccgac cggcctgatg
     8521 aggacgtcgt tgacgtccac gtgcggcggc tgcgagacgg cgtagatcac ggcgcgtgcg
     8581 acgtcgtcgg cctcgaggcg gccgtcgccg ggattgtcga agaagggcgt ctcgacgccg
     8641 ccgggctcga ccagcgtggt cctgatgccg ctgtcgacga gctccgcccg caccgcctcg
     8701 gccatcgccg tgacggccca cttcgtgcag gagtagaggc tgcccggcaa tgcccggcga
     8761 ccggcgaccg agccggtcag cagcacgtgg ccgcgcgact cgcgcagcgc cggcagcgcc
     8821 gcgcgcaccg tgtaggcggc gccgagcacg ttggtcagga ccatctcgcg ccagtgctcc
     8881 ggcgtctcct cgagccagcc gcgcttggcg ccgaagccgg cgttggcgaa cgcgacgtcg
     8941 aggcgcccga acgagttgag ggccgccgcc accagtgccg cgttgtcgtc ccagctcgtc
     9001 acgtctgtgg ccaccgcgag cgcgcgctcg ccgcccccaa gc
//
LOCUS       DATABV010000097         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2197210, whole genome shotgun sequence.
ACCESSION   DATABV010000097 DATABV010000000
VERSION     DATABV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197210"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VHF88_04095"
     CDS             complement(<1..852)
                     /locus_tag="VHF88_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX2160984.1"
                     /translation="MEPLPIPAGRALALGAVQGPTELLPVSSSAHLTLIPWLGRWGWE
                     RRDPELRKSFEVALHAGAAAALVVGQRRVIADEMRSFDARKASVLALSFAPAAAVGLA
                     FERQIERRLGGPVPTALGLLGGAAAMVAADRVPQRRLSDRAGLRDGLALGLAQAAALV
                     PGVSRNGATLSAARWRGFDRRHANFLSRTVALPVIVGAAVLKSARLRRRGLPAAQRPA
                     FAAGTAASFASTLASQALIRVVERDSALWPYAAYRAGLAAVVLGEHVRRRRAERAQAA
                     ATVVAGNG"
     gene            complement(870..1541)
                     /locus_tag="VHF88_04100"
     CDS             complement(870..1541)
                     /locus_tag="VHF88_04100"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2160985.1"
                     /translation="MTDFIVDPLVEFCVDVIGEIGYLGIFLLMLVESANIPMPSEATM
                     LFAGFKVEEGDLTLLGITAAGVLGNVVGSWGGYAMGYYGRTEILEKYPIFHVSPKQMK
                     RVEEWFERYGAATVFFTRMLPIVRTFISTPAGAARMPLGRFTLYTAAGCVPWVLLLGV
                     IGVEVGERWEEWRDKLHYFDYVVLAALVALAVWALIRWRRGGPGEGKPSKRGGRDATP
                     AETEA"
     gene            1621..>3091
                     /locus_tag="VHF88_04105"
     CDS             1621..>3091
                     /locus_tag="VHF88_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX2160986.1"
                     /translation="MDRLMDPVYRHRAAQLAIDACLVALAFYAAFRLRFLETEGGVPD
                     HYREMLWGSIGFVVVGKLVVFWGFGLYEKWWRYFRLPDYFDVFKAVAVSSAIIAIALW
                     LFRPFDDPIPRSVVVTDFLLTLVLVGGARLLVRMVVERPDRHRRSGAGERSVLVVGAG
                     SGGQMVVREMQLNPNLGNRAIGYLDDDPRKRGMRLHGVKVLGSTDEVGQVLDDTRPDE
                     VTIAIPSAPGELRGRVVAACRDRGIAVRTLPTVFELLRGGVQLTRQLREVQVEDVLGR
                     EPIRMELDRVGAYLQEKVVLVTGAGGSIGSELVRQIARVRPKLIVMLDHAEDNLFRID
                     REMIEERHFTRVESVLADCKEGDRMLEAMQRFHPDVVFHAAAYKHVPLMEGNPLEAVR
                     NNAIATRVVAETAAASGCERFVLVSTDKAVNPRTVMGASKAMAEWIVEAARHKHPQTR
                     FITVRFGNVLASSGSVVPIFRAQIERGGPVTVTDPEMTRY"
BASE COUNT          451 a         1129 c         1088 g          423 t
ORIGIN      
        1 gccgttgccg gcgacgacgg tcgccgcagc ctgggcgcgc tcggcgcggc gacggcgcac
       61 gtgctcgccg aggacgacgg cggcgagacc ggcccggtag gcggcgtagg gccacagcgc
      121 gctgtcgcgc tcgacgaccc ggatcagcgc ctgcgaggcg agcgtcgagg cgaacgaagc
      181 ggcggtcccg gcggcaaacg ccggtcgctg cgcggcgggg agcccgcgcc gtcgcaggcg
      241 cgcgctcttg agcacggcgg cgccgacgat caccggcagc gcgaccgtgc gcgagaggaa
      301 gttcgcatgg cggcggtcga acccccgcca gcgggcggcg ctgagggtcg cgccgttgcg
      361 cgagaccccc gggacgagtg cggcggcctg ggcgagcccg agcgcgagcc cgtcgcggag
      421 cccggcgcgg tcgctgaggc ggcgctgcgg gacgcggtcg gcggctacca tcgccgccgc
      481 tccgccgagc aatccgagcg ccgtcggaac cggcccgccg aggcggcgct cgatctggcg
      541 ctcgaacgcg agcccgaccg ccgccgcggg cgcgaacgac agcgccagca ccgacgcctt
      601 gcgcgcgtcg aaggagcgca tctcgtcggc gatcacccgc ctctgtccga cgacgagcgc
      661 ggccgcagcg ccggcgtgca gtgcgacctc gaagctcttg cgcagctccg ggtcccggcg
      721 ctcccatccc cagcgcccga gccaggggat cagcgtcagg tgagccgagc tcgagacggg
      781 gagcagctcc gtcgggccct ggaccgcccc gagcgcaagc gcccggccgg ccgggatcgg
      841 cagcggctcc atcgggcggg gccccgcccc tacgcctccg tctcggcggg cgtggcgtcg
      901 cggccgccgc gcttcgacgg cttgccctct ccgggccccc cacgtcgcca ccggatcagt
      961 gcccagaccg cgagcgcgac cagggccgcg agcacgacgt agtcgaagta gtggagcttg
     1021 tcgcgccact cctcccaccg ctctccgacc tcgacgccga tcacaccgag cagcagcacc
     1081 cagggcacgc acccggctgc cgtgtacagc gtgaaccggc cgagcggcat ccgcgccgca
     1141 cccgcgggcg tggagatgaa ggtgcggacg atcgggagca tccgggtgaa gaagacggtc
     1201 gcggctccgt agcgctcgaa ccactcctcg acacgtttca tctgcttcgg gctgacgtga
     1261 aagatcgggt acttctcgag aatctcggtc cgcccgtagt agcccatcgc gtagccgccc
     1321 caggagccga cgacattgcc gagcacccct gctgcggtga tcccgagcag cgtcaggtcg
     1381 ccctcctcga ccttgaaccc agcgaacagc atcgtcgcct cgctcggcat cgggatgttc
     1441 gcgctctcga cgagcatcag caggaagatg ccgaggtagc cgatctcgcc gatcacgtcg
     1501 acgcagaact cgaccagggg gtccacgatg aagtcggtca tgagaacgcg gcatggtagg
     1561 cgacggcgat gagaacggat tcccgccccc ccgccgagcg cgactagact cggtgcccgc
     1621 gtggatcgtc tcatggaccc cgtctacagg caccgcgcgg cgcagctggc gatcgacgcc
     1681 tgtctcgtcg cgctcgcctt ctacgccgcc tttcggctgc gcttcctcga gactgagggt
     1741 ggcgtccccg atcactaccg cgagatgctc tggggatcga tcgggttcgt cgtcgtcggc
     1801 aagctcgtcg tcttctgggg cttcggcctg tacgagaagt ggtggcgcta cttccgcctc
     1861 cccgactact tcgacgtgtt caaggcggtc gcggtatcga gcgcgatcat cgcaatcgcc
     1921 ctctggctgt tccgcccgtt cgacgacccg atcccccgct cggtcgtcgt caccgacttc
     1981 ctcctgacgc tggtcctagt cggcggcgcc cgcctgctgg tgcggatggt cgtcgagcgc
     2041 cccgatcgcc accgccgcag cggagcgggc gagcgcagcg tgctcgtcgt cggcgccggc
     2101 tcgggcggcc agatggtcgt ccgcgagatg cagctcaacc cgaacctcgg caatcgcgcg
     2161 atcggatacc tcgacgacga cccgcgcaag cgcgggatgc ggctgcacgg cgtcaaggtc
     2221 ctcggctcga ccgacgaggt cggccaggtc ctcgacgaca cccggccaga cgaggtcacg
     2281 atcgcgatcc cctcggcgcc cggcgagctg cgcggcaggg tcgtcgcggc ctgccgcgat
     2341 cgcgggatcg cggtgcggac gctgccgacg gtgttcgagc tgctgcgcgg tggcgtccag
     2401 ctcacccggc agctgcgcga ggtccaggtc gaggacgtgc tcggccgcga gccgatccgg
     2461 atggagctcg accgcgtcgg cgcctacctc caggagaagg tcgtcctcgt cacaggcgcc
     2521 ggcggctcga tcgggtcgga gctcgtcagg cagatcgccc gggtgcggcc gaagctgatc
     2581 gtgatgctcg accacgccga ggacaacctg ttccggatcg accgcgagat gatcgaggag
     2641 cgccacttca cccgggtcga gtcggtgctc gccgactgca aggaggggga ccggatgctg
     2701 gaggcgatgc agcgcttcca tccggacgtc gtcttccacg ccgccgccta caagcacgtg
     2761 ccgctgatgg agggcaaccc cctcgaggcg gtgcgcaaca acgcgatcgc gacccgcgtc
     2821 gtcgccgaga ccgccgccgc ctccggctgc gagcggttcg tgctcgtctc caccgacaag
     2881 gcggtcaacc cgcggacggt gatgggggcg tcgaaggcga tggccgagtg gatcgtcgag
     2941 gccgcccgcc acaagcatcc gcagacgcgc ttcatcacgg ttcgcttcgg caacgtcctc
     3001 gcctcgtcgg gcagcgtcgt cccgatcttc cgcgcccaga tcgagcgcgg cgggccggtc
     3061 acggtcaccg acccggagat gacgcgctac t
//
LOCUS       DATABV010000098        25637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1635019, whole genome shotgun sequence.
ACCESSION   DATABV010000098 DATABV010000000
VERSION     DATABV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635019"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VHF88_04110"
     CDS             complement(<1..879)
                     /locus_tag="VHF88_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX2160987.1"
                     /translation="MPQHDVLVIGAGLAGQRAALAAAEGGASVGIISKVHPVRSHSNA
                     AQGGINAALNPEDSWESHAFDTVKGSDYLGDQDAIEIMCREAPDEILHLEHLGVTFHR
                     NEQGKLGTRAFGGASAARTYYVADITGQAILHVLYEQLMKHDEVERFEEWFTTALVKD
                     ADGRIAGAVTRNIRDGSIELFTAKTVILATGGNGQIFDPTTNALICTGDGIAQAYRAG
                     AALMDMEMVQYHPTTLAGIGLLITEGARGEGAHLYNAQGERFMEKYAPNKMELASRDV
                     VSRAEQTEINEGRGFPD"
     gene            complement(949..1209)
                     /locus_tag="VHF88_04115"
     CDS             complement(949..1209)
                     /locus_tag="VHF88_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160988.1"
                     /translation="MTEQSIGGLPEAVRPSRTVASVMANDSADAESSLRVSADEPLEA
                     LLGREGLVRLGALMAVDGDGQLRGVITADDVRRALQGSSVVV"
     gene            complement(1206..2117)
                     /locus_tag="VHF88_04120"
     CDS             complement(1206..2117)
                     /locus_tag="VHF88_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2160989.1"
                     /translation="MFGGGSIQVARVFGIRIGVDPSWFFVLFLIIWSLSGYYEDRFPQ
                     GSTAFALAVVSALLFFLSVLLHELGHAFEARRSGIGISGIDLWMFGGVAKMERDSKSA
                     GEEFRVAAAGPLVTLLIAAACWLAAAAIAGSHEATHAVGFDASGDDETLAVLGYLGFV
                     NLALLLFNLIPGFPLDGGRIARAIAWKATGSRNRATRFAGTLGRGFAYLMIGAGILFA
                     LTGAIVSGIWLGIIGVFLNQAARGAIAQTELTSRIEGVSVADVMDAEPVAIPAGLTVD
                     RASTSTSCATAGPGSRSSRPTAGSSGW"
     gene            2182..3402
                     /locus_tag="VHF88_04125"
     CDS             2182..3402
                     /locus_tag="VHF88_04125"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX2160990.1"
                     /translation="MAKIEVKNPIVELDGDEMTRIIWAFIKEQLILPYLDVELEYYDL
                     GIESRDLTSDQITVDAANAIKRHGVGVKCATITPDEARVEEFGLKEMYRSPNGTIRNI
                     LGGVIFREPIVISNIPRLVPGWTKPIVIGRHAFGDQYRATDLVVPGEGSLTLTYTPRN
                     GDDPIELDVYDFPGGGVAMAMYNLDDSIRDFARASMRYGLERGYPVYLSTKNTIMKRY
                     DGRFKDLFQEIYESEFKADFDAAGITYEHRLIDDMVAAALKWEGGYVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHFRQHQQGKPTSTNPIASIFA
                     WTRALAARGRMDETPTVSEFAETLERVCVETVENGQMTKDLALLVGPDQPFLTTQEFL
                     AAIDGRLEQAMTSR"
     gene            3440..4426
                     /locus_tag="VHF88_04130"
     CDS             3440..4426
                     /locus_tag="VHF88_04130"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF003916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX2160991.1"
                     /translation="MSNPVKVTVTGAAGQIGYALLFRIASGQLLGPDVPVELRLLEIP
                     QAIKAAEGTAMELDDCAFPLLAGVEISDDPNRAFDGVNIALLVGARPRSKGMERGDLL
                     EANGGIFRPQGQAINAHAADDVKVLVVGNPANTNALIAMSNAPDVPRERFTAMMRLDH
                     NRAISQVASKLGRPVSDFTNMTVWGNHSATQYPDLFHAQVDGQSVAEAVGDQAWIEEQ
                     FIPTVAKRGAAIIEARGASSAASAANAAIDHVYDWVNGTPDGDWVSMAVPSDGSYGVA
                     EGIVSSFPCTTVDGQWSIVQGLEIDDFSRQRIDASVSELSEEREAVKGLGLI"
     gene            complement(4499..4906)
                     /locus_tag="VHF88_04135"
     CDS             complement(4499..4906)
                     /locus_tag="VHF88_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160992.1"
                     /translation="MTDFLKKTLKDIEARRRELAPLVEEYRQLEEAAAALQGVDGNGG
                     GGRSRTATSAPSRPARRRRSSGSTGSGRRGRPRGSGKRAQQALDLVAGQPGITIPELA
                     KAMKIQPNYLYRVLPGLEQDGKVKKKDKGWYPA"
     gene            complement(5064..5501)
                     /locus_tag="VHF88_04140"
     CDS             complement(5064..5501)
                     /locus_tag="VHF88_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2160993.1"
                     /translation="MKVERSIDIAASPERLYDVIMDPNCLERWVTIHAALEHAPAGPL
                     ERGSELTQRLQLAGRGFTVHWKVIEAQPARKVVWEGSGPMRSHAGVTYELTPNAGGGT
                     RFTYTNEFALPGGPLGRFAGPVVRRVTAGELDQSLDRLRAMVE"
     gene            complement(5498..6421)
                     /locus_tag="VHF88_04145"
     CDS             complement(5498..6421)
                     /locus_tag="VHF88_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX2160994.1"
                     /translation="MELVTGATGYVGGRLVQRLRSEGRAVRALARDSVRVQPLEGVEI
                     AQGDLLDGTGVRAALEGCSVAYYLVHSMEAVAEGEHDFATRDRRAAERFAADASAAGV
                     ERVVYLGGLAPANGPVSPHIASRLEVERILLDAAPGATALRASILIGARSASFRILVR
                     LVERLRLLPLPAWRDNVTRPIAERDAIEFLARTASLPATAGRSLDIAGPDMVSYGEMI
                     ERIAEQMGVGRTPVGLGVSLTPPASAVVAAITGQPLELVRPLMESLEHDLLPRNALAA
                     PQLYGIKPLPLERAIDRALAEWESFEELAAW"
     gene            6644..6838
                     /locus_tag="VHF88_04150"
     CDS             6644..6838
                     /locus_tag="VHF88_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160995.1"
                     /translation="MKKKAKRVSCEDCFFQKNQLCALELEEPCTTFRPAERNLAPEKQ
                     LAFSFRTERTWSAYAFPQPG"
     gene            6963..7757
                     /locus_tag="VHF88_04155"
     CDS             6963..7757
                     /locus_tag="VHF88_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2160996.1"
                     /translation="MRLTVLGKSPAWQDIDGACSGYLLQEGDTAVLLDCGNGVFGKLR
                     KFVDYVDVDAVVISHPHADHFIDLVPYSYALIHAPRQQPVPVPPWPGTESPARPRLIV
                     PPGARETFRKVVGAWGNEDLIEQAFELEEYAVGTVVEVGPLRFDFAVMPHFVDAHAVS
                     VTSSNGGGRLAFSADSRPAQELVEFARGADVFLVEATLPRPERSGVRGHLTAEEAGEH
                     GRDAAVGRVVLTHISDELDLEQAVERAAEAFGGPVEVAREGAAYEI"
     gene            7787..8230
                     /locus_tag="VHF88_04160"
     CDS             7787..8230
                     /locus_tag="VHF88_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2160997.1"
                     /translation="MSPERDLFANFERMRREIDELFGDVFERAGLRGRGFSPSVDVYY
                     VDDPPRAVVRADLAGVDIADVALEVRGRQLLIAGERRTGQAENRLYQQIEIEHGHFRR
                     TVELGADVVADQARASYEDGVLVVEVPLARPETAVRRIAIREASE"
     gene            8233..10611
                     /gene="lon"
                     /locus_tag="VHF88_04165"
     CDS             8233..10611
                     /gene="lon"
                     /locus_tag="VHF88_04165"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX2160998.1"
                     /translation="MTPTIEIPTPDGRTQEIPVGATRLPEALPVLPLKDSVPFPEMLT
                     PLAVGQPRSVQLVNDVLGGNRMLVMVASRDGEVENPGPEQLYSVGVVGVVARMLKVPD
                     GSLRILVQGGQRVELGDTVQRDPYLIARIVAAPDVLEPSPELEALSRNVQQTFTQIVE
                     QVPYLPEELQLAIANVDDPAELAHMIAGSLRIKTEERQELLEERDVAKRLRRLSELLA
                     REAELISIGSKIQSQVESEIDKGQREFFLRQQLKAIQEELGETDEAAAEAAELRERIE
                     AAGLPEHAAKQAERELSRFERLPPQSAEHGTIRNYLEWLADLPWSKSTEDHIDLEAAR
                     EVLDRDHYDIEKVKDRILEFLAVRKLKPDAPSSILCFVGPPGVGKTSLGRSIAAAIGR
                     EFERISVGGVRDESEIRGHRRTYVGAMPGTIVRALRDAGSNNPVLMIDEIDKMGADFR
                     GDPSSAMLEVLDPEQNGSFRDHYLDLPFDLSNVLFITTANQLEPIPGPLRDRMETIQL
                     AGYTEQEKLEIAKRYLVPRQIERTGLMKSKIEFTDPALRELIDGYTREAGVRSLEREI
                     GSVCRKVAREFAEGTRRTKRRIGPKAVGELLGRRRFHSDVGRRTDEPGVATGLAWTPV
                     GGDVLFVEATAFPGEGKLQITGQLGDVMRESASAALSYVRRNLARIAPDAPADWFAKN
                     DLHVHVPAGAIPKDGPSAGITMATALASLLSGRPVRSDTAMTGEITLTGQVLPIGGLK
                     EKALAAQRAEITRVIAPRRNEGDLEEFPPNLLGGMQFVFADTVDDVLAAALD"
     gene            10656..11126
                     /locus_tag="VHF88_04170"
     CDS             10656..11126
                     /locus_tag="VHF88_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2160999.1"
                     /translation="MAAKKKAAKGAAGAIAAGQALRDNPYVQRLVEDDDLRDELRSAF
                     DSARKAYDRIDSKGPAKALENKKVQRDLKEAAESLKSAADSLREPRKGKHGFRRLLML
                     AVIGAVLALVLSEDLRKKALDALFGAEEEYEYTSTTTPTPAAVPNGAETAANAS"
     gene            11170..11775
                     /locus_tag="VHF88_04175"
     CDS             11170..11775
                     /locus_tag="VHF88_04175"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161000.1"
                     /translation="MEASVAAPRKLEGEKAQRIVEAMRASVGKRGAAGSTFDVVAREA
                     GVSRGLLHYYFGSKERLMVEVVRSDCDQRVAALEENLATATSADEFVAFLVQTLEAFL
                     SDEPSQAVLYEMLSASRRSEEIRAELAELYRRWRGQLAAALRDKQEEGVVSLGSEPET
                     VAALLFALGDGMGLQVISDPEWDSAPAFELGFQTARRLLGA"
     gene            11935..14130
                     /locus_tag="VHF88_04180"
     CDS             11935..14130
                     /locus_tag="VHF88_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2161001.1"
                     /translation="MLGAWVALLLVSLPFAMKQTEHLTSGGFTIPGSGSEAVDHALAD
                     FDDAQRQTLSVVVARRPGGDAADVRRELARVDTIADGMAHAELTPRALTAASRDADRS
                     PIAVAPLKTVGDMNDAADLAVDLRKEIGIGEGPRNGVETYLVGEQALWAGMQDLSKKD
                     LQRAEATGFPIVLLILLAVFGSLVAAMLPLVLGFVSVMVTGAGIFFVSQATDMSVFVT
                     NVASMIGIGVAVDYSLFILSRYREEIRAGADPVDARRIAMRTSGVAVAFSGITVIVSL
                     AGLLLVDSTTLRSMAYGAIMVVAVSIVAAMTFLPALMAATGERGYRPGRTAGVVAAIV
                     SGLRRLTRRPPRPEGAPGFWQTWTDRVTRRPLVTALAASALMLTLAIPALSLSTGDGA
                     LRQFPEGNETRVGAELAAKKLGPGAASPVEVLATFAKGKATDRSNQQALTSFVGAAKQ
                     DPQVAAIRPPVPSEDGGAAMIALTPRSDAESQRARDLVDRLRDGESGAGALMNVARVD
                     VGGATAAVEDFRAQVTGSLWKIALFVLAFSYVVLFFLLRSVLLPLKAVLMNLLSVGAA
                     YGVLVAVFQWGWIDGFLGFQSLGYINTMTPPLLLAIVFGLSMDYEVFLLSRIKERYAA
                     TGDSKVAVAQGLARSAATISSAALIMVAVFAVFAGVGVPSVKEIGVGLAVAVALDATI
                     VRLVLVPATMEIMGDWNWWLPRPLDRVMPDADFEASESPAPALVGSRSG"
     gene            complement(14015..15184)
                     /locus_tag="VHF88_04185"
     CDS             complement(14015..15184)
                     /locus_tag="VHF88_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2161002.1"
                     /translation="MRLLPDPEQLVDSAATAWDLYVGPGVADLRPTPSSIVHEGPQCT
                     VRRFRSRRRSRADRAPVLLIPPLAAPASCFDLQRGCSLAEHLISLGYPTYVVDYGPIS
                     FSDRALGLEHWVEDVLPTAIEVVDEDSGGQPVQLVGWSLGGILTLLTVASDQELPVAS
                     AAVIASPFDFTRVRLAAPLRQIGRLTGGALESALYRALGGAPAPLVSRVFQLTSIDRY
                     LTKPLWVLRNLPDRDSIAHSQAIDSYMANMLAYPGRTFGQLYHRFFRVNDLADGVVDL
                     GDHQIELANVRQPVLVIASEDDVLAPAGSVEAVVDLLPNAASVTYRLVTGSHLGALTG
                     RGAEATTWRHLDEFLATASRSATRQAQGRETPTPRSRRLASRDRAGAAATSSSRP"
     gene            complement(15211..16068)
                     /locus_tag="VHF88_04190"
     CDS             complement(15211..16068)
                     /locus_tag="VHF88_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2161003.1"
                     /translation="MQPLFEHRLELAGFETRALELEGSGPPLILLHGFADSADTWRTL
                     LDRLGRGDRRAVAFDLPGFGTADPLRPDEPVLAQLTAFAKAAVQHHAPKGGAIVCGNS
                     LGGCLSLLLAERRGLRLAGIVPVAPAGLDMARWLTLIEREPVLRALLASPLPVPPQVL
                     QRVVLEIYKRLVFHHPEAIEPLIGRAFTSHFRDRRTTAQILGAARRMLPELRDPFELG
                     RIGCPVRLVWGDHDAMVFKSGAERVLESVPDAELTLIEDCGHCPQIEAADRLAELLLD
                     FPGSLAHAA"
     gene            complement(16117..17490)
                     /locus_tag="VHF88_04195"
     CDS             complement(16117..17490)
                     /locus_tag="VHF88_04195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX2161004.1"
                     /translation="MPTDLVTMDKIVALCKRRGFIFQSSEIYGGLASTYDYGHYGVLL
                     KSNVKAEWWRAVLQERDDMVALDAAILMHPRTWEASGHLEGFTDPLVQCLGDCKKRWR
                     EDHLREALEADGKDPSGELRCPQCGGDLSPPRNFNLMFETHMGPVQDEGSRIFLRPET
                     AQGIFVNFKNVLQFARKKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEIEFFVPPA
                     EAPQWHQRWMDERMRWYTDLGIRPDHLQLRKHEGDELSHYSSATSDIEYLFPMGWSEL
                     EGIANRGDFDLSQHAQFSREKLDYVDPGTGERYVPHVIEPSAGADRGTLAFMVDAYDE
                     EQVEGRERVVLRLHPRLAPVKAAVLPLLNKDGQPEKARAIYEELRKRLPAEFDSGGSI
                     GKRYRRQDEIGTPWGVTVDHQTMDDDTVTLRDRDSLDQTRIPAAELADELERRLKADW
                     SSPKLSA"
     gene            17561..17887
                     /locus_tag="VHF88_04200"
     CDS             17561..17887
                     /locus_tag="VHF88_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX2161005.1"
                     /translation="MPIYEYRCEKGHTFEVMQSMSAEPLNECEVCGAPVQKVLHAPAV
                     HFKGSGFYTTDYGKKKAAGAGADSKKADSNGSDSGSSSSSDSGPSSSDKGKKPDSKTS
                     SSAKSD"
     gene            complement(17889..18983)
                     /locus_tag="VHF88_04205"
     CDS             complement(17889..18983)
                     /locus_tag="VHF88_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2161006.1"
                     /translation="MNDSGHGDRPDYKVYRSRRSPLDKLRRSEDLEGFRDRSRPQEPR
                     RREPRAPRTHRTRKPITPRRVLRWVLTAIGAWLALSFVLFMVSAQLEGGVSDDAKDAL
                     SGNGNFLTGATVLVLGSDERKGDSIDATDSGPGRADSIMLVHASLGRVAKLSIPRDTE
                     ADIPGHGRTKINAAYAIGGPALMIETVEGYMGNGLEINHLIEVDFEDFPKFIDTVGGI
                     TVNNKSRICAPGFDNFWRGFNLPAGEQKLTGRQALGYARVRQNPCAPAENDLARAERQ
                     QEVLAGIRGALVSPATFFRLPWVSWRAPQTIRTDLRGPGLLALAADVATGGSGETRVL
                     EPSCLGCGVDASLQVPQDERAAAVEELLGR"
     gene            19007..19966
                     /locus_tag="VHF88_04210"
     CDS             19007..19966
                     /locus_tag="VHF88_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2161007.1"
                     /translation="MPPRRVIGIDAGGTKLLGGVVDEQLVVHHRVHRTWRGSDRAETL
                     EIFVAAVEEIRAAAPDVEAVGFGIPALVDHERGISVWSNHLPIDDVPFRALMSERLGL
                     PVVVDNDTNAAILAEHRHGAARGFQHAVMLALGTGIGGGLVIDGQVHRGTRGFAAELG
                     HITIDIHGEDCPGGCPGRGCFESLVSGRAIAVAARRLGEQEPGSTFGKLLAEGREIRG
                     GLVTELAHDGDEQARAALASVGARLGIGIAGLINALDPGVVVIGGGAVAAGDLLLDPA
                     RELVAQRSLPPGAEPPPILPARFGSESGMLGAALLALDGRSAT"
     gene            19963..20766
                     /gene="rsmI"
                     /locus_tag="VHF88_04215"
     CDS             19963..20766
                     /gene="rsmI"
                     /locus_tag="VHF88_04215"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX2161008.1"
                     /translation="MSLTVCPTPIGNLEDVTLRVLSALREADVVACEDTRRTRTLLDR
                     YGVNAKLVSYHEHNEEQRAAELVERMRAGANVALVSDAGMPLVSDPGYVLVRACVAAG
                     VAVEVLPGPSAPLAALVASALPADRWRFAGFLPRKAGELRKLLSEPGGTLIAFESPKR
                     VPATLALLAEVDPERQVAVCRELTKAHEEVVRGSAGELAARYADAPPRGEVVLVFGPA
                     TEAGDDGPPAGLDQVRRLVEAGAKPRVAAQVVAELTDGSANRLYSALTE"
     gene            20927..21658
                     /locus_tag="VHF88_04220"
     CDS             20927..21658
                     /locus_tag="VHF88_04220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2161009.1"
                     /translation="MVLAPASALAENWTWPVRGHVLAAYVNAGGPYAPGQHRGIDVSA
                     STGAPVVAAAGGKVRFAGLVGSSGLTVSVRTADGRFDTSYLHLATATVRAGQTVAPGQ
                     RIGTVGTSGRGSTAAPHLHFGVRYAGTGRYRDPLDFLPPLRGVRDTPRGAPVTVRGPL
                     RVGPAPQPVRPHPVPIGAAPLPHPIRPHRFPVADRRPIAAPASRTGWAIACAALICAA
                     ALLGTRSSIGARAVLRHHADLLRQR"
     gene            21615..23144
                     /gene="metG"
                     /locus_tag="VHF88_04225"
     CDS             21615..23144
                     /gene="metG"
                     /locus_tag="VHF88_04225"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX2161010.1"
                     /translation="MPYYVTTPIYYVNGEPHLGHVYTTVAADVLARHMRQRGDDVFFL
                     TGTDEHGEPVAQAAEREGIAPRELAERNAERFKEVARRVGATNDFFIRTSDPEHEAVV
                     AEIATRLEENGHVYQGVYEGWYCPRCADFKTDNEVLDGNKCPIHLIELDREQEQNWFF
                     RLSAFQAPLEALYAERPDWVLPRNRHNEALSFIQGGLNDLSLSRHGLSWGVPVPWDPE
                     QTIYVWIDALLNYYSALRYARPGEDLTERFWPPTVHLIAKDILKFHAIIWPALLMAAE
                     LEQPRRLFIHGYLLMDDHKMSKSLGNVLDPFQVMDVYGTDALRYYLLREVAFGQDGSI
                     SPEGFETRYTTELANEYGNLASRTLAMIGKYRDGVVPDADPAPELAMQFDGLAATVRE
                     RIDRVEVSAALEDVWERVRALNRYVADSEPWKLAKDESAAVELDVVLYTLAEGLRVVS
                     VLLAPWMPETTDRLLAALGHAGATLDSAELGARAGGARLGELGQLFPRVEPRVAEGAD
                     A"
     gene            23232..23981
                     /locus_tag="VHF88_04230"
     CDS             23232..23981
                     /locus_tag="VHF88_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2161011.1"
                     /translation="MIDTHCHLDSCKPGDAELVAHARTLGVTKIATVGMDPQTNPRAL
                     AAAAEFDGVSAIVGRHPNAAAGWRDDDIEEIERAAAQPGVVAVGETGLDYYRDRAPRD
                     DQRAAFVAQLELARRTGLPVAIHTRAAEDDTFAVLAEHADGLTVILHCFSAPARVEEA
                     VERGYLCSFAGNVTYKNAGDLQQAAREVPDELLLVETDAPYLTPVPLRGEPNEPANVT
                     HTAEFVAGLRGVEYAELEATVERNAARVFGW"
     gene            23978..24829
                     /gene="rsmA"
                     /locus_tag="VHF88_04235"
     CDS             23978..24829
                     /gene="rsmA"
                     /locus_tag="VHF88_04235"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX2161012.1"
                     /translation="MSQGSIDRLQRFDVRPNRELGQNFLVDDNILRLIGEAAELEPGD
                     VALEVGGGLGVLSEHLAPLVAHLHVVEIDRGLEAPLRDALDPHANVTLHFGDAVALDL
                     ATLDPAPTKIVANLPYGVAATVLLRTVSELPDARLWVAMVQREVGERLAAAPGSKVYG
                     ATSVLAQLACDVRVLRRVPATVFHPRPNVESAIVVLRRRGPSPANHVVALVHAAFAHR
                     RKALPGSLALDPGAPAGLRDAARTALEDLGLPADARAERLSPPEFANLAEALGADLLQ
                     PLRARRA"
     gene            24867..>25637
                     /locus_tag="VHF88_04240"
     CDS             24867..>25637
                     /locus_tag="VHF88_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161013.1"
                     /translation="MTAAPAVVEWAYAKVNLVLRVGPPRPDGVHPLCSLFAALDLADE
                     VRVTPLDGGGDDLVVCPGVQGGNLAAAAAAAFRSAVPDAALPALRIEIDKRIPVAAGL
                     AGGSADAAAVLRAANAIAGSPLSPDELRGVAAGLGSDVPSQIEPAHAIVAGVGERVER
                     VDLPPMTLVLVPRDEGLATAAVYAELDRLRAAAPTPAVGGADDPARLSDWLNPAPLRD
                     LASGSLARLAAGVANDLEPAALALRPELAEPLAALSDAG"
BASE COUNT         3738 a         9011 c         9162 g         3726 t
ORIGIN      
        1 gtcggggaag ccgcggccct cgttgatctc ggtctgctcg gcgcgcgaga cgacgtcgcg
       61 cgaggcgagc tccatcttgt tcggcgcgta cttctccatg aagcgctcgc cctgggcgtt
      121 gtagaggtgg gcgccctcgc cgcgcgctcc ctcggtgatc aataggccaa tccccgcgag
      181 cgtggtgggg tggtactgga ccatctccat gtccatcagc gcggcgccgg cgcggtaggc
      241 ctgggcgatg ccgtcgccgg tgcagatcag agcgttcgtc gtcgggtcga agatctggcc
      301 gttgccgccg gtggcgagga tcacggtctt ggcggtgaac agctcgatcg agccgtcgcg
      361 gatgttgcgc gtcaccgcgc cggcgatccg gccgtcggcg tccttgacca gcgccgtggt
      421 gaaccactcc tcgaagcgct cgacctcatc gtgcttcatc agctgctcgt agaggacgtg
      481 caggatcgcc tgaccggtga tgtctgcgac gtagtaggta cgggcggccg aggcgccgcc
      541 gaaggcgcgc gtgccgagct tgccctgctc gttgcggtgg aaggtgacgc cgaggtgctc
      601 gaggtggagg atctcgtccg gcgcctcgcg gcacatgatc tcgatcgcgt cctggtcgcc
      661 gaggtagtcc gagcccttga ccgtgtcgaa ggcgtgcgac tcccacgagt cctcggggtt
      721 cagcgccgcg ttgatgccgc cctgggccgc attggagtgc gagcggactg ggtggacctt
      781 ggagatgatt cccaccgacg ctccgccctc ggcggccgcg agggccgcgc gctggccggc
      841 gaggccggct ccgattacga ggacgtcgtg ctgtggcatg ggtggggagg ggtgaagcgg
      901 cggcgatcct atccgaacga tccggctgcg ggactctgcc cgcccggctc agacgacgac
      961 ggacgagccc tgaagcgcgc gccggacgtc gtcggcggtg atcacgccgc gcagctgccc
     1021 gtcgccgtcg actgccatca gcgccccgag ccggacgagc ccctcgcggc cgagcagcgc
     1081 ctcgagcggc tcgtcggcgc tgacgcgcag cgagctctcg gcgtcggcgg agtcattcgc
     1141 catcaccgac gcgacggtcc ggctcggccg caccgcctcg ggcaggccgc cgatgctctg
     1201 ctcggtcacc agcccgacga gccggccgtc ggcctcgacg accgggaacc agggccagcc
     1261 gtagcgcagg aagtactcgt cgaggcacgg tcgaccgtca gcccggctgg gatggccacc
     1321 ggctcggcgt ccatgacgtc ggcgaccgag acgccctcga tgcggctcgt cagctcggtc
     1381 tgggcgatcg ccccgcgcgc cgcctggttg aggaagacgc cgatgatccc gagccagatg
     1441 ccgctgacga tcgcgcccgt gagcgcgaag aggatgccgg cgccgatcat caggtaggcg
     1501 aagccgcgac cgagcgtccc ggcgaagcgc gtggcgcggt tgcggctgcc ggtcgccttc
     1561 caggcgatcg cgcgcgcgat ccgcccgccg tcgagcggga agcccgggat caggttgaac
     1621 agcaggagcg cgaggttgac gaagccgaga tagccgagga cggcaagcgt ctcgtcgtcg
     1681 ccgctggcgt cgaagccgac cgcgtgcgtc gcctcgtgcg atccggcgat cgcggccgcg
     1741 gccagccagc acgctgccgc gatcagcagc gtcaccagcg ggccggcggc cgccacgcgg
     1801 aactcctcac cggcgctctt ggagtcgcgc tccatcttcg ccacgccgcc gaacatccaa
     1861 aggtcgatgc cgctgatccc gatgcccgag cggcgtgcct cgaaggcgtg cccgagctcg
     1921 tgcagcagca cggagaggaa gaacagcagc gccgagacga ctgccagcgc gaacgccgtg
     1981 ctgccctgcg gaaagcggtc ctcgtagtag cccgagagtg accagatgat caggaagagg
     2041 acgaagaacc agctcgggtc caccccgatg cgtatcccga agacgcgcgc gacttggatc
     2101 gatccgccgc cgaacactcg atacatggtg gcagtaacct gcgcccgccg atcagcgacg
     2161 gaccagcgaa gggacgaccc ggtggcgaag atcgaagtca agaacccgat cgtcgagctc
     2221 gacggcgacg agatgacccg gatcatctgg gcgttcatca aggagcagtt gatccttccc
     2281 tacctggacg tcgagctcga gtactacgac ctcggcatcg agagccgcga cttgacctcg
     2341 gaccagatca ccgtcgacgc cgcaaatgcg atcaagcgcc acggggtcgg cgtcaagtgc
     2401 gcgacgatca cccccgacga ggcgcgcgtc gaggagttcg gcctcaagga gatgtaccgc
     2461 tcgccgaacg gcacgatccg caacatcctc ggcggcgtca tcttccgcga gccgattgtg
     2521 atctcgaaca tcccgcggct cgtgccgggc tggacgaagc cgatcgtgat cggccgccac
     2581 gccttcggcg accagtaccg cgccaccgac ctggtcgttc cgggcgaggg gtcgctgacg
     2641 ctcacctaca cgccgcgcaa cggtgacgac ccgatcgagc tcgacgtcta cgacttcccg
     2701 ggcggtggcg tcgcgatggc gatgtacaac ctcgacgact cgatccgcga cttcgcgcgc
     2761 gcctcgatgc gctacggcct cgagcgcggc tacccggtct acctctcgac caagaacacg
     2821 atcatgaagc gttacgacgg ccgcttcaag gacctgttcc aggagattta cgagagtgag
     2881 ttcaaggccg acttcgacgc tgccgggatc acctacgagc accggctgat cgatgacatg
     2941 gtcgccgccg cgctgaagtg ggagggcggc tacgtctggg cgtgcaagaa ctacgacggc
     3001 gacgtccagt ccgacaccgt cgcgcagggc ttcggctcgc tcgggctgat gacgagcgtg
     3061 ctgatgacgc ccgacggcaa gaccgtcgag gccgaggccg cgcatgggac ggtcacccgt
     3121 cacttccgtc agcaccagca gggcaagccg acctcgacca acccgatcgc atcgatcttc
     3181 gcctggacgc gtgcgctcgc cgcgcgtggc cggatggacg agacgccgac ggtcagcgag
     3241 ttcgccgaga cgctcgagcg ggtctgcgtg gagacggtcg agaacggcca gatgaccaag
     3301 gacctcgccc tgctggtcgg gcccgaccag ccgttcctga cgacgcagga gtttctcgcc
     3361 gctatcgacg gccgcctcga gcaggcgatg acgagtcgtt aacggccaac agccaactac
     3421 cccaggagag agtcaacccg tgagcaaccc cgtcaaggta accgtcaccg gcgccgccgg
     3481 gcagatcggc tatgcgctgc tcttccggat cgcgagcggg cagctgctcg gcccggacgt
     3541 ccccgtcgag ctgcgtttgc tggagattcc gcaggcgatc aaggccgccg agggaacggc
     3601 gatggagctc gacgactgcg cctttccgct gctcgccgga gtcgagatca gcgacgatcc
     3661 gaaccgcgcc ttcgacggcg tcaacatcgc gctgctcgtc ggcgcgcgac cgcgctcgaa
     3721 gggcatggag cgcggtgacc tgctcgaggc caacggcggc atcttccgcc cccagggcca
     3781 ggcgatcaac gcgcatgctg ccgatgacgt gaaggtgctg gtcgtcggta acccggccaa
     3841 caccaatgcg ctgatcgcga tgtcgaacgc ccccgacgtc ccgcgcgagc ggttcaccgc
     3901 gatgatgcgg ctcgatcaca accgtgcgat ctcccaggtg gcgagcaagc tcggccggcc
     3961 ggtgtcggat ttcaccaaca tgaccgtctg gggcaaccac tccgcgacgc agtatccgga
     4021 cctcttccac gcgcaggtcg acggccagag cgtcgccgag gcggtcggtg atcaggcgtg
     4081 gatcgaggag cagttcatcc cgaccgtcgc caagcgcgga gcggcgatca tcgaggcgcg
     4141 cggcgcgtcg agcgcagcgt cggccgccaa cgcggcgatc gaccacgtct acgactgggt
     4201 caatggcacg ccggacggag actgggtctc gatggccgtg ccgtcggacg gcagctacgg
     4261 cgtcgccgag ggaatcgtct cgagctttcc ctgcacgacc gtcgacggac agtggtcgat
     4321 cgtccagggc ctggagatcg acgacttctc gcgccagcgg atcgacgcct cggtctccga
     4381 gctatcggag gagcgcgagg cggtcaaggg actcggactg atctaggtcg gggggctcac
     4441 gccccccggg ccccccggtg gcgcctggcc ggcttgccag ggccccgggg tggcgggatc
     4501 aggccgggta ccagcccttg tccttcttct tcaccttgcc gtcctgctcc aggcctggca
     4561 ggacccggta caggtagttc ggctggatct tcatcgcctt ggcgagctcg gggatggtga
     4621 ttcccggctg accggcgacg aggtcgagcg cctgctgagc gcgcttgccc gagccgcgcg
     4681 gacggccacg gcgaccgctg ccggtcgatc ccgagctgcg gcgacgacgg gcgggcctcg
     4741 acggcgcgct ggtcgcggtg cggctacggc caccgccgcc attgccgtcg acgccctgca
     4801 gggcggcggc ggcctcctcg agctgccgat actcctcgac cagcggcgcg agctcgcgac
     4861 ggcgagcctc gatgtccttc agcgtcttct tcaggaagtc ggtcactgtg gggggaacct
     4921 cctcgtagtt cgcctggccg ggagggggta ggggatcccg agcagaccgt tatctgaaca
     4981 gtgttacaga tggtgaattc gggactgctc tccctgcgcc agaaggacca tgttcttatc
     5041 tccatggcac ccgcagtgga cggttactcg accatcgcgc gcaggcggtc gagcgactgg
     5101 tcgagctcac cggccgtgac gcgtcgtacg accggcccgg caaacctgcc gagcggtccg
     5161 ccggggaggg cgaactcgtt cgtgtacgtg aagcgcgtgc cgccgcctgc gttgggcgtc
     5221 agctcgtagg tcactcccgc gtgcgagcgc atcggtccgc tgccctccca gacgaccttg
     5281 cgtgccggtt gggcctctat gaccttccag tgaacggtga agccgcgacc ggcgagctgc
     5341 agcctttgtg tcagctcgga cccgcgctcg agcggtcccg cgggcgcgtg ctcgagcgcg
     5401 gcgtgaatcg tgacccaccg ctcgaggcag ttcggatcca tgatcacgtc gtagagtcgc
     5461 tccgggctcg ccgcgatgtc gatcgaacgc tcgaccttca ccaggccgcc agctcctcga
     5521 acgactccca ctcggcgagc gcgcggtcga tcgcccgctc cagtggcagc ggcttgattc
     5581 cgtacagctg cggcgctgcc agcgcattgc gtggcagcag gtcgtgctcg aggctctcca
     5641 tcagcgggcg gactagctcg agcggctgcc cggtgatcgc cgcgaccacc gcgctggcgg
     5701 gtggagtgag tgacacgccg agcccgacgg gcgtccggcc gacgcccatc tgctcggcga
     5761 tccgctcgat catctcgccg tagctcacca tgtcgggacc ggcgatgtcg agtgagcggc
     5821 ctgcggtagc gggaaggctc gcggttcgcg cgaggaactc gatcgcgtcg cgctcggcga
     5881 tcggccgcgt gacgttgtct cgccaggctg gcaggggcag caggcgcaag cgctcgacga
     5941 gccgtacgag gatccgaaac gacgccgagc gggcgccgat caggatcgag gcccgcaacg
     6001 ccgtcgcgcc tggtgcagcg tcgagcagga tccgctcgac ctcgaggcgc gatgcgatgt
     6061 gcggcgacac cggcccgttc gccggcgcga ggccgccgag atagacgacg cgctcgactc
     6121 cggcagcgct cgcatcggcg gcgaagcgct cggcggcgcg gcggtcacgc gtcgcgaagt
     6181 cgtgctcgcc ctcggccacg gcctccatcg aatggacgag gtagtaggcg accgagcagc
     6241 cctcgagcgc tgctcggacg ccggtgccgt cgagcaggtc gccctgggcg atctccacgc
     6301 cctccaacgg ctgcacgcgg acggaatcgc gtgcgagcgc gcgcaccgca cggccctcgc
     6361 tgcgcagtcg ttggacgagc cgtccgccga cgtagcccgt cgctccggtg accagctcca
     6421 tgggggccat tgaagccggt cggcacgggg gtctgcgccg cgaccgaagc tcgccccccg
     6481 ccaaacatcg caaagagcgg ggcagatcct gaaatccctg cattttcggg gaaacgcaaa
     6541 aagccgggat ttgcgggttc gggccctacc cacatgtgaa cgtttcgtaa ccatgctcgc
     6601 tccgccccag gcaagcgacc ccctcaggag agacgaccgc tagatgaaga agaaggcgaa
     6661 gcgcgtgagc tgcgaggact gcttcttcca gaagaaccag ttgtgcgcgc tggagctcga
     6721 agagccgtgc acgaccttcc ggcccgccga gcggaacctc gcgccggaga agcagctggc
     6781 gttctccttc cgcaccgagc ggacgtggtc ggcgtatgcg tttcctcagc cggggtagcg
     6841 gccgcacgac gggagacgaa tgcccgttcc gcttgggaat cgctgctcgg gcatccgtct
     6901 cccgccggcg gagcggcgct ggcggttgat cggctgaggt ctgtcacggg tagggtgccg
     6961 ccatgcggct gacggtgctt gggaagtccc cggcgtggca ggacatcgac ggggcgtgct
     7021 cggggtatct gctgcaagag ggggacacgg cggtcctgct cgactgcggc aacggcgtct
     7081 tcgggaagct gcgcaagttc gtcgactacg tcgacgtcga cgcggtggtg atctcccatc
     7141 cgcatgcgga ccacttcatc gacctcgtcc cctactccta cgcgctgatt catgcgccgc
     7201 ggcagcagcc ggttccggtg ccgccgtggc cggggaccga gtcgcccgcg cggccgcggc
     7261 tgatcgtgcc gccgggcgcg cgcgagacgt tccgcaaggt cgtcggcgcc tggggcaacg
     7321 aggacctgat cgagcaggcg tttgagctcg aggagtacgc ggtcggcacg gtcgtcgagg
     7381 tcgggccgct gcgcttcgac ttcgcggtga tgccgcactt cgtcgacgcc cacgcagtgt
     7441 cggtcacgtc ttcgaacggc ggcggccggc tggcgttcag cgccgactcg cggccggcgc
     7501 aagagctcgt cgagttcgcg cgcggcgccg acgtcttcct ggtcgaggcg acgttgccgc
     7561 gtccagagcg gagcggggtc cgcggccatc tgaccgccga ggaggcgggc gagcacggac
     7621 gcgacgccgc agtcggacgt gtcgtcctga ctcacatctc cgacgagctg gatctggagc
     7681 aggcggtcga gcgcgcggcg gaggccttcg gcgggccggt cgaggtcgca cgcgagggcg
     7741 ccgcctacga gatctgacgc tcgccgcgct cggttacgct gcgcgcgtga gccctgagcg
     7801 tgacctgttc gccaacttcg agcggatgcg gcgtgagatc gacgagctgt tcggcgacgt
     7861 cttcgagcgc gcggggctgc gcgggcgcgg cttctcgccg tcggtcgacg tctactacgt
     7921 cgacgatccg ccacgcgcgg tcgtccgggc ggacctcgcg ggagtcgaca tcgccgacgt
     7981 ggcgctcgag gtccgcggtc gccagctgtt gatcgcaggc gagcgccgaa ccggtcaggc
     8041 cgagaaccgg ctctaccagc agatcgagat cgagcatggg cactttcgcc gcacggtcga
     8101 gctcggcgcc gacgtcgtgg ccgaccaggc acgcgcgagc tacgaggacg gcgtgctggt
     8161 cgtcgaggtg ccgctggcgc gaccggagac ggccgtccgc cggatcgcga tccgcgaggc
     8221 gtccgagtag cggtgacgcc gacgatcgag atcccgacgc ccgacggacg cacccaggag
     8281 attccggtcg gcgcgacgcg cctgcccgag gcgctgccgg tgctgccgct caaggacagc
     8341 gtgcccttcc ccgagatgct gaccccgctg gccgtcggtc agccgcggtc ggtccagttg
     8401 gtcaacgacg tgctcggcgg caaccggatg ctcgtgatgg tcgcctcgcg cgatggcgag
     8461 gtcgagaacc cgggcccgga gcagctctat tcggtcgggg tcgtcggcgt cgtcgcgcga
     8521 atgctcaagg tgcccgacgg cagcctgcgg atcctcgtcc agggagggca gcgcgtcgag
     8581 ctcggcgaca cggtccagcg cgacccgtat ctgatcgcgc ggatcgtcgc cgcccccgac
     8641 gtgctcgagc cgtcgccgga gctcgaggcg ctgtcgcgca acgtccagca gacgttcacc
     8701 cagatcgtcg agcaggtgcc gtacctgccg gaggagctgc agctcgcgat cgccaacgtc
     8761 gacgatccgg cggagctcgc gcacatgatc gccggctcgc tgcggatcaa gaccgaggag
     8821 cgccaggagc tgctcgagga gcgcgacgtc gccaagcgcc tgcggcggct gtccgagctg
     8881 ctcgcacgcg aggcggagct gatctcgatc ggctcgaaga tccagtcgca ggtcgagtcc
     8941 gagatcgaca agggccagcg cgagttcttc ttgcgtcagc agctcaaggc gattcaggag
     9001 gagctcggcg agaccgacga ggcggccgcc gaggccgccg agctgcgcga gcggatcgag
     9061 gcggccggtc ttcccgagca cgccgccaag caggccgagc gcgagctgtc gcgcttcgag
     9121 cggctgccgc cgcagtcggc cgagcacggg acgatccgca actacctcga gtggctcgcc
     9181 gacctgccct ggtcgaagtc gaccgaggac cacatcgacc tcgaggcggc gcgcgaggtc
     9241 ctcgatcgcg accactacga catcgagaag gtcaaggacc ggatcctgga gttcctcgcg
     9301 gtgcgcaagc tcaagcccga cgcgccgagc tcgatcctct gcttcgtcgg gccgccgggc
     9361 gtcggcaaga cgtcgctcgg ccgctcgatc gccgcggcta tcggccgcga gttcgagcgc
     9421 atctcggtcg gcggcgtgcg cgacgagtcg gagatccgcg gccaccgccg cacctacgtc
     9481 ggcgcgatgc cgggcacgat cgtccgcgcg ctgcgcgacg ccggttcgaa caacccggtg
     9541 ctgatgatcg acgagatcga caagatgggc gccgacttcc gcggggatcc ctcgagcgcg
     9601 atgctcgagg tgctcgatcc cgagcagaac ggcagcttcc gcgaccacta cctcgacctg
     9661 ccgttcgacc tctcgaacgt cctcttcatc acgaccgcga accaactcga gccgatcccc
     9721 ggcccgctgc gcgaccggat ggagacgatc cagctcgccg gctacaccga gcaggagaag
     9781 ctcgagatcg ccaagcgcta cctggtcccg cgccagatcg agcgcaccgg gctcatgaag
     9841 tcgaagatcg agttcaccga cccggcgctg cgcgagctga tcgacggcta cacgcgcgag
     9901 gctggcgtgc gcagcctcga gcgcgagatc ggctcggtct gccgcaaggt cgcgcgcgag
     9961 ttcgccgagg ggacccgtcg cacgaagcgc cgaatcgggc cgaaggcggt cggcgagctg
    10021 cttggccggc gccggttcca ctccgacgtc gggcggcgca ccgacgagcc gggcgtggcg
    10081 accgggctgg cgtggacgcc ggtcggcggc gacgtgctgt tcgtcgaggc gaccgccttc
    10141 cccggggagg gcaagcttca gatcaccggc cagctcggcg acgtgatgcg cgagtccgcg
    10201 agcgccgcgc tctcctacgt ccgccgcaac ctggcgcgca tagcccccga tgcgccagcg
    10261 gactggttcg ccaagaacga cctgcacgtc cacgtgcccg ccggcgcgat ccccaaggac
    10321 ggcccgagcg ccgggatcac gatggcgacg gcgctcgcct cgctgctctc tggccgaccg
    10381 gtccgctccg acacggcgat gaccggcgag atcacgctga ccgggcaggt gctgccgatc
    10441 ggcggtctga aggagaaggc gctcgccgct cagcgggccg agatcacgcg cgtgatcgcg
    10501 ccgcgccgca acgaaggcga cctcgaggag tttccgccca acctgttggg gggtatgcag
    10561 ttcgtgttcg cggacaccgt cgacgacgtc ctggctgcgg ccttggactg acggttatcc
    10621 tcggaccgca cgtcaagccg aacgcgagga gagccatggc agccaagaag aaagccgcga
    10681 agggcgcagc cggagctatc gctgccgggc aggcgctgcg cgacaacccc tacgtccagc
    10741 ggctggtcga ggacgacgac ctgcgcgacg agctgcgctc cgcgttcgac tcggctcgca
    10801 aggcatacga ccggatcgat agcaagggcc ccgcgaaggc gctcgagaac aagaaggtcc
    10861 agcgcgacct caaggaggcc gccgagtcgc tcaagagcgc tgccgactcg ttgcgcgagc
    10921 cgcgcaaggg caagcacggc ttccgtcgcc tgttgatgct cgcggtgatc ggcgccgtgc
    10981 tggcgctcgt cctcagcgag gacctgcgca agaaggcgct cgacgcgctg ttcggcgccg
    11041 aggaggagta cgagtacacc tcgaccacca cgccgacgcc cgcggctgtc ccgaacggcg
    11101 ctgagactgc cgcgaacgcc tcctaggaca gcgggtccta gctggccggc cagctagact
    11161 ggtcggccaa tggaggcttc ggtagcagca ccacgcaagc tcgagggcga gaaggcgcag
    11221 cgcatcgtcg aggcgatgcg tgcgagcgtc ggcaagcgcg gcgccgccgg ttcgacgttc
    11281 gacgtcgtcg cgcgcgaggc gggcgtctcg cgcggcctgt tgcactacta cttcggctcc
    11341 aaggagcgcc tgatggtcga ggtcgtgcgc agcgactgcg atcagcgcgt cgcggcgctg
    11401 gaggagaacc tcgcaactgc gacgagcgcc gacgagttcg tcgcgttcct cgtccagacg
    11461 ctcgaggcgt tcctctccga cgagcccagc caagccgtcc tctacgagat gctcagcgcc
    11521 tcgcgtcgca gcgaggagat ccgtgctgag ctggccgagc tctaccgccg ctggcgcggg
    11581 cagctggccg ccgcgctgcg cgacaagcag gaggagggtg tcgtctcgct cggctccgag
    11641 cccgagaccg ttgccgcgct gctgttcgcg ctcggcgacg gcatggggct ccaggtgatc
    11701 tcggacccgg agtgggacag cgctccggcc ttcgagctcg gcttccagac cgcgcgccgc
    11761 ctgctgggcg cctaaccctc tgtgagccac gtcacagtcg ccgctaggcg gccgtgatcg
    11821 aatcccctta gactttggct ggctggccaa ctaaggccac cacgcgggaa aggggcggtt
    11881 atgcagcggt tcatggacag gctcagctca ttcctcgagc ggaggcgacg cttgttgctc
    11941 ggcgcgtggg tcgcgctgct gctcgtctcg ctgccgttcg cgatgaagca gaccgagcac
    12001 ctgaccagcg ggggattcac gatccccggc tctggctcag aggccgtcga ccacgcgctg
    12061 gcggacttcg acgacgccca gcgccagacg ctctcggtgg tcgtggcgcg ccgcccgggg
    12121 ggcgacgcgg ccgacgtgcg ccgcgagctc gcacgtgtag acacgatcgc cgacggcatg
    12181 gcgcacgccg agctgacgcc acgcgcgttg actgccgcga gccgcgacgc cgaccgctcg
    12241 ccgattgccg tcgcgccctt gaagaccgtg ggcgacatga acgacgccgc cgacctcgcc
    12301 gtcgacctgc gcaaggagat cggcatcggc gagggcccgc gcaacggcgt cgagacctac
    12361 ctcgtcggcg agcaggcgct ctgggccggc atgcaggacc tcagcaagaa ggacctccag
    12421 cgggcggagg cgaccggctt cccgatcgtc ctgctgatcc tgctcgccgt cttcggctcg
    12481 ctcgtcgccg cgatgctgcc gctggtgctc ggtttcgtca gcgtgatggt caccggggcg
    12541 gggatcttct tcgtctcgca ggcgaccgac atgtcggtgt tcgtgaccaa cgtcgcgtcg
    12601 atgatcggca tcggcgtggc ggtcgactac tcactgttca tcctctcgcg ctaccgcgag
    12661 gagattcgcg ccggcgccga cccggtcgac gcgcgacgca tcgcgatgcg gacgtccggc
    12721 gtggccgtcg ccttctcggg catcaccgtg atcgtgtcgc tcgccgggct gctgctcgtc
    12781 gactcgacga cgctgcgctc gatggcctac ggcgcgatca tggtcgtcgc ggtctcgatc
    12841 gttgcggcga tgacgttcct accggcgctg atggccgcga ccggcgagcg gggctatcgg
    12901 ccgggacgca cggccggcgt ggtcgcggcg atcgtcagcg ggctgcggcg gctgacgcgc
    12961 cgtccgccgc ggccggaggg cgcgccgggc ttctggcaga cctggaccga tcgcgtaacg
    13021 cgccgcccgc tcgtgaccgc gcttgccgcc agtgcgttga tgctgacgct cgcgatcccg
    13081 gcactgtcgc tgtcgaccgg tgacggggcc ctgcgccagt tccccgaggg caacgagacc
    13141 cgcgtcggcg ccgagctcgc cgccaagaag ctcggcccgg gcgccgcgtc gcccgtcgag
    13201 gtgctcgcga ccttcgccaa gggcaaggcg accgatcgct ccaaccagca ggctctgacg
    13261 agcttcgtcg gcgccgccaa gcaggacccc caggtcgcgg cgattcgacc gccggtgccc
    13321 tcggaggacg gcggggcggc gatgatcgcg ctgacgccac gcagcgatgc cgagagccag
    13381 cgggcgcgcg atctggtcga ccggctgcgc gacggcgaga gcggcgcggg agcgctgatg
    13441 aacgtggcgc gcgtcgacgt cggcggcgcc accgcggccg tcgaggactt ccgcgcccag
    13501 gtcaccggct ctctctggaa gatcgccctg ttcgtgctgg cgttcagcta cgtcgtgctg
    13561 ttcttcctgc tgcgctccgt gctgctgccg ctcaaggcgg tcctgatgaa cctgctgtcg
    13621 gtcggcgccg cctacggcgt cctcgtggcc gtcttccagt ggggctggat cgacggcttc
    13681 ctcggcttcc aatcgctcgg ctacatcaac acgatgacgc cgccgctgct gttggcgatc
    13741 gtcttcgggc tgtcgatgga ctacgaggtc ttcctgctga gcaggatcaa ggagcgctac
    13801 gccgccaccg gggactcgaa agtcgcggtc gcgcaggggc tcgcgcgcag cgcggccacg
    13861 atctcgagcg cggcgctgat catggtcgcc gtgttcgcgg tcttcgccgg cgtcggcgtg
    13921 ccgtcggtca aggagatcgg ggtcggcctg gcggtcgcgg tggcgctcga cgcgacgatc
    13981 gtgcggctgg tgttggtccc cgcgacgatg gagatcatgg gcgactggaa ctggtggctg
    14041 ccgcgcccgc tcgatcgcgt gatgccagac gccgacttcg aggcgtcgga gtctcccgcc
    14101 cctgcgcttg tcgggtcgcg ctccggctag ccgtcgccag gaactcgtcg aggtggcgcc
    14161 aggtcgtcgc ctcggcgccg cggccggtga gcgcgccgag atgactaccg gtgacgagcc
    14221 ggtaggtcac cgatgcggcg ttgggcagca ggtcgacgac cgcctcgacg gagcctgccg
    14281 gggcgagcac gtcgtcctca ctcgcgatga cgagcaccgg ctgacgcacg ttggccagct
    14341 cgatctggtg gtcgccgagg tcgacgaccc cgtcagcgag gtcgttgacg cggaagaagc
    14401 ggtggtagag ctgaccgaag gtgcgacccg ggtaggcgag catgttcgcc atgtagctgt
    14461 cgatcgcctg ggagtgggcg atcgagtcgc gatccgggag gttgcgcagg acccagagcg
    14521 gcttggtcag gtagcgatcg atcgaggtca gctggaagac gcgactgacc agcggggccg
    14581 gcgccccgcc gagcgcccgg tagagcgcgc tctccagcgc cccgccggtc agtcgcccga
    14641 tctggcgcag cggggcggcg aggcggacgc gcgtgaagtc gaacggactg gcgatcaccg
    14701 ccgccgaggc gaccgggagc tcctggtcgc ttgcgacggt cagcagcgtc aagatgccgc
    14761 cgaggctcca gccgaccagc tgcaccggct ggccgccgct gtcctcgtct acgacctcga
    14821 tcgcggtcgg cagcacgtcc tcgacccagt gctcgagccc gagcgcgcgg tcgctgaagg
    14881 agatcggccc gtaatcgacg acgtaggtcg gatagccgag cgagatcagg tgctcggcca
    14941 gactgcagcc gcgctgcagg tcgaagcagc tcgccggggc cgcgagcggc gggatcagca
    15001 ggaccggcgc gcggtcggcc cggctgcggc gtcgcgagcg gaagcggcgc acggtgcact
    15061 gcggtccctc gtggacgatc gaggagggcg tcggacgcag gtccgcgacg cccggtccga
    15121 cgtagaggtc ccacgccgtg gcggcggagt cgacgagctg ctcgggatcg gggaggagtc
    15181 gcatgccggg ccggtcggcc gctcggcgcc tcaggcggcg tgagcgagcg agccggggaa
    15241 gtctaggagc agctcggcga gccggtccgc cgcctcgatc tgcgggcagt ggccgcagtc
    15301 ctcgatcagc gtcagctctg cgtccgggac gctttcgagg actcgttcgg cgccgctctt
    15361 gaagaccatc gcgtcgtggt cgccccagac cagccggact gggcagccga tccgcccgag
    15421 ctcgaagggg tcgcgcagct ccggcagcat gcggcgggcg gcgcccagga tttgcgcagt
    15481 ggtgcggcgg tcgcggaagt gcgaggtgaa ggcacggccg atcagcggct cgatcgcctc
    15541 agggtggtgg aagaccagcc gcttgtagat ctcgaggacg acccgctgga gcacctgggg
    15601 cggtaccggc agcggcgatg ccagcagcgc cctcagaacc ggctcgcgct cgatcagggt
    15661 gagccagcgc gccatgtcga ggccggcggg cgcgaccggc acgatgcccg cgagacgcag
    15721 gccgcggcgc tcggcgagca gcagcgacag gcaaccgccg agcgagttgc cgcagacgat
    15781 cgccccgcct ttgggtgcat ggtgctggac ggccgccttc gcgaaggccg tcagctgggc
    15841 gagcaccggc tcgtcgggac gcagcgggtc cgcggtgcca aagcccggca ggtcgaaggc
    15901 gacggcgcga cggtcgccgc ggccgaggcg atcgagcagc gtgcgccagg tatcggcgct
    15961 gtcggcgaac ccgtgtaaca ggatcagcgg cggaccggag ccctcgagct cgagggctcg
    16021 cgtctcgaat ccggctagct cgagccggtg ctcgaacaac ggctgcatcc tgtcgcgagg
    16081 ataggccgcc cggcggccga cgtgcgccgg ttggcgctac gcggagagct tcgggctgga
    16141 ccagtcggcc ttcagccgcc gctcgagctc gtccgccagc tcggcggccg ggatccgggt
    16201 ctggtcgagc gagtcgcggt cgcgcagcgt cacggtgtcg tcgtccatcg tctggtggtc
    16261 gacggtcacc ccccacggcg tgccgatctc gtcctggcgg cggtagcgct tgccgatcga
    16321 gccgccgctg tcgaactcgg ccggcagccg cttgcgcagc tcctcgtaga ttgcgcgcgc
    16381 cttctccggc tgaccgtcct tgttgagcag cggcagcacg gccgccttga ccggcgcgag
    16441 gcggggatgg aggcgcagca cgacccgctc gcggccctcg acctgctcct cgtcgtaggc
    16501 gtcgaccatg aacgcgagcg tgccgcgatc ggcgcccgcc gacggctcga tcacgtgtgg
    16561 cacgtagcgc tcgccggtcc ccggatcgac gtagtcgagc ttctcgcgcg agaactgcgc
    16621 gtgctggctg aggtcgaagt cgccgcggtt ggcgatcccc tcgagctcgg accagcccat
    16681 cgggaacagg tactcgatgt cgctggtcgc gctcgagtag tgcgacagct cgtcgccctc
    16741 gtgcttgcgc agctggaggt ggtcggggcg gatgccgagg tcggtgtacc agcgcatccg
    16801 ctcgtccatc cagcgctggt gccactgcgg cgcctccgcc ggcggcacga agaactcgat
    16861 ctccatctgc tcgaactcgc gcgtgcggaa gatgaagttg ccaggcgtga tctcgttgcg
    16921 gaacgacttg ccgacctggg cgatgccgaa tggcggcttc ttgcgcgcga actgcaggac
    16981 gttcttgaag ttgacgaaga tgccctgcgc ggtctcggga cgcaggaaga tccgcgaccc
    17041 ctcgtcctgg actggcccca tgtgggtctc gaacatcagg ttgaagttgc gcggtggaga
    17101 caagtcgccg ccgcactgcg ggcagcgcag ctcgccgctc ggatccttgc cgtcggcctc
    17161 gagcgcctcg cgcagatggt cctcgcgcca gcgcttcttg cagtcgccga ggcactggac
    17221 gagcgggtcg gtgaacccct cgaggtggcc cgacgcctcc cacgtgcgcg ggtgcatcag
    17281 gatcgccgcg tcgagcgcca ccatgtcgtc gcgctcctgc agcaccgcgc gccaccactc
    17341 ggccttgacg ttggacttca gcagcacgcc gtagtggccg tagtcgtagg tcgaggccag
    17401 gccaccgtag atctccgacg actggaagat gaagccgcgc cgcttgcaga gggcgacgat
    17461 cttgtccatg gtgaccaggt cggtcggcat cggccgaacc ctagaagaag caagcgtgct
    17521 cggcggtcag cgtcggcccg ctcgtatact ggccggcccc atgccgatct acgagtaccg
    17581 ctgcgagaag ggccacacct tcgaggtgat gcagagcatg agcgccgagc cgctcaacga
    17641 gtgcgaggtc tgcggggcgc ccgtccagaa ggtcttgcat gcgcccgcgg tccacttcaa
    17701 gggctccggc ttctacacga ccgactacgg caagaagaag gccgccggcg caggtgccga
    17761 ctccaagaag gctgactcca acgggtcgga ctcaggatcg tcgagcagct ccgactccgg
    17821 gccctcgagc tccgacaagg gcaagaagcc ggactcgaag acgagctctt ccgcgaagtc
    17881 cgactgagct aacggccgag tagctcctcg accgcggcgg cgcgctcgtc ctgcggcacc
    17941 tggaggctgg cgtcgacgcc gcagccgaga caggacggct cgagcacgcg cgtctcgccc
    18001 gagccgccgg tagcgacgtc ggcggcgagc gcgagcaggc cgggcccgcg caggtccgtg
    18061 cggatcgtct gcggcgcgcg ccagctgacc cacggcaggc ggaagaaggt cgccggcgag
    18121 accagcgcgc cgcggatgcc ggccagcacc tcctgctggc gctcggcgcg ggcgaggtcg
    18181 ttctcggcgg gcgcgcatgg gttctggcgc acgcgcgcgt agccgagcgc ctggcgcccg
    18241 gtcagcttct gctcgcccgc gggcaggttg aagccgcgcc agaagttgtc gaagcccggc
    18301 gcgcagatcc ggctcttgtt gttgacggtg attccgccga ccgtgtcgat gaacttgggg
    18361 aagtcctcga agtcgacctc gatcagatgg ttgatctcga gcccgttgcc catgtagccc
    18421 tcgaccgtct cgatcatcag cgccggtccg ccgatcgcgt aggcggcgtt gatcttggtc
    18481 cggccgtgac cggggatgtc ggcctccgtg tcgcgcggga tcgacaactt ggcgacgcgg
    18541 ccgagtgagg cgtggacgag catgatcgag tcggcgcggc ccgggccgct gtcggtcgcg
    18601 tcgatcgagt cgcccttgcg ctcgtcggag ccgagcacca ggaccgtcgc gccggtgagg
    18661 aagttgccgt tgccggagag ggcgtccttg gcgtcgtcgg agacaccgcc ctcgagctga
    18721 gcgctgacca tgaagagcac gaacgacagc gcgagccagg cgccgatcgc cgtcagcacc
    18781 cacctgagga cgcgccgcgg ggtgatcggc ttgcgcgtgc ggtgcgtccg cggggcgcgt
    18841 ggctcgcgtc gccgcggctc ctgggggcgc gagcggtcgc ggaacccctc caggtcctcg
    18901 gagcggcgga gcttgtcgag cgggctcctg cgcgaccggt agaccttgta gtccggccgg
    18961 tcgccgtggc cggagtcgtt caccaccctc tgtatatcgt ccggccatgc cgccacgacg
    19021 cgtcatcggg atcgacgcag ggggcaccaa gctgctcgga ggggtcgtcg acgagcagct
    19081 cgtcgttcac caccgggtgc atcggacgtg gcgcggctcg gaccgtgcgg agacgctgga
    19141 gatcttcgtc gcggccgtcg aggagatccg cgccgccgcg ccggacgtcg aggcggtcgg
    19201 cttcgggatc ccggcgctgg tcgaccacga gcgtgggatc tcggtctggt ccaaccatct
    19261 gccgatcgac gacgtgccgt tccgggccct gatgagcgag cggctcgggc tcccggtggt
    19321 ggtcgacaac gacaccaacg cggcgatcct cgccgagcac cgccacggcg cggcacgcgg
    19381 gttccagcac gcggtgatgc tggcgctcgg caccggcatc ggcggcgggc tggtgatcga
    19441 cggccaggtg caccgtggca cgcgcggctt cgccgccgag ctcggccaca tcacgatcga
    19501 catccacggc gaggactgcc ccggcggctg ccccggccgc ggctgcttcg agtcgctcgt
    19561 ctccggccgg gcgatcgcgg tagcggcgcg gcggctcggc gagcaagagc ccgggtcgac
    19621 cttcggcaaa ctgctcgcgg agggtcgcga gatccgcggc ggcctggtca ccgagctcgc
    19681 ccacgacggc gacgagcagg cgcgcgccgc cctcgcgagc gttggagcgc ggttggggat
    19741 cgggatcgcc gggctgatca acgcgctcga tcccggcgtc gtcgtgatcg gcggcggcgc
    19801 cgtcgccgcc ggcgacctcc tgctcgaccc cgcccgcgag ctggtcgccc agcgctccct
    19861 gccgccggga gccgagccgc ccccgatcct ccccgcccgc tttggcagcg agtcaggaat
    19921 gctcggcgca gccctcctcg ccctcgacgg acggagcgcg acgtgagtct gaccgtctgc
    19981 ccaaccccga tcggcaacct cgaggacgtc accctgcgcg tcctctcagc gctgcgcgag
    20041 gccgacgtcg tcgcctgcga ggacacccgc cgcacccgca cgctgctcga ccgctacggc
    20101 gtcaacgcga agctggtcag ctaccacgag cacaacgagg agcagcgggc ggccgagctg
    20161 gtcgagcgga tgcgggcggg cgcgaacgtg gcgctcgtct ccgatgccgg catgccgctg
    20221 gtctccgacc ccggctacgt gctggtgcgc gcgtgcgtcg cggcgggcgt tgcggtcgag
    20281 gtgctgccgg ggccgtcggc gccgctcgcc gcgctggtcg cctcggcact gcccgccgac
    20341 cgttggcgct tcgccggctt cctgccgcgc aaggccggcg agctgcgcaa gctgctctcc
    20401 gaacccggcg ggacgctgat cgccttcgag tcgccgaagc gcgtgccggc gacgctggcg
    20461 ctgctcgccg aggtcgaccc ggagcgccag gtcgcggtct gccgcgagct gaccaaggcg
    20521 cacgaggagg tcgtccgcgg cagcgccggc gagcttgcgg cgcgctacgc cgacgctccg
    20581 ccgcgcggcg aggtcgtgct cgtcttcggc ccggcgaccg aggccggcga cgacgggccg
    20641 cccgcgggcc tcgaccaggt tcgccgcctc gtcgaggcgg gcgccaagcc gcgggtcgcc
    20701 gcgcaggtgg tggctgagct gaccgacggc agcgccaacc gcctctattc ggcgctcacg
    20761 gagtaacgga cgccgccgcc actgcacggg ttcgcgcagg atcgagacag ctcggtgagt
    20821 gaggtggcgc tgtagggcgg cccgcgccta cggtcggccg gtgcgcaact tgccgatacc
    20881 cgcgaccggc tcgagcgcag ccatcatcgc tgcgacgatc gctctagtgg ttctggctcc
    20941 ggcctccgcg ttggcggaga actggacgtg gccggttcgt ggtcacgtgc tcgccgcgta
    21001 tgtgaacgct ggcggcccgt acgcgccggg gcagcaccgc ggcatcgacg tctcagcatc
    21061 gacgggggcg cccgtcgtgg ccgccgctgg cggcaaagtc cggttcgccg gcctcgtcgg
    21121 ctcatccggc ctcacggtct cggtccggac ggccgacgga cgcttcgaca cctcctacct
    21181 ccacctcgcg acggccaccg ttcgcgcggg acagaccgtt gcgcccggcc agcggatcgg
    21241 caccgtcggc accagcggcc gcggctcgac cgccgcgccg cacctccact tcggcgtccg
    21301 ctacgccggc acgggccgct accgcgaccc gctcgacttc ctgccgccgc tgcgcggcgt
    21361 gcgcgacaca ccgcgcggcg ctcccgtcac cgtgcgcggc ccgctacgcg tcggccccgc
    21421 tccccagccc gtccgcccgc atccggtccc gatcggcgcc gcgccgttgc cgcacccgat
    21481 ccgcccgcac cgcttccccg tggccgaccg ccgcccgatc gcggcgccgg cctcgcgcac
    21541 gggttgggcg atcgcctgtg ctgcgctgat ctgcgcggcg gcgctgctgg gcacgcgcag
    21601 tagcatcggc gcccgtgccg tactacgtca ccacgccgat ctactacgtc aacggtgagc
    21661 cccacctcgg tcacgtctac acgacggtcg ccgccgacgt gctggcgcgg cacatgcgcc
    21721 aacgcggcga cgacgtcttc ttcctgaccg ggaccgacga gcacggcgag ccggtcgcgc
    21781 aggcagcgga gcgcgagggg atcgcgccgc gcgagctggc cgagcgcaac gccgagcggt
    21841 tcaaggaggt ggcccgccgc gtcggcgcca ccaacgactt cttcatccgg acgagcgacc
    21901 ccgagcacga ggcggtcgtc gccgagatcg cgacccgcct cgaggagaac ggccacgtct
    21961 accagggcgt ctacgagggc tggtactgcc cgcgctgcgc cgacttcaag accgacaacg
    22021 aggtcctcga cggcaacaag tgcccgatcc acctgatcga gctcgaccgc gagcaggagc
    22081 agaactggtt cttccggctg tcggcgttcc aggcgccgct cgaggcgctg tacgcggagc
    22141 ggcccgactg ggtgctgccg cgcaaccgcc acaacgaggc gctgtcgttc atccagggcg
    22201 ggctcaacga cctgtcgctc agccgccacg gcctcagctg gggcgtgccg gtcccgtggg
    22261 accccgagca gacgatctac gtctggatcg acgcgctgct caactactac tcggcgctgc
    22321 gctacgcccg tcccggcgag gacctgaccg agcgcttctg gccaccgacc gtccacctga
    22381 tcgccaagga catcctcaag ttccacgcga tcatctggcc ggcgctgctg atggccgccg
    22441 agctcgagca gccgcgccgg ctgttcatcc acggctacct gctaatggac gaccacaaga
    22501 tgtcgaagtc gctcggcaac gtgctcgacc cgttccaggt gatggacgtc tacggcaccg
    22561 acgcgctgcg ctactacctg ctgcgcgagg tcgcctttgg ccaggacggc tcgatctcgc
    22621 ccgagggctt cgagacgcgc tacacgaccg agctcgccaa cgagtacggc aacctcgcca
    22681 gccgcacgct ggcgatgatc ggcaagtacc gcgacggcgt ggtccccgac gcggacccgg
    22741 cgcccgagct ggcgatgcag ttcgacggcc tcgcggcaac cgtgcgcgag cggatcgacc
    22801 gcgtcgaggt gagcgccgcg ctcgaggacg tctgggagcg ggtgcgggcg ctgaaccgtt
    22861 acgtcgccga cagcgagccg tggaagctcg ccaaggacga gtcggccgcc gtcgagctcg
    22921 acgtcgtcct ctacacgctc gccgaggggt tgcgcgtcgt gtcggtgctg ctcgcgccgt
    22981 ggatgccgga gacgaccgac cggctcctgg ccgcactcgg acacgcggga gcgacgctcg
    23041 acagcgcgga actcggcgcc cgggctggcg gcgcccgcct cggtgagctg ggccagctgt
    23101 tcccgcgcgt cgagcccagg gtggccgagg gcgcggacgc gtgatgcccg ctgctaacgc
    23161 ctcgatgtcg cattcgtcgc ccctggcgat gcgaatccga catcgggcgc ccgcgtgagg
    23221 ttgccgcggg cgtgatcgac acccactgcc acctcgactc ctgcaagccc ggcgacgccg
    23281 agctggtcgc ccacgcccgc acgctcggcg tcacgaagat cgcgaccgtc gggatggacc
    23341 cccagaccaa cccgcgcgcg ctcgcggcgg ccgccgagtt cgacggcgtg tcggcgatcg
    23401 tcggccgcca ccccaatgcc gccgccggct ggcgcgacga cgacatcgag gagatcgagc
    23461 gcgccgccgc ccagccgggc gtcgtcgcgg tcggcgagac tgggctcgac tactaccgcg
    23521 accgcgcgcc gcgcgacgac cagcgcgcgg ccttcgtcgc gcagctcgag ctggcgcgcc
    23581 gtaccgggct gccggtcgcc atccacacgc gcgccgccga ggacgacacg ttcgccgtgc
    23641 tcgccgagca cgccgacggc ctgacggtga tcctccactg cttctcggcg cccgcgcgcg
    23701 tcgaggaggc ggtcgagcgc ggctacctct gctcgttcgc cggcaacgtc acctacaaga
    23761 acgccggcga tctccagcag gcggcgcgcg aggtgcccga cgagctgctg ctggtcgaga
    23821 ccgatgcgcc ctacctgacg cccgtcccgc tgcgcggcga gccgaacgag ccggccaacg
    23881 tcacccacac cgcggagttc gtcgccggcc tgcgcggcgt cgagtacgcc gagctggagg
    23941 cgacggtcga gcgcaacgcg gcgcgggtct tcggctggtg agccagggca gcatcgaccg
    24001 gctgcagcgc ttcgacgtgc ggccgaaccg cgagctcggc cagaacttcc tcgtcgacga
    24061 caacatcctg cggctgatcg gcgaggcggc agagctcgag ccgggcgacg tggcgctcga
    24121 ggttggggga ggcctcggcg tcctctccga gcacctggct ccgctcgtcg cgcacctgca
    24181 cgtcgtggag atcgaccgcg ggctcgaggc gccgctgcgc gacgcgctcg atccgcacgc
    24241 caacgtcacg ctgcacttcg gcgacgccgt cgcgcttgat ctcgcgacac tagatccggc
    24301 gccgacgaag atcgtcgcca acctgcccta cggcgtcgcc gcgacggtcc tactgcggac
    24361 ggtgtccgag ctgcccgacg cgcggctctg ggtcgcgatg gtccaacgcg aggtcggcga
    24421 gcggttggcc gccgcccccg gatcgaaggt ctacggcgcg acgtcggtgc tcgcccagct
    24481 ggcctgcgac gtgcgcgtgc tgcgacgcgt cccggcgacc gtcttccacc cgcggccgaa
    24541 cgtcgagtcg gcgatcgtcg tgctgcgccg gcgcggcccc tcgccggcga accacgtcgt
    24601 cgcgctcgtc cacgccgcct tcgcccatcg gcgcaaggcg ctgccgggtt cgctggcgct
    24661 cgatccgggc gccccagccg ggctgcgcga cgccgctcgc acggcgctcg aggacctcgg
    24721 gctgccggcg gacgcgcgcg cagagcgact gtccccgccc gagttcgcca accttgcgga
    24781 agcgctcggc gccgacctgc tgcagccgct gagggcgaga cgcgcatgag ccgaggggag
    24841 ccgccgcact tcgcgaaggg acgacagtga ccgccgcgcc cgcggtcgtc gagtgggcct
    24901 acgcgaaggt caacctcgtc ctgcgtgtcg ggccgccgcg gcccgacggg gtgcacccac
    24961 tctgctcgct gttcgcggcg ctcgacctcg cggacgaggt gcgggtgacg cccttggacg
    25021 gcggcggtga cgatctcgtc gtctgtcccg gcgtccaggg aggcaacctc gccgccgcgg
    25081 ccgcggcggc ctttcgatcg gccgtgccgg atgccgcgct gccggcgctg cggatcgaga
    25141 tcgacaagcg gatccccgtt gcggcgggcc tggccggcgg aagcgccgac gccgccgccg
    25201 tgctccgcgc ggcgaatgcg atcgccgggt cgccgctgtc gcccgacgag ctgcgcggcg
    25261 tcgccgccgg cctcggctcg gacgtgccga gccagatcga gccggcgcat gcgatcgtcg
    25321 caggcgtggg ggagcgggtc gaacgggtag acctaccgcc catgacgctc gtgctcgtcc
    25381 cgcgcgacga aggcctcgcg acggccgccg tgtacgcgga gctcgaccgc ctgcgcgccg
    25441 ctgcgccgac gcccgccgtg ggaggcgctg acgatcccgc ccgcctctcc gactggctca
    25501 acccagctcc cctgcgcgac cttgcgagcg ggtcgctggc gcggctcgcg gccggggtcg
    25561 cgaatgatct ggagccggcg gcgctcgcgc tgcggccgga gctggccgag ccgctcgcgg
    25621 cgctgagtga cgccggg
//
LOCUS       DATABV010000099         4843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1394874, whole genome shotgun sequence.
ACCESSION   DATABV010000099 DATABV010000000
VERSION     DATABV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394874"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4548
                     /locus_tag="VHF88_04245"
     CDS             <1..4548
                     /locus_tag="VHF88_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161014.1"
                     /translation="RDDGTGGRPTPRSQRPRLLAALLPLLALAALAQAPAHAAPKSIN
                     GFVGGGAGINSGGLFTQPRDVAVYTAGDSDPANDKILVVEGLGNNSRVQRLDRHGNFE
                     LIWGKDVVRAGAPGNLGTGFEICSEAVGGAESCKSAPTGAAEGEFDNPGGIAVDQSTG
                     HVYVHDRNNLRVQEFTLDGEFVRAWGWGVATGAAELEVCTANCRKGLAGGGDGQLGDT
                     GVTGAGIAVDPVNGDVFVADPANRRAQQFSATGAFVAKFGVAGGGVGEFGSSSPSRIA
                     VDSSHVVYLSDTNASDRVQRYDSVADVFLAPIECCAPTVGAPLAAGATVGIEVAADGD
                     GPGPDREALLVARDPASGPAVVQELDIPTPPTDPVTAVVDTHTYADEFISGIAHSDTD
                     GHVYVTVPGLFSPPNGPFAFPCPLSAAGTFVCSGLVALAESAGVVSAQLVDPPSRVGS
                     TSLAVTGSVNPAGGVARYRFEVSTDGSEWRDTGVGGYAAGTADVEAPGELSALQPATP
                     YKVRLRAEKHTSITTVQSASSNEVTTVTAIAPPDVSTRRAVYVTDTSAELRARIDPNG
                     IETTYRFEYGLAGGPLDRQVPLTDAGAGTGNDPQLFVEDLAGLEPATAYAFRIVATNA
                     AGESVGATVAFETRELDDVYAPPGRGYELVSPADKVAGVGTGVWYNGPAAIEEVGFGA
                     HRGERFAAKGTLGATLLDGPYAYANDWALAERTDSGWKSGPGASRRAYGSQHKTFMTM
                     SSATPDLSTMGWTSNGHTVKPFPEMEPWEETRVGNVLLLRRWAGGEWELFGPLAEGHV
                     TDNQPELSEAVAEDGTAVAATALRVRGLAGAGDPTSPAFDDLVEGSNVYVDETPGAFS
                     DVFPGDDGVRELVNVCTAGTVLPTRVQAGAFFKQGTAACPDALAGRDSRLVSPYGASL
                     GTDKEGVISADGSRVFFMAPDVRVPQSTAGCSGVGADTVCPTQLYVRQRSSDGSVVTR
                     WISRTQVTAANGAAADQDASLIGPAFFEGASADGDKVFFRTNSPLTADDPNGLGSAPA
                     GGVTTGDAHGESWDLYMYDMPDGQDADPAGGELVRISRGPGGQGDCNSPFGGAGSDRP
                     GALRFVSADGARAFFTCAAPLPGVADPGSGTITDPTGARDTAATSNLYVYDAGRSGAD
                     RWRFVALLPRTSPLGACATRSAELGSTLYGSGNDVNLSNVGSCVSGTADGAFVTFFTD
                     GALTIDDPDDGVGDVYGYSATRDQLHRLSKAQGGAGGAYPCRPQTPAPMCHGDGGIGP
                     ERMPVKRLGVASEPTNQTDRIAYFQSRSQLVAEDTDDAYDVYEWRNGDLTLLSTGQSP
                     TDGAFYVGNDATGRNVFMATRDRLSWEDSDAVLDVYSARAGGGFDRPPVHRPCDVLGD
                     GCQGTGGSAVGPPIGSDAPGGSNAKPQRRIALTLRTHLSRRARQLAARRGVVPVRVRV
                     SGPTLVRVAARAPVGGRLRRAGTARKRVHKAGTVTVRLRVRGQARRRLRSGRAVRLRL
                     VVRAKGAVPEKVTIVLKRGRR"
     gene            4581..>4843
                     /locus_tag="VHF88_04250"
     CDS             4581..>4843
                     /locus_tag="VHF88_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161015.1"
                     /translation="MAGTGKVGRRRVLVVALALMALLSAVTASQARAAFGDLFGLAPV
                     NGPGLPASPVFPATAGVDHAFWAGTCDVGSAPFGPIAGGVGSRP"
BASE COUNT          663 a         1686 c         1820 g          674 t
ORIGIN      
        1 cgcgacgacg gcaccggcgg caggccgacg ccgcgatcgc agcgtccgcg actgctcgcc
       61 gctctcctgc cgctgctggc gctcgccgcg ctggcgcagg cgccggccca tgcggcgccg
      121 aagtccatca acggcttcgt cgggggcggg gccggcatca acagcggcgg cctgttcacc
      181 cagccgcgtg acgtcgcggt ctacacggcg ggcgactccg acccggcgaa cgacaagatc
      241 ctcgtcgtcg agggcctcgg caacaacagc cgcgtgcagc gcctcgaccg gcacggcaac
      301 ttcgagctga tctggggcaa ggatgtcgtc cgtgccggag cgccgggcaa cctcgggacg
      361 ggcttcgaga tctgcagcga ggccgtcggc ggcgcggaga gctgcaagtc ggcgccgacg
      421 ggagccgcgg agggcgagtt cgacaacccc ggcggcatcg ccgtcgacca gtcgaccggc
      481 cacgtctacg tgcacgatcg aaacaacctg cgcgtccagg agttcaccct cgacggcgag
      541 ttcgtgcgcg cctggggctg gggggtggcg acgggcgccg ccgagctcga ggtctgcacc
      601 gcgaactgcc gcaaggggct cgcaggcggg ggcgacggtc agctgggcga caccggcgtc
      661 acgggcgccg gcatcgcggt cgacccggtg aacggcgacg tcttcgtggc cgatcccgcc
      721 aaccggcgcg cacagcagtt ctcggcgacc ggcgcgttcg tcgcaaagtt cggtgtggcg
      781 ggcgggggcg tgggcgagtt cgggtcgagc tcgccgtcgc gaatcgcggt cgactcgagc
      841 cacgtcgtct acctgagtga cacaaacgca tcggaccggg tccagcgcta cgactcagtt
      901 gcggacgtgt tcctcgcgcc gatcgagtgc tgcgctccga cggtcggcgc gccgcttgcc
      961 gccggcgcga ccgtcggcat cgaggtcgcg gcggatggcg acggccccgg tccggaccgc
     1021 gaggccctgc tggtcgctcg ggatcccgct tccgggcccg ccgtggtcca ggagctcgac
     1081 atcccgacac cgccaacgga cccggtcacc gcggtcgttg acacccatac ctacgccgac
     1141 gagttcatat cgggtatcgc gcacagcgat acggacggtc acgtatacgt cacagtgcca
     1201 gggctattct ctcctccgaa cgggccgttc gcgtttccgt gtccgctcag tgcagcaggg
     1261 actttcgttt gctccggcct cgttgcgctc gcggagtcgg cgggcgtcgt cagcgcgcag
     1321 ctcgtcgacc ctccgtcgag ggttggaagc acttcccttg ccgtgaccgg ctcggtgaac
     1381 cctgccggcg gtgtcgctcg ctaccggttc gaggtgtcga ccgacgggtc cgagtggcgg
     1441 gacacgggcg tcggcggcta cgcggccggc acggcggacg tggaggcccc gggcgagctc
     1501 tccgccctgc agcccgcgac cccctacaag gtgcggctgc gtgccgagaa gcacacgagc
     1561 atcacgactg tgcagtcggc gagctcgaac gaggtcacga ccgtgacggc gatcgctccg
     1621 cctgacgtga gcacccgccg ggcggtgtac gtcaccgaca cgtcggccga gctccgggcg
     1681 cggatcgacc cgaacggcat cgagacgacg taccgcttcg agtacgggtt ggccggcggg
     1741 ccgctggatc gccaggtccc gctcaccgat gcgggtgctg gcaccggcaa cgatccgcag
     1801 ctcttcgtgg aggatctcgc gggcctcgag ccggccaccg cctacgcgtt ccggatagtg
     1861 gcgacgaatg cggccggcga gtcggtcgga gcgacggtcg cgttcgagac ccgggagctc
     1921 gacgacgtct acgctccgcc gggacgcggc tatgagctcg tctcgccggc ggacaaggtc
     1981 gccggcgtcg gcaccggcgt ctggtacaac gggcccgcgg cgatcgagga ggtcggcttc
     2041 ggcgcgcacc gcggcgagcg gttcgcggca aaagggacgc ttggcgcgac cctgctcgac
     2101 ggcccgtatg cgtacgccaa cgactgggcg ctggccgagc ggaccgacag cggatggaag
     2161 agcggcccgg gcgcgtcgcg gcgcgcgtat ggcagccagc acaagacctt catgaccatg
     2221 tccagcgcga cccccgacct gtcgacgatg ggctggacca gcaacgggca cacggtgaag
     2281 cccttccctg agatggagcc gtgggaggag acgcgggtgg gcaacgtgct gctgctgcgc
     2341 cgctgggcgg ggggcgagtg ggagctcttc ggaccgctcg cggagggcca tgtgaccgac
     2401 aaccagcccg agctctccga ggcggtcgcc gaggacggga ccgccgtggc ggcgaccgcg
     2461 ttgagggttc gcggcctggc cggcgcgggc gatccgacga gcccggcttt cgacgacctc
     2521 gtggagggct cgaacgtcta cgtcgacgag acgccggggg ccttctccga cgtcttcccg
     2581 ggcgacgacg gcgtccgcga gctggtgaac gtgtgcacgg ccgggacggt ccttccgacc
     2641 agggtgcagg caggtgcctt cttcaagcag ggcacggctg cctgtcccga cgcgctcgcc
     2701 ggccgtgact cgcgcctggt gagcccgtac ggggcgtcgc tcggcaccga caaggagggc
     2761 gtgatctccg cggacgggtc acgggtgttc ttcatggcac ccgacgtccg ggtcccgcag
     2821 tcgaccgccg gctgctcggg cgtcggcgcc gataccgtct gcccgactca gctgtacgtc
     2881 cgtcagcgca gctccgacgg ctccgtcgtg acccgctgga tctctcggac gcaggtgacc
     2941 gcggcaaacg gcgcggccgc cgaccaggac gcgtcgctga tcggtccggc gttcttcgag
     3001 ggagcgtccg ccgacggcga caaggtgttc ttccgcacaa actcgccgtt gaccgccgac
     3061 gacccgaacg gcttggggag cgcccctgcc ggcggagtca cgaccggcga cgcgcacggc
     3121 gagtcgtggg acctctacat gtacgacatg cccgacgggc aggacgccga cccggccggc
     3181 ggggagctgg tgcggatctc ccgcggcccc ggcggccagg gcgactgcaa cagcccgttc
     3241 ggcggggcgg gcagcgaccg gccgggcgcg ctgcggttcg tctctgctga cggggcgcgg
     3301 gcgttcttca cctgtgccgc cccgctcccc ggggtcgcgg acccgggcag cggcacgatc
     3361 accgatccca ccggcgcgcg cgacacggcc gcgacgtcga acctctacgt ctatgacgct
     3421 ggccggtcgg gcgccgaccg ctggcggttc gtcgcgctgc tgccgcgcac cagcccgctc
     3481 ggcgcgtgcg cgacgcggtc cgctgagctc gggtcgacgc tctacgggtc gggcaacgac
     3541 gtgaatctct cgaacgtggg cagctgcgtg agcgggacgg ccgacggggc gttcgtgacc
     3601 ttcttcaccg acggcgcgtt gacgatcgac gatcccgacg acggcgtcgg cgacgtctac
     3661 gggtactcgg cgacgcgcga tcagctgcac cggctgtcga aggcacaggg tggcgccggg
     3721 ggcgcgtatc cctgccggcc tcagacaccc gccccgatgt gccacggcga tggcggcatc
     3781 ggcccggaga gaatgccggt caagcgcctg ggggtggcct cggagcccac gaaccagacg
     3841 gatcggattg cctacttcca gtcgcgcagc cagctcgttg cggaggacac cgatgacgcg
     3901 tacgacgtct acgagtggcg caacggcgac ctgacgctgc tgtcgacggg gcagagcccg
     3961 accgacgggg ccttctacgt cggcaacgac gcgaccggcc gcaacgtctt catggcgacg
     4021 cgcgatcgcc tgtcctggga ggactcagac gcggtgctcg acgtgtactc cgcccgcgcc
     4081 ggcgggggct tcgaccggcc gccagtccac cggccgtgcg acgtcctcgg cgatggctgc
     4141 cagggcacgg gcggatccgc cgtcggcccg ccgatcggct cggatgcgcc gggcggctcc
     4201 aacgcgaagc cgcagaggcg gatcgccctc acgctgcgga cgcatctgtc gcgtcgcgcg
     4261 cgacagctcg cggctcgacg tggcgtggta ccggtgaggg tccgcgtgag cgggccgacg
     4321 ctggtgcggg tggccgccag agcgcctgtc ggcgggcggc tgcggagggc aggcacggcg
     4381 cgcaagcggg tgcacaaggc tggcacggtg acggtccggc tgcgcgtgcg cggccaggct
     4441 cggcgccggc tgcgctcggg cagggccgtc cggctgcggt tggtcgtgag ggcgaagggc
     4501 gcggtgcccg agaaggtgac gatcgtcctg aagagaggtc ggcggtgagc ggacgggcaa
     4561 ggcaacggaa cggggctgag atggctggaa ctggcaaggt tggtcggcgg cgcgtcttgg
     4621 tcgtcgcgct cgcgctgatg gcgctgctgt ccgcggtgac ggcgtcgcag gcgcgtgccg
     4681 ccttcggcga cctgttcggc ctcgcgccgg tgaacggccc cggcctgccg gcctcgccgg
     4741 tctttcccgc cacagccggt gtcgaccacg ccttctgggc gggcacctgc gacgtcgggt
     4801 cggcgccgtt cggaccgatc gccggcggcg tcggcagccg ccc
//
LOCUS       DATABV010000100         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1234606, whole genome shotgun sequence.
ACCESSION   DATABV010000100 DATABV010000000
VERSION     DATABV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234606"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VHF88_04255"
     CDS             complement(<1..526)
                     /locus_tag="VHF88_04255"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX2161016.1"
                     /translation="MAGDIMLGRRVGDAAAAAGDVSAPLRPLQRRLARADVTVGNLES
                     TLSRAGAPRQGDDSFAAPPGVLRGLADAGFDVLSLANNHTGDFGRRALLDTLRRIDRS
                     PVERVGAGRRRAGAWRPAVVERNGVRFGFVAFNAIGETPRATPTRPGAAEVRMQPRTG
                     PLSAADLPRAQRYIR"
     gene            869..>2466
                     /gene="topA"
                     /locus_tag="VHF88_04260"
     CDS             869..>2466
                     /gene="topA"
                     /locus_tag="VHF88_04260"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX2161017.1"
                     /translation="MAANTSADANGVRRLVIVESPAKARTIAGYLGRGYVVESSIGHI
                     RDLPQSAADIPAKYKGEPWARLGVNVDDDFAPIYVVPAKQKPQISKLKKLLKDSDELF
                     LATDEDREGEAIAWHLFDELKPKIPVRRMVFHEITEHAIRDAVDNARDIDERLVDAQE
                     TRRILDRLYGYEVSPVLWKKVMTGLSAGRVQSVATRLVVDRERERIAFRTARYWDLEA
                     TFDAGESRDPRTFPAKLVAVDGARVAQGRDFAPTGELKSGSGVVHLDGDRAGALATGL
                     RDRTFAVRSVESKPYKRSPYAPFRTTTLQQEASRKLGYGASRTMQVAQRLYENGFITY
                     MRTDSVTLSETAIAAARDQVRELFGSEYLPEAPRTYQSKVKNAQEAHEAIRPAGDAFR
                     TPAQTGLAGDEFRLYELIWMRTVASQMKDAQGRTVTVRLGAQASTGEECEFSASGRVI
                     TFHGFLKAYVEGADDPAERDDRETRLPDVSEGDRVDVASLEPAEHQTKPPARYTEATL
                     VRELEQREIGRPSTYASIIGTILNR"
BASE COUNT          364 a          907 c          886 g          309 t
ORIGIN      
        1 cccggatgta ccgctgcgca cgcggcaggt cggcggcgct cagcggcccg gtccgcggct
       61 gcatccgcac ctcggccgcg cccggacgcg tcggggtcgc ccgcggcgtc tccccgatcg
      121 cgttgaaagc gacgaagccg aaccgcacgc cgttgcgctc gaccaccgcc gggcgccagg
      181 ccccggcccg ccgccggccg gcgccgacgc gctcgacggg gctgcggtcg atccgccgca
      241 gcgtgtccag cagtgcccgg cggccgaagt cgccggtgtg gttgttggcc agcgagagga
      301 cgtcgaagcc ggcgtcggcg agcccgcgga gcacgcccgg cggcgcggcg aaggagtcgt
      361 cgccctgccg tggcgcgccg gcgcgcgaca gcgtgctctc caggttgccg acggtgacgt
      421 cggctcgcgc caggcgtcgc tgcagcggcc gcagcggggc gctcacgtcg ccggccgccg
      481 ccgcggcgtc cccgacccgc cggcccagca tgatgtcgcc ggccacggtg accctggtgc
      541 ggaccggtcg ctgctcgcgg gctcgcgggg tcggggtcgt ttcgctcgac ggcgtactct
      601 cgggagcaga ccgcgcatcg cccggcgtgg ggctgggcac cggggaggtg ggacgggcgc
      661 tgggcgcggg cttcgcggac cgctccgtgg gctcgctcgt gcaggcgacg aggcccatcg
      721 ccagcgccgc cagggcgcac cacgcgcgaa cacgcatgcc cttgacggta cgtgcaacca
      781 cccagcacca aggggttcgg aaccgctcgg agggcccgac ccgttaccgt tcatcgggac
      841 tcacacggct tcaaggagaa gaacagcagt ggcagcgaac acctcggccg acgccaacgg
      901 cgtccggcgg ctcgtcatcg tcgagtcgcc ggccaaggcg cgtacgatcg cgggctacct
      961 cggccggggg tacgtcgtcg agtcgtcgat cggccacatc cgtgacctgc cgcagagcgc
     1021 cgccgacatc ccggccaagt acaagggcga gccgtgggcg cgcctcggcg tcaacgtcga
     1081 cgacgacttc gcgccgatct acgtcgtgcc ggccaagcag aagccgcaga tcagcaagct
     1141 gaagaagctg ctgaaggact ccgacgagct cttcctcgcc accgacgagg accgtgaggg
     1201 cgaggccatc gcctggcact tgttcgacga gctgaagccg aagatcccgg tgcgccggat
     1261 ggtcttccac gagatcaccg agcacgcgat ccgcgacgcc gtggacaacg cccgcgacat
     1321 cgacgagcgg ctggtcgacg cgcaggagac ccgccgcatc ctcgaccggc tctacggcta
     1381 cgaggtctcc ccggtgctgt ggaagaaggt catgacgggc ctgtccgcgg gccgggtgca
     1441 gtcggtggcg acccggctgg tcgtcgaccg cgagcgcgag cgcatcgcgt tccgcaccgc
     1501 ccgctactgg gacctcgagg ccaccttcga cgccggtgag tcccgcgacc cgcggacgtt
     1561 cccggccaag ctcgtcgccg tcgacggcgc ccgggtggcg cagggccgcg acttcgcgcc
     1621 cacgggggag ctgaagagcg gctccggcgt ggtgcacctc gacggcgacc gcgccggcgc
     1681 gctggcgacc ggcctgcgtg accggacgtt cgcggtccgc tcggtcgagt ccaagccgta
     1741 caagcgctcg ccgtacgcac cgttccgcac caccaccctg cagcaggagg cgtcccgcaa
     1801 gctcggctac ggcgcctccc gcaccatgca ggtcgcgcag cggctgtacg agaacggctt
     1861 catcacctac atgcgtaccg actcggtcac gttgtccgag accgcgatcg cggcggcccg
     1921 cgaccaggtt cgggagctgt tcgggtcgga gtacctgccc gaggcgccgc gcacctacca
     1981 gagcaaggtc aagaacgcgc aggaggcgca cgaggccatc cgtccggccg gtgacgcgtt
     2041 ccgtacgccg gcgcagacgg ggctggccgg cgacgagttc cggctgtacg agctgatctg
     2101 gatgcggacc gtcgcctcgc agatgaagga cgcccagggg cgcaccgtga ccgtccgcct
     2161 cggggcacag gcctcgacgg gtgaggagtg cgagttctcc gcctccggtc gcgtcatcac
     2221 gttccacggc ttcctcaagg cctacgtcga gggtgccgac gaccccgccg aacgcgacga
     2281 ccgcgagacc cggctcccgg acgtgtccga gggcgaccgg gtcgacgtgg cctcgctgga
     2341 gccggccgag caccagacca agccaccggc ccgctacacc gaggccacgc tggtgcgtga
     2401 gctggagcag cgggagatcg ggcgcccgtc gacgtacgcc tcgatcatcg gcacgatcct
     2461 caaccg
//
LOCUS       DATABV010000101         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1877803, whole genome shotgun sequence.
ACCESSION   DATABV010000101 DATABV010000000
VERSION     DATABV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877803"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VHF88_04265"
     CDS             <1..379
                     /locus_tag="VHF88_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161018.1"
                     /translation="EIKDRNNGYGHEKAFTTLIEKYGTLHEAQLLPRSFGDGSLIKGQ
                     TEPGAVKELIENLPTAIRGLKSGKVTIGKALMHHKLPDQKNVKRIYETIESKDERVEL
                     NLYIVGETGSEEQGAMSEGGGES"
     gene            376..1278
                     /locus_tag="VHF88_04270"
     CDS             376..1278
                     /locus_tag="VHF88_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEX2161019.1"
                     /translation="MKLAYWPGCVSRGFTPELHGSMNLVAPKLDLELVELDRANCCGA
                     GVIAEHNQELADTLNARTFAMAQGVEGAVGMMNICSTCQGAQSECQQRLDADGDYREI
                     VNSNLSAENLAYQQHSEWWNKNFLWLLVEEIGLDKLRSKVVRPLEGLRIAPFYGCYIV
                     RPTKRLGYADHPERDQYLDYVIEALGAQPVEYAGARKCCGFPVITMNRTTSLRQAGRH
                     LADAIDAGADCLVTPCPLCHLNLDMQQPAAAKFVERDLDVPVLHLPQMVGLALGFEPK
                     ELGMNKHVVKTKDVQRKIAELAAA"
     gene            1627..2103
                     /locus_tag="VHF88_04275"
     CDS             1627..2103
                     /locus_tag="VHF88_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX2161020.1"
                     /translation="MSAELRLRELLERLVAADVRFVLVGGLAVNAWGYLRATQDVDIV
                     PDPEPANLERLAALLEGLDGKVEVEGGRLKSSAIRTFLKAGDRTLVSTKAGHVDVLQG
                     LPQVPPFSQLDGDGESVDLDGITVKICSLDALKAMKAASERARDRDDLEALEALEE"
     gene            complement(2124..3146)
                     /locus_tag="VHF88_04280"
     CDS             complement(2124..3146)
                     /locus_tag="VHF88_04280"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX2161021.1"
                     /translation="MRVAVVGATGAVGSTILGIMRERDFPADEVVPFASERSAGRAID
                     WGDQNLTVVPLTDETIQGFDLALFSAGSGVSEQWGPRFAAAGATVVDNSSYWRMQDDV
                     PLVVAEVNPDALADHKGIVANPNCSTMQMVVALKPIVDAAGIERLVISTYQAVSGTGQ
                     RAVEELHDQTRAVLEATEVPQPQIYPHQIAFNAMPQVESFKDGDDYTTEERKMMAETR
                     KILGRDDIAISATCVRIPVYTGHSESVNVQTSKALSPEDCRTLLSDAPGVQVLDDPAN
                     GIYPVAIDAAGRDDVLVGRIRNDPSHPNCLNLWIVGDNLRKGAALNAVQLAELLTTRN
                     LLRTAA"
     gene            complement(3149..>4201)
                     /locus_tag="VHF88_04285"
     CDS             complement(3149..>4201)
                     /locus_tag="VHF88_04285"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX2161022.1"
                     /translation="MAINDLGREAISLTGSQAGIVTDASHTKARILDVRADRIREALD
                     GGKVVLVAGFQGVSTDTSDVTTLGRGGSDTTAVALAAALNADVCEIYTDVPGVFSADP
                     RIVPDARKLPVVSFEEMLEMAASGAGVLQLRSVEYARNNGIPIHCRSSFDDGPGTFVV
                     DEQQTMERPLVTAVTHSTDEARVTLTGVPDQPGIAGRVLTALAEANVNVDMIIQNEPE
                     SDGARADMSFTVPRADLTAALDALEPIRADLGIAGIASDPAMAKVSIVGAGMRSHPGV
                     AAKTFSVLGEAGVNIEMIATSPIKISCVIREEHVADAVRELHTAFDLGADAVLPEDVS
                     GDHRPVVKSTAETSEA"
BASE COUNT          660 a         1240 c         1614 g          687 t
ORIGIN      
        1 cgagatcaag gaccgcaaca acggctacgg ccacgagaag gcgttcacga cgctgatcga
       61 gaagtacggc acgctgcacg aggcgcagct gctgccgcgc tcgttcggcg acggctcgct
      121 gatcaagggc cagaccgagc ccggcgcggt caaggagctg atcgagaatc tgccgaccgc
      181 aatccgcggc ctgaagtccg gcaaggtgac gatcggcaag gcgctgatgc accacaagct
      241 gcctgaccag aagaacgtca agcggatcta cgagacgatc gagtccaagg acgagcgcgt
      301 cgagctgaac ctctacatcg tcggcgagac cggcagcgag gagcagggcg ccatgtcaga
      361 gggaggtggc gagtcatgaa gctcgcgtac tggccgggct gcgtgtcacg cggcttcacc
      421 cccgagctgc acggctcgat gaacctcgtc gcgccgaagc tcgatctcga gctggtcgag
      481 ctcgaccgcg ccaactgctg cggcgccggc gtgatcgccg agcacaacca ggagctcgcc
      541 gacacgctca acgcgcgcac cttcgcgatg gcccagggcg tcgagggcgc ggtcgggatg
      601 atgaacatct gctcgacctg ccagggcgcc cagtccgagt gccagcagcg gctcgacgcc
      661 gacggcgact accgcgagat cgtcaactcg aacctttccg ccgagaacct cgcctaccag
      721 cagcacagcg agtggtggaa caagaacttc ctctggctgc tggtcgagga gatcgggctc
      781 gacaagctgc gctcaaaggt cgtgcggccg ctcgaggggc tgcggatcgc gcccttctac
      841 ggctgctaca tcgtgcggcc gaccaagcgg ctcggctacg cggaccatcc cgagcgcgac
      901 cagtacctcg actacgtgat cgaggcgctc ggcgcgcagc cggtcgagta tgcgggggcg
      961 cgcaagtgct gcggcttccc ggtgatcacg atgaaccgga cgacttcgct gcgtcaggcg
     1021 gggcggcatc tcgccgacgc gatcgatgcg ggggcggatt gcctggtgac cccctgtccg
     1081 ctgtgccacc tgaacctgga catgcagcag cctgcggcgg cgaagttcgt ggagcgggat
     1141 ctcgacgtgc cggtgctgca cctgccgcag atggtggggc tggcgctggg gttcgagccg
     1201 aaggagctcg ggatgaacaa gcacgtcgtc aagacgaagg acgtgcagcg gaagatcgct
     1261 gagctggctg ctgcttagtc gcgcggctgc tcttctgatt aaggatcgac cgctgggccc
     1321 gcgggtcctg tctccggttt tggtttacgt gctcgcaagc tgcgcgcgca aacgcaaaac
     1381 cgtcgccacc cgcgggaagg ggcgtcggtg gactgcttgg aagtgcggcg gggcttaggg
     1441 ttgtgatgtg gcggagcgtg agaaagggcg ggaagcgagg gagcgggagc gcgatccgtg
     1501 ggctctcaac gaggtcgtgc gggttcggag gattcgggag gatgggaagc gtccgatggc
     1561 tgtgaacctt gcggaggggt tggctctcag tgagttcatg gcgaagttca agggcgctgc
     1621 gaggcggtga gcgcagagct tcggctgcgt gagctgttgg agcggttggt cgccgcggac
     1681 gtgcggttcg ttcttgtcgg tggccttgcg gtcaatgctt ggggctatct gagggctact
     1741 caggatgtgg acatcgtgcc ggatccggaa ccggcgaact tggagcggtt ggccgctctg
     1801 ctcgagggtc tggacgggaa ggtcgaggtc gaaggcggcc gcctgaagtc gagcgcaata
     1861 cgcacgttct tgaaggcggg agaccgtacg ttggtgagta cgaaggcggg ccatgtcgac
     1921 gtcctgcagg gtcttccgca agtcccccct ttttctcagc tagatggtga tggggaatcc
     1981 gtagatctcg acgggatcac cgtcaagatt tgctcgctcg acgccctgaa ggcgatgaag
     2041 gcggcatcgg agcgagctcg ggaccgggac gacctcgagg cgctggaggc cttggaggag
     2101 tagtcgcccg accgcaatcg cgctcaggcg gcggtgcgga ggaggttgcg ggtggtgagg
     2161 agctcggcga gctggacggc gttgagggcg gcgcccttgc ggaggttgtc gccgacgatc
     2221 cagaggttga ggcagttggg gtgagagggg tcgttgcgga tgcggccgac gaggacgtcg
     2281 tcgcggccgg cggcgtcgat cgcgacgggg tagatgccgt tggcggggtc gtcgaggacc
     2341 tggacgccgg gggcgtccga taggagcgtg cggcaatcct cgggtgagag ggccttgctg
     2401 gtctggacgt tgacggactc ggagtggccg gtgtagacgg gaatgcggac gcaggtggcg
     2461 gagatcgcga tgtcgtcgcg gccgaggatc ttgcgcgtct cggccatcat cttgcgctcc
     2521 tcggtggtgt agtcgtcgcc gtccttgaac gactcgacct ggggcatcgc gttgaaggcg
     2581 atctggtgcg ggtagatctg cggttggggg acctcggtcg cctcgagcac ggcgcgggtc
     2641 tggtcgtgga gctcctcgac ggcgcgctgg ccggtgccgg agaccgcctg gtaggtcgat
     2701 atcacgaggc gctcgatgcc ggcggcatcg acgatcggct tgagcgcgac gaccatctgc
     2761 atcgtcgagc agttggggtt ggcgacgatg cccttgtggt cggcgagcgc gtcggggttg
     2821 acctcggcga cgacgagcgg gacgtcgtcc tgcatgcgcc agtaggagga gttgtcgacg
     2881 acggtcgcgc cggcggcggc gaagcggggg ccccactgct cgctgacgcc tgagccggcg
     2941 gagaagagcg cgaggtcgaa gccctggatc gtctcgtcgg tcaacgggac caccgtcaaa
     3001 ttctggtcgc cccagtcgat cgcgcgaccg gcggagcgct cggaggcgaa cgggacgacc
     3061 tcgtcggcgg ggaagtcgcg ctcgcgcatg atcccgagga tcgtcgagcc gaccgcgccg
     3121 gtggccccta cgacggctac gcgcacggtc aggcctccga ggtctcggcg gtcgacttga
     3181 cgaccgggcg gtggtcgccg gagacgtcct cggggaggac ggcgtcggcg ccgaggtcga
     3241 aggcggtgtg cagctcgcgg acggcgtcgg cgacgtgctc ctcgcggatc acacaggaga
     3301 tcttgatcgg tgacgtggcg atcatctcga tgttgacgcc ggcctcgccg agcacgctga
     3361 acgtcttcgc ggcgacgccg gggtggctgc gcatgccggc accgacgatc gacaccttcg
     3421 ccatcgcggg gtcgctggcg atgcccgcga tgccgaggtc ggcgcggatc ggctcgagcg
     3481 cgtcgagcgc ggcggtcagg tcggcgcgcg ggacggtgaa cgacatgtcg gcgcgcgctc
     3541 cgtcggactc cggctcgttc tggatgatca tgtcgacgtt gacgttcgcc tcggcgagcg
     3601 cggtcaggac gcgaccggcg atgccgggct ggtccggcac gccggtgagg gtgacgcgcg
     3661 cctcgtcggt cgagtgcgtc acggctgtca cgagtggtcg ctccatcgtc tgctgctcgt
     3721 cgaccacgaa ggtaccgggt ccgtcgtcga agctggagcg gcagtggatc gggatgccgt
     3781 tgttgcgcgc gtactcgacc gagcgcagct gcaggacgcc ggcgccggac gccgccatct
     3841 cgagcatctc ctcgaacgag acgaccggca gcttgcgcgc gtcggggacg attcgggggt
     3901 cggcgctgaa gacgccgggg acgtcggtgt agatctcgca gacgtcggcg ttcagcgcgg
     3961 cggcgagcgc gactgcggtc gtgtcggagc cgccgcggcc gagcgtggtg acgtcggagg
     4021 tgtcggtcga gacgccctgg aagccggcga cgagcacgac cttgcccccg tcgagggcct
     4081 cgcggatgcg gtcggccctc acgtcgagga tgcgcgcctt ggtgtgcgag gcgtccgtga
     4141 cgatgccggc ctgggagccg gtcagggaga tcgcctcgcg cccgaggtcg ttgatcgcca
     4201 t
//
LOCUS       DATABV010000102         9694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_673019, whole genome shotgun sequence.
ACCESSION   DATABV010000102 DATABV010000000
VERSION     DATABV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673019"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VHF88_04290"
     CDS             complement(<1..383)
                     /locus_tag="VHF88_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2161023.1"
                     /translation="MELASGKLVRDEPADGVVRLRISNPERRGALDHEILDTLAETVA
                     ALDARCLVLTGSGGVFSAGYDLGNLEGREFEENAEKLVAHPFHDAIEALEAYPYPVIA
                     QINGHAIGGGLELALTCDIRIAKRG"
     gene            complement(385..597)
                     /locus_tag="VHF88_04295"
     CDS             complement(385..597)
                     /locus_tag="VHF88_04295"
                     /inference="COORDINATES: protein motif:HMM:NF004547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEX2161024.1"
                     /translation="MPNVEAHITGTVWKIECKVGDEVEEGDTVAILESMKMEMPVEVE
                     DDGTVAEIKCSEGQSVNEGDVLVVLD"
     gene            complement(681..1325)
                     /locus_tag="VHF88_04300"
     CDS             complement(681..1325)
                     /locus_tag="VHF88_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161025.1"
                     /translation="MFLGAAVAAVAALAPAATAAAVEPILFVHGWNSTGSVWNTMIGR
                     FQADGWTAAQTNNWSYNTAQSNVTTAGQVNTKVDQIRAATGAAKVDIISHSMGGLNTR
                     YYLKNLGGAAEVDEWVSLGGPNHGTQTAYACFWNTSCFEMQPGSSFLNNLNATDETPG
                     AVNYGTWWSPCDNVINPDSSVSLIGATNTQTACLGHSDLYLDATVYNQVRNFVD"
     gene            1459..2913
                     /gene="glpK"
                     /locus_tag="VHF88_04305"
     CDS             1459..2913
                     /gene="glpK"
                     /locus_tag="VHF88_04305"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX2161026.1"
                     /translation="MILALDQGTTGSTALVFDAGGRIAGRAYSEFRQHFPRPGWVEHD
                     AEEIWQVTRRVGREALDAAGIDGRALAGIGIANQRETVVAWDRSSGEPLHRALVWQDR
                     RTAARCDELRAAGHEPLVRERTGLVLDPYFSGTKIEWLIRHGGVDPARAAFGTVDSWL
                     VHRLTGRHATDYSNASRTLLFDIGQLRWDGELCELLGVPLDALPEPCPSAHVYGETSE
                     FGGSVPVAGIAGDQQAALYGQGCHAPGLGKNTYGTGSFVLLNAGEERPAPQEGLLTTL
                     AWGVDGRVDYALEAAIFVTGAAVQWLRDGLGVIRTAAETEELARSLDSNDGVYLVPAF
                     TGLGSPHWDPYARGTIVGLTRGSGRAHLARATLEAIAYQTVDAVRAMETASGIDFAEL
                     RADGGAVHNSWLMRFQADVLGVPVIVPEVSETTALGAAYLAGVATGTWTEADVRAMWR
                     EAARYEPRMPAEQRDDLLAGWRRALERSRGWAGA"
     gene            2946..3656
                     /locus_tag="VHF88_04310"
     CDS             2946..3656
                     /locus_tag="VHF88_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2161027.1"
                     /translation="MADVKLKEQVYKDPRPAEHFDRYHRRVREHRPNWDYELVRTILT
                     PYLLGFFRTRAFDTYNVPADGPCILAPNHFSFLDHFFLAVYLRRKVHFMAKSQLFKLP
                     LWPFFNYGGVFPVRRGHADQESFDTAHAILARGDIVVMYLEGGRSRTGELGTPRPGVG
                     RLALETGVPVVPVAIVGSEKTRNWKRGQFPKVSVRFGAPVRFDPVDEPTRDQAQYASE
                     QVFERIRGLWQDARDSGR"
     gene            complement(3653..4555)
                     /locus_tag="VHF88_04315"
     CDS             complement(3653..4555)
                     /locus_tag="VHF88_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX2161028.1"
                     /translation="MATAAEPRPARLPLPGGREGATVRLHPLLCARMVAPPGLIHRTD
                     GRLAPLRALGVGKSQDEWPELPVQAFLVEHPGAGLVLVDTGFHAAVAVDPRQGMGRLG
                     GLIYKDLRMDTAEAVPDQLRQRGFDPGDVATVVLTHLHSDHASGIAQFPQATFVVSSA
                     EWEAASEGGVTDGYIKRQFDHAFDYRLLDFESEAADSFETFGRSLDLFGDGSLRIVFT
                     PGHSPGHCSVVLRLGDRDALLTGDAAYTHQTIAETALPYRMADEHRFRRSLREIQRFI
                     EQRPDTLVIPAHDMAAFRRLRETY"
     gene            4593..5441
                     /locus_tag="VHF88_04320"
     CDS             4593..5441
                     /locus_tag="VHF88_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2161029.1"
                     /translation="MGSEIFRPGLLEGQVALVSGGGSGLGRRSAVELAELGAQVVICG
                     RRADPLAETVALCADGRCSARVCDIREEDQVEALVDAVLADHGRIDLLVNNAGGQFLT
                     PAEEITPKGFRTVIRLNVEGTWLMTHAVATRAMIPGGTGGKIVNVTLSPHNGLPGMAH
                     SSAARAAVENLTRVLSIEWSRYGIKLNALAAGQFGTETLRTKYPQAIVDHLEQTVPLG
                     RLGTEEEFAWAIAYLASPAGDFYSGAVITMDGARDNWFGTWPPRSIADAGGKPLSEER
                     RPRDRG"
     gene            complement(5438..6265)
                     /locus_tag="VHF88_04325"
     CDS             complement(5438..6265)
                     /locus_tag="VHF88_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2161030.1"
                     /translation="MGVLDGKSAIVTGSARGIGRATAELLASQGARVLVNDLDGDVAE
                     QAAGEIAGETVAFAGDLTKDGVPDKLVETAVEAFGGLDIVVNNAGYTWDGPIHKMSDE
                     QFQAMLDIHNVVPFKVLRAAAPHMREPAKKEREEGREVFRKVVNVSSVSGTMGNAGQA
                     NYSSGKSGVVGLTKTLAKEWGQFKINVNAVAFGFIETRLTASKENAETIEAEGKEIQL
                     GIPEQLRQMATMLIPMGRPGTPEEAAGGVFFLCSPWSNYVHGQVLNVTGGQFTGMTS"
     gene            6352..6897
                     /locus_tag="VHF88_04330"
     CDS             6352..6897
                     /locus_tag="VHF88_04330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX2161031.1"
                     /translation="MATTALAVVDMLNPYDHEDAEVLAESVEDVVEPIRVLLDHAGDD
                     DVDVIYVNDNYNDWNSSQEELARRALDGRRPDLIEPILPPEESSFVIKARHSIFYGTP
                     LEHLLHEREIDRLVLCGQVTEQCILYSALDAYVRQFDVSVVGDAVAHIDRSLADAALR
                     MMERNMRAQVVESRKFHSLLC"
     gene            6907..8094
                     /locus_tag="VHF88_04335"
     CDS             6907..8094
                     /locus_tag="VHF88_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161032.1"
                     /translation="MATAETVLKPDFGSKRKHFIFEEHHDRLRESIRSFVEKELTPHA
                     DEWEETTFPDSVIRRMGELGFLGLSMPEEYGGQGGDYFCNLVLAEEMGRSNSGGLTMG
                     VAVHTDMAMLPIHLFGSEEQKQQWLVPAIKGEKILCLGITEPDAGSDVAGIKTRAVRD
                     GEDYVINGSKTYITNGHRADVIVLVTKTDPDAGYDGFTLFLVPMDAPGVIREKKLEKL
                     GMHASDTALLAFQDVRVPASAMLGQEGKGFYHIMWELQGERLIGAAGCVAGAQRCFDK
                     TLQYALEREAFGRPIGKFQAIRHKFAEMATKIEAGRQMTYTTAWRFNNGEYPVREISM
                     AKLFTAQMACEVTDECLQIHGGAGYMREYGIERAWRDMRLNRIGAGSDEIMLDVIGRS
                     YGL"
     gene            8099..8380
                     /locus_tag="VHF88_04340"
     CDS             8099..8380
                     /locus_tag="VHF88_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161033.1"
                     /translation="MRLEPPFTDEHQALRESIRRFVEKELRPYAAQWEDERWFPNDVF
                     GKLAANGFLGLKYPERYGGEGGDHVHDAVLIEELGGCGSGGVAAGVVRT"
     gene            8440..9246
                     /locus_tag="VHF88_04345"
     CDS             8440..9246
                     /locus_tag="VHF88_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161034.1"
                     /translation="MPGIRGEKIAALGITEPGTGSDVAGIRTRAKRVDGGWVVNGSKT
                     FITGGVRADVVVTAVKTSDEGGHGGLSFLLIEKGMEGFTVSKKLEKLGWHASDTAELA
                     FQDVFVPEENLLGDENRGFYLIMANFQWERIAMALGAVAAMQRLLDTSVAYALEREAF
                     GRPIGRFQAIRHKVAQMAVKLEASRALTYHALRLFHDGQDAIREVTMAKLLSQRAACE
                     VADEAIQIHGGAGYMREYEIERAARDLRLGPIGGGTDEIMKEILGRQMGL"
     gene            9275..>9694
                     /locus_tag="VHF88_04350"
     CDS             9275..>9694
                     /locus_tag="VHF88_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2161035.1"
                     /translation="MTERAFDAIVLGAGPAGEVVAGRLADGGLSVAIVEPHLVGGECS
                     FYACMPSKALLRPGELLAEARGVPGVAEAIAGDLDATATLARRDEVIHDLDDSGQLPW
                     LEDKGIELLRGEGRLDGERRVRVGDELLIARRAVVVAT"
BASE COUNT         1457 a         3350 c         3395 g         1492 t
ORIGIN      
        1 atgccgcgct tcgcgatccg gatgtcgcag gtcagcgcca gctcgagtcc gccgccgatc
       61 gcgtggccgt tgatctgcgc gatcaccgga tagggatagg cctcgagcgc ctcgatcgcg
      121 tcgtgaaacg ggtgggcgac cagcttctca gcgttctcct cgaactcgcg cccctcgagg
      181 ttgccgaggt cgtagccggc cgagaagact ccaccgctgc cggtcaggac gaggcagcgc
      241 gcgtcgagcg cggcgaccgt ctcggcgagg gtgtcgagga tctcgtggtc gagcgcgccg
      301 cgccgctcgg gattcgagat ccgcaatcgc acgacgccgt ccgcgggctc gtcgcggacc
      361 agcttgccgg aggcgagctc cacgctagtc gagcacgacg agcacgtcgc cctcgttgac
      421 cgactggccc tcggagcact tgatctcggc gaccgtgccg tcgtcctcga cctcgaccgg
      481 catctccatc ttcatcgact cgaggatcgc gaccgtgtcg ccctcctcga cctcgtcgcc
      541 gaccttgcac tcgatcttcc agaccgttcc ggtgatgtgc gcctcgacgt tcggcaatgc
      601 gtctccttgc tcggtggggt cgcgcggcgg caatgtaccg ccaccgcgcg accgatcacc
      661 gagccagagc gcctctggga tcaatccacg aagttgcgca cctggttgta gaccgttgcg
      721 tccagataca ggtcgctgtg gcccaggcag gccgtctggg tgttcgtcgc accgatcagc
      781 gagacgctgc tgtccgggtt gatgacgttg tcgcacggcg accaccaggt gccgtagttg
      841 accgcgccgg gcgtctcgtc ggttgcgttg aggttgttca ggaacgagga gccgggctgc
      901 atctcgaagc acgaggtgtt ccagaagcag gcgtaggcgg tttgggtgcc gtggttcggc
      961 ccgccgagcg agacccactc atcgacctcc gcggctccgc cgaggttctt gaggtagtag
     1021 cgcgtgttga gcccacccat cgagtggctg atgatgtcca ccttggcggc gcccgtggcc
     1081 gcgcgaatct ggtccacctt ggtgttgacc tgaccggcgg tggtcacgtt cgactgtgcg
     1141 gtgttgtagc tccagttgtt cgtctgcgca gcggtccagc cgtcggcctg gaagcggccg
     1201 atcatcgtgt tccagacaga gccggtgctg ttccagccgt ggacgaacag gatcggctcg
     1261 accgccgcgg cggtagccgc cggcgcgagt gctgcgacgg cggctacggc cgcccccaag
     1321 aacatcttcc gcatgattcc ctcctgtaca tgcgttgaca atatgagcga caaaccgtaa
     1381 cccccgtgtc aattttcgga agtgctctga catgtgtgac acgatggcga tagcgtctcc
     1441 gacatctagg atgcgcgcgt gatcctcgcg ctcgaccagg gcacgaccgg ctcgaccgcg
     1501 ctcgtcttcg acgccggcgg ccggatcgcc ggccgcgcct acagcgagtt ccgccagcac
     1561 ttcccgcgcc ccggctgggt cgagcacgac gccgaggaga tctggcaggt gacgcgccgc
     1621 gtcggccgcg aggcgctcga cgcggcgggg atcgacggcc gcgcgctcgc cgggatcggc
     1681 atcgccaacc agcgcgagac cgtcgttgcc tgggaccgct ccagcggcga gccgctgcac
     1741 cgcgcgctgg tctggcagga ccgccgcacg gccgcccgct gcgacgagct gcgcgcagcg
     1801 ggacacgagc cgctcgtccg cgagcgcacc ggcctggtgc tcgatcccta cttctccggg
     1861 acgaagatcg agtggctgat ccgccacggc ggcgttgacc ccgcgcgcgc ggcgttcggc
     1921 accgtcgact cgtggctcgt ccacaggctc acaggtcgcc acgcgaccga ctactcgaac
     1981 gcctcgcgca cgctgctctt cgacatcggc cagctgcgct gggacggcga gctctgcgag
     2041 ctgctcggcg tgccgctcga cgcgctgccc gagccctgcc cgagcgccca cgtctacggc
     2101 gagacctcgg agttcggcgg ttcggtcccg gtcgccggga tcgccggcga ccagcaggcc
     2161 gcgctctatg gacagggctg ccacgcgccg gggctcggca agaacaccta cggcacgggc
     2221 agcttcgtgc tgctcaacgc gggcgaggag cggcccgcgc cgcaggaggg cctgctgacg
     2281 acgctcgcct ggggcgtcga cgggcgcgtc gactacgcgc tggaggcggc gatcttcgtc
     2341 accggcgctg cggtccagtg gctgcgcgac gggctcggcg tgattcggac ggcggccgag
     2401 accgaggagc tcgcccgctc gctcgactcc aacgacggcg tctacctggt gcccgccttc
     2461 accgggctcg gctcgccgca ctgggatccc tacgcgcgcg gcacgatcgt cgggctgacg
     2521 cgcggcagcg gccgcgccca cctcgctcgc gcgacgctcg aggcgatcgc ctaccagacc
     2581 gtcgacgccg tgcgggcgat ggagacggcg tccggcatcg acttcgccga gctgcgggcc
     2641 gacggcggcg cggtccacaa ctcgtggctg atgcgcttcc aggccgacgt cctcggcgtc
     2701 ccggtgatcg tgcccgaggt ctccgagacg accgcgctcg gggcggcgta tcttgccggc
     2761 gtggcgaccg ggacctggac cgaggccgac gtccgcgcga tgtggcgcga ggcggcccgc
     2821 tacgagccgc ggatgcccgc cgagcagcgc gacgacctgc tcgccggctg gcgacgagcg
     2881 ctcgagcgct cgcgcggatg ggccggcgcg tgaccaagcg ccgggcgcgg ggtacctgac
     2941 ggcgcatggc cgacgtcaag ctcaaggagc aggtctacaa ggacccccgc ccggcggagc
     3001 acttcgaccg ctaccaccgg cgcgtccgcg agcaccgacc gaactgggac tacgagctcg
     3061 tccgcacgat cctcacgccc tacctgctcg gcttcttccg cacgcgcgcc ttcgacacct
     3121 acaacgtccc ggcggacggg ccctgcatcc tcgcgccgaa ccacttctcg ttcctcgacc
     3181 acttcttcct cgcggtctac ctgcgccgca aggtccactt catggcgaag tcgcagctgt
     3241 tcaagctgcc gctttggccc ttcttcaact acggcggcgt cttcccggtg cgtcggggcc
     3301 acgccgacca ggagtccttc gacaccgcgc acgcgatcct cgcccgcggc gacatcgtcg
     3361 tgatgtacct cgagggcggt cgctcgcgca ccggcgagct cggcacaccg cgcccgggcg
     3421 tcggccgcct ggcgctcgaa acaggcgtcc cggtcgtccc ggtcgcgatc gttgggtcgg
     3481 agaagacgcg caactggaag cgcggccagt tcccgaaggt cagcgtccgc ttcggcgcgc
     3541 cggtccgctt cgacccggtc gacgagccga cccgggacca ggcgcagtac gcctcagagc
     3601 aggtcttcga gcgcatccgc ggcctctggc aggacgcgcg cgactcgggc cgctagtagg
     3661 tctcccttaa acggcggaag gccgccatgt cgtgggcggg gatcacgagc gtgtcggggc
     3721 gctgctcgat gaagcgctga atctcgcgca gcgagcggcg gaagcggtgc tcgtcggcca
     3781 tccggtaggg gagagcggtc tcggcgatcg tctggtgggt gtaggcggcg tcgccggtca
     3841 gcagcgcgtc gcggtcgccg agccgcagca cgaccgagca gtgtcccggg ctgtgaccgg
     3901 gcgtgaagac gatcctcagg ctgccgtcgc cgaacaggtc gagcgaccgg ccgaaggtct
     3961 cgaacgagtc ggcggcctcg ctctcgaagt cgagcaggcg gtagtcgaag gcatggtcga
     4021 actggcgctt gatgtagccg tcggtcacgc cgccctcgga cgccgcctcc cactccgccg
     4081 agctgacgac gaacgtcgcc tgcgggaact gcgcgatgcc gctcgcgtgg tcgctatgca
     4141 gatgggtcag cacgaccgtc gccacgtcgc cggggtcgaa gccgcgctgg cgcagctggt
     4201 ctgggacggc ctcggcggtg tccattcgga ggtccttgta gatcaggccc ccgagcctgc
     4261 ccatcccctg gcgggggtcg accgcgacgg ccgcgtggaa gccggtgtcg acgagcacga
     4321 ggccggcgcc ggggtgctcg acgagaaagg cttggaccgg cagctcgggc cactcgtcct
     4381 gcgacttccc gacgccgagc gcacgcagcg gcgcgaggcg cccgtcggtg cggtggatca
     4441 gcccgggcgg cgcgaccatc cgcgcgcaca gcagcgggtg cagtcgcacc gtcgccccct
     4501 cgcgcccgcc cggcaacggc agccgggccg gacgcggctc tgccgccgtc gccatgcggg
     4561 caatctaacc cgtgccgata gggtccgggc cgatgggctc ggagatcttc aggcccggcc
     4621 tgctcgaggg tcaggtcgcg ctggtcagcg gcggcggatc cggcctcggc cgtcgcagtg
     4681 cggtcgagct cgcggagctc ggtgcgcagg tcgtcatttg cggacggcgg gccgacccgc
     4741 tcgccgagac tgtcgcgctc tgcgccgacg gccgctgcag cgcccgagtc tgtgacatcc
     4801 gcgaggagga tcaggtcgag gcgctcgtcg acgcggtgct cgccgaccac ggccggatcg
     4861 acctgctcgt caacaacgcc ggtggccagt tcctgacgcc cgccgaggag atcacgccga
     4921 agggctttcg cacggtgatc cggctcaacg tcgaggggac ctggctgatg acccacgccg
     4981 tggccaccag agcgatgatc cccggcggca ccggcggcaa gatcgtcaac gtcacgctgt
     5041 cgccgcacaa cggcctgccc gggatggcgc actcctccgc cgcccgcgcc gcggtcgaga
     5101 acttgacccg cgtgctgtcg atcgagtggt cgcgctacgg gatcaagctc aatgcactcg
     5161 ccgccggcca gttcggcacc gagacgctgc gcacgaagta cccccaggcg atcgtcgacc
     5221 acctcgagca gaccgtcccg ctcggccggc tcggcaccga ggaggagttc gcctgggcga
     5281 tcgcctacct cgcctcgccg gccggcgact tctactccgg cgcggtgatc acgatggacg
     5341 gcgcgcgcga caactggttc ggcacctggc cgccccgctc gatcgccgac gcggggggca
     5401 agccgctctc cgaggagcgg cgcccccgcg atcgcggcta gctcgtcatt ccggtgaact
     5461 ggccaccggt gacgttgagg acctgcccgt ggacgtagtt cgaccacggc gagcagagga
     5521 agaagacgcc gccggcggcc tcctcgggcg tgccgggccg gcccatcggg atcagcatcg
     5581 tcgccatctg acgcagctgc tccgggatgc cgagctggat ctccttgccc tcggcctcga
     5641 tcgtctcggc gttctccttg gaggccgtca ggcgcgtctc gatgaagccg aacgcgaccg
     5701 cgttgacgtt gatcttgaac tggccccact ccttggccag cgtcttggtc agtccgacta
     5761 cgcccgactt gccggacgag tagttcgcct ggccggcgtt gcccatcgtc ccggagactg
     5821 acgagacgtt gacgaccttg cggaagacct cgcggccctc ctcacgctcc tttttcgccg
     5881 gctcgcgcat gtgcggggcg gcggcgcgca ggaccttgaa cgggacgacg ttgtggatgt
     5941 cgagcatcgc ctggaactgc tcgtcggaca tcttgtggat cgggccgtcc caggtgtagc
     6001 cggcgttgtt gacgacgatg tcgagcccgc cgaaggcctc gacggcggtc tcgacgagct
     6061 tgtccggcac gccgtccttg gtcaggtcgc cggcgaaggc gacggtctcg cccgcgatct
     6121 cgcccgcggc ctgctcggcg acgtcgccgt cgaggtcgtt gaccaggacg cgcgcgccct
     6181 gagaggcgag cagctccgcc gtcgcccgtc cgatgccgcg cgccgatccg gtcacgatcg
     6241 ccgactttcc gtcgagaact cccacgtcca ctccctctgt cgttgaagat cgggcggatt
     6301 cttaccgtgt gggcgttcgg tgggagctgt ggaggggtag tcgagacgcc aatggccacc
     6361 acagcgctcg cagtcgtcga catgctcaac ccctacgacc acgaggacgc cgaggtcctc
     6421 gccgagagcg tcgaggacgt cgtcgaaccg atccgcgtcc tgctcgacca cgccggcgac
     6481 gacgacgtcg acgtgatcta cgtcaacgac aactacaacg actggaactc gtcgcaggag
     6541 gagctcgccc gccgggcgct cgacggacgc cggccggacc tcatcgagcc gatcctgccg
     6601 ccggaggagt cctcgttcgt aatcaaggcg cgccacagca tcttctacgg cacgccgctc
     6661 gagcacctgc tgcacgagcg tgagatcgac cggctcgtgc tgtgcggcca ggtgaccgag
     6721 cagtgcatcc tctacagcgc gctcgacgcc tatgtgcgcc agttcgacgt cagcgtcgtc
     6781 ggcgacgcgg tcgcgcacat cgaccgctcg ctcgccgacg ccgcactgcg gatgatggag
     6841 cgcaacatgc gcgcgcaggt cgtggaaagt aggaagttcc actccttact ttgctagcgt
     6901 ggccccatgg ccaccgcgga gactgtcctc aagcccgact tcggatccaa gcgcaagcac
     6961 ttcatctttg aggagcatca cgaccggctg cgcgagtcga tccgctcgtt cgtcgagaag
     7021 gagctcaccc cccacgccga cgagtgggag gagacaacct tccccgactc ggtcatccgg
     7081 cggatgggcg agctcggctt cctcggcctg tcgatgcccg aggagtacgg cggtcaaggg
     7141 ggcgactact tctgcaacct ggtgctcgcc gaggagatgg gccgctcgaa ctccggcggc
     7201 ttgacgatgg gcgtcgccgt tcacaccgac atggcgatgc tgccgatcca cctgttcggc
     7261 agcgaggagc agaagcagca gtggctcgtg ccggcgatca agggcgagaa gatcctctgc
     7321 cttgggatca ccgagccgga tgcgggctcg gacgtcgccg gcatcaagac gcgagcggtg
     7381 cgcgatggcg aggactacgt catcaacggc tcgaagacct acatcaccaa cggccatcgc
     7441 gccgacgtga tcgtgctcgt caccaagacc gatcccgacg ccggctacga cggcttcacg
     7501 ctcttcctgg tcccgatgga cgcgccgggc gtgatccgcg agaagaaact cgagaagctc
     7561 ggcatgcacg cctccgacac ggcgctgctg gccttccagg acgtccgcgt cccggcctcg
     7621 gcgatgctcg gccaggaggg caagggcttc taccacatca tgtgggagct ccagggcgag
     7681 cggctgatcg gcgcggcggg ctgcgtcgcc ggcgcccagc gctgcttcga caagacgctc
     7741 cagtacgccc tcgagcgcga ggcgttcggc cggccgatcg gcaagttcca ggcgatccga
     7801 cacaagttcg ccgagatggc gacgaagatc gaggccgggc ggcagatgac ctacacgacc
     7861 gcctggcgct tcaacaacgg cgagtacccg gtgcgcgaga tctcgatggc gaagctgttc
     7921 accgcccaga tggcctgcga ggtcaccgac gagtgcctgc agatccacgg cggcgctggc
     7981 tacatgcgcg agtacgggat cgagcgggcg tggcgcgaca tgcggctgaa ccggatcggg
     8041 gcggggagcg acgagatcat gctcgacgtg atcgggcggt cgtacgggct ctaggagcgt
     8101 gaggctcgag ccaccgttca ccgacgagca ccaggcgctg cgcgagtcga ttcggcggtt
     8161 cgtggagaag gagctgcggc cgtacgccgc ccagtgggag gacgagcgct ggttcccgaa
     8221 cgacgtgttc gggaagcttg cggcgaacgg gttcctcggc ctgaagtacc ccgagcgcta
     8281 tggcggcgag ggcggcgacc acgtccacga cgcggtgctg atcgaggagc tcggcggctg
     8341 cgggtcgggc ggcgtggcag ctggagtcgt gcgcacataa cgatcgcgac gccgccgatc
     8401 ttcaagttcg ggaccgagga gcagaagcag cgcttcctcg tgccgggaat ccgcggcgag
     8461 aagatcgccg cgctgggaat caccgagccg ggcacgggat cggacgttgc gggcatccgc
     8521 acgcgtgcca agcgtgtcga cggcggctgg gtggtcaacg gctcgaagac gttcatcacc
     8581 ggcggcgtgc gcgccgacgt cgtcgtgacc gccgtcaaga cgagcgacga gggcggccac
     8641 ggcggcctgt cgttcctgct gatcgagaag gggatggagg gcttcaccgt ctcgaagaag
     8701 ctcgagaagc tcggctggca cgcttccgac acggcggagc tggcgttcca ggacgtcttc
     8761 gtgccggagg agaacctgct cggcgatgag aaccgcggct tctacctgat catggccaac
     8821 ttccagtggg agcggatagc gatggcgctc ggcgcggtcg cggcgatgca gcggctgctc
     8881 gacacgtcgg tcgcctacgc gctcgagcgc gaggcgttcg gccggccgat cgggaggttc
     8941 caggcgatcc gccacaaggt cgcccagatg gcggtcaagc ttgaagcgtc gcgcgcgctc
     9001 acctaccacg cgctgcggct ctttcacgac ggccaggacg cgatccgcga ggtgacgatg
     9061 gcgaagctgc tctcacagcg cgctgcctgc gaggtcgccg acgaggcgat ccagattcac
     9121 ggcggcgccg gctacatgcg cgagtacgag atcgagcgcg ccgcccgcga cctccgcctc
     9181 ggcccgatcg gcggcggaac cgacgagatc atgaaggaga tcctcgggcg gcagatgggc
     9241 ttgtagctgc tggccccgtg gaagactcct ccctatgacc gagcgagcct tcgacgcgat
     9301 cgtcctgggc gccgggcccg cgggcgaggt cgtggcggga cgccttgccg acggcgggct
     9361 gtcggtggcg atcgtcgagc cgcatctcgt cggcggcgag tgctccttct acgcatgcat
     9421 gccgtcgaag gcgctgctgc ggccgggcga gctgctagcc gaggcgcggg gcgtgcccgg
     9481 cgtcgcggag gcgattgccg gcgacctcga tgctacggcg acgttggcgc gccgcgacga
     9541 ggtgatccac gacctcgacg actccggcca gctgccgtgg ctggaggaca aggggatcga
     9601 gctgctgcgc ggcgagggcc gtctcgacgg cgagcgacgc gtgcgcgtcg gcgacgagct
     9661 gctgatcgcg cggcgcgcgg tcgtggtcgc gacg
//
LOCUS       DATABV010000103         3524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2681434, whole genome shotgun sequence.
ACCESSION   DATABV010000103 DATABV010000000
VERSION     DATABV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2681434"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VHF88_04355"
     CDS             <1..339
                     /locus_tag="VHF88_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161036.1"
                     /translation="EGEPFITPDRKVALPPRHELATSMEALIHHFKLVTEGFRVPSGE
                     AYCAIESPRGELGCYILADGSAKPARVHVRDPSFVNLQALPEMSEGGLLADMIANIAM
                     LDPILGGIDR"
     gene            336..1103
                     /locus_tag="VHF88_04360"
     CDS             336..1103
                     /locus_tag="VHF88_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2161037.1"
                     /translation="MSPHAPEPGATGRVDRRLTPLRWDEAADLDKPPATIPEATETEV
                     PAELRERIEALMSRYPDRRSAALPALDAAQRVHGWCSPEAIDQVAAVMRVTPAYLSSV
                     ATFYDMLRTEPLGRRYVYVCTSVACHLRNAKAVHDAIADEAREQGLEDCDIRQFECLG
                     ACDMAPMASIDGRYVGPLDVSDAAEVVAALKEDREVFPGRGLTDRDFELPWAQGEVTG
                     GAQTDRGAEGGPGSSGARSGEHGTDGSPPTPPDTNRA"
     gene            1100..2446
                     /gene="nuoF"
                     /locus_tag="VHF88_04365"
     CDS             1100..2446
                     /gene="nuoF"
                     /locus_tag="VHF88_04365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX2161038.1"
                     /translation="MTDTLETKVLTANLDVANLDLIHVYESTGGYRSARKAALEMTQD
                     EVLEQLESSGLRGRGGAGFSMGKKASFIPKGSMDKFLCCNADESEPGTFKDRLLMQRN
                     PHLLVEGIIIGAIAAGANRAFIFIRGEYSAQADILDRAVEQAYDAGYLGDGIFGSDIQ
                     VELVVHRGEGAYICGEESALLDALEGKRGNPRLKPPFPANQGLYQGPTLINNVETLCN
                     TPIIIEKGAEWYRQFGVGNSTGTKMVSVSGNVQRPGNYEIELGITAREIVYGLAGGPL
                     PGREIKCWFPGGSSAPVLLPEDLDRPYTFESMAEAGSMLGSGAIIVVDDSVPIVGIAL
                     RLAEFYRHESCGKCVPCREGTNWTVKMLERLDRGEATPMDIEIISSVQDNIIGNCLCV
                     LGDSMAMPIGSMIKHFRPEFEAHIEAARERADRAAAVGEFSAVELAASPHSGPGVG"
     gene            2446..>3524
                     /locus_tag="VHF88_04370"
     CDS             2446..>3524
                     /locus_tag="VHF88_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2161039.1"
                     /translation="MARPEPRKIVFELDGREVEAAEGTLLVDAARNGDVEIPYFCYEP
                     KLGPPVGACRMCLVEIEGIPKLQTSCSTPVKDGMVVHTTTDRVKEAQNAVVEFLLVNH
                     PLDCPVCDKGGECPLQDISYGWGPGRSRMIEPKRHFKKPLEVSPLVAIDRERCILCYR
                     CVRFSQEVAEDYQLVFLERGDHTYVGTHDGAPYVAPFSGNITELCPVGALTPTTYRFR
                     ARPWDVEEAGSVCIGCASQCNVRYTVRDDDKVLRVLARDNDEVDDGWVCDKGRFGYES
                     FHSPERVTAPMVRDGGVLRAVSWERALDEAAKALARGGERTAALAGGAVTNEEGLLLQ
                     HLLRAGLGSPHVDARSGGAIDAAQAR"
BASE COUNT          543 a         1259 c         1230 g          492 t
ORIGIN      
        1 gagggcgagc cgttcatcac gcccgaccgc aaggtcgcgc tgccgccgcg ccatgagctc
       61 gcgacctcga tggaggcgct gatccaccac ttcaagctcg tcacagaggg cttccgcgtg
      121 ccgtccggcg aggcctactg cgcgatcgag tccccgcgcg gcgagctcgg ctgctacatc
      181 ctcgccgacg gctccgcgaa gccggcgcgc gtccacgtgc gcgaccccag cttcgtcaac
      241 ctgcaggcgc tgccggagat gagcgagggc ggcctgctcg ccgacatgat cgccaacatc
      301 gcgatgcttg acccgatcct cggggggatc gaccggtgag cccgcacgcc cccgagccgg
      361 gcgcgaccgg gcgcgtcgac cgtcgcctca cgcccctgcg ctgggacgag gcggccgacc
      421 tcgacaagcc gccggcgacg atccccgaag cgaccgagac cgaggtcccg gccgagctgc
      481 gcgagcggat cgaggcgctg atgtcgcgct atccggatcg ccgctcggcg gcgctgccgg
      541 cgctcgacgc cgcccagcgt gtccacggct ggtgctcgcc ggaggcgatc gaccaggtcg
      601 ccgccgtgat gcgcgtgaca cccgcctatc tctcctcggt cgcgaccttc tacgacatgc
      661 tgcgcaccga gccgctcggc cgccgctacg tctacgtctg cacgagcgtc gcctgccacc
      721 tgcgcaacgc gaaggcggtc cacgacgcga tcgccgacga ggcgcgcgag caggggctcg
      781 aggactgcga catccgccag ttcgagtgcc tcggcgcctg cgacatggca ccgatggcct
      841 cgatcgacgg ccgctacgtg ggaccgctgg acgtgtcaga cgcggccgag gtcgtcgcgg
      901 cgctgaagga ggaccgcgag gtcttccccg gccgcggcct gaccgaccgc gacttcgagc
      961 tgccgtgggc ccaaggagaa gttacgggcg gagcgcagac ggatcgcgga gctgagggag
     1021 gtcccggatc tagcggggcg cgatccggcg agcacggcac cgatggatct ccgccgaccc
     1081 ctcccgacac gaaccgcgca tgaccgacac gctcgagacc aaggtcctca ccgccaacct
     1141 cgacgtggcg aacctcgacc tgatccacgt ctacgagtcg accggcggct accgctcggc
     1201 gcgcaaggcg gcgctcgaga tgacccagga cgaggtgctc gagcagctcg agtcatccgg
     1261 cctgcgcggc cgcggcggcg ccggcttttc gatgggcaag aaggcgagct tcatccccaa
     1321 ggggtcgatg gacaagttcc tctgctgcaa cgccgacgag tccgagccgg gcacgtttaa
     1381 ggaccggctg ctgatgcagc gcaacccgca tctgctggtc gaggggatca tcatcggcgc
     1441 gatcgccgcc ggcgccaacc gcgccttcat cttcatccgc ggcgagtact cggcccaggc
     1501 cgacatcctc gaccgcgcgg tcgagcaggc ctacgacgcc ggctacctcg gcgacgggat
     1561 cttcgggtcg gacatccagg tcgagctggt cgtccaccgc ggcgagggcg cctacatctg
     1621 cggcgaggag tccgcgctgc tcgacgcgct cgagggcaag cgcggcaacc cgcgcctcaa
     1681 gccgccgttc ccggcgaacc agggcctcta ccagggaccg acgctgatta acaacgtcga
     1741 gacgctctgc aacaccccga tcatcatcga gaagggcgcc gagtggtacc gccagttcgg
     1801 cgtcggcaac tcgaccggca cgaagatggt gtcggtctct ggcaacgtcc agcggccggg
     1861 caactacgag atcgagctcg ggatcaccgc gcgcgagatc gtctacggac tggccggcgg
     1921 tccactgccg gggcgtgaga tcaagtgctg gttccccggc gggtcgtcgg ccccggtgct
     1981 gctgcccgag gacctcgacc ggccctacac gttcgagtcg atggccgagg cgggctcgat
     2041 gctgggctcg ggcgcgatca tcgtcgtcga cgactcggtg ccgatcgtcg gcatcgcgtt
     2101 gcggctggcc gagttctacc gccacgagtc gtgcggcaag tgcgtgccgt gccgcgaggg
     2161 gacgaactgg acggtgaaga tgctcgagcg gctcgatcgc ggcgaggcga cgccgatgga
     2221 catcgagatc atctcctcgg tccaggacaa catcatcggc aactgcctct gcgtgctcgg
     2281 cgactcgatg gcgatgccga tcgggtcgat gatcaagcac ttccggccgg agttcgaggc
     2341 ccatatcgag gcggcgcgcg agcgcgccga ccgtgccgcc gccgtcggcg agttctcggc
     2401 ggtcgagctt gccgccagcc cgcactcggg accgggggtc ggctgatggc gcgccccgag
     2461 ccgcgcaaga tcgtcttcga gctcgacggc cgcgaggtcg aggcggcaga gggcacgctg
     2521 ctcgtcgacg ccgcccgcaa cggcgacgtt gagatcccct acttctgcta cgagccgaag
     2581 ctcggaccgc cggtcggcgc ctgccggatg tgcctggtcg agatcgaggg catcccgaag
     2641 ctgcagacct cgtgctcgac gccggtcaag gacggcatgg tcgtccacac cacgaccgac
     2701 cgcgtcaagg aggcgcagaa cgcggtcgtc gagttcctgc tcgtcaacca cccgctcgac
     2761 tgcccggtct gtgacaaggg cggcgagtgc ccgctgcagg acatctccta cggctggggc
     2821 cccggccgct cgcggatgat cgagccgaag cgccacttca agaagccgct cgaggtctcg
     2881 ccgctggtcg cgatcgaccg cgagcgctgc atcctctgct accgctgcgt gcgcttctcg
     2941 caggaggtcg ccgaggacta ccagctcgtc tttctcgagc gcggcgacca cacctatgtc
     3001 gggacgcacg acggcgcgcc gtatgtcgcg ccgttcagcg gcaacatcac cgagctctgc
     3061 ccggtcggcg cgctgacgcc gacgacctac cgcttccgcg cccgcccatg ggacgtcgag
     3121 gaggccggct cggtctgcat cggctgcgcc agccagtgca acgtccgcta cacggtccgc
     3181 gacgacgaca aggtcctgcg cgtgctggcg cgcgacaacg acgaggtcga cgacggctgg
     3241 gtctgcgaca agggccgctt cggctacgag tcgttccact cgccggagcg cgtcaccgca
     3301 ccgatggtcc gcgacggcgg cgtgctgcgc gcggtgtcat gggagcgggc gctggacgag
     3361 gcggcgaagg cgctggcccg cggcggcgag cgcaccgccg cgctcgccgg cggcgcggtc
     3421 accaacgagg aggggctcct gcttcagcac ctgctgcgcg cggggctggg atcgccgcac
     3481 gtcgacgcgc ggtcgggcgg cgcgatcgac gcggcgcagg cgcg
//
LOCUS       DATABV010000104         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_111734, whole genome shotgun sequence.
ACCESSION   DATABV010000104 DATABV010000000
VERSION     DATABV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111734"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1683
                     /gene="cimA"
                     /locus_tag="VHF88_04375"
     CDS             37..1683
                     /gene="cimA"
                     /locus_tag="VHF88_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX2161040.1"
                     /translation="MAEQVLLYDTTLRDGMQGEGMSLSADEKVRVAHALDELGVHLIE
                     AGFPSSNPKEQALFELLAGESFENAQIAAFGMTRRKDSAAADDPALRVLADGFAPVCT
                     LVGKTWSLHLEKVTRVDPEENLRMIADSVAFLRGEGKRVIYDAEHFFDAYADDPAYAL
                     RCLRAAAEAGAENLTLCDTNGATLPGAISEATAAVVQELGGAAQVGIHTHDDAGCGVA
                     NSLVAVERGARLVQGTVNGYGERCGNANLLTILANLQLKLGYDCVEPGRLERLTETAH
                     LIDELCNVSPNPNQPYVGANAFAHKGGMHVAGVNRDARTFEHVDPRQVGADRRLLISE
                     LSGKGTVEARAAKTGVDLGPDGAARVADRVKELEHRGYHLEAADGSFDLLIRKQAGDY
                     EPLFTLESWRVIVEKRNDLGAQTPSASAQHGAVETEATIKIWVDGERYIRTAEGNGPV
                     NALDNALRDAICERHPHLRDIELVNYKVRILDESKGTGAATRVLLDAGDGSETWGTIG
                     VSENIIEASWEALVDSLEAGMLPGRAEHVRKAARSEVPSS"
     gene            1680..>1918
                     /locus_tag="VHF88_04380"
     CDS             1680..>1918
                     /locus_tag="VHF88_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2161041.1"
                     /translation="MTEPFIPVARPVIGQREKELVLDVLDSRHLSLGPRLAEFEQAFA
                     DRLGVEHASAVSSGTAGLHLALRAAGVEAGDEVVTT"
BASE COUNT          318 a          680 c          659 g          261 t
ORIGIN      
        1 tctctcgaag tcgacatccg aaaggcgttc acgcagatgg cagagcaggt acttctctac
       61 gacaccaccc tccgcgacgg gatgcagggg gaggggatgt cgctgtccgc cgacgagaag
      121 gttcgcgtcg cccacgcact cgacgagctt ggggtgcatc tgatcgaggc cggcttcccg
      181 agctcgaacc cgaaggagca ggcgctcttc gagctgctcg cgggcgagag cttcgaaaat
      241 gcgcagatcg ccgccttcgg catgacgcgc cgcaaggaca gcgccgccgc cgacgatccc
      301 gcgctgcgcg tgctcgccga cggcttcgcg ccggtctgca cgctggtcgg caagacgtgg
      361 tcgctgcacc tcgagaaggt gacgcgcgtc gatccggagg agaacctgcg gatgatcgcc
      421 gactccgtgg cgtttctgcg cggcgagggc aagcgcgtca tctacgacgc tgagcacttc
      481 ttcgacgcgt acgccgacga cccggcctac gcgctgcgct gcctgcgcgc ggccgccgag
      541 gcaggcgccg agaacctaac gctctgcgac accaacggag cgacgctgcc gggggcgatc
      601 tcggaggcca ccgccgccgt cgtccaggag ctgggcggag cggcacaggt cggcatccac
      661 acccacgacg acgcgggctg cggggtcgcc aactcgctgg tcgccgtcga acgcggcgcg
      721 cggctggtgc aggggacggt caacggctac ggcgagcgct gcggcaacgc caacctgctg
      781 acgatcctcg ccaacctgca gctcaagctc ggctacgact gcgtggagcc cggccggctc
      841 gagcggctga ccgagaccgc gcacctgatc gacgagctct gcaacgtctc gccgaacccg
      901 aaccagccct acgtcggcgc caacgccttc gcccacaagg gcggcatgca cgtcgccggc
      961 gtcaatcgcg acgcgcgcac cttcgagcac gtcgatccgc gtcaggtcgg cgctgaccgg
     1021 cgactgctga tctccgagct gagcggcaag gggaccgtcg aggcgcgcgc cgcgaagacc
     1081 ggcgtcgacc tcggaccgga cggcgccgcg cgcgtggccg accgcgtcaa ggagctcgag
     1141 caccgcggct accacctcga ggccgccgac gggtcgttcg acctgctaat ccgcaagcag
     1201 gccggcgact atgagccgct gttcacgctc gagtcctggc gcgtgatcgt cgagaagcgg
     1261 aatgacctcg gtgcgcagac tccgtctgct tccgcccagc acggcgcggt cgagacggag
     1321 gcgacgatca agatctgggt tgacggcgag cgctacatcc gcaccgccga gggcaacgga
     1381 ccggtcaacg cgctcgacaa cgcgctacgc gacgcgatct gcgagcgcca tccgcacctg
     1441 cgcgacatcg agctcgtcaa ctacaaggtg cggatcctcg acgagagcaa gggcaccggc
     1501 gccgcgacgc gcgtcctgct cgacgccggc gacggcagcg agacctgggg cacgatcggc
     1561 gtctcggaga acatcatcga ggccagctgg gaggcgctcg tcgactcctt ggaggccggc
     1621 atgctccccg gccgcgctga acatgttcgc aaggctgccc ggagcgaggt cccgtcctct
     1681 tgacggagcc gttcataccg gtcgccaggc cggtgatcgg ccagcgcgag aaggagctcg
     1741 tgctcgacgt cctcgactct cgccacctct cgctcggacc acgcctggcc gagttcgagc
     1801 aggcgttcgc cgatcgcctc ggcgtcgagc acgcttcggc ggtctcgagc ggcacggccg
     1861 gtctgcacct cgcgctgcgc gcggcgggtg tagaggccgg cgacgaggtc gtcaccac
//
LOCUS       DATABV010000105        19725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1959377, whole genome shotgun sequence.
ACCESSION   DATABV010000105 DATABV010000000
VERSION     DATABV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 19725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19725
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959377"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VHF88_04385"
     CDS             <1..789
                     /locus_tag="VHF88_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161042.1"
                     /translation="FAAVMITCNGIIGISILAAALRDRIAVFNPEGSATAFATVITLA
                     TLSLVLPTFTTSESGPEFSASQLAFAAVASLALYVLFLIVQTVRHRSHFMPEGEDSGV
                     TELHGSPPTTAAALRSLGLLLVSLVAVVGLAKTSSGAIESAVASVGAPQSAVGVVIAL
                     LVLMPETLAATRNARRGRVQISFNLGYGSAMASIGLTIPVIAVASIWLEGPLVLGLGS
                     TQIVLLVLTALVSSLTLLPGRATLQEGGVHLILLAAFLFLAFDP"
     gene            complement(873..2717)
                     /locus_tag="VHF88_04390"
     CDS             complement(873..2717)
                     /locus_tag="VHF88_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2161043.1"
                     /translation="MDNELAVLGAALLLAGLFARFGRRLGLPTIPFFIVAGIVFGPNT
                     PGLVVLDDPEGIHLLATLGLVLLLFHLGIEFSIDDLMSGGRRLLWAGGSYIVLNFGAG
                     LVLGWALGWGTREIFVIAGMIGTSSTAIVTKLLLDLRRVSNPETGMILGIIVVEDVFI
                     AFYLALLQPILGDQQGAYEILESIAIAFAFLTGLFALARWGARLVRPLISADDAELTI
                     ILSVGFGVFVAGFAHIAGASDAVGALLAGMVVAGTGIGPRVERLLVPLRDTFAAIFFF
                     WFGLTIAPSDMGAVASAVAVAVAVTLLFNVVAGVVAARVYGYGRLAASNTALMLVSRG
                     EFELILASLAVAAGLDSRVAPFAALYVLVLSILSPLLSARSHVLARWLPRRLFGPDSD
                     RDAAEAPPPSEPIIEVGALRRLGAETVAHLVRPNHAIVDMYVRDLGLPRDALVSVIVR
                     SGEAIAPRGSTRIRAGNRLHVLVRREAARELDGLQERWRDGPIGAPPRPRRQTRGSSP
                     VWRTCGGESPAWTRTSAPGCSRSSERSRSTCSKGRLARRWPRDPARAPAGQRPRAAGE
                     LDDVVRALYPAALIQTSAVGVACGERREGAGDGPEGLDDPGRIVGCRS"
     gene            2957..4303
                     /locus_tag="VHF88_04395"
     CDS             2957..4303
                     /locus_tag="VHF88_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2161044.1"
                     /translation="MIEVLFLVVSAVLVVACGAFVAAEFSLVTVDRSAVERAAERGDA
                     GAAGVLKALSSLSTQLSGAQLGITLTNLLIGFVAEPTIADLIDGPLESAGVPAAIVTG
                     VALVIALSAATTATMVVGELVPKNLAIARPLQTARAVQGFMRGFTRVNAPLLGVFNGA
                     ANGVLRWFGIEPQEELASARSPDELSSLIGRSAAQGTLEEETAALLRRSLAFAERQAQ
                     DVMTPRTRLHTIGATEPIQAVIDMARATGRSRFPVLGEGPDVVVGVVHVKHAVGVPPE
                     RREIARVADAMVAPVVVPSTVELDPLLGRPLWRSLQMAVVVDEFGGIDGLVTLEDIVE
                     EIVGEVLDEHDRRDPAIRRDPAGGWVLSGLLRPDEASDAVGAALPESDDYETLGGLIA
                     TELGRIAHVEDRVSLEVRDAEGLRREAQLTVARMDGWRVDRVRLRLGEALPDEEHE"
     gene            4300..5343
                     /locus_tag="VHF88_04400"
     CDS             4300..5343
                     /locus_tag="VHF88_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2161045.1"
                     /translation="MNDWLALGLGVFLLALNAFFVGAEFALISARRSAIEPKAQEGSR
                     RAKVTLRAMEQISLMLAGAQLGITICSLLLLAVVEPAVAHLLEGPFGALGVSDALRHP
                     VAFAIALAAVGVSHVVLGEMVPKNIALAKPDRAALALAPPLLLIVRLLRPAIAALNAI
                     ANGMLRLVGVQPQDEVTSSFTREEVAGFVDESRREGLLHPSEERTLIGALQFGERTAT
                     NVLLPLDDVETLPIAVTPHDVERAAARTGFSRFPVQDEYGAPIGYLHLKDALETKDVH
                     RNRPIARRWVRPLPAIPATDPLLSVLATMQRSGAHLAQAVDGDRGLIGIVALEDVLEE
                     LVGEIRDGTRRAA"
     gene            complement(5436..6662)
                     /locus_tag="VHF88_04405"
     CDS             complement(5436..6662)
                     /locus_tag="VHF88_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX2161046.1"
                     /translation="MALDPGWTVAELRELAALTGDERGAQRLAWTPTWARAREWLRVR
                     LAELPVEVEVDPAGNLWATLPGASPRAVVVGSHLDSVPDGGWLDGTLGVLAALEVLRR
                     VAGEGQPPLTLRLVDWADEDGARFGRSLIGSEAAAGLLDGEELAALADRDGVGVREAL
                     AEHGVELARMGEAHAQLADVVAYLELHIEQGVELERRDLPVGVVSGVLGVVRHAVRFE
                     GETAHAGATALELRRDALLSAARLALEVREIARRQGGRGTTGDVAAAPGIVTAVAGEA
                     TLLVDQRHETPELLADVERRAREAADRIAAEERTTVAWETLLRTAPVPFDPLLVSIAD
                     DVVTELAGEALPISSGALHDAAAVARAGVPAAMLFARSIGGRSHTREEDTRPEDLEAA
                     VRALDALVERVLTALR"
     gene            complement(6669..8021)
                     /locus_tag="VHF88_04410"
     CDS             complement(6669..8021)
                     /locus_tag="VHF88_04410"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2161047.1"
                     /translation="MEGATVASGEQAKQWDRDHVLYPWAIQAQVEPLVVTGAEGRRFW
                     DSDGNSYLDFASQLVNVSIGHQHPRVVAAIKEQADRLCTIGPPLANEPRSRLARLLAE
                     VTPGDLSMAFFTNGGAEANEYAIRLARRYTGRQKVVARYRSYHGGTAGAIALTGEPRR
                     WPTDPGIPGVVRMLDPYTYRCPAGHPDPCPVCSGGPHLEEILQYEGPENVAAVVLETI
                     TGTNGILVPPDGYLRSIREVCDRHGILLIADEVMAGFGRSGEWFAVDHWDVVPDILTV
                     AKGINSGYVPLGAMVVREPVAAWARESFFPGGLTYSGHPLACASAVASIEAFRDEGIV
                     EHAARIGGVIGSALRELQDRHPSIGEVRGLGCFWGLELVRDRATREMLVPFNASGNDV
                     KPLARMLAAARERGLFMWGHWNVLAVTPPLTITDEEVEEGVAILDEVLSIADELYTGG
                     "
     gene            complement(8023..8388)
                     /locus_tag="VHF88_04415"
     CDS             complement(8023..8388)
                     /locus_tag="VHF88_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2161048.1"
                     /translation="MHRVTLGRPIGEQSDWEMFRGEVHYQPIANEQNAELFRSAHISF
                     RPGARTQWHHHGCDQMLIVTGGHGTVASEDEEIPVRTGDVVIVPAGTRHWHGATAESD
                     LSHISVLTPADEVIHEDQD"
     gene            complement(8405..9436)
                     /locus_tag="VHF88_04420"
     CDS             complement(8405..9436)
                     /locus_tag="VHF88_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX2161049.1"
                     /translation="MTQSSNGVPRMRPAEIVTRSHWQDPNEVRVANWIREWDGAEEID
                     AAIVGVPLARAAIKQVGANYAPNAVRQTGFFFTTYSIDFDFDLRDMVVRDLGDVEVPL
                     IDVEQAHANVAEAATAIFAHRPAYVPVFVGGDHSIMRPIVQGLAAGRPDVERIGIVHF
                     DAHLDAQVLDFCGPHNGTPLRGLLGDGIGVEARNIAQIGISGFVNGGYYRDYVREQGT
                     TVFSARDVARGKIEEIVAEAHAIAADGTDAVYVTFDIDCMELAYAPGTGASISGGLNA
                     WQAIEALHALGLQEDVVALEVSEIDPTRDFGDMTSRLGMKLILSFLAGVHQRAARPTT
                     AATVASTTP"
     gene            complement(9474..10256)
                     /locus_tag="VHF88_04425"
     CDS             complement(9474..10256)
                     /locus_tag="VHF88_04425"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161050.1"
                     /translation="MSVVEIDGVSMGFDTRVGRIEALEDVSLTVADGEFACLLGPSGC
                     GKSTLLNVIAGLANPSSGSVAVDGEEVHEPGRDRGMVAQTYTLFPWLKVRANIEFGPK
                     LAGVPRAERARISERLLDRMGLTEFADAYPRELSGGMQQRVAIARAFANEPRVLLMDE
                     PFGALDALTRTLAQDFLTEVWEADRRTIVFVTHDIAEALMVGDVLYVMGTRPGRICER
                     IEVEFPRPRTREIEEDPRFGALRHRILNLIRGEVVPEGEVVA"
     gene            complement(10253..11119)
                     /locus_tag="VHF88_04430"
     CDS             complement(10253..11119)
                     /locus_tag="VHF88_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2161051.1"
                     /translation="MAAGAGALPQPRRAPRRRRGGGRGRQSYLTPRAPIPRWMRTALA
                     FGGGLTVIALWSALSYGGVYDEFFLPTPTTVASAWWDMITKQGFASDIGASAGRIGLG
                     FLLAAVIAVPLGIAIGSFRVVQSYFEPTLAAIRYMPASAFIPLLILWLGIGEQEKVAV
                     IFLGVFFSLTLLIADVSANVPQSLLNISYTLGASRRKVFTRVLLPACWPGVLDNLRIG
                     MGWAWTYLIVAELVAASSGIGHVILASSRTLSTDQVIAGILTIGALGLITDGLFRWAY
                     RMLFPYAERTAR"
     gene            complement(11146..12159)
                     /locus_tag="VHF88_04435"
     CDS             complement(11146..12159)
                     /locus_tag="VHF88_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2161052.1"
                     /translation="MTGKLFATTLVAILMALAVGCGSDDDGGGGGGGSGGEGGMAPVR
                     LALSSWPGFGALYIADEQGYYRDAGLEVDLDLVEDQTNRVQAMQAGEKDGAGMTADTW
                     VRWAAQGVQAKQVLATDESFGGDGIVARDDIAEPSDLRGVRVGVADGSTAEFLLGYVL
                     REAGLTLDDVDKADMSPADAGAAFAGGSVPAAATWQPWLSRAEESPNGHVLADTKEYP
                     VVVTTVGLSSDFIEENPESVQALVEGTDRAISFLRSNPKEAAAIIARATDQSPDDVTA
                     ILGDLRLYGAKENQEFFGTEDQPGRIYELWEAAGEIWQQAGDIDSTPSAEDGIDPSFV
                     NAQ"
     gene            complement(12156..13748)
                     /locus_tag="VHF88_04440"
     CDS             complement(12156..13748)
                     /locus_tag="VHF88_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2161053.1"
                     /translation="MVSGHESPPSGSQSPEAARSDGKALDAYEERLGQGVRDFLGAGP
                     LPLSIGGREIAVDRETIPVEDPAQGAVVAEVPAAGRREVDQAVEAARAAMSGPWRRMG
                     AHQRSMTLHALADLVEEHGETFAQLESLDSGKPVAAARDYDVAKTVEFFRYFAGWPTK
                     IEGSTIPGPGEHRLIYTLRQPVGVVAQILPWNFPLMIAAWKLAPALAAGCMAVVKPAE
                     QTPLSVLYLAQLLREADLLPPGVVNVVTGYGGEAGAALAGHDGVAKVSFTGSPEVARE
                     IVRAAAGNLKRVTLELGGKSPNIVLPDMDPAEIAPEAAAAIFLNQGENCIAGSRLFVH
                     RSIFDDVVAGVRDSADEVRLGPGLDETTTMGPLISAQHRDRVLEYVGAGAEAGGDVHR
                     APAGDRPPGGYFVEPTVITGVSDDARVAREEIFGPVLVALPFESLEEVAARANSTEYG
                     LAAGIWTHDVAAAHDLARMLEAGTVWINTYNETDPAVPFGGFKQSGWGREHGGEVLDQ
                     HLETKSVWVNLERRSRGQGELR"
     gene            13825..14445
                     /locus_tag="VHF88_04445"
     CDS             13825..14445
                     /locus_tag="VHF88_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2161054.1"
                     /translation="MRDQVYERLRSAILGGEIPAGERVSPPELAERLGVSTTPVREAL
                     RSLEEEGLIETSPRRWTRVASPSPELAAETYPVIATLEELAVASIETVTPDLLEELRA
                     ANRDFERAAADGDVFACMTANDAFHGALLRANGNGTLERTLRDLKTRIRLLDSQYFQS
                     GTETSIREHEQVIDALERGDIEQAAGVIRGQWVRWLPAWGVSPAEH"
     gene            complement(14442..15557)
                     /gene="ald"
                     /locus_tag="VHF88_04450"
     CDS             complement(14442..15557)
                     /gene="ald"
                     /locus_tag="VHF88_04450"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX2161055.1"
                     /translation="MLVGVAKETKTAERRVALTPGGARELVASGHRVIVERGAGAGSR
                     YADADYEAAGARIAGTDEVWADSELLLKVKEPTPSEHRRLHPGLTLFTFLHLAADPDL
                     TQALVDSGTTAIAYETIEDEHGRLPLLAPMSEVAGRLATQAGAYFMQSQLGGSGVLIG
                     GVPGVDPARVLVVGGGAVGTQAARVAVGMGAQVTVLERSPARIRELEAYFDTRARVLM
                     SDAGSIAEQVADADVVIGAVLIPGARAPRLISRAMLSDMRPGTVVVDVAIDQGGCVET
                     AVPTSHADPVYEVEGVVHYCVSNMPGAVPVTSTRALTNATRPYVQRLADLGVEAAIDG
                     DPGLAKGLNVAGGQIVCEPVAEAFAGARRAERTAVGA"
     gene            complement(15558..16940)
                     /locus_tag="VHF88_04455"
     CDS             complement(15558..16940)
                     /locus_tag="VHF88_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2161056.1"
                     /translation="MSDRRLLLHFSRNGAFADPSDLLVLERGEGPYVFDTEGRRYADA
                     LSSLFCCQIGYSHGDEMAEVAGDQLRRLAFNTNWATAHPPALALADRLVELAPEGIGK
                     VLFTSGGSESVEAAWKIVRQYHVAMGEPQRTKAIARRTAYHGVTLGALALTGVEAFKQ
                     PFGEPAIPTRHVSNTNSFRDERSEQELCRDLLAELEATIEEEGADTIAMFIAEPIQNA
                     GGCFTPPDGYWPGVREICDRHGILLVADEVISGFGRLGEWFAVERFGGRPDLITVAKG
                     LTSAYAAMGAVLCSDRVAAPLYDDKRVLLHGITFGGHPASAAIALRNIEIFEREGVLA
                     NVRRLEGYLERALRERVGCLPIVGDVRGAGFFWAMELVKEGGERFDAPERERLLRGFM
                     PRRLLEEGLIARADDRGDSVLQIAPPLIADEAFLDEVVDGVAAVLAGAGDEMGLTASL
                     ASGGVGGEAA"
     gene            complement(16937..18445)
                     /locus_tag="VHF88_04460"
     CDS             complement(16937..18445)
                     /locus_tag="VHF88_04460"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2161057.1"
                     /translation="MPNTSSIAPAADSVTRSDDAAAPRLGNFVGGRWVELPEREAIDC
                     LDPASGELIATVPLSGADDVDRAVRAARDAFPGWRATSPLRRARALFGLRDLLTRHAD
                     ELAELVTRDMGKTTADARAEVGRGIESVEAACAAPHMLKGENLEGVGTGVDVELVRQP
                     VGVVAGITPFNFPAMIPLWFLPYAVVCGNTVVMKPSEADPLTSVRMFELIADAELFPP
                     GVVNLVNGAHEAVDGLLDHPGVDAISFVGSAKTARYIYERAATRGKRVQALGGAKNAM
                     VVMPDADPELMLDGVLSSAFGAAGQRCLAGSIAVLVGTPEEQDRARERIVAGAQALRT
                     GPGADPQTDVCPLVSAQARERLEGEIEALAAEAEIVLDGRGASGAGGTELRPTIVDRA
                     PLDSRAVTEELFGPVLTLLRARDLDDAIEIVNSSRYGNASVIFTSSGQAARSYRYSVE
                     AGMVGVNVGVAAPVAWFPFAGWKDSFDGDLHANGPDAFEFYTRKKVVTSRWA"
     gene            18541..>19725
                     /locus_tag="VHF88_04465"
     CDS             18541..>19725
                     /locus_tag="VHF88_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX2161058.1"
                     /translation="MDALACVHLTVDATATEPSLVTVATVLATPELQRGVPEVVAGAD
                     GLGRPVRWVHAAEKPDIAALLKGGELLLTTGLGMQRAAADQRRFVRELSARSVAGVVI
                     ELGSRFDSVPEAIRDEAERATLPLIALHREVRFVDITEAIHREIVNRQFELMRRGDEV
                     HRRFRNLLLDGAGVPEVLDALAEVVDNPVMLERAGHGLLYHSAGRSGEPAALAGWASV
                     AGGLGGAPQFVEQAIPVAGDDAGGRVVAIGIDSPLDESDRVAVERAGELIALALLRDR
                     EEDRLVGRERGNFLLSLLRGEAIDERAAGERARALGFPHAGRMLPIIAARSVSASAAA
                     GSEARVWSRLWSDVLGRLADRRLAVLAGTREHERELLCVVGIPGRADRTELMDRVAAI
                     AHE"
BASE COUNT         2747 a         7116 c         7068 g         2794 t
ORIGIN      
        1 ttcgccgccg tgatgatcac ctgcaacggc atcatcggca tctcgatcct cgccgcggct
       61 ctgcgcgacc ggatcgccgt cttcaacccc gagggctcgg ccacggcctt cgcgacggtc
      121 atcaccctgg cgacgctcag cctcgtcctg ccgaccttca ccacgagcga gtcgggcccc
      181 gagttctccg cctcgcagct cgccttcgcc gcggtcgcgt cgctcgcgct ctacgtcctc
      241 ttcctgatcg ttcagacggt tcggcaccgc agccacttca tgcctgaggg cgaggacagt
      301 ggcgtgaccg agctgcacgg gtcgccgcca acgacggcgg ccgccctgcg cagcctcggg
      361 ctgctgctcg tctcgctggt cgcggtcgtc ggcctggcca agaccagctc cggggcgatc
      421 gagagcgcag tcgcgtcggt cggcgcaccg cagtcggctg tcggcgtggt gatcgccctg
      481 ctcgtcctga tgccagagac gctggccgca acccgcaacg cccgtcgtgg ccgcgtgcag
      541 atcagcttca acttgggcta cggctcggcg atggccagca tcggactgac gatcccggtg
      601 atcgccgtgg cgtcgatctg gctcgagggc ccgctggtgc tcggtctcgg ctcgacgcag
      661 atcgtcctgc tggtcctcac ggcgctggtc agttcgctga ccctgcttcc cggccgcgcg
      721 accctgcagg agggaggcgt gcacctcatt ctgctcgccg cgttcctatt cctggccttc
      781 gacccctgag ccgaggtcgg atacgtataa ccacccattg cggcccctct cgtcgcgcgg
      841 cagcgtgagg agcgtgagcg tcgtcgtctg ggtcatgatc ggcatccgac aatccgcccg
      901 gggtcgtcca agccctctgg gccatccccg gcaccctcgc ggcgctcgcc gcaagctacg
      961 cctacggcgc tcgtctggat cagcgccgcg ggatagagcg cgcgcacaac atcatctagc
     1021 tctccggcgg ccctaggtcg ctgtccagca ggggctcgtg ctgggtccct aggccagcgc
     1081 cgggctagcc ggcccttcga acacgtcgat cgcgagcgct ccgacgaccg actgcagcca
     1141 ggcgcgctcg tccgggtcca cgctggcgac tcgccgccgc acgtacgcca gaccggcgac
     1201 gagccgcgcg tctggcgacg gggcctgggc ggggcgccga tcggaccgtc gcgccagcgc
     1261 tcctgcagtc cgtcgagctc cctggcggcc tcacgccgga cgaggacgtg caggcgattg
     1321 cccgcccgga tgcgggtcga cccccgcgga gcgatggctt cgccgctgcg gacgatcacg
     1381 ctgaccagcg cgtcgcgcgg caggccgaga tcacgcacgt acatgtcgac gatcgcgtgg
     1441 ttggggcgaa ccaggtgcgc caccgtctcg gcgccgaggc ggcggagcgc tccgacctcg
     1501 atgatcggct cggaaggcgg gggcgcctcg gcggcgtcgc ggtccgaatc gggtccgaag
     1561 agccggcgag gcagccagcg agcgagcaca tgcgaccgcg ctgagagaag cggggacaaa
     1621 atcgacagga cgagcacgta gagcgccgcg aacggcgcga cccggctatc gaggccagcg
     1681 gccaccgcca gcgaggccag gatcagctcg aactcaccac ggctgacgag catcagagcg
     1741 gtgttggagg ccgcaaggcg gccgtagccg taaacccggg cggcgacgac gccagccacg
     1801 acgttgaaca gaagcgttac cgcgacagcg accgcgaccg ccgatgcgac cgcgcccatg
     1861 tcgctcgggg cgatcgtcag gccgaaccag aagaagaaga tcgcggcgaa cgtgtcgcgc
     1921 agaggaacga gcaggcgctc tacccgcggt ccgatccccg tgccggcgac gaccatgcca
     1981 gccaggagcg ctccgaccgc gtccgaggct ccggcgatgt gggcgaagcc ggcgacgaag
     2041 acgccgaagc cgacgctgag gatgatcgta agctcggcgt cgtcggcgct tatcaggggg
     2101 cggaccaggc gcgcgcccca gcgcgcgagc gcgaacagcc cggtgagaaa cgcgaatgcg
     2161 atcgcgatcg actcgagaat ctcgtacgcg ccctgctggt cgccgaggat tggctgcagc
     2221 agcgcgaggt agaacgcaat gaacacgtcc tcgacgacga tgatcccgag gatcatcccg
     2281 gtctcgggat tcgaaacgcg gcggagatcg aggagcagct tcgtgacgat cgctgtcgac
     2341 gacgtcccga tcatccccgc gatgacgaag atctcccgcg tgccccagcc gagcgcccat
     2401 ccgagcacga gacccgcgcc gaagttgagg acgatgtagg accctcccgc ccacagcagc
     2461 cgccgcccgc cgctcatcag gtcgtcgatc gagaactcga tcccgaggtg gaagagcaaa
     2521 aggaccaggc cgagggtcgc cagcaggtgg attccctcgg ggtcgtcaag aacgacgagc
     2581 cccggcgtgt tcggtccgaa cacgatcccg gcgacgatga agaacgggat cgtcggcaag
     2641 ccgagacgcc gcccaaaccg ggcgaaaagg ccggccagca gcaaggcggc ccctaagacg
     2701 gcgagctcgt tgtccacgtc acccacccaa gcggtcaggc agcgacgatc gagggctcct
     2761 catgcgccgg acaaagtctg ccgccgctgc tgcatggcgt cgatgggtga caacccccat
     2821 acggcagccg ttgcccgaag gcctcgcccg ggttcgtctg ctacttcttt acgtgcatgc
     2881 cagagggcga gccaggggac gacgagcctc cgagtccgag ctgtagaccg gcgcctgctg
     2941 gcgatggcgg gggccgatga tcgaggttct gttcctcgtc gtgtctgctg tgctggtggt
     3001 cgcgtgcggg gcgttcgtcg cggcggagtt ctcgttggtg acggtcgacc gctcggctgt
     3061 cgagcgcgcg gccgagcgcg gtgacgccgg cgcggccggc gtcctgaaag cgttgagcag
     3121 cctctcgacc cagctgtcgg gcgcccagct cgggatcacg ctcaccaacc tcctgattgg
     3181 cttcgtcgcc gagccgacga tcgccgacct gatcgacggg ccgctggagt ctgccggagt
     3241 gccggcggcg atcgtcaccg gcgtagcgct cgtgatcgcg ctgtccgcgg cgactacggc
     3301 gacgatggtg gtcggcgagc tggtgcccaa gaacctcgcg atcgctcgcc cgctccagac
     3361 ggcgcgggcg gttcagggct tcatgcgggg cttcacccgc gtcaacgcgc cgttgctcgg
     3421 ggtgttcaac ggagccgcga acggggttct ccggtggttc gggatcgagc cgcaggagga
     3481 gctcgcgtcc gcgcgctcgc ccgacgagct gagctcgctg atcgggcgct cggccgctca
     3541 gggcacgctc gaggaggaga cggccgcgct gctgcgcagg tcgctcgcgt tcgccgagcg
     3601 tcaggcgcag gatgtgatga cgccgcgcac gcggctccac accatcggtg ccaccgagcc
     3661 gatccaggcg gtgatcgaca tggcgcgcgc caccggccgt tcgcggtttc cggtgctggg
     3721 cgaggggccc gacgtcgtcg tcggggtcgt gcacgtcaag cacgccgtcg gcgtgccgcc
     3781 ggagcgcagg gagatcgccc gggtcgccga cgcgatggtg gccccggtcg tggttccctc
     3841 gacggtggag ctggacccgc tgctcggcag gccgctctgg cgcagcctgc agatggccgt
     3901 cgtggtcgac gagttcggcg gcatcgacgg gctcgtgacg ctggaggaca tcgtcgagga
     3961 gatcgttggc gaggtcctcg acgagcacga ccggcgcgac cccgctatcc gccgcgatcc
     4021 ggcgggcggc tgggtcctgt cgggcctcct gcgccccgac gaggcaagcg acgccgtggg
     4081 cgcggcgctg cccgagtccg acgactacga gaccctcggc gggctgatcg caaccgagct
     4141 cgggcgcatc gcccacgtcg aggatcgggt gtcgctggag gtccgcgacg cggaggggct
     4201 tcgccgcgag gcgcagctga cggtcgcgcg gatggacggc tggcgcgtcg acagggtccg
     4261 gctgcggctt ggcgaggccc tccccgacga ggagcacgag tgaacgactg gctcgccctc
     4321 ggcctgggcg tcttcctgct cgcgctcaat gcgttcttcg tcggcgcgga gttcgccctg
     4381 atctccgcac ggcgcagcgc catcgagccg aaggcacagg aggggtcacg gcgagcgaag
     4441 gtgacgctga gggcgatgga gcagatctcg ctgatgctgg ccggcgcgca gctcggcatc
     4501 acgatctgct cgctgctgct gctggcggtg gtcgagcccg ccgtcgcgca cctgcttgag
     4561 ggaccgttcg gcgcgctcgg ggtgagcgac gcgctccggc atcccgtcgc gttcgcgatc
     4621 gcgctcgccg ctgtcggcgt ctcgcacgtg gtgctcggcg agatggtgcc gaagaacatc
     4681 gcgctcgcca agcccgaccg tgccgcgttg gcgctcgcgc cgccgctcct gctgatcgtg
     4741 aggctgctgc gacccgcgat cgccgcgctg aacgcgatcg ccaacgggat gcttcggctc
     4801 gtaggcgtgc agcctcagga cgaggtgacg agcagcttca cccgcgagga ggtcgccggc
     4861 ttcgtcgacg agtcccgacg cgaggggctg ctgcacccgt ccgaggagcg caccctgatc
     4921 ggagcgctcc agttcggcga gcgcacagcc accaacgtcc tgctgccgct cgacgatgtc
     4981 gagaccctgc cgatcgccgt caccccgcac gatgtcgagc gcgccgccgc ccgcaccggg
     5041 ttctcacgct tccccgtcca ggacgagtac ggcgccccga tcggctacct gcacctcaag
     5101 gacgcgctcg agaccaagga cgtccatcgc aaccgcccga tcgcgcgacg gtgggtgcgg
     5161 ccactgcccg cgatccccgc caccgacccc ctgctctcgg tactcgccac catgcagcgc
     5221 tccggggcgc acctcgcgca ggccgtcgac ggcgatcgcg ggctaatcgg gatcgtcgcg
     5281 ttggaggacg tcctcgagga gctcgtcggc gagatccgcg acggaacacg acgcgctgcc
     5341 tagggacgtc gcttgcgcaa gggccccacg cccccaccgc tcgttccgaa tcctcgtcgc
     5401 tttttggcag aacctttgga aaccgagcgc ctccctcagc gcagcgccgt caggacccgc
     5461 tcgacgagcg cgtcgagcgc gcggacggcc gcttcgaggt cctcggggcg cgtgtcctcc
     5521 tcgcgcgtgt gggagcggcc gccgatgctg cgtgcgaaga gcatcgccgc ggggacgccg
     5581 gcgcgggcga cggcggcggc gtcgtggagc gcgccgctgg agatcggcag cgcctcgccg
     5641 gcgagctcag tcacgacgtc gtcggcgatg ctgacgagca gcgggtcgaa cggcaccggc
     5701 gccgtgcgca gcagcgtctc ccaggcgact gtcgtccgtt cctcggcggc gatccggtcg
     5761 gccgcctcgc gggcgcgccg ctcgacgtcg gcgagcagct ccggggtctc gtggcgctga
     5821 tcgacgagca gcgtcgcctc gccggcgacg gcggtgacga tcccgggcgc ggcggcgacg
     5881 tcgccggtgg tgccgcgccc accttgccgg cgcgcgatct cgcgcacctc gagcgccagg
     5941 cgagcggccg agagcagcgc gtcgcggcgc agctcgagcg cggtcgcccc ggcgtgggcg
     6001 gtctcgccct cgaagcggac cgcgtggcgg acgacgccga gcacgccgct tacgacgccg
     6061 accggcaggt cgcggcgctc gagctcgacg ccctgctcga tgtgcagctc gaggtaggcg
     6121 acgacgtcgg cgagctgcgc gtgcgcctcc cccatccgcg ccagctcgac gccgtgctcg
     6181 gcgagcgcct cgcgcacgcc gaccccgtcg cggtcggcga gcgcggctag ctcctcgccg
     6241 tcgagcaggc cggctgccgc ctccgagccg atcaggctgc gcccgaagcg ggcgccgtcc
     6301 tcgtcggccc agtcgaccag ccgcagcgtc agcggcggct gcccctcgcc ggcgacccgg
     6361 cgcagcacct cgagcgccgc gaggacgcca agggtgccgt cgagccagcc gccgtcaggg
     6421 accgagtcga ggtggctgcc gaccacgacc gcacgcggcg aggcgccggg cagcgtcgcc
     6481 cagagattgc cggccgggtc gacctcgacc tcgaccggca gctcggccag ccgcacgcgc
     6541 agccactcgc gcgcgcgggc ccaggtcggc gtccacgcca gccgctgggc gccgcgctcg
     6601 tcgccggtca gggcggcgag ctcgcgcagc tcggcgaccg tccatcccgg gtcgagcgcc
     6661 atccggcgct agccgccggt gtagagctcg tcggcgatcg agagcacctc gtcgaggatc
     6721 gcgacgccct cctcgacctc ctcgtcggtg atcgtcagcg gcggcgtgac ggcgagcacg
     6781 ttccagtggc cccacatgaa caggccgcgc tcgcgcgccg cggcgagcat gcgggcgagc
     6841 ggcttgacgt cattgccgga ggcgttgaac ggcaccagca tctcgcgcgt cgcgcggtcg
     6901 cgcacgagct cgagtcccca gaagcagccg aggccgcgca cctccccgat cgacggatgg
     6961 cgatcctgca gctcgcgcag cgccgagccg atcaccccgc cgatccgcgc cgcgtgctcg
     7021 acgatgccct cgtcgcggaa cgcctcgatc gaggcgacgg ccgaggcgca ggcgagcggg
     7081 tggccgctgt aagtcagccc cccggggaag aagctctcgc gcgcccaggc ggcgaccggc
     7141 tcgcgcacga ccatcgcgcc gagcggcacg tagccggagt tgatcccctt cgcgaccgtg
     7201 aggatgtcgg gcacgacgtc ccagtggtcg accgcgaacc actcgccgct gcggccgaag
     7261 ccggccatca cctcgtcggc gatcagcagg atcccgtggc ggtcgcatac ctcgcggatc
     7321 gagcgcaggt agccgtccgg ggggacgagg atgccgttgg tgccggtgat cgtctcgagc
     7381 acgaccgcgg cgacgttctc cggtccctcg tactggagga tctcctcgag atgcgggccg
     7441 cccgagcaga ccgggcacgg gtcgggatgg ccggccgggc agcggtacgt gtaggggtcg
     7501 agcatccgca cgactcccgg gatgccggga tcggtcggcc agcggcgcgg ctcgccggta
     7561 agcgcgatcg ccccggcggt gccgccgtgg taggagcggt agcgcgcgac gaccttctgg
     7621 cggccggtgt agcggcgcgc gagccggatc gcgtactcgt tcgcctcggc gccgccgttg
     7681 gtgaagaacg ccatcgagag gtcgccgggc gtcacctcgg cgagcaggcg ggccagccgc
     7741 gagcgcggct cgttggcgag cggcgggccg atcgtgcaga ggcggtcggc ctgctccttg
     7801 atcgccgcga cgacccgtgg gtgctgatgg ccgatcgaga cgttgaccag ctgcgaggcg
     7861 aagtcgaggt agctgttgcc gtcgctgtcc cagaacctcc gcccctcggc gccggtcacg
     7921 acaagcggct cgacctgggc ctggatcgcc cacgggtaca gaacgtggtc gcggtcccac
     7981 tgcttcgcct gctcgccgct cgcgacggtc gcgccctcca tgtcagtcct ggtcctcgtg
     8041 gatcacctcg tcggcggggg tcagcaccga gatgtgcgag aggtcgctct ccgcagtcgc
     8101 tccgtgccag tggcgcgtgc cggccgggac gatcacgacg tcgccggtgc ggaccgggat
     8161 ctcctcgtcc tcgctggcca ccgtcccgtg cccgccggtc acgatcagca tctggtcgca
     8221 gccgtggtgg tgccactggg tccgtgcacc gggccggaag ctgatgtgcg ccgagcggaa
     8281 cagctcggcg ttctgctcgt tggcgatcgg ctggtagtgg acctcgccgc ggaacatctc
     8341 ccagtcgctc tgctcgccga tcggccgtcc gagcgtgact ctgtgcatcg gctctccttt
     8401 ggtcctatgg ggtggtcgag gcgacggtcg ccgccgtggt cggtcgcgcc gcgcgctggt
     8461 ggacgcccgc gaggaaggag aggatcagct tcatccccag ccgcgaggtc atgtcgccga
     8521 agtcgcgcgt cgggtcgatc tccgacacct cgagcgccac gacgtcctcc tgcaggccga
     8581 gcgcgtgcag cgcctcgatc gcctgccagg catttagccc gccgctgatc gaggcgccgg
     8641 tgccgggtgc gtaggcgagc tccatgcagt cgatgtcgaa cgtcacgtag acggcgtcgg
     8701 tgccgtcggc ggcgatcgcg tgcgcctcgg cgacgatctc ctcgatcttc ccgcgcgcga
     8761 cgtcccgcgc gctgaagacg gtcgtcccct gctcgcggac gtagtcgcgg tagtagccgc
     8821 cgttgacgaa gcccgagatg ccgatctggg cgatgttgcg cgcctcgacg ccgatgccgt
     8881 cgccgagcag gccgcgcagc ggcgtgccgt tgtgcggccc gcagaagtcg agcacctggg
     8941 cgtcgaggtg ggcgtcgaag tggacgatcc cgatccgctc gacgtcgggg cgcccggcgg
     9001 cgagcccctg gacgatcggg cgcatgatcg agtggtcgcc gccgacgaag accggcacgt
     9061 aggccggccg gtgggcgaag atcgcggtcg ccgcctcggc gacgttggcg tgcgcctgct
     9121 cgacgtctat cagcggcacc tcgacgtcgc cgaggtcgcg cacgaccatg tcgcgcaggt
     9181 cgaagtcgaa gtcgatgctg taggtcgtga agaagaaccc cgtctgacgc acagcgttgg
     9241 gtgcgtagtt ggcgccgacc tgcttgatcg cagcgcgcgc gagcggcacg ccgacgatcg
     9301 cggcgtcgat ctcctcggcg ccgtcccact cgcggatcca gttcgccacg cggacctcgt
     9361 tggggtcctg ccagtgactc ctggtgacga tctccgccgg ccgcatccgc ggcactccgt
     9421 tgctgctctg cgtcatgcct ctcccttttg tcgcgacagc tgtccggtcg ccgctaggcg
     9481 acgacctctc cctccgggac gacctcgccg cggatcaggt tgaggatccg gtggcggagg
     9541 gcgccgaagc gcgggtcctc ctcgatctcg cgtgtgcgcg gacgcgggaa ctcgacctcg
     9601 atccgctcgc agatccgccc ggggcgcgta cccatcacgt acaggacgtc gccgaccatc
     9661 agcgcttcgg cgatgtcgtg ggtgacgaag acgatcgtcc ggcgatcggc ctcccagacc
     9721 tcggtcagga agtcctgcgc gagcgtccgg gtcagcgcgt cgagcgcgcc gaacggctcg
     9781 tccatcagca gcacgcgagg ctcgttggcg aacgcccggg cgatcgccac gcgctgctgc
     9841 atgccgcccg agagctcgcg cgggtaggcg tcggcgaact cggtcaggcc catccgatcg
     9901 agcagccgct cgctgatccg cgcccgctcg gcgcgcggga cgcccgcgag cttcggcccg
     9961 aactcgatgt tcgcccgcac cttcagccat gggaagagcg tgtaggtctg ggcgaccatc
    10021 ccgcgatcgc gacccggctc gtggacctcc tcgccgtcga ccgcgacgct gccgctggac
    10081 ggattcgcga gcccggcgat cacgttgagc agcgtcgact tgccgcagcc cgacggaccg
    10141 agcaggcagg cgaactcgcc gtcggcgacg gtcagcgaga cgtcctcgag cgcctcgatc
    10201 cgccccaccc gagtgtcgaa gcccatcgac acgccgtcga tctcgaccac gctcatcgcg
    10261 ccgtcctctc cgcgtagggg aacagcattc ggtaggccca gcggaagagg ccgtcggtga
    10321 tcaggccgag cgcgccgatc gtgaggatgc cggcgatcac ctgatcggtc gagagcgtcc
    10381 ggctcgacgc gaggatcacg tgcccgatcc ccgagctggc cgccacgagc tcggcgacga
    10441 tcaggtacgt ccaggcccag cccatcccga tccgcaggtt gtcgagcacg ccgggccagc
    10501 aggccggcag cagcaccctg gtgaacacct tgcgccggct ggcaccgagc gtgtaggaga
    10561 tgttcagcag cgactgcggg acgttcgccg agacgtcggc gatcagcagc gtcagcgaga
    10621 agaagacgcc aaggaagatc accgccacct tctcttgttc gccgatcccg agccagagga
    10681 tcagcagcgg gatgaacgcc gacgccggca tgtagcggat cgccgccagc gtcggctcga
    10741 agtacgactg gacgacgcgg aacgagccga tcgcgatccc gagcggcacc gcgatcaccg
    10801 cggcgagcag gaagccgagg ccgatccgcc cggcgctggc gccgatgtcg ctggcaaacc
    10861 cctgcttggt gatcatgtcc caccatgccg acgcgacggt ggtcggcgtc ggcaggaaga
    10921 actcgtcgta gacgccgccg taggacagtg ccgaccagag cgcgatcacc gtcaggccgc
    10981 cgccgaaggc gagcgccgtg cgcatccagc gcgggatcgg cgcccgcggc gtcaggtacg
    11041 actgccggcc gcgaccgccg ccgcggcgac gccggggggc gcgccgcggt tgcggcagcg
    11101 cgcccgctcc ggcggccacc acgggaggct gagaggcgcg ctcggtcatt gggcgttgac
    11161 gaagctcggg tcgatcccgt cctcggcgct cggcgtcgag tcgatgtcgc ccgcctgctg
    11221 ccagatctcg cccgcggcct cccacagctc gtagatccgg cccggctggt cctcggtgcc
    11281 gaagaactcc tgattctcct tggcgccgta gagccgcagg tcgccgagga tcgccgtgac
    11341 gtcgtccggg ctctggtcgg tcgcgcgggc gatgatcgcc gcggcctcct tcgggttgct
    11401 ccgcaggaag ctgatcgcgc ggtccgtgcc ctcgaccagc gcctggaccg actcggggtt
    11461 ttcctcgatg aagtccgagc tcaacccgac ggtcgtcacg acgacggggt actccttggt
    11521 gtcggcgagg acgtgcccgt tcgggctctc ctcggcgcgc gacagccacg gctgccaggt
    11581 cgccgcagcg gggacgctgc cgccggcgaa ggccgcgccg gcgtccgccg ggctcatgtc
    11641 cgccttgtcg acgtcgtcga gcgtcaggcc ggcctcgcgc agcacgtagc cgagcaggaa
    11701 ctcggcggtc gagccgtcgg cgacgccgac gcgcaccccg cgcaggtcgc tcggctcggc
    11761 gatgtcgtcg cgcgcgacga tgccgtcgcc gccgaacgac tcgtcggtgg cgagcacctg
    11821 cttggcctgg acgccctgtg ccgcccagcg cacccaggtg tccgcggtca tgccggcgcc
    11881 gtccttctcg cccgcctgca tcgcctgcac gcggttggtc tggtcctcga cgaggtcgag
    11941 gtcgacctcg aggccggcgt cgcggtagta gccctgctcg tcggcgatgt agagcgcgcc
    12001 gaagccgggc caggaggaga gcgccagcct caccggcgcc atgccacctt cgccgcccga
    12061 gcctccgcct ccccctccgc cgtcgtcgtc actcccacag cccacggcca acgccatcag
    12121 aatcgccacc agcgtggtgg cgaacagctt tccggtcatc gcagctctcc ttgtcctcgg
    12181 gatcggcgtt cgaggttcac ccagaccgac ttcgtctcga ggtgctgatc gaggacctcg
    12241 ccgccatgct cacgccccca gccgctctgc ttgaagccgc cgaacggcac ggcggggtcc
    12301 gtctcgttgt aggtgttgat ccagaccgtc ccggcctcga gcatccgcgc cagatcgtgc
    12361 gcggccgcca cgtcgtgggt ccagatgccc gcggcaagcc cgtactcggt cgagttggcg
    12421 cgcgccgcta cctcctcgag gctctcgaat ggcagcgcta cgagcaccgg gccgaagatc
    12481 tcctcgcgcg cgacgcgcgc gtcgtcggag accccggtga tcacggtcgg ctcgacgaag
    12541 tagccgcccg gcgggcggtc gccggccggc gcgcggtgga cgtcgccgcc ggcctcggcg
    12601 cccgcgccga cgtactcgag cacgcggtcg cgatgctggg cggagatcag cggccccatc
    12661 gtcgtcgtct cgtcgaggcc cggcccgagg cgcacctcgt cggcgctgtc gcggaccccg
    12721 gcgacgacgt cgtcgaagat cgagcgatgg acgaacaggc gcgagccggc gatgcagttc
    12781 tcgccctggt tgaggaagat cgccgcggcc gcctcaggcg cgatctcggc cgggtccatg
    12841 tcgggcagga cgatgttcgg gctctttccg ccgagctcga gcgtgacgcg cttgaggttg
    12901 ccggcggcgg cgcgcacgat ctcgcgcgcg acctcgggcg agccggtgaa cgagaccttc
    12961 gcgacgccgt cgtggcccgc gagcgccgcg cccgcctcgc cgccgtaccc cgtcacgacg
    13021 ttgacgacgc cgggcggcag cagatcggcc tcgcgcagca gttgcgccag gtagaggacg
    13081 ctcagcggcg tctgctcggc gggcttgacg accgccatgc agccggccgc gagcgccggc
    13141 gcgagcttcc aggccgcgat catcagcggg aagttccacg gcaggatctg cgcgacgacc
    13201 ccgaccggct ggcgcagggt gtagatcagg cgatgctccc cgggaccggg gatcgtcgag
    13261 ccctcgatct tcgtcggcca gccggcgaag tagcggaaga actcgaccgt cttggcgacg
    13321 tcgtagtcgc gcgccgcggc gaccggcttg ccgctgtcga ggctctccaa ctgggcgaat
    13381 gtctcgccgt gctcttcgac taggtccgcc agcgcgtgta gcgtcatcga ccgctgatgg
    13441 gcacccatgc gcctccacgg gccggacatc gccgcgcgag cggcctcgac ggcctggtcg
    13501 acctcccgcc tgccggccgc cgggacctcg gcgacgacgg ccccttgcgc cgggtcctcg
    13561 accgggatcg tctccctgtc gacggcgatc tcgcgaccac cgatcgagag cggcagcggg
    13621 ccggcgccga ggaagtcgcg tacgccctgc ccaagccgtt cttcgtaggc atcgagggct
    13681 ttaccgtcgg agcgagcagc ctccggcgat tgcgaacccg atggcggtga ttcgtgtccg
    13741 ctgaccactg tagtctaaaa tatcagatct aagatgttag atgcaaataa ttccggtcaa
    13801 gctgggcccg tcgtcgcggc cccgttgcgc gaccaggtct acgagcggct gcgcagcgcg
    13861 atcctcggcg gcgagattcc ggctggcgag cgcgtctcgc cgcccgagct cgccgagcgg
    13921 ctcggcgtca gcacaacccc ggtccgcgag gcgctgcgct cgctcgagga ggaggggctg
    13981 atcgagacct cgccaaggcg ctggacgcgg gttgcgagcc cgagccccga gctcgccgcc
    14041 gagacgtacc cggtgatcgc gacgctcgag gagctcgccg tcgcctcgat cgagaccgtg
    14101 acgcccgacc tgctcgaaga gctgcgcgcg gccaaccgcg acttcgagcg cgccgccgcg
    14161 gacggcgacg tcttcgcctg catgaccgcc aacgatgcct ttcacggggc gctcctgcgc
    14221 gccaacggca acgggacgct cgagcgaacg ctgcgcgacc tcaagacccg gatccggttg
    14281 ctcgacagcc agtacttcca gtccggcacc gagacctcga tccgcgagca cgagcaggtg
    14341 atcgacgcgc tcgagcgcgg cgacatcgag caggcggccg gggtgattcg cggccagtgg
    14401 gtgcgctggc tcccggcgtg gggcgtgtcg ccggccgaac actaggcgcc gaccgcggtc
    14461 cgttctgcgc ggcgcgcccc cgcgaaggcc tcggcgaccg gctcgcagac gatctggccg
    14521 ccggcgacgt tgagcccctt ggcgagcccc ggatcgccgt cgatcgccgc ctcgacgcct
    14581 aggtcggcga gccgctggac gtatggccgc gtcgcgttgg tcagcgcgcg cgtcgaggtc
    14641 accggcaccg ctccgggcat gttgctgacg cagtagtgga cgacgccctc gacctcgtag
    14701 acggggtccg cgtggctggt cggcacggcg gtctcgacac agcccccctg gtcgatcgcg
    14761 acgtcgacga cgacggtccc cggccgcatg tccgacagca tcgctcgcga gatcaggcgc
    14821 ggcgcgcggg cgccggggat cagcaccgcg ccgatcacca cgtcggcgtc ggcgacctgc
    14881 tcggcgatcg agccggcgtc cgacatcagc acgcgcgcgc gagtgtcgaa gtaggcctca
    14941 agctcgcgga tccgcgccgg cgagcgttcg agcactgtca cctgggcgcc catcccgacc
    15001 gcgacgcggg ccgcctgggt cccgaccgcg ccgccgccga cgaccagcac gcgcgccggg
    15061 tcgacgcccg ggacgcctcc gatcaggacg ccggagccgc ccaactggct ctgcatgaag
    15121 taggcgcccg cctgggtcgc gagccgtccg gcgacctcgc tcatcggcgc gagcagcggc
    15181 agccggccgt gctcgtcctc gatcgtctcg taggcgatcg cggtggtgcc ggagtcgacg
    15241 agcgcctggg tcaggtccgg gtcggccgcg aggtggagga aggtgaacag cgtcaggccc
    15301 ggatggagcc ggcggtgctc gctcggcgtc ggctccttga ccttgagcag cagctcgctg
    15361 tcggcccaga cctcgtcggt cccggcgatt cgcgcgccgg cggcctcgta gtcggcgtcg
    15421 gcgtagcgcg agccggcccc ggctccgcgc tcgacgatca cgcggtggcc gctcgcgacc
    15481 agctcgcggg caccgcccgg ggtcagcgcc acccggcgct cggcggtctt ggtctccttc
    15541 gcgacgccga cgagcactta ggcggcctct ccccccaccc caccggacgc gaggctcgcg
    15601 gtgaggccca tctcgtcgcc cgcgccggcg agcaccgcgg cgacgccgtc gacgacctcg
    15661 tcgaggaacg cctcgtcggc gatcagcggc ggcgcaatct ggaggaccga gtcgccgcgg
    15721 tcgtcggcgc gcgcgatcag cccctcctcg agcagccgcc gcggcatgaa cccgcgcagc
    15781 aggcgctcgc gctcgggcgc gtcgaagcgc tcgccgccct ccttgacgag ctccatcgcc
    15841 cagaagaaac ccgcgccgcg cacgtcgccg acgatcggca ggcagccgac ccgctcgcgc
    15901 agcgcccgct cgaggtagcc ctcgaggcga cggacgttcg cgagcacgcc ctcgcgctcg
    15961 aagatctcga tgttgcgcag cgcgatcgcc gccgacgccg ggtggccgcc gaaggtgatc
    16021 ccgtgtagca ggacgcgctt gtcgtcgtag agcggcgccg cgacccggtc gctgcacagc
    16081 acggccccca tcgccgcgta ggccgacgtc aagcccttgg cgacggtgat caggtccggg
    16141 cggccgccga agcgctcgac ggcgaaccac tctccgagcc ggccgaagcc ggagatcacc
    16201 tcgtcggcga cgagcaggat gccgtggcgg tcgcagatct cgcgcacgcc cggccagtag
    16261 ccgtccggcg gcgtgaagca gccgccggcg ttctggatcg gctcggcgat gaacatcgcg
    16321 atcgtgtccg ccccctcctc ctcgatcgtc gcctcgagct cggcgagcag gtcgcggcag
    16381 agctcctgct ccgagcgctc gtcgcggaag gagttggtgt tcgagacgtg acgagtcggg
    16441 atcgccggct cgccgaacgg ctgcttgaag gcctcgactc cggtcagcgc cagcgcgccg
    16501 agcgtgacgc cgtggtaggc ggtgcggcgg gcgatcgcct tggtccgctg cggctcgccc
    16561 atcgctacgt ggtactggcg gacgatcttc caggcggcct cgaccgactc cgagccgccg
    16621 ctggtgaaga ggaccttgcc gatgccctcg ggcgcgagct cgacgagccg gtcggcgagc
    16681 gcgagcgccg gcgggtgcgc agtcgcccag ttcgtgttaa acgccagccg ccgcagctgg
    16741 tcgccggcga cctcggccat ctcgtcgccg tgcgagtagc cgatctggca gcagaacagg
    16801 ctcgagagcg catcggcgta gcgccgtccc tcggtgtcga agacgtacgg gccctcgccg
    16861 cgctccagca cgagcaggtc ggacgggtcg gcgaaggcgc cgttgcggct gaagtgcagc
    16921 agcagccggc ggtcgctcat gcccatcgcg aggtcaccac cttcttgcgc gtgtagaact
    16981 cgaacgcatc ggggccgttg gcgtgcaggt cgccgtcgaa ggagtccttc cagccggcga
    17041 acgggaacca ggcgaccggg gcggcgacgc cgacgttgac gccgaccatc cccgcctcga
    17101 ccgagtagcg gtagctgcgc gcggcctggc cggacgaggt gaagatgacc gacgcgttgc
    17161 cgtagcggct ggagttgacg atctcgatcg cgtcgtcgag gtcgcgtgcg cgcagcagcg
    17221 tcaggaccgg gccgaacagc tcctcggtca ccgcacgcga gtccagcggc gcgcggtcga
    17281 cgatcgtcgg gcgcagctcg gtgccgccgg cgccggacgc cccgcggcca tcgaggacga
    17341 tctcggcctc ggccgcgagc gcctcgatct cgccctcgag ccgctcgcgc gcctgagcgc
    17401 tgacgagcgg gcagacgtcg gtctgcgggt cggcgccggg tccggtgcgc agcgcctggg
    17461 ctccggcgac gatccgctcg cgcgctcggt cctgctcctc gggggtgccg acgagcacgg
    17521 cgatcgagcc ggcgaggcag cgctgcccgg ccgcgccgaa tgcggacgag aggaccccgt
    17581 cgagcatcag ctccggatcg gcgtcgggca tcacgaccat tgcgttcttc gccccgccga
    17641 gcgcctgcac gcgcttgccg cgcgtcgcgg cgcgctcgta gatgtagcgg gccgtcttcg
    17701 ccgagccgac gaacgagatc gcgtctacgc ccgggtggtc gagcagcccg tcgacggcct
    17761 cgtgcgctcc gttgacgagg ttgaccaccc cgggcgggaa cagctcggcg tcggcgatca
    17821 gctcgaacat ccgcaccgag gtcagcgggt cggcctcgga cggcttcatc acgaccgtgt
    17881 tcccgcagac gaccgcgtag ggcaggaacc agagcgggat catcgccggg aagttgaacg
    17941 gcgtgattcc cgccacgacg ccgaccggct ggcggacgag ctcgacgtcg actcccgtcc
    18001 cgacgccctc gaggttctcc cccttgagca tgtgcggcgc ggcgcaggcc gcctcgactg
    18061 actcgatccc gcgaccgacc tccgcccgcg cgtcggcggt cgtcttgccc atgtcgcgcg
    18121 tgacgagctc ggccagctcg tcggcgtggc gggtgagcag gtcgcgcagc ccgaacagcg
    18181 cccgcgcgcg gcgcagcggc gaggtcgcgc gccagcccgg gaacgcgtcg cgcgccgcgc
    18241 ggaccgcccg gtcgacgtcg tcggcgccgg acagcggcac cgtagcgatc agctcgccgg
    18301 aggctgggtc gaggcagtcg atggcctcgc gctccgggag ctcgacccag cggccgccga
    18361 cgaagttgcc caggcgcggc gccgcggcgt cgtcggaacg ggtgaccgag tctgcggcgg
    18421 gagcgatcga agatgtgttc ggcattgggt cgatggtcgc gccccgcgca agctgcgaca
    18481 agatccgatc cgctcaactt cagccagttc gtttgacgtt ctgtcagatg aaacagcgag
    18541 gtggacgctc tagcctgcgt ccatctgaca gttgatgcca ccgcaaccga gccgtccctg
    18601 gtcaccgtcg ccacggtgct cgcgacccct gagctgcagc gcggggttcc ggaggtcgtc
    18661 gccggggcgg acgggctcgg gcgtccggtc cgctgggtgc acgccgccga gaagccggac
    18721 atcgccgcgc tgctcaaggg cggcgagctg ctgctcacga cggggctggg gatgcagcgc
    18781 gccgccgccg accagcgccg gttcgtgcgc gagctgtcgg cacgctcggt cgccggcgtg
    18841 gtgatcgagc tcggcagccg tttcgacagc gttcccgagg cgatccgcga cgaggccgag
    18901 cgcgcgacgc tgccgctgat cgcgctccac cgcgaggtgc gcttcgtcga catcaccgag
    18961 gcgatccacc gcgagatcgt caaccgccag ttcgagctga tgcggcgcgg cgacgaggtc
    19021 catcgccgct tccgcaacct gctgctcgac ggcgccggcg tccccgaggt gctcgacgcg
    19081 ctcgccgagg tggtcgacaa cccggtgatg ctcgagcgcg ccgggcacgg gctgctctac
    19141 cactcggcgg gccggtcggg cgagccggct gcgctggcgg gctgggcgtc ggtcgccggc
    19201 gggctcggcg gcgcgccgca gttcgtcgag caggcgatcc ccgtcgcggg cgacgacgcc
    19261 ggcggccgcg tcgtggcgat cgggatcgac tcaccgctcg acgagtcgga tcgcgtcgcg
    19321 gtcgagcgcg ccggcgagct gatcgccctg gcgctgctgc gcgaccgcga ggaggaccgc
    19381 ctggtcgggc gcgagcgcgg caacttcctg ctgtcgctgc tgcgcggtga ggcgatcgac
    19441 gagcgcgccg cgggcgagcg cgcgcgggcg ctcggcttcc cccacgccgg gcggatgctg
    19501 ccgatcatcg ccgcgcgctc ggtctcggca tcggcggcgg cgggcagcga ggcgcgcgtc
    19561 tggagccggc tctggagcga cgtgctcggc cggctagcgg atcggcggtt ggccgtgctc
    19621 gcggggacgc gcgagcacga gcgcgagctg ctctgcgtcg tcggcatccc gggcagggcc
    19681 gaccgcaccg agctgatgga ccgggtggcg gcgatcgcgc acgaa
//
LOCUS       DATABV010000106        12208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_996073, whole genome shotgun sequence.
ACCESSION   DATABV010000106 DATABV010000000
VERSION     DATABV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996073"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VHF88_04470"
     CDS             complement(<1..1220)
                     /locus_tag="VHF88_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2161059.1"
                     /translation="MFERFTERARQVVVLAQEEARILKHNYIGTEHILLGLLREEEGL
                     AARVLESLDITVERVRAQVVRIVGSGEEVTSGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADSEKIRNEVIRMLSGPGGRQRQGSGQGAQQG
                     EGKKSSKLLDQFGRNLTKLAAEGKLDPVVGRETEVERIMQILSRRTKNNPVLIGEPGV
                     GKTAVVEGLAQRITSGEVPELLKNKQIYTLDLAALVAGSKYRGEFEERLKKVMKEITQ
                     RGDIILFIDELHNLVGAGAAEGAIDAASILKPALARGELQTIGATTLDEYRKYLERDS
                     ALERRFQQIRVEQPSTEETVQILKGLRDRYEQHHRVGITDESLEAAAELADRYISDRF
                     LPDKAIDLIDEAASR"
     gene            complement(1290..2717)
                     /gene="lysS"
                     /locus_tag="VHF88_04475"
     CDS             complement(1290..2717)
                     /gene="lysS"
                     /locus_tag="VHF88_04475"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX2161060.1"
                     /translation="MDDRREKLQQMRADGIDPFPHAYPGVVASGAVREAHGELEDGEE
                     TESAYRVAGRLAARRGHGGQAFLDIVDRSGRIQIQAKRDVLGEESFDALTSLDLGDLV
                     GVDGTAFKSRRGELTLRATGWTLLAKSLLPPPDKFHGLEDVETRYRRRELDLIANEDA
                     RELFILRSRVIAAIRRWLDERGFLEVETPVLQPLYGGALARPFTTHHNALDRYLYLRI
                     ATELYLKRLIVGGLERVYELGKDFRNEGLSPKHNPEFTMLEWYEAYADYQDIAAELEQ
                     LVAFVADEVGYAGEVDFATPWRRVTLRDAIEERTGVDVLAHRDRDALVAAAKERGIGL
                     DPTETWPQLVDDLLSKHVEPELRNPTFITDYPVELSPFAKAHRSEPGLVERFECFAGG
                     MEFANAFTELNDPDEQRARFEEQRRHSAAGDTEAQPYDEDFVRALEQGMPPTGGIGIG
                     IDRLVMLLSGRASIREVVLFPALRT"
     gene            complement(2789..3268)
                     /gene="greA"
                     /locus_tag="VHF88_04480"
     CDS             complement(2789..3268)
                     /gene="greA"
                     /locus_tag="VHF88_04480"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX2161061.1"
                     /translation="MPRETLLTPNGLADLKQKIEHLSTVRRREVAARIKEAREFGDIS
                     ENSEYDDAKNEQAMLEKQIAELEEKLRNARIIDEKNVDTETVAVGVLVHVKDQKTDKS
                     TKFKIVGSADADPAEQRLSNESPVGKALIGHKRGDVVSVPVPRGPARKLKITKIEAA"
     gene            complement(3375..4127)
                     /locus_tag="VHF88_04485"
     CDS             complement(3375..4127)
                     /locus_tag="VHF88_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161062.1"
                     /translation="MTDGRLVRSRADLRRWLGWSLVAGLSIAALTASAAVVQGSLDET
                     DGRVIGTSLGFAIFSVTGAAGMSLRMRRPAEWSQALGAVTAAAAAAAFLLLVAAVWID
                     YDGGGLWQWWGVAALLSLGTSHASLVVGARSGSDAPIVDTLSHVSLATGAIDTSIAIL
                     LVAEAIHDGPVELFALLVIALVFSTALTPILRRLTAPGAAGQSPAPRQPDVAGELLEI
                     AARVELLAQAAGTRTGELRRHADALRASAKRL"
     gene            complement(4124..5119)
                     /locus_tag="VHF88_04490"
     CDS             complement(4124..5119)
                     /locus_tag="VHF88_04490"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEX2161063.1"
                     /translation="MRSLKDPWTLAGIELPNRLVLAPLAGIGNWFVRLQAKRHGAVLV
                     VSEMVSSFGLSYGNERTVDQFLRIHPEEHPVSMQLFGHDADVMREAAAMVAEAGADLV
                     DLNMGCPVRKVCKTGAGAALLDDPDKAVAIAKAAGEGSGLPVTVKLRPGQRPGDRAGV
                     HLARRLVDEAGVAAISFHPRHASQQHRGKPDYDLAREVVEALDVPVIVSGGLLSDERA
                     VEAFERSGAAAVMLARGSLGNPWMFERLLGLREGEPAAAEVIDELRWTIDRCEEHLGP
                     DRAGHYLRKFYPWYAERLALVKDAKQELVTAPTTAAARVALDRLLASGTAGTLAA"
     gene            complement(5130..5897)
                     /locus_tag="VHF88_04495"
     CDS             complement(5130..5897)
                     /locus_tag="VHF88_04495"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX2161064.1"
                     /translation="MLLAVDVGNTQTHVGLFRDEELLEHWRFHTSRTATADELAVVLG
                     GLLRLRENSLRDVDAVVVSCVVPSLQQEYDEVRRRYLRGEGALVGPELKTGMPIRIDV
                     PRELGADRLVNAIAAYDRCGGPCIAVDFGTALNYDVVSAAGEYLGGVISPGVEISIEA
                     LTQRAAALPKVRLEPPRAAIGKGTREAIQSGVIYGYAGQVDGLLARLRDELGDEATTI
                     ATGGWANVIVPHCEQVDELDDLLTLKGLRLIWDRNRP"
     gene            complement(5972..6589)
                     /locus_tag="VHF88_04500"
     CDS             complement(5972..6589)
                     /locus_tag="VHF88_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2161065.1"
                     /translation="MTGASVDRRRLRVLIVDDHEVVHWGFRLLLTEQPWVERCLTARN
                     GDEARELARRYEPHVALVDLFVGEESGAEMCEGLRRESPTTRVLLISGAGWISPQAAK
                     AAGASGFVSKDWAADDVAAAVRAVGNGRTVFAAAKEQPRAPLSPRERDVLGLLVTGAT
                     NREIAAQLHLSPHTVKEHTSSVYRKLEVRNRAEAVQRAERLGLTA"
     gene            complement(6586..7722)
                     /locus_tag="VHF88_04505"
     CDS             complement(6586..7722)
                     /locus_tag="VHF88_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2161066.1"
                     /translation="MPEASASLSPTEPLDTMVELLAGLDADGGSGGRAFYDRLCEAMC
                     RLTSMERAGLLLYEEARRIVVPAGSHGFDPELVGGVHGTLEETPIAQRALAEDRVVAT
                     DGDLVGQVPARYAALPGITTMTCTPVSAGGRWLGVIFADRGGPRFELTERERHTMWTL
                     GKTAALAASARIAATQHERARLLSERVDLAREVHERVMQRLFGVSLVLGAEAELTADQ
                     RRRCADEIQAAVADLRHALARPVAPPRPDIGATLREELERLGSSYDGPPLDVEWDPAV
                     EVPQELEQLSQSVLAEALRNAHKHANPTRVTVRVGARDGTFVLEVVNDGVDGGRRGRG
                     AGMGLRLAALDALQSGGVVDFGPTGDGHHDWRVRLVVPTDGQSG"
     gene            complement(7745..7969)
                     /locus_tag="VHF88_04510"
     CDS             complement(7745..7969)
                     /locus_tag="VHF88_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161067.1"
                     /translation="MESTETANFRGDDQACLVEASLACRVCLSGQIDWSLRVEEWDHE
                     VVCRCRACGDERVVSLTGDQALRLHLASRR"
     gene            complement(8044..8925)
                     /locus_tag="VHF88_04515"
     CDS             complement(8044..8925)
                     /locus_tag="VHF88_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161068.1"
                     /translation="MRYARPSFLVPAVAAAVVVAVPASAQAAPVGESLYVQQAGAGAL
                     GAKRLVLRDVPRRTVRFDDRPRRTGGTMPLRRFVRQWRSSFRGAPPNAALVVHGAPAG
                     RDIALLELRRPRYAPKRRTLTFAVRRLRQTGSSQLKGFGRRSDGSRVKRFGSASLFID
                     PSSAEPVPTIVSVRMPASAQVELSFSNTSLSYDTDGVILTNLNTPAFRQSQDFWVTST
                     GSARLGARSMSFNTSPGGGNGLSIFVLAAIDPPSSGTTLTGTATVPSGANVVLEFPGG
                     SGQRIANGPFAIPYPTD"
     gene            complement(8998..9483)
                     /gene="folK"
                     /locus_tag="VHF88_04520"
     CDS             complement(8998..9483)
                     /gene="folK"
                     /locus_tag="VHF88_04520"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX2161069.1"
                     /translation="MRGYLGLGSNEGDRLGALRAARDELERRGVTVVASSAVYETAPQ
                     GEVLDQPDFLNACLAIDTDLDPEQLLDACKDVERALGRRPGGPRHGPRPIDVDVLLLG
                     GLTHRSERLTLPHPEVTARRFVLEPLLELDPDLALPDGTRLAEAADAVADQLVRRREA
                     L"
     gene            complement(9530..9973)
                     /gene="folB"
                     /locus_tag="VHF88_04525"
     CDS             complement(9530..9973)
                     /gene="folB"
                     /locus_tag="VHF88_04525"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX2161070.1"
                     /translation="MIDDRDPIDPDDEDLDERDFDEDDEVPEPTVTVEITGLSLYTQH
                     GVSDAERELGQRLVIDVAFELADCDAMVTDRVEDTVDYAEVCEQVALAAQERSYKTLE
                     RLCAAIADRLMSRYGADSVRVKAAKPEPPIPLPVDEVSVEVWRES"
     gene            complement(9983..10810)
                     /gene="folP"
                     /locus_tag="VHF88_04530"
     CDS             complement(9983..10810)
                     /gene="folP"
                     /locus_tag="VHF88_04530"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2161071.1"
                     /translation="MTPAYSVMGVVNVTPDSFSDGGLYIDADAAVAHGEELADEGAEI
                     LDVGGESTRPGAEPVSEAVELERVAPVVERLAAAGAARISIDTSKLAVAEAALEAGAT
                     IVNDVAAFRFAPELAGLVADRGASCCLVHMQGDPRTMQADPRYGDVVAEVREFLEQRL
                     AFAVGEGVAEEKVWLDPGIGFGKTVEHNLELLRRLDEICAIGRPVVVGTSGKGFIGQI
                     TGRPAGERLAGTIATNVMALERGASVFRVHDVGPVRDALAVAAATVGRSGGPAADLA"
     gene            complement(10979..>12208)
                     /locus_tag="VHF88_04535"
     CDS             complement(10979..>12208)
                     /locus_tag="VHF88_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2161072.1"
                     /translation="RDLFEQAKQNSPCIIFMDEIDAVGRHRGAGMGGGHDEREQTLNQ
                     LLVEMDGFTMTDNIILIAATNRPDILDPALLRPGRFDRQIVVDRPDRKGRAKILEVHT
                     RGKPLAKHIDLDNLAAQTPGFTGADLQNLVNEAALLSARQGKREIDHLQLEEGIMRVI
                     AGPEKKTRVMSEKERRITAYHEMGHALVAHFLENTDPVHKISVISRGQALGYTISLPT
                     EDKFLTTRAELQDTMAMTLGGRAAEELVFGEVTTGASNDIEKVTATAKQMVMRFGMSE
                     KLGPRVFGHDHGQPFLGREYSSEPDYSDDVAREIDDEIRRVVEEAHQSARNILEQHRD
                     DLSHTSEILLRRETIEREEFIQLLEGKTELEVFGPDEPVLPSGTPAEPVEEPAARKPE
                     RAPKPLPRPGLAGGTAD"
BASE COUNT         1731 a         4409 c         4221 g         1847 t
ORIGIN      
        1 cgcgaggcgg cctcgtcgat caggtcgatc gccttgtccg gcaggaagcg gtcggagatg
       61 tagcggtcgg ccagctcggc ggccgcctcg agcgactcgt cggtgatccc gacgcggtgg
      121 tgctgctcgt agcgatcgcg caggcccttg aggatctgga cggtctcctc ggtcgacggc
      181 tgctcgacgc ggatctgctg gaagcggcgc tccagcgcgg agtcgcgctc gaggtacttg
      241 cggtactcgt cgagcgtggt cgcgccgatc gtctgcagct cgccgcgcgc cagcgcgggc
      301 ttgaggatcg atgccgcgtc gatcgcgccc tcggcggcgc ccgcgccgac gaggttgtgc
      361 agctcgtcga tgaacaggat gatgtcgccg cgctgggtga tctccttcat caccttcttc
      421 agccgctcct cgaactcgcc gcgatacttc gagccggcga ccagcgccgc caggtcgagc
      481 gtgtagatct gcttgttctt cagcagctcg ggcacctcgc cgctggtgat ccgctgggcg
      541 agcccctcga cgacggccgt cttgccgacg cccggctcgc cgatcagcac cgggttgttc
      601 ttcgtgcggc gcgagaggat ctgcatgatc cgctcgacct cggtctcgcg accgacgact
      661 ggatcgagct tcccctcggc ggcgagcttg gtcaagttgc gcccgaactg gtcgagcaac
      721 tttgagctct tcttgccctc accctgctgg gcgccctggc cgctgccctg gcgctggcgg
      781 ccgccgggac ccgagagcat ccggatgacc tcgttgcgga tcttctcgga gtcggcgtcg
      841 aagtcgagca ggatgcgtgc ggcgacgccc tcgttctcgc gcacgaggcc gagcaggatg
      901 tgctcggtgc cgatgtagtt gtgaccgagc gagagcgcct cgcgcagagc gagctcgagc
      961 accttcttcg cccttggcgt gaacgggatc tgcccagatg tcacctcctc gccggagccg
     1021 acgatccgca cgacctgcgc gcggacgcgc tcgacggtga tgtcgagcga ctcgagcacg
     1081 cgcgcggcca agccctcctc ctctcggagc aggccgagca ggatgtgctc ggtgccgatg
     1141 tagttgtgct tgaggatccg ggcctcctct tgcgcgagga cgaccacctg gcgggcccgc
     1201 tctgtgaagc gctcaaacac ggctcgagtc cttcgtctcg ggaaattgct gctgacggcc
     1261 cagtctaaca acggggtccg gctggcaagt caggtgcgaa gcgccgggaa cagcacgacc
     1321 tcgcggatcg acgctctacc cgaaagcagc atcactaacc ggtcgattcc gatcccgatc
     1381 ccgccggtcg gcggcatccc ctgctcgagc gcccgcacga agtcctcgtc gtagggctgg
     1441 gcctcggtgt cgccggccgc gctgtggcgg cgctgctcct cgaagcgggc gcgctgctcg
     1501 tcggggtcgt tgagctcggt gaaggcgttg gcgaactcca ttccgcctgc gaagcactcg
     1561 aagcgctcga ccaggccggg ctcggagcgg tgcgccttgg cgaagggcga cagctcgacc
     1621 ggatagtcgg tgatgaaggt cgggttcctc agctcgggct cgacgtgctt ggagagcagg
     1681 tcgtcgacga gctgtggcca ggtctcggtc gggtcgaggc cgatgccgcg ctctttcgcg
     1741 gcggcgacga gcgcgtcgcg gtcgcggtgg gcgagcacgt cgacgccggt gcgctcctcg
     1801 atcgcgtcgc gcagcgtcac ccgccgccag ggcgtggcga agtcgacctc gccggcgtag
     1861 ccgacctcgt ccgcgacgaa ggcgaccagc tgctcgagct cggcggcgat gtcctggtag
     1921 tcggcgtagg cctcgtacca ctcgagcatc gtgaactcgg ggttgtgctt cggcgacagg
     1981 ccctcgttgc gaaagtcctt gccgagctcg tagacgcgct cgaggccgcc gacgatcagc
     2041 cgcttgaggt agagctcggt cgcgatccgc aggtagaggt agcggtcgag ggcattgtgg
     2101 tgcgtggtga acgggcgggc cagcgcgccg ccgtagagtg gctgcaagac cggcgtctcg
     2161 acctcgagga agccgcgctc gtcgagccag cgccggatcg ccgcgatcac gcgcgagcgc
     2221 aggatgaaca gctcacgcgc gtcctcgttg gcgatcaggt cgagctcgcg gcggcggtag
     2281 cgggtctcga cgtcctcgag cccatggaac ttgtccggtg gcggcagcag cgacttcgcg
     2341 agcagcgtcc aaccggtggc ccgcagggtc agctcgccgc ggcgcgactt gaaggcggtc
     2401 ccgtcgaccc cgaccaggtc gccgaggtcg agcgaggtca gcgcgtcgaa cgactcctcg
     2461 cccagcacgt cgcgcttcgc ctggatctgg atccgtccag accgatcgac gatgtcgagg
     2521 aaggcctgcc cgccgtggcc gcgccgcgcc gccaggcgac ccgcgacgcg gtacgccgac
     2581 tccgtctcct cgccgtcctc gagttcgccg tgcgcctcgc ggacggcacc cgaggcgacg
     2641 acgcccggat acgcgtgcgg aaacgggtcg atcccgtccg cgcgcatctg ctgcaacttc
     2701 tcccgccggt cgtccacggc gcagacccta tgagatgaca tccgagggct cacgcccccg
     2761 gacccccaag gcacctggcc ggcagcggct acgcggcctc tattttggtg atcttcagct
     2821 tgcgagctgg accacggggg accggcacgg agacgacgtc gccccgcttg tggccgatca
     2881 gcgccttgcc gaccggcgac tcgttcgaca gccgctgctc ggcggggtcg gcgtcggccg
     2941 agccgacgat cttgaacttc gtcgacttgt cggtcttctg gtccttgacg tggaccagca
     3001 cgccgaccgc gacggtctcg gtgtcgacgt tcttctcgtc gatgatgcgc gcgttgcgca
     3061 gcttctcctc gagctcggcg atctgcttct cgagcatcgc ctgctcgttc ttcgcgtcgt
     3121 cgtactcgga gttctcggag atgtcgccga actcgcgcgc ctccttgatt cgagcggcga
     3181 cctcgcggcg gcgaactgtc gagagatgct cgatcttctg cttcagatcg gcgagtccgt
     3241 tgggtgtgag aagcgtctct ctcggcatag gaaaaccccc cgagcctcga ccgttgcgcc
     3301 ggagggagcg cgtgagtgta gcaaccgccc tcgccgggac cgacagggcg tcagggccga
     3361 acgccaaccc gctctcagag ccgcttggcg ctggcgcgta gagcgtcggc atggcggcgc
     3421 aactcgcctg tccgagtccc tgctgcctga gcgaggagct cgacgcgagc agcgatctcc
     3481 agcagctccc cggcgacgtc cggctgcctc ggcgccggtg attggcccgc ggcgccgggc
     3541 gccgtgagcc tgcgcagaat cggcgtcaaa gccgtcgaga agaccagcgc gatcacgagc
     3601 agcgcgaaca gctcgaccgg accgtcgtgg atcgcctcgg cgacgagcag gatcgctatc
     3661 gatgtgtcga tggcgccggt ggccagggag acgtggctca gcgtgtcgac gatcggcgca
     3721 tcgctgccac tgcgtgctcc gacgacgagc gacgcgtgcg aggtccccag cgacagcagc
     3781 gccgcgacgc cccaccactg ccagagcccg ccaccgtcgt agtcgatcca caccgcggcg
     3841 acgagcagga ggaacgcggc ggccgcggcc gcggcggtca ccgccccgag cgcctgagac
     3901 cactccgctg gcctccgcat gcgcagcgac atcccggcgg cgccggtgac gctgaagatc
     3961 gcgaagccga ggctcgtacc gatcacccga ccgtcggtct cgtccaacga gccctggacc
     4021 accgctgccg aagcggtcag cgcagcgatc gacaggccgg cgaccagcga ccagccgagc
     4081 caccggcgca gatcggcgcg cgaacggacc aggcgcccgt cggtcacgcc gccaaggtcc
     4141 cggcggttcc gctcgcgagc aagcggtcca gcgcgacgcg cgccgcggcg gtggtgggag
     4201 ccgtcacgag ctcctgcttg gcgtccttga cgagcgcgag gcgctcggcg taccaggggt
     4261 agaacttgcg caggtagtgg ccggcgcggt cggggccgag gtgctcctcg caacggtcga
     4321 tcgtccagcg cagctcgtcg atcacctcgg cggcggcggg ctcgccctca cgcagcccga
     4381 gcagccgctc gaacatccac ggattgccga gcgagccgcg ggcgagcatc acggcggcgg
     4441 cgcccgagcg ctcgaacgcc tcgacggcgc gctcgtcgga gagcaggccg ccggagacga
     4501 tcaccggcac gtcgagtgcc tcgacgacct cgcgcgcgag gtcgtagtca ggcttgcccc
     4561 tgtgctgctg cgacgcgtga cgcgggtgga acgagatcgc ggcgacgccc gcctcgtcga
     4621 ccagacggcg cgccaggtgg actccggcgc ggtcgcccgg gcgctggccg ggccgcaact
     4681 tcacagtgac cggcaggccg ctgccctccc ccgccgcctt ggcgatcgcc accgccttgt
     4741 cggggtcgtc gagcagggcc gcgcccgcgc cggtcttgca gaccttgcgg acgggacagc
     4801 ccatattgag gtcgacgagg tcggcgcccg cctcggcgac catcgcggcg gcctcgcgca
     4861 tcacgtcggc gtcgtggccg aagagctgca tcgagaccgg atgctcctcg gggtggatgc
     4921 gcaggaactg gtcgacggtg cgctcgttgc catagctcag gccgaagctc gacaccatct
     4981 ccgagacgac cagcaccgcc ccgtgtcgct tcgcctgcag ccgcacgaac cagttgccga
     5041 tccccgccag cggcgccagc acgagccggt tcggcagctc gatgccggcg agcgtccagg
     5101 gatccttcag ggagcgcaca agccccagct tacgggcggt ttctgtccca gatcagacgg
     5161 aggcccttca gggtcagcag gtcgtcgagc tcgtcgacct gctcgcagtg cgggacgatc
     5221 acgttcgccc agccacctgt ggcgatcgtc gtcgcctcgt cgccgagctc gtcgcgcagg
     5281 cgtgcgagca ggccgtctac ctgaccggcg tagccgtaga tcacacctga ctggatcgcc
     5341 tcgcgcgtgc ccttgccgat cgccgcgcgc ggcggttcga ggcggacctt cggcagcgcg
     5401 gcggcgcgct gggtgagcgc ctcgatcgag atctcgacgc cgggcgagat cacgccgccg
     5461 aggtactcgc cggcggccga gacgacgtcg tagttgagcg cggtgccgaa gtcgacggcg
     5521 atgcagggac caccgcagcg gtcgtaggcg gcgatcgcgt tgacgagccg gtcggcgccg
     5581 agctcgcgcg ggacgtcgat gcggatcggc atgccggtct tcagctccgg cccaaccagc
     5641 gctccctcgc cgcgcaggta gcggcgacgg acctcgtcgt actcctgctg caggctcggc
     5701 acgacgcacg agacgacgac cgcgtcgacg tcgcgcagcg agttctcgcg caggcgcagc
     5761 aggcctccga gcacgactgc gagctcgtcg gcggtcgccg tgcgcgaggt gtggaagcgc
     5821 cagtgctcca gcagctcctc gtcgcggaag agcccgacgt gcgtctgggt gttgccaacg
     5881 tcgaccgcga gcagcatccg gcgattattc catcgggggg ctcgcgcccc ccggaccccc
     5941 cgcattgctc tggctacgtg ctgtgctgtg gtcaggcggt cagaccgaga cgctcggcgc
     6001 gttggacggc ctcggcgcgg ttgcggacct cgagcttgcg gtagacgctg ctggtgtgct
     6061 ccttgacggt gtgcggcgag aggtggagct gggcggcgat ctcgcggttg gtggctccgg
     6121 tgacgagcag cccgagcacg tcgcgctcgc gcggcgagag cggggcgcgc ggctgctcct
     6181 tggcggcggc gaagacggtc cggccgttgc cgaccgcgcg caccgccgcg gcgacgtcgt
     6241 cggccgccca gtccttcgag acgaagccgg aggcgccggc cgcctttgcg gcctggggcg
     6301 agatccagcc tgcgccggag atcagcagca cgcgcgttgt cggcgactcg cggcgcaggc
     6361 cctcgcacat ctcggcgccc gactcctcgc cgacgaacag gtcgacgagc gcgacgtgcg
     6421 gctcgtaccg gcgcgccagc tcgcgcgcct cgtcgccgtt gcgggcggtc aggcagcgct
     6481 cgacccacgg ctgctcggtg agcagcaggc ggaagcccca atgaactacc tcgtggtcgt
     6541 cgacgatcag cactcgcagc cggcgccggt cgactgaggc accggtcatc cggattgccc
     6601 gtcggtcggg acgacgagac gcacgcgcca gtcgtggtga ccgtcgccgg tggggccgaa
     6661 gtcgaccact cccccactct gcagcgcgtc gagcgccgcg agacgcaggc ccatgccggc
     6721 gccgcgtccg cgccgtccac cgtcgacgcc gtcgttgacg acctcgagca cgaacgtccc
     6781 gtcgcgggcg ccgacacgca cggtcacgcg ggtcgggttc gcgtgcttgt gtgcgttgcg
     6841 caacgcctcg gccaggacgg actgggagag ctgctcgagc tcctgcggca cctcgaccgc
     6901 cggatcccac tcgacgtcga gcggcggccc gtcgtagctc gacccgagcc gctcgagctc
     6961 ctcgcgcagc gtcgcgccga tgtcggggcg cggcggcgcg accggtcggg cgagggcgtg
     7021 acggaggtct gcgaccgcgg cctggatctc gtcggcgcag cggcggcgct ggtcggcggt
     7081 cagctcggcc tcggcgccga gcaccagcga cacgccgaag aggcgctgca tcacccgctc
     7141 gtggacctca cgggcgaggt cgacccgctc cgacagcagc cgggctcgct catgctgggt
     7201 cgccgcgatg cgcgcactcg ccgcgagcgc cgcggtcttg ccgagcgtcc acatcgtgtg
     7261 ccgctcgcgc tccgtcagct cgaagcgcgg gccgccgcgg tcggcgaaga tcaccccgag
     7321 ccagcgaccg ccggccgaga ctggcgtgca tgtcatcgtc gtgatccccg gcagcgcggc
     7381 gtagcgcgcc ggcacttggc cgacgaggtc gccgtcggtt gcgacgacgc ggtcctcggc
     7441 gagcgcccgc tgcgcgatcg gcgtctcctc gagcgtcccg tggacgccgc cgaccagctc
     7501 cgggtcgaag ccgtgactgc ctgccggcac gactatccgg cgcgcctcct cgtagagcag
     7561 caggccggcg cgctccatcg acgtgagccg gcacatcgcc tcgcagagcc ggtcgtagaa
     7621 cgcgcggccg ccgctgccac cgtcggcgtc gaggccggcg agcagctcga ccatcgtgtc
     7681 gagcggctcg gtcggcgaca agctcgctga ggcctcgggc atgtcctagg tctacagcag
     7741 cgcgtcagcg gcggctggcg agatggagcc gcagcgcctg gtcgccggtc agcgacacga
     7801 cgcgctcgtc gccgcaggcg cggcagcggc agacgacctc gtggtcccac tcctcgacgc
     7861 gcagcgacca gtcgatctga ccggacaggc agacccggca ggccagcgac gcctcgacga
     7921 ggcacgcttg atcgtcgccg cggaagttgg ctgtctcggt ggactccatg cgtccagcat
     7981 tcccctccgc cgccgcgcca cccagccgat cccgcgcgat tccccccgtc tcggggggat
     8041 cgcctagtcg gtcgggtagg ggatagcgaa cggcccgtta gcgatccgct gcccacttcc
     8101 gccggggaac tccaggacca cgttggcccc cgatgggacc gtcgccgtgc cggtcagcgt
     8161 cgttccgctc gagggcgggt cgatcgcggc gaggacgaag atcgagagcc cgttgccgcc
     8221 accgggcgac gtgttgaagc tcatcgacct ggccccgagc ctcgccgagc cggtcgacgt
     8281 gacccagaag tcctggctct gccggaaggc cggcgtgttg aggttcgtca ggatgacgcc
     8341 gtcggtgtcg tagctgagcg acgtgttcga gaacgaaagc tcgacctgcg cgctcgcggg
     8401 catgcggacg gacacgatcg tcggcaccgg ctcggccgag ctcggatcga tgaataggct
     8461 cgcggagccg aagcgcttca ctcggctgcc gtcggagcgc ctgccgaagc ccttcagctg
     8521 gctgctgccg gtctggcgaa ggcgccggac ggcgaacgtc agcgtccgcc gcttcggcgc
     8581 gtagcgcggg cggcgcagct cgagcagcgc gatgtccctg ccggctggcg ccccatggac
     8641 gaccagcgcg gcgttcggcg gagcgccacg gaagctcgac cgccactgcc gcacgaaccg
     8701 ccggagcggc atcgtgccgc cggtgcgtcg cggacggtcg tcgaagcgca cggttcggcg
     8761 cggcacgtca cgcagcacga gccgcttggc gccgagtgcg ccggctcccg cctgctgtac
     8821 gtagagactc tcgccgacgg gcgcggcctg agctgaagcg gggaccgcga cgactaccgc
     8881 cgccgcgacg gcgggtacga ggaagctggg acgcgcgtag cgcaagctgg gctccttcga
     8941 agcagtgctg gacggcgcga gcgtatcgcg aggcgaatcg acgcgccgtt cggcggtcta
     9001 gagcgcttcc cggcgtcgca cgagctgatc ggcgaccgcg tcggcggcct ctgcgagccg
     9061 agtgccgtcg ggcagcgcga gatccggatc gagctccagt agcggctcga ggacgaagcg
     9121 gcgggcggtc acctcggggt gtggcagcgt gagccgctcc gagcggtggg tcaggccgcc
     9181 cagcagcagg acgtcgacgt cgatcggtcg cggtccgtgg cgtggaccgc ccggacggcg
     9241 tccgagcgcg cgctcgacgt ccttgcaggc gtcgagcaac tgctcgggat caaggtccgt
     9301 gtcgatcgcc aggcaggcgt tgaggaagtc cggctggtcg agcacctccc cttgcggcgc
     9361 cgtctcgtat accgcggacg acgcgacgac cgtgactcca cgccgctcga gctcgtcgcg
     9421 cgccgcgcgc agcgctccga gccggtcgcc ctcgttcgat ccgaggccga ggtagccacg
     9481 cacttcttag cccgtccgcg cgcagccggc agagccggcc gcttgcggct caggactcac
     9541 gccagacctc taccgatacc tcgtcgaccg gcagcgggat cggcggctcg ggcttggcgg
     9601 ccttgacgcg caccgagtcg gctccatagc ggctcatcag ccggtcggcg atcgccgcgc
     9661 agagccgctc gagcgtcttg taggagcgct cctgcgcggc gagcgcgacc tgctcgcaga
     9721 cctccgcgta gtcgacggtg tcctcgacgc gatcggtgac catcgcgtcg cagtcggcca
     9781 gctcgaacgc gacatcgatc accagccgct gccccagctc gcgctcggca tccgagacgc
     9841 cgtgctgcgt gtaaagcgac agccccgtga tctcgacggt gacggtcggc tccggaacct
     9901 cgtcgtcctc gtcgaagtcc cgctcgtcca agtcctcgtc gtcgggatcg atcggatcgc
     9961 ggtcgtcgat catctgcgct cctcaagcca gatcggcggc cggtccgccg gaccgtccga
    10021 ccgtagcagc ggccaccgcc aacgcgtcac ggaccggccc cacgtcgtgc acccgaaaca
    10081 ccgacgcccc ccgctccaac gccatcacat tcgtcgcgat cgtccccgcc aaccgctccc
    10141 ccgccggtcg ccccgtgatc tgcccgatga accccttccc cgacgtcccc accaccaccg
    10201 gccgcccgat cgcgcagatc tcatccagcc gccgcagcag ctccaagttg tgctccaccg
    10261 tcttcccgaa cccaatcccc ggatccagcc ataccttctc ctccgccacc ccctccccca
    10321 cagcgaacgc caaccgctgc tccaggaact cccgcacctc cgccaccacg tcgccatagc
    10381 gcgggtccgc ctgcatcgtc cgcggatcgc cctgcatgtg cacgaggcag cagctggctc
    10441 cccgatcggc caccaacccc gccagctccg gcgcgaagcg gaacgccgcc acgtcgttga
    10501 cgatcgtcgc ccccgcctcc agcgccgcct cggcgaccgc cagcttcgac gtatcgatcg
    10561 agattcgcgc tgcacccgcc gccgcgagcc gctccaccac cggcgcaaca cgctccaact
    10621 cgaccgcctc cgacaccggc tcagcccccg gccgcgtcga ctccccaccc acgtccagga
    10681 tctccgcccc ctcgtcggcc aactcctctc catgagcaac cgccgcgtcg gcgtcgatgt
    10741 acagcccccc atccgagaac gaatccggcg tcacattcac cacgcccatc acggagtaag
    10801 caggggtcac atccgaatta tcgagcaccg gccatcgccc gcctccgcgg gtggcgacga
    10861 tttgcgttcg cgcgcgcagc ttgcgagcac gggctctccc ggttcacgac gcgagcttgc
    10921 gagcggtgca ctccaagaga caggacccgc gggcagcaac gcctgcagga aagccggctc
    10981 agtcagcagt gccgccggca agccccggcc gcggcaaagg cttcggcgcc cgctccggct
    11041 tccgcgccgc cggctcctcc accggctccg ccggcgtccc cgacggcagc accggctcgt
    11101 ccggcccgaa cacctcgagc tccgtcttcc cctcgagcag ctgaatgaac tcctcgcgct
    11161 cgatcgtctc ccgccgcagc aggatctccg acgtatgcga gaggtcgtca cgatgctgct
    11221 ccaagatgtt ccgcgccgac tggtgcgcct cctcgacgac ccgccggatc tcgtcgtcga
    11281 tctcgcgcgc cacgtcgtcg gagtagtccg gctccgacga gtactcacgc ccgaggaacg
    11341 gctgcccgtg gtcgtggccg aacacgcgcg gcccgagctt ctccgacatc ccgaagcgca
    11401 tgaccatctg cttcgcggtc gccgtcacct tctcgatgtc gttcgacgcc ccggtggtca
    11461 cctcaccgaa cacgagctcc tcggcggcgc ggccgccgag cgtcatcgcc atcgtgtcct
    11521 gcagctcggc gcgcgtagtg aggaacttgt cctcggtcgg cagcgagatc gtgtagccga
    11581 gcgcttggcc gcgcgagatg accgagatct tgtggaccgg gtcggtgttc tcgaggaagt
    11641 gggcgacgag cgcgtggccc atctcgtggt aggcggtgat gcgccgctcc ttctcgctca
    11701 tcacgcgcgt cttcttctcc ggcccggcga tcacgcgcat gatcccctcc tcgagctgga
    11761 ggtggtcgat ctcgcgcttg ccctggcggg cagacaggag ggccgcctcg ttgacgaggt
    11821 tctgcaggtc ggcgccggtg aagccgggcg tctgggcggc gaggttgtcg aggtcgatgt
    11881 gcttggcgag cggcttgccg cgcgtgtgga cctcgaggat cttcgcgcgg cccttgcggt
    11941 cggggcggtc gacgacgatc tggcggtcga agcggccggg gcgcagcagc gccgggtcga
    12001 ggatgtcggg ccggttggtg gcggcgatca ggatgatgtt gtcggtcatc gtgaagccgt
    12061 ccatctcgac gagcagctgg ttgagcgtct gctcgcgctc gtcgtggccg ccgcccatgc
    12121 cggcgccgcg atggcggccg acggcgtcga tctcgtccat gaagatgatg caggggctgt
    12181 tctgcttcgc ctgctcgaag aggtcgcg
//
LOCUS       DATABV010000107         5626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2120369, whole genome shotgun sequence.
ACCESSION   DATABV010000107 DATABV010000000
VERSION     DATABV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120369"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1086)
                     /locus_tag="VHF88_04540"
     CDS             complement(13..1086)
                     /locus_tag="VHF88_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX2161073.1"
                     /translation="MRVAVLGVGLIGGSIGLAARKCVQGSEVVGFGRSRERLDRAREL
                     GAIDRAAGSIEEALDGAAICFCCAPVGALGEQIDAALAAADDDCVVSDVGSIKGGLVE
                     AAADRRFVGGHPIAGAETAGVDHARPELFQGATWYLTPTADTSGVLFERLHRFIAALG
                     ARPVAIDADSHDRLLATVSHLPHVLANTLVTQAAGALGDQDAGLPRVGPSFRDATRVA
                     GANSAIWTDIYVANRDAIAAEVDAVVERLREAADLLRTGEPEPIAAWNERAAEARRQL
                     LEADLAGGVVHELRTTVPNRPGIVAEVALALGKAGVNIVDMALAPAPDMRSGAITLWI
                     AGDDQAERARGLIDDLGFPVAEA"
     gene            complement(1083..2156)
                     /locus_tag="VHF88_04545"
     CDS             complement(1083..2156)
                     /locus_tag="VHF88_04545"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX2161074.1"
                     /translation="MTEGVTTGRDHVRDQRIERAVPLVTPAQLLAEQPLGAERERTVI
                     AGREQVAAVLDGHDDRLLVVVGPCSVHDTEAALEYARRLAEVAASRRDDLLIAMRVYF
                     EKPRTTTGWKGLINDPHLDGSVDVNAGLHMARALLLEVLSLGLPIGVEFLDPITPQYI
                     SDTVAWGAIGARTTESQIHRQLGSGLSMPIGFKNRTDGDVQVAVDAVRAAAVSHAFAG
                     IDDSGTPAILRTTGNPDCHVILRGGSGAPNYGAAHVEQALAKLRAAGLPERLVIDASH
                     DNSGKDPDRQPVAAEDIGAQVAAGNRAIVGVMLESFLVAGRQGLGGELTYGQSITDAC
                     MGWDQTAAVLDRLAAAVRVRRDG"
     gene            complement(2426..3499)
                     /gene="hisC"
                     /locus_tag="VHF88_04550"
     CDS             complement(2426..3499)
                     /gene="hisC"
                     /locus_tag="VHF88_04550"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX2161075.1"
                     /translation="MSIEFNSRIERIPVYPAAKTWEHEGELVELASNETPWGPLPAVA
                     GALQGQIERLNRYPDPGKTLLRQRIADRSGMPIARVAVGNGSCEILISAADALLEPGS
                     ELIYAWPSFSIYPHIAAQSGARAIEVPLDADQRHDLAAMAREVTAATRLVLICNPNNP
                     TATALPVEAIDGFMADLPRHVAVILDEAYVEFNALQDPDDSLDLVARHPNLIVLRTFS
                     KVYGLCGLRAGYALGPEEFRLAVDRVRQPFSVNALAQAAAAEAIRHQDEVARRVEATV
                     VERAWLEVELEDRGLPTTDSQANFVWVGLGEREEAPIVDGLAQRGVIVRSGAALGRGG
                     NLRVTLGTRHQNERFVAALDELL"
     gene            complement(3496..3861)
                     /gene="aroH"
                     /locus_tag="VHF88_04555"
     CDS             complement(3496..3861)
                     /gene="aroH"
                     /locus_tag="VHF88_04555"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX2161076.1"
                     /translation="MRLRALRGATTVDENTAEAILGATDELLRALIERNELAGDEMVS
                     CIFTCTDDLDAEFPAVAARRLGLSSVPLLCAREIDVPGALPRVIRVLLHTYADPQAEP
                     RHVYLRDAVGLRADLEGAQ"
     gene            complement(3858..4553)
                     /locus_tag="VHF88_04560"
     CDS             complement(3858..4553)
                     /locus_tag="VHF88_04560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX2161077.1"
                     /translation="MRLAKYLAHAGVASRRAAEGIVADGRVTVGGKVVTDPARDVDDS
                     SDVAVDGKPIAPEPHETYVLNKPAGVVSTAQDTHGRPTVIDLVCSDRRLYPVGRLDAD
                     TTGLILLTNDGALAERLTHPRYGVEKVYRVEVDPPRPSERALRALREGVELDDGRTAP
                     ARAHLVEPGVIEIAIHEGRNRQVRRMLEAVGHRVTGLRRTAFGPLRLGRLGVGDARRL
                     TPAEIERLKQSAQ"
     gene            4587..5612
                     /gene="rlmN"
                     /locus_tag="VHF88_04565"
     CDS             4587..5612
                     /gene="rlmN"
                     /locus_tag="VHF88_04565"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX2161078.1"
                     /translation="MDLERLDSLLAERGQPAFRAAQVWEWLARGAASYDEMTNLPAAL
                     RETLAAELPLSTLALETEAHSRDGTEKALFQTGDGRPLETVLMRYRDGRRSLCLSSQS
                     GCPLTCTFCATGTMKFGRNLTASEILDQALHFRRKEEVDHAVFMGMGEPLLNLDNVLA
                     ACERLPDVGIHPSSTTISTVGWIPGIERLTERDGPTIRLALSLHAPDDALRSELMPVN
                     DRYPLADVLDACRAWSEARRRKVFVEYLMLDGVNDRYEQAVALADLLQPRNAFKVNLI
                     PYNPTEAGFQGSGRDAIAAFRAALEQRGVRTTVRLTRGRDIDAACGQLAARASVDGEP
                     RAAHDQD"
BASE COUNT          759 a         1994 c         2100 g          773 t
ORIGIN      
        1 agcgcgcgcc gctcacgcct cggcgacggg gaagccgagg tcgtcgatca gcccacgggc
       61 gcgctcggcc tggtcgtcac cggcgatcca gagcgttatc gcacccgagc gcatgtcggg
      121 agccggcgcg agcgccatgt ccacgatgtt cacccctgcc ttgccgagcg ccagcgccac
      181 ctcggcgacg atacccggcc ggttggggac ggtcgtgcgc aactcgtgaa cgacgccgcc
      241 ggcgaggtcg gcctcgagca gttgccgccg cgcctccgcg gcacgctcgt tccaggcggc
      301 gattggctcc ggctcgcccg tccgcagcag atcggccgcc tcgcgcaggc gctcgacgac
      361 agcgtcaact tcggcggcga tcgcgtcgcg gttcgcgacg tatatgtcgg tccaaatcgc
      421 cgagttggcg cccgcgaccc gggtggcgtc gcggaagctc gggccgacac ggggcaagcc
      481 ggcgtcctgg tcgccgagcg cccccgccgc ctgggtcacg agcgtgttgg cgagcacgtg
      541 cggcaggtgg ctgacagtcg ccagcaggcg gtcgtgggag tcggcgtcga tcgcgacagg
      601 ccgcgcaccg agggccgcta tgaagcggtg cagccgctcg aacagcacgc cggaggtatc
      661 ggcggtcggc gtcaggtacc aggtggctcc ctggaagagc tcgggacggg cgtggtcgac
      721 gcccgccgtc tcggcgccgg cgatcggatg gccgccgacg aagcgccggt cagcggcagc
      781 ctcgaccagc ccgcccttga tcgagccgac gtcgctgacc acgcagtcgt cgtcggcggc
      841 tgccagggcc gcgtcgatct gctcgccgag ggccccgacc ggggcgcagc agaagcagat
      901 cgccgcgccg tcgagcgcct cctcgatcga tccggcggca cggtcgatcg cgcccagctc
      961 gcgggcgcga tcgaggcgct cgcggctgcg cccgaagccg accacctcgg agccttggac
     1021 gcacttccgc gcggcgaggc cgatcgagcc gccgatcagc ccgacgccga ggacggcgac
     1081 cctcatccgt cgcgacgcac ccgcaccgcc gccgccaggc ggtcgagcac ggcggcggtc
     1141 tggtcccagc ccatgcaggc gtcggtgatc gactggccgt aggtcagctc accgccgagc
     1201 ccctgacgac cggcgacgag gaacgactcg agcatcactc cgacgatcgc gcggttgccg
     1261 gcggcgacct gggcaccgat gtcctcggca gccaccggct ggcggtcggg gtccttgccg
     1321 ctgttgtcgt gcgaggcgtc gatcacgagc cgctccggca acccggccgc acgcagcttc
     1381 gcgagcgcct gctcgacgtg ggcggcgccg tagttcgggg cgccgctgcc gccgcgcagg
     1441 atcacgtggc agtcggggtt gccggtggtg cgcaggatcg ccggcgtgcc gctgtcgtcg
     1501 atcccggcga aggcgtgcga cacggcggcc gcgcgaacgg cgtcgacagc cacctggacg
     1561 tcgccgtcgg tgcggttctt gaagccgatc ggcatcgaca ggccggagcc gagctggcgg
     1621 tggatctggc tctcggtcgt gcgcgcgccg atcgcgcccc aggcgacggt gtcggagatg
     1681 tactgcgggg tgatcgggtc gaggaactcg acgccgatcg gcagcccgag cgagagcacc
     1741 tcgagcagca gcgcgcgcgc catgtgcagg ccggcgttga cgtcgaccga gccgtcgagg
     1801 tgcgggtcgt tgatcagccc cttccaaccg gtcgtcgtgc gcggcttctc gaagtagacg
     1861 cgcatcgcga tcagcagatc atcgcggcgg ctcgcggcaa cctcggcgag ccgccgggcg
     1921 tactcgagcg ccgcctcggt gtcgtggacg ctgcacggcc cgacgacgac cagcagcctg
     1981 tcgtcgtggc cgtcgagcac cgcggcgacc tgctcgcgcc cggcgatcac ggtccgctcg
     2041 cgctcggcgc cgagcggctg ctcggcgagc agctgcgcgg gcgtaacgag cggcacggcg
     2101 cgctcgatgc gctggtcgcg gacgtggtcg cggccggttg tcaccccctc tgtcattccg
     2161 atcatcatgt cgcttctcca gctccttcga agtcctgggc cgcctggcgg gcggcggatg
     2221 cacgggcgtg ggctatcggc gtccgactgg gaccgctcgg ccgacggcag gggctgccgc
     2281 tgccggacgc ctagctaaat cgccaatagg actcgcaggc acgccgaagg acgcccggga
     2341 gggcggctgt gctggtcggc gtggcggtgc gggccatcga gctacttgta gcaggcggcg
     2401 caaggcgcgg caagcggccc gctcgctaga ggagctcgtc cagcgccgcg acgaagcgct
     2461 cgttctgatg gcgggtcccg agcgtcacgc gcaggttgcc ccccctcccg agtgctgcac
     2521 cggagcggac gatcacgccg cgctgggcga gcccgtcgac gatcggcgcc tcctcgcgct
     2581 cgccgagccc aacccagacg aagttcgcct ggctgtcggt ggtaggcagc ccgcggtcct
     2641 cgagctcgac ctcgagccat gcccgctcga cgaccgtcgc ctcgacacgg cgcgcgacct
     2701 cgtcctgatg gcggatcgcc tcggcggcgg ccgcctgggc gagcgcgttg accgagaacg
     2761 gctggcggac gcggtcgacg gcgagccgga actcctcggg gccgagcgca tagccggcgc
     2821 gcaggccgca gaggccgtag accttggaga aggtgcgcag gacgatcagg ttcggatgcc
     2881 gcgcgaccag gtcgagcgag tcgtcggggt cctggagcgc gttgaactcg acgtaggcct
     2941 cgtcgaggat caccgcgacg tggcgcggca ggtccgccat gaacccgtcg atcgcctcga
     3001 ccggcagcgc tgtcgcggtc ggattgttcg ggttgcaaat caggaccagg cgcgtcgcgg
     3061 cggtgacctc gcgcgccatc gcggcgaggt cgtggcgctg gtcggcgtcg agcgggacct
     3121 cgatcgctcg ggcgccggat tgggccgcga tgtgcgggta gatcgagaac gacggccagg
     3181 cgtagatcag ctccgagccc ggctcgagca acgcgtcggc ggccgatatc aggatctcgc
     3241 aggagccgtt tccgacggcg acgcgcgcga tcggcatgcc cgagcggtcg gcgatccgct
     3301 ggcgcagcag ggtcttgccg gggtcggggt agcggttgag ccgctcgatc tggccttgaa
     3361 gcgcgccggc gaccgccggc aacggtcccc agggcgtctc gttcgaggcc agctcgacca
     3421 gctcgccctc gtgctcccag gtcttcgccg cggggtagac ggggatccgc tcgatccgcg
     3481 agttgaactc gatgctcact gggcgccctc gaggtcggcg cgcaggccga cggcgtcgcg
     3541 caggtagacg tggcgcggct cggcctgcgg gtcggcgtag gtgtgcagca gcacgcggat
     3601 cacgcgcggc agcgcgccgg ggacgtcgat ctcacgcgcg cagagcagcg gcacgctcga
     3661 cagcccgagc cgccgggcgg cgacggccgg gaactcggcg tcgaggtcgt cggtgcaggt
     3721 gaagatgcag ctgaccatct cgtcgccggc gagttcgttg cgctcgatca gcgcgcgcag
     3781 cagctcgtcg gtcgcgccga ggatcgcctc ggcggtgttc tcgtccacgg tggtggcgcc
     3841 ccggagcgct cgcagcctca ttgcgccgat tgtttcagcc gctcgatctc ggccggcgtg
     3901 aggcggcgcg cgtcgccgac gccgaggcga ccgaggcgca gcgggccgaa ggcggtgcgg
     3961 cgaaggccgg tgacgcggtg gccgacggcc tcgagcatcc gccgcacctg gcggttgcgg
     4021 ccctcgtgga tggcaatctc gatcacgccg ggctcgacga ggtgggcgcg cgccggcgcg
     4081 gtgcggccgt cgtcgagctc gacgccctcg cgcagcgcgc gcagcgcccg ctcggacggt
     4141 cgcggcgggt cgacctcgac gcgatagacc ttctcgacgc cgtagcgggg gtgggtcagc
     4201 cgctcggcga gcgcgccgtc gttggtcagc aggatcaggc cggtggtgtc ggcgtcgagc
     4261 cggccgaccg ggtagaggcg gcggtcggag cagacgagat cgatcacggt cggccggccg
     4321 tgcgtgtcct gggccgtcga gacgacgccg gcgggcttgt tgaggacgta ggtctcgtgc
     4381 ggctcgggcg cgatcggctt gccgtcgacc gcgacgtcgc tgctgtcgtc gacgtcgcgc
     4441 gccggatcgg tgacgacctt gccgccgacc gtcacgcggc cgtcggccac gatcccctcg
     4501 gcggcgcggc gcgaggcgac gccggcgtgg gcgaggtact tggcgagccg catcgggcaa
     4561 ggctcgcagg ctgcgagact tgccgcgtgg acctcgaacg gctcgacagc ctgctcgccg
     4621 agcgcgggca gccggcattc cgcgccgcgc aggtgtggga gtggctggcg cgcggcgctg
     4681 cgtcatatga cgagatgacg aacctgccgg ccgcgctgcg cgagacgctc gccgcggagc
     4741 tgccgctatc gacgctggcg ctcgaaaccg aggcccactc gcgcgacgga accgagaagg
     4801 cgctcttcca gaccggcgac ggccggccgc tcgagactgt gctgatgcgc taccgcgacg
     4861 gccgccgctc gctctgcctc tcgagtcagt cgggctgccc gctgacctgc accttctgcg
     4921 cgaccgggac gatgaagttc ggccgcaacc tgacggcgtc ggagatcctc gaccaggcat
     4981 tgcacttccg ccgcaaggag gaggtcgacc acgccgtctt catggggatg ggcgagccgc
     5041 tgctgaacct cgacaacgtc ctcgccgcct gcgagcggct gcccgacgtc ggcatccacc
     5101 cgtcgagcac gacgatctcg acggtcggct ggatcccagg gatcgagcgg ctgaccgagc
     5161 gcgacgggcc gacgatccgc ctcgcgctgt cgctgcacgc gcccgacgac gcgctgcgct
     5221 cggagctgat gccggtcaac gaccgctacc cgctcgccga cgtgctcgac gcctgccgcg
     5281 cgtggagcga ggcgcgcagg cgcaaggtct tcgtcgagta cctgatgctc gacggagtca
     5341 acgaccgcta cgagcaggcg gtcgcgctcg cggaccttct gcagccccgc aacgccttca
     5401 aggtcaacct catcccctac aacccgaccg aagcggggtt ccagggctca ggccgtgacg
     5461 cgatcgccgc gttccgcgcc gcgctcgagc agcgcggggt ccggacgacc gtccgcctga
     5521 cccgcggccg cgacatcgat gccgcctgcg gccagctcgc cgcgcgtgcc tccgttgacg
     5581 gcgagccacg agccgcccac gatcaggact aggccggcca gcgtgg
//
LOCUS       DATABV010000108         5877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2682889, whole genome shotgun sequence.
ACCESSION   DATABV010000108 DATABV010000000
VERSION     DATABV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2682889"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VHF88_04570"
     CDS             complement(<1..743)
                     /locus_tag="VHF88_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161079.1"
                     /translation="MASRWRRTALDGGRRMPAAASPTGFDGRRRTAAGALTITLGCLL
                     AFALLAPAGADAACGKVKRFAARADAGLDRAPLAIGDSVLLGAAEEAAAVGYDVDVRG
                     CRQIDEGLDVLRARARRGALPRVVVIALGSNASIALADVRTALRILGPKRTLGLVTPR
                     EIRGMPLDDAATLRRAAARWSRRVVLVDWVAQTRRRRGLTYSDGIHLTPAGQRMLATM
                     LKAPLERVAPGAGTPPPAGGGTAGGGAQAP"
     gene            complement(1324..2847)
                     /locus_tag="VHF88_04575"
     CDS             complement(1324..2847)
                     /locus_tag="VHF88_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2161080.1"
                     /translation="MSNDQYVLTLCPAAGGDLDLTSLRCRAIADAHVRFRRAVGDEVA
                     LVHAGGGDDDPSGGLAIGGGAARRVDPAVERWAQWLLERLDGAGLIYHREGKGWRLRS
                     GKLHEESERRLEELSGWSDAALAGQRKLLEHVDDPGDSGSELEQSLSKLAAAGWSVDS
                     KRDKGPPTVHFAAGDMPLAEGDDWRAAGGVHPRLAAALSALVGALSSDEREAAPSDAV
                     SGLGRRLPATTVAAPGSDSDVALLDLRTVAKALRDVGGLDLPGGEPLGPVLAAGAFQL
                     LPVRRLPEQGAPASNPEPTGPSLNGTAPAAATAALNSAEGDASVTPTGGQPPADSNDV
                     ATLIAVHGADAVRFALLHAAAPAKRFRGGEDVVGYAARFLTELRELAASRWGDAPPAA
                     RIETDDGLRRRLAGWCDTALARTTENHARLDMHRATRNAVELLARIKDFEARVTEQRG
                     EVAGADREAVAVALALLVQLLAPLAPATADELWRQSRRDGSAGDAPWPVLQRATAAA"
     gene            complement(2890..3082)
                     /locus_tag="VHF88_04580"
     tRNA            complement(2890..3082)
                     /locus_tag="VHF88_04580"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2926..2928),aa:Glu,seq:ttc)
     gene            complement(3138..3722)
                     /locus_tag="VHF88_04585"
     CDS             complement(3138..3722)
                     /locus_tag="VHF88_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161081.1"
                     /translation="MPKIMIVDNGSLSTDVLAARLEGLGAETTIVSLGELSPDMAEEG
                     GFDGVVLTGTDVPSTTPYVYETELELIRRTEVPLLGICGGMHLIGKAHGVGIGDCKHA
                     VGKTPVHVDGDEALFEQLESPALLFERHRYQLMDVPEGFRRIAWGDHCHTEGIRHAER
                     PTYGLQAHVEFRPEGKVILRNFVSLTGLDSAVAA"
     gene            complement(3759..4583)
                     /gene="dapF"
                     /locus_tag="VHF88_04590"
     CDS             complement(3759..4583)
                     /gene="dapF"
                     /locus_tag="VHF88_04590"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX2161082.1"
                     /translation="MSDDRLEFRKYYAEGNSYVVIEAQPGAASLAGWIADDTRGLGGD
                     GLILIEGSPGEPMHHMRVFNADGSEAQWCGNGARAAAALIAERDDLREGESVTITSGG
                     GTAQHVLLDRETWTFRAEMPVPADPVVEVGEDRRLVQMLIGAPHLVVFGPRPTGEQLD
                     DAGAALCAARPGGTNVMFASRDDDGGLTVTPWERGVGPVLGCATGAAAAAIAAERVLG
                     RPLNGGRVRQPGGAIDVRWDGGERVLEMTGTARLVATGTVALDRAERPMAGVSSSG"
     gene            complement(4580..>5877)
                     /locus_tag="VHF88_04595"
     CDS             complement(4580..>5877)
                     /locus_tag="VHF88_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2161083.1"
                     /translation="RDRRGVGHRGQRSRRPGRRERQRAGSRRMKLAVFRQEEFFEDYE
                     FSCELMLALSGAEPMTAAELTEIVGEPPAPDWEVGYTPSSGYPELRDAIAALNPPAAR
                     ENVMVTVGAIEALLILHNLLLEPGDEVVCLWPAYQPLYELARGAGASVRFVELEHTDG
                     FAIDLGAIEDAVGPRTKVVLLNVPHNPTGQTVAPDDLRELARRLAPLGVHLVVDEVFR
                     EMRSHHPPSAWDGQDNLVVIGSMSKSYALPGLRIGWLVANPELVVKARQFRKYTSLNP
                     GSRDQQWALAALEHRDAVLARTWALTEDGAALATSWLDEHRDDFELTAPPAGGLFFPR
                     LRHDVPTLDFCAELVHDTGVLLAPGSVCYESEGFLRLGVATPQLAAGLERLDAWLDDR
                     RGRTAELAGGASTADRAGAAAAAATRPADLASRNGSRPL"
BASE COUNT          766 a         2191 c         2112 g          808 t
ORIGIN      
        1 ggcgcctggg cgccgccgcc agccgtgccg ccgcccgcgg gcggcggcgt ccccgcgcct
       61 ggcgcgacac gctcgagcgg cgccttcagc atcgtcgcga gcatgcgctg gcccgcgggc
      121 gtcaggtgga tcccatcgga gtaggtgaga ccgcgccgcc ggcgcgtctg cgcgacccag
      181 tcgacgagca cgacgcgccg cgaccagcgt gccgccgccc tgcgcagcgt cgcggcgtcg
      241 tcgagcggca tgccgcggat ctcgcgcggc gtgacgagtc cgagcgtccg cttaggcccg
      301 aggatccgca gcgcggtgcg cacatcggcg agcgcgatcg aggcgttcga cccgagcgcg
      361 atcaccacca cgcgcgggag cgccccacgg cgggcccgcg cgcgcaacac gtcgagcccc
      421 tcgtcgatct ggcggcagcc gcgcacgtcg acgtcgtagc cgaccgccgc ggcctcctcg
      481 gccgcgccga gcaggaccga gtcgccgatc gccagcggcg ctcgatcgag gccggcgtcg
      541 gcgcgcgcgg cgaagcgctt gaccttgccg caggccgcgt cggccccggc tggcgctagc
      601 agcgcgaagg ccagcaggca gccgagcgtg atcgtcagcg ctccagccgc tgtccgccgc
      661 ctgccgtcga agcctgtcgg ggatgctgca gcgggcatcc gccggccgcc gtcgagcgcc
      721 gttcgtcgcc accgagaagc caccgtggca ctgtggcaga agccgcggcc gcccgcctcg
      781 attgaaacgc ggcgaggcac ttgacgagga aggcggctct cggtaagttc gtgtctgcaa
      841 tccggggagg gttccggtca ttgctccccc gaaggaggga aggggaagtg gagaaggtct
      901 gcgcagcttg ggtatacgga cgcggtcgcg gcgccgagcc gcggtgtgaa aggccgccga
      961 tcccgaatgc cttcgacagc gcgcgggccg gctgtgtcgt cgcgccgagg cctcgatctc
     1021 gcccggggtc tcggcgccca tagcgacaca ggtggggcgg ctctcggcgc cgtcccttca
     1081 cgaaaatttt cctcgcttgt aagacatagg gccccggcac gcgccggggc cctatgcgtt
     1141 cccctgccca cagtcgccgc ccgtcctgaa taggcgaccg cgtggcactc ccattccctc
     1201 ccctaagacc tcgggaacgg gataaggacg cgaccttacc cggcacttgc gaccctgaca
     1261 agggccatcc gggtgcgcta tcggaaccgg tttcgcgcgt ctggccgggg ccccggcggt
     1321 ggttcaggcc gcggcggtcg cgcgctgcaa gaccggccac ggcgcgtcgc ctgctgagcc
     1381 gtcccggcgg gactgccgcc agagctcgtc cgcggtcgcc ggggcaagcg gggcgagcag
     1441 ttggacgagg agcgccagcg cgacggcaac ggcctctcgg tcggcgccgg cgacctcgcc
     1501 gcgctgctcg gtgacgcgcg cctcgaagtc cttgatccgg gccagcagct cgaccgcgtt
     1561 gcgggtcgcg cgatgcatgt cgagccgcgc gtggttctcg gtggtccggg ctagggcggt
     1621 atcgcaccag ccggcgagcc ggcggcgcag cccgtcgtcg gtctcgatgc gggcggcggg
     1681 cggcgcatcg ccccagcgcg acgccgcaag ctcgcgcagc tccgtgagga accgcgccgc
     1741 gtagccgacg acgtcctcgc caccccggaa gcgcttcgcg ggcgccgcgg cgtgcagcag
     1801 ggcgaagcgc acggcgtccg cgccatggac ggcgatcagc gtggcgacgt cgttggagtc
     1861 ggctggcggc tggccgcctg tgggcgtgac gctggcgtcg ccctcggcgg agttgagggc
     1921 cgccgtcgct gcagcgggcg ccgtcccgtt caagctgggt cctgtcggct cgggattact
     1981 cgcgggagcg ccctgctccg gcaggcgccg aactgggagc aactggaacg ctcccgccgc
     2041 caggaccggc ccgagcggct cgccgcccgg caggtcgagg ccgccgacgt cgcgcagcgc
     2101 cttggcgacg gtgcgcaggt cgagcagcgc gacatcgctg tcggagcccg gcgcggcgac
     2161 cgtcgtcgcc ggcagccggc ggccgaggcc actgactgca tcggacggcg ccgcctcgcg
     2221 ctcgtcggac gacagcgctc cgaccagcgc gctcagcgcc gccgcgaggc gcggatgcac
     2281 gccacccgcg gcgcgccagt cgtcgccctc ggcaagcggc atgtcgccgg cggcgaagtg
     2341 gactgtcggc gggcccttgt ccctcttcga gtcgaccgac cagccggccg ccgcgagctt
     2401 cgacaggctc tgctcgagct cactgccgga gtcgcccggg tcgtcgacgt gctcgagcag
     2461 cttgcgctgg ccggcgagcg ccgcgtcgct ccagccgctc agctcctcca gccgccgctc
     2521 gctctcctcg tgcagcttcc cgctgcgcag ccgccagccc ttgccctcgc ggtggtagat
     2581 cagccccgcg ccgtcgagcc gctcgagcag ccactgcgcc cagcgctcga ccgccgggtc
     2641 gacacgccgc gcggccccgc cgccgatcgc caagccgcct gacgggtcgt cgtcgccgcc
     2701 cccagcgtgg acgagcgcca cctcgtcgcc gacagcacgg cgaaagcgca cgtgcgcatc
     2761 ggcgatcgca cggcagcgca ggctcgtgag gtcgaggtcg ccgccggcgg cgggacagag
     2821 agtgagtacg tattggtcgt ttgacacgtg aggcgatccg gtcaccggca gacccggctt
     2881 tttggtgagt acgcccgacg ggactcgaac ccgtatctgc ggcttgaaag gccgctgctc
     2941 ttagataggc cggattgggt atccgggtgt tcgaacggcc tgtgaaggca tcccgccacc
     3001 gacacgaagt cggctagaag gctggcattc gagacccggg taaccccagg gactacccat
     3061 gtaccgattg agctacggac gcatgattgg tgctacggaa ctccctcctg ttccttccct
     3121 ccgtatggcc acactaccta agcggccacc gccgagtcaa ggcccgtcag cgacacgaag
     3181 ttgcgcaaaa tgaccttgcc ctccggcctg aactcgacat gcgcctggag gccgtaggtc
     3241 ggccgctcgg cgtgacggat tccctcggtg tggcagtggt cgccccaggc gatccgccgg
     3301 aagccctcgg gcacgtccat cagctggtag cgatggcgct cgaacagcag cgccgggctc
     3361 tcgagctgct cgaagagcgc ctcgtcgccg tccacgtgga ccggcgtctt gccgactgcg
     3421 tgcttgcagt ccccgatgcc gacgccgtgc gccttgccga tcaggtgcat cccgccgcag
     3481 atgccgagca gcggcacctc ggtgcgacgg atcagctcga gctcggtctc gtagacgtac
     3541 ggcgtcgtcg acgggacgtc ggtgccggtg agcaccaccc cgtcgaagcc gccctcctcg
     3601 gccatgtccg gcgagagctc gccgagcgag acgatcgtcg tctccgcccc gaggccctcc
     3661 agacgggccg ccagtacgtc cgtcgaaagg ctcccgttgt cgacgatcat gatcttcggc
     3721 acttgtgctc ccctcctgag caacttgctt tccgtttcct agccggagct gctgaccccg
     3781 gccatcggtc tctccgcccg gtccagtgcg accgtcccgg tcgccaccag ccgagccgtg
     3841 cccgtcatct cgagcacccg ctcgcctccg tcccagcgga cgtcgatcgc gccgcccggc
     3901 tggcgcacgc gcccgccgtt cagcggccgc ccgagcaccc gctcggcggc gatcgccgcc
     3961 gccgcggcgc cggtcgcgca acccagcacc ggcccgacgc cgcgctccca cggcgtcacg
     4021 gtcaggccgc cgtcgtcgtc gcgcgaggcg aacatcacgt tggtcccgcc cggacgcgct
     4081 gcgcagagcg ccgcgccggc gtcgtcgagc tgctcgccgg tcgggcgcgg cccgaagacg
     4141 acgaggtgcg gcgcgccgat cagcatctgc accagccggc ggtcctcgcc gacctcgacg
     4201 accgggtcgg ccggcaccgg catctcggcg cggaaggtcc aggtctcgcg gtcgagcagc
     4261 acgtgctgcg cggtgccgcc gccgctggtg atcgtgacgg actcgccctc gcgcaggtcg
     4321 tcgcgctcgg cgatcagggc cgccgccgcg cgggcgccgt tgccgcacca ctgcgcctcc
     4381 gagccgtcgg cgttgaagac gcgcatgtgg tgcatcggct cgcccggcga gccctcgatc
     4441 aggatcagcc cgtcgccgcc gagcccgcgc gtgtcgtcgg cgatccagcc ggcgagcgac
     4501 gccgcgcccg gctgcgcctc gatcaccacg tacgagttgc cctcggcgta gtacttgcgg
     4561 aactcgagcc ggtcgtcgct cacagtggcc tcgatccgtt gcgcgacgcg aggtcggcgg
     4621 gccgggtggc ggcggccgca gccgcgcctg cccggtccgc cgtgctcgcc cctcccgcca
     4681 gctccgctgt gcggccgcgt cggtcgtcga gccacgcgtc gagtcgctcc aggccggccg
     4741 ccagctgcgg cgtagcgacg ccgagccgca ggaagccctc gctctcgtag cagacgctgc
     4801 ctggcgcgag caggacaccg gtgtcgtgca ccagctctgc acagaagtcg agcgtcggca
     4861 cgtcgtgacg caggcgcggg aagaagaggc cgccggccgg cggcgcggtc agctcgaagt
     4921 cgtcgcggtg ctcgtcgagc cagctcgtcg ccagcgccgc gccgtcctcg gtcagcgccc
     4981 aggtccgcgc gagcacggcg tcgcggtgct cgagcgccgc cagcgcccac tgctggtcgc
     5041 gcgagccggg gttcagcgac gtgtacttgc ggaactggcg cgccttgacg acgagctccg
     5101 ggttggcgac cagccagccg atccgcagtc cgggcagcgc gtaggacttg ctcatcgagc
     5161 cgatcacgac caggttgtcc tggccgtccc aggccgacgg cgggtggtgc gagcgcatct
     5221 cgcggaagac ctcgtcgacg acgaggtgga cgccgagcgg cgccagccgg cgcgcgagct
     5281 cgcgcaggtc gtcgggcgcg acggtctggc ccgttgggtt gtgcgggacg ttgagcagca
     5341 cgaccttcgt gcgcggcccg acggcgtcct cgatcgcgcc gaggtcgatc gcgaagccgt
     5401 cggtgtgctc gagctcgacg aagcggacgc tggcgccggc gccgcgggcg agctcgtaga
     5461 gcggctggta ggcgggccag aggcagacga cctcgtcgcc cggctcgagc agcaggttgt
     5521 gcaggatcag cagcgcctcg atcgcgccga ccgtgaccat cacgttctcg cgcgcggccg
     5581 gcgggttgag cgccgcgatc gcgtcgcgca gctcggggta gccggacgag ggcgtgtagc
     5641 cgacctccca gtcgggggcc ggcggctcgc cgacgatctc ggtcagctcg gcggcggtca
     5701 tcggctcggc gccgctgagc gccagcatca gctcgcagct gaactcgtag tcctcgaaga
     5761 actcctcctg gcggaagacc gcgagcttca tgcgacggct ccctgcgcgc tggcgctcgc
     5821 ggcgcccggg gcgacggctg cgctgcccgc ggtggccgac gccgcgccgg tcacggc
//
LOCUS       DATABV010000109         8831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1719042, whole genome shotgun sequence.
ACCESSION   DATABV010000109 DATABV010000000
VERSION     DATABV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719042"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VHF88_04600"
     CDS             complement(<1..342)
                     /locus_tag="VHF88_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX2161084.1"
                     /translation="MSESPTSTSQATEAATPGLRDTPTLILGGGPAGLTAGYLLAKQG
                     FPVIIFEAEDQVGGIAKTEVRDGYRFDLGGHRFFTKVQEVDDLWHEIMREEFLKRPRM
                     SRIYWNKKFLDY"
     gene            complement(385..1989)
                     /locus_tag="VHF88_04605"
     CDS             complement(385..1989)
                     /locus_tag="VHF88_04605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2161085.1"
                     /translation="MSTVAAPRTRPLARVSARIPPRVATFVSSPAGIALLVVLLLLIS
                     LYLRTRALGASLWMDEGLSIGIAGQPFFDIPGVLRQDGSPPLYYMLLHGWMELVGNSP
                     ADTQGLSVAIALLAVPGGLWAGWSLFGRRAGLICAALAAVNPFLTAYAQETRMYSLMV
                     VLSLVATAAFLHVFVYRHRRYLPLFAAVMAALLYTHSWGIFATAGAVLTFAFVWFRSD
                     ERGPLLKDAALGFGIAFLLYLPWIPTLLYQAAHTGAPWLNPPRFGVAIQVSKSLLGGG
                     SVTVALVLAAGSGIAAILFRRADDRERRAVYATIAIGVFTLAVAWLFSQISPAWTTRY
                     LGVALGPVLILAALGLARAGTLGLVAFVIVFGIWGVTRSGGLENKSNAADLAAAVQGT
                     LQPGDLVVTLQPEQAPLMHYHLPGGLQGDLVEATQLGEVDTKGVMDWRDVQERLEAAT
                     PEKNLEPLLDRLPRSQRVLLVYPVTTNIGDWDAPWTETVRRRAAQWGRALELDDRFEL
                     DGAVPPFYRRAGRIGVRGVLYTKTAD"
     gene            complement(2004..3518)
                     /locus_tag="VHF88_04610"
     CDS             complement(2004..3518)
                     /locus_tag="VHF88_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161086.1"
                     /translation="MPEERSSPVAPVLIGCLALSALSLFFPSTPTYDPWAWIMWGREI
                     VHLDLVTTGGPSWKPLPVLFTTPFALFGADVAPYLWLWIARAGGLLALAMTFRLARRL
                     VGRGPAGWIAGVFAAGFLATTYEYVRDAALGNSEAMLAGLGLWAVERHLDGRRDHALY
                     LGFAAALLRPEVWPFLGLYGIWLWFKDPELRLRMAILAALIPLLWFGPELWGSGDALR
                     ASTRANNPNPGSAAFAEHPGLEVVERFHHRTVWALEAAGLIGMLTAAVAWRRRRRQGA
                     ILFIAGLGLAWLLLVSAMTEGGYAGNQRYLIVTTAALCVLGGIGVARAFEGIQLLGER
                     IAGNARAGIAAACVAFALGLVVGWPVVRDKADNIDATLDELRYEASLFHTLDDAVAVA
                     GGRDRLLACGAVYSGPFQTQMIAYELGVHGLDVRALEGTPAPGVAFRTHTIPEGPLVI
                     KVTDDRFRQVAADGRWLIWTAPRADATGRACPAAGPDAPRVAPSDRTPELASSR"
     gene            complement(3521..4480)
                     /locus_tag="VHF88_04615"
     CDS             complement(3521..4480)
                     /locus_tag="VHF88_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2161087.1"
                     /translation="MNKRFALFQVVFSVVALGAVIWWASHQDAPEIPSGSDAIAWLAL
                     ALGLYALETVIRAERWHKILAESRIEASRADCYGLSTVGYMGNNVLPARAGEALRVVL
                     LDGKTGAGKRKILGTIVVERLLDLIALVVIFVLTVYLVLEGDVVPTDEPLLIAGIGFA
                     ALALAAVALWMVHRRGGLARIREIVSPLIHAPRALLNRQGLLLLTVTFALWAIEGAVY
                     LSVARAIDLQISTSGALYLVALTNFVSALPAAPGSIGTFDAAVAFGAKALGASGSVAV
                     TYLLLLRFVLYVPITIVGLIVLVSRYGGWARLRQATHLETSSV"
     gene            4645..5082
                     /locus_tag="VHF88_04620"
     CDS             4645..5082
                     /locus_tag="VHF88_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161088.1"
                     /translation="MTTRATLLAALCVLAAASGCGDDDGPPSSQQQPAGDTVAITAPG
                     ETAPAPGPATTLAPEDKRGLALECIRDEKGIEASTEGDKAIAIGPGGDASRIDFYQSS
                     LEAEAVQFEGRAEGAEQIGAALFYVRELPEQTLIDIEDCLNEQ"
     gene            5072..6658
                     /locus_tag="VHF88_04625"
     CDS             5072..6658
                     /locus_tag="VHF88_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2161089.1"
                     /translation="MSSSRDGGPRLSRRRLLQAAGVAGAGAALPLAAAGCGLGDDGAG
                     DGEPENVVLLIVDTLRPDFVGAYGAPRMRTPNFDRLIASGLRFNRAYPEAMVTVPARR
                     SIFTGQRIFPYRDFKPIADLGISPGWTGIDDLDRTLMKTLKDAGYRTIQVTDNPHTGF
                     TQTYKPFRLSWDTFVSIEGRTGTRNPPETVSDAEVRRWLPKELRDDERYVVGMKKNLA
                     NTGYGRDDSESDAARVFGEAADQLSDAARSGEKFALVVDCFDPHEPWSPPKEYIDLYG
                     DPSYEGPEIGVLDYGPAGYMSKDELRRAHADYAAAVTMTDRWLGTFLDRFEELGLADN
                     TAIVLLSDHGVLLGERGWVGKIPGELHPEMAQVPFAIVHPAGKRAGESSDYFASTHDV
                     APTILSMLGIEPPRWMNGADLSLLLDSSRGAGERQPEARPFHYGGMYNRYYIRTDQWV
                     LLGDTLGGNRQLFDLTLDPGEWRDVATASRANRRLTEELYQQVIEATGGEPLPYYDDE
                     KLQAQLAAAREKAGFVDPTP"
     gene            complement(6668..>8831)
                     /locus_tag="VHF88_04630"
     CDS             complement(6668..>8831)
                     /locus_tag="VHF88_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2161090.1"
                     /translation="ERTGDDPPRRQPPSVLLSFLHLAVLSAFALAQPLFDLLSDNPEF
                     FAARGSTATDIIVFGVLLVLVPPALLLAIELLVGLAGSAARRGAHLVLMALLAGIVFV
                     QALKDAVGASDFVLIALALALGALAAVLYARAEPVRSFLTVLSPAPLVFLILFLFISP
                     VSKITLADDASAQTIGGIKPVPVVMVVFDELPGTSLLDAEGAVDPVRYPGFAQLAEDG
                     TWFPNAHTIYDATAKAVPAIMDGNYPEKGVLPTSSEHPNSIFALLGKSHTMNVSEEAT
                     TVCPRDLCDDERLDESFLSRLGSTSEDLGLVYAHVVAPPDIESDLPSVSETWGDFGGE
                     EGAAGVGEVEAEADDGEIDTHREGRKETLANLKSDRGAKFDEWIAAIRGGERPSLNFK
                     HVLLPHVPWKYLPTGQLYNTAAGDPIPGLSRQSYPDETQVQQLQLRHLLQLGYADLEI
                     QKMIDRLKEVGIYDEALVVVLADHGVAFEQGVFDRRRAKPETLESIAPVPLFVKLPGQ
                     RRGRVNDSIVETTDVLPTIADLLDIDLPEPTDGKSVFSDAVARRDEVKVRPRDFSDWM
                     RVDPDEFAARKQALIDRKVALFGSGADGPDRIYRVGPNQQLVGRPVSSLAVSGESSLE
                     VALTDSDAYDDVDVDGPFLPVWVTGEVSGDTGGEPRDVAIAVNGTVRAVSNTFRLANG
                     DQDLIAAMIPPSSLRDGANAVEVYEVGPDLTLAPIRQS"
BASE COUNT         1432 a         3035 c         3230 g         1134 t
ORIGIN      
        1 gtagtcgagg aacttcttgt tccagtagat ccgcgacatg cgcgggcgct tgaggaactc
       61 ctcgcgcatg atctcgtgcc agaggtcgtc gacctcctgg accttggtga agaagcggtg
      121 gccgccgagg tcgaagcggt agccgtcgcg gacctcggtc ttggcgatgc cgccgacctg
      181 gtcctcggcc tcgaagatga tcaccggaaa gccttgcttg gcgagcaggt agccggccgt
      241 caggccggca ggaccgcccc cgaggatcag ggtcggggtg tcgcgcaggc cgggcgtagc
      301 cgcctcggtc gcctggctcg tgctggtcgg gctctcgctc atccggtctc gctcgcttgt
      361 cggagggtcg ctgtcgtctg aggtctagtc agcggtcttg gtgtagagga cgccccgcac
      421 cccgatccgg ccggcccgcc ggtagaaggg cgggaccgcg ccgtcgagct cgaagcggtc
      481 atccagctcg agcgcccgtc cccactgggc cgccctgcga cggaccgtct ccgtccaagg
      541 ggcgtcccaa tcgccgatgt tcgtcgtcac cggatagacg agcagcactc gctgagagcg
      601 cggcagccta tcaagcagcg gctcgaggtt cttctcgggc gtggcagcct cgagacgctc
      661 ctggacgtcg cgccaatcca tcactccctt ggtgtcgacc tcgccgagct gggtcgcctc
      721 gacgaggtcc ccctggaggc cgccggggag gtggtagtgc atcagcggcg cctgctccgg
      781 ctgcagcgtg acgacgaggt cgccgggctg cagggtgccc tggacggcgg cggcgaggtc
      841 ggccgcgttg gacttgttct cgagcccgcc gctgcgggtc actccccaga tcccgaagac
      901 gatcacgaag gcgaccagtc cgagggtgcc ggcgcgggcg agcccgagtg ccgcgaggat
      961 cagcaccggt ccgagcgcga cgccgaggta gcgcgttgtc caggctggcg agatctgcga
     1021 gaacagccag gcgaccgcga gcgtgaagac cccgatcgcg atcgtcgcgt agaccgcgcg
     1081 gcgctcacga tcgtccgcgc ggcggaagag gatcgcggcg atccccgaac ccgcggcgag
     1141 gaccagcgcg acggtgaccg acccgcctcc gagcagcgac ttcgacacct ggatcgcgac
     1201 gccgaagcgg ggcgggttga gccatggggc gccggtgtgg gctgcctggt agagcagggt
     1261 cgggatccac ggcaggtaga gcaggaacgc gatgccgaag ccgagcgcgg cgtctttgag
     1321 cagcggaccg cgctcgtcgc tgcggaacca gacgaacgcg aaggtcagga cggcgcctgc
     1381 ggtcgcgaag atgccccagc tgtgcgtgta gagcagcgcc gccatcacgg cggcgaacag
     1441 cggcaggtag cgtcggtggc gatagacgaa gacgtgcagg aaggcagcgg tcgcgaccag
     1501 gctgaggacg accatcagcg agtacatccg cgtctcctgc gcatacgccg tcaggaatgg
     1561 gttgacggct gccagcgcgg cgcagatcag gcccgcgcgg cggccgaaca gcgaccagcc
     1621 ggcccagagg ccgcccggga ccgcgagcag cgcgatcgcg accgacaggc cctgggtgtc
     1681 ggccgggctg ttgccgacga gctccatcca gccgtgcagc agcatgtagt agagcggcgg
     1741 cgagccgtcc tggcgcagga cgcccgggat gtcgaagaac ggctgcccgg cgatcccgat
     1801 cgacagcccc tcgtccatcc acagcgacgc gccgagggcg cgggtgcgca ggtagagcga
     1861 gatcagcagg agcaggacga cgagcagcgc gatgccggcc gggctcgaca cgaacgtcgc
     1921 gacgcgcggc ggaatccgcg ccgagacgcg cgcgagcggg cgcgtacggg gggctgcgac
     1981 ggtggacatc gcgggaagcc tagtcagcgc gaggacgcaa gctcgggggt acggtcgctg
     2041 ggcgcgacgc gcggcgcgtc cggccctgcc gccgggcagg cgcgaccggt ggcgtccgcc
     2101 cgcggggcgg tccagatcag ccagcgcccg tcggcggcaa cctggcggaa gcggtcatcg
     2161 gtgaccttga tcaccagcgg gccctcgggg atcgtgtggg tgcgaaacgc gacccccgga
     2221 gccggcgtcc cctcgagggc ccgcacgtcg aggccgtgga cgccgagctc gtaggcgatc
     2281 atctgggtct ggaaggggcc gctgtagacg gcgccacagg cgagcagacg gtcacggccg
     2341 ccggcgaccg cgacggcgtc gtcgagcgtg tggaagaggc tcgcctcgta gcgcagctcg
     2401 tcgagcgtcg cgtcgatgtt gtcggccttg tcgcggacga ccggccagcc gacgacgagg
     2461 ccgagcgcga aggcgacaca ggccgcggct atgccggccc gcgcgttgcc ggcgatccgc
     2521 tcgccgagca gctggatgcc ctcgaaggcg cgtgcgacgc cgatcccgcc caaaacgcag
     2581 agcgcagcgg tcgtgacgat cagatagcgc tggttgccgg cgtagccacc ctcggtcatc
     2641 gcgctgacga gcagcagcca agcaagcccg agcccggcga tgaacaggat cgcgccttgg
     2701 cgacgccggc ggcgccatgc gaccgccgcg gtcaacatgc cgatcaggcc ggcggcctcc
     2761 agcgcccaga cggtgcgatg gtggaagcgc tcgacgacct cgagccccgg gtgctcggcg
     2821 aaggcggcgc tgccggggtt ggggttgttc gcgcgcgtcg acgcgcgcag ggcgtcgccg
     2881 gagccccaca gctccggccc gaaccagagc agcgggatca gcgcggcgag gatcgccatc
     2941 cgcaggcgca gctcgggatc cttgaaccag agccagatcc catagagacc gaggaacggc
     3001 cagacctccg gccgcagcag cgcggcggcg aagccgagat agagcgcgtg gtcgcgacgg
     3061 ccgtcgagat gccgctcgac cgcccacagg ccgaggccgg cgagcatcgc ctccgagttg
     3121 ccgagcgccg cgtcgcgcac gtactcgtag gtcgtcgcca ggaaccccgc cgcgaagacg
     3181 cccgcgatcc agcccgccgg tccccgcccg accagccgcc gcgcgagccg gaaggtcatg
     3241 gcgagcgcga gcagcccgcc cgcgcgggcg atccagagcc agaggtaggg cgcgacgtcg
     3301 gcgccgaaga gcgcgaacgg cgtcgtaaag agcaccggca gcggcttcca cgacggcccg
     3361 ccggtcgtca cgagatccag gtggacgatc tcgcggcccc acatgatcca cgcccacggg
     3421 tcgtaggtcg gcgtcgaggg aaagaagagg ctgagcgcgg atagggccag gcagccgatc
     3481 agcaccggcg ccaccggcga cgagcgttcc tcgggcacgg tcagacgctg ctggtctcga
     3541 ggtgggtcgc ctggcgcagg cgcgcccagc cgccgtagcg actgaccagg acgatcaagc
     3601 cgacgatcgt gatcgggacg tagaggacga agcgcagcag caggaggtac gtgacggcga
     3661 ccgagccgct ggcgccgagc gccttggcgc caaaggcgac agccgcgtcg aaggtgccga
     3721 tcgagcccgg cgccgcgggc agcgcggaga cgaagttggt cagcgcgacc aggtagaggg
     3781 caccggaggt gctgatctga agatcgatcg cccgcgcgac cgagaggtag acggcgccct
     3841 cgatcgccca cagcgcgaag gtgacggtca gcagtagcag tccctggcgg ttcagcagcg
     3901 cacgcggcgc gtggatcagc gggctgacta tctcgcgtat gcgcgccagg cctccgcggc
     3961 gatggaccat ccagagcgcg accgcggcga gcgccagtgc tgcgaagccg atcccggcga
     4021 tcagcagcgg ctcgtcggtc ggtacgacgt ccccctcgag caccaggtag acggtcagga
     4081 cgaagatgac gacgagcgcg atcaggtcga gcaggcgctc gacgacgatc gtgccgagga
     4141 tcttgcgctt gccggcgccc gtcttgccgt cgagcaggac gacgcgcagc gcctcgccgg
     4201 cgcgcgccgg caggacgttg ttgcccatgt agccgaccgt cgagagcccg tagcagtccg
     4261 cacgcgaggc ctcgatccgg ctctccgcga ggatcttgtg ccagcgctcg gcgcggatga
     4321 ccgtctccag cgcgtagagc ccgagcgcga gcgccagcca ggcgatcgcg tcgctgcccg
     4381 acgggatctc cggagcgtcc tggtgggacg cccaccagat caccgcgccg agggcgacga
     4441 cggagaagac cacctgaaat agcgcgaagc gtttgttcat cggtcgagca agggtcgcct
     4501 cgcgccggcc gcgccggctt cgcgaggatg agcttcgcgc agcggaacgg cactgcactg
     4561 gacgggtcga cgaggccgcg gcggctcgtc gagaggcgtc aaggtagcag gcgccgccgc
     4621 cggattcact actctgcgcc gtcgatgacg acccgcgcga ccctcctcgc ggctctctgc
     4681 gtgctcgccg ccgcttccgg ctgcggcgac gacgacggcc ctccctcgag ccagcagcag
     4741 ccggccggcg acacggtcgc gatcaccgcc cccggcgaga cggcaccggc gccaggacca
     4801 gcgacgacgc tcgcgcctga ggacaagcgc ggcctcgcgc tcgagtgcat acgcgacgag
     4861 aagggcatcg aggcgagcac cgagggcgac aaggcgatcg cgatcggccc gggcggtgac
     4921 gcttcgcgga tcgacttcta ccagtcgtcg ctcgaggccg aggcggtcca gttcgagggc
     4981 cgagccgagg gcgccgagca gatcggcgcc gcgctcttct acgttcgcga gctgcccgag
     5041 cagacgctga tcgacatcga ggactgcctc aatgagcagt agccgggacg gcgggccgcg
     5101 cctcagccgc cgtcgcctgc tccaggccgc tggtgtcgcc ggcgctggag ccgccctgcc
     5161 gctcgccgcg gcgggttgcg gcctcggcga cgacggcgcg ggcgacggtg agcccgagaa
     5221 cgtcgtcctg ctgatcgtcg acacgctgcg gcccgacttc gtcggcgcct acggcgcgcc
     5281 gcggatgcgg acacccaact tcgacaggct gatcgccagc ggcctgcgct tcaaccgcgc
     5341 ataccccgag gcgatggtga ccgtcccggc gcgacggtcg atcttcaccg gccagcggat
     5401 cttcccctat cgcgacttca agccgatcgc cgacctcggc atcagccccg gctggaccgg
     5461 gatcgacgac ctcgaccgga cgctgatgaa gacactcaag gacgcgggct accggacgat
     5521 ccaggtgacc gacaacccgc acaccggctt cacgcagacc tacaagccgt tccggctgag
     5581 ctgggacacc ttcgtctcga tcgagggtcg caccggcacg cgcaacccgc cggagaccgt
     5641 ctccgacgcg gaggttcgtc gctggctgcc gaaggagctg cgcgacgacg agcgctacgt
     5701 cgtggggatg aagaagaacc tcgccaacac gggctacggg cgcgacgaca gcgagagcga
     5761 cgccgcgcgc gtcttcggcg aggccgcgga ccagctctcg gacgccgccc gcagcggcga
     5821 gaagttcgcg ctcgtcgtcg actgcttcga cccgcacgag ccgtggagcc cgccgaagga
     5881 gtacatcgac ctctacggcg accccagcta cgagggcccg gagatcggcg tgctcgacta
     5941 cggcccggcg ggctacatgt ccaaggacga gctgcggcgc gcacacgccg actacgcggc
     6001 cgcggtcacg atgaccgacc gctggctcgg aaccttcctc gaccgcttcg aggagctcgg
     6061 cctggccgac aacacggcga tcgtgctgct gtcggaccac ggcgtgctgc tcggtgagcg
     6121 cggctgggtc ggcaagatcc ccggcgagct gcatcccgag atggcccagg tgccgttcgc
     6181 gatcgtccac ccggccggca agcgcgccgg cgagagcagc gactacttcg cctcgaccca
     6241 cgacgtcgct ccgacgatcc tgtcgatgct ggggatcgag ccgccgcgct ggatgaacgg
     6301 cgccgacctg tcgctgctgc tcgactcctc ccggggcgcg ggcgagcggc agcctgaggc
     6361 gcggccgttc cactacggcg gcatgtacaa ccgctactac atccgcaccg accagtgggt
     6421 gctgctcggc gacaccctcg gcgggaaccg ccagctcttc gacctgacgc tcgaccccgg
     6481 cgagtggcgc gacgtggcga ccgccagccg cgccaaccgg cggctcaccg aggagctcta
     6541 ccagcaggtg atcgaggcga cgggcggcga gccgctgccc tactacgacg acgagaagct
     6601 gcaggcgcag ctcgcggccg cgcgcgagaa ggccggcttc gtcgatccca ccccgtaggg
     6661 cgccgcgtca ggactgccgg atcggcgcca gggtaaggtc ggggccgact tcgtatacct
     6721 cgacggcgtt ggcgccgtcg cgcagggagc tgggcgggat catggcggcg atcaggtcct
     6781 ggtcgccgtt ggcgagccgg aaggtgttcg agacggcgcg cacggtgccg ttgaccgcga
     6841 tcgcgacgtc gcgcggctcg ccgcccgtgt cgccggagac ctcgccggtc acccagaccg
     6901 gcaggaacgg gccgtcgacg tcgacgtcgt cgtaggcgtc ggagtcggtc agcgcgacct
     6961 ccagcgagct ctcgccgctc acggcgagcg aggagacagg gcgcccgacg agctgctggt
     7021 tgggcccgac gcgatagatc cggtcggggc cgtcggcgcc ggatccgaac agcgcgacct
     7081 tgcggtcgat cagcgcctgc ttgcgcgcgg cgaactcgtc cgggtcgacg cgcatccagt
     7141 cggagaagtc gcgcggccga accttgacct cgtcgcgccg agcgacggcg tcgctgaaga
     7201 ccgacttgcc gtcggtcggc tcgggcaggt cgatgtcgag caggtcggcg atcgtcggca
     7261 ggacgtcggt cgtctccacg atcgagtcgt tcacgcgccc gcgcctctgc cccggcagct
     7321 tcacgaacag cggcaccgga gcgatcgact cgagcgtctc gggcttggcg cggcggcggt
     7381 cgaagacgcc ctgctcgaag gcgacgccgt ggtcggcgag cacgaccacg agcgcctcgt
     7441 cgtagatgcc gacctccttc aggcggtcga tcatcttctg gatctcgagg tcggcgtagc
     7501 cgagctgcag cagatgacgc agctgaagct gctggacctg cgtctcgtcg ggatagctct
     7561 ggcgcgacag gccggggatc gggtcgccgg cggccgtgtt gtagagctgg ccggtcggca
     7621 ggtacttcca cggcacgtgc ggcagcagga cgtgcttgaa gttcagcgac gggcgctcgc
     7681 cgccgcgaat cgcggcgatc cactcgtcga acttcgcgcc gcgatcggac ttcaggttgg
     7741 ccagcgtctc cttcctgcct tcccggtgcg tgtcgatctc gccgtcgtcg gcctcggcct
     7801 cgacctcgcc gacgccggcc gcgccctcct cgccgccgaa gtcaccccag gtctccgaga
     7861 ccgagggcag gtcggactcg atgtcgggcg gcgcgacgac gtgggcgtag acgagaccga
     7921 ggtcctcgct agttgatccg aggcggctga ggaacgactc gtcgaggcgc tcgtcgtcgc
     7981 agaggtcgcg cgggcagacc gtggtggcct cctccgagac gttcatcgtg tggctcttgc
     8041 cgagcagcgc gaagatgctg ttcgggtgct cggaggacgt cggcaggacg cccttctcgg
     8101 gatagttgcc gtccatgatc gccgggaccg ccttggcggt cgcgtcgtag atcgtgtggg
     8161 cgttggggaa ccaggttccg tcctcggcca gctgggcgaa gccggggtaa cggacggggt
     8221 cgaccgctcc ctcggcatca agcagcgacg tccccggcag ctcgtcgaag acgaccatca
     8281 cgaccgggac cggcttgatg ccgccgatcg tctgcgccga ggcgtcgtcg gcaagcgtga
     8341 tcttcgacac cggcgagatg aacaggaaga ggatcaggaa gaccagcggc gccgggctga
     8401 gcacggtcag gaacgagcgc accggctcgg cacgcgcgta gaggacggcg gccagcgcgc
     8461 cgagtgccag cgcgagcgcg atcagcacga agtcgctcgc cccgacggcg tccttgagcg
     8521 cctgcacgaa gacgatgccg gcgagcaggg ccatcagcac gagatgcgca ccgcggcgcg
     8581 ccgccgagcc ggcgaggccg accagcagct cgatcgccag cagcagcgct ggcgggacca
     8641 gcaccaacag cacgccgaag acgatgatgt cggtcgccgt cgagccgcgc gcggcgaaga
     8701 actccgggtt gtcggagagc aggtcgaaca gcggctgggc gagcgcgaag gcggaaagga
     8761 ccgcgagatg caggaacgac agcaggaccg acggcggctg ccgtcgaggg ggatcgtccc
     8821 ccgtccgctc g
//
LOCUS       DATABV010000110         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1156963, whole genome shotgun sequence.
ACCESSION   DATABV010000110 DATABV010000000
VERSION     DATABV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156963"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1530
                     /gene="dnaA"
                     /locus_tag="VHF88_04635"
     CDS             199..1530
                     /gene="dnaA"
                     /locus_tag="VHF88_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX2161091.1"
                     /translation="MRDQLEPIWDRVRTEMRRDVADFKFHIWLDPLELVGVDRQTLFV
                     RAPDHIRSWVRDRYLPLVREAAERGYAPGAVVEIVDEQWTASDGVGEPPQTAAPDTDS
                     ALNPKYTFEQFVIGKGSRFAHAAALSVAEMPGQAYNPLFIHGRPGLGKTHLLHAIGNY
                     VQRYGSGLSVRYATVEQFTNEFVDALRARDTQAFKERFRGVDVLLLDDVQFLADKART
                     EEELFHTFNELHDSGRQLVMTSDRSPAELQGVAERLSERFGSGLVVSVDPPDFHVRRA
                     ILEKRARLDAVDASPDLVSEIAGRVTTSVRALEAALIQVVAYASLRGEQPTPDIARKL
                     LDKLGPDRRTAKPSVDDVVTATASLFGLTANQLVARDRRPDVARARKVAIYLARELTG
                     KSLPEIGRRLGGRDHSTILAAVRSLSGDIERDSELAQAVDKLKRQLAAPAS"
     gene            1661..2770
                     /gene="dnaN"
                     /locus_tag="VHF88_04640"
     CDS             1661..2770
                     /gene="dnaN"
                     /locus_tag="VHF88_04640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX2161092.1"
                     /translation="MKLVLSRDSFLERLGVVARGVSTRSAIQTLSGVLLSVESGSAEL
                     QATDTELGIRANVDAQVERDGAVVVPGRLLLDVARSLPADSVTIEYRASEQDVEVKSG
                     PSTFHLRTLPLEDFPKLPVASADATVSVPAAAFVETVDRVARSASRDETRPHLTGVLV
                     TATGNTLRMVATDSYRLSVKETPLEHPLSGDLEVNVPARTLQELSRIAAASGAEQISV
                     SALENQVVFSVGDVVLSSRLVEGRFPNYQQLLPEAYEHELRVDRGELLEVVRRVSLLA
                     QKNAPLRLSFGEGQLEVSAQTPDIGEASESLPVPFNGEPLAIGFNPEFFRDGLDSAET
                     DEIVLKLISPLRPGLIQSGADGGFIYLVMPIRLNV"
     gene            2781..3869
                     /locus_tag="VHF88_04645"
     CDS             2781..3869
                     /locus_tag="VHF88_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX2161093.1"
                     /translation="MRVTELSARNFRNYERADVELGERLSVIVGPNGAGKTNLLEAVY
                     FGCTGRSPRTSNDRDLLRRGAPAARVTVTTADAHGGEHQLEAAFQPGEPKLLRVDGTA
                     VDNLWATDLRPLISVFLPERLELVKGAPANRRAHLDQLVAALWPARASARAAYSRALA
                     QRNALLGRIRAGNAAPDSLGAWDAELARHGAELMTARGDAVGLLAPRFTARAAELGLS
                     ERAEIAYRPRSQAEAADGLAAELFERRAADLERGFTAHGPHRDDIRLSHASEPLRGFG
                     SQGQQRVALLALLFAERDLLAAERDRPPLMLLDDVMSELDAGRRGLLADLLREGGQGV
                     VTATELEHVPGHDEPGVTVLRVAGGEVS"
     gene            3866..4174
                     /locus_tag="VHF88_04650"
     CDS             3866..4174
                     /locus_tag="VHF88_04650"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX2161094.1"
                     /translation="MRRRSPRPLATALEQLGHAAQPPTLLARVQAAWAEEVGPAIAAE
                     AQPVRERDGTVTIACRSAAWASELELLAPELRERLNGALGDPADGPLKELRAKVGKLP
                     "
     gene            4331..>4969
                     /locus_tag="VHF88_04655"
     CDS             4331..>4969
                     /locus_tag="VHF88_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2161095.1"
                     /translation="MADNGKAQSYGAEDISVLEGLEAVRKRPGMYIGSTGIRGLHHLI
                     YEVMDNSVDEALAGEADQVDIVIHPDNRVTVTDNGRGIPVGIMEKEQRPAAEVVLTVL
                     HAGGKFGDGGGYKVSGGLHGVGVSVVNALSERLDLTIWTDGFEHTQSYERGTPTGPLT
                     KGAKTDRRGTSITFLPDLEIFETIDYEYATLETRFREMAFLTRGLKITFTDER"
BASE COUNT          770 a         1778 c         1665 g          756 t
ORIGIN      
        1 gcgagcgtga gccgccgggc ggaggcctcg tgctccatgg cggaggcggt gccctcgcga
       61 tttgcggcag tcgtccggag agcccggtaa acgcgtgtcg cgcggatgta gactcgccaa
      121 ccccgaggac gaggccccgc gtcgagccct ctccaaccaa tgtctgccga acaggtcccg
      181 gagggccgag tacttcctgt gcgcgatcag ctggagccga tatgggatcg cgtccgcacg
      241 gagatgcggc gtgacgtcgc ggacttcaag ttccacatct ggctcgaccc gctcgagctc
      301 gtcggcgtcg accgtcagac gctcttcgtc cgagcccccg accacatccg atcgtgggtg
      361 cgcgaccgct acctgccgct cgttcgcgag gccgccgagc gcggctatgc cccgggcgcc
      421 gtggtcgaga tagtcgacga gcagtggacg gcgagtgacg gcgtcgggga gccgccgcag
      481 acggcagcgc cagacaccga cagcgcgctc aatcccaagt acaccttcga gcagttcgtg
      541 atcgggaagg gaagccgctt cgcgcacgca gcggcgctct cggtcgcgga gatgcccggc
      601 caggcctaca acccgctgtt catccacggc cggcccggac tgggcaagac tcacctccta
      661 cacgcgatcg gcaactacgt gcagcgctat ggatccggcc tctcggtccg ctacgccacc
      721 gtcgagcagt tcaccaacga gttcgtcgac gccttacgcg ctcgtgacac gcaggcgttc
      781 aaggagcgct ttcgcggagt cgacgtgctc ctactcgatg acgtccagtt cctcgccgac
      841 aaggcgcgca cggaggagga gctcttccac acgttcaacg agctccacga ctcgggccgc
      901 cagctcgtca tgacgtcgga ccgcagcccg gccgagctcc aaggagtcgc cgagcgcctc
      961 agcgagcgct tcggatctgg gctggtcgtc tcggtagacc cgccggactt ccatgtccgc
     1021 cgggcgatcc tcgagaagcg ggcgcgactc gacgccgtcg acgcctcgcc tgacctcgtc
     1081 tccgagatcg ccggtcgcgt cacgacgagc gtgcgtgcgc tcgaggcagc gttgatccag
     1141 gtcgtcgcct acgcctcgct gcgtggagag cagcccaccc ccgacatcgc ccgcaagctg
     1201 ctcgacaaac tcggtccgga ccgacgcacg gccaagccga gcgtggacga tgtcgtcaca
     1261 gcgaccgcca gcctcttcgg cttgacggcc aaccagctcg tcgcgcgcga tcggcgcccc
     1321 gacgtcgccc gggcacgcaa ggttgcgatc tacctcgccc gcgagctgac gggcaagagc
     1381 ctgcccgaga tcggacgccg gctcggaggc cgcgaccaca gcacgattct cgccgccgtt
     1441 cggagcctct ccggcgacat cgagcgcgac agcgagctcg cgcaagctgt ggacaagctc
     1501 aagcgtcagc tggcagcgcc agcgtcatga ccggcggtgc tgcaagactc cacacttctc
     1561 cacagcttgc gcgtggcctt gcttcagctc taccgcgggc tatatccgct gcaatcaaca
     1621 tctccacagc acctatccct tctagaaata ggtagaccag ttgaagctcg ttctttcacg
     1681 tgattcattt ctcgagcgac tcggcgtcgt tgcccgcggc gtctcgactc gcagcgcgat
     1741 ccagacgctc tccggcgtcc tgctcagcgt ggagtcgggc tcggcggagc tgcaggcgac
     1801 cgacaccgag ctgggcatcc gcgccaacgt cgatgcccaa gtcgagcgtg atggcgccgt
     1861 agtcgttccc ggccgcctgc tgctcgacgt cgcgcggtcc ttgccggcgg actcggtcac
     1921 gatcgagtac cgcgcctccg agcaggacgt cgaggtcaag tccgggccct ccacgttcca
     1981 tctccgcaca cttccgctcg aggacttccc gaagctgccg gtcgcgagcg ccgacgccac
     2041 ggtctcagtc ccggcggcag ccttcgtcga gaccgtcgat cgcgtcgccc gctcggcttc
     2101 ccgcgacgag acccggccgc acctcaccgg cgtcctcgtg acggccaccg gcaacacgct
     2161 gcgcatggtc gccaccgact cctaccggct ttcggtcaag gagacaccgc tggagcatcc
     2221 gctcagcggc gacctggagg tgaacgttcc ggcccgaacc ttgcaggagc taagccgcat
     2281 cgcagctgcc tccggcgccg agcagatctc ggtctcggcg ctcgagaacc aggtcgtttt
     2341 cagtgtcggc gacgttgtcc tctcgtcgcg gctcgtcgag ggtcgattcc cgaactatca
     2401 gcagctgctc cccgaggcgt atgagcacga gctgcgcgtg gaccgcggcg agctgctcga
     2461 ggtggtccgc cgcgtgagcc tgctcgcaca gaagaacgct ccgctgcggc tgtccttcgg
     2521 ggaggggcag ctggaggtct ctgcccagac gccggacatc ggcgaggcaa gcgagtcgct
     2581 gccggtgccg ttcaacggcg agccactggc gatcggcttc aacccggagt tcttccgtga
     2641 cgggctcgac agcgccgaga ccgacgagat cgtcctcaag ctgatcagcc cgttgcgccc
     2701 gggcctgatc caatccggcg ccgacggggg cttcatctat ctcgtcatgc cgatccgcct
     2761 caacgtctag ccgcctcgcc gtgcgcgtca cggagctctc ggcgcgcaac ttccgcaact
     2821 acgagcgggc ggacgtcgaa ctcggcgagc ggttgtcggt gatcgtgggc ccgaacggcg
     2881 ccggcaagac gaacctgctc gaggctgtct acttcggctg caccgggcgg tcgccgcgga
     2941 cgtcgaacga ccgcgatctg ctgcgccgag gcgcccccgc ggcgcgggtc acggtgacga
     3001 ccgccgacgc ccacggcggc gagcatcagc tcgaggccgc cttccaaccc ggcgagccga
     3061 agctgctgcg cgtcgacggg acggctgtgg acaacctgtg ggcgaccgat ctgcgtccgc
     3121 tgatcagcgt cttcctgcct gagcgactgg agctcgtcaa gggagccccg gcgaaccgtc
     3181 gtgcgcacct agaccaactc gtcgccgcac tgtggccggc gcgggccagc gcccgtgccg
     3241 cctactcgcg cgcgctcgcg cagcgcaacg cgctgctcgg acgcattcga gccggtaacg
     3301 cagcgccgga ctcgctcggc gcctgggacg ccgagctcgc gcgccatgga gccgagctga
     3361 tgacggctcg cggtgacgcc gtcggcctgc tcgctccccg tttcacggct cgtgccgccg
     3421 agctcggcct gtccgagcgc gctgagatcg cctaccggcc acgctcgcag gcggaggcgg
     3481 cagacggcct cgcagccgag ctgttcgagc gccgggcggc cgacctcgag cggggcttca
     3541 ccgcgcacgg cccgcaccgc gacgacatcc ggttgtcgca cgcgtccgag ccgctgcgcg
     3601 gcttcggctc tcagggccag cagcgggtcg ccttgctcgc gctgctcttc gccgagcgcg
     3661 acctactagc agctgagcgc gatcgcccgc cgctgatgct gctcgacgac gtgatgtcgg
     3721 agctcgacgc ggggcgccgc ggtctgctcg ccgacctgct gcgcgagggc ggtcagggcg
     3781 tcgtgaccgc gaccgagctg gagcacgttc ccggccacga cgagccggga gtcacggtcc
     3841 tccgggtcgc aggcggtgag gtctcatgag gcgccgctcg ccgcgaccgc tcgcgaccgc
     3901 cctcgagcag ctcggccacg ctgcgcagcc gccgacgctg ctcgcgcgcg ttcaggcggc
     3961 gtgggcggag gaggtcggac cggcgatcgc cgccgaggcc cagcccgttc gcgagcgcga
     4021 cggaacagtg acgatcgcct gccgctcggc ggcctgggcg agcgagttgg agctgctcgc
     4081 gccggagctt cgcgagaggc tcaacggggc tcttggcgac cccgcggacg gtccgctgaa
     4141 ggagctgcgc gcgaaggtcg ggaaactgcc ctgattttgc cgcgatttgc agggattttc
     4201 ggcggcgccg agccgtccgg cggcggcact actctgctag tattcgggtc cgtagttacc
     4261 gcaccccgac gccgcgttcc gaatgcgccg cgccggggtc ttctcatgtt tgactgacgg
     4321 aaggcatcgc ttggcggaca acggcaaggc acagagctac ggcgcggagg acatcagcgt
     4381 cctcgagggc ctggaggcgg tccgcaagcg accgggcatg tacatcggct cgacgggcat
     4441 ccgcggcctg caccacctga tctacgaggt gatggacaac tcggtcgacg aggcgctcgc
     4501 cggcgaggcg gatcaggtcg acatcgtgat ccatccggac aaccgcgtca cggtgactga
     4561 caacgggcga ggcatcccgg tcgggatcat ggagaaggag cagcggccgg ccgccgaggt
     4621 cgtgctgacg gttctccacg ccggcggcaa gttcggcgac ggcggcggct acaaggtgtc
     4681 gggcggcctg cacggcgtcg gcgtctcggt cgtcaacgcg ctctccgagc gcctcgacct
     4741 gacgatctgg accgacggct tcgagcacac gcagagctac gagcgcggca cgccgacggg
     4801 cccgctgacc aagggcgcga agaccgaccg ccgcggcacg agcatcacgt tcctgcccga
     4861 cctcgagatc ttcgagacga tcgactacga gtacgcgacg ctcgagacgc gcttccgcga
     4921 gatggccttc ctgaccaggg gcctgaagat caccttcacc gacgagcgc
//
LOCUS       DATABV010000111        13201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2201785, whole genome shotgun sequence.
ACCESSION   DATABV010000111 DATABV010000000
VERSION     DATABV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13201
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201785"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1028)
                     /locus_tag="VHF88_04660"
     CDS             complement(63..1028)
                     /locus_tag="VHF88_04660"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX2161096.1"
                     /translation="MKRHLLSIDGLSREDIERILRRAESFAEVSGREIKKVPTLRGRT
                     VVNLFYESSTRTSSSFELAAKRLSADLVSIKAQGSSVDKGESLKDTVQTLSAYDPAAI
                     VIRSPHVGAAGLVARWTRASVINAGDGKHEHPTQALLDLYTLRRRTGSLDGLRVWIVG
                     DVLHSRVARSGVLAFARMGCRVTVCGPPTLIPHGIEALGCEVRHTLDELGEADVVYAL
                     RMQQERMTGELVPSLREYAARYQINGRRLGPGQLLMHPGPVNRGVELSAEVIDSPQAL
                     IAEQVESGVVVRMAVLYELLTAGQGGGPAAGQHLPTDEPTERQPA"
     gene            complement(1025..1597)
                     /gene="pyrR"
                     /locus_tag="VHF88_04665"
     CDS             complement(1025..1597)
                     /gene="pyrR"
                     /locus_tag="VHF88_04665"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX2161097.1"
                     /translation="MSHKVVLDRDDVRRTLVRIAHEIVERNGSDPLAIVGIHRRGALL
                     AARLRDQVAELVGEPVPLGDVDISFYRDDVGLRGADDPTGPVVHASHVDFPVEETTVV
                     LVDDVLFTGRTVRAAIDALFDYGRPRRVQLAVLADRGHRELPIRPDYVGKNLPTARSE
                     RVNVHVEELDGEDEVTIVSSVDITIRRTDS"
     gene            complement(1682..2617)
                     /gene="rnz"
                     /locus_tag="VHF88_04670"
     CDS             complement(1682..2617)
                     /gene="rnz"
                     /locus_tag="VHF88_04670"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX2161098.1"
                     /translation="MDLDVVFIGTAGSAPTARRGLPATLIRRGGERLLIDCGEGTQRQ
                     LIRSVGLVDLDDVFITHFHADHVLGLPGMLKTFGLQGRERPLNVYGPPGLRQLFRVLA
                     PLIGKPGFDVRTIELESGEDLERDGYVIAAFSVAHRVPAHGYALIEDDRPGRFDEARA
                     RELGVTPGPDFGRLQRGETVQGASGEVEPGQVLGETRLGRRIVFSGDSAPCDMTRGEA
                     HRADLLVHEATFMHEDLDRARETGHSTARQAAMLAADADVALLALTHVSTRYPVGPLR
                     DEARAEFEPTIVPRDFDRIEIPFPERGEPVHHRAE"
     gene            complement(2655..3143)
                     /locus_tag="VHF88_04675"
     CDS             complement(2655..3143)
                     /locus_tag="VHF88_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2161099.1"
                     /translation="MLIAIISDTHLPRGSRRLPERCVELLRGADTIVHAGDLSTVAVL
                     AELEALGPPVVGVCGNVDSPELQRTLSAEATVDAGGVEIGIVHDAGPARGRLARLRRR
                     FPRAAAVVFGHSHIPLHEESDGFQIFNPGSPTERRRSPAHTMGLARVDDGRVEFDLVT
                     LD"
     gene            complement(3194..3595)
                     /locus_tag="VHF88_04680"
     CDS             complement(3194..3595)
                     /locus_tag="VHF88_04680"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEX2161100.1"
                     /translation="MTGSQTAEHPSGGVTASAISNGVVRLLREYTGRGPTRARTHLSD
                     DLVTVVLEDTLTKGERSLVADGETTLVLQARHAYQRTMATDLIELVEQATGRKVRAFL
                     SDNHLDPDIAVESFVLEPSATASASQDGYSD"
     gene            complement(3777..4922)
                     /locus_tag="VHF88_04685"
     CDS             complement(3777..4922)
                     /locus_tag="VHF88_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2161101.1"
                     /translation="MSNAPRVLVVGAGLTGCTLAHRFAREGVETVLLERDSVPGGLVR
                     SDHMNGVLYEPHGSHIFHTEDEEVWNLANEMTPFRHYRHRVDIMVEGKIYNWPILLSD
                     LEDQTRSEEIFAELEQRKGLDAEQRAQAANFEDWCLDLMGPILYERYIRPYTEKQWGR
                     PARELSAQWAPRRVSVRWDNDPYLFPDPFQGWPAGPNGYTDLIDGLLDHDEVTVHTEV
                     DVTGGNIAEQLREHAADVLVLTCPLDVFCGGRFGDLEWRGIAVRNVHIPHVEYAQGAM
                     VVNYPSLDYPFVRIHETKHASGQQCEGTVLGFEFTNAPTRYYPIETERNRSLNNRYQD
                     HLRSELPDGRTFFAGRLANYLYIDMDDCMRQAIDASDEVLDALRAVA"
     gene            complement(4919..6079)
                     /locus_tag="VHF88_04690"
     CDS             complement(4919..6079)
                     /locus_tag="VHF88_04690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2161102.1"
                     /translation="MSADAELVVFSHLRWPWVWQRPHHIVSRLAQGRRTLFVEEPIGT
                     TEVDEPTLRSEPHGSVERVWLEVPDAENAWPGFDHPDATHYGPLLRDQLGDADRVVWL
                     YTPLALPMAQALDPDVLIYDVMDDLASFKDASPLLRRLQDQTLTEAQLVLAGGRSLHD
                     GIKDRADGKSFLFASGVAPEHFQAARSRRRERQRPVAGYVGVIDERVDLALVGELAER
                     LPDWDLVMVGPVTKIDPAAVPQAPNLSYPGLQPYEALPEILGGFDIALMPFQLNEATR
                     SISPTKTLEYLAAGLPVVSTRVPDVVAGYRGVVAFADDAEGFAAACRRTREDGQAERD
                     AKVQPLLHIHHWDTIAARMDHLMVQALAGEPFDDQIPLRADHGGAARKEPAA"
     gene            complement(6076..7437)
                     /locus_tag="VHF88_04695"
     CDS             complement(6076..7437)
                     /locus_tag="VHF88_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX2161103.1"
                     /translation="MSLPFIGAFESTYLPAHDVDVAESSEHVLRWRADLELMRAGGVT
                     RLRYPVRWHRVQPERSVYDWAETDRVLGSMRDNGMRPIVDLVHHTSYPRWLGGFGDPG
                     FGPAYVAYCEAFARRYPWIEEYTLFNEPFATLFLCGHEAIWPPYGRGMGPLVELFRNV
                     LPAIAQASRIVRELLPEARHVYVDTCEGHSALEPGGEGYAAMANDRRFFALDYMLGKL
                     GDGSRPFVRDVVEAGGADLLDAEPGHVDVLGLDYYAHSEWAFRRQRRSEPAGRERHPE
                     PSGIEGVTPSPQPVGPAALAIEYARRYDLPMIFSETNIRGAPADRATWLKYTLDECER
                     AVAAGVPLEGYCWFPFIDSLDWNSLLARADRCIDPVGVYWLDEALERNASSMSRSYAM
                     AAAGAASSELPAYRFTDDVAEWVAGLMPQMEHYDWIDAPDEESGFLDGGIVVARAREE
                     TAA"
     gene            complement(7582..8421)
                     /locus_tag="VHF88_04700"
     CDS             complement(7582..8421)
                     /locus_tag="VHF88_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2161104.1"
                     /translation="MRTLEHIGRVGMGCMPLSLGRGRERGLARETIHAALDAEVVLFD
                     TADAYSTGEGDFGHNERLLAQALAGRREHAFVVATKGGHTRPGGRWELDGSPDYLKAA
                     CEASLRRLGVESIDLYQLHRPDPKVPFAESVGALRDLRDAGKIVHAGLSNVTVDQLVE
                     AEAIVEIAAVQNELSLDYTAPLHNGEVEACEQRGIPFLAWSPLGGIDSADGASRIGAV
                     RAAADRHGVSVQQVVLAWLLSHGEQVVPIPGASRPESIRDSAAARELRLEPDELARIS
                     AAV"
     gene            8585..8657
                     /locus_tag="VHF88_04705"
     tRNA            8585..8657
                     /locus_tag="VHF88_04705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8618..8620,aa:Arg,seq:ccg)
     gene            complement(8667..9398)
                     /locus_tag="VHF88_04710"
     CDS             complement(8667..9398)
                     /locus_tag="VHF88_04710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2161105.1"
                     /translation="MADLSSEGPRRPTPSWFVDELAHAGRENLDADHAVRYDAKEDAA
                     AASEVALLAEHGLTPESLLVELGAGTGQFTVAVAPACGRVVAVDVSPVMLAQLESKLA
                     ALELGNVEIARAGFLTYEHVGAPADFVYSRFALHHLPDFWKALALERVRRTMRVGGIF
                     RLWDVVYDFDPAQAEEGIEAWCATGGDSVEAEWTRAELEEHVRDEHSTYTWLLEPMIR
                     RSGFEILDAEHSQDGIFAKYVLRAA"
     gene            complement(9408..9770)
                     /locus_tag="VHF88_04715"
     CDS             complement(9408..9770)
                     /locus_tag="VHF88_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2161106.1"
                     /translation="MQSSSLVELESSVQGTAHVVAVRGEIDLSSIDAVRRAVDVALSQ
                     RPETVVVDLSRIAFCDSSGIELVVKTHRRAIGQGTRLVVVRPNGPAWRPFELCQIDRE
                     VHFVTSSDEVSSGAATPL"
     gene            complement(9985..10395)
                     /locus_tag="VHF88_04720"
     CDS             complement(9985..10395)
                     /locus_tag="VHF88_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2161107.1"
                     /translation="MASIPLDELELEDAWIDGDESAAWRSASAHTPEHGARSTGSSLL
                     EVGRGCRLPRHTDSAEEVIVVLAGQAEVTVGDSPMAVAANGVALVPKDVPHEVRNAGD
                     EPLRFLALYAGTDVVTRYESPVQPGGDRERKPLG"
     gene            complement(10440..11783)
                     /locus_tag="VHF88_04725"
     CDS             complement(10440..11783)
                     /locus_tag="VHF88_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEX2161108.1"
                     /translation="MGVIAVGRTALVVAGLAAAGCGAAKAGETDVGDRAAVTPASTLD
                     VVGRSLQGSPLRARRVGSARADRVVLVVGEIHGSEPAGRAVVRRLRAARPPRGTALLL
                     VDSANPDGGRAGTRWNARGVDLNRNFPFRWEPIGAPFDTHHSGAGPASEPETAALMRL
                     IRRVRPRVTIWYHQHMRLVDRSGGDPYLERLYAERSRLPHREIGPLPGTATSWQNHAF
                     PRDSAFVVELPAGALGRREADRHADAVIAVARAVAPPRVVETPIPFGPRRRREMRAYA
                     QRHYGIDDFRLRRPRVIVEHYTASNSFASAFNTFAANVPDVELGELPGVCAHYLIDRD
                     GAIHQLVPTSIMCRHTVGLNWTAIGIEHVGVSDAQVMADRRQLNASLRLTRMLQGRHR
                     IATRNVIGHAESLSSPFHRERVARLRRQTHGDFAPAAMKRYRRLLTRMPAPDSLR"
     gene            12073..12807
                     /locus_tag="VHF88_04730"
     CDS             12073..12807
                     /locus_tag="VHF88_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2161109.1"
                     /translation="MSLLRLESVTKSYWRGPHEQRVLRGAALELRAGSLLSVYGPRGS
                     GKTALMEIATGFQRPAAGRVLFDGVDLATLSARELARVHRGGIGWIERGGPQLPDLTV
                     EEYVALALYRDVGRRRAHRRARTALAAYGVEDCAGERWADLSDTARTLCAIAQAMARK
                     PKLLIADDPTAGLGIVDRERIGSVLRTTAERDGVGVLTAVPDMSAMLHAHELLLLSRG
                     RLLAPAERSSKPDATVVQFPDGRRSA"
     gene            complement(12824..>13201)
                     /locus_tag="VHF88_04735"
     CDS             complement(12824..>13201)
                     /locus_tag="VHF88_04735"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX2161110.1"
                     /translation="RALAKGEAPVIRSDGRPERDYLYVEDAVDAYLAVARSLDEAPLR
                     GRAWNAGWGSPLAVREIVDRLIAVAGFDVLPEVQGDGTPHGEIDRQYLDSSAIRAELG
                     WEPRVALDEGLRRSYDWYARLVG"
BASE COUNT         1900 a         4596 c         4772 g         1933 t
ORIGIN      
        1 ggggctcacg ccccccggac cccccgcgga gccatggccg cgtgcggtgc tcatacggct
       61 cgtcatgccg gctggcgctc cgtcggctcg tcggtcggga ggtgctgccc ggcggctgga
      121 ccgccgccct gacccgcggt cagcagctcg tagagcaccg ccatccggac gacgacgccc
      181 gactcgacct gctcggcgat cagcgcctgg ggcgagtcga tcacctcggc agacagctcg
      241 actccgcggt tgaccgggcc gggatgcatc agcagctgcc ccggtccgag ccggcgcccg
      301 ttgatctggt agcgcgccgc gtactcgcgc agcgacggca ccagttcgcc ggtcatgcgc
      361 tcctgctgca tccgcagcgc gtagacgacg tcggcctcac cgagctcgtc gagggtgtgg
      421 cgcacctcgc agccgagcgc ctcgatcccg tgcgggatca gcgtcggcgg cccgcagacg
      481 gtgacccggc agcccattcg cgcgaacgcc agcacgcccg accgtgcgac gcgactgtgc
      541 agcacgtcgc cgacgatcca gacgcgcagg ccgtcgagcg agccggtgcg gcggcgcaac
      601 gtgtagaggt cgagcagcgc ctgggtcgga tgctcgtgct tgccgtcgcc ggcgttgatc
      661 accgacgcgc gcgtccagcg ggcgacgagc cccgccgcac cgacgtgcgg cgaccggatc
      721 acgatcgccg ccgggtcata ggccgacagc gtctgcaccg tgtccttgag cgactcgccc
      781 ttgtcgaccg acgagccctg cgccttgatc gacacgagat cggccgagag ccgcttggcg
      841 gccagctcga acgacgagct cgtgcgcgtc gaggactcgt agaagaggtt gacgaccgtg
      901 cggccgcgca gcgtcgggac cttcttgatc tcgcggccgg agacctcggc gaagctctcc
      961 gcacggcgca ggatccgctc gatgtcctcg cgcgacagcc cgtcgatcga cagcaggtgg
     1021 cgcttcacga gtcggttctc ctgatcgtga tgtcgacgct cgacacgatc gtcacctcgt
     1081 cctcgccgtc gagctcctcg acgtggacgt tgacccgctc gctgcgcgcg gtcgggaggt
     1141 tcttgccgac gtagtcggga cggatcggca gctcgcggtg gccgcggtcg gcgagcacgg
     1201 cgagctggac ccgccgcggc cggccgtagt cgaacagcgc gtcgatcgcc gcgcgcaccg
     1261 tgcgaccggt gaacagcacg tcgtcgacga gcacgacggt cgtctcctcg accgggaagt
     1321 cgacgtgcga ggcgtggacg actgggccgg tcgggtcgtc ggcgccgcgc aggcccacat
     1381 cgtcgcggta gaaagagatg tcgacgtcgc caagcgggac cggctcgccg accagctctg
     1441 ccacttggtc gcgcagccgg gcggccagca gggcgccgcg gcggtggatg ccgacgatcg
     1501 cgagcgggtc gctcccgttg cgctcgacga tctcgtgagc gatgcggacc agcgtccgcc
     1561 gtacgtcatc gcggtcgagg acgaccttgt ggctcactgc gatcttgccc cttcctggcc
     1621 tcacgggacc gggttaaaga gactgcgccg cgaatgtagc aggacgcttg gacggcgacc
     1681 gctactcggc cctgtggtgg accggctcgc cgcgctcggg gaacgggatc tcgattcggt
     1741 cgaagtcacg cgggacgatc gtcggctcga actccgcacg tgcctcgtcg cgcagcggtc
     1801 cgaccgggta gcgggtcgac acgtgggtaa gcgcgagcag cgcgacgtcg gcgtcggccg
     1861 cgagcatcgc cgcctggcgg gcggtcgagt gaccggtctc gcgcgctcgg tcgaggtcct
     1921 cgtgcatgaa cgtcgcctcg tgcacgagca ggtcggcgcg gtgggcctcg ccgcgtgtca
     1981 tgtcgcacgg cgcgctgtcg ccggagaaga cgatcctgcg cccgaggcgc gtctcgccga
     2041 gcacctgccc cggctcgacc tcgcccgacg ccccctggac cgtctcgccg cgttgcagcc
     2101 gcccgaagtc cggtcccggc gtgacgccca gctcgcgcgc ccgcgcctcg tcgaagcggc
     2161 cgggccggtc gtcctcgatc agcgcgtatc cgtgggcggg gacgcggtgg gcgaccgaga
     2221 aggcggcgat cacgtagcca tcacgctcga ggtcctcccc gctctcgagc tcgatcgtcc
     2281 ggacgtcgaa gcccggcttg ccgatcagtg gcgccagcac cctgaagagc tggcgcagcc
     2341 ctggcgggcc gtagacgttc aacggccgct ccctgccctg gagcccgaag gtcttgagca
     2401 tcccgggcag gccgagcacg tggtcggcgt ggaagtgcgt gatgaagacg tcgtcgaggt
     2461 cgacgagccc gaccgaccgg atcagctggc gctgcgtgcc ctcgccgcag tcgatcagca
     2521 gccgctcgcc gccgcggcgg atcagcgtcg cgggcaggcc gcggcgcgcc gtgggcgccg
     2581 agccggccgt gccgatgaac accacgtcga ggtccatgag gctgcgtatc ttcgctggcc
     2641 ggcgaccggg ccgctcagtc gagcgtcacg aggtcgaact cgacgcgtcc gtcgtcgaca
     2701 cgcgccaggc ccatcgtgtg ggccggcgag cgccgccgct cggtcgggct gccggggttg
     2761 aagatctgga agccgtcgga ctcctcgtgc agcgggatgt gcgagtgtcc gaacacgacg
     2821 gcggcggccc gcgggaaccg ccggcgcagg cgcgcgaggc ggcctcgggc aggcccggcg
     2881 tcgtggacga tgcctatctc gacgccgccg gcgtcgaccg tcgcctccgc cgatagcgtc
     2941 cgctgcagct ccgggctgtc gacgttgccg cacacgccga cgaccggcgg acccagcgcc
     3001 tcgagctcgg cgagtacggc gactgtgctc aggtcgccgg cgtggacgat cgtgtctgcg
     3061 ccgcgcagca gctccacgca gcgctccggg agccgccgcg atcccctcgg caggtgcgtg
     3121 tccgagatga tcgcgatcag catcggtcta gacggacgat ctcgctggcg agcgcagctt
     3181 ccggggtggt cggctagtcg gagtacccgt cttggctggc ggaggcagtc gcgctcggct
     3241 cgaggacgaa ggactcgacg gcgatgtcgg gatcgaggtg gttgtcgctc aggaacgccc
     3301 ggaccttgcg gccggtcgcc tgctcgacaa gctcgatcag gtcggtcgcc atcgtccgct
     3361 ggtacgcatg gcgtgcttgg aggacgaggg ttgtctcgcc atctgcgacg aggctgcgct
     3421 cccccttggt gagggtgtcc tccaacacga cggtcacgag atcgtcgctc aggtgggtgc
     3481 gcgcccgcgt ggggccgcgg ccggtgtact ccctgagcag gcgcacgacc ccgttcgaaa
     3541 tagccgacgc ggtaacgcct ccggatggat gctctgccgt ctggctgccg gtcatggcga
     3601 gccgccttcc tccgcctcct tcaaacgagg gctgcgatgc gaagaaggaa acggacgatt
     3661 cgcaggcaac ccgtgatccg gcaaagtgcc cccatgagcg gggtccagcg tcaagccacg
     3721 tcgcacgagt gtagaccctc cggggcctcg tcgcccaacc ggcgcaggca ggtaggtcag
     3781 gcaacggcgc gcagagcgtc gagcacctcg tcggaggcgt cgatcgcctg gcgcatgcag
     3841 tcgtccatgt cgatgtagag gtagttggcg agccgtccgg cgaagaaggt ccggccgtcg
     3901 ggaagctcgg agcgcaggtg gtcctggtag cggttgttga ggctccggtt gcgctcggtc
     3961 tcgatcgggt agtagcgcgt cggcgcattg gtgaactcga acccgaggac ggtgccctcg
     4021 cactgctggc ctgatgcgtg cttggtctcg tggatccgga cgaacgggta gtcgaggctc
     4081 gggtagttga cgaccatcgc gccctgggcg tactcgacgt gcgggatgtg gacgttgcgg
     4141 acggcgatcc cgcgccactc gaggtctccg aaccgcccgc cgcagaagac gtcgagcggg
     4201 caggtgagga cgagcacatc ggcagcatgc tcgcgcagct gctcggcgat gttgccgccc
     4261 gtcacatcga cctctgtgtg gacggtgacc tcgtcgtggt cgagcaggcc gtcgatcagg
     4321 tcggtgtagc cgttcggacc cgccggccag ccctggaaag ggtcggggaa gaggtacggg
     4381 tcgttgtccc agcggaccga gacccgccgc ggagcccact gcgcggacag ctcgcgggcc
     4441 ggacgccccc actgcttctc ggtataggga cggatgtagc gctcgtagag gatcggaccc
     4501 atcaggtcga ggcaccagtc ctcgaagttg gccgcttgag cacgctgctc ggcgtcgagg
     4561 cctttgcgct gctcgagctc ggcgaagatc tcctcgctgc gcgtctggtc ctcgaggtcg
     4621 gacagcagga tcggccagtt gtagatcttg ccctcgacca tgatgtcgac gcggtggcgg
     4681 tagtgtcgaa acggcgtcat ctcgttcgcc aggttccaca cctcctcgtc ctcggtgtgg
     4741 aagatgtgcg agccgtgggg ctcgtagagg acgccgttca tgtggtcgga gcggaccagc
     4801 ccgccgggca cgctgtcgcg ctcgagcagg acggtctcga ccccctcccg cgcgaagcgg
     4861 tgggcaaggg tgcagccggt caagccggct ccgacgacga ggactcgcgg tgcgttgctc
     4921 atgctgcggg ctcctttcgt gcggccccgc catggtcggc acggagcggg atctggtcgt
     4981 cgaagggctc tcctgcgagc gcctgaacca tcaagtggtc catgcgcgcc gcgatcgtgt
     5041 cccagtggtg gatgtgcagc agtggctgga cctttgcgtc gcgctccgcc tgcccgtcct
     5101 cgcgcgtacg ccggcaggcc gctgcgaagc cctccgcgtc gtctgcgaac gccacgacgc
     5161 cgcggtagcc ggcgaccacg tcgggcaccc gcgtcgagac gaccggcagc ccggccgcta
     5221 ggtactcgag cgtcttggtc gggctgatcg agcgggtcgc ctcgttgagc tggaacggca
     5281 tcagagcgat gtcgaagccg ccgagaatct ccggcagcgc ctcgtagggc tggaggccgg
     5341 gatagctgag gttcggcgcc tgcggcacgg ctgcgggatc gatcttggtg accggtccga
     5401 ccatcacgag atcccagtcg ggaagacgct cggcgagctc gccgaccagc gcgaggtcga
     5461 cccgctcgtc gatcacgccg acgtagccgg cgaccggccg ctggcgctcc cgacgccgtg
     5521 agcgggcggc ctggaagtgc tctggcgcca ctccggaggc gaacaggaac gacttgccgt
     5581 ctgcccggtc cttgatcccg tcgtgcagcg agcgaccgcc ggcgagcacc agctgggcct
     5641 cggtcagggt ctggtcctgc aggcgacgca gcagcgggga ggcgtccttg aaggatgcga
     5701 ggtcgtccat gacgtcatag atcaggacgt cggggtccag cgcctgcgcc atcggcagcg
     5761 ccagcggggt gtagagccag accacgcgat cggcgtcccc gagttggtcc cgcagcagcg
     5821 ggccgtagtg ggtcgcgtcg gggtggtcga atccgggcca ggcgttctcg gcgtcgggca
     5881 cctcgagcca tacccgctcg accgatccgt gtggctccga gcgcagcgtc ggctcgtcca
     5941 cctcggtcgt gccgatcggc tcctcgacga acagcgtccg cctgccctgc gcgagcctcg
     6001 acacgatgtg gtgcggccgc tgccacaccc agggccaccg gaggtggctg aacaccacca
     6061 actctgcgtc tgcgctcatg ctgccgtctc ctctcgtgcg cgtgcgacga cgatgcctcc
     6121 gtcgaggaag ccgctctcct cgtcgggcgc gtcgatccag tcgtagtgct ccatctgcgg
     6181 cattaggccg gcgacccact cggcgacgtc gtcggtgaac cggtaggccg gcagttcgct
     6241 cgacgcggcc cccgcggcgg ccatcgcata cgagcgcgac atgctcgacg cgttgcgctc
     6301 cagcgcctcg tcgagccagt agacgccgac tgggtcgatg caacggtcgg cgcgcgcgag
     6361 cagcgagttc cagtcgagcg agtcgatgaa cgggaaccag cagtagccct cgagcggcac
     6421 cccggccgcg acggcgcgct cgcactcgtc gagcgtgtac ttcagccagg tggcgcggtc
     6481 cgcgggcgca ccgcggatgt tcgtctcgct gaagatcatt gggaggtcgt agcggcgggc
     6541 gtactcgatc gcgagcgccg ccgggccgac cggctgcggc gacggggtca cgccctcgat
     6601 gccggacggc tcggggtggc gctctcgtcc cgcgggctcg gaccgccgct ggcgccggaa
     6661 ggcccactcg gagtgggcgt agtagtccaa tccgagcacg tcgacgtggc cgggctcggc
     6721 gtcgagcagg tcggcgccgc cggcctcgac cacgtcgcgc acgaaggggc ggctgccatc
     6781 gccgagcttg ccgagcatgt agtcgagcgc gaagaagcgg cgatcgttcg ccatcgccgc
     6841 gtagccctca ccgcccggct ccagcgcgct gtggccctcg caggtgtcga cgtagacgtg
     6901 acgggcttca ggcagcagct cgcgcacgat cctgctcgcc tgcgcgatcg ccgggagcac
     6961 gttgcgaaac agctcgacga gcggccccat tccacgtccg tacggcggcc agatcgcctc
     7021 gtggccgcag aggaacagcg tcgcgaacgg ctcgttgaag agcgtgtact cctcgatcca
     7081 gggatagcgc cgcgcgaacg cctcgcagta ggcgacgtag gcagggccga agcccgggtc
     7141 gccgaacccc ccgagccagc gcgggtagct cgtgtggtgg acgaggtcga cgatcgggcg
     7201 catgccgttg tcgcgcatcg acccgagcac gcgatcggtc tcggcccagt cgtagacgct
     7261 ccgctccggc tgaacacggt gccaacggac cggatagcgc aggcgggtca cgccgcccgc
     7321 gcgcatcagc tcgaggtccg cgcgccagcg gagcacgtgc tccgagctct cggctacgtc
     7381 gacgtcgtgc gcagggagat aggtactctc gaaggcgccg atgaagggaa ggctcaagcg
     7441 gggatcctcg tcgtcttggg ggcggaggtt ccgctcgccg ccgctggtgg cgacgacggt
     7501 cgttccctac ggggacgggg tcaggagcca cgctcgagtc gcgccttccg acgggcgagc
     7561 gcttacccgg ctccgaggcc gctaaaccgc ggcggagatc cgcgcgagct cgtcgggctc
     7621 gagccgcagc tcgcgcgccg ccgccgagtc gcggatgctc tcgggacggc tggcgccggg
     7681 aatcgggact acctgctcgc cgtgggacag cagccaggcc aaaacgacct gttggaccga
     7741 gacgccgtgc cggtcggccg ccgcgcgcac cgcccctatc cgggaggcgc cgtcggcgct
     7801 gtcgatcccg ccgagcggcg accacgccaa gaaggggatc ccgcgctgct cgcacgcctc
     7861 gacctcgccg ttgtgcagcg gcgccgtgta gtcgagcgac agctcgttct gcacggcggc
     7921 gatctcgacg atcgcctcgg cctctacgag ctgatcgacc gtcacgttcg agagccccgc
     7981 gtggacgatc ttgccggcgt cgcgaagatc tcgcagcgcg ccgaccgact ccgcgaacgg
     8041 gaccttggga tccgggcggt ggagctggta gagatcgatt gactcgacgc cgagcctccg
     8101 cagcgacgcc tcacaggcgg ctttgaggta gtcgggggat ccatcgagct cccaccgccc
     8161 gccgggccgg gtgtgcccac ccttggtggc gacgacgaaa gcgtgctccc gccgacccgc
     8221 gagcgcctgc gcaagcagac gctcgttgtg tccgaagtcg ccctcccccg tcgagtacgc
     8281 atcggcagtg tcgaagagca caacttccgc atcgagcgcg gcatggatgg tctcgcgcgc
     8341 gagaccgcgc tctcttcccc gaccgagcga cagcggcatg caccccatgc cgacccgacc
     8401 gatgtgctcg agggtcctca cgccgaggaa gggcttgccc gcagcaccgg gggcggaaac
     8461 gcagcgtcct tggcgcttcc agcgcaaggg ccctccagcg ccggtggcgg acattcggcg
     8521 atgcgttggg acctttagtt gaaccgcgtc gcccaacgcg aacccctatc ctcgacgggc
     8581 acccgcgccc gaagctcagt ggatagagcg cttgcctccg gagcaagagg tcgctggttc
     8641 gaatccagcc gggcgcggcg cggcggtcac gcggcgcgca ggacgtactt cgcgaagatc
     8701 ccgtcttggg agtgctcggc gtcgaggatc tcgaatcccg agcggcggat catcggctcg
     8761 agcagccaag tgtaggtcga gtgctcgtcg cggacgtgct cctcgagctc ggcgcgggtc
     8821 cactcggcct cgacgctgtc tccgccggtg gcgcaccacg cctcgatgcc ctcctcggcc
     8881 tgcgcggggt cgaagtcgta gacgacgtcc cagaggcgga agatgccgcc gacccgcatc
     8941 gtccgccgaa cgcgctcgag cgcgagcgcc ttccagaagt ccggcagatg atgcagcgcg
     9001 aacctcgagt acacgaagtc ggcgggcgct ccgacgtgct cgtaggtaag gaagccggct
     9061 cgggcgatct cgacgttgcc cagctcgaga gcggcgagct tggattccaa ctgcgccagc
     9121 atgaccggcg agacgtcgac cgcgacgacg cggccgcagg ccggggcgac cgcgaccgtg
     9181 aactgtcccg tccccgcgcc cagctctacg aggagcgact cgggtgtcag cccgtgctcg
     9241 gcgagcagcg ccacctcgga cgcggcggcc gcgtcctcct tggcgtcgta gcgcacggca
     9301 tggtcggcgt ccaggttctc ccgcccggca tgggccagct cgtcgacgaa ccagctgggc
     9361 gtgggacggc ggggaccttc ggaggagagg tcggccatcg tgccagccta gaggggcgtc
     9421 gcggcaccgc tcgagacttc gtcggacgag gtgacgaagt ggacctcacg atcgatctgg
     9481 cagagctcga acggccgcca cgccggcccg ttcgggcgca ccaccaccag gcgggtgccc
     9541 tgtccgatcg cgcgccgatg cgtcttgacg accagctcga tccccgacga gtcgcagaac
     9601 gcgatccggc tgaggtcgac gacgacggtc tccggccgtt gcgagagggc gacgtcgacc
     9661 gccctccgaa ccgcgtcgat cgacgagagg tcgatctcgc cgcgcaccgc tacgacatga
     9721 gccgtccctt ggacgctcga ctcgagctcg accagtgacg aagactgcat ggcaactcct
     9781 cgaagacgga attcgtccga gggagccggc gcaccgtgcg atgcgctcgc ccttctcgct
     9841 gtggggcgtg agcggcgcgg cgcctgtgtc cgcgaagccc gcggcaacgt tagcgcgagc
     9901 cggccggcat ggctagaccc tcgtgctcga gcccgccggc gacgagcccc cgagcgagaa
     9961 cggcgcagcg actgagtagg cggctcagcc gagcggcttg cgctccctgt cgccgccggg
    10021 ctgcaccggc gactcgtagc gcgtgacgac gtcggtaccg gcgtagagcg cgaggaagcg
    10081 caacggctcg tcgccggcgt tgcgcacctc gtgcggcacg tccttgggaa cgagcgccac
    10141 gccgtttgcc gcgaccgcca tcggagagtc cccgaccgtc acctcggcct gaccggcgag
    10201 cacgacgatg acctcctcgg cgctgtcggt gtggcgtgga aggcggcagc cgcgtccgac
    10261 ctcgagcagc gagctcccgg tggagcgcgc gccgtgctcg ggggtgtgcg cggaggccga
    10321 gcgccacgcc gcggactcgt cgccgtcgat ccaggcgtcc tccagctcca gctcgtcgag
    10381 ggggatcgag gccatcgatc ggcgcttacc cggacccgcg ccgcgctgaa ccgcggccgc
    10441 tagcgcaggg agtcgggcgc cggcatgcgc gtcagcagcc gccggtagcg cttcatcgcg
    10501 gcaggcgcga agtcgccgtg cgtctggcgt cgtaggcggg cgacccgctc gcggtggaag
    10561 gggctcgaga ggctctcggc atgaccgatg acgttgcgcg tggcgatccg gtggcgcccc
    10621 tggagcatgc gcgtcagcct cagcgaggcg ttcagttgcc ggcggtcagc catgacctgc
    10681 gcgtcactga ccccgacgtg ctcgatcccg atcgccgtcc agttgaggcc gacggtgtgg
    10741 cggcacatga tcgacgtcgg cacgagctgg tggatcgcgc cgtcgcggtc gatcagatag
    10801 tgggcgcaga cgccgggcag ctcgccgagc tcgacgtcgg gcacgttggc cgcaaaggtg
    10861 ttgaaggcgg aggcgaagct gttcgaggcg gtgtagtgct cgacgatcac ccgtggccgg
    10921 cgcaggcgga agtcgtcgat cccgtagtgg cgttgggcgt aggcgcgcat ctcgcgccgc
    10981 cggcgcggcc cgaacgggat cggggtctcg acgacgcgcg gcggcgcgac cgcgcgcgcc
    11041 accgcgatca cggcatccgc gtggcggtcc gcctctcgtc ggccgagagc gccggcgggc
    11101 agctcgacga cgaacgcgct gtcgcgcggg aaggcgtggt tctgccagct cgtcgccgtc
    11161 ccggggagcg gaccgatctc cctgtgggga aggcgcgaac gctcggcgta cagacgctcg
    11221 aggtacgggt cgccgccgga ccggtcgacg agccgcatgt gctggtggta ccagatcgtt
    11281 acccgcgggc gaacgcggcg aatgagccgc atgagcgcag ccgtctccgg ctcggaggcc
    11341 ggccccgcac cggagtggtg ggtgtcgaac ggcgcgccga tcggctccca gcggaacggg
    11401 aaattgcggt tgaggtcgac cccgcgagcg ttccagcgag taccggcgcg accgccatcc
    11461 gggttcgcgc tgtcgacgag cagcagcgcg gtcccgcgcg gcgggcgagc ggccctcaag
    11521 cgtcgcacaa ccgcgcgacc cgccggctcg ctgccgtgga tctcgccgac gacgagcacg
    11581 acgcggtcgg cacgcgccga gccgacccgt cgggcgcgca gcggcgagcc ctgcagcgac
    11641 cggccgacca catccagtgt gctcgctggc gtgactgccg cgcgatcgcc gacgtcggtc
    11701 tcccccgcct tcgcggcacc gcagcccgct gcggcgaggc cggcgaccac gagcgctgtt
    11761 cggccgacgg cgatgacccc catccgacca cagtagaggg gcccggctcg atacctgcgc
    11821 gagagtactc tcgcgcaggt attgcccgcc gcgaatcgac gcactacatt gcgggaacgc
    11881 gtgtgacgga gggccgcggt tcacatcctc gaagctccgt agacggcgag gctcccactt
    11941 tcctccaacg gtggggaggc cggccgggcg cgcaagaggg gaggtcgatg cgctggagcg
    12001 cgccagacgc tcgcgcatcg cacctcccct cggttcgcgg gcgttcgacc ggctagcgcg
    12061 aggagggttt cgttgtcgct gctccgtctg gaatcggtga ccaagtcgta ctggcggggc
    12121 ccgcatgagc agcgcgtgct gcgcggggcg gcgctcgagc tgcgcgccgg gtcgttgctg
    12181 tcggtgtacg gcccacgcgg ctccggcaag accgcgctga tggagatcgc cacgggcttc
    12241 caacgaccgg cggccggcag ggtcctcttc gacggggtgg atctcgccac cctgtcggcg
    12301 cgagagctgg cgcgcgtcca tcgcggcggg atcggctgga tcgagcgcgg cggtccgcag
    12361 ctcccggacc tcacggtcga ggagtatgtc gcgctcgcgc tctatcgcga cgtcggccgg
    12421 cgtcgcgccc accggcgtgc gcggacggcg ctcgccgcgt acggcgtcga ggactgcgcc
    12481 ggcgaacgtt gggccgacct gtccgacacg gcacgcaccc tctgtgcgat agcccaggcg
    12541 atggctcgca agccgaagct gctgatcgcc gacgacccga cggccgggct cgggatcgtc
    12601 gaccgcgagc gcatcggctc cgtcctgcgc acgacggccg agcgcgacgg cgtcggcgtg
    12661 ctgacggcgg tacccgacat gtcggcgatg ctgcacgccc acgagctgct tttgctgagc
    12721 cgcggccgcc tgctcgcgcc ggccgagcgg tcgtccaagc cggacgcgac cgtcgtgcag
    12781 ttcccggacg gacgccgctc cgcctagcgc ggtccgtgag cgctcagccg acgaggcgcg
    12841 cgtaccagtc gtagctgcgg cgcaatccct cgtcgagcgc gacgcgcggc tcccagccca
    12901 gctcggcacg gatcgcgctc gagtcgaggt actggcgatc gatctcaccg tgtggcgtgc
    12961 cgtcgccctg gacctccggc aggacgtcga atccggcgac ggcgatcagc cggtcgacga
    13021 tctcgcgcac cgcgagcggc gatccccagc ccgcgttcca cgcgcggccg cgtagcggtg
    13081 cctcgtcgag cgagcgcgcg accgcgaggt aggcgtcgac ggcgtcctcg acgtaaaggt
    13141 agtcgcgctc aggcctgccg tcggagcgga tcaccggcgc ctcgcccttg gcgagcgcgc
    13201 g
//
LOCUS       DATABV010000112         6645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_836656, whole genome shotgun sequence.
ACCESSION   DATABV010000112 DATABV010000000
VERSION     DATABV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836656"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VHF88_04740"
     CDS             complement(<1..909)
                     /locus_tag="VHF88_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX2161111.1"
                     /translation="MTTSTATQDYKVADIGLADFGRKEISLAEVEMPGLMKMREEFGA
                     SQPLKGARIMGSLHMTIQTAVLIETLTALGAEVRWVSCNIFSTQDHAAAAIAATGVPV
                     FAWKGETLEEYWWCTEQALNWPDGDGPNMILDDGGDATLLVHKGREFEQAGAVPDPAS
                     ADSEEYRIILETLQRSLEEDPQRWTRIAARIKGVTEETTTGVHRLYQFAETGDLLFPA
                     INVNDSVTKSKFDNLYGCRHSLIDGINRGTDVMIGGKVAVVCGYGDVGKGCADSLRGQ
                     GARVIVTEIDPICALQAAMEGYEVKTL"
     gene            complement(957..1319)
                     /locus_tag="VHF88_04745"
     CDS             complement(957..1319)
                     /locus_tag="VHF88_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2161112.1"
                     /translation="MAALTINEAAETTGWSARMLRYIEREGLIEPSRSAAGYRLYGPE
                     ELQRLRTLRELLAEHELSLGDVGFALRMSRDGDLRGAVEAWLAAEPKRPEGVAASDWL
                     RFEQQKHERLLAGAAKAA"
     gene            complement(1354..2325)
                     /locus_tag="VHF88_04750"
     CDS             complement(1354..2325)
                     /locus_tag="VHF88_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX2161113.1"
                     /translation="MIEDVLAQPHQIGDALWRVEAAGIAPRDMSGGLVVCGMGGSAVG
                     GDLAAAAIGGRARRAIRTVRGYELPPDVGPDTLVLCASYSGSTEETLACFEAAEQAGA
                     QLVVLTTGGPLAERARAAGVPVAGIPSGMQPRAAVVYMTIGALECAALCGAAPSLRDE
                     IEGSREVLEQLAEDSGADSIARALEDTIPIVHGAGPTTAIARRWKTQLNENSKLPAFA
                     SELPEANHNEIEGWRRGLDLAPLSAIFLHSPELHPRLERRMELTGERLSDIGAPVVRV
                     EARGDTPVAHVMSLVMAGDLVSVSLAELEDIDPTPVDAIQRFKAALG"
     gene            complement(2322..3371)
                     /locus_tag="VHF88_04755"
     CDS             complement(2322..3371)
                     /locus_tag="VHF88_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX2161114.1"
                     /translation="MQALVLAGGRGTRLQPLTYTTPKPVMPLAGRPFLTFMLDWLRGH
                     GVNEAILSCGHMSEGVQSVLGDVYGGMRLRYVIEEEPLGTAGPVRLALDEGVLDERLL
                     VLNGDVLTDIDLTAELEQHVETGARCTLALIAVDDTASYGVVPTLPDGRVEAFLEKTT
                     GEVPTNRINAGAYVLEREVVEALVPAGRAVSFEREVFPELVGAGLYGWAAGGYWIDIG
                     TPERYLESTYDLLAGAVESTLPPRDETGSLIGDGCLTSGAHIGPQTVLGTHCSVGSDS
                     SVERSVLHDRVIVGADCVVREAVLAERVQVGDGARIEPGAMIGAGARVAPGAVVGDDA
                     RIEPDAVVEAGETVG"
     gene            complement(3373..6078)
                     /locus_tag="VHF88_04760"
     CDS             complement(3373..6078)
                     /locus_tag="VHF88_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX2161115.1"
                     /translation="MAEKEPIGVIGVGWVGLCTAACFAELGHPVYARDILPEKVESLT
                     RGDVPMHEPGLPELVRSNAERLHFTTDMREVLDNAELLFCCVDTPPTYSGDADLSRVE
                     AVIAELGESTSHAIVMKSTVPVGTGRSIQRRREGLGYVSNPEFLKEGTAVDDFMHPDR
                     VVVGAGNGSDGFAARVAALYEPLGAPIVRTDVASAEMIKLASNAFLATKISFINEIAN
                     VSEELGADVGEVARGMGLDNRIGDKFLKAGLGFGGSCLVGDETVLVRHRGRTQMLTLE
                     TLWERLAAEEAPVDGMVSSASLEVMSWVPGGEAPVFLPVMCVTRRDHDGTLIEVGTKM
                     GRRVRCTPDHPWVVADGRDGELEVKLASELTREDWIPLAQGVERPADAAAVASLVGAA
                     EAAALSPSQVIVRPRREHVDGVLGRSVEERRKVFGERVPVTQRSGDVKRTGLLRLDEV
                     ALAGLSADRTTLKTAKNGAEIASEVCLDRDFWRVAGLYLAEGHATVALPHHRLSWFFH
                     PEREDHLVDEVAAFWLRHGLRVTTPKLATTRAVKVSSRLLTAWWTQVLGMGRNGYEQR
                     LPDLIWDRPDGDKWALLSGLFEGDGSWSLVNGGPSVIIEMGTVSDELADGVLRLLGDL
                     GIAASRRIGRTAKSTKDTHWIRISGAEQVERAIELVPMRDRPGVLISIGRQAKRIAAT
                     GYRRLGDEGPALLRVTSTKEERFSGPVYSLEVPLAHTFVTSGGLISHNCFPKDVSALK
                     QLAGNSGYHFQLLTSVIEVNDLQKRRAVGKLQKHLGSLVGKEIALLGVSFKPDTDDVR
                     EATSLVLAARLQGEGAHVRVYDPVALDNAEEMLGGSRVCGSALEAVDGADAVVLVTEW
                     PEFAELDWAGAVRERMAQPVVVDGRNFLDREALRAAGFTYEGIGR"
     gene            complement(6098..>6645)
                     /locus_tag="VHF88_04765"
     CDS             complement(6098..>6645)
                     /locus_tag="VHF88_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161116.1"
                     /translation="VKKLAQQFLGLEDTKGPRGALKPKGRKRAARPPGDGGLEKEAQF
                     GKDQAIQAIEQRAGGDFPVYYPTVRVAGYEYIGPPRVYKILTPDGKRKRSYRMVLKSP
                     VVGEYFGIQGTRWMDPPILDTPSEKRMIKGREFELHYEGDRLRLVAWRTPNAVYWVSN
                     TLLQTLSERQMLAIARSTRLP"
BASE COUNT          942 a         2384 c         2297 g         1022 t
ORIGIN      
        1 gagcgtcttg acctcgtagc cctccatcgc cgcctgcagc gcacagatcg ggtcgatctc
       61 ggtgacgatc acgcgcgcgc cctggccgcg cagcgagtcc gcgcagccct tgccgacgtc
      121 gccgtagccg cagacgacgg ctaccttgcc gccgatcatc acgtcggtgc cgcggttgat
      181 gccatcgatc agcgagtggc gacagccgta gaggttgtcg aacttcgact tcgtgacgga
      241 gtcgttgacg ttgatcgccg ggaacagcag gtcgccggtc tcggcgaact ggtagagccg
      301 gtggacgccc gtcgtggtct cctccgtgac gcccttgatg cgcgcggcga tccgcgtcca
      361 gcgctgcggg tcctcctcca gcgagcgctg gagcgtctcg aggatgatcc ggtactcctc
      421 ggagtcggcc gaggcgggat ccggcaccgc gcccgcctgc tcgaactcgc ggcccttgtg
      481 gacgagcagc gtcgcgtcgc cgccgtcgtc gaggatcatg ttcggtccgt cgccgtcagg
      541 ccagttcagc gcctgctcgg tgcaccacca gtactcctcg agcgtctcgc ctttccaggc
      601 gaagaccggc accccggtgg cggcgatcgc cgcggccgcg tggtcctggg tggagaagat
      661 gttgcacgag acccagcgga cctcggcgcc gagcgccgtc agcgtctcga tcagcaccgc
      721 ggtctggatc gtcatgtgca gcgagcccat gatccgcgcg cccttcagcg gctggctggc
      781 gccgaactcc tcgcgcatct tcatcaggcc cggcatctcg acctcggcca acgagatctc
      841 cttgcggccg aagtcggcca ggccgatgtc cgcgaccttg tagtcctggg tggcggtgct
      901 ggtggtcact cgttctcctt gatcactgtg atgttcgtca aagtcgttcg cttcgctcag
      961 gcggccttcg cggccccggc gagcagccgc tcgtgcttct gctgctcgaa gcgcagccag
     1021 tccgacgccg cgacgccctc cggccgcttc ggctctgccg ccagccatgc ctcgacggcg
     1081 ccgcgcagat cgccgtcgcg gctcatccgc agcgcgaacc cgacgtcgcc gaggctgagc
     1141 tcgtgctcgg ccagcagctc gcgcagcgtg cgcagccgct gcagctcctc aggcccgtac
     1201 agccgatacc cggccgccga ccgcgacggc tcgatcagcc cctcgcgctc gatgtagcgc
     1261 agcatccgag ccgaccaacc ggtcgtctca gccgcctcgt tgatcgtcaa cgccgccatt
     1321 cgacggccaa ccttaccaag gttgtgttaa ggttcagccg agggcggcct tgaagcgctg
     1381 gatggcgtcg acgggggtcg ggtcgatgtc ctcgagctcg gcgagcgaga cgctgacgag
     1441 gtcgcccgcc atgacgagcg acatcacgtg ggcgaccggc gtgtcgccgc gggcctcgac
     1501 gcggacgacg ggggcgccga tgtcggaaag gcgctcgccg gtcagttcca ttcggcgctc
     1561 gaggcgcggg tgcagctcgg ggctgtggag gaagatcgcg gacaacgggg cgaggtcgag
     1621 gccgcggcgc cagccctcga tctcgttgtg gttggcctct gggagctcgc tggcgaaggc
     1681 cgggagcttg gagttctcgt tgagctgggt cttccagcga cgggcgatcg ccgtggtcgg
     1741 gccggcgccg tggacgatcg ggatcgtgtc ctcgagcgcg cgggcgatcg agtcggcgcc
     1801 gctgtcctcg gcgagctgct cgaggacctc gcgggagccc tcgatctcgt cgcgcagcga
     1861 gggtgcggcg ccgcacagcg ccgcgcactc gagcgcgccg atcgtcatgt agacgaccgc
     1921 ggctcggggc tgcatgccgg acgggatccc ggcgacggga acgccggcgg cgcgggctcg
     1981 ctcggcgagt gggccgccgg tcgtcaggac gaccagttgg gcgccggcct gctcggccgc
     2041 ttcgaagcag gcgagcgtct cctcggtgga gccggagtag ctcgcgcaga gcacgagggt
     2101 gtcggggccg acgtcaggcg gcagttcgta gccgcggacc gtccggatgg cgcgtcgtgc
     2161 acgaccgccg atcgccgcgg cagcgaggtc tccgccgacg gccgagccgc ccatgccgca
     2221 gacgacgagg ccgcccgaca tgtcgcgtgg agcgatgccg gcggcctcga cgcgccagag
     2281 ggcgtcaccg atctggtgcg gctgagcgag gacgtcctcg atcatccgac ggtctccccc
     2341 gcctcgacga cggcgtccgg ctcgatccgc gcgtcgtcgc ctacgaccgc tcccggcgct
     2401 acccgtgcgc cggcgccgat catcgctccc ggttcgatcc gcgctccgtc gccgacctgg
     2461 acccgctcgg cgagcaccgc ctctcgaacc acgcaatccg cgccgacgat gacccggtcg
     2521 tgcagcaccg agcgctcgac cgacgagtcg ctgccgaccg agcagtgggt cccgaggacc
     2581 gtctgcgggc cgatgtgcgc accgctggtc aggcagccgt cgccgatcag cgagccggtc
     2641 tcgtcgcgcg gcggcagcgt cgactcgacg gctccggcga gcaggtcgta ggtcgactcg
     2701 aggtagcgct cgggagtgcc gatgtcgatc cagtagccgc cggccgccca gccatagagc
     2761 cccgcgccga ccagctccgg gaacacctcg cgctcgaacg agacggcgcg cccggctggg
     2821 acgagcgcct cgacgacctc gcgctcgagc acgtaggcgc cggcgttgat ccggttggtc
     2881 gggacctcgc cggtcgtctt ctcgaggaac gcctcgacgc ggccgtccgg gagcgtcggc
     2941 acgacgccgt agctcgcggt gtcgtcgacc gcgatcagcg cgagcgtgca gcgtgcgccg
     3001 gtctcgacgt gctgctcgag ctcggcggtc aggtcgatgt cggtcaggac gtcgccgttg
     3061 agcaccagca gccgctcgtc gagcacgccc tcgtcgagcg ccaggcggac gggaccggcg
     3121 gtgccgagcg gctcctcctc gatcacgtag cgcagccgca tcccgccgta gacgtcgccg
     3181 agcaccgact ggacgccttc ggacatgtgg ccgcaggaga ggatcgcctc gttgacgccg
     3241 tggccgcgca gccagtcgag catgaaggtc aggaacggcc gcccggccag cggcatcacc
     3301 ggcttcgggg tggtgtaggt cagcggctgc aaccgcgtgc cccgaccgcc cgcgagcacc
     3361 agtgcctgca cgtcagcggc cgatgccctc gtaggtgaag ccggccgcgc gcagcgcctc
     3421 gcgatcgagg aagttgcgcc cgtcgacgac gaccggctgc gccatccgct cgcggaccgc
     3481 gccggcccag tcgagctcgg cgaactcggg ccactcggtg acgagcacga ccgcgtcggc
     3541 gccgtctacc gcctcgaggg cggatccgca gacgcgggag ccgccgagca tctcctccgc
     3601 gttgtcgagg gcgacgggat cgtagacgcg cacgtgggcg ccctctccct gcagccgggc
     3661 cgcgagcacc aggctcgtcg cctcgcgcac gtcgtcggtg tcgggcttga acgacacccc
     3721 gagcaacgcg atctccttgc cgacgagcga gccgaggtgc ttctgcagct tgccgaccgc
     3781 ccgccgcttc tggaggtcgt tgacctcgat gaccgacgtc agcagctgga agtggtagcc
     3841 gctgttgccc gccagctgct tgagcgcgct gacgtccttg ggaaagcagt tgtgcgagat
     3901 gaggccgccg ctcgtgacga aggtgtgagc gagcgggacc tcgagcgagt acaccgggcc
     3961 ggagaaccgc tcctccttgg tcgacgtcac ccgcaggagc gcggggcctt cgtcgccgag
     4021 ccggcgatag cccgtcgcgg cgatgcgctt cgcctggcgt ccgatggaga taaggacgcc
     4081 cgggcgatcg cgcatcggca cgagctcgat cgcccgctcg acctgctccg ccccgctgat
     4141 ccggatccag tgggtgtcct tggtcgactt cgccgtccgg ccgatccggc gcgaggcggc
     4201 gattccgaga tcgccgagca atcgcaggac cccgtcggcc agctcgtcgc tcacggtgcc
     4261 catctcgatg atcacgctcg gaccgccgtt gaccagcgac cacgacccgt cgccctcgaa
     4321 gaggccggac agcagcgccc acttgtcgcc gtcgggccga tcccagatca agtcgggcag
     4381 gcgctgctca tagccgttgc ggcccatgcc cagtacctgc gtccaccacg cggtgagcag
     4441 tcgcgaggac accttcacgg cgcgggtggt ggcgagcttg ggcgtcgtca cccgaagtcc
     4501 gtgccgtagc cagaaggccg ccacctcgtc gaccaggtgg tcctcgcgct cgggatggaa
     4561 gaaccagctc agccggtggt gcgggagtgc cacggtggcg tggccctcgg cgaggtagag
     4621 gccggccacc cgccagaagt cgcggtcgag gcagacctcg ctggcgatct ccgctccgtt
     4681 cttcgccgtc ttgagcgtcg tgcgatccgc cgacaggccc gccagcgcaa cctcgtcgag
     4741 tctcaggagg ccggttcgct tcacgtcccc cgagcgctgc gtcacgggca cccgctcgcc
     4801 gaagaccttc cgtcgctcct ccaccgagcg ccccaggacc ccgtccacgt gctcccgacg
     4861 cggcctgacg atcacctggc tgggagacag tgccgccgct tcggcggccc ccacgagcga
     4921 ggcgaccgcg gccgcgtccg ccggacgctc gactccctgg gcgagcggga tccaatcctc
     4981 gcgcgtcagc tcggaagcga gcttcacctc gagctcgccg tcccggccgt cggcgacgac
     5041 ccatggatga tccggcgtgc agcgaacgcg gcgccccatc ttcgtgccga cctcgatcaa
     5101 ggtcccgtca tggtcgcgcc gcgtcacgca catcactgga aggaagaccg gcgcctcgcc
     5161 cccggggacc cacgacatga cctccaggct cgccgacgac accatgccgt cgacgggcgc
     5221 ctcctccgcc gcaagccgtt cccacaaggt ctccaacgtc agcatctgcg tccggccgcg
     5281 gtggcggacg agaaccgtct cgtcgccgac gaggcagctt ccgccaaacc ccagtcccgc
     5341 cttgaggaac ttgtcgccga ttcggttgtc gaggcccatg ccgcgcgcga cctcgccgac
     5401 gtcggcgccg agctcctcgg agacgttggc gatctcgttg atgaacgaga tcttggtcgc
     5461 gaggaaagcg ttcgaggcga gcttgatcat ctcggcgctg gcgacgtcgg tacggacgat
     5521 cggggcgccg agcggttcgt agagggcggc gaccctagcc gcgaagccgt ccgacccgtt
     5581 gccggcgcca accacgacgc ggtcgggatg catgaagtcg tcgaccgcgg tgccctcctt
     5641 gaggaactcc gggttcgaga cgtagccgag gccctcgcgg cggcgctgga tcgagcgccc
     5701 ggtccccacc ggcaccgtgc tcttcatgac gatcgcatgg gaggtggact cgccgagctc
     5761 cgcgatgact gcttcgacgc gcgagaggtc ggcgtcgccg gagtaggtgg gcggggtgtc
     5821 cacgcagcag aacagcagct cggcgttgtc gagcacttct cgcatgtcgg tcgtgaagtg
     5881 gagccgctcg gcgttgctac gcaccagctc gggcaggccc ggctcgtgca ttgggacgtc
     5941 gccgcgcgtc agcgactcca ccttctcggg caggatgtcg cgcgcataga ccgggtggcc
     6001 cagctccgcg aagcacgccg cggtgcagag ccccacccag ccgacgccga tgacgccgat
     6061 cggttccttc tcagccatag cgaggcgagg caggctacta gggcaatcgg gtcgaccggg
     6121 cgatcgcgag catctggcgc tcgctcaacg tctgcagcag ggtgttcgac acccagtaga
     6181 cggcgttcgg cgtccgccac gcgacgaggc gcagacggtc tccctcgtag tgcagctcga
     6241 actcgcggcc cttgatcatg cgcttctccg aaggggtgtc gaggatcggc gggtccatcc
     6301 agcgcgtgcc ctggatcccg aagtactcgc cgaccaccgg cgacttgagg accatcctgt
     6361 agctgcgctt gcgcttcccg tcgggcgtga ggatcttgta gacccgcggc gggccgatgt
     6421 actcgtagcc cgcgacccgg accgtcgggt agtacacggg gaagtcgccg cccgccctct
     6481 gctcgatcgc ctggatcgcc tggtccttgc cgaactgagc ctccttctcc aagccgccgt
     6541 cgcccggcgg tcgcgcggcg cgcttgcgtc ccttcggctt caaagcgccg cgcgggccct
     6601 tggtgtcctc gagcccgagg aactgttggg cgagcttctt gaccg
//
LOCUS       DATABV010000113         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1078317, whole genome shotgun sequence.
ACCESSION   DATABV010000113 DATABV010000000
VERSION     DATABV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078317"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1070)
                     /locus_tag="VHF88_04770"
     CDS             complement(45..1070)
                     /locus_tag="VHF88_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2161117.1"
                     /translation="MSKRTLVLGSALIALLVAGAFVGIVIATSGDDDDLVVFSARSHY
                     GEEKPFETFDARQDISTRIFGGDAPELYERLRSESDETDADVLVTVDAANLWRAEQAG
                     LLRPVRSPALERSVPAELRDPRGRWYGLTVRARTIMRSVDRVASGAVTTYAGLGDPRW
                     KGRLCLRSGTSDYNISFVADRIAKDGEPATRRMLERWMANDPTILGSDVDVLNAIAKG
                     DCDLGLTNSYYLGRELEEDPDFPVAPIWADQQGRGTHVNLSGIAVVAATDKPRLARQL
                     IDHLGSSEQQQVLAANNHEFAVDPGVEPTDEIAQFGRFKRDPIDVAGAGSHLGDAVRL
                     MNQVGWN"
     gene            1173..>1724
                     /locus_tag="VHF88_04775"
     CDS             1173..>1724
                     /locus_tag="VHF88_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX2161118.1"
                     /translation="MATPDKTRIAKPSPIVEDDQGQLVAKKGVSREVIQELSKIKGEP
                     DWMAQLRLKSYEIFERKPIPTWGVDLSDLDLDELVLYSPPEAGRFDSWDDVPEEMKKT
                     YEDLGIPQAEREHLAGVVGVWRQEPVYEGLKKEYADLGILFCSMDTAIVEYPELVQEY
                     FMKKCVPPQDNKFSALHGAVWSGG"
BASE COUNT          272 a          578 c          621 g          253 t
ORIGIN      
        1 tggcaggcgc ggggcgtaga accgccgcgc gcgcagagcg ctcgtcagtt ccaccccacc
       61 tggttcatca gccgcaccgc gtcgccgagg tggctgcccg cgccggcgac gtcaatcggg
      121 tcgcgcttga agcggccgaa ctgggcgatc tcgtcggtcg gctcgacgcc ggggtcgacg
      181 gcgaactcgt ggttgttggc ggcgagaacc tgctgctgct cggacgagcc gaggtgatcg
      241 atcagctggc gcgccagccg cggcttgtcg gttgccgcga cgaccgcgat gcccgagagg
      301 ttgacgtggg tgccgcgccc ctgctgatcg gcccagatcg gcgcgaccgg gaagtcggga
      361 tcctcctcga gctcacggcc gaggtagtac gagttcgtca ggccgaggtc gcagtcgccc
      421 ttggcgatcg cgttcaggac gtcgacgtcg gagccgagga tcgtcgggtc gttggccatc
      481 cagcgctcga gcatccgccg ggtggccggc tcgccgtcct tggcgatcct gtcggcgacg
      541 aacgagatgt tgtagtcgct ggtcccggag cgcaggcaga ggcggccctt ccagcgcgga
      601 tcgccgagcc cggcgtaggt cgtcacggcc ccggacgcga cgcgatccac ggagcgcatg
      661 atcgtgcgcg cccgcacggt caggccgtac cagcggccgc gtgggtcgcg cagctccgcc
      721 ggcacgctgc gctcgagcgc cggcgatcgg accggccgca gcaggccggc ctgctcggcg
      781 cgccagaggt tcgcggcgtc gacggtgacc aggacgtcgg cgtcggtctc gtcggactcg
      841 ctgcgcaggc gctcgtagag ctccggcgcg tcgccgccga agatgcgggt gctgatgtcc
      901 tggcgcgcgt cgaacgtctc gaacggcttc tcctcgccgt agtgggagcg ggcgctgaag
      961 acgacgaggt cgtcgtcgtc gccggaggtg gcgatcacga tgcccacgaa ggcgcccgcc
     1021 acgagcaggg cgatcagggc cgagcctagg accagagtgc gtttggacat gaccgcttag
     1081 gctagcctaa tacgggtcgg ccggcctgcg cgcccgatac actggagggc cgtgcctccc
     1141 caggcggacg tgaccccgag caacaggatc taatggccac tccagacaag acgcgcatcg
     1201 ccaagcccag cccgatcgtc gaggacgacc agggacagct ggtcgcgaag aagggcgtct
     1261 cgcgcgaggt gatccaggag ctctccaaga tcaagggaga gcccgactgg atggcccagc
     1321 tgcggctgaa gtcctacgag atcttcgagc gcaagccgat cccgacctgg ggcgtcgacc
     1381 tctccgacct cgacctcgac gagctggtgc tctacagccc gcccgaggcc ggccgcttcg
     1441 actcgtggga cgacgtcccg gaggagatga agaagaccta cgaggacctt ggcatcccgc
     1501 aggccgagcg cgagcacctc gccggcgtcg tcggcgtctg gcgccaggag cccgtctacg
     1561 aggggctcaa gaaggagtac gccgacctcg ggatcctctt ctgctcgatg gacaccgcga
     1621 tcgtcgagta ccccgagctg gtgcaggagt acttcatgaa gaagtgcgtc ccgccgcagg
     1681 acaacaagtt ctcggcgctg cacggcgcgg tctggtccgg cggc
//
LOCUS       DATABV010000114         4588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1158330, whole genome shotgun sequence.
ACCESSION   DATABV010000114 DATABV010000000
VERSION     DATABV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158330"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1652)
                     /gene="gcvPB"
                     /locus_tag="VHF88_04780"
     CDS             complement(138..1652)
                     /gene="gcvPB"
                     /locus_tag="VHF88_04780"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX2161119.1"
                     /translation="MSAVLDPRADMQAETIYEKSVVDRRAFTPPALDVPELPIDDLLP
                     SHLRRKRPAELPEISEPEIVRHYNGLSKKNFDLDSGFYPLGSCTMKHNPKLHERVAAL
                     PGHARLHPLQDAEHAQGALELMWNLQQALGEIAGLPHVSLQPSAGSHGELAGLLVTRA
                     YHEDRGEHRHKVLTPDTAHGTNPATVTMAGYEVVKVGTDEHGGVDLEDLRAKATDDVA
                     CLMLTNPNTLGLFDRNIEEIASIVHEVGATLYYDGANLNAVMGISRPGDMGFDIVHYN
                     LHKSFTQPHGGGGPGAGPIAVSNRIEPYIPRPQIVREQAHNGHGPRFALDFDRPKSIG
                     KLRGFQGNFGVFVRSYAYIRSLGGEGLRGASETAVLNANYLRALLAAPDVAEQLPIAF
                     DRICMHEFVLSGRGAKEKLGIKTLDIAKRMLDHGVHPPTVYFPLLVDEALMIEPTETE
                     TKETLDRFAEIVRSILVEAFEDPEIARNAPYTTPVRRLDEAGAAKNPVVRQPPA"
     gene            complement(1649..2995)
                     /gene="gcvPA"
                     /locus_tag="VHF88_04785"
     CDS             complement(1649..2995)
                     /gene="gcvPA"
                     /locus_tag="VHF88_04785"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX2161120.1"
                     /translation="MSHYTSATDADRQAMLDAIGVGSVEELFAEIPEAVRLRRPLDLP
                     AGMAEQDVHEHLAALAARNTSAEDETTFLGAGMYDHYTPALIDSILQRSEFLTPYTPY
                     QPEISQGGLQVMFEYQTAISELTGLPVSNASVYEGPSAVAAAGYLAKLETRRTKLVVS
                     RGLHPHSRETLATHAKGFGMTVAEVGLDGEGATELDALAALVDDDTAAVFVQQPNFLG
                     TVEELGTLGEAAKRTGALFVVAADPLPLGILEPPGALGADVCVGEGQTLGNRLDFGGP
                     SFGFFAAAERFIRKLPGRIAGETRDVDGKRGFVLTLQTREQHIRREKATHNICTAQAL
                     NALAGVIYLSWLGKQGLVELGELMLRRTHYARETLGLEPINPGPVVREFAVRVPDLDA
                     AVARARAAGINPGYALGRDYPEYADGLLIALTERRTKGDIDALASVLGAVREEASV"
     gene            complement(3018..3401)
                     /gene="gcvH"
                     /locus_tag="VHF88_04790"
     CDS             complement(3018..3401)
                     /gene="gcvH"
                     /locus_tag="VHF88_04790"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX2161121.1"
                     /translation="MPASYPEDLKYHAEHDWARIDADIATFGVTWYAQDALGEVVFYD
                     PPALGATVTKDSAYAEVESVKAVSDVFAPLSGEIVEVNEALAESPELINQDPYGDGWL
                     VKVRLSDPSEVDQLLDVAAYRDLLS"
     gene            complement(3417..4496)
                     /gene="gcvT"
                     /locus_tag="VHF88_04795"
     CDS             complement(3417..4496)
                     /gene="gcvT"
                     /locus_tag="VHF88_04795"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX2161122.1"
                     /translation="MPSAAETLRQTPLHGRHQAAGARLVPFAGWEMPVQYEGVRQEHL
                     AVREAAGIFDVSHMGQVETSGPDAEAFLQRVLSNDVSKLAPGGAQYSLLCREDGGVLD
                     DLFTYRLPGSEPRFLTVTNASNHARDFEWLAEHAAGFDVEVVDVADRYAMLAVQGPRA
                     RAAVERIASGALPARMRTTTVAVAGVDCLVCGTGYTGEDGVEVLAPPERAGEIWDALL
                     VEGVVPAGLGARDTLRLEACFHLYGNDLSLDRNPLEAGLAWACKLETGFIGADALRDS
                     KPAQLLVPFAFTGPGIPRQGNPVETTHGDGVVTSGSLSPCLEIGVGMAYLPAAAATPG
                     TPIEVDVRGKRRAAAVRDKPLYKKP"
BASE COUNT          651 a         1545 c         1668 g          724 t
ORIGIN      
        1 attgcggtcg cggcaaccgc cgcgacgacg agcactacga cggctttccg catcagctgg
       61 cccctcccat gggatccctc cggtcctccc ccgcccactc cgcgggcaaa caatgtctac
      121 cacgctcgcg gcgggggtca cgccggcggc tggcgcacca ccgggttctt agccgccccc
      181 gcctcgtcca gcctccgcac cggcgtcgtg tacggcgcgt tgcgcgcgat ctccggatcc
      241 tcgaaggcct cgaccagtat cgagcgaacg atctccgcga accggtcgag cgtctccttg
      301 gtctcggtct cggtcggctc gatcatcagc gcctcgtcga ccagcagcgg gaagtagacg
      361 gtcggcggat ggacgccgtg gtcgagcatt cgcttggcga tgtcgagcgt cttgataccg
      421 agcttctcct tggcgccgcg gcccgagagc acgaactcgt gcatgcagat gcgatcgaag
      481 gcgatcggca gctgctcggc gacgtcgggc gccgcgagca gcgcacgcag gtagttggcg
      541 ttgaggacag cggtctcgga cgccccgcgc aacccctcgc cgccgagcga gcggatgtag
      601 gcgtagctgc gcacgaagac gccgaagttg ccctggaagc cgcgcagctt gccgatcgac
      661 ttcggccggt cgaagtcgag cgcgaagcgc gggccgtggc cgttgtgggc ctgctcgcgg
      721 acgatctgcg gtcgcgggat gtagggctcg atgcggttcg acaccgcgat cggaccggcg
      781 cccggcccgc cgcccccgtg cggctgggtg aacgacttgt gcaggttgta gtggacgatg
      841 tcgaagccca tgtcgccggg ccgcgagatg cccatgaccg cgttgaggtt ggcgccgtcg
      901 tagtacagcg tcgcccccac ctcgtggacg atcgaggcga tctcctcgat gttgcggtcg
      961 aagagcccga gcgtgttcgg gttggtcagc atcaggcagg cgacgtcgtc ggtcgccttg
     1021 gcgcgcaaat cttctaggtc gacgccgcca tgctcgtcgg tgccgacctt gaccacctcg
     1081 tagccggcca tcgtcaccgt cgccggattg gtgccgtggg cggtgtcggg ggtcagcacc
     1141 ttgtggcgat gctcgccgcg atcctcgtgg taggcccgcg tcacgagcag gccggcgagc
     1201 tcgccgtggg agccggcgct cggctggagc gagacgtgcg gcagcccagc gatctcgccg
     1261 agcgcctgct ggaggttcca catcagctcg agcgccccct gggcgtgctc ggcgtcctgc
     1321 agcggatgca ggcgggcgtg gccgggtagc gccgcgacgc gctcgtgcag ctttggattg
     1381 tgcttcatcg tgcacgaccc gagcgggtag aagccgctgt cgagatcgaa gttcttcttc
     1441 gacaggccgt tgtagtggcg cactatctcg ggctcggaga tctccgggag ctcggcgggg
     1501 cgcttgcggc gcaggtgact cggcagcagg tcgtcgatcg gcagctcggg aacgtcgagc
     1561 gccgggggag tgaacgcgcg gcggtccacg accgacttct cgtagatcgt ctcggcctgc
     1621 atgtccgctc tgggatcgag gacggcgctc atacggacgc ctcctcgcga accgcaccga
     1681 ggaccgacgc caaggcgtcg atgtcgccct tcgtacggcg ctcggtcagc gcgatcagca
     1741 ggccatccgc gtactccgga tagtcacggc cgagcgcgta gccggggttg atcccggcgg
     1801 cgcgggcccg tgcgacggcg gcgtcgaggt ccggaacgcg gaccgcgaac tcgcgcacga
     1861 ccggtccggg gttgattggc tcgaggccga gcgtctcgcg cgcgtagtgg gtgcggcgca
     1921 gcatcagctc gccgagctcg acgaggccct gcttgcccag ccacgatagg tagatcaccc
     1981 cggcgagcgc gttgagcgcc tgggcggtgc agatgttgtg cgtcgccttc tcgcgccgga
     2041 tgtgctgctc gcgcgtctgc agcgtcagga cgaagccgcg cttgccgtcg acgtcgcgcg
     2101 tctcgccggc gatccgcccg ggcagcttgc ggatgaaccg ctcggcggcg gcgaagaacc
     2161 cgaaggacgg cccgccgaag tcgagccggt tgccgagcgt ctgtccctcg ccaacgcaga
     2221 cgtcggcgcc gagcgcaccg ggcggctcga ggatcccgag cggcagcggg tcggccgcaa
     2281 cgacgaacag cgcgccggtg cgcttcgccg cctcgccgag cgtgccgagc tcttcgacgg
     2341 tgccgaggaa gttcggctgc tgcacgaata cggcggcggt gtcgtcgtcg accagcgccg
     2401 cgagcgcgtc gagctcggtg gcgccctcgc cgtcgagccc gacctccgcg acggtcatcc
     2461 cgaagccctt tgcgtgggtt gcgagcgtct cgcgcgagtg cgggtgcagt ccgcgcgaga
     2521 cgacgagctt ggtccgccgc gtctcgagct tcgccagata gcccgccgcg gcaaccgcgg
     2581 acgggccctc gtagaccgac gcgttcgaca ccggcaggcc ggtcagctcg gagatcgcgg
     2641 tctggtactc gaacatcacc tgcagcccgc cctgcgagat ctccggctgg tagggcgtgt
     2701 agggagtcag gaactccgac cgctggagga tcgagtcgat cagcgccggc gtgtagtggt
     2761 cgtacatccc ggcgccgagg aaggtcgtct catcctcagc gctggtgttg cgcgcggcga
     2821 gcgcggccag gtgctcgtga acgtcctgct cggccatgcc ggcgggcagg tcgagcggcc
     2881 gccgcaatcg caccgcctcg gggatctccg cgaacagctc ctcgacggac ccgacgccga
     2941 tcgcgtcgag catcgcctgg cggtcggcgt cggttgcgga ggtgtagtgg ctcatcggga
     3001 gggaggaggt cgatctacta ggacagcaga tcgcgatagg cggccacgtc gagcagctgg
     3061 tcgacctcgc tcgggtcgct cagcctcacc ttgactagcc agccgtcgcc gtagggatct
     3121 tggttgatca gctcggggct ctcggctagc gcctcgttga cctcgacgat ctcacccgag
     3181 agcggcgcga agacgtcgga gaccgccttg accgactcca cctcggcgta ggcagagtcc
     3241 ttggtgaccg tcgctccgag cgccggcggg tcgtagaaga cgacctcgcc gagcgcgtcc
     3301 tgcgcgtacc aggtgacgcc gaaggtcgcg atgtcggcat cgattcgtgc ccagtcgtgc
     3361 tcggcgtggt acttgaggtc ctcggggtag ctggctggca ctcgcgcttt ctcctcctag
     3421 ggcttcttgt aaaggggctt gtcacggacc gccgcggcgc ggcgcttgcc acgtacgtcg
     3481 acctcgatcg gcgttcccgg ggtcgccgct gccgctggca ggtaggccat cccgacgcca
     3541 atctcgagac agggcgacag gctgccgctc gtgacgacgc cgtcgccgtg agtggtctcg
     3601 accgggtttc cctggcgtgg gatcccagga ccggtgaacg cgaacgggac gagcagctgc
     3661 gccggcttgg agtcacgcag cgcatcggcg ccgatgaagc cggtctcgag cttgcatgcc
     3721 caggcgaggc cggcttcgag cgggttgcgg tcgagcgaga ggtcgttccc gtagaggtgg
     3781 aagcacgcct cgaggcgcag cgtgtcgcgt gcgccaaggc cggccgggac gaccccttcg
     3841 acgagcaggg cgtcccaaat ttcgccggcg cgctccggcg gggccaggac ctcgacaccg
     3901 tcctcgccgg tgtagcccgt gccgcagacg aggcaatcga cgccggcgac cgccacagtc
     3961 gtggtgcgca tgcgcgccgg cagggccccg gaggcgattc gctcgaccgc ggcgcgtgcg
     4021 cgggggcctt ggacggcgag catcgcgtag cggtcggcca cgtcgacgac ctcgacgtcg
     4081 aagccggcgg cgtgctcggc gagccactcg aagtcgcgcg cgtggttgga ggcgttggtg
     4141 acggtcagga agcgcggctc gctacccggc aggcggtagg tgaagaggtc gtcgagcacg
     4201 ccgccgtcct cgcggcagag cagcgagtac tgagcgccgc caggcgccag cttggacacg
     4261 tcgttggaga gcacgcgctg caggaacgcc tcagcgtccg ggccgctggt ctcgacctgt
     4321 cccatgtggg agacgtcgaa gatcccggcg gcctcgcgca cggcgaggtg ctcctggcgc
     4381 acgccctcgt actgcaccgg catctcccag cccgcgaacg ggaccagccg cgcgccagcc
     4441 gcctgatggc ggccgtgcaa cggcgtctga cggagcgttt cggcggcgga gggcacgccg
     4501 gctggaggct acctgccggc gcggccggcc caccgacgcg cgggcgctgc gtttgaaata
     4561 ggtccgggga tcggcgtaag ttcagctg
//
LOCUS       DATABV010000115         5459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1000699, whole genome shotgun sequence.
ACCESSION   DATABV010000115 DATABV010000000
VERSION     DATABV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000699"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..503
                     /locus_tag="VHF88_04800"
     CDS             60..503
                     /locus_tag="VHF88_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2161123.1"
                     /translation="MIGSIVVGTDGSDTAAEAVRQATELARAIGARIHLVSAYEPVPE
                     SRLRAERLEVPPDLQWTVNPREDVERLLEQEAGEMRAAGVEVETLAREGDPADAILDV
                     AEQRNADLIVVGNKGMTGAKRFLLGSVPNKVSHHAPCSVLIIRTS"
     gene            complement(500..1768)
                     /locus_tag="VHF88_04805"
     CDS             complement(500..1768)
                     /locus_tag="VHF88_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2161124.1"
                     /translation="MPRASFLFATLATLALMAAVAAGPASAAPAYVPGELIVKREGAS
                     SSTTVSVAPGESVKRAAAELDDRRGVAYARPNYIARASAYTPNDPGFPLQWNLATSAA
                     GINMPDAWATARSLRRPGGRNVVIAVLDTGVAYQRFRRFRRAPDLNRFVRGYDFVAGD
                     RHPNDQNGHGTHVAGTIAQSTGNGVGTAGIAYRARIMPVRVLDASGAGDTLAISRGIR
                     FAARRGADVINLSLEFDAGVRAAQIPDIVSALTYARRRGAIVIAAAGNQGSPQVAYPA
                     RARRVMAVAATTARGCQAEYSNSGVHVDIAAPGGGADAANTDTPYDVAICRPGVGGGE
                     FIYQQTFTGSVARFGLPPGYEGTSMAAPHVSGVAALLIGSGQLGPDPGPVAVERRLKS
                     SARDIGSTGWDPRYGWGLLDAAAALREPAG"
     gene            1863..2969
                     /locus_tag="VHF88_04810"
     CDS             1863..2969
                     /locus_tag="VHF88_04810"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2161125.1"
                     /translation="MRTCVTGATGFVGGHLTRELIERGHDVRVTYRDAARLERLRGFD
                     PEPVRADILDRAAMRRAVRGCDVVFHTAGIVASKPVSYVQDINALAPRIAVEAAAAEG
                     VPRVVVTSSVAGIGPVPRGQVGREEDLYRGGGLGLTYPDAKHEGEMEAFAAGARLGVE
                     VVVTNPAYVLGVPLDRRQPGETSTRIIGNYLRGRLPAVVDGGTTIVDVRDVADGHLLA
                     AERGKSGERYVLGGHNTSWPELMAKVARLAGVDYPLAVFPHGVGALARRAEALRLPLA
                     ISAEAMVLMEQNWCYSSAKAKRELGYRPRSHDSTVRATVDWYRELIDAGVLDGGSRSP
                     LSLGAAGLRLADRVGALAVVDALGRRTGHRLVAR"
     gene            3020..3484
                     /gene="tadA"
                     /locus_tag="VHF88_04815"
     CDS             3020..3484
                     /gene="tadA"
                     /locus_tag="VHF88_04815"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX2161126.1"
                     /translation="MAAPFFPRDEYFMRLALREAERALEHEDVPVGCAIVLDGEVIAA
                     ARNERELRGDPTAHAEVLALREASRRVGRWRLDGCALYVTLEPCAMCAGAIVLARLPR
                     VVFGATDPKTGAAGSVLDVLAEPRLNHRPQVAGGLLAGESADLLRAFFASRR"
     gene            complement(3497..3985)
                     /locus_tag="VHF88_04820"
     CDS             complement(3497..3985)
                     /locus_tag="VHF88_04820"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX2161127.1"
                     /translation="MRLLFVCLGNICRSPTAEGVMRHLLEREGLGDAVEIDSAGTGDW
                     HVGHAPDERATAAARDRGIALTGQARQVDAADFDAYDLILAMDRANHDDLLALAPDED
                     ARARVRLLREYDPEAVAAGELEVPDPYYGEVDGFEDVLDLVTRACEGLLDAEVRPASR
                     PR"
     gene            complement(3995..4315)
                     /locus_tag="VHF88_04825"
     CDS             complement(3995..4315)
                     /locus_tag="VHF88_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HEX2161128.1"
                     /translation="MRQELRPLFDRVVIKELDPDRVRRSGLVVPPGTNEPPPQHGIVL
                     AVGQGLDWWESVGVKMPVKPGDHVVFPASAGVWIEVEEERLLVCRVGELLGVLDPVED
                     PARV"
     gene            4360..>5459
                     /locus_tag="VHF88_04830"
     CDS             4360..>5459
                     /locus_tag="VHF88_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161129.1"
                     /translation="MTTTTTPGADAYSLPQEFRDFRDTIRDLVQGVVAPRAAEIDATG
                     EYPWDVRKAFADHDLLGLPFPEELGGTGTGTLMLNIAIEEVAKACASSSLILMIQELG
                     TLPIQLHGTDEQKRQWLPRCAAGEWTPAFCLSEPEAGSDPGAMRTTAVRDGDEWVING
                     TKNWITNATIADFYVVFAVTDRENKRFSAFIVEKDREGFSVPKLEHKLGIKGSPTGQP
                     VFEDVRVPADHLIGDEGRGMAVALSTLERTRLGAGAQAVGIAQGATDYAASYAKERIS
                     FGKPIFDHQAIAFKLADMETKTAAARELLYKACAMADRNERGLGKYSSMAKLFASDVA
                     MEVTVEAVQVLGGYGYVNEYPVERMMRDAKIT"
BASE COUNT          744 a         1969 c         2032 g          714 t
ORIGIN      
        1 gcgcccccgc ccccccccca cccccggggt tgcgtggccg gcctgcgatg atcgcggcca
       61 tgatcggctc gatcgtcgtg ggcaccgacg gctccgacac cgccgcggag gcggtgcggc
      121 aggcgacgga gctggcgcgg gcgatcggcg cccgcatcca tctcgtcagc gcctacgagc
      181 cggtgcccga gagccggctg cgcgccgagc ggctggaggt cccgccagac ctgcagtgga
      241 cggtgaaccc gcgcgaggac gtcgagcgcc tcctcgagca ggaggccggc gagatgcgcg
      301 ccgcgggcgt cgaggtcgag acgctagccc gcgagggcga tcccgcggac gcgatcctcg
      361 acgtcgcgga gcagcgcaac gccgacctga tcgtcgtcgg caacaagggc atgaccggcg
      421 cgaagcgctt cctgctcggc tcggtgccga acaaggtctc gcaccacgca ccgtgctcgg
      481 tgctgatcat ccggacgagc tagccggcgg gctcgcgcag cgcggcggcc gcgtcgagca
      541 gtccccagcc gtagcgcggg tcccaaccgg tggagccgat gtcgcgggcg ctgctcttca
      601 gccgccgctc aaccgccacc gggccggggt ccgggccgag ctgaccgctg ccgatcagca
      661 gcgccgccac gccggagacg tggggcgcag ccatcgacgt gccctcgtag cccggcggga
      721 ggccgaacct ggcgacgctg ccggtgaacg tctgctggta gatgaactcg ccgccgccga
      781 ctccgggtcg gcagatggcg acgtcgtagg gcgtgtcggt gttggccgcg tcggctccgc
      841 cgcccggcgc ggcgatgtcg acgtggacgc cgctgttcga gtactcggcc tggcaaccgc
      901 gcgcggtggt tgcggcgacc gccatgacgc ggcgcgcgcg ggccgggtat gcgacctgag
      961 ggctgccctg gttgcccgcc gcggcgatca cgatcgcccc gcgccggcgc gcgtaggtga
     1021 gcgccgagac gatgtcggga atctgcgccg cccggacgcc ggcgtcgaac tcgaggctga
     1081 ggttgatcac gtcggcgccg cggcgggccg cgaagcggat gccgcgcgag atcgccagcg
     1141 tgtcaccggc gccggaggcg tcgagcacgc gcaccggcat gatccgcgcg cggtaggcga
     1201 tccccgccgt gccgacgccg ttgccggtgc tctgggcgat cgtgcctgcc acatgggtcc
     1261 cgtgaccgtt ctggtcgttg gggtggcggt cgccggcgac gaagtcgtag ccgcgcacga
     1321 agcggttgag gtcgggcgcg cggcggaagc ggcggaagcg ctggtaggcg acgccggtgt
     1381 cgagcacggc gatcaccacg ttgcggccgc cgggccggcg cagcgagcgc gcggtcgccc
     1441 aggcatcggg catgttgatc ccggcagcgc tggtcgcgag gttccactgc aacggaaagc
     1501 cgggatcgtt cggggtgtag gcgctcgcgc gcgcgatgta gttcggtcgc gcgtaggcga
     1561 cgccgcgacg gtcgtcgagc tcggccgcgg cccgcttgac cgactcgccc ggcgcgaccg
     1621 agaccgtcgt cgagctggag gccccctcgc gcttgacgat cagctcgccg gggacgtatg
     1681 ccggggctgc gctcgccggg cccgcggcga cggcggccat cagggccagc gtggccagcg
     1741 tcgcgaagag gaaggaggct cggggcatcg tctacggtcc aatcaccagt gtatgaggac
     1801 gacaacaccg cggacgcagg caagttgtca cctggggcca atcggtattc gcgaggcaga
     1861 gcttgaggac ctgcgtcacg ggcgcgaccg gattcgtcgg cggccatctc acccgtgagc
     1921 tgatcgagcg cggccacgat gtgcgtgtca cctaccgcga cgccgcacgg ctggagcgac
     1981 tgcgcggctt cgaccccgag ccggtgcggg ccgacatcct cgaccgcgcc gcgatgcgcc
     2041 gggcggtacg cggctgcgac gtcgtcttcc acacggcggg gatcgtcgcc tcgaagcccg
     2101 tgtcgtacgt ccaggacatc aacgcgctcg cgccgcggat cgccgtcgag gcggccgctg
     2161 cggagggggt gccgcgcgtg gtcgtgacct cgagcgtcgc ggggatcggt ccggtcccgc
     2221 gcgggcaggt cgggcgcgag gaggacctct accgcggcgg cgggctcggg ctgacctatc
     2281 ccgacgccaa gcacgagggc gagatggagg cgttcgccgc cggcgcacgg ctcggtgtcg
     2341 aggtcgtcgt caccaacccg gcctacgtgc tcggcgtgcc gctcgaccgg cgccagccgg
     2401 gggagacgtc gacgcggatc atcggcaact acctgcgcgg gcgcctgccc gcggtggtcg
     2461 acggcgggac gacgatcgtc gacgtgcgcg acgtcgccga cggccacctg ctcgccgccg
     2521 agcgcggcaa gtcgggcgag cgctacgtgc tcggcggcca caacacgagc tggccggagc
     2581 tgatggcgaa ggtggcgcgg ctcgcgggcg tcgactaccc gctcgcggtc ttcccgcacg
     2641 gcgtcggagc gctggcgcgg cgcgccgagg cgctgcggct gccgctggcg atctccgccg
     2701 aggcgatggt gctgatggag cagaactggt gctactcgtc ggccaaggcg aagcgcgagc
     2761 tcggctaccg gccgcgttcc cacgattcga cggtccgcgc gacggtcgac tggtaccgcg
     2821 agctgatcga cgccggggtg ctcgacggcg gcagccgctc gccgctctcg ctcggcgcgg
     2881 ccgggctgcg gctggccgac cgggtcggag cgcttgcggt ggtcgacgcg ctcgggcgcc
     2941 ggacggggca ccggctggtg gcgcggtgag gggcgcgccc gcgcttgggg ccgtcacgcg
     3001 ggccgggcgc tgagccgcgg tggccgctcc cttcttcccg cgcgacgagt acttcatgcg
     3061 gctggcgctg cgcgaggccg agcgcgcgct cgagcacgag gacgtcccgg tcggctgcgc
     3121 gatcgtcctc gacggcgagg tgatcgccgc agcgcgcaac gagcgcgagc tgcgcgggga
     3181 cccgaccgcg cacgccgagg tgctcgcgct gcgcgaggcg tcgcggcgtg tcggccggtg
     3241 gcgcctcgac ggctgcgcgc tctacgtgac gctcgagccc tgcgcgatgt gcgcgggcgc
     3301 gatcgtcctc gcccgcctcc cgcgcgtcgt cttcggagct accgacccca agaccggcgc
     3361 agccgggtcg gtcctcgacg tgctcgccga gccgcgactc aaccatcgcc ctcaggtcgc
     3421 cggcggcctg ctcgccggcg agtccgccga cctcctgcgc gccttcttcg cctcccgccg
     3481 ctgacccgaa cgaccctcag cgagggcgcg aggccggccg gacctcggcg tcgagcaggc
     3541 cctcgcaggc gcgcgtgacg aggtcgagca cgtcctcgaa gccgtcgacc tcgccgtagt
     3601 aggggtccgg gacctcgagc tcgccggcgg cgaccgcctc ggggtcgtac tcgcgcagca
     3661 gcctgacgcg cgcgcgggcg tcctcgtcgg gcgcgagggc aagcaggtcg tcgtggttgg
     3721 cgcggtccat cgcgaggatc aggtcgtagg cgtcgaagtc ggcggcatcg acctggcggg
     3781 cctggcccgt gagcgcgatg ccgcgatcgc gcgcagccgc tgtcgctcgc tcgtccgggg
     3841 catggccgac gtgccagtca cccgtgccgg cgctgtcgat ctcgaccgcg tcgccgagac
     3901 cctcccgctc gagcaggtgg cgcatgaccc cctcggcggt cggcgagcgg cagatgttgc
     3961 cgaggcagac gaacagcagg cgcatcgcag gccgtcagac ccgggcggga tcctcgacgg
     4021 ggtcgaggac gccgagcagc tcgccgacgc ggcagaccag caaccgctcc tcctcgacct
     4081 cgatccagac gcccgccgac gccgggaaga cgacgtggtc gcccggcttg accggcatct
     4141 tcacgccgac cgactcccac cagtcgagcc cctggccgac cgccagcacg atcccgtgct
     4201 ggggcggcgg ctcgttggtg cccggcggca cgacgagtcc cgaccggcgc acccggtcag
     4261 ggtcgagctc cttgatcact acgcggtcga acagcggccg cagctcctgg cgcatggtcc
     4321 gagaatacgt gccggccggc aggtcgtatc gtcccgtcca tgaccaccac gacgacgccg
     4381 ggcgccgacg cctacagcct cccccaggag ttccgggact tccgcgacac gatccgcgac
     4441 ctcgtgcagg gggtggtcgc gccccgcgcc gccgagatcg acgcgaccgg cgagtacccg
     4501 tgggacgttc gcaaggcgtt cgccgaccac gacctgctcg ggctgccgtt ccccgaggag
     4561 ctcggcggca ccggcaccgg aacgctgatg ctgaacatcg cgatcgagga ggtcgccaag
     4621 gcgtgcgcgt cctcgtcgct gatcctgatg atccaggagc tcggcacgct gccgatccag
     4681 ctgcacggaa ccgacgagca aaagcggcag tggctgccgc gctgcgccgc cggcgagtgg
     4741 acgccggcct tctgcctgtc cgagcccgag gccggctccg accccggcgc gatgcgcacg
     4801 accgccgtcc gcgacggcga cgagtgggtg atcaacggca cgaagaactg gatcaccaac
     4861 gcgacgatcg ccgacttcta cgtcgtcttc gccgtgaccg accgcgagaa caagcgcttc
     4921 tcggcgttca tcgtcgagaa ggatcgcgag ggcttctcgg tcccgaagct cgagcacaag
     4981 ctcggcatca agggctcccc gaccggccag ccggtcttcg aggacgtccg cgtgccggcc
     5041 gaccacctga tcggcgacga gggccgcggc atggccgtcg cgctctcgac gctcgagcgc
     5101 acgcggctcg gtgcgggcgc ccaggcggtc ggcatcgccc agggcgccac ggactacgcc
     5161 gcgagctacg ccaaggagcg catctccttc ggcaagccga tcttcgacca ccaggcgatc
     5221 gccttcaagc tcgccgacat ggagacgaag accgccgccg cgcgcgagct gctctacaag
     5281 gcctgcgcga tggccgaccg caacgagcgc ggcctcggca agtactcctc gatggcgaag
     5341 ctgttcgcct ccgacgtcgc gatggaggtc acggtcgagg cggtccaggt gctcggcggc
     5401 tacggctacg tgaacgagta cccggtcgag cgcatgatgc gcgacgccaa gatcaccca
//
LOCUS       DATABV010000116        11023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1964468, whole genome shotgun sequence.
ACCESSION   DATABV010000116 DATABV010000000
VERSION     DATABV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11023
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964468"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VHF88_04835"
     CDS             complement(<1..270)
                     /locus_tag="VHF88_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2161130.1"
                     /translation="MADDPVFLITGASSGIGAATARHAAEAGYRLVLAARSEDKLAAL
                     AGELGGGDRALAVATDVTSWEDNEALVAAALDSFGRLDIAFANAGF"
     gene            449..934
                     /locus_tag="VHF88_04840"
     CDS             449..934
                     /locus_tag="VHF88_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161131.1"
                     /translation="MALLLAPSAQAAKCPAKYSRADFHRIAAKAYDGRQPTTRHERRT
                     LARVVRCQKRPRASRTLIRDHRGRWRRRHLRGLELRSIAPYSCGRHGRFAVPCSIVER
                     ESRYSWSAYNASSGALGPYQFLGWRVPWPVRTASDRLAHHRMAARLWSGGAGASHWGG
                     W"
     gene            complement(951..2069)
                     /locus_tag="VHF88_04845"
     CDS             complement(951..2069)
                     /locus_tag="VHF88_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2161132.1"
                     /translation="MGRRRWTALAALAVVALLLGVAAGVTWNYSSLVLVPDHDERFYE
                     EEVRASSPGRVVLERDEDTERPGIYGLEWEGGHAIAGRVVDSDADTVTRSLRRLRRLR
                     GHLPAGTKVELDQDVYSGDPRQARGLPFERVSIKGELGALPAWRIPGRGSTWAIFVHG
                     INGSPRAGLRIAPALRRAGLPSLLITYRNDPGAPPAPDGLHHLGMTEWRDVEAAARFA
                     VRRGARRLVVIGYSMGGAIVTQFAQRSPLARRLSALVLDAPALSWKPIFDFQAGERGL
                     PKLTSTALETAIDLRIPIDWDRLDALRHTAELDLPILLFHGLEDDVIPISISDGFANR
                     LPDSVTYHRVPHAGHVESWNVDPRLYERRLRDFLRKRL"
     gene            complement(2115..3722)
                     /locus_tag="VHF88_04850"
     CDS             complement(2115..3722)
                     /locus_tag="VHF88_04850"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX2161133.1"
                     /translation="MPSAPALPPAAAKPVKPIKVKPGKPAHAGKQGQPSAVAEPPSPD
                     PPVASATPTAPATSPAAPVSPAPAAPATTPVPVDNGRANDGRPSRRSTGSDRRGRHLN
                     RRTASGPLRATPDRLAGDASTRGRQPASPARRASDEGRRARERRPDRQAGGGSSPVVR
                     TVRDIIETVPAWMKLALAALGLLSLSLAGGYAVTALRARSLARQRTELLGEVGLLQRA
                     LLPAIPDELPGLRASVAYRPADGPAAGGDFYDVLRLTGDRVGFVIGDVSGHGREALER
                     TAFMRYTLRAYLEAGLAPRAALQVAGRVIGDGLGGDFATVLLAIHDPRDGSLTYACAG
                     HPAPIVVHGAPFDPVVAGSSPPIGIGERTGLRQTTVPLPPGTLACLYTDGLAEARTPD
                     GLLGRERLGQLVAEPGPGASATDLLERVAVEADAVPDDMATCLLTPTASVTSGGFRSE
                     QLELSADELSGPLPERFLDECGVPAAARADVLDEALRLSGGSGGAVLSVVFGSRRTVE
                     VLPCNVESIEVAALSARTAPRRLSAAL"
     gene            complement(4027..5592)
                     /locus_tag="VHF88_04855"
     CDS             complement(4027..5592)
                     /locus_tag="VHF88_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2161134.1"
                     /translation="MSEHERDDLTELLARRALTRDAARPDAVERRRAAGGRTARENVE
                     DLVDPGSFVEYGRFAIAAQRQRRELDDLIARTPADGLIGGTARVNGDLFGEHAACAVL
                     SYDYTVLAGTQGALGHRKKDRLFELIERMRLPTVFFAEGGGGRPGDTDYPVVSALDVR
                     AFALWARLSGVAPRIAVVAGRCFAGNAVIAGCSDLIVATENASLGMGGPAMIEGGGLG
                     KVEPDAVGPIEMQAPNGVIDVLVADEREATAVAKRLLGYFQGPTPPGREPDQRRLRDM
                     VPERQRRAYAVEPIVETLADEGSVAFLRERFGREIVTALGRIEGRPVGFVANDTRHTA
                     GAITSDAADKAARFMQLCDAFALPIVSLVDTPGMMVGPEAEATGLVRHGSRLLVTGAA
                     LRVPLVAVVLRRGYGLGAQAMMAGSTHEPLLTVAWPSAHLGPMGLEGAVRLALRKELD
                     AIPDEAEREQRVRDLTAAAEDNAKALNAATLFEIDDVIDPAETRALIASTLATAAAHP
                     GEPSAERRFVDTW"
     gene            5699..6100
                     /locus_tag="VHF88_04860"
     CDS             5699..6100
                     /locus_tag="VHF88_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161135.1"
                     /translation="MSTKPTPIDEELAAYVTAHAGGRDEVLLAVERETAELGSLANMQ
                     TSPEQAALLELLARSVGARRAIEIGTFTGYGAIRIARGLGDDGSLLCCELEQRWADVA
                     RRNVDRAGVGERVESVMLGMADGLTLVRRLG"
     gene            6147..7709
                     /locus_tag="VHF88_04865"
     CDS             6147..7709
                     /locus_tag="VHF88_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2161136.1"
                     /translation="MPSQGNRTLILAAMIFAVSMTFIDQTIVALAVPELQKDLGLSET
                     GTQWIVNAYLLSLSALFAFGGRLADIAGHRRMVIVGVVTFATASALCGATPTGDLGEA
                     WMIVFRVIQGAGAAIMFPAALAIVVAAYPVGERGKALAVFFGITGALTAVGPMAGGYL
                     TEWTWRSIFWINVPVAIIALILTMRAKPSDERTPAPIDWAGTALIAAGMGLAVLGLQQ
                     SSIWGWDSVATWGCIVAGLLLLAAFARRELRTEHPLIDVRIFQDRGFAVNNAVLFLLM
                     IVFVPLFFFTSLYAQISLGEDASQAGLYLLVFFGGFGVASQWSGRIYDAKGVRAAVVP
                     GCALAAVGFYLWAGSLTDFDLGDQWIYIVMAGAGVGFVLGPASTDALNRVARNRYGEA
                     TGINQTVRNFGASIGLAVLGTILILRNKVNIEASLGDLGVPKERADEVAAALSQSGSG
                     EQSGSFGEISGRAAQRMFEAVQHDFAQSTQTIFYVMAGVMVVAFLVAVVGLPKGRVEG
                     ANEAEVPTPAPS"
     gene            complement(7710..9260)
                     /locus_tag="VHF88_04870"
     CDS             complement(7710..9260)
                     /locus_tag="VHF88_04870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX2161137.1"
                     /translation="MDRIERVARETFGWTELRPGQREAIEAVLSGRDTLAVLATGAGK
                     SAIYQVAALLVDGPTVVVSPLLALQRDQVNALDEATDEGAAHVNAAVGARERDRALRD
                     LRRDRLEFLFLAPEQLANEEVLAQLRRAEPSLMVVDEAHCVSEWGHDFRPDYLRLGEV
                     AEALGRPTVLALTATAAPPVRDEIVERLGMRDPAVVVRGFDRPNLWLGVERFRDAQRK
                     HARLLEWVREAEPGGIVYTATRRDADRIAADLAEQGQRACAYHAGMGKKARIAVEREF
                     MEDRADVVVATVAFGMGIDKPNVRWVLHASVSESVDSYWQEAGRAGRDGDAARALLLY
                     RPEDLGLRRFFVSGTIGADHLRAVDDLLRGPAAKADADALAARLKLSPRRVRAILGRL
                     DDAGGVEAAVEREQRRRDFDRTRVEMIRAYAEHGSCRREFSVSYFGEPFQGPCGNCDN
                     CEAGRYAESEEVDTYPAGSRVRHDRWGEGTVQRTEDSRLVVLFDEGGYRMLELELVEA
                     GGLLEAVA"
     gene            complement(9296..9814)
                     /locus_tag="VHF88_04875"
     CDS             complement(9296..9814)
                     /locus_tag="VHF88_04875"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX2161138.1"
                     /translation="MAVPVKDSVRSGDPVQEPQPRERPVRGYATLTGLFLTLVASFSA
                     WIRASGRELPEKVSPGDLALVAIATHKASRLIAKERVTSAVRAPFTEVEPDPRAPRPK
                     FRERARGEGVRRAVGELVLCPYCLSVWIATAFAGGLVVAPRPTRFIAGILTTVFGSDV
                     LQIAYKKAEDSL"
     gene            9906..10697
                     /locus_tag="VHF88_04880"
     CDS             9906..10697
                     /locus_tag="VHF88_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2161139.1"
                     /translation="MNTLLKSNVTVSGQPTGQPMVFAHGFGCDQNMWRYVTPAFEDRY
                     RVVLFDHVGAGKSDLSAYDSRRYSSLDGYAEDVLEICAALELERVVFVGHSVSAMIGV
                     LAAIREPERFDKLVLIGPSPCYVDDDGYVGGFSRADVDELLDSLDSNYLGWSTAMAPV
                     IMGNPDRPELGAELTNSFCATDPEIASQFARVTFLSDNRSDLPRLAIPTLVLQCSEDP
                     IAPRPVGEFVRDQIPDSRFMQLSATGHCPNLSHPDETVAAIEAFL"
     gene            10694..>11023
                     /locus_tag="VHF88_04885"
     CDS             10694..>11023
                     /locus_tag="VHF88_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2161140.1"
                     /translation="MSDRAATSGDGRERDLPPQPSAEELYEDAPCGYLLALPDGTVIR
                     ANRTLLEWTGHSHEEIVGRRFADLLSAGGRIYHETHYAPLLAMQGAVREIALEMVRAD
                     GTRMPVLV"
BASE COUNT         1492 a         4002 c         3942 g         1587 t
ORIGIN      
        1 gaagccggca ttggcgaacg caatgtcgag gcgcccgaac gagtccaggg ccgccgccac
       61 cagtgcctcg ttgtcctccc agctcgtcac gtcggtcgcc accgcgagcg cacggtcgcc
      121 accgcccagc tcgcccgcta gcgccgcgag cttgtcctcg gaccgcgcag ccaggaccag
      181 ccggtagccg gcctccgccg catggcgggc tgtcgccgcg ccgatgcccg agctggcgcc
      241 ggtgatcagg aagacgggat cgtcggccat cacgccaact ctacgcagtg tgcgccccgt
      301 cacagttgcg gcgatcgcgg cggctaccgt cctaggacgt tctggcccga gtcgctgagt
      361 acggcgcggc tccctggcct gtccacgtcc ccgactcctc ggagctgagt cctcgatccg
      421 cccattgctc ttgtcatttc cccttgccat ggcgcttctg ctcgcaccct ccgcgcaggc
      481 cgccaagtgc ccggccaagt acagccgcgc cgacttccac cggatcgcgg cgaaggccta
      541 cgacggtcgc cagccgacca ctcgtcacga gcgccggacg ctcgcgcgcg tggtgcgctg
      601 ccagaagcgg ccacgcgcct cgcggacgtt gatccgcgat caccgcgggc gctggcgccg
      661 gcgccacctg cgcgggctcg agctgcgcag catcgccccg tacagctgcg gcaggcacgg
      721 ccgcttcgcg gtgccgtgct cgatcgtcga gcgcgagtcg cgctactcgt ggagcgccta
      781 caacgcctcg tccggggcgc tcgggcccta ccagttcctc ggctggcggg tgccgtggcc
      841 ggtgcgtacc gcgagcgatc gcctagccca ccaccgcatg gctgcccgtc tctggtccgg
      901 cggtgcgggc gcaagccact ggggcggctg gtgaccgttt gccgacgtgg tcagaggcgc
      961 ttcctgagga agtcgcgcag gcgccgctcg tagaggcgcg ggtcgacgtt ccaggactcg
     1021 acgtgtccgg cgtgcgggac gcggtggtag gtgacgctgt ccggtaggcg gttggcgaag
     1081 ccgtcgctga tcgatatcgg gatgacgtcg tcctcgagcc cgtggaagag gaggatcggc
     1141 aggtcgagct cggcggtgtg gcggagcgcg tcgaggcggt cccagtcgat cgggatgcgc
     1201 aggtcgatcg ccgtctcgag cgcggtcgag gtcagcttcg gaaggccgcg ctccccggcc
     1261 tgaaagtcga agatcggctt ccacgacagt gccggcgcgt cgaggaccag ggcgctgagg
     1321 cgacgcgcga gcggggagcg ctgggcgaac tgggtgacga tcgccccgcc catcgagtag
     1381 ccgatcacga cgaggcggcg agcgccacgg cggaccgcga agcgcgccgc cgcctcgacg
     1441 tcgcgccact cggtcatccc gaggtggtgc agcccgtccg gcgccggcgg cgcgcccggg
     1501 tcgttgcggt aggtgatcag cagcgacggc agcccggcgc ggcgcagcgc cggagcgatt
     1561 cgcagcccag ctcgcggcga gccgttgatg ccgtggacga agatcgccca ggtcgatccg
     1621 cggccgggga tccgccaggc cggcagcgcg ccgagctcgc ccttgatcga cacgcgctcg
     1681 aaggggaggc cacgcgcctg gcgcgggtcg ccgctgtaga cgtcctggtc gagctcgacc
     1741 ttcgtgccgg cgggcaggtg cccccgcagc cggcgcagcc ggcgcagcga gcgcgtgacg
     1801 gtgtcggcgt cggagtccac gacgcgaccg gcgatcgcgt ggccgccctc ccactcgagc
     1861 ccgtagatgc cgggacgctc cgtgtcctcg tcgcgctcga gcaccaccct tcccggtgag
     1921 ctggcgcgca cctcctcctc gtagaagcgc tcgtcgtggt ccggcaccag gaccagcgac
     1981 gagtagttcc aggtcacccc ggccgcgacg ccgagcagta gcgcgacgac tgcgagcgct
     2041 gcgagcgctg tccacctgcg gcgtcccact gcgcgagcct atctgcggcc gctgacagga
     2101 tccggcacag gcgcctagag cgctgcgctc agccggcgcg gcgccgtccg cgcggacagc
     2161 gccgcgacct cgatgctctc gacgttgcat ggcaggacct cgacggtccg acggctgccg
     2221 aagacgacgc tgaggacggc tccgccggaa ccgccggaga gtcggagcgc ctcgtcgagc
     2281 acgtccgcgc gcgccgcggc cggcacgccg cactcgtcga ggaagcgttc gggcagcgga
     2341 cccgagagct cgtcggcgga aagctcgagc tgctcgctgc gaaagccgcc tgacgtgacg
     2401 ctggccgtcg gcgtcagcag acaggtcgcc atgtcgtcgg ggacggcgtc ggcttcgacg
     2461 gcgacgcgct cgagcagatc ggttgccgag gcgccgggtc cgggctccgc taccagctgg
     2521 cccaaccgct cgcggccgag cagaccgtca ggcgtgcgag cctcggccag accgtccgta
     2581 tagaggcagg cgagtgtccc gggcgggagc ggcacggtgg tctgacgcag tcccgtgcgc
     2641 tcgccgatgc cgatcggcgg cgacgagccg gcgaccaccg ggtcgaacgg ggcgccgtgg
     2701 acgacgatcg gcgccgggtg cccggcgcac gcgtaggtga gcgatccgtc gcgtgggtcg
     2761 tggatcgcga gcagtaccgt cgcgaagtcg ccgccgaggc cgtcgccgat cacgcgaccg
     2821 gctacctgga gcgcggctcg cggcgcaagg ccggcctcga ggtacgcgcg caacgtgtag
     2881 cgcatgaagg ccgtgcgctc gagcgcctca cggccgtggc cggacacgtc gccgatcacg
     2941 aagccgacgc ggtcgccggt caggcgcagc acgtcgtaga agtcgccgcc cgcggcaggc
     3001 ccgtcggccg gcctgtaggc gacggaggcg cgcaagccgg ggagctcgtc cgggatcgcg
     3061 ggaagcagcg cgcgctgcag cagtccgacc tcgcccagca gctcagtgcg ctggcgggcc
     3121 agcgagcgcg cgcgcagggc cgtgacggcg taaccgccgg caagcgatag cgacagcagg
     3181 ccgagagcgg cgagcgcgag cttcatccac gccgggaccg tctcgatgat gtcgcgcacg
     3241 gtgcggacga ccggtgagct gccgccgccg gcctgacggt ccgggcggcg ctcgcgtgcg
     3301 cggcggccct cgtcggacgc gcgtcgtgcg ggtgacgctg gctgcctgcc gcgggtcgac
     3361 gcgtcgccgg caaggcgatc cggcgtcgcc cgcagcgggc cgctggccgt ccgtcggttg
     3421 aggtgccgtc cgcgccggtc gctcccagtc gatcgcctgc tcggtcggcc gtcgttcgcc
     3481 cgtccgttgt cgaccggcac cggggtcgtc gccggtgccg cgggagcggg agagaccggc
     3541 gcggctggcg aggtcgccgg ggcggttggc gtcgctgagg cgaccggcgg gtcgggagag
     3601 gggggctccg ccaccgccga tggctgcccc tgcttgcccg catgcgcagg tttgcccggc
     3661 ttcaccttga tcggcttgac cggcttcgcg gcggccggcg ggagggccgg cgcggacggc
     3721 accggcgcac tcgtaccctg gatcgacccc tgtccgggct ggccggtcgg gagccgctcc
     3781 agcaccttct tggcctgtcc tggcggcagc gagcccatgg cgccgtcctg tttcgcagcg
     3841 gccgtcgccg gagcgattgc cgcaaccatc agggcgcata ccgtcacggc gcttcgcgag
     3901 ttgaagccca tccctccggc ttcgacagcc gccgcgcacc cgctgagcct ccgcgacgcc
     3961 cgccgaacga acgtccaccg tcacgcggtc gacgcgtccg gcaggagaga ctcgctgttg
     4021 ctcccgctac caggtgtcga cgaagcggcg ctcggcgctc ggctcgcccg ggtgcgccgc
     4081 ggctgtggcc aatgtcgagg cgatcagggc gcgtgtctcg gccgggtcga tcacgtcgtc
     4141 gatctcgaac agtgtcgccg cgttgagcgc cttcgcattg tcctcggcgg cagcggtcag
     4201 gtcgcgcacc cgctgctcgc gctcggcctc gtcggggatc gcgtcgagct ccttgcgcag
     4261 ggcgagcctg actgcaccct cgaggcccat cgggccgagg tgcgccgacg gccaggcgac
     4321 tgtcagcagc ggctcatgcg tgctgccggc catcatcgct tgcgcgccga gcccatagcc
     4381 gcggcgcagg accacggcga cgagcggcac gcgcagtgcg gcgccggtga ccagcagccg
     4441 cgagccatgg cgcacgaggc cggtcgcctc cgcttcgggg ccaaccatca tccccggcgt
     4501 gtcgacgagc gagacgatcg gcagggcgaa ggcatcgcag agctgcatga agcgcgcggc
     4561 cttgtccgcc gcgtcgctgg tgatcgcgcc cgccgtgtga cgcgtgtcgt tagcgacgaa
     4621 gccgaccggc cgcccctcga tccgtccgag cgccgtgacg atctcgcggc cgaagcgctc
     4681 gcgcaggaag gcaaccgatc cctcgtcggc gagcgtctcg acgatcggct cgacggcgta
     4741 ggcgcgccgc tggcgctcgg gcaccatgtc gcgcagccgc ctctggtccg gctcgcggcc
     4801 cggcggcgtt ggcccctgga agtagccgag cagccgcttg gcgaccgccg tcgcctcccg
     4861 ctcgtcggcg accaggacgt cgatgacgcc gttgggtgcc tgcatctcga tcgggccgac
     4921 ggcgtcgggc tcgaccttgc cgagccctcc gccctcgatc atcgcggggc cgcccatgcc
     4981 gagcgacgcg ttctcggtgg cgacgataag gtcggagcag ccggcgatca cggcgttgcc
     5041 ggcgaaacag cggcccgcga cgacggcgat ccgcggggcc acgccggaga gacgtgccca
     5101 tagcgcgaag gcgcgcacgt cgagcgcgga tacgacgggg tagtccgtgt ctccgggccg
     5161 tcctccgcca ccctcggcga agaagaccgt cggcaaccgc atgcgctcga tcagctcgaa
     5221 caggcggtcc ttctttcggt gaccaagcgc gccctgggtt cccgcgagca cggtgtagtc
     5281 gtaggagagg accgcgcacg ctgcgtgctc gccgaacaga tcgccgttca ctcgagccgt
     5341 cccgccgatc agcccgtcag cgggcgttcg cgcgatcagg tcatcgagct cgcggcgctg
     5401 gcgctgagcc gcgatcgcga agcgcccgta ctccacgaac gaccccgggt cgacgaggtc
     5461 ctcgacgttc tcgcgcgccg tccggccacc ggcggcacgg cgccgctcga ccgcgtcggg
     5521 ccgcgccgcg tctcgtgtca gcgcacggcg cgccagcagc tcggtcagat cgtcgcgctc
     5581 gtgctcgctc attccgcgcc tcctcgtcac cggctcccca ttgcggcccg atcatggcgg
     5641 acctcaggga ggagcggagg atcgtcccgg accgcgggcg cccgctagcg tcacgttcgt
     5701 gtccacgaag cccacgccga tcgacgagga gcttgcggcc tatgtgaccg cgcacgcggg
     5761 cggacgcgac gaggtcctgc tggcggtcga gcgcgagacc gccgagctcg gttcgctcgc
     5821 gaacatgcag acgagccccg agcaggcggc gctgctcgag ctgctagcca ggtcggtcgg
     5881 ggcgcggcgc gcgatcgaga tcgggacctt caccggctac ggagcgatcc ggatcgcgcg
     5941 cgggctcggc gacgacggct cgctgctgtg ctgcgagctg gagcagcgct gggccgacgt
     6001 cgcgcgccgc aacgtcgacc gcgcgggcgt cggcgagcgc gtcgagtccg tgatgctcgg
     6061 catggccgac ggcctcacgc tcgtccgccg cttgggctag ggcctccgca ggaccgagcc
     6121 gggtggtggc gaatggtctt ccgacgatgc ctagccaggg caacaggacg ctgatcctcg
     6181 cggcgatgat cttcgcggtg tcgatgacct tcatcgatca gacgatcgtc gcgctcgccg
     6241 tcccggagct tcagaaggac ctcgggctgt cggagacggg tacgcagtgg atcgtcaacg
     6301 cctacctgct gtcgctctcg gcgctgttcg cgttcggcgg gcggctcgcc gatatcgccg
     6361 gccaccggcg gatggtgatc gtcggggtgg tcacgttcgc gacggcgtcc gcactctgcg
     6421 gggcgacccc taccggcgat ctcggcgagg cctggatgat cgtcttccgc gtgatccagg
     6481 gcgccggcgc ggcgatcatg ttccccgccg cgctggcgat cgtcgtcgcg gcctacccgg
     6541 tcggcgagcg cggcaaggcg ctcgcggtct tcttcggtat caccggcgcg ctcaccgccg
     6601 taggcccgat ggccggcggc tacctaaccg agtggacctg gcgctcgatc ttctggatca
     6661 acgtgccggt ggcgatcatc gcgctgatcc tgaccatgcg cgcgaagccg tccgacgagc
     6721 gtaccccggc gccgatcgac tgggccggca ccgcgctgat cgcggccggc atgggcctag
     6781 cggtgcttgg gctgcagcag tcgagcatct ggggctggga cagcgtcgcg acatggggct
     6841 gcatcgtcgc cggactgctg ctgctcgccg ccttcgcccg ccgcgagctg cgcaccgagc
     6901 acccgctgat cgacgtgcgg atcttccagg accgcggctt cgcggtcaac aacgccgtcc
     6961 tgttcctgct gatgatcgtc ttcgtcccgc tgttcttctt caccagcctc tacgcgcaga
     7021 tctcgcttgg cgaggatgca tcccaggccg gcctctacct gctcgtcttc ttcggcggct
     7081 tcggtgtcgc ttcgcagtgg agcgggcgga tctacgacgc caagggcgtg cgcgccgccg
     7141 tcgtgcccgg ctgcgccttg gccgccgtcg gcttctacct ctgggccggc agcctgaccg
     7201 acttcgacct cggcgaccag tggatctaca tcgtgatggc cggtgccggg gtcggcttcg
     7261 tgctcggtcc ggccagcacc gacgcgctca accgcgtcgc gcgcaaccgt tacggcgagg
     7321 cgaccggcat caaccagacc gttcgcaact tcggcgcgag catcggcctg gccgtgctcg
     7381 gcacgatcct gatcctccgc aacaaggtga acatcgaggc ctcgctcggc gacctcggcg
     7441 tgccgaagga gcgggcggac gaggtcgccg cggcgctatc gcagtccggc agcggcgagc
     7501 agtcgggctc cttcggtgag atctccggcc gcgcggccca gcgcatgttc gaggccgtcc
     7561 agcacgactt cgcccagtcg acgcagacga tcttctacgt catggccggc gtcatggtcg
     7621 tggccttcct cgtcgccgtc gtcggcctgc cgaagggccg tgtcgagggc gccaacgagg
     7681 ctgaggtacc gacgcccgct ccctcctagc tacgccaccg cctccagcag cccgcccgcc
     7741 tcgacgagct ccagctcgag catccggtag ccgccctcat cgaacagcac gaccaggcgg
     7801 ctgtcctcgg tccgctggac ggtaccctcg ccccagcggt cgtgacggac gcgcgacccg
     7861 gcggggtacg tgtcgacttc ctcgctctcg gcgtagcgac cggcctcgca gttgtcgcag
     7921 ttcccgcacg gcccttggaa gggctcgccg aagtaggaga ccgagaactc gcgccggcac
     7981 gatccgtgct cagcgtaggc gcggatcatc tcgacgcgcg tgcggtcgaa gtcgcgtcga
     8041 cgctgctcgc gctcgacggc tgcctcgacc ccgccggcgt cgtcgaggcg cccgaggatc
     8101 gcccgcacgc gccgcggcga gagcttcagg cgcgcggcca gcgcgtccgc gtcggccttc
     8161 gccgctgggc cgcgcagcag gtcgtcgacg gcgcgcaggt ggtccgcgcc gatcgtgccg
     8221 ctgacgaaga accggcgcag cccaaggtcc tcggggcggt agagcagcag cgcccgcgcg
     8281 gcgtcgccgt cgcggccggc gcgcccggcc tcctgccagt aggagtcgac cgactcactg
     8341 acgctcgcgt gcaggaccca gcgcacgttc ggcttgtcga tccccatacc gaaggcaacc
     8401 gtcgcgacga cgacatcggc gcgatcctcc atgaactccc gctcgacggc gatccgcgcc
     8461 ttcttgccca tcccggcgtg gtaggcgcac gcacgctgcc cctgctcggc gaggtcggcg
     8521 gcgatccggt cggcgtcgcg ccgggtcgcg gtgtagacga tcccgccggg ctcggcctcg
     8581 cggacccact cgagcaggcg ggcgtgcttg cgctgggcgt cgcggaagcg ctcgacgccg
     8641 agccagaggt tcggccggtc gaagccgcgc accacgacgg cggggtcgcg catgccaagc
     8701 cgctcgacga tctcgtcgcg gaccggggga gcggccgtcg ccgtcagcgc caggaccgtc
     8761 ggccgcccga gcgcctcggc cacctcgccg agacgcaggt agtcggggcg gaagtcgtgc
     8821 ccccactcgc tgacgcagtg cgcctcgtcg acgaccatca gcgacggctc ggcgcggcgc
     8881 agctgcgcca ggacctcctc gttggcgagc tgctcgggag cgaggaagag gaactcgagc
     8941 cggtcccggc gcagatcgcg cagcgcgcgg tcgcgctcac gggcgccgac cgccgcgttg
     9001 acgtgcgcgg cgccctcgtc ggtcgcctcg tcgagcgcat tgacttgatc gcgctggagt
     9061 gcgagcagcg gcgagacgac gacggtcggg ccatcgacca gcagcgccgc cacctggtag
     9121 atcgccgact tccccgcgcc cgtcgccaga acggcaagcg tgtcgcgacc gctgagcacg
     9181 gcctcgatcg cctctcgctg tcccggacgc agctccgtcc acccgaacgt ctcgcgcgcg
     9241 acgcgctcga tgcggtccat ctacgaaggt tgtgcgtccg gcgtgcgacc gctcgctaga
     9301 gcgagtcctc ggcctttttg taggcgatct ggaggacgtc ggagccgaag acggtggtca
     9361 ggatcccggc gatgaagcgc gtcggacgcg gcgccacgac gaggccgccg gcgaacgcgg
     9421 tggcgatcca gacgctgaga cagtaggggc agagcacgag ctcgccgacc gcgcgccgga
     9481 cgccctcccc gcgggcccgc tcgcggaact tgggcctggg ggcgcgcggg tccggctcga
     9541 cctcggtgaa cggcgcacgg accgcgctcg tcacgcgctc cttggcgatc aggcgcgacg
     9601 ccttgtgggt ggcgatcgcg accagcgcga ggtcacccgg cgacaccttc tcgggcagct
     9661 cacggcccga cgccctgatc caggctgaga agctcgcgac gagcgtcagg aagaggccgg
     9721 tcagcgtcgc gtagccgcgc actgggcgct cgcgcggctg cggctcctgg actgggtctc
     9781 cggaccggac ggagtctttt acgggcacgg ccatgaccgc cctactaccc ggatcgggca
     9841 agcccatgca taggcagata gtcagcgtct tctcgccacg acccgacgcg gttaatctcg
     9901 ccttggtgaa cacgctgctc aagagcaacg tgaccgtcag cggccagccg accgggcagc
     9961 cgatggtctt cgcgcacggc ttcggctgcg accagaacat gtggcggtac gtgacgccgg
    10021 ccttcgagga ccgctaccgc gtcgtgctgt tcgaccatgt cggcgcgggg aagtcggatc
    10081 tctccgccta cgactcgcgc cgctactcgt cgctcgacgg ctacgccgag gacgtgctcg
    10141 agatctgcgc cgcgctcgag ctcgagcggg tcgtctttgt cgggcactcg gtcagcgcga
    10201 tgatcggtgt gctggctgcg attcgcgagc cggagcggtt cgacaaactc gtgctgatcg
    10261 ggccgtcgcc gtgctacgtc gacgacgacg gctacgtcgg cggcttcagc cgcgccgacg
    10321 tcgacgagct gctcgactcg ctcgacagca actacctcgg ctggtcgacc gcgatggcgc
    10381 cggtgatcat gggcaacccc gaccggccgg agctcggagc cgagctgacc aacagcttct
    10441 gtgcgaccga cccggagatc gcaagccagt tcgcgcgcgt gaccttcctc tccgacaacc
    10501 gctcggacct accgcggctc gcgatcccga cgctcgtcct ccagtgctcc gaggacccga
    10561 tcgccccgcg gccggtcggc gagttcgtcc gtgaccagat ccccgacagc cgcttcatgc
    10621 agctcagcgc gaccggtcac tgccccaacc tgagccaccc ggacgagacg gtcgcggcga
    10681 tcgaggcgtt cctctgagcg accgggccgc cacctcgggc gacggtcgcg agcgcgatct
    10741 accgccccag ccgagcgcgg aggagctcta cgaggatgcc ccttgcggct acctgttggc
    10801 gctgcccgac gggaccgtga tcagggcgaa ccggacgctg ctcgagtgga ccggccacag
    10861 ccacgaggag atcgtcggcc gccgcttcgc cgatctcctc agcgccggcg gacggatcta
    10921 ccacgagacc cactacgcac cgctcttggc gatgcagggg gccgttcgcg agatcgcgct
    10981 cgagatggtg cgggcggacg gcactcgcat gccggtgctg gtc
//
LOCUS       DATABV010000117        18410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1804903, whole genome shotgun sequence.
ACCESSION   DATABV010000117 DATABV010000000
VERSION     DATABV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804903"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..541)
                     /gene="atpH"
                     /locus_tag="VHF88_04890"
     CDS             complement(8..541)
                     /gene="atpH"
                     /locus_tag="VHF88_04890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX2161141.1"
                     /translation="MEEIAEVYSRALFEVAQQQGVLDRVHEELGQFADVLSESRDLQV
                     FFFSPYFSSQEKKDGIGALVEDADENFVRFLELLAERHRMPAIFRVRAELDRLWRREN
                     KLLPVSVTSAVELDSQLVEGIGRQIEQQTGERVELTSRVDPDVLGGLVLQVGNMVLDA
                     SVRNRLERLRKQVAKAA"
     gene            complement(545..1135)
                     /gene="atpF"
                     /locus_tag="VHF88_04895"
     CDS             complement(545..1135)
                     /gene="atpF"
                     /locus_tag="VHF88_04895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX2161142.1"
                     /translation="MEAFTEIATLVLAATEAGAEDEGGSFLVSPDVGLMLWTLIAFGI
                     SMAILAKFAFPVIGEALDKRRRAIDESIDAAARTKEEADQLLEEYRARLREAREQAEE
                     IVARSRKAADSHMEDVKGAAKEYREELLATTRKDIEAETRRALDEIRKEVADLTVIAT
                     EKVTRKSLDDADHRRLIEEALSEVDFSALAGSEGRS"
     gene            complement(1146..1352)
                     /gene="atpE"
                     /locus_tag="VHF88_04900"
     CDS             complement(1146..1352)
                     /gene="atpE"
                     /locus_tag="VHF88_04900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX2161143.1"
                     /translation="MALGVGAGLGSIGAGIGIGIIFGKEIESVARQPEMAAELQSIRW
                     LGFALTEAVAFYTFIFGLIAFFLA"
     gene            complement(1428..2327)
                     /gene="atpB"
                     /locus_tag="VHF88_04905"
     CDS             complement(1428..2327)
                     /gene="atpB"
                     /locus_tag="VHF88_04905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX2161144.1"
                     /translation="MRTRTKVFIGLGLWLLLAVVFGVLFGSEGRNEAFQPQEEFRLQP
                     WIELNVLGIDMSINKAVMYLFLAAAATTATMVFIARRMQARPNRVQTAIEVAYDLTRN
                     NITGQNMGRPMATKWFSFVATLFFFIWFSNMLGYLPLPTNTEHPVNIFGAEVPALAIY
                     AATANLSIPLALTLVVWIAYHIEGIRVKGFVGFMKSFVPPGMSGVGAIPLFVIEVISD
                     IVRIISLSVRLFANILAGHLLILFMAGGLVVLLGISALGVITLGMAIPFFLFEIGLVA
                     TLQAFIFAILTSIYLGGATADSH"
     gene            complement(2324..2734)
                     /locus_tag="VHF88_04910"
     CDS             complement(2324..2734)
                     /locus_tag="VHF88_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161145.1"
                     /translation="MTAHAVVTPEGSGEAGFLLTLLRGLDLLVLALALPLFLIAGLPL
                     LGWAAVAVAWIAQRWIIALAKRRALASGDRARVMRVVGLSMLIRLMLVTASVAAVGLA
                     DRDAGLPAAILAAVLFSCALVLQLAAGPVAGARR"
     gene            complement(2807..4456)
                     /locus_tag="VHF88_04915"
     CDS             complement(2807..4456)
                     /locus_tag="VHF88_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2161146.1"
                     /translation="MDPPLRERLGWKTSNDPAANVPGGRAAMARTAFWFALAGSALAL
                     LVLAPPQGPDIDVTGLVVVAVGSATLAAIALIGFDRIPLWCFHAGTVLATLLATVAIY
                     SFGDQAPYGPLPYLWIAVYAFYFFPLPAALVHTAVLGALFGVELGLRDLDWTPIDSWV
                     ATIVTLSTAGLVVGVIRDRLTGMISRLTDAARTDPLTGLLNRRGFEEAFDVELERARR
                     ADRGLSVIVGDLDRFKELNDRFGHAAGDDVLCRVGETIRAMKRSWDVAARIGGEEFAI
                     VAPDTDEHGAYVLAERLRVEIEETFEPPGAGQLTASFGIVSFPIHGQTGEALLQAADQ
                     ALYAAKRLGRNRAVISSAEVPGILARAPHGRDEVHVELVTLLGLAEALDVRDSGSATH
                     SQRVGRYAELIARELGLPPDAVERLRIAGILHDVGRVGVPDELLRKRGPLTADEWTWI
                     RSHPAIGARMLDTTDFDDIGEWILAHHERPDGTGYPAGRTAGEVPLEASILAVADAYE
                     AMTAARPYRPALDAATASEELRRGAGRQFDERVVEAILRVV"
     gene            4620..5039
                     /locus_tag="VHF88_04920"
     CDS             4620..5039
                     /locus_tag="VHF88_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX2161147.1"
                     /translation="MWVSRDLVLEPRPRGFHLITSEVVGALPELRQLAAGLLQAQILH
                     TSASLTLNENASPDVRADFESWFTRAVPEDAPYWRHTLEGPDDMPAHIKASLLGPSVT
                     VPIRHGRPLLGTWQGIYLCEHRDRGGPRSLALTLTGE"
     gene            5090..6571
                     /locus_tag="VHF88_04925"
     CDS             5090..6571
                     /locus_tag="VHF88_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161148.1"
                     /translation="MRWDALSIEDEATRQLPGYREPATVRTFDAPEAMDIRFYEVRAK
                     SALNRVPEASRMPFRWTVNPYRGCTHACVYCLDGGTPILLLDGTTRPLSELRPGDRIV
                     GTELVGARRRYAITEVLAHWQTLKPAYRVALSDGTALVASADHRFLTRAGWRQVTGNE
                     PGGPRRRLSASDQLLGPGLAAGQTATAGEPVESSRGLWVDSIEPLGEERVLFDITTGT
                     GDFVANGVVSHNCFARPTHKFLDLSAGRDFEREIVVKVNAPEALRAQLSRPSWKREHV
                     ALGTNTDPYQWVEGRYRLMPGIWEALRDSGTPCSVLTKSPLLLRDMALMKELVEQVGF
                     TASLSVPTLDERAWRATEPHTPHPRARLEAVGELNRAGVPTGILIAPLMPGVNDSPEQ
                     VERILELATEAGATSVGGLHLHLRGEVRGIFMDWLRSERPDLVPRYEELYARGAYAPR
                     AERERVAALVGRRGPKWPRRGTLRPRREPPGQDDDGFEQPSLF"
     gene            complement(6586..7794)
                     /locus_tag="VHF88_04930"
     CDS             complement(6586..7794)
                     /locus_tag="VHF88_04930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX2161149.1"
                     /translation="MNDLDALWAFLVAAAVALLLTPLTARLATRVGAVALPRERDLHD
                     APMPRLGGLAILAGVLLAGALFLPGDQATRGILAGAAAITLVGAVDDAKPGGLNPVLK
                     LLGQLAAALIPVACDVRVENITLPFVDPLDIGDWGYPLTLLGIVAVMNVVNFTDGVDG
                     LAAGVCTIAAATFAVIALSLDRVDPAILAAATAGAAVGFLRHNFHPASVFMGDSGSNL
                     LGLLLACVAIQGVLKTAAVVALFFPLVILAVPAADATFVVAKRIKYRRPVYSADTWHF
                     HHRFANIGFSQRRTVLYLYAWTLSLAALALALRFVPYSDDGGSFNLGWTVVMAAFGLI
                     ALAASLYLVLVLEILKFRRFRERQLRREGPSLSEAEIEQEIEREVETGEFDAVSPPST
                     GPAPRGVRGA"
     gene            complement(7821..8522)
                     /locus_tag="VHF88_04935"
     CDS             complement(7821..8522)
                     /locus_tag="VHF88_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX2161150.1"
                     /translation="MAETAPRLDLEQAKRDIGQALRERPRWRGVTHQWAFFISLALGV
                     ALVVTAPPGRGTLAAAIYAACVTLLFGASALYHRVTWQTDSARRWMRRLDHSAIFLLI
                     AGTYTPFALLVLHGSLADVVLAVMWSGAVGGILLKLLWIDAPKRLAAVIYVALGFVAV
                     AVTPELISQVGVVASILVALGGVLYTLGAVTYALRRPDPVPAVFGYHEVFHALVIVAA
                     ALQYAVIAFWVLPQG"
     gene            complement(8620..9909)
                     /gene="glyA"
                     /locus_tag="VHF88_04940"
     CDS             complement(8620..9909)
                     /gene="glyA"
                     /locus_tag="VHF88_04940"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX2161151.1"
                     /translation="MTDLSPDYFSKPVSEVDPEIAQVLGDELRRQETTLEMIASENFV
                     PQAVLDCQGSVLTNKYAEGYPGKRYYGGCEYVDVAERLAIDRAKELFGAEHVNVQPHA
                     GAQANAAAFHALLQAGDRILGMKLDHGGHLTHGMKINFSGRLYDIVAYGVSDKDSRVD
                     MDVLERLAQEFRPKLILAGWSAYPRQLDFARFRDIADSVGAYLMVDMAHFAGLVAAGE
                     HPSPVPHADVVTTTVHKTLGGPRSGMILARQEHAKKIDSAVFPGQQGGPLMHVVAAKA
                     VAMKVAQSELFRERQQRTRAGAAAIAAELTAAGVEVLTGGTDVHLVLVDLRDSELDGQ
                     QGEDRLHAIGITVNRNSVPNDPRPPAVSSGLRIGTPALATRGFQLEDFREVGKVIATA
                     LTADFTESVRADLAERTRALADRYPLYPELGKAVATV"
     gene            complement(9999..10664)
                     /locus_tag="VHF88_04945"
     CDS             complement(9999..10664)
                     /locus_tag="VHF88_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX2161152.1"
                     /translation="MSGSHAARQAGAAGPLGEGDVAAFERCIAGGGIAVFPADTVYGL
                     AASPDSEPAVRRLQELKGREASQPSAVMFFALRPALAALPELGARTRTAFERLLPGPL
                     TLIVANPRGRFPLACGPDTSRLGVRVPALDGPLEPLTAVAVPVLQSSANLHGGADPRR
                     LADVPEEIRAGVDLALDGGDLPGVASTVVDLSQYEHDGRFDVLREGALPAGDLARLLR
                     DTG"
     gene            complement(10661..11470)
                     /gene="prmC"
                     /locus_tag="VHF88_04950"
     CDS             complement(10661..11470)
                     /gene="prmC"
                     /locus_tag="VHF88_04950"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX2161153.1"
                     /translation="MPAASMTARDAVDAAVDALAAAGCEMPRLDAEVLIADALGVDRA
                     ALISDSELAVPPASAREIGERIRRRVAREPVAYIVGRKGFRRIELAVDRRVLIPRPET
                     ELLVEVALELPSGAAVHDVGTGSGALALALLDERPDLRVTASDVSPSAVEVARANAAR
                     LGLPLEPFVADGLPAGEYDLVVANLPYVTDGEWTRLAPEIMQYEPRSALTAGADGLDV
                     IRTLVARAPRGTRLALEHGCEQGSEVRALLAGAETRSDLSGRERVTVGTVT"
     gene            complement(11470..12525)
                     /gene="prfA"
                     /locus_tag="VHF88_04955"
     CDS             complement(11470..12525)
                     /gene="prfA"
                     /locus_tag="VHF88_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX2161154.1"
                     /translation="MIQKLVEQIESRFAELSEQMSDPEVIGDRERYAEVGRAYSALEP
                     AAELAREWRTAASDAAGAREMLSEDGEDLEIRAMLETAEERLAALDEEIRLAMVEPDP
                     NDDKNVIVEVRGGTGGEEAGLFAGDLYRMLTRYAERLGFNTETMNVADGDYTFAIKGQ
                     GAYSVFKHEGGTHRVQRVPETESQGRIHTSTATVAVLPEVEDVEVEIDQNDLQIDVYR
                     SSGPGGQSVNTTDSAVRITHKPTGVVVSMQDEKSQLQNRERAMRVLRARLYERELAAQ
                     QAELAESRRSQVGTGERSEKIRTYNFPQDRVTDHRVKLTKGEVQRILAGELDDFTAAL
                     EAEEKRQRLEASSLVES"
     gene            complement(12546..13547)
                     /locus_tag="VHF88_04960"
     CDS             complement(12546..13547)
                     /locus_tag="VHF88_04960"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX2161155.1"
                     /translation="MSGEQQLQAQRDLPIGGQAVLEGVMMRGASTWAVAIRKPTPEQL
                     ETGGLNATEGAKGEIEVVTEPLVSYAKTHRWARLPVLRGMVALYESLKIGFRALGISA
                     NAQLAPDDRGETQEIGGATWAGTIAFALLFAIGLFFLLPAFTTNLFKDSIPNSVVFVV
                     IEKLVRIGVFLAYLWLVSRMRDLQRVFQYHGAEHKTISCYEAGLDLTPENAQRFSRLH
                     PRCGTSFLLIVMIVAIFVFAPLGKLDWQWLLISRVVGIPIVAGLAFEVIKWFGRNRAK
                     RWAQVLMWPGLQLQRLTTREPELSQLAVAIAALEAVLQFEDPADVSEAERVVMEVGA"
     gene            complement(13588..14352)
                     /locus_tag="VHF88_04965"
     CDS             complement(13588..14352)
                     /locus_tag="VHF88_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX2161156.1"
                     /translation="MSSRVSIVLLAGLAAVVALACATASVSGAPAPAADGSVELLECE
                     RGKRVADRNALFRGEMSRLPDAASMRMRFELRERLGRNAWRAVEAPGLGVWRHARPDV
                     ARFAYRQRIAALARGTAYRVVVEFQWHDAAGMLIERQAARSPVCRQAGPLPNLRVAGL
                     ETLAGPTAETVRYVATVVNRGAAPARRLEIALRVDGAEVDTRGVRRVAAHARRDVAFV
                     GPACARDVTVEVDPGDALRERNERDNARSFACAAMD"
     gene            complement(14403..14618)
                     /gene="rpmE"
                     /locus_tag="VHF88_04970"
     CDS             complement(14403..14618)
                     /gene="rpmE"
                     /locus_tag="VHF88_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX2161157.1"
                     /translation="MKTDLHPNYVEAHVRCTCGNEFTTRSTKPDLHVEVCSNCHPFYT
                     GKQKLIDTGGRVERFRRRQARGRAGAA"
     gene            complement(14713..15798)
                     /locus_tag="VHF88_04975"
     CDS             complement(14713..15798)
                     /locus_tag="VHF88_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX2161158.1"
                     /translation="MAPSRRHQSHAPRRASVVGGGSFGTAIALLLERAGVRTTLLCRT
                     GEQADALTEARENRRYLSGVELPAALKIRAFGAHDDQLRRPDLVFLAVPSKSLGEAIA
                     EIERLGVAPHAGMVSLAKGLVPPEGTPPTAALERAFGAERTACLGGPAHAREMVETGA
                     GLVCASRSEALALRVSGLFQRAGVVCEASDDPVGVELAGVAKNAAAVAVGATQSQGLN
                     AAGMAAADIFLEVLALAELWGGSARTFVGRSGTGDLVATALAPTSRNRSAGELLAAGT
                     PAAEIPARLGQAVEALETVRTLARAIERAGLEAPVTSALARLIDGDLPLDDWIALVRA
                     KQPPHARFARPRTWWARMRDWLGGRKP"
     gene            complement(15818..17557)
                     /locus_tag="VHF88_04980"
     CDS             complement(15818..17557)
                     /locus_tag="VHF88_04980"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HEX2161159.1"
                     /translation="MSSANGNGKVAPAAFPPPVKSPDEFIKRGLDPEDELIEIGVAIV
                     GGGTAGLACANRLLQLLADDPETMESLGEVPVAVIEKAKTRGGHNLSGAVVRPEPLQR
                     LFPDMTREDWRKEGFAFGEVDKEAVYMTPTSKRALRIPTPPPFKNHGNEVISVSALAR
                     YQQRIAEEAGAYLLTETAATQLIVEDGKVVGVRSGDKGRGKDGEPLGNFEPGTDIKAK
                     ATVLAEGCWGHLTGPAIREFDLGEGREPQTWELGVKEVWKVPKPLDRLIHTVGPWPVS
                     TSAKYGQTGGTWLYPMKDEKTGDDLVSIGFVIDLEYADATTSAHDLLQTFKMHPLVRK
                     ILEGGERVSWGAKALPGGGYWSMPKLSMPGAVLVGDAGGMVDTAALKGVHHSINSGIL
                     AAEAIYESLKQGSDDFSAYETAIEESVTGKELWEVRNTRQPFSKGFFLGGALVNLMIA
                     TKGKIPVRLPWHRQDEREMFIGKTSKKYPKPDGKYTFDKLSSVFITGNATRDDAPNHI
                     RVQQNVPREIAETWKWMCPAGVYEIPEDAPEEGMVDVIVNYTNCVQCGAITAKGGRLT
                     TPEGGDGPLYQNT"
     gene            complement(17563..>18410)
                     /locus_tag="VHF88_04985"
     CDS             complement(17563..>18410)
                     /locus_tag="VHF88_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2161160.1"
                     /translation="DDLTDDLCASLGNYGATKVFRVNGPEGLGAPVVDAMAKVIQDNG
                     YSYALFGGGLLGFEIGAGLTARLNAGVTMEVTAVKAQDGKLVAERPILQDSAIVDVDY
                     VGEPGIIIGRLNAFDVVESGGGAAEVVDVDVAFEEHSGRAVMVQRGEQRGADVNIEDA
                     DILVGGGRGLGKAEGFQLCEDLAGALGGAVAATRAVVDAGWYPYAAQIGQTGKTVAPK
                     LYLAAGISGAIQHKVGMQASENIVAINKDANAPIFEFCDLGIVGDLNAIMPKLTEAIK
                     ARKGG"
BASE COUNT         2751 a         6504 c         6518 g         2637 t
ORIGIN      
        1 ggcctttcta agccgccttt gcgacttgct ttctaagtct ctcgagccgg ttgcgcacgc
       61 ttgcgtccag caccatgttc ccgacctgga gcaccagacc gccgagcacg tcggggtcga
      121 cgcggctggt cagctcgacc cgctcgccgg tctgctgctc gatctgcctg ccgattccct
      181 cgacgagctg cgagtcgagc tcgaccgcgc tagtcaccga caccggcagc agcttgttct
      241 cgcgccgcca gagccggtcc agctcggccc tcaccctgaa gatagcgggc atccggtggc
      301 gctccgcgag cagctcgagg aagcggacga agttctcgtc ggcgtcctcg accagcgccc
      361 cgatgccgtc cttcttctcc tgcgaggaaa agtacggcga gaagaagaag acctggaggt
      421 cgcggctctc ggagagcacg tcggcgaact ggccgagctc ctcgtgcacg cgatcgagga
      481 cgccctgctg ctgggcgacc tcgaacagcg cgcggctgta gacctcggcg atctcctcca
      541 tggcctagga gcgtccctcc gagccggcca gcgcagagaa gtcgacctcg ctcagcgcct
      601 cctcgatcag gcgccggtgg tcggcgtcgt cgagcgactt gcgggtcacc ttctcggtcg
      661 cgatgaccgt caggtcggcg acctccttgc ggatctcgtc gagcgcgcgc cgggtctcgg
      721 cctcgatgtc cttgcgcgtc gtcgcgagca gctcctcgcg gtactccttg gccgcgccct
      781 tgacgtcctc catgtgcgag tcggccgcct tgcgtgagcg cgcgacgatc tcctcggcct
      841 gctcacgcgc ctcgcgcagc cgcgcgcggt actcctcgag cagctggtcg gcctcctcct
      901 tggtgcgcgc ggcggcgtcg atcgactcgt cgatcgcccg gcgccgcttg tcgagcgcct
      961 cgccgatcac cgggaacgcg aacttggcga ggatcgccat cgagatgccg aacgcgatca
     1021 gcgtccagag catcagcccg acgtcgggcg atacgaggaa gctgccgccc tcgtcctcgg
     1081 cgccggcctc ggtggccgcc aggacgagcg tcgcgatttc ggtgaatgcc tccacgatca
     1141 gctccctatg cgaggaagaa tgcgatcagg ccgaagatga acgtgtagaa ggcgaccgcc
     1201 tcggtcaacg cgaagccgag ccagcgaatc gactgcagct cggccgccat ctctggctgg
     1261 cgggcgaccg actcgatctc cttgccgaag atgatgccga tgccgatgcc ggcgccgatc
     1321 gagccgaggc cggcgccgac gccgagggcg atcgcgcggc ccgcgtcggt gatggcctga
     1381 gcgtcggccg actgggcgag tgggagcagc aggtccactt gttctcctta gtgactgtcg
     1441 gcggttgctc cgccgaggta gatggaagtg aggatcgcga agatgaatgc ctgcaacgtc
     1501 gcgaccaggc cgatctcgaa gaggaagaac gggatcgcca tgccgagcgt gatcacgccc
     1561 agcgcggaaa ttccgagcag cacgacgagt ccaccggcca tgaacaggat cagcaggtgg
     1621 ccggcgagga tgttcgcgaa cagccgcacc gacagcgaga tgatccggac gatgtcggag
     1681 atcacctcaa tcacgaagag cgggatcgcg ccgaccccgg acatcccggg cggcacgaag
     1741 ctcttcatga agccgacgaa gcccttcacc ctgatgccct cgatgtggta ggcgatccag
     1801 acgacgagcg tcagcgcgag cgggatcgag aggttggcag tcgcggcgta gatcgccagc
     1861 gccgggacct cggccccgaa gatgttgacc gggtgctcgg tgttggtcgg cagcggcagg
     1921 tagccgagca tgttcgagaa ccagatgaag aagaagagcg tggccacgaa cgaaaaccac
     1981 ttggtcgcca tcggccggcc catgttctgg ccggtgatgt tgttgcgcgt caggtcgtag
     2041 gcgacctcga tcgccgtctg gacgcggttc ggacgcgcct gcatgcggcg ggcgatgaag
     2101 accatcgtcg ccgtcgtagc ggcggcggcg aggaagaggt acatcaccgc cttgttgatg
     2161 ctcatgtcga tgccgaggac gttcagctcg atccacggct ggagacggaa ctcctcctgc
     2221 ggctggaaag cctcgttgcg cccctcgctg ccgaagagca cgccgaacac gaccgcgagc
     2281 aggagccaga ggccgaggcc gatgaagacc ttggtgcgcg tcctcatcgg cgcgctccgg
     2341 cgaccggtcc cgccgcgagc tgaaggacca gcgcgcagct gaataggacc gcggcgagga
     2401 tcgccgccgg caggcccgcg tcgcggtcgg cgagaccgac ggcggcgacc gacgctgtga
     2461 cgagcatgag ccggatcagc atcgacaggc cgaccacccg catcaccctg gcccggtcgc
     2521 cgctggcgag cgcgcggcgc ttcgccagcg cgatgatcca gcgctgcgcg atccaggcca
     2581 cggcgaccgc ggcccatccg agcagtggga ggcccgcgat caggaacagc ggcagggcca
     2641 gtgcgagcac cagcaggtcc aggccacgga gcagggtgag gaggaaacca gcctctcccg
     2701 acccctcggg tgttacgact gcgtgagcgg tcaaggggcg agagactcta taggaatggc
     2761 ttgttaaagg cctttgtggc gaaaatcaca ccgctgttgc aagccgtcac accacgcgca
     2821 ggattgcctc gacgactcgc tcgtcgaact ggcgaccggc accgcggcgc agctcctcgg
     2881 aggccgtcgc ggcatcgagc gccggccggt acgggcgcgc ggcggtcatc gcctcgtagg
     2941 cgtccgcgac ggcgaggatc gacgcctcga gcggcacctc gccggccgtc cgcccggccg
     3001 gatagcccgt cccgtccggg cgctcgtggt gcgcgaggat ccactcgccg atgtcgtcga
     3061 agtcggtggt gtcgagcatc cgcgcgccga tcgcggggtg cgatcggatc catgtccact
     3121 cgtcggcggt gagcggcccg cgtttgcgca gcagctcgtc gggcacgccg acgcggccga
     3181 cgtcgtggag gatgccggcg atccgcaggc gctcgacggc atcgggcggc agcccgagct
     3241 cacgggcgat cagctcggcg tagcggccga cgcgctggct gtgcgtagcg ctgcccgagt
     3301 cgcggacgtc gagcgcctcg gcgagcccga gcagcgtcac cagctcgacg tggacctcgt
     3361 cgcggccgtg cggggcgcgc gccaggatgc cggggacctc ggcgctcgag atgacggcac
     3421 ggttgcgacc gaggcgcttg gccgcgtaca gcgcttggtc ggcggcctgc aggagcgcct
     3481 cgccggtctg gccgtggatc gggaagctga cgatcccgaa gctcgccgtc agctgtcccg
     3541 cgccaggcgg ctcgaacgtc tcctcgatct ccactcgcag ccgctcggca agcacgtacg
     3601 cgccgtgctc gtcggtgtcc ggcgccacga tcgcgaactc ctcgcctccg attcgcgccg
     3661 cgacgtccca gctgcgcttc atcgcgcgga tcgtctcgcc gacgcggcac aggacgtcgt
     3721 cgccggcagc gtggccgaag cgatcgttga gctccttgaa gcgatcgagg tcgccgacga
     3781 tcacgctcag cccgcggtcc gcccgccggg cgcgctcgag ctcgacgtcg aaggcctcct
     3841 cgaaaccgcg ccggttgagc agaccggtca acgggtcggt ccgagcggcg tcggtgaggc
     3901 gcgagatcat gccggtcagc cgatcgcgaa tgacgccgac gacgagaccg gcggtgctca
     3961 acgtgacgat cgtcgcgacc cacgagtcga tcggcgtcca gtcgaggtcg cgcaagccca
     4021 gctccacgcc gaagagcgct cccaacaccg ccgtatggac gagggccgcc ggcagcggga
     4081 agaagtagaa ggcgtagacg gcgatccaca ggtacggcag cgggccgtac ggggcctgat
     4141 cgccgaagct gtagatcgcc accgtcgcga gcagcgtcgc cagaaccgtg ccggcgtgaa
     4201 agcaccagag cgggatccgg tcgaacccga tcagggcgat cgctgcgagc gtcgccgagc
     4261 ccaccgccac gaccacgagt ccggttacgt cgatgtccgg gccctgcggc ggcgccagga
     4321 cgagcagcgc gagggcggac ccggcgagcg cgaaccagaa cgctgtccgg gccatcgccg
     4381 cgcggccgcc gggcacgttc gccgccgggt cgttcgatgt cttccagccg aggcgctcgc
     4441 gaaggggcgg atccatatcc gtgctcagtt ctcccgcggc gcctaccccg tgcgggatgg
     4501 cagcccgctc ggggtttgca agatctcgtc caatataggc ccgcctgccg atatgcgcca
     4561 atcggccagg ggccccactg gcaagccgca gggcgcgccc ggcggctagc gttgcagccg
     4621 tgtgggtcag ccgcgatctc gtgctcgagc cccgtccgcg cggcttccac ctgatcacgt
     4681 cggaggtcgt cggggcgctc cccgagctgc gccagctggc ggccggactg ctccaggccc
     4741 agatcctcca tacgtcggcg tcgcttacgc tgaacgagaa cgccagcccc gacgtccgcg
     4801 ccgacttcga gtcctggttc acccgtgcgg tcccagagga cgctccgtac tggcgccaca
     4861 cgctcgaggg ccccgacgac atgccggcgc acatcaaggc gtcgctgttg ggcccttcgg
     4921 tgaccgttcc gattcgtcac gggcgcccgc tactcgggac ttggcagggc atctacctct
     4981 gcgagcaccg cgaccgcggc ggcccgcggt cgctggcgct gacgttgacc ggcgagtagg
     5041 gcatttcgtc tctggcggct cgccgcatgg tatgcgaacg tatgttcgca tgcgatggga
     5101 cgcgctcagc atcgaggacg aggcgacgcg acagctgccg ggctaccgcg agccggccac
     5161 cgttcggacc ttcgacgcac cggaggcgat ggacatccgc ttctatgagg ttcgcgcgaa
     5221 gtcggcgctc aaccgcgtgc ccgaggcctc gcggatgccg ttccgctgga cggtcaaccc
     5281 ttaccgcggc tgcacccacg cgtgcgtcta ctgcctcgac ggcggaacgc cgatcctgct
     5341 gttggacggg acgacccggc cgctgtcgga gctgcgaccg ggcgaccgga tcgtcggtac
     5401 ggagctggtc ggcgctcgcc gccgctacgc gatcaccgag gtcctcgcgc actggcagac
     5461 gctgaagccg gcctaccgcg tggcgctctc cgacggcacc gccctggtgg cgagcgccga
     5521 ccaccgcttc ctcacacgcg ccggctggcg acaggtcacc ggcaacgagc cgggagggcc
     5581 gcggcgccgt ctcagcgcca gcgaccagct gctcggcccg ggactggccg ctggccagac
     5641 ggcgacggcc ggcgagccgg tcgagtcctc gcgcgggctc tgggtcgact cgatcgagcc
     5701 gctcggcgag gagcgcgtgc tgttcgacat cacgaccggg accggggact tcgtcgcaaa
     5761 cggggtggtc agccacaact gcttcgcccg cccgacgcac aagttcctcg acctctcggc
     5821 gggcagggac ttcgagcgcg agatcgtcgt caaggtcaac gccccggagg cgctgcgcgc
     5881 tcagctctcg cgaccgtcgt ggaagcgcga gcacgtcgca ctcggcacca acacggaccc
     5941 ctaccagtgg gtcgagggcc gctaccgcct gatgccgggc atctgggagg cgctgcgcga
     6001 cagcggcacg ccctgctcgg tgctgaccaa gtcgccgctg ctgctgcgcg acatggcgct
     6061 gatgaaggag ctcgtcgagc aggtcggctt caccgcgagc ctgtccgtgc cgacgctcga
     6121 cgagcgggcg tggcgtgcga ccgagccgca cacgccgcat ccgcgcgcgc ggctggaggc
     6181 ggtcggcgag ctgaaccgcg ccggcgtgcc gacaggcatc ctgatcgcgc cgctgatgcc
     6241 gggagtcaac gactccccgg agcaggtcga gcggatcctc gagctggcga ccgaggccgg
     6301 cgcgacgagc gtcggcggcc tgcacctcca cctgcgcggc gaggtgcgcg ggatcttcat
     6361 ggactggctg cgctccgagc gccccgacct cgtgccgcgc tacgaggagc tctacgcgcg
     6421 cggcgcctac gctccgcgcg ccgagcgcga gcgcgtcgcc gcgctcgtcg gccgacgcgg
     6481 accgaagtgg ccgcgacgcg gaacgctgcg gccgcgacgc gagcctcccg gacaggacga
     6541 cgacgggttt gagcagccct cgctcttctg atccgacatc ggacctcagg ccccccgtac
     6601 cccccgcggc gccgggccgg tcgaaggagg gctgacggcg tcgaactcgc ccgtctcgac
     6661 ctcgcgctcg atctcctgct cgatctccgc ctcgctcaac gagggaccct cacgccgcag
     6721 ctggcgctcg cggaaacggc ggaacttgag gatctcgagc acgagcacga ggtagaggct
     6781 cgcggcgagc gcgatgagcc cgaatgcggc catcacgacc gtccagccga ggttgaagct
     6841 gccgccgtcg tccgagtagg gcacgaagcg cagggccagc gccagcgcgg ccagcgacag
     6901 cgtccaggcg tagaggtaga gcaccgtgcg ccgctgcgag aagccgatgt tcgcgaagcg
     6961 gtggtggaag tgccaggtgt cggcggagta gacggggcgg cggtacttga tccgcttggc
     7021 tacgacgaac gtggcgtcgg cggccggcac ggcgaggatc acgagcggga agaacagcgc
     7081 gacgaccgcg gcagtcttca gcaccccctg gatcgcgacg caggcgagca gcaggccgag
     7141 caggttcgac cccgagtcgc ccatgaagac tgaggccgga tggaagttgt ggcgcaggaa
     7201 gccgaccgcg gcgccggccg tggcggccgc gaggatcgcg ggatcgacgc ggtcgagcga
     7261 gagtgcgatg accgcgaacg tcgccgcggc gatcgtgcag acgccggcgg ccagcccgtc
     7321 gaccccgtcg gtgaagttga cgacgttcat caccgcgacg atcccgagca gcgtcagcgg
     7381 gtagccccag tcgccgatgt cgagcggatc gacgaacggc agcgtgatgt tctcgacgcg
     7441 gacgtcgcag gccaccggga tcagcgccgc ggcgagctgg ccgagtagct tcaggacggg
     7501 gttcaggccg ccgggcttgg cgtcgtcgac cgccccgacg agggtgatcg ccgcggcgcc
     7561 ggcgaggatg ccgcgcgtcg cctggtcgcc gggcaggaac agggcgcccg cgagcagcac
     7621 gcccgccagg atcgccagcc caccgagccg gggcatgggg gcatcgtgga ggtcgcgctc
     7681 gcgcggcagc gcgaccgccc cgacccgcgt ggcgagccgg gcggtgagcg gcgtcagcag
     7741 cagtgcgaca gccgccgcga cgaggaacgc ccagagcgcg tcgaggtcgt tcatccgggc
     7801 cagcctactg gcggcccagg tcagccctgg ggcaggaccc agaaggcgat gacggcgtac
     7861 tgcagcgcgg ccgcgacgat cacaagggcg tggaagacct cgtggtagcc gaagaccgcc
     7921 ggcacggggt cgggccggcg cagcgcgtag gtgacggctc cgagcgtgta gagcacgccg
     7981 cccagcgcca ccaggatcga ggccaccact ccgacctggc tgatcagctc cggcgtgacg
     8041 gcgaccgcga cgaagccgag cgccacgtag atcacggccg ccagccgctt cggcgcgtcg
     8101 atccagagca gcttgagcag gatccccccg accgccccgc tccacatgac ggcgagcacg
     8161 acgtcggcca gcgagccgtg caaaaccaac agcgcgaacg gcgtgtaggt gccggcgatc
     8221 agcaggaaga tcgccgagtg gtcgagccgg cgcatccagc ggcgcgcgct gtcggtctgc
     8281 caggtgacgc ggtggtagag ggcgctcgcg ccgaacagca gcgtcacgca cgcggcgtag
     8341 atcgccgctg cgagcgtgcc cctgccgggc ggcgcggtaa cgacgagcgc gacgccgagc
     8401 gcgagcgaga tgaagaaggc ccactgatgc gtcacgccgc gccagcgcgg acgctcgcgc
     8461 agcgcctgtc cgatgtcgcg cttggcctgc tcgaggtcga gcctgggcgc cgtctccgcc
     8521 atggcgtcca ggctagcgga ggagcgcagc cggatcggtc ctacgaagcg gcgtcgcagc
     8581 taggtggagc gagatccggc gagcaccgca gctctctcct cagaccgtgg cgacggcctt
     8641 gcccagctcc ggataaaggg ggtagcggtc ggcgagcgcg cgcgtgcgct ccgcgagatc
     8701 ggcccgcacg ctctcggtga agtcggcggt cagagcggtg gcgatcacct tgccgacctc
     8761 ccggaagtcc tcgagctgga acccgcgcgt cgcgagggcc ggagtgccga tccgcagtcc
     8821 cgacgagacc gccggtggcc gcgggtcgtt ggggacggag ttgcggttga cggtgatgcc
     8881 gatcgcatgc agccggtcct cgccctgttg gccatcgagc tcggagtcgc gcaggtcgac
     8941 gagcaccagg tggacgtcgg tgccgccggt aaggacctcg acgccagctg cggtcagctc
     9001 cgcggcgatc gcggcggcgc cggcgcgggt gcgctgctgg cgctcccgga acagctccga
     9061 ctgcgcgacc ttcatcgcga ccgcctttgc cgcgaccacg tgcatcagcg gcccgccctg
     9121 ctggcccggg aagacggccg agtcgatctt cttggcgtgc tcctgacgcg cgaggatcat
     9181 gccggagcgc ggcccgccga gcgtcttgtg gacggtcgtc gtgacgacgt cggcatgcgg
     9241 cacggggctc gggtgctcgc cggccgcgac gaggccggcg aagtgggcca tgtcgaccat
     9301 caggtaggcg ccgacggagt ccgcgatgtc acggaagcgg gcgaagtcga gctggcgcgg
     9361 ataggcggac cagccggcca agatcagctt tggccggaac tcctgggcga gccgctctag
     9421 cacgtccatg tcgacgcgcg agtccttgtc ggagacgccg taggcgacga tgtcgtagag
     9481 ccggccggag aaattgatct tcatgccgtg cgtcaggtga ccgccgtggt cgagcttcat
     9541 gccgaggatc cggtcgccgg cctgcagcag cgcatggaag gcagcggcgt tggcctgggc
     9601 gcccgcgtgc ggctggacgt tgacgtgctc ggccccaaag agctccttcg cgcggtcgat
     9661 cgcgaggcgc tccgccacgt cgacgtactc gcagccgccg tagtagcgct tgccggggta
     9721 gccctcggcg tacttgttgg tcagcaccga gccctggcag tcgagcaccg cctgcgggac
     9781 gaagttctcg gaggcgatca tctcgagcgt cgtctcctgg cggcggagct cgtcgcccag
     9841 cacctgggca atctccggat cgacctcgga tacgggcttg gagaagtagt cggggctcag
     9901 atcggtcacg ctggccacgg taccagcggg gggccggcgc cagattcgcg atttgccatc
     9961 gagcgaagcg ttgtgcggcc tatagccaga ctaaggcttc aaccggtgtc gcgaagcagc
    10021 cgtgcgaggt cgccggccgg cagcgcgccc tcgcgcagga cgtcgaagcg cccgtcgtgc
    10081 tcgtactgcg acaggtcgac gaccgtcgat gcgacgccgg gcaggtcgcc gccgtcgagg
    10141 gcgaggtcga cgcccgcgcg aatctcctcg ggcacgtcgg cgagccggcg cgggtccgcg
    10201 ccgccatgga ggttcgcgct cgactggagc actggcacgg caaccgccgt gagcggctcg
    10261 agcggcccgt cgagcgctgg cactcgcacg ccgaggcggc tcgtgtccgg accgcaggcg
    10321 agcgggaagc gcccgcgcgg attcgcgacg atcagcgtca gtggtcctgg cagcagccgc
    10381 tcgaaggcgg tccgtgtgcg tgccccgagt tccggcagcg ccgccagggc gggccgtagc
    10441 gcgaagaaca tgaccgcgct gggctgcgag gcctcccgac ccttgagctc ctgcagccgg
    10501 cgaacggctg gctcggagtc gggagacgct gccaggccgt agacagtgtc ggcggggaag
    10561 acagctatcc ctcccccggc gatgcagcgc tcgaacgcag cgacgtcgcc ttcgccgaga
    10621 ggaccagccg ctccggcctg ccgtgccgcg tggctgccgc tcacgtcacc gtccccacgg
    10681 tcacgcgctc gcggccggac aagtcggatc gcgtctcggc gccggcgagc agcgcccgca
    10741 cctcgctgcc ctgctcgcag ccatgctcga gcgccagccg cgtcccgcgc ggcgcccgcg
    10801 cgacgagcgt gcgaatcaca tcgagcccgt ccgcgccggc cgtcagggcg ctccgcggct
    10861 cgtactgcat gatctccggt gccagcctgg tccactcgcc gtctgtcacg tacggcaggt
    10921 tggcgactac caggtcgtac tcacccgccg gcaggccgtc cgcgacgaac ggctctagcg
    10981 gcaggccgag gcgagcggcg ttggcgcgcg cgacctccac ggcactgggc gacacgtccg
    11041 aggcggtcac gcgcaggtcc gggcgctcgt cgagcagcgc aagtgccaac gccccgctgc
    11101 cagtgccgac gtcatgcacc gccgccccgc tcggcagctc gagcgcgacc tcgaccagca
    11161 gctcggtctc cggccgcggg atcaacacgc gccggtcgac cgccagctcg atgcggcgga
    11221 agcccttccg cccaacgatg tacgcgaccg gctcgcgtgc gacgcgccgg cggatccgct
    11281 ccccgatctc gcgcgcgctc gccggtggta cggcgagctc cgagtcggag atcagcgccg
    11341 cccgatcgac gccgagcgcg tcggcgatca ggacctccgc atcgagccgc ggcatctcgc
    11401 aaccggcggc ggcgagcgcg tcgacggcgg cgtcgaccgc atcgcgcgcc gtcatcgacg
    11461 ccgcgggcat cagctctcga cgagcgagct cgcctcgagc cgctggcgct tctcctcggc
    11521 ctcgagcgcc gccgtgaagt cgtcgagctc gcccgccagg atgcgctgga cctccccctt
    11581 ggtcaacttc acgcggtgat cggtgacgcg atcctggggg aagttgtagg tgcggatctt
    11641 ctcggagcgc tcgccggtgc cgacctgcga gcggcggctc tcggcgagct cggcctgctg
    11701 cgcggccagc tcgcgctcat agaggcgggc gcgcaggacg cgcatcgcgc gctcgcggtt
    11761 ctggagctgc gacttctcgt cctgcatcga gacgacgaca ccggtcggct tgtgggtgat
    11821 ccggaccgcc gagtcggtcg tgttgaccga ctggccaccg ggccccgagg agcggtagac
    11881 gtcgatttgc aggtcgttct ggtcgatctc gacctcgacg tcctcgacct ccggcagcac
    11941 cgccaccgtc gccgtcgagg tgtggatccg cccctgcgac tcagtctcgg gcacgcgctg
    12001 cacccggtgc gtgccgccct cgtgcttgaa gacgctgtag gcgccctgtc ccttgatcgc
    12061 gaaggtgtag tccccgtcgg cgacgttcat cgtctcggtg ttgaagccga gccgctcggc
    12121 gtagcgggtc agcatccggt agaggtcgcc ggcaaacagc ccggcctcct ccccgcccgt
    12181 cccgccgcga acctcgacga tcacgttctt gtcgtcattg ggatccggct cgaccatcgc
    12241 caagcggatc tcctcgtcga gcgccgcgag ccgctcctcg gcggtctcga gcatcgcacg
    12301 gatctccaga tcctccccgt cctccgacag catctcccgt gcgcccgccg cgtccgaggc
    12361 cgccgttcgc cactcccggg ccagctcggc cgccggctcc agggcgctgt acgcccgacc
    12421 gacctcggcg tagcgctcgc ggtccccgat cacctcgggg tccgacatct gctccgacag
    12481 ctcggcgaac cggctctcga tctgctcgac cagcttctga atcacagtcg aagcgtacga
    12541 cgtcgctagg cgccgacctc cataacgacc cgctccgcct cgctgacgtc ggcggggtcc
    12601 tcgaactgga gcacggcctc gagggcggcg atcgcaacgg cgagctggga gagctcgggc
    12661 tcgcgcgtgg tcaggcgctg aagctggagg ccgggccaca tcaggacctg cgcccaccgc
    12721 ttggcgcggt tgcgcccgaa ccacttgatg acctcgaagg cgagaccggc gacgatcggg
    12781 atgccgacga cgcgcgagat caacagccac tgccagtcga gctttccgag cggagcgaag
    12841 acgaagatgg ccacgatcat cacgatcagc aggaagctgg tgccgcagcg cgggtgcagc
    12901 cgggagaagc gctgggcgtt ctcgggcgtc aggtcgaggc cggcctcgta gcaggagatc
    12961 gtcttgtgct cggcgccgtg gtactggaac acgcgctgca gatcgcgcat ccgggagacg
    13021 agccacaagt aagcgaggaa gaccccgatc cgcaccagct tctcgatcac cacgaagacg
    13081 acggagttgg ggatcgagtc cttgaagagg ttggtcgtga acgccggcag caggaagaag
    13141 agcccgatgg cgaagagcag cgcgaacgcg atcgtccctg cccacgtcgc gccgccgatc
    13201 tcttgggtct cgcctcggtc gtccggcgcc agctgggcgt tggcggagat gccgagcgcc
    13261 ctgaagccga tcttcagcga ctcgtagagc gcgaccatcc cgcgcaggac cggcaggcgc
    13321 gcccagcggt gcgtcttcgc atacgagacg agcggctcgg tgacgacctc gatctcgccc
    13381 ttcgcgccct cggtggcgtt caggccgccg gtctcgagct gctccggggt cggcttgcgg
    13441 atcgcgaccg cccacgtcga ggcgccgcgc atcatgaccc cctcgagcac cgcctggccg
    13501 ccgatcggca ggtcccgttg agcctgcagc tgttgctcgc cgctcacccg cgagatcgta
    13561 acggccggcc gttgccgcgc cgcgtgctca gtccatcgcg gcgcacgcga acgagcgggc
    13621 gttgtcgcgc tcgttgcgct cgcgcaaagc gtcgcccgga tcgacctcga cggtgacatc
    13681 gcgcgcgcat gcgggaccga cgaaggcgac gtcgcgccgg gcgtgtgccg caactcggcg
    13741 cacgccgcgg gtgtcgacct ccgcgccgtc gacccgcagc gcgatttcga gtcgccgcgc
    13801 cggtgccgcg ccgcggttga cgaccgtcgc gacgtagcga acggtctcgg cggtcggccc
    13861 ggcgagcgtc tcgagccccg cgacgcgaag gttcggcagc ggccccgctt ggcggcagac
    13921 cggcgagcgg gccgcctggc gctcgatcag catcccggcg gcgtcgtgcc actggaactc
    13981 gacgacgacg cggtaggcgg ttccgcgggc cagagccgcg atccgctggc ggtaggcgaa
    14041 gcgcgcgacg tcggggcgcg cgtggcgcca gacaccgagg ccgggcgcct cgacggcgcg
    14101 ccaggcgttg cggccgagac gctccctcag ctcgaagcgc atgcgcatgc tggcagcgtc
    14161 gggaagccgg ctcatctcgc cgcgaaagag ggcgttgcgg tcggcgaccc gcttcccgcg
    14221 ctcgcactcg agcagctcga cagagccgtc cgccgcggga gccggcgcac cgctcacgct
    14281 ggcggtggcg caggccaggg cgacaacggc cgcgaggcct gccagcaaga cgatcgaaac
    14341 gcgactgctc atcggctcag ggaacagccg ccgaccgacc gagttgcggt cggagctggc
    14401 gctcaggcgg cgccggcgcg accgcgcgcc tggcgacggc ggaagcgctc gacgcggccg
    14461 ccggtatcga tcagcttctg cttgcccgtg tagaacgggt ggcagttcga gcagacctcg
    14521 acgtgcaggt cgggcttggt cgagcgggtc gtgaactcgt tgccgcaggt gcagcgaacg
    14581 tgggcctcga cgtagttggg atggagatcg gtcttcatca ggaacgcacc agtctagcgg
    14641 aggagcgcag ccggaccggt cccacgatcg cacgtcatag ctcgcgggag cgaggtccgg
    14701 cgagcaccgc agctacggtt tccgtccccc gagccagtcg cgcatacgcg cccaccaagt
    14761 gcgcggtcgc gcgaaacgtg cgtgcggcgg ctgcttggcg cgcacgagcg cgatccagtc
    14821 gtcgagcggg aggtcgccgt cgatcagccg ggcgagcgcc gaggtgaccg gcgcctcgag
    14881 gccggcgcgc tcgatcgccc gtgccagcgt gcgcacggtc tcgagcgcct cgaccgcctg
    14941 gccgagccgc gccgggatct cggccgcagg cgtcccggcg gccagcagct cgccggcgct
    15001 gcggttgcgc gacgtcggtg cgagcgcggt cgcgacgagg tccccggtgc cgctgcgccc
    15061 gacgaaggtc cgtgccgagc cgccccacag ctcggcgagc gcgagcacct cgaggaagat
    15121 gtccgccgcc gccatgccgg cggcgttgag cccctgcgac tgggtggctc cgacggctac
    15181 cgcggcggcg ttcttcgcga cgcccgccag ctcgactccg accgggtcgt ccgaggcctc
    15241 gcagacgact cccgcgcgct ggaagagccc ggacacgcgc agcgccagcg cctcggagcg
    15301 cgaagcgcag accagcccgg cgccggtctc gaccatctcg cgcgcatgcg ccggcccccc
    15361 gagacaggcg gtccgctcgg cgccgaacgc gcgctccagc gccgcggtcg gcggcgtccc
    15421 ctcgggcggc acgaggccct tggcgagcga caccatgccg gcatgcggcg ctacgccgag
    15481 ccgctcgatc tcggcgatcg cctcgcccag gctcttcgac ggcaccgcga ggaagaccag
    15541 gtcggggcgg cgcagctggt cgtcgtgggc gccgaacgcc cggatcttga gcgcggcagg
    15601 cagctcgacg cccgacaggt agcgccggtt ctcgcgcgcc tcggtgagcg catctgcctg
    15661 ctcaccggtg cggcagagca gggtcgtgcg cacgccggcg cgctcgagca gcagcgcgat
    15721 ggcggtgccg aacgaccccc cgccgacgac cgaggcgcgg cgaggggcgt ggctctggtg
    15781 acggcggctc ggcgcgatag ccggacgcta ccgaccgtca ggtgttctgg tagagcgggc
    15841 catcgccgcc ctcaggcgtg gtgaggcgcc cgcccttggc ggtgatcgcc ccgcactgca
    15901 cgcagttcgt gtagttgacg atcacgtcga ccatgccctc ctcgggcgcg tcctccggga
    15961 tctcgtagac gcccgcggga cacatccact tccacgtctc cgcgatctcg cgcggcacgt
    16021 tctgctggac ccgaatgtgg ttcggcgcgt cgtcgcgcgt cgcgttgccg gtgatgaaga
    16081 cgctcgagag cttgtcgaag gtgtacttgc cgtcgggctt ggggtacttc ttcgacgtct
    16141 tgccgatgaa catctcgcgc tcgtcctggc ggtgccacgg cagccgcacc gggatcttgc
    16201 ccttggtggc gatcatcagg ttgaccagcg ctccgccgag gaagaagccc ttcgagaacg
    16261 gctggcgcgt gttgcggacc tcccacagct ccttgccggt gaccgactcc tcgatcgccg
    16321 tctcgtaggc cgagaagtcg tcgctgccct gcttgagcga ctcgtagatc gcctccgcgg
    16381 ccaggatccc cgagttgatc gagtggtgga cgcccttcag cgccgcggtg tcgaccatgc
    16441 cgccggcgtc gccgacgagc acggcacccg gcatcgagag cttcggcatc gaccagtagc
    16501 cgccaccggg aagcgccttc gcgccccagc tgacgcgctc gccgccctcg aggatcttgc
    16561 ggaccagcgg gtgcattttg aaggtctgca gcaggtcgtg ggccgaggtc gtggcgtcgg
    16621 cgtactcgag gtcgatcacg aagccgatcg acacgaggtc gtcgccggtc ttctcgtcct
    16681 tcatcgggta gagccaggtg ccgccggtct gcccgtactt cgccgaggtg ctgaccggcc
    16741 aggggccgac ggtgtggatc agccgatcga gcggcttcgg aaccttccag acctccttga
    16801 ccccgagctc ccaggtctgc ggctcacggc cctcgccaag gtcgaactcg cggatcgccg
    16861 gcccggtcag atgtccccag cagccctcgg cgagcaccgt cgccttggcc ttgatgtcgg
    16921 tgccgggctc aaagttgccg agcggctcgc cgtccttgcc gcggcccttg tcgccggagc
    16981 ggacgccgac gaccttgccg tcctcgacga tcagctgcgt cgccgcggtc tcggtgagca
    17041 agtaggcgcc ggcctcctcg gcgatccgct gctggtagcg cgcgagcgcc gagaccgaga
    17101 tgacctcgtt gccgtggttc ttgaatggcg gcggggtcgg gatccgcagc gcgcgcttgg
    17161 aggtcggcgt catgtagacc gcctccttgt cgacctcgcc gaaggcgaag ccctccttgc
    17221 gccagtcctc gcgcgtcatg tccgggaaca gccgctgcag cggctccggg cgaacgaccg
    17281 cgccggacag gttgtgcccg ccgcgggtct tggccttctc gatcaccgcg accggcacct
    17341 cgccgaggct ctccatcgtc tccgggtcgt ccgcgagcag ctgcagcagc cggttggcgc
    17401 aggccagccc ggcggtcccg ccgccgacga tcgcgacgcc gatctcgatc agctcgtcct
    17461 cgggatcgag tccgcgcttg atgaactcgt ccggggactt gacgggaggt ggaaaggccg
    17521 ccggagcgac cttcccattg ccattcgcgg aggacatcgg ggtcagccgc cctttctcgc
    17581 tttgatcgcc tcggtcagct tcggcatgat cgcgttgagg tcgccgacga tgccgaggtc
    17641 gcagaactcg aagatcggcg cgttcgcgtc cttgttgatc gcgacgatgt tctccgacgc
    17701 ctgcatgccg accttgtgct ggatcgcgcc ggagatgccc gcggcgaggt acagcttcgg
    17761 cgcgacggtc ttgcccgtct ggccgatctg cgccgcgtag gggtaccagc cggcgtcgac
    17821 caccgcgcgg gtcgcggcga cggcgccgcc gagcgctccg gcgaggtcct cgcagagctg
    17881 gaagccctcc gccttgccga ggccgcggcc cccgcccacc aggatgtcgg cgtcctcgat
    17941 gttcacgtcg gcgccgcgct gctcgccgcg ctggaccatc acggccctgc cggagtgctc
    18001 ctcgaacgcg acatctacgt cgaccacctc ggccgcgccg ccgccgctct cgacgacgtc
    18061 gaacgcgttg aggcggccga tgatgatgcc cggctcgccg acgtagtcga cgtcgacgat
    18121 cgccgagtcc tggaggatcg gccgctcggc caccagcttg ccgtcctgcg ccttgaccgc
    18181 ggtgacctcc atcgtcacgc cggcgttcag ccgcgccgtc agaccggcgc cgatctcgaa
    18241 gccgagcaga ccgccgccga acagcgcgta ggagtagccg ttgtcctgga tgaccttggc
    18301 catcgcgtcg accacgggag cgccgagacc ctcggggccg ttgacgcgga agaccttggt
    18361 cgcgccgtag ttgccgagcg aggcgcagag gtcgtcggtc agatcgtcgc
//
LOCUS       DATABV010000118        14697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1965365, whole genome shotgun sequence.
ACCESSION   DATABV010000118 DATABV010000000
VERSION     DATABV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14697
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965365"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1222)
                     /gene="ychF"
                     /locus_tag="VHF88_04990"
     CDS             complement(119..1222)
                     /gene="ychF"
                     /locus_tag="VHF88_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX2161161.1"
                     /translation="MKVGIVGLPNAGKSSLFNALTQAGAEAANYPFTTVEPNVAVVPV
                     PDERLDAVVDTVKATPAVYETIEFHDIAGLVRGAHKGEGLGNKFLANIRETDALVHVV
                     RAHGDDQVIHPEGRVDPLADVETIETELLFADLEQAERRLDKVAKEAKSGEREKVAEK
                     EWVEALVAALQEGRPARTVPVPEDAPDALRNLSPLTAKPVLYLANVDEDAADTGPDGA
                     LAPPPELAAHAEGLGARAAAVSARLESELGELDDEEAAAMRADLGVAESGLATVVREA
                     WKLLGLISFFTAGEGKEGRAWAIRAGTSARRAAGKIHTDIEKGFVAAEVIGWRDLVDL
                     GGYAAARDRGKLRVEGRDYVMQDGEVMTVRFTP"
     gene            complement(1259..1765)
                     /locus_tag="VHF88_04995"
     CDS             complement(1259..1765)
                     /locus_tag="VHF88_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2161162.1"
                     /translation="MKALRRFELALLRALRTRGHGPRVERAVLLFTRAGEHGALWQGA
                     CLLGAVVDGERRPLYLRTMRTVMLAYLANIGLKYVVRRPRPALEGLPALSSTVTSLSF
                     PSAHSTTSFAAAWALTRPADGLPVIPVYALAKAMAVSRGYVGVHYPTDIAAGAAFGTA
                     IAALLDGR"
     gene            complement(1825..2046)
                     /locus_tag="VHF88_05000"
     CDS             complement(1825..2046)
                     /locus_tag="VHF88_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161163.1"
                     /translation="MANAMARTDIGFQGGQVLSLRTAEDAYRKLMDALADDAGARWHA
                     LELEDELLQLDLAQVVYVRRETGNRAVGF"
     gene            complement(2049..2459)
                     /gene="mce"
                     /locus_tag="VHF88_05005"
     CDS             complement(2049..2459)
                     /gene="mce"
                     /locus_tag="VHF88_05005"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX2161164.1"
                     /translation="MFGEIDHIGIAVEDIDAAIGLYRDQLGMREQHRETVEEQGVHAV
                     LLEVGGAHVELIAPIGPEGGVARFLERNGAGMHHVAYRTDDIDAELKRLVAAGIRLID
                     EEPRVGIRGSRVAFAHPKSTGGVLTEIVQPAEDH"
     gene            2634..3440
                     /locus_tag="VHF88_05010"
     CDS             2634..3440
                     /locus_tag="VHF88_05010"
                     /inference="COORDINATES: protein motif:HMM:NF039733.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6338 family protein"
                     /protein_id="HEX2161165.1"
                     /translation="MIGTIEALGVFLLMILPGFVCLRAFEYGRPPLRIRGALGEIGGA
                     VMISTVVWSGLYLWRGKDLLPVVVGDSGESARQRLDAFAELAGLSILIGIAIALLARL
                     GDQLSRRAALFLLKRHSRKSAQARRNNANATNDDGTPVTTVEKSGWLTRVRSGVGRWM
                     RRRLRARTLPASAWDRLMTRLANRGKPVVCKVRTSDGAEILGAFASAGYADWEADGRG
                     LLLDVEVVADPDGRIRPLPMSQGIFIPGNEIVTLSVVALPDEALSLKTDE"
     gene            3433..3630
                     /locus_tag="VHF88_05015"
     CDS             3433..3630
                     /locus_tag="VHF88_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161166.1"
                     /translation="MSDWQPDIDFIDVPVPGKIEEHGISIDAPMPDFHIANPWGVAPS
                     APPANGAAPQPSAPSATADDA"
     gene            complement(3650..5173)
                     /gene="ftsH"
                     /locus_tag="VHF88_05020"
     CDS             complement(3650..5173)
                     /gene="ftsH"
                     /locus_tag="VHF88_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX2161167.1"
                     /translation="MRLLAVLTPLAFSFEEFENWAIVWLPIIFMGLIAVVLIAMLRFM
                     PSFKMKPQEIKPQSADSTGWDDVAGVEEAKDELREVVEFLRDPERFKQIGARVPKGIL
                     LHGPPGTGKTLLAKAVAHESNARFYAQSASAFVEMFAGLGAARIRRLFKEARKSAPAI
                     VFIDELDAVGATRGNDVSGEKDQTLNQLLVELDGFGGRDGLVVIGASNLLDKLDPALL
                     RPGRFDRQVFVTPPDLEGRKRILAVHTRDKPLEGGIDLELVARQTSGLTGADLANICN
                     EAAIFAARDRRERVATTDFQAALERVVAGMQSRRVITDHEKRVVAYHEAGHALCAELL
                     PSTEKVHRVSIVPRGTALGYTLNLPEEDRYLKTRDELMDRLVVLLGGRTAEHLVFGQI
                     TTGASGDLRSVHDISRAMVTQYGMGTGLMSRELNAGDYSMSDATRRAIDDEQQFIADQ
                     AHRRALSLITEHRDLLEALAGELLAREALEREDIDRIVAEVEGEIATPERLAPLNGG"
     gene            5194..5319
                     /locus_tag="VHF88_05025"
     CDS             5194..5319
                     /locus_tag="VHF88_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161168.1"
                     /translation="MTRSIRMEGFHLDLVDEARGGPPLDDLTFGSEKERELEGSF"
     gene            complement(5316..6656)
                     /locus_tag="VHF88_05030"
     CDS             complement(5316..6656)
                     /locus_tag="VHF88_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2161169.1"
                     /translation="MPDREELERQTTRLLQRLIQFETVNPPGAEQECQEYLRGLLESA
                     GFDVELLAAVPGRPNLVARLGAPSGTDGPTLCLLGHVDTVLANPAEWEVSPWSGELRD
                     DGCVWGRGALDMKSQVAAEVAVACALAQEGWRPEAGELKLVLTADEETGATHGAQWLC
                     SQHPDKVRADLIVNEGAGAAFEYDGGRVYPVCVAEKGVYRFTLTAHGRAGHASLPRIG
                     DNALTKLAPLVSALGNARLVPEHSPEPDAFFEALGIPTNDLEAALAHVEATDPRIAVM
                     LEPLLGITFAPTMVGASEKINVIPSEASARVDCRVPPGVGEDRVRRRVHEALGWEPPA
                     AGPPPGFSLAFDETVTGNRSPIDTPLMETIRSFVASEDPEATTTATALAGFSDSHWFR
                     AAFPDCVAYGFFPQRRMDLFEAMPLVHGANERIPVDDLGLAARFFAELVQSTLR"
     gene            6807..7058
                     /locus_tag="VHF88_05035"
     CDS             6807..7058
                     /locus_tag="VHF88_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Y4mF family
                     antitoxin"
                     /protein_id="HEX2161170.1"
                     /translation="MATTIDTSEDLGRAVRAARLELGLTQDDLALASGTGRRFIVNLE
                     SGKSTVRLDAVLAVLAALGLRIELQRAGPAEGADPDARA"
     gene            7045..8277
                     /locus_tag="VHF88_05040"
     CDS             7045..8277
                     /locus_tag="VHF88_05040"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX2161171.1"
                     /translation="MRALDVWLYGRHAGRLEQVDGRMRFTYAPEYVAAGGAPHSCSLP
                     LGDHEHDREAEAFFANLLPEGEARTMVARRLGVSAGNDFGLLYEIGGDCAGAVTLLPP
                     GASPETIAERAEIRWLDEPALAAALDELPRRPLLADPDEGIRLSLAGAQDKLPVVVSD
                     GRIGIPLGRTPSTHIVKTPIARFDDTVANEALCLELARSLGLSAAAAEVRTADGREFL
                     LVERYDRRHTAAGLVERIHQEDFCQALAVPPQLKYESEGGPGLADCFGVLRVASTQLA
                     VDRLALLDAVTLNFLIGNHDAHGKNFSLLLEDGATRLAPLYDLVSTAVYAGLDRKMAM
                     ALGGEYRPDYVRRRHVERFAEQTRLNPAAVRRRMLRLAARARAAAPQVAERIDPRPVL
                     DRVLETVEQRAGWLETEL"
     gene            complement(8274..9557)
                     /locus_tag="VHF88_05045"
     CDS             complement(8274..9557)
                     /locus_tag="VHF88_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX2161172.1"
                     /translation="MTVTLPTTQSPLDNITYEDLYRRWENGNWKATEIDFSKDAEQWQ
                     SAFTEFDRKAAYWNYCLFFWGEDAVADGLSPYVDAAPLEEQKYFLTTQQVDEARHAVF
                     FNRFMKEVAGVGGNSVGDSLRTIQPELSWGFKKVFGRLEQMCDELRKDPSIPRLAAGV
                     ALYHVIIEATLAQPGQHFITSYLEDRDILPGFRAGMENVAADEQRHIGFGVKLLNDLR
                     KMDPEVPHAVADLLREVMPWTINVLVPPGWDERYIETFGFTMEDLGEQGAMSLETKLR
                     SAGMPLEELPGPPVMPLDGTPRERAIRGKTLSKAGYLGEKIGPPSRDPEHVQMLFESV
                     ASGLDVGAAREPGTIVWEFRDHEPWHVVVANGTTHAAPGTVENPTVRFQVAFEDFVDI
                     VAGRVDPGRLMLRGRFRPRGDLRWLFKSRKMFPAG"
     gene            9709..10926
                     /locus_tag="VHF88_05050"
     CDS             9709..10926
                     /locus_tag="VHF88_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161173.1"
                     /translation="MLLNQRERLFAATVAVVAEKGYEATRVEDILVKAGVSRNAFYKQ
                     FANKRECFLAALDEIAALAKPSVFDVFEQTAGTWDEKLRAMLDAFAAVIVAQPAMARV
                     GWVEVYAAGPEAVEIADRIDRSIENIVCRALTYSPERAGMPREIVGAIVGGVRKIVHT
                     RVREERTHELPGLMPELFAWMQSYHTPPERLRRPRRVPPEFTALPQEPTDPRERIMRA
                     VTELVAEEGYPAMAITEIAARASVSLTTFYAHFDGKESAFLATLADAQRQLFEATVPH
                     FLSGADWPTSVSLGARAFLGFLSTNRAMAMLGGFGVWATSPAALELRAQGVALFETLL
                     NEGYRCYPETSPIAAEGIGASLDALLFKSLQSAGPARLYELGPTATFIVLAPFVGAGR
                     AFELANQTSAGVA"
     gene            complement(11088..11483)
                     /locus_tag="VHF88_05055"
     CDS             complement(11088..11483)
                     /locus_tag="VHF88_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2161174.1"
                     /translation="MGARGIAAAALAGLALAASGCAGAETPAQLTAPPKATVVFGDGA
                     YRPAHVRIPVGGRVTFVNPTTELNTAETGGIDTFELDRARLDRQERFDTHVLRTGEAE
                     SVEFDTPGTYEFHSSLDSRMKGSVTVVRP"
     gene            complement(11483..12520)
                     /locus_tag="VHF88_05060"
     CDS             complement(11483..12520)
                     /locus_tag="VHF88_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161175.1"
                     /translation="MEARGSRLGVVVAMLAVAVGTLAASACGDDERGPGVAATLRSAL
                     DRFEERDRQGLCELLSADAKLVIGSAVHLQPTTCERDVGRFFKWLEPYGEQARRPVVT
                     KVTTEGPDRARATVRLADGALVTAPFSREDGTWKLDGLLDATLSRIQARNTPREKGRL
                     LAVSDVPRPAVAGTTSVEHGPGRGAFCPRVDDDRLPAVGGGCLVNLAGKRLTLSAVSP
                     FGRMAFAECDIELDLRVGGDGRGWIATPTIDGRNPCFDAAPCFSKQERGMPWPARIDY
                     RQGKMELAVDVCLDTCVGRFEGRWKLDFVKLGTGWRARSEQGMVGTSGWRFDGVLNTP
                     ARVDVTAQGDI"
     gene            12646..13761
                     /locus_tag="VHF88_05065"
     CDS             12646..13761
                     /locus_tag="VHF88_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161176.1"
                     /translation="MGALIVCCALVAGGCGDDSSAGGVQTADPARTFAPIVHLHPDER
                     LMPADPLWFIARSALWFGDDGGCRNQKISVGKRLAGQQNAYIDWTFVTGLGHGPAYWR
                     EALLADCETDREAYRYYANQRTRPHDDGPGRAPGLRLGEGYYLDLMDWAREGDAVQRT
                     GGRRRVPVPVYYQSRPAEIDGEPGLRLTYWLMFAMNEQPAARGRGDAMTHEGDWERVE
                     VRLRGDDGEYEPVAVRLSTPGERTRELPWSAVRRVVAGAGARTHPVLLAARGSHTLYA
                     RPGRQRRQVELEDGKAVEAVDVAAGPCDACASWPTWQALEEARRQPWYGFGGAWGDRG
                     PSDLTTGPLGPHGDDWPKGDPADDHRRARANRRLRRE"
     gene            13779..>14697
                     /locus_tag="VHF88_05070"
     CDS             13779..>14697
                     /locus_tag="VHF88_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX2161177.1"
                     /translation="MPSLRIAGTVAVLLAGLAPSAQAESSGLGGFVGAAPGGVGGRFA
                     EPSDVAVYLGDRGDPADDKLFVVEALPGNNRVQRLDRHGNFELAWGRDVVRPGARGDT
                     GTGYEVCRRSVSGAAGCKRARRGAERGELSNPTAIAVSQSPGRVYVMDHGNRRVQELD
                     LDGRFVRAWRHGFAGTGNGSIAVSPAPPHHVFVGDPGKRRVLELEPDGRFVRAWRPGR
                     GVRRWPSHVAVDRRGVLYASEGVDNADVLRFDTRSPSRGRVAPLRSGEQLSSGWTMGL
                     EVDPASGALVVARNPFGPMVVDVVHRPGGP"
BASE COUNT         2056 a         5248 c         5202 g         2191 t
ORIGIN      
        1 cgcgccgccg ccctcgcggc ggcgcggagc tggaggggcg atctggagcg catgggcgca
       61 accgtagcga ctggacggtg gagacgggag cggtcccagg gcgcgaggcg cctaccggct
      121 agggcgtgaa ccggacggtc atgacctcgc cgtcctgcat gacgtagtcg cggccctcga
      181 cgcgcaactt gccgcggtcg cgggcggcgg cgtagccgcc aaggtcgacg aggtcgcgcc
      241 agccgatcac ctccgccgcc acgaagccct tctcgatgtc ggtgtggatc ttgccggccg
      301 cgcggcgggc ggaggtgccc gcgcggatcg cccaggcgcg gccctccttg ccctccccgg
      361 cggtgaagaa cgagatcagc ccgagcagct tccacgcctc gcggaccacg gtggccaggc
      421 ccgactccgc gacgccgagg tcggcgcgca tcgcggcggc ctcctcgtcg tcgagctcgc
      481 cgagctcgga ctcgaggcgg gcggacacgg cggcggcgcg ggcgccgagg ccctcggcgt
      541 gggccgccag ctcgggcggt ggtgcgagcg ccccgtccgg gcccgtgtcg gccgcgtcct
      601 cgtcgacgtt cgccaggtag aggacaggct tcgccgtcag cgggctgagg ttgcgcagcg
      661 cgtcgggcgc gtcctcgggg accggcacag tgcgcgccgg ccggccctcc tgcagcgcgg
      721 cgaccagcgc ctcgacccac tccttctcgg ccaccttctc gcgctcgccg gacttcgcct
      781 ccttggcgac cttgtcgagc cgccgctcgg cctgctcgag gtcggcgaac agcagctcgg
      841 tctcgatcgt ctcgacgtcg gcgagcgggt cgacccggcc ctcggggtgg atcacctggt
      901 cgtcgccatg ggcgcgcacc acgtggacca gggcgtcggt ctcgcggatg tttgcgagga
      961 acttgttgcc gaggccctcg cccttgtggg cgccgcggac caggcccgcg atgtcgtgga
     1021 actcgatcgt ctcgtagacg gcgggggtgg ccttgacggt gtcgacgacc gcgtcgagcc
     1081 gctcgtccgg caccggcacc acggccacgt tcggctcgac cgtcgtgaac gggtagttcg
     1141 ccgcctccgc gccagcctgc gtcagcgcgt tgaacagcga cgacttgccg gcgttcggca
     1201 gcccgacgat cccgaccttc acggcgttgt tcccgggacg ctcatcgcgg cgacgaccct
     1261 acctgccgtc cagcagggcg gcgatcgcgg tcccgaacgc cgccccggcc gcgatgtcgg
     1321 tcgggtagtg gacgccgaca tacccgcgcg agaccgccat cgccttggcg agcgcgtaga
     1381 ccggaatcac cgggagaccg tcagcgggcc gggtcagcgc ccaggccgcg gcgaacgagg
     1441 tcgtcgagtg ggccgagggg aacgacagcg acgtcaccgt cgacgacagc gccggcaggc
     1501 cctccaaagc tggccgcggc cgccgcacca cgtacttcag cccgatgttc gcgaggtagg
     1561 ccagcatcac cgtacgcatc gtgcgcaggt acagcggccg gcgctcgccg tcgaccacgg
     1621 cgcccagcag gcacgccccc tgccacagcg cgccatgctc tccagcgcgc gtgaacagca
     1681 gcaccgcccg ctcgacccgc ggcccgtgcc cgcgggtccg cagcgcacgc agcagcgcca
     1741 gctcgaaccg gcgcagcgcc ttcacgctgc ccgcaccacg ccgcgaccgt ccgtcacgcg
     1801 ccggcgcgcc accgccgcgg gcgcctagaa cccgacggcg cggttgcccg tctcgcgacg
     1861 gacgtagacg acctgggcga ggtcgagctg gagcagctcg tcctcgagct cgagcgcatg
     1921 ccagcgcgcg cccgcgtcgt cggccagcgc gtccatcagc ttgcggtagg cgtcctcggc
     1981 ggtgcgcagc gacagcacct ggccgccctg gaagccgatg tcggtacgag ccatggcgtt
     2041 cgccatctct agtgatcctc cgccggttga acgatctcgg tcagcacgcc gccggtcgac
     2101 ttcgggtgag cgaacgcgac gcgactgccc cgaatcccta cccgcggctc ttcgtcgatc
     2161 aaccggatcc ccgccgccac gagccgcttc agctcggcgt cgatgtcgtc ggtccggtag
     2221 gcgacgtggt gcatcccggc gccgttgcgc tcgagaaacc gcgccacgcc cccctccggc
     2281 ccgatcggcg cgatcagctc gacgtgcgcg ccgccgacct ccagcagcac ggcatgaacg
     2341 ccctgctcct ccaccgtctc acggtgctgc tcgcgcatcc cgagctggtc gcggtacagc
     2401 ccgatcgccg cgtcgatgtc ctcgaccgcg atcccgatgt ggtctatctc gccgaacacg
     2461 gggcgggagt ctaatgccct gcgccgcctg ggcggcggcg gaaccggtcc agcgaagggc
     2521 caaaccctgg ctgggaagcc ctctcgttac aggaaatcgc gtgatgtaac ggtttcaact
     2581 cgatggaagg tcggccacgt tgaacgtccg attacagtgg aagactcgct ggcgtgatcg
     2641 gcaccattga ggcgctgggc gtcttccttc tgatgatcct gccggggttc gtctgccttc
     2701 gcgctttcga gtacggacgt ccgccactac gcattcgggg cgctctcggc gagatcggag
     2761 gcgcggtgat gatcagcacc gtcgtgtgga gcggtctgta cctctggcga gggaaagatc
     2821 tcctcccggt cgtcgtgggt gactctggtg agtcggctcg ccagcgcctc gacgcgttcg
     2881 ccgagctcgc cggcctgagc atcctgatcg gcattgccat cgccctactt gcgcgcttag
     2941 gagaccagct ctcacgccgc gccgcacttt tcctcttgaa gcgtcactcg cggaaatccg
     3001 cgcaggcgcg aaggaacaac gcgaacgcaa cgaacgacga cggtacgccg gtgacgaccg
     3061 tggagaagag tggttggctg acgagggtcc gctccggagt cggtcgatgg atgcggcgtc
     3121 gcctccgtgc ccgaacgctg ccggctagcg cttgggatcg cctgatgacc aggctggcca
     3181 accgcggcaa gccggtggtc tgcaaggtgc ggaccagcga cggggcggag attctaggtg
     3241 cgttcgcgtc ggccggatat gccgactggg aggctgacgg tcgcggcctc ctcctagacg
     3301 tcgaggtcgt cgcggacccc gacggcagga tccgccccct cccaatgagt cagggcatct
     3361 tcatccctgg caacgagatc gtgactctca gcgttgtggc gctcccggat gaggcgctaa
     3421 gcttgaagac cgatgagtga ctggcagcca gatatcgatt tcatcgacgt cccggttcct
     3481 gggaagatcg aggagcacgg catctcgatc gatgctccga tgcccgattt ccacatcgcc
     3541 aatccttggg gggtcgcccc gagcgccccg ccggcaaacg gcgccgcgcc ccagccgagc
     3601 gctccctcgg cgactgccga cgacgcctag ccggccggcg agtcccctcc tagccgccgt
     3661 tgagcggcgc cagccgctcc ggcgttgcga tctcgccctc gacctcggcg acgatccggt
     3721 cgatgtcctc gcgctcgagg gcctcgcggg cgagcagctc gcccgccagc gcctcgagca
     3781 ggtcgcggtg ctcggtgatc agggagaggg cgcggcggtg ggcctggtcg gcgatgaact
     3841 gctgctcgtc gtcgatcgcg cggcgggtgg cgtcggacat cgagtagtcg ccggcgttca
     3901 gctcgcgtga catcaggccg gtgcccatgc cgtactgggt gaccatcgcg cgcgagatgt
     3961 cgtggacgga gcgcaggtcg ccggacgcgc cggtggtgat ctggccgaag acgaggtgct
     4021 cggcggtgcg gccgccgagc aggacgacga ggcggtccat cagctcgtcg cgggtcttga
     4081 ggtagcggtc ctcctcgggc aggttgagcg tgtagccgag cgctgtgccg cgcggcacga
     4141 tcgagacgcg gtggaccttc tcggtgctcg ggagcagctc ggcgcagagc gcgtggcccg
     4201 cctcgtggta ggcgacgacg cgcttctcgt ggtcggtgat cacgcggcgc gactgcatcc
     4261 cggcgacaac tcgctcgagc gccgcctgga agtcggtcgt ggcgacccgc tcgcgccggt
     4321 cgcgcgccgc gaagatcgcc gcctcgttgc agatgttggc caggtcggcg ccggtcagcc
     4381 cgctcgtctg ccgcgccacc agctcgaggt cgattccgcc ctcgagcggc ttgtcgcgcg
     4441 tgtgcacggc gaggatccgc ttgcgtccct cgaggtcggg cggcgtgacg aagacctggc
     4501 ggtcgaagcg gccgggccgc agcagcgccg ggtcgagctt gtcgagcagg ttcgacgcgc
     4561 cgatcacgac gagtccgtcc cgcccgccga agccgtcgag ctcgaccagc agctggttga
     4621 gcgtctggtc cttctcgccc gagacgtcgt tgccgcgcgt cgccccgacc gcgtcgagct
     4681 cgtcgatgaa gacgatcgcc ggggcgctct tgcgcgcctc cttgaacagc cgccggatcc
     4741 gggccgcgcc cagccccgcg aacatctcga cgaacgcgct cgcgctctgg gcatagaagc
     4801 gtgcgttcga ctcgtgcgcg accgccttgg cgagcagcgt cttgccggtc cccggcggcc
     4861 cgtgcagcag gatccccttc gggacccgtg cgccgatctg cttgaagcgc tccgggtcgc
     4921 gcaggaactc gacgacctcg cgcagctcgt ccttggcctc ctcgaccccg gcgacgtcat
     4981 cccagccggt cgagtcggcc gactgcggct tgatctcctg cggcttcatc ttgaagctcg
     5041 gcatgaagcg caacatcgcg atcagcacga ccgcgatcag ccccatgaag atgatcggca
     5101 gccagacgat cgcccagttc tcgaactcct cgaagctgaa ggcgagtggt gtgaggacgg
     5161 caagcagacg catcaccggc tatgtcggcg cacttgacgc gcagcattag gatggagggc
     5221 ttccacctcg atctcgtcga cgaggcgcgc ggcggaccgc cgctcgacga tctgaccttc
     5281 ggcagcgaga aggagcggga gctcgagggg tcgttctagc gcagcgtgct ctgcaccagc
     5341 tcggcgaaga accgcgcggc gagcccgaga tcgtcgaccg ggatccgctc gttggcgcca
     5401 tgaacgagcg gcatcgcctc gaagaggtcc atccggcgct gggggaagaa gccgtaggcg
     5461 acgcagtccg ggaaggcggc gcggaaccag tgcgagtcgc tgaaaccggc gagcgcggtg
     5521 gcggtagtag tcgcctcggg gtcctcggac gcaacaaaag accgaatcgt ctccatcagc
     5581 ggcgtgtcga tcggcgagcg gttgccggtg acggtctcgt cgaaggcgag cgagaagccc
     5641 ggcggcggac cggcggccgg cggctcccag ccgagcgcct cgtggacccg gcgacggact
     5701 cggtcttcgc ccacgccggg cgggacgcgg cagtcgacgc gcgcgctcgc ctctgacgga
     5761 atcacgttga tcttctcgga ggcgccgacc atcgtcggcg cgaaggtgat gccgagcagc
     5821 ggctcgagca tgactgcgat ccgcggatcg gtcgcctcga cgtgggccag cgccgcttcg
     5881 aggtcgttgg tcgggatgcc gagcgcctcg aagaacgcat caggctccgg cgagtgctcg
     5941 ggtaccagcc gggcgttgcc gagcgccgat accagcggcg ccagcttcgt cagcgcgttg
     6001 tcgccgattc gtggcagtga ggcgtgcccg gcgcgcccgt gggcggtcag cgtgaagcga
     6061 tagacgccct tctcggcgac gcagaccggg tagacgcggc cgccgtcgta ctcgaacgcc
     6121 gcccccgcgc cctcgttgac gatcaggtcg gcgcgcacct tgtccgggtg ctgcgagcag
     6181 agccattggg ctccgtgggt agcgccggtc tcctcgtccg cggtcagcac cagcttcagc
     6241 tcgccggcct ccgggcgcca cccctcctgc gccagcgcgc aggccaccgc gacctccgcc
     6301 gccacctggc tcttcatgtc gagcgctccg cgaccccaga cgcagccgtc gtcgcgcagc
     6361 tcgcccgacc acggcgagac ctcccactcg gcgggattcg ccagcacggt gtcgacgtgg
     6421 ccgagcaggc acaacgtcgg tccgtcggtc cccgacggcg cgcccagccg ggcgacgagg
     6481 ttcggccgcc ccggtacggc ggcgagcagc tcgacgtcga agccggcgct ctccagcagg
     6541 ccgcgcaggt actcctggca ctcctgctcg gcgcccggcg gattgacggt ctcgaactgg
     6601 atcagccgct gcagcaaccg cgtcgtctgg cgctccagct cctcccgatc aggcaccgaa
     6661 cgagtatatg ccggcgcttg caatgttccc gagcgggaac atccgggaca gttctggccg
     6721 aagcagtccg tatgttcccg atcgggaact ttgcagcatc tcgaccatcg tggaggctca
     6781 atgttcccga gcgggaacat gacaccatgg cgacgacgat cgacacctca gaggatctcg
     6841 gaagggctgt gcgcgcggcg cggctcgagc tcggcctcac ccaggacgac ctcgcccttg
     6901 ccagcgggac cggtcgacgc ttcatcgtca acctcgagag cgggaagtcg accgttcgcc
     6961 tcgacgcggt cctcgccgtc ctcgcggcgc tcggactgcg aatcgagctg cagcgcgccg
     7021 gcccggccga gggggccgat ccagatgcgc gcgcttgacg tctggctcta cggacgtcac
     7081 gccggccgac tcgagcaggt cgatggtcgc atgcgcttca cctacgcgcc cgagtacgtc
     7141 gccgccggcg gagcgccgca ctcctgctcg ctgccgctcg gcgaccacga gcacgaccgc
     7201 gaggccgagg ccttcttcgc caacctcctg cctgagggag aggcgcgcac gatggtcgcg
     7261 cgccgactcg gggtcagcgc cggcaacgac ttcgggctgc tctacgagat cggcggcgac
     7321 tgcgccggcg ccgtcacgct gctgccgccg ggcgcgtccc cggagacgat cgcggagcgt
     7381 gccgagattc gctggctcga tgagccggca ctcgccgccg cgctcgacga gctgccgcgg
     7441 cggccgctgc tcgccgaccc cgacgagggg atccgcctgt cgctcgccgg cgcgcaggac
     7501 aagctgccgg tcgtcgtgag cgacggccgg atcggcatcc cgctcggccg cacgccgagc
     7561 acccacatcg tcaagacgcc gatcgcccgc ttcgacgaca ccgtcgccaa cgaggcgctc
     7621 tgcctcgagc tcgcccgctc gctcgggctc tcggcggctg ccgcggaggt gcgcacggcc
     7681 gacggccgcg agttcctgct cgtcgagcgc tacgaccgcc gccacaccgc cgccggcctt
     7741 gtcgagcgga tccaccagga ggacttctgc caggcgctag cggtcccgcc gcagctgaag
     7801 tacgagtcgg agggcgggcc cgggctggcg gactgcttcg gcgttctacg cgtcgcctcg
     7861 acgcagctcg cagtcgaccg gctcgcgctg ttggacgccg tcacgctgaa cttcctgatc
     7921 ggcaaccacg acgcccacgg caagaacttc tcgctgctgc tcgaggacgg cgccacccgg
     7981 ctggcgccgc tctacgacct cgtgtcgacg gccgtctacg ccggcctcga ccgcaagatg
     8041 gcgatggcgc tcggcggcga gtaccggcct gactacgtcc gccgccgcca cgtcgagcgc
     8101 ttcgccgagc agacccgcct caaccccgcg gccgtccggc gccgcatgct gcgcttggcc
     8161 gcccgtgccc gcgccgcagc tccgcaggtg gccgagcgaa tcgacccgcg gccggtgctc
     8221 gatcgcgtcc tcgagaccgt cgagcagcgc gccggctggc tcgagaccga gctctagccg
     8281 gccgggaaca tcttgcggct cttgaagagc cagcgcaggt cgccgcgcgg ccggaagcgg
     8341 ccgcgcagca tcagccgccc cggatcgacg cggccggcga cgatgtcgac gaagtcctcg
     8401 aaggcgacct ggaagcggac ggtcgggttc tcgaccgtgc cgggggcggc gtgcgtcgtg
     8461 ccgttggcga cgacgacgtg ccagggctcg tggtcgcgga actcccatac gatcgtcccc
     8521 ggctcgcgcg cggcgccgac gtcgagaccg ctcgcgaccg actcgaacag catttgcacg
     8581 tgctcgggat cgcgcgacgg cgggccgatc ttctcgccga ggtagccggc cttcgagagc
     8641 gtcttgccgc ggatcgcccg ctcgcgcggc gtgccgtcga gcggcatcac cggcggaccg
     8701 ggcagctcct cgagtggcat gccggcgctg cgcagcttcg tctcgagcga catcgcgccc
     8761 tgctcgccga ggtcctccat cgtgaagccg aacgtctcga tgtagcgctc gtcccagccc
     8821 ggcgggacga gcacgttgat cgtccacggc atcacctcgc gcagcaggtc ggcgaccgcg
     8881 tgcgggacct ccgggtccat cttgcgcagg tcgttgagca gcttgacgcc gaagccgatg
     8941 tggcgctgct cgtcggccgc gacgttctcc atccccgcgc ggaagcccgg caggatgtcg
     9001 cggtcctcga ggtagctggt gatgaagtgc tggccaggct gggcgagcgt cgcctcgatg
     9061 atcacgtggt agagcgccac gccggccgca agccgcggga tcgacggatc cttgcgcagc
     9121 tcgtcgcaca tctgctcgag gcggccgaag accttcttga agccccagct cagctcaggc
     9181 tggatcgttc gcaggctgtc gccgaccgag ttgccgccga ccccggcgac ctccttcatg
     9241 aagcggttga agaagaccgc gtggcgcgcc tcgtcgacct gctgggtcgt taggaagtac
     9301 ttctgctcct cgagcggagc ggcgtcgacg tagggggaca ggccgtcggc gaccgcatcc
     9361 tcgccccaga agaagaggca gtagttccag taggcggctt tgcggtcgaa ctccgtgaac
     9421 gcgctctgcc actgctcggc gtccttggag aagtcgatct ccgtcgcctt ccaattcccg
     9481 ttctcccagc gccggtagag atcctcgtag gtgatgttgt cgagggggct ctgggtggtt
     9541 ggaagcgtga cggtcatgta gcgaatgtac cgaaccgcat cacgagccgg aattgtttct
     9601 aagtcggcgc taggctcgtc ggctggtatg accgctaccc catggggtga cgccgaccgg
     9661 cttcgctcgc gaaagttgac gccgggaccc ggccacagtc cggaggcggt gctgctcaat
     9721 cagcgcgagc gcctcttcgc cgccaccgtc gcggtcgtgg cggagaaggg ctacgaggcg
     9781 acgcgcgtcg aggacatcct ggtcaaggcg ggcgtctcgc gcaacgcctt ctacaagcag
     9841 ttcgcgaaca agcgcgagtg ctttctcgcc gcgctcgacg agatcgcggc gctcgccaag
     9901 ccatccgtgt tcgacgtctt cgagcagacc gccgggacgt gggacgagaa gctgagggcg
     9961 atgctcgacg ccttcgcggc tgtgatcgtc gcccagccgg cgatggcccg cgtcggctgg
    10021 gtggaggtct atgccgccgg cccggaggcc gtcgagatcg ccgatcgcat cgaccgctcg
    10081 atcgagaaca tcgtctgccg agccctgacg tattcgccgg agcgcgccgg gatgccgcgc
    10141 gagatcgtcg gcgcgatcgt cggcggcgtg cgcaagatcg ttcacacgcg cgtgcgcgag
    10201 gagcggaccc acgagctgcc gggcctgatg ccggagctct tcgcctggat gcagagctac
    10261 cacaccccgc ccgagcggct gcggcgtccc cggcgggtgc cgccggagtt caccgcgctg
    10321 ccgcaggagc cgaccgaccc gcgcgagcgc atcatgcggg cggtcaccga gctcgtcgcc
    10381 gaggagggct atcccgcgat ggcgatcacc gagatcgccg cgcgcgcgtc ggtgtcgctg
    10441 acgaccttct acgcccactt cgacggcaag gaatcggcgt tcctcgcaac gctcgcagac
    10501 gcgcagcggc agctgttcga ggcgacggtc ccgcacttcc tctcgggcgc ggactggccg
    10561 acgtcggtca gtctcggcgc ccgcgccttc ctcggcttcc tgtcgaccaa ccgcgcgatg
    10621 gcgatgctcg gcggattcgg cgtctgggcg accagccccg cggcgctcga gttgcgcgcc
    10681 cagggagtgg cgctcttcga gacgctgctc aacgagggct accgctgcta cccggagacc
    10741 agcccgatcg ccgccgaggg gatcggcgcg agcctcgacg cgctgctatt caagtcgctg
    10801 caatctgccg gcccggcccg gctctacgag ctcggaccga ctgcgacgtt catcgtcctg
    10861 gcgccgttcg tcggggccgg gcgggcgttc gaactggcga atcagacgtc ggcgggagtc
    10921 gcctaggagg attggtctga ccagtttgta acgcttgaca cgaagcggta cgcgctgtta
    10981 cgctgcgccc gtctcggacg cttcggaggg ttcggcgccc gggaccgcag ttggaggggt
    11041 ggggccgcgc aggaggggcg cggccccatt gcgtttcggg cgtcggccta agggcggacg
    11101 accgtgaccg agcccttcat cctgctgtcg agggaggagt ggaactcgta cgtacccggc
    11161 gtatcgaact cgaccgactc ggcctcgcct gtccgcaaca cgtgggtgtc gaagcgctcc
    11221 tgacggtcga gccgcgcccg atcgagctcg aacgtgtcga tcccgcccgt ctcggccgtg
    11281 ttgagctcgg tcgtcgggtt tacgaacgtc acccgtccgc cgaccgggat cctcacgtgc
    11341 gccgggcgat aggcgccgtc gccgaagacg accgtggcct tcgggggggc ggtgagctgc
    11401 gcgggcgtct cagccccggc gcagcccgag gcggcgagcg cgagtccggc gagggcggcg
    11461 gccgcgatgc cgcgcgcgcc cactagatgt ctccctgcgc cgtcacgtcg acgcgcgccg
    11521 gcgtgttcag cacgccgtcg aagcgccagc cgctcgtgcc gaccatcccc tgctccgagc
    11581 gcgcgcgcca gcccgtgcca agcttcacga agtcgagctt ccagcgaccc tcgaaacggc
    11641 cgacgcaggt gtcgaggcag acgtcgacgg cgagctccat cttgccctgg cggtagtcga
    11701 tccgggccgg ccagggcatt ccgcgctcct gcttggagaa gcacggcgcg gcgtcgaagc
    11761 aggggttgcg accgtcgatc gtcggggtgg cgatccatcc gcggccgtcg ccgccaaccc
    11821 ggagatccag ctcgatgtcg cactcggcga aggccatccg gccgaacggg ctgaccgccg
    11881 acagcgtcag ccgcttcccc gcgagattga ccagacagcc gccgccgacc gcggggaggc
    11941 gatcgtcgtc gacccgcggg cagaaggcgc cgcgcccggg gccatgctcg accgaggtcg
    12001 tgcccgcgac ggctggccgg ggcacgtcgc tgacggccag cagccgtccc ttctctcgcg
    12061 gcgtgttgcg cgcttggatc cgcgacagcg tcgcgtcgag cagtccgtcc agcttccagg
    12121 tgccgtcctc tctgctgaac ggcgcggtca ccagcgctcc gtcggccagc ctcaccgtcg
    12181 cccgcgcacg gtcggggccc tccgtcgtca ccttcgtgac gacgggtcgc cgtgcctgct
    12241 cgccgtacgg ctcgagccac ttgaagaagc gtccgacgtc gcgctcgcag gtcgtcggct
    12301 ggaggtggac ggcactgccg atgacgagct tggcgtcggc cgacagcagc tcgcagagcc
    12361 cctgtcggtc gcgctcctcg aatcggtcga gcgcgctcct caacgttgcg gcgacccccg
    12421 ggccgcgctc gtcgtcaccg cacgcgctgg ccgcgagcgt cccgacggcg acggcgagca
    12481 tcgcgacgac cacgccgagc cggctccctc gtgcctccac tgcatccctc cgcatcgctg
    12541 tcgttccctc caaggcgcct gacggcgcag ccgccagcct acccgggaaa cgcctgtggc
    12601 tgtaaggtcg tgtccatgga gggaatttgg aggggtgccc ctttggtggg ggcgttgatc
    12661 gtctgttgtg ccctggtcgc cggcggctgc ggtgacgact cgtctgccgg cggggtccag
    12721 accgccgacc cggcgcggac cttcgcgcca atcgtgcatc tgcatccgga cgagcgcctg
    12781 atgccggccg atccgctctg gttcatagcc cggtccgcgc tctggttcgg cgacgacggg
    12841 ggctgccgga accagaagat ctcggttggc aagcgcctgg ccggccagca gaacgcctac
    12901 atcgactgga ccttcgtcac gggcctcggt cacggcccgg cgtactggcg cgaggcgctg
    12961 ctcgccgact gcgagaccga ccgcgaggcc taccgctact acgcgaacca gcgcacccgc
    13021 ccgcacgacg atggcccggg tcgcgcgccc ggccttcgtc ttggggaggg ctactacctc
    13081 gacctgatgg actgggcgcg cgagggggac gcggtccaga ggacgggagg gcggagaagg
    13141 gtcccggtgc ccgtctacta ccagagccgt cccgcggaga tcgacggcga gcccggcctg
    13201 cgcctcacct attggctgat gttcgcgatg aacgagcagc ccgccgcgcg cggtcgtggc
    13261 gacgcgatga cccacgaggg cgactgggag cgcgtcgagg tccggctgcg cggcgacgac
    13321 ggcgagtacg agccggtcgc ggtgcgcctt tccacccccg gcgagaggac gcgtgagctg
    13381 ccgtggagcg ccgtgcggcg cgtcgtggct ggcgcgggag cgcgcacgca cccggtgctg
    13441 ctcgccgcgc gcggcagcca cacgctctac gcgcgccccg gccgccaacg tcgccaggtc
    13501 gagctggagg acggcaaggc tgtcgaggcg gtcgacgtcg cggccggccc ctgcgatgcc
    13561 tgcgccagct ggccgacctg gcaggccctg gaggaggcgc gtcgccagcc gtggtacggg
    13621 ttcggcggtg cgtggggcga ccgcgggccc agcgatctca ccaccggtcc gctcggccct
    13681 cacggcgacg actggccgaa gggcgacccg gcggacgacc accggcgggc gcgcgccaac
    13741 cgccggctcc gccgcgagta ggcgacgcga ggagagccgt gccgtcgctg cgcatcgccg
    13801 ggacggtagc cgtgctgctt gccgggctcg ccccgtccgc acaggcggag tcgagtgggc
    13861 taggcggatt cgtcggcgcg gcgccgggcg gcgtgggcgg ccggttcgcc gagccgagcg
    13921 acgtcgccgt ctatctcggc gaccgtggcg atcccgccga cgacaagctc ttcgtcgtcg
    13981 aggcactgcc gggcaacaac cgcgtccagc gcctcgaccg gcatggcaac ttcgagctgg
    14041 cgtggggccg ggacgtggtc cggccgggag ctcgcggtga caccgggacg ggctacgagg
    14101 tctgccgacg atcggtgagc ggcgccgcgg gctgcaagcg cgcgcggcgg ggggccgaga
    14161 ggggcgagct ctccaatccc acggcgatcg cggtgagcca gtcgcccggt cgcgtctacg
    14221 tgatggacca cggcaaccgg cgcgtgcagg agctcgacct cgacgggcgc ttcgttcgtg
    14281 cttggcgtca cgggttcgcc ggcacgggga acggctcgat cgcggtcagc ccggccccgc
    14341 cgcaccacgt cttcgttggc gacccgggca aacggcgcgt gctcgagctc gagccggacg
    14401 gccgcttcgt ccgcgcctgg cggccggggc ggggcgtccg ccgctggccg agccacgtgg
    14461 ctgtcgacag gcgaggcgtt ctgtacgcct ccgagggtgt ggacaacgcg gacgtcctac
    14521 gcttcgacac ccggtcgccg agccgcggcc gggtcgcgcc gctccgctcc ggcgagcagt
    14581 tgagcagtgg ctggacgatg gggctggagg tcgatccggc gagcggcgcg ctcgtggtgg
    14641 cgcgcaaccc gttcggtccg atggtggtcg acgtcgtcca ccggccgggg ggccccc
//
LOCUS       DATABV010000119         4310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1724313, whole genome shotgun sequence.
ACCESSION   DATABV010000119 DATABV010000000
VERSION     DATABV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724313"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..665)
                     /locus_tag="VHF88_05075"
     CDS             complement(30..665)
                     /locus_tag="VHF88_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161178.1"
                     /translation="MRTDRETQPAARAAGNGAGTVERRRSDDDVAGIDRERQHEEFGG
                     FNWGASFFGWLVAVGITVLLTALLSAAGAAIGLTELSGDEARSSADTISIVGGALLIA
                     VLVIGYYAGGYVAGRMSRFDGSRQGLGVWIISLVVTVLLAALGAIAGSEYNVLGQLNL
                     PRIPIDEGSLATGAAIALALALIATLIAAIAGGKVGERYHRKVDRVGLHRD"
     gene            complement(662..790)
                     /locus_tag="VHF88_05080"
     CDS             complement(662..790)
                     /locus_tag="VHF88_05080"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEX2161179.1"
                     /translation="MTEKRAVPKERVRMSKDTVTDEETVSDDVRKEQIDVDEGGQR"
     gene            876..1379
                     /locus_tag="VHF88_05085"
                     /pseudo
     CDS             876..1379
                     /locus_tag="VHF88_05085"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
     gene            1439..1651
                     /locus_tag="VHF88_05090"
     CDS             1439..1651
                     /locus_tag="VHF88_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161180.1"
                     /translation="MTATLYTALLLLAQSDGAGRGDNPSEAGGALILVGAVILAILMV
                     GAGVYFVMRSTTRRRGGAKQGPGREG"
     gene            1651..2061
                     /locus_tag="VHF88_05095"
     CDS             1651..2061
                     /locus_tag="VHF88_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161181.1"
                     /translation="MASKVLALAAEPVSGEMLKSAIGGDHAEGAEVLVVAPALNSKLR
                     FWMSDPDAAIERAEAVQEETVERMSEEGIDAAGDTGESDPLLAIQDALTTFAADEIVL
                     FTHPGGDQNWLEEGVVDEIKERFGLPVAHMVVER"
     gene            2125..>4310
                     /locus_tag="VHF88_05100"
     CDS             2125..>4310
                     /locus_tag="VHF88_05100"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HEX2161182.1"
                     /translation="MKFGSFAGGALVAIATLMTFSAPALAHDSPTGSHGLSEAQLRSF
                     ETFVLGADHAADHARERSEDRRSRQPTPTVGHGTWSELTREALATPGGAYEQSQPLQA
                     VLADDPRARDGEWSAPFALPLYAIHAALLPTGKVLWFGPPDPDFKLNTTWAVLWDPIS
                     GELERVDPPQWRDPADGKLKPANIYCAGQAFLASGELLVAGGTLGRDANNKPFGLNKV
                     YTFDPFEEEWTEQPEMAHGRWYPTNVQLPDGRQAIMSGSNEISDQLYNETVEIFTPAA
                     ARGGRGSLTTLVDANGDPAERGTQGQPPTGGLYPHMLGMPSGKTLVAGPYQADTWYLH
                     RPGAGNRLEWSQVDNLNNRRRYANGVIMPPPPGDTSPPTKALLLGGYGPTPGEPSTDQ
                     KSLRTTEYFDDAQPGLGWRHETQMQVGRSNQNTVLLPDGSMVQVGGGVGLDLYGTVPD
                     HHRVELWDPATKQWRLGAAQQEKRAYHSTAVLLPDGRVVSAGDDGNSGALPALDTAEV
                     YSPPYLFKGPRPTISFAPSTVELSDSFDVETPDDIARAALIAPGATTHANDMHQRYVP
                     LAVEQEADGVQLTVPPSANVAPPGYYMLFLVDDAGVPSIAEFVKVVPDRPDGQIRIVK
                     QTTLPGAPESFEFSGDLGSFSLVHGASETQSVPAGLHDVAQAARAGWTLTGLSCTDGS
                     ATYLSQRRALIDVAPGESVTCTFTSTRDPDPDPDPDPDPDPDPDPEP"
BASE COUNT          648 a         1565 c         1517 g          580 t
ORIGIN      
        1 gggggggggc tccccgtgcc gagctgcggt caatcccggt gcaacccgac gcggtcgacc
       61 ttgcggtggt agcgctcacc gaccttgccg cccgcgatcg cggcgatcag cgtcgcgatc
      121 agggcgagcg ccagcgcgat cgccgcgccg gtcgccagcg atccctcgtc gattgggatc
      181 cgcggcaggt tgagctggcc gagcacgttg tactccgagc cggcgatcgc ccccagcgcg
      241 gccagcagga ccgtcaccac gagactgatg atccagaccc cgaggccttg acggctgccg
      301 tcgaagcgcg acatccgccc cgccacgtag ccgccggcgt agtagccgat cacgagtacc
      361 gcgatcagca atgcgccgcc tacgatgctg atcgtgtcgg cgctcgagcg cgcctcgtcg
      421 ccgctcagct ccgtgaggcc gatcgccgcg ccggcggcgc tcagcagtgc ggtcagcagc
      481 acggtgatac cgaccgcaac gagccagccg aagaacgacg cgccccagtt gaagccgccg
      541 aactcctcgt gctggcgctc gcggtcgatc cccgcgacgt cgtcatcgga ccggcgccgc
      601 tcgacggtgc cggcgccgtt gccggcggcg cgcgccgcgg gctgcgtctc tctgtccgtc
      661 ctcatcgctg tcctccctcg tcgacgtcga tctgctcctt gcgcacgtcg tcggagacgg
      721 tctcctcgtc ggtcaccgtg tccttggaca tgcgtacccg ctccttcggg accgcacgct
      781 tctcagtcac gccgtcgact ggttcgacgt gatcgagaac gccgacggca tctggatcac
      841 gctcgccgac gggctcgacg cgcgccgccg ccggcgccgt cgcgctcggc gcgctgcgcg
      901 caagccggcg cagcggcggc gacgtcgccg aggccctcgt cgtgatgcac cagacgctgc
      961 gcgagctgcc cggcccgcgc gccgagatga ccgctgtggc gacgcgctgg gatccgacga
     1021 gccgcgcgct gaacgtcgcc aactgcggcc acgtgccgcc ggtgatcctg cgcggcagcg
     1081 gcgaggtcga gctcgtccac gccccgtcgg ggccgggcct ggggggccgc tcgagcccga
     1141 agccggtcga gagcgtgacg acgctgacgg cgggcgaccg gctcgtgatc gtctccgacg
     1201 gcgtgatcga gtgcggcgac ggcaacgccg gcctcggcat cgagggcatg atccgggccg
     1261 ccctgcgctc agagcgcgag acggcggccg acacggtccg tcagatccac cgcgccgtgc
     1321 tcgcgacgag cgccgagctc gccgacgacg cgaccgcggt ctgcctctct gtggagtagc
     1381 cgccggctac acctcgcggc gcgatttgtg agtcgcgcga cggggtaggc gtgactgtat
     1441 gacggcgacg ctctacacgg ctctcctgct gctcgcccag agcgatggtg ccggccgcgg
     1501 cgacaaccct tcggaggcgg gcggcgcgtt gatcctcgtc ggagcggtga tcctggcgat
     1561 cctgatggtc ggggccggcg tctacttcgt catgcgcagc accacgcgtc gtcgcggcgg
     1621 cgccaagcag gggccgggac gggagggctg atggccagca aggtgctagc gctcgcggcc
     1681 gagccggtct ccggcgagat gctgaagtcg gcgatcggcg gcgatcacgc ggagggcgcc
     1741 gaggtgctcg tcgtcgcgcc cgcgctgaac agcaagctgc gcttctggat gtccgacccg
     1801 gatgcggcga tcgagcgcgc cgaggcggtc caggaggaga ccgtcgagcg gatgagcgag
     1861 gagggcatcg acgccgccgg cgacaccggc gagtcggacc cgctgctcgc gatccaggac
     1921 gccttgacga cgttcgccgc cgacgagatc gtcctgttca cccatccggg tggggaccag
     1981 aactggctcg aggagggcgt ggtcgacgag atcaaggagc gcttcgggct ccccgtcgcc
     2041 cacatggtcg tcgagcgcta ggcgcgcgcc ggttcggaca actggagatt ggcgcgttcg
     2101 cctgaccgct ggtagcttct tcgggtgaag ttcggctcgt tcgcaggagg ggcgcttgtc
     2161 gcgatcgcca ccctcatgac cttcagcgcc cctgcgctgg cgcacgactc tccgacgggg
     2221 agccatgggc tctccgaggc ccagctgcgc tcgttcgaga cgttcgtgct cggcgctgac
     2281 cacgcggccg accacgcacg tgagcgcagc gaggaccgcc gctcgcggca accgacgccg
     2341 accgtcggcc acggaacgtg gagcgagctg actcgcgagg cgctggcgac tcccggcggg
     2401 gcgtacgagc agagccagcc gttacaggcc gtcctcgcgg acgacccgcg cgcccgcgac
     2461 ggcgagtgga gcgctccctt cgccctgccg ctctacgcga tccacgccgc cctgctgccg
     2521 accggcaagg tgctctggtt cggcccgccc gacccggact tcaagctcaa caccacctgg
     2581 gcggtgctgt gggacccgat cagcggcgag ctcgagcggg tcgacccgcc gcagtggagg
     2641 gatccggccg acggcaagct caagcccgcg aacatctact gcgccggaca ggccttcctg
     2701 gcgagcggcg agctgctcgt cgcgggcggg acgctggggc gcgacgcgaa caacaagccg
     2761 ttcgggctca acaaggtcta caccttcgac ccgttcgagg aggagtggac cgagcagccc
     2821 gagatggcgc acgggcgctg gtacccgacc aacgtccagc tgcccgacgg ccgccaggcg
     2881 atcatgagcg gcagcaacga gatcagcgat cagctctaca acgagaccgt cgagatcttc
     2941 acgcctgcgg cggcgcgcgg cggccgggga tccctgacga cgctcgtgga cgcgaacggc
     3001 gacccggccg agcgcggcac gcaggggcag ccgccgaccg gcggcctgta cccgcacatg
     3061 ctcggcatgc cgagcggcaa gacgctggtc gcgggcccgt accaggcgga cacctggtac
     3121 ctgcatcggc ccggggcggg caacaggctc gagtggagcc aggtcgacaa cctgaacaac
     3181 cgccgccgct acgccaacgg ggtgatcatg ccgccaccgc cgggcgacac gtcgccgccg
     3241 accaaggcgc tgctgctcgg cggctacggc cccactccgg gcgagccgtc caccgaccag
     3301 aagagcctgc ggacgaccga gtacttcgac gacgcccagc ccggcctcgg gtggcgccac
     3361 gagacccaga tgcaggtcgg ccgctcgaac cagaacaccg tcctgctgcc cgacggctcg
     3421 atggtccagg tcggaggcgg ggtcggcctc gacctgtatg ggaccgtgcc cgaccaccat
     3481 cgcgtcgagc tctgggaccc cgcgacgaag cagtggcgtc tcggcgccgc gcagcaggag
     3541 aagcgcgcct accactcgac ggcggtcctg ctgcccgacg ggcgcgtcgt gtcggcgggc
     3601 gacgacggga actccggcgc gctgccggca ctcgatacag cggaggtgta ctcgccgcca
     3661 tacctgttca aggggccgcg cccgacgatc tcgttcgctc cctcgacggt cgagctgagc
     3721 gacagcttcg acgtggagac gcccgacgac atcgcccgcg cggcgctgat cgcgcccggc
     3781 gccaccaccc acgccaacga catgcaccag cgttacgtcc cgctcgcggt cgagcaggag
     3841 gccgacgggg tgcagctcac ggtgccgccg agcgcgaacg tcgcgccgcc cggctactac
     3901 atgctcttcc tggtcgacga cgccggcgtt ccgtcgatcg ccgagttcgt gaaggtcgtt
     3961 cccgaccggc ccgacgggca aatcaggatc gtcaagcaga cgacgctgcc gggagctccc
     4021 gagagcttcg agttcagcgg tgacctgggt tcgttctcgc tcgtgcacgg cgccagcgag
     4081 acgcagtcgg tgccggctgg gctccacgac gtggcccagg cggcccgcgc cggctggacg
     4141 ctcacggggc tcagctgcac ggatgggagc gcgacctacc tcagccagcg acgcgcgctg
     4201 atcgatgtgg cgccgggcga gtcggtgacc tgcaccttca cgagcaccag ggatcctgac
     4261 cccgatcctg acccagaccc agatccggac ccagacccag atccggaacc
//
LOCUS       DATABV010000120         9699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1082876, whole genome shotgun sequence.
ACCESSION   DATABV010000120 DATABV010000000
VERSION     DATABV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082876"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VHF88_05105"
     CDS             <1..942
                     /locus_tag="VHF88_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2161183.1"
                     /translation="IVREVMGEDVTAAELGGPDVQSRNGVCHLVAEGDAHAVELARAL
                     VGYLAPRGAEPGAAAPPANRQEPALDPSSVVPADGRKVYDVRAVIEALADGETLEIAP
                     RWARNVVTALGRIDGRAVGFVANQPRRLGGVIDADAAQKAARFVRTCNAFGLPLIVLV
                     DTPGFLPGTRQEAAGVIRHGAKLLYAFSEATVPRFTVVLRKAYGGGYITMNSQGLGAD
                     LVLAWPGAQIGVVGAEQAVGILERREIAAAGDPRAERERRAATYGERHLTAAVAAESG
                     FVDEVVAPAQTRSRLRWALKTIGASERRGVDRGNVPL"
     gene            939..1571
                     /locus_tag="VHF88_05110"
     CDS             939..1571
                     /locus_tag="VHF88_05110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2161184.1"
                     /translation="MNGLRLRRFAALGDSLSAGGEGDPSTPWPERAARMLAGDGGELA
                     FRNFAVAGATSTDVAIWQLQQAVEFRPDLISLICGANDVLLSVRPDPEVFTAVFDGIV
                     DSLRRRLPEASIVTATYPRIGSLLPLRERTSARIEGGIEAINAAIREIAARRGLTCLE
                     WAGHPGVDDPGNFAHDQFHPSAEGHRRAAAAFVEGLSAVLGPCVPGGDAA"
     gene            1568..2068
                     /locus_tag="VHF88_05115"
     CDS             1568..2068
                     /locus_tag="VHF88_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX2161185.1"
                     /translation="MSRALDPSRPGARVVSRGRTVTEADVAQFAALTGDMHPQHTDAV
                     WAAGSRFGERVAHGMLVLSYCVGLVEFDAERVVALRRVANAVFKRPVALGDTIHVESA
                     VESVDRLDGDHSLVACDWRVLNQDGRLVARATVELVWRGQVATASEPSAAVAPPLDED
                     FVPIPL"
     gene            2068..2838
                     /gene="fabI"
                     /locus_tag="VHF88_05120"
     CDS             2068..2838
                     /gene="fabI"
                     /locus_tag="VHF88_05120"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX2161186.1"
                     /translation="MLAGRRILVTGVITHRSIAFAVAEEAQRQGAELLLTSFGRARRL
                     TERAARRLPDPPDVLELDVNSADDLAALAAELDSRWGTLDGVLHAIAHAPADALGGDF
                     LATPPESAAAAFRTSAYSLSALAAALRPLLSAGERGGGIVALDFDASVAWPAYDWMGV
                     AKAALESIARYLARDLGPDGVRVNLVAAGPLQTPAAGGIAGFDELAGGWAGRAPLGWN
                     PADPTPVAAAVCFLLSDLARGITGEILHVDGGAHAIGA"
     gene            2939..3727
                     /locus_tag="VHF88_05125"
     CDS             2939..3727
                     /locus_tag="VHF88_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2161187.1"
                     /translation="MSTDARAETIAVEPELEVRIPSEAEAARDQDEEWCEVRHDGELR
                     RVRFHDYAEVFSIPGLYERLFYEKLECDSPRTVCELLGQALNDGDVAADELKVLDLGA
                     GNGMVGEELAELGAASIVGIDIIEEAAAATERDRPGIYDDYKVLDLTEVSEDDHRDLR
                     KYDFNCLTTGAALGFGDIPPRAFTTAYDLVDDDGWVAFNIKEDFVDAGDSTGFSRLIR
                     GGLEDGALELVADHRYRHRLAAGGEPIHYVAMVARKRGELSLAA"
     gene            complement(3748..4914)
                     /locus_tag="VHF88_05130"
     CDS             complement(3748..4914)
                     /locus_tag="VHF88_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2161188.1"
                     /translation="MGALSHQHDRAFSASLIYMALGVAAAAVMAAIGVDGLHPLENSR
                     LLEWVSEIAIVIALFATGLRLDRPFGTASWAAVWRLLAIAMPLTIGAIALFGVHAMGL
                     PLGAAILLGALLAPTDPVLAGDIGVGPPEEEDEREPSFAITGEAGLNDGLAFPFVFLG
                     LFVLAGGSGWEVEWLAADVLYAVPVGIAIGAAGGYATAALAVRLRDRDLLSPQFDGWM
                     AIPTVLTVYGLAELAGAYGFLAAFAGGVAFRRYERSHDYNQRVHEGVEAVEKFGELSV
                     ILLIGSMLTLDGLAEPGLSGWLLIPVLLLAIRPLSVLVALAGSRLDWRERAFVAWFGV
                     RGVGSLYYAAVAAGAGVLGDDARPVIWTVLACIVVSVVVHGISAAPLDRRLSRS"
     gene            complement(5006..6217)
                     /locus_tag="VHF88_05135"
     CDS             complement(5006..6217)
                     /locus_tag="VHF88_05135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX2161189.1"
                     /translation="MSLTTTGPLRREIEQALPERPFTVDLWDGSTIASTNGDGGPRFT
                     VNSPRALTHLVRAPGQLGLGRAYVSGDIDVDDLMAVMRVVDSWQPPPIDARARGRLLL
                     AAARAIGVTRPPRVPRSELRPRGKLHSRERDERAVRHHYDVGNEFFELFLDDSMTYSC
                     AIFSRGAQTLEEAQETKRELVCTKLGICEGDRVLDVGCGWGAFAIHAASRHGARVVGI
                     TLSAAQAELARKRVADAGLSDRIEIRVMDYRECSGERFDAIASIGMVEHVGENRIDLY
                     ARQLAGLLKPGGQLLNHGIARLRHTDPAPGSFNDRYVFPDGETLQLSRVLLALERAGF
                     VTEHVEGFASDYAETLRHWYENLDRDLPAAERIAGEERLRVWRLYLRAARNAFLNGTI
                     SVYQARATLAG"
     gene            complement(6287..7381)
                     /locus_tag="VHF88_05140"
     CDS             complement(6287..7381)
                     /locus_tag="VHF88_05140"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX2161190.1"
                     /translation="MSPDAGCVLSVYVNLDPSEFATGPARSSALRSVADEASRAVENG
                     AGELSHEVRLRLREDVERIRAYAGSADFDGTHGLAIFSAGGGDMFEVLHLPSPVPNAV
                     VVDGSPHVAPLVGEPDGAWCVVLVNRRNARILSGDLLGLGEEERIADDVPGRHDQGGW
                     SQARYQRHIDEEARRHLERVAEAVSERCRRGAFDHLLVGGPEEGYSEFVELLDNECRE
                     RLCGRVAVDVENTTVAQVAEAALPLMREHHEAQRDELLQALAQGVGAGGRGAAGLGAV
                     LDCLNEQRVETLLLDGGFAATGAECPTCGWLTADEGGECPADGAELKPCRDLVEPAVH
                     RALAQDADVVRLRDRPEMQAHGGIAALLRF"
     gene            7518..9347
                     /gene="typA"
                     /locus_tag="VHF88_05145"
     CDS             7518..9347
                     /gene="typA"
                     /locus_tag="VHF88_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX2161191.1"
                     /translation="MSRREDIRNVAIVAHVDHGKTTLVDAMLWQSGAFRENQDINVRV
                     MDSMDLEREKGITILAKNTAVRYGDVKLNIIDTPGHADFGGEVERGLTMVDGVVLLVD
                     ASEGPLPQTRFVLRKALEARLPTILVVNKVDRPDARIGEVVDEVYELFLDLDADEEQI
                     DFPIVYTNAKDGWASLEEGAEGSDLKPLMDLIVSHIPAPEHEDDRPLQAHVTNLDASP
                     YVGRLALCRVRHGTIRQGQRVAWCRVDGSVEHATVTNLYVTDALDRVDAAEAGPGEII
                     AVAGMPEITIGETLADPDDARPLPTITVDEPSLSVTIGVNTSPLAGQSGDKLTARQIE
                     ARLQRELVGNVSIRVNPTERPDVWEVQGRGELQLAILVETMRREGFELTVGQPQVLLR
                     QVKGRKHEPVERLTIDTPEEYVGAITQLLAGRKGRMENMANHGTGWVRMDYLVPARGL
                     IGFRTEFLTETRGSGVMHHVFESWEPWAGEMRTRPSGSLVADRSGTTASYALANLEER
                     GTLFVGPGEEVYEGMIVGENSRPEDLNVNAVKEKHLTNIRSSTSDVLVRLSPQRELSL
                     DEALEHVREDECVEVTPAIVRMRKAELSATDRVRTARRAAKAG"
     gene            9386..9637
                     /locus_tag="VHF88_05150"
     CDS             9386..9637
                     /locus_tag="VHF88_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2161192.1"
                     /translation="MEYSEISARDLRNHTRDVLRRAERGEPVRITVNRKPVAELRPID
                     DRPMWVAGAAMEKVIASAPADPGLLEDLRPLREQVVDDR"
BASE COUNT         1406 a         3528 c         3499 g         1266 t
ORIGIN      
        1 atcgtccgcg aggtgatggg cgaggacgtc acggccgccg agctcggcgg cccggacgta
       61 cagagccgca acggcgtctg ccacctcgtc gccgagggcg acgcccacgc ggtcgagctg
      121 gcgcgagcgc tggtcggcta cctcgcgcct cgcggcgccg agccgggcgc cgcggcgccg
      181 ccggcgaatc gccaagagcc cgcgctcgac ccgtcgagcg tcgtccccgc agacggccgc
      241 aaggtctacg acgtccgcgc cgtgatcgag gcgctcgccg acggcgagac gctcgagatc
      301 gcaccgcgct gggcgcgcaa cgtcgtcacc gcgctcggca ggatcgacgg ccgcgccgtc
      361 ggcttcgtcg ccaaccagcc gcgccggctc ggcggcgtga tcgacgcgga cgcggcgcag
      421 aaggcggccc gcttcgtccg cacctgcaat gccttcggac tgccgctgat cgtgctggtc
      481 gacacccctg gcttcctgcc cgggacccgc caggaggccg ccggtgtgat ccgtcacggg
      541 gcgaagctgc tctacgcgtt ctcggaggcg acggtgccgc gcttcaccgt cgtgctgcgc
      601 aaggcctacg gcggcggcta catcacgatg aactcgcagg ggctcggggc cgacctcgtg
      661 ctggcctggc cgggcgcgca gatcggcgtc gtcggcgccg agcaggcggt cggcatcctc
      721 gagcggcgcg agatcgctgc ggccggcgac ccgcgggccg agcgcgagcg gcgcgcggcg
      781 acctacggcg agcgccacct gaccgcggcg gtggccgccg agagcggctt cgtcgacgag
      841 gtcgtggcac cggcccagac gcgctcgcgc ctgcgctggg cgctgaagac gatcggcgcg
      901 tcggagcggc gcggcgtcga ccgaggcaac gtgccgctgt gaacggcctg cgcctgcggc
      961 gcttcgcggc gctcggcgac agcctcagcg ccggcggcga gggcgacccg tcgacgccat
     1021 ggcccgagcg tgcggcacgc atgctggccg gcgacggcgg cgagctcgcc ttccgcaact
     1081 tcgccgtcgc cggagccacg agcaccgacg tggcgatctg gcagctccag caggcggtcg
     1141 agttccggcc cgacctgatc tcgctgatct gcggggcgaa cgacgtgctg ctctccgtac
     1201 gccccgaccc cgaggtcttc acggccgtct tcgacgggat cgtcgactcg ctgcggcggc
     1261 ggttgccgga ggcgtcgatc gtcaccgcga cctatccgcg gatcggctcg ctgctgccgc
     1321 tgcgcgagcg caccagcgca cggatcgagg gcggcatcga ggcgatcaac gcggcgatcc
     1381 gcgagatcgc cgcgcggcgc gggctgacct gcctcgaatg ggccggccat ccgggcgtcg
     1441 acgacccggg caacttcgcc cacgaccagt tccacccgtc cgccgagggg caccgccgcg
     1501 cggcggcggc cttcgtcgag ggactcagcg ccgtgctcgg gccgtgcgtg cccggcgggg
     1561 acgcggcatg agccgcgcgc tcgacccgtc acgccccggc gcgcgcgtcg tcagccgcgg
     1621 tcgcacggtc accgaggccg acgtcgccca gttcgcggcg ctgaccggcg acatgcaccc
     1681 ccagcacacc gacgccgtct gggcagccgg cagccgcttc ggcgagcgcg tcgcacacgg
     1741 gatgctcgtg ctgtcctact gcgtcggcct ggtcgagttc gacgcggagc gcgtggtcgc
     1801 gctgcgccgc gtggcgaatg ccgtcttcaa gcgaccggtc gcgctcggcg acacgattca
     1861 cgtcgagtcg gcggtcgagt ccgtcgaccg gctcgacggc gaccacagcc tcgtcgcctg
     1921 cgactggcgg gtgctcaacc aggacggccg cctggtggcg cgtgcgacgg tcgagctggt
     1981 ctggcgcggc caggtcgcga cggcttcaga gccgtcagcc gcggtggcgc cgccgctcga
     2041 cgaggatttc gtgccgatcc cgctgtgatg ctcgccggcc gccggatcct cgtgacgggc
     2101 gtgattacgc accgcagtat cgccttcgcc gtcgccgagg aggcacagcg ccaaggcgcc
     2161 gagctgctgc tgacgagctt cggccgcgcg cggcggctga ccgagcgggc ggcgcggcgg
     2221 cttccggatc cgcccgacgt gctcgagctc gacgtgaaca gcgccgacga cctcgccgca
     2281 ttggccgctg agctcgacag ccgctggggc acgctcgacg gcgtgctgca cgcgatcgcc
     2341 cacgcgcccg ccgacgcgct cggcggcgac ttcctcgcga cgccgccgga gagcgccgcc
     2401 gccgccttcc gcaccagcgc ctactcgctc agtgcactcg ccgcagcgct gcggccgctg
     2461 ctgtccgctg gcgagcgcgg cggcgggatc gtcgcgctcg acttcgacgc gagcgtcgcc
     2521 tggccggcgt acgactggat gggagtagcc aaggccgcgc tcgagtcgat cgcgcgctac
     2581 ctggcgcgcg acctcggccc cgacggcgtg cgcgtcaacc tcgtcgcggc gggcccgctg
     2641 cagacgccgg ccgcgggcgg catcgcgggc ttcgacgagc tcgcgggcgg ctgggccggg
     2701 cgtgcgccgc tcggctggaa tccggccgac ccgaccccgg tagccgccgc ggtctgcttc
     2761 ctgctctcag acctggcgcg cggcatcacc ggcgagatcc tgcacgtcga cggcggcgcg
     2821 cacgccatcg gcgcctaggg cctaccagcg ttttcttggg ccagaccgcc tagactccgc
     2881 tgtcgctcac ttgcctggag ggccttgtga gaggccgaag acccgattcg aggagaccgt
     2941 gagtacggac gcgcgcgcag agacgatcgc cgtcgagccc gagctggagg tgaggatccc
     3001 gagcgaggcc gaggcggctc gtgaccagga cgaggagtgg tgcgaggtcc gtcacgacgg
     3061 cgagctgcgc cgtgtccgct tccacgacta cgccgaggtc ttctcgattc ccgggctcta
     3121 cgagcggctc ttctacgaga agctcgagtg cgactcgccg cggactgtct gcgagctgct
     3181 cggccaggcg ctcaatgacg gcgacgtcgc ggccgacgag ctcaaggtgc tcgacctggg
     3241 agcgggcaac ggcatggtcg gcgaggagct ggccgagctg ggcgccgcct cgatcgtcgg
     3301 catcgacatc atcgaggaag cggcggccgc caccgagcgc gaccggccgg gcatctacga
     3361 cgactacaag gtcctcgacc tcaccgaggt ctccgaggac gaccaccgcg acctgcgcaa
     3421 gtacgacttc aactgcctga cgaccggcgc cgcgctcggc ttcggcgaca tcccgccgag
     3481 ggcgttcacg accgcctacg acctcgtcga cgacgatggc tgggtcgcgt tcaacattaa
     3541 ggaggacttc gtcgacgccg gcgactcgac cggcttctcg cggctgatcc gcggcgggct
     3601 cgaggacggc gcgctggagc tcgtcgccga ccaccgctac cggcaccggc tggcggccgg
     3661 cggcgagccg atccactacg tggcgatggt cgcgcgcaag cgcggcgagc tgtcgctcgc
     3721 cgcgtagcgg tccacacggc ggtccgttca gctccgcgag aggcggcggt cgagcggcgc
     3781 ggcgctgatc ccgtggacca ccaccgacac gacgatgcag gcgagcaccg tccagatcac
     3841 cggacgggcg tcgtcgccga ggacgcccgc gccggcggcg acggcggcgt agtagagcga
     3901 tccgacgccg cgcacgccga accaggcgac gaacgcccgc tcgcgccagt cgaggcggct
     3961 gcccgcgagc gcgacgagca ccgacagcgg ccggatcgcg agcaggagga cggggatcag
     4021 cagccagccg gacagccccg gctcggccag gccgtccagc gtcagcatgc tgccgatcag
     4081 caggatcacg ctcagctcgc cgaacttctc gaccgcctcg acgccctcgt ggacgcgctg
     4141 gttgtagtcg tgcgaccgct cgtagcggcg gaaggcgacg ccgcccgcga aggcggcgag
     4201 gaagccgtag gcgccggcca gctcggcgag gccgtagacg gtcagcacgg tcgggatcgc
     4261 catccagccg tcgaactggg gcgacagcag gtcgcggtcg cgtaggcgga cggccagcgc
     4321 cgcggtcgcg tagccgccgg cggcgccgat cgcgatgccg accgggacgg cgtagaggac
     4381 gtcggcggcc agccactcga cctcccagcc cgaccccccg gccaggacga acagtccgag
     4441 gaagacgaac gggaacgcca ggccgtcgtt gaggccggcc tcgccggtga tcgcgaagct
     4501 cggctcgcgc tcgtcctcct cctcgggcgg cccgacgccg atgtcgcccg ccagaaccgg
     4561 gtctgtgggg gcgagcagcg cgccgagcag gatcgccgct ccgagcggca ggcccatggc
     4621 gtggacaccg aagagcgcga tcgcgccgat cgtcagcggc atcgcgatcg ccagcagccg
     4681 ccagacggcc gcccacgagg cggtgccgaa cggacggtcg agccgcagtc cggtcgcgaa
     4741 gagcgcgatc acgatcgcga tctcgctgac ccactccaac agccgcgagt tctcgagcgg
     4801 gtgcagcccg tcgacgccga tcgccgccat cacggcggcg gccgcgacgc cgagcgccat
     4861 gtagatcagg ctcgccgaga acgcccgatc gtgctggtgc gagagtgccc ccacggccgc
     4921 gaagacagcg agcccgaaga agagcagcgc gagcgcgtgc aggtcgccga aggcgaagcc
     4981 cttgtcggcc gcgagcggga tcgcctcagc cggccagggt cgctcgggcc tggtagaccg
     5041 agatcgtgcc gttgaggaat gcgttgcgcg ccgcccgcag gtagagccgc cagacgcgca
     5101 ggcgctcctc gccggcgatc cgctcggccg ccgggaggtc gcggtcgagg ttctcgtacc
     5161 agtggcgcag cgtctcggcg tagtccgagg cgaagccctc gacgtgctcg gtcacgaagc
     5221 cggcgcgctc gagcgcgagc agcacgcgcg agagctgcag cgtctcgccg tcggggaaga
     5281 cgtagcgatc gttgaacgac ccaggcgcgg ggtcggtgtg gcgcaggcgc gcgatgccgt
     5341 ggttgagcaa ctgcccgccg ggcttcagca ggcccgcgag ctgacgcgcg tagaggtcga
     5401 tcctgttctc gccgacgtgc tcgaccatcc cgatgctggc gatcgcgtcg aagcgctcgc
     5461 cgctgcactc gcggtagtcc atcacgcgga tctcgatccg gtcactcagt ccggcgtcgg
     5521 cgacgcgctt gcgcgccagc tcggcctgcg ccgccgagag cgtgatcccg acgacgcgtg
     5581 cgccgtggcg agaggcggcg tggatcgcga aggcgcccca gccacagccg acgtcgagca
     5641 cccggtcgcc ctcgcagatg ccgagctttg tgcagacgag ctcgcgcttg gtctcctgcg
     5701 cctcctcgag cgtctgcgcc ccgcgcgaga agatcgcgca gctgtaggtc atcgagtcgt
     5761 cgaggaacag ctcgaagaac tcgttgccga cgtcgtagtg gtggcgcacc gcgcgctcgt
     5821 cgcgctcgcg gctgtgcagc ttgccgcgcg gtcgcagctc ggacctcggg acccgcggcg
     5881 gtcgcgtgac gccgatcgcg cgggcggcgg cgagcagcag gcgtccgcgt gccctggcgt
     5941 cgatcggcgg cggctgccag ctgtcgacga cgcgcatcac cgccatcagg tcgtcgacgt
     6001 cgatgtcgcc gcttacatag gcccgaccca ggccgagctg cccgggcgcg cgaaccagat
     6061 gcgtcagggc acgcggggag ttgacggtga agcgcggccc gccgtcgccg ttcgtgctgg
     6121 cgatcgtcga cccgtcccag aggtcgaccg tgaagggccg ctccggcagc gcctgctcga
     6181 tctcgcgccg cagcggacct gtcgtggtca gcgacatccc gtgcacccta accacgtccg
     6241 agcgacacga tcaagcactt gtcgccgaac cccccaaccg ggggtctcag aagcgcagca
     6301 gcgcggcgat gccgccgtgg gcctgcatct ctggccgatc gcgcaggcgc accacgtcgg
     6361 cgtcctgggc gagcgcgcgg tgcaccgccg gctccacgag gtcgcgacac ggcttcagct
     6421 cggctccgtc ggcggggcac tcaccgccct cgtcggcggt cagccagccg caggtcgggc
     6481 actcggcgcc ggtggcggcg aacccgccgt cgagcagcag ggtctcgacg cgctgctcgt
     6541 tgaggcagtc cagcacggcg ccgagcccgg cggcgccgcg accgccggcg ccgactccct
     6601 gcgccagcgc ctggagcagc tcgtcgcgct gcgcttcgtg atgctcgcgc atcagcggca
     6661 gcgccgcctc ggcgacctgc gcgaccgtgg tgttctcgac gtcgaccgcg acgcggccgc
     6721 agagccgctc gcgacactcg ttgtcgagca gctcgacgaa ctcgctgtag ccctcctccg
     6781 gtcccccgac cagcaggtgg tcgaaggccc cgcgccggca gcgctcggag accgcctcgg
     6841 cgacgcgctc gaggtgccgg cgcgcctcct cgtcgatgtg gcgctggtag cgcgcctgcg
     6901 accagccgcc ctggtcgtgg cgtcctggca cgtcgtcggc gatccgctcc tcctcgccga
     6961 gcccgagcag gtcaccgctc aggatgcgcg cgttgcgccg gttgaccagc accacgcacc
     7021 acgctccgtc gggctcgccg accagcggcg cgacgtgggg ggagccgtcg acgacgaccg
     7081 cattcggcac cggagagggc aggtgcagga cctcgaacat gtcgccgccg ccggccgaga
     7141 agatcgcgag cccatgcgtg ccgtcgaagt cggccgagcc ggcgtaggcg cggatccgct
     7201 cgacgtcctc gcgcagccgc agacgcacct cgtgcgagag ctcgccggcc ccgttctcga
     7261 ccgcccgcga cgcctcgtcg gcgaccgatc gcagcgcact cgagcgcgcc gggcccgtgg
     7321 cgaactcgct cgggtcgagg ttgacgtaga cgctgagcac gcagcccgcg tcggggctca
     7381 tctgtgccag ctggcggagt cgggctctac ctggctgtag tacggctggc acgtctagac
     7441 gcttcccctc ggcccgcgga gtcgaaacgc gaccggtccg cccggcccgt ggcgcagcct
     7501 tgtaccctct tgcgcccatg tctaggaggg aggacatacg caacgtcgcc atagtcgcgc
     7561 acgtcgacca tggcaagacc acgctggtcg acgcgatgct ttggcagtcg ggcgctttcc
     7621 gcgagaacca ggacatcaac gtccgggtga tggactcgat ggacctcgag cgcgagaagg
     7681 gcatcacgat cctcgcgaag aacaccgcgg tccgctacgg cgacgtcaag ctgaacatca
     7741 tcgacacgcc gggccacgcg gacttcggcg gcgaggtcga gcgcgggctg acgatggtcg
     7801 acggggtcgt gctgctcgtc gacgcctccg agggaccgct gccgcagacc cgcttcgtgc
     7861 tgcgcaaggc actcgaggcg cggctgccga cgatcctcgt cgtcaacaag gtcgaccgtc
     7921 ccgacgcgcg catcggcgag gtcgtcgacg aggtctacga gctgttcctg gaccttgacg
     7981 cggacgagga gcagatcgac ttcccgatcg tctacacgaa cgccaaggac ggctgggcgt
     8041 cgctcgagga gggcgccgag ggcagcgacc tgaagccgct gatggatctg atcgtcagcc
     8101 acataccggc gcccgagcac gaggacgacc gcccgctgca ggcccatgtg acgaacctcg
     8161 acgcctcgcc ctacgtcggc cggctggcgc tttgccgcgt ccgccacggg acgatccgcc
     8221 aggggcagcg cgtcgcctgg tgccgcgtcg acggcagcgt cgagcacgcg accgtcacga
     8281 acctctacgt caccgacgcg ctcgaccgcg tcgacgcggc cgaggccgga ccgggcgaga
     8341 tcatcgcggt cgcggggatg cccgagataa cgatcggcga gaccctcgcc gatcccgacg
     8401 acgcgcgccc gctgccgacg atcaccgtcg acgagccgtc gctgtcggtg acgatcggcg
     8461 tcaacacctc gccgctcgcc ggtcagagcg gcgacaagct gaccgcgcgc cagatcgagg
     8521 cacggctgca gcgcgagctg gtcggcaacg tctcgatccg cgtcaacccg accgagcgcc
     8581 cggacgtctg ggaggtccag ggccgcggcg agctgcagct ggcgatcctc gtcgagacga
     8641 tgcgccgcga gggctttgag ctgaccgtcg gccagcccca ggtcctgctg cgccaggtca
     8701 aggggcgcaa gcacgagccg gtcgagcggc tgacgatcga cacgcccgag gagtacgtcg
     8761 gcgcgatcac ccagctgctc gccggccgca aggggcggat ggagaacatg gccaaccacg
     8821 gcaccggctg ggtgcggatg gactacctcg taccggcacg cgggctgatc ggcttccgca
     8881 ccgagtttct gacagagacg cgcggcagcg gcgtgatgca ccacgtcttc gagagctggg
     8941 agccgtgggc cggcgagatg cgcacccgcc cgagcgggtc gctcgtcgcg gaccgctccg
     9001 gcacgaccgc gagctacgcg ctggcgaacc tcgaggagcg cggcacgctg ttcgtcggtc
     9061 ccggcgagga ggtctacgag ggcatgatcg tcggcgagaa ctcgcgcccc gaggacctca
     9121 acgtcaacgc cgtcaaggag aagcacctca ccaacatccg ctcctccacg tccgatgtcc
     9181 tggttcggct ctcgccccag cgcgagctct cgctcgacga ggcgctcgag cacgtccgcg
     9241 aggacgagtg cgtcgaggtg acgccagcga tcgtccgcat gcgcaaggcc gaactgagcg
     9301 ccaccgaccg cgtccgcacc gcccgccgcg cggccaaggc cggctgagcc gccagaactg
     9361 cgcacgctgg tgctacattg tgtgcgtgga gtactccgag atatcggcga gggacctgcg
     9421 caaccacact cgcgacgtgc tgcgacgcgc cgagcgtggc gagccggttc ggatcaccgt
     9481 caaccgcaag ccggtcgctg agctgcgccc gatagacgac cgcccgatgt gggtcgccgg
     9541 cgcggcgatg gagaaggtga tcgcctcggc gcctgcggat ccagggctgt tggaggatct
     9601 gcgtccgctg cgcgagcagg tcgtcgacga tcggtgagcc gtgccttgct cgacacgtcg
     9661 gtgttcgtcg ccgaggagca ggggcgtccg ctggcgcgg
//
LOCUS       DATABV010000121        30169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1484330, whole genome shotgun sequence.
ACCESSION   DATABV010000121 DATABV010000000
VERSION     DATABV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 30169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30169
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484330"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VHF88_05155"
     CDS             complement(<1..706)
                     /locus_tag="VHF88_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161193.1"
                     /translation="MRSGTRLAAAWLVIVAVSAGLLGCGDSDSGNVASTPPPEPAQPE
                     DFPSAKGKTLRGLLEDVGSSGPVIAASVSEFTPGKNRFGFALFSRAAAQIADAPVVLY
                     VAPVGGGPVSGPIPARFERMDVKPQFESRTVSEDKDAAKSLYVADIPLPDPGRYELLG
                     LARLDGRIVAATPSGGALTVRRDGPVPDIGERAPAIDTDTVASADGNLESIDTRKPPS
                     SMHDVNFAEVVGRRPTV"
     gene            821..1543
                     /locus_tag="VHF88_05160"
     CDS             821..1543
                     /locus_tag="VHF88_05160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2161194.1"
                     /translation="MAARAALVTGGSSGIGLAIARALGEDGYGVTMSARRPEKLERAA
                     ETLRDDGLDVEAIAANVADEEDIVALVGRHRERFGRLDVLVNNAGVGIGGPIAEHETK
                     KLDMQLAVNLRAVYLTTRECLPMLKEAGAEHGKALVANTASIAGKAGQGWLAAYSATK
                     AGVVGFTQALHKEHAADGVQATALCPGFVDTAMTEWVKDNVPAEQMIQASDIAEAVRY
                     LLRTSPACIVPELQFVRPGEVL"
     gene            complement(1471..1971)
                     /locus_tag="VHF88_05165"
     CDS             complement(1471..1971)
                     /locus_tag="VHF88_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161195.1"
                     /translation="MRPLRRPARELRRAIDEMPLETRRAMLDAIRRNPIIVGAYSSPD
                     GGICPMLAAHRNGGRTDYAEFARAWDRYARAGRRPRPATEGELTALRTMLEASIAEQD
                     VSSAGALARAIADHRRALRSRLRREEHEVEPYEPPDPRELDYSTSPGRTNCSSGTMHA
                     GEVLSR"
     gene            2081..3121
                     /locus_tag="VHF88_05170"
     CDS             2081..3121
                     /locus_tag="VHF88_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2161196.1"
                     /translation="MRLGLHVGYWGLGLTPEQQLEIVKEAESAGFDSVWAAEAYGSDT
                     ATVLAWLASNTERVKIGSAIFQMPGRTPAMTAMTAATLDQLSNGRMLLGIGPSGPQVA
                     EGWHGQPFAKQLQRTREYVEILRKALARERLEYEGELYQLPLPDGPGKALKLMIAPVQ
                     ERMPIYIAAIGPKNTTLTGEIADGWLPTFFSPEHVAEFRTLLEEGAARAGNGKRIDDS
                     FDIAPNVNVCIDDDVDKARDAVRPLLALYVGGMGSRKKNFYNALVQRYGFEDAAKEVQ
                     DLYLDGKKDEAAAALPAELIDRTSLVGPRDRVRERLEVYREAGVGTLVATPTVFDPEA
                     RKRMIRELAEMA"
     gene            3136..4320
                     /locus_tag="VHF88_05175"
     CDS             3136..4320
                     /locus_tag="VHF88_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX2161197.1"
                     /translation="MTAFGDPGHAFPAIALGRALVERGHAVCLQTWRRWAADVEREGI
                     RFAPAPEYSVFPTGRDMTPYEAAVEATRETVRLIESFDPDVVVADILTAVGGLSAGVA
                     ERPFATLIPHVLPTSEPGLPPYSIGARLPRTAFGRRAWGVLQPLLRRGEERGRDELNA
                     ARAKVGLPALDYTHNGISRELALVATFPQLEYPRRKWDGAFRVTGPLLWERPADAVEL
                     PPGDAPLVLIAPSTSQDPQGRMIAAALAGLADEPLRVIATTNRPGMPLPRDVPGNARV
                     VDWLSYSRTMPLCAAVVCHAGHGTVARALSCGVPLVGAPAAGDMAENASRVAWAGCGV
                     SLPRRMVTPRGIRLAVRRLLGEPHFAARASELADWSARHDGGSTAAAALEDFAAARSL
                     DG"
     gene            complement(4333..4755)
                     /locus_tag="VHF88_05180"
     CDS             complement(4333..4755)
                     /locus_tag="VHF88_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2161198.1"
                     /translation="MRAQATTKRELAAGLQAQHGLSMTAYEALYVLSRSEGGRLKRVE
                     LAERLVLTPSGVTRLLEGLEAAGLVERVACDTDLRVTYAQLTDAGVEKLEQASCGHTG
                     SVRSLFEEHLGDDEIEQLAELLGKLPGALDEGEVCPRD"
     gene            4893..5432
                     /locus_tag="VHF88_05185"
     CDS             4893..5432
                     /locus_tag="VHF88_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX2161199.1"
                     /translation="MSTSTLAAPPGTWQLDKVHSSIAFQVPYLAGAFNGTFREFDGTL
                     DVSDDGAALTGTAKVASVDVKDDNLSAHLQSPDFFDAESHPELRFASERIALDGESVR
                     VAGEIEIKGVERAIEATGAAHGPVPNLQGNDSLRLQLTTTVDRTEFGVSWNAELPGGG
                     KALSDDVTILAELYFVKAD"
     gene            5436..6002
                     /locus_tag="VHF88_05190"
     CDS             5436..6002
                     /locus_tag="VHF88_05190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX2161200.1"
                     /translation="MRILAISGSLRRDSHNRALLRAAAEHAPAGVELVLWDGLKAVPP
                     YDEDDDGELAPAAVTALRSGVAGADAVLFATPEYNQSVPGALKNALDWVSRPIATNPL
                     RNKPVAVVGASTGIFGAVWAQAELRKVLGSLGARVLDRELAVGQAAQRIVDGSIADPG
                     VVAELRVVVAELAGAVRARDESIAAAAA"
     gene            6024..6689
                     /locus_tag="VHF88_05195"
     CDS             6024..6689
                     /locus_tag="VHF88_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161201.1"
                     /translation="MATGDSTAALDALTFRVREPAGEPEGLLVLHHGRGTDEHDLFPL
                     LDALDPERRLLGVTPRGPLSLPPGGAHWYAVARVGYPDPETFGAAYASLTAWHDALAA
                     ETGIPPQRTVVGGFSQGAVMSYAVGLGPRRPRPAGILGLSGFIPTVPGWAPDLDRPLP
                     RVAIGHGTLDPVIPVEFGHRARATLEAAGADLLYREYPLPHTIDPAFLQDELVPWLHD
                     TLA"
     gene            complement(6707..6779)
                     /locus_tag="VHF88_05200"
     tRNA            complement(6707..6779)
                     /locus_tag="VHF88_05200"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6744..6746),aa:Asn,seq:gtt)
     gene            complement(6811..7797)
                     /locus_tag="VHF88_05205"
     CDS             complement(6811..7797)
                     /locus_tag="VHF88_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2161202.1"
                     /translation="MTVADDARENGAEEAPVDEVVIEEEGDAYDEFHEGGVVTAGVPA
                     APPAGGTEDAVRLYLQRIGRVPLLTREDEVRLARRIEQNDMAAKNALIEANLRLVVSI
                     AKRYQGRGLTLLDLIQEGNMGLIRGVEKFDWRRGFKFSTYATWWIRQAMTRALADQSR
                     TIRIPVHMVERMNRVHATRRQLSQQLERDPTPAEIAAKLEMPEDKVEHLLKLGQDPVS
                     LETPVGSGEESAELADFIADDAGDRPLEVVARQLRDFDVQAVLDSLPARERAVIELRY
                     GLGDVGPLTLEEIGKEVGLTRERVRQIEVQTLHMLRDSGRAGSLEGTTDDPS"
     gene            complement(7797..9635)
                     /gene="dnaG"
                     /locus_tag="VHF88_05210"
     CDS             complement(7797..9635)
                     /gene="dnaG"
                     /locus_tag="VHF88_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX2161203.1"
                     /translation="MARYTQDSIERLREAVDMVDLVGARTDLRRVGSRWVGLCPFHDE
                     RTPSFSVNADQGLYHCFGCGVGGDAIGFVQETEALPFGEAVELLAERYNVELKRERED
                     PAEEERRRRRGRLLALLERTALFYEKYLWGSDDAAVARDYLAGRGLSEGTLRAYRVGW
                     SPSGWDKLLRGAGRDGFSLDELIAAGLAQPKRSGTGAVDRFRERIMFPLADARGRVLG
                     FGARTLRADEPAKYINTSENEIYHKGRQLFGIDKARVAAAKSRRVVVVEGYTDVLALH
                     QAGVAETVAIMGTALTEHQLAELARAAGAEGVVYLALDADSSGQQAMLRAAALAEQRD
                     VELRVVQMPEGVDPAELVAAEGADAIVGRLDGALSVVEFAVRRVLADAQLGTPEGRDR
                     ALAQARELIAQTPARSARRDHLVQLVADRLDVPADYVVTAVANARRRAPAAPSADAGG
                     DPGPERGGAPGSGGSASAAPAPAAGAGSATLEAERIFLALCLASGPRGRDYLGQLTPD
                     HLSSEVLRRVHAHLIEHFDDPLADLDPEDGELAALAAGIALRADEGEPAEEPLLRMSF
                     LALELRRVDREIRHARQDGDLARQTELASARQQVRRDMDAVMGEVG"
     gene            complement(9650..10645)
                     /locus_tag="VHF88_05215"
     CDS             complement(9650..10645)
                     /locus_tag="VHF88_05215"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX2161204.1"
                     /translation="MRSFAERVASFEEERLAPRATRSYPALRIEPEPDAAHRTPFQRD
                     RDRIVHCKAFRRLKHKTQVFIAPEGDHYRTRLTHTLEACGIARNVARALGLNEDLTEA
                     IGLGHDLGHPPFGHIGERVLDECLQERYGVRFRHNEHSLRVVERLERDGRGLNLTEQV
                     RDGILNHTGPVKAATLEGRIVRVVDRVAYINHDIDDALRAGVIDRADLPADEIATLGE
                     TGSDRIETLVRDLIDRSAAAGDDIVQGEEVGAAMLRLRQFMFDRVYLAADAQRERPRI
                     ERLLRTLFDHYATDLPPAVVPGAAEHERVVDYLAGMTDRFAIRTLADLELPAGYY"
     gene            complement(10629..12173)
                     /locus_tag="VHF88_05220"
     CDS             complement(10629..12173)
                     /locus_tag="VHF88_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2161205.1"
                     /translation="MTATVETVGQLIGGKAVGGTASITVWNPSLGEPIAEVPDGTAAD
                     VNRAVESARAAQRQWWGLAPYERERVLHRIGDLIEERSQRLVQLESVDTGKTVAATVD
                     DVDFAAEVFHFSSAHAARAHGEQIPLSERGTLCYTVLEPIGVVGAITSWNSALVIAAA
                     TVGPALAAGCAVVVKPDQQAPLATLELARIALEAGIPAGCLNVVTGGDETGAALARHP
                     GIDKLSFTGSVETSRSVLRSLAENGTPSGIDTGGKSPNIVFADVDLDTSLDPILRGGL
                     TNGGQECCTGARILVERSILDEFVERAAARVRGMRVGDAADPATEVGPMVSSGHRDRV
                     AGFVERAVAEGARVQAQASAPERGFFFPPTLLEGVSETMEVWREEVFGPVLAIRAFDD
                     DDDDDALEQANGSRYGLAAGVWTADVGRAIRFARELDAGLVWVNSFLDVLSPAAPFGG
                     SKDSGFGSWLGVSGPLEFSRQKTAYVRGVPTGERSDRLSGGPRSSGGLGQSNGGDRTT
                     RREELR"
     gene            complement(12186..12923)
                     /locus_tag="VHF88_05225"
     CDS             complement(12186..12923)
                     /locus_tag="VHF88_05225"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161206.1"
                     /translation="MYRIGLLAPFRELPGEFADVIAEAPEPAAHWQVITAAEDSHRLA
                     ALQAAGDLGTLEAGARRMRRWEPDVVSWACTSGSFVGGRKWAQRQLRAITAASGAPAS
                     STSVAFVEALGALAIDEVAVIAPYPQSAAAAFAAFLAEWGIAVRASVELDAAGPTVSE
                     RLDARDLAPALERVGKELPILLPDTALWGFGLHRELAPRLAVPLLVANQVTLWHAFEI
                     AGVSTDSERFGALRLRAAPGITGDGAY"
     gene            complement(12928..13518)
                     /locus_tag="VHF88_05230"
     CDS             complement(12928..13518)
                     /locus_tag="VHF88_05230"
                     /inference="COORDINATES: protein motif:HMM:NF036518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX2161207.1"
                     /translation="MDRGRLCQVATPQALYEMPANVFVARFMGSPPMNLLIGRIAAEG
                     DGLICHLADSVIDLPEGLVAARPALKAYVGRELIVGVRPDAFALRPPGEEGLAARVYL
                     SELLGSESLVHVETDARAPHGDDGGGSLPPWDAAGDGVGRLSGRFAGDTRGRAPGDAV
                     TLALDPARFELFDLDSGAALARVESHDRSGAVVPRP"
     gene            13559..13735
                     /locus_tag="VHF88_05235"
     CDS             13559..13735
                     /locus_tag="VHF88_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161208.1"
                     /translation="MRDIDGRRAQLLLDPHDLATQLDAQARVEVRQRLVHQEDARIAH
                     ERPPHRNPLPLPAG"
     gene            13889..14080
                     /locus_tag="VHF88_05240"
     CDS             13889..14080
                     /locus_tag="VHF88_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161209.1"
                     /translation="MKDHRHVAIGGHDIADVLAIDQHCSRAELLEAGDRTQRGRLPAA
                     RGADEHHQLAIVDREVDSA"
     gene            complement(14144..14998)
                     /locus_tag="VHF88_05245"
     CDS             complement(14144..14998)
                     /locus_tag="VHF88_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2161210.1"
                     /translation="MTRRGMVASLRQLAVRVGEHGFLLAVALVSLFPLYVMLVASFQR
                     ATSFGGSALLPTLHPTLENYTQVWTELGFDRMFVNSTILSLSSAAIATMLAAGAAYGF
                     TRFRFAGRGMLLVGIVATMAIPPMVVIVPLFVMMSDLGLINQMSSAILVEVGLNLPFA
                     TFLLFTYMRDLPAELFEAAEVDGASRSRQFIEIALPMSGPALVTTMVVCAMYAWNELL
                     IPLVLWQTEQLTTLMVGLALLGPGRSGAQDVPLLMAGVTISVAPLIVAFLIGRRALVR
                     GLVEGGER"
     gene            complement(14995..15876)
                     /locus_tag="VHF88_05250"
     CDS             complement(14995..15876)
                     /locus_tag="VHF88_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2161211.1"
                     /translation="MKAGLAGLRRAAGWPWVLPAVATVGFVLAFPLAKVVQYSLTDRT
                     TFLEGGFVGLSNYSDLLHDELFWRAVKNNLILLLSVPLSIALALAITGILYRGVRGGA
                     IYELLIFLPFLPAIASIAVVFLYLLGNGGPLNELLRGAGLDELTHAWLREPQTAIWGV
                     LGVVLWKRVGFTVLLFMARMASIDRTHFDAAAVDGASWARTYWQVAVPQMRGIIGLAA
                     VFGYIEAFSWTFAYVAILTQGGPDRSTYTLEYLLYKLQFDQQLVGLASAVAVMLLIAA
                     LVVAAYRILRAQREAFA"
     gene            complement(15877..17220)
                     /locus_tag="VHF88_05255"
     CDS             complement(15877..17220)
                     /locus_tag="VHF88_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2161212.1"
                     /translation="MAGSWDRAYTRRQAIGVGAAGVGAFYLAACGGDSGSSGGRDVSV
                     VFDFEYAGKPGSMKRYWETLRDRLERSDVDARLSDLSFVNYANTQARLQSNHAARSGP
                     TLETWYPNWLTYEFIVQDALTPVEDHVRAGATDDWLFAQRIDGKYWGAPFYAEQALLI
                     ANKRHLAKAGVDIGERLESWDAFMDACARIKRSGELPVMIGAADGYACDKWSLASSME
                     YMNSVKQLPLNLLGELSVDEPVVSAWMEYFNQLSRDGYVNDDVVKITEQQSLDRFLDG
                     EGAFAMLYPGIIFDKDPEQFRVVGYWNGPGRYAAPIAVSGNLLLMTSYGENKEAAGRI
                     LDFMQEPEQLQLFSRITGELPCNRNFDPSELGELERTAWRLMTNPGEGKIVTWPRDRM
                     PVVGADVTWELGPRAFAGESPAALRAAYQRRMERYREQNSAQIDEYKKYVDTIEG"
     gene            17639..18322
                     /locus_tag="VHF88_05260"
     CDS             17639..18322
                     /locus_tag="VHF88_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX2161213.1"
                     /translation="MDTAADRAASGIRALIVAGEYESGTRLPSERELAQQLEVSRPAL
                     REGIRRLRSGGVLESRRGSGTYVAEIDAQAVYDVRVRLEPLAAQWAALRRTDAEFTEM
                     RRLVTLMRDRIDEPETFNHSDAQFHNLVARASRSPVLIGTLDRLGELALLTRTAIVNE
                     RRLRVSSLGDVERVCQAIERKHPKRAANAMERHIVNVRADYMAVQEKDASRDGRRRRR
                     SAIKTGDRS"
     gene            18330..18905
                     /locus_tag="VHF88_05265"
     CDS             18330..18905
                     /locus_tag="VHF88_05265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2161214.1"
                     /translation="MSPRHGPEIVLARHGETQWSREMRHTGRTDVPLTDEGRRQAAAL
                     HDRLAGREFAKVLVSPLSRAVETCRLAGLGDRAERRPELVELEYGEAEGLTTAQLRER
                     IPGWTVWTHRTPGAETLEQVGTRLEPLVAELRDTDDDIAIFAHGHILRVLAALWIGLP
                     PQGGARLALSTGTLSTLGWERETQVIRSWNS"
     gene            complement(18958..19674)
                     /gene="recO"
                     /locus_tag="VHF88_05270"
     CDS             complement(18958..19674)
                     /gene="recO"
                     /locus_tag="VHF88_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX2161215.1"
                     /translation="MAGSFKTEAVVLRSIRYGEADRVLHLYSVDRGRIGAMAKGVRRT
                     RSRMGGRLEPLSRVRLVLRQGRGDLSTVSAVDTVSAHPGLREHRSSLERASQACESVL
                     RLLDSGEPNAAAYHLLCNQLALLDGDPTAATRAQSLAFRIKLLLASGFAPELASCASC
                     GEAEHLAGFSPAAGGVVCASCEAGSFPLGAEAHRFLVEALGQPLADAPHASDLALGQA
                     DRAIAETLEHHAHVRLRRVA"
     gene            complement(19710..20549)
                     /gene="mutM"
                     /locus_tag="VHF88_05275"
     CDS             complement(19710..20549)
                     /gene="mutM"
                     /locus_tag="VHF88_05275"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX2161216.1"
                     /translation="MPELPEVETIRRQLAPQLEGRGIARAEVLDARWSEPAAPLEVAD
                     AIEGRRILSVGRRGKYFDLVLEDEVHLVMHLRMTGNLLLVRADDPGEQPYVRVRLRLD
                     DSSSVLFADPRRFGTGLVLLGDDARDEYFSTRLGVEPLSADFTPQALQALARGRRSPV
                     KAFLLAQERIAGVGNIYADEALFRARIHPLRPVGTLRRPQVEALHGAVVESLEAGIDA
                     RGASIDDFRHVDGARGSFQDRFLVHRREGEPCPRCGTAIVKLRAAGRGTYICPRCQRP
                     PRA"
     gene            20588..21526
                     /gene="xerD"
                     /locus_tag="VHF88_05280"
     CDS             20588..21526
                     /gene="xerD"
                     /locus_tag="VHF88_05280"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX2161217.1"
                     /translation="MATVVAPAQSRPFEHLVLDFVAYLEFERGLSRNTLEAYRSDLLQ
                     FGRFLEQRGVTALTAEPVDIADFLASLGGVDGRTASPATINRKQACLRSFYKHLRREE
                     ILSGDPTAKLAGPRKSRRLPQVLTRGEVAKLLDQPQGTEPIALRDRALLELMYACGLR
                     ASEATGLEVTDLDLDELLLRARGKGSKERLVPVGRTAADAVRRYLERGRPQLVGASVE
                     ARLFLNFRGGALTRQGLYKIVQRHARSAGLGDRMSPHTLRHTFATHLLAGGCDLRAVQ
                     EMLGHADVATTQLYTHLSSERLKDVYFSAHPRATAG"
     gene            complement(21523..22617)
                     /locus_tag="VHF88_05285"
     CDS             complement(21523..22617)
                     /locus_tag="VHF88_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX2161218.1"
                     /translation="MSWLLAFAGFALLVILHELGHFAVAKWVGMRVEKFSLFFPPTLA
                     SKKVGETEYAIGLIPAGGYVRITGMSPAEDLPDEVRTRAYHAQPVWKRMAVIAAGPAV
                     NLIVALLLLIVYFGLIGAETEPVDARVDIVEKGYPAAAALQPGDRLLSVDGVRGEPTA
                     LSKQIASHKCPQSPPTDGCRAAAPTTLVIERDGRRIVERLTPIYDADPAAERTRLGFS
                     YVPGPREALPIGDALSVSLDRFWFITEQTVSLPARIFDTERRKEISGIVGSYEVTRQT
                     ILNNLANTVGILAIISLSLAIVNLFPFLPLDGGHLFWAAVEKLRGGKPVPFSVMERAG
                     VVGFMLVIGLFAIGLTNDIGRLSGEGFQVR"
     gene            complement(22614..23765)
                     /gene="dxr"
                     /locus_tag="VHF88_05290"
     CDS             complement(22614..23765)
                     /gene="dxr"
                     /locus_tag="VHF88_05290"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX2161219.1"
                     /translation="MRRIVIAGSTGSIGTQALDVISRSDSLEVVGLAAGSSVDTLLEQ
                     ASAHGVDRIALADPDAAARAAERWTSGRVLGGAEGLVELIVEAECDLVLNSLVGSAGL
                     GPTVAALGEGIDLALANKESLVVGGELVMALAEATGARIVPVDSEHTALHQLIAGLPT
                     GGVDRMTITASGGPFRGRTRSELTEVTAAQALAHPTWQMGGKITIDSATLMNKGLELM
                     EAHHLFGLPYERLDVVVHPQSLVHGLVTLADGAMLAHVGPPDMRIAISYALHGAESVD
                     LPVEPLDLAQVGALSFEPVDGDAFPCLRLAREAAAAGGTAPCVLNAANEIAVHAFLEG
                     ALPFLGIPAVIEQTLAALPERPVRHFSDLYAADAEARERAREACEGALA"
     gene            complement(23776..25449)
                     /locus_tag="VHF88_05295"
     CDS             complement(23776..25449)
                     /locus_tag="VHF88_05295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX2161220.1"
                     /translation="MSVQPHELREVEPPAHQRRPLRAVGRDRGRREAGEREALMRIAA
                     ATADAYRLEDVMEQAAEAALEVTGAASLSISRWEPGREVMRTLINVGQLGPDEQRFPT
                     DEIYPLAEHPLVEKLLRDGEPYRTAVDDPGASPPAVAVLKALGKESDVGVPILLDGEV
                     WGEVWAATAPGAPRFDADDAGFLQAIADRLALVLARGERFARVSRLAYEDPLTGLANR
                     RAIEERLAALLDSDGRQRAPLTLLLADVDGLKDINDAHGHHIGDRALCRVGEALVAAA
                     APEAAALVGRLAGDEFCVLLEGCRIDAANAVARTALELLDDADADLPLSLSFGAAEAG
                     PASTPAELLHAADTAQYAAKRRGGGRLCTSSPAADPLTADLGERRQLSPHRIDQQIEA
                     IAARTMRILDRDAAHRRVLDRLETVVVAFSATLDAAAWAISRWERGSSLIRSVSTADD
                     RDTRLRGLRMGLANEVYRVEDFPNTAELLRRGHGGFVAHVEDDRTDAAERKLLADLGF
                     EAALVAVVAYGSGAYLIEIFADGETAALAPAGIRLQLLLRLAAAPRGAS"
     gene            complement(25534..26307)
                     /locus_tag="VHF88_05300"
     CDS             complement(25534..26307)
                     /locus_tag="VHF88_05300"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX2161221.1"
                     /translation="MLVAVPAALFAIFIVTQGGLVFALGLIVLGVVALRELYGLMRRA
                     RPVDLAGYLAVAGMVLLATYEQRADVLIALVAALPLAFLLAMARPTLANVSWGLAATV
                     LGVVWIGLPLAHAVFLRSLEHGDGLVIDVLVATFVGDTAAYTGGRMWGRRKLAPDISP
                     NKTVEGLVAGIVGATVAFWCAGLYQDWLSGLDALLIGFLVALAAPLGDLLESAVKRDL
                     SVKDSGRVFGAHGGALDRLDAVFLTIVVGFYAALLLGYG"
     gene            complement(26382..27089)
                     /gene="uppS"
                     /locus_tag="VHF88_05305"
     CDS             complement(26382..27089)
                     /gene="uppS"
                     /locus_tag="VHF88_05305"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX2161222.1"
                     /translation="MATGPTDGDGGTGTPRYVAIITDGNGRWAQARGLSPIEGHRAGA
                     DVVKARLRDAAELGVRELTVYSFSTENWKRSPDEVSGLMRMFGERIDGETPELHAEGV
                     RMRFVGRRDGISAELREKMEWAEAATRENTRITLFVAFNYGGRAEIVDAARGFDGTTE
                     EQFRARLYAPEMHDPDLLIRTSGEQRLSNYLLWQCAYSELVFSDELWPDFDRAAFERA
                     LAEYGRRQRRFGARRGS"
     gene            complement(27095..27664)
                     /gene="frr"
                     /locus_tag="VHF88_05310"
     CDS             complement(27095..27664)
                     /gene="frr"
                     /locus_tag="VHF88_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX2161223.1"
                     /translation="MDGDLVKEYLDDASDRMGKTVDSTRHEFGTVRTGRASPHLLDRV
                     DVDYYGAVTPLNQLANVSATEARLLTITPYDKGAIKAIEKAILESDVGLTPSNDGNLI
                     RLSIPELTEERRRELVKVVHGLAEQGRVAVRNVRREVMQELRDMKKDGEIGADDEHRA
                     EAELQKLTDEKVAEIDASLAAKEDEILQV"
     gene            complement(27654..28418)
                     /gene="pyrH"
                     /locus_tag="VHF88_05315"
     CDS             complement(27654..28418)
                     /gene="pyrH"
                     /locus_tag="VHF88_05315"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX2161224.1"
                     /translation="MTGGEGDDGSAPEREAAFGRVLLKLSGEALMGELDYGADPARIQ
                     AIAAQVAHIQRRGVEVAIVVGGGNIYRGMSGAAKGMDRATGDYMGMLATVLNALALQD
                     ALEKQEAVTRVQSAITISEVAEPYIRRRAIRHLERGRIVIFAAGTGNPFFTTDTAAAL
                     RALEIRAEAILMAKNGVEGVYDADPRKVADATFLPAISHREAIERQLGVMDSTALSLC
                     MDNDLPIHVFNMDDERNIDRIVCGDRVGTVVSSDGR"
     gene            complement(28423..29028)
                     /gene="tsf"
                     /locus_tag="VHF88_05320"
     CDS             complement(28423..29028)
                     /gene="tsf"
                     /locus_tag="VHF88_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX2161225.1"
                     /translation="MSTITAGSVKELRERTGAGMMDCKAALVEADGDMDKAIELLRVK
                     GQAKAAKRGERAASEGLVSSYVHHNGKVGVLVEVDCETDFVARNEDFAEFTRDVASHI
                     AAAAPRYVSEDEVPEADCAAELKVFQEQAAEEGKPPQVQEKIAEGRMKKWLEEVVLLN
                     QTHVNADKHDGKTIEELRAAISATTGENVVIRRFARFAIGE"
     gene            complement(29025..30005)
                     /gene="rpsB"
                     /locus_tag="VHF88_05325"
     CDS             complement(29025..30005)
                     /gene="rpsB"
                     /locus_tag="VHF88_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX2161226.1"
                     /translation="MAQVTLRELLEAGVHFGHQTRRWNPKMRRFIFGERSGIHIIDLQ
                     KTERLLANAQDFASSIAARGGTVLFVGTKKQARDTIAEAALASGMPYVNQRWLGGLLT
                     NFQTINKRIKRLHELTEWTETGTLDLLPTRERIAAKKEHDKLTTNLGGVRDMQRVPDA
                     MFVVDLKTEAIAVKEAQRLGIPIIGLVDTNCDPAPVDFVIPGNDDAIRSCKLIIEAIA
                     GTVGEQHAAFRAQEEAARREAEEQARREAEEKARIEAEAAAKREAEEQARKDAATAAA
                     ASGEQPAAPAGQPAEPPPAPAPAEQRSFSPDEDPAPAGQRTFSPEEGAKS"
BASE COUNT         4397 a        10906 c        10541 g         4325 t
ORIGIN      
        1 ggaccgtcgg cctgcggccg acgacctcgg cgaagttgac gtcgtgcatc gacgacggcg
       61 gcttgcgcgt gtcgatgctc tcgaggttcc cgtcagcgct ggcgacggtg tctgtgtcga
      121 tcgccggggc gcgctcgccg atgtccggga ccggcccgtc gcggcgaacg gtcaaggcgc
      181 cgccggaggg agtggcggcg acgatccttc cgtccagccg agccaggccg agcagctcgt
      241 agcgaccggg atcgggcagc ggtatgtcgg cgacgtagag cgacttggcc gcatccttgt
      301 cctcggagac cgtccggctc tcgaactggg gcttgacgtc catccgttcg aagcgggcgg
      361 gaatcggccc gctgaccggc ccgccaccga ccggggcgac gtagagcacg accggcgcgt
      421 ccgcgatctg ggcggcagcg cggctgaaca gcgcgaagcc gaagcggttc ttgccgggcg
      481 tgaactcgga gaccgaggcg gcgatcaccg gcccggacga cccgacgtcc tcgagcaggc
      541 cgcgcagcgt cttgcccttg gcgctcggga agtcctccgg ctgcgccggt tcgggcggcg
      601 gagtgctcgc cacgttgccg ctgtcggagt cgccgcagcc gagcaggccc gcggacaccg
      661 ccacgatcac caaccatgct gccgccaggc gcgttcccga cctcaccgct cagaacatat
      721 gggacaggga cttaccatgg ggccacttcg tgcgcctctt aaccgtcgct tcaaatagcc
      781 tcggcggcgc tctaggcctc taggcagggg agatgcccac atggcagcac gcgcagcgct
      841 agtcaccggc ggttcgagcg ggatcgggct ggcgatcgcc cgcgcgctcg gcgaggacgg
      901 ctacggagtc accatgtccg cacggcggcc cgagaagctc gagcgggccg cagagactct
      961 ccgcgacgat ggcctcgacg tcgaggccat cgcggcgaac gtcgctgacg aggaggacat
     1021 cgtcgcgctg gtcgggcgcc atcgcgagcg cttcggccgg ctggacgtgc tcgtcaacaa
     1081 cgctggggtc gggatcggcg gcccgatcgc cgagcacgag acgaagaagc tcgacatgca
     1141 gctcgccgtc aacctgcgcg ccgtctacct gaccacccgc gagtgcctgc cgatgctgaa
     1201 ggaggcgggc gccgagcacg gcaaggcgct ggtcgcaaac acggcctcga tcgccggcaa
     1261 ggcgggccag ggctggcttg cggcctactc ggcgacgaag gccggcgtcg tcggcttcac
     1321 ccaggcgctg cacaaggagc atgcggccga cggcgtccag gcgaccgcgc tctgcccagg
     1381 gttcgtcgac accgccatga ccgagtgggt caaggacaac gtgccagcgg agcagatgat
     1441 ccaagcctcg gatatcgccg aggcggtccg ctacctgctg agaacctcgc cggcgtgcat
     1501 cgtgcccgag ctgcagttcg tccgccccgg cgaggtgctg tagtcgagct cgcgcgggtc
     1561 gggcggctcg tagggctcga cctcgtgctc ctcgcggcgc agccgtgagc gcagggcgcg
     1621 gcggtggtcg gcgatcgcgc gtgccagcgc gccggcgctg ctgacgtcct gctcggcgat
     1681 gctcgcctcg agcattgtcc gcagcgctgt cagctcgccc tcggtcgccg gtcgcgggcg
     1741 gcggccggcg cgcgcatagc ggtcccaggc gcgagcgaac tcggcgtagt cggtgcggcc
     1801 gccgttgcgg tgagcggcga gcatcgggca gatgccgccg tcgggcgagc tgtaggcacc
     1861 gacgatgatc gggttccttc taatcgcgtc gagcatcgcc cgccgcgtct cgagcggcat
     1921 ctcgtcgatc gctcggcgca gctcccgggc ggggcggcgg agcggtctca tgcgatcggg
     1981 cggtcgatgg gtgccatacg atgccgcgcg gcggccaccc gagggtaggg cgacccgtcg
     2041 gcgacatcaa ggtcgcccct gctgttgaaa ggagacttgc atgcggttgg gcctgcacgt
     2101 tggctactgg ggactcggac tgacgcccga gcagcagctc gagatcgtca aggaggccga
     2161 gtcggcgggc ttcgactcgg tctgggccgc cgaggcctac ggctccgaca ccgcgacggt
     2221 gctcgcctgg ctggcgtcga acactgagcg cgtgaagatc ggctcggcga tcttccagat
     2281 gccgggccgc accccggcga tgaccgcgat gaccgccgcg acgctcgacc agctgtcgaa
     2341 cgggcggatg ctgctgggga tcggcccctc cgggccccag gtcgccgagg gctggcacgg
     2401 ccagccgttc gccaagcagc tgcagcggac gcgcgagtac gtcgaaatcc tgcgcaaggc
     2461 gctcgcgcgc gagcggctcg agtacgaggg cgagctgtac cagctgccgc tgcccgacgg
     2521 gccgggcaag gcgctcaagc tgatgatcgc cccggtccaa gagcggatgc cgatctacat
     2581 cgcggcgatc gggccgaaga acaccacgtt gacgggcgag atagccgacg gctggctgcc
     2641 gacgttcttc tcgcccgagc acgtcgccga gttccgcacg ctgctcgagg agggcgccgc
     2701 gcgcgccggc aacgggaagc ggatcgacga ctcgttcgac atcgcgccga acgtcaacgt
     2761 ctgcatcgac gacgacgtcg acaaggcgcg cgatgcggtg cgcccgctgc tggccctcta
     2821 cgtcggcggc atgggctcgc gcaagaagaa cttctacaac gcgctcgtcc agcgctacgg
     2881 cttcgaggac gcggcgaagg aggtccagga cctctacctc gacggcaaga aggacgaggc
     2941 cgccgcggcg ctgccggccg agctgatcga ccgcacgtcg ctggtcgggc cgcgggaccg
     3001 ggtcagggag cgactcgagg tctaccgcga ggcgggcgtc gggacgctcg tcgcgacgcc
     3061 gacggtcttc gatcccgagg ctcgcaagcg gatgatccgc gagctcgcgg agatggcctg
     3121 agtcgccgct tcctgatcac cgcgttcggc gatccggggc acgccttccc cgcgatcgcg
     3181 ctcggacgag cgctcgtcga acgcggccat gccgtctgcc tccagacgtg gagacgctgg
     3241 gccgcggacg tcgagcgcga ggggatccgg ttcgcgccgg cgccggagta ctcggtcttc
     3301 ccgaccgggc gcgacatgac gccttacgaa gccgcggtcg aggccacgcg cgaaaccgta
     3361 cgactgatcg agtccttcga ccccgacgtc gtagtggcag acatcctgac cgccgtcggc
     3421 ggcctgtcgg ccggggtcgc tgagcgtccg ttcgcgacgc tgatcccgca cgtcctcccc
     3481 acctccgagc ccggcctgcc gccgtactcg atcggcgcac ggctgccgcg gacggcgttc
     3541 ggccgacgcg cctggggcgt gctgcagccg ctgttgcggc gcggcgagga gcgcggtcgc
     3601 gacgagctca acgcggcccg cgccaaggtc ggcctgccag cgctcgacta cacgcacaac
     3661 ggcatctcgc gcgagctcgc gttggtggcg acgtttcccc agctcgaata tccgcggcgc
     3721 aagtgggatg gcgccttccg ggtgaccgga ccgctgctct gggagcgacc ggccgacgcg
     3781 gtcgagctgc cgccgggcga cgcgccgctg gtcctgatcg cgccgagcac gtctcaggac
     3841 ccgcaggggc ggatgatcgc ggccgcgctg gcgggtctgg cggacgagcc gctgcgcgtg
     3901 atcgcgacta ccaaccgacc ggggatgccg ctgccgcgag acgtgccggg aaacgcgcgc
     3961 gtcgtcgact ggctgtcgta ctcgcggacg atgccgcttt gcgctgctgt ggtctgccac
     4021 gcagggcacg gaacggtggc gcgagcgttg tcgtgcggcg tcccgctggt cggagcgccc
     4081 gccgcgggcg acatggccga gaacgcgtcg cgcgttgcgt gggcgggctg cggggtctcg
     4141 ctgccgcgac ggatggtgac gccgcgcggg atcaggttgg cggttcgcag gctccttggc
     4201 gaaccgcact tcgccgcgcg cgcgagtgag ctggcggact ggtcggcacg ccacgacggc
     4261 gggtcgacgg ccgccgcggc gctcgaggac ttcgcggccg cgcgctcgct cgacggctga
     4321 tcggacggac gctcagtcgc gcgggcagac ctcgccctcg tcgagtgcgc ccggcagctt
     4381 gccgagcagc tcggcgagct gctcgatctc gtcatcgccg aggtgctctt cgaagagcga
     4441 tcgcaccgag ccggtatggc cgcaggaggc ctgctcgagc ttctcgacgc cggcgtcggt
     4501 gagctgggcg taggtgacgc ggaggtcggt gtcgcacgcg acacgctcga ccaggccggc
     4561 cgcctcgagc ccctcgagca gccgggtcac gcccgacggc gtcagcacca gccgctcggc
     4621 gagctcgacg cgcttcagcc gcccgccctc ggagcgcgac aggacataga gcgcctcgta
     4681 ggcggtcatc gagaggccgt gctgcgcttg tagcccggcg gcgagctcgc gcttcgtcgt
     4741 cgcctgggca cgcatcagac gggcccacgc ccgcatcgca ggggcggcgt cccccagagg
     4801 tgcttgtggt gtcaatactt gacgattcaa atacttcgcg ctagagttgc ttgcgtagtc
     4861 aactataccc gtcttcctcg gaggtcgcaa ccatgagcac aagcaccctc gcagccccgc
     4921 ccgggacctg gcagctcgac aaggtccatt cctcgatcgc cttccaagtt ccctacctcg
     4981 ccggcgcctt taacggcacc ttccgcgagt tcgacggcac gctcgacgta tccgacgacg
     5041 gagccgccct gaccggcacg gcgaaggtcg cgagcgtcga cgtcaaggac gacaaccttt
     5101 ccgcccacct ccagtcgccc gatttcttcg acgccgagag ccatcccgag ctgcgcttcg
     5161 cctccgagcg gatcgccctc gacggcgaga gcgtccgcgt ggctggcgag atcgagatca
     5221 agggcgtcga gcgtgccatc gaggcaaccg gcgccgccca cggcccggtc cccaacctgc
     5281 agggcaacga cagcctgcgc ctgcaactga cgacgaccgt cgaccggacg gagttcggcg
     5341 tcagctggaa cgcggagctg cccggcggcg gcaaggcact cagcgacgac gtgacgatcc
     5401 tggcggagct gtacttcgtc aaggcggact agcccatgcg gatcctcgcg atatcgggca
     5461 gcctgcggcg cgactcgcac aaccgggcgc tgctccgggc cgcggctgag cacgcgccgg
     5521 ccggcgtcga gctcgtgctc tgggacggcc tcaaggccgt cccgccctac gacgaggacg
     5581 acgatggcga gctggccccg gcggccgtga ccgcgctgcg gtccggggtg gccggagccg
     5641 acgccgtcct gttcgctacg ccggagtaca accaatccgt gcccggcgcg ctgaagaacg
     5701 cgctcgactg ggtgtcgcgg ccgatcgcga ccaacccgct gcgcaacaag ccggtcgcgg
     5761 tagtcggcgc cagtaccggg atcttcggcg cggtctgggc gcaggccgag ctgcgcaagg
     5821 tgctggggtc gctcggcgcc cgcgtgctcg atcgcgagct cgccgtcggg caggccgccc
     5881 agcggatcgt cgatggctcg atcgccgacc cgggggtcgt cgccgagctg cgcgttgtgg
     5941 tcgccgagct ggcgggcgcc gtccgcgcac gcgacgagtc gatcgccgcc gcagcggcct
     6001 gagacgctac gaatagaggg ggaatggcga ccggcgactc gacagcggcc ctcgacgcgc
     6061 tgacctttcg cgtccgcgag cccgccgggg agcctgaggg cctgctcgtc ctccaccacg
     6121 ggcgcgggac cgacgagcac gacctcttcc cgctgctcga cgcgctcgac cccgagcggc
     6181 ggctgctcgg cgtcaccccg cgcggcccgc tgagcctccc gccgggaggc gcccactggt
     6241 atgccgtcgc acgcgtcggc taccccgacc ccgagacctt cggcgcggcc tacgccagcc
     6301 tcaccgcctg gcacgacgcg ctggccgccg agaccggcat cccgccgcag cggacggtcg
     6361 tcggcggttt ctcccagggc gccgtgatga gctacgcggt cgggctcggg ccccgccggc
     6421 ctcgccccgc cggcatcctc ggcctcagcg gcttcatccc gaccgtcccc ggctgggcgc
     6481 ccgacctcga ccgcccactg ccgcgtgtcg cgatcggcca cggaacgctc gacccggtca
     6541 tccccgtcga gttcggccat cgggcgcgcg cgacgctcga ggcggccgga gccgacctcc
     6601 tctaccgcga gtaccccctc ccccacacga tcgaccccgc cttcctccaa gacgagctcg
     6661 tcccctggct acacgacacc ctcgcctagc ggctcctgcg ggcgagctcc gggggtggga
     6721 ctcgaaccca caacctatcg gttaacagcc gatcgctctg ccgattgagc taccccggat
     6781 cgacgcgacg gaccacgata ccgtgagcct tcaggacggg tcgtcggtgg tgccctcgag
     6841 tgagccggcg cggccggagt cgcgcagcat gtggagggtc tggacctcga tctggcggac
     6901 gcgctcgcgg gtcaggccga cctccttgcc gatctcctcg agcgtcagcg gaccgacgtc
     6961 gccgagtccg tagcgaagct cgattacggc gcgctcgcgg gcggggaggc tgtcaagaac
     7021 ggcctggacg tcgaagtcgc gcagctgccg cgcgacgacc tcgagcgggc gatcaccggc
     7081 gtcgtcggcg atgaaatcgg cgagctcggc ggactcctca ccgctgccga cgggcgtctc
     7141 gagcgagacg gggtcctggc cgagcttgag caggtgctcg accttgtcct cgggcatctc
     7201 gagcttggcg gcgatctcgg cgggcgtcgg gtcgcgctcg agctgctggg agagctggcg
     7261 ccgcgtcgcg tggaccctgt tcatccgctc gaccatgtgg acggggatgc ggatcgtgcg
     7321 cgactggtcg gcgagcgcgc gcgtcatcgc ctggcggatc caccaggtgg cataggtcga
     7381 gaacttgaag ccgcgccgcc agtcgaactt ctcgacgccg cggatcaggc ccatgttgcc
     7441 ctcctggatc aggtcgagca gggtcaggcc gcggccctgg tagcgcttgg cgatcgacac
     7501 cacgaggcgc aggttggcct cgatcagcgc gttcttggcg gccatgtcgt tctgctcgat
     7561 ccgccgagcg aggcgtacct cgtcctcgcg cgtcagcagc ggcacgcggc cgatccgctg
     7621 gaggtagagc ctgaccgcgt cctcggtccc gccggcgggc ggcgcggccg gcacgccggc
     7681 ggtgacgaca ccgccctcgt ggaactcgtc gtaggcgtct ccctcctcct cgatcacgac
     7741 ctcgtcgacg ggagcctcct cggccccgtt ctcgcgcgca tcgtcggcga ccgtcatcag
     7801 ccgacctccc ccatcaccgc gtccatgtcg cgccgcacct gctggcgcgc gctcgcgagc
     7861 tcggtttggc gcgcgaggtc gccgtcctgg cgggcgtggc ggatctcgcg gtcgacccgg
     7921 cgcagctcga gcgcgaggaa gctcattcgc agcagcggct cttcggccgg ctcgccctcg
     7981 tcggcgcgca gcgcgatgcc ggcggccagc gcggccagct cgccgtcctc ggggtcgaga
     8041 tcggcgagcg ggtcgtcgaa gtgctcgatc agatgggcgt ggacgcgtcg caggacctcc
     8101 gacgataggt ggtcgggggt tagctgcccg aggtagtcgc gtccgcgcgg gcccgacgcg
     8161 aggcagagcg ccaggaagat ccgctcggcc tcgagggtgg cggacccagc tcccgccgcc
     8221 ggagccggcg cggccgatgc cgagccgccg gatccgggag cgccgccacg ctcgggaccg
     8281 gggtcgcctc ccgcgtccgc agacggcgcc gcgggtgcgc gtcgccgcgc gttggcgacc
     8341 gccgtcacca cgtagtcggc cggcacgtcg aggcggtcgg cgacgagctg gacaaggtgg
     8401 tcgcgccggg cgctgcgcgc cggagtctgg gcgatcagct cacgcgcctg ggcgagcgcg
     8461 cggtcgcgac cctccggcgt gccgagctgt gcatcggcga gcacgcgccg aaccgcgaac
     8521 tcgacgaccg acagggcgcc gtcgagccgt ccgacgatcg cgtcggcccc ctcggcggcg
     8581 acgagctccg caggatcgac accctcgggc atctggacga cgcgcagctc gacgtcgcgc
     8641 tgctcagcga gcgcggccgc gcgcagcatc gcctgctggc cggagctgtc ggcgtcgagc
     8701 gccaggtaga cgacgccctc cgcaccggcg gctcgcgcca gctccgccag ctggtgctcg
     8761 gtcagcgcgg tgcccatgat cgccacggtc tcagccaccc ccgcctggtg cagcgccagc
     8821 acgtcggtat agccctcgac caccacgacg cgccgcgact tggccgccgc cacgcgcgcc
     8881 ttgtcgatgc cgaacagctg tcgtcccttg tggtagatct cgttctcgga ggtgttgatg
     8941 tacttggccg gctcgtccgc gcgcagcgtg cgcgcgccga agcccagtac ccgcccgcgg
     9001 gcgtcggcga gcgggaacat gatccgctcg cggaagcggt cgaccgcgcc ggtaccggag
     9061 cgcttgggct gggcgagccc agcggcgatc agctcgtcga gtgagaagcc gtcgcggccg
     9121 gcgccgcgca gcagcttgtc ccaaccgctc ggcgaccagc cgaccctgta cgcgcgtagc
     9181 gtcccctcgc tcagcccacg gccggcgagg tagtcgcgcg cgacggctgc gtcgtccgaa
     9241 ccccataggt atttctcgta aaacaaggcc gtgcgctcca gcagcgcaag cagccgccca
     9301 cgacggcgcc ggcgctcctc ctcggcgggg tcctcgcgct cgcgcttgag ctccacgttg
     9361 tagcgctccg cgagcagctc caccgcctcg ccgaacggca gcgcctcggt ctcctgtacg
     9421 aacccgatcg catcgccgcc gacgccgcag ccgaagcagt ggtagaggcc ctggtccgcg
     9481 ttcaccgaga acgacggcgt gcgctcgtcg tggaacgggc agagcccgac ccagcgcgag
     9541 ccaacgcggc gcaggtcggt gcgcgcgccg acgagatcga ccatgtcgac cgcctcgcgc
     9601 agccgctcga tcgagtcctg ggtgtagcgc gccatctccg atcgcccgat cagtagtaac
     9661 cagcggggag ctcaaggtct gcgagcgtgc ggatcgcgaa gcggtcggtc atccccgcga
     9721 ggtagtcgac gacgcgctcg tgctcggccg ccccgggcac cacggcgggc ggcaggtcgg
     9781 tcgcgtagtg gtcgaagagc gtgcgcagca gccgctcgat ccgcggccgc tcacgttgcg
     9841 catcggcggc gaggtagacg cggtcgaaca tgaactggcg caggcgcagc atcgctgcac
     9901 cgacttcctc gccctggacg atgtcgtcgc cggccgcggc cgagcggtcg atcaggtcgc
     9961 gcacgagcgt ctcgatgcgg tcggagccgg tctccccgag cgtcgcgatc tcgtctgcgg
    10021 ggagatcggc acggtcgatc acgcccgcgc gcaacgcgtc gtcgatgtcg tggttgatgt
    10081 aggcgacgcg gtcgacgaca cggacgatcc ggccctcgag cgtcgcggcc ttcaccggcc
    10141 cggtgtggtt caggatgccg tcgcggacct gctcggtcag gttgaggccg cggccgtcgc
    10201 gctcgagccg ttcgacgacg cgcagcgagt gctcgttgtg gcggaagcga acgccgtagc
    10261 gctcctgcag gcactcgtcg agcacccgct cgccgatgtg cccgaagggc gggtggccga
    10321 ggtcgtggcc gagcccgatc gcctcggtca ggtcctcgtt gagcccgagc gcacgggcga
    10381 cgttgcgggc gatgccgcac gcctcgagcg tgtgggtcag ccgcgtccgg tagtggtcgc
    10441 cctcgggcgc gatgaagacc tgcgtcttgt gctttagccg tcggaacgcc ttgcagtgga
    10501 cgatccggtc gcgatcgcgc tggaacggcg tgcggtgcgc tgcgtcgggc tcgggctcga
    10561 tccgcagcgc cggatacgag cgggtcgccc gcggcgccag ccgctcctcc tcgaagctcg
    10621 cgactcgctc agcgaagctc ctcacgtcgc gtcgtacgat caccaccgtt cgattgtccc
    10681 aggccgccgg aggaacgcgg gccgcccgaa agtcgatccg accgctcgcc ggtcgggacg
    10741 ccgcggacat aggcggtctt ctggcgcgag aactcgagcg ggccggacac tcccagccag
    10801 ctgccgaagc cgctgtcctt actgccgccg aagggagccg ccggactgag cacgtcgagg
    10861 aagctgttga cccagaccag tcccgcgtcg agctcgcggg cgaagcggat cgcccggccg
    10921 acgtcggcgg tccagacgcc ggccgccagc ccgtagcggg aaccattcgc ctgctcgagc
    10981 gcatcgtcgt cgtcgtcgtc gtcgaaggcg cggatcgcga ggaccgggcc gaagacctcc
    11041 tcgcgccaga cctccatcgt ctcgctgacg ccctcgagca gcgtcggcgg gaagaagaag
    11101 ccacgctcgg gcgcggaggc ctgcgcttgg acgcgagctc cctcggcgac cgcgcgctcg
    11161 acgaagccgg ccacgcgatc gcggtgcccg gagctgacca tcggaccgac ctccgtcgcc
    11221 gggtcggcgg catctccgac gcgcataccg cgaacccgcg ctgcagcacg ctcgacgaac
    11281 tcgtcgagaa tcgatcgctc gaccaggatc cgcgcgcccg tgcagcactc ctggccgccg
    11341 ttggtcagcc caccgcgcaa tatcggatcg agcgacgtgt cgaggtcgac gtcggcgaag
    11401 acgatattcg gactcttgcc gccggtgtcg atcccgctcg gcgtcccgtt ctcggccagt
    11461 gaccgcagga ccgaccggct ggtctcgacc gatccggtga agctcagctt gtcgatgccg
    11521 gggtggcggg cgagcgcggc accggtctcg tcgccaccag tcacgacgtt gaggcatcca
    11581 gccgggatgc ccgcctcgag ggcgatccgc gccagctcga gggtcgcgag cggagcctgc
    11641 tgatcgggct tgacgacgac ggcgcagccc gccgcgagcg cgggccccac ggtcgcggcc
    11701 gcgatcacca gcgccgagtt ccacgaggtg atggcgccta cgacgccgat cggctcgagg
    11761 accgtgtagc agagcgtccc gcgttcggag agcgggatct gctcgccatg cgcccgcgcc
    11821 gcgtgcgccg agctgaagtg aaagacctcg gccgcgaagt cgacgtcgtc gacggtggcg
    11881 gcgaccgtct tgcccgtgtc gacgctctcg agctggacga gacgctggct gcgctcctcg
    11941 atcagatcgc cgatccggtg caggacgcgc tcacgctcgt acggcgcgag cccccaccac
    12001 tggcgctgcg cggcacgcgc cgactccacg gcccgattca cgtccgccgc cgtgccgtcc
    12061 gggacctcgg cgatcggctc cccgaggctg gggttccaga cggtgatcga cgcggtgccg
    12121 cccacggcct tccctccgat gagctgacca acggtctcga cggtggcggt catcgctgtc
    12181 tcctcctagt aggcgccgtc accagtgata ccgggcgcgg cgcgcaggcg caacgcgccg
    12241 aagcgttccg agtccgtcga cacccccgcg atctcgaagg cgtgccagag cgtcacctgg
    12301 ttggccacca gcagcggtac ggccaaccgc ggcgccagct cgcgatgaag cccgaagccc
    12361 caaagggcgg tgtcgggcag cagtatcggg agctccttgc cgacccgctc gagcgctgga
    12421 gcgaggtccc gcgcgtcgag ccgctcggag acggtcgggc cggccgcgtc gagctccaca
    12481 gacgcccgga cggcgatacc ccactcggcc aggaacgcgg cgaaggccgc cgcggccgac
    12541 tgtggatagg gcgcgatcac ggcgacctcg tcgatggcga gcgcgccgag cgcctcgacg
    12601 aaggccaccg acgtgctgct ggcgggagcg ccagacgcgg ccgtgatcgc ccgcagctgg
    12661 cgctgcgccc acttgcgccc gcctacgaac gagccgctgg tgcacgccca cgacaccacg
    12721 tcgggctccc accgccgcat cctccgtgcg cccgcctcca gcgtgccgag gtcgccggcg
    12781 gcttgcagcg ccgcgagccg gtgactgtcc tcggcggccg tgatcacctg ccagtgcgcc
    12841 gccggctcgg gcgcctcggc gatcacgtca gcgaactcac cgggcaactc gcggaacggc
    12901 gccagcaggc cgatgcggta cacgcgacta cggcctcggc acgaccgcgc ccgagcggtc
    12961 gtgcgactcg acgcgcgcga gcgccgcgcc gctgtcgagg tcgaagagct cgaagcgcgc
    13021 ggggtccagc gcgagcgtga cggcatcgcc aggcgccctt ccccgcgtat cgcccgcgaa
    13081 ccgtccgctg aggcgaccga cgccatcgcc ggcggcgtcc cacggcggca gcgaaccgcc
    13141 gccgtcgtcg ccgtgtgggg cgcgcgcgtc ggtctcgacg tggaccagcg actccgaacc
    13201 gagcagctcg ctgagataga cccgggcggc gagtccctcc tcgcccggcg gacggagcgc
    13261 gaacgcgtcg ggtctcacgc cgacgattag ctcgcggccg acgtaggcct tgagggcggg
    13321 ccgtgccgcg accagtccct ccggcagatc gatgacgctg tcggccaggt gacagatgag
    13381 cccgtcgccc tcggctgcga tgcggccaat gagcaggttc atcggcgggc tgcccatgaa
    13441 gcgcgcgacg aacacgttcg cgggcatctc gtagagcgcc tgcggcgtcg ccacctgaca
    13501 cagtcgtccg cggtccatca ccgcgacccc gacccatggt catcgcctcg acctgatcat
    13561 gcgtgacata gacggtcgtc gtgcccagct cctgctggat ccgcacgatc tcgccacgca
    13621 gctggacgcg caggcgcgcg tcgaggttcg acagcggctc gtccatcagg aagacgcgcg
    13681 gatcgcgcac gagcgcccgc cccatcgcaa cccgctgccg ctgcccgccg gatagctcac
    13741 gcgggcgccg atcgagcagc gacgcgatgt cgagggccgc cgcgatccgc tcgactcgct
    13801 cgcgctgctc ggcgcgcggc acgccacgag cgcgcagccc gaacgcgatg ttgcgcgcca
    13861 cgctcatgtg ggggtagagc ccgtagcttt gaaagaccat cgccacgtcg cgatcggcgg
    13921 ccacgacatc gccgacgtcc tcgccatcga tcagcactgc tcccgagctg agctgctcga
    13981 ggccggcgac cgcacgcaac gcggtcgtct tcccgcagcc cgaggagccg acgagcacca
    14041 tcagctcgcc atcgtcgacc gcgaggtcga ttccgcgtag cgcctcgacg ccgttgcgaa
    14101 aggcctttcg cgcgtccctg agctcgaccg aggccacgcc tgcctaccgc tcgccgccct
    14161 cgaccagacc acgcacgagc gcccgcctcc caatcaggaa agccacgatc aagggggcga
    14221 ccgagatcgt cacgccggcc atcaacagcg gcacgtcctg cgcgccgctg cgacccggac
    14281 cgagcagcgc gagcccgacc atcagcgtgg tcagctgctc ggtctgccac aggaccaagg
    14341 ggatcagcaa ctcgttccag gcgtacatcg cgcagacgac catcgtcgtc acgagcgccg
    14401 ggcccgacat cggtagtgct atctcgatga actgccgcga gcgcgacgcg ccgtcgacct
    14461 cagcggcctc gaacagctcc gcagggagat ccctcatata cgtgaacagc aggaaggtcg
    14521 caaacgggag gttcaagccg acctcgacga ggatcgcaga cgacatctgg ttgatcagac
    14581 cgagatcgct catcatcacg aacagcggga cgatgacgac catcggcggg atcgccatcg
    14641 tcgccacgat ccccacgagc agcatgcccc gcccggcgaa gcggaaccgc gtgaagccgt
    14701 aggctgcgcc cgcggcgagc atcgtcgcga tcgccgccga cgagaggctg aggatcgtgc
    14761 tgttgacgaa catccggtcg aagcccagct cggtccagac ctgggtgtag ttctccaaag
    14821 tcgggtgcag cgtcggcagc agcgcagacc ctccgaagct cgtcgctcgc tgaaacgacg
    14881 ccaccagcat cacgtagagc gggaacagcg acaccaacgc gaccgcgagc aggaacccgt
    14941 gctcgccgac gcgcacggcg agctgccgca gactcgcgac catgccgcgt cgggtcatgc
    15001 gaacgcctca cgctgcgcgc gcaggatccg ataggccgcc acgacgagcg ccgctatcag
    15061 cagcatcacc gcgaccgccg acgccagccc gaccagctgc tggtcgaact ggagcttgta
    15121 gagcaggtac tcgagcgtgt aggtgctgcg atccgggccg ccctgtgtga ggatcgcgac
    15181 gtaggcgaag gtccaggaaa aggcctcgat atagccgaag accgctgcca ggccgatgat
    15241 gccgcgcatc tgcggcaccg ccacctgcca gtaggtgcgt gcccagctgg ccccgtcgac
    15301 ggccgccgca tcaaagtgtg tgcgatcgat gctcgccatg cgcgccatga acagcagcac
    15361 ggtgaagccg acgcgcttcc acaggactac ccccaggacg ccccagatcg cggtctgcgg
    15421 ctcgcgcaac caggcgtgcg tgagctcgtc gagccccgcc ccgcgcagca gctcgttgag
    15481 cgggcccccg tttccgagca ggtagaggaa gacgacggcg atcgacgcga tcgccggcag
    15541 gaagggaagg aagatcagca gctcgtagat cgcaccgccg cgcacgccgc ggtagaggat
    15601 tccggtgatg gcgagggcca gcgcgatgct cagcgggacg ctcagcagga ggatcaggtt
    15661 gttcttgacc gcccgccaga acagctcgtc atggagcagg tcggagtagt tgctcagtcc
    15721 gacgaagccg ccctcgagga aggtcgtacg gtcggtgagg ctgtactgga cgaccttcgc
    15781 cagggggaac gcgagcacga acccgaccgt cgcgacggct ggaagcaccc acggccagcc
    15841 cgctgcgcgc cgcagccccg cgagccccgc cttcacctac ccctcgatcg tgtcgacgta
    15901 cttcttgtac tcgtcgatct gggcactgtt ctgctcacgg tagcgctcca tccggcgctg
    15961 gtaggcggct cgcagcgcag ccgggctctc gcccgcgaac gctctcggac ccagctccca
    16021 ggtgacgtcc gccccgacga ccggcatccg atcgcgcggc caagtgacga tcttgccctc
    16081 gcccggattg gtcatcaacc gccacgccgt ccgctcgagc tcgccgagct cgctcggatc
    16141 gaagttgcgg ttgcacggca gctcgccggt gatccggctg aagagctgca gctgctcggg
    16201 ctcctgcatg aagtcgagga tccggccagc ggcctctttg ttctcgccgt agctggtcat
    16261 cagcagcagg tttcccgaca ccgcgatcgg cgccgcgtag cgcccggggc cgttccagta
    16321 gccgacgacc ctgaactgct cgggatcctt gtcgaagatg atccccgggt acagcatcgc
    16381 gaaggccccc tcgccgtcga ggaagcggtc gagcgactgc tgctctgtga tcttgaccac
    16441 atcgtcgttg acgtatccgt cgcggctgag ctggttgaag tactccatcc acgccgagac
    16501 gaccggctcg tcgaccgaca gctcgccgag caggttgaga ggaagctgct tgaccgagtt
    16561 catgtactcc atgctcgacg cgagcgacca cttgtcgcag gcgtagccgt cggcggcgcc
    16621 gatcatcacc ggaagctcgc cggaccgctt gattcgagcg caggcgtcca tgaacgcgtc
    16681 ccaggactcg agacgctcgc cgatgtcgac gccggccttc gcgaggtgtc gcttattggc
    16741 gatcagcagc gcctgctccg cgtagaacgg cgcgccccag tacttcccat cgatccgctg
    16801 ggcgaacagc cagtcgtcgg tggcgccggc gcgaacgtgg tcctctacgg gtgtgagggc
    16861 gtcctggacg atgaactcgt aggtgagcca gttcgggtac caggtctcga gcgtcggccc
    16921 gctgcgcgcg gcgtggttgc tctggaggcg cgcctgggtg ttggcgtagt tgacgaacga
    16981 caggtcgctc agccgggcat cgacgtcgga ccgctcgagc cggtctctga gcgtctccca
    17041 ataccgcttc atgctgcctg gcttaccggc gtactcgaag tcgaagacga cggacacgtc
    17101 gcgcccgccg ctcgagcccg agtccccgcc acaggcggcg aggtagaacg cgcccactcc
    17161 cgccgcaccg acgccgatcg cctgtcggcg cgtgtaggca cgatcccagc ttccagccat
    17221 gtctcctcct tgtccctcag cgatgcggcc ggggctacct agcccaacct ccgtcgccga
    17281 ccatacacgc gattaccgac tcgttcaggc ttgcattcgt ccgggtcggc tgtaacatgg
    17341 tctggccaat gtggtcaggc caattctttc cggctgtgca cttcgacgtt caagacttcg
    17401 atccagatcg tgcctcgcgg cacgggatcg tccaaacacc ggctgcggtc ggcgcaggca
    17461 tgcatcacgg accgaaccct ccccgtgagc ccgccggcgc cgactcgtag agcagcgctc
    17521 gccggcggcg gttcgcccat ccctccgggc gccgccgccg gtgccggtcc tttcagccac
    17581 tcgactgtga cggttgagcc gttctcacta tccgtgtgac ccacgttaga ctcggcgagt
    17641 ggataccgca gcagatcgtg cagccagcgg aatccgggcg ctgatcgtcg ccggagagta
    17701 cgagtccggc acgcgactgc cgtccgagcg tgagctggcg cagcagctcg aggtctcgcg
    17761 cccggcgctg cgcgagggga ttcgccgcct gcgcagcggt ggcgtgctcg agtcgcgacg
    17821 gggatcgggc acctacgtcg cagagatcga cgcccaagcg gtgtacgacg ttcgcgtgcg
    17881 actcgagccg ttggcggctc aatgggccgc actgcgtagg accgacgcgg agttcaccga
    17941 gatgcgccgc ctggtcactc ttatgcgtga caggatcgac gagcccgaga ccttcaacca
    18001 ttcggacgcg cagttccaca acctggtcgc tcgcgcgagc cgcagccccg tgctgatcgg
    18061 cacgctcgac cggcttggcg agctggcgct gctgacgcga accgcgatcg tcaacgagag
    18121 gcgtctgcgc gtctcctcgc tcggcgacgt cgagcgcgtc tgccaggcga tcgagcgcaa
    18181 gcacccgaag cgcgccgcga atgcgatgga gcgccacatc gtcaacgttc gcgccgacta
    18241 catggcggtt caggagaagg acgcgagccg cgacggacgg cggcgccgcc gtagcgcgat
    18301 caagaccggc gataggtcgt agtcgcacgt tgtcgcctcg ccatggtccc gagatcgtcc
    18361 tcgctcgcca cggcgagacg cagtggagcc gcgagatgcg ccacaccgga cgcaccgacg
    18421 tgccgctgac cgacgagggt cggcgtcagg cggcggccct gcacgaccgc ctcgccggtc
    18481 gcgagttcgc caaggtcctg gtcagcccgc tgtcgcgcgc cgtcgagacc tgccggctcg
    18541 cgggcctcgg cgatcgggcc gagcggcgcc ccgagctcgt cgagctcgag tacggcgagg
    18601 ccgagggcct cacgaccgcc cagctgcgcg agcgcatccc cggctggacg gtctggacgc
    18661 accgcacgcc cggagccgag accctcgagc aggtcggcac tcgcctcgag ccactggtcg
    18721 ccgagctgcg cgacaccgat gacgacatcg cgatcttcgc ccacggccac atcctgcgcg
    18781 tcctcgccgc cctctggatc gggctaccgc cccagggcgg cgcccgtctc gccctttcca
    18841 ccggcacgct cagcacgctc ggctgggagc gcgagaccca ggtcatccgt tcgtggaata
    18901 gctgatggca accgggggct cacgcccctg gacccccgcc gtcacctggc cggtcggcta
    18961 ggcgactcgt cgcaatctca cgtgggcgtg gtgctcgagc gtctcggcga tcgcgcggtc
    19021 ggcctgtccg agcgcgaggt cggaggcgtg gggtgcgtcc gccagcggct ggccgagcgc
    19081 ctcgacgagg aagcggtgcg cctcggcacc gagcgggaac gagccggcct cgcagctggc
    19141 gcagaccacc ccgccggcag cgggcgagaa cccggccagg tgctctgcct cgccgcacga
    19201 ggcacatgac gccagctccg gcgcgaaccc cgacgccagc agcagcttga tccggaaggc
    19261 gagcgactgc gcccgcgtcg cagctgtggg atcgccgtcg agcagcgcga gctggttgca
    19321 gagcaggtgg taggccgcgg cgttcggctc acccgagtcg agcagcctca ggaccgactc
    19381 gcaagcctgc gaggcccgct cgagcgacga ccggtgctcg cgcaggccgg ggtgcgcgct
    19441 gaccgtgtcg accgccgaga ccgtggacag gtctcccctg ccctgacgca ggacaaggcg
    19501 cacgcgcgac agcggctcga gccggccgcc catccgcgag cgcgtccgcc gcacgccctt
    19561 tgccatcgcc ccgatgcggc cgcgatcgac gctgtagaga tgcagcacgc ggtccgcctc
    19621 gccgtagcgg atcgagcgca gtaccactgc ctccgtcttg aagctgccgg ccacctctcg
    19681 atgttgcagc cggccgcgga cagaagctct caggcccgcg gcggtctctg gcaccgcggg
    19741 cagatgtagg tgccgcggcc ggcggcgcgc agcttgacga tcgcggtccc gcaacgtggg
    19801 cagggctctc cctcgcgccg gtgaaccagg aagcggtcct ggaagctgcc gcgcgctccg
    19861 tcgacgtggc gaaagtcgtc gatcgacgct ccgcgggcgt cgatcccggc ctcgagcgac
    19921 tcgacgaccg cgccgtggag agcctcgacc tgcgggcggc gcagcgtccc gaccgggcgc
    19981 agcgggtgga tgcgcgcccg gaacagcgcc tcgtcggcgt agatgttgcc gacgccggcg
    20041 atccgctcct gggcgagcag gaacgccttg accggcgatc ggcggcccct ggcgagcgct
    20101 tgcagcgcct gcggcgtgaa gtcggcgctc agcggctcca cgcccagccg tgtcgagaag
    20161 tactcgtcgc gtgcgtcgtc gccgagcagc acgaggccgg tcccgaaccg ccgcggatcg
    20221 gcgaacagca cgctggagct gtcgtcgagc cgcagcctca cgcgcacgta tggctgctcg
    20281 ccggggtcgt ccgctcggac cagcagcagg ttgccggtca tccgcaggtg catcacgaga
    20341 tgcacctcgt cctcgaggac caggtcgaag tacttgccgc gccggccgac cgacaggatc
    20401 cgccggccct cgatcgcgtc ggcgacctcg agcggcgccg cgggctccga ccagcgcgcg
    20461 tcgaggacct cggcgcgggc gatcccccgc ccctcgagct gcggcgcgag ctgcctgcgg
    20521 atcgtctcga cctcgggtag ctccggcatc ggccagggct tctaccaggc gcggcgaatc
    20581 caggagcgtg gcaaccgtcg tcgcacccgc gcagagccgg ccgttcgagc acctagtgct
    20641 cgacttcgtc gcctacctcg agttcgagcg cggactgtcg cgcaacacgc ttgaggccta
    20701 ccgctccgac ctgctgcagt tcggccgctt cctggagcag cgcggcgtca cggcgctgac
    20761 cgccgagccg gtcgacatcg ccgacttcct ggcgagcctc ggcggcgtcg acggccgtac
    20821 ggcgtcgccg gcgacgataa accgcaagca ggcatgcctg cggtcgttct acaagcacct
    20881 gcgtcgcgag gagatcctct ccggcgaccc cacggccaag ctcgccgggc cgcgcaagag
    20941 ccgcaggctg ccgcaggtcc tgacccgcgg cgaggtcgcc aagctgctcg accagccgca
    21001 gggaaccgag ccgatcgcgc tgcgcgaccg cgccctgctc gagctgatgt acgcctgcgg
    21061 cctgcgcgcg tcggaggcga ccggcctcga ggtgaccgac ctcgacctcg acgagctgct
    21121 cctgcgcgcg cgcggcaagg gctcgaagga gcggctcgtc ccggtcggcc gcacggccgc
    21181 cgacgcggtc cgccgctacc tcgagcgcgg ccggccgcag ctcgtcggag cctcggtcga
    21241 ggctcgcctg ttcctcaact tccgcggcgg cgcgctgacc cgccagggcc tctacaagat
    21301 cgtccagcgc cacgcgcgct cggccggcct cggcgaccgc atgagcccgc acacgctgcg
    21361 ccacaccttc gccacccacc tgctcgccgg cggctgtgac ctgcgcgccg tccaggagat
    21421 gctggggcac gccgacgtcg cgacgacaca gctctacacc catctctcca gcgagcggct
    21481 gaaggacgtc tacttcagcg cccacccgcg cgcgacggcc ggctagcgga cctggaagcc
    21541 ctcgccggac aggcggccga tgtcgttggt caggccgatc gcgaacaggc cgatcacgag
    21601 catgaacccg accacccccg cgcgctccat cacgctgaac ggcaccggct tgccaccgcg
    21661 cagcttctcg acggcggccc agaagaggtg gcccccgtcg agcggcagga acgggaagag
    21721 gttcacgatc gccagcgaca gcgagatgat cgccaggatg ccgacggtgt tggcgaggtt
    21781 gttgaggatc gtctggcgcg tcacctcgta ggagccgacg atgccgctga tctccttgcg
    21841 ccgctcggtg tcgaatatgc gcgcgggcag gctgaccgtc tgctcggtta tgaaccagaa
    21901 gcggtcgagc gagacgctca gggcgtcgcc gatcggcagg gcctcgcgcg ggcccggcac
    21961 gtagctgaag ccgagacggg tccgctcggc ggccgggtcg gcgtcgtaga tcggcgtcag
    22021 ccgctcgacg atccgcctcc cgtcgcgctc gatcaccagc gtcgtcggcg cggcggcgcg
    22081 gcagccgtcg gtcggcggac tctgcgggca cttgtggctg gcgatctgct tcgacagcgc
    22141 ggtcggctcc ccgcgcacgc cgtcgaccga caggaggcgg tcgcccggct gcagggcagc
    22201 ggcggcggga tatcccttct cgacgatgtc gacgcgcgcg tcgaccggct cggtctccgc
    22261 tccgatcagc ccgaagtaga cgatcagcag cagcagcgcc acgatcaggt tgaccgccgg
    22321 tcctgcggcg atcaccgcca tccgcttcca gaccggctgg gcgtggtagg cgcgcgtgcg
    22381 aacctcgtcg gggaggtcct cggccgggct catgccggtg attcgcacgt agccgccggc
    22441 ggggatcagg ccgatcgcgt actcggtctc gccgaccttc ttgctggcca gagtcggcgg
    22501 gaagaagagc gagaacttct cgacgcgcat gccgacccac ttggcgaccg cgaagtgacc
    22561 cagctcgtgg aggatcacga gcagcgcgaa gccggcgaag gcaagcaacc agctcacgcg
    22621 agcgctcctt cgcaggcctc gcgggcccgt tcgcgcgcct cggcgtcggc cgcgtagagg
    22681 tcggagaagt ggcgcaccgg ccgctcgggc agagcggcga gcgtctgctc gatcacggct
    22741 ggaatgccga ggaacggcag cgcgccctcg aggaaggcgt gcaccgcgat ctcgttcgcg
    22801 gcgttgagga cgcacggagc ggtccccccc gcggccgccg cctcgcgcgc gaggcgcagg
    22861 cagggaaatg catcgccgtc gaccggctcg aagctcagcg caccgacctg cgcgaggtcg
    22921 agtggctcga ccggcaggtc gacgctctcg gcgccatgca gggcgtagga gatcgcgatc
    22981 cgcatgtccg gcggtccgac gtgggcgagc atcgcaccgt ctgcgagcgt caccagcccg
    23041 tggacgagcg actgcgggtg gacgaccacg tcgagccgct cgtagggcag cccgaagagg
    23101 tggtgagcct ccatcagctc gaggcccttg ttcatcaggg tcgccgagtc gatcgtgatc
    23161 ttgccgccca tctgccaggt cgggtgcgcg agcgcctggg cggccgtcac ctccgtgagc
    23221 tcgctgcgcg tgcggccgcg gaacggcccg ccgctggcgg tgatcgtcat ccggtcgacg
    23281 ccgccggtcg ggagcccggc gatcagctgg tgcagggcgg tgtgctcgga gtcgaccggc
    23341 acgatccggg cgccggtcgc ctccgcgagc gccatcacca gctcgccgcc cacgacgagg
    23401 ctctccttgt tggccagcgc caggtcgatc ccctcgccga gcgcagcgac ggtcggtccg
    23461 aggcccgcgg aaccaacgag cgagttgagc acgaggtcgc actccgcctc gacgatcagc
    23521 tcgacgaggc cctccgcacc gccgagcacg cggcccgacg tccagcgctc cgccgcgcgg
    23581 gccgccgcgt cgggatccgc gagcgcgatg cgatcgaccc catgcgcgga cgcctgttcg
    23641 agcagcgtgt cgacgctcga tcccgcggcc aggccgacga cctcgagcga gtcgctgcgc
    23701 gagatgacgt cgagtgcctg cgtgccgatc gagccggtcg agccggcgat gacgatcctg
    23761 cgcatccgcc tcagtctagg aggcgccccg cggtgcggca gcgagccgca gcagcagctg
    23821 caggcggatg cccgccggtg caagcgcagc cgtctcgccg tcggcgaata tctcgatcag
    23881 gtaggcgccc gagccgtacg cgacgacggc cacgagcgcg gcctcgaagc ccagatcggc
    23941 gagcagcttg cgctcggccg catcggtccg atcgtcctcg acgtgcgcga cgaagccgcc
    24001 gtggccgcgg cgcagcagct cggcggtgtt cgggaagtcc tcgacccggt agacctcgtt
    24061 cgcgagcccc atgcgcaggc cccgcaagcg ggtgtcgcgg tcatcggcgg tcgacaccga
    24121 gcggatcagc gagctcccgc gctcccagcg cgagatcgcc caggctgcgg cgtcgagcgt
    24181 cgccgaaaag gccacgacta ccgtctcgag ccggtcgagc actcggcggt gggcggcatc
    24241 acggtcgagg attcgcatcg tccgagccgc gatcgcctcg atctgctgat cgatccggtg
    24301 cgggctcagc tgacggcgct cgccgaggtc ggcggtcagc gggtcggccg ccggactcga
    24361 cgtgcagagg cgcccgccgc cgcgccgctt ggcggcgtac tgagcggtat cggcggcgtg
    24421 caacagctcg gccggcgtgc tggccggtcc cgcctcggcg gcgccgaagg agagcgacaa
    24481 cggcaagtcg gcatcggcgt cgtcgagcag ctcgagcgcc gtccgggcga ccgcgttggc
    24541 cgcgtcgatc cggcagccct cgagcagcac gcagaactcg tcgccggcga gtcgcccgac
    24601 gagcgctgcc gcctccggcg ccgcagcggc aaccagagcc tcgccgaccc ggcagagcgc
    24661 ccggtcgccg atgtgatggc cgtgtgcgtc gttgatgtcc ttgagaccgt cgacgtcggc
    24721 cagcagcagc gtcagtggag cgcgctggcg gccgtcggaa tcgagcagcg ccgcgagccg
    24781 ctcctcgatc gcgcgccggt tggcgaggcc ggtgagcgga tcctcgtagg cgagtcgcga
    24841 caccctcgcg aagcgctcgc cgcgcgcgag cacgagcgcc agccggtcgg cgatcgcctg
    24901 caggaagccg gcgtcatcgg cgtcgaagcg cggcgcgcct ggcgccgtag ccgcccagac
    24961 ctccccccaa acctctccgt cgagcaggat cggcacgccg acgtcggact ccttgccgag
    25021 cgccttcagg accgcgacgg cgggcggaga ggcacccggg tcgtcgactg cggtgcggta
    25081 aggctcgccg tcgcggagca gcttctcgac cagcggatgc tctgcgagcg gatagatctc
    25141 gtcggtcgga aagcgctgct cgtccggtcc cagctggccg acgttgatca gcgtccgcat
    25201 cacctcgcgg cccggctccc agcgactgat cgagagcgat gccgcgccgg tgacctcgag
    25261 cgccgcctcg gccgcctgct ccatcacatc ctcgagcctg taggcgtccg cggtggctgc
    25321 cgcgatccgc atcagcgcct cccgctcgcc agcctcgcgc cgcccgcggt cgcgaccgac
    25381 cgctcgcagc gggcgccgct gatgcgcggg gggctctacc tcccggagct cgtggggctg
    25441 gaccgacatc acgcactcca gctatcggcg cgcgcggcgc acaactgaag atctgccaac
    25501 tggcggtgcg ccagctgtct cttccgccga tactcagccg tagccgagca gcagcgccgc
    25561 gtagaacccg accacgatcg tcaggaagac ggcgtcgagg cggtcgaggg cgccgccgtg
    25621 ggccccgaag acgcgcccgc tgtccttgac cgagagatcg cgcttgacgg ccgactccag
    25681 caggtcaccg agcggcgccg cgagcgctac taggaagccg atcagcagcg cgtcgagacc
    25741 ggagagccag tcctggtaca aaccggcgca ccagaaggcc accgtagcgc cgacgatgcc
    25801 ggcgaccagc ccctcgaccg tcttgttcgg cgatatgtcc ggcgccagct tgcgccgccc
    25861 ccacatccgc cctccggtgt aggcggcggt gtcgccgacg aaggttgcga ccagcacgtc
    25921 gatcacgagc ccatcgccgt gttccagcga gcgcagaaag acggcgtggg ccagcggcag
    25981 cccgatccag accaccccga gcaccgtcgc cgccaacccc caggagacgt tcgccagcgt
    26041 cggccgcgcc atcgcaagca ggaaggcgag cggcagcgcc gcgaccagcg cgatcaggac
    26101 gtcggcgcgc tgctcgtagg tggccagcag caccatcccc gcgaccgcga ggtagccggc
    26161 caggtcgacc ggccgagcgc ggcgcatcag cccgtagagc tcgcggaggg ccacgacccc
    26221 gagcacgatc agcccgagcg cgaacaccag cccaccctgg gtcacgatga agatcgcaaa
    26281 cagcgccgcc ggaacggcga ccaacacccg gccggggaga tccgaccggg cctcctccga
    26341 ccggccaggc gccccggggg tccgggggcg cgtgcccccg gtcatgatcc cctccgggcg
    26401 ccgaagcggc gttggcggcg gccgtactcg gcgagggccc gctcgaaggc ggcgcggtcg
    26461 aagtcgggcc agagctcgtc gctgaacacg agctcggagt aggcgcactg ccagagcagg
    26521 tagttcgaga gccgctgctc gccgctggtg cggatcaaga ggtcggggtc gtgcatctct
    26581 ggcgcgtaga ggcgagcgcg gaactgctcc tcggtcgtgc cgtcgaagcc gcgtgcggca
    26641 tcgacgatct ccgcccgacc gccgtagttg aacgccacga acaaggtgat ccgggtgttc
    26701 tcgcgcgtcg ccgcctcggc ccactccatc ttctcgcgca gctcggcgga gattccgtcg
    26761 cgccggccga cgaagcgcat tcgaacgccc tccgcgtgca gctcgggggt ctcgccgtcg
    26821 atccgctcgc cgaacatccg catcagcccg gagacctcgt cgggcgaccg cttccagttc
    26881 tcggtcgaga acgagtagac ggtcagctcg cgcacaccga gctcggcggc atcgcgcagg
    26941 cgcgccttga ccacgtccgc cccggcgcgg tgcccctcga tcggcgacag cccgcgggcc
    27001 tgcgcccaac gtccgttgcc gtcggtgatg atcgcgacgt agcgcggcgt ccccgtgccg
    27061 ccgtcgccgt ccgtcggacc cgtcgccatc cgggtcagac ctggagtatc tcgtcctctt
    27121 tggcggcgag cgacgcgtcg atctcggcga ccttctcgtc ggtgagcttc tgcagctcgg
    27181 cctcggcacg gtgctcgtcg tcggcgccga tctcgccgtc cttcttcatg tcgcgcagct
    27241 cctgcattac ctcgcgacga acgttgcgga ccgcgacgcg gccctgctcg gcgagcccgt
    27301 gcacgacctt gacgagctcg cggcggcgct cctcggtcag ctccgggatc gacaggcgga
    27361 tcaggttgcc gtcgttgctc ggcgtcaggc cgacgtcgga ctcgaggatc gccttctcga
    27421 tcgccttgat cgcgcccttg tcgtacgggg tgatcgtcag cagccgcgcc tcggtcgccg
    27481 acacgttcgc cagctggttg agcggggtga ccgcgccgta gtagtcgacg tcgacgcggt
    27541 cgagcaggtg aggcgaggcg cggccggtgc ggacggtgcc gaactcgtgg cgagtcgagt
    27601 cgaccgtctt gcccatccgg tccgacgcgt cgtcgaggta ctccttgaca aggtcaccgt
    27661 ccatcgctgc tcaccaccgt ccccacacga tcgccgcaca ctatccggtc gatgttgcgc
    27721 tcgtcgtcca tgttgaagac gtggatcgga aggtcgttgt ccatgcagag cgacagcgcc
    27781 gtcgagtcca tcacgccgag ctggcgctcg atcgcctcgc ggtgactgat agccggcagg
    27841 aaggtcgcgt ccgccacctt gcgcgggtcc gcgtcgtaga cgccctcgac tccgttcttc
    27901 gccatcagga tcgcctcggc gcggatctcc agcgcacgca gcgcagcggc ggtgtcggtc
    27961 gtgaagaacg ggttgccggt gccggcggcg aagatcacta tccgcccacg ctcgaggtgg
    28021 cggatcgccc ggcggcggat gtagggctcg gcgacctcgg agatcgtgat cgccgactgg
    28081 acgcgcgtca ccgcctcctg cttctcgagc gcgtcctgca gcgcgagggc gttcaggacg
    28141 gtggccagca tgcccatgta gtcgccggtc gcccggtcca tccccttggc ggcgccggac
    28201 atgccgcgat agatgttgcc gccgccgacg acgatcgcga cctcgacgcc gcgtcgctgg
    28261 atgtgcgcga cctgggccgc gatcgcctgg atccgggcgg ggtcggcgcc gtagtccagc
    28321 tcgcccatca gggcctcgcc ggagagcttg agcaggacgc gcccgaacgc cgcctcgcgc
    28381 tcgggcgcgc tgccgtcgtc cccctcgccc ccggtcatag gcttactccc cgatcgcgaa
    28441 gcgggcgaag cggcggatga ccacgttctc gccggtcgtg gccgagatcg ccgcgcgcag
    28501 ctcctcgatc gtcttgccgt cgtgcttgtc ggcgttgacg tgagtctggt tgagcaggac
    28561 gacctcctcg agccacttct tcatccggcc ctcggcgatc ttctcctgga cctgcggcgg
    28621 cttgccctcc tcggcggcct gctcctggaa caccttcagc tcggcggcgc agtccgcctc
    28681 ggggacctcg tcctcgctga catagcgcgg cgcggccgcg gcgatgtgcg acgcgacgtc
    28741 gcgcgtgaac tcggcgaagt cctcgttgcg ggcgacgaag tcggtctcgc agtcgacctc
    28801 gacaagcacg ccgaccttgc cgttgtggtg gacgtagctg ctcaccaggc cctcggaggc
    28861 ggcgcgctcg ccgcgcttcg cggccttcgc ctggcccttg acgcgcagca gctcgatcgc
    28921 cttgtccatg tcgccgtcgg cctcgaccag ggcggccttg cagtccatca tccccgcgcc
    28981 ggtccgctcg cggagctcct tgacgctgcc tgcggtgatc gtgctcatga cttcgccccc
    29041 tcctcgggcg agaacgttcg ctgcccggcg ggcgccgggt cctcgtcggg cgagaagctg
    29101 cgctgctcgg cgggggccgg cgccggcggg ggctcggcgg gctgcccggc gggagcggcg
    29161 ggctgctcgc ccgacgccgc cgcggccgtc gcggcgtcct tacgcgcctg ctcctccgcc
    29221 tcgcgcttgg cggctgcctc ggcctcgatc cgggccttct cctcggcctc gcggcgggcc
    29281 tgctcctccg cctcgcgccg cgcggcctcc tcctgggcgc ggaacgccgc gtgctgctcg
    29341 ccgacggtgc cggcgatcgc ctcgatgatc agcttgcagg agcggatcgc gtcgtcgttg
    29401 ccgggaatga cgaagtcgac tggcgcgggg tcgcagttgg tgtcgaccag gccgatgatc
    29461 gggatcccga gccgctgggc ctccttgacc gcgatcgcct cggtcttgag gtcgacgacg
    29521 aacatcgcgt ctgggacgcg ctgcatgtcg cggacgccac cgaggttggt ggtcagcttg
    29581 tcgtgctcct tcttggcggc gatccgctca cgcgtcggca gcaggtcgag cgtgccggtc
    29641 tcggtccact cggtcagctc gtggagccgc ttgatccgct tgttgatcgt ctggaagttg
    29701 gtcagcaggc cgccgagcca gcgctggttg acgtacggca tgcccgacgc cagggcggcc
    29761 tcggcgatcg tgtcgcgagc ctgcttcttg gtgccgacga acagcaccgt cccgccgcgc
    29821 gcggcgatcg acgaggcgaa gtcctgcgcg ttggcgagca ggcgctcggt cttctgcagg
    29881 tcgatgatgt ggatcccgct gcgctcgccg aagatgaagc ggcgcatctt cgggttccag
    29941 cggcgcgtct ggtggccgaa gtggacgccg gcctccagca gctctctgag tgttacctga
    30001 gccaattcgg tgctcccttt ggttgtgcgt gcggtgatcc cgccggccgt cacgtggacc
    30061 cgagccatcc ggggctcggg cagggccacg ccgtactggc gggggtaggt tgcggtcggt
    30121 caagactacg gcaaccagcc aaggttcgcc ggagggggcc cgggggggg
//
LOCUS       DATABV010000122         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1966570, whole genome shotgun sequence.
ACCESSION   DATABV010000122 DATABV010000000
VERSION     DATABV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966570"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VHF88_05330"
     CDS             complement(<1..729)
                     /locus_tag="VHF88_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161227.1"
                     /translation="MFTGVSGSGKSSLVFATIAAESQRLINETYSAFVQGFMPTLARP
                     DVDVLDGLTTAIIVDQERMGANPRSTVGTVTDANAMLRILFSRLGQPHVGSPNAYSFN
                     VPSVRASGGITVERGGKTKTEKATFNRLGGMCPRCEGRGSVTDFDLSALYDDSKSLSE
                     GALTIPGYSMDGWYGRIFSGSGYFDMDKPIRKFNKRELHDLLHREPTKIKVEGINLTY
                     EGVIPKIQKSMLSKDPEAMQPHIRR"
     gene            complement(828..1232)
                     /locus_tag="VHF88_05335"
     CDS             complement(828..1232)
                     /locus_tag="VHF88_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2161228.1"
                     /translation="MIIHTTFLPHDDPDASLAFYRDVLGFEVRNQVEHGGMHWNTVGP
                     PDQPDVNIVLTPPGADPGITDEERKTIAEMMAKGTYASIMLATGDLDGTFGRIQAGDV
                     DIVQEPTDQPWGARDCALRDPAGNMIRIQERR"
     gene            complement(1249..1677)
                     /locus_tag="VHF88_05340"
     CDS             complement(1249..1677)
                     /locus_tag="VHF88_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2161229.1"
                     /translation="MLAVDLDELRYLRRARDRMDREYAEPLDVPALARTALMSTGHFS
                     RRFREAYGETPYSYLMTRRIERAAALLRRGDKSVTDACFAVGCSSLGTFSTRFAELVG
                     MPPSEYRRREAGAVAGMPPCLAKQVTRPIRNREARVAGPA"
     gene            1866..1997
                     /locus_tag="VHF88_05345"
     CDS             1866..1997
                     /locus_tag="VHF88_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161230.1"
                     /translation="MLLIGSTDGGRVLTLVIERTLDPTSWLMVTGWIATARERKIIG"
     gene            1999..2226
                     /locus_tag="VHF88_05350"
     CDS             1999..2226
                     /locus_tag="VHF88_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161231.1"
                     /translation="MGKDPWTDPDPQPGDFDAALDRVQPDQIEIHEGNPDARLTILVS
                     VEGDDAKRLERIAAERGQQPGEVVPQLLRSA"
     gene            complement(2276..2349)
                     /locus_tag="VHF88_05355"
     tRNA            complement(2276..2349)
                     /locus_tag="VHF88_05355"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2313..2315),aa:Asp,seq:gtc)
     gene            2420..3217
                     /locus_tag="VHF88_05360"
     CDS             2420..3217
                     /locus_tag="VHF88_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2161232.1"
                     /translation="MDLAGSRVLITGATGGIGMAIARRVHARGATVLLSGRQADVLER
                     LRAELAERAEVLVCDLADREQVAALPGRAGKVDVLVANAALPASGHLLDFEPGQIDRA
                     LDVNLRAPVMLARQLAPGMSDRGYGHIVLVSSLSGKAGSAGSSLYSATKFGLRGFGQG
                     LAEDLRGAGVGVTTVFPGFIRDAGMFHESGAKLPRYVGTSTPEKVAKAVVKGIESGKL
                     EIDVAPLGLRMGTTLAGLAPATVGAIQRRFGAHEIAAEIARGQVSKR"
     gene            3228..4004
                     /gene="budA"
                     /locus_tag="VHF88_05365"
     CDS             3228..4004
                     /gene="budA"
                     /locus_tag="VHF88_05365"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEX2161233.1"
                     /translation="MIDERLIRGLHVDATDLAGSRFASRVHEVFQTSTVAALVAGAFD
                     GDLSFAELARHGDLGLGTLNGLDGEMIALDGRFLRADVDGRINAINPSALTPFAVVTR
                     FSPTVSFDIDGPLDHRAFVKEVNRHAPPGSPSCAIRADGEFELVHARSVPRQQPPYRT
                     LAEAAADQHEFDFHDVAGTLVGFRFPDYAEGIELPGFHLHFVDAARERGGHVLACRPR
                     RLHVQIDHASDLHMELPPGVELPDPDAIADRAALRRIEGG"
     gene            complement(4098..>4314)
                     /locus_tag="VHF88_05370"
     CDS             complement(4098..>4314)
                     /locus_tag="VHF88_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161234.1"
                     /translation="NMGPRAYMRRRMAGILPEHLHYYYVGRQLRAAQSEGYTAAHRKE
                     QARIVRVALDLEDDVLKPDLSAERPKL"
BASE COUNT          602 a         1537 c         1521 g          654 t
ORIGIN      
        1 ccggcggatg tgcggctgca tcgcctcggg gtccttcgac agcatcgact tctggatctt
       61 cgggatcacg ccctcgtagg tcaggttgat gccctcgacc ttgatcttgg tcggctcgcg
      121 gtggagcagg tcgtgcagct cccgcttgtt gaacttgcgg atcggcttgt ccatgtcgaa
      181 gtagccggag ccgctgaaga tgcggccgta ccagccgtcc atgctgtagc cggggatcgt
      241 gagagcaccc tcgctgagcg acttgctgtc gtcgtacagc gcggacaggt cgaagtcggt
      301 gaccgagccg cggccctcgc agcgcgggca catcccgccg agccggttga aggtcgcctt
      361 ctcggtcttg gtcttgccgc cgcgctcgac ggtgatcccc ccactcgccc ggaccgacgg
      421 gacgttgaac gagtacgcgt tgggggagcc gacatgcggc tggccgagcc ggctgaagag
      481 gatccgcagc atcgcgttgg cgtcggtgac cgtgccgacc gtcgagcgcg ggttggcgcc
      541 catccgctcc tggtcgacga tgatcgcggt cgtcagcccg tcgaggacat cgacgtcggg
      601 ccgtgcgagc gtcggcatga agccctgcac gaaggcgctg taggtctcgt tgatcagccg
      661 ctgcgactcg gcggcgatcg tggcgaagac cagcgagctc ttgcccgagc cggagacgcc
      721 ggtgaacacc gtcagccggc gcttcgggag ctcgacgctg acgtccttga ggttgttctc
      781 gcgcgcgccg cggacgcgga tcaggtcgtg gctgtcggcg gcgtggctca gcgccgctcc
      841 tggatccgga tcatgttgcc ggcagggtcg cgcagggcgc agtcccgcgc tccccagggc
      901 tggtcggtgg gctcctggac gatgtcgacg tctccggcct ggatgcgccc gaaggtgccg
      961 tcgaggtcgc cggtggccaa catgatgctg gcgtaggtgc ccttggccat catctcggcg
     1021 atcgtcttgc gctcctcgtc ggtgatgccc gggtcggcgc ccggcggcgt caggacgatg
     1081 ttcacgtcgg gctggtcggg agggccgacg gtgttccagt gcatgccgcc gtgctcgacc
     1141 tggttgcgga cctcgaagcc gaggacgtcg cgatagaacg ccagcgacgc gtccggatcg
     1201 tcgtgtggga ggaaagtcgt gtgaatgatc aaatccccgg cggtcaggct aggcggggcc
     1261 ggcgacccgc gcttctcgat tcctgatcgg ccgggtcacc tgcttcgcca ggcacggcgg
     1321 catccccgcc acggcgcccg cctcccggcg ccggtactcg ctcggcggca ttccgaccag
     1381 ctcggcgaag cgagtgctga aggtgcccag cgacgagcag ccgaccgcga agcaggcgtc
     1441 cgtcacactc ttgtcgccgc gccgcagcag cgccgcggcc cgctcgatgc gccgcgtcat
     1501 caggtaggag taaggcgtct cgccatacgc ctcgcgaaag cggcggctga agtgcccggt
     1561 cgacatcagc gccgtgcggg ccagggcggg cacgtcgagc ggctcggcgt actcccgatc
     1621 catccggtcg cgggcgcgcc gcaggtaccg cagctcgtcg agatccacgg cgagcatctt
     1681 cccacggtgg actgcgcgag cgaccggact cgaaccggcg accgattgct tagctcctgg
     1741 cgaccgatgc ggcacgcgac aagctcggcc ggcgtggcat ctcgacggcc gaggccgagc
     1801 agctaccgcg gaaccgtcat gcgacggtgc gcaacccgca tggccggggt ggcgagggcg
     1861 agtgagtgtt gctcatcggt agtaccgatg gcggtcgcgt actgacgctg gtgatcgagc
     1921 gcaccctcga tccaacttcg tggctgatgg tcaccggctg gatcgccacc gcccgcgagc
     1981 gtaagatcat tggttgagat gggcaaagat ccttggacag atcccgatcc ccagcccggc
     2041 gacttcgacg ctgcgctcga ccgcgttcag cccgatcaga tcgaaatcca tgagggcaac
     2101 cccgacgcgc gtctgacgat cctcgtcagc gtcgagggcg acgacgccaa gcgcctcgag
     2161 cgaatcgccg ccgagcgcgg ccagcagcca ggcgaggtcg tcccgcagct cctgcgcagc
     2221 gcctgaccga cgcctagaac gacgaagccc gcctcagggg cgggcttcag cgagacgcga
     2281 gcgaccggac tcgaaccggc gacctccggc gtgacaggcc ggcgctctaa ccaactgagc
     2341 tacgcccgct gggggaccgc gaggatagcg gaggcggggc tgtgcccgga gcgccggagc
     2401 gctcggatag cctgcgcgcg tggatctcgc gggctcgagg gtgctgatca ccggtgcgac
     2461 cggtgggatc gggatggcga tcgcccgtcg cgtgcacgcg cggggagcga ccgtgctgct
     2521 gagcgggcgt caggccgacg tcctcgagcg gctgcgagcg gagctcgccg aacgggccga
     2581 ggtcctcgtc tgcgacctcg ccgaccgcga gcaggtcgcg gcgctgcccg ggcgcgccgg
     2641 caaggtggac gtcctcgtcg cgaacgcggc gttgccggcc agcggccacc tgctcgactt
     2701 cgagcccggc cagatcgacc gggcgctcga cgtaaatctg cgcgcgccgg tgatgcttgc
     2761 gcgccagctc gctccgggca tgtcggaccg cggctacggc catatcgtcc tcgtgtcctc
     2821 gctgtcgggc aaggcgggca gcgccggaag ctcgctctac tcggcgacga agttcgggct
     2881 gcgcgggttc ggccagggcc tcgccgagga cctccgtggc gcaggcgtcg gagtcacgac
     2941 ggtcttcccg ggcttcatcc gcgacgccgg gatgttccac gagagcggcg ccaagctgcc
     3001 ccgctacgtc ggcacgagca cgcccgagaa ggtcgccaag gcggtcgtga agggcatcga
     3061 gtcgggcaag ctcgagatcg acgtcgcgcc gctgggcctg cggatgggga cgacgctcgc
     3121 cggtctggcg cccgcgaccg tcggcgcgat ccagcgccgc ttcggcgccc acgagatcgc
     3181 cgccgagatc gcccgcggcc aggtctccaa gcgctgagtc cgcgcccgtg atcgacgagc
     3241 gcctgatccg cggcctgcac gtcgacgcca ccgacctcgc cggctcccgc ttcgcgagcc
     3301 gcgtccacga ggtcttccag acctcgacgg tcgcggccct ggtcgccggc gccttcgatg
     3361 gcgacctcag cttcgccgag ctggctcgcc acggtgacct cgggctcggc acgctgaacg
     3421 ggctcgacgg tgagatgatc gccctcgacg gccgcttcct gcgcgccgac gtcgacggcc
     3481 ggatcaacgc gatcaatccc tcggcgctga cgccgttcgc cgtcgtcacc cgcttctcgc
     3541 cgaccgtctc cttcgacatc gacggcccgc tcgaccaccg cgcgttcgtc aaggaggtca
     3601 accgccacgc cccgcccggc tcgccctcgt gcgcgatccg cgccgacggc gagttcgagc
     3661 tcgtccacgc ccgctcggtc ccgcgccagc agccgccgta ccgcaccctc gccgaggccg
     3721 ccgccgacca gcacgagttc gacttccacg acgtcgcggg cacgctcgtc ggcttccgct
     3781 tccccgacta cgccgagggc atcgagctgc ccggcttcca cctccacttc gtcgacgccg
     3841 cccgcgagcg cggcggccac gtcctcgcct gccgtccgcg ccgcctccac gtccagatcg
     3901 accacgccag cgacctgcac atggagctcc cgcccggcgt cgagctgccc gaccccgacg
     3961 cgatcgccga tcgcgcggcg ctccgccgca ttgagggcgg ctagcgccac ccctggagcc
     4021 gagatgtagg aaccctcgcc ggagccgtcg gcagaccgac accgcgcggg ggctccgggg
     4081 gccgcgcgtc ccccggtcta caacttcggt cgctcggcgg acaggtccgg cttgagcacg
     4141 tcgtcctcga ggtcgagcgc gacgcgcacg atgcgcgcct gctccttgcg gtgcgcggcg
     4201 gtgtagccct cgctctgggc ggcgcggagc tggcggccga cgtagtagta gtggaggtgc
     4261 tcgggaagga tgccggccat gcggcggcgc atgtaggcgc gcgggcccat gttg
//
LOCUS       DATABV010000123         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1565810, whole genome shotgun sequence.
ACCESSION   DATABV010000123 DATABV010000000
VERSION     DATABV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565810"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..831)
                     /locus_tag="VHF88_05375"
     CDS             complement(46..831)
                     /locus_tag="VHF88_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161235.1"
                     /translation="MFRQLNRFRVGQGSKVKAALGASIVLAVVISPFAVAQTSGLIGG
                     KRNPRAGAYDTETKVIATNNTWGMRYSNRTVGGGGGVLFGCRSEPGGTPQRNYPCMRS
                     RNVANGLAFEMVTAGLLGGTISVGSGGDNAKPFTTNATGVATGLNAERVGSKTPAQLT
                     SDALSAVQGTLSFAQVSAAGASPAHRGVSSVGHPSTGTYTVTFANPVNACALTATQSA
                     SGTDVGTIGTELGADNRTVTVTTLGLIPEPALADRAFSISAVC"
     gene            968..1909
                     /locus_tag="VHF88_05380"
     CDS             968..1909
                     /locus_tag="VHF88_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161236.1"
                     /translation="MRVLLWISFPLALLLAPTTAAAQGQPPGFKATSLAPVPGGGDVA
                     ECHMDGQVLRCLLYGAEAEGASCDAGGPVLRMTLPPKGPARRDHACVDEAFHGWRELG
                     RNRSWDHESFHCGRYYAAAPGVVGASDVVRCVNASDRGFTLDGHGTTRLVSRAFRKRV
                     AATPRRGVVSVAGLPIAGVPRSMRRSVTSNEVDARIGPADSYDAVPGRCHSRWSDGLE
                     MVFASFAGDTRCRRLKLQEATLSGEGWRVRVGRRVYRTGDSAARISSNARRYRRWDGA
                     FALASMPFAGGRTPAVLAHVSKGRIDRFFLFIGGAGD"
     gene            2129..>2487
                     /locus_tag="VHF88_05385"
     CDS             2129..>2487
                     /locus_tag="VHF88_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161237.1"
                     /translation="MAPAGGALEIAGTDASETLVVTQASGTYTVAGDAPVAPFGALGA
                     CVQGSGPNEVVCTPTGPVEVLSVNLADGDDSLTVARVGQTGALPTSVEQLIAGGAGDD
                     TLRAGDGAVTFLESGAPP"
BASE COUNT          344 a          859 c          910 g          374 t
ORIGIN      
        1 cgcgacggcc atggcggccg tcgcggggcc cgaacggtcg gaggatcagc agacggccga
       61 gatgctgaag gcgcggtcgg ccagcgccgg ctcggggatc aagccgagcg tcgtgaccgt
      121 cacggtccgg ttgtcagcgc cgagctccgt gccgatggtg cccacgtcgg taccggatgc
      181 cgactgggtc gccgtgagcg cacaggcgtt gaccgggttc gcgaacgtga ccgtgtaggt
      241 cccggtcgac ggatggccga cgctgctcac cccccggtgg gcggggctcg cgccggcggc
      301 cgacacctgg gcgaagctca gcgtcccctg caccgcggac agggcgtcgc tggtgagctg
      361 ggcgggggtc ttcgagccga cccgctcggc gttgaggccc gtcgccacgc cggtcgcgtt
      421 cgtcgtgaac ggcttggcgt tgtcgccgcc gctgccgacg ctgatcgtcc cgccgagcag
      481 tcccgcggtg accatctcga aggcgagacc gttggcgacg ttgcgcgagc gcatgcacgg
      541 atagttgcgc tgcggggtgc cgccgggctc ggagcgacag ccgaagagca cgccgccgcc
      601 accgccgacc gtgcggttgg aatagcgcat gccccacgtg ttgttcgtgg cgatcacctt
      661 cgtctcggtg tcgtaggcac cggcccgcgg gttgcgcttg ccgccgatca ggccgctcgt
      721 ctgcgcgacg gcgaagggcg agatcaccac cgcgagcacg atcgacgccc ccagcgccgc
      781 cttgaccttg ctgccctgcc caaccctgaa ccgattcagc tgtctgaaca ttcggacgtt
      841 tcctcctgat agtcgacgtc gagtcgttca tgggccccgg ccttgccgac ggccccggag
      901 gaggtaaacc ggcagttgcg cccgtgttgc gctttgtgcc cgcggctgcc gacgatatcg
      961 tcgcgatatg cgcgtactgc tctggatctc cttccccctc gcactgctcc tcgcaccgac
     1021 gacggccgcc gcccagggcc aaccgcccgg attcaaggcg acctcgctgg cgccggttcc
     1081 cggcgggggc gacgtcgcgg agtgccacat ggatgggcag gtcctcagat gtctcctata
     1141 cggcgccgag gccgagggag cgagctgcga cgcgggcggg ccggtcctcc gcatgacgct
     1201 gccgccaaag gggcctgcgc gccgggatca cgcctgcgtg gacgaggcct tccacggatg
     1261 gcgcgagctg ggacggaacc gcagctggga ccacgagagc tttcactgcg gccgctacta
     1321 cgccgccgcc cccggtgtgg ttggcgcgtc ggacgtggtg cgctgcgtga acgcaagcga
     1381 caggggattc actctcgacg gtcacggcac cacgcgcctg gtgtcgcgcg ccttccggaa
     1441 gcgcgtggcg gccaccccgc gcaggggcgt ggtctcggta gccggcctgc cgatcgcggg
     1501 cgttccgcgc tccatgcgcc gcagcgtgac ctcgaacgag gtcgacgcga ggatcggtcc
     1561 ggcggactcc tacgacgccg tgcccggccg gtgccattcg aggtggagcg acgggctcga
     1621 gatggtcttc gcgagcttcg cgggcgacac ccgctgccgg cgcctgaagc ttcaggaggc
     1681 gacgctcagt ggcgagggat ggcgggtcag ggtcggccgg cgcgtgtaca ggacgggaga
     1741 cagcgcggca cggatctcga gcaacgcgag gcgctaccgg cgctgggacg gcgccttcgc
     1801 cctcgcctcg atgccctttg ctggaggacg cacgcccgca gttctcgcac acgtgtcgaa
     1861 ggggcgaatc gaccgcttct tcctcttcat cggcggcgcc ggagactgaa gagcaacgag
     1921 tcacgcccaa tggaaacggt agtgtgtccg ccgcgatggg accagaatgg cgcgcggcgc
     1981 tacccggcca ggccggaaac gggagcgctt gaaagccgcc agggaaagcg gaaccatctg
     2041 ccgatccgct cgcaaggcgg cggtcggttt cgtgcttgtt gcgatcacgc tgcttggggc
     2101 tgagtcggcg ggtgccgcga cggtcgcctt ggccccggct gggggcgcgc tcgagatcgc
     2161 cggcaccgac gcgagcgaga ctctcgtcgt cactcaggcg agcggcacct acaccgtggc
     2221 gggcgacgca cccgtcgctc cgttcggcgc tttgggcgcc tgcgtgcagg gctcgggccc
     2281 gaacgaggtc gtgtgcacgc ccactggccc tgtggaggtc ctcagcgtca acctcgccga
     2341 cggcgacgac tcgctgacgg tcgcgcgcgt gggtcagacg ggagccctgc cgacatcggt
     2401 cgagcagctg atcgcgggcg gcgccggcga cgacaccctg cgcgccggcg acggcgcagt
     2461 gacgttcctc gagtcgggcg cgccccc
//
LOCUS       DATABV010000124         5501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1083847, whole genome shotgun sequence.
ACCESSION   DATABV010000124 DATABV010000000
VERSION     DATABV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083847"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VHF88_05390"
     CDS             complement(<1..525)
                     /locus_tag="VHF88_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2161238.1"
                     /translation="MSAGVAVAVDDLAASYGGPSPVLEGVDFSLPAGSSLCVLGPNGG
                     GKTTLFRALTGELTPLRGSLRVDGRPAYLAQTERTRLDFPVSALDVALMGSLAGGRWW
                     LPARRSERAAARGALARVGLEERADVRFGELSGGQRQRVLIARSLVQEARVLLLDEPL
                     SGVDPASAEGLARLF"
     gene            complement(522..1388)
                     /locus_tag="VHF88_05395"
     CDS             complement(522..1388)
                     /locus_tag="VHF88_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX2161239.1"
                     /translation="MSLLASLPFPGDLVELPFMRTALIELTLLGVAGGLLGAWIVLRR
                     LAFFAHAVGTATFPGLVVADATGFSATLAGLAVALGYAGGVERTGRAGRDPGDVATAL
                     ALVVALALGVVLASDVFESGAAVDRLLFGSALGLDAVDLWMAGGAAALALAATLLLGR
                     AWTAAGFDASGAASSGVPVHRADLLLLALIAVAAVVALPTVGALLVASLFVVPAATAR
                     QVTGGIGSLLAASVAVAVAQGALGLYVSLWLDVPPGPAVAVVGAGAYTLVATTCALGR
                     RSRVAPLAAETA"
     gene            1467..2765
                     /gene="lysA"
                     /locus_tag="VHF88_05400"
     CDS             1467..2765
                     /gene="lysA"
                     /locus_tag="VHF88_05400"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX2161240.1"
                     /translation="MGRDDAEQALADPGRADALPGGVGHVYPLGSRINAAGRLEIGGC
                     DVLELAREFGTPAYVYAEDDLRQRARAIQDAFAARTDDFEVLYASKALPCTPALRIFA
                     AAGLSCDVASGGELHLALRAGFDPERIYMHGNNKSPGELEYALESGVGHVIVDSLDEI
                     DRLERVAAGRRQRVLLRVTPGIRPETHYRIATGQEDSKFGLPLATLPRALERCAAAGL
                     EVRGLHAHIGSQVFDLDVYDTLAEVLAAAGDFPVINMGGGFAVAYTRDQRPPSADEFA
                     EKMLERLPDGIKVLCEPGRSLVANGGVTLYTVGTVKRIEGIRTYVAVDGGMADNIRPM
                     LYDAVYEAEIADRFGDSTPCRLVGMHCESGDVLIEEAALEDPRPGDLLVTPATGAYGH
                     AMASNYNAVRRPPVIFCRDGDARAVVRRETYDDLTSRELE"
     gene            2752..3981
                     /locus_tag="VHF88_05405"
     CDS             2752..3981
                     /locus_tag="VHF88_05405"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX2161241.1"
                     /translation="MSSNRPFRIGLLGRGTVGSAFEALLEQRADAIAAGVGRRPEIAG
                     VLTRSQGDYADILERSDLIVEVMGGIDPALDHVTRALEARKHVVTANKQLLSQHGEQL
                     YAAAAASGTQLGFEAAVAGVVPAIRVMRESLAAAHIERVHGIVNGTTNFILTAMARTG
                     ASYDDALREAQELGFAEADPTEDVTGKDAAAKMAILARLAFGARVSLADVPYEGIEHV
                     TADDIAYARELGLSLKLIGSAERIDGGIAVAVYPAFLYDGHPLATVSGSYNAVTIESP
                     AITEITLSGPGAGGSQTASAVLGDVVAAMTSPAIHAPPVAELPIVTDVESAFYLYVEV
                     ADRPGVLAQVAEILGLQGISVKSVVQRGIGDDARLAMVMHPVLESKFRAAVDMIERLD
                     FVRSRPRSIHVIEEVFE"
     gene            4073..5071
                     /gene="thrC"
                     /locus_tag="VHF88_05410"
     CDS             4073..5071
                     /gene="thrC"
                     /locus_tag="VHF88_05410"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2161242.1"
                     /translation="MSLWRYRDRLPVAPLVSLEEGDTPLVPAPWLSERTGCEVHLKLE
                     GANPTGSFKDRGMTVAVSAAIEEGAKAVICASTGNTAASAAAYAARAGLTGAVIVPEG
                     KIATGKLAQALMHGARVIALRGNFDQALALVRELTDRHPIALVNSVNDYRIEGQKTGA
                     FEVCDQLGEAPDVLCIPVGNAGNVTAWWKGFGEYEAAPELHGYQAAGAAPLVEGAPVQ
                     APETVASAIRIGNPARWEDAMSAFTSSRGQVRAVTDEQILDAYRLLASREGVFCEPAS
                     AASVAGLLAHGAHGRVVCVLTGHGLKDPQTAMDRATSVVPCEPDIASVEAAVLGDG"
     gene            5092..>5501
                     /locus_tag="VHF88_05415"
     CDS             5092..>5501
                     /locus_tag="VHF88_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161243.1"
                     /translation="MPASSANLGPGYDVFAAALSLSLELEVEETGTFSVESDTPGVPL
                     DRDNLCVRAFERLHSADGIAFRIRSEIPASAGLGSSAAAIVAGLAAADHLYELDAPLL
                     EHATALEGHPDNVAAALLGGFVVCADGDPLRLEPP"
BASE COUNT          781 a         2009 c         2013 g          698 t
ORIGIN      
        1 gaacaggcgg gccaggccct ccgcgctggc cgggtcgacg cccgagagcg gctcgtcgag
       61 cagcaggaca cgtgcctcct ggaccagcga gcgcgcgatc agcacgcgct ggcgctggcc
      121 gcccgagagc tcgccgaagc ggacgtcggc gcgctcctcg aggccgaccc gcgcgagcgc
      181 gccgcgcgcc gccgcccgct cggagcgccg cgccggcagc caccagcggc cgccggcgag
      241 cgagcccatc agcgcgacgt cgagcgcact gaccgggaag tcgagccgcg tccgctcggt
      301 ctgggcgagg taggcggggc ggccgtcgac gcgcaggctg ccgcgcagcg gggtgagctc
      361 gcccgtcagg gcgcggaaca gcgtcgtctt gccgccgccg ttcgggccga ggacgcagag
      421 cgagctgccg gccgggagcg agaagtccac cccttctagc acgggggagg ggccgccata
      481 gctcgccgcc aagtcgtcga cggctaccgc gacgccggcg ctcatgcggt ctccgcggcg
      541 agcggggcca cccgggaccg gcggccgagc gcgcatgtgg tcgccaccag cgtgtaggcg
      601 cccgctccga ccacggcgac cgccgggccg ggcggcacgt cgagccagag cgagacgtag
      661 aggccgagcg caccttgcgc gacggcaact gctaccgacg cggcaagcag gctgccgatg
      721 ccgccggtca cctggcgcgc cgtcgcggcc gggaccacga acagcgaggc gaccagcagg
      781 gccccgaccg tgggcagcgc gacgaccgcg gcgacggcga tcagcgcgag cagcagcaga
      841 tcggcacggt gcaccggcac gcccgacgag gccgcgccgg acgcgtcgaa gccggctgcc
      901 gtccaggccc ggcccagcag cagcgtggcg gcgagggcga gcgcggctgc gcccccggcc
      961 atccagaggt cgacggcgtc gagtccgagc gcgctgccga acagcagccg gtcgaccgcg
     1021 gcgccggact cgaagacgtc gctggcgagc accaccccga gcgcgagcgc gaccacgagc
     1081 gccagcgcgg tcgcgacgtc gccggggtcg cggccggcgc gcccggtccg ctcgaccccg
     1141 ccggcgtagc cgagcgcgac cgctaggccg gcgagcgtgg cgctgaagcc ggtcgcgtcg
     1201 gcgacgacca gtccgggaaa ggtggcggtc ccgacggcgt gggcgaagaa ggcgagccgg
     1261 cgcagcacga tccacgcccc tagtaggccg ccggccacgc cgagcagtgt gagctcgatc
     1321 agcgcggtgc gcatgaaagg cagctcgacg aggtccccgg ggaagggcag cgaggcgagc
     1381 aggctcacac gtgaagcata acgagaatca ttctcgttat cggcctcacc acgcagattt
     1441 gctcgtgctt cataatccgc acgttcatgg gccgagacga cgcggagcag gcgttggcgg
     1501 atccggggcg ggccgacgcg ctccccggag gggtcggtca cgtctatccg ctcgggtcgc
     1561 ggatcaacgc ggccggacgg ctcgagatcg gcggctgcga cgtcctcgag ctggcgcgcg
     1621 agttcggcac cccggcgtac gtatacgcgg aggacgacct gcgccagcgg gcgcgggcga
     1681 tccaagacgc gttcgccgcg cgcaccgacg acttcgaggt cctctacgcg agcaaggcgc
     1741 tcccgtgcac gccggcgctg cggatcttcg ccgcggcggg cctgtcctgc gacgtcgcct
     1801 ccggcggaga gctgcacctc gcgctgcgcg ccggattcga tcccgagcgg atctacatgc
     1861 acggcaacaa caagtcgccc ggcgagctcg agtacgccct cgagagcggc gtcggccacg
     1921 tcatcgtcga ctcgctcgac gagatcgacc ggctcgagcg cgttgccgcc ggtcgtcgcc
     1981 agcgtgtgct gctgcgggtc accccgggga tcaggcccga gacgcactac cgcatcgcga
     2041 ccggccagga ggactcgaag ttcggcctgc cgctggcgac gctgccgcgc gcgctcgagc
     2101 gctgcgcggc cgccgggctc gaggtgcgcg gcctgcacgc gcacatcggc tcgcaggtct
     2161 tcgacctcga cgtctacgac acgctcgccg aggtgctggc cgccgctggc gacttcccgg
     2221 tgatcaacat gggcggcggc ttcgcggtcg cctacacgcg cgaccagcgc ccgccgtccg
     2281 ccgacgagtt cgccgagaag atgctcgagc ggctgccgga cgggatcaag gtgctctgcg
     2341 agccgggccg ctcgctggtc gccaacggcg gcgtgacgct ctacacggtc gggaccgtca
     2401 agcggatcga gggcatccgg acctacgtag ccgtcgacgg cgggatggcc gacaacattc
     2461 gcccgatgct ctacgacgcc gtctacgagg cggagatcgc cgaccgcttc ggcgactcga
     2521 ccccttgccg gctggtcggg atgcactgcg agtccggcga cgtgctgatc gaggaggcgg
     2581 cgctcgagga tccgcgcccc ggcgacctgc tcgtgacgcc ggcgacgggc gcctacggac
     2641 acgcgatggc gagcaactac aacgctgtgc gccggccgcc ggtgatcttc tgccgcgacg
     2701 gcgacgcacg cgccgtcgtc cgccgtgaga cctatgacga cctgacatcg cgtgagctcg
     2761 aataggccgt tcaggatcgg gctgctcggg cgcgggaccg tcggctccgc gttcgaggcg
     2821 ctgctcgagc agcgcgccga cgcgatcgcg gcgggggtcg gccggcggcc cgagatcgcc
     2881 ggcgtgctta cgcgcagcca gggcgactac gccgacatcc tcgagcgcag cgacctgatc
     2941 gtcgaggtga tgggcgggat cgacccggcg ctcgaccacg tcacccgcgc gctcgaggcg
     3001 cgcaagcacg tcgtgaccgc caacaagcag ctgctgtccc agcacggcga gcagctctac
     3061 gccgcggccg ccgcgagcgg gacccagctc ggcttcgagg ccgcagtcgc gggcgtcgtc
     3121 cccgcgatcc gggtgatgcg cgagtcgctc gccgccgccc acatcgagcg cgtccacggg
     3181 atcgtcaacg gcaccaccaa cttcatcctg accgcgatgg cgcgcaccgg cgcgtcctac
     3241 gacgacgcgc tgcgcgaggc ccaggagctc ggcttcgccg aggcggaccc gaccgaggac
     3301 gtgaccggca aggacgccgc cgcgaagatg gcgatcctcg cgcggctcgc cttcggcgcc
     3361 cgcgtcagcc tcgccgacgt cccctacgag gggatcgagc acgtcaccgc cgacgacatc
     3421 gcctacgcgc gcgagctcgg cctgtcgctg aagctgatcg gcagcgccga gcggatcgac
     3481 ggcggcatcg cggtcgcggt ctacccggcc ttcctctacg acggccaccc gctggcgacg
     3541 gtctcgggct cctacaacgc ggtgacgatc gagtcgccgg cgatcaccga gatcacgctc
     3601 tccgggccag gcgccggtgg cagccaaacg gcctctgccg tcctcggcga cgtcgtcgcc
     3661 gcgatgacgt cgcccgctat ccatgcgcct cccgtcgcgg agctcccgat cgtcaccgac
     3721 gtcgagtcgg ccttctacct ctacgtcgag gtcgccgacc gtccgggtgt gctcgcccag
     3781 gtcgcggaga tcctcggcct ccaggggatc tcggtcaagt ccgtcgtcca gcgagggatc
     3841 ggcgacgacg cccggctggc gatggtcatg catcccgtgc ttgagtcgaa gttccgcgcc
     3901 gctgtcgaca tgatcgagcg gctcgacttc gtccgctcac gcccgcgctc gatccacgtg
     3961 atcgaggagg tatttgagtg agctgcggtg ctcacgggac ctcgctcccg ctagctgcgc
     4021 ggcgtcgccg taggaccggt cccgctgcgc tcctccgctt gagggtcgcc ggatgagcct
     4081 ctggcggtac cgggatcggc tcccggtggc gccgctggtc tcgctcgagg agggggacac
     4141 gccgctggtg ccggcgccgt ggctgtcgga gcggaccgga tgcgaggtgc atctcaagct
     4201 cgagggggcg aacccgaccg ggtcgttcaa ggatcgcggc atgacggtcg cggtctcggc
     4261 tgcgatcgag gagggcgcga aggcggtcat ctgcgcgtcg accggcaaca cagcggcgag
     4321 tgcggcggcc tacgccgccc gcgccggcct gacgggcgcg gtgatcgtgc cggagggcaa
     4381 gatcgcgacc ggcaagctgg cccaggcgct gatgcacggc gcgcgcgtga tcgcgctgcg
     4441 cggcaacttc gaccaggcgc tggcgctggt ccgcgagctg accgaccgcc acccgatcgc
     4501 actcgtgaac tcggtcaacg actaccggat cgagggccag aagaccggcg ccttcgaggt
     4561 ctgtgaccag ctcggtgagg cgccggacgt cctctgcatc ccggtcggca acgccggcaa
     4621 cgtcaccgcc tggtggaagg ggttcggcga gtacgaagcg gcgccggagc tgcacggcta
     4681 ccaagccgcg ggcgcggcgc cgctggtcga gggcgcgccg gtgcaggcac ccgagaccgt
     4741 cgcgtcggcg atccggatcg gcaacccggc gcgctgggag gacgcgatga gcgccttcac
     4801 ctcctcgcgc ggccaggtgc gcgcggtgac cgacgagcag atcctcgacg cctaccggct
     4861 gctcgcatcg cgcgagggcg tgttctgcga gccggcgtcc gccgcgtcgg tcgccgggct
     4921 gctcgcccac ggagcgcacg gacgcgtcgt ctgcgtgctg accggccacg gcctcaagga
     4981 tccgcagact gcgatggacc gcgcgacgtc ggtcgtgccg tgcgagccgg acatcgcgag
     5041 cgtcgaggcg gcggtcctgg gggacgggtg aaccgccgcc gtaccgttcg cgtgcccgcg
     5101 tcgtcggcca acctcggccc gggctacgac gtcttcgcgg ccgcgctatc gctgtcgctc
     5161 gagctggagg tcgaggagac cggcaccttc tcggttgagt ccgacacgcc gggcgtcccg
     5221 ctcgaccgcg acaacctctg cgtgcgcgcg ttcgagcgcc tgcattccgc cgacggcatc
     5281 gcgtttcgga tccgctccga gatcccggcg tcggccgggt tgggctcgag cgccgcggcg
     5341 atcgtcgcgg gcctcgccgc cgccgaccac ctctacgagc tcgacgcgcc cctgctcgag
     5401 catgcgacgg cgctcgaggg ccatcccgac aacgttgccg cggcgctgct gggcggcttc
     5461 gtcgtctgcg ccgacggcga cccactgcgg ctcgagccgc c
//
LOCUS       DATABV010000125         5486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_201385, whole genome shotgun sequence.
ACCESSION   DATABV010000125 DATABV010000000
VERSION     DATABV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201385"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VHF88_05420"
     CDS             complement(<1..624)
                     /locus_tag="VHF88_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161244.1"
                     /translation="MSPPPLSLAPDPPPEAGGEPLDLAVIADRIGWEEKAILAAARER
                     GARAEWLDDGRLCCGSREDLPAAGAYLIRSRSFVRGPLIAESIASGPGDPSVINSAEV
                     IGICSNKVTALAKLGQVGLPTVPYRLVLTRADLTAALGVLGCPLVLKPVYGGLGRRVM
                     LVRERAVADSMYDYVEHYGQNFDRALIAQPYLEGASDVRVLAIDGEAA"
     gene            complement(621..1931)
                     /gene="lysA"
                     /locus_tag="VHF88_05425"
     CDS             complement(621..1931)
                     /gene="lysA"
                     /locus_tag="VHF88_05425"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX2161245.1"
                     /translation="MLLGTQEVGGDGVLRVGGCSTLDLAEEHGTPVYLYDEAEIRRMC
                     RAYVDGFTARWPHVDVSYACKAFTAPAMMELALSEGLGLEVVSVGEMHMALHCGCPPE
                     LITYHGTFKKEPDLEFAIDADCGVIVIDNLSEIAPIARVAERLGKVQPVLVRINPTVT
                     GLENPEFLTVGDDTKFGLSIEHGFAGMAIDRALSEPSLRVDGIHFHLSSQQQRFGPYR
                     QAMERVAEVLARAQERHPDFRPRRIVAGGGRGVRYRPSDDPAPVEDWVEAIAGTFADK
                     VAPMCAEDVRLGIEPGRSVVAEGGVMLYTVGPIKPAGPPGDERAYYVIVDGGLSDNPR
                     HLMYDAHHEVKLARDPFGEPAMRVTICGRHSEPDILFPDRMLPELRSGDVLAVQSTGA
                     YTVNMSSNYMGFLRPPVVFVKDGESRVVVRREQVDDLLATSLAL"
     gene            2072..3223
                     /locus_tag="VHF88_05430"
     CDS             2072..3223
                     /locus_tag="VHF88_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161246.1"
                     /translation="MKISDATLRDTAHMAGVAFDADDARVLAGLLCDVGVDIVEAGIV
                     SDADRSEVPLVQAVVEVAGRERTKTVILARSRRQVEEDLDAALETGAGNVMLSIPTSP
                     THAMLKLQTDSERRIMTLAARTIEAAKSVGLSVTFSAEDGARTDPGFLERYVAAGAEA
                     GADRFRLAETVSSLAPNDVGPLIERLVAAAGPTEVEMHSHNMLGLSVANALAAADAGA
                     AWISCTIDGLGERGGNTPLAEILCVLRVLRGVDRFDLRPLYDLSREAAARAGLRCSAT
                     TGPTAELSYQYEIPGQVRNPEAFELLPPEAVGNRRGVRIGSRLKPSLLEAFLPEETLD
                     GADAVEVTERIRASAPHDKHVWDLATLDELARIHRGELRADAADVPVTN"
     gene            3293..3415
                     /locus_tag="VHF88_05435"
     CDS             3293..3415
                     /locus_tag="VHF88_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161247.1"
                     /translation="METGETLVCRECQLTLQVRGQEGETLVLEPVEASLSDWGE"
     gene            3412..>5486
                     /locus_tag="VHF88_05440"
     CDS             3412..>5486
                     /locus_tag="VHF88_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2161248.1"
                     /translation="MSAAAENGAPGEVAAPEPSYDARAVEARRQRDWRERDAFATPPP
                     DPDRPDAYVKAVCPFTSGHIHVGHVRSYTIADAYARWRRMSGDAVLFSIGFDAFGLPA
                     ELAAIANKESPADWVQRSYEHMLRQFERMGFSFDWERVFVSSDEEQYRWSQWLFLAFL
                     DAGLLYQEEATVDWCDSCQTTLASLQVEDGRCWRCNGPARLIKRKQWFLRISAYLEQN
                     ERGIEDHAGKWDRPALTAQRAILGRVDGVEIDLHGADGALTCFTEHPDAIGQARFVLV
                     SPRHPELERLLGDRPGVLAELEEVRQGGWQRGDRSAESVPAIDSGVRLTAPNGAELPL
                     VISPAVDGRYGPTAVLGIPAADESDGVIARRLDLPRPEPTAPTSNGKAPRPAVRYRAG
                     DFAISRQRSWGAPIPIVHCDDCGEVPVRKEDLPVRLPRDLKPTGEGNPLAAREDFMAT
                     TCPRCGADARRETDTLDCHFDDLWMWLPTCVPKADRDRQMFDHPECERWLPGAMLIHG
                     ADGGGYFFDQRITAKALRDIGPLAHLTDGEQFTGAVMHEMVTQDGRKMSKHLGNVVDP
                     DDLMERVGADTTRLAVLHGARPSRALNWTENAVQYCAKFLSDLWAYAAERLPGPDPHE
                     AAAAPDPDGAPQRRERLEGWVATAAAKVTEDLERVEMQRAVKNAMRLFDRIEEFETKS
                     RRANRGDLAA"
BASE COUNT          792 a         2020 c         1958 g          716 t
ORIGIN      
        1 ggccgcctcg ccgtcgatcg ccagcacgcg cacgtcggac gcgccctcga ggtacggctg
       61 ggcgatcagc gcgcggtcga agttctggcc gtagtgctcg acgtagtcgt acatgctgtc
      121 ggcgaccgcg cgctcgcgca cgagcatcac gcgccggccg aggccgccgt agacgggctt
      181 gagcaccagc gggcagccga gcaccccgag cgccgccgtc aggtcggcgc gcgtcagcac
      241 cagccggtac ggcaccgtcg gcagcccgac ctggcccagc ttcgccagcg ccgtcacctt
      301 gttcgagcag atgccgatca cctcggcgct gttgatcacg ctggggtcgc cggggccgga
      361 ggcgatcgac tccgcgatca gcggcccgcg cacgaagctg cggctgcgga tcaggtacgc
      421 gccggccgcc ggcaggtcct cgcgcgagcc gcagcacagc cgcccgtcgt cgagccactc
      481 ggcgcgcgcg ccgcgctcgc gcgccgccgc aaggatcgcc ttctcctccc agccgatccg
      541 gtcggcgatc acagccaggt cgagcggctc gccgcccgcc tcgggcggcg ggtccggcgc
      601 cagtgaaagc ggcggcgggc tcacagcgcc aggctggtcg ccagcaggtc gtcgacttgc
      661 tcgcggcgca cgaccacgcg gctctcgccg tccttgacga agaccaccgg tgggcgcagg
      721 aagcccatgt agttgctcga catgttgacc gtgtaggcgc cggtcgactg gacggcgagc
      781 acgtcgccgc tgcgcagctc gggcagcatc cggtcgggga agaggatgtc gggctcggag
      841 tggcgcccgc agatcgtcac gcgcatcgcc ggctcgccga acgggtcgcg ggcgagcttg
      901 acctcgtggt gggcgtcgta catcaggtgg cgcgggttgt cggacaggcc gccgtcgacg
      961 atcacgtagt aggcgcgctc gtcgccgggc ggcccggcgg gcttgatcgg gcccaccgtg
     1021 tagagcatca ccccgccttc ggcgacgacc gagcggccgg gctcgatgcc gagccggacg
     1081 tcctcggcgc acatcggcgc gaccttgtcg gcgaacgtgc cggcgatcgc ctcgacccag
     1141 tcctcgaccg gcgccgggtc gtccgagggc cggtagcgca cgccgcgccc gccgccggcg
     1201 acgatccgcc gcgggcggaa gtccggatgg cgctcctgcg cgcgcgccag cacctcggcg
     1261 acgcgctcca tcgcctggcg gtagggcccg aaccgctgct gctgcgagct gaggtggaag
     1321 tggatgccgt cgacgcgcag cgacggctcc gacagcgccc ggtcgatcgc catccccgcg
     1381 aagccgtgct cgatcgacag gccgaacttc gtgtcgtcgc cgacggtgag gaactcgggg
     1441 ttctcgaggc cggtgaccgt cgggttgatc cgcaccagaa ccggttggac cttgcccagc
     1501 cgctcggcga cgcgcgcgat cggcgcgatc tcggacaggt tgtcgatgac gatcacgccg
     1561 cagtcggcgt cgatcgcgaa ctcgaggtcg ggctccttct tgaacgtgcc gtggtaggtg
     1621 atcagctccg gcgggcagcc gcagtgcagc gccatgtgca tctcgccgac cgacaccacc
     1681 tcgagcccga gcccctccga cagcgcgagc tccatcatcg ccggcgcggt gaacgccttg
     1741 caggcgtagg agacgtcgac gtgcggccag cgcgcggtga agccgtccac gtaggcgcgg
     1801 cacatgcggc ggatctccgc ctcgtcgtag aggtagaccg gggtgccgtg ctcctcggcc
     1861 aggtcgagtg tcgagcaccc tccgacccgc aggaccccat cccctccaac ctcctgggtt
     1921 cccagcaaca tccggggtga acgtacacca tcgtcgagcg ctgggtaacc gaatatctcc
     1981 aacttgcacg cccgctggca ggccgctagg atcgccgcca gccgtcaccc ggaggggggt
     2041 ggcgacagat cgctatcgga gggaaggcga gatgaagatc tcagacgcca cgctgcgcga
     2101 taccgcgcac atggccggtg tcgcgttcga cgccgacgac gcccgggtgc tggccggcct
     2161 gctctgcgac gtgggggtcg acatcgtcga ggcggggatc gtctccgacg ccgatcgcag
     2221 cgaggtgccg ctggtccagg cggtcgtcga ggtcgccggc cgcgagcgga ccaagacggt
     2281 gatcctcgcg cgctcgcgca ggcaggtcga ggaggacctc gacgccgcgc tcgagacggg
     2341 cgccggcaac gtgatgctgt cgatcccgac ctcgccgacg cacgcgatgc tgaagctgca
     2401 gaccgacagc gagcggcgga tcatgacgct cgcggcacgg acgatcgagg ccgcgaagag
     2461 cgttggcctg tcggtcacct tcagcgccga ggacggcgcg cgcaccgacc ccggcttcct
     2521 cgagcgctac gtcgccgccg gcgccgaggc cggggctgac cgcttccggc tcgccgagac
     2581 ggtctcgtcg ctggcgccga acgacgtcgg accgctgatc gagcggctcg tcgcggcagc
     2641 cgggccgact gaggtcgaga tgcactcgca caacatgctc gggctctcgg tggcgaacgc
     2701 gctggcagcg gccgatgcgg gcgccgcctg gatctcgtgc acgatcgacg gcctcggcga
     2761 gcgcggcggc aacacgccgc tggccgagat cctctgcgtg ctgcgggtgc tgcgcggcgt
     2821 cgaccgcttc gacctgcgcc cgctctacga cctctcgcgc gaggcggcgg cgcgcgccgg
     2881 gctgcgctgc agcgcgacga ccggcccgac ggccgagctc agctaccagt acgagatccc
     2941 cggccaggtg cgcaacccgg aggcgttcga gctgctgccg ccggaggcgg tcggcaaccg
     3001 ccgcggcgtg cggatcggct cgcggctgaa gccgtcgctg ctcgaggcgt tcctgccaga
     3061 ggagacgctc gacggcgccg acgcggtcga ggtgaccgag cgcatccgcg ccagcgcgcc
     3121 gcacgacaag cacgtctggg atctcgcgac gctcgacgag ctcgcgcgga tccatcgcgg
     3181 cgagctgcgc gccgacgccg ccgacgtccc ggtaacgaac tgaaaacaga aaggcggtaa
     3241 gtcgatgcaa ggtgtatgcc ccgagtgtga gttcgatttc acgacgcccg agatggagac
     3301 gggcgagacg ctcgtctgcc gtgagtgtca gctgacgctc caggtccgcg gccaggaggg
     3361 cgagacgctc gtgctcgagc cggtcgaggc gagcctctct gattggggcg agtgagcgcg
     3421 gcggccgaga acggggcgcc cggcgaggtc gcggcgccgg agccgtccta cgacgcgcgc
     3481 gcggtcgagg cccgtcgcca gcgcgactgg cgcgagcgcg acgcgttcgc aacgccgccg
     3541 cccgaccccg accggcccga cgcctacgtc aaggcggtct gcccgttcac ctcgggccac
     3601 atccacgttg gccacgtgcg cagctacacg atcgccgacg cctacgcacg ctggcggcgg
     3661 atgtccggcg acgccgtgct cttctcgatc ggcttcgacg ccttcgggct gccggcggag
     3721 ctcgccgcta tcgcgaacaa ggagtcgccg gccgactggg tccagcgctc ctacgagcac
     3781 atgctgcgcc agttcgagcg gatgggcttc tctttcgact gggagcgcgt cttcgtcagc
     3841 tcggacgagg agcagtaccg ctggtcgcag tggctcttcc tcgccttcct cgacgccggc
     3901 ctgctctacc aggaggaggc gaccgtcgac tggtgcgaca gctgccagac cacgctcgcc
     3961 tcgctgcagg tcgaggacgg ccgctgctgg cgctgcaacg ggccggcgcg cctgatcaag
     4021 cgcaagcagt ggttcctgcg gatcagcgcc tacctcgagc agaacgagcg cgggatcgag
     4081 gaccacgccg gcaagtggga ccgccccgcc ctgaccgccc agcgcgcgat cctcgggcgt
     4141 gtggacgggg tggagatcga cctgcacggc gcggacggcg cgctgacgtg cttcaccgag
     4201 cacccggacg cgatcgggca ggcgcgcttc gtgctggtct cgccgcgcca ccccgagctc
     4261 gagcggctgc tcggcgaccg gcccggcgtg ctcgccgagc tcgaggaggt gcgccagggc
     4321 ggctggcagc gcggcgaccg ctcggccgag agcgtcccgg cgatcgacag cggcgtccgg
     4381 ctgaccgccc ccaacggcgc cgagctgccg ctggtgatct cgccggcggt cgacggccgc
     4441 tacggcccga ccgcggtgct cggcatcccg gccgccgacg agagcgacgg cgtgatcgcg
     4501 cgccggctcg acctgccgcg ccccgagccg accgcgccca cctcgaacgg caaggcgccc
     4561 cgccccgcgg tccgctaccg cgccggcgac ttcgcgatct cgcgccagcg ctcctggggc
     4621 gcgccgatcc cgatcgtcca ttgcgacgac tgcggcgagg tccccgtgcg caaggaggac
     4681 ctgcccgtcc gcctcccgcg cgacctcaag ccgaccggcg agggcaaccc gctcgccgcg
     4741 cgcgaggatt tcatggcgac gacctgcccg cgctgcggcg ccgacgcgcg ccgcgagacc
     4801 gacacgctcg actgccactt cgacgacctc tggatgtggc tgccgacctg cgtgccgaag
     4861 gccgaccgcg accgccagat gttcgaccac cccgagtgcg agcgctggct gcccggcgcg
     4921 atgctgatcc acggcgccga cggcggcggc tacttcttcg accagaggat caccgccaag
     4981 gcgctgcgcg acatcggccc gctcgcccac ctgaccgacg gcgagcagtt cacgggtgcg
     5041 gtgatgcacg agatggtcac ccaggacggc cgcaagatga gcaagcacct tggcaacgtc
     5101 gtcgaccccg acgacctgat ggagcgcgtt ggcgccgaca ccacacggct cgcggtgctg
     5161 cacggtgccc ggccctcgcg cgcgttgaac tggaccgaga acgcggtcca gtactgcgcc
     5221 aagttcctct ccgacctctg ggcctacgcc gccgagcgcc tgccggggcc cgacccccac
     5281 gaggccgccg ccgcgcccga ccccgacggc gcgccccagc gccgcgagcg cctcgagggc
     5341 tgggtcgcga ccgccgccgc caaggtcacg gaggacctcg agcgcgtcga gatgcagcgc
     5401 gcggtcaaga acgcgatgcg cctcttcgac cgcatcgagg agttcgagac gaagtcccgc
     5461 cgcgccaacc gcggcgacct cgcggc
//
LOCUS       DATABV010000126        17790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1325242, whole genome shotgun sequence.
ACCESSION   DATABV010000126 DATABV010000000
VERSION     DATABV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17790
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325242"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VHF88_05445"
     CDS             complement(<1..411)
                     /locus_tag="VHF88_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161249.1"
                     /translation="MTALRRRLVPYGLLGPGALWLVLFFVVPMYFMGEMALRSGSLDT
                     GGFTLTWEWANFGDALSGSDEQIVRSFVYAGLATVFALAIAYPLAYAIALRAGRWKLL
                     LLFAVVAPFFTTYLIRTIAWKTILADASPAVDVLQ"
     gene            complement(443..1621)
                     /locus_tag="VHF88_05450"
     CDS             complement(443..1621)
                     /locus_tag="VHF88_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2161250.1"
                     /translation="MSNSSDNTRAHRRGPALALLLALVAMLALAACGEDDTVGGGGGG
                     GETEVAQSGPVRGELTISNWPAYMDTGKDNTVAEFEDATGVKVDYVEDVNDNAEFFGK
                     LRPQLERGQSGGRSIFVVTDWMAKQMYDLGYLQELDHADLTTVFDNILPSLESPSFDP
                     ERKFSVPWQSGMTGIMVNEKLAPDIRSVNDLFDPKYKGKVTMLSEMRDTVPLVMKADG
                     VDPADADKQDWLDAIDKLRAAAESGQIRRFTGNDYNQDMTNENVVAAIGWSGDTSIIE
                     NPDVRWRMPAEGCILWSDNMVIPKGAPNTAAALAWMNFVYRPRVQADIAEYVQYVTPV
                     QGVADVLRERDPELADNQLIFPDEEFTADCSTQPDPPGSEEDQQEVTEAFQDMITGQG
                     "
     gene            complement(1605..2717)
                     /locus_tag="VHF88_05455"
     CDS             complement(1605..2717)
                     /locus_tag="VHF88_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161251.1"
                     /translation="MVKRFGGFAAVGGVDLDVESGEFFTLLGPSGCGKTTTLRMIAGF
                     EEPTEGRVLIDGDDVSGLPPYRRPTNTVFQSYALFPHLSVERNVAFGLERKKLAKDEI
                     RRRVGEELERVGLAGEAKRKPRQLSGGQQQRVALARALVNRPAVLLLDEPLGALDLKL
                     RKRLQVELKGIQRDVGITFVYVTHDQEEALTMSDRIAVMNRGLVEQVATPEEVYGRPE
                     TTFVAGFIGVSNLMPGVVEATGPNGRLRLDVGPEVDAVIADGLTVGERCHAVVRPEKL
                     QIADAGAASADGAASVEGEVESSVYLGSATQLVVRLAGDVRMTVLVPNADEALRQRLP
                     GGGARVRLSWAPEHIHVVRESSEHTSLQEERHEQLV"
     gene            2926..4323
                     /locus_tag="VHF88_05460"
     CDS             2926..4323
                     /locus_tag="VHF88_05460"
                     /inference="COORDINATES: protein motif:HMM:NF005102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2161252.1"
                     /translation="MATTPQTVKQDPQGPGSSLQDMAKDHLWMHFTRMSAYSDAEVPI
                     IVRGDGCYVYDEHGKRYLDGLSALFCVNAGHGRTEIGEAMAEQVSELGFYTNWSYAHP
                     RSIELADRIAGYAPEGLDRVFFTSGGSEAVESALKLARIFHRKNGEAGRFKTIAREIA
                     YHGTTMGALGATGIPDLRAPFEPLVPGGRHAPNTNGYRWPEGRDRLWAADQIEERIVF
                     EGPETVSAVILEPVQNAGGCFTPQEGYWQRVREICDRYGVLLISDEVICSWGRLGHWF
                     GCERYDYVPDMITTAKALTSAYAPMGAVIASDRVAAPFLEGTNMFTHGFTFGGHPVCA
                     AAALANLDIFEREDLCGHVLAKEGEFRATLEDLRDIPIVGDVRGAGYFHAIELVKDQE
                     TKETFDDDESEWLLRGFLSGALYENGLICRADDRGDPVIQLSPPLISDSEQFEQIGDV
                     LRRVLTEASDRLVKR"
     gene            4344..5906
                     /locus_tag="VHF88_05465"
     CDS             4344..5906
                     /locus_tag="VHF88_05465"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX2161253.1"
                     /translation="MLTVRDLVDELGLELLSGGDAAEAPVRWVHISELPDPTPWLSGG
                     EMILTTGLQLTTAKRQRQFARLLCDHHLAGVGFGTGFTHEHVPEALLDEARAVGLPVF
                     EIPYELPFIAITEKAFGRLVNEQYEVLQRGIAIHKRLERLVLEERGLDELVGAVSAAI
                     GGIVMVLDARGETLAAKTFRRELGDAAVEALRASVREQGGNRVARDFVPEHPEIAGRS
                     LALPVSTRGRGAPQAWLVASRDAGNLGDFERLILQQAATVVALELMRQRVMRDTERRL
                     AGDVLAQALTGRLAESEIESRLRPFGVGEQAAVLVFATDDPGAAEPDLEAALADADVG
                     ALVACRERLLCAVVDAGGRELDPVDLARSMRAALLAGHGGDHVRAAASRVASVGLVRR
                     SFHEARCALEATGFANGAAPDVASHEDLGAFRLLLAVQDDDALRVYTESVLGPLEDGG
                     GEYGDELLRSLEAFIEQNGQWEKAARQLYCHRHTLRYRIKRIEDLTGRDLKSARDRIE
                     LWLALRARELIA"
     gene            5960..7096
                     /locus_tag="VHF88_05470"
     CDS             5960..7096
                     /locus_tag="VHF88_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX2161254.1"
                     /translation="MRVGVLGAGGIIAPAIVRDLAESEEVGDLLLLDLDLERAERVAV
                     EHGGPRARAAEVDARGGLADALGDVDVLVNAASYRVNLDAMRACLQAGCHYVDLGGLY
                     WMTGNQLELSGDFEQAGLLALLGMGSSPGKTNVMAARAVRELGEAPRRIDVIAAGRDL
                     DPPDGASFPYALQTLVDELTMEPMAQQSGRPTALAPMQPGGAVDLPAPIGRAETIYTL
                     HSEVRTFPSSFGCTECSFRLSLHPATLERVRALAEADELEIAAASQAAMPASPETISI
                     HRVIAEGPGGDAVTVTATTIPTEEWGIGGSIVSTAAPAAAAVRLLARDRIARRGALPP
                     ELCVEADDLFPELERRGCAFEVEARRAAGDRADEAGSSEAEAVG"
     gene            7093..8223
                     /gene="ald"
                     /locus_tag="VHF88_05475"
     CDS             7093..8223
                     /gene="ald"
                     /locus_tag="VHF88_05475"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX2161255.1"
                     /translation="MRVGVPTEVKPDEYRVALTPAGVRELGEHGHEVIVQAGAGEGSG
                     IADADYAAQGARIAPDAAAVFGDAELVLGVKEPQPEEVALLRPEHTLFTYLHLAPAPE
                     LTRGLCESGATCVAYETVEDAHGRLPLLAPMSEVAGKIATQAGAFMLERPLGGRGVLL
                     GGVPGVAAANVMVIGGGVVGFNAAKIAIGMDADVFVYDRDVDRLRELDVAFAGRASTV
                     YSSTLAVEEMLARADLVIGAVLVHGARAPYVIKREQLALMKPRSVLVDVSIDQGGCFE
                     TSRPTTHSDPTYEVDGITHYAVTNMPGAVPITSTYALTNATLPYVLALADHGLKGAID
                     FHPGLAPGVNVIGGRVTLAAVAEGTGLEHTPLEEVLGELEPA"
     gene            8267..9706
                     /locus_tag="VHF88_05480"
     CDS             8267..9706
                     /locus_tag="VHF88_05480"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX2161256.1"
                     /translation="MATTTVKLKNFIGGELVEPAAGQTEEVLDPATLEVIGEAPLSSE
                     EDVNRAVAAARKAFDGWWATTPGERANCLYKLAEVFEERAEEIADLESADAGKPRHEV
                     DGEVESMVDNLRFFAGAGRTMGGLAAGEYMDGKTSIIRREPVGVVGQITPWNYPLMMA
                     VWKIGPALATGNTVVLKPAETTPVTTLKMAEWAADVFPPGVLNVIGGHGEAAGAPLVR
                     HPDVDMVSLTGSPPTGKWIAAAAAETLKKVHLELGGKAPVVVFDDVDMETALATIAEA
                     GYYNAGQDCTAATRVLASKRVYDDVVSGLASEAGKQVVGLTRSEDTTLGPLNSERQRE
                     RVESFLERKRDGVEIVTGGGRSDGLPGYYLEPAVVAGVSQDDELAQKEIFGPVITVQP
                     FDDEAKAIEWANGTRYGLSASVWTRDVGRAMRVSRALRFGCVWINDHFSITPEMPHGG
                     YKESGYGKDMSMYSLEDYTQVKHIVASLD"
     gene            complement(9703..11313)
                     /locus_tag="VHF88_05485"
     CDS             complement(9703..11313)
                     /locus_tag="VHF88_05485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2161257.1"
                     /translation="MSGPNRTRLVLFAAVVVVATAVWAVSETQRATAERIYKESQAAD
                     RMLTAMLDQETGLRGFVITRERPFLQPFIDGVRNFNLAADAARSYADDDTRPAIDRME
                     ATANRWRWSAEQEVGRVGLQRNARFDRASTLRRKALFDSFRRQSTDLAERLAKERSDK
                     LERAGLISVAVIVVLSLLFGGIGFALLERQVRRSRARRAAERSYRRTQSEFIETMQIM
                     RDEPEAHALVKQHLERSIDGSEALVLTRNNSDNRLAAATTVTADSVIAERLADAEPES
                     CLAVRLGREYRQHPGDSPLLTCDLCGASAEEVVCTPSLVGGEVIGSVLVRSNEPLQRE
                     DRQRIEDSVSQAAPVLANLRNLAIAEARAATDALTGLPNSRSCRDNLKRMVAHAGRTV
                     SPLSAAMFDLDHFKRVNDRFGHGAGDDVLAAVGEILRSTLRDSDFGGRYGGEEFLLLL
                     PSTDQQGALDVTEKLRAAIEALEFQQPELTVTASFGIATYPLDALDADSLVRMADRAL
                     YAAKARGRNRIELLDSSQPTPEREPQDA"
     gene            11392..11865
                     /locus_tag="VHF88_05490"
     CDS             11392..11865
                     /locus_tag="VHF88_05490"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2161258.1"
                     /translation="MASYAFLTTWLLRAPRTVVWDAIQDTAAWPEWWRGVESVEELDG
                     GDGQRVGSRYRVRWRSFVPYSVAFDFEVERVELPVLMSGRATGDLEGTGTWRLYEQDG
                     VTAVTYDWRVRTTKPWMNLVAPLARLVFEWNHDWVMARGGEGLAARLGVELVAAG"
     gene            complement(11887..12555)
                     /locus_tag="VHF88_05495"
     CDS             complement(11887..12555)
                     /locus_tag="VHF88_05495"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX2161259.1"
                     /translation="MSRRFALLFATVLLALAAGCSSDDDNGGNRPSSEDVPSGAVAKV
                     GDVEISEADLDRQVEALARAQRGSGSAASDGSAPGGASSGDADGGPSAEEQLREQLEA
                     QALATLLQRQALEQEAKDRGIEVTDAEVRQRWEAASRGQFKTKKALRRFLGGQTEQDL
                     LDQLRLQVLTERIHEQVSEEAGGGKQGAKAVARFQKEFQQRWQDRTACREGFNAPGCT
                     DDSK"
     gene            complement(12558..13259)
                     /locus_tag="VHF88_05500"
     CDS             complement(12558..13259)
                     /locus_tag="VHF88_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161260.1"
                     /translation="MELGTSHMRRFIRLLAVVGALSLAGAGLAACGGDDDEPQSSPRA
                     AEQTETTKPKASGGKREGGGSKADKRDGGQRDSGPALTGDREEAREGVEAVDEVYEGF
                     DAAAEAGVAEVDVPAIQTLETADDNESLTNVCDLMSEKAKRQTIVYAKRSAGLADVEW
                     TCEKATGLLLRRARQTGGLKRSLRAEVVGVNVEGDRATASIRFGGKGPISSVPLVKED
                     GEWKLAASPSGGGGQ"
     gene            complement(13364..13810)
                     /locus_tag="VHF88_05505"
     CDS             complement(13364..13810)
                     /locus_tag="VHF88_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161261.1"
                     /translation="MTDHESRRRLAGAVAATLAVALGAFAGSACGAGDEATDPKGPVD
                     GTGPAAKQIRATYAAFTDAVYEGDFEAACATMTRTLRRSLDEGRRCSNGLRAVYGDGA
                     FDRTRPRIARLRIDGDSAEAEVQPGAGGASTKLTFVRREGEWRMAR"
     gene            complement(13877..14590)
                     /locus_tag="VHF88_05510"
     CDS             complement(13877..14590)
                     /locus_tag="VHF88_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161262.1"
                     /translation="MRRLTLMTTMAVLTLAVAAGSAMAQTLRPPEPPHPAMAADWKAV
                     NAVDWSSTPSKRYLCGHPDAVCEFSDNAVLTTSFTGFGIHTIGSCSATLSGDVSSDGD
                     VTITGVTPTDCGLGTVDPAKLPATGEICMHKPTQELWVRQHLTNDGPPLYAQVLVQPS
                     GLATDRPTFFARGIMIGNPSVPTFATHTYAAGSAGPSFNNFKHAAFDAMWATSGLNAH
                     KLLLGQNVEQPACGWAELS"
     gene            complement(14696..>17790)
                     /locus_tag="VHF88_05515"
     CDS             complement(14696..>17790)
                     /locus_tag="VHF88_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161263.1"
                     /translation="ATVWAPEPVPSGSPPTGQNVAVPAPATPDQCSDWGRLAPHGSPP
                     PDRPIWRAPPSWRLAPVTQAGAHPDGSATFWLARNSDRLVDGTLDNVYGELPAGFVGD
                     PTAVPKCTAEQFAVRPLQCPPETQVGVLHLYIVGAGAGGGVNYQASTEEILPVYNLEP
                     RKGNVAELGVAYVSGEDATTARIVAKARTNGDFGVTTFVSQLPAALPVIGQSVTLWGV
                     PWLSSHDVWRPPTAWRPASSSPNRFGEIPPQGLDPDDRVAYRPAWGEIKPFLSNPTEC
                     SGATLETRLAIDSYEAPGSFTPEGDPDPLDVNWKRYASPAPRVTGCDKVPFAADLAVQ
                     PTSTVADSASGLIAGLDLPQNDDLPFAAPAAGAGEAEVDAYVDAAAGHWASDAGLATS
                     QLDRAVVSLPAGVSVNPAGAAGLEGCSDAGIGVTDSGTNPMRFNNGDPFDKDGGADGA
                     ECPAGSKIGTVEVDTPLLDEPLTGEVVLGVPKSTDPTSGEMFRMFVVVRSVERGLIAK
                     IYGSAVADPQTGRLTATFDKNPRVPFETMRMRLKGGDRGLLALPPRCQASGWTSEFSP
                     WTAAHDAGGLPVSDGGAFVTDQRCGFGFAPGLRAGMNGRQGGGSGAFSFVLTREEGQQ
                     WLAGVSVSMPGGLLAAVRDVALCSNAQAAANACPAASRIGMADAGAGSGAPFFLERKG
                     DVFLTEGYKGAPYGLAVSVPVEAGPFRGPLALSPIVVRQALHVDPSTAAVTAVSDPLP
                     QIHHGIPLRVRQIIVTVDRPGFMRNPTDCSPKRIAADVTSVEGAVARPASPYQATGCA
                     ALPFRPKLALRLTGRKQMRTGRHPGVRARVTQRSGEAGIEKARVRLPKTLALDPENAR
                     ALCEYEDGVRPDLERHCPKGSIVGRARAVSPLLKGPLSGNVYFVKNVRRSSSGNLIRT
                     LPMIVVALRGEVAINLRGESSVDRRNRLVNTFASVPDAPISRFDLRIAGGRSGILAVT
                     RTRRSRIDLCSRRQVAAVDMDGHNGRRRDGRTRVSTPCKASKKKRRADRRKRSVKRRR
                     "
BASE COUNT         2472 a         6339 c         6438 g         2541 t
ORIGIN      
        1 ctgcaggacg tcgaccgccg gactggcgtc ggcgaggatc gtcttccagg cgatcgtgcg
       61 gatcaggtac gtcgtgaaga acggggcgac gaccgcgaac agcagcagca gcttccagcg
      121 accggcgcgc agcgcgatcg cgtaggcgag cgggtaggcg atcgccagcg cgaagaccgt
      181 cgccagcccc gcgtagacga acgagcgcac gatctgctcg tcgctgcccg agagcgcgtc
      241 gccgaagttc gcccactccc aggtcagcgt gaagccgccg gtgtccagcg accccgaccg
      301 cagcgccatc tcgcccatga agtacatggg cacgacgaag aacaggacca gccagagcgc
      361 ccccgggccg agcaggccgt aggggacgag ccggcggcgc agcgcggtca cgagagcgcc
      421 tgtccgccgc cggccgccgt cactaaccct ggccggtgat catgtcctgg aatgcctccg
      481 tgacctcctg ctggtcctcc tcggagccgg gcgggtccgg ctgggtcgag cagtcggcgg
      541 tgaactcctc gtccgggaag atcagctggt tgtcggccag ctcggggtcg cgctcgcgca
      601 ggacgtcggc gacgccctgg accggcgtca cgtactggac gtactcggcg atgtctgcct
      661 gcacccgcgg ccggtagacg aagttcatcc acgcgagggc ggcggccgtg ttcggcgccc
      721 ccttcgggat caccatgttg tccgaccaga ggatgcagcc ctcggccggc atccgccacc
      781 tgacatcggg gttctcgatg atcgacgtgt cgcccgacca gccgatcgcc gcgacgacgt
      841 tctcgttggt catgtcctgg ttgtagtcgt tgccggtgaa gcggcgaatc tgccccgact
      901 cggccgccgc gcgcagcttg tcgatcgcgt cgagccagtc ctgcttgtcg gcgtcggcgg
      961 ggtcgactcc gtcggccttc atgaccagcg gcacggtgtc gcgcatctcg gagagcatcg
     1021 tcaccttgcc cttgtacttc gggtcgaaga ggtcgttgac ggacctgatg tcgggcgcga
     1081 gcttctcgtt gaccatgatc ccggtcatcc cgctctgcca cggcaccgag aacttgcgct
     1141 cggggtcgaa cgacgggctc tcgaggctcg ggaggatgtt gtcgaagacc gtcgtcaggt
     1201 cggcgtggtc gagctcctgg aggtagccga ggtcgtacat ctgcttcgcc atccagtcgg
     1261 tcacgacgaa gatgctgcgg ccgcccgact ggccgcgctc gagctgcggg cgcagcttgc
     1321 cgaagaactc ggcgttgtcg ttgacgtcct cgacgtagtc gaccttgacg ccggtcgcgt
     1381 cctcgaactc ggcgacggtg ttgtccttgc cggtgtccat gtaggccggc cagttggaga
     1441 tcgtcagctc gccgcggacc gggcccgact gcgcgacctc cgtctcaccg ccgccgccac
     1501 cgccgccgac ggtgtcgtcc tcgccgcagg cggcgagcgc gagcatcgcc acgagcgcca
     1561 gcagcagcgc gagcgccggc ccccgccgat gtgctctggt gttgtcagac gagttgctca
     1621 tggcgttcct cctgaaggga tgtgtgctcc gacgactcgc gcacgacatg gatgtgctcg
     1681 ggcgcccagc tcagccgtac ccgcgcaccg cctccgggca accgctgccg cagcgcctcg
     1741 tctgcgttgg ggacgagcac ggtcatccgc acgtcgcccg cgagccgcac gacgagctgg
     1801 gtggcgctgc cgaggtagac cgagctctcg acctcgccct cgacgctggc ggcgccgtcc
     1861 gcactggccg ccccggcgtc cgcgatctga agcttctccg gacgcacgac cgcgtggcag
     1921 cgctcgccga ccgtcagccc gtcggcgatc acggcgtcga cctccggccc gacgtcgagc
     1981 cgcaggcggc cgttggggcc ggttgcctcg acaacgccgg gcatcaggtt cgagacgccg
     2041 atgaagccgg cgacgaaggt tgtctccggc cgcccgtaga cctcctccgg ggtcgcgacc
     2101 tgctcgacca gcccgcggtt catcaccgcg atccggtccg acatcgtcag cgcctcctcc
     2161 tggtcgtggg tgacgtagac gaaggtgatg ccgacgtcgc gttggatgcc cttgagctcc
     2221 acctggaggc gcttgcgcag cttgaggtcg agtgcgccga gcggctcgtc gagcagcagc
     2281 accgccgggc ggttgacgag cgcccgcgcg agcgcgacgc gctgctgctg gccgcccgac
     2341 agctggcgcg gcttgcgctt ggcctcgccg gcaaggccga cgcgctcgag ctcctcgccg
     2401 acgcggcggc ggatctcgtc cttggcgagc ttcttgcgct cgagcccgaa ggcgacgttg
     2461 cgctcgacgc tcaggtgcgg gaacagcgcg tagctctgga agacggtgtt ggtcgggcgg
     2521 cggtagggcg gcaggccgga gacgtcgtcg ccgtcgatca gcacgcggcc ctcggtcggc
     2581 tcctcgaagc ccgcgatcat ccgcagcgtc gtcgtcttgc cgcagccgct agggccgagc
     2641 agcgtgaaga actcgccgct ctcgacgtcg aggtcgacgc cgccaacggc cgcaaagccg
     2701 ccgaagcgct tgaccaccct ctccagtctg actccgccgc ggctcattcc cctcccacgt
     2761 tatgtccgtt cggtagccgg agcaaccggc gcgcggccca aaccgaacgg cgcgagttct
     2821 cccaactgta caagccggtg tcgagtggat ttcacgttcg gtgggttgtt cgtcggctgt
     2881 agcccgggta ccgttgtcca cagcgtctag tctcgaggga gaacagtggc gacaacaccg
     2941 caaaccgtca agcaggatcc gcagggcccg ggcagctcgc tgcaggacat ggcgaaggac
     3001 cacctgtgga tgcacttcac gcggatgagc gcgtacagcg acgccgaggt gccgatcatc
     3061 gtgcgcggcg acggctgcta cgtctacgac gagcacggca agcgctacct cgacgggctc
     3121 tcggcgctgt tctgcgtcaa cgccggccac ggccggaccg agatcggcga ggcgatggcc
     3181 gagcaggtct ccgagctcgg cttctacacc aactggagct acgcccaccc gcgctcgatc
     3241 gagctcgccg accggatcgc cggctacgcg ccggagggtc tcgaccgcgt cttcttcacc
     3301 tcgggcggca gcgaggcggt cgagtccgcg ctcaagctcg cgcggatctt ccaccgcaag
     3361 aacggcgagg ccggccgctt caagacgatc gcgcgcgaga tcgcctacca cggcacgacg
     3421 atgggcgcgc tcggcgcgac cgggatcccc gacctgcgcg cgccgttcga gccgctcgtg
     3481 ccgggcgggc gccacgcgcc gaacaccaac ggctaccgct ggcccgaggg ccgcgaccgg
     3541 ctctgggcgg ccgaccagat cgaggagcgg atcgtcttcg aggggcccga gacggtgtcg
     3601 gccgtgatcc tcgagccggt ccagaacgcg ggcggctgct tcacgcccca ggagggctac
     3661 tggcagcgcg tgcgcgagat ctgcgaccgc tacggcgtgc tgctgatctc cgacgaggtg
     3721 atctgctcgt ggggccggct cggccactgg ttcggctgcg agcgctacga ctacgtgccg
     3781 gacatgatca cgacggcgaa ggcgctgacc tccgcctacg cgccgatggg cgcggtgatc
     3841 gcgtcggacc gcgtcgcggc gccgttcctc gagggcacga acatgttcac gcacgggttc
     3901 accttcggcg gccatcccgt ctgcgcggcg gcggcgctgg cgaacctcga catcttcgag
     3961 cgcgaggacc tctgcgggca cgtgctcgcc aaggagggcg agttccgcgc gacgctcgag
     4021 gacctgcgcg acatcccgat cgtcggcgac gtgcgcgggg ccggctactt ccacgcgatc
     4081 gagctggtca aggaccagga gaccaaggag acgttcgacg acgacgagtc ggagtggctg
     4141 ctgcgcggct tcctctcggg cgcgctctat gagaacgggc tgatctgccg cgccgacgac
     4201 cgcggcgacc cggtgatcca gctctcgccg ccgctgatct ccgacagcga gcagttcgag
     4261 cagatcggcg acgtgctgcg cagggtcctg accgaggcct cggaccgcct ggtgaagcga
     4321 tagagcgcca tggcgcacct ggcatgctga ccgtccgcga cctggtcgac gagctcggtc
     4381 tcgagctgct cagcggcggt gacgcggccg aggcgccggt gcgctgggtg cacatcagcg
     4441 agctgcccga cccgacgccg tggctgtcgg gcggcgagat gatcctgacg accggccttc
     4501 agctgacgac cgccaagcgc cagcgccagt tcgcgcgcct gctctgcgac caccacctcg
     4561 ccggcgtcgg cttcggcacc ggcttcacgc acgagcacgt gcccgaggcg ctgctcgacg
     4621 aggcgcgcgc ggtcggcctg ccggtcttcg agatccccta cgagctgccg ttcatcgcga
     4681 tcaccgagaa ggcgttcggg cggctggtca acgagcagta cgaggtgctg cagcgcggca
     4741 tcgcgatcca caagcggctc gagcggctgg tgctcgagga gcgcgggctc gacgagctgg
     4801 tcggcgcggt gtcggcggcg atcggcggga tcgtgatggt gctcgacgcg cgcggcgaga
     4861 cgctcgcggc gaagacgttc cggcgcgagc tgggcgacgc cgccgtcgag gcgctgcggg
     4921 cgagcgtgcg cgagcagggc ggcaaccgcg tggcgcgcga cttcgtgccc gagcacccgg
     4981 agatcgcggg ccgctcgctc gcgctgccgg tctcgacacg cggacggggg gcgccccagg
     5041 cgtggctggt cgccagccgc gacgccggca acctcggcga cttcgagcgg ctgatcctgc
     5101 agcaggcggc gacggtcgtc gcgctcgagc tgatgcgcca gcgcgtgatg cgcgacaccg
     5161 agcgccggct ggccggcgac gtgctcgccc aggcgctgac cggccgcctg gccgagagcg
     5221 agatcgagtc gcggctgcgt cccttcggcg tcggcgagca ggcggcggtg ctggtcttcg
     5281 cgaccgacga ccccggcgcc gccgagccgg atctcgaggc cgcgctcgcg gacgccgacg
     5341 tcggcgcgct ggtcgcctgc cgcgagcggc tgctctgcgc ggtcgtcgac gccggcggcc
     5401 gtgagctcga cccggtcgac ctcgcgcgct cgatgcgcgc ggcgctgctc gccgggcacg
     5461 ggggcgatca cgtgcgcgcc gccgcgagcc gcgtggcctc cgtcgggctc gtgcggcgca
     5521 gcttccacga ggcgcgctgc gcgctcgagg cgaccgggtt cgccaacgga gcggcgcccg
     5581 acgtcgcctc gcacgaggac ctcggcgcct tccggctgct gctcgccgtc caggacgacg
     5641 acgcgctgcg cgtatacacc gagagcgtgc tcgggccgct cgaggacggc ggcggcgagt
     5701 acggcgacga gctgctgcgc tcgctcgagg cgttcatcga gcagaatggc cagtgggaga
     5761 aggcggcgcg gcagctctac tgccaccgcc acacgctgcg ctatcggatc aagcggatcg
     5821 aggatctgac cgggcgcgac ctgaagtcgg cgcgcgaccg gatcgagctg tggctggcat
     5881 tgcgggcacg ggagctgatc gcatgatgag gacgaggggc tcacgccccc cgagcccccc
     5941 gcgggaccct ggccgcgcga tgcgagtcgg cgtgttgggt gctggaggga tcatcgctcc
     6001 cgcgatcgtg cgcgacctcg ccgagtcgga ggaggtcggc gacctgctgc tgctcgacct
     6061 cgacctcgag cgcgccgagc gggtcgccgt cgagcacggc gggccgcggg cgcgggcggc
     6121 ggaggtcgat gcgcgcggcg ggctcgccga cgcgctcggc gacgtcgacg tcctggtcaa
     6181 cgcggcgagc taccgcgtca acctcgacgc gatgcgcgct tgcctgcagg ccggctgcca
     6241 ctacgtcgac ctcggcggcc tctactggat gaccggcaac cagctcgagc tgtccggcga
     6301 cttcgagcag gcggggttgc tggcgctgct cggcatgggg tcgagcccgg gcaagacgaa
     6361 cgtgatggcc gcgcgcgcgg tgcgcgagct cggcgaggcg ccgcgccgga tcgacgtgat
     6421 cgccgccggc cgcgacctcg acccgcccga cggggcgagc ttcccctacg cgctgcagac
     6481 gctcgtcgac gagctgacga tggagccgat ggcccagcag agcggacggc cgacggcgct
     6541 cgcgccgatg cagcccggcg gggccgtcga cctgcccgcg ccgatcggcc gcgccgagac
     6601 gatctacacg ctgcactccg aggtgcgcac cttcccgtcg agcttcggct gcaccgagtg
     6661 cagcttccgc ctgtcgctgc acccggcgac gctcgagcgc gtgcgcgcgc tcgccgaggc
     6721 ggacgagctc gagatcgcgg ccgcatcgca ggcggcgatg ccggcctcgc cggagacgat
     6781 ctcgatccac cgtgtgatcg ccgagggccc cggcggcgac gcggtgacgg tgacggcgac
     6841 gacgatcccg accgaggagt ggggcatcgg cggcagcatc gtctcgaccg cggcgcccgc
     6901 cgcggccgcg gtgcgcctgc tggcgcgcga ccggatcgcg cgccgcggcg cgctgccgcc
     6961 cgagctctgc gtcgaggccg acgacctgtt ccccgagctc gagcggcgcg gctgcgcctt
     7021 cgaggtcgag gcccggcgcg ccgccggcga tcgagccgac gaggcaggca gctcggaggc
     7081 ggaggcggtc ggatgagggt cggggtccct accgaggtca agcccgacga gtaccgggtg
     7141 gcgctgacac cggccggcgt ccgcgagctc ggcgagcacg gccacgaggt gatcgtccag
     7201 gctggcgccg gcgagggctc cggcatcgcc gacgccgact acgccgccca gggcgcgcgg
     7261 atcgcgcccg acgccgcggc ggtcttcggc gacgccgagc tggtgctcgg cgtcaaggag
     7321 ccacagccgg aggaggtcgc gctgctgcgg cccgagcaca cgctgttcac ctacctccac
     7381 ctggcgccgg cgcccgagct gacgcgcggc ctctgcgagt cgggcgccac ctgcgtcgcc
     7441 tacgagaccg tcgaggacgc ccacggccgg ctgccgctgc tggcgccgat gagcgaggtc
     7501 gccggcaaga tcgcgaccca ggccggcgcc ttcatgctcg agcgcccgct cggcgggcgc
     7561 ggcgtgctgc tcggaggcgt gcccggcgta gcggcggcga acgtgatggt gatcggcggc
     7621 ggggtggtgg gcttcaacgc cgcgaagatc gcgatcggca tggacgccga tgtgttcgtc
     7681 tacgaccgcg acgtcgaccg cctgcgcgag ctcgacgtcg ccttcgcggg gcgcgcgtcg
     7741 accgtctact catcgacgct cgcggtcgag gagatgctgg cgcgcgcgga tctcgtgatc
     7801 ggcgcggtgc tggtccacgg cgcccgcgct ccctacgtga tcaagcgcga gcagctcgcg
     7861 ctgatgaagc cgcgctcggt gctcgtcgac gtctcgatcg accagggcgg ctgcttcgag
     7921 acgtcgcgac cgaccaccca ctccgacccg acctacgagg tcgacgggat cacccactac
     7981 gccgtcacca acatgcccgg cgcggtgccg atcacctcga cctacgcgct gaccaacgcg
     8041 acgctgccct acgtgctcgc actggccgac cacgggctca agggcgcgat cgatttccac
     8101 cccggactgg ccccgggcgt taacgtgatc ggcgggcgag tgacactcgc cgccgtggcg
     8161 gagggcacgg gactcgagca cacgccgctc gaggaggttc tcggtgagct ggagccggcc
     8221 taggccttcc acgaaaacga cgacgaggca ctgcgaggga acgaagatgg caactaccac
     8281 cgtcaagctg aagaacttca tcggcggcga gctcgtcgag ccggccgccg gacagaccga
     8341 ggaggtgctc gacccggcga cgctcgaggt gatcggcgag gcgccgctct cgagcgagga
     8401 ggacgtcaac cgtgcggtgg ccgccgcgcg caaggcgttc gacggctggt gggcgacgac
     8461 gcccggcgag cgggcgaact gcctttacaa gctggccgag gtcttcgagg agcgcgccga
     8521 ggagatcgcc gacctcgagt ccgccgacgc cggcaagccg cgccacgagg tcgacggcga
     8581 ggtcgagtcg atggtcgaca acctgcgctt cttcgccggc gccggtcgca cgatgggcgg
     8641 gctcgcggcg ggcgagtaca tggacggcaa gacgtcgatc atccgccgcg agccggtcgg
     8701 cgtcgtcggc cagatcacgc cctggaacta cccgctgatg atggccgtct ggaagatcgg
     8761 cccggcgctc gcgaccggca acacggtcgt gctcaagccg gccgagacga cgccggtgac
     8821 gacgctgaag atggccgagt gggcggccga cgtcttcccg ccgggcgtgc tgaacgtgat
     8881 cggcggccac ggcgaggccg ccggcgcgcc cctggtgcgg caccccgacg tcgacatggt
     8941 ctcgctgacc ggctcgccgc cgacggggaa gtggatcgcg gctgcggccg ccgagacgct
     9001 gaagaaggtc cacctcgagc tcggcggcaa ggcgccggtg gtggtcttcg acgacgtcga
     9061 catggagacc gcgctggcga cgatcgccga ggccggctac tacaacgcgg gccaggactg
     9121 cacggcggcg acgcgcgtgc tggcgtcgaa gcgcgtctac gacgacgtcg tcagcgggct
     9181 cgcgtcggag gccggcaagc aggtcgtcgg gctgacgcgg tcggaggaca cgacgctcgg
     9241 gccgctgaac agcgagcgcc agcgcgagcg cgtcgagagc ttcctcgagc gcaagcgcga
     9301 cggcgtcgag atcgtgaccg gcggcgggcg cagcgacggc ctgccgggct actacctcga
     9361 gccggccgtg gtcgccggcg tctcccagga cgacgagctc gcgcagaagg agatcttcgg
     9421 gccggtgatc accgtccagc cgttcgacga cgaggcgaag gcgatcgagt gggccaacgg
     9481 cacccgctac ggcctgtcgg cgtcggtctg gacccgcgac gtcggccgcg ccatgcgcgt
     9541 ctcgcgcgcg ctgcgcttcg gctgcgtatg gatcaacgac cacttctcga tcacccccga
     9601 gatgccccac ggcggctaca aggagtccgg ctacggcaag gacatgtcga tgtactcgct
     9661 ggaggactac acgcaggtca agcacatagt cgccagcctc gactaagcgt cctggggctc
     9721 acgctcgggc gtgggctggg aggagtcgag cagctcgatg cggttgcggc ccctggcctt
     9781 ggcggcgtag agggcgcggt cggccatccg gacgaggctg tcggcgtcga gcgcgtcgag
     9841 cgggtaggtg gcgatgccga agctggccgt gaccgtcagc tccggctgct ggaactcgag
     9901 cgcctcgatc gcggcgcgca gcttctcggt gacgtcgagg gcgccctgct ggtcggtgct
     9961 cgggagcagc agcaggaact cctcgccgcc gtagcggccg ccgaagtcgc tgtcgcgcag
    10021 cgtggagcgc aggatctcgc cgacggcggc gagcacgtcg tcgccggcgc cgtggccgaa
    10081 gcggtcgttg acgcgcttga agtggtcgag gtcgaacatc gcggcggaca gcggcgagac
    10141 cgtgcggccg gcatgggcga ccatccgctt gaggttgtcg cggcaggagc gcgagttcgg
    10201 caggcccgtg agcgcgtcgg tcgccgcgcg cgcctcggcg atcgcgaggt tgcgcaggtt
    10261 cgcgagcacg ggcgcagcct gggacaccga atcctcgatc cgctggcggt cctcgcgctg
    10321 gagcggctcg ttcgagcgga cgagcaccga gccgatcacc tcgccgccga ccagcgacgg
    10381 cgtgcagacg acctcctccg ccgaggcgcc gcagaggtcg caggtcagca gcggggagtc
    10441 gccgggatgc tggcggtact cgcgcccgag gcggaccgcc aggcacgact ccggctcggc
    10501 gtcggccagc cgctcggcga tcaccgagtc ggcggtcacg gtcgtcgccg cggcgagacg
    10561 gttgtcggag ttgttgcgcg tcagaaccag cgcctcgctg ccgtcgatcg agcgctcgag
    10621 atgctgcttg acgagcgcgt gcgcctcggg ctcgtcgcgc atgatctgca tcgtctcgat
    10681 gaactcggac tgggtgcggc gatagctgcg ctcggccgcg cgccgcgcac gcgagcggcg
    10741 gacctgccgc tcgagcaggg cgaagccgat cccgccgaag agcaggctca gcaccacgat
    10801 caccgccacc gagatcagcc ccgcccgctc gagcttgtcg ctgcgctcct tggcgagccg
    10861 ctcggcgagg tcggtgctct ggcgccggaa gctgtcgaag agcgccttgc gccggagcgt
    10921 ggacgcgcga tcgaagcggg cgttgcgctg gaggcccacc cgtccgacct cctgctcggc
    10981 gctccagcgc cagcggttcg cggtcgcttc catgcggtcg atcgcgggac gcgtgtcgtc
    11041 gtcggcgtag gagcgggcgg cgtcggccgc gaggttgaag ttgcgcacgc cgtcgatgaa
    11101 cggctgcagg aacgggcgct cgcgcgtgat cacgaagccg cgcaggccgg tctcctggtc
    11161 gagcatcgcc gtcagcatgc ggtcggccgc ctggctctcc ttgtagatcc gctcggcggt
    11221 cgcgcgctgg gtctccgaca ccgcccagac ggcggtggcg acgacgacga ccgcggcgaa
    11281 caggacgaga cgggtgcggt tggggccgga catccgctgc ttcgcatcgg ccagttggcc
    11341 gtaggacttg agccgatggc gcgttggcta ccggctccct aacctgctcc cgtggccagc
    11401 tacgccttcc tgacgacctg gctgctgcgc gccccgcgga cggtcgtctg ggacgcgatc
    11461 caggacaccg cggcgtggcc ggagtggtgg cgcggcgtcg agtcggtcga ggagctcgac
    11521 ggcggcgacg ggcaacgcgt cggcagccgc taccgcgtgc gctggcgcag cttcgtcccg
    11581 tactcggtcg cgttcgactt cgaggtcgag cgggtcgagc tgccggtcct gatgagtggc
    11641 cgcgcgacag gcgacctcga ggggacgggg acctggaggc tgtacgagca ggacggcgtg
    11701 accgcggtca cctacgactg gcgcgtgcgg accaccaagc cttggatgaa cctcgtcgcg
    11761 ccgctggcgc gcctggtgtt cgagtggaac cacgactggg tcatggcgcg cggcggggag
    11821 ggcctggcgg cgcggctggg cgtggagctg gtcgcggcgg gctaaccgcc ccgcggcgct
    11881 tggacgctac ttcgaatcgt ccgtgcaacc gggggcattg aagccctcac gacaggcggt
    11941 gcggtcctgc cagcgctgct ggaactcctt ctggaaccgc gcgacggcct tggcgccctg
    12001 cttgccgccg cccgcctcct cgctcacctg ctcgtggatc cgctcggtga gcacctgcag
    12061 tcggagctgg tcgaggaggt cctgctcggt ctgcccgccg aggaagcgcc gcagcgcctt
    12121 cttcgtcttg aactgccctc gactggcggc ctcccagcgc tgacgcacct cggcgtcggt
    12181 gacctcgatg ccgcggtcct tcgcctcctg ctcgagcgct tggcgctgga gcagcgtggc
    12241 aagcgcttga gcctcgagct gctcgcgcag ctgctcctcc gcgctgggcc cgccgtcggc
    12301 gtcgccggac gaagccccgc ccggcgccga gccgtcggat gcggcggagc cactgccgcg
    12361 ctgggcgcgc gcgagcgcct cgacctgccg gtccaggtcg gcctcgctga tctctacgtc
    12421 gccgaccttc gcgacggcgc cggagggaac gtcctccgag ctcgggcggt tgccgccgtt
    12481 gtcgtcgtcg ctgctgcaac cggcggcgag cgccaacagg accgtggcga agagcagcgc
    12541 gaaccggcgc gacatggcta ctggccgcct ccgcccgagg gagaggccgc gagcttccac
    12601 tcgccgtcct ccttgaccag cggcacggac gagatcggcc ccttgccgcc gaagcggatc
    12661 gaggccgtcg cccgatcgcc ctcgacgttg actccgacca cctccgcgcg cagggagcgc
    12721 ttcaggccgc ccgtctggcg ggcgcgccgc agcagcagcc cggtcgcctt ctcgcacgtc
    12781 cactcgacgt cggcgaggcc cgcgctgcgc ttggcgtaga cgatcgtctg gcgcttggcc
    12841 ttctccgaca tcagatcgca gacgttggtc aggctctcgt tgtcgtcggc tgtctcgagc
    12901 gtttggatcg ccgggacgtc gacctccgcc acgccggcct cggcggccgc gtcgaagccc
    12961 tcgtagacct cgtctacggc ctcgacgccc tcgcgcgcct cctcgcggtc gccggtgagg
    13021 gctgggccgg agtcccgctg gccgccgtcg cgcttgtccg ccttggagcc cccgccctcg
    13081 cgcttcccgc cgctcgcctt cggcttggtc gtctccgtct gctccgcggc gcggggggag
    13141 ctctgcggct cgtcgtcgtc accgccgcag gcagcgaggc ccgcgcccgc gagcgagagg
    13201 gcgccgacga cggcgagcag tcggatgaag cggcgcatgt gagaggtgcc cagttccatg
    13261 gatacgaacg gacctagaca ctaaccggcg cgctggcgcc gtgcgcaggc tcggcggggc
    13321 ggcggccacg cggccgacgc gcggcggcgt gaaggcagcc acgtcagcgg gccatccgcc
    13381 actccccttc gcgcctcacg aacgtcagct tcgtagaggc gccgccggcg ccgggctgga
    13441 cctccgcctc ggcggagtcg ccgtcgatcc tcaatcgggc tatccgcggg cgggtacggt
    13501 cgaaggcgcc gtcgccatag acggctcgca ggccgttcga gcacctgcga ccctcatcga
    13561 ggctgcgccg gagcgtacgg gtcatggtcg cgcaagccgc ctcgaagtcg ccttcgtaga
    13621 cggcgtcggt gaaggccgcg taggtcgcgc ggatctgctt cgcggcgggg cccgtgccgt
    13681 cgacaggccc cttcgggtcg gtcgcctcat cgccggctcc gcatgccgag ccggcaaacg
    13741 cgcccagcgc tacggcgagg gtcgccgcga cggcgcccgc gagccggcgg cgtgattcgt
    13801 ggtcggtcat gggctcgaga gccagcgaca cgcggggtgg ggcgtgcgcc ccaccccgct
    13861 gtgcggctgc ttcagctcag ctgagctccg cccagccgca ggcgggctgc tccacgttct
    13921 gccccagcaa cagcttgtgg gcgttcaagc cgctagtcgc ccacatggcg tcgaaggcgg
    13981 cgtgcttgaa gttgttgaag ctaggaccgg ctgacccagc agcgtaggtg tgggtggcga
    14041 acgtcggcac cgacgggttg ccgatcatga tcccccgtgc gaagaacgtc ggccggtcgg
    14101 tcgcgagacc ggacggctgg acgagcacct gcgcgtagag cggcggaccg tcgttggtca
    14161 ggtgctgccg cacccacagc tcctgcgtcg gcttgtgcat gcagatctcg ccggtcgcgg
    14221 gcagcttcgc cggatcgacc gtgccgaggc cgcagtcggt cggcgtcacc ccggtgatcg
    14281 tgacgtcccc atccgagctg acatcgccgc tcagcgtcgc cgagcacgat ccgatcgtgt
    14341 gaatgccgaa gccggtaaag cttgtggtca gcaccgcgtt gtcgctgaac tcgcagactg
    14401 cgtcggggtg gccgcagagg tagcgcttcg agggggtgct gctccagtcg accgcattga
    14461 ccgccttcca gtcggccgcc atggccggat ggggcggctc cggcggacgc aacgtctgtg
    14521 ccatcgccga tcccgctgcg acggccagcg tgaggaccgc catcgtcgtc atcagtgtca
    14581 acctgcgcat attcatctcc tattgcttgg ttctcccgac cgcggccggt ggccacggct
    14641 gacttcacgg caggccgaac gcctgccgtc aaggtcgaac ccccctggcg cgctgtcagc
    14701 ggcggcgctt gaccgaccgc ttgcgcctgt ccgcgcggcg cttcttcttg ctcgccttgc
    14761 acggcgtcga gacccgcgtc ctgccatcgc gccggcggcc gttgtggccg tccatgtcga
    14821 ccgcggccac ctgacggcgc gagcacaggt cgatccgcga tctgcgcgtg cgcgtgaccg
    14881 ccaggatgcc gctgcgaccg cccgcgatcc tgaggtcgaa cctcgagatc ggcgcgtcgg
    14941 gcacgctggc gaacgtgttg accagccggt tgcgacgatc gacgctcgac tcgccgcgca
    15001 ggttgatcgc gacctcgccg cgcagcgcga cgacgatcat cggcaacgtg cggatcaggt
    15061 tcccggacga gcttcgccgc acgttcttga cgaagtagac attgccgctc agcgggccct
    15121 tgagcagcgg cgagaccgcc ctcgcgcgtc cgacgatcga ccccttcggg cagtgacgct
    15181 cgagatccgg cctgacgccg tcctcgtact cgcagagcgc gcgggcgttc tcgggatcca
    15241 gcgcaagggt cttcggcagc cggacccggg ccttctcgat ccccgcctcg ccggagcgct
    15301 gagtcaccct ggcgcgcacg ccgggatgcc tgccggtgcg catctgcttg cggccggtca
    15361 gccgcagcgc cagcttcggc ctgaacggca gcgccgcaca gcccgtcgcc tgatacgggc
    15421 tcgccggcct cgcgaccgcg ccctcgacgg aggtcacgtc ggccgcgatc cgcttcggcg
    15481 agcagtcggt cgggttgcgc atgaaccccg gccgatcgac cgtgacgatg atctgacgga
    15541 cccgcaacgg gatcccgtga tggatctgcg gcagcgggtc ggagaccgcc gtcaccgccg
    15601 ccgtgctcgg gtcgacgtga agcgcctggc gcacgacgat cggcgacagc gccagcgggc
    15661 cgcggaacgg gcccgcctcc acaggcaccg acaccgccag cccatacggc gcgcccttgt
    15721 agccctcggt caggaacacg tcacccttgc gctccaggaa gaacggcgcg cccgaccccg
    15781 caccggcatc cgccatgccg atccgcgacg ccgccggaca cgcgttcgcc gccgcctgcg
    15841 cgttcgagca cagcgccacg tcgcgcaccg ccgccagcag cccgcccggc atgctcaccg
    15901 acacgccggc cagccactgc tgaccctcct cgcgcgtcag gacgaatgag aacgccccgg
    15961 agccaccgcc ctgccggccg ttcatgccag cccgcaaccc gggcgcgaac ccgaagccgc
    16021 aacgctgatc ggtcacgaac gcgccgccgt cggagaccgg cagcccaccc gcgtcgtggg
    16081 cggccgtcca cggcgagaac tccgatgtcc agccgctcgc ctgacagcgc ggcggcaacg
    16141 ccagcagccc ccgatcgccg cccttgagcc gcatccgcat cgtctcgaac ggcacgcgcg
    16201 ggttcttgtc gaacgttgct gtcaaacgac ccgtctgcgg atcggcgacc gccgagccgt
    16261 agatcttcgc gatcaacccg cgctcgaccg accgcacgac cacgaacatc cgaaacatct
    16321 cgccggacgt cggatcggtc gacttcggca cgccgagcac gacctcgccg gtcaacggct
    16381 cgtcgagcag gggcgtgtcg acctcgaccg tcccgatctt ggacccggcg gggcactcgg
    16441 cgccgtccgc tcctccgtcc ttgtcgaacg ggtcgccgtt gttgaaccgc atcgggttgg
    16501 taccggagtc ggtcacgccg atgcccgcgt cgctgcaacc ctccaagccc gccgcgcccg
    16561 cagggttgac cgacacgccc gcgggcagcg acaccaccgc gcggtcgagc tgagaggtcg
    16621 caagcccggc gtcagacgcc caatggccgg cagctgcatc gacgtaggcg tcgacctccg
    16681 cttcgcccgc gccggcggcc ggcgcggcga acggcaggtc gtcgttctgc ggaaggtcga
    16741 ggcccgcgat cagccccgag gcggagtccg cgaccgtcga cgtcggctgg acggcgaggt
    16801 cggccgcgaa cgggaccttg tcgcatccgg tcacccgcgg cgcggggctc gcatagcgct
    16861 tccagttgac gtcgagcggg tcgggatcgc cctcgggcgt gaacgagccg ggagcctcgt
    16921 aggagtcgat cgccagccgg gtctcgagcg tcgcgcccga gcactcggtc gggttggaga
    16981 ggaacggctt gatctctccc cacgcgggcc gataggcgac ccggtcgtcc gggtcgagcc
    17041 cctgcggcgg gatctcgcca aagcggttgg gcgagctcga cgccggtcgc cacgccgtcg
    17101 gcgggcgcca gacgtcgtgg gacgagagcc acgggacgcc ccacagggtc acggactgcc
    17161 cgatgaccgg cagcgccgca ggcagctggg agacgaaggt ggtcacgccg aagtcgccgt
    17221 tggtgcgcgc cttcgcgacg atccgcgccg tggtggcgtc ctcgccgctg acatacgcga
    17281 cgccgagctc ggcgacgttg cccttgcgcg gctcgaggtt gtagaccggc aggatctcct
    17341 cggtggaggc ctggtagttg acgccgccgc cggcgccggc gccgacgatg tagaggtgca
    17401 gcaccccgac ctgggtctcg ggcgggcact gcagcggcct gacggcgaac tgctcggcgg
    17461 tgcacttggg gaccgcggtc ggatcgccga cgaatccggc cggcagctcg ccgtagacgt
    17521 tgtcgagcgt gccgtcgacc agccgatccg agttgcgtgc gagccagaac gtcgccgagc
    17581 cgtcggggtg ggcgcctgcc tgggtcaccg gggcgaggcg ccagctcggc ggtgcgcgcc
    17641 agatcggccg atccggcggc ggcgagccgt gcggcgccag gcgcccccag tcgctgcact
    17701 ggtcgggcgt ggctggcgcc ggcaccgcga cgttctgccc ggtgggcggg ctgcccgacg
    17761 gaaccggctc gggcgcccag accgtcgcgg
//
LOCUS       DATABV010000127         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2770593, whole genome shotgun sequence.
ACCESSION   DATABV010000127 DATABV010000000
VERSION     DATABV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2770593"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="pgk"
                     /locus_tag="VHF88_05520"
     CDS             <1..332
                     /gene="pgk"
                     /locus_tag="VHF88_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX2161264.1"
                     /translation="VPEGWMGLDIGAATAEAYAREVKAAGTVFWNGPMGAFEMEPFAA
                     GTRAVAEAVAEAAGTTVVGGGDSAAALASFGLAERVTHMSTGGGAALELLEGKALPGV
                     EALDDAQ"
     gene            322..1095
                     /gene="tpiA"
                     /locus_tag="VHF88_05525"
     CDS             322..1095
                     /gene="tpiA"
                     /locus_tag="VHF88_05525"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX2161265.1"
                     /translation="MPSDIDAGRRPYIAGNWKLWGTRAQAADYCERLLPLISGTARVD
                     VGLCVPYTALETVAGALDGSGVVVAAQNMHQAETGAFTGEVSAAMLLELGADAVVLGH
                     SERRELFGETDRALSEKLPAALDAGLQPILCVGETEQEREAGDMERKLRHQVQEGLEN
                     VADDRLADVVIAYEPIWAIGTGKVATPDQAQEAISFVRALVGDRSAAAAERVRLLYGG
                     SVKPENAAEILEQADVDGALVGGASLDPDSFARIVAAAG"
     gene            1092..2693
                     /gene="gpmI"
                     /locus_tag="VHF88_05530"
     CDS             1092..2693
                     /gene="gpmI"
                     /locus_tag="VHF88_05530"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX2161266.1"
                     /translation="MTVPAVCLVILDGWGLAPPGPGNAVSLADTPVFDGLWERHRHTQ
                     LTACGRAVGLPDGQMGNSEVGHLNLGAGKIVRQDLARIDDAVAGGEFAENPALRDACA
                     AARESGRLHLLGLVSDGGVHASMDHLRALIELAASFGVPDLILHAFTDGRDTLPDSGA
                     GYLATVEGWLAEHGGRVATVSGRYYAMDRDRRWPRVKLAYDAIVHGRPAGLGNDPDDF
                     PGAEGGSSVSTEAAATTPHTASAVAAVSAAYARGETDEFVRPTLVGEEGRVRDGDAVI
                     CFNFRPDRARELTRALVEPGFAEFDRGAAPRVHYTTLTEYQEDWRYPVAFPPERPDAT
                     LASVLADRGVRQLHVAETEKYPHVTYFFNGGEEHPYAGEEHCLVDSPRDVPTYDHKPE
                     MSAEAAASALVERWNAGGYGFGIINFANPDMVGHTGSIPAAVAAIEAVDVQLGRIVEA
                     VHGSGGACIVTADHGNADHMLEPDGSPNTAHSLNPVPLIVTADVPGLRDGGILADVAP
                     TVLELLGVAQPAEMTGKSLLVGPQT"
BASE COUNT          396 a          995 c         1000 g          384 t
ORIGIN      
        1 aggtgccgga gggatggatg gggctcgaca tcggcgcggc gacggcggag gcttacgcgc
       61 gggaggtgaa ggcggcgggg accgtgttct ggaacggccc gatgggcgcg ttcgagatgg
      121 agccgttcgc ggccgggacc cgagcggtcg ccgaggcggt cgccgaggct gcgggcacca
      181 cggtcgtcgg cgggggcgat tcggccgccg cgctcgcctc gttcggtctg gcagagcgcg
      241 tcacgcacat gtcaacgggc ggcggcgccg ccctcgagct gctggagggc aaggcccttc
      301 cgggagtgga ggccctagac gatgcccagt gacattgacg ccggccgccg gccgtacatc
      361 gccggcaact ggaagctctg ggggacgcgc gcccaggcgg ccgactactg cgagcggctg
      421 ctgccgctga tctccggcac ggctcgggtc gacgtcggcc tctgcgtgcc gtatacggcg
      481 ctcgagacgg tcgccggcgc gctcgacggg tcgggcgtcg tggtcgccgc ccagaacatg
      541 caccaggccg agaccggcgc gttcacaggc gaggtctccg cggcgatgct gctcgagctc
      601 ggcgcggacg ccgtggtgct cggccactcc gagcggcgcg agctgttcgg cgagaccgac
      661 cgcgcgctgt cggagaagct gcccgcggcc ctcgacgcgg gtctgcagcc gatcctctgc
      721 gtcggcgaga ccgagcagga gcgcgaggcc ggggacatgg agcgcaagct gcgccatcag
      781 gtccaggagg ggctggagaa cgtcgccgac gaccggctcg ccgacgtcgt gatcgcatat
      841 gagccgatct gggcgatcgg gaccggcaag gtcgccacgc ccgaccaggc gcaggaggca
      901 atctccttcg tcagggcgct ggtaggggac cgctcggccg ctgccgccga gcgcgtgcgc
      961 ctgctctacg gcggcagcgt caagccggag aacgccgcgg agatcctcga gcaggccgac
     1021 gtcgacggag cgctggtcgg gggggcgagc ctcgatcccg acagcttcgc gcggatagtc
     1081 gcagcagcag ggtgaccgtc cctgctgtct gcctggtgat cctcgacggc tgggggctcg
     1141 cgccgcccgg ccccgggaac gccgtctcgc tcgccgacac gccggtcttc gacgggctct
     1201 gggagcgcca ccgccacact cagctgaccg cctgcggtcg cgccgtcggc ctgcccgacg
     1261 gtcagatggg caactccgag gtcggccacc tcaacctagg cgcgggcaag atcgttcgcc
     1321 aggatctggc gcggatcgac gacgcggttg ccggcggcga gttcgccgag aacccggcgc
     1381 tgcgcgacgc ctgcgccgcc gcgcgcgaat ccggtcgcct gcatctgctc ggcctcgtct
     1441 ccgacggagg cgtgcacgcc agcatggacc acctacgggc gctgatcgag ctcgccgcca
     1501 gtttcggcgt tcccgacctg atcctgcacg ccttcaccga cggtcgcgac acgctgcccg
     1561 actcgggcgc tggttacctc gccaccgtcg agggctggct cgccgagcac ggcggccgcg
     1621 tggcgaccgt cagcggtcgc tactacgcca tggaccgcga ccgccgctgg ccgcgcgtca
     1681 agctcgccta cgacgcgatc gtccacgggc gtcccgccgg ccttgggaac gacccggacg
     1741 acttccccgg cgccgagggg ggatcgagcg tcagcaccga ggccgctgcc accacgccgc
     1801 acaccgcgtc cgctgtggcg gccgtcagcg ccgcttacgc ccgcggcgag accgacgagt
     1861 tcgtccgccc gacgctggtc ggcgaggagg ggcgcgtccg cgacggcgac gcggtcatct
     1921 gcttcaactt ccgccccgac cgcgctcgcg agctgacccg cgcgctcgtc gagcccggct
     1981 tcgccgagtt cgaccggggc gccgcgccgc gcgtccacta cacgacgctg accgagtacc
     2041 aggaggactg gcgctacccg gtcgctttcc cgcccgagcg ccccgacgcg acgctggcgt
     2101 cggtgctcgc cgaccgcggc gttcgccaac tccacgtcgc cgagaccgag aagtacccgc
     2161 acgtaacgta cttcttcaac ggtggggagg agcaccccta tgccggcgag gagcactgcc
     2221 tggtcgactc gccgcgcgac gtgccgacct acgaccacaa gcccgagatg agcgccgagg
     2281 cggcggcgtc tgcgctcgtc gagcgctgga atgccggcgg ctatggcttc gggatcatca
     2341 acttcgcgaa ccccgacatg gtcggtcaca cgggctcgat ccctgcggcg gttgcggcga
     2401 ttgaggcggt cgacgtccag ctcggccgca ttgtcgaggc ggtccacggc tccggcggcg
     2461 cctgcatcgt caccgccgat cacggcaacg ccgaccacat gctcgagccg gacggcagcc
     2521 cgaacaccgc ccactcgctg aaccccgtgc cgctgatcgt caccgccgac gtgcccgggc
     2581 tgcgggacgg cgggattctc gccgacgtcg ccccgacggt gctcgagctg cttggcgtcg
     2641 cccagccagc tgagatgacc ggaaagagcc tgctcgtagg cccccagact tgacacgcgg
     2701 agactcaggt cttatactgg cgctcgcata agaagtaacg cgtagtctgt ttgtcgagga
     2761 ggtatttgcg atgag
//
LOCUS       DATABV010000128         4458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2129110, whole genome shotgun sequence.
ACCESSION   DATABV010000128 DATABV010000000
VERSION     DATABV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129110"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VHF88_05535"
     CDS             complement(<1..482)
                     /locus_tag="VHF88_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2161267.1"
                     /translation="MREIRLHDTLSGTLRPLEPREPGKVGIYACGPTVYARVHVGNAR
                     PYVVFALLKRFLEHEGYEVTLVENVTDVNDKIYAAAREAGISSQELAAEMTRAYEQDT
                     ARLGLGRPDHEPRAVDTIGPIVDLIQALIDGGHAYAAGGDVYFDVSSFADYGKLSNRP
                     R"
     gene            complement(505..1905)
                     /locus_tag="VHF88_05540"
     CDS             complement(505..1905)
                     /locus_tag="VHF88_05540"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF009119.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX2161268.1"
                     /translation="MIDDDLAFAGLAKQAALVRAGEVSSRELVELYLERIARIEPQLN
                     CYSVVLAERALIEAEQADSRRAAGDERPLLGVPVAIKDNVDVAGTVTTFGTAAYGPPA
                     TEDSELVRRLRSAGCVVLGKTTLPELAIMGTTEGPAFGVTRNPWDVDRTPGGSSGGSG
                     AAVAAGLAAAGHASDGAGSIRIPAACCGLFGLKPQRGRVPLAPDEENTWYGLTVAGAV
                     TRTVLDNALFLDAVAGPGPAPGGPTLDRPFADAARELPGKLRIAVSMRPPLLARLSPT
                     VRQAVDETADLLRGLGHDVEYVEPPLTDWPTELLPRYLKGIESDAARMARPERLQRRT
                     RSFARLGSLIPQAMLDRALRIEQQVSERYNRVLADRDVLLTPTTAMSAPDAARWEGVG
                     ALRTLVEMIMAYPYCVIWNATGQPAAAVPAGFTADGLPLSAQLIAKPNQDHLLLSLAA
                     QIEAERPWADKRPPLS"
     gene            complement(1963..2457)
                     /gene="ispF"
                     /locus_tag="VHF88_05545"
     CDS             complement(1963..2457)
                     /gene="ispF"
                     /locus_tag="VHF88_05545"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX2161269.1"
                     /translation="MNVRSGIGYDSHRLADGRALILGGVEIEDAELGLEGHSDADALT
                     HAVIDALLGAAGLGDIGLHFPDSDERWRDADSIALLREVVSLIAGAGWSVGNVDATLI
                     CEAPKLGPYRDRMRAALATAVGVNPDAVNVKFTTNERMGPIGRGEGIAALATATLSAR
                     ANDR"
     gene            complement(2454..3278)
                     /locus_tag="VHF88_05550"
     CDS             complement(2454..3278)
                     /locus_tag="VHF88_05550"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX2161270.1"
                     /translation="MLTDYHVHLRPDEDDTPAAEYFTAANVERYRAAAAERGIGELGV
                     SEHVYRFAQALDVWQHPFWRANAVDDLDAYCEFLRAHGLRVGIEADFVVGHEDRMASL
                     LEARDWDYVVGAVHFLRDAAVDMRGDWDVWRSGDPDKVWARYFETLGEAARTGMFDIL
                     AHPDLVKVWGKGAPTPDGDLRRFYELALEGIAESDVAIEVSTAGLRKPVGELYPAAPF
                     LELCLEVGRPVALSSDAHVPEQLGYEYERAVEWLAGIGVTELAVFEGRERRLEPIG"
     gene            complement(3290..3949)
                     /gene="ispD"
                     /locus_tag="VHF88_05555"
     CDS             complement(3290..3949)
                     /gene="ispD"
                     /locus_tag="VHF88_05555"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX2161271.1"
                     /translation="MRVAIVAAGGSGERLGADGPKAFVDCAGRPLVDWSIEVLGAVCD
                     RVVVAVPRGFEKGEDGPERVAGGASRSESVRNALAAAPEAEVAVVHDAARPLVSTALV
                     ERCVAAIHAPGVTGAVAAAPVTDTIKEADEAGRVLRTLDRGTLWSVQTPQVFWAEPLR
                     AALEADPDTLAAATDDAGLVEAAGGTVAIVEAPRENIKVTTALDLSVAELLLGRRTAD
                     A"
     gene            complement(3940..>4458)
                     /locus_tag="VHF88_05560"
     CDS             complement(3940..>4458)
                     /locus_tag="VHF88_05560"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX2161272.1"
                     /translation="IEYEIGDNVVYPHHGAGKVLKKEQKTVLGEQREYLTIKILHNDM
                     TVMVPCENAGKAGLRRVIGEEDVARVLAVLRDDVSEMQKNWNRRFKHNREKIKTGDVY
                     ELAEVVRNLAIRESEKGLSTGEKQMYTRAKKILASELMYALEMEEDEAEQHLDSLIED
                     AHSDRMGAAASA"
BASE COUNT          598 a         1646 c         1570 g          644 t
ORIGIN      
        1 cgcggacggt tcgagagctt gccgtagtcg gcgaagctcg agacgtcgaa gtagacgtcg
       61 ccgcccgcgg cataggcgtg gccgccgtcg atcagcgcct ggatcaggtc gacgatcggg
      121 ccgatcgtgt cgaccgcgcg cggctcgtgg tcgggccggc cgaggccgag ccgcgcggtg
      181 tcctgctcgt aggcgcgcgt catctcggcc gccagctcct ggctggagat gccggcctcg
      241 cgcgcggcgg catagatctt gtcgttgacg tcggtgacgt tctcgaccag cgtcacctcg
      301 tagccctcgt gctcgaggaa ccgcttcagc agcgcgaaca cgacgtaggg acgcgcgttg
      361 ccgacgtgca cgcgcgcata gacggtcggc ccacaggcgt agatgccgac cttgccgggc
      421 tcgcgcggct cgagtgggcg cagcgtcccg ctcagcgtgt cgtgcaggcg gatctccctc
      481 acaggcggga gggtatagcc gccgtcagga cagcgggggg cgcttgtcgg cccagggacg
      541 ctcggcctcg atttgggccg ccagcgaaag cagcaggtgg tcctggttcg gcttggcgat
      601 cagctgcgcc gagagcggca ggccgtcggc cgtgaagccg gcgggcaccg cggcggccgg
      661 ctgaccggtc gcgttccaga tcacgcagta cggataggcc atgatcatct cgacgagcgt
      721 ccgcagcgcg ccgaccccct cccaacgggc cgcgtcaggc gccgacatgg cggtggtcgg
      781 cgtcagcagg acgtcgcggt ccgcgagcac gcggttgtag cgctcggaga cctgctgctc
      841 gatgcgcagc gcgcggtcga gcatcgcctg cgggatcagc gatccgagcc gcgcgaagga
      901 gcgcgtgcgg cgttgcagcc gctccgggcg cgccatgcgc gcggcgtcgc tctcgatgcc
      961 cttgaggtac cgcggcagca gctcggtcgg ccagtccgtc agcggcggct cgacgtactc
     1021 gacgtcgtgt ccgagaccgc gcagcaggtc ggccgtctcg tcgacggcct gccgcaccgt
     1081 cggcgacagc cgggcgagca acggcggccg catcgacacg gcgatccgca gcttgcccgg
     1141 aagctccctg gcggcgtcgg cgaacgggcg atcgagcgtc gggcccccgg gagcggggcc
     1201 gggaccggcg accgcgtcga ggaacagcgc gttgtcgagc accgtccgcg tgaccgcccc
     1261 cgcgacggtc aggccgtacc acgtgttctc ctcgtcgggg gcgagcggca cacggccgcg
     1321 ctgcggcttg agcccgaaca gcccgcagca ggcggccggg atgcggatcg agccggcgcc
     1381 gtcgctggcg tgtccagccg cggcgagccc cgccgccacc gcggcgccgc tgccgccgct
     1441 cgagccgccg ggggtgcgat cgacgtccca cgggttgcgc gtgaccccga aggccgggcc
     1501 ctcggtcgtg cccatgatcg ccagctcggg cagcgtggtc ttgccgagca ccacgcagcc
     1561 ggcggagcgc agccgccgga ccagctccga gtcctccgtc gcgggtgggc cgtaggccgc
     1621 tgtgccgaag gtcgtcacgg tcccggccac gtcgacgttg tccttgatcg ccaccggaac
     1681 gccgagcagc ggccgctcgt ctccggcggc gcggcgcgag tcggcctgct cggcctcgat
     1741 cagcgcccgc tcggccagca cgacgctgta gcagttcagc tggggctcga tccgcgcgat
     1801 ccgctccaga tacagctcca ccagctcgcg cgaggacacc tcgcccgcgc ggaccagcgc
     1861 ggcttgcttg gccaggcccg cgaacgccag atcgtcgtcg atcatcgcgg ggactctacc
     1921 gctgcctcag tcaccgagcg aaggcctatc ggtgctggag gcctatcgat cgttcgcgcg
     1981 cgcggagagc gtcgccgtcg ccagcgcagc gatgccctcg ccgcggccga tcgggcccat
     2041 gcgctcgttg gtggtgaact tgacgttcac ggcgtcgggg ttgacgccga ccgccgtcgc
     2101 cagcgcagcc cgcatgcggt cgcgatacgg cccgagcttg ggcgcctcgc agatcagcgt
     2161 cgcgtcgacg ttgccgacgc tccagccggc gcctgcgatc agcgagacga cctcgcgaag
     2221 cagcgcgatc gagtcggcgt cgcgccagcg ctcgtcgctg tctgggaagt gcaggccgat
     2281 gtcgccgagg cccgcggcgc cgagcagcgc gtcgatcacc gcatgcgtca gcgcatcggc
     2341 gtcggagtgc ccctcgaggc cgagctccgc gtcctcgatc tcgacgccgc ccaggatcaa
     2401 cgcgcgccca tccgccaagc ggtgcgagtc gtagccgatc ccgctgcgca cgttcacccg
     2461 atcggctcca gccgccgctc gcggccctcg aagacggcga gctcggtgac gccgatgccc
     2521 gccagccatt cgaccgcccg ctcgtactcg tagccgagct gctctggcac gtgcgcgtcg
     2581 ctcgacagcg cgaccgggcg ccccacctcg aggcacagct cgaggaaggg agcagcggga
     2641 tacagctcgc cgaccggctt gcgcagcccg gcggtcgaga cctcgatcgc cacgtcggac
     2701 tccgcaatcc cctcgagcgc cagctcgtag aagcggcgca ggtcgccgtc gggcgtcggc
     2761 gctcccttgc cccacacctt cacgaggtcg ggatgggcga ggatgtcgaa catgcccgtc
     2821 cgggccgcct cgccgagcgt ctcgaagtag cgcgcccaaa ccttgtcggg atccccggag
     2881 cgccagacgt cccagtcgcc gcgcatgtcg accgcggcgt cgcgcaggaa gtggaccgca
     2941 ccgacgacgt agtcccagtc gcgcgcctcg agcaggctgg ccatccggtc ctcgtgaccg
     3001 acgacgaagt cggcctcgat gccgacgcgc agcccgtgcg cgcgcaggaa ctcgcagtag
     3061 gcgtcgaggt cgtcaaccgc gttcgctcgc cagaaggggt gctgccagac gtcgagcgcc
     3121 tgggcgaagc ggtagacgtg ctcggacacg ccgagctcgc cgatgccccg ctcggctgcc
     3181 gcggcccggt agcgctcgac gttggcggcc gtgaagtact cggcggccgg cgtgtcgtcc
     3241 tcgtcgggcc gcagatggac gtggtagtcg gtcagcacgg cccggcagac tacgcgtcgg
     3301 cggtccgccg tccgagcagc agctcggcga cgctgaggtc gagcgccgtc gtgaccttga
     3361 tgttctcgcg cggcgcctcg acgatcgcga ccgtcccgcc cgccgcctcg acgagcccgg
     3421 cgtcgtcggt cgccgcggcc agcgtgtccg gatctgcctc cagcgcggcg cgcagcggct
     3481 cggcccagaa gacctgcggc gtctgcaccg accagagcgt cccgcggtcg agcgtgcgca
     3541 ggacgcgacc agcctcgtcg gcctccttga tcgtgtcggt caccggcgcc gcggccaccg
     3601 cgccggtcac tcccggcgcg tggatcgcag cgacacaacg ctcgaccagg gcggtgctga
     3661 ccagtggccg cgccgcatcg tgcacgaccg cgacctcggc ctccggcgcg gcggcgagcg
     3721 cgttgcggac cgactccgac cgcgaggcgc caccggcgac gcgctcaggg ccgtcctcgc
     3781 ccttctcgaa gccgcggggc accgccacca ccacccggtc gcagacggcg cccaagacct
     3841 cgatgctcca gtcgaccagc ggccggccgg cgcagtcgac gaacgccttc gggccgtcgg
     3901 ccccgagacg ctcgccgctg ccgccggcgg cgacgatcgc tacgcggatg cggcggcccc
     3961 catgcggtcc gagtgagcgt cctcgatcag cgagtcgagg tgctgctcgg cctcgtcctc
     4021 ctccatctcg agcgcgtaca tcagctcgga ggcgaggatc ttcttggcgc gcgtgtacat
     4081 ctgcttctcg ccggtcgaga gccccttctc ggactcccgg atcgcgaggt tgcggacgac
     4141 ctcggccagc tcgtagacgt cgccggtctt gatcttctcg cggttgtgct tgaagcggcg
     4201 gttccagttc ttctgcatct cgctgacgtc gtcgcgcagc accgcgagga cccgggcgac
     4261 gtcctcctcg ccgatcacgc ggcgcagccc ggccttgccc gcgttctcgc acgggaccat
     4321 caccgtcatg tcgttgtgaa ggatcttgat cgtcaggtac tcgcgctgct cgccgagaac
     4381 cgtcttctgc tccttcttga ggaccttgcc ggcgccatgg tgcgggtaca ccacgttgtc
     4441 cccgatctcg tactcgat
//
LOCUS       DATABV010000129         8210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_442472, whole genome shotgun sequence.
ACCESSION   DATABV010000129 DATABV010000000
VERSION     DATABV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442472"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VHF88_05565"
     CDS             <1..218
                     /locus_tag="VHF88_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX2161273.1"
                     /translation="ESPLQGNMWKVVVKQGDVVEEGQLLCIIEAMKMENEITAHKAGT
                     IAALPIEEGKPISAGDPIATITSAPSE"
     gene            226..1965
                     /locus_tag="VHF88_05570"
     CDS             226..1965
                     /locus_tag="VHF88_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX2161274.1"
                     /translation="MAVAPQPSRRDRLSYVRDRLAGILRPRVSVSEPPAGAVASELDV
                     AVPMRDGVVLRVNVYRPPGDEPVPAIMSAHPYGKDVLPRRGRRGRWRFSPQYRAFRQP
                     QPVAFSSLTGWEAPDPAWWVARGYAVVNCDLRGCGTSDGTGDLLTAKEGEDYHDLIEW
                     AAVQPWCTGKVGLLGVSYLAISQYEAAATRPPHLAAICPWEGFSDPYRDLMRPGGVRE
                     DGFVRVWSRGVRKWRLTSDVRAGQVAHELCDEWWQARTPTVERIEAPMLVCASFSDHN
                     LHSHGSFRAFEQAGSRQKWLYTHRGGKWATFYSADALAAQRRFFDHFLKGEDNGQAEQ
                     SPVRLEVRSDRDTVVEVRQEQEWPLRATDWTELRLSADGRLIVGGAEPAGASEPGAHE
                     RDGHLAEGAVRFETDSGVAIFRRAFAERTELSGPMALRLHVSIEGGDDADLHVRVHKV
                     RDGRAVPFEGSYGYGLDGVTTGWLRISQRELDVERSRPFEPVHTHARRLPLAPGEIAP
                     VEIALMPSATLFEAGDELVLEVHGRWPKPRNPITGQFPAWYERRPAVRCALHCGGERD
                     ARLLVPVIRRASG"
     gene            1992..3215
                     /locus_tag="VHF88_05575"
     CDS             1992..3215
                     /locus_tag="VHF88_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2161275.1"
                     /translation="MPGGAPTASRARRLLPLVLATTAMQASIVVLAPLLVEIGQTLGA
                     SVSAVGVARSVLAGTAFAGSLAIGPLIDRIGVRPLVVRGAALALAGVVASAASPTLLV
                     FYAAHVVTGLGVACLLSAGFAGVATYFSGREMAWAMGYVVGAQSLAWIVGNPIVGALA
                     DAGSWRLAYAVPASVCLAALLAGFLLRPLERTDAVPEPIRDGLRAVFRDRSARRWTIA
                     ELVAYSAWSAELTYAAVFYIRNYETSVTTVGFLLAAGSLVFLVTSLNTARITMRFARK
                     PLLVASALGMGLVLVPILNWAPSVAGTFAMFCAMATFAAIRLAGSSALGLDQLPDRPG
                     AMMGARTAAAQLGYMVGAAGGGGVLALWGFGTLGFVLCAGMALSAVLLTGVHDPWSER
                     QRAGAAEALPAPLVD"
     gene            complement(3367..4002)
                     /locus_tag="VHF88_05580"
     CDS             complement(3367..4002)
                     /locus_tag="VHF88_05580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2161276.1"
                     /translation="MAESVIFDCDGVLVDTEPAANVVLADLITELGIPTTPEQALAEY
                     MGRSWASCLEIVGRKLGHPPPDDFGDRYRAGVTAAWKRDLRPVPGVVEALDAIDLPSC
                     VASSGEHERMRLTLGLTDLLPRFEGRLFSATEVQHGKPAPDLFLHAADRMGFQPSETA
                     VVEDTVPGVQAGRAAGMRVLAFARLVAADDLAAAGGEVFDDMRVLPALLAV"
     gene            4074..4526
                     /locus_tag="VHF88_05585"
     CDS             4074..4526
                     /locus_tag="VHF88_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX2161277.1"
                     /translation="MAIDFDAVRAGLEQAIPFNKHLALEVVAVADGSGSVRLPDDPAL
                     LNHVGSQHAGALFSVAEAASGAAFVGAFAERMGEITPLAKSASIQYRKLAKGPIVATG
                     TLSKDKASLLERLDADGRVEFTIDVSLRDADDVEVATVTVDWHVRKNG"
     gene            complement(4672..5448)
                     /locus_tag="VHF88_05590"
     CDS             complement(4672..5448)
                     /locus_tag="VHF88_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2161278.1"
                     /translation="MRAVSVHSDAIVVTSLLWQTNAIALRAGGEAMLIDSPYFPDELE
                     LLPGVLAQSGFEPNALLATHGDFDHLLGRLAFPGLALGVSEGTMRRIRERPGEAQREL
                     RDADAEHYVERGRPLSLGEVQSLPVPGQLELGDQELQLATAEGHTPDGMAVWAPWLGV
                     LCTGDYVSPVEIPWISAGGSIGAYRDTLARLAPLVEQAEAVVPGHGVPQSRAAALRLI
                     DEDLEYLDTLERGDERPKLPDGRDSARQRHIHAKNLAQLR"
     gene            complement(5460..6143)
                     /locus_tag="VHF88_05595"
     CDS             complement(5460..6143)
                     /locus_tag="VHF88_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2161279.1"
                     /translation="MPLPKDIQFVTFDCYGTLIDWETGVYDAFQKEADRDGFTIDRDV
                     LVPRFIEIQRKIQSGSYELYAEVLRRTAVQVAEELGWGLESSRAQFLPDSVPRWQPFR
                     ETNAQLERFAKKYELGILSNIDDKLLGATRRHFRSDFDLVVTAQQVRSYKPDPAHFKE
                     CARRIEKRKKNWVHIASGYPTDVEPCLKSKVPVIWVNRHGYELESGQKKPTEEVKTFR
                     DAAKLLGLV"
     gene            6249..7403
                     /gene="sucC"
                     /locus_tag="VHF88_05600"
     CDS             6249..7403
                     /gene="sucC"
                     /locus_tag="VHF88_05600"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX2161280.1"
                     /translation="MDLLEYQGKQLFARHGIPVPQGKPAQTVDEAVAAAEELGYPVVV
                     KAQVLVGGRGKAGGVKLAADRGEVEQHAGAILGMDIKGHTVHELYIEAASDIDEEYYA
                     AFVFDRSAKKPMAMFSKMGGMDVEAVAETDPEAMRTLHVDPLLGFQDFQGRRLAFEAG
                     IPADVIRPVGALLAKLYEVFVAEDAMLVEVNPLLITKSREAVALDAKVTLDGNALFRH
                     PYAAEFGDKGAADPQEKMAKERGLTYVKLDGNIGILGNGAGLVMSTLDVVAQAGGQPA
                     NFLDAGGGAKAEEITSAVEVILSNENVSAVLFNIFGGITRCDEVANGLVEAFKQVNPD
                     VPFVVRLDGTNDEEGRRILQEANLSGLYVEKTMLEAAERVVALAKEGGAK"
     gene            7400..>8210
                     /gene="sucD"
                     /locus_tag="VHF88_05605"
     CDS             7400..>8210
                     /gene="sucD"
                     /locus_tag="VHF88_05605"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX2161281.1"
                     /translation="MSILIDKDTKLCVSGITGREGTFHTLNNKRYGTNVVAGVTPGKA
                     GQDVEGIPVFNTFAKAVEETGANTAMVFVPPRFAADSILEAEDAGIELIVAITEGIPA
                     HDELRVYNHLARNPDVRLVGPNCPGILSPGKANVGIIPAAFFSEGNVGVVSRSGTLTY
                     QIGNELAQRGFGNSTIVGIGGDPVPGSSFIDVLARFQEDDETELIVMAGEIGGSAEEE
                     PAEFIAENVTKPVVAYIAGFTAPPGKTMGHAGAIVSGSSGTAAAKAEALESK"
BASE COUNT         1148 a         2915 c         2951 g         1196 t
ORIGIN      
        1 tcgagtcgcc gctgcagggc aacatgtgga aggtcgtcgt caagcagggc gacgtggtcg
       61 aggagggcca gctgctctgc atcatcgagg cgatgaagat ggagaacgag atcaccgccc
      121 acaaggcggg cacgatcgcc gcgctgccga tcgaggaggg caagccgatc tcggctggcg
      181 acccgatcgc gaccataacc tccgccccgt ccgagtagcc gctcggtggc ggtcgcgccg
      241 cagccttcgc gccgcgaccg cctgagctac gtacgcgacc ggcttgcggg aatcctgcgc
      301 ccgcgcgtga gcgtcagcga gccgcccgcg ggggccgtgg cgagcgagct cgacgttgct
      361 gtgccgatgc gcgatggcgt cgtgctgcgc gtcaatgtct accggccgcc gggcgacgag
      421 ccagtcccgg cgatcatgtc ggcccatccc tacggcaagg acgtcctgcc gcgccgcggg
      481 cggcggggcc gctggcgctt ctcgccgcag taccgcgcgt tccgccagcc ccagccggtc
      541 gcgttctcgt cgctgaccgg ctgggaggcg cccgacccgg cgtggtgggt ggcgcgcggc
      601 tacgccgtcg tcaactgcga cctgcgcggc tgcgggacgt cagacggcac cggtgacctg
      661 ctgactgcca aggagggcga ggactaccac gacctgatcg agtgggccgc cgtccagccc
      721 tggtgcacgg gcaaggtcgg gctgcttggg gtctcctacc tggcgatcag ccagtacgag
      781 gctgctgcga cgcggccacc gcacctcgcg gcgatctgcc cgtgggaggg cttcagcgat
      841 ccgtaccgcg acctgatgcg ccccggcggc gtgcgcgagg acggcttcgt ccgcgtctgg
      901 agccgcggcg tgcgcaagtg gcgactcacg tcggatgttc gagcgggcca ggtcgcccac
      961 gagctgtgcg acgagtggtg gcaggcgcgc acgccgacgg tcgagcggat cgaggcgccg
     1021 atgctcgtct gcgccagctt ctcggaccac aacctgcaca gccacggctc tttccgggcg
     1081 ttcgagcagg ccggctcgcg acagaagtgg ctctacaccc accgcggcgg caagtgggcg
     1141 acgttctact cggcggacgc gctcgccgcc cagcggcgct tcttcgacca cttcctgaag
     1201 ggcgaggaca acggccaggc cgagcagtcg ccggtgcggc tcgaggtgcg cagcgatcgc
     1261 gacacggttg tcgaggttcg tcaggagcag gagtggccgc tgcgcgcgac ggactggacg
     1321 gagctgcggc tgagcgcgga tgggcggctg atcgtgggcg gcgccgagcc ggccggcgcc
     1381 agcgagcccg gcgcgcacga gcgcgacggt catctcgccg agggcgccgt tcggttcgag
     1441 acggactccg gcgtcgcgat cttccgccgg gcgttcgccg agcgcaccga gctctcgggc
     1501 ccgatggcgc tgcgcctgca cgtctccatc gagggcggcg acgacgccga cctccacgtt
     1561 cgcgtccaca aggtccgtga cgggcgggcc gtccccttcg agggctcgta cgggtatggg
     1621 ctcgacggcg tcaccaccgg ctggttgcgg atctcccagc gcgagctcga cgtcgagcgc
     1681 tcgcgcccgt tcgagccggt ccacacgcat gcgcgccgac tcccgctcgc ccccggcgag
     1741 atcgctccgg tcgagatcgc gctgatgccg tcggcgacgc tgttcgaggc cggcgacgag
     1801 ctcgtgctcg aggttcacgg tcgctggccg aagccgcgca acccgatcac cggccagttc
     1861 ccagcgtggt acgagcggcg gcctgcggtc cgatgcgcgc tgcactgcgg cggcgagcgc
     1921 gacgcgcggc tgctggtgcc ggtgatccgg cgcgcgagcg gctgacaagc ggcctgcgat
     1981 ccttggcgcc cgtgcccgga ggcgccccga cagccagccg tgcccggcgg ctgctgccgc
     2041 tggtgctagc gacgaccgcg atgcaggcgt cgatcgtcgt gctggcgccg ctgctggtcg
     2101 agatcggcca gacgctcggc gcctcggtga gcgccgtcgg cgtggcgcgc tcggtgctcg
     2161 cggggacggc cttcgcgggc tcgctcgcga tcggtccgct gatcgaccgg atcggcgtgc
     2221 ggccgctggt cgtgcgcggc gccgcgctcg ctctggcggg cgtcgtagcg agcgcggcgt
     2281 cgccgacgct gctcgtcttc tacgccgctc acgtggtgac ggggctcggt gtggcctgcc
     2341 tgctgtcggc cggcttcgcc ggcgtcgcga cttacttcag cggccgcgag atggcgtggg
     2401 cgatgggcta cgtggtcggc gcgcagtcgc tcgcctggat cgtcggcaac ccgatcgtcg
     2461 gggcgctcgc ggacgccggc tcctggcggc tcgcctacgc cgtgcctgcg agcgtatgcc
     2521 tggcggcgct gctggccggc ttcctgctgc gaccgctcga gcgcaccgac gcggtgccgg
     2581 agccgatccg cgacggcctg cgcgcggtct tccgcgaccg ctcggcgcgg cgctggacga
     2641 tcgccgagct cgtcgcctac tcggcctggt cggccgagct gacctatgcg gccgtcttct
     2701 acatccgcaa ctacgagacg agcgtcacga cggtcggctt cctgctcgcc gccggctcgc
     2761 tggtgttcct ggtgacctcg ctgaacacgg cgcgaatcac gatgcggttc gcgcgcaagc
     2821 cgctgctcgt cgcctctgcg ctcgggatgg gcctcgtgct cgtcccgatc ctcaactggg
     2881 cgccgtcggt cgccgggacg ttcgcgatgt tctgcgcgat ggcgacgttc gcggcgatcc
     2941 ggctcgccgg ctcgagcgcg ctcggcctcg accagctccc ggaccgtccc ggcgcgatga
     3001 tgggcgcccg cacggcggcg gcgcagctcg gctacatggt cggtgctgcc ggaggcggcg
     3061 gcgtgctggc gctgtggggc ttcggcacgc tgggcttcgt cctctgcgcc gggatggcgc
     3121 tgtcggctgt gctgctgacg ggcgtccacg acccctggag cgagcgccag cgagccggcg
     3181 cggcggaggc gctgcccgcg ccgttggtcg actgactggg cgaggacgcc gcggcggccg
     3241 atgcggggtt gagcgcgtcc tagccggcga atccttcacc gggttcgccg cagcagcgtg
     3301 ccggcacggc gaatgaaggg ttgtgggcgc tagagcacgc caatccttca tctgaccgtc
     3361 ccgggctcac acggccagga gagcgggcag gacgcgcatg tcgtcgaaga cctcgccccc
     3421 ggcggcggcg aggtcgtcgg cggcgaccag ccgggcgaag gcgagcacgc gcatgccggc
     3481 ggcgcggccg gcttggacgc cggggacggt gtcctcgacg accgccgtct cggaaggctg
     3541 gaagcccatc cggtcggccg cgtggagaaa gaggtcgggg gcgggctttc cgtgctggac
     3601 ttcggtcgcg ctgaagaggc gcccctcgaa gcgcggcagc aggtcggtga ggccgagcgt
     3661 gaggcgcatc cgctcgtgct cgccgctgga ggcgacgcag ctcggcaggt cgatcgcgtc
     3721 gagcgcctcg accaccccgg gcacgggacg caggtcgcgc ttccaggccg cggtcacgcc
     3781 ggcgcggtag cgatcgccga agtcgtcggg cggcggatgg ccgagcttgc gcccgacgat
     3841 ctcgaggcac gaggcccacg accgccccat gtactcggcg agcgcctgct cgggcgtcgt
     3901 cgggataccg agctcggtga tcaggtcggc cagcactacg ttcgccgccg gctcggtgtc
     3961 caccaggacg ccgtcgcagt cgaagatcac gctctcggcc acggcgggca ctgtaggcga
     4021 cggccggtag gctggccggc cagcaagccg acccccgaag caaaggactc gcgatggcga
     4081 tcgacttcga cgcggtacgc gccggcctcg agcaggcgat cccgttcaac aagcacctcg
     4141 ccctcgaggt cgtcgccgtc gccgacggca gcggctccgt tcggctgccc gacgacccgg
     4201 cgctgctcaa ccacgtcggc tcccagcacg cgggcgcgct gttctcggtc gccgaggccg
     4261 catcgggcgc cgcgttcgtc ggcgccttcg ccgagcggat gggcgagatc acgccgctcg
     4321 ccaagtccgc gagcatccag taccgcaagc tcgccaaggg cccgatcgtc gccaccggga
     4381 ccctctccaa ggacaaggcc tcgctgctcg agcgactcga cgccgacggc cgcgtcgagt
     4441 tcacgatcga cgtctcgctc agggacgccg acgacgtaga ggtggccacg gtcaccgtcg
     4501 actggcacgt ccgcaagaac ggctgaactc gtacggacgc cgggccactt ccggcggcat
     4561 gagcgccgtg atcggcccag ctcgagccgg tcctcttcga ctatccatgc cgggctgata
     4621 ccggcggcaa tgtcgccgta atcggcccag tctcgcggcc ggcgccccgc gtcagcgaag
     4681 ctgagcgagg ttcttggcgt ggatgtggcg ctggcgggcg ctgtcgcgac cgtccggcag
     4741 cttcggccgc tcgtcgccgc gctccagtgt gtctagatac tcgaggtcct cgtcgatcag
     4801 gcgcagcgcg gcggcacggg actgcggtac gccgtggccc gggaccaccg cctcggcctg
     4861 ctcgaccagc ggcgcaagac gggcgagcgt gtcgcggtag gcgccgatcg acccgcccgc
     4921 agagatccac gggatctcga ccggactgac gtagtcgccg gtgcagagca cgcccagcca
     4981 gggcgcccag accgccatgc cgtccggggt gtggccctcg gcggtggcga gctgcagctc
     5041 ctgatcgccc agctcgagct ggccgggcac cggcagcgac tggacctcgc cgagcgatag
     5101 cggccgccca cgctcgacgt agtgctcggc gtcggcgtcg cgcagctcgc gctgggcctc
     5161 tcccggccgc tcgcggatcc gccgcatcgt gccctccgac acgccgagcg cgagcccggg
     5221 aaacgccagc cgacccagca ggtggtcgaa gtcgccgtgc gtggccagca gcgcgttggg
     5281 ctcgaaccct gactgcgcca gcacgccggg cagcagctcg agctcgtccg ggaagtaggg
     5341 cgagtcgatc agcatcgcct cgccgcccgc gcgcagcgcg atcgcgttgg tctgccagag
     5401 caggctggtg acgacgatcg cgtcgctgtg gaccgagacg gcgcgcaccg cgccctcccc
     5461 tagaccaggc cgagcagctt cgccgcgtcg cggaaggtct tgacttcctc ggtcggcttc
     5521 ttctgaccgg actcgagctc gtagccgtgg cggtttaccc agatgaccgg gaccttcgac
     5581 ttcaggcacg gctcgacgtc ggtcgggtag ccggaggcga tgtggaccca gttcttcttg
     5641 cgcttctcga tccgccgcgc gcactccttg aagtgggccg ggtcgggctt gtagctgcgg
     5701 acctgctggg cggtcacgac gaggtcgaag tcactgcgga agtggcgccg cgtcgcgccg
     5761 agcagcttgt cgtcgatgtt cgacaggatc ccgagctcgt acttcttcgc gaaccgctcc
     5821 agctgggcgt tggtctcgcg gaacggctgc cagcgcggca cgctgtccgg caggaactgc
     5881 gcgcgcgagg actcgagacc ccagcccagc tcctcggcga cctggaccgc ggtccggcgc
     5941 agcacctcgg catacagctc gtaggagccg ctctgaatct tgcgctggat ctcgatgaag
     6001 cgcggcacca gcacgtcgcg gtcgatcgtg aagccgtcgc ggtcggcctc cttctggaac
     6061 gcgtcgtaga cccccgtctc ccagtcgatc agcgtgccgt agcagtcaaa ggtgacgaac
     6121 tggatgtcct tcggtaacgg cactggccct cctgaggctt gctgcggtgg ggaagcggcc
     6181 gggcattcta tgtcgcgcga ccgcagcgcg ctgggcgcct cccctgctgt agcctgccgc
     6241 gccggccgat ggacctcctc gagtaccagg gcaagcagct cttcgccagg cacggcatcc
     6301 ccgttccgca gggaaagccc gcccagaccg tcgacgaggc ggtcgccgcc gccgaggagc
     6361 tcggctatcc cgtcgtcgtc aaggcgcagg tgctggtcgg cggccgcggc aaggccggcg
     6421 gcgtcaagct cgccgccgac cgcggcgagg tcgagcagca cgccggcgcg atcctcggca
     6481 tggacatcaa gggccacacc gtccacgagc tctacatcga ggctgcgagc gacatcgacg
     6541 aggagtacta cgccgccttc gtcttcgacc gctccgcgaa gaagccgatg gcgatgttct
     6601 cgaagatggg cgggatggac gtcgaggcgg tcgccgagac cgaccccgag gcgatgcgca
     6661 cgctgcacgt cgacccgctg ctcggcttcc aggacttcca gggccgccgg ctcgccttcg
     6721 aggccggcat ccccgccgac gtgatccgcc cggtcggcgc gctgctcgcg aagctctacg
     6781 aggtcttcgt cgccgaggac gcgatgctcg tcgaggtcaa cccgctgctg atcacgaaat
     6841 cgcgcgaggc cgtggcgctc gacgcgaagg tgacgctcga cggcaacgcg ctcttccgcc
     6901 acccctacgc cgccgagttc ggcgacaagg gcgccgccga cccgcaggag aagatggcca
     6961 aggagcgcgg cctgacctac gtcaagctcg acggcaacat cggcatcctc ggcaacgggg
     7021 ccggcctggt gatgtcgacg ctcgacgtcg tcgcccaggc cggcggccag ccagccaact
     7081 tcctcgacgc cggcggcggg gccaaggccg aggagatcac gagcgcggtc gaggtgatcc
     7141 tctccaacga gaacgtctcc gccgtgctgt tcaacatctt cggcggcatc acccgctgcg
     7201 acgaggtggc gaacggcctg gtcgaggcgt tcaagcaggt caaccccgac gtcccgttcg
     7261 tggtccgtct cgacggcacc aacgacgagg aaggccgccg gatcctgcag gaggccaacc
     7321 tgtctggcct gtacgtcgag aagaccatgc tcgaggccgc cgagcgcgtc gtggctctgg
     7381 ccaaggaagg gggagccaag tgagcatcct catcgacaag gacaccaagc tctgcgtctc
     7441 gggcatcacc gggcgcgagg gcaccttcca cacgctcaac aacaagcgct acggcacgaa
     7501 cgtcgtcgcc ggcgtcacgc ccggcaaggc cgggcaggac gtcgagggca tcccggtgtt
     7561 caacacgttc gccaaggcgg tcgaggagac cggcgccaac accgcgatgg tgttcgtgcc
     7621 gccgcgcttc gcggccgact cgatcctcga ggccgaggac gccggcatcg agctgatcgt
     7681 ggcgatcacc gagggcatcc ccgcccacga cgagctgcgc gtctacaacc acctggcgcg
     7741 caatccggac gtgcggctcg tggggccgaa ctgcccgggc atcctctcgc ccggcaaggc
     7801 caacgtcggc atcatccccg ccgcgttctt cagcgagggc aacgtcggcg tcgtctctcg
     7861 ctcgggcacg ctgacctacc agatcggcaa tgagctggcc cagcgcggct tcggcaactc
     7921 gacgatcgtc ggcatcggcg gcgacccggt gccggggtcg agcttcatcg acgtcctcgc
     7981 ccgcttccag gaggacgacg agaccgagct gatcgtgatg gcgggcgaga tcggcggctc
     8041 ggccgaggag gagccggccg agttcatcgc cgagaacgtg acgaagccgg tggtcgccta
     8101 catcgcgggc ttcaccgccc cgccgggcaa gaccatgggc cacgccggcg cgatcgtctc
     8161 cggctcctcg ggcaccgccg cggcgaaggc cgaggcgctc gagtccaagg
//
LOCUS       DATABV010000130         6508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1727659, whole genome shotgun sequence.
ACCESSION   DATABV010000130 DATABV010000000
VERSION     DATABV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727659"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VHF88_05610"
     CDS             complement(<1..1044)
                     /locus_tag="VHF88_05610"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX2161282.1"
                     /translation="MLSKRTVRDLDLQGGERVLVRVDFNVPLEDGRVTDDARIRAALP
                     TIELLLDRGARPILCSHLGRPKDREPETSLKPASDRLRELVDAPVEQAAGVVGDDVRG
                     AVDALEPGAILVLENTRWEPGEKANDAELARELASYADLYVNDAFGAAHRAHATTAGV
                     AEHLPAVAGLLLEREVDTLDGIVESPERPLCVVLGGAKVSDKVALIDRFLDTADCVLI
                     GGAMCFSFFRAQGRPTGDSLVEEEGVELARKALEKADAGDGARLLLPLDLVLGDRFAA
                     DADRREHDGVDVPDGWMGLDVGSQTAEAYAREVRDAGTVFWNGPMGAFELEPFAAGTR
                     AVAEAVADAPGYTV"
     gene            complement(1050..2072)
                     /gene="gap"
                     /locus_tag="VHF88_05615"
     CDS             complement(1050..2072)
                     /gene="gap"
                     /locus_tag="VHF88_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX2161283.1"
                     /translation="MALKAGINGFGRIGRNVFRAAHERGADIDWVGVNDITDTATLAN
                     LLKYDSILGPFPGTVEATEDSLIVDGKQLRVFAERDPANLPWGDVGAEVVVESTGFFT
                     KRDDAAKHLAAGARKVIISAPATEPDVTLVLGVNDDAYDAGSHDVISNASCTTNCLAP
                     FASVLHDAIGIESGVMTTIHAYTGDQRLLDAPHKDPRRARAAAVNLIPTSTGAAKAIG
                     LVIPELNGKLNGIAVRAPVPTGSVVDLVFQASRDTSVEEVNAAVAERADSGALEGILQ
                     YTEDPIVSTDIVRSSFSSIFDSKLTMVIDGRMVKVVSWYDNEWGYSNRVVDLAERVLA
                     GAPAAA"
     gene            complement(2119..3006)
                     /gene="rapZ"
                     /locus_tag="VHF88_05620"
     CDS             complement(2119..3006)
                     /gene="rapZ"
                     /locus_tag="VHF88_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX2161284.1"
                     /translation="MQDFVIVTGLSGAGKSQAMATFEDAGYFCVDNLPPEMIAGLAEL
                     FQHEGSKVERAAIVSDVRGGAYFDGLVQVLDELAERDVPYRLLYLEASEDVLVNRYKE
                     TRRRHPLADGVASGGSVASAIGAEWDLLRPLRDRADVSIDTSELSAARLRKVVADKML
                     PRGKVGKLAVTFISYGFKHGTPRDADLLFDVRFLPNPHYEADLRDLTGLDAPVREYVQ
                     SSDGIEDFFDRLTPLLDYLLPQYEQEGKAHLAIGIGCTGGRHRSVVIAERLARQYGER
                     DDLLVDVVHRDAGKPPRPA"
     gene            3062..3808
                     /locus_tag="VHF88_05625"
     CDS             3062..3808
                     /locus_tag="VHF88_05625"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2161285.1"
                     /translation="MAEGGDPEQQERPDEQRPAVPPRERRAVGYLAAGGAILLAVVTI
                     GVALASLGGDGSGSAEPSSGPVARDRDEVHDLLPEGGTFPEPERVGSVREGARSADCE
                     LESFEAKSNLHISSPDETVRYRSDPPTSGKHHPVPAEDNAYAVSPDVRRIVHTLEHGR
                     VVIWFDEDLPAKARASLKAFYRDDSHHVLLVPDTTDMPYAVAATAWNREPGRYGTGRL
                     LGCREYSGDVFTALAAFRDRHRDRGPELVP"
     gene            complement(3805..5679)
                     /gene="uvrC"
                     /locus_tag="VHF88_05630"
     CDS             complement(3805..5679)
                     /gene="uvrC"
                     /locus_tag="VHF88_05630"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX2161286.1"
                     /translation="MPDAVELSRAERVAEQRRALPDAPGVYLFKDERGTVVYVGKAKS
                     IRKRVGSHFSKPGNRSALEMTSSVAEIEFMATETEAEALLAEQEFIKRHRPRFNVRLR
                     DDKSYPYIGISLDEDYPRVYFTREKHRRNRAYFGPFSNAKRVRETLDLLGKVFQHRTC
                     EGREPGRQSGSPCLDYFIKRCQAPCVGYVSKEEYRENVDAIVAFLSGRYREIEQDLER
                     SMRLAAADQEFEAAAHFRNRLRAVRSLLERQRIANESVGTLDAIAVAVEEADANAQVF
                     QVRDGVLADRQSFYLENAGERDEADVLEEFVLQYYASSMSIPPQVIVPRGGADCALLA
                     EALGERRGAPVEVRSAERGDKRRIFELAQRNARLALDQDRLRSEHRRSRRIEALEGLQ
                     RELAMPALPMRIECFDISNLGETHTVASMVVFEGGAPKKSDYRRFRIRGAAQDDFAAM
                     SEVLRRRVAQFRSQREISPHEKQHDASFAALPALIVIDGGKGQLSAGLPELQELRELG
                     TTVVSLAKRVEEVFMPGRSAPVMLPHDRAPLQLLQRVRDEAHRFAIEHHRLRRDKAMT
                     RSVLDDIPGVGPTRKRAILNHFGTPERVLAASREELECVPGLPGKVAREIHQHLHRVD
                     "
     gene            complement(5710..5982)
                     /locus_tag="VHF88_05635"
     CDS             complement(5710..5982)
                     /locus_tag="VHF88_05635"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX2161287.1"
                     /translation="MCRNIRTLHNFAPPASEEEIRDAALQYVRKVSGSTKPSKANQEV
                     FDRAVEEVAEATRNLLDGLVTSAPPKDREVEAAKHRERSAKRFATA"
     gene            6019..>6508
                     /locus_tag="VHF88_05640"
     CDS             6019..>6508
                     /locus_tag="VHF88_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161288.1"
                     /translation="MHGERLRLWALAITAVAILLIAAAPAQAAAPRDGWGFAIADDDW
                     EWDDPGHLPSLGDGFARLAPKAFRFQMIWNAVDYSWHMERARALIARARQQDVEQIVV
                     TFKKSVEPDVDPVCGPQPPAECYAAHVGEAVRQLASEVDVWGPANEPNLGETWLPGVP
                     GAQ"
BASE COUNT          916 a         2239 c         2363 g          990 t
ORIGIN      
        1 gaccgtgtag ccgggtgcgt cggcgaccgc ctcggcgacc gcgcgcgtgc cggcggcaaa
       61 cggctccagc tcgaaggcgc ccatcggacc gttccagaag accgtcccgg cgtccctgac
      121 ctcgcgggca taggcctcgg cggtctgcga gccgacgtcg aggcccatcc agccatccgg
      181 cacgtcgacg ccgtcgtgct cgcgccggtc ggcgtcggcc gcgaagcggt cgccgagcac
      241 gaggtccagc ggcagcagca gtcgcgctcc gtctccggca tccgccttct cgagcgcctt
      301 gcgcgccagc tcgacgccct cctcctcgac gagcgagtcg ccggtcggcc ggccctgggc
      361 acggaagaag ctgaagcaca tcgccccgcc gatcaggacg cagtcggcgg tgtcgaggaa
      421 gcggtcgatc agcgcgacct tgtccgacac cttcgccccg ccgaggacga cgcagagcgg
      481 gcgctcgggg ctctcgacga tcccgtcgag cgtgtcgacc tcccgctcga gcagcagccc
      541 cgcgacggcc ggcaggtgct cggcgacgcc ggcggtcgtg gcgtgggcgc ggtgggcggc
      601 gccgaaggcg tcgttgacgt agaggtccgc gtaggaggcc agctcgcgcg ccagctcggc
      661 gtcgttggcc ttctcccccg gctcccagcg cgtgttctcg agcacgagga tcgctcccgg
      721 ttcgagcgcg tcgactgcgc cccgcacgtc gtcgccgacc acgccggccg cctgctcgac
      781 cggagcgtcg acgagctcgc gcaggcggtc cgacgccggc ttcagcgacg tctccggctc
      841 tcggtccttc ggtctgccga ggtgcgagca gaggatcggc cgcgcgccgc ggtcgagcag
      901 cagctcgatc gtcggcaggg cggcccttat ccgcgcgtcg tcggtgaccc gcccgtcctc
      961 gagcgggacg ttgaagtcca cgcgaacgag cacgcgctcg ccgccctgga ggtccaggtc
     1021 gcggaccgtg cgcttgctga gcactacgac taggccgcag ccggcgcgcc ggcgagcacg
     1081 cgctccgcga gatcaaccac gcggttcgag tagccccact cgttgtcgta ccaggagacg
     1141 accttgacca tccggccgtc gatcaccatc gtcagcttcg agtcgaagat cgacgagaac
     1201 gacgagcgga cgatgtcggt cgagacgatc gggtcctcgg tgtactggag gatcccctcg
     1261 agcgcgccgc tgtcggctcg ctcggccacc gccgcgttga cctcctcgac cgaggtgtcg
     1321 cgcgacgcct ggaagacgag gtcgacgacc gatccggtcg gcacgggagc ccgcaccgcg
     1381 atgccgttca gcttcccgtt gagctcgggg atcaccaggc cgatcgcctt cgcggcgccg
     1441 gtcgaggtcg ggatcaggtt gacggcggcg gcacgcgcac gacgcgggtc cttgtgcggc
     1501 gcgtcgagca ggcgctggtc gccggtgtag gcgtggatcg tggtcatcac gccggactcg
     1561 attccgatcg cgtcgtgcag gacgctggcg aacggcgcga ggcagttggt cgtgcaggag
     1621 gcgttggaaa tcacgtcgtg cgacccggcg tcgtaggcgt cgtcgttgac gcccagcacg
     1681 agcgtcacgt cgggctcagt cgcgggggcg gagatgatca ccttgcgcgc gccggccgcg
     1741 aggtgcttgg cggcgtcgtc gcgcttggtg aagaagccgg tcgactcgac gacgacctcg
     1801 gcgccgacgt caccccatgg aaggttcgcg ggatcgcgct ccgcgaagac gcgcagctgc
     1861 ttgccgtcga cgatcaggct gtcctcggtc gcctccaccg tgccggggaa cggccccagg
     1921 atcgagtcgt acttgagcag gttggcgagc gtcgccgtgt cggtgatgtc gttcacgccg
     1981 acccagtcga tgtccgcgcc acgctcgtga gccgctcgga agacgttgcg gccgatgcgg
     2041 ccgaagccgt tgattccggc cttcaaggcc attccagggg tcctttcctt cggaagacgg
     2101 gatctctgaa tctaccggtc aggcggggcg aggcggcttg ccagcgtcgc ggtggacgac
     2161 gtcgacgagc aggtcgtcgc gctcgccgta ctggcgcgcc agccgctcgg cgatgacgac
     2221 cgagcggtgg cggccgccgg tgcagccgat gccgattgcg aggtgggcct tgccctcctg
     2281 ctcgtactgc ggcagcaagt agtcgagcag cggggtcagg cggtcgaaga agtcctcgat
     2341 gccgtcggag gactgaacgt actcgcgcac aggcgcgtcg agcccggtca ggtcacgcag
     2401 gtcggcctcg tagtgcgggt tcggcaggaa gcgcacgtcg aacagcaggt cggcatcgcg
     2461 cggggtgccg tgcttgaagc cgtagctgat gaaggtgacc gcgagcttgc caaccttgcc
     2521 gcgcggcagc atcttgtcgg cgacgacctt tcgcagtcgg gcggcgctca gctcgctggt
     2581 gtcgatcgag acgtcggccc ggtcgcgcag cggtcgcagc agatcccact cggcgccgat
     2641 cgcgctggcc accgagccgc ccgaggcgac gccgtcggcc agcggatggc ggcggcgcgt
     2701 ctccttgtag cggttgacca gcacgtcctc gctcgcctcc aggtagagga gccggtaggg
     2761 gacgtcgcgc tcggcgagct cgtcgagtac ctggacgagc ccgtcgaagt acgccccgcc
     2821 ccgtacgtcg gacacgatcg ccgcccgctc gaccttcgac ccctcgtgct ggaacagctc
     2881 cgcgagtccc gcgatcatct ccggcggcag gttgtcgacg cagaagtagc cggcgtcctc
     2941 gaacgtcgcc atcgcctggc tcttgccagc tccggagagc ccggtgacga tcacgaagtc
     3001 ctgcacgcat gggtctttgt agcggttgcg ctagaccgac ccagctactg ttcgccccgc
     3061 gatggccgag ggaggggatc cagagcagca ggagaggccg gacgagcagc gacccgcggt
     3121 gccgccacgg gagcgtcgcg cggtcggcta tctcgccgcg ggcggggcga tcctgctcgc
     3181 cgtggtgacg atcggcgtcg cgctcgcgag cctgggcggc gacggtagcg ggagcgccga
     3241 gccgtcctcc ggcccggtcg cccgcgaccg cgacgaggtc cacgacctgc ttcccgaggg
     3301 cggcacgttc cccgagccgg agcgcgtcgg cagcgtgcgc gagggcgcac ggtcggcgga
     3361 ctgcgagctc gagagcttcg aggcgaagag caatctgcac atctcgagcc cggacgagac
     3421 cgttcgctac cggtccgacc cgccgacatc gggcaagcac catcccgtgc cagccgagga
     3481 caacgcctat gccgtatcgc ccgacgtccg gcggatcgtc cacacgctcg agcacggccg
     3541 cgtcgtgatc tggttcgacg aggacctgcc tgcgaaggcg cgcgcgagcc tcaaggcctt
     3601 ctatcgcgac gactcccacc acgtgctgct cgtccccgac accaccgaca tgccgtacgc
     3661 ggtcgcggcg accgcctgga accgcgagcc gggccggtac ggcaccggcc gcctgctcgg
     3721 ctgccgcgag tacagcggcg acgtcttcac cgcactcgcc gccttccgcg accgccaccg
     3781 cgaccgcggc ccggagttgg tgccttagtc gacgcggtgc aggtgttggt ggatctcgcg
     3841 ggcgaccttg ccgggtaggc cgggaacgca ctcgagctcc tcgcggctgg cggcgaggac
     3901 gcgctcgggc gtgccgaagt ggttgaggat cgcgcgcttg cgggtcgggc cgacgccggg
     3961 gatgtcgtcg aggacggagc gcgtcatcgc cttgtcgcga cgcaggcggt ggtgctcgat
     4021 cgcgaagcgg tgggcctcgt cgcgaacgcg ctgcagcagc tgcagcgggg cccggtcgtg
     4081 gggaagcatg accggagcgg agcggcccgg catgaagacc tcctcgacgc gcttggcgag
     4141 gctgacgacc gtcgtgccga gctcgcgcag ctcctgcagc tcgggcagcc cggcggagag
     4201 ctggcccttg ccgccgtcga tcacgatcag ggcaggcagc gcggcgaagc tggcgtcgtg
     4261 ctgcttctcg tgcggcgaga tctcgcgttg ggagcggaac tgggcgacgc ggcggcgcag
     4321 cacctcggac atcgcagcga agtcgtcttg ggcggcgccg cggatccgga agcggcgata
     4381 gtcggacttc ttcggcgccc cgccctcgaa gaccaccatt gacgcgaccg tgtgggtctc
     4441 gccgaggttc gagatgtcga agcactcgat tcgcatcggc agcgccggca tggcgagctc
     4501 gcgctgcaag ccctcgagcg cctcgatccg gcgcgagcgg cggtgctcgg agcgcagccg
     4561 gtcctggtcg agtgccaggc gggcgttgcg ctgagcgagc tcgaagatcc gccgcttgtc
     4621 gccgcgctcg gccgagcgga cctcgaccgg cgcgccgcgc cgctcgccga gcgcctcggc
     4681 gagcagggcg caatcggcac cgccgcgcgg gacgatcacc tgcggcggga tcgacatcga
     4741 gctggcgtag tactgcagga cgaactcctc gagcacgtcg gcctcgtcgc gctcgccggc
     4801 gttctcgagg tagaagctct ggcggtcggc cagcacgccg tcgcggacct ggaagacctg
     4861 tgcgttggcg tcggcctcct cgacggcgac ggcgatcgcg tcgagcgtcc cgaccgactc
     4921 gttggcgatc cgctggcgct cgagcagcga tcgcacggcg cgcagccggt tgcggaagtg
     4981 cgcggccgcc tcgaactcct ggtcggcggc ggcgagccgc atgctgcgct cgaggtcctg
     5041 ctcgatctcg cggtaacggc cggacaggaa agcgacgatc gcgtcgacgt tctcgcggta
     5101 ctcctccttc gacacgtagc cgacgcaggg cgcctggcag cgcttgatga agtagtcgag
     5161 gcaagggctg ccggactggc gccccggctc tcgcccctcg caggtgcggt gctggaagac
     5221 cttgccgagc aggtcgagcg tctcacgcac ccgcttcgcg ttcgagaagg gtccgaagta
     5281 ggcgcggttg cgacggtgct tctcacgtgt gaagtagacg cgcggatagt cctcgtcgag
     5341 cgagattccg atgtaggggt acgacttgtc gtcgcgcagg cggacgttga agcgcggccg
     5401 gtggcgcttg atgaactcct gctcggctag cagcgcctcg gcctccgtct cggtcgccat
     5461 gaactcgatc tccgcgaccg aggaggtcat ctcgagcgcc gagcggttgc ccggcttgga
     5521 gaagtgcgag cccacccgct tgcggatcga cttcgccttg ccgacgtaga cgaccgtgcc
     5581 gcgctcgtcc ttgaacaggt agacgcccgg cgcgtcgggc agcgcgcgcc gctgctcggc
     5641 gacgcgctcg gcacggctca gctcgacggc atcgggcatc ccgtcgagga tagggccggt
     5701 cagctgcggc taagcggtcg cgaagcgctt tgcggaccgc tcgcggtgct tggcggcctc
     5761 aacctcgcgg tccttcggcg gggcgctggt cacgaggccg tcgagcaggt tgcgggtcgc
     5821 ctcggcgacc tcctcgacgg cgcgatcgaa gacctcctgg ttggccttcg acggcttcgt
     5881 cgagccgctg actttgcgga cgtactgcag tgcggcgtcg cggatctcct cctcgctggc
     5941 ggggggcgcg aagttgtgca gggttcggat gtttcgacac atgcggtcaa gcgtagagca
     6001 gcccctatcg tggagatcgt gcatggggag cgtttgcggc tgtgggctct ggcgatcaca
     6061 gccgtggcga ttctgctgat agccgccgcg ccggctcagg ccgccgcgcc gcgcgacggc
     6121 tggggcttcg cgatcgccga cgacgactgg gagtgggacg acccggggca tcttccctcg
     6181 ctcggcgacg gcttcgcccg tctcgcgccg aaggccttca ggttccagat gatctggaac
     6241 gcggtcgact actcctggca catggagcgg gcgcgcgcgc tgatcgcccg cgcgcgccag
     6301 caggacgtcg agcagatcgt cgtcaccttc aagaagagcg tcgagccgga cgtggatccg
     6361 gtctgcggcc cgcagccgcc agccgagtgc tatgccgccc acgtcggcga ggcggtgcgg
     6421 cagctcgcca gcgaggtcga cgtctggggc ccggcgaacg agccgaacct cggcgagacg
     6481 tggctgccag gagtccccgg cgcccagc
//
LOCUS       DATABV010000131         5873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2370773, whole genome shotgun sequence.
ACCESSION   DATABV010000131 DATABV010000000
VERSION     DATABV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370773"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VHF88_05645"
     CDS             <1..436
                     /locus_tag="VHF88_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161289.1"
                     /translation="FDPEGITGNQEIPFAKSLPDYIMRWLASRFLDVDVQEELGIMTA
                     EVKARKAAEEAALRGDTAGPPNGNGSNGHGNGNGSSGDGAPSASAPAPAAAALTDEPP
                     VAAPAKITGLDLGPACSQCGGMMQRTGTCYTCSSCGNNTGCG"
     gene            457..909
                     /locus_tag="VHF88_05650"
     CDS             457..909
                     /locus_tag="VHF88_05650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2161290.1"
                     /translation="MVAALETERLVGEPASAEHRDFVAELFGEPEVARWIWPEGREGS
                     DGAGPRTPAQAEELLQRFLADWDSCGFGWWFLRERAGGRLVGDVVLQRAEVEGEQVVE
                     VGWMMLPAFCGRATRPRPRGRRSRTASDRPAWTRWSPSRCRTTSRHAG"
     gene            complement(1134..1772)
                     /locus_tag="VHF88_05655"
     CDS             complement(1134..1772)
                     /locus_tag="VHF88_05655"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2161291.1"
                     /translation="MSSGSTSPAPTDRAPTGSAEPAPPTRGAARRRALLDATVRVIGR
                     GGIAAVDHRAVAAEAGVPLGSTTYYFKSKHDMVARALEHVADREAERLRVESERGALD
                     VGPDLLPERLADVVIEVWAGDRVTLLAQYELYLESARRPDLRPAAERWDAAYRDFLEH
                     GLELLGAPDPERRAHLLCAALDGLLLDHVATGSDESDLRSLVVELIERVAAP"
     gene            complement(1738..2061)
                     /locus_tag="VHF88_05660"
     CDS             complement(1738..2061)
                     /locus_tag="VHF88_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX2161292.1"
                     /translation="MSPAVLLALAIVSEVLGTVLLRLSDGMSKLVPSVGMLAAYGVSV
                     YLLALVLKHIDIGLTYAIWAGVGTAMVAVIGVAAWGEPMGLVKVLSIGAVIVGVIGLN
                     LSGAH"
     gene            complement(2126..2707)
                     /locus_tag="VHF88_05665"
     CDS             complement(2126..2707)
                     /locus_tag="VHF88_05665"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX2161293.1"
                     /translation="MEPEDPTEASVRASDAEREQAVDRLKGAAAEGRLTLEELADRTE
                     AAYAATVRGELERVVADLPAAPARTSAVPAKTRRTFVGIMGGDSIHGPIRLADRCTIV
                     NVMGGVDLDLSRATIEGGELTLRIFSLMGGSTIRVPPGVHVERTGFSLMGGDSIRPPD
                     DPPPSPDAPVIRIRSFNLMGGSNVRPPRRRDRS"
     gene            2793..3263
                     /gene="greA"
                     /locus_tag="VHF88_05670"
     CDS             2793..3263
                     /gene="greA"
                     /locus_tag="VHF88_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX2161294.1"
                     /translation="MSDTTKLTAAAHAHLTEEIERLEGEGRRAIAARIKTAREWGDLK
                     ENAEYHAAKEEAAMLEAKIAGLRDQLRSAEIVETAAGDVAGMGTTVTYKDEKSGKELS
                     HKLVPKVEADPSAGLISIDSPVGKALAGAKVGDAATLETPSGMRTMRVLAVDHS"
     gene            complement(3269..4351)
                     /locus_tag="VHF88_05675"
     CDS             complement(3269..4351)
                     /locus_tag="VHF88_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2161295.1"
                     /translation="MPSSLITGFPGFIGRRLVRKLLEQDRRARIVALVEPRMLDAARA
                     AAAEIDPARIEIVAGDIADRRLGLDDAEYARLASDVRRVYHLAAIYDLAVPIELAQRV
                     NVDGTGNVLELCLAAKKLERLVYISTAYVAGKRRGVVYEHELLMGQQFKNHYESTKFQ
                     AEVWVRQLLDRIPTTILRPAIVVGDSRTGETQKFDGPYYLLRAIAQAARQGRPPVQIG
                     ASDATFNVVPLDFVVDAIAVAGVDPATVGETLHLVDPSPVTTRELVELLSQQYAGRAP
                     RGKIPPALAQGGLRFKPIRKLFSDTPAESIAYLNHAVTFDTRRAIDLLTPHGLAPPPF
                     ASYAGPMVEFFRANEHDPALAPRAAA"
     gene            4436..5338
                     /locus_tag="VHF88_05680"
     CDS             4436..5338
                     /locus_tag="VHF88_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2161296.1"
                     /translation="MPAATIPAPPEIPGAEQRSVTIDTHDVGPLGIHLYDAGDGPPVL
                     LLHGWPQDAACWRHVIPRLADRHRLIAPDLRGFGRSDAPDSGYDGMTFGADAIALLDA
                     LEIERAHVIGHDWGGFAAFAAGIAAPSRIASMVVLNTIPPWVEPSPRLVLELWRTSYA
                     FALAAFGERIVRGRPDVIARMLRADRVHDGITRADAEAYAARLQRPESARATALLYRS
                     YTRSFKAVMVERRFKDLRLSVPTRFLFGTNDMAVPRQLLNGVERHCDDLVVEYVADSG
                     HFIQEEKPGLVAEQAAELFARYPA"
     gene            5439..5843
                     /locus_tag="VHF88_05685"
     CDS             5439..5843
                     /locus_tag="VHF88_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161297.1"
                     /translation="MRSIGAAIALAALLTAGLAALANAATTSQSPPLREFEGRVVSID
                     RGARTFRLHDSERGTKRIKVTRGTRYERVAGLAGLKVGQRFIEVKTRRKVRGAWIAIE
                     VERSGGGGEHGGRDDDDDDRDDDSGRGRGGDD"
BASE COUNT          871 a         2077 c         2160 g          765 t
ORIGIN      
        1 cttcgacccc gaggggatca ccggcaacca ggagatcccg ttcgcgaagt cgctgcccga
       61 ctacatcatg cgctggctcg cctcgcgctt cctcgacgtc gacgtccagg aggagctcgg
      121 gatcatgacc gcggaggtca aggcgcgcaa ggcggccgag gaggccgcgc tgcgcggcga
      181 caccgcgggc ccgcccaacg gcaacggctc gaacgggcac ggcaacggga acggcagctc
      241 cggcgacggc gccccgtccg cgagcgctcc cgccccagcc gccgcggcgt tgaccgacga
      301 gccgccggtc gccgctccgg ccaagatcac cggcctcgac ctcggtcccg catgctccca
      361 gtgcggcggc atgatgcagc gcaccggcac ttgctacacg tgctcgagct gcggcaacaa
      421 caccggctgc ggctgagccg gcgctaggcg ccgccgatgg tggcggcgct ggaaaccgag
      481 cggctggtcg gcgagccggc gtccgcggag catcgcgact tcgtcgcgga gctcttcggg
      541 gagccggagg tcgcgcgctg gatctggccc gaggggcgcg agggatccga cggcgcggga
      601 ccgaggacgc cggcgcaggc ggaggagctg cttcagaggt tcctcgccga ctgggacagc
      661 tgtgggttcg gttggtggtt cctgcgcgag cgcgccggcg ggcggctcgt cggcgacgtc
      721 gtcctccagc gagccgaggt cgagggcgag caggtggtcg aggtcggctg gatgatgctg
      781 ccggccttct gcggacgggc tacgcgaccg aggccgcgcg ggcggcgctc gcgcacggct
      841 tcggaccggc cggcctggac gaggtggtcg ccttcacgct gccgcacaac atcgcgtcac
      901 gccgggtgat ggagaagctt gggatgtcct acgaccgcga cttcacccgc gccggcctcc
      961 cccacgtcct ctaccgcctc gagcgtcccc gccgctgacc gccacgctcg atgatggttt
     1021 gtgacgtcca gacgatgcca acccctcagc gggtcgggct cacggcgccg cgacgccgcc
     1081 gatgtggcct tgtgacgtca gggcgatgcg aatccttcag cgggcgacga gcgtcacggc
     1141 gcggcgacgc gctcgatgag ctcgaccacg agcgaacgga ggtcggactc gtcgctgccg
     1201 gtggcgacgt ggtcgagcag gagtccgtcg agtgcggcgc agagcaagtg ggcgcggcgc
     1261 tccgggtccg gagcgccgag cagctcgagt ccgtgctcga ggaagtcgcg ataggcggcg
     1321 tcccagcgct cggcggccgg gcgcaggtcg gggcggcggg cggactcgag gtagagctcg
     1381 tactgggcga gcagggtgac gcggtcgccg gcccagacct cgatgacgac gtcggcgagc
     1441 cgctccggca ggaggtcggg gccgacgtcg agcgcgccgc gctccgactc gacgcgcagg
     1501 cgctcggcct cgcggtcggc gacatgctcg agcgcacggg cgaccatgtc gtgcttggac
     1561 ttgaagtagt aggtggtcga gccgagcggg acgccggcct cggcggcaac cgcgcggtgg
     1621 tcgacggcgg cgatgccgcc gcgcccgatc acgcgcaccg tcgcgtcgag cagcgcccga
     1681 cggcgcgcag caccgcgggt cggcggcgcg ggctcggcgg agcccgtcgg cgcccgatca
     1741 gtgggcgccg gagaggttga gcccgatgac accgacgatc accgccccaa tgctgaggac
     1801 cttgacgagc cccatcggct ccccccaggc ggcgacgccg atcacggcga ccatcgccgt
     1861 cccgaccccg gcccagatcg cataggtcag gccgatgtcg atgtgcttga gcaccagggc
     1921 gagcaggtag accgacaccc cgtaggcggc gagcatgccg acagaaggga cgagcttcga
     1981 catcccgtcg gagagccgca gcaggacggt cccgagcacc tccgagacga tggcgagcgc
     2041 gagcaggact gcgggagaca tcggatcact tcctcagtag tacaggagta catgtacgac
     2101 tgtacacctt tgacgcccgc ggcgtctagg agcgatcgcg ccggcgcggc gggcggacgt
     2161 tgctgccgcc catcaggttg aagctgcgaa tccggatcac cggcgcgtcc ggcgacggcg
     2221 gcggatcgtc aggcggcctg atgctgtcgc cacccatcag cgagaagccg gtccgctcga
     2281 cgtggacgcc cggcgggacg cggatcgtcg acccgcccat cagcgagaag atccgcagcg
     2341 tcagctcacc gccctcgatc gtcgcccgcg agaggtcgag gtcaacgccg cccatcacgt
     2401 tgacgatcgt gcagcggtcg gcgagccgga tcggtccgtg gatcgagtcg ccgcccatga
     2461 tcccgacgaa ggtgcggcgc gtcttggccg gcaccgccga ggtccgcgcc ggcgcggccg
     2521 ggaggtctgc caccacccgc tcgagctcgc cgcgcacggt cgccgcgtag gcggcctcgg
     2581 tgcgatcggc gagctcctcg agcgtcaacc gtccctcggc cgcggcgccc ttgagccgat
     2641 cgacggcctg ctcgcgctcg gcgtccgacg cgcggacgct cgcctcggtg ggatcctccg
     2701 gctccacgcc gcgtgacgct agcgcctctc gcgggcaccc ggctgcccgc ccgtcgagac
     2761 cgcacaatcg accatctagg gttcggggtc tcatgtccga caccaccaag ctcaccgccg
     2821 ccgcccacgc ccacctgacc gaggagatcg agcgcctcga aggggagggc cgccgcgcga
     2881 tcgccgcgcg gatcaagacc gcgcgcgagt ggggcgacct caaggagaac gccgagtacc
     2941 acgcggccaa ggaggaggcc gcgatgctcg aggccaagat cgccggcctg cgcgaccagc
     3001 tccgctcggc cgagatcgtc gagacggccg ccggcgacgt cgccggcatg gggacgaccg
     3061 tcacttacaa ggacgagaag agcggcaagg agctcagcca caagctcgtt ccgaaggtcg
     3121 aggcggaccc cagtgccggc ctgatctcga tcgactcacc cgtcggcaag gcgctcgccg
     3181 gcgccaaggt cggcgacgcc gcgacgctcg agacgccctc cggcatgcgc acgatgcgcg
     3241 ttctcgccgt cgaccactcc tagctgcgct acgccgccgc gcgcggcgcg agcgccgggt
     3301 cgtgctcgtt ggcgcggaag aactcgacca tcgggcccgc gtagctggcg aagggcggcg
     3361 gggcgaggcc gtgcggggtc agcagatcga tggcgcggcg cgtgtcgaag gtgaccgcgt
     3421 ggttgaggta ggcgatcgac tccgctggcg tgtcggagaa gagcttgcgg atcggcttga
     3481 agcgcaggcc gccctgggcc agcgccggcg ggatcttgcc gcgcggggcc cggccggcgt
     3541 actgctgcga cagcagctcg acgagctcgc gcgtggtgac ggggctggga tcgacgaggt
     3601 ggagggtctc gccgaccgtc gccggatcga cgccggcgac ggcgatcgcg tcgacgacga
     3661 agtcgagcgg gacgacgttg aaggtggcgt ccgaggcgcc gatctggacc ggcggccgcc
     3721 cctggcgcgc cgcctgggcg atcgcgcgca gcaggtagta cggcccgtcg aacttctgcg
     3781 tctcccccgt gcgcgagtcg ccgaccacga tcgccgggcg caggatcgtc gtcggtatcc
     3841 gatcgagcag ctgacggacc cagacctcgg cctggaactt cgtcgactcg taatggttct
     3901 tgaactgctg gcccatcagc agctcgtgct cgtagacgac gccccggcgc ttgccggcga
     3961 cgtacgccgt gctgatgtag acgagccgct ctagcttctt cgccgcgagg cagagctcga
     4021 gcacgttgcc ggtgccgtcg acgttgacgc gctgggcgag ctcgatcggc accgcgaggt
     4081 cgtagatcgc cgcgaggtgg tagacgcgac ggacatcgct cgcaaggcgc gcgtactcgg
     4141 catcatcgag tcccaggcgc cggtcggcga tgtcgcccgc cacgatctcg atgcgagccg
     4201 gatcgatctc cgcggcggcg gcgcgcgccg cgtcgagcat ccgcggctcg acgagcgcga
     4261 cgattcgcgc gcggcgatcc tgctcgagca gcttgcggac gagacggcgg ccgatgaagc
     4321 ctgggaaacc ggtgatcagc gaggagggca tgcggcggaa cgctaaggct cgggcgccga
     4381 tcgcgctgtg cgatcgctcc ggacgcgcgc cggcctgccg gtagggtcgg ctccgatgcc
     4441 cgccgcgacg atccccgctc cacccgagat ccccggggcc gagcagcgca gcgtgacgat
     4501 cgacacgcac gacgtcggtc cgctcggcat ccacctgtac gacgccggcg acggcccgcc
     4561 ggtgctgctg ctccacggct ggccgcagga cgccgcctgc tggcggcacg tgatcccgcg
     4621 cctcgccgac cgccaccggc tgatcgcccc ggacctgcgc gggttcggcc gcagcgacgc
     4681 gccggacagc ggctacgacg gcatgacctt cggcgccgac gcgatcgcgc tgctcgacgc
     4741 gctcgagatc gagcgcgccc acgtgatcgg ccacgactgg ggcggcttcg cggcgttcgc
     4801 ggccggtatc gcggcgccat cgcggatcgc cagcatggtc gtcctcaaca ccatcccccc
     4861 gtgggtcgag ccgtcgccgc ggctggtgct cgagctgtgg cgcacctcct acgcgttcgc
     4921 gctcgcggcc ttcggcgagc ggatcgtccg cggcaggccc gacgtgatcg cgcgcatgct
     4981 gcgcgccgac cgcgtccacg acggcatcac gcgcgccgac gccgaggcct atgcggcgcg
     5041 tttgcagcgg ccggagagcg cgcgcgccac cgcgctgctc taccgcagct acacgcgctc
     5101 gttcaaggcg gtgatggtcg agcggcgctt caaggacttg cgactcagcg tcccgacccg
     5161 cttcctgttc ggcaccaacg acatggcggt cccgcgccag ctgctcaacg gggtcgagcg
     5221 ccactgcgac gacctcgtcg tcgaatacgt cgcggactcc gggcacttca tccaggagga
     5281 gaagccgggc ctggtggccg agcaggcggc tgagctgttc gcgcgctacc cagcctgagt
     5341 gccggtcgcc tttaccaact cctaacccgc cgctgggccc tggctggacg gtccgcgggc
     5401 atggtcgtgg aagagcccac gactgagagg tgatcgagat gagaagcatc ggagcagcga
     5461 tagccctggc cgccctgttg accgccggcc tggcggcgct ggcgaacgcc gccacgacga
     5521 gccagagccc gccgctgcgc gagttcgagg gtcgcgtagt gtcgatcgac cgcggcgccc
     5581 gaaccttccg gctgcatgac agcgagcgcg gcacgaagcg catcaaggtc acgcgcggca
     5641 cccgctacga gcgcgtcgcc ggactggccg ggctgaaggt cggccagcgc ttcatcgagg
     5701 tcaagacacg gcgcaaggtc cgcggcgcct ggatcgcgat cgaggtcgag cggtccggcg
     5761 gcggcggcga gcacggcggc cgcgacgacg atgacgacga ccgcgacgac gacagcggtc
     5821 gcggtcgcgg cggcgacgac tgagcggtcc cctggcgggg gtagggtggc tcc
//
LOCUS       DATABV010000132         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_845298, whole genome shotgun sequence.
ACCESSION   DATABV010000132 DATABV010000000
VERSION     DATABV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845298"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VHF88_05690"
     CDS             complement(<1..675)
                     /locus_tag="VHF88_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX2161298.1"
                     /translation="MEHRFTGPSYTVGVEEELMIVDAETLDLANSIEGLLTDLEGHDA
                     EGEVKPELMESVCEVATTPCADTAAAGRQLDALRRLVMGAAAERGLAIGAAGTHPFAM
                     WEDQRIVSRPRYRELVAGLQWVARQELIFGIHVHVGVDDPDKAIHIANGMRVHLPILL
                     ALSANSPFWRADATGLLSSRTPIFRAFPRVGIPPRYDDWADWEQRIDFMVQSKAIPDY
                     TYLWYDV"
     gene            769..1692
                     /locus_tag="VHF88_05695"
     CDS             769..1692
                     /locus_tag="VHF88_05695"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEX2161299.1"
                     /translation="MSLGYDGKLYILAFDHRGSFQKKMFGIEGDPTPEQTATIADAKH
                     LIFEGMAKAADRGADPHASGALVDEQFGGDIPRDAKARGLKLAMPVEASGRNDFEFEY
                     GDAFGEHILKFDPDFSKVLVRLNPDGDAEMNETQLGKLKRLSDWLKQNDRKFLFELLV
                     PAEPAQLESVGGDTDRYDAELRPDLMRRAIEQIQDAGIDVDIWKIEGVDEQADCEMLA
                     EVTRRDGRDGVVCVLLGRGASDAKVDHWLRQAAPVDGFVGFAIGRSIWWDALKGFLDG
                     SIAREDASDQIASNYLRFVEVYEGAEQGAHV"
     gene            1703..1942
                     /locus_tag="VHF88_05700"
     CDS             1703..1942
                     /locus_tag="VHF88_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161300.1"
                     /translation="MRMRQSLHQFEAAFREEAADSVARRERLRREAAQRSRARRVERV
                     HKHGTLRFIGLIATILLTTAIVTWAMFQVLASVAG"
     gene            1964..2545
                     /locus_tag="VHF88_05705"
     CDS             1964..2545
                     /locus_tag="VHF88_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161301.1"
                     /translation="MARRALKPQLNQIRTWVRQGRTDAWIAHQLEVSVKDVTNFKSEH
                     DLEAGETEGVETDLFGPRVDEEEIDLRAEDEALVAAALEEEASRQSEQEDEEEPTDEE
                     GEAREGEESEESRSRRRRGRRGGRGRKRGGRKKALEGTFDHGEDGYGLWLDPAIQDDP
                     LYAEHWAGHRPVTVTVEAERIVIERLTVSSDGE"
     gene            complement(2555..2866)
                     /locus_tag="VHF88_05710"
     CDS             complement(2555..2866)
                     /locus_tag="VHF88_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161302.1"
                     /translation="MSYPVDLALDQWRMGQSRVIGDPDLADASDAVLAELRKRLGGVF
                     QITELADLYGEGTDWASEVAQRYAQGTDSSWAIDAAFARYAQQAADFAGGRMQRFADE
                     D"
     gene            complement(2878..>3171)
                     /locus_tag="VHF88_05715"
     CDS             complement(2878..>3171)
                     /locus_tag="VHF88_05715"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX2161303.1"
                     /translation="GIRTSGIRWDRLSRVALLALLGAILLMYVSPAKHWWESSRTASA
                     QSEELSHLEAENKRLKQRAALLKNPDTLELEARKLGMTRSDERAFVIENPPRR"
BASE COUNT          504 a         1122 c         1106 g          439 t
ORIGIN      
        1 gacgtcgtac cagaggtagg tgtagtcggg gatcgccttg ctctggacca tgaagtcgat
       61 ccgctgctcc cagtcggccc agtcgtcgta gcgcggcggg attccgacgc gcgggaaggc
      121 gcggaagatc ggcgtccgcg acgacagcag gccggtcgcg tcggcccgcc agaagggcga
      181 gttcgccgac agcgcgagca ggatcggcag gtggacgcgc atcccgttgg cgatgtggat
      241 cgccttgtcg gggtcgtcga cgccgacgtg gacgtggatg ccgaagatca gctcctggcg
      301 cgcgacccac tgcagaccgg cgaccagctc gcggtagcgc ggccgcgaga cgatccgctg
      361 gtcctcccac atcgcgaacg gatgggtccc ggcggctccg atcgccagcc cccgctcggc
      421 ggctgcgccc atcacgagtc ggcgcagcgc gtcgagctgg cgaccggcgg cggccgtgtc
      481 ggcgcacggg gtcgtcgcga cctcgcagac cgactccatc agctccggct tgacctcgcc
      541 ctcggcgtcg tggccctcga ggtccgtcag cagcccctcg atcgagttcg ccagatcgag
      601 cgtctccgca tcgacgatca tcagctcctc ctcgacaccg acggtgtagg agggcccggt
      661 gaaccggtgc tccacgagcg aagggtatcc gccccggcgg ccccgcaaac cccgctgggc
      721 aggggtaacc tgggggtttc acgcaatcct gaggaggcaa caccccgaat gagcctcggc
      781 tacgacggca agctctacat cctcgcgttc gaccaccgcg ggtccttcca gaagaagatg
      841 ttcggcatcg agggcgaccc cacgcccgag cagacggcga cgatcgccga cgcgaagcac
      901 ctgatcttcg aggggatggc gaaggccgcc gaccgcggcg ccgaccccca cgcctccggc
      961 gcgctcgtcg acgagcagtt cggcggcgac atcccgcgcg acgcaaaggc gcgcggcctg
     1021 aagctggcga tgccggtcga ggcaagcggt cgcaatgact tcgagttcga gtacggcgac
     1081 gcgttcggcg agcacatcct gaagttcgac cccgacttct cgaaggtcct cgtgcggctc
     1141 aaccccgacg gcgacgccga gatgaacgag acccagctcg gcaagctcaa gcgcctctcg
     1201 gactggctca agcagaacga ccgcaagttc ctcttcgagc tgctcgtgcc ggccgagccg
     1261 gcccagctcg agtcggtcgg cggcgacacc gaccgctacg acgccgagct gcggccggac
     1321 ctgatgcgcc gggcgatcga gcagatccag gacgccggca tcgacgtcga catctggaag
     1381 atcgagggcg tcgacgagca ggccgactgc gagatgctcg ccgaggtgac ccgccgcgac
     1441 ggccgcgacg gcgtcgtctg cgtgctgctc ggccgcggcg cgagcgacgc gaaggtcgac
     1501 cactggctgc gccaggcggc tccggtcgac ggcttcgtcg gcttcgcgat cgggcgctcg
     1561 atctggtggg acgccctcaa gggcttcctc gacgggtcga tcgctcgcga ggacgcctcg
     1621 gaccagatcg cgtcgaacta cctgcgcttc gtcgaggtct acgagggcgc ggagcagggc
     1681 gcacacgtct agggttcctg cgatgcgcat gcgccagtcg cttcatcagt tcgaggcggc
     1741 cttccgcgag gaggccgccg attcggtcgc gcgccgggag cgcctgcgcc gcgaggcggc
     1801 gcagcgttcg cgggcgcgcc gcgtcgagcg ggtgcacaag cacggcacgc tgcgcttcat
     1861 cggactgatc gcgacgatcc tgctcacgac ggcgatcgtc acctgggcga tgttccaggt
     1921 gctggcctcc gtagccggct gaaccgctag tctccccgcc gccttggcac gccgagcact
     1981 caaaccgcag ctgaaccaga tccgcacgtg ggtgcggcag gggcgcaccg acgcctggat
     2041 cgcccaccag ctcgaggtgt ccgtcaagga cgtgacgaac ttcaagtcgg agcacgacct
     2101 cgaggccggc gagaccgagg gcgtcgagac cgacctcttc ggcccgcgcg tcgacgagga
     2161 ggagatcgac ctgcgcgccg aggacgaggc actggtcgcc gccgcgctcg aggaggaggc
     2221 ctcgcgccag tccgagcagg aggacgagga ggagcccacc gacgaggagg gcgaggcgcg
     2281 cgagggcgag gagagcgagg agagcaggtc acgccgccgg cgcgggcgcc gcggcggccg
     2341 cgggcgcaag cgcggcggtc gcaagaaggc gctcgagggc accttcgacc acggcgagga
     2401 cggctacggc ctctggctcg accccgcgat ccaggacgac ccgctctacg ccgagcactg
     2461 ggccggtcac cgcccggtca ccgtcacggt cgaggccgag cggatcgtga ttgagcgcct
     2521 caccgtctcc tccgacggcg aatagccctc ccggctagtc ctcgtctgcg aagcgctgca
     2581 tccggccccc ggcgaagtcg gcggcctgct gcgcgtagcg ggcgaacgca gcgtcgatcg
     2641 cccagctcga gtcggttccc tgcgcgtacc gctgcgcgac ctcggaggcc cagtcggtcc
     2701 cctcgccgta cagatcggcc agctcggtga tctgaaagac gccgccgagc cgcttgcgca
     2761 gctccgccag caccgcgtcc gaggcgtccg ccaggtccgg gtcgccgatc acccggctct
     2821 gccccatgcg ccactgatcg agcgccagat cgactggata cgacacagcc ggcgaggtta
     2881 gcgacgcggc gggttctcta ttacgaacgc tcgctcgtcg ctgcgcgtca tgcccagctt
     2941 gcgcgcctcg agctcaagcg tgtccgggtt cttgagcagc gccgcgcgct gcttgagccg
     3001 cttgttctcg gcctcgaggt gggacagctc ctcgctctga gccgaggccg tccgcgagga
     3061 ctcccaccag tgcttggcgg gagacacgta catcagcagg atcgcgccga gcagtgccag
     3121 cagcgcgacc cggctgagcc ggtcccagcg gatcccgctc gtccttattc c
//
LOCUS       DATABV010000133         5815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2291432, whole genome shotgun sequence.
ACCESSION   DATABV010000133 DATABV010000000
VERSION     DATABV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291432"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VHF88_05720"
     CDS             complement(<1..728)
                     /locus_tag="VHF88_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2161304.1"
                     /translation="MATTITVLSQKGGTGKTTTTRTLVDVFRRIDLRVLAVDMDPQGN
                     LTDYFAVGDEPEPTLADVLAGKAEAAAAIHSDVLPASVHLAEAELLLSGKIGREMVLR
                     RALATVGDDYDLILIDCPPSLGLLTVNALVAADHALISTEAEYFSLQGVEQALEVVEA
                     ARGVSPDLQWLGAVLNIADMRTVHAREALEQLRDGYGDKIFDTPIRRSVAYAESAERA
                     ISIVDHRPDLGADYLSLADEVLQRL"
     gene            891..5426
                     /gene="gltB"
                     /locus_tag="VHF88_05725"
     CDS             891..5426
                     /gene="gltB"
                     /locus_tag="VHF88_05725"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX2161305.1"
                     /translation="MSHPVYPPGGRGIAPSSASGLHDHRYEHDACGVGFVARLHAPAS
                     HEVVERALEVLNRMEHRGAEGADPETGDGAGILIQVPDAFLRAEVDFELPPPGRYGVA
                     MCFLPPRAEDRPAAVRALEQAVEAQGLWVLGWRDVPVDPDACGRTAAKCAPYTAQLFI
                     GAPDGVADQDALERRLYVARRAAEDAEVLGFSITSCSSRTVVYKGMLIAPQLPRYFPD
                     LQDERVASRLALVHSRFSTNTFPSWELAHPYRMLAHNGEINTVRGNRNWMRARESQLS
                     SELFGDALSDLGPLLDDRMSDSASLDAAFELLTLGGRSMAHAMSMLIPEAYQGRGELP
                     PEVNDFFAYHDSLIEPWDGPAAVAFTDGRAIGATLDRNGLRPGRWLHTRDGWVVFASE
                     TGVLPVPEEDVVGKGRLHPGKLFLVDLEGERVVPDAELKSDLAARQPYGEWLSERVVR
                     IGDLPDKSPRVPRVEPLRARQLAFGWSQEDLRVLLEPMARDGMEPTGSMGNDTALAVL
                     SDNRPPLFSYFKQLFAQVTNPAIDPIRESIVMSLQACVGPEINLLGETPDHCHQLVMP
                     QPILRNSELEKLRQVDHAVFQARSIDITWPVRDGVSGLERRLEEICAQAAELVEDGVT
                     ILILSDRNLGRERAAIPSLLATAAVHHHLVRAATRLRCGLVVETGEAREVHHIACLTG
                     YGAAAVNPYLLFESLGQVHHTGRLPEDMTLEQAEANVVKAIGKGLLKVLSKMGISTMR
                     SYTGAQIFEAIGLDEEMVDRHFSGTPSRIGGVGFDVLAGEALDRHRRAYPAAASDLLP
                     SGGVYAWRRDGEFHGWNPETIANLQHAARGEGGPESYERFKTYVNEVAAKNSTIRGLL
                     DFNDDVEPVPLDEVEPASEIVKRFKSGGMSLGALSPEAHEGLAVAMNRLGGKSNTGEG
                     GEDRARFGDERRSSIKQVASGRFGVTIEYLVNADEIQIKVAQGAKPGEGGQLPGHKVD
                     SYIAGLRHSTPGVTLISPPPHHDIYSIEDLKQLIYDLRCANPKARVSVKLVSEVGVGT
                     VAAGVAKANADHVVIAGHDGGTGASPQSSIQHAGIPWEIGLAETQQTLLRNDLRSRIV
                     VETDGQMRTGRDVMVAALLGADEYGFSTAPLIALGCVMMRVCHLNTCPVGVATQDPAL
                     RARFAGEPDHVVNYLVMVAEEVRELLASLGLRSLDEAIGRTDLLVPDDKVDHWKARGV
                     DLRALLVVPEEAEDGRPRHNTDPPPPVLDDALDHDLIAACAPAIERGEQVRLERTIRN
                     ENRTVGGLLSNAIARARGANGLPDGTVEIALRGSAGQSFGAWLAPGVTLTLDGDANDY
                     VGKGLSGGTVVVRPACEASFTAEANVIVGNTVLYGATAGKAFFRGLAGERFAVRNSGA
                     SAVVEGVGDHGCEYMTGGRVVVIGPTGLNFAAGMSGGVAYVLDADGGFAGRCNMELVD
                     LEAPAPDDLEEVRALLAEHAERTGSPVAHGLLEGWDGAAGRFVKVMPRDYKRALAELA
                     KAETDAATAESKLGAPA"
     gene            5426..>5815
                     /locus_tag="VHF88_05730"
     CDS             5426..>5815
                     /locus_tag="VHF88_05730"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161306.1"
                     /translation="MGEQGAFLRIHRVGFDKRDPQERVNDYKQYFELPDEQTLREQGA
                     RCMDCGIPFCHEGCPLGNPIPEWNDLVYQGRWRDAVKIAHSTNNFPEFTGLICPAPCE
                     SACVLNINDDPVAIEEIELAIATRGFDE"
BASE COUNT          850 a         2130 c         2037 g          798 t
ORIGIN      
        1 agccgctgca gcacctcgtc cgccagagac aggtagtcgg ccccgaggtc ggggcggtgg
       61 tcgacgatcg agatcgcgcg ctcggccgac tcggcgtagg cgaccgaccg ccggatcggg
      121 gtgtcgaaga tcttgtcgcc gtagccgtcg cgcagctgct cgagcgcctc gcgggcgtgg
      181 acggtgcgca tgtcggcgat gttcaggacc gctccgagcc actgcaggtc cgggctcacg
      241 ccacgcgccg cctcgacgac ctcgagcgcc tgctcgacgc cctgcagcga gaagtactcg
      301 gcctcggtcg agatcagcgc gtggtcggcc gccaccagcg cgttgaccgt cagcagcccg
      361 agcgatggcg ggcagtcgat caggatcagg tcgtagtcgt cgccgaccgt cgcgagcgca
      421 cgccgcagca ccatctcgcg gccgatcttg ccgctcagca gcagctccgc ctcggcgagg
      481 tggacgctcg ccggcagcac gtcggagtgg atcgccgcgg cggcctccgc cttgccggcc
      541 agcacgtcgg ccagcgtcgg ctcgggctcg tcgccaaccg cgaagtagtc ggtcaggttg
      601 ccttgcgggt ccatgtcgac cgcgagcacg cgcaggtcga tgcgccggaa gacgtcgacg
      661 agcgtgcgcg tcgtcgtcgt cttcccggtg ccccccttct gcgacaggac ggtgatcgtg
      721 gtcgccatcg cgccgacgat acagacggca tgcgtcgccg cgtgcgccgg tcgacacagg
      781 tcgcacaggc gaaccgcttc cccctcccga cagccccgca acgctaagat ttcgcctcct
      841 gactcacgcg tgcctgctgg gagatacgcg cgaacccgga ggaaggacag atgtcgcatc
      901 ccgtctaccc gccgggcggc aggggaatcg ctccctcgtc agccagtggg ctacatgacc
      961 atcggtacga gcacgacgcc tgcggcgtcg gcttcgtcgc gcgcctgcac gcgccggcct
     1021 cgcacgaggt cgtcgagcgg gcgctcgagg tcctcaaccg gatggagcat cgcggggccg
     1081 agggggccga ccccgagacg ggcgacggcg ccggcatcct gatccaggtc cccgacgcgt
     1141 tcctgcgcgc cgaggtcgac ttcgagctgc cgccccctgg gcgctacggc gtggcgatgt
     1201 gcttcctgcc ccctcgcgcc gaggaccgcc cggcggccgt gcgggcgctc gagcaggcgg
     1261 tcgaggcgca gggcctctgg gtgctcggct ggcgcgacgt gccggtcgat cccgacgcct
     1321 gcggccggac cgcggcgaag tgcgcgccgt acaccgccca gctgttcatc ggcgcgcccg
     1381 acggcgtcgc cgaccaggac gcgctcgagc gcaggctcta cgtcgcgcgc cgcgccgccg
     1441 aggacgccga ggtgctcggc ttctcgatca ccagctgctc gtcgcgaacc gtggtctaca
     1501 aggggatgct gatcgcgccg cagctgccgc gctacttccc ggacctccag gacgagcgcg
     1561 tcgccagccg cctggcgctc gtccactcgc gcttctcgac caacaccttc ccgagctggg
     1621 agctggcgca cccgtaccgc atgctcgctc acaacggcga gatcaacacc gtgcgcggca
     1681 accgcaactg gatgcgcgcg cgcgagtcgc agctgtcttc ggagctgttc ggcgacgccc
     1741 tgtccgacct cggcccgctg ctcgacgacc ggatgtcgga ctcggcctcg ctcgacgcgg
     1801 ccttcgagct gttgacgctc ggcgggcgct cgatggccca cgcgatgtcg atgctgatcc
     1861 ctgaggccta ccagggccgc ggcgagttgc cgcccgaggt caacgacttc ttcgcctacc
     1921 acgactcgct gatcgagccg tgggacggcc cggcggcggt cgcgttcacc gacggccgcg
     1981 cgatcggcgc gacgctcgac cgcaacgggc tgcgtccggg ccgctggctg cacacccgcg
     2041 acggctgggt cgtcttcgcc tccgagacgg gggtgctgcc ggtgccggag gaggatgtcg
     2101 tcggcaaggg ccgcctacac cccggcaagc tgttcctggt cgacctcgag ggcgagcgcg
     2161 tcgtgcccga cgccgagctc aagagcgacc tcgcggcgcg ccagccctac ggcgagtggc
     2221 tctccgagcg cgtcgtgcgg atcggcgacc tgccagacaa gagcccgcgc gtcccgcgcg
     2281 tcgagccgct gcgcgcgcgc cagctcgcgt tcggctggag ccaggaggac ctgcgtgtgc
     2341 tgctcgagcc gatggcgcgc gacggcatgg agccgaccgg ctcgatgggc aacgacaccg
     2401 cgctcgcggt cctctcagac aaccgcccgc cgctgttctc ctacttcaag cagctgttcg
     2461 cccaggtcac caacccggcg atcgacccga tccgcgagtc gatcgtgatg agcctgcagg
     2521 cctgcgtcgg ccccgagatc aacctgctcg gcgagacgcc cgaccactgc caccagctcg
     2581 tgatgccgca gccgatcctg cgcaactccg agctcgagaa gctgcgtcag gtcgaccacg
     2641 ccgtcttcca ggcgcgctcg atcgacatca cctggccggt ccgcgacggc gtctcgggac
     2701 tcgagcggcg gctcgaggag atctgcgccc aggcggccga gctggtcgag gacggcgtga
     2761 cgatcctgat cctctccgac cgcaacctcg gccgcgagcg cgcggcgatt ccgtcgctgc
     2821 tggcaaccgc ggccgtccac caccaccttg tgcgcgcggc gacgcggctg cgctgcggcc
     2881 tcgtcgtcga gaccggcgag gcgcgcgagg tccaccacat cgcatgcctg accggctacg
     2941 gcgcggcggc ggtcaatccc tacctgctgt tcgagtcgct cggccaggtc caccacaccg
     3001 gccgcctgcc ggaggacatg acgctcgagc aggccgaggc gaacgtcgtc aaggcgatcg
     3061 gcaagggtct gctgaaggtg ctctcgaaga tgggcatctc gacgatgcgc tcctacaccg
     3121 gcgcgcagat cttcgaggcg atcgggctcg acgaggagat ggtcgaccgt cacttcagcg
     3181 gcacgccgtc gcggatcggc ggcgtcggct tcgacgtgct tgccggcgag gcgctcgacc
     3241 gccaccggcg cgcctacccc gccgcggcat cggacctgct gccgtccggg ggcgtctacg
     3301 cctggcgccg cgacggcgag ttccacggtt ggaaccccga gacgatcgcc aacctccagc
     3361 acgctgcgcg cggcgagggc ggcccagagt cctacgagcg cttcaagacc tacgtcaacg
     3421 aggtcgcggc caagaactcg acgatccgcg gcctgctcga cttcaacgac gacgtcgagc
     3481 cggtgccgct cgacgaggtc gagccggcga gcgagatcgt caagcggttc aagagcggtg
     3541 ggatgagcct cggcgcgctc tcgcccgagg cccacgaggg actcgcggtc gctatgaacc
     3601 ggctcggcgg caagtcgaac accggcgagg gcggcgagga ccgcgcccgc ttcggcgacg
     3661 agcggcgctc gtcgatcaag caggtcgcct ccggccgctt cggcgtgacg atcgagtacc
     3721 tcgtcaacgc cgacgagatc cagatcaagg tcgcccaggg cgccaagccc ggcgagggcg
     3781 ggcagctgcc cggccacaag gtcgactcct acatcgccgg actgcgccac tcgacgccgg
     3841 gcgtgacgct gatctcgccg ccgccgcacc acgacatcta ctcgatcgag gacctcaagc
     3901 agctgatcta cgacctgcgc tgcgcgaacc cgaaggcgcg cgtctcggtc aagctggtct
     3961 cggaggtcgg cgtcggcacg gtcgccgccg gcgtcgcgaa ggcgaacgcc gaccacgtcg
     4021 tgatcgccgg gcacgacggc gggaccggcg cgtcgccgca gtcgtcgatc cagcacgccg
     4081 gcatcccgtg ggagatcggg ctggccgaga cgcagcagac gctgctgcgc aacgacctgc
     4141 gctcgcggat cgtcgtcgag accgacggcc agatgcggac cggccgcgac gtgatggtcg
     4201 cggcgctgct cggcgccgac gagtacggct tctcgacggc tccgctgatc gcgctcggct
     4261 gcgtgatgat gcgcgtctgc cacctcaaca cctgtccggt cggggtcgcg acccaggacc
     4321 ccgcgctgcg cgcgcgcttc gccggcgagc ccgaccacgt cgtcaactac ctcgtgatgg
     4381 tcgccgagga ggtgcgcgag ctgctcgcgt cgctcggcct gcgcagcctc gacgaggcga
     4441 tcggccgcac cgacctgctc gtgcccgacg acaaggtcga ccactggaag gcccgtggag
     4501 tcgatctgcg cgcgctgctc gtggtgccgg aagaggccga ggacgggcgt ccccgtcaca
     4561 acaccgatcc gccgccgccg gtgctcgacg acgcgctcga ccacgacctg atcgccgcct
     4621 gcgcgcccgc gatcgagcgc ggcgagcagg tccggctcga gcgcacgatc cgcaacgaga
     4681 accgcacagt cggcgggctg ctttcgaacg cgatcgcgcg ggcgcgcggc gcgaacgggt
     4741 tgcccgacgg cacggtcgag atcgcgctgc gcggctcggc cggccagagc ttcggcgcct
     4801 ggctggcgcc cggcgtcacg ctgacgctcg acggcgacgc caacgactac gtcggcaagg
     4861 gcctgtcggg cgggacggtc gtcgtccgcc cggcctgcga ggcgagcttc acggccgagg
     4921 cgaacgtgat cgtcggcaac accgtcctct acggcgcgac cgccgggaag gccttcttcc
     4981 gcggcctggc cggagagcgc ttcgcggtcc gcaactccgg tgccagcgct gtcgtcgagg
     5041 gcgtcggcga ccacggctgc gagtacatga ccggtggccg cgtcgtcgtg atcggcccga
     5101 ccgggctcaa cttcgccgcc ggcatgagcg gcggcgtcgc ctacgtgctc gacgccgacg
     5161 gcggcttcgc cgggcgctgc aacatggagc tcgtcgacct cgaggcgccg gcgcccgacg
     5221 acctcgagga agtgcgcgcg ctgctggccg agcacgccga gcggacgggg tcgccggtcg
     5281 cccacgggct gctcgagggc tgggacggcg ccgccgggcg cttcgtcaag gtgatgccgc
     5341 gcgactacaa gcgcgcgctc gccgagctgg cgaaggccga gaccgacgcc gccaccgccg
     5401 agagcaagct gggggcgcct gcctgatggg cgagcaggga gcgttcttgc gcatccaccg
     5461 ggtcggcttc gacaagcgcg atccgcagga gcgcgtcaac gactacaagc agtacttcga
     5521 gctgcccgac gagcagacgc tgcgcgagca gggcgcccgc tgcatggact gcggcatccc
     5581 gttctgccac gagggctgcc cgctcggcaa cccgatcccc gagtggaacg acctcgtgta
     5641 ccagggccgc tggcgcgacg cggtcaagat cgcccactcg acgaacaact tccccgagtt
     5701 caccggcctg atctgcccgg cgccgtgcga gtcggcctgc gtgctgaaca tcaacgacga
     5761 cccggtcgcg atcgaggaga tcgagctggc gatcgcgacg cgcggcttcg acgag
//
LOCUS       DATABV010000134         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_122290, whole genome shotgun sequence.
ACCESSION   DATABV010000134 DATABV010000000
VERSION     DATABV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122290"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VHF88_05735"
     CDS             <1..270
                     /locus_tag="VHF88_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2161307.1"
                     /translation="LELRTLRRRLGWSKANATEVTATLEARGLVLRHRLERDRRAVAL
                     DLTPAGRSVVERLFPAHADRVTSAFSRLDDDEKRNLADICRKLAA"
     gene            complement(357..1094)
                     /locus_tag="VHF88_05740"
     CDS             complement(357..1094)
                     /locus_tag="VHF88_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161308.1"
                     /translation="MGEAEMRKFSIAGAAVLGVLASVAIGAIAQDDVQTTFTIDAAVL
                     PNKAGTPKDPQGVKIKASVRFFTPQGVDPPIVTHGYALFPRNGDYNPHLYPRCDKRTL
                     DRDGPSRCPKGSQIGAARASAYADTIITRPRIEIFNGGPKVAFAYVTLYRPALVQTAV
                     PARIREIPRGKWKYKVSVRVPEVLQVVAGIPIAARSFKGYVGKGNLIVTTSCPKSRRW
                     PYEVKTYFTIGTPYKYRDSVPCRPAGR"
     gene            1154..>3482
                     /gene="ileS"
                     /locus_tag="VHF88_05745"
     CDS             1154..>3482
                     /gene="ileS"
                     /locus_tag="VHF88_05745"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX2161309.1"
                     /translation="MPRYAPVDSRQSFPALEEEMLARWRERDVFAESVRRREGAEPFV
                     FYEGPPTANGRPGSHHVLSRVFKDVFPRYQTMRGRQVRRKAGWDCHGLPVELEIEKEL
                     GLAHKRDIEDYGIDKFNAKCRESVLRYIDEWNRLTERIGFWIDTDDAYYTLDNDYIES
                     VWWSLKQVWDKGLLYEGHKVGPYCPRCGTGLSSHEVALGYKDVVDPSIYVRFPLVSND
                     GATTGESLLVWTTTPWTLPGNVAVAVAPKVTYVRARVGDETLILAEPLVGRVLGDDAQ
                     ILESFPGADLIGRAYRGPIFQLADGGPQEAFRVIAGEFVTTDDGTGLVHLAPAFGEDD
                     FDAAAAAGIFDPAAAAPSTVDGVHAAATEAASGPALYNPVRRDGTFDDRVAGFAGRFV
                     KDPDVTAALIADLDARGALFKAEDYDHSYPHCWRCDTPLLYYAKSSWYVRTTAVKDEL
                     LAANERVTWYPEHIKHGRFGNWLENNVDWALSRERYWGTPLPIWRSEDGEETICIGSR
                     AELERLAGDLPDDLHRPYVDDVVFEHRGRVFRRVPDLIDVWWDSGAMPFAQWHQPFEN
                     ADEFEQSFPADYICEALDQTRGWFYSLLAISTLLFDRSSYETVLCLGLILDEQGQKMS
                     KSKGNVVVPWDVLDQHGADAFRWYYFTSKQPWDGYRFSLDTVGESVRQFMKPLWNTYS
                     LFVLYANVNDVEPVPLSELAAVAGAAGPDAQPPLTDLDRWILSRLQATTATAIDRLDD
                     FDTTAAGRAVAAFVDELSNWYVRRSRRRFWDGDPAA"
BASE COUNT          525 a         1294 c         1141 g          522 t
ORIGIN      
        1 ctcgagctgc gaacgctgcg ccgcaggttg ggctggtcga aggcgaacgc caccgaggtc
       61 accgccacgc tcgaggcgcg cggcctggtc ctgcgccacc gcctcgagcg cgaccgccgc
      121 gcggtggcgc tcgacctcac ccccgccggc cgctcggtcg tcgagcgcct cttccccgca
      181 cacgccgacc gcgtcacctc cgccttctcc cgcctcgacg acgacgagaa gcgcaacctc
      241 gcggacatct gccgcaagct cgccgcctga gcgcgtcttg cgagacgctc ccgcggaggc
      301 tcccgtcccg gcatcgacga ccggcggcgg gcgcgcgaca gcggccagcg cttcgactac
      361 cggccagccg gccggcacgg caccgagtcg cggtacttgt agggcgtgcc gatcgtgaag
      421 taggtcttga cctcataggg ccagcggcgt gacttcggac acgacgtcgt gacaatcagg
      481 ttgcccttgc cgacgtagcc cttgaaggac cgggcggcga tcgggatccc ggctaccacc
      541 tggagcacct ccggcacccg caccgagacc ttgtacttcc acttcccgcg cgggatctcc
      601 cggatgcgcg ccgggaccgc ggtctgaacg agcgccggcc ggtacagcgt cacataggcg
      661 aaggcgacct tcgggccacc gttgaagatc tcgatccgcg gccgggtgat gatcgtgtcg
      721 gcgtaggccg acgcgcgcgc cgccccgatc tgcgagccct tcggacagcg gctcggcccg
      781 tcccgatcga gcgtccgctt gtcgcagcgc ggatacagat gcgggttgta gtcgccgttg
      841 cgcgggaaca gcgcatagcc gtgggtgacg atcggcgggt cgacgccctg cggggtgaag
      901 aagcgcaccg acgccttgat cttcacgccc tgagggtcct tcggcgtccc agccttgttc
      961 gggagcaccg cggcgtcgat cgtgaacgtc gtctggacgt cgtcctgcgc gatcgcgccg
     1021 atggcgaccg aggcgaggac gccgagcaca gccgctcctg caattgaaaa cttgcgcatc
     1081 tccgcctctc ccacccgacc atatacccgg cgccccgggc cgcccaaggg ccaaccgtag
     1141 aatccccagc ccgatgcccc gctacgcgcc cgtcgactcg cgtcagtcgt ttcccgccct
     1201 cgaggaggag atgctggcgc gctggcgcga gcgagacgtg ttcgccgagt cggtccgccg
     1261 ccgcgagggc gccgaaccgt tcgtcttcta cgagggcccg ccgaccgcca acgggcgccc
     1321 gggctcgcac cacgtcctct cgcgcgtctt caaggacgtc tttccccgct accagacgat
     1381 gcgcggccgc caggtgcggc gcaaggccgg ctgggactgc cacggcctgc cggtcgagct
     1441 cgagatcgag aaggagctcg gactcgcgca caaacgtgac atcgaggact acggaatcga
     1501 caagttcaac gcgaagtgtc gcgagtcggt gctgcgctac atcgacgagt ggaaccgcct
     1561 gacggagcgg atcgggttct ggatcgacac cgacgacgcc tactacacgc tcgacaacga
     1621 ctacatcgag tcggtctggt ggtcgctgaa gcaggtctgg gacaaggggc tgctctacga
     1681 gggccacaag gtcggcccgt actgcccgcg ctgcggtacc gggctttcga gccacgaggt
     1741 cgcactcggt tacaaggacg tcgtcgaccc gtcgatctac gtgcgcttcc cgctcgtcag
     1801 taacgatggc gcaacgaccg gcgagtcgct gctcgtctgg acgaccactc cgtggaccct
     1861 gcccgggaac gtcgcggtcg ccgtcgcgcc gaaggtcact tacgtgcgcg cgcgggtcgg
     1921 cgacgagacg ctgatcctgg ccgagccgct cgtcggtcgc gtgctcggcg acgacgcgca
     1981 gatcctcgag agcttccccg gcgccgacct gatcggccgc gcctaccgcg gcccgatctt
     2041 ccagctcgcc gacggtggac cgcaggaggc gttccgggtg atcgccggcg agttcgtcac
     2101 caccgacgac ggcaccggcc tcgtccacct cgcgccagcg ttcggcgagg acgacttcga
     2161 cgcggcagcg gcggccggga tcttcgaccc cgccgccgcg gcgccgagca ctgtcgacgg
     2221 cgtccacgcc gccgccacgg aggccgccag cggccccgcg ctctacaacc cggtccgtcg
     2281 cgacggcacc ttcgacgacc gcgtcgccgg cttcgccggc cgcttcgtca aggaccccga
     2341 cgtcaccgct gcgctgatcg ccgacctcga cgcgcgcggc gcactcttca aggccgagga
     2401 ctacgaccac tcctaccccc actgctggcg ctgcgacacg ccgctgctct actacgccaa
     2461 gtcgagctgg tacgtgcgca cgacggcggt caaggacgag ctgctggcgg ccaacgagcg
     2521 cgtcacctgg taccccgagc acatcaagca cgggcgcttc ggcaactggc tcgagaacaa
     2581 cgtcgactgg gcgctgtcgc gcgagcgtta ctggggcacg ccgctgccga tctggcgctc
     2641 cgaggacggc gaggagacga tctgcatcgg ctcgcgcgcc gagctggagc ggctggccgg
     2701 cgatctgccc gacgacctcc accgccccta cgtcgacgac gtcgtcttcg agcaccgcgg
     2761 ccgggtcttc cgccgcgtgc cggacctgat cgacgtctgg tgggactccg gcgcgatgcc
     2821 gttcgcccag tggcaccagc cgttcgagaa cgccgacgag ttcgagcagt cgttccccgc
     2881 cgactacatc tgcgaggcgc tcgaccagac gcgcggctgg ttctactcgc tgctcgcgat
     2941 ctcgacgctg ctgttcgacc gctcctccta cgagaccgtg ctctgcctcg ggctgatcct
     3001 cgacgagcag ggccagaaga tgtcgaagtc gaagggcaac gtcgtcgtgc cgtgggacgt
     3061 cctcgaccag cacggcgccg acgccttccg ctggtactac ttcacctcga agcagccgtg
     3121 ggacggctac cgcttctcgc tcgacacggt cggcgagtcc gtgcggcagt tcatgaagcc
     3181 gctctggaac acgtactcgc tgttcgtgct gtacgcgaac gtcaacgacg tcgagccggt
     3241 gccgctgtcc gagcttgcgg cggtggccgg cgccgccggt cccgacgccc agcccccgct
     3301 gaccgacctc gaccgctgga tcctgtcgcg cctccaagcg acgaccgcga ccgcgatcga
     3361 ccgcctcgac gacttcgaca ccaccgccgc cggccgggcg gtcgccgcct tcgtcgacga
     3421 gctgtcgaac tggtacgtgc gccgctcgcg gcgccgcttc tgggacggcg acccagccgc
     3481 ct
//
LOCUS       DATABV010000135         6144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_41611, whole genome shotgun sequence.
ACCESSION   DATABV010000135 DATABV010000000
VERSION     DATABV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41611"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..921)
                     /locus_tag="VHF88_05750"
     CDS             complement(34..921)
                     /locus_tag="VHF88_05750"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX2161310.1"
                     /translation="MSRFEDPVVITCSISGAVANRDQCPAIPYTPEEYAAEARRIVDE
                     GGSMIHIHARTPDGVPSYEVEDFRAITAAIRAAVDDVIVNYSTGAIGVPIEKRIAYLR
                     ELRPDVAALNMSSMNYAKYSRSRKEFVFKAVFENSFDTIVEFLTAMNELGIRPEHECF
                     DTGHIANLDPLIDMGLLSEPLQISCVMGVTGGVRPTPRNLAHMADQVPGGPDGPNNWG
                     VIGVSRAQWRLVAAALALGGNVRVGLEDNFYLPDGEMARSNGDLVAKARQMTEDVGRH
                     PATVAEARELLGVPRRTTV"
     gene            complement(918..1886)
                     /locus_tag="VHF88_05755"
     CDS             complement(918..1886)
                     /locus_tag="VHF88_05755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX2161311.1"
                     /translation="MRAVQVSELSGPAAVRVVNVDEPVGDGQNLLVDVKAAGVSFPEL
                     LQTRGDYQLKLDPPFVLGSELSGVVREAPDGSGFAPGDRVTALAPLGGAFAEVALAPP
                     FAAFKLPDALDFGQGAGYCMNYHTAHFALARRAGLRAGETLLVHGAAGGTGTAAIQVG
                     KGLGARVVAVVSTDEKEQVARKAGADEVVRSDGDWRKDAGPANVIFDPVGGSRFDESV
                     RSLAPEGRLVVIGFTEGSIPSVSVNRLLFRNVSVVGAGWGHFAFERPQYLREVAADLD
                     RMVAAGNVDPIVGRRYPLEGVREALEDLDGRRAVGKLVLDVDQEAA"
     gene            complement(1883..3457)
                     /locus_tag="VHF88_05760"
     CDS             complement(1883..3457)
                     /locus_tag="VHF88_05760"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2161312.1"
                     /translation="MPTDAPPESLLRPLVEDLHARREKAKLGGGPEKIERQHAADKLT
                     ARERLDLLIDAGSFTELGIHGRPHFAQRAMEGKEAPADGVVTGYGKVDGRLVAVCAYD
                     FTVMAGSMGMTGELKVTRLRELALQKRIPFIWLLDSAGARIQEAAGSLFAGSGFLFRE
                     EVVMSGVIPQIAALMGPCAAGTAYIPGLADFVPMVKGRGSMALAGPHLVKAVTGEDVT
                     QEELGGSKIHTKVSGVGDLEVKSDEECIAAIKDYLSYFPQNCEQAPPIRPTADPVDRM
                     DEELLDVLPPSNRQPYDMYDVIRRIVDDGVWFDIKPRFARTIVTCLARMGGRPVGIVA
                     NQPKHLGGILENDSADKAARFVNLCDAYGIPLLFLQDVPGFMVGTKVEHAGIIRHGAK
                     MLYAVSNATVPKVTVVVRKAYGAGYYVMCGKAYEPDLIVAWPSAEISVMGAEGAVNII
                     GRSLIEAADDPEATRKQMEDAIRATIDPYIAAGNAMIDDVIDPRETRPVVIKALEMAA
                     TKKVERPWKKMGVMPV"
     gene            complement(3511..4365)
                     /locus_tag="VHF88_05765"
     CDS             complement(3511..4365)
                     /locus_tag="VHF88_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX2161313.1"
                     /translation="MERAVNVSLVALRCRTSDRTAAAVRGVDELAPLIGKRLGREPRQ
                     IGSPGEPREANWEDDVSDSRGCLLEAGGQVDDALAAGRLPVLLAGECSVALTTLPTVL
                     RHRPDTVVLWLDAHGDFNTPDTTVSGSLSGMALAGACGLWNAGLIADTVPEDRVVLAG
                     VRDLDGDERKALERSDVTVVGASPVETLVAVKNALDGAPVYIHLDLDVIDPAVFPARE
                     PAPEGLAPEKLFDLIEAVMADCELVGVEVTTFEAPEDEEDRQEAAAVVMRVLEPLLEA
                     IPERAASA"
     gene            complement(4350..5135)
                     /locus_tag="VHF88_05770"
     CDS             complement(4350..5135)
                     /locus_tag="VHF88_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2161314.1"
                     /translation="MAFEKIRYEVDEAKVATITLDDPETRNALGSDTLTELIAAFEQA
                     RADDDVRCVVLTSSHDKVFSAGGNLAGFAAEMPLVHKHFGTELFPRLFRTIGQLGKPT
                     ICAANGHVLAGSLGVALACDLIVASDKATFGTPEINVGVFPFMIMALIYRNVGRKKTN
                     ELLLLGERVSAKEALEIGLVNKVVPAKGLEYEVRLWAEQLAKKSPLMMRLGKDAMYRQ
                     QDMAFDDALEYLHHNLTLSFSTDDIQEGVKAFFEKREPQWSGR"
     gene            complement(5146..5400)
                     /locus_tag="VHF88_05775"
     CDS             complement(5146..5400)
                     /locus_tag="VHF88_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX2161315.1"
                     /translation="MLERVRAIPPGRVTTYGDLSPGAPRFAGSVLSACDDPDLPWQRV
                     VRADGSLPKGDRQRGLLEAEGVPFRGARVDMGAAWMPVPG"
     gene            complement(5424..>6144)
                     /locus_tag="VHF88_05780"
     CDS             complement(5424..>6144)
                     /locus_tag="VHF88_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2161316.1"
                     /translation="DSLRAQGVDVRLEATVAAVRAETGAVAVELEGDPPVIADELLVA
                     AGRRPRTDDINLESVGLSPGESIDVDDQMRATGIDGDWLYAVGDVNGRALLTHMGKYQ
                     ARAAADVILGKHAAVTEDGPVSPRVVFTDPQVAAVGHTLASAQEAGLNVRAVDVGTEA
                     NAGGSFVGRGTAGTARIVVDEDRRVIVGATFTGAEVAESLHAATIAVVGELPLDRLWH
                     AVPAFPTRSELWLNLLEEYGL"
BASE COUNT          874 a         2194 c         2147 g          929 t
ORIGIN      
        1 ggtcgaggac ccggatgtcg gaaagcgcac ccactacacc gtcgtcctgc gcggcacgcc
       61 gagcagctcg cgggcctcgg cgacggtggc ggggtggcgg ccgacgtcct cggtcatctg
      121 gcgcgctttg gcgaccaggt cgccgttgct gcgcgccatc tcgccgtcgg gcaggtagaa
      181 gttgtcctcg aggccgacgc ggacgttgcc gccgagcgcc agcgcggcgg cgacgagccg
      241 ccactgggcg cggctgacgc cgatcacgcc ccagttgttg ggcccgtcag gaccgcccgg
      301 cacctggtcg gccatgtgcg cgaggttgcg cggggtcggc cgcacgccgc cggtcacccc
      361 catcacgcac gagatctgca gcggctcgga cagcagcccc atgtcgatca gcggatcgag
      421 gttggcgatg tggccggtgt cgaagcactc gtgctcgggc cggatcccga gctcgttcat
      481 cgccgtcagg aactcgacga tcgtgtcgaa cgagttctcg aagacggcct tgaagacgaa
      541 ctccttgcgc gagcgcgagt acttcgcgta gttcatcgag ctcatgttga gcgcggcgac
      601 gtcaggccgc agctcgcgca ggtacgcgat ccgcttctcg atcggcacgc cgatcgcgcc
      661 ggtcgagtag ttgacgatca cgtcgtcgac cgccgcccgg atcgccgcgg tgatcgcgcg
      721 gaagtcctcg acctcgtagc tcggcacgcc atcgggcgtg cgggcgtgga tgtggatcat
      781 cgagccgccc tcgtcgacga tccgacgcgc ctcggcggcg tactcctcgg gcgtgtaggg
      841 gatcgccggg cactggtcgc ggttggcgac ggccccggag atggagcagg tgatcacgac
      901 cgggtcctcg aagcggctca tgcggcctcc tgatcgacgt cgagcacgag cttgccgacg
      961 gcccgccggc cgtcgaggtc ctcgagcgcc tcgcggacgc cctcgagcgg gtagcggcga
     1021 ccgacgatcg ggtcgacgtt gccggcggcg accatccggt cgagatcggc ggcgacctcg
     1081 cgcaggtact gcggccgctc gaaggcgaag tgcccccacc ccgctccaac cacgctgacg
     1141 ttgcggaaca gcagccggtt gacggacacg ctcgggatcg agccctcggt gaagccgatc
     1201 acgacgagtc ggccctccgg cgccagcgag cggacactct cgtcgaagcg cgagccgccg
     1261 accgggtcga agatcacgtt cgccgggccg gcgtcctttc gccagtcgcc gtcgctgcgc
     1321 acgacctcgt cggcgccggc cttgcgcgcc acctgctcct tctcgtcggt cgacacgacg
     1381 gccacgacgc gcgctccgag ccccttgccg acctggatcg ccgccgtccc ggtcccaccg
     1441 gcggcaccgt ggacgagcag cgtctccccc gcgcgcagcc ccgcccgacg tgccagcgcg
     1501 aagtgggcgg tgtggtagtt catgcagtag ccggcgccct ggccgaagtc gagcgcgtcg
     1561 gggagcttga aagcggcgaa gggcggcgcc agcgcgacct ccgcgaacgc cccgccgagc
     1621 ggcgcgagcg ccgtgacccg atctccgggc gcgaagccgg agccgtcggg cgcctcgcga
     1681 acgaccccgg acagctcgct tccgaggaca aacggcgggt cgagcttgag ctggtagtcg
     1741 ccgcgggtct gcagcagctc gggaaacgac acgcccgccg ccttgacgtc gacgagcagg
     1801 ttctggccgt caccgaccgg ctcgtcgacg ttgaccaccc gcaccgccgc cggaccgctc
     1861 agctcgctga cctggacggc cctcataccg gcatcacgcc catcttcttc cacggccgct
     1921 cgaccttctt ggtcgccgcc atctcgagcg ccttgatcac caccggccgt gtctcgcgcg
     1981 ggtcgatgac gtcgtcgatc atcgcgttgc ccgcggcgat gtagggatcg atcgtcgcac
     2041 ggatcgcgtc ctccatctgc ttgcgcgtcg cctccgggtc gtcggcagcc tcgatcagcg
     2101 agcggccgat gatgttgacc gcgccctccg cgcccatcac cgagatctcg gccgacggcc
     2161 aggcgacgat caggtccggc tcgtaggcct tgccgcacat cacgtagtag ccggcgccgt
     2221 aggccttgcg gacgaccacc gtgaccttcg gcacggtcgc gttcgagacg gcgtagagca
     2281 tcttcgcccc gtggcggatg atcccggcgt gctcgacctt ggtgccgacc atgaagccgg
     2341 gcacgtcctg caggaagagc agcggtatgc cgtaggcgtc gcagagattg acgaagcgcg
     2401 ccgccttgtc ggctgagtcg ttctcgagga tcccgccgag gtgctttggc tggttagcga
     2461 cgatgcctac cggtcgcccg cccatccgcg cgagacaggt gacgatcgtc cgagcgaagc
     2521 gcggcttgat gtcgaaccag acgccgtcgt cgacgatccg gcggatcacg tcgtacatgt
     2581 catagggctg gcggttcgac ggcggcagca cgtcgagcag ctcctcgtcc atgcggtcga
     2641 cggggtcggc cgtcgggcgg atcggcggcg cctgctcgca gttctgaggg aagtacgaga
     2701 ggtagtcctt gatcgccgcg atgcactcct cgtcggactt gacctcgagg tcgcctacgc
     2761 ctgagacctt ggtgtggatc ttcgagccgc cgagctcctc ctgggtcacg tcctcgccgg
     2821 tcaccgcctt gacgaggtgg ggccccgcga gggccatcga gccgcgaccc ttgaccatcg
     2881 gcacgaagtc cgcgagacct gggatgtagg cggtgccggc ggcgcacggc cccatcaacg
     2941 cggcgatctg cgggatcacg ccgctcatca cgacctcctc gcggaagagg aagccggagc
     3001 cggcgaacag cgagcctgcg gcctcctgga tccgcgctcc ggcggagtcg agcagccaga
     3061 tgaaggggat ccgcttctgc agcgcgagct cgcgcaggcg cgtgaccttg agctcgccgg
     3121 tcatgcccat cgaaccggcc atcaccgtga agtcgtaggc gcagacggcg accaggcggc
     3181 cgtcgacctt gccgtagccg gtgacgacgc cgtcggccgg cgcctccttg ccctccatcg
     3241 ctcgctgggc gaaatgcggc cggccgtgga tgccgagctc ggtgaagctg cccgcgtcga
     3301 tcaggaggtc gagccgctcg cgcgcggtca gcttgtcggc ggcgtgctgg cgctcgatct
     3361 tctccggccc accgccgagc ttcgccttct cgcgtcgggc gtggaggtcc tcgacgagtg
     3421 ggcgcagcag tgactcgggt ggggcgtcgg tcggcatgat cctccgtcag aagtaagaag
     3481 ctctactcct tactttgacg aatcgccggc ctaggcgctg gccgctcgct ccgggatcgc
     3541 ctcgagcagc ggctcgagca cgcgcatcac gaccgccgcg gcctcctggc ggtcctcctc
     3601 gtcctccggc gcctcaaacg tcgtgacctc gacgccgacc agctcgcagt ccgccatcac
     3661 cgcctcaatc aggtcgaaca gcttctccgg cgcgagcccc tccggcgccg gctcgcgcgc
     3721 cgggaagacg gccgggtcga tcacgtcgag atcgagatgg atgtagaccg gcgctccgtc
     3781 gagtgcgttc ttgaccgcga ccagcgtctc gaccgggctg gcgccgacga ccgtcacgtc
     3841 gctgcgctcg agcgccttgc gctcatcgcc gtcgaggtcg cgcacgcccg cgagcacaac
     3901 gcggtcctcg ggcaccgtgt cggcgatcag gccggcattc cagagcccgc aggcgccggc
     3961 gagcgccatc ccgctgaggc tgccgctgac cgtggtgtcg ggggtgttga agtcgccatg
     4021 ggcgtcgagc cagaggacga ccgtgtcggg acggtggcgc aggacagtcg gcagcgtggt
     4081 cagcgcgacc gagcactcgc cggcgagcaa caccggcagc cggccggcgg cgagtgcgtc
     4141 gtcgacctgc ccgcccgcct cgagcaggca gccgcgcgag tcgctcacgt cgtcctccca
     4201 gttcgcctcg cgcggctcgc ccggcgagcc gatctggcgc ggctcgcgcc cgagccgctt
     4261 gccgatcagc ggcgcaagct cgtcgacgcc gcgcacggcc gccgccgtcc ggtccgacgt
     4321 gcggcagcgc agcgccacca gcgagacgtt caccgcccgc tccactgcgg ctcacgcttc
     4381 tcgaagaacg ccttcacgcc ctcctggatg tcgtcggtcg agaacgacag cgtcaggttg
     4441 tggtgcaggt actcgagcgc gtcgtcgaag gccatgtcct gctggcggta catcgcgtcc
     4501 ttgccgagtc gcatcatcag cggcgacttc ttcgcgagct gttcggccca gaggcggacc
     4561 tcgtactcga gccccttggc cggcacgacc ttgttgacga gtccgatctc gagtgcctcc
     4621 ttcgcgctga cgcgctcgcc gagtagcagc agctcgttgg tcttcttgcg ccccacgttg
     4681 cggtagatca gcgccatgat catgaagggg aagaccccga cgttgatctc gggcgtgccg
     4741 aacgtcgcct tgtccgaggc cacgatcagg tcgcaggcga gcgcgacgcc gagcgagccg
     4801 gcgagcacat ggccgttcgc cgcgcagatc gtcggcttgc cgagctggcc gatcgtgcgg
     4861 aacagccgcg ggaacagctc agtgccgaag tgcttgtgga cgagcggcat ctcggccgcg
     4921 aagccggcga gattgccgcc ggccgagaac accttgtcgt gcgaggaggt gagtacgacg
     4981 cagcgcacgt cgtcgtcggc gcgcgcctgc tcgaaggccg cgatcagctc ggtgagcgtg
     5041 tcgctaccga gcgcattgcg cgtctcgggg tcgtcgagcg tgatcgtcgc gactttcgcc
     5101 tcgtcgacct cgtaacggat cttctcgaag gccactgcgg cgagattatc cgggcaccgg
     5161 catccacgcc gcccccatgt cgacgcgtgc gccgcggaac gggacgccct cggcctcgag
     5221 caggccgcgc tggcggtcgc ccttcggcag cgagccgtcg gcgcggacga cccgctgcca
     5281 cggcaggtcg ggatcgtcgc aggccgagag caccgagccg gcgaagcgcg gcgcgcccgg
     5341 cgagaggtcg ccgtaggtcg tcacgcggcc gggcgggatc gcgcgcacgc gctcgagcac
     5401 cgccgtggca cgcggcgagt cgatcagagc ccgtactcct cgagcaggtt cagccacagc
     5461 tcgctgcgcg tcgggaacgc cggaaccgca tgccagaggc ggtcgagcgg cagctcgccg
     5521 acgaccgcga tcgtcgcggc gtgcagggac tctgcgacct cggcgccggt gaaggtggcg
     5581 cccacgatca cgcgccggtc ctcgtcgacg acgatccggg cggtgccagc ggtgccacga
     5641 ccgacaaagc tgccgccggc gttggcctcg gtcccgacgt cgaccgcgcg cacgttgagg
     5701 ccggcctcct gcgccgaggc cagcgtgtgg ccgacggcgg cgacctgcgg gtcggtgaac
     5761 acgacacgcg gggagaccgg cccgtcctcg gtcaccgcgg cgtgctttcc gaggatcacg
     5821 tcggccgccg cgcgcgcctg atacttgccc atgtgggtga gcagcgcgcg gccgttgacg
     5881 tcgcccacgg cgtagagcca gtcgccgtcg atgccggtcg cgcgcatctg gtcgtcgacg
     5941 tcgatcgact cgccgggact caggccgacg ctctcgaggt tgatgtcgtc ggtgcgcgga
     6001 cggcggccgg cggcgacgag cagctcgtcg gcgatcacgg gggggtcccc ctcgagctcg
     6061 accgccactg cgccggtctc ggcgcgcacc gcggcgaccg tcgcctccag gcggacgtcg
     6121 actccctggg cacgcaggga gtcg
//
LOCUS       DATABV010000136         7426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2371282, whole genome shotgun sequence.
ACCESSION   DATABV010000136 DATABV010000000
VERSION     DATABV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371282"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..250)
                     /locus_tag="VHF88_05785"
     tRNA            complement(179..250)
                     /locus_tag="VHF88_05785"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Gln,seq:ttg)
     gene            302..1678
                     /gene="glmU"
                     /locus_tag="VHF88_05790"
     CDS             302..1678
                     /gene="glmU"
                     /locus_tag="VHF88_05790"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX2161317.1"
                     /translation="MAEQLTVLVMAAGKGTRMRSALPKVLHPICGRPMVEWVIEAARV
                     AGATDVVCVTRPGDGVAEGLPDGVRVAEQTEGEGTGAAVLAAREAVAAGGDVMILSGD
                     QPLISAELLRELVATHDVAGAAATLLTTEGLDPTGYGRIVRDADGGVERIVETKDPSG
                     VAPEALAIREINLGTYVFESDALFAALDAVGLESGETYLTGVFPRLREQGRNVVAHVT
                     DDLSSAVGVNSRVDLMDAERLAQRRILERHALAGVSFLAPDSTRVEAGVEIGGDTTIG
                     PGVTLRGDTRIGAGCEIGPQTTITDSSLADGVACVHSFVTGARIESGGSVGPFAYLRP
                     GAHIGEGAKVGTFVEIKNSEIGAGAKVPHLSYLGDADVGDGANVAAGNITANYDGFRK
                     HRTKIGAGARTGVNSSFVAPVTVGDGAYIGAGSVISEDVPPGALGISRPEQRNVDGYT
                     ERIERERG"
     gene            1675..2706
                     /locus_tag="VHF88_05795"
     CDS             1675..2706
                     /locus_tag="VHF88_05795"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX2161318.1"
                     /translation="MSAVSTETEFTAGTYIPASYDKRLMVAAGRGTQALGAKIADNLG
                     VDLADAGLKTFSDGEVYCRYTDSIRGADLFIVASIAGSEDEGMTVNDALMELLVMIHA
                     AKHASAHRIIAVTPWYGYSRQDKKSAPREPISARLVAEVLETAGIDRLVTMDLHAGQM
                     QGFFSKPVDHMTAMPILTQFVADQLAGEDLVIIAPDAGRVKLVRKFAQKVGAPYALLE
                     KERPSHQVAEIGYVIGDVQDKVAVIVDDIIDTGGTLSAAAQTVLDEGASKVYAVATHG
                     VFSGRAFETLEASPLAGIVVTDTVPLRDGAPERIRVLTSADILTDSIRRIFLDDSVSE
                     IFEGENQLF"
     gene            2749..3204
                     /locus_tag="VHF88_05800"
     CDS             2749..3204
                     /locus_tag="VHF88_05800"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX2161319.1"
                     /translation="MYDLLLTIHVLAAVVWVGGNISLHIFGRRVLKRGNGEEIYKFSN
                     DINTIALRLYAPTSLILLVAGILLVNEVGYDFGDLWITLAFLGWIISFILGIGYYGPQ
                     DKKLQALVAAEGPTAPGVAANARQALFVNQIELLILVLVVMDMTVKPGL"
     gene            complement(3205..4638)
                     /locus_tag="VHF88_05805"
     CDS             complement(3205..4638)
                     /locus_tag="VHF88_05805"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX2161320.1"
                     /translation="MAKEDESIPTGRVRRTAQVGSVIGSQGARWAGTRAANLARSKEQ
                     GAAQLDKRHMEAAERMVDTLGTMKGAAMKIGQLASFIDTDFLPEEYRELYQDKLSNLR
                     SEAPAMPWKKVRGVLEQEWDEPVEELFEDFEHEAAAAASIGQVHRATLPDGRAVAVKI
                     QYPGVAEALRADMQNAGMIMRLAKAIAPGLNAKAAAAELKERVLEELDYEYEAQNQRA
                     FSRGYRGHPFIYVPDVVTRLSRGRVLVTEWVDGVGFDDVKALPQAERDRFAEIVFRFC
                     FGSIYHLQHFNADAHPGNYLLMDDGKVAFLDFGMTKQLDKEQIELEIAALDAVFAGDP
                     ERLRTAMGDLGFVSNPRKVDAERLMAHVITVGGWYMQDREVTINSRRVMEAITAVSDP
                     RSEFFDLLRRENIPANELMGRRMEVGVLAVLGQLEATRNWYRIGREWWFGDEPATELG
                     REEWAYFESRGEKRVRKFSARGRSGGA"
     gene            4686..5363
                     /locus_tag="VHF88_05810"
     CDS             4686..5363
                     /locus_tag="VHF88_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX2161321.1"
                     /translation="MASDRPVLAVETREERGSRSSRRLRRAGLVPGVLYGGSHDEPVS
                     FKVGDRDLRRILNEGAALIDVRIGKDKSVPAIVKDRQLHPVRDEVVHIDFLEVNLKEK
                     IRAPVALELEGVDDTPGVKEGGVLDHATREVEIEALPTDIPEQIVVDVSGLEIGATIT
                     LADIEAPDGVEFVHEHLDEVVIASVVLPTKVEEPEIEAETEVVGEGGEAAEDAEDSGG
                     DDSGGGE"
     gene            5373..5978
                     /gene="pth"
                     /locus_tag="VHF88_05815"
     CDS             5373..5978
                     /gene="pth"
                     /locus_tag="VHF88_05815"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX2161322.1"
                     /translation="MGLFRRREEGDGGRVDWLVVGLGNPGRKYEGTRHNVGFEVAALA
                     AQRWDLPKAKQKFAGLYTEGRTGPGGPRVGLLLPQTFMNESGRSAGPARGSLHVPLDR
                     VVAVHDEIDLPFGRIHSRVGGGLAGHNGLKSLDRELGGRDFRRVRVGVGRPDSTDPSI
                     VVPYVLGRFSEPKEDVARLIEDAVDELDRLVSSDSAELSAP"
     gene            6027..>7426
                     /locus_tag="VHF88_05820"
     CDS             6027..>7426
                     /locus_tag="VHF88_05820"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161323.1"
                     /translation="MSLRPLLTYAQEDEAVAALSDAARKAPQRAFVSQSMRPYLLAAL
                     LDAQPERPALVVAGDDRAARDLAADLKQFLSPRPVRLYPARGVRYESHLAPPPHLVGL
                     RIAALDALVEGSAVAGGAPGAGAPVVVAGAVALAEKVPDPELRPHGFTIEKGGLLDLD
                     ETAAQLVACGYERVEQVEERGQFALRGDIVDIYPATEERAVRCELFDIEVERLTFFST
                     FTQRSLEEVERVEIAPAAELAPEHRELAEIAAADIAAQSRKKSDDDSGTDSPGDDRLD
                     IAELLPVDRFGELLDLLPADALVAIAAEEELAPALRDHWDDVTTSFHDADAGHLYVDP
                     ERLQEGLDARAALRLSSISGDQPHSFRAQAAETAARSLEAAEPELEKLIRSGYRTFVP
                     WARQGEAERAAYNLTRVRAAFLGGAPRARPGSAAADDVPELRAKARAADVEAPHEPGL
                     TFAVASLREGFVAPAR"
BASE COUNT         1080 a         2714 c         2580 g         1052 t
ORIGIN      
        1 ccaccgccac acgatccgct accgcctcga ccgcgtccgc gagctcacca acctcgacgt
       61 cgggtccacc gacggccgcg agcgcctctc cctcggcctc aaggccatgc gcgtcctcgg
      121 catctcaccc cccggcggcc cagcccagga gttgcggatc cgagactgac gaagcgcact
      181 ggggagcgcg gatttgaacc gcgacttcaa ggctccaaag gcctgcgtgc tgccgttaca
      241 ccactcccca aggggccgcg tcaggatagg cgaggcggtg cgccggtacg ctgcgcgggt
      301 catggccgaa cagctgactg tccttgtcat ggccgcgggc aagggcacgc gcatgcgctc
      361 ggctctgccc aaggtcctgc atccgatttg cgggcggccg atggtcgaat gggtgatcga
      421 ggcggcgcgg gtggcgggag cgaccgacgt ggtctgcgtg acgcggcccg gcgacggggt
      481 ggccgaggga ctgcccgacg gcgtcagggt cgccgagcag acggaggggg aggggaccgg
      541 cgcggcggtg ctggcggcgc gtgaggccgt cgcggcgggt ggcgacgtga tgatcctgtc
      601 gggcgatcag ccgctgatct cggccgagct gctgcgcgag ctggtggcga cccacgacgt
      661 tgcaggcgcc gcggcaacgt tgttgacgac cgagggactc gaccccaccg gctacgggcg
      721 gatcgtgcgc gacgccgacg gcggcgtcga acggatcgtc gagaccaagg acccgagcgg
      781 cgtcgcgccc gaggccctcg cgatccgcga gatcaacctc ggcacctacg tcttcgagtc
      841 cgacgcgctc ttcgcggcgc tcgacgccgt tgggctcgag agcggcgaga cctacctgac
      901 cggcgtcttc ccgcgtctgc gcgagcaggg ccggaacgtc gtcgcgcacg tcaccgacga
      961 cctcagcagc gccgtcgggg tcaacagcag ggtcgacctg atggacgccg agcggctcgc
     1021 ccagcggcgg atcctggagc gccacgcgct cgccggcgtc agcttcctcg cccccgattc
     1081 gacgcgggta gaggcgggcg tcgagatcgg cggcgacacg acgatcgggc cgggcgtcac
     1141 gctgcgcggc gacacgcgga tcggcgcggg ctgcgagatc ggcccccaga ccacgatcac
     1201 cgactcgtcg ctcgccgacg gcgtcgcctg cgtccactcg ttcgtcaccg gcgcccggat
     1261 cgagtccggc ggctcggtcg gtccgttcgc ctatctgcgc ccgggcgccc acatcggcga
     1321 gggggcgaag gtcggcacct tcgtagagat caagaactcg gagatcggcg ccggcgccaa
     1381 ggtgccgcac ctctcctacc tcggcgacgc cgacgtcggc gacggcgcca acgtcgcggc
     1441 gggcaacatc acggcaaact acgacggctt ccgcaagcac cgcacgaaga tcggcgcggg
     1501 ggccaggacg ggggtcaaca gctcgttcgt cgcccccgtg accgtcggcg acggggcgta
     1561 tattggggcc ggatcggtga tctccgagga cgttccgccc ggcgcgctcg gaatctcgcg
     1621 gccagagcag cgcaacgtcg acggctacac ggagcggatc gagagagagc ggggatgagc
     1681 gccgtcagca ccgagaccga gttcaccgcg gggacctaca tcccggcgag ctacgacaag
     1741 cggctgatgg tcgccgccgg ccgcggcacc caggcgcttg gcgcgaagat cgccgacaac
     1801 ctcggcgtcg acctcgccga tgccggcctc aagacgttct ccgacggcga ggtctactgc
     1861 cgctacacgg actcgatccg cggcgccgac ctcttcatcg tcgcctcgat cgccggcagc
     1921 gaggacgagg gcatgacggt caacgacgcg ctgatggagc tgctggtgat gatccacgcc
     1981 gccaagcacg cctcggcgca ccggatcatc gccgtcacgc cgtggtacgg ctactcgcgt
     2041 caggacaaga agtccgcgcc gcgcgagccg atctccgccc gcctcgtggc cgaggtgctc
     2101 gagaccgccg gcatcgaccg gctcgtgacg atggacctcc acgccggcca gatgcagggg
     2161 ttcttctcga agccggtcga tcacatgacc gcgatgccga tcctgaccca gttcgtcgcc
     2221 gaccagctcg ccggcgagga cctcgtgatc atcgccccgg acgccggccg cgtgaagctc
     2281 gtgcgcaagt tcgcccagaa ggtcggcgcg ccctacgcgc tgctcgagaa ggagcggccg
     2341 agccaccagg tcgccgagat cggctacgtg atcggcgacg tccaggacaa ggtcgcggtg
     2401 atcgtcgacg acatcatcga caccggcggc acgctcagcg ccgcggccca gaccgtgctc
     2461 gacgagggcg cctcgaaggt ctacgcggtc gccacccacg gcgtcttctc cggccgcgcg
     2521 ttcgagacgc tcgaggcatc gccgcttgct gggatcgtcg tcaccgacac cgttccgctg
     2581 cgcgacggag cgcccgagcg gatccgcgtg ctgaccagcg ccgacatcct caccgactcg
     2641 atccgccgca tcttcctcga cgactccgtc tcggagatct tcgagggcga gaaccagctc
     2701 ttctagcgga gccagcaggc gtccgccggc cggattaggc tcgcgcccat gtacgacctt
     2761 ctgctgacga tccacgtcct ggccgccgtc gtctgggtgg ggggcaacat ctcgctgcac
     2821 atcttcggcc ggcgggtgct caagcgcggc aacggcgagg agatctacaa gttctcgaac
     2881 gacatcaaca cgatcgccct gcgcctctac gcgccgacgt cgctgatcct gctcgtagcc
     2941 ggcatcctgc tcgtcaacga ggtcggctat gacttcggcg acctctggat cacgctcgcc
     3001 ttcctcggct ggatcatcag cttcatcctc ggaatcggct actacggccc gcaggacaag
     3061 aagctgcagg cgctggtcgc ggccgagggc ccgaccgctc caggggtcgc cgcgaacgcc
     3121 cgccaggcgc tgttcgtcaa ccagatcgag ctgctgatcc tcgtcctggt agtgatggac
     3181 atgaccgtca agcctgggct ctagctaggc gccgccggag cgcccgcgcg ccgagaactt
     3241 gcgtacgcgc ttctcgccgc gcgactcgaa gtaggcccac tcctcgcggc ccagctcggt
     3301 cgccggctcg tcgccgaacc accactcccg gccgatcctg taccagttgc gcgtcgcctc
     3361 gagctggccg agcacggcga gcacgccgac ctccatccgg cggcccatca gctcgttggc
     3421 agggatgttc tcgcgccgca gcaggtcgaa gaactccgag cgcgggtccg agacggcggt
     3481 gatcgcctcc atcacccggc gcgagttgat cgtcacctcg cggtcctgca tgtaccagcc
     3541 gccgacggtg atcacgtgcg ccatcagccg ctcggcgtca accttgcgcg ggttgctgac
     3601 gaagccgagg tcgcccatcg ccgtccgcag ccgctcgggg tcgccggcga acacggcgtc
     3661 gagcgccgct atctcgagct cgatctgctc cttgtcgagc tgcttggtca tcccgaagtc
     3721 gaggaaggcg accttgccgt cgtccatcag caggtagttg ccgggatggg cgtcggcgtt
     3781 gaagtgctgc aggtgataga tcgagccgaa gcagaagcgg aagacgatct cggcgaagcg
     3841 gtcgcgctcg gcctggggca gcgccttgac gtcgtcgaag ccgacgccgt cgacccactc
     3901 ggtgacgagc acgcgcccgc gcgacagccg cgtgacgacg tcgggcacgt agatgaacgg
     3961 gtggccgcgg tagccgcgcg agaaggcgcg ctggttctgg gcctcgtact cgtagtcgag
     4021 ctcctcgagc acgcgctcct tgagctcggc ggccgccgcc ttggcgttga gccccggcgc
     4081 gatcgccttc gcgagccgca tgatcatccc ggcgttctgc atgtcggcgc gcagcgcctc
     4141 ggcgacgccg gggtactgga tcttcaccgc gacggcacgg ccgtcgggca gggtggcgcg
     4201 gtggacctgg ccgatcgagg ccgcggcggc ggcctcgtgc tcgaagtcct cgaacagctc
     4261 ctcgacgggc tcgtcccact cctgctcgag caccccgcgg accttcttcc agggcatggc
     4321 gggcgcctcg gagcgcaggt tcgacagctt gtcctggtac agctcgcggt actcctcggg
     4381 caggaagtcg gtgtcgatga acgaggcgag ctggccgatc ttcatcgccg cgcccttcat
     4441 cgtcccgagc gtgtcgacca tccgctcggc ggcctccata tggcgcttgt ccagctgggc
     4501 cgcgccctgc tccttcgagc gcgcgaggtt cgcggcccgg gttcccgccc agcgcgcccc
     4561 ctgactgccg atcaccgagc cgacctgcgc ggtccgccgc acgcggcccg tcggtatcga
     4621 ctcgtcctcc ttggccacgg cgcgaaccat agagcgcccg agcgagctag actggccggc
     4681 cgatcatggc ttccgaccga ccagttctcg cagtggagac ccgtgaggag cgcggctcgc
     4741 gctcctctcg ccgtctgcgc cgcgccggcc tcgtgcccgg cgtgctctac ggcggctcgc
     4801 acgacgagcc ggtctcgttc aaggtcggcg accgcgacct gcgccgcatc ctcaacgagg
     4861 gcgcggcgct gatcgacgtc cggatcggca aggacaagtc ggtcccggcg atcgtcaagg
     4921 accgccagct gcaccccgtt cgcgacgagg tagttcacat cgacttcctc gaggtgaacc
     4981 tcaaggagaa gatccgcgcc cccgtcgcgc tcgagctcga gggcgtcgac gacacccccg
     5041 gcgtcaagga gggcggcgtg ctcgaccacg cgacgcgcga ggtcgagatc gaggcgctgc
     5101 cgaccgacat tcccgagcag atcgtcgtcg acgtgtccgg gctcgagatc ggcgcgacga
     5161 tcacgctcgc tgacatcgag gcgcccgacg gcgtcgagtt cgtccacgag cacctcgacg
     5221 aggttgtgat cgccagcgtc gtgctgccga ccaaggtcga ggagccggag atcgaggccg
     5281 agaccgaggt cgtcggcgag gggggcgagg cggccgagga cgccgaggac tccggcggcg
     5341 acgattcggg cgggggcgag tagcgccgca cgatggggct gttccgccgt cgggaggagg
     5401 gcgacggggg cagggtcgac tggctcgtcg tcgggctcgg caacccgggc cgcaagtacg
     5461 agggcacgcg ccacaacgtc ggcttcgagg tcgccgcgct ggccgcgcag cgctgggacc
     5521 tgccgaaggc gaagcagaag ttcgccggcc tctacacgga gggccgcacg ggtccgggcg
     5581 gcccgcgcgt gggcctgctg ctgccgcaga ccttcatgaa cgagtcgggc cgctccgcgg
     5641 gcccggcacg cggttcgctg cacgtgccgc tcgaccgcgt cgtcgccgtc catgacgaga
     5701 tcgacctgcc gttcggtcgc atccactccc gcgtcggcgg cggcctcgcc ggccacaacg
     5761 gcctcaagtc gctcgaccgc gagctcggcg gccgcgactt ccgccgcgtc cgcgtcggcg
     5821 tcgggcgccc cgactcgacc gacccctcga tcgtcgtccc ttacgtcctc ggccgcttca
     5881 gcgagcccaa ggaggacgtc gcgcgcctga tcgaggacgc cgtcgacgag ctcgaccggc
     5941 tcgtcagctc ggattccgcc gagctgagcg ctccctagaa tcggagcagg ccgcgcggct
     6001 gtgtccgggc ggccgtttcg cgtgccatgt cgctgcgacc actgctcaca tacgcccagg
     6061 aggacgaggc cgtcgcggcg ctgtccgacg ctgcgcgcaa ggcgccgcag cgggcgttcg
     6121 tgtcccagag catgcggccg tacctgctcg cggccctgct cgacgcgcag ccggagcggc
     6181 cggcgctggt ggtcgcgggc gacgaccgtg ccgcacgcga cctggccgcc gacctcaagc
     6241 agttcctctc cccgcggccg gtacggctct acccggcgcg cggcgtgcgc tacgagtctc
     6301 acctggctcc gccgccgcac ctcgtcggcc tgcggatcgc cgcgctcgac gcgctcgtcg
     6361 agggctcggc ggtcgcgggt ggcgcccccg gcgcgggcgc cccggtcgtc gtagccggcg
     6421 cggtcgcgct cgccgagaag gttcccgacc cggagctgcg cccgcatggc ttcacgatcg
     6481 agaagggcgg gctgctcgac ctcgacgaga ccgccgcgca gctggtcgcc tgcggctacg
     6541 agcgcgtcga gcaggtcgag gagcgcggcc agttcgcgct gcgcggcgac atcgtcgaca
     6601 tctacccggc gaccgaggag cgcgcggtgc gctgcgagct gttcgacatc gaggtcgagc
     6661 ggctgacctt cttctcaacc ttcacccagc gctcgctcga ggaggtcgag cgggtcgaga
     6721 tcgcccccgc ggccgagctc gcgcccgagc atcgggagct ggcggagatc gcggcagccg
     6781 acatcgcggc gcagtcgcgc aagaaatccg acgacgacag cggcaccgac agccccggcg
     6841 acgaccgcct cgacatcgcc gagctgctcc cggtcgaccg cttcggcgag ctgctcgacc
     6901 tgttgcccgc cgacgcgctc gtcgcgatcg ccgccgagga ggagctcgcc cccgcgctgc
     6961 gcgaccactg ggacgacgtg acgacgagct tccacgacgc cgacgccggc cacctctacg
     7021 tcgatcccga gcggctgcag gaggggctcg acgcccgcgc ggcgctgcgc ctctccagca
     7081 tctccggcga tcagccgcac agcttccgcg cccaggcggc ggagaccgcc gcacgctcgc
     7141 tcgaggccgc cgagcccgag ctcgagaagc tgatccgctc cggctaccgg acgttcgtcc
     7201 cgtgggcgcg ccagggcgag gcggagcgcg ccgcctacaa cctcacccgc gtccgcgccg
     7261 ccttcctcgg cggggcgccg cgcgcccgcc ccggcagcgc cgccgcggac gacgtccccg
     7321 agctgcgcgc gaaggcgcgt gccgccgacg tggaggcccc gcacgagccc ggcctgactt
     7381 tcgccgtcgc ttcgctgcgc gagggcttcg tcgcgccggc gcggaa
//
LOCUS       DATABV010000137         6020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2773682, whole genome shotgun sequence.
ACCESSION   DATABV010000137 DATABV010000000
VERSION     DATABV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773682"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VHF88_05825"
     CDS             <1..1071
                     /locus_tag="VHF88_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161324.1"
                     /translation="AQLLAQYQARLEQLVAAWDPTAQTTSPDFVDRRDLGTIGRYVNA
                     YKAAGGGWGDRVAFHPYWGAHAETTATTLDLIGLAPAGASVWITEVGAFARDQGSIEA
                     DETTQNDKVWWLAENLGTLPQVERIHYYHMRGNPNASWDTGLMDVDASPRIAWHTWCA
                     MSRGFTHPDCQPVTSRTAPPPPPPPPPSPDPPAPDPPPSTPAPSPGASQTPPSAPAPV
                     LPPGTSPRPAAPTATTPLRAPAGDRTPPALTIERLARRLTYAEFVDGIAVKVRSNEPT
                     TLVVTITVQGARRRDGRRRHAVVARKRVSAARVVRVVRLEPRKRRLGERRGFVARIEV
                     RATDLAGNGNRAISRVRVRRTG"
     gene            1155..3173
                     /locus_tag="VHF88_05830"
     CDS             1155..3173
                     /locus_tag="VHF88_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX2161325.1"
                     /translation="MEAVASRQSPRFRQLRVGFAVLAAGWLLYLVLIVSDAAVERELL
                     GLPLGDPIYAALLMGAGLLCLARATVDSEERRIWLAFGAGLTLWSFGDLWWMAFLATD
                     AAPPYPSVADALWLSSYVPMAYGIWSLISSRVSWRALGAAAWLDGAIAALAGSAVFAA
                     TLLAGPLAAAVQGEPMTFATNLAYPIGDLVLLGCVLAALSATGWRPGRAIALIAAGLL
                     LRALADFVYLDQVTQGTYAGGLLDTLWPAASLLVAAAACLPARSTRREPDWRAFLAPA
                     AFVTTALGLLLFDHFERLPSSAVVLAAVAVAVAAIRMVTMVRATLASTQRDALTDALT
                     GMKNRRSLERDLSRALEDLQPGQRLVFAMFDLNGFKRYNDNFGHPAGDSLLARLGTRL
                     AEAARPGSAYRLGGDEFCVLLPDAGDAEERLARADAALVDQGDGFKISAARGTASIPD
                     EASDPEEVMHLADRRMYAAKLGGRADDHTVEALLRALYEAKPRLEGDLGTIAVLARGV
                     AQRLGLIPEEIDEVVRAAKLHDVGKMAIPDSILNKPSPLDPDEWEYMRKHPVIGERIV
                     AAATPLLPVARLVRSSHERWDGAGYPDGLAGEAIPRGARIVFACDSFEAMTSGRPYRT
                     ARTPAEALAELRRCSGTQFDPEVVDALEAVVTERADVTRRAAPRTPSW"
     gene            complement(3136..4458)
                     /locus_tag="VHF88_05835"
     CDS             complement(3136..4458)
                     /locus_tag="VHF88_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX2161326.1"
                     /translation="MAVRRESGQALVLMLGVVAAVLVGALVLVALGQALGGKSRPQRA
                     ADLAAVSAAHRMSDDYFRLFEPAVLPNGLPNPSHLSTDEYLARARSVALRAGARNGVR
                     IAAADVSFPDGGFAPTRVRVRARGDVEVRVGRRSARRRIDVEASATAELAVLAGAPGM
                     PTMASGGGYSGPLAYRGGKPMRPDVAIAFDRMNAAARADGISLLVVSGFRSDAEQAAL
                     FAANPDPKWVAPPGKSLHRLGTELDLGPDSAYGWLAANASRFGFVKRYAWEPWHFGYV
                     RSAGSASLGYGAAIRDGRSAIPAFVPREFVDEISRASQRWNVGAAVLSAQLYAESNFN
                     PFATSSAGAQGIAKFMPGTAASYGLSNPFDPAAAIDAQAHLMHDLLRQFASVPLALAA
                     YNAGPAPVAACGCVPSYPETQAYVAKILGLLDGAGDLTSSAFEVRLVE"
     gene            complement(4461..5210)
                     /locus_tag="VHF88_05840"
     CDS             complement(4461..5210)
                     /locus_tag="VHF88_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161327.1"
                     /translation="MTASAREQSDLVAARRRGLLSAVAEFLVEPIEPRTPVDARAERH
                     WTDAPRPTVAVTGLASRCGTTTVARALAAELALRDSEGAAIVSADAIAGGGIPLGTPA
                     AGRLGRAVQRSLPSQTRAVGRLCLAAAGPDVAGLVDAARDLAPLVLDVTDSRDAAAAA
                     SLVDTVVLVGSPAVEPALAAVLSDSLSRVGPEPVVVLNRDRGEERWDGRCALRLPEAR
                     MGGQLALAGREARGELGKAVAKLADLLAVAP"
     gene            complement(5207..5548)
                     /locus_tag="VHF88_05845"
     CDS             complement(5207..5548)
                     /locus_tag="VHF88_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161328.1"
                     /translation="MTGLLGEERGQALVEVVAGLPAMLAIGLVMLQLLAVGYSAVLAG
                     VAAEAGALALAGGDDAGAAVKRSLPGWSEARARVEVSGGTVRVLLRPPAPLALVARKL
                     EVDADATVELP"
     gene            complement(5545..5898)
                     /locus_tag="VHF88_05850"
     CDS             complement(5545..5898)
                     /locus_tag="VHF88_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX2161329.1"
                     /translation="MGIARRLRRSERGQASVELVAALPFVLLVGAVVWQCALAGHTAW
                     LAAHAARAGARADIVDGDVREAVRAALPDGLEHGLGVERDDGLVRVSVDLPLLLHRWR
                     LPVAVGATASLGERR"
BASE COUNT          837 a         2209 c         2191 g          783 t
ORIGIN      
        1 gcccagctgc tcgcgcagta ccaggcgcgc ctcgagcagc tggtcgccgc ctgggatccg
       61 actgcgcaga ccacctcgcc ggacttcgtc gaccgccgcg acctcgggac gatcggccgc
      121 tacgtgaacg cctacaaggc cgccggggga ggctggggcg accgcgtcgc gttccacccc
      181 tactggggcg cgcacgccga aacgaccgcg acgacgctcg acctgatcgg tctcgcgccg
      241 gcgggggcga gcgtctggat caccgaggtc ggcgccttcg cccgcgacca gggctcgatc
      301 gaggcggacg agacgactca aaacgacaag gtctggtggc tcgccgagaa cctgggaacg
      361 ctgccgcagg tcgagcggat ccactactac cacatgcgcg gcaacccgaa cgcgtcctgg
      421 gacacgggcc tgatggacgt cgatgcgagc ccgcggatcg cctggcacac ctggtgcgcg
      481 atgtcgcgcg gcttcaccca tcccgactgc cagcccgtga catcccggac cgcgccgccg
      541 ccaccgcctc ccccaccgcc ttcacccgat ccgcctgcgc cggacccgcc accgagcacg
      601 ccggcacctt cgccgggcgc gtcgcaaacg ccgccttccg cgcccgcgcc agtcctccca
      661 ccggggacta gcccgcgtcc ggcagcgccg acggcaacga cgcctctccg ggcaccagcg
      721 ggcgatcgga ccccgccagc gttgacgatc gagcgtctcg cccgcagact gacctacgcc
      781 gagttcgtcg acggcatcgc ggtgaaggtg cggtcgaacg agccgacgac cttggtcgtc
      841 acgatcaccg tgcagggcgc tcggcgacgc gacggccgcc gccggcacgc cgtcgtggcg
      901 aggaagcggg tctcggcggc ccgcgtcgtc cgcgtggtcc ggctcgagcc gaggaagagg
      961 cggcttggcg agcgacgcgg cttcgtggca cggatcgagg tccgagcaac ggacctcgcc
     1021 gggaacggga atcgggccat cagccgggtc cgcgtgcgcc gcacgggctg atcgatagcg
     1081 gtcgcgatat cgggcgactc gatgttgccc tacggcccgt aaagggttga agaaccgatg
     1141 tcgatggagg tcgcatggaa gccgtcgcat ctcggcagag tccccgcttt cgccagctgc
     1201 gcgtcggctt tgccgtcctc gccgcggggt ggctgctcta cctggtcctg atcgtcagcg
     1261 acgccgcggt cgagcgcgag ttgctggggc tgccgctcgg cgacccgatc tacgccgccc
     1321 tgctcatggg tgccggcctg ctgtgcctgg cccgtgcgac ggtggactcc gaggagcgcc
     1381 gtatatggct ggcgttcggt gccgggctca cgctctggtc gttcggcgac ctgtggtgga
     1441 tggcgttcct ggcgacggat gccgcgccgc cctacccgtc ggtcgccgac gccctctggc
     1501 tgtcgtcgta cgtgccgatg gcgtacggca tctggtcgct catctcgtcg cgcgtgagtt
     1561 ggcgggcgct tggcgccgcg gcctggctcg acggcgcgat cgccgcgctg gcgggcagcg
     1621 ccgtgttcgc agccaccctt ctcgccgggc cgctggccgc tgccgtccag ggcgagccga
     1681 tgaccttcgc cacgaatctc gcgtatccga tcggcgatct cgtcctgctc ggctgcgtgc
     1741 tcgccgccct gtccgcgacg ggctggcggc cggggcgggc gatcgcgctg atcgccgcgg
     1801 gcctgctgct acgggcgctc gccgacttcg tctatctcga tcaggtcaca caaggaacct
     1861 atgcgggcgg gctgctcgac accttgtggc cagctgcctc gctcctcgtc gcagccgctg
     1921 cttgcctccc cgcgcgcagc acccggcgtg agcccgactg gcgggccttc ctggccccgg
     1981 ccgccttcgt gacgaccgcg ctcggattgc tgctgttcga ccacttcgag cggctgccgt
     2041 cctcggcggt cgtcctggcg gcggtcgccg tcgcggtcgc ggccatccgc atggtgacga
     2101 tggtgcgcgc cacgttggcc tcgacgcagc gcgacgcgct gacggacgcg ctcaccggca
     2161 tgaagaaccg ccgcagcctc gagcgagacc tgtcgcgggc gctcgaggac ctccaaccgg
     2221 gccagcgcct cgtgttcgcg atgttcgacc tcaacggctt caagcgctac aacgacaact
     2281 tcggccaccc ggccggcgac tcgctgctgg cgcggctggg cacccgcctc gccgaggccg
     2341 cccgcccggg cagcgcctac cgcctcggcg gtgacgagtt ctgcgtcctg ctgcccgacg
     2401 ctggcgatgc ggaggagcgg ctcgcgcgcg ccgacgccgc gctcgtcgac cagggcgacg
     2461 ggttcaagat ctcggccgct cgcgggactg cgtcgattcc tgacgaggcg tccgaccctg
     2521 aggaggtcat gcacctggcg gatcgccgga tgtacgccgc caagctcggg ggacgcgcag
     2581 acgaccacac cgtagaggcg ctgctgcgcg cgctctacga ggccaagccg aggctggagg
     2641 gggatctggg caccattgcc gtgctcgcgc gtggagtggc gcagcggctc gggctgatcc
     2701 ccgaggagat agacgaggtg gtgcgcgccg cgaagctgca cgacgtcggc aagatggcca
     2761 tcccggactc gatcctcaac aagccgtctc cgctcgatcc cgacgagtgg gagtacatgc
     2821 gcaagcaccc cgtgatcggc gagcggatcg tcgccgccgc cacgccgctg ctgccggtgg
     2881 caaggctcgt gcgatcgagc catgagcgct gggacggcgc cggctatccg gatgggctgg
     2941 ccggcgaggc gataccccgc ggcgcccgga tcgtgttcgc ctgcgacagc ttcgaggcga
     3001 tgaccagcgg gcgcccctac agaactgccc gcacgccagc ggaggcgctc gcggagctgc
     3061 gccgctgcag tggcacccag ttcgatcccg aggtcgtcga tgcgctggag gccgtcgtga
     3121 cagagcgcgc ggacgtcact cgacgagccg cacctcgaac gccgagctgg tgaggtctcc
     3181 ggcgccgtcg aggagtccga ggatcttggc gacgtaggcc tgggtctctg ggtaggacgg
     3241 gacgcagccg caggcggcga ccggtgccgg gccggcgttg taggcggcga gggcgagcgg
     3301 gactgaggcg aactgacgca gcaggtcatg catcaggtgg gcttgggcat cgatcgcggc
     3361 ggcgggatcg aaggggttgg agaggccgta gctggcggcg gtgccgggca tgaacttcgc
     3421 gatgccctgg gcgcccgcgg aggaggtcgc gaacgggttg aagttcgact cggcatagag
     3481 ctgggctgag aggaccgcgg cgccgacgtt ccagcgctgg gaagcgcgcg agatctcgtc
     3541 gacgaactca cgtggcacga acgccgggat cgccgaacgg ccgtcgcgga ttgcggcgcc
     3601 gtagccgagc gacgccgagc cggccgagcg gacgtagccg aagtgccacg gctcccaggc
     3661 gtaccgcttc acgaatccga agcgtgaggc gttggcggcc agccagccgt aggcggaatc
     3721 ggggccgagg tcgagctcgg tgcccaggcg gtggaggctc ttgccgggcg gggcgaccca
     3781 cttgggatct gggttcgcgg cgaagagcgc ggcctgctcg gcgtcggagc ggaagccgct
     3841 gacgacgagc agggagatgc cgtcggcgcg ggcggcggca ttcatgcggt cgaaggcgat
     3901 cgcgacgtcg ggccgcatcg gctttccacc cctatatgcg agcggaccgg agtacccgcc
     3961 gccggaggcc atcgtcggca tcccgggcgc gccggcgagc accgcgagct cggcggtcgc
     4021 gctcgcctcg acgtcgatcc gccggcgcgc cgatcgcctg ccgacgcgca cctcgacatc
     4081 gccgcgcgca cggacacgga cgcgcgtcgg cgcgaagccg ccgtccggga agctgacgtc
     4141 cgccgcggcg attcgcacgc cgttgcgagc gcccgcccgc agcgccaccg atcgcgctcg
     4201 tgcgaggtac tcgtcggtcg agagatggct cgggttcggc aggccgttcg gcaggacggc
     4261 cggctcgaag agtcggaagt agtcgtcgct catgcgatgc gccgcggaga ccgcggctag
     4321 gtcggcggca cgctgggggc gagacttgcc gcccagcgcc tggccaagag ctacgaggac
     4381 cagcgcgccg acgagcacgg cggcgaccac gccgagcatc aggacgagcg cctgaccgct
     4441 ctcgcgccga acggccatgg tcacggagcg accgccagca ggtcggcgag cttcgccacg
     4501 gccttcccga gctcgccgcg cgcctcgcgc ccggcgagcg cgagctgacc gcccatgcgg
     4561 gcctcgggca ggcgcaacgc acagcggccg tcccagcgtt cctcgccgcg atcgcgattg
     4621 aggacgacga ccggctcggg gccgacgcgg ctcaaggaat cggacaggac agccgcgagc
     4681 gcgggctcga ccgctgggga gccgacgagc accaccgtgt cgaccagcga ggccgcggct
     4741 gccgcgtcgc gcgagtcggt cacgtcgagt acgagcggcg ccaggtcgcg ggcggcatcg
     4801 acgaggccgg ccacgtcggg gcccgccgcg gcgaggcaga gtcgcccgac ggctcgcgtc
     4861 tgcgacggca gcgaccgctg cacggctcgc cccaaccgtc cggccgccgg tgtcccgagc
     4921 gggatcccgc cgccggcgat cgcgtccgcg ctgacgatcg ccgcgccctc gctgtcacgt
     4981 agcgcgagct cggccgcgag agcgcgtgcg acggtcgtgg tgccgcagcg gctcgccagg
     5041 ccggtgacgg cgaccgtcgg ccgtggagcg tccgtccagt gtcgctctgc gcgcgcgtcg
     5101 accggcgtgc gcggctcgat cggctcgacg aggaactcgg cgaccgcgct caacagcccg
     5161 cggcggcgcg ccgcaactag atcgctctgc tctcgcgcac tcgccgtcac ggcaactcga
     5221 ccgtggcgtc ggcatcgact tcgagcttgc gcgcgacgag cgccagcgga gccggcgggc
     5281 gcagcagcac gcggacggtc ccgccgctga cctccacccg ggcccgcgcc tccgaccagc
     5341 cgggcagcga gcgcttgacg gcggcgcccg cgtcgtcgcc acccgcgagc gccagcgcgc
     5401 cggcctcagc cgccactccg gcgagcaccg cggagtagcc gacggccagc agctgcagca
     5461 tgacgagccc gatcgcgagc atcgccggca ggccggcgac gacctcgacg agcgcttgcc
     5521 cgcgctcttc gccgagcagc ccggtcatcg cctctccccg agtgaggcgg tcgccccgac
     5581 cgccaccggc agccgccacc ggtgcagcag cagcggaaga tcgacgctca ccctcacgag
     5641 gccgtcgtcg cgctcgacgc cgagaccgtg ctcgagcccg tccggcagcg ccgcccgaac
     5701 ggcctcgcgt acgtcgccgt cgacgatgtc ggcgcgggcg ccggctcggg ccgcgtgcgc
     5761 cgccagccag gcggtgtggc cggcgagcgc gcactgccag acgaccgcgc ccaccagcag
     5821 gacgaacggc agtgccgcga ccagctcgac gctcgcctgt ccccgctctg accgccgcag
     5881 ccggcgcgca atacccatgc ccgccaccgt cgccgtcgcc ccgcgacggg acaacgcagt
     5941 tcggttacgg cagtgctaca gcggcgtcac cgaacgccgc caacggccag cagccacagc
     6001 accccggggg tgcggggggg
//
LOCUS       DATABV010000138        19343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1891299, whole genome shotgun sequence.
ACCESSION   DATABV010000138 DATABV010000000
VERSION     DATABV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 19343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19343
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891299"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VHF88_05855"
     CDS             <1..413
                     /locus_tag="VHF88_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161330.1"
                     /translation="VTGDDAGDRSLAATPATITAKEYGFRTSGIVPGRNRIAFRNTGS
                     EFHQVVAFPIPRGRSFAAGRQAILDAHAETAWVPVDVPHERATTAIEGGGELVAELTF
                     ARGRHLLLCFVSDRAGGAAQWTLGMSAQLKVGAP"
     gene            495..1658
                     /locus_tag="VHF88_05860"
     CDS             495..1658
                     /locus_tag="VHF88_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2161331.1"
                     /translation="MVDWSIARKVAGLTARSEDVIQDPGVDLVALSAEFAPRVAEYTR
                     LQPADPIPPPELVSRHGWSEANLDAMAELMKPVEQRMGERFEKAGPLAGPLRAGAGAA
                     LAAELGLVVGYMSQRVLGQYELSLIAGTANPRLLFVGANLRGAATALGVDREAFVRWV
                     TVHELTHAMQFGGVPWLRGHMGGLLKEYLETVDVKLDPTAGDGADETPDDDPAAKTSD
                     GPRGGRSGGLLGGALDKLRELDLPDPSDLADRFRDGGLAALVQNDEQRDVMDRMQAAM
                     ALVEGHAEHVMDALAPELVPQHEGLRAAMDARRQSRSAPERVLMRLLGMEMKLRQYKI
                     GKEFCDAVVADGGIEGLNRAWEGPEALPTLAELERPADWIARQAPDAGRLASG"
     gene            complement(1584..2264)
                     /locus_tag="VHF88_05865"
     CDS             complement(1584..2264)
                     /locus_tag="VHF88_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161332.1"
                     /translation="MAASRWAIALAVAVAAISASAVETGAASAAEVRVLDTSGPRPVP
                     CAALDLWNPQDPRGGCSTDMFSGSIDIVIRSVIGDMLFGHCTYEHDMLVDARGRTRLL
                     NIQSGGDNPCNDMGVCEYGRRRPWKGQIEAGPDGRLTHVIQACLDTCMGQFVGELRLS
                     LERIDGRWIQTAERALVGDSGYQIDGSWRMRNRDIDIRPAGGSAGGETAGVWRLTGDP
                     VGWPLQLG"
     gene            complement(2264..2755)
                     /locus_tag="VHF88_05870"
     CDS             complement(2264..2755)
                     /locus_tag="VHF88_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161333.1"
                     /translation="MSALRHIRRSYRLTLLLVVTCAGAGVLASCGGGSGADEDEIRTT
                     VRELQRAFVADQPGRVCGLLSRAARKHIRAMGHDLNPEASPPCDLDLYMFTEGIRKSP
                     TWRERSRRPIDDIEIDGDRATAAVAFEDGRTAGLPFVREDGRWRVDALFGAIPAGQQE
                     DNY"
     gene            2935..4212
                     /locus_tag="VHF88_05875"
     CDS             2935..4212
                     /locus_tag="VHF88_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161334.1"
                     /translation="MTTTPWGDATQLRARRLRPGPGADPAAVTRNQRERMYAAMVAAV
                     DANGYEGTRVADVVKLSGVSRSAFYKHFDDKLDCFLATLDEIVEMTGTALAKSYDESL
                     PGEQRLRTVADAFLDLVLEQPAAAQLGLVEVYAAGPEAVARLDRAVAVAEAAVARAFD
                     ESPRRKGMPRDVIRAIVGGVRKTVHTRVRRGEQTQLIEQLPQLLDWGLSYESPPERLR
                     RPRRRPDRGAAPLPDHSIPRERLVTAITDTIAERGYPETTIIEIADRASASLSTFYGN
                     FENKEEALLAALEREREQSLAAAGAAYDAAPDWPHGIHAGIDALFAFFAAEPAAASVA
                     IVDAFTAGPEGLEGGDQTIWAFHVFLAPGHELAPGTTPLVSEAVGNAVYSLVYGQIRA
                     ERVDRMRELTPVATFVALAPFVGAAEACAVANG"
     gene            4419..4994
                     /locus_tag="VHF88_05880"
     CDS             4419..4994
                     /locus_tag="VHF88_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161335.1"
                     /translation="MVHEVQRGRLLDALAEVISEEGYLDTTVHKILKRAGISRRTFYE
                     IFTDKEDCFLAAYQEAADHVLVLVQRACRLGGTPEIRIENSLRAMLEFAEREPQVARM
                     CIVEVMAAGEEARKRRAKTMERLTELVAGALEDRCETRDEAMLRARVLTGGVHEMVYD
                     SLCRGEVEGISDLAEEVVASHLSRPEAVHGG"
     gene            4984..5673
                     /locus_tag="VHF88_05885"
     CDS             4984..5673
                     /locus_tag="VHF88_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161336.1"
                     /translation="MAVSAPRAAPRRARRPDQLTGGRHGLSADQVRASQRARIVRAMT
                     EAVGEHGYHGVRVADVVWRAGVSRKTFYELFGGKDDCFAEAYGSWIDRLLATAFSAFE
                     TQVDWVDRLRAALTALLGALAREPDAARLCFSEGAAAGGATQQRRDQAARALCRLFDA
                     PGTPDGPLGETLRGGRVSELSETLRREIAAGRSERLPGLVPELMCAMVLPFLGMEAAQ
                     RELARGTASAG"
     gene            5670..6143
                     /locus_tag="VHF88_05890"
     CDS             5670..6143
                     /locus_tag="VHF88_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161337.1"
                     /translation="MSDGGAAPPDDTFADQAFAVAADAYARSTSDSWERAVHAAIARL
                     FGFLAERPLETRTCVVRDCGAGPRALGQRDRAIDRFAQLLQPGFAAAATPPPPVVAEA
                     ITGGIYELVRSHVLERRLGELPDAVPDATIVALAPFLGTAGAIDLVDSTKLQTGH"
     gene            6162..7463
                     /locus_tag="VHF88_05895"
     CDS             6162..7463
                     /locus_tag="VHF88_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161338.1"
                     /translation="MEGSVLTPWGRSDQLRDRRLRPGPGKQRADVERNHRERLFGATV
                     AIVDEQGYEGMNVRAVAARAGVSRGTVYEHFGGGQGCFLATFEALMDDGLARVSRAYA
                     NSDGAWDVRLGAALDALFEAIVAQPAAARLCFLETHAAGSAGAELRERGATALEALVR
                     DALDQSPERADMPAEVICAIAGAIRTVVETRLRRRREHELPALAPQLLRWLCSYHRPV
                     PALPETRVQASTAPRFVASSHRDRLFVGMAKAIHAKGYTGMSVSDLAAAAGVSFTTFY
                     DNYDGKEAAFLAACDFGIEQAFAAVRHAWDRECAHGWPAQVQAGMRELLGFLAAEPEW
                     AHMAMVDIFAAGARARARRDRTIRLFTSLVEPGRELAPEVEPIVVEALGGAVYSLMYS
                     RICHAGAQKLPEILPAAVFLLLAPFVGNPAAATLAREPATE"
     gene            7494..9437
                     /locus_tag="VHF88_05900"
     CDS             7494..9437
                     /locus_tag="VHF88_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEX2161339.1"
                     /translation="MCWSRWTATAFCLLVAGSTGVACGGDEGAPTGPTVTQHVTRDFG
                     REVVSTDDRLPIDDGETVLELLRRDHDVETTEAGRGVVGIDGERPETSPDGVESTWVL
                     NVNGIEADTAPRDYPLHPDDVVQWDLRDWDILLDVRATVGAFPETFTRGSFGKRFPTT
                     VECASPTSEACRRVKTALERAGIAIDGSRPRGGAPPRGAVQRARVLVGPWRHWRERSW
                     PARIDRGTRYSGVFARFTRGGDRMRLLDPDTRTAETVGAGSGLIAAMRPTEADLLWMV
                     TGVDDEGVERAASALDDRALHGAFAAAVTDAGIQKLPLASEPKAEDETRSGAAAAYTA
                     TTAQARTTVTELARAFARGNLRALCARISESAKRQAGLMAHGKVESCPRDLRQAFAIV
                     DPDSTLRFAGRRSIGKVSAAGGRATVTLEVAGRPHVDVALVESDGRWLLDSFFGTPQE
                     LAERSAAAVREAAFPSGPGRPARVADAGGVACFPFFADQFPEIDGGCELQVSGRRVAV
                     TVATPFGDFDFGDCEFGYTALADDRGRTWITDVSFTSRVNNGCADLRECRAGGEEAPW
                     RGAIRAVGDDRLVHRIDACLDTCVGFYTGELIVRLTRRRGRWTAAVARSAGGTVGLSG
                     FGLDGRLDVESDRLRIRAATPSG"
     gene            complement(9447..9905)
                     /locus_tag="VHF88_05905"
     CDS             complement(9447..9905)
                     /locus_tag="VHF88_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161340.1"
                     /translation="MSRGTIRRDGGRLAVLLALLALSLGAAACGGSDDDEATAQKGHA
                     AGMTGDEREIHDLYAEVTDRFYAKDAAGVCGLMTDAARRDQGKKSKQTCEEWFKALWQ
                     SGTLGKNKPYIAKLAIRGGKAQARVKTKNSKLYPVEFTREDGRWKVSDGF"
     gene            complement(9941..10384)
                     /locus_tag="VHF88_05910"
     CDS             complement(9941..10384)
                     /locus_tag="VHF88_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161341.1"
                     /translation="MAVALTVAAAAGCGSDDDAATLAESTATAPVRTAQGADDREIRA
                     VFEQMRTALAVADERRACAVMADGMRRDFARMAGNAGGTCESGFASYLEGGDVVEDTD
                     PELLRIQTDGTQAIVSAKVSGSKRPQRAFFIRERDDWKVRTWLRD"
     gene            complement(10580..14122)
                     /locus_tag="VHF88_05915"
     CDS             complement(10580..14122)
                     /locus_tag="VHF88_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161342.1"
                     /translation="MTILDRIRISARDRTFDSRDDLTMRHSSKLELRATASRVAGLAV
                     TGLIASALAAGPAQAAFGDQFGIAPVNDGAGPAQEAPAYPSAHAFWAGACNLAGAPAP
                     GNPPTPIDGGTGGIPATIPAPNGSSSPMAQIPAPATPPHCIDFGQPSPATSDPWDVPP
                     SWRLAPATQAGAHADGSTTMWLNKVGASPDGTLDNIYVSLPPGFVGDPNAVPKCTAEQ
                     FAVRPIGCPAASQVGVLQLFMEGSITGQNMQNSRYMDLSPVFNLEPRRGNAAELGVAY
                     VSGQAGSQGAGVTTVRIVAKPRTNGDFGVTAFTGQIPAALPVYSQAITLWGTPWEPAN
                     DIWRAPDGFMANEELPGDDPCHEQVGHPSGSGGARLPLNGLVPSCQEPYDPQWGPIKP
                     FLSNETDCNQRPAVELAIDAFQNPGKFTSEGDPVADDPGWKRYLSPQPAVVGCESLGF
                     NPDIAFSPTTAAADGASGLQVDLSIPQQTLPRDGAGNVLQTPAPGAGSAAAANYADAA
                     TAYWESPAGRATAHLKDTVVTLPAGVSVNPSAATGLQACGDGQVGLRQLGNPPLFNNG
                     DPFNKDGGADGAECPDGSKLGTARVRTPLLEQDLTGEVVLGEPKSTDPQSGEMFRTFL
                     VLRNVERGLVAKIYGTATADPGTGQITARFLNNPELPFDRLWLDFKSGSKGLFGMPQR
                     CGSPGWAAAFTPWSAVGAATPVADRTDGGGFGVSQNCANAFAPGMNAAMDTQAARANG
                     TFTFQFGRPEGQQYLRGLTAQLPKGLLASVKDVPLCSNAAAGAGACPAGSKIGLVDAK
                     AGSGDPFVLEQKGEVFLTEGYKGGEYGLAVKIRPVAGPFRGGMELSPVIVRQALHVDR
                     RTAQVTAISDPFPLIHHGIPLRVREVTVLINRGRFMLNPSDCAAKQVGATLTSDQGAA
                     AGVASRFQASGCANLPFKPKLKLRLTGRKQVKTGKHPGIRATVTQKGIPEAGIDKAEV
                     RLPKTLALDVENAQALCEYEDGTKPDLERHCPKGSIVGRARAVSPLLNRPLVGNVYFV
                     KNVRRSSTGNLIRTLPMIIVALRGEISVNLVGESDAKRGKLVNTFEEVPDAPISRFNL
                     NIKGGKNGIIAVTRTRRGLINLCTRGRQVAEADMDGHNGRRHDFDVNMRKPCARRAKV
                     VCRTKKQKAGRACKAKAKRLAAKRRAAKRRNARG"
     gene            complement(14119..18849)
                     /locus_tag="VHF88_05920"
     CDS             complement(14119..18849)
                     /locus_tag="VHF88_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161343.1"
                     /translation="MRGRKADTTVAAPLTFEGSSASIFGSFPEPASAVSGRSRGVSRT
                     SVTRVGKAGAFAATMSLLVVAIVAALAAATPAQADKTVDRFIGGAAGSAEGQFTQPRG
                     VAINESTGDVYVAEGLNAVHRVSRYSREGEFELMWGRGVEDGSPVAQVCTAATTPCQA
                     GGTGTAAGEFSTPVGLAIDQTDGSVYVYDRGNARVQKFGADGDFILMLGRGVNQTTGG
                     DVCTATSGNTCKAGVAGTGAGQFGATTQNSVRGIAVHPISGDLFVSDITNRRIHQFTS
                     GGTFVRAWGWGVDTGAAAFEVCTTASACNAAAAAGTDNGRFGTNNPAHLAVDADGVVY
                     ASDSGLNHRVLRFDSDGFGDPSTALLAPILADDQSPPGPLLSGTGSSGTTGLAIDPAS
                     GNLLVVRHRPNGGETLIQEIDTATVSEVARHGVGSGLTTVQGLAVDGTRDRLVASYAA
                     TPPGHGFFILDEDGPSSPTVMLRPASDVEARSAMLHGTIDPNGFAEYVFEVSTDGGTW
                     EPVGGGTLVGVGDVDVTATANGLAPNTPYRVRLRTTSVIGGDANGPVPTLEVLSLEGN
                     LVTDAIPADVTTVTASSITDTGALLVGRVNPNGSETAYWFEYGETTSYGNRVPVPAAL
                     AGSGTTELLVSQQLGGLVPNRTYHYRLAADNGVAHPVDGPGPRYGADRTFTTGSSSPN
                     ADAFGDSRAYELVSPADKVGGQGVGSWYNAPVASGRSGHAAYAGERFAVKGSFGSTLL
                     GHGAAAYGNDYVFAERTAAGWRSHSPFTHAVLSGQAYRLIDLSSAADDLSKVTLKNNG
                     GITKFFPEMADWPESTFGSPLMIGDWQGRWEIFGPTDRDTQISSGTTQANVDDAVVSA
                     DGSHVVGTSGMRGLAGIGDPSHQLVSGRTVYAHDSSGGLSDSFPGAGVRTLVQACSDE
                     TMIPNRLASGKLGEQACPDLPGTDASLVSRLGAALTSDTSSWRSTSKNAVSRDGSRIF
                     FMSPDPLAVPATATNNCAATFFGPPPPFTHPANGANTSCPPQLYMRESQPDGSFETRW
                     ISRAEEGLFGQQDASLLGPAQFEGATPDGAKVLFRTNSPLTTDDPNGTGAPAPVGGVK
                     TGTPSGNSWDLYLYDVESDELTRISGGPSGQADCNSPVGGMGVAALRFLAEDGSRAYF
                     TCAAPLDGALESSNGTITAPVGTASTTSDVNLYLYDARRPQPARWRFVARLPRAVATG
                     LHGDLSECASTGVADGTALGEVRSLSFAAGLQSANCVRGTADGAFVTMWTDGRLTADD
                     PDDRSGDIYGYDAERDELTRITRPAADAVGGAYECVNATDSSADVDEPRHCHGDVGFN
                     GGSVKEALPLLGVATHPAAGDRRAFFQSRSRLVAEDTDSNYDVYEWRNGELSLISTGA
                     SAGEGAFYRGNDRSGTNVYFATMDALTWEDHDAVMDVYTARVGGGFPEPPRPVVCGVV
                     AGGCHGNGAAAVAVPPAASTAPARVGDAVPDARPRLTVASLGRKARRRAARRGVIALR
                     VRTTTAGRVRAVARGKVGGKRRTLARAARNARSAGRVVVRLRLNRAARRHLRTGRRLR
                     VAVRVDMAGARQRTATVLLRRPGR"
BASE COUNT         2763 a         6974 c         6729 g         2877 t
ORIGIN      
        1 tggtgaccgg cgacgacgcc ggcgaccgct cccttgcggc cacccccgcg acgatcacag
       61 cgaaggagta cggcttccgc accagcggca tcgtccccgg gcgcaaccgc atcgccttcc
      121 gcaacaccgg cagcgagttc caccaggtgg tcgcctttcc gatcccccgc ggcagatcct
      181 tcgcggcggg gcgccaggcg atcctcgacg cccacgccga aaccgcctgg gtcccggtcg
      241 acgtgcccca cgagcgcgcg accacggcga tcgagggcgg cggcgagctg gtcgccgagc
      301 tgaccttcgc ccgcggccgc cacctgctgc tctgcttcgt ctccgaccgc gcaggcggcg
      361 ccgcgcagtg gacgctcggc atgtccgccc agctgaaggt cggtgcgccg tgagcccacc
      421 gaccgcggcg ccatcggctt cggcatcggc gggcgggggt cccgcgagct gagccgccgg
      481 ctatggtcaa ccgaatggtc gactggtcga ttgcgaggaa ggtcgccggg ctgacggcgc
      541 ggtcggagga cgtcatccag gacccgggcg tcgacctcgt cgcgctgagc gccgagtttg
      601 cgccgcgcgt cgccgagtac acgcgcctgc agccggcgga tccgatcccg ccgccggagc
      661 tggtgagccg ccacggctgg tcggaggcga acctcgacgc gatggccgag ctgatgaagc
      721 cggtcgagca gcggatgggc gagcggttcg agaaggccgg cccgctggcg gggccgctgc
      781 gcgcgggcgc cggcgccgcg ctggccgccg agctcgggct cgtcgtgggc tacatgtcgc
      841 agcgcgtgct cggccagtac gagctgtcgc tgatcgccgg cacggccaac ccgcggctgc
      901 tgttcgtcgg cgccaacctg cgcggcgccg ccaccgccct gggcgtagac cgcgaggcgt
      961 tcgttcgctg ggtgacggtc cacgagctga cccacgcgat gcagttcggc ggcgtgccgt
     1021 ggctgcgcgg ccacatgggc gggctgctga aggagtacct cgagaccgtc gacgtgaagc
     1081 tcgacccgac cgccggcgac ggagcggacg agacgcccga cgacgacccc gccgccaaga
     1141 ccagcgacgg cccgcgcggc ggccgcagcg gcggcctgct cggcggcgcg ctcgacaagc
     1201 tgcgtgagct cgacctgccc gacccctccg acctcgccga ccgcttccgc gacggcggcc
     1261 tcgcggcgct cgtgcagaac gacgagcagc gcgacgtgat ggaccggatg caggccgcga
     1321 tggcgctcgt cgagggtcac gcggagcacg tcatggacgc gctcgcaccc gagctggtcc
     1381 cccagcacga gggcctgcgc gccgcgatgg acgcccgccg ccagagccgc tccgcgcccg
     1441 agcgggtcct gatgcggctg ctcggcatgg agatgaagct gcgccagtac aagatcggca
     1501 aggagttctg cgacgccgtc gtcgccgacg gcggcatcga gggcctcaac cgcgcgtggg
     1561 agggccccga ggcgctgccg acgctagccg agctggagcg gccagccgac tggatcgccc
     1621 gtcaggcgcc agacgccggc cgtctcgcct ccggctgacc cgcccgccgg gcggatgtcg
     1681 atgtcgcggt tgcgcattcg ccagctgccg tcgatctggt agccgctgtc gccgacgagc
     1741 gcgcgctcgg cagtctggat ccagcgcccg tcgatacgct cgagggagag cctgagctcg
     1801 ccgacgaact gacccatgca ggtgtcgagg catgcctgga tgacgtgggt caggcgaccg
     1861 tcggggcccg cctcgatctg gcccttccac ggtcggcggc gaccgtactc gcagacgccc
     1921 atgtcgttgc acgggttgtc tccgcccgac tgaatgttca aaagccgcgt gcggccgcgc
     1981 gcgtcgacca gcatgtcgtg ctcgtaggtg cagtgcccga acagcatgtc gccgatcacg
     2041 ctgcggatga cgatgtcgat gctgccgctg aacatgtcgg tcgagcagcc tccgcgcgga
     2101 tcctgcggat tccagagatc cagcgccgcg cacggcaccg gccggggccc gctcgtgtcg
     2161 agtacccgaa cctcggcggc gctcgcggcg cccgtctcga cggcggacgc cgagatcgcc
     2221 gcaaccgcaa ccgccaatgc gatggcccag cgcgacgccg ccatcagtag ttgtcctcct
     2281 gctggccagc cggaatcgct ccgaacagcg cgtcgaccct ccagcggccg tcctcgcgca
     2341 cgaacggcag accggctgta cgcccgtcct cgaacgccac ggccgcggtg gcgcggtcac
     2401 cgtcgatctc gatgtcgtcg atcggccgcc tcgagcgctc gcgccaggtc ggcgacttcc
     2461 gtattccctc ggtgaacatg tagaggtcca ggtcgcacgg cggggacgcc tcggggttca
     2521 ggtcatgtcc catcgccctg atgtgcttgc gcgccgccct gctcagcagc ccgcagacgc
     2581 gaccgggctg gtcggcgacg aaggcccgct gcagctcccg gaccgtcgtc ctgatctcgt
     2641 cctcgtcagc gccgctgccg cccccgcagg acgcgagcac gccggctcca gcgcacgtca
     2701 cgacgagcag cagcgtgagg cgatacgagc ggcgtatatg ccgtaatgcg gacacgtccc
     2761 cttccctcca tcagggcggc caccacggcc gcgcggccca gcctaggcga tccgcgagcg
     2821 cccggcaacc acccggcaat tacccctctt ccaaaatgcc gaatttggga acgccgtctt
     2881 acccgcgatc ccgctcctga cgcgggccgg cgcgaccact agagtcctcg cgcgatgacc
     2941 acgacgccat ggggcgatgc gacccagctg cgtgcgcggc gactgcgacc cgggccgggc
     3001 gcagacccgg cggccgtcac gcgcaaccaa cgcgagcgga tgtacgcggc gatggtggcc
     3061 gcggtggatg ccaacggcta cgagggcacg cgcgtcgccg acgtggtgaa gctgtccggc
     3121 gtctcgcgca gcgctttcta caagcacttc gacgacaagc tcgactgctt cctcgcgacg
     3181 ctcgacgaga tcgtcgagat gaccggcacg gcactcgcga agagctacga cgagtcgctg
     3241 ccgggcgagc agcgcctgcg caccgtcgcc gatgccttcc tcgacctggt gctcgagcag
     3301 ccggcggcgg cgcagctcgg cctcgtcgag gtctatgcgg ccggaccgga ggcggtcgcc
     3361 cggctcgacc gcgcggtcgc cgtggcggag gcggcggtcg cgcgtgcctt cgacgagtcg
     3421 ccgcggcgca agggcatgcc gcgcgacgtg atccgcgcga tcgtcggcgg cgtccgcaag
     3481 acggtccaca cgcgcgtccg tcgcggcgag cagacgcagc tgatcgagca gctgccgcaa
     3541 ctgctcgact ggggcctgag ctacgagagc ccgcccgagc ggctgcgtcg cccgcgccgg
     3601 cggccggacc gcggcgccgc cccgctgccc gaccacagca tcccccgcga gcgcctcgtg
     3661 acggcgatca ccgacacgat cgccgagcgc ggctaccccg agacgacgat catcgagatc
     3721 gcggatcggg cgtcggcctc gctgagcacg ttctacggca acttcgagaa caaggaggag
     3781 gcgctgctcg ccgcgctcga gcgcgaacgc gagcagtcgc tggccgccgc cggggcggcc
     3841 tacgacgccg cgcccgactg gccgcacggc atccacgcgg ggatcgacgc gctgttcgcc
     3901 ttcttcgccg ccgagcccgc cgccgccagc gtcgcgatcg tcgacgcctt caccgccggc
     3961 ccggaaggcc tcgagggcgg cgaccagacg atctgggcct tccacgtctt cctcgcgccc
     4021 gggcacgagc tggcgcccgg gacgacgccg ctcgtctccg aggcggtcgg caacgccgtc
     4081 tactcgctcg tctacggcca gatccgcgcg gagcgcgtcg accgcatgcg agagctcacg
     4141 ccggttgcga ccttcgtcgc gctcgcgccg ttcgtcggcg ccgccgaggc ctgcgccgtc
     4201 gccaacggct agggccttcg tctgaggccg agcggcctgg gccgctcggc tgcctctgcc
     4261 agatcctggc cgcccggcca agttcttcag tatttcgatg ttcctatcct gtaagatgct
     4321 gttcctaaca tcgggcggag aggcgaaacg gctggatgga gactcgcaag cggcccagta
     4381 tcaagctgac gggaggaccc cacggaatcc cacgtgcggt ggtccacgag gtacaacgcg
     4441 gacggctgct cgacgcgctc gccgaggtga tatccgagga gggctacctc gacaccacgg
     4501 tgcacaagat cctcaagcgc gcgggcatct cgcgccggac cttctacgag atcttcaccg
     4561 acaaggagga ctgcttcctc gccgcctacc aggaggcggc cgaccacgtg ctcgtgctcg
     4621 tccagcgcgc ctgccgcctc ggcggcacgc cggagatccg gatcgagaac agcctgcgcg
     4681 cgatgctcga gttcgccgag cgcgagccgc aggtcgcgcg gatgtgcatc gtcgaggtga
     4741 tggccgccgg cgaggaggcc cgcaagcgcc gcgccaagac gatggagcgg ctgaccgaac
     4801 tcgtcgccgg cgcgctcgag gaccgctgcg agacccgcga cgaggcgatg ctgcgcgctc
     4861 gggtgctgac cggcggcgtt cacgagatgg tctacgactc gctctgccgc ggcgaggtcg
     4921 aggggatctc cgacctcgcc gaggaggtcg tggcctccca cctgtcgcgg cccgaggccg
     4981 tacatggcgg ttagcgcacc gcgcgccgcg ccgcgccgcg cgcgccggcc ggaccagctg
     5041 accggcggcc gtcacggact gagcgccgac caggtgcgcg cctcccagcg cgcgcgcatc
     5101 gtccgggcga tgaccgaggc ggtcggcgag cacggctacc acggcgtccg ggtcgccgac
     5161 gtcgtctggc gcgccggcgt ctcgcgcaag accttctacg agctgttcgg cggcaaggac
     5221 gactgcttcg ccgaggccta cgggtcctgg atcgaccgcc tgctggcgac cgcgttctcc
     5281 gccttcgaga cgcaggtcga ctgggtcgac cggctgcgcg ccgccttgac ggcgctgctc
     5341 ggcgccctgg cgcgcgagcc ggacgcggcg cgtctgtgct tcagcgaggg cgcggcggcc
     5401 ggcggcgcca cccagcagcg gcgcgaccag gccgcccggg cgctctgccg cctgttcgac
     5461 gcccccggca cgcctgacgg gccgctcggc gagacgctcc gtggcggccg ggtcagcgag
     5521 ctgagcgaga cactccgtcg tgagatagcc gccggccgca gcgagcggct tcccggcctc
     5581 gtgcccgagc tgatgtgcgc gatggtcctg ccgttcctcg gcatggaggc cgcacagcgc
     5641 gagctggccc gcggcacagc atcggcggga tgagcgacgg gggcgcagcg cctcccgacg
     5701 acacgttcgc cgaccaggcg ttcgccgtgg ccgccgacgc ctacgctcgg tcgacctccg
     5761 acagctggga gcgcgccgtc cacgcggcga tcgcgcgcct gttcggcttc ctcgccgagc
     5821 ggccgctcga gacgcggacc tgcgtcgtca gggactgcgg cgccggtccg cgcgcgctcg
     5881 ggcagcgcga ccgcgcgatc gaccgcttcg cgcagctgct gcaaccgggc ttcgccgcgg
     5941 ccgcgacgcc gcctccgccg gtggtcgccg aggcgatcac cggcggcatc tacgagctgg
     6001 tccgcagcca tgtgctcgag cgccggctag gcgagctgcc ggatgccgtc cccgacgcca
     6061 ccatcgtcgc gctggcaccg ttcctgggga cggcaggagc gatcgacctg gtggattcga
     6121 caaagctgca aaccggccac tgacgagcga tactccaggc agtggaggga tcggtgctga
     6181 caccttgggg tcggtcagac cagttgcgcg accggcggct gcgccccggg ccgggcaagc
     6241 agcgcgcgga cgtcgagcgc aaccatcgcg agcggctgtt cggggctacg gtcgcaatcg
     6301 tcgatgagca gggctacgag ggcatgaacg tgcgtgccgt cgctgcccgc gccggcgtct
     6361 cgcgcggcac cgtctacgag cacttcggcg gtggccaggg ttgcttcctc gcgaccttcg
     6421 aggcgttgat ggacgatggc ctcgcgcgag tctcacgcgc ctacgcaaac agcgacgggg
     6481 cgtgggacgt caggctcgga gcggcgctcg acgcgttgtt cgaggcgatc gtcgcccagc
     6541 cggcagccgc ccgcctgtgc ttcctggaga cgcacgccgc gggatccgcc ggggccgagc
     6601 tgcgcgagcg cggcgccacc gcgctcgagg cgctggtgcg cgacgcgctc gaccagtcgc
     6661 ccgagcgcgc cgacatgccc gccgaggtga tctgcgcgat cgccggggcg attcgcaccg
     6721 tcgtcgagac gcggctgcgc cgccggcgcg agcacgagct gcccgcgctg gcgccgcagc
     6781 tgctgcgctg gctctgcagc tatcaccggc ccgtcccggc gctcccggag acccgagtcc
     6841 aagcctcgac ggcgccgcgc ttcgtcgcca gctcgcaccg cgaccggctc ttcgtcggca
     6901 tggccaaggc gatccacgcc aagggctaca ccgggatgag cgtctccgac ctcgccgccg
     6961 ccgccggcgt gtcgttcacc accttctacg acaactacga cggcaaggag gcggcgttcc
     7021 tcgcggcgtg cgacttcggc atcgagcagg cgttcgccgc cgtgcgccac gcctgggacc
     7081 gagagtgcgc gcatggctgg cccgcgcagg tccaggccgg gatgcgcgag ctgctcggct
     7141 tcctggccgc cgagccggag tgggcacaca tggcgatggt cgacatcttc gccgcgggcg
     7201 cgcgcgcgcg agcccgccgc gaccggacga tccgcctgtt caccagcctc gtcgagccgg
     7261 ggcgcgagct tgccccagag gtcgagccga tcgtcgtcga ggcgctcggc ggggcggtct
     7321 actcgctgat gtacagccgg atctgccacg ccggagcgca gaagctgcct gagatcctgc
     7381 ccgccgccgt gttcctgctg ctcgcgccgt tcgtggggaa tcccgccgcg gcgactcttg
     7441 cgagggagcc ggcgaccgaa taggctcctc ccggtcggag attggaggga ccgatgtgct
     7501 ggagccgatg gacggcgacg gcgttctgct tgctcgtggc tgggtcgacg ggcgtcgcct
     7561 gcggtggcga cgagggcgct cccacaggcc ccacggtgac acagcacgtg acgcgcgact
     7621 tcgggcgcga ggtcgtgtcg acggacgatc gcctgccgat cgacgacggg gagaccgtgc
     7681 tcgagctgct gcgccgcgac cacgacgtcg agacgaccga ggccgggcgt ggcgtcgtcg
     7741 gcatcgacgg cgagcgcccc gagacgtccc ccgacggggt cgagtcgacg tgggtgctga
     7801 acgtcaacgg catcgaggcg gacaccgctc cacgcgacta cccgctccac cccgacgacg
     7861 tggtgcagtg ggacctgcgc gactgggaca tcctgctcga cgtccgcgcg accgtcggcg
     7921 ccttcccgga gacgttcacg cgcggcagct tcggcaagcg cttcccgacc acggtggagt
     7981 gcgcgagccc gacgtccgag gcctgccggc gggtcaagac ggcgctcgag cgggccggca
     8041 tcgccatcga tggctcgcgg ccgcgcggcg gtgccccgcc gcgcggcgcg gtgcagcggg
     8101 cgcgcgtgct ggtcggccct tggcggcact ggcgggagcg ctcctggccg gcgcggatag
     8161 accgggggac gcgctacagc ggcgtgttcg cgcgcttcac ccgcggcggg gaccgcatgc
     8221 gcctgctcga ccccgacacc cgaaccgccg agacggtggg cgccggtagt ggcctcatcg
     8281 ccgcgatgcg cccgacggaa gcggacctcc tctggatggt gaccggcgtc gacgacgagg
     8341 gtgtcgagcg cgccgcttcc gccctcgacg accgcgcgct gcacggcgcg ttcgcggccg
     8401 cggtgaccga cgcgggcatc cagaagctgc cactggcgag cgagccgaag gcggaggacg
     8461 agacccgctc cggcgccgcc gccgcataca ccgcgacaac ggcccaggcg aggacgaccg
     8521 tcactgagct cgcccgcgcc ttcgcgcgcg ggaacctgcg ggcgctgtgc gcccggataa
     8581 gcgagagcgc gaagcgacag gccgggctga tggcccacgg caaggtcgag agctgtccgc
     8641 gggacctgcg ccaggcgttc gcgatcgtcg acccggattc caccctccgc ttcgccggca
     8701 ggcgctcgat cgggaaggtg agcgccgcgg gcggtcgcgc gacggtgacg ctcgaggtcg
     8761 ccggccggcc gcacgtcgac gtcgcgctgg tcgagagcga cggccggtgg ctgctcgaca
     8821 gcttcttcgg gaccccgcaa gagctcgccg agcggagcgc cgccgcggtc cgcgaggcgg
     8881 cgttcccgag cgggccgggc cgcccggcgc gcgtcgccga cgcgggcggc gtcgcctgct
     8941 tcccgttctt cgcggaccag ttccccgaga tcgacggcgg ctgcgagctg caggtcagcg
     9001 gacggcgcgt cgcggtgacc gtcgcgaccc cgttcggcga cttcgacttc ggcgactgcg
     9061 agttcggcta caccgccctg gcggacgacc gcggccgcac ctggataacc gacgtgtcgt
     9121 tcacctcgcg cgtcaacaac ggctgcgccg acctgaggga gtgcagggcc ggcggcgagg
     9181 aggcgccctg gaggggggcc atccgcgccg tcggcgacga ccggctcgtc caccgcatcg
     9241 acgcctgcct ggacacctgc gtcggcttct acacgggtga gctgatcgtg cgcctcacgc
     9301 gccggcgcgg ccgctggaca gccgcggtcg cgcggtcagc gggcggaacg gtcggtctga
     9361 gcgggttcgg gctggacggc cggctcgacg tcgagtccga ccgcctgcgg atccgtgccg
     9421 ccacgccctc gggctgagcc cggcggtcag aacccgtcgc tgaccttcca gcgtccgtcc
     9481 tcgcgcgtga actccaccgg gtagagcttg ctgttcttgg tcttgacacg agcctgcgcc
     9541 ttgccgccgc ggatcgccag cttcgcgatg tagggcttgt tcttcccgag cgttccgctc
     9601 tgccacagcg ccttgaacca ctcctcgcag gtctgcttgc tcttcttgcc ctggtcgcgc
     9661 cgcgcggcgt cggtcatcag gccgcagacg cccgcggcat ccttggcgta gaagcggtcg
     9721 gtgacctcgg cgtagaggtc gtggatctcg cgctcgtcgc cggtcatgcc cgcggcgtgg
     9781 cccttctgag cagtcgcctc gtcgtcgtcc gacccgccgc acgccgcggc tcccagcgac
     9841 agggccagca gcgccagcag taccgcgagg cgtccgccgt cacggcgaat ggtccctctg
     9901 ctcatgttcc ctccagtcgt gtccgcctcg atgcgagacg tcagtccctc agccacgtcc
     9961 gcaccttcca gtcgtctcgc tcccggatga agaaggcccg ttgcgggcgc ttgctccccg
    10021 agactttggc gctcacgatc gcctgggtgc cgtcggtctg tatgcgcagg agctcgggat
    10081 cggtgtcctc gacgacgtcg cccccctcca ggtaggacgc aaagccgctc tcgcaggtcc
    10141 ctccagcgtt ccccgccatc cgggcaaagt cgcgacgcat cccgtcggcc atcacggcgc
    10201 aggcgcgccg ctcgtcggct accgccagcg cggtccgcat ctgctcgaac acggcgcgga
    10261 tctcgcgatc gtcggcgccc tgggccgtcc gcacgggggc cgtcgcggtg ctctctgcca
    10321 gcgtggccgc gtcgtcgtcc gatccgcagc ccgcggctgc cgcgacggtg agcgccactg
    10381 ccaacagagc tactgctcgc catccagcgc cgtcaatgcg tcgacccgtc aacggtccct
    10441 ccattccccg gttgcgacgc gccacgcgag tcacgaaagc ctccccgccg gcacccctcc
    10501 aattgcctca accctatccg accgcgcacc tgcccgcaag cggcctcccg ggcgggcagc
    10561 gcccacccgg gagggcccgc tagccgcgcg cgttcctgcg cttggccgct ctgcgcttgg
    10621 cggcgaggcg cttcgccttc gccttgcacg ccctgccggc cttctgcttc ttggtcctgc
    10681 acacgacctt cgccctgcgc gcgcacggct tgcgcatgtt gacgtcgaag tcgtgacggc
    10741 ggccgttgtg gccgtccatg tcggcctcgg cgacctggcg cccgcgggtg cacaggttga
    10801 tcaatcccct gcgggtgcgg gtgacggcga tgatgccgtt cttgccgccc ttgatgttga
    10861 ggttgaaccg gctgatcggc gcgtcgggca cctcctcgaa ggtgttgacc agcttgcccc
    10921 gcttcgcgtc ggactcgccg acgaggttca ccgagatctc gccgcgcagg gcgacgatga
    10981 tcatcggcag ggtgcggatc aggttgcccg tcgacgaacg ccggacgttc ttgacgaagt
    11041 acacgttccc gacgagcggc cggttgagca gcgggctcac cgcacgcgcc cgtccgacga
    11101 tgctgccctt cgggcagtgg cgctcgaggt ccggcttggt cccgtcctcg tactcgcaga
    11161 gcgcctgcgc gttctccacg tcgagcgcca gtgtcttggg cagccgtacc tcggccttgt
    11221 cgattcccgc ctcggggatc cccttctggg tcaccgtcgc gcggatgccg gggtgcttgc
    11281 cggtcttgac ctgcttcctg cccgtgagcc gcagcttgag cttcggcttg aacggcaggt
    11341 tcgcgcagcc ggaggcctgg aaccggctcg ccacgccagc ggccgcgccc tggtccgagg
    11401 tcagcgtcgc gccgacctgc ttggccgcgc agtcggacgg gttcagcatg aaccggccgc
    11461 ggttgatcag caccgtcacc tcgcgcaccc gcaacgggat gccgtggtgg atcagcggga
    11521 acggatcgct gatggccgtc acctgggccg tccggcgatc cacgtggagc gcctggcgca
    11581 cgatcaccgg cgacagctcc atgccgcccc ggaacggtcc ggcgaccggc cggatcttga
    11641 ccgcgaggcc gtactctccc cccttgtagc cctcggtgag gaacacctcg cccttctgct
    11701 cgagcacgaa cgggtcgccc gagcccgcct ttgcgtccac caggccgatc ttcgagccag
    11761 cgggacaggc gcccgcgccg gcggccgcgt tcgagcagag cggcacgtcc ttcacggagg
    11821 ccagcaggcc cttgggcagc tgggcggtca ggccgcgtag gtactgctgg ccctcgggac
    11881 gcccgaactg gaacgtgaac gtcccgttgg cacgcgcggc ctgcgtgtcc atcgccgcgt
    11941 tcatgccggg cgcgaacgcg ttggcgcagt tctggctgac gccgaagccg ccgccgtcgg
    12001 tgcggtcggc caccggagtt gccgccccca ccgcggacca cggcgtgaac gccgcggccc
    12061 agccggggct gccgcagcgt tgcggcatcc cgaacagtcc cttcgacccg ctcttgaagt
    12121 ccagccacag ccggtcgaac ggcagctcgg ggttgttgag gaaccgtgcc gtgatctggc
    12181 cggtgccggg gtcggccgtc gcggtgccgt aaatcttggc gaccaggccc cgctcgacgt
    12241 tgcgcagcac gaggaaggtg cggaacatct cgcccgactg cgggtcggtc gacttcggct
    12301 cgccaagcac cacctcgccg gtcagatcct gctcgagcag cggcgtgcgg acccgcgccg
    12361 tccctagctt cgagccgtcc gggcactccg ccccgtctgc gccgccgtcc ttgttgaacg
    12421 gatcgccgtt gttgaacagc ggcgggttgc ccagctggcg cagcccgacc tggccgtcgc
    12481 cgcaagcctg caggccggtg gcggcggacg ggttgaccga cacgcccgcc ggcaaggtca
    12541 cgacggtgtc cttgaggtgg gcggtcgcgc gtccggcggg cgactcccag taggcggtgg
    12601 ccgcgtcggc gtagttggcc gcggcggccg agccggcgcc tggggccggc gtctggagca
    12661 cgttgccggc gccgtcacgc ggcaaggtct gctgcgggat cgagaggtcc acctgcaggc
    12721 cagaagcccc gtcggccgcc gccgtggtcg gcgagaacgc gatgtcggga ttgaagccga
    12781 ggctctcgca gccgaccacc gccggctgcg gcgacaggta ccgcttccac cccgggtcgt
    12841 ccgccacggg atcgccctcg gacgtgaact tgcccgggtt ctggaacgcg tcgatggcca
    12901 gctctacggc tgggcgctgg ttgcagtcgg tctcgttcga caggaacggc ttgatcggtc
    12961 cccactgcgg gtcgtagggc tcctgacagc tggggacgag gccgttgagc ggtagacggg
    13021 ctccccctga acccgacgga tgcccgacct gctcgtgaca gggatcgtct ccgggaagct
    13081 cctcgtttgc catgaaaccg tccggcgccc gccagatgtc gttggcaggc tcccatgggg
    13141 ttccccagag ggtgatcgcc tgcgagtaga ccggcagagc cgcggggatc tggccggtga
    13201 acgcggtcac cccgaagtcg ccgttggtcc gcggcttggc gacgatccgt acggtcgtca
    13261 cgccggctcc ttgcgatccc gcctgaccgg agacgtaggc gacccctagc tccgcagcgt
    13321 tgcccctgcg aggctcgagg ttgaagaccg gggacaagtc catgtacctg ctgttctgca
    13381 tgttctggcc cgtgatcgag ccctccatga agagctggag cacccctacc tggctcgcag
    13441 cggggcagcc gatcggccgg acggcgaact gctcggcggt gcacttcggc accgcgttgg
    13501 gatcgccgac gaagcctggc ggcaggctga cgtagatgtt gtcgagcgtc ccgtcggggc
    13561 tggcgccgac cttgttcagc cacatcgtcg tgcttccgtc ggcatgagcg cccgcctgcg
    13621 tcgccggagc aagccgccag ctcggcggca cgtcccaggg atcgctcgtc gccgggctgg
    13681 gctgcccgaa gtcgatgcag tgcggcggcg tcgccggcgc cgggatctgc gccatcgggc
    13741 tggacgatcc gttcggagcg ggaatcgtgg ctggaatgcc gccggtcccg ccgtcgatcg
    13801 gcgtcggcgg gttacctggc gcgggggcgc cggcaaggtt gcatgcgccc gcccagaagg
    13861 catgggcgct cgggtaggcg ggagcctcct gcgccggacc ggcgccgtcg ttgaccggcg
    13921 cgataccgaa ctggtcgccg aacgccgcct gcgccggacc ggccgccaac gccgaggcga
    13981 tcagccccgt cacggccagc ccggcgaccc tggaggcggt cgcgcggagc tcgagcttgc
    14041 tgctgtgtcg catcgtcagg tcgtcccttg agtcgaaggt gcggtcacgg gcgcttatgc
    14101 ggattcggtc gagaatcgtc atcggcccgg cctccgcagc aggacggtgg ccgttcgctg
    14161 ccgagccccg gccatgtcga cccgcaccgc gacacgtagc cgccgtccgg ttcgcagatg
    14221 gcgccgtgcc gcccggttga gccgcagacg aacgacgacc cgtccagcgg agcgcgcgtt
    14281 gcgggccgcc cgtgccaggg tcctgcgctt ccctccgacc ttgccacgcg cgacggctct
    14341 cacccggccg gccgtcgtcg tgcgcacgcg caaggcgatc acgcccctac gagccgcgcg
    14401 ccggcgcgcc ttgcggccga ggctggcgac cgtgagccgc ggacgcgcgt cgggcactgc
    14461 gtcgcccacg cgcgccggcg ccgtcgacgc tgcgggtggt accgcgacgg cagccgcgcc
    14521 gttcccgtga cagcctcctg ccacgacccc gcacaccaca gggcggggcg gctccgggaa
    14581 tcctccaccg acgcgcgcgg tgtagacatc catcaccgca tcgtggtcct cccacgtcag
    14641 ggcgtccatg gtcgcgaagt agacgttcgt gccgctgcgg tcgttgccgc ggtagaaggc
    14701 cccctccccg gcgctggcgc cggtgctgat caacgagagc tcgccgttgc gccactcgta
    14761 tacgtcatag ttgctatcgg tgtcctcggc gaccagccgg cttctgctct ggaagaacgc
    14821 acgccgatcg cccgcggctg ggtgggtcgc gaccccgagc agcggcaacg cctccttgac
    14881 gctgccgccg ttgaacccga cgtcaccgtg gcagtgacgc ggctcatcga cgtcggcact
    14941 ggagtcggtg gcattcacgc actcgtacgc gcccccgacg gcatccgccg ccggacgcgt
    15001 gatgcgcgtg agctcgtcgc gctccgcgtc gtagccgtag atgtcccctg acctgtcgtc
    15061 gggatcgtcg gccgtcaacc gaccgtcggt ccacatggtg acaaaagcgc cgtcggccgt
    15121 gccgcgtacg cagttcgcgg actgaagacc ggctgcgaag ctcagggatc tcacctcacc
    15181 aagagccgtg ccgtcggcca cgccagtgct cgcacactcc gacaggtcgc cgtgcaggcc
    15241 ggtggcgacg gcgcgcggca gtcgcgccac gaacctccac cgcgctggct gcggacggcg
    15301 tgcgtcgtag agatagaggt tcacatcgct ggtcgtcgac gccgtgccca cgggagccgt
    15361 gatcgtgccg ttgctggact cgagcgcgcc gtcgagcggc gccgcgcagg tgaagtatgc
    15421 cctcgaccca tcctcggcga ggaaccgcaa ggcggcgacg cccataccac caaccgggct
    15481 attgcagtcc gcctggccgc tagggccacc ggagatccgg gtgagctcgt ccgactcgac
    15541 gtcgtacagg tagagatccc acgagttgcc ggacggcgtc ccggtcttca caccgcccac
    15601 cggcgccggg gcaccggtgc cgttggggtc atcggtcgtc agtggcgagt tggtccggaa
    15661 cagaaccttg gcgccatctg gcgtcgcccc ctcgaactgt gcggggccga gcagcgaagc
    15721 gtcctgctgg ccgaatagtc cctcctcggc acgcgagatc cagcgcgtct cgaagctgcc
    15781 gtccggctga ctctcccgca tgtagagctg tggcgggcag ctcgtgttgg cgccgttcgc
    15841 aggatgggtg aacggaggcg gggggccgaa gaacgtggct gcacagttgt tcgtggctgt
    15901 tgccggcacc gccaatgggt cggggctcat gaagaatatt cgcgagccat cccgggagac
    15961 agcgttctta gacgtcgagc gccaacttga cgtatcgctg gttagagcgg cgcctagtct
    16021 cgagacgagc gacgcgtcgg ttccgggcag atccgggcat gcctgctcgc cgagcttgcc
    16081 agacgcgagc cggttgggga tcatggtctc atcgctacag gcttggacga gagtgcgtac
    16141 tcccgccccg gggaacgagt ccgagagccc cccggagctg tcatgcgcat agacagtgcg
    16201 cccgctgacg agctggtgcg agggatcgcc gatgccggcc agcccgcgca taccggacgt
    16261 cccgacaaca tgcgagccat cagcagagac gacagcgtcg tccacgttgg cttgcgttgt
    16321 gccggatgag atctgcgtgt cacggtcggt aggaccgaag atctcccaac gcccctgcca
    16381 gtcgccgatc atcaacggac tgccgaacgt gctctcaggc caatccgcca tctcaggaaa
    16441 aaacttggtg attccgccgt tgtttttcag cgtgaccttc gacagatcat cagccgcact
    16501 ggataggtcg atgaggcgat aggcctgccc gctgagcacc gcgtgcgtga acggagagtg
    16561 gctgcgccat cccgcggccg tccgctcggc gaacacgtag tcgtttccat aagcggcggc
    16621 accgtgtccg aggagcgtcg acccgaagct tcccttgacc gcgaagcgct cgcccgcata
    16681 ggcggcgtgg cccgaccggc cagatgctac gggggcgttg taccagctgc ccacaccctg
    16741 cccgccgacc ttgtccgcag gagagacgag ctcatacgcg cgactgtcac cgaaggcgtc
    16801 cgcgttcggt gatgaagacc ctgtcgtgaa cgtccggtcg gcgccatagc gcggaccggg
    16861 gccatcgacg ggatgggcga cgccgttgtc cgccgccagt cggtagtgat acgtccgatt
    16921 tggaaccaag ccgcccagct gctgagagac cagcagctcc gtcgtcccgg atccggccag
    16981 agccgccggc accggaaccc tgtttccgta tgacgtggtc tcgccatact cgaaccagta
    17041 cgccgtctcc gagccgttcg gattgacccg gccgaccagc agggcgcccg tgtcggtgat
    17101 gctggacgcg gtgacggtcg tgacgtcagc agggatcgca tctgtcacca agttgccctc
    17161 cagcgacagg acctcgaggg tgggcacagg gccgttggcg tcgccgccaa tcaccgacgt
    17221 ggttcggagg cgtacccggt agggcgtgtt gggcgcaaga ccatttgctg tggccgtgac
    17281 atcgacatcc cccaccccga cgagcgttcc gccgccgacg ggctcccagg tgccgccgtc
    17341 ggtgctgacc tcgaacacgt actcggcgaa gccgttcggg tcgatcgtgc cgtgcagcat
    17401 cgcgctgcgc gcctcgacgt ccgatgcggg acgaagcatc accgtcggtg agctggggcc
    17461 gtcctcgtcg aggatgaaga acccgtggcc cggaggggtg gccgcgtagg aggcgacaag
    17521 cctgtcacgc gttccgtcga cggcgaggcc ttggacggtc gtcaggccac tgccgacacc
    17581 gtggcgggcg acctcgctga cggtcgccgt gtcgatctcc tggatcagcg tctcaccgcc
    17641 gttcggacgg tgacggacca ccagcaggtt tccgctggcc ggatctatcg ccaggccggt
    17701 cgtgccactg ctacccgtac cagaaaggag cggaccgggg ggcgactgat cgtctgcgag
    17761 gatcggcgca agcagcgcgg tcgaagggtc gccgaagccg tccgagtcga acctcaggac
    17821 tctatggttg aggcccgaat cgctggcata gacgactccg tcggcatcga cggcgagatg
    17881 cgctggattg ttcgtaccaa agcggccgtt gtccgtaccc gccgctgcgg cggcgttgca
    17941 ggcgctggcc gtggtgcaca cctcgaacgc agcggcgccg gtgtcgacgc cccatcccca
    18001 agcccgtacg aacgtgccgc cggaagtgaa ctggtggatg cgtcggttgg ttatgtcgct
    18061 aacgaacaaa tcaccgctta tgggatgcac ggcgatgcca cgcacactgt tctgggtcgt
    18121 ggcgccgaac tgacccgcgc ccgtgccggc aacgcctgcc ttgcaggtgt tgccagaggt
    18181 agcggtgcag acgtccccgc ctgtcgtctg gttcacgcca cggccgagca tcaagatgaa
    18241 gtcgccgtcc gcgccaaact tctgcacgcg agcattgcca cggtcgtaca catacaccga
    18301 tccgtccgtc tggtcgatcg cgagcccgac cggcgtgctg aactcaccag cagccgtccc
    18361 cgtgccgcca gcttgacaag gtgtggtagc ggcggtgcaa acctgggcga ccggagagcc
    18421 gtcctcgacg cctcggcccc acatcagctc gaactccccc tcgcggctat agcgcgagac
    18481 gcgatggacg gcgttcaatc cctccgccac gtagacgtcg ccggtcgact cgttgatcgc
    18541 gaccccccga ggctgggtga actgcccctc cgcagagccg gcagctccac cgatgaatcg
    18601 gtccacggtc ttgtcggcct gggctggggt cgctgccgca agagccgcca cgatcgcgac
    18661 caccagaaga gacatagtcg ctgcaaaagc tcccgctttc ccaacgcggg tcacgctcgt
    18721 gcggctgacg cccctcgagc gccccgagac tgcgctcgct ggctcgggga aagacccgaa
    18781 aatggacgca ctactccctt caaaggtgag cggagcagca acggtggtgt ccgccttgcg
    18841 accacgcacg gcagaaagga accttccgat tcctctcgtg acacccaggc tgcttccagc
    18901 tcgcctcatc taactacttc ctctcctcgt cgctaactcc ggcccggatc tgacgatcca
    18961 cgccggcttg atcgaagttc tgccgccacc gagacgcaca caagcgtccc gacaaacgtc
    19021 tctaagtcct gcgcaatgcc gcaacggccg ctaacaaacg ccgattcccc caatgaaggc
    19081 gagacggagc tagcagtcga tacggggact gaggggtggg tggggggttc actcgtatgg
    19141 gtgcagccga ccgacaggcc agctgacctc gagatcgtcg ccacactgcc caccgctctc
    19201 gagccctcga ggtcctgctg acacgcatcc atatgagaag ccacgtagga gcgcctatgg
    19261 agctccgtcg tgggatctac gggatccggc ccagaaggtg ctgagggatc gacgggtgtg
    19321 aaagagagga tcccggccga ggt
//
LOCUS       DATABV010000139        24155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_766452, whole genome shotgun sequence.
ACCESSION   DATABV010000139 DATABV010000000
VERSION     DATABV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24155
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766452"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VHF88_05925"
     CDS             <1..1395
                     /locus_tag="VHF88_05925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2161344.1"
                     /translation="QTGTGKTAAFGLPLLDYLDPSDPAVQALVLTPTRELCIQVTQAL
                     RAYGAQHGIEVVAVFGGAPIRDQASRLKQEAQVVVGTVGRVMDMIRRHYLFLDQARYV
                     VLDEADEMLDLGFFEDVDEILARCPSGRQTALFSATMPPPIKKLAEKRMFDPVTIKVK
                     AAQLTIDTVAQFHIEVPDKDKAERLGEVLKAERPEQAIIFARTKIGVDRLARTLDRQG
                     LRVKTLHGNMSQGARDGVMIAFKGGRERLLVATDVAARGLDITGVSHVVNYDVPNSPD
                     IYVHRIGRTGRAGETGRAITLITPKQREDLEAIERHANTEIPAWTAPNGAAPERPKRV
                     RPPKAAKPEAEAPKVAAVEQQDEEPPEAPQPEPRERPQPRRPRHTKPRPRDGKVAKLL
                     IGGGRAQGFQPADVVAAIVDNCHLDGEDVRKVRVLERFSFVEVPAERAGEVVEKVNGN
                     EVRGVPLRLEVAKR"
     gene            1392..1859
                     /locus_tag="VHF88_05930"
     CDS             1392..1859
                     /locus_tag="VHF88_05930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2161345.1"
                     /translation="MSQATLHTTHGPIEVQFFDDDAPKTVENFRKLSADGFYDGLSFH
                     RVIKDFMIQGGCPQGTGTGGPGYTFEDEFNDHKVVRGALAMANAGPNTNGSQFFIVTT
                     AEASWLDGKHTVFGEVVSGMDAVDAIEAVPTGPGDQPEEPVVIERVELADGAG"
     gene            complement(1879..2274)
                     /locus_tag="VHF88_05935"
     CDS             complement(1879..2274)
                     /locus_tag="VHF88_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2161346.1"
                     /translation="MDTNVLLDLLGGDPEFGPASREAIRGARQEGKLIACDIVWAETG
                     ASFESAGAARAALDSLGLEYSPVDARAALDAAQAWRAYRRAGGTRDRVIADFLVAAHA
                     GIHADRLLTRDRGFYRTRFEGLTILEPTR"
     gene            complement(2283..2513)
                     /locus_tag="VHF88_05940"
     CDS             complement(2283..2513)
                     /locus_tag="VHF88_05940"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX2161347.1"
                     /translation="MNAKVSEKGQVTIPKPLRDRLGIRAGETLDFEVSDGRLIARKAR
                     GRDPVDALYGMLELPARPDALLDDLRGPGPAP"
     gene            complement(2533..3186)
                     /locus_tag="VHF88_05945"
     CDS             complement(2533..3186)
                     /locus_tag="VHF88_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2161348.1"
                     /translation="MTSDPEQRAAETPKRGLLVDFGGVLTTNVFQSFRAFCQAEGLAP
                     DAFSDLLRSSAETRAELRKLETAQIDADEFGAYLAPLLGVADADGLMNRIFAGIGPEP
                     AMIDAVRSARGAGIRTGLISNSVGTDIYDRAVLDELFEAIVISGEVGLHKPQPEIFLL
                     GAERIGLTPAECVFVDDLRENCAGAEAVGMTAVLHRGPEQTLPELERLLGVAVGYDR"
     gene            complement(3186..4259)
                     /locus_tag="VHF88_05950"
     CDS             complement(3186..4259)
                     /locus_tag="VHF88_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX2161349.1"
                     /translation="MAADEIVDTRAEAAADEPAPLLVLEPLSEFLDEAGLGSGAIDAE
                     RIGDGHSNVTYLVRRGGERFVLRRPPRPPLPPSAHDVVREARLLTALADTAARVPRVL
                     AVCDDESVLGVPFYVMEEVPGTVITDAVPPALDSPAERSRIGEELVDALVEIHAVDWR
                     AAGLEGFGKPSGYLARQLRRFAGLWEHNKTRELPVVAEVGDWLAANVPESPPATIVHG
                     DYRLGNVMVADDPPARLVAVFDWELATIGDPLADLGYLTVTWIAPDDPDDTMFSSLTS
                     VTRLPGFPSREQLVAHYEERSGRAVGPLHWYQALALWKAAVFMEGNYKRFLAGASDDA
                     FMAVFDEGVPALAEKAREIARAG"
     gene            complement(4271..4735)
                     /locus_tag="VHF88_05955"
     CDS             complement(4271..4735)
                     /locus_tag="VHF88_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2161350.1"
                     /translation="MRVSAKADYAVRAAIELAANGNDSSRPLKGERISEAQGIPLKFL
                     ENILVDLRIGGLVNSRRGPEGGYWLAQPAEDVSVADVFRAAEGPLASVRGEKPEDLDY
                     AGNAEALRDVWIALRASLRDVLEHVTLADIAAGKLPRQVSSLTREPGAWERR"
     gene            complement(4805..5242)
                     /locus_tag="VHF88_05960"
     CDS             complement(4805..5242)
                     /locus_tag="VHF88_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX2161351.1"
                     /translation="MRLSVSADELTGVAETLVERLLQQGHDLTMHGILASGERPDWAW
                     ASEAAARDVAEGRTDQAIVCCWTGTGAAIAANKVPGIRAALCADAETARGARRWNDAN
                     VLALSLRTTSAAVLDEILEAWFAGAPSDEHDDVANIRHVDEIA"
     gene            complement(5263..7125)
                     /locus_tag="VHF88_05965"
     CDS             complement(5263..7125)
                     /locus_tag="VHF88_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX2161352.1"
                     /translation="MAAVVTLLVCGGSAHARDATVTSFDGTQIVTHFFPAAGLAPGAQ
                     APTVLMGHGWGGSGATDPEGASDATTGNTGVGPLRNAGFNVLTWDARGFGGSGGQVEV
                     DSPEHEGRDVQALIDWLALQPEARMEAPGDPLVGMSGVSYGGAIQLIAAAIDDRVDAI
                     TPTIAWHSLTRSLYRDGSVKQGWGTLLFAAGSASGNLNPQIQQAFTEGLSTGVFSDAS
                     LAFFDARGIDRLIAQIDVPTLISQGTVDTLFTLQEGIDNYAALKRNGVPLKMVWFCGG
                     HGLCNSGAGPAGLVERSVIAWLRRHLKGEPVDTGPAFEWVADDGVLRSSADYPLAWRP
                     PLRGTGSGLLPVTPGSPVTGGGQLIAASPALDAVNVQIEPASGPSELVGAPRVRITYS
                     GTALPARTHLYAQVLDVRANRVLGNQVTPIPVILDGQQRTITRELEPVAAHATPESSY
                     RVQIAAGTTVYGLQRSVGAVTLSRIDAELPVGMPVSSTAGTTGGGSSPTAATGPGQGG
                     VDRAGDEAGSERVRLVVGKARGLATARRGERPVRVVVRARGGAVRRIRVVLRNRRGKA
                     VAVSRRFGLQAGQRKVVRMGLRVDRLRAARYRVQAAGRAQGEVVRDRRALALKR"
     gene            7187..8035
                     /locus_tag="VHF88_05970"
     CDS             7187..8035
                     /locus_tag="VHF88_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2161353.1"
                     /translation="MPELPEVEIAARRLTTAAAGAMVESALAPGMVTMKTFDPPLDAL
                     AGRTLDAVQRRGKLFKVRFGDLTLLIHLMSAGRLQLWDKRASLRDKASRLLVRLDGDR
                     ELRLREFGTQQRAWAKLLPADEVDADPAVATLGPEAWPTLPPDAFAELIDQPRHLHPL
                     LRDQRTIAGIGRSWVDEILWTARLSPFKQGDDLEADEVERLRVAIGDRLGHALDHYEE
                     VIGATIPDKLPLPLEVHRRQGERCPRCGTKLEAIHFKDYVMSYCPEEQTGGRVLKDRR
                     LSKLLK"
     gene            8062..8520
                     /gene="bcp"
                     /locus_tag="VHF88_05975"
     CDS             8062..8520
                     /gene="bcp"
                     /locus_tag="VHF88_05975"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX2161354.1"
                     /translation="MDEGDKAPDFELRDQDGETVKLSDHAGETVVLYFYPRADTPGCT
                     TQACGIRDRRADYEAAGATVIGVSPDEPSALRKFADKHSLDFTLVGDPDHSVAEAYGV
                     WVEKSMYGKKYMGIQRATFIVDGDGKIAKVFPKVSPKTHDDVVLKALAEL"
     gene            complement(8517..9269)
                     /locus_tag="VHF88_05980"
     CDS             complement(8517..9269)
                     /locus_tag="VHF88_05980"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX2161355.1"
                     /translation="MTHAARTVAGTSGFGRRLGSALAVALTATAGLAAVVTASAAAAT
                     PQPRASQPLVVLQQGHVARAAPSRDARRIEYVAARRPLTGVRTVLPVLGRGNGGDWIR
                     VRLPGRPNGHAGWIRAAHTRRTSTLWRLSLDLSARRVTVRFAGRVERRFDAVIGTSAT
                     PTPLGRFFVEEGVRLAPWHAGGPYALATSARSNVLQEFAGGPGQIALHGTAGLAGTPG
                     SAASHGCIRLSTGAITWLARRIGAGVPLTVAR"
     gene            complement(9266..10438)
                     /locus_tag="VHF88_05985"
     CDS             complement(9266..10438)
                     /locus_tag="VHF88_05985"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEX2161356.1"
                     /translation="MPYPTHLRRARRAAIGLSIGLACALIPTAAQAQVDLGTAGSFVV
                     LGGQSVTNTGTSVLNGDLGVAPGTSLTGFDVAVVNGATHNNDAVAGQAQLDLTTAYDV
                     AAGQPVPPGNVLTGQDLGSRTLTSGAYRYATSAQLTGALTLDAQGNPNAQFIFEIGST
                     LTTASASSVVLINGASPCNVYWQIGSSATLGTTTAFQGNLMALATISLDNGASVLGRA
                     LARNGSVTLDDNVLTRPLCATGTSTTPDTTPTDATPPGSGTAPTPGAATTPAATPAQL
                     QARARARARARARAARRGIARISRSARDSCRDGFVARVRGRLIERAVFRLDGRRIATR
                     RGSPFRVLIPATPGAHRVRVRVTFEDATRARTMTLRYRACAAQLLEPRRGPSRYTG"
     gene            10579..11406
                     /locus_tag="VHF88_05990"
     CDS             10579..11406
                     /locus_tag="VHF88_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2161357.1"
                     /translation="MTLAPTIELLDGGRVPRLGLGTWPLDDDAAYAAVATALEQGYRL
                     IDSAARYDNEAGVGRAVADSDVPREEIVVTTKLPGADHGYEEALAGFEASRRRLGLEY
                     VDLYLIHWPLPSVDRYVDTWRAFVHLREEGLTRSIGVSNFTPAQIERIVEATGVWPAV
                     NQIELHPEFPQAEARAWHAEQGVAIESWRPLGRGVLDYPVVGRLAAAHGRTPAQIVLR
                     WHMQLGLVAIPKSGNPERIAQNIDVFDFELSDDDMAAIGALDRDNRLGGDPDTHVEL"
     gene            11430..12410
                     /locus_tag="VHF88_05995"
     CDS             11430..12410
                     /locus_tag="VHF88_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2161358.1"
                     /translation="MVPSATLGDSFRSFFDAVDQFFQGLADVRLGPLLLALLAFGIYL
                     TFRARASYNILRAAYPTEQIAFRDIWGAYFAGYGFNAVIPARGGDVVRLFLTKTAIPN
                     SSYPAVGSSFIVELGFDVAMGSLVLLFAFTQGVFPKPPDFANLNAFDLSYLAAHPRFT
                     LFLITALAVATLFSFAYFSVRVRAFWERVRQGLTIAFDRRRYLREVFAVQFVGWLFRF
                     TAFWFLLEAFNVGGSVKNVMLVLGVNAVAAAVPFTPQGAGVAQALLVKVFAGTAPAAT
                     VAAYSVGQQIAIGAFVFGVGFAALAFIFRLRSFKEVIARGREDRAAENSA"
     gene            complement(12414..12692)
                     /locus_tag="VHF88_06000"
     CDS             complement(12414..12692)
                     /locus_tag="VHF88_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161359.1"
                     /translation="MSSEDPFGGLFGDPDELRRRMAEMAEQMQGAQKVAWADNAIKLA
                     VDMTVAGIDRLNLQGNAEQQAEEVRAAMTRIFPEAVALIREAREGFGT"
     gene            12742..13551
                     /locus_tag="VHF88_06005"
     CDS             12742..13551
                     /locus_tag="VHF88_06005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2161360.1"
                     /translation="MAIPDPSKDSAALVTGASSGIGADIARSLARRGRNVVLVARREQ
                     RLSELADELVEAHGVRAETIAADLQTNAARKRLPTQVEDLGLRVDVLVNNAGFGTAGP
                     FQRLDGEREAELVRLNCEVVVDLCGRFVPTMVERGGGGVLNVASTAAFQPLPTQATYS
                     ASKAFVLTFTQALGADLHGTGVTATALCPGPVKTEFIDVAGLAKEAAILPDFVWVSSP
                     DVAEAGVDGLERGKGVVIPGLVNKATALGGHFTPRSVLLGLGRRVYRLGTG"
     gene            13576..14415
                     /locus_tag="VHF88_06010"
     CDS             13576..14415
                     /locus_tag="VHF88_06010"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX2161361.1"
                     /translation="MSAADQLEWERRWAKPAAFAAFAAAFFLVAATIVRQAVALKGNP
                     DDEREFLLAIDENSGGYLVSSGLQALSFLALVGVFLYLGRAVMARRPELPRVLLWLGL
                     IGPLLLALAGVLSDLDRLDIADRFLASGAETVQRAEDLLDDRSVLSTSLGSAGTLAIA
                     VAFVLFGINAMRVGLLTRFMGIIGAIVGALYVIPLLAGPLVVQLVWLLALGVLFLGHW
                     PGGRGPAWETGEAVEWPSGLARQPVGEGEAGAEAEPPPQGDGAADAPARTRSSRKRKK
                     KKR"
     gene            complement(14417..15100)
                     /gene="rpe"
                     /locus_tag="VHF88_06015"
     CDS             complement(14417..15100)
                     /gene="rpe"
                     /locus_tag="VHF88_06015"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX2161362.1"
                     /translation="MNEQLLRDRRVAPSILASDFARLGDQVAAVMDAGARVIHVDVMD
                     GHFVPPISIGPLIVDALRDQVHDAGGVLDVHLMVERPERHVADFASAGADSITAHFEA
                     TPNIHYALHAIRDAGCSAGLALNPGTPVDAIAALTGAYDLLLCMTVNPGWGGQPYIPA
                     SDAKIAALRERMPQGVALEVDGGIDPETAGPAAAAGASLFVAGSSIFGQPDPAAAYRA
                     VAEAAGAIV"
     gene            complement(15126..15911)
                     /gene="thyX"
                     /locus_tag="VHF88_06020"
     CDS             complement(15126..15911)
                     /gene="thyX"
                     /locus_tag="VHF88_06020"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX2161363.1"
                     /translation="MHETRPTVHLIARPQLDLDGMRAYLEDVGGESWLERRLGEQDRS
                     SPGEMLVEFGGRACYRSWEPGLNPNVTRVRTDRMEYFANILRSAHGSVLEHANYSFAL
                     RNVSRVFTHELVRHRAGSAFSQESLRYVRLTEIGFRVPPALEPVRDQVISIVEQLEEF
                     QVSAADRLGIDEEGLPFHVKKEITSALRRLAPIGLSTDIMWTANARTLRHVIEMRTAE
                     GAEEELRLVFDQIARIMQAEAPGLFQDFAQQDDGSWAPEHRKV"
     gene            complement(15949..17598)
                     /locus_tag="VHF88_06025"
     CDS             complement(15949..17598)
                     /locus_tag="VHF88_06025"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX2161364.1"
                     /translation="MPNLVLGPLLRYTGENCAVIWVETDCACEVAVLGTTSNSFQVAG
                     HHYAVVRADGLDGGRRYEYTVELDGQQVWPLLDSPFPPSTLCTRPKNPPLRIAFGSCR
                     VGASHEQPYTLRKDDDDRGRELDPLRAMALRMAHQDPDDWPDLLLLLGDQIYADEVSP
                     ETRKFVEAKRDMSVPPGPIALDFEEYTRLYRESWGDPVIRWLLSTVSSAMIFDDHDVH
                     DDWNTSAAWVEEMRATDWWDEHIVGALMSYWVYQHAGNLSPAEQDRDGLLARCHEAGD
                     AEPLLREHAERWAATTDGSRWSYHRDLNGTRLVVIDSRAGRCLKEGERSMLDEEEWSW
                     VSDQMRGDFDHLLIASSLPWLLAPGMHYLEAWNEAVCGGAWGKLAARAGEKVRQGLDL
                     EHWAAFMESFERLAELQRSVGAGEHGRPPASIVTLSGDVHHAYLSEVGFRRGSGVQSN
                     VYQAVCSPLRNPLNSRERRAIKAAASRPAHALTRALARAAGVSDPSVHWRALEDSPWF
                     DNQVASLKIAGRDIELRLDKAVPGPTDQEPGLERVLERPLV"
     gene            complement(17636..19144)
                     /locus_tag="VHF88_06030"
     CDS             complement(17636..19144)
                     /locus_tag="VHF88_06030"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX2161365.1"
                     /translation="MNLASILTEAAERSPKKVAFKLDDVELSYAALNEGSARMAGMLR
                     AAGVGPGDRVGIMLPNVPYFPVAYYGVLRLGGVVVPMNVLLKGREVRYYLEDPGAKAL
                     IAWNGFEEAAREGGEGLELETFVVTPGEFEQQLGAAEPVREAVDRNESDTAVILYTSG
                     TTGSPKGAELTQANMLTNCRIVTESVIVVNEDDRLLGALPLFHSFGQTCTMNVAINAR
                     ATVSLIPRFDPVKALEILERDQITIFEGVPTMYNAMLAVPDRDRYDVSSLRVCVSGGA
                     SLPVETLRAFEEAFGCKVLEGYGLSETSPVASFNHPHMAQKPGSIGTPIEGVEMKAID
                     DDGNEVAQGEPGEIAIRGHNVMKGYWQRPDATAEAIDGDGWFKTGDIATVDEEGYFFI
                     VDRKKDMIIRGGYNVYPREIEEILYAHPAVLEAAVVGVPHDELGEEVGAAVVLNEGAE
                     ASAEEIREHCKAEVAAYKYPRHIWFLDELPKGPTGKILKREIETPAEVLKAG"
     gene            19353..19955
                     /locus_tag="VHF88_06035"
     CDS             19353..19955
                     /locus_tag="VHF88_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161366.1"
                     /translation="MERTADEERSRADSTTQKAAWLLAFCGVILGIAATQARELLLRA
                     DELGAVLRVGGTVTLALACVLVAIAATISLRVLFAGRGQVALHEEEIAGALNQGLLTE
                     TKAYNQLRAVRVLQRQIPRMRDINHRRATELLNGFASLLVAVFFFVGHVGVFLADAHN
                     GLECGRTDRVSLAQVQQGGVRIPPCPKTTTVVDTTHLPSR"
     gene            complement(19979..21517)
                     /locus_tag="VHF88_06040"
     CDS             complement(19979..21517)
                     /locus_tag="VHF88_06040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX2161367.1"
                     /translation="MMDLLAATPSPEIDYQGLAPVLAVAGGSVVVLLASLFEGRGVQR
                     LLVPLLAAAALVVAIVFSVVNWEPGDSRPIVENALAIDTLALFMSLLFYVAGLAAIAL
                     SLRWESVQEAGAGEYYSMMLGSIAGMTLLAGAENLVTLFVGLELLSIPLYVLCASSVR
                     DRHSLESGLKYLVIGSVGSATLLYGLAFVYGASGSTDFDGIARALDGRLGIGDPLLMT
                     GIALAAVGLAFKASVAPFHQWTPDVYQGAPTPVTTFMAVATKAAAFAVLLRLFGGALF
                     DFHDDWAPALAALAVATILIGNAGAIVQRSLKRLLAWSGVAQAGYMLAGVVVATKLGL
                     QATAFYLAAYLVMNVAAFAVVIARERVSEHGDDIESLRDLSRAAPLLAWPMTIAMLAL
                     AGFPATAGFIGKFYLIDAAVAGDYAWLGIAIVVGSMISLVYYLRVIAVMWMDRLEITL
                     PTNPPRSVKPITGWSPEADPRATPEITAVALLAAAATIFLGIYPSPLFDVVRDVGTSL
                     GSLL"
     gene            complement(21520..23079)
                     /locus_tag="VHF88_06045"
     CDS             complement(21520..23079)
                     /locus_tag="VHF88_06045"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX2161368.1"
                     /translation="MLTVHLSILVFLPVVAALVGALLPARIGRWVVVAGSVAVLGYAI
                     AMIADFDGGPAGMKYVTDDEWIAELGIRYQLGVDGLNLFLVALTALLWVPAMWWSAVR
                     ETDRQKLFLFHMALAETAVLGAFLAQDLALFVLFFDLMLVPFYFLIGGWGQGDRVAAT
                     TKFVIYTLVGSLLMLAGAVALGVLTASETGNDLTFSIAALAANPLPEGSQQWIFLLFA
                     LAFLVKAPAFPFHGWVVDTYRATPTPVLVVLSAVLSKVGVYAFLRIVLPVMPDATVHF
                     QDLLLVIAVFSILYGSVLAFSQDEPRLVLAYSSIAQLGFITLGIFAIDDLGAQGAVIQ
                     MVNHGLVVGALLFVIAVLTARAAGSPSLEQMGGIALRAPVLATLFLIVILATLAMPGS
                     PNFVGEILILFGALEDKIVFGLVASIGVALAAVYAIRLFQRSMHNRENPDLVGGDLSR
                     LDLGVIAPIAAVIVALGVYPQLILERTEQATTAQVEAAAERTGDGTPARADQRRQPTG
                     EPAGSDQGEEH"
     gene            complement(23079..>24155)
                     /locus_tag="VHF88_06050"
     CDS             complement(23079..>24155)
                     /locus_tag="VHF88_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2161369.1"
                     /translation="AIAYSTMSQIGYMILGVSVFAYGAGLFHLMTHAFFKALLFMGAG
                     SVIAAMASNQSLDRMGGFRKSMPFTYVTFTAGALALAGFPLTAGFFSKDEILAYTLHR
                     GDWFVALAVVGYLTAFLTAIYAFRLVFRVFHGEPSPEARELEGGHLAHAAPTNPATGE
                     PEDTGPGFPGPEHHIAEHEGAMKAAMGPLALLAIVAGFIQIPGVTDVLEKFLEPTFAS
                     SHYVHEHPSDSAEYLGMAVGAALSIAGILVAARIYLWRPGTSAQLQARLAGVHRLLAN
                     KWYFDELYDLAIVRPTGAAGRFGRSVIESAFVQGFLVGGTVGVVRAGSSFARSIQTGE
                     LRAYAALLLVGVSAIVLYFLIAAS"
BASE COUNT         3522 a         8458 c         8721 g         3454 t
ORIGIN      
        1 cagaccggca cgggcaagac cgccgccttc gggctgccgc tgctcgacta cctcgatccg
       61 tccgatcccg ccgtccaggc gctcgtgctc accccgacgc gcgagctgtg catccaggtg
      121 acccaggcgc tgcgcgccta cggcgcccag cacggcatcg aggtcgtcgc ggtcttcggc
      181 ggtgcgccga tccgcgacca ggcctcgcgg ctcaagcagg aggcgcaggt cgtcgtcggg
      241 accgtcggcc gggtcatgga catgatccgc cgccactacc tcttcctcga ccaggcgcgc
      301 tacgtcgtgc tcgacgaggc cgacgagatg ctcgacctgg ggttcttcga ggacgtcgac
      361 gagatcctcg cccgctgccc gtccgggcgc cagaccgcgc tgttctcggc gacgatgccg
      421 ccgccgatca aaaagctcgc cgagaagcgg atgttcgatc cggtgacgat caaggtcaag
      481 gccgcgcagc tgacgatcga caccgtcgcc caattccaca tcgaggtgcc cgacaaggac
      541 aaggcggagc ggctcggcga agtgctcaag gccgagcggc ccgagcaggc gatcatcttc
      601 gcgcgcacga agatcggcgt cgaccggctc gcgcggacgc tcgaccgcca gggtctgcgc
      661 gtcaagacgc tgcacggcaa catgagccag ggcgcgcgcg acggcgtgat gatcgccttc
      721 aagggcggcc gcgagcggct gctggtggcg accgatgtcg ccgcgcgtgg gctcgatatc
      781 acgggcgtgt cgcacgtcgt caactacgac gtgccgaact cgcccgacat ctacgtccac
      841 cgcatcgggc gcaccggccg cgcgggggag accgggcggg cgatcacgct gatcacgccc
      901 aagcagcgcg aggacctcga ggcgatcgag cgccacgcga acacggagat cccggcctgg
      961 acggccccga acggcgcggc tccggagcgg ccgaagcgcg tgcgtccgcc gaaggccgcg
     1021 aagcccgagg ccgaggcgcc caaggtcgcg gccgtcgagc agcaggacga ggagcccccc
     1081 gaggcgccgc agccggagcc gcgcgagcgt ccccagccgc gccgcccgcg ccacaccaag
     1141 ccgcgaccgc gcgacggcaa ggtggcgaag ctgctgatcg gcgggggccg cgcgcagggc
     1201 ttccagcccg ccgacgtcgt cgcggcgatc gtcgacaact gccatctcga cggcgaggac
     1261 gtgcgcaagg tgcgcgtgct cgagcggttc agcttcgtcg aggtgccggc cgagcgggcg
     1321 ggcgaggtgg tcgagaaggt caacggcaac gaggtccggg gcgtgccgct ccggctggag
     1381 gtggcgaagc gatgagtcag gcgacactgc acacgacgca cgggccgatc gaggtccagt
     1441 tcttcgacga cgacgcgccc aagaccgtcg agaactttcg caagctgtcc gctgacggct
     1501 tctacgacgg tctgtcgttc caccgcgtga tcaaggactt catgatccag ggcggctgcc
     1561 cgcagggaac tggcaccggc ggccccggct acaccttcga ggacgagttc aacgaccaca
     1621 aggtcgtccg cggcgcgctc gcgatggcca acgccggccc gaacaccaac ggcagccagt
     1681 tcttcatcgt cacgaccgcc gaggcctcct ggctcgacgg caagcacacg gtctttggcg
     1741 aggtggtgtc cggcatggac gccgtcgacg cgatcgaggc cgtccccacc ggcccgggcg
     1801 accagcccga ggagccggtc gtcatcgagc gcgtcgagct cgccgacgga gccggctgag
     1861 ccgccgcagg ggaggcgact accgggtcgg ttccaggatc gtcaggccct cgaaccgtgt
     1921 gcgatagaac ccacggtcgc gggtcagtag gcgatccgcg tggatcccgg cgtgtgcggc
     1981 gaccagaaag tcggcgatga cgcggtcgcg cgtgcctccc gcgcggcggt aggcgcgcca
     2041 tgcctgcgcg gcgtcgagcg cggctcgcgc gtcgaccggg ctgtactcca gaccgaggct
     2101 gtcgagcgcc gctcgcgctg ctcccgcgct ctcaaacgag gcgcccgtct cggcccacac
     2161 gatgtcgcag gcgatcagct tcccctcctg ccgcgcgccg cggatggcct cgcgcgacgc
     2221 agggccgaac tcagggtcgc cgccgagcag atcgagcagg acgttggtgt ccacggcggt
     2281 gatcagggcg ccggtccggg accgcggagg tcgtcgagca gcgcatctgg tcgcgccggc
     2341 agctcgagca tcccgtagag agcatcgacc ggatctcgcc cgcgggcctt acgggcgatc
     2401 agccgcccat cgctcacttc gaagtcgagc gtctcgcccg cccggattcc cagccgatcg
     2461 cgcagcggct ttggaatcgt gacctggccc ttctcggata ccttcgcatt catactgcaa
     2521 gagtagcaat tcctacctgt cgtatcctac cgctactccc agcagccgct caagctcggg
     2581 cagcgtctgt tccggcccgc gatgcagcac tgccgtcatg ccgacggcct cggcgccggc
     2641 gcagttctcc cgcaggtcgt cgacgaacac acactccgcg ggcgtcagcc cgattcgctc
     2701 ggcgcccaga aggaaaatct ccggctgggg cttgtggagg ccgacctcgc cggagatgac
     2761 gatcgcctcg aagagctcgt cgaggacggc acggtcgtag atgtcggtgc cgacggagtt
     2821 cgagatcagg ccggtgcgga tgcccgcgcc gcgggcgctg cgtacggcgt cgatcatcgc
     2881 tggctccggg ccgataccgg cgaatatgcg gttcatcagt ccgtcggcgt cggccacgcc
     2941 gagcaggggc gcgaggtatg cgccgaactc gtcggcgtcg atctgggcgg tctcgagctt
     3001 gcgcagctcc gcccgcgtct cggcactcga gcgcagcagg tcggagaagg cgtcgggtgc
     3061 cagcccctct gcctggcaga acgcgcgaaa ggactggaag acgttcgtgg tgagcacgcc
     3121 gccgaagtcg accaacaggc cgcgcttcgg cgtctcggcg gcgcgctgct cggggtcgga
     3181 ggtcactagc ccgcccgcgc gatctcgcgc gccttctccg cgagcgccgg tacgccctcg
     3241 tcgaagacgg ccatgaaggc gtcgtcgctc gcgccggcga ggaagcgctt gtagttgccc
     3301 tccatgaaga cggctgcctt ccagagagcg agcgcctggt accagtggag cgggccgacc
     3361 gctcgccccg atcgctcctc gtagtgggcg acgagctgtt ctctgctcgg gaagccgggc
     3421 aggcgggtga ccgacgtcag cgagctgaac atcgtgtcgt ccgggtcgtc gggggcgatc
     3481 caggtgacgg tcaggtagcc cagatcggcg agcgggtcgc cgatcgtcgc gagctcccag
     3541 tcgaagacgg cgaccaggcg agccggcggg tcgtcagcca ccatcacgtt gccgagccgg
     3601 tagtcgccgt ggacgatggt cgccggtggg gactcgggca cgttcgccgc cagccagtcg
     3661 ccgacctcgg ccacgaccgg cagctcgcgc gtcttgttgt gctcccagag gccggcgaag
     3721 cggcgaagct ggcgcgcgag gtagccgctc ggcttgccga agccctcgag cccggcagcg
     3781 cgccagtcga cggcatggat ctcgacgagg gcgtcgacga gctcctcgcc gattcgcgac
     3841 ctttcggccg gggagtcgag cgccggcggg acggcgtcgg tgatcacggt ccctggcacc
     3901 tcctccatca cgtagaacgg gacgccgagg accgactcgt cgtcgcacac cgcgagcacg
     3961 cgcggtacgc gggcggccgt gtcggcgagc gccgtcagca gccgcgcctc gcgcaccacg
     4021 tcgtgcgccg acggcggcag cggcggacgc gggggacggc gcaggacgaa tcgctcgccg
     4081 ccgcggcgca ccaggtacgt cacattcgag tgcccgtcac cgatgcgctc ggcgtcgatt
     4141 gcgcccgacc cgagccccgc ttcgtcgagg aactccgaca gcggctcgag gaccagcagc
     4201 ggcgccggct cgtcagccgc cgcctccgcg cgcgtgtcga cgatctcgtc ggctgccatg
     4261 cggccgtggt ctagcgacgc tcccaggcgc cgggctcgcg cgtcagcgac gacacctgcc
     4321 gcggcagctt gccggcggcg atgtctgcga gcgtcacgtg ctcgaggacg tcacgcaagc
     4381 tcgcgcgcag ggcgatccag acgtcgcgca gcgcctcggc gttgccggcg tagtcgaggt
     4441 cctcgggctt ctcgccgcgg acgctggcga gcgggccctc cgccgcgcgg aagacgtccg
     4501 cgacgctgac gtcctccgcc ggctgggcga gccagtagcc gccctcgggg ccgcggcggc
     4561 tgttgaccag gccgccgatc cgcaggtcga cgaggatgtt ctcgaggaac ttcagcggga
     4621 tcccctgcgc ctcggagatc cgctcgccct tcagcggtct ggaggagtcg ttgccgttgg
     4681 cggccagctc gatcgccgca cggactgcgt agtcggcctt ggcggagacg cgcatggacg
     4741 cgcattctcc accaaatccc cttcaaagcg tcgcaatgca cgcgacgctg cgcgcgagga
     4801 gcgctcaggc gatctcgtcg acgtggcgga tgttggccac gtcgtcgtgt tcgtcgctcg
     4861 gcgcgccggc gaaccaagcc tcgaggatct cgtcgagcac ggccgcgctg gtcgtgcgca
     4921 gcgagagcgc cagcacgttg gcgtcgttcc agcgccgcgc cccgcgcgcg gtctcggcgt
     4981 ccgcgcagag cgcggcgcgg atgccgggga ccttgttggc ggcgatcgcg gcaccggtcc
     5041 cggtccagca gcagacgatc gcctggtcgg tgcggccctc ggcgacgtcg cgggctgcgg
     5101 cctcgctcgc ccacgcccag tcgggtcgtt cgccgctcgc gaggatcccg tgcatcgtca
     5161 ggtcgtggcc ctgctgcagc aggcgctcga ccagggtctc cgccaccccg gtcagctcgt
     5221 cggcggacac gctcagtcgc acggttcgag cctagccggg gcctaccgct tgagcgcaag
     5281 cgcccggcgg tcacgcacga cctcgccctg cgcgcggccc gccgcttgga cgcggtagcg
     5341 cgcggcgcgc aggcggtcga cccgcagccc catgcgcacg accttccgct ggcccgcctg
     5401 cagcccgaac ctccggctga cggcgacagc cttgccgcgc cggttgcgca gcacgacccg
     5461 gatccgccgt accgcgccgc cgcgcgcacg gacgacgacg cgtaccgggc gctcgccgcg
     5521 ccgcgccgtc gccagcccgc gggccttgcc gacgaccagg cggacccgtt cgctgccggc
     5581 ctcgtcgccg gcgcggtcaa cgcctccctg gccggggccg gtcgccgctg tcggcgacga
     5641 gccgcctccg gtcgtgcctg ccgtcgagct caccggcatc ccgacgggca gctccgcgtc
     5701 gatacgcgac agcgtgaccg ctccgacgct gcgctggagc ccgtagacgg tggtgcccgc
     5761 cgcgatctgc acccggtagc tcgactcggg cgtcgcgtgg gcggcgaccg gctcgagctc
     5821 acgcgtgatc gtccgctgct ggccgtcgag gatgaccggg atcggcgtca cctggttgcc
     5881 gagcacgcgg ttggcgcgga cgtcgagcac ctgggcgtag agatgggtgc gcgccgggag
     5941 cgccgtgccc gagtacgtga tgcgcacgcg cggcgccccg acgagctcgc tgggaccact
     6001 cgccggctcg atctggacgt tgacggcgtc gagcgcgggg ctcgcggcga tcagctggcc
     6061 gccgcccgtc accggtgatc ccggcgtcac cggaagcagg cccgagccgg tcccgcgcag
     6121 cggcggtcgc caggcgagcg ggtagtcggc cgagctgcgc agcacaccgt cgtcggcgac
     6181 ccactcgaac gccgggccgg tgtcgaccgg ctcgcccttc aggtgccggc gcagccacgc
     6241 gatgaccgag cgctcgacca ggcccgcggg cccggcgccc gagttgcaga ggccgtgccc
     6301 gccgcagaac cagaccatct tcagcggcac gccgttgcgc ttcagcgccg catagttgtc
     6361 gatcccctcc tggagcgtga acagcgtgtc gacggtgccc tgggagatca gcgtcggcac
     6421 gtcgatctgg gcgatcagac ggtcgatgcc gcgcgcgtcg aagaacgcca ggctcgcgtc
     6481 ggagaacacg ccggtgctca gcccctcggt gaacgcctgc tggatctggg ggttcaggtt
     6541 gccgctggcc gagcccgctg cgaacagcag cgtcccccag ccctgcttga ccgacccgtc
     6601 gcggtagagg ctgcgggtca gcgagtgcca ggcgatcgtc ggggtgatcg cgtcgacgcg
     6661 gtcgtcgatc gccgccgcga tcagctggat cgcgccgccg tagctgacgc cgctcatgcc
     6721 gacgagcggg tcgcccggcg cctccatccg cgcctcgggc tgcagcgcca gccagtcgat
     6781 cagcgcctga acgtcgcggc cctcgtgctc gggcgagtcg acctcgacct ggccgccgga
     6841 gccgccgaac ccgcgcgcat cccaggtcag cacgttgaag ccggcgttgc gcagcggccc
     6901 gacgccggtg ttgccggtcg tcgcgtcaga ggcgccctca gggtcggtcg cgccgctgcc
     6961 gccccagccg tggcccatca ggacggtcgg ggcctgcgcc ccgggcgcca ggccggcggc
     7021 ggggaagaag tgggtgacga tctgcgttcc gtcgaaggag gtgaccgtgg cgtctcgcgc
     7081 gtgggccgag ccgccgcaca cgagcagggt gacgaccgct gccacagccg ctgcaactcg
     7141 actccccatg cggctcggac gtacccagcc ggcctactct tcaagcgtgc ccgagctgcc
     7201 cgaggtcgag atcgccgcgc gccgcctgac gaccgcggct gctggggcga tggtcgagtc
     7261 ggcgctcgcc cccggcatgg tcacgatgaa gaccttcgac ccgcccctcg acgcgctcgc
     7321 cgggcgcacg ctcgacgccg tgcagcggcg cggcaagctg ttcaaggtcc gcttcggcga
     7381 tctcacgctc ctgatccacc tgatgtcggc cggccggctg cagctgtggg acaagcgggc
     7441 gtcgctgcgc gacaaggcgt cgcggctgct ggtccgcctc gacggcgacc gcgagctgcg
     7501 gctgcgcgag ttcggcaccc agcagcgcgc gtgggcgaag ctgctcccgg ccgacgaggt
     7561 cgacgccgac cccgccgtcg ccacgctcgg gccggaggcc tggccgacgc tgccgcccga
     7621 cgccttcgcc gagctgatcg accagccgcg ccacctccac ccgctgctgc gcgaccagcg
     7681 cacgatcgcc ggcatcggcc gctcgtgggt cgacgagatc ctctggaccg cgcggctgtc
     7741 gccgttcaag cagggcgacg acctcgaagc cgacgaggtc gagcggctgc gcgtcgcgat
     7801 cggcgaccgg ctcggccacg cgctcgacca ctacgaggag gtgatcggcg cgacgatccc
     7861 cgacaagctg ccgctgccgc tggaggtgca ccgccgccag ggcgagcggt gcccgcgctg
     7921 cggcaccaag ctcgaggcga tccacttcaa ggactacgtc atgtcctact gccccgagga
     7981 gcagaccggc ggccgggtgc tcaaggaccg ccgcctgtcg aagctgctga agtagcgccg
     8041 ccccgacagc gaaaggaccc gatggacgaa ggcgacaagg cccccgactt cgagctgcgc
     8101 gaccaggacg gcgagaccgt gaagctgtca gaccacgccg gcgagacggt cgtcctctac
     8161 ttctacccgc gcgccgacac ccccggctgc acgacgcagg cctgcggcat ccgcgaccgc
     8221 cgcgccgact acgaggccgc cggcgccacg gtgatcggcg tctcaccgga cgagccctcc
     8281 gccctgcgca agttcgccga caagcactcg ctcgacttca cgctcgtcgg cgaccccgac
     8341 cactccgtcg ccgaggccta cggcgtctgg gtcgagaagt cgatgtacgg caagaagtac
     8401 atgggcatcc agcgcgccac cttcatcgtc gacggcgacg gcaagatcgc caaggtcttt
     8461 cccaaggttt cgccgaagac ccacgacgac gtggtgctga aggcgctggc ggagctctag
     8521 cgagcgacgg tcagcggcac gccggcgccg attcgtcgcg ccagccaggt gatggcgccg
     8581 gtgctgaggc ggatgcagcc gtgcgaggct gcgctgccgg gcgtgcccgc gaggccggct
     8641 gtcccgtgga gggcgatctg gccggggccg cccgcgaact cttgcaggac gttggagcga
     8701 gcgctggtgg cgagcgcgta cgggccgccg gcgtgccagg gggcgagccg aacgccctcc
     8761 tcgacgaaga agcggccgag cggcgtgggc gtcgccgagg ttccgatcac ggcgtcgaat
     8821 cggcgctcga cgcgtcccgc gaaccgcacc gtgacgcgac gggcggagag gtcgagcgag
     8881 aggcgccaga gcgtcgacgt ccgtctggtg tgagcggcgc ggatccagcc ggcgtggccg
     8941 ttcggccgtc cggggagccg gacgcggatc cagtcgccgc cgttcccgcg gccgagcacg
     9001 ggaaggaccg ttcggacgcc ggtcagcggc cggcgggccg cgacgtattc gatccggcgg
     9061 gcgtcgcggc tcggtgcggc gcgggcgaca tggccctgct ggaggacgac gagcggctgg
     9121 ctggctcgcg gctgcggcgt ggcggccgct gcgctcgccg tgaccacggc ggccaggccg
     9181 gcggtcgctg tcagcgcgac cgccagcgcc gagcccaacc tccggccgaa gccggaggtt
     9241 ccagcgaccg tgcgtgcggc gtgggtcacc cggtgtaccg cgacggtccg cgtcgcggct
     9301 cgagcagctg tgcggcgcag gcgcggtagc gcagcgtcat ggtcctggcg cgggtggcgt
     9361 cctcgaatgt cacgcgcacg cggaccctgt gggctcccgg tgtcgccggt atcagcacgc
     9421 ggaacggcga gccgcggcgg gtggcgatcc gcctgccgtc gagcctgaac acggcgcgct
     9481 cgatcaggcg accgcggacc cgtgccacga acccatcgcg gcaggagtcg cgcgcggagc
     9541 ggctgatgcg ggcgatacct cggcgggcgg ccctggcccg tgcccgagct cgtgctctgg
     9601 cacgcgcctg cagttgagcc ggggtagcgg ctggcgtggt tgccgcccct ggtgtggggg
     9661 cggtccccga ccctggcggg gtcgcgtcgg tcggcgtcgt gtccggcgtt gtcgaggttc
     9721 cggtcgcgca cagcggccgg gtcaggacgt tgtcgtcgag cgtgacgctg ccgttgcggg
     9781 cgagcgcccg tcccagcacc gacgcgccgt tgtcgagcga gatcgtcgcg agcgccatca
     9841 ggttgccctg gaaggcggtc gtcgtgccga gcgtggccga gctgccgatc tgccagtaga
     9901 cgttgcacgg cgaagcgccg ttgatcagca cgaccgagct cgccgaggcc gtcgtcagcg
     9961 tggagccgat ctcgaagatg aactgcgcgt ttggattgcc ctgtgcgtcg agggtcagtg
    10021 cgccggtcag ctgagcggac gtggcgtacc ggtaggcgcc gctggtcagc gtgcggctgc
    10081 cgaggtcctg gccggtgagg acgttgccag gcgggaccgg ttggccggca gccacgtcat
    10141 aggcggtcgt caggtcgagc tgggcctggc cggcgacggc gtcattgttg tgcgtcgccc
    10201 cgttgacgac cgcgacgtcg aagccggtga gcgacgtgcc gggcgcgact ccgaggtcgc
    10261 cgttgagcac cgaggtgccg gtgttggtga cgctctggcc gccgaggacg acgaacgagc
    10321 cggcggtgcc gaggtcgacc tgcgcctgag cggccgtcgg gatcagtgcg cacgccaatc
    10381 cgatgctgag gccgattgcg gcacgccggg cgcgcctgag atgggtcggg tagggcatgg
    10441 gccctccttg atgaggagca gcccggacaa cgttcctcgt cgagagaacc agctctggtg
    10501 ttcgacctgt atcggcaggg tccggccgag cttgagcaca gccgatagcg gtcgcggctg
    10561 cggggtaagg ggatctccat gaccctcgcg ccgacgatcg agctgctcga cggcgggcgg
    10621 gttccccggc tgggcctggg gacctggccg ctggacgacg acgcggccta cgcggcggtc
    10681 gcgacggcgc tcgagcaggg ctatcggctg atcgacagcg cggcgcgcta cgacaacgag
    10741 gcgggcgtcg ggcgggcggt cgcggattcg gacgtgccgc gcgaggagat cgtcgtgacg
    10801 acgaagctgc cgggcgccga tcacggctac gaggaggcgc tggcgggatt cgaggcaagc
    10861 cggcggcggc tcgggctcga gtatgtcgac ctctacctga tccactggcc gctgccgagc
    10921 gtcgatcgct acgtcgacac gtggcgggcc ttcgtgcacc tgcgcgagga ggggctgacc
    10981 aggtcgatcg gggtgtcgaa cttcacgccg gcccagatcg agcggatcgt ggaggcgacg
    11041 ggcgtttggc cggccgtcaa ccagatcgag ctgcatccgg agttcccgca ggctgaggcg
    11101 cgggcgtggc atgcggagca gggggtggcg atcgagtcgt ggcggccgct cgggcgcggc
    11161 gtgctggact accccgtggt cgggcggctc gccgcggcgc acggacggac gccggcgcag
    11221 atcgtgctgc gctggcacat gcagctcggg ctggtggcga ttccgaagtc gggcaacccg
    11281 gagcggatcg ctcagaacat cgacgtcttc gacttcgagc tgagcgacga cgacatggcg
    11341 gcgatcggcg cgctggatcg cgacaaccgc ctcggcggcg atccggacac ccacgtcgag
    11401 ctgtaggcgc ctcgtagtct ccccggggca tggtccccag cgcgaccctc ggcgactcct
    11461 tccgctcgtt cttcgacgcc gtcgaccagt tcttccaggg cctggccgac gtgcggctcg
    11521 gcccgctgct gctggcgctg ctcgccttcg ggatctacct gaccttccgg gcgcgcgcct
    11581 cctacaacat cctgcgcgcc gcctacccga ccgagcagat cgccttccgc gacatctggg
    11641 gggcctactt cgccggctac ggcttcaacg cggtgatccc ggcgcgcggc ggcgacgtcg
    11701 tgcggctctt cctgaccaag accgcgatcc cgaactcgag ctatccggcg gtcggctcga
    11761 gcttcatcgt cgagctgggc ttcgacgtgg cgatgggcag cctggtgctg ctgttcgcgt
    11821 tcacccaggg cgtcttcccg aagccgcccg acttcgccaa cctgaacgcc ttcgacctct
    11881 cctacctcgc cgcgcacccg cgcttcacgc tgttcctgat caccgcactg gctgttgcga
    11941 cgctgttctc gttcgcgtac ttctccgtcc gcgtgcgcgc gttctgggag cgggtgcggc
    12001 agggcttgac gatcgccttc gaccggcggc gctacctgcg cgaggtcttc gccgtgcagt
    12061 tcgtcggctg gctgttccgt ttcaccgcgt tctggttcct gctcgaggcg ttcaacgtcg
    12121 gcgggtcggt caagaacgtg atgctggtgc tcggcgtcaa cgcggtggcg gcggccgtgc
    12181 ccttcacgcc gcagggagcg ggcgtcgccc aggcgctgct ggtcaaggtg ttcgcgggga
    12241 ccgcccccgc cgcaacggtc gccgcctact cggtcggcca gcagatcgcg atcggcgcct
    12301 tcgtcttcgg cgtcggcttc gccgcgctcg cgttcatctt ccgcctgcgc agcttcaagg
    12361 aggtcatcgc ccgcggtcgc gaggaccgcg ccgccgaaaa ctcagcctga cggtcaggtg
    12421 ccgaagccct cgcgggcctc gcggatgagc gccaccgcct cggggaagat ccgcgtcatc
    12481 gcggcgcgga cctcctcggc ctgctgctcg gcgttgcctt ggaggttgag ccggtcgatc
    12541 ccggcgacgg tcatgtcgac cgcgagcttg atcgcgttgt cggcccaggc gaccttctgg
    12601 gctccctgca tctgctcggc catctcggcc atccggcgcc gcagctcgtc ggggtcgccg
    12661 aacagtccgc cgaatggatc ctctgatgac atgccgacga ttctagggac cgcgccgccg
    12721 cggcctggat aggctgcccg gatggccatt ccagacccat cgaaggactc ggcggcactc
    12781 gtcaccgggg cgtcgtcggg catcggcgcc gacatcgccc gctcgctggc gcggcgcggc
    12841 cgcaacgtcg tgctggtcgc gcgccgcgag cagcggctga gcgagctcgc ggatgagctc
    12901 gttgaggcgc acggggtgcg cgccgagacg atcgccgccg acctccagac gaatgccgcg
    12961 cgcaagcgac tgccgactca ggttgaggac ctagggctgc gtgtcgacgt gctcgtcaac
    13021 aacgccggct tcgggaccgc ggggcccttc cagcggctcg atggcgagcg cgaggcggag
    13081 ctggtgcggc tgaactgcga ggtcgtcgtg gacctctgtg ggcgcttcgt gccgacgatg
    13141 gtcgagcgcg gcggcggcgg ggtgctcaac gtcgcctcga cggccgcctt ccagccgctg
    13201 ccgacgcagg ccacctacag cgccagcaag gcgttcgtcc tcacgttcac gcaggcgctt
    13261 ggagccgatc tgcacggcac cggagtgacg gctaccgcac tatgccccgg tccggtgaag
    13321 acggagttca tcgacgtggc cggtctggcg aaggaggcgg cgatcctgcc cgacttcgtc
    13381 tgggtctcct cgcccgacgt cgccgaggcc ggcgtcgacg gcttggagcg cggcaagggc
    13441 gtagtgattc cgggcctcgt gaacaaggcg acggcgctcg gcggccactt cacgccgcgc
    13501 agcgtgctgc tcgggctggg ccggcgcgtc taccggctcg gcacgggcta gccgcgcgtg
    13561 acagcatccc gcgccgtgtc ggccgcagac cagctcgagt gggagcgccg ttgggcgaag
    13621 ccggcggcgt tcgcggcatt tgcggcggcg ttcttcctcg tcgccgcgac gatcgtccgc
    13681 caggcggtcg ccctgaaggg caacccggac gacgagcgtg agttcctgct cgcgatcgac
    13741 gagaactcag gcggctacct cgtctcgtcg ggcctccagg cgctgagctt cctggcgctg
    13801 gtcggcgtct tcctctacct cggccgcgcc gtcatggcgc gacggccgga gcttccgcgg
    13861 gtcctgctct ggctcgggct gatcggtccg ctgctgctcg cgctcgccgg tgtcctgtcg
    13921 gacctcgacc gcctcgacat cgccgaccgc ttcctggcct cgggcgccga gacggtccag
    13981 cgggcggagg acctgctcga cgatcgcagc gtgctctcca cctcgctcgg ctcggccgga
    14041 acgctcgcga tcgcggtggc cttcgtgctg ttcgggatca acgcgatgcg cgtcgggctg
    14101 ctgacgagat tcatggggat catcggcgcg atcgtcggcg cgctttacgt gattccgctg
    14161 ctcgccggcc cactggtggt ccagctggtc tggctgctcg cgctcggcgt tctcttcctg
    14221 ggccactggc caggcggccg cgggccggcg tgggagacgg gcgaggccgt cgagtggccg
    14281 tcggggctgg cccgccagcc ggtcggggag ggcgaggccg gcgcggaggc cgagccgccg
    14341 ccgcaaggtg acggcgctgc tgacgcaccc gcccgcacgc gctcgtcgcg caagcgcaag
    14401 aagaaaaagc gctagctcag acgatcgcgc cggccgcctc ggccacggcg cgataggcag
    14461 cggccgggtc gggctggccg aagatggagg agccagcgac gaagagagag gcgcctgcag
    14521 cggcggcggg gccggccgtc tccggatcga tgccgccgtc gacctcgagc gcgacgccct
    14581 ggggcatccg ctcgcgcagg gcggcgatct tggcgtcgga agcggggatg tagggctggc
    14641 cgccccagcc tgggttgaca gtcatgcaga gcagcaggtc gtacgcgccg gttagcgcgg
    14701 cgatggcgtc gaccggcgtc ccggggttca gcgccagccc ggcgctgcac cccgcgtcgc
    14761 ggatcgcgtg cagcgcgtag tggatgttcg gtgtcgcctc gaagtgggcg gtgatcgagt
    14821 ctgcgccggc gctcgcgaag tccgcgacgt ggcgctccgg ccgctcgacc atcaggtgga
    14881 catcgaggac gccgcccgcg tcatggacct gatcgcgcag cgcgtcgacg atcaacggcc
    14941 cgatcgagat cggcgggacg aagtgaccgt ccatcacgtc gacgtgtatc acccgcgcgc
    15001 cggcgtccat cacggcggcc acctggtccc cgagccgcgc gaagtccgac gcgaggatcg
    15061 acggggcgac ccggcggtcg cgaagcagct gctcgttcac ggctggcgcg acgctaccgg
    15121 acggctcaga ccttgcggtg ctccggggcc caggacccgt cgtcctgctg ggcgaagtcc
    15181 tggaagaggc cgggggcctc ggcctgcatg atgcgggcga tctggtcgaa gacgaggcgc
    15241 agctcctcct cggcgccctc ggcggtgcgc atctcgatca cgtggcggag tgtgcgggcg
    15301 ttggcggtcc acatgatgtc ggtgctgagg ccgatcgggg cgaggcggcg cagggcggag
    15361 gtgatctcct tcttgacgtg gaaggggagg ccctcctcgt cgatcccgag ccggtcggcg
    15421 gcgctgacct ggaactcctc cagctgctcg acgatcgaga tgacctggtc gcgcaccggc
    15481 tcgagcgccg gcggcacgcg gaagccgatc tcggtcagcc gcacgtagcg caggctctcc
    15541 tgactgaacg ccgagccggc gcggtggcgg acgagctcgt gggtgaagac gcgcgacacg
    15601 ttgcgcagcg cgaacgagta gttggcgtgc tcgagcaccg agccgtgcgc gctgcgcagg
    15661 atgttcgcga agtactccat ccggtcggtc cgcacgcggg tgacgttcgg gttcagcccc
    15721 ggttcccagc tgcgatagca ggcgcggccg ccgaactcga cgagcatctc gcccgggctc
    15781 gaccggtcct gctcgccgag ccggcgctcg agccacgact cgccgccgac gtcctcgagg
    15841 tacgcccgca tgccgtcgag gtcgagctgc ggacgggcga tcaggtggac ggtcgggcgg
    15901 gtctcgtgca cgggctggcg accctacgcc gaagctcgga cgaaacgctc agacgagcgg
    15961 gcgctcgagc acgcgctcga gccccggctc ctgatcggtc ggtcccggga ccgccttgtc
    16021 gagccgcagc tcgatgtcac gcccggcgat cttcagcgac gcgacctggt tgtcgaacca
    16081 cggactgtcc tcgagtgccc gccagtggac cgaggggtcg ctcaccccag cggcgcgcgc
    16141 cagcgcccgc gtcagcgcat gcgccggccg tgacgccgcg gccttgatcg cgcgccgctc
    16201 gcggctgttg agcgggttgc gcagcggcga gcagaccgcc tggtagacgt tgctctgcac
    16261 ccccgagccg cgccggaagc cgacctcgct caggtaggcg tggtggacat cgccggagag
    16321 cgtgacgatc gacgccggcg gccggccgtg ctcgccggcg cccacggagc gctgcagctc
    16381 cgccagccgc tcgaaggact ccatgaacgc cgcccagtgc tcgaggtcga gcccctggcg
    16441 cactttctcg cccgcccgcg ccgcgagctt gccccacgcg ccgccgcaga ccgcctcgtt
    16501 ccacgcctcg aggtagtgca tcccgggcgc gagcagccac ggcagcgagc tggcgatcag
    16561 caggtggtcg aagtcgccgc gcatctggtc ggagacccag ctccactcct cctcgtcgag
    16621 catcgagcgc tcgccctcct tcaggcagcg acctgcgcgc gagtcgatca ccaccagccg
    16681 cgtgccgttg aggtcgcggt ggtagctcca gcggctgccg tcggtggtgg ccgcccagcg
    16741 ttcggcgtgc tcgcgtagca gcggctcggc gtcgccggcc tcgtggcagc gggccagcag
    16801 gccgtcgcgg tcctgctccg cggggctgag gttgccggcg tgctggtaga cccagtagga
    16861 catcagcgcg ccgacgatgt gctcgtccca ccagtcggtc gcgcgcatct cctcgaccca
    16921 ggccgccgac gtgttccagt cgtcgtgcac gtcgtggtcg tcgaagatca tcgcgctcga
    16981 cacggtcgag agcagccagc ggatcacggg atcgccccac gattcgcggt agaggcgcgt
    17041 gtactcctcg aagtcgagcg cgatcgggcc cgggggcacg ctcatgtcgc gcttggcctc
    17101 gacgaacttg cgcgtctcgg gcgacacctc gtcggcgtag atctgatcgc cgagcagcag
    17161 cagcaggtcg ggccagtcgt cggggtcctg gtgggccatc cgcagcgcca tcgcgcgcag
    17221 ggggtcgagc tcgcgtccgc ggtcgtcgtc gtccttgcgg agcgtgtacg gctgctcgtg
    17281 cgaggccccg actcggcacg agccgaaggc gatccgcagc ggcgggttct tcggccgcgt
    17341 gcagagggtc gaagggggga acggcgagtc cagcagcggc catacctgct ggccgtcgag
    17401 ctcgacggtg tactcgtagc gccgcccgcc gtcgagtccg tcggctcgga cgacggcata
    17461 gtgatggccg gcgacctgga agctgttcga ggtcgtgccg aggacggcga cctcacatgc
    17521 gcaatcggtc tcgacccaga tgaccgcgca gttctcgccg gtgtaacgca gcagggggcc
    17581 gaggaccagg ttcggcatcg cctcaccgta cccagcggcg gctgggaaga cgccgctagc
    17641 ccgctttcag cacctccgcg ggcgtctcga tctcgcgctt gaggatcttg ccggtcggcc
    17701 ccttcggcag ctcgtcgagg aaccagatgt ggcgcgggta cttgtaggcg gcgacctccg
    17761 ccttgcagtg ctcgcggatc tcctcggcgg acgcctcggc gccctcgttg aggacgaccg
    17821 ccgcgccgac ctcctcgccg agctcgtcgt gagggacgcc gaccacggcg gcctcgagca
    17881 cggcggggtg cgcgtagagg atctcctcga tctcgcgcgg gtagacgttg tagccgccgc
    17941 ggatgatcat gtccttcttg cggtcgacga tgaagaagta gccctcctcg tcgacggtcg
    18001 cgatgtcgcc ggtcttgaac cagccgtcgc cgtcgatcgc ctcggccgtc gcgtccggcc
    18061 gctgccagta gcccttcatc acgttgtggc cgcggatcgc gatctcgccc ggctcgccct
    18121 gcgccacctc gttgccgtcg tcgtcgatcg ccttcatctc gacgccctcg atcggggtgc
    18181 cgatcgagcc gggcttctgc gccatgtgcg ggtggttgaa cgacgccacg ggcgacgtct
    18241 cggacaggcc gtagccctcc agcaccttgc agccgaaggc ctcctcgaac gcgcgcagcg
    18301 tctcgaccgg caacgacgcg ccgccggaca cgcagacgcg cagcgacgag acgtcgtagc
    18361 ggtcgcggtc aggcaccgcg agcatcgcgt tgtacatcgt cggcacgccc tcgaagatcg
    18421 tgatctggtc gcgctcgagg atctccagcg ccttgaccgg gtcgaagcgc gggatcagcg
    18481 agaccgtagc gcgcgcgttg atcgccacgt tcatcgtgca cgtctggccg aacgagtgga
    18541 acagtggcag cgcgccgagc agccggtcgt cctcgttcac gacgatcacc gactcggtca
    18601 cgatccggca gttggtcagc atgttcgcct gggtcagctc ggcgcccttc gggctgccgg
    18661 tggtcccaga cgtgtagagg atcaccgcgg tgtcgctctc gttgcggtcg acggcctcgc
    18721 gcaccggctc ggccgcgccg agctgctgct cgaactcgcc cggcgtgacg acgaacgtct
    18781 cgagctcgag gccctcgccc ccctcgcgag cggcctcctc gaagccgttc cacgcgatca
    18841 gcgccttcgc acccggatcc tcgaggtagt acctgacctc gcgtcccttc agcagcacgt
    18901 tcatcggcac gaccaccccg cccagccgca gcacaccgta gtaggcgacc gggaagtacg
    18961 gcacgttcgg cagcatgatc ccgacgcggt cgccgggccc cacgccggcc gcccgcagca
    19021 tcccagccat ccgcgcgctg ccctcgttca gcgcggcgta gctcagctcg acgtcgtcga
    19081 gcttgaacgc caccttcttc gggcttcgct cagccgcctc ggtcaggatg ctcgccaggt
    19141 tcattcgctc cgctcctccc tcggttcccg tgagatgaac ctaatgcccg ccggtggatc
    19201 agtccacacc gcgtcatggc ctgtagacac ggatgaggta ccgtcgccat gtggagggca
    19261 tctggaggtg gagggatcta cggccacgca gtcgcgagag tctccgacga gcggagccag
    19321 acgccggcgc cgtacaggca attcttgact tgttggaacg taccgccgac gaggagcgct
    19381 cccgtgccga tagcacgact cagaaggcgg cgtggttgtt ggcgttctgc ggcgtgatcc
    19441 tcggcatcgc ggcgacgcag gcccgcgagc ttctactgcg cgctgacgag ctgggggcag
    19501 tcctccgcgt aggggggacg gtgacgctcg cgcttgcttg cgtcctagta gcgatcgcgg
    19561 ccacgatctc actgcgggtg ctcttcgcag gcaggggcca agtcgcgtta catgaggagg
    19621 agattgcggg tgctttgaat caaggcctcc tcaccgagac gaaggcctac aaccagctac
    19681 gagcggtgcg cgtcttgcag aggcagatcc cacgcatgcg agacatcaat caccgacgcg
    19741 cgactgagct gctgaacggg ttcgcgagct tgctggtcgc ggtgttcttc ttcgtgggac
    19801 acgttggagt ctttctcgcg gatgctcaca acggtctcga gtgcggacgg acggatcgcg
    19861 tgtcgctcgc gcaagtgcag caaggcgggg tgcgtatccc tccctgtccg aagacgacca
    19921 cggtcgtcga cacgacccac ctgccttcgc gctgacctcg cggcggatcg tcggactgct
    19981 agagcagcga gccgagtgac gtgccgacgt ctctgacgac gtcgaagagc ggcgaggggt
    20041 agatgcctag gaagatcgtg gcggcggccg cgaggagggc gacggcggtg atctcggggg
    20101 tggcgcgcgg gtcggcctcc ggggaccagc cggtgatcgg cttgacgctg cgcggcgggt
    20161 tggtcgggag ggtgatctcg aggcggtcca tccacatgac ggcgatcacg cgcaggtagt
    20221 agacgagcga gatcatcgac ccgacgacga tcgcgatccc aagccaggcg tagtcgccgg
    20281 ccaccgcggc gtcgatcaaa tagaacttgc cgatgaagcc ggcggtggcg gggaagccgg
    20341 cgagcgcgag catcgcgatc gtcatcggcc aggccagcag tggggcggcg cgcgagaggt
    20401 cgcgcaacga ctcgatgtcg tcgccgtgct ccgacacccg ctcgcgcgcg atcacgacgg
    20461 cgaacgcggc gacgttcatc acgaggtagg cggcgaggta gaaggcggtc gcctgcaggc
    20521 cgagcttggt cgcgacgacg acgccggcga gcatgtaccc ggcctgcgcg acgccggacc
    20581 aggcgagcag ccgcttcagc gagcgctgca cgatcgcacc ggcgttcccg atcaggatcg
    20641 tcgcgactgc gagcgcggcg agcgcgggcg cccagtcgtc gtggaagtcg aacagcgcgc
    20701 cgccgaacag gcgcagcagg acagcgaagg cggcggcctt ggtggcgacc gccatgaacg
    20761 tcgtcaccgg ggtcggcgcg ccctggtaga cgtcgggcgt ccactggtgg aacggcgcga
    20821 ccgacgcctt gaacgcgagc ccgaccgccg ccagcgcgat gccggtcatc agcagcgggt
    20881 cgccgatccc cagccgaccg tcgagcgcgc gcgcgatccc gtcgaagtcg gtcgagccgc
    20941 tcgcgccgta cacgaacgcg aggccgtaga gcagcgtcgc cgacccgacc gagccgataa
    21001 cgaggtactt cagccccgac tcgagcgagt ggcggtcgcg cacagacgac gcgcagagca
    21061 cgtagagcgg gatcgacagc agctcgagac cgacgaagag cgtgacgagg ttctcggcgc
    21121 ccgccagcag cgtcatgccg gcgatcgacc cgagcatcat cgagtagtac tcgccggcgc
    21181 cggcctcctg caccgactcc cagcgcagcg agagcgcgat cgccgccagc ccggcgacgt
    21241 agaagagcag cgacatgaag agggcgagcg tgtcgatcgc cagcgcgttc tcgacgatcg
    21301 gccggctgtc gccgggctcc cagttgacga ccgagaagac gatcgcgacc accagcgcgg
    21361 cggcggcgag cagggggacc agcagccgct ggacgccgcg gccctcgaag aggctcgcga
    21421 gcaggacgac gaccgacccg ccggcgaccg cgagcaccgg cgcgaggccc tggtagtcga
    21481 tctcgggcga cggggtcgcg gcgagcaggt ccatcatcgc tagtgctcct cgccctggtc
    21541 ggacccggcg ggctcgccgg tcggctggcg ccgctggtcg gcgcgcgccg gcgtgccgtc
    21601 gccggttcgc tccgcggccg cctcgacctg cgcagtggtc gcctgctcgg tccgctcgag
    21661 tatcagctgc gggtagacgc cgagcgcgac gatcacggcg gcgatcggcg cgatcacgcc
    21721 gaggtcgagc cgcgacaggt cgccgcccac gaggtccggg ttctcgcggt tgtgcatcga
    21781 gcgttggaac aggcggatcg cgtagaccgc cgccagcgcg acgccgatgc tcgctaccag
    21841 cccgaagacg atcttgtcct cgagcgcgcc gaagaggatc aggatctcgc cgacgaagtt
    21901 cggcgagccg ggcatcgcaa gcgtcgcgag gatcacgatc aggaacagcg tcgcgagcac
    21961 cggcgcgcgc agcgcgatcc cgcccatctg ctcgagcgac ggcgagccgg ccgcgcgggc
    22021 ggtcagcacc gcgatcacga acagcagcgc gccgacgacc agcccgtggt tgaccatctg
    22081 gatcacagcg ccctgggcac cgaggtcgtc gatcgcgaag atcccgagcg tgatgaagcc
    22141 gagctgggcg atcgacgagt aggcgagcac cagccgtggc tcatcctgcg agaaggcgag
    22201 caccgagccg tagaggatcg agaagaccgc gatcaccagc agcaaatcct ggaagtggac
    22261 cgtcgcgtcg ggcatcacgg gcagcacgat ccgcaggaac gcgtagacgc cgaccttcga
    22321 gagcaccgcg gacagcacga cgagcaccgg cgtcggcgtc gcgcggtagg tgtcgacgac
    22381 ccagccgtgg aacgggaacg ccggcgcctt gacgaggaat gcgagcgcga agaggaggaa
    22441 gatccactgc tgcgagccct ccggcagcgg gttggccgcc agcgcggcga tcgagaaggt
    22501 caggtcgttg ccggtctccg aggcggtcag caccccaagc gccaccgcgc cggccagcat
    22561 caacagcgag ccgacgagcg tatagatgac gaacttcgtc gtagccgcga cgcggtcgcc
    22621 ctggccccag ccgccgatca ggaagtagaa cggcaccagc atcaggtcga agaagaggac
    22681 gaacagcgcg aggtcctggg cgaggaaggc gccaagcacc gccgtctcgg cgagcgccat
    22741 gtggaagagg aacagcttct ggcggtcggt ctcgcgcacg gccgaccacc acatcgccgg
    22801 cacccagagc agcgcggtca gcgcgacgag gaagaggttg agtccgtcga cgccgagctg
    22861 gtagcggata ccgagctcgg cgatccactc gtcgtcggtg acgtacttca tgccggcggg
    22921 cccgccgtcg aagtcggcga tcatcgcgat cgcgtagccg agtacggcga cgctgcccgc
    22981 gacgacgacc cagcggccga tgcgcgccgg cagcagcgcg ccgacgagcg cggccacgac
    23041 cggcaggaag accaggatgc tgaggtggac cgtcagcatc agctcgccgc gatcaggaag
    23101 tagaggacga tcgcgctgac gccgaccagc agcagcgcgg cgtaggcgcg cagctcgccg
    23161 gtctggatcg agcgcgcgaa cgagctgccc gcgcgcacga cgccgacggt gccgccgacg
    23221 aggaagccct ggacgaacgc gctctcgatc accgagcggc cgaagcgccc ggcggcgccc
    23281 gtcggccgca cgatcgccag gtcgtagagc tcgtcgaagt accacttgtt ggcgagcagc
    23341 cggtgcacgc cggccaagcg tgcctgcagc tgcgcggagg tccccgggcg ccagaggtag
    23401 attcgcgccg cgaccaggat gccggcgatc gacagcgccg cgccgaccgc catgccgagg
    23461 tactcggccg agtcgctcgg gtgctcgtgg acgtagtgcg agctcgcgaa cgtcggctcg
    23521 aggaacttct ccagcacgtc ggtcacgccc gggatctgga tgaagccggc gacgatcgcc
    23581 agcagcgcca gcggccccat cgcggccttc atcgcaccct cgtgctcggc gatgtggtgc
    23641 tcgggcccgg ggaagccggg gccggtgtcc tcgggctcgc cggtggccgg gttcgtgggc
    23701 gccgcgtgcg ccaggtggcc gccctcgagc tcgcgcgcct cggggctcgg ctccccgtgg
    23761 aagacgcgga acaccaaccg gaaggcgtag atcgcggtca ggaacgcggt caggtagccg
    23821 acgaccgcca gcgcgacgaa ccagtcgccg cggtgcagcg tgtaggccag gatctcgtcc
    23881 ttggagaaga agccggcggt cagcgggaag cccgccagcg cgagcgcgcc ggcggtgaag
    23941 gtgacgtagg tgaacggcat cgacttgcgg aagccgccca tcctgtccag cgactggttg
    24001 ctcgccatcg cggcgatcac cgagccggcg cccatgaaca gcagcgcctt gaagaaggcg
    24061 tgggtcatca ggtggaacag cccggcgccg taggcgaaga ccgagacgcc gaggatcatg
    24121 tagccgatct gcgacatcgt cgagtaggcg atcgc
//
LOCUS       DATABV010000140         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1328884, whole genome shotgun sequence.
ACCESSION   DATABV010000140 DATABV010000000
VERSION     DATABV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328884"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VHF88_06055"
     CDS             complement(<1..1068)
                     /locus_tag="VHF88_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX2161370.1"
                     /translation="MSRFFKSALFPILIVVALAFFAQKLISPGEQKKDTSYSQFVTQV
                     ENDQVKKATLNTKNNRIDVEQRNGDKYETAYPDNTEPSLVNKLQAANVDVDVKAKAGS
                     GWITALTYILPFVIFLLFWLFIINQMQGGGSKVMSFGKSRAKRMSVDSPKITFRDVAG
                     VDEAVEELHEIKEFLENPKKFQALGARIPKGVLLYGPPGTGKTLLARAVAGEAGVPFF
                     SISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRHRGAGLGGGHDERE
                     QTLNQLLVEMDGFTMTDNIILIAATNRPDILDPALLRPGRFDRQIVVDRPDRKGRAKI
                     LEVHTRGKPLAKSIDLDNLAAQ"
     gene            complement(1208..1744)
                     /gene="hpt"
                     /locus_tag="VHF88_06060"
     CDS             complement(1208..1744)
                     /gene="hpt"
                     /locus_tag="VHF88_06060"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX2161371.1"
                     /translation="MVAPEIGEILVQRDELAHRVQALAEQISHDYAGRSLLLIGVLKG
                     AMFFLSDLMRQLEVDCEVDFMAVSSYGSSTSSSGVVRILKDLDGPIEGRDVLIVEDIV
                     DSGLTLSYLLRTLRARDPASVEVCALLTKPERRKVDLPIQYVGFEIPNRFVIGYGLDH
                     AERFRNLPYVAVLDGTAT"
BASE COUNT          284 a          564 c          644 g          333 t
ORIGIN      
        1 ctgggcggcg aggttgtcga gatcgatcga cttggcgagc ggcttgccgc gcgtgtggac
       61 ctcgaggatc ttcgcgcggc ccttgcggtc gggacggtcc acgacgatct ggcggtcgaa
      121 gcggcccggg cgcagcagcg ccgggtcgag gatgtcgggc cggttcgtcg cggcgatcag
      181 gatgatgttg tcggtcatcg tgaagccgtc catctcgacg agcagctggt tgagcgtctg
      241 ctcgcgctcg tcgtgaccgc cgccgaggcc ggcgccgcgg tggcggccga cggcgtcgat
      301 ctcgtccatg aagatgatgc aggggctgtt ctgcttcgcc tgctcgaaca ggtcgcgcac
      361 gcgcgaggcg ccgacgccga cgaacatctc gacgaagtcg gagccggaga tcgagaagaa
      421 cggcacgccc gcctcgccgg cgacggcgcg cgcgagcagc gtcttgccgg ttcccggcgg
      481 tccgtagagc agcacaccct tgggtatccg cgcgccgagt gcctggaact tcttcgggtt
      541 ctcgaggaac tccttgatct cgtgcagctc ctcgaccgcc tcgtcgacgc cggcgacgtc
      601 gcggaaggtg atctttggcg aatcgaccga catccgcttc gcgcgcgact tgccgaacga
      661 catcaccttc gatccgccgc cctgcatctg gttgatgatg aacagccaga agagcaggaa
      721 gatcacgaac ggcaggatgt aggtgagcgc ggtgatccag ccgctgcccg ccttcgcctt
      781 gacgtctacg tcgacgttgg ccgcctgcag cttgttgacg aggctcggct cggtgttgtc
      841 cgggtaggcc gtctcgtact tgtcgccgtt gcgctgctcc acgtcgatgc ggttgttctt
      901 cgtgttgagc gtcgccttct tgacctggtc gttctcgacc tgggtcacga attggctgta
      961 gctggtgtcc ttcttctgct cgccggggct gatcagcttc tgcgcgaaga acgcgagggc
     1021 cacgacgatc aggatcggga acagtgcgct cttgaagaat cggctcacag gaggtctctc
     1081 tatcggcggc gacccgcgcg gactggatgc gacgggtaga tgccgaaggt acgccttagc
     1141 gggtgaaacg acagggtagc aagggtgtag cgggcctccc gccgcttggc gcctgtgttt
     1201 cgcgcgctca ggtggcggtc ccgtcgagga ctgccacgta gggcaggttg cggaaccgct
     1261 cggcgtggtc gaggccgtag ccgatcacga agcggttcgg tatctcgaag ccgacgtact
     1321 ggatcggcag gtccaccttg cgccgctccg gcttggtcag cagcgcgcaa acctcgaccg
     1381 aagcagggtc gcgtgcgcgc agggtgcgca acaggtagct gagcgtcagg ccggagtcga
     1441 cgatgtcctc gacgatcagc acgtcgcgtc cctcgatcgg accgtcgagg tccttgagga
     1501 tccgcacgac gccgctcgag ctggtcgagg agccatacga cgagacggcc atgaagtcga
     1561 cctcgcagtc gacctcgagc tggcgcatca ggtcgctgag gaagaacatc gcgcccttga
     1621 ggacgccgat cagcagcagc gagcggccgg cgtagtcgtg cgatatctgc tcggcgagcg
     1681 cctgcacgcg gtgggcgagc tcgtcgcgct ggacgaggat ctcaccgatc tcgggggcga
     1741 ccacggcggc gagtctacgg tgcgtggtgg tacgccggac cgacgggcat ccccgttccg
     1801 cttgggaatc gctgctcggg gatgc
//
LOCUS       DATABV010000141         4472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2131753, whole genome shotgun sequence.
ACCESSION   DATABV010000141 DATABV010000000
VERSION     DATABV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131753"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1313)
                     /gene="moeB"
                     /locus_tag="VHF88_06065"
     CDS             complement(123..1313)
                     /gene="moeB"
                     /locus_tag="VHF88_06065"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX2161372.1"
                     /translation="MPSGAELIKKAKEQITEVDPREVHDLLGNGHRNGTVLVDVREQH
                     EFEEAHLPGAVHVPRGHLESRIEGAAGDKSSRVILYCASGNRSALAAKTLAEELGYER
                     AESMTGGITLWKDRGYDVEVPRRLTAEQRERYSRHLLVPEIGLEGQLKLLDAKVLLLG
                     AGGLGAPAAFYLAAAGVGTLGIVDDDVVDLSNLQRQIVHTVDRVGMPKVDSAEQAIHE
                     LNPGVEVVKYKTRLDASNIMEIVEGYDVIVDGVDNFPTRYLLNDATVRLKIPVVSASI
                     LGFDGQLSVFAPYEGPCYRCLYPTPPPAELAPSCGANGVLGALPGIMGVLQAIEVVKL
                     ITGSGDPMIGRLLLYEALSTSFTDLKLRRDPECPICSRDPDSISDEEMGVFPDYEQFC
                     AAAG"
     gene            1585..2568
                     /locus_tag="VHF88_06070"
     CDS             1585..2568
                     /locus_tag="VHF88_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2161373.1"
                     /translation="MPRPLIRRRDDSLIGQVIAANVVLVTLTLLAASLAAGLDISIAE
                     QRWQFLILALAIVLTLCVNLWMLQKRFDPLERLVERLEAIDPAEPATFDIGDDPTAEI
                     DRLAGSFRRLLQRVDEERRRSGKLVLRGQEEERRRVARDLHDEVNQALTGILLRLEAL
                     AQDVPSDQRAQVTELKQLTGQAMDELLRLARQLRPTALDDHGLAPAIDAQARSFATRT
                     GVDASFAVSGDPSQLDEETQTVVYRVTQEALSNAAQHARPGRIEIELDAGEDGAVLLR
                     IRDDGEGFDPLASRAGGLGLDGMAERARLAGGALDLRSERGAGTELTLRLP"
     gene            2565..3242
                     /locus_tag="VHF88_06075"
     CDS             2565..3242
                     /locus_tag="VHF88_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2161374.1"
                     /translation="MTGAGAGPAGRARSSSRVLIADDHGVVRSGLAMLIERQADMSVV
                     AEAADGIAAVELALAQRPNVAVLDVSMPGLTGPQAARQIHAHDAGIGLLMLSMHEDER
                     YFFDALDAGALGYVPKRAADSDLIDAIRAVAAGRTFVSSSTQQALMRRWLEDDPGTRR
                     EELTARELEVVKLIAEARTNRQIAEALKVSEKTVESHRGNVLAKLGMRDRVELARYAI
                     RRGLVEP"
     gene            3347..3946
                     /locus_tag="VHF88_06080"
     CDS             3347..3946
                     /locus_tag="VHF88_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX2161375.1"
                     /translation="MNPRIVLALVVVAVAAFAAVALAAGSASDDAVEGGERFQGATLP
                     EGLRAPELGLRDEEGEVVRMAELRGEPVIVTFLYSNCDDSCPAQAQQVKGALNELGRD
                     LPALAISVDPANDTAQSARAFLSEQRMTGRMRFVLGDRPELQRVWRGYGVQPQLDDLE
                     HTARIVLVDAAGVQRVGYPLGEVTPERLAHDLRLLAAGR"
     gene            4029..>4472
                     /locus_tag="VHF88_06085"
     CDS             4029..>4472
                     /locus_tag="VHF88_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2161376.1"
                     /translation="MSLEIQNLHVRIEEREILRGLNLTVRKGETHALMGPNGSGKSTL
                     ANTIMGNPAYEVTDGRIVFNGEDMTEADPDERARAGLFMAFQYPATIPGVSVANFLRM
                     AVNAKREEPIKVKEFGKLLGENMELLRIDRAFTSRYLNEGFSGGEK"
BASE COUNT          684 a         1556 c         1597 g          635 t
ORIGIN      
        1 gtcgcgagcg cgcgcagcag ctctccgacg tcggcgccgt cggcggcgac ctcgcgctcg
       61 ccgccggtcg acgcgcgcag gaccggtggg atcttgatcg tgctcacggc gctgacccta
      121 cctcagccgg ctgcggcgca gaactgctcg tagtcgggga acacgcccat ctcctcgtcg
      181 gagatggagt cggggtcgcg cgagcagatc gggcactcgg gatcgcgacg cagcttgagg
      241 tcggtgaagc tcgtgctgag cgcctcgtag aggagcaggc ggccgatcat cgggtcgccg
      301 gagccggtga tgagcttgac gacctcgatc gcctgcagaa cgcccatgat cccgggcagc
      361 gcgccgagca cgccgttggc gccgcaggac ggcgccagct cggccggcgg cggcgtcggg
      421 tagaggcagc ggtagcaggg accctcgtac ggggcgaaga ccgacagctg gccgtcgaag
      481 ccgaggatcg aggcggacac gacggggatc ttcagccgca cggtcgcatc gttgagcagg
      541 tagcgcgtcg ggaagttgtc gacgccgtcg acgatcacgt cgtagccctc gacgatctcc
      601 atgatgttcg acgcgtcgag acgggtcttg tacttcacga cctcgacgcc ggggttgagc
      661 tcgtggatcg cctgctcggc ggagtcgacc ttcggcatgc cgacgcggtc gacggtgtgg
      721 actatctggc gctggaggtt cgacaggtcg acgacgtcgt cgtcgacgat gccgagcgtg
      781 ccgacgccgg cggcggcgag gtagaaggcc gccggcgcgc cgagcccgcc ggcgccgagc
      841 agcagcacct tcgcgtcgag cagcttgagc tgaccctcga ggccgatctc gggcacgagc
      901 aggtggcgcg agtagcgctc gcgctgctcg gccgtcagcc ggcgcgggac ctcgacgtcg
      961 tagccgcggt ccttccagag cgtgatgccg ccggtcatcg actcggcgcg ctcgtagccg
     1021 agctcctcag cgagcgtctt ggcggcgagc gcggagcggt tgccggaggc gcagtagagg
     1081 atcacccgcg acgacttgtc gccggcggcg ccctcgatgc gcgactcgag gtgcccgcgc
     1141 ggcacgtgga cggcgccggg caggtgggcc tcctcgaact cgtgctgctc gcggacgtcg
     1201 acgaggacgg tgccgttgcg gtgcccgttg cccagcaggt cgtggacctc gcgcgggtcg
     1261 acctcggtga tctgctcctt ggccttcttg atcagctcag caccgctggg catgcgaaaa
     1321 ctcctgaaag tcggtctggt ctaggtcaat tctacgcaag agctcgaagt tcgctctcca
     1381 cgccaatgct tcaaaaagga tagcgatggc gtctcagtcc caaaacgcaa ccggggcgcc
     1441 ctgttggacg ccccggctcg tacttcaccc agacactgct tgcggaaagc tcacggccca
     1501 gttgccgaaa gcctctgtcc acattctcgt catcggcacc cgctgccgat atggggtctg
     1561 gaccccatct tcgccgtccg ccctatgccg agaccactga tccgacgccg cgacgactcg
     1621 ctgatcggcc aggtcatcgc cgccaacgtc gtcctggtca cgctgacgct gctggcggcg
     1681 agcctcgcgg ccggcctcga catctcgatc gccgagcagc gctggcagtt cctgatcctc
     1741 gcgctggcga tcgtgctgac gctctgcgtc aacctctgga tgctgcagaa gcggttcgac
     1801 ccgctcgagc ggctcgtcga gcggctcgag gcgatcgatc cggctgagcc ggcgaccttc
     1861 gacatcggcg acgacccgac cgcggagatc gaccgcctcg caggctcgtt tcgccgactg
     1921 ctgcaacgcg tcgacgagga gcgccgccgc tccggcaagc tggtgctacg cggacaggag
     1981 gaggagcgcc ggcgcgtcgc gcgcgacctc cacgacgagg tcaaccaggc gctgaccggg
     2041 attctcctgc ggctcgaggc gctggcccag gacgtgccgt ccgatcagcg cgcccaggtg
     2101 accgagctca agcagctcac cgggcaggcg atggacgagc tgctgcgcct cgcgcgccag
     2161 ctgcgaccga cggcgctcga cgaccacggc ctggcgccgg cgatcgacgc ccaggcgcgc
     2221 agcttcgcca cccggaccgg cgtcgatgcc agcttcgccg tcagcggcga tccgagccag
     2281 ctcgacgagg agacccagac cgtcgtctac cgcgtcaccc aggaggcgct gtcgaacgcc
     2341 gcgcagcatg cccggccggg acggatcgag atcgagctcg acgccggcga ggatggcgcc
     2401 gtgctgctgc ggatccgcga cgacggcgag ggcttcgacc cgctcgccag ccgcgccggc
     2461 ggactcgggc tcgacggcat ggccgagcgg gcgcgcctcg cgggcggcgc gctcgacctg
     2521 cgctccgagc gcggcgccgg caccgagctc accttgaggc tgccgtgacc ggcgctggcg
     2581 ccggccctgc cggacgcgca cgatcgagtt cccgcgtcct gatcgccgac gaccacggcg
     2641 tggtgcgctc cggtctggcg atgctgatcg agcgccaggc cgacatgtcg gtcgtcgccg
     2701 aggccgccga cgggatcgcc gcagtcgagc ttgccctcgc gcagcgaccg aacgtcgcgg
     2761 tgctcgacgt ctcgatgccg ggcctgacag gcccccaggc agcgcggcag atccacgccc
     2821 acgacgccgg gatcggcctg ctgatgctct cgatgcacga ggacgagcgc tacttcttcg
     2881 acgcgctcga cgccggggcg ctcggctacg tgcccaagcg ggccgccgac tccgacctga
     2941 tcgacgcgat ccgggcggtc gccgctggac gcaccttcgt ctcctcctcg acgcagcagg
     3001 cgctgatgcg gcgctggctc gaagacgacc ccggcacccg ccgcgaggag ctcaccgcgc
     3061 gcgagctcga ggtcgtcaag ctgatcgccg aggcgcgcac gaaccgccag atcgccgagg
     3121 cgttgaaggt ctcggagaag accgtcgagt cgcaccgcgg caacgtcctc gccaagctcg
     3181 gcatgcgcga ccgcgtcgag ctcgcgcgct acgccatccg tagagggctt gtcgagccgt
     3241 gagccatagc cgcggtgctc accggacccc gctcccgcgt gggcgcgaga cgtcgccgta
     3301 ggaccggtcc gcctgcgctc ctccgcttga ccgtaccctg ggggtcgtga acccgaggat
     3361 cgtcctggcg ctggtggtgg tcgccgtggc ggcgtttgcg gccgttgcgc tggcggccgg
     3421 aagcgcttcg gacgacgcgg tcgagggcgg cgagcggttc cagggcgcga cactgccgga
     3481 ggggctgcgg gcgccggagc tggggctgcg cgacgaggag ggcgaggtcg tgcggatggc
     3541 ggagctgcgc ggcgagccgg tgatcgtgac cttcctctac tcgaactgcg acgacagctg
     3601 tccggcgcag gcgcagcagg tcaagggcgc gctgaacgag ctcgggcgcg acctgccggc
     3661 gctggcgatc tcggtcgacc cggcgaacga cacggcgcag agcgcgcggg cgttcctctc
     3721 ggagcagcgg atgaccgggc ggatgcgctt cgtgctcggc gaccgaccgg agctgcagcg
     3781 ggtgtggcgc ggctatggcg tccagccaca gctcgacgac ctcgagcaca cagcgcgtat
     3841 cgtgttggtc gacgccgccg gcgtccagcg ggtcggctac ccgctcggcg aggtgactcc
     3901 cgagcgtttg gcccacgatc tgcgactgct ggcagccggg cgctgagcgc gagcgccgcg
     3961 ccgataacct caagggccgc ccccaaggcg gccgaccacg acgaagaagg cacggagaac
     4021 gccaccgcat gagcttggag attcagaacc tgcacgtacg catcgaggag cgcgagatcc
     4081 tgcgcgggct gaacctgacc gtgcgcaagg gcgagacgca cgccctgatg gggccaaacg
     4141 gctcgggcaa gtcgacgctc gccaacacga tcatgggcaa cccggcctac gaggtcaccg
     4201 acggcaggat cgtcttcaac ggcgaggaca tgaccgaggc cgaccccgac gagcgggcgc
     4261 gcgccggcct cttcatggcg ttccagtacc cggcgacgat cccgggcgtc tcggtcgcga
     4321 acttcctgcg gatggccgtc aacgccaagc gcgaggagcc gatcaaggtc aaggagttcg
     4381 gcaagctgct cggcgagaac atggagctgc tgcggatcga ccgcgccttc accagccgct
     4441 acctcaacga gggcttctcc ggcggcgaga ag
//
LOCUS       DATABV010000142        33510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2212769, whole genome shotgun sequence.
ACCESSION   DATABV010000142 DATABV010000000
VERSION     DATABV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 33510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33510
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212769"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..136
                     /locus_tag="VHF88_06090"
     CDS             5..136
                     /locus_tag="VHF88_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX2161377.1"
                     /translation="MYDEQAGHPREGFPPGTPWSEVPDDWGCPDCGVRDKLDFEPEG"
     gene            142..771
                     /locus_tag="VHF88_06095"
     CDS             142..771
                     /locus_tag="VHF88_06095"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2161378.1"
                     /translation="MAAVATPERTPYAVAARELLRDTLLDAARDLLGERDWAEVTMAD
                     IATAGGVSRQTLYSEFGSRAEFAQALFLREADRFLGAVEDAVNVNLDDPVAALGAAFD
                     VFLRTAAENPLIRAVVAGEGNDSLLPYVTTQGQPVVEQAADRLAAVISAGWPHVDRDA
                     IDLLADCGVRLAISYAALPTGPADTTAASVATLLGPYLERVVAEAEGAS"
     gene            complement(805..876)
                     /locus_tag="VHF88_06100"
     tRNA            complement(805..876)
                     /locus_tag="VHF88_06100"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(841..843),aa:Trp,seq:cca)
     gene            1127..1903
                     /locus_tag="VHF88_06105"
     CDS             1127..1903
                     /locus_tag="VHF88_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161379.1"
                     /translation="MRTIVEPTAMYGGRDVRLMERVARETDLQVIACTGIYTYDFLPH
                     YWLTRDEDAMADAFVHDIENGIQRTPIKAAFLKCAADAAGVTPNVEKVHRACARASLR
                     TGAPIMAHTHPASDTGPRQMEIFVEEGVDPAKVQLAHTGDTDDLDYIERVLDTGAWIG
                     MDRYGIEMYLPTERRNATVLTLLERDYADRMFLSADSCASIDWFSPEAAEQLLEAGAV
                     KDWNTTLIHDKVIPALREGGMTDAQLQTMLEDNPRRWLAR"
     gene            1968..2038
                     /locus_tag="VHF88_06110"
     tRNA            1968..2038
                     /locus_tag="VHF88_06110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2000..2002,aa:Gly,seq:tcc)
     gene            complement(2056..2253)
                     /locus_tag="VHF88_06115"
     CDS             complement(2056..2253)
                     /locus_tag="VHF88_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161380.1"
                     /translation="MALKGRRGGKKVLGVPVPKPGNVDVEGIVKTVGKATKQFAATSK
                     SVSKDIERAGDKAERIGKILD"
     gene            2393..2923
                     /locus_tag="VHF88_06120"
     CDS             2393..2923
                     /locus_tag="VHF88_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161381.1"
                     /translation="MAFGFAGRFGLDRAAVVFLAAGLDLAAVVDFGFVVDFGFVAVFR
                     FAVDFGFAVVLGLEAAFDVAVDFDFAAAFGFDLAAVDFGAEPFDDLAPVRVRRLLCPD
                     LLVAILVPSDRSGPPRFDLPLEPLLQALPGSWFTVPSNSWRGRDQDDDASVGWPARPY
                     GDRCPAALGGGSGPAG"
     gene            2898..3866
                     /locus_tag="VHF88_06125"
     CDS             2898..3866
                     /locus_tag="VHF88_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HEX2161382.1"
                     /translation="MAVLALPVDARANQNPGAQAPNPLVGQEWWNQKTRWNPTWNGYR
                     SLLRRDRRADAAKVLALARTPQFKWFGMWEQPPVAKLRGLFENAGDKVPLLAIFGHDN
                     SGCGGGFTGGGAAEDARYRRWIGKVVRGIGPNEVVIAFEPDSLGTVECLVPSRRDDRL
                     RTLAYGVRRLSRLPNATVYVEAGASDWMSPRLAASRLRAVGVGRVRGFMLNATHMTTT
                     AANIRHGLAISRLVGGKHFIVNTSHNGNGPLYRNGHTIWCNPPNAAAGARPTTRTAHP
                     KVDAYMWVERPGYSNGSCNGGPLPVGAWWERKAIQLVERAKWWRRR"
     gene            3932..4624
                     /locus_tag="VHF88_06130"
     CDS             3932..4624
                     /locus_tag="VHF88_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161383.1"
                     /translation="MGGTLSQAFSIYGSQAGVLIPLALALFLVVAVVNGLAAESVVLL
                     PVGLAVSIIAGTLYTGMVVALVSDVQDGRRDYSVGELARSASHVILPLIGAGLLAGIG
                     IGIGFLLLVIPGLFLMTIWAVIAPVIVLERVGVMKSFGRSRELVKGDGWRVFGVIVIV
                     FIIVIVVRAILGAIAVGIDDSTVAQIIFDWIGSSLTAPISALVAAVIYFRLKGAKDGG
                     APVPAEPGPATV"
     gene            4629..5048
                     /locus_tag="VHF88_06135"
     CDS             4629..5048
                     /locus_tag="VHF88_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2161384.1"
                     /translation="MRQRVSLITLGVRDLERARAFYEALGWQTGAEPGDDIVFFQAGD
                     MIVALWDRARLAEDSCVEDAGGWGGTTLALTLGSPAEVDAAIEEARAAGATIGREPAD
                     TFWGGYSGLMIDPDGHPWEIAHNPHWTITEDGGVRLA"
     gene            5118..5327
                     /locus_tag="VHF88_06140"
     CDS             5118..5327
                     /locus_tag="VHF88_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161385.1"
                     /translation="MTAKEKLRAQVEELSEAEAAAARIVVERTPIDLDTEPEMVGLPE
                     LWRTFEDGTPVPNWVAGLDEVRAGR"
     gene            5327..5716
                     /locus_tag="VHF88_06145"
     CDS             5327..5716
                     /locus_tag="VHF88_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2161386.1"
                     /translation="MLVIDASAATAACARAEGFEALGDDLVAPPLMWSEVRANLHLQA
                     FKRSISYDRADVLHERLGAASIRCENPSDLGRNAWALATEMGWGRTYDAEYIALAQIL
                     GCRVVTVDGRLRRGAHRLGLVVTPDEL"
     gene            complement(5814..7607)
                     /locus_tag="VHF88_06150"
     CDS             complement(5814..7607)
                     /locus_tag="VHF88_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX2161387.1"
                     /translation="MTDAEVEISGPTAAVPKDPQPSVDRIDELARRILSGDILLPKFQ
                     RSFVWERPQILTLLDSVARGYPIGSVLLWQSRQELRSENRIADLEIDLPKPDYPVNYL
                     LDGQQRLSTICGALYWTVGEARSRWNIAYDLREKKFLHLDTADDPPLHQIRINKLADP
                     ATYFQHVASLDSLMADDKEDLKAHANVLFNRFKDYKIAAVTLGDMSLEDVAPIFERIN
                     SMGTPLTIVDLMRAATWSPDFDLIDSIEGILDRLQAKGFGTIDRKTVLRNLAAAIGGG
                     FSTDSIDALRNYDADELKAAVEEVSNAYKRMVDFLATDMRVPGGDVVPYSNQLTVLAE
                     VFRLLPTPSADQHAAIRAWFWRTALAGYFSGWNTGMMRSDLEAVQAFAKGDSAEISVG
                     ATEPSVSIWTTRQFRSNNAHAKLLAIVLSYHQPVDLLTGQQIDTTKALAWMNSKEFHH
                     FFPQAYLKLQKVPRLRINALANIVMLTSNSNKEILDSPPSEYLTRVEEAAGDALEAWL
                     ASNLISMEAFAAAKANDFDTFLSLRAQAIHSAVLQLTGWGETSEVPSIESAEAISDEV
                     SDDADSIEYVVEEPSTDLTHSVGADGADTTT"
     gene            complement(7765..7838)
                     /locus_tag="VHF88_06155"
     tRNA            complement(7765..7838)
                     /locus_tag="VHF88_06155"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7802..7804),aa:Pro,seq:ggg)
     gene            7910..8662
                     /locus_tag="VHF88_06160"
     CDS             7910..8662
                     /locus_tag="VHF88_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2161388.1"
                     /translation="MPDQPKVVLHAVPFSHPCLAVQAVLDRHGLEYEKVDLVSGPHAD
                     EIERIYGEGKRTVPGLLVDDEPVHGTTAIFEKLDELVDDADLYPAAVAAAIREAERGI
                     AEDLQTTARNLVFGALHFRPEALGTFAGTGPLDPAGTDFAIRMVRGAWRYIGISAELL
                     ATTLSELPEQLDIVDELIEGGVAGGDDPTAVDFQLGSSLHLLLQIGDVRPLIEGRPAG
                     ALVDRWFEPGPADIPAGAFPAGWVPERSQSAA"
     gene            complement(8647..9405)
                     /locus_tag="VHF88_06165"
     CDS             complement(8647..9405)
                     /locus_tag="VHF88_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2161389.1"
                     /translation="MDGDPLALTALLVWCFAVAVAGGTVGLVLGNIRLPAVVAVASSP
                     AAGAGANIGISGAAALAAAIVHVRGGRIDWRVFAWMTPPSVAGALIGGYAAGLLPDAV
                     LLAVIGTTLLYFGFDLLRRRERRSATEDQDGPDLVALALAGLGIGLLGGLIGLILGAL
                     RMPALLRWHGGSAHRAVGTNLSVGVCVGVAGVIGHTPAGVDWEVLALGAAASVPGALL
                     GARLTGRLSERALLRAIGAMLVVAGTAMLAQAAL"
     gene            complement(9412..10515)
                     /locus_tag="VHF88_06170"
     CDS             complement(9412..10515)
                     /locus_tag="VHF88_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX2161390.1"
                     /translation="MAILWPGDVDTRVGEAIVTKSDTATVLSVSEFACAGLETDVCRE
                     VRIRLESGAQSGATKELVLDDGTIDPEVSVGDELRVVRNVVPPGADPVPGREYSISDF
                     DRQEPLLLLALIFAGVVVLFGRLRGALSLIGLGLSLAVVLGFMVPAILDGKSTLAVAI
                     VGSVAVMLTTILLAHGANPKSLAAIVGTAASLVVTVGLAVLFTDLTHLTGLASEEATF
                     LQINDSGVSLEGLLLAGMIVAALGVLDDVTVSQSSTVLALRAANPGQSFRQLFKLAMD
                     VGRDHVSATVNTLVLAYVGASLPLLLIFSSGQIDVVDAVNLEIIAKEIVATLVGSIGL
                     IAAVPITTALAAMLARRMSADELAAVEPHGHTH"
     gene            10701..11012
                     /gene="rpsR"
                     /locus_tag="VHF88_06175"
     CDS             10701..11012
                     /gene="rpsR"
                     /locus_tag="VHF88_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX2161391.1"
                     /translation="MARGNTKDRKRGKPSLKGEARRYSKLPREEIEKIDYKNIALLQR
                     FVSERGKIRSRRVTGLSRRDQSKMARAVKRARELGLLPYVDASKAVERVGGGRGRRER
                     E"
     gene            complement(11022..11936)
                     /locus_tag="VHF88_06180"
     CDS             complement(11022..11936)
                     /locus_tag="VHF88_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2161392.1"
                     /translation="MAHDHSAHAHGIPREGDGRRLWIALGLILALMAGEVVAGIVAGS
                     LALLSDAAHMLTDAAAIALALFAARLAARPPRGGYTYGLRRAEILSAQANGATLLALA
                     AVIGFEAVRRIADPPSVEGGLVLVVALVGVAVNVLATWVLAGANRDSLNVEGAFQHIL
                     TDLFAFIATAVAGAVILTTGFGQADGIAALIVAALMTRAGWGLLRDSGRILLEGSPRG
                     IDPNQIAQALADEPDVVGVHDLHVWEVTSGFPALTAHVLLEPDCDCHQRRRDFEAMLH
                     DRFGIDHTTIQVDHAHAEQGPIELERGG"
     gene            12038..13024
                     /locus_tag="VHF88_06185"
     CDS             12038..13024
                     /locus_tag="VHF88_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX2161393.1"
                     /translation="MSRRRSTILVSIVLAAALAIGCGDDGGSAARGEKALTAVATTTQ
                     VADFAREVGGDRVDVRQILQPNSDPHGYEPRPSDVASVTEADVVMRSGGDLDAWLDGL
                     LDNAGADVPQVELIQAVERRDGGHAGEGSHDHVGEEEHDHGDSADPHWWQDPRNAIAA
                     VAAIRDALAAADPDGRAEYERNAAAYIRQLRRLDRAVAACIDEVPAPQRKLVTTHDAL
                     GYYADRYGIEVVGALIPSQSTAAQPSAGDIEELVDQIEREQVEAIFPESSIDPRLERA
                     VSRETGATVGKALWADTLGGEGSGGATYVESIRSNTAALVEGLTGGAQRCRP"
     gene            complement(13040..13492)
                     /locus_tag="VHF88_06190"
     CDS             complement(13040..13492)
                     /locus_tag="VHF88_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2161394.1"
                     /translation="MASTTASGWTEHTARTLADAGYRSSAAREAVIEAIAELGCSMTA
                     REIADLLRERGNGVGLASIYRALDLLDRLGLVQRFDVGEGVARYEAALPGGDHHHHLV
                     CESCGRVEPFEDELLEEAIHALSAKTDFAIAAHDVTLHGECPACRASA"
     gene            13526..14395
                     /locus_tag="VHF88_06195"
     CDS             13526..14395
                     /locus_tag="VHF88_06195"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX2161395.1"
                     /translation="MEIHRTPDERFSDLPGYPFEPHYAEVGGLRMHYVDEGEGSPVVC
                     FHGEPSWSYLYRKMLPPLVAAGHRVICPDLAGFGRSDKPIDRGWYSYDRHVEQVAGVL
                     DGLDLSGATVVVQDWGGPIGLRWAVENATRVDRLVILNTGLFTGRVSKGFLQWRSFAE
                     QNPDLPVGMVVQGATTTDLPPEVVTAYEAPFPDAESKAGAATFPLLVPIDESAPGASE
                     MAAVMDALSRWDKPALVAFSDSDPVFPSPKAGQVFVDLIPGAGEQLVIEGGGHFLQED
                     RGEVIAAAILNWE"
     gene            complement(14495..15100)
                     /locus_tag="VHF88_06200"
     CDS             complement(14495..15100)
                     /locus_tag="VHF88_06200"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX2161396.1"
                     /translation="MAYEVPDLPYDYAALEPHIDEATMRVHHDKHHQAYVDKANAALE
                     GTDWAGKPIEDVIANLSQLPDDIRGPVRNNGGGHLNHTLFWESMSPNGGGQPDGDLAA
                     AIDSAFGSFSDFQDKVKATGVGQFGSGWAWLVHDGSGLAVVGTPNQDNPLTDGKTPLL
                     GVDVWEHAYYLKYQNKRPDYIDAWWNVVDWSKVAERFSAVA"
     gene            complement(15149..16276)
                     /locus_tag="VHF88_06205"
     CDS             complement(15149..16276)
                     /locus_tag="VHF88_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX2161397.1"
                     /translation="MSDPSQGESAYASAGVDYATLDAGKRLALTEALGTSAALAAHGG
                     RALDVSRGEPAFVFELGDRTLAFVMEGLGTKAVASRLVAEQLGLNRFDAVAYDTVAAI
                     VNDLCCVGALPLVVNAYFATGSSDWYRNTEWHSALVKGWSRACADAGATWGGGESPSL
                     PGLVNGEDIELAGSAVGALPDGRQPILGQDLSPGDEIMIVASNGLHANGASLARMIAD
                     RLANGYATPLPSGRSYGEALLDPSILYARLVGALAEADVPVSYLSHVTGHGLLKLMRP
                     SRDLTYRITALPEVPEVLAFLAEQAEMDAHAAYSTLNMGSGFAVYSAAGSAERVVEIA
                     RGLGHEALVAGRVEEGPRQVIVEPIGVTYADAELDLAPDRA"
     gene            complement(16304..16387)
                     /locus_tag="VHF88_06210"
     tRNA            complement(16304..16387)
                     /locus_tag="VHF88_06210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16351..16353),aa:Leu,seq:cag)
     gene            complement(16419..17045)
                     /locus_tag="VHF88_06215"
     CDS             complement(16419..17045)
                     /locus_tag="VHF88_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX2161398.1"
                     /translation="MDERRSDVLKASPRMFESNLLDWLSRVHPVVPLVIFIPAIAVLF
                     ALGQEGTPPFEVTAWIAGGWVFWTLTEYWLHRLVFHLEPEDGIGARLHWIIHGVHHDH
                     PNDPMRLVMPPSVSVPLALAFWALFHWAIGGQSADLFACGFLVGYLAYDMTHYHLHHH
                     KPRTRLGKLFYELHMRHHFQDHTRGFGVAAPWWDAAFGTRLRRRKTRD"
     gene            complement(17100..17315)
                     /locus_tag="VHF88_06220"
     CDS             complement(17100..17315)
                     /locus_tag="VHF88_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161399.1"
                     /translation="MNSTTIETIHAYIRWAFMAAVTATLLAMTFEQARRGLPGLAIAS
                     AYALTAGFGLEFLRAAHPVRPRGENAS"
     gene            complement(17372..17722)
                     /locus_tag="VHF88_06225"
     CDS             complement(17372..17722)
                     /locus_tag="VHF88_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161400.1"
                     /translation="MRRPWSWNETRARTLAIYRSEEHDRERTAVLVWQGENGRVAVGD
                     ATPRLLVVTVDAEHEAGTDNAIAIAGFDSLEQARAHCVDWAGTTAETSWEALGEAAQV
                     GVFAAIREPGFTSL"
     gene            17806..18483
                     /locus_tag="VHF88_06230"
     CDS             17806..18483
                     /locus_tag="VHF88_06230"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161401.1"
                     /translation="MVCLMAVSSTSRTRLPRAERMEQTVAAAHALFAERGYAAVTMDG
                     VAAAVGVTKPLLYNYFGNKEQLYLACLERAGDGLVATVVEAVGETSNPTEALSLGLHA
                     FFGFVDQDRAAWAVLFDETLPAGGEIAEQVAGYRTRLRDLIAASMLEQLPPRSRERAR
                     TEVEALSTAIMGAAEALVRWWLRTGAMPAADVADLLISTVEPGLRQRSAPAKSSKPNS
                     PGGDPPR"
     gene            18480..19775
                     /locus_tag="VHF88_06235"
     CDS             18480..19775
                     /locus_tag="VHF88_06235"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX2161402.1"
                     /translation="MNSKTRPVAVVGGNRIPFARSNSAYSKASNQDMLTAALDGLIDR
                     FDLDGVELGEVAGGAVLKHSRDRDLTRESVLGTRLAPETPAYDLQQACGTGLEAAILV
                     ANKIALGQIDSGIACGADTTSDAPIALNEDLREVLLEANRQRSTTGKLKQLAKVRPGH
                     VVPAIPRNEEPRTGMSMGEHCAIMATEWGVGRAEQDELTVRSHENLAAAYERGFMDDL
                     MTPYLGLERDQNLRPGSSVEKLGKLKPVFGGEDGTMTAANSTPLSDGASAVLLASEDW
                     ARERGLPILARFADGQTAAVDHVHKREGLLMAPAYAMPRMLDRAGLTLQDFDFYEIHE
                     AFAAQVLSTLRAWEDPVFCKERLGRDAPLGAIDRDKLNVNGGSLAAGHPFAATGGRIV
                     ASLAKALHERGSGRGLISICAAGGQGVVAILEAPGGNSK"
     gene            19772..21169
                     /locus_tag="VHF88_06240"
     CDS             19772..21169
                     /locus_tag="VHF88_06240"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX2161403.1"
                     /translation="MSDRYSQIVNSFPGKLVAGRVGLPQPVELDRHRPGAPVVSGRVL
                     LGAAPGGRLGTAVARVLAAAGAETSTTPDPTLREAAAAASLDAALFDPESGGDQRFKA
                     LVFDATGISDSTELVELQRFFHPTVRKLERSGRVVVLGTPAFGCDTPRESTAQRALEG
                     FTRSLGKEVGRGTTVQLVYVSPGAEDELDSTLRFLLSPKSAYVSGQVVRVGPGVAKAP
                     KLDWDKPLAGKRALVTGASRGIGAAIAETLRRDGADLVLLDVPALSADLKQVAKRLDA
                     DTIELDITEEGAPAAIAEKLSDGVDVVVHNAGVTRDRTIAKMPEERWTGLMEINLSSE
                     ERIDDALLEANLLRPNGRIVCVSSMSGIAGNSGQTNYATSKAGVIGMVEALAPTLAEK
                     GLTINAVAPGFIETQMTGAMPIAIREAGRRMNSMSQGGLPVDVAETIAWFASPASTGV
                     NGNVVRVCGQSLLGA"
     gene            21169..22104
                     /locus_tag="VHF88_06245"
     CDS             21169..22104
                     /locus_tag="VHF88_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX2161404.1"
                     /translation="MRQRTLTGPPSILPLYARAAAPLIPGASLLPFVPGGGKEIPDVE
                     LTLPDVVADSEAVAAYAKVCGFTLRDSLPPTYPHVLAFPLHMALMADGSFPFGAVGLV
                     HVENRIVQHRPIGLAEQLTLRVRGTKLEPHPKGRTFSIVSEARVGAELVWEDVSTMLR
                     RGGGRSEPNGGKDAPGPADADNAEQAASAEWKLGGDLGRRYAAVSGDRNPIHMHSLTA
                     KPLGFPRAIAHGMWTKARALAALEGRLPDAFAVDVRFRRPILLPATVAFASSDSGDEI
                     DFAVRDAKRGTPHLDGRVAPLEAKKKTSTGRKRAS"
     gene            22101..23435
                     /locus_tag="VHF88_06250"
     CDS             22101..23435
                     /locus_tag="VHF88_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161405.1"
                     /translation="MNATTDKGMAERGMGFWLRALNRLAGSDLLDRIRMRKQVERALF
                     QGTKQGFRSATAASRSFKAAQKLGRPARQPHAKPRGLFDITPDDEQAMLQEAVGAFAA
                     EQVRPIALEADAACATPPELLTQSTELGVSMLGIPEELGGVMHEQSAVTSVLIGEALA
                     HGDMGVAYAALAPGAVATAIAHWGDADQEATYLPAFASENVPAAALTLLEPRPLFDPL
                     KLETTARKDGEDWVLDGVKSLVARAADCELFVVGAQAEGIGPALFVIESSTGGLSVES
                     EPAMGLRPAATGRLLLEGVRLPAGALLGDGEPGVYSECVDRARIAWCSLAVGTAQAVL
                     DYVIPYVNERTAFGEPISNRQAVAFAVSDIGIESDGMRLATYRAASRADHGSDFARET
                     AVARKLCADKGMKIGSDGVQLLGGHGYIKEHPVERWYRDLRAAGVMEGALLV"
     gene            23463..24674
                     /locus_tag="VHF88_06255"
     CDS             23463..24674
                     /locus_tag="VHF88_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161406.1"
                     /translation="MIDLEIPKKFRPLVSQANQVATEVFRPISRKYDVAEHEYPKELD
                     MLASLIDGMNEGSDLGGAGAAGVRREENGGEAKNRNGSNLSTVLGIIELCWGDVGLLL
                     SMPRQGLGNSAIASVATEEQLERYGGKWAAMAITEPEAGSDSASIRTTAELDGDEYVL
                     NGEKIYVTSGERADLVVVWASLDRSKGRAAIKSFIVERSNPGMKLDRLEHKLGIRASD
                     TAAFILDNCRVPKEALLGSPEVDVKAGFGGAMQTFDNTRPLVAAMAVGVARASLDETR
                     TRLAEAGVEVDYSRPTLAQSAAAARFLEMEADYEASRLLTLQAAWMADNGKPNSLQAS
                     MAKAKAGRTCVDVSLGCVELCGSLGYGENELLEKWARDAKILDIFEGTQQIQLLIVAR
                     QLLGKSSAELK"
     gene            complement(24843..25070)
                     /locus_tag="VHF88_06260"
     CDS             complement(24843..25070)
                     /locus_tag="VHF88_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2161407.1"
                     /translation="MDDPLPQFGSNLRKARLARGLSQERLAHLCGLNMTHVARIERAE
                     REPGIRTVSKLARGLDVRASVLFEGLEGRDA"
     gene            25190..25423
                     /locus_tag="VHF88_06265"
     CDS             25190..25423
                     /locus_tag="VHF88_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2161408.1"
                     /translation="MSRDVLVFFAENLRRRRKELGLSQEQLGARADIQMADISRYESG
                     SRDPRITTVARLADALEVSIGDLLQASADGRVS"
     gene            complement(25453..26082)
                     /locus_tag="VHF88_06270"
     CDS             complement(25453..26082)
                     /locus_tag="VHF88_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2161409.1"
                     /translation="MAVLREPRSTSGVSSFVGGVAEKAVGDAVHCVLVISYSMSVSAD
                     GYVAGPDGSIDWTAPSEELHRFHNERTAATDVHLLGRRLYETMLVWDVEEFSDLAMAE
                     FAEIWRPLEKVVFSTTLTSVEGSNRLATGTLDEEIAALGDRRIAVGGAGLAAACMRRG
                     LIDEFQLFVVPIVIGGGTPYFPPDVHVDLELVETRTFAGTVYLHYRRRG"
     gene            26343..26618
                     /locus_tag="VHF88_06275"
     CDS             26343..26618
                     /locus_tag="VHF88_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161410.1"
                     /translation="MNDDDSALERSVEEAMAQQELDAVRELLATTSGLFPHARTPDEM
                     QRLVDRLETEEGQRDELERIKRIVEATPQLREVFERVVSFQRFHERP"
     gene            26651..28762
                     /locus_tag="VHF88_06280"
     CDS             26651..28762
                     /locus_tag="VHF88_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HEX2161411.1"
                     /translation="MRFWPRRPTPVATGVSLDDDGVLLDADAALEDERDDGLDRVLFS
                     DRLAHQIKRARAKTGLVVALIGPWGSGKTTVLGWVQKRLEEGQPKDDPSRVVVRFNPW
                     LVSGSEQLVSQFVSELAVALPRRAGERRAADAAARLHRYARALQILEGAPGVGWMFGT
                     GGALLNEIGRRRHETPETVFEQREQVCKALEALGLHVVVVIDDVDRLEDTEIRDLMRM
                     VKLVGDFPNVTYLLAFDREPVLSALSVGQADGGQYLEKIVQIEYRLPEPRPEKLDLLL
                     RAGLKREIPGHVLESDRWETVYTKIVLPLVSRPRHVRRLANALPLELDQAGDEVNPID
                     VVALTALQVLLPKFYDELRQLTEDLTPRFTQFLGWTKDEIKQASAARLTQAAERSGNP
                     DVARTVYELLFPAAQAVQSDVYYSDEPSEWRADQRVAERDVLERFFTGTLPDDAVTVA
                     DVRAALEGFAEGPVALAAVFDYQPAEKIPALVQRVRAQAREIPAESIVPCLPVLDAQI
                     ARISDFTAGLSSPGDFVRFLIRDLVERFPEGSARDSVVEQRFEMAPSLTEKFTWLRSI
                     GAWEDGGGNFASPETSQKLRERLAGEVLEAGVETLLKEEHPAWLLRTAEDDGGSDASD
                     RVGTLIGDGRLLPAYLGGFLVLTTSADYQLQFDELERRQGRTRLVEAAEHVDLTSLKD
                     EERWRVLQLREHYDGRGPLDS"
     gene            28908..29135
                     /locus_tag="VHF88_06285"
     CDS             28908..29135
                     /locus_tag="VHF88_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161412.1"
                     /translation="MASPVAQDHRIDTAIVGLLIDSPTPWSVAEIEREIGDEIATVDA
                     LARLARQGLIHRLDDFVFISRSAACAFELAA"
     gene            complement(29660..30709)
                     /locus_tag="VHF88_06290"
     CDS             complement(29660..30709)
                     /locus_tag="VHF88_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2161413.1"
                     /translation="MRDGEILRRTFGSLAAAKKWRVQATNALEDGTLKGDVKTSVDDA
                     AREFIDGARSGLIRNRVGQPYKPASVRGYDRSLRLHVLPALGHLRLSHVRRRHVQDLV
                     DDLMANGLAGSTVKNACDPLRRIFDRALKRDLIAVDPTDGIEWPASSRCRDRIATPAE
                     AQRLLDALPEDDCALWATALYAGLRRGELRALRWSAVDFGKKVINVERGWDDVGGDQE
                     GKTRAARRRVPLVPDLRKLLRAHELATGRRGSDLVFGISETVPFEPSTLRRRALKAWR
                     DADFRPIGLHECRHTFASLMIAADVNIKALSTYMGHTSVTTTLDLYGHLLPNAEDEAA
                     DRLQSFLDRQRGETA"
     gene            31326..31499
                     /locus_tag="VHF88_06295"
     CDS             31326..31499
                     /locus_tag="VHF88_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161414.1"
                     /translation="MASVDHGELVPEATPVELLLLPLLGSGLAIGRHFLEKIERLERA
                     HHRSVVNTVECSS"
     gene            complement(31630..32118)
                     /locus_tag="VHF88_06300"
     CDS             complement(31630..32118)
                     /locus_tag="VHF88_06300"
                     /inference="COORDINATES: protein motif:HMM:NF042490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6650 family protein"
                     /protein_id="HEX2161415.1"
                     /translation="MKFSALLSRVNGFSTPVFGMSWEPARSDVEVAQGLLLFLEDRRV
                     LYSPYEVEVPEHCVESVQEIRRFLTDVLVKGGIADELAEPLSAMRASCRKFLDEVNKD
                     RVLRERWQLTDMMGVQGWTFNQALGQLRGVVGVHVAQVAVRYGLDVPDVLDPILPTVD
                     SD"
     gene            complement(32147..32743)
                     /locus_tag="VHF88_06305"
     CDS             complement(32147..32743)
                     /locus_tag="VHF88_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161416.1"
                     /translation="MDSAVITGLFTLGGVVVGGVLNQALNHHLERRRAGWEAKKAARL
                     FAPSLHRLHIAGEHGRTHAVTWDEMADLAKHELVGWETHADVFAGTIEWDDWFEIYSA
                     VRVWQQIAEDPPRNEHGGLIRPGTPDAKRLAEITEKALDGALQCGSIGVNGIAKRRFR
                     RTAAKLRDRLPGKERREKRFESLIRKDFEERFGFPLDS"
     gene            complement(32853..32925)
                     /locus_tag="VHF88_06310"
     tRNA            complement(32853..32925)
                     /locus_tag="VHF88_06310"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32889..32891),aa:Glu,seq:ctc)
     gene            complement(33024..>33510)
                     /locus_tag="VHF88_06315"
     CDS             complement(33024..>33510)
                     /locus_tag="VHF88_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161417.1"
                     /translation="HQVAVDTAKTVARVPARDGTTYEFRVAAIDRAGNFSPYATGTST
                     VPVDNLANRVRFSKDGWKTLRRHGAFKLSVARATRKGASASLRFTGTGATIVTRKLPK
                     GGRLRVTLDGESKVVRLKGRGRFRRTLVETPSVAAGQHRLRVTSLGRAPVEIDAIAVK
                     P"
BASE COUNT         5244 a        11454 c        11636 g         5176 t
ORIGIN      
        1 ctacgtgtac gacgagcagg cgggtcatcc gcgcgagggc ttcccgcccg gcacgccctg
       61 gagtgaggtg cccgacgact ggggctgccc cgactgcggc gtgcgcgaca agctcgactt
      121 cgagccggag ggttgaccaa gatggctgcg gtggccacac ccgagcgaac cccgtatgcc
      181 gtcgcggcgc gcgagctgtt gcgcgacacg ctgctcgacg cggcgcgcga cctgctcggc
      241 gagcgcgact gggccgaggt cacgatggcc gacatcgcca ccgccggcgg cgtcagccgc
      301 cagacgctct acagcgagtt cggctcacgc gcggagttcg cccaggccct cttcctgcgc
      361 gaggccgacc gcttcctcgg cgcggtcgag gacgccgtca acgtcaacct cgacgatccg
      421 gtcgccgccc tcggcgccgc gttcgacgtc ttcctgagga ccgccgccga gaacccgctg
      481 atccgggcag tcgtcgcggg cgagggcaac gacagcctgc tgccctacgt caccacccag
      541 ggccagcccg tcgtcgagca ggccgccgac cgactcgcgg cggtcatctc cgccggctgg
      601 ccgcacgtcg accgcgacgc catcgacctg ctcgccgatt gcggcgtccg cctcgcgatc
      661 agctacgccg ccctgccgac cggaccggcg gacaccaccg ccgcgtcagt cgccaccctg
      721 ctcggtccct acctcgagcg ggtggtcgcc gaggcggagg gtgcctcgta ggcgtcacga
      781 cagcggggag atcgacgatc ccagcggggg agccgggatt cgaacccgga ttcacggttt
      841 tggagaccgt gcgcatagcc gttaactcac tcccccgaga gcgagcgatt ctagggagcc
      901 gacagtagga tcgccggcga gggggtcgat gcaggggaga ggagaggcgt ggggactgtc
      961 gagagcgtga cggggccgtt ggagctggac gagctggggg tcacgttgat ccacgagcac
     1021 ctctgcttcc gcgacgaggc gacggcgatc cagtggccgc acctctacga cgaggacgcg
     1081 ctccaccagg cggcgggagg cggcgaccgg ggcgctcggg gtgggcgtgc ggacgatcgt
     1141 cgagccgacg gcgatgtacg gcggccggga cgtccgcctg atggaacggg tggcgcgcga
     1201 gaccgacctg caggtgatcg cctgcaccgg gatctacacc tacgacttcc ttccccatta
     1261 ctggctgacg cgcgacgagg atgcgatggc cgacgcgttc gtccatgaca tcgagaacgg
     1321 gatccagcgg acgccgatca aggcggcgtt cctcaagtgc gcggccgacg cggccggcgt
     1381 cacgcctaac gtcgagaagg tccaccgcgc ctgcgcgcgc gccagcctgc gtaccggcgc
     1441 gccgatcatg gcccacaccc acccggcgtc ggacaccggg ccgcggcaga tggagatctt
     1501 cgtcgaggag ggcgtcgatc cggcgaaggt ccagctcgcg cacaccggcg acaccgacga
     1561 cctcgactac atcgagcggg tgctcgacac gggggcctgg atcggcatgg accgctacgg
     1621 gatcgagatg tacctgccga ccgagcgccg caatgccacg gtcctgacgc tgctggagcg
     1681 cgactacgcc gaccgtatgt tcctgtcggc cgactcctgc gcctcgatcg actggttctc
     1741 gcctgaggcg gccgagcagc tgctcgaggc cggcgcggtc aaggactgga acaccacgct
     1801 gattcacgac aaggtgatcc cggcgctgcg cgagggcgga atgaccgacg cccagctgca
     1861 gacgatgctc gaggacaacc cgcgccgctg gctggcccgc tgaaggcggg cgtgcgcggc
     1921 gcgtgacaga tcgccgcgac ggccttacaa tccgcgcttc ggtgcccgcg ggtgtggtgt
     1981 aagggctgca caagagcctt ccaagctctt agtacgggtt cgaatcccgt cgcccgcttc
     2041 ccgtcagccc tcgcgctagt cgaggatctt cccgatccgc tcggccttgt cgccggcgcg
     2101 ctcgatgtcc ttagagaccg acttcgaggt cgcggcgaac tgcttagtcg ccttgccgac
     2161 ggtcttgacg atcccctcga cgtcgacgtt gcccggcttg ggcaccggga cgccgagcac
     2221 cttcttgccg ccccggcggc ccttcagcgc catgccgccg gcgacgccta ccgctgcggc
     2281 tccgaccgcc agcgctgggc gcgcagcctt gccggcaact tgcttggcct tgccggcagc
     2341 ctgcttcgtg gtgtcggttg cctgatctac gagaccggtg gacgcctgag ccgtggcctt
     2401 cggcttcgcg gggcgcttcg gcctcgaccg cgcggctgtg gtcttcttgg ccgctggctt
     2461 ggacctcgcc gcggtcgtcg acttcggctt cgtggtcgac tttggcttcg tggccgtctt
     2521 ccgcttcgcg gtcgacttcg gcttcgcggt ggtcttgggc ttggaagcgg ccttcgacgt
     2581 ggcggtcgac ttcgactttg cggccgcctt tggcttcgac ctcgccgccg tggatttcgg
     2641 tgcggagccg ttcgacgacc tcgccccggt acgcgtgcgc cgcttgcttt gcccggactt
     2701 gctggtcgcc atcctcgtcc cttctgatcg atctggccct cctcgcttcg atctacccct
     2761 tgaaccgctt ctccaagccc ttccaggctc ttggtttacc gtgccgtcga actcgtggag
     2821 gggcagggat caagacgatg acgcgtccgt gggctggccc gctcgccctt acggcgaccg
     2881 ctgccctgct gctctcggtg gcggttctgg ccctgccggt tgacgcgcgg gcaaatcaga
     2941 atccgggcgc gcaggcgccg aacccgctcg tgggccagga gtggtggaac cagaagacgc
     3001 gctggaaccc gacgtggaac ggctaccggt cgctgctgcg gcgagacagg cgggccgacg
     3061 cggcgaaggt gttggcgttg gcacggacgc cgcaattcaa gtggttcggg atgtgggagc
     3121 agccaccggt cgcaaagctg cgcgggttgt tcgagaacgc cggcgacaag gttccgctgc
     3181 tcgcgatctt cggccacgac aacagcggct gtggcggcgg gttcaccggc ggcggggcgg
     3241 ccgaggacgc gcgttatcgc cgctggatcg gcaaggtcgt gcgcgggatc ggccccaatg
     3301 aggtcgtgat cgcgttcgag cccgattcgc tcggcacggt cgagtgcctc gtcccgtcgc
     3361 gtcgcgacga tcgcctgcgg acgctggcct acggggtgcg gcggctgtcg aggctgccca
     3421 acgcgacggt ctacgtggag gcgggcgcgt cggactggat gtcgccgcgg ctggccgcga
     3481 gccggctgcg cgcggtcggg gtcgggcggg tgaggggctt catgctcaac gcgacgcaca
     3541 tgacgaccac cgcggcgaac atccgccacg gtctcgcgat ctcacggctg gtcggcggca
     3601 agcacttcat cgtcaacacg tcgcacaacg gcaacggccc cctgtatcgc aacggccaca
     3661 cgatctggtg caacccgccc aacgccgcag cgggcgcccg ccccacgaca cggacggcgc
     3721 atccgaaggt cgacgcctac atgtgggtcg agcggccggg ctactcgaac gggtcctgca
     3781 acggcggtcc gctccctgtc ggcgcgtggt gggagcgcaa ggcgatacag ctcgtcgagc
     3841 gcgcgaagtg gtggcgccgg cgctgagtcg atccacatga aggcctgcgg cgctccaggg
     3901 cgaaactagc ggcatggata gaaagctcga cgtgggtggc accctaagcc aggccttctc
     3961 gatctacggc tcgcaggccg gggtgctgat acccctggcg ctcgcgctgt tcctggtcgt
     4021 cgccgtcgtc aacggccttg cggccgagtc ggtcgtgctg ctgccggtcg gcctcgcggt
     4081 cagcatcatc gcgggcaccc tctacaccgg catggtggtg gcgctcgtca gcgacgtcca
     4141 ggacggtagg cgcgactact cggtcggcga gctcgcgcgg tcggcgtcgc acgtgatcct
     4201 gccgctgatc ggcgccggcc tgctcgccgg catcggcatc gggatcggct tcctgctgct
     4261 cgtcatcccc ggcctgttcc tgatgacgat ctgggcggtg atcgcgccgg tgatcgtgct
     4321 cgagcgcgtc ggcgtcatga agtcgttcgg ccgctcgcgc gagctcgtca agggcgacgg
     4381 ctggcgcgtc ttcggcgtga tcgtgatcgt cttcatcatc gtcatcgtcg tccgcgcgat
     4441 cctcggagcg atcgccgtcg gcatcgacga cagcaccgtg gcgcagatca tcttcgactg
     4501 gatcggctcg tcgctcacgg cgccgatctc cgcactcgtc gcggcggtca tctacttccg
     4561 cctcaagggg gccaaggacg gcggcgcccc ggtgcccgcc gagcctgggc cggcgaccgt
     4621 ctgacggcat gaggcagcgg gtcagcctga tcacgctcgg ggtccgcgac ctcgagcgtg
     4681 ctcgcgcctt ctacgaggcg ctcggctggc agaccggcgc ggagcccggc gacgacatcg
     4741 tgttcttcca ggccggcgac atgatcgtcg cgctctggga tcgcgccagg ctcgctgagg
     4801 acagctgcgt cgaggacgcc ggcggctggg gcgggacgac gctcgcactg acgctcggct
     4861 cgccggccga ggtcgacgcc gcgatcgagg aggcgcgcgc cgccggcgcc acgatcggcc
     4921 gcgagcccgc cgataccttc tggggcggct acagcggcct gatgatcgac cccgacgggc
     4981 atccctggga gatcgcccac aacccccatt ggaccatcac cgaggacggc ggcgtccggc
     5041 tcgcctgatg caacggctct gcatcaacgt cgtcgagacc ctggaagccc cgagacagcc
     5101 ggcctagaat ctggggaatg accgccaagg agaagttgcg ggcgcaggtc gaggagctgt
     5161 cagaggctga ggcagccgcc gcccggatcg tcgtcgagcg gacgccgatc gatctcgaca
     5221 cggagccgga gatggtggga cttccggagt tgtggcggac cttcgaggac gggacacccg
     5281 tacccaactg ggtagccggt ctcgacgaag tccgtgccgg acgctgatgc tcgtcatcga
     5341 cgcgagtgcg gccacggcgg cgtgcgcgcg ggcagaaggc ttcgaggctc ttggtgacga
     5401 tctcgtcgca ccaccgctga tgtggtccga ggtgcgcgcg aatctgcacc tccaggcgtt
     5461 caagcggtcg atcagctacg accgggctga cgtcttgcat gaacgcctcg gcgcggcgtc
     5521 gatccgatgc gagaacccct cggacctcgg gcgaaacgcc tgggcgctcg ccaccgagat
     5581 gggatgggga cggacctacg atgccgagta catcgcgctc gcccagattc tcggctgccg
     5641 cgtcgtgacg gtcgatggtc gattgcgccg gggcgcgcat cggctcgggc tcgtcgtgac
     5701 gccggacgaa ctctgatcag tacggtcgtc gccgctctac cgggagcgtc gagcagcgaa
     5761 cgaccgctcg gccggaaccc gagtgcagcc attggcgggg tgcggctagt tggtcacgtg
     5821 gtcgtatctg cgccgtctgc gccaaccgag tgcgtgagat cagtagaggg ctcctcgacg
     5881 acgtactcga tgctgtcggc gtcgtcgctt acctcgtccg atatcgcctc ggccgactcg
     5941 attgacggca cctcgctcgt ctcaccccag cctgttagct ggaggaccgc actgtgaatg
     6001 gcttgagctc gcaagctcag aaaggtgtcg aagtcgttcg ctttcgcggc tgcgaacgcc
     6061 tccatcgaga tcaggttgga ggcgagccat gcctcaagtg catcgcccgc ggcctcctca
     6121 acacgcgtca agtactcgga gggaggggaa tccagaatct ccttgttgct gtttgaagtc
     6181 agcatcacga tgttcgcaag cgcattgatt cgaagacgag ggaccttctg aagtttgaga
     6241 taggcctgag ggaagaagtg gtgaaactcc ttgctgttca tccatgcgag cgccttggtc
     6301 gtgtcaatct gctgaccggt aagcaggtca acgggctggt ggtacgacag cacgatggcc
     6361 agcaacttcg cgtgcgcgtt gtttgatcgg aattgtcggg ttgtccaaat cgagacgctt
     6421 ggctcggtcg ccccaaccga tatctcagcg ctgtcgccct tggcgaacgc ttggactgcc
     6481 tcgagatcgg aacgcatcat ccccgtattc cagccactga agtagccagc caatgccgtg
     6541 cgccagaacc aagcacgtat ggcagcgtgc tgatcggcgc taggtgtggg cagtaagcgg
     6601 aagacctcag ccagcaccgt cagctggttg gagtacggga cgacatctcc gcccggtaca
     6661 cgcatgtcgg tcgcaaggaa atcgaccatc cgcttgtacg cgttgctgac ctcctcgacc
     6721 gcagctttga gctcatctgc gtcgtagttt cgaagcgcgt cgatactgtc ggtcgagaag
     6781 ccgcccccta tcgctgctgc gagattgcga aggacggtct tacgatcgat cgttccgaag
     6841 cccttcgcct ggaggcgatc gagtattccc tcgatcgaat caatgagatc gaagtccgga
     6901 ctccatgttg ctgctcgcat gaggtcgaca attgttagcg gcgtgcccat gctgtttatg
     6961 cgctcgaaga taggagcgac gtcctcgaga gacatatcgc ccaatgtgac cgccgcgatc
     7021 ttgtagtcct tgaagcgatt gaagaggacg ttggcgtgtg ccttcaagtc ctccttgtcg
     7081 tccgccatca gggaatccag gctggctaca tgctggaagt acgtcgcggg atccgcaagc
     7141 ttgttgatgc ggatctggtg gaggggcgga tcgtcagccg tatcgagatg gaggaacttc
     7201 ttctcgcgga ggtcgtaggc gatattccac ctgctacgcg cctcgccaac agtccaatag
     7261 agagcaccgc agatggtgga aagtctctgc tgaccatcaa gcaggtagtt gaccggatag
     7321 tccggcttcg gcagatcgat ctctaggtcc gcaatgcggt tctcgctacg gagctcctgc
     7381 ctgctctgcc agagcaacac gctcccgatg gggtacccgc gagccacgga gtcgaggagc
     7441 gtgagaattt ggggccgctc ccacacaaaa ctccgctgga acttgggaag caggatgtca
     7501 ccgctgagga ttcgtcgggc gagctcatcg atgcggtcaa cgctcggctg tggatccttc
     7561 gggactgccg ccgtgggtcc agatatctct acctctgcgt ccgtcacttc gtcctccgtc
     7621 cctcgacgat tcggagttgt ggatcgtagc cgcgacagaa gacgcgtatt agtggtaccg
     7681 gaactggtac cgccggctga tcggccggcc cggcgggcaa cttgctagcg acgagagccc
     7741 gctcccgtag cgggctccat ccaatcgggg cgactggatt tgaaccagcg accgctcggc
     7801 ccccagccga gtgcgctacc agactgcgcc acgccccgag ggggcggatg ttagacggcg
     7861 cgggacggat tcgccagcgg cgcgggggat cctcgggtag cttctgcgca tgccagatca
     7921 gccgaaggtc gtcttgcacg ccgtcccgtt ctcgcatccg tgcctcgcgg ttcaggcggt
     7981 gctggaccgc cacgggctcg agtacgagaa ggtcgatctg gtctcggggc cgcacgccga
     8041 cgagatcgag cggatctacg gcgagggcaa gcggacggtg ccggggctgc tggtcgacga
     8101 cgagccggtc cacggaacga cggcgatctt cgagaagctc gacgagctgg tcgacgacgc
     8161 ggacctctac ccggccgcgg tcgcggcggc gattcgcgag gccgagcggg gcatcgccga
     8221 ggacctccag acgacggctc gcaacctggt cttcggggcc ctgcacttcc gtcccgaggc
     8281 gctcgggacg ttcgccggca ccgggccgct cgaccccgcc ggcaccgact tcgcgatcag
     8341 gatggtgcgc ggggcatggc gctacatcgg catctcggcc gagctgctcg ccaccacgct
     8401 ctcggagctg cccgagcagc tcgacatcgt cgacgagctg atcgagggcg gcgtcgcggg
     8461 cggcgacgat ccgaccgccg tcgacttcca gctcggctcg tcgctccacc tgctgctgca
     8521 gatcggcgac gtgcggccgt tgatcgaggg ccggccggct ggggcgctgg tcgaccgctg
     8581 gttcgagccg ggccccgcgg acatcccggc cggggccttt ccggccggct gggtgcccga
     8641 gcggtctcag agcgccgcct gagccaacat cgccgtcccg gcgacgacga gcatcgcgcc
     8701 gatcgcgcgc agcagggcgc gctccgacag tcgcccggtc agccgcgcgc ccagcaacgc
     8761 tccgggtaca gacgccgccg cgccgagcgc gagcacctcc cagtcgacgc ccgccggcgt
     8821 gtggccgatg acgccggcga cccccacgca cacgcccacc gacaggttcg tcccgaccgc
     8881 tcgatgcgcg ctgccgccgt gccagcgcag cagcgccggc atccgcagcg ccccgaggat
     8941 cagaccgatc aggcccccga gcaggccgat gccgagcccc gccagcgcaa gcgctacgag
     9001 atccggaccg tcctggtcct cggtcgccga gcgtcgctcc cgcctgcgca gcaggtcgaa
     9061 gccgaagtag agcagcgtcg tcccgatcac cgccagcagc accgcgtcgg gcagcaggcc
     9121 cgcggcgtag cccccgatca gcgcgccggc gaccgagggc ggcgtcatcc aggcgaagac
     9181 gcgccagtcg atccgtccgc cgcgcacgtg gacgatcgcc gccgcgagcg ccgccgcgcc
     9241 tgagattccg atgttcgcgc cggctcccgc ggccgggctc gacgcgacag ccaccacggc
     9301 cggcaggcgg atgttgccga gcaccagccc gaccgtcccg ccggcgaccg ccaccgcgaa
     9361 gcaccagacc agcagggcgg tcagcgctag cggatcgccg tccacgtccg cctagtgcgt
     9421 gtgaccgtgc ggctcgacgg cggctagctc gtcggcggac atgcggcggg ccagcatggc
     9481 ggcgagcgca gtggtgatcg gcaccgccgc gatcaggccg atcgagccga ccaacgtcgc
     9541 gacgatctcc ttggcgatga tctcgaggtt gaccgcgtcc acgacgtcga tctggccgga
     9601 gctgaagatc agcagcagcg gcagcgacgc gccgacgtag gcgagcacga gcgtgttgac
     9661 ggtcgcgctg acgtggtcgc gcccgacgtc catcgcgagc ttgaagagct ggcggaagct
     9721 ctgcccgggg ttcgccgcgc gcaacgcgag caccgtcgac gactggctga ccgtgacgtc
     9781 gtcgagcacg ccgagcgccg cgacgatcat cccggcgagc agcaggccct cgagcgagac
     9841 gcccgagtcg ttgatctgca ggaacgtcgc ctcctcgctc gccaggccgg tcaggtgggt
     9901 gaggtcggtg aagaggaccg ccagcccgac cgtgacgacc agcgaggccg cggtcccgac
     9961 gatcgccgcc aggctcttcg gattcgcccc gtgggcgagc aggatcgtcg tcagcatcac
    10021 cgccaccgag ccgacgatcg caacggcgag cgtgctcttg ccgtcgagga tcgccggcac
    10081 catgaagccg agcacgaccg cgaggctcag gcccagtccg atcagcgaca gcgcgccgcg
    10141 caggcggccg aacaggacga ccacgccggc gaagatcagc gccagcagca gcagcggctc
    10201 ctggcgatcg aagtccgaga tcgagtactc gcgtcccgga acgggatccg cgcccggcgg
    10261 cacgacgttc ctgacgacgc gcagctcgtc accgaccgag acctcgggat cgatcgtgcc
    10321 gtcgtcgagc acgagctcct tggtcgcgcc gctctgcgcg ccgctctcga gccggatcct
    10381 tacctcgcgg cagacgtcgg tctccagtcc cgcgcaggcg aactcgctga cggacaggac
    10441 cgtcgcggtg tcggacttgg tgacgatcgc ctcgccgacc cgcgtgtcga cgtcgccggg
    10501 ccagaggatc gccatccccg cgagcgtcgc gacggcgagt gcggcgacga tcgtcgcgaa
    10561 gacacggatg acgttgctct ggcggatgcg cggaatggaa gcagcggtcc cggaccaata
    10621 ggggcaccgg atcgcgctcg atgcggccga aggcgcccgc gccgacgcga aagcgccggc
    10681 tgtgtcaccc ttaccggctg atggcacgcg gcaacacgaa ggaccggaag cgcggcaagc
    10741 cctccttgaa gggggaggcg cgccgctact ccaagctgcc gcgcgaggag atcgagaaga
    10801 tcgactacaa gaacatcgcg ctgctgcagc ggttcgtcag cgagcgcggc aagatccgct
    10861 cgcgccgcgt gaccgggctc agccgtcgcg atcagtcgaa gatggcgcgc gccgtcaagc
    10921 gcgcccgcga gctcggtctg ctgccctacg tcgacgccag caaggccgtc gagcgggtcg
    10981 gcggcggtcg cggtcgccgc gagcgcgagt agctctctcg ctcagcctcc gcgctcgagc
    11041 tcgatcgggc cctgctcggc gtgggcgtgg tcgacctgga tcgtcgtgtg gtcgatcccg
    11101 aagcggtcgt gcaacatcgc ctcgaagtcg cgtcggcgct ggtggcagtc gcagtcgggc
    11161 tcgagcagga cgtgggcggt cagggccggg aacccggacg tgacctccca gacgtggagg
    11221 tcgtgcacgc cgacgacatc gggctcgtcg gccagcgcct gcgcgatctg gttcgggtcg
    11281 atgccgcgcg gcgagccctc gagcaggatc cggcccgagt cgcgtagcag cccccagccg
    11341 gcgcgcgtca tcagcgccgc gacgatcagc gcggcgatgc cgtccgcctg cccgaacccg
    11401 gtcgtcagga tcacggcgcc cgcgacggct gtggcgatga aggcgaacag gtcggtgagg
    11461 atgtgctgga aggcgccctc gacgttgagg ctgtcgcggt tcgcgccggc cagcacccag
    11521 gtcgccaaca cgttgacggc gacgccgacg agcgcgacga cgagcacgag cccgccctcg
    11581 accgacggcg ggtcggcgat ccggcggacc gcctcgaagc cgatcaccgc ggcgagcgcc
    11641 agcagcgtcg ccccgttggc ctgcgccgac aggatctcgg cgcgccgcag gccgtaggtg
    11701 tagccgccac gcggcggacg agcggcgagg cgggcggcga acagcgcgag cgcgatcgcg
    11761 gcagcgtcgg tcagcatgtg agccgcgtcg gagagcagcg cgagcgagcc ggcgacgatc
    11821 ccggcgacga cctcgcccgc catcagggcg aggatcagcc cgagcgcgat ccagagccgg
    11881 cgaccgtcgc cctcgcgggg gatgccgtgc gcgtgggctg agtggtcgtg ggccatgcct
    11941 ccggccaggc taggcgaccg caggcgccgg ggagcgattc gcggtggcgg acccgtcggc
    12001 agtcaaacga gaatgattct cagtatggtc tcgcgtgatg tccaggcgcc ggtcgaccat
    12061 cctcgtctcg atcgtcctcg ccgcggctct ggcgatcggc tgcggcgacg acggcggctc
    12121 cgcggcgcgc ggcgagaagg ccctgaccgc tgtcgctacc acgacgcagg tggcggactt
    12181 cgcccgggag gtcggcggcg accgcgtcga cgtccggcag atcctgcagc cgaactccga
    12241 cccgcacggc tacgagccgc gacccagcga cgtggcgtcg gtcaccgagg ccgacgtggt
    12301 gatgcggtcc ggcggcgacc tcgacgcctg gctcgacggg ctgctcgaca acgcgggcgc
    12361 cgacgtccca caggtcgagt tgatccaagc ggtcgagcgg cgcgacggcg gacacgcggg
    12421 cgagggcagc cacgaccacg tcggagagga ggagcacgac catggcgaca gcgccgaccc
    12481 gcactggtgg caggacccgc gcaatgcgat cgctgcggtg gcggctatcc gcgacgcgct
    12541 cgcagccgcc gatcccgacg ggcgagcgga gtacgagagg aacgcggccg cctacatccg
    12601 gcagctgcgg cgcctcgatc gggcggtggc ggcctgcatc gacgaggtcc ccgcgccgca
    12661 acgcaagctg gtcaccaccc acgacgcgct cggctactac gccgaccgct acggcatcga
    12721 ggtcgtcggc gcgctgatcc cgtcgcagtc gaccgccgcc cagccgtcgg ccggcgacat
    12781 cgaggagctc gtcgaccaga tcgagcgcga gcaggtcgag gcgatcttcc ccgagagctc
    12841 gatcgacccg aggctggagc gggcggtctc gcgcgagacg ggggcgaccg tcggcaaggc
    12901 gctctgggcc gacacgcttg ggggggaggg gtcgggcggc gccacctacg tcgagtcgat
    12961 ccgctcgaac accgccgcgc tcgtcgaggg cctcaccggc ggcgctcagc ggtgccggcc
    13021 ctgagcccgg cggtcgctct caggcggagg cccgacacgc cgggcactcg ccgtgcagcg
    13081 tgacgtcatg agcggcgatc gcgaagtcgg tcttggccga cagcgcgtgg atcgcctcct
    13141 cgagcagctc gtcctcgaac ggctcgaccc tgccgcagct ctcgcagacg agatggtggt
    13201 gatggtcgcc gcccggaagc gcagcctcgt agcgggcgac gccctcgccg acgtcgaagc
    13261 gctgcaccag gccgaggcgg tcgagcaggt cgagcgcgcg gtagatgctc gccaggccga
    13321 cgccgttgcc gcgctcgcgc agcaggtcgg cgatctcgcg cgcggtcatg ctgcagccga
    13381 gctcggcgat cgcctcgatc accgcttcgc gagccgcgct cgagcggtag ccggcatccg
    13441 cgagcgtcct ggcggtgtgc tcggtccagc cgctggccgt cgtcgaagcc atctcggaga
    13501 gggtacttgc cgctagggtc gcgccgtgga gatccatcgg accccggacg agcgtttctc
    13561 agacctgccg ggatacccgt tcgagccgca ctacgccgag gtcggcggct tgcggatgca
    13621 ctacgtcgac gagggggagg gcagcccggt cgtctgcttc cacggcgagc cgtcgtggtc
    13681 gtacctctac cgcaagatgc tgccgccgct cgtggctgcc ggacaccggg tgatctgccc
    13741 cgacctggcc ggcttcgggc gctccgataa gccgatcgac cgcggctggt actcctacga
    13801 ccgccacgtc gagcaggtcg cgggcgtgct cgacggactc gacctcagcg gtgcgacggt
    13861 ggtcgtccag gactggggcg ggccgatcgg cctgcgctgg gcggtcgaga acgccacgcg
    13921 cgtggaccgg ctggtgatct tgaacaccgg cctcttcacc ggcagggtca gcaagggctt
    13981 cctgcagtgg cggtcgttcg cggagcagaa ccccgacctg ccggtcggga tggtcgttca
    14041 gggagcgact acgaccgatc tgccgccaga ggtggtgacc gcctacgagg cgccgttccc
    14101 ggacgccgag tcgaaggccg gcgcggcgac gttcccgctg ctggtcccga tcgacgagag
    14161 cgcgcccggc gcctccgaga tggcggccgt gatggacgcc ctgtcgcgct gggacaagcc
    14221 ggcgctggtg gccttctccg actcggatcc ggtgttcccg tcgccgaagg ccggtcaggt
    14281 cttcgtcgac ctgatccccg gcgccggcga gcagctggtg atcgagggtg gcggccactt
    14341 cctccaggag gaccgcggcg aggtcatcgc cgcggccatc ctgaactggg agtagaaccg
    14401 gggctcccgc ccccggaccc ccgtggcgcc tggctggcgc tggggcaccg gttccgtccc
    14461 cggacccccg tggcgcctgg ccggtgcgcg tctgttaggc gacggcgctg aagcgctcgg
    14521 cgaccttgga ccagtcgacg acgttccacc acgcgtcgat gtagtccggg cgcttgttct
    14581 ggtacttgag gtagtaggcg tgctcccaga cgtcgacgcc gagcagcggc gtcttgccgt
    14641 cggtcagcgg gttgtcctgg ttcggcgtgc cgacgaccgc gagtccggat ccatcgtgga
    14701 ccagccaggc ccagccggag ccgaactggc cgacgccggt cgccttgacc ttgtcctgga
    14761 agtcgctgaa cgagccgaag gcggagtcga tcgccgcggc gagatcgccg tcgggctggc
    14821 cgccgccgtt cggcgacatc gactcccaga agagcgtgtg gttgaggtgg ccgccgccgt
    14881 tgttgcgcac cggcccgcgg atgtcgtcgg gcagctgcga aaggttggcg atcacgtcct
    14941 cgatcggctt gccggcccag tcggtgccct ccagcgccgc gttggccttg tccacgtagg
    15001 cctggtgatg cttgtcgtgg tggacgcgca tcgtcgcctc gtcgatgtgc ggctcgagcg
    15061 ccgcgtagtc gtacggaaga tccggtacct cgtaagccat aggcattcct ttcgtggtct
    15121 cgcaggcgag tgtattgctc gcccgccgtc aggctcggtc gggcgcgagg tcgagctcgg
    15181 cgtcggcgta ggtaacgccg atcggctcga cgatcacctg gcgcgggccc tcctcgaccc
    15241 ggccggcgac gagcgcctcg tgccccaggc cgcgcgcgat ctcgaccacc cgctcagcgc
    15301 tgccggccgc gctgtagacc gcaaacccgc tgcccatgtt gagcgtgctg tacgcggcgt
    15361 gggcgtccat ctccgcctgc tcggcgagga aggcgagcac ctccggcacc tccggaagcg
    15421 cggtgatccg gtaggtgagg tcgcggctcg ggcgcatcag cttcagcagg ccgtggccgg
    15481 tgacgtgact gaggtagctc accggcacgt ccgcctcggc tagcgccccg acgagtcgcg
    15541 cgtagaggat cgacggatcg agcagcgcct cgccgtagga ccggccgctg gggagcgggg
    15601 tggcgtagcc gttcgcgagg cggtcggcga tcatccgcgc gagcgaggcg ccgttggcgt
    15661 gcaggccgtt gctcgcgacg atcatgatct cgtcgccggg ggagaggtcc tgcccgagga
    15721 tcggctggcg gccgtccggg agcgcgccga cggcgcttcc cgccagctcg atgtcctcgc
    15781 cgttgacgag ccccggcagc gacggcgact cgccgccgcc ccaggtcgcg cccgcgtcgg
    15841 cgcacgcccg gctccagccc ttgaccagcg cggagtgcca ctccgtgttg cggtaccagt
    15901 ccgacgatcc agtggcgaag tacgcgttca cgacgagcgg cagcgcgccg acgcagcaga
    15961 ggtcgttgac gatcgcggcg accgtgtcgt aggcgacggc gtcgaagcgg ttcaggccga
    16021 gctgctcggc caccagtcgc gacgccaccg ccttcgtccc caggccctcc atcacgaacg
    16081 ccagcgtccg gtcgccgagc tcgaacacga acgcgggctc gccccgcgac acgtcgagcg
    16141 cccgcccgcc atgcgctgcc agcgccgccg acgttccgag cgcctcggtc agtgcaagcc
    16201 gcttaccggc gtcgagcgtc gcgtagtcga ctcccgcgct ggcgtaggcc gactcgccct
    16261 gactcggatc gctcatcgga tgcgctggag gccggtctgc aagtgcggat gagaggactc
    16321 gaacctccac gtcccgaagg acacaggcac ctgaagcctg cgcgtctacc aattccgcca
    16381 catccgcgtg gggcgtggaa ccttagctgc cggcggggtc agtcgcgggt cttgcggcgg
    16441 cgcagccggg tgccgaaggc ggcgtcccac cagggggcgg cgacgccgaa gccgcgggtg
    16501 tggtcctgga agtggtggcg catgtgaagc tcgtagaaga gcttgccgag acgggtgcgc
    16561 ggcttgtggt ggtggaggtg gtagtgggtc atgtcgtagg cgaggtagcc gacgaggaag
    16621 ccgcaggcga agaggtcagc gctctggccg ccgatcgccc agtggaacag cgcccagaac
    16681 gccagcgcga gcgggacgct gaccgacggc ggcatcacca gccgcatcgg gtcgttgggg
    16741 tggtcgtgat ggacgccgtg gatgatccag tgcagccgcg cgccgatccc gtcctcgggc
    16801 tcgaggtgga agacgaggcg gtgcagccag tactcggtca gcgtccagaa gacccagccg
    16861 ccggcgatcc aggcagtcac ctcgaacggc ggggtgccct cctgcccgag cgcgaacagg
    16921 acggcgatcg cggggatgaa gatcaccaac gggactacgg gatgaacgcg cgagagccag
    16981 tcgagcaggt tcgactcgaa catccgcgga gaggccttca ggacatcgga gcgccgctcg
    17041 tccatcggga tcaatgtaac ggacgccgcg ggcgccgacc atccgccgct tagggggtcc
    17101 taactggcgt tctcaccgcg cgggcgtacc gggtgcgcgg cacgcaggaa ctcaaggccg
    17161 aagccggctg tcagcgcgta cgccgaagcg atcgcgaggc ccggcagccc gcgccgcgcc
    17221 tgctcgaagg tcatcgccag caaggtggcc gtgaccgccg ccatgaacgc ccaccggatg
    17281 taggcgtgga tcgtctcgat cgtcgtgctg ttcatgcgcc catcgtggga ccgctgccga
    17341 gccgcgctgt gatcgacggg tgaagccggt gtcaaaggga tgtgaacccg ggctcgcgga
    17401 tcgccgcaaa gacgccgacc tgggccgcct cgccgagcgc ctcccagctc gtctcggcgg
    17461 tggttcccgc ccagtcgacg cagtgcgcac gcgcctgctc gagcgagtcg aagccggcga
    17521 tcgcgattgc gttgtcggtg ccggcctcgt gctcggcgtc gacggtgacg acgagcaggc
    17581 gaggtgtcgc gtctccgacg gcgacgcggc cgttctcgcc ctgccagacg agcaccgcgg
    17641 tccgctcgcg gtcgtgctcc tccgagcggt agatcgcaag cgtccgggcc cgggtctcgt
    17701 tccacgacca gggccttctc atccgtgtcc tggttgaggg cacccgcacg tcgacgactc
    17761 taccgccgca agggttaccc acatagaaat ttataccctg gtaacatggt ctgcctgatg
    17821 gccgtctcga gcaccagtcg aacccgcctg ccgcgcgcgg agcggatgga gcagaccgtc
    17881 gctgcggccc acgcgctgtt cgccgagcgc ggctacgccg ccgtgacgat ggacggcgtc
    17941 gcggcggccg tcggcgtcac caagccgctg ctctacaact acttcggcaa caaggagcag
    18001 ctctacctcg cgtgcctcga gcgggcgggg gacgggctcg tcgcgaccgt cgtcgaggcg
    18061 gtcggcgaga cgagcaaccc gaccgaggcg ctcagcctcg gcctgcacgc cttcttcggc
    18121 ttcgtcgacc aggaccgcgc cgcctgggcg gtgctcttcg acgagacgct gcccgctggc
    18181 ggtgagatcg ccgagcaggt cgccggctac cgcacccgcc tgcgcgacct gatcgccgcc
    18241 tcgatgctcg agcagctccc gccgcgcagc cgcgagcgcg cgcgcaccga ggtcgaggcg
    18301 ctctcgacgg cgatcatggg cgccgccgag gcgctcgtgc gctggtggct gcgaaccggc
    18361 gcgatgcccg ctgccgacgt cgccgacctc ttgatctcga ccgtcgagcc cgggctgcgt
    18421 cagcgctcgg cgcccgccaa gtcttccaag ccgaactccc caggaggcga tccccccaga
    18481 tgaacagcaa gacccgcccg gttgccgtcg tgggcggcaa ccggatccca ttcgcgcgct
    18541 cgaactccgc ctactcgaag gcgtcgaacc aggacatgct gaccgccgcg ctcgacggcc
    18601 tgatcgaccg cttcgacctc gacggcgtcg agctgggcga ggtcgccggc ggcgcggtgc
    18661 tcaagcactc gcgcgaccgc gacctgacgc gcgagagcgt gctcggcact cgcctggcgc
    18721 ccgagacgcc cgcctacgac ctccagcagg cgtgcggcac cgggctcgag gcggcgatcc
    18781 tcgtcgccaa caagatcgcg ctcggccaga tcgactccgg catcgcctgt ggcgcggaca
    18841 ccacctcgga cgcgccgatc gccctcaacg aggacctgcg cgaggtcctg ctcgaggcca
    18901 accggcagcg ctcgacgacc ggcaagctga agcagctcgc aaaggtccgc ccgggccacg
    18961 tcgtgccggc gatcccgcgc aacgaggagc cgcgcaccgg catgtcgatg ggcgagcact
    19021 gcgcgatcat ggcgaccgag tggggggtcg gccgcgccga gcaggatgag ctgaccgtgc
    19081 gcagccacga gaacctggcc gccgcctacg agcgcggctt catggacgac ctgatgacgc
    19141 cgtacctcgg cctcgagcgc gaccagaacc tgcggccggg atcgtcggtc gagaagctcg
    19201 gcaagctgaa gcctgtcttc ggcggcgagg acgggacgat gacggccgcc aactcgacgc
    19261 cgctcagcga cggcgcgtcg gcggtcctgc tggccagcga ggactgggcg cgtgagcgcg
    19321 ggctgccgat cctcgcgcgc ttcgccgatg gccagacggc ggcggtcgac cacgtccaca
    19381 agcgcgaggg gctgctgatg gcgcccgcgt acgcgatgcc gcggatgctc gaccgcgccg
    19441 gcctgacgct gcaggacttc gacttctacg agatccacga ggccttcgcc gcccaggtgc
    19501 tctcgacact gcgcgcctgg gaggacccgg tcttctgcaa ggagcggctc ggccgcgacg
    19561 cgccgctcgg agcgatcgac cgcgacaagc tcaacgtcaa cggcggctcg ctcgccgccg
    19621 gccatccgtt cgccgcgacc ggcgggcgga tcgtcgccag cctcgccaag gcgctgcacg
    19681 agcgcggctc cgggcgcggc ctgatctcaa tttgcgcggc cggcgggcag ggcgtcgtcg
    19741 cgatcctcga ggccccggga ggcaacagca agtgagcgac cgttactcgc agatcgtcaa
    19801 ctcgttcccc ggcaagctgg tcgccgggcg cgtcgggctg ccgcagccgg tcgagctaga
    19861 ccgacaccgc cccggcgcgc cggtcgtctc cggtcgcgtg ctgctcggcg cggcgcccgg
    19921 agggcggctc ggtacagcgg tcgcgcgagt cctggcggcg gccggcgccg agacatcgac
    19981 cacgcccgat ccgacgctgc gcgaggcggc ggccgcggca agcctcgacg cggctctgtt
    20041 cgaccccgag agcggcggcg accagcgctt caaggcgctc gtcttcgacg cgaccggcat
    20101 ctccgactcg accgagctcg tcgagctgca gcgcttcttc cacccgaccg tgcgcaagct
    20161 cgagcgctcg ggtcgcgtcg tcgtgctcgg cacgccggcc ttcggctgcg acacaccgcg
    20221 cgagtcgacg gcccagcggg cgctcgaggg cttcacgcgc tcgctcggca aggaggtagg
    20281 ccgcggtacg accgtgcagc tcgtctacgt ctcgccgggc gccgaggacg agctcgactc
    20341 gacgttgcgc ttcctgctgt cgccgaagtc ggcctacgtc tcgggacagg tcgtgcgcgt
    20401 cggcccgggc gtcgccaagg cgccgaagct cgactgggac aagccgctcg ccggcaagcg
    20461 ggcgctcgtg accggcgcct cgcgcggcat cggcgccgcg atcgccgaga cgctgcgccg
    20521 cgacggcgcc gaccttgtgc tgctcgacgt cccggcgctg tcggccgacc tcaagcaggt
    20581 cgccaagcgg ctcgacgccg acacgatcga gctcgacatc accgaggagg gcgcgcccgc
    20641 ggcgatcgcc gagaagctct ccgacggcgt cgacgtcgtc gtgcacaacg ccggcgtgac
    20701 gcgcgaccgg acgatcgcca agatgcccga ggagcgctgg accgggctga tggagatcaa
    20761 cctctccagc gaggagcgga tcgacgacgc gctgctcgag gccaatctcc tgaggcccaa
    20821 cggccggatc gtctgcgtct cgtcgatgag cgggatcgcc ggcaactccg gccagaccaa
    20881 ctacgcgacg tcgaaggcgg gcgtgatcgg gatggtcgag gcgctcgcgc cgacgctcgc
    20941 ggagaagggg ctgacgatca acgcggtcgc gcccggcttc atcgagacgc agatgaccgg
    21001 tgcgatgccg atcgcgatcc gcgaggccgg ccggcggatg aacagcatgt cgcagggcgg
    21061 cctgccggtc gacgtcgccg agacgatcgc ctggttcgcg agccccgcgt cgaccggcgt
    21121 caacggcaac gtcgtgcgcg tctgcgggca gagcctgctg ggggcctgat gaggcaacgc
    21181 acgctcaccg gtccgccgag catcctgccg ctctacgcgc gcgccgcggc gccgctgatc
    21241 cccggcgcgt cgctgctgcc gttcgtgccg ggcggcggca aggagatccc cgacgtcgag
    21301 ctgacgctgc ccgacgtcgt cgccgactcc gaggcggtcg ccgcctacgc caaggtctgc
    21361 ggcttcacgc tgcgcgacag cctgccgccg acctacccgc acgtgctggc gttcccgctg
    21421 cacatggcgt tgatggccga cggcagcttc ccgttcggcg ccgtcggcct cgtccacgtc
    21481 gagaaccgga tcgttcagca ccgcccgatc ggcctcgcgg agcagctgac attgcgcgtg
    21541 cgcgggacga agctcgagcc gcatccgaag gggcggacct tctcgatcgt ctccgaggcg
    21601 cgcgtcggcg cggagctggt gtgggaggac gtcagcacga tgctgcgccg cggcggcggg
    21661 cgctccgagc ccaacggcgg caaggacgcc cctgggccgg ccgacgcgga caacgccgag
    21721 caggccgcca gcgccgagtg gaagctcggc ggcgacctcg gccgtcgcta cgccgcggtg
    21781 tccggggacc gcaacccgat ccacatgcat tcgctgacgg ccaagccgct cggcttcccg
    21841 cgcgctatcg cccacgggat gtggaccaag gcgcgcgcgc tcgcggcgct cgagggccgg
    21901 ctgcccgacg ccttcgccgt cgacgtccgc ttccgccggc cgatcctgct gccggcgacc
    21961 gtcgcgttcg ccagcagcga cagcggcgac gagatcgact tcgcagttcg cgacgcaaag
    22021 cgggggaccc cgcacctcga cggccgcgta gcgccgctcg aggcgaagaa gaagaccagc
    22081 accggaagaa agagggcctc atgaacgcca ccaccgacaa gggcatggcg gagcgcggga
    22141 tgggcttctg gctgcgtgcg ctgaaccgcc tcgccggctc cgacctgctc gaccggatcc
    22201 gcatgcgcaa gcaggtcgag cgcgcgctgt tccagggaac caagcagggc ttccgctcgg
    22261 cgaccgccgc cagccgcagc ttcaaggctg ctcagaagct cggccgtccg gcgcgccagc
    22321 cgcacgccaa gccgcgcggg ctgttcgaca tcacgcccga cgacgagcag gcgatgctcc
    22381 aggaggcagt cggagcgttc gccgccgagc aggtccgccc gatcgcgctg gaggccgacg
    22441 ccgcctgcgc gacaccgccc gagctgctga cccagtcgac cgagctgggc gtctcgatgc
    22501 tcggcatccc cgaggagctc ggcggcgtga tgcacgagca gtcggcggtg accagcgtgc
    22561 tgatcggcga ggcgctcgcc cacggcgaca tgggcgtcgc ctacgcggcg ttggctcccg
    22621 gcgcggtcgc gaccgcgatc gcccactggg gcgacgccga ccaggaggcg acctacctgc
    22681 cggcgttcgc cagcgagaac gtcccggctg ctgcgctcac gctgctcgag ccgcgaccgc
    22741 tgttcgaccc gctgaagctg gagacgacgg cacgcaagga tggcgaggac tgggtgctcg
    22801 acggcgtcaa atcgctcgtc gcgcgcgccg ccgactgcga gctcttcgtc gtcggggcgc
    22861 aggccgaggg gatcggcccg gcgctgttcg tcatcgagtc ctcgaccggc gggctgtcgg
    22921 tcgagtccga gccggcgatg gggctgcgtc ccgccgccac cggacggctg ctgctcgagg
    22981 gcgtccggct gccggccggc gcgctgctcg gcgacggcga gcccggcgtc tacagcgagt
    23041 gcgtcgaccg cgcccggatc gcctggtgct cgctcgcggt cggcaccgcc caggccgtcc
    23101 tcgactacgt gatcccgtac gtcaatgagc ggaccgcctt cggcgagccg atctcgaacc
    23161 gccaggcggt cgccttcgcg gtgtccgaca tcggcatcga gagcgacggg atgcgcctcg
    23221 cgacctaccg cgccgcgagc cgcgccgacc acggctctga cttcgcccgc gagacggccg
    23281 tcgcgcgcaa gctctgtgcc gacaagggga tgaagatcgg ctccgacggc gtccagctgc
    23341 tcggcggcca cggctacatc aaggagcacc ccgtcgagcg ctggtaccgc gaccttcgag
    23401 ccgcgggcgt gatggagggc gccctcctgg tctaggcgcc gcctcgaccg gaaaggattc
    23461 acatgatcga tctagagata cccaagaagt tccgcccgct ggtcagccag gccaaccagg
    23521 tcgcgaccga ggtcttccgc ccgatctcgc gcaagtacga cgtcgccgag cacgagtacc
    23581 ccaaggagct cgacatgctc gcgtcgctga tcgacggcat gaacgagggc agcgacctcg
    23641 gcggggccgg ggcggccggc gtgcgccgcg aggagaacgg cggcgaggcc aagaaccgca
    23701 acggctcgaa cctctcaacg gtgctcggga tcatcgagct ctgctggggc gacgtcgggc
    23761 tgctgctgtc gatgccgcgc caggggctcg gcaactcggc gatcgcgtcg gtcgccaccg
    23821 aggagcagct cgagcgctac ggcggcaagt gggccgcgat ggcgatcacc gagcccgagg
    23881 ccggttccga ctccgcctcg atccgcacga ccgcggagct cgacggcgac gagtacgtgc
    23941 tcaacggcga gaagatctac gtcacgtccg gcgagcgcgc cgacctggtc gtcgtctggg
    24001 catcgctcga ccgctcgaag ggccgggcgg cgatcaagtc gttcatcgtc gagcgctcga
    24061 accccgggat gaagctcgac cggctcgagc acaagctcgg catccgcgcc tccgacaccg
    24121 ccgcgttcat cctcgacaac tgccgcgtgc cgaaggaggc gctgctcggc agtcccgagg
    24181 tcgacgtcaa ggccggcttc ggcggcgcga tgcagacgtt cgacaacacg cggccgctgg
    24241 tcgccgcgat ggccgtcggc gtggcgcggg cgtcgctcga cgagacgcgc acacgactcg
    24301 ccgaggcagg ggtggaggtc gactactcgc gccccacgct ggcccagtcc gccgcggcgg
    24361 cgcgcttcct cgagatggag gccgactacg aggcgtcgcg gctgctgacg ctgcaggcgg
    24421 cgtggatggc cgacaacggc aagccgaact cgctgcaggc gtcgatggcc aaggcgaagg
    24481 cggggcggac ctgcgtcgac gtgtcgctcg gctgcgtcga gctgtgcgga tcgctcggtt
    24541 acggcgagaa cgagctgctc gagaagtggg cgcgcgacgc gaagatcctc gacatcttcg
    24601 aggggacgca gcagattcag ctgctgatcg ttgcgcggca gctgctgggg aagtcgtcgg
    24661 ctgagctgaa gtagggctcg ggcgctggct gcccgcgggt gctgtctctt ggagtgcacc
    24721 gctcgcaagc tcgcgtcgtg agccgggaga acccgtgctc gcaagctgcg cgcccaaacg
    24781 caaaatcgtc gccacccgcg gaggccggcg tctacagctc actgcgcggg gccgaggcgc
    24841 tgctaagcgt cgcggccctc gaggccctcg aagaggaccg aggcgcggac gtcgaggccg
    24901 cgggcgagct tggagacggt gcggatgccg ggctcgcgct cggcgcgctc gatgcgggcg
    24961 acgtgggtca tgttgaggcc gcagaggtgc gcgagccgct cttggctgag cccgcgggcg
    25021 agcctggcct tgcggaggtt cgatccgaac tgcggaaggg gatcgtccac actcgcatac
    25081 ttcgcctcta gggccaggcg cgccagagcc gattggcaca agaagggccg gtcggcgttg
    25141 ttttggacaa catggcatta cgggggagaa ccggcgacca tcgccggcca tgtccagaga
    25201 cgttctcgtt ttctttgcgg agaacctgcg ccgccggcgc aaggagctcg gcctcagcca
    25261 ggagcagctc ggcgcgcgcg ccgacatcca gatggccgac atctcccgct acgagagcgg
    25321 cagccgcgac ccgcggataa cgacggtggc gcggctcgcc gacgcactcg aggtctcgat
    25381 cggcgacctg ctgcaggcct cggccgacgg ccgggtcagc tgagctagcg gccgcttcgc
    25441 aagtacgctc agctagccgc gccggcggta gtgcaagtac accgtcccgg cgaacgtgcg
    25501 ggtctcgacc aactcgaggt cgacgtgcac gtccggtggg aagtacggcg tcccgccgcc
    25561 gatcacgatt gggacgacga acagctggaa ctcgtcgatc agcccgcgcc gcatgcacgc
    25621 cgccgcaagg ccggcgcccc cgaccgcgat cctgcgatcc ccgagcgcag cgatctcctc
    25681 atcgagcgtt ccggtggcca ggcggttgct gccctcgacg ctcgtcaagg tggtcgagaa
    25741 gacgaccttc tccagcgggc gccagatctc ggcgaactcc gccatcgcga ggtcggagaa
    25801 ctcctcgacg tcccacacca gcatcgtctc gtagaggcgc cggccgagca ggtggacgtc
    25861 ggtcgccgcg gtccgttcgt tgtgaaaccg gtgcagctcc tcgctgggcg ccgtccagtc
    25921 gatgcttccg tcgggcccgg caacgtagcc gtcggccgac acgctcatgg agtacgagat
    25981 cacgaggaca cagtgaaccg cgtcgccgac ggccttctcg gcgaccccgc cgacgaacga
    26041 cgaaacccca ctcgtagagc ggggttcgcg tagtaccgcc acggggattc gaactggccg
    26101 catgggtagg agtgacgccg ctgatagagc agaatcgcca ctgccggcgt agcgccatca
    26161 aaagtcggac tgcggctcac tttcttcgag ttccctccgg agggctcgga attgtcccgt
    26221 ggacgtcccg cagctagtca cgcaccatcc gtcgcgggtg acaggctttg aagctccatg
    26281 atgggacaac gcggtcgcaa cgacgctttt gagcgcgtag gccttcaaca catgggggcg
    26341 ggatgaacga cgatgactcg gcgctcgagc gctccgtcga ggaggcgatg gcgcagcaag
    26401 agttggacgc cgtccgtgag cttctagcga ccacgtccgg tctcttcccc cacgcaagaa
    26461 ccccagatga aatgcaacgt ttggttgacc ggctcgaaac cgaagagggg cagcgtgatg
    26521 agctcgagcg gatcaagcgc atcgtcgagg cgacgccgca gcttcgcgag gtgttcgaga
    26581 gagtggtctc attccagcgg ttccatgagc gaccgtagcc ggcggttgat gagacgcgag
    26641 tacgcacgtc atgcgcttct ggcctagacg ccctaccccg gtcgcgactg gcgtctctct
    26701 cgacgacgac ggggtgcttc ttgacgccga tgcggccctt gaggatgagc gcgatgacgg
    26761 cctcgaccga gtcctcttct cggaccgatt ggcgcaccag atcaagcggg cacgcgccaa
    26821 aacggggctt gttgtcgccc tcataggacc gtggggcagc gggaagacca ctgtgctcgg
    26881 ctgggtacaa aagcggctgg aggaggggca gccgaaggat gacccgagcc gggtggtcgt
    26941 tcgcttcaac ccttggcttg taagcggcag cgagcagctt gtgtctcagt ttgtatcgga
    27001 gcttgccgtg gccctaccga gaagggccgg agagcgtcgg gcggccgacg cagctgcgcg
    27061 tctccatcga tacgcccggg ccctgcagat tttggagggc gcgccgggcg tgggatggat
    27121 gttcgggaca ggaggagccc ttctgaacga gatcggacga cggaggcacg agacccctga
    27181 gaccgtcttc gagcagcgag agcaagtttg caaagcgctt gaggcgctcg gactccacgt
    27241 agtggttgtg atcgacgacg tagaccgcct cgaggacact gagattcgag atctcatgcg
    27301 gatggtcaag ctggtaggcg actttccgaa cgtgacctac ctcttggcct tcgatcgaga
    27361 gcccgtgctg agcgcgctct cagtcgggca agccgatggc gggcagtacc tcgagaagat
    27421 cgttcagatc gagtaccgct tgccggagcc acggcctgag aaactggatc tgctgctgcg
    27481 agccgggctg aagcgtgaga ttcctgggca cgtacttgag agcgatcggt gggagacggt
    27541 ctacacgaag attgtgctcc cgctcgtctc tcggcctcgt catgttcgtc ggctggcgaa
    27601 cgctttgcca cttgagctag atcaggctgg cgatgaggtc aatccgattg acgtcgtcgc
    27661 cctcacggct ctccaagtac ttcttccgaa gttctatgac gaactgcgac agctgaccga
    27721 ggatctaaca ccccggttta cacagttctt gggttggacg aaagacgaga tcaagcaggc
    27781 cagcgcggcg cgtctaacgc aagcagccga gcgctctgga aatcctgatg tcgcgcgcac
    27841 ggtctacgag ctgctcttcc ctgcggctca ggcggtgcaa tcagatgtgt actactcgga
    27901 tgagccgagc gagtggcgag cggatcaacg agtagcggag cgcgacgtac tagagcgatt
    27961 tttcaccggc acgctgcctg atgacgccgt gacggtggca gacgtgagag ctgcactcga
    28021 ggggttcgcg gagggccccg ttgcgctggc ggccgttttc gattatcagc ctgcagagaa
    28081 gattccagcg ctggtgcaac gagtgcgagc gcaggcccgc gagatcccgg cggaaagcat
    28141 cgtgccgtgt cttccggtcc tcgatgctca gatcgcgcgc atctctgatt tcacggcggg
    28201 actgtcgtcg cctggagact tcgtccgctt cctgattcgg gatctcgttg agcgctttcc
    28261 cgaagggagc gcaagggatt cggtggtcga gcaacgattc gagatggccc cttcccttac
    28321 tgagaagttc acatggctaa ggagcattgg cgcgtgggag gacgggggcg ggaacttcgc
    28381 aagccccgag acttcgcaaa agctccgcga gcggctagcc ggcgaggtgc ttgaggcggg
    28441 tgtggagact cttcttaagg aggagcatcc ggcatggctg ctgcggacag cggaggatga
    28501 tggtgggtcc gacgcctcgg atcgcgtagg cacgttgata ggggacggca ggcttcttcc
    28561 cgcctatctg ggtgggtttc tcgtcttgac gacgtctgct gattaccagc tccagttcga
    28621 tgagctggag cgacgccaag gtcgcacccg actcgtcgag gcagccgaac atgtggatct
    28681 gacgagtctg aaggacgagg agcgctggag agtgctccag ctgagggagc attacgacgg
    28741 ccgcggtcca ctcgactcgt agcgcggatg acgctccacg gaggatcgaa cggatcgcga
    28801 tttcgcgaat gtgggatcgt ggcgggcttc aaccaagcgg ccccggcgac gcgcaaacgt
    28861 cccggggccc ggcacaggag gtatcagctc ccatgcaagc cgaggatatg gcttcgcccg
    28921 ttgcgcagga ccaccggatc gacaccgcga tcgtgggcct cttgatcgac tcgccgacgc
    28981 cgtggtccgt tgcggagatc gagcgggaga ttggcgacga gatcgccact gtggacgcac
    29041 tcgctcgtct cgcgcgacag ggcttgatcc accgcttgga cgacttcgtc ttcatcagtc
    29101 gttccgcagc ctgcgcgttc gagctggcgg cgtagaaatc gaggaagggg ctcgtagggg
    29161 acccccctca ttgacgaagt cctacttcgc ggttctcgac cccccgagcc ggtcgtgacg
    29221 acggcgcgca tccctcaccg cgcgcttgcg ttccgtctcg gcagggcgca tacgagccca
    29281 gcgtcgctgg tcacgtactc gcgctcacga ccagtgcgcc tcgcgtcctc gatctctttg
    29341 cactcttcct tctttgtctg tccttcaccg aacttccacg tcatccgtgc tcccatcaac
    29401 tgggtaggaa ggaccaaccc ctggcaagga ccaggggcgg cttggcggct cctattgcgt
    29461 gctcctgagg ggatcagcaa gcggtgccct ggacccgagt tctccgtgtt cccctacggg
    29521 agccgccttc gacaccgtgc ctggctcagc actaccggaa tggggaggag cccttgccct
    29581 gggcgggcgg tctcaccctt gctgcctctc gcgggcagtt gtcgcgtcaa cgagcgctag
    29641 ccgacctcct caacagccgt catgccgtct ccccccgttg ccggtccaga aagctctgga
    29701 gtcggtcggc tgcctcgtcc tctgcgttgg gcaggaggtg gccatagaga tcgagcgtcg
    29761 tggtcaccga cgtatggccc atgtacgtcg agagcgcctt gatgttcacg tcggcggcga
    29821 tcatcagtga ggcaaatgtg tgccgacact cgtggaggcc gatgggccgg aagtccgcat
    29881 ctcgccatgc cttcaaggcg cgacggcgca gtgtggaagg ctcgaacggc acggtctcgc
    29941 tgatcccgaa cacgaggtcc gagccgcgtc gtccagtcgc cagctcgtga gcgcgcagga
    30001 gcttgcgcag atcgggcact aacggcacac ggcggcgagc cgctcgcgtc ttgccctcct
    30061 gatcgccccc cacgtcgtcc cagccgcgct cgacgttgat taccttcttt ccgaagtcga
    30121 ctgcgctcca ccggagcgcg cgcagctcgc cgcgacggag ccccgcgtac agcgccgtcg
    30181 cccacagggc gcagtcgtcc tccggcaaag cgtccagaag ccgctgcgcc tcggcgggcg
    30241 tcgcgatccg gtcgcgacat cgggacgacg ctggccactc gatcccgtcg gtcggatcga
    30301 ctgcgatgag gtcgcgcttc agcgcccgat cgaagatccg ccgcaacgga tcgcaggcgt
    30361 tcttgacggt gctgcctgcc agaccattcg ccatcaagtc atcgaccaag tcctgcacgt
    30421 gccgccgtcg aacgtggctg aggcgaagat ggccgagtgc agggagcacg tgaaggcgca
    30481 ggctgcggtc atagccacgc acgctcgccg gcttgtaggg ctgaccgact cgattgcgga
    30541 tcaaaccgct gcgtgcgccg tcgatgaact cacgggctgc gtcgtcgacg gacgtcttga
    30601 cgtcaccctt gagggttccg tcctcgagcg cgttcgtcgc ctgcacgcgc cacttcttcg
    30661 ccgctgctag cgagccgaag gtccgccgga ggatctctcc gtcccgcatg accttcgcgc
    30721 ggtagctggg tgagcagtcg caccgcttgc cctcatggct ccggcagcgc ttcttgtgcc
    30781 gcagctcgat gccctcgagc ttgcgcctac gatcctggtg catcaggact tacctcctgg
    30841 tgccgggtcg ggggcggtgc cagccgcgcc ccggccattt acttgtcggg ccgatcctac
    30901 gacggcgcgc atctcgcgat ccaagaagcg ctcgagctcg ctcaccggcc agcgttcagc
    30961 cgaaccgacg tagacgctct taacgtgagg ccgcacccac ttgtcgaacg ccttgccttc
    31021 gaggtccaag gcggcagccg cacgctcgcg attcaaggcg agcggcggga tctccgtcct
    31081 cgggcgctcc gaggcgttgc tacggcgggc gctcacaccg cctgcccttc gacgagagca
    31141 ggcggctcag gttcggcggg ttcgcctgag gaggagccgg atgacgaccg agacgaagct
    31201 gttcgtgtgc gacgccgttg cgctggccgt ccagcaccac gtcggcgcgt cgccggaagg
    31261 ctgacccgcg cacgactgcg cgcctcgcaa gctccacgac tccgcgatgt cgctccaagc
    31321 aggcgatggc atcggtcgat cacggcgagc tggtgccgga agcgacgccg gtagaactcc
    31381 tcctcctgcc gctcctcggc agtgggctcg cgatcggcag gcacttcctc gaaaagatcg
    31441 agcgcctcga acgcgctcat caccggagcg tcgtcaacac cgtcgagtgt tcctcgtagc
    31501 gccatgcgtt cgtgggagtc cgtagaccca ggccgaagtt acgccggctc gaggtcgcat
    31561 ggcggcgacg aagtgcagga tcgcagtggt gtccgacaga cggcagggtt cgccgcccct
    31621 accgggtagt cagtcggagt cgacggtagg gaggatcggg tcaaggacgt ctggaacgtc
    31681 gagtccgtac cgcacggcga cctgtgcgac gtggacccct acgactccgc gcagctggcc
    31741 gagcgcctga ttgaacgtcc agccctgaac acccatcatg tcggtcaatt gccagcgctc
    31801 gcgaaggacg cggtccttgt tgacctcgtc gaggaacttg cgacacgacg cacgcatcgc
    31861 gctcaacggc tctgccagct catctgcgat accgcccttg acgaggacat cggtcaggaa
    31921 gcgtcggatt tcttgaactg attcgacgca atgctcgggg acctcgacct cgtacggcga
    31981 gtagagcacg cgacggtcct ccaagaagag cagcagccct tgggcgacct caacatctga
    32041 gcgcgcgggc tcccacgaca tgccgaacac gggcgtgctg aacccgttga cgcgactgag
    32101 aagtgcagag aacttcatct tccaatcctt gcacggcccg ccaggctcaa ctgtcgagcg
    32161 gaaacccgaa cctctcctcg aaatccttcc gaattaggct ctcgaaccgc ttctcccggc
    32221 gctccttgcc agggagtcga tcacggagct tcgccgcggt gcgcctgaac cgtctcttgg
    32281 cgatcccatt gactccgatc gatccgcatt ggagcgctcc atcgagggcc ttctccgtga
    32341 tctccgcgag gcgctttgcg tcgggcgtgc cgggtctgat caagccaccg tgctcgttgc
    32401 gcggggggtc ctctgcgatc tgttgccaga ccctgacggc tgaatagatc tcgaaccagt
    32461 cgtcccactc gatggttcct gcgaaaacgt cggcatgtgt ctcccatccg acgagctcat
    32521 gctttgcaag gtcggccatc tcgtcccagg tgacagcgtg cgtccgaccg tgctcaccag
    32581 cgatatgcag gcgatgcagg ctcggggcga agagtcgcgc agccttcttc gcctcccaac
    32641 cggctcggcg gcgctcgagg tgatggttca gcgcttgatt cagcacaccg ccaacgacga
    32701 cgccaccaag cgtgaatagg ccggtgatca ctgcggaatc cacgacgcgc aggctacgcc
    32761 ttcccgaccg aaatccagcg tcgttgtccc gccagtcgtc ccaagagcac tcccagaacg
    32821 caaaaacccg ctcgtagagc gggtttgagc agtaccgcca cggggattcg aaccccggtc
    32881 tccgccgtga gagggcggta tcctagtccc ctagacgatg gcggctcggg gtgctaccgg
    32941 ccgacgattg tacaagcagg ccggcggact gtcttgacgc ttggcgggcg aggccgaagc
    33001 cccgcccgcc gacgccccat tccctacggc ttgaccgcga tggcgtcgat ctcgaccggc
    33061 gcgcgaccga ggctcgtgac ccgcagccgg tgctggccgg ccgccaccga gggcgtctcg
    33121 acgagcgtgc ggcggaagcg gccgcggccc ttcaggcgga cgaccttcga ctcgccgtcg
    33181 agcgtcaccc gcagccggcc gcccttcggc agcttccgcg tgacgatcgt ggcgcccgtg
    33241 ccggtgaagc gcagggaggc gctggcgccc ttgcgcgtag cccgcgcgac cgacagcttg
    33301 aacgcgccgt ggcgtcggag cgtcttccaa ccgtccttgg agaagcgcac tcggttggcg
    33361 aggttgtcga ccggcacggt cgacgtcccc gtcgcgtacg ggctgaagtt gcccgcgcgg
    33421 tcgatcgccg ccacgcggaa ctcgtaggtc gtgccgtcac gcgccgggac gcgcgccacg
    33481 gtcttcgccg tgtcaacggc gacctggtgc
//
LOCUS       DATABV010000143         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_686376, whole genome shotgun sequence.
ACCESSION   DATABV010000143 DATABV010000000
VERSION     DATABV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686376"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VHF88_06320"
     CDS             complement(<1..730)
                     /locus_tag="VHF88_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2161418.1"
                     /translation="MTPATATATIPVTDPATGEEIGSIPASGHEEATRAVDAARAALS
                     DWSARDPGDRAACLKAAAGRLRKRLAQIAELETREGGKPIADSRGGVEAGIGAIEQYA
                     ELGPLHAGRSLAGSRDATDMAVREPRGVVALLVPWNDPVAIACQGIAAALAVGNTVVF
                     KPSERTPLSGIALVEALDLPAGVLTLLPGDGRAGAALVESERVDMVVHTGSVDTGRRI
                     AVECAATLGRKALLELGGKDPLIVD"
     gene            complement(772..1479)
                     /locus_tag="VHF88_06325"
     CDS             complement(772..1479)
                     /locus_tag="VHF88_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161419.1"
                     /translation="MRRRDPRRLLRRRRQARARVRIVRVEQSEARPGESVTTRQEADV
                     TLPRAELERIWSAAYLERLARTYWRFLTRISLGLLRVLYSKDAREVVLLMRPFVLLRF
                     HPPEYEATGDRGAVTWRIDRGLLVAPAGRGRGYLRIAVQRPPAGERGGEEVTGRVTSE
                     VANFYPAIAGWGWFARIGRHLYAATQLRIHVIITHAFLRSLARLDLAESRVGALAARL
                     PQAITGDEDPASPAATG"
     gene            1589..2593
                     /locus_tag="VHF88_06330"
     CDS             1589..2593
                     /locus_tag="VHF88_06330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2161420.1"
                     /translation="MPDYDDLRRKFPLRGPLARLDRERDELAKAWLVRLIERASLDEI
                     KELPTDRLADELPELIGDVLTLASAGGRDADLLPPGARERATRLADLRGAREHAAAEM
                     SRDLTAIQAAILGSLAKEPDSLDVEGFADLALRLAEAVGVLQSVAVEGLVEQQVHELE
                     SLANSDPLTGLSNRRYLQDQLRQALGIYKRYETPFALLVLDVDGLKRVNDSKGHQAGD
                     RALVQVATAMRRTLRTTDTAARIGGDEFCVLATNQTAAAAEPLAQRIADAVDTESGAD
                     SPIGISIGVVSCPEHGDDIDALLEMADQAMYRAKAGGLKVALGDPENPELIKVEKTSS
                     "
     gene            2751..>3593
                     /locus_tag="VHF88_06335"
     CDS             2751..>3593
                     /locus_tag="VHF88_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2161421.1"
                     /translation="MSVGDLQELEEVKLLITKGRQIGVLTYAEVATALAEVDLDEGDI
                     EDLHGFIEKSEIELVEEIDPALAAAQQVERAPDKRGRRKKATIDLKPDMTTDSLQLFL
                     KDIGKVRLLTAQEEVDLAKRIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLDLI
                     QEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLNKI
                     GRAERKLVTELGREPTPEEIAEVTGIDPEEVDSIKRSAQAPVSLEKPVGDEEESEFGQ
                     FIADE"
BASE COUNT          573 a         1302 c         1252 g          466 t
ORIGIN      
        1 cgtcgacgat caacgggtcc ttgccgccga gctcgagcag cgccttgcgc ccgagcgtcg
       61 ccgcgcactc gacggcgatc cggcggccgg tgtcgaccga gccggtgtgg acgaccatgt
      121 cgacgcgctc gctctcgacc aacgccgcgc cggcgcgccc gtcgccgggc agcagcgtca
      181 gcaccccggc cggcaggtcg agcgcctcga cgagcgcgat gcccgagagc ggcgtccgct
      241 ccgacggctt gaagacgacg gtgttgccca cggccaaggc ggctgcgatc ccctggcagg
      301 cgatcgcgac cgggtcgttc cagggcacga gcagcgccac gacgccgcgc ggctcacgga
      361 ccgccatgtc ggtcgcgtcg cgcgagccgg caagactgcg tccggcgtgc agcggaccca
      421 gctcggcgta ctgctcgatc gcgccgatgc ccgcctcgac gccgccgcgc gaatcggcga
      481 tcggcttgcc gccctcgcgc gtctcgagct cggcgatctg cgcgaggcgc ttgcgcagcc
      541 ggcccgcggc cgccttcaag cacgcggccc ggtcgccagg gtcgcgcgcg gaccagtcgg
      601 agagggccgc tcgggcggcg tccaccgccc gagtggcctc ctcatggccg gaggccggga
      661 tcgatccgat ctcctcgccg gtcgccgggt ccgtgaccgg gatcgtcgcg gtcgcagtcg
      721 ccggggtcat cgccaatcgg cttccccgcg gtcgccgggc tgaaaccagc gctagcccgt
      781 agcggcgggc gaggccgggt cctcgtcgcc ggtgatcgcc tggggcagcc gggcggccag
      841 cgctccgacc cggctctcgg ccaggtcgag ccgcgccagg ctgcgcagga acgcgtgggt
      901 gatgatcacg tggatccgca gctgggtcgc ggcgtagagg tggcggccga tccgcgcgaa
      961 ccagccccag cctgcgatcg cgggatagaa gttcgcgacc tcggacgtca cgcgcccggt
     1021 gacctcctcg ccgccgcgct cgcccgccgg cggccgctgg accgcgatgc gcaggtagcc
     1081 gcgaccgcga cccgcgggcg cgacgagcag gccgcgatcg atccgccagg tgacagcccc
     1141 gcggtcgccc gtcgcttcgt actccggcgg gtggaagcgc agcagcacga acggccgcat
     1201 cagcagcacc acctcgcgcg catccttgga gtagaggacc cgcagcagac cgagcgagat
     1261 ccgtgtcagg aagcgccagt aggtgcgcgc caggcgctcc aggtaggcgg cgctccagat
     1321 ccgctcgagc tcggcgcgcg gcaacgtgac gtcggcctcc tggcgcgtcg tgacgctctc
     1381 accgggccgg gcctcggact gctcgacgcg gacgatgcgc acccgcgcgc gggcctgacg
     1441 gcggcgacgc agcagccgcc tcgggtcacg tcgcctcatg cggcgcccaa agatacccgc
     1501 cgccaaagct gcgcaaagtc gcgtggcaaa gcgcattccc gccgaagttg gtcttacagt
     1561 ggcctcaggc tcccgaaaag ccgctctcat gcccgactac gacgatctcc gccgcaaatt
     1621 cccgctccgc ggaccgctcg cgcggctgga ccgtgagcgc gacgagctcg cgaaggcctg
     1681 gctcgtccgc ctgatcgagc gggcctcgct cgacgagatc aaggagctgc cgaccgaccg
     1741 gcttgccgac gagctgcccg agctgatcgg cgacgtcctg acgctggcgt ccgccggcgg
     1801 cagggacgcc gacctgctgc ccccgggggc gcgcgagcga gcgacccggc tcgccgacct
     1861 gcgcggggcg cgcgagcacg ccgccgcgga gatgtcgcgc gacctcacgg cgatccaggc
     1921 ggcgatcctc ggcagcctgg cgaaggagcc cgacagcctc gacgtcgagg gcttcgccga
     1981 cctcgcgctg cgccttgcgg aggccgtggg cgtcctgcaa tcggtcgcgg tcgagggcct
     2041 cgtcgagcag caggtccacg agctcgagtc gctcgccaac agcgacccgc tcaccgggct
     2101 ctccaaccgc cgctatctgc aggaccagct gcgtcaggcg ctggggatct acaagcgcta
     2161 cgagacgccg ttcgcgctgc tggtgctcga cgtcgacggc ctcaagcggg tcaacgactc
     2221 caagggccat caggccggcg atcgcgcact cgtccaggtc gcaacggcga tgcggcggac
     2281 gctgcgcacc accgacaccg ccgcgcggat cggcggcgat gagttctgcg tgctggcgac
     2341 gaaccagacc gccgcggccg ccgagccgct cgcccagcgc atcgccgacg ctgtcgacac
     2401 ggagagcggg gccgactcgc cgatcggaat ctcgatcggc gtcgtctcct gtcccgagca
     2461 cggcgacgac atcgacgccc tgctcgagat ggccgaccag gcgatgtacc gggccaaggc
     2521 gggcgggctg aaggtcgcac tcggggatcc tgagaacccc gagttgatca aggtcgagaa
     2581 gacctcgagc tagctcgaca gaccgcgctg tacccaatcc cgggtgctgg agtagtaggt
     2641 gagctctagc gggaatttga ctcgccacgg cgcgccgcta tgcttgcgag cgtctgtttg
     2701 aatggcccga ggcgcggccc tcggacccac cacaagggtg ttgatactgc atgtcggtag
     2761 gggatctcca ggagctcgaa gaggtaaagc tgctcatcac caaggggcgg cagatcggcg
     2821 tgctgaccta cgccgaggtc gccaccgcgc tagcggaggt cgacctcgac gagggtgaca
     2881 tcgaggacct ccacgggttc atcgagaagt ccgagatcga gctggtcgag gagatcgatc
     2941 ccgctctcgc agccgcgcag caggtcgagc gcgcgccgga caagcgcggc cgtcgcaaga
     3001 aggcgacgat cgacctcaag cccgacatga cgacggacag cctgcagctg ttcctcaagg
     3061 acatcggcaa ggtccgcctg ctgaccgccc aggaggaggt cgacctcgcc aagcggatcg
     3121 agcgcggcga cctcgacgcc aagcagaaga tggtcgagtc gaacctccgc ctcgtggtct
     3181 cgatcgccaa gaactaccgc aaccagggcc tgccgttcct cgacctgatc caggagggaa
     3241 cgctcggcct cgtccgcgcc gccgagaagt tcgactaccg caagggcttc aagttctcga
     3301 cctacgccac ctggtggatc cgtcaggcga tcgcccgtgc gctcgccgac aaggcgcgca
     3361 cgatccgcat ccccgtgcac gtcgtcgaga agctgaacaa gatcggccgt gccgagcgca
     3421 agctcgtcac cgagctcggc cgcgagccga cccccgagga gatcgccgag gtcaccggca
     3481 tcgaccccga ggaggtcgac tcgatcaagc gctcggcgca ggcgccggtc tcgctcgaga
     3541 agccggtcgg cgacgaggag gagtccgagt tcggccagtt catcgccgac gag
//
LOCUS       DATABV010000144        10995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_606098, whole genome shotgun sequence.
ACCESSION   DATABV010000144 DATABV010000000
VERSION     DATABV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606098"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VHF88_06340"
     CDS             complement(<1..1116)
                     /locus_tag="VHF88_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161422.1"
                     /translation="MVFDLTDEQAAIQRLARDFAQQEVKPVAEELDREKRFPYEIVSK
                     LGELGLMGIPYPEEYGGGGADTLSYALVVEELARIDSSVAITLAAHTSLGSWPIHAFG
                     NEEQKREWMPRLTAGESLGAFGLTEPEAGSDAGNVRTTASLDGGEWKIDGAKQFITNA
                     GTDISAFVTITARTGEGEVSNIIVPRGTAGYAVGPAYRKMGWNASDTRPLAFDDCRVP
                     EENLLGPRGMGFKQFLQTLDGGRISVAAMGVGLAQGALDEALAYAKERQAFGKPIAKY
                     QAIQMKLADLSTEIEAARLLTYKAAIEKDLGRNFSLTAAQAKLKTGRLAVRATEEAVQ
                     IHGGYGFIEEYPVCRFYRDAKILTIGEGTDEVQQMVIA"
     gene            1304..1681
                     /locus_tag="VHF88_06345"
     CDS             1304..1681
                     /locus_tag="VHF88_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161423.1"
                     /translation="MKSTNWEGAMSATTTIQVPAELVDAICRSLIDQRRDAERREEID
                     GLLEQLADPTTVGDPSYPLSGSRRLLWSAVYDTLCAAAEQLAEDCNEYWRGGIAADDL
                     RAAIVRVDSRYELLLDLGPPPGS"
     gene            complement(1678..2334)
                     /locus_tag="VHF88_06350"
     CDS             complement(1678..2334)
                     /locus_tag="VHF88_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2161424.1"
                     /translation="METARHDPEKLPRGSHGLPPEFVVHSQRERLFAGMARVMAERGY
                     AATTVDQIAAESGVSRKALYTHFSGKEDVLLQAHRSLVQRIAAGAGPVVRDQDDWRSA
                     LRALLDWGLELFAREPALANLSLVEVAAATPASRRLQRETLAKIRALIERATADGPRS
                     VPDVAVDGMLGGMVYVVAKAAEEGDASRLPALRPELMAWFRLVFEGPEAAEHELKPDR
                     "
     gene            2494..3351
                     /locus_tag="VHF88_06355"
     CDS             2494..3351
                     /locus_tag="VHF88_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161425.1"
                     /translation="MTSERGQASVEWVALVLLVALATGGLLAAVPGVDGRSLGGALAH
                     RLTCAVKGGCADNDALVRAYGARDAELVRRNLQGLVFEPGERQIPVDWRDCRAVPCAV
                     APDDRDLDVHRSAAGHRATVFTRLLRRGDRRYIQYWFYYPDSNTTWAGSDKIWRSAPL
                     GQIGQLVTGSERYPGYHPDDWEGAMVRVEADGRTAVRVTSHGHWQWCKRGCEDRWGPA
                     TGWTRVSRGSHAGHVPLDRARERTITADGIRLVPLESIDRSGYRRLDPGISPPWEKDA
                     YENPESSES"
     gene            3403..3564
                     /locus_tag="VHF88_06360"
     CDS             3403..3564
                     /locus_tag="VHF88_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161426.1"
                     /translation="MARTVREQQDKRREEKLKQVQQQVEEGSLVIRKMTTAERKANPP
                     RPPREKKKR"
     gene            3644..5284
                     /locus_tag="VHF88_06365"
     CDS             3644..5284
                     /locus_tag="VHF88_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX2161427.1"
                     /translation="MRAGKPTEPGQGVLDALVYKAGGVKVLADAGIVRPVRPDKLLAV
                     AKTLRRWGRGPASGYITSALLCPGQTAILDDLGSLTFADVDRRTNALANSLAATGVRA
                     GDGVGIMCRNHRGFVEATVAAAKLGADVLYLNTAFAGPQLAEVVEREGPTALIYDEEF
                     SGLLDRAGDGRMRFVAWNEGAAADPTLEELISAGADRLPPPPDRIGRTVILTSGTTGS
                     PKGANRPSPQSLDPVISLLSKFPLKRGQKTVIAAPLFHTWGFAHFALGLMLNSTLVLQ
                     RRFDPEATLAAIARTRADSLVVVPVMMQRILDLPRETRRRYDTSSLSVVAASGSALPG
                     DLATDWMDEYGDNLYNLYGSTEVAQATIATPADMRAAPGTAGSPPRGTVVRLYDEQGR
                     PVPQGGTGRIFVGNELQFEGYTGGGTKEEIDGLMATGDVGRFDAAGRLFVEGRDDEMI
                     VSGGENVFPKEVEDLLARHEAIAEAAALGVPDAEFGQRLLAFVVLKPGAEVGEEDLKA
                     YVKQNLARYKVPREIRFLDELPRNATGKVLKRSLAETA"
     gene            complement(5256..6410)
                     /locus_tag="VHF88_06370"
     CDS             complement(5256..6410)
                     /locus_tag="VHF88_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2161428.1"
                     /translation="MRNPGHLAVGSWSGGRFMHFGEPIDESRLEALLRPGGGIDTVLT
                     ADVYGQGAADRLVGRALAGVPREDYCLVGAIGHDFYSGERQGAKGFPRFSDPALRGPD
                     GYADYLRMATERSLERCGVDRFDVLLLHNPDRTGYTSETVWDAMAALRDEGLTGAIGV
                     APGPANGFVLDLIDCLQRFGDRIDWAMTILGPLEPWPGELALPAAVEARVNVITRVVD
                     YGGLLWGGLRDAGGLGERDHRSFRPKGWIEAGRERIERLRPIAERHGLTPLQLACQWN
                     LAHDAVACVVPTLIQEWVADAQPIENQRAELASTPAEVLLTTGEVNEIRAIGDNFGTM
                     ALKGGVPNHDGEQRPDRWPLTAELVVAGERWGIDPERDLALLTPSPRATA"
     gene            complement(6437..7009)
                     /locus_tag="VHF88_06375"
     CDS             complement(6437..7009)
                     /locus_tag="VHF88_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161429.1"
                     /translation="MRRPALVLAALLAAAVTACGAPSELPEADGVELELARDRVIAAI
                     DTEQRLSASPDAAERLRSRVREIVSSGALEARQLDEFGLAALGELGLAVPSLVVFDRR
                     GVPRELDRAALTAFLAHATTDPAAAMRPAAASAVTQVVEVFERADASSDSEIPTVEMT
                     ADVYLADLAARLREVWPELAGELGAVRSRL"
     gene            complement(7010..8455)
                     /locus_tag="VHF88_06380"
     CDS             complement(7010..8455)
                     /locus_tag="VHF88_06380"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX2161430.1"
                     /translation="MREIAVTVRSGPPEETSADTRVLGLFDGEHLPEPELQQLVDSGE
                     AKPALRKVAVAHEETSGGGRRRVLIAGLGSRDDFDGEAARVAAAAVAGRAKELGASSL
                     SWALPDDGAEVGLVEGTLLRLYSFDRFKSKGDDDSGGVETLEIAGPGVSEEAARRGRI
                     AADAANRARELQNLPSNVADPVFLAGRAQEIADAHESVELELFDRDQIAQRGMGAFTA
                     VARGTDAEPRLIVLRYSGGDRGPHLGFVGKAVTFDTGGISLKPGAKMAEMKFDMSGGA
                     AVLESIDAIAKLALPVRITAVVPATENMPSGHATKPGDIVTALNGKTIEINNTDAEGR
                     LILADALSYAVEQGVERIVDLATLTGAIIVALGSTHAGLFSNHDEWFEQVDAACQATG
                     ELGWRLPLHPETAKLIEGTYADLDNAPAGRKASSITAAHFLSNFVGDVPWVHMDIAGT
                     AWELGRPYVGKGASGFGVRTLIELADRAADA"
     gene            8556..9737
                     /locus_tag="VHF88_06385"
     CDS             8556..9737
                     /locus_tag="VHF88_06385"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX2161431.1"
                     /translation="MPKTVILGTARTPFGKMGGALASFDATDLGGHAIENALERAEVS
                     GDQVQHVVVGQVLQAGQGQIPSRQAQIKGGIPKEVSSETVNKVCASGIRAAGIVDQQV
                     RAGDLDVAVAAGMESMSSAPYLLPKARFGYRMGDAKALDAMVHDGLTNPFSGKQMFVE
                     ATEVGDELEITRPDMDRFALRSHELAIEATDEGRLPEEIAPITVKSRKGDTVVEVDEA
                     PRRGSSIEALAKLPGLTGPDGSHTAGNAPGVNDGAGAIVLASDEWAKANGRTPLATIV
                     GQAAVADDFPYLAKTPANATKKVLKQAGLTVDDIDLFEINEAFASVAISSVRMLGIDE
                     SKVNVNGGAIALGHPIGASGARILGALVHELRRRGGGLGVAAICSGGGQGDAMILEVA
                     A"
     gene            9740..10171
                     /locus_tag="VHF88_06390"
     CDS             9740..10171
                     /locus_tag="VHF88_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX2161432.1"
                     /translation="MAPDTETAQERPATGRKIRVVVAKPGLDGHDRGAKIIARALRDA
                     GMEVIYTGLHQTPEQIVETVIQEDADAVGLSILSGAHMTLVPRIAELLAQQGIEDVVL
                     VVGGTIPADDIPQLKDLGVSEVFTPGAPVREIVEFIENSVD"
     gene            10201..10995
                     /locus_tag="VHF88_06395"
     CDS             10201..10995
                     /locus_tag="VHF88_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX2161433.1"
                     /translation="MKICVLVKEVPDAAVEKRIDPTSKRLDRSGEKNLNPFDTHAIEA
                     AMQLKEGGAVEVEEVVAVTMGPESAFRALHKAVSLGADRSVHLTDDALAGSDVAATGY
                     ALSKVLETESPDLVLLGQQSDDGECYTIGAVVADHLKMPSLTQVIKMDVEGDKLRCER
                     QAEYGYDTVEVSLPAVISVGDAINEPRYPSLKAIMGAKKKPLETKSTGDAGIEAGRVG
                     QDGSRTAVLAINPPPAKEAGEIIEDEDTNETVEKIVNWLDERKLLA"
BASE COUNT         1726 a         3834 c         3800 g         1635 t
ORIGIN      
        1 ggcgatgacc atctgctgaa cctcgtcggt gccctcgccg atggtgagga tcttcgcgtc
       61 gcggtagaag cgacagacgg ggtactcctc gatgaagccg tagccgccgt ggatctggac
      121 ggcctcctcg gtggcgcgca ccgcgagccg accggtcttc agcttggcct gggcggcggt
      181 cagcgagaag ttgcggccga ggtccttctc gatcgcggcc ttgtaggtca gcaggcgcgc
      241 ggcctcgatc tcggtcgata ggtcggcgag cttcatctgg atcgcctggt acttcgcgat
      301 cggcttgccg aaggcctggc gctccttcgc gtaggcgagc gcctcgtcga gcgcgccttg
      361 ggcgagtccg acgcccatcg ccgcgactga gatccggccg ccgtcgagcg tctgcaggaa
      421 ctgtttgaag cccatgccgc gcggcccgag caggttctcc tccggtacgc ggcagtcgtc
      481 gaaggccagc ggtcgcgtgt cagaggcgtt ccagcccatc ttgcggtagg ccgggccgac
      541 cgcgtagccc gcagtgccgc gcggcacgat gatgttcgag acctcgccct cgccggtgcg
      601 tgcggtgatc gttacgaaag cggagatgtc ggtccccgcg ttggtgatga actgcttcgc
      661 gccgtcgatc ttccactcgc cgccgtcgag gctggccgtg gtccgcacgt tgcccgcgtc
      721 cgagccggcc tccggctcgg tcagtccgaa ggcgcctagc gactctccag ccgtgaggcg
      781 gggcatccac tcgcgtttct gctcctcgtt gccgaacgcg tggatcggcc acgagccgag
      841 cgacgtgtgg gccgccaggg tgatcgcgac cgacgagtcg atccgagcca gctcctcgac
      901 gaccagcgca taggagaggg tgtcggctcc gccgccgccg tactcctcgg gataggggat
      961 ccccatcagg ccgagctcgc cgagcttcga gacgatctcg taggggaacc gcttctcgcg
     1021 gtccagctcc tcggcgaccg gcttgacctc ttgctgggcg aagtctcgcg ccagccgctg
     1081 gatcgccgcc tgctcgtcgg tgaggtcgaa gaccacggcg cgggactgta tcgccaagcc
     1141 tggcgccggc gcgcgcctaa tcggccgcac cgggcgctga cagctacctg gcaccgcata
     1201 tatgctaaga aacgataacg attgcaacag gtctgcaacc gctcgtctgg aacacccgtt
     1261 ttgccgtccg gtcgtccagt cacgaacgta tgttcgtagt ccaatgaaga gcacgaattg
     1321 ggagggcgcg atgtccgcaa caacgacgat ccaagttccg gccgagcttg tcgacgcgat
     1381 ctgccgcagc ctgatcgatc agcgtcgcga tgccgagcgc cgggaggaga tcgacggcct
     1441 gctcgagcag ctggccgatc ccaccacggt cggcgatccg tcgtacccac tgagcggctc
     1501 tcggcggctg ctctggagcg ccgtctatga cacgctctgc gcggctgccg agcaacttgc
     1561 cgaagactgc aacgagtatt ggcgcggagg catcgcggca gatgacctcc gagccgcgat
     1621 cgtcagggtc gacagtcgat acgagctgct gctggacctc ggacctcctc caggcagcta
     1681 acggtctggc ttcagctcat gctccgcggc ttccggcccc tcgaacacca gcctgaacca
     1741 cgccatcagc tctgggcgca gcgccggcaa ccgtgacgcg tcgccctcct ccgccgcctt
     1801 cgcgaccaca tacaccatcc cgccgagcat gccgtcgacc gcgacatcgg ggacgcttcg
     1861 gggcccatcg gcagtcgcac gctcgatcag cgcgcgtatc ttcgccagcg tctctcgctg
     1921 tagccgccga gacgccggcg tagcggccgc gacctcgacc agcgagaggt tcgcaagtgc
     1981 gggttcccga gcaaagagct cgaggcccca gtccagcagc gctcgcagtg cggagcgcca
     2041 gtcgtcctga tcacggacca ccggcccggc tcccgcggcg atccgctgga ccagcgacct
     2101 gtgcgcctgc agcaggacat cctccttgcc tgagaagtgg gtgtagagcg cctttctcga
     2161 cacgcccgac tcggcagcga tctggtcgac ggtcgtggcg gcgtagccgc gctccgccat
     2221 gacgcgcgcc attccggcga acagccgctc ccgctgggag tggacgacga actccggcgg
     2281 tagcccgtga ctgccacggg gcagcttctc ggggtcatga cgcgccgtct ccatcctcca
     2341 gcgaacctag cgcggccgca tcgaccaacc ggaactgcct tgtcttagac aacaagaaac
     2401 gtgaatgtgt catccacagt tcgtgcccga actcgcttgg tctcgcaacg ctggcgttgt
     2461 ctcctgtaac cgccatcccg cgatgatcgg ccgatgacga gcgagcgtgg tcaggcctcg
     2521 gtcgaatggg tggcgctggt cttgctcgtc gcgcttgcga caggcggtct gctggcggcg
     2581 gttcccggcg tcgacgggag gtctttgggc ggtgcgttgg cgcaccgcct cacctgcgcc
     2641 gtcaaaggcg gttgcgccga caacgacgcg ctggtgcgcg cgtacggcgc ccgcgacgcc
     2701 gagctagtcc ggcgcaatct tcagggcctg gtcttcgagc cgggcgaacg gcagatcccc
     2761 gtcgactgga gagactgccg cgccgtcccg tgcgccgttg cgcctgacga tcgtgacctc
     2821 gacgtccacc gcagcgcggc cggccaccgg gcgacggtgt tcactcgcct gctgcgccgc
     2881 ggcgatcgcc gctacatcca gtactggttc tactaccccg actcgaacac gacctgggct
     2941 ggctccgaca agatctggag gagcgcgccg ttgggccaga tcgggcagct ggtgaccgga
     3001 tccgagcgct accccggata ccaccctgac gattgggagg gtgcgatggt ccgcgtcgag
     3061 gcggacggcc gcacagccgt acgtgtcacc tctcacggtc actggcagtg gtgcaagcgg
     3121 ggatgcgagg accgctgggg gccagccacc ggttggacgc gcgtgtcgcg cggcagccat
     3181 gccgggcacg tccccctcga tcgtgcgcgt gagcgcacca tcacggcgga cgggattcgc
     3241 ctcgtgccgt tggagagcat cgaccgaagc ggctaccggc gactcgatcc agggatctcg
     3301 cctccgtggg agaaggacgc atacgagaat ccggagagct ccgagtcctg agcggctacc
     3361 tttgaagatg acgctggcta tcgaaagcga ggaactcccg ctatggcgag aaccgtgcgc
     3421 gagcagcagg acaagcgacg cgaggagaag ctgaagcagg ttcagcagca ggtcgaggag
     3481 ggctcgctcg tgattcgcaa gatgacgacc gccgagcgca aggccaatcc accgcgccca
     3541 ccccgggaga agaagaaacg ctgatcggtc gctgacgcgc aggcggaccc ggaaggcggg
     3601 gtagcgccac gtctcgttcg ggcctggcac actccgccgg cggatgcggg cggggaagcc
     3661 gacagagccg ggtcaaggag ttctagacgc gcttgtctac aaggccggcg gcgtcaaggt
     3721 gctggcggac gcaggcatcg tgcggccggt acggcccgac aagctgctcg ccgtcgccaa
     3781 gacgctcagg cgctggggac gcgggcccgc gtccggctac atcacctcgg cgctcctatg
     3841 ccccggacag acggcgatcc tcgacgatct cgggtcgctc accttcgccg atgtcgaccg
     3901 ccgcaccaac gccctcgcca actcactcgc agcgaccggc gtgcgcgccg gcgacggcgt
     3961 cggaatcatg tgtcgtaacc accgcgggtt cgtcgaggcg actgtcgccg ccgcaaagct
     4021 cggtgccgat gtgctctacc tcaacaccgc cttcgccggt cctcaactcg ccgaggtggt
     4081 cgagcgcgag ggtccgactg cgctgatcta cgacgaagag ttcagcggac tgctcgaccg
     4141 agccggcgac ggccggatgc gtttcgtcgc ctggaacgaa ggagctgccg ccgatccgac
     4201 gctcgaggag ctcatctccg ccggggccga ccggctgccc ccgccgccag accgcatcgg
     4261 tcggacggtg atcctgacgt ccggcaccac cggctcgccg aagggcgcca accgcccgtc
     4321 gccgcagtca ctcgatccgg tcatctcgct gctctcgaag ttcccgctca agcgtggcca
     4381 gaagaccgtc attgccgctc cgctgttcca cacctggggc ttcgcgcact tcgcgctcgg
     4441 gttgatgctc aactcgacgt tggtgctgca gcggcgcttc gacccggagg caacgctggc
     4501 ggcgatcgcg cgcacgcgcg ccgactcgct cgtcgtggtg ccggtgatga tgcagcgcat
     4561 cctcgacctg ccccgcgaga ctcggcgccg ctacgacacc tcttcgctgt cggtcgtcgc
     4621 ggcgagcggc tcggcgctgc ccggtgatct tgcgaccgac tggatggacg agtacggcga
     4681 caacctctac aacctctacg gctcgaccga ggtcgcgcaa gcgaccatcg caacacccgc
     4741 cgacatgcgc gcggcgcccg ggaccgcggg cagccccccg cgcgggacgg tcgtgcggct
     4801 ctacgacgag cagggtcggc cggtgccaca gggggggacg ggtcgcatct tcgtcggcaa
     4861 cgagctgcag ttcgagggct acacgggcgg cgggacgaag gaggagatcg acggcttgat
     4921 ggccactggc gacgtcgggc gcttcgacgc cgccggacgc ctcttcgtcg agggccgcga
     4981 cgacgagatg atcgtctcgg gaggggagaa cgtcttcccg aaggaggtcg aggacctgct
     5041 tgcgcgccac gaggcgatcg ccgaggccgc ggcgcttgga gtgccggatg ccgagttcgg
     5101 ccagcggctg ctcgcgttcg tcgtgctcaa gcccggcgcg gaggtcggtg aggaggacct
     5161 caaggcgtat gtgaagcaga acctcgcccg ctacaaggtg ccgcgcgaga tccgcttcct
     5221 cgacgagctg ccgcgcaacg cgaccggcaa ggtgctcaag cggtcgctcg cggagacggc
     5281 gtaagcagcg ccaggtcgcg ctcggggtcg atgccccagc gctcgcccgc gacgaccagc
     5341 tcggcggtca gcggccaacg gtccggacgc tgctcgccgt cgtggtttgg cacgccgccc
     5401 ttcagcgcca tcgtcccgaa gttgtcgccg atcgctcgga tctcgttgac ctcgccggtc
     5461 gtcagcagga cctcggccgg ggtcgacgcc agctcggcgc gctggttctc gatcggctgg
     5521 gcgtcagcga cccactcctg gatcagcgtt ggcaccacgc atgcgacggc gtcgtgggcg
     5581 aggttccact ggcacgccaa ctggagcggc gtcagcccgt gtcgctcggc gatcgggcgc
     5641 agccgctcga tccgctcgcg tccggcctcg atccagccct tcggcctgaa ggagcggtgg
     5701 tcacgctcac cgaggccgcc ggcgtcgcgc aggccgcccc acaacagccc gccgtagtcc
     5761 acgacgcggg tgatcacgtt gacgcgcgcc tcgacggccg ccggtagcgc cagctcgccg
     5821 ggccagggct cgagcggccc caggatcgtc atggcccagt cgatccggtc gccgaatcgc
     5881 tgcaggcagt cgatcaggtc gagcacgaag ccgttggcgg ggcccggggc gactccgatc
     5941 gcgccggtca gcccctcgtc gcgcagtgcg gccatcgcgt cccagacggt ctcgctcgtg
     6001 tagccggtgc ggtctgggtt gtgcagcagc aggacgtcga agcggtcgac gccgcagcgc
     6061 tcgaggctgc gctcggtcgc catccgcagg tagtcggcgt agccgtcggg accccgcagc
     6121 gccgggtcgc tgaagcgcgg aaaccccttg gccccctgac gctcgccgga gtagaagtcg
     6181 tgcccgatgg cgccgacgag gcaatagtcc tcccgtggaa ctccggccag cgctcgtccg
     6241 accagcctgt cggcggcacc ctgtccatag acgtcggcgg tcagcacggt gtcgatcccg
     6301 ccgccggggc gcagcagggc ctcaaggcgc gactcgtcga tcggctcgcc gaagtgcatg
     6361 aagcggccgc cgctccatga gccgacggcg aggtgtcccg ggttgcgcat ggttcggatg
     6421 ctacggcgac cggctctcag agcctgctgc ggaccgcacc gagctcaccg gccagctcgg
     6481 gccacacctc gcgtaggcgc gcggcgaggt cggccaggta gacgtcggcc gtcatctcaa
     6541 cggttgggat ctcgctgtcg gagctcgcat ccgcccgttc gaagacctcg acgacctgcg
     6601 tgacggcgct cgccgccgcc ggccgcatgg cagccgcggg gtccgtcgtg gcgtgcgcga
     6661 ggaaggcggt cagggcggcg cggtcgagct cgcgcgggac cccgcgccgg tcgaagacca
     6721 ccaggctggg gacagccagg ccgagctcac cgagcgcggc gagcccgaac tcgtcgagct
     6781 gcctagcctc caaggcgccc gaagacacga tctcgcgcac ccgagagcgc agacgctctg
     6841 cggcgtccgg cgaggcgctc aaccgctgct ccgtgtcgat cgcggcaatc acgcggtcgc
     6901 gcgccagctc gagctcgacg ccgtcggcct cggggagctc gctcggagcc ccgcacgcag
     6961 tgactgccgc cgcgaggagc gcggcgagga cgagcgccgg gcgccgcacc taggcgtcgg
     7021 ccgcgcggtc ggccagctcg atcaaggtgc gcaccccgaa gccggaggct cccttgccga
     7081 cgtatggccg gccgagctcc caggcagtgc ccgcgatgtc catgtggacc cacggcacgt
     7141 cgccgacgaa gttgctcagg aagtgagcag cggtgatcga cgacgccttg cgtccagccg
     7201 gggcgttgtc gaggtcggcg taggtgccct cgatcagctt ggcggtctcg gggtgcagcg
     7261 gcaggcgcca gcccagctcg ccggtcgcct gacaggcggc gtcgacctgc tcgaaccact
     7321 cgtcgtggtt cgaaaacagt ccagcatggg tggagccgag cgcgacgatg atcgcgcccg
     7381 tcagagtggc gaggtcgacg atccgctcga cgccctgctc gacggcatag gagagcgcgt
     7441 cggcgaggat cagccggccc tccgcgtcgg tgttgttgat ctcgatcgtc ttgccgttga
     7501 gcgcggtgac gatgtcgccg ggcttggtcg cgtggccgct cggcatgttc tcggtggcgg
     7561 gcacgacggc cgtgatcctc accggcagcg cgagcttggc gatcgcgtcg atcgactcca
     7621 gcaccgcggc gccgcccgac atgtcgaact tcatctcggc catcttggcc cccggcttca
     7681 gcgagatgcc gcccgtgtcg aaggtgacgg ccttgccgac gaagccgagg tgcgggccgc
     7741 gatcgccgcc gctgtagcgc aggacgatca gccgcggctc ggcgtccgtc ccgcgcgcga
     7801 ccgcggtgaa cgcgcccatt ccgcgctgag cgatctggtc gcggtcgaac agctcgagct
     7861 cgacggactc gtgggcgtcg gcgatctcct gcgcgcggcc ggcgaggaag accgggtcgg
     7921 cgacgttcga cggcaggttc tggagctcgc gcgcgcggtt cgccgcgtcg gcggcgatgc
     7981 gaccgcgccg tgcagcctcc tccgagacgc ccggtccggc gatctcgagc gtctcgacgc
     8041 cgccggagtc gtcgtcgccc ttggacttga agcggtcgaa ggagtagagc ctcagcagcg
     8101 ttccctccac gaggccgacc tcggctccgt cgtcgggcag cgcccaggac aacgaggacg
     8161 cgccgagctc cttcgctcgg ccggcgacgg ccgccgcggc gacgcgggcc gcctcgccgt
     8221 cgaagtcgtc gcgcgacccg aggccggcga tcaggacgcg ccgccgtccg ccgcccgagg
     8281 tctcctcgtg ggcaaccgcg accttgcgca gcgccggctt cgcctcaccg ctgtcgacga
     8341 gctgctgcaa ctcgggctcg ggcaggtgct cgccgtcgaa caggccgagc acgcgggtgt
     8401 cggcgctggt ctcctctggc ggtccggagc ggactgtgac ggcgatctca cgcatgacgg
     8461 gggagcttaa aggcggaggc gcgcagccgg atcggcacta cgcctccgat atggtccaat
     8521 gacccccagg cagaccgaag gagaccgacg cccccatgcc caagaccgtg atcctaggca
     8581 ccgctcgcac cccgttcggc aagatgggag gcgcgctcgc ctcgttcgac gcgaccgatc
     8641 tcggcgggca tgcgatcgag aacgcgctcg agcgtgcgga ggtatccggc gaccaggttc
     8701 agcacgtagt cgtcggacag gtcctccagg ctggtcaggg tcagatcccg tcgcgccagg
     8761 cccagatcaa gggcgggatt cccaaggagg tctcgtcgga gaccgtcaac aaggtctgcg
     8821 cctcggggat ccgcgcggcc ggcatcgtcg atcagcaggt ccgtgccggc gacctcgacg
     8881 tcgccgtcgc agcgggcatg gagtcgatgt cgagcgctcc ctacctcctg cctaaggccc
     8941 gcttcgggta ccgcatgggc gacgccaagg cgctcgacgc gatggtccac gacgggctga
     9001 cgaacccctt ctccggcaag cagatgttcg tcgaggcgac cgaggtcggc gacgagctcg
     9061 agatcacccg tcccgacatg gaccgcttcg cgctgcgcag ccacgagctg gcgatcgagg
     9121 ccaccgacga gggtcgcctg cccgaggaga tcgcgccgat caccgtcaag tcgcgcaagg
     9181 gcgacacggt cgtcgaggtc gacgaggcgc cacgccgtgg cagcagcatc gaggcgctcg
     9241 ccaagctgcc cgggctgacc ggtcccgacg ggtcgcacac cgccggcaac gctcccggcg
     9301 tgaacgatgg cgccggagcg atcgtcctcg ccagcgacga gtgggcgaag gccaacggcc
     9361 gcacgccgct ggcgacgatc gtcggccagg cagccgtcgc cgacgacttc ccctacctcg
     9421 ccaagactcc ggccaacgcg acgaagaagg tgctcaagca ggccgggctg accgtcgacg
     9481 acatcgacct cttcgagatc aacgaggcct tcgcctcggt tgcgatcagc tccgtccgga
     9541 tgctcgggat cgacgagtcg aaggtcaacg tcaacggcgg cgcgatcgcg ctcgggcatc
     9601 cgatcggggc gtccggcgcc cggatcctcg gcgcgctcgt ccacgagctg cgccgccgcg
     9661 gcggcggtct cggcgtcgcc gcgatctgct cgggtggcgg tcagggcgac gcgatgatcc
     9721 tcgaggttgc cgcgtagcga tggcccccga caccgagaca gcacaggagc gcccggcgac
     9781 cggtcgcaag atccgcgtgg tggtcgccaa gccggggctc gacggccacg accgcggcgc
     9841 caagatcatc gcccgcgcgc tgcgcgacgc gggcatggag gtcatctaca cgggtctgca
     9901 tcagacgccc gagcagatcg tcgagacggt gatccaggag gacgccgacg cggtcggcct
     9961 gtcgatcctc tcgggcgccc acatgacgct cgtcccgcgg atcgctgagc tgctcgcgca
    10021 gcaggggatc gaggacgtcg tgctggtcgt cggcggaacg atccccgcgg acgacatccc
    10081 gcagctcaaa gacctcggcg tctctgaggt cttcacgccc ggcgcgccgg tgcgggagat
    10141 cgtcgagttc atcgagaatt ccgtagactg actcgtcagt caactttcca aggagacccc
    10201 ttgaagatct gcgtcctcgt gaaagaggta cccgacgccg ccgtcgagaa gcgcatcgat
    10261 cctacgagca agcgcctcga tcgcagcggt gagaagaacc tgaacccctt cgacacccac
    10321 gccatcgagg ctgcgatgca gctgaaggag ggcggtgcgg tcgaggtcga ggaggtcgtc
    10381 gcggtgacga tgggccccga gagcgccttc cgcgcgctcc acaaggccgt ctcgctcggc
    10441 gccgatcgct cggtccacct gaccgatgac gccctcgccg gctccgacgt cgccgccacc
    10501 ggctacgcgc tcagcaaggt gctcgagacc gagagccccg atctggtcct cctcggccag
    10561 cagtcggacg acggcgagtg ctacacgatc ggcgccgttg tcgccgacca cctgaagatg
    10621 ccgtcgctga cgcaggtgat caagatggac gtcgagggcg acaagctgcg ctgcgagcgc
    10681 caggccgagt acggctacga caccgtcgag gtgtcgctgc cggcggtgat ctccgtcggc
    10741 gacgcgatca acgagccgcg ctacccgtcg ctgaaggcga tcatgggcgc gaagaagaag
    10801 ccgctcgaga cgaagtcgac cggcgacgcc ggcatcgagg ccggacgcgt cggccaggac
    10861 ggctcgcgca ccgcggtcct cgcgatcaac ccgccgcccg cgaaggaggc cggcgagatc
    10921 atcgaggacg aggacaccaa cgagacggtc gagaagatcg tcaactggct cgacgagagg
    10981 aagctgctgg catga
//
LOCUS       DATABV010000145         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1650095, whole genome shotgun sequence.
ACCESSION   DATABV010000145 DATABV010000000
VERSION     DATABV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650095"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VHF88_06400"
     CDS             complement(<1..1138)
                     /locus_tag="VHF88_06400"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX2161434.1"
                     /translation="MPNDSLTVTDNRTGQTYELPIADGTVKAMDFRQIKTSEDDFGLM
                     TYDPAFTNTASCRSAITYIDGEAGVLEHRGIPIEQLCESSSFLEVAYLLVHGELPSKA
                     ALDYWVYAITHHTYVHENIKKSMEAFRYDAHPMAMLLSTVGALSTYYPAAKNIDDPTE
                     RYMAIVRLIAKMPTLAAFSYRHNLGLPYVYPDNDLWYTGNFLSMLYRINEPQYEPDPR
                     LERALDVLWILHADHEQNCSTAAVSSVGSSQVDPYSAVAAGIAALYGPLHGGANEAVL
                     RMLDRIGTVENIPDFLQGVKDRKEKLMGFGHRVYKNYDPRARIIKKHVDEVLDVLGVD
                     NPKVEIAVELEKHALDDEYFTDRKLFPNVDFYSGLIYEAMNIPTD"
     gene            complement(1157..1453)
                     /locus_tag="VHF88_06405"
     CDS             complement(1157..1453)
                     /locus_tag="VHF88_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161435.1"
                     /translation="MAIDDGTAISYTALPTGVPVYSSDEIAVGTVREVLDNARERIFD
                     GIVFENEAGVRVFVDAPEVARTAERGVTLTLTAAQAAELPPYKGGGMLGKLFRR"
     gene            complement(1459..1692)
                     /locus_tag="VHF88_06410"
     CDS             complement(1459..1692)
                     /locus_tag="VHF88_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161436.1"
                     /translation="MEVYYVIGSVLAACALLVSLLGVVRKDFPGSRGGEIAVGVIFLV
                     LVLATVSAAVVGALTNEEEDEPQDHGAVPTLIA"
     gene            complement(1725..1985)
                     /locus_tag="VHF88_06415"
     CDS             complement(1725..1985)
                     /locus_tag="VHF88_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161437.1"
                     /translation="MEAVLWMALVLKIPIFALLWLVWWAAKAPEPADATEDDGGSKQP
                     DRPHGPLSPPRPPRRGPDHGAPLPAPPRVRTRAVGRRSGVGR"
     gene            complement(2033..>2911)
                     /locus_tag="VHF88_06420"
     CDS             complement(2033..>2911)
                     /locus_tag="VHF88_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2161438.1"
                     /translation="TPFGERERMLGGSAVHFSLAASFFTDVRVVGPVGDDFGDAEYEV
                     LHGRGVNTDDIEHVAGGDTFFWRGHYEYDFNQAHTDDTQLNVFGEFEPKLSEASRAAD
                     HLFLANIQPDLQRGVRSQCGGVRWAGLDSMNLWIEIARDSLVETISGVDLVFLNDAEI
                     RQLTEQPNLVRAARAVMEMGPKVVVAKQGEYGAALFTESGFFALPAFPLETVIDPTGA
                     GDSFAGGFLGYLSSQDDWTGEATLRRAMAYGSALASFNVEEFGTERVARLERDEIDAR
                     FEQFRQITTLEAPAPA"
BASE COUNT          433 a          934 c         1071 g          473 t
ORIGIN      
        1 tgtcggtcgg gatgttcatc gcctcgtaga tcaggcccga gtagaagtcg acgttcggga
       61 acagcttgcg gtcggtgaag tactcgtcgt cgagcgcgtg cttctcgagc tcgacggcga
      121 tctcgacctt cgggttgtcg acgccgagca catcgagcac ctcgtcgacg tgcttcttga
      181 tgatccgcgc gcgcgggtcg tagttcttgt agacccggtg gccgaagccc atcagcttct
      241 ccttgcggtc cttcacgccc tggaggaagt cggggatgtt ctcgaccgtc ccgatccggt
      301 cgagcatccg cagcaccgcc tcgttggcgc cgccgtgcag cggtccgtag agcgcggcaa
      361 tcccggcggc caccgccgag taggggtcga cctgcgacga gccgaccgag ctcaccgccg
      421 ccgtcgagca gttctgctcg tggtcggcgt ggaggatcca gagcacgtcg agcgcccgct
      481 cgagccgcgg gtcgggctcg tactgcggct cgttgatgcg gtagagcatc gagaggaagt
      541 tgcccgtgta ccagaggtcg ttgtccggat agacgtaggg caggccgagg ttgtggcggt
      601 aggagaacgc cgcgagcgtc ggcatcttgg cgatcagccg tacgatcgcc atgtagcgct
      661 cggtcgggtc gtcgatgttc ttcgccgccg ggtagtaggt cgacagcgcg ccgaccgtcg
      721 agagcagcat cgccatgggg tgggcgtcgt agcggaacgc ctccatcgac ttcttgatgt
      781 tctcgtggac gtaggtgtgg tgggtgatcg cgtagaccca gtagtcgagc gcggccttgc
      841 tcggcagctc gccgtggacg agtaggtagg cgacctcgag gaagctcgag ctctcgcaga
      901 gctgctcgat cgggatgccg cggtgctcga gcacgcccgc ctcgccgtcg atgtaggtga
      961 tcgcgctgcg acacgacgcc gtgttggtga acgccgggtc gtaggtcatc aacccgaagt
     1021 cgtcctccga cgtcttgatc tggcggaagt ccatcgcctt gacggtgccg tcggcgatcg
     1081 gcagctcgta ggtctgcccg gtgcggttgt cggtgaccgt cagcgagtcg ttcggcacgg
     1141 ccgccacggt agcgatctag cgtcggaaca gcttgccgag catgccgccg cccttgtatg
     1201 gcggaagctc tgctgcctgt gcggcggtga gcgtgagggt cacaccgcgc tcggcggtgc
     1261 gcgcgacctc gggggcgtcg acgaagacgc gcacgccggc ctcgttctcg aagacgatgc
     1321 cgtcgaagat ccgctcgcgc gcgttgtcga gcacctcgcg gaccgtcccg acggcgatct
     1381 cgtcggagct gtagacggga acgccggtcg gcagcgcggt gtagctgatc gctgtgccgt
     1441 cgtcgatcgc cacggcgatc aggcgatcag cgtggggacg gcgccgtggt cctgcggctc
     1501 gtcctcctcc tcgttggtga gcgcacccac caccgcggcg gagaccgtcg cgagcaccag
     1561 cacgaggaag atcacgccga cggcgatctc gccgccgcgc gagccgggga agtccttgcg
     1621 gaccacgccg agcagcgaga cgagcagggc gcaggccgcg agcacgctgc cgatgacgta
     1681 gtagacctcc accgcgggga aaacctaaca cgccccggct ggggtcagcg tccgacgccg
     1741 gaccggcgcc ctaccgcacg agtgcggacg cgcgggggtg cgggcagtgg ggcgccgtgg
     1801 tcgggaccgc ggcggggagg tcgtggcggg gagagcgggc cgtgcgggcg gtcgggctgc
     1861 ttgctgccgc cgtcgtcctc ggtcgcgtcc gccggctcgg gcgccttggc ggcccaccag
     1921 acgagccaca gcagcgcgaa gatcgggatc ttcaggacca acgccatcca gagcactgcc
     1981 tccatggtgt ccgagagtac ccgcccggcg gccggcgaac cggatcgatt tgttaagccg
     2041 gcgcgggcgc ttcgagcgtg gtgatctggc ggaactgctc gaagcgggcg tcgatctcgt
     2101 cgcgctcgag gcgggcgacg cgctcggtgc cgaactcctc gacgttgaac gaggcgagcg
     2161 cggagccgta ggccatcgcg cggcgcagcg tcgcctcgcc ggtccagtcg tcctgggagg
     2221 agaggtagcc gaggaagccg ccggcgaagg agtcgccggc gccggtcggg tcgatcaccg
     2281 tctcaagcgg gaaggcgggc agcgcgaaga agccgctctc ggtgaacagc gcggcgccgt
     2341 actcgccctg cttggcgacg acgaccttcg gtcccatctc catcactgcg cgcgccgcgc
     2401 ggacgaggtt cggctgctcg gtcagctggc ggatctcggc gtcgttgagg aagacgaggt
     2461 cgacgccgga gatcgtctcg accagcgagt cgcgcgcgat ctcgatccag aggttcatcg
     2521 agtcgaggcc cgcccagcgg acgccgccgc actggctgcg cactccacgc tgcaggtccg
     2581 gctggatgtt cgccaggaag aggtggtcgg cggcccgcga ggcctccgac agcttcggct
     2641 cgaactcgcc gaagacgttg agctgggtgt cgtcggtgtg ggcctggttg aagtcgtact
     2701 cgtagtggcc gcgccagaag aaggtatcgc cgccggcgac gtgctcgatg tcgtcggtgt
     2761 tgacgccgcg gccgtgcagc acctcgtact cggcgtcgcc gaagtcgtcg ccgaccggcc
     2821 cgacgacgcg cacgtcggtg aagaagctcg cggcgagcga gaagtggacg gccgagccgc
     2881 cgagcatccg ctcgcgctcg ccgaagggcg t
//
LOCUS       DATABV010000146        11854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2775786, whole genome shotgun sequence.
ACCESSION   DATABV010000146 DATABV010000000
VERSION     DATABV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775786"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VHF88_06425"
     CDS             <1..1208
                     /locus_tag="VHF88_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2161439.1"
                     /translation="AAMERLVGEGKLGAKTGGDGFYSDGQPNVDGDGDADAEELAELW
                     AMKALREALLVLEEGVSSVREIDLGLMAGAGLDPRRGLLPPFWKADVEGLDVALEKME
                     ALEAKHGPERFGVPTILRRMVAQGRLGIAAGQGFYAWARPDDGDQRETVKLETRGDVA
                     IAWLANAPMNAISPQVIEDLGAVWEKVTSEGEVGALVIASSIPVVFSAGADIKAFTQM
                     DEEKGAELIHSGHALLRDLGSNRVSTVAAVNSIAFGGGCELAMACDVRIAAESAIFGQ
                     PEIKLGIIPGFGGTQRLPRLVGESKALEMNLTGDAITAGDAFEHGLVNRVVPDHELFD
                     TALTWARKLAGQAPVAVERIKEVSAAGNLDEGIEAEKAGFAAVFATEDAKEGIGAFLG
                     KRTPKWSGR"
     gene            1193..1957
                     /locus_tag="VHF88_06430"
     CDS             1193..1957
                     /locus_tag="VHF88_06430"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX2161440.1"
                     /translation="MVRTLESTTAELAGLLRGAERAIVLTGAGISVPSGIPDFRSPGT
                     GIWEGVDPMEVAHIDAWRRDPDRFWSFYGSRFSGLVDKRPNRAHEVVAELERSGRVRA
                     VITQNIDRLHRVAGSRRLLEIHGSIESSVCPQCGGRTAVEQVVALLAAGDGAPECPAC
                     IAPLKPDVVLFGELLPADVMAEAEQLALEADLMVCIGSSLTVHPAAGLPQLTLGSGGR
                     IAIVTEGPTPLDDRAAVKLSGDVVAELEAVAEALRT"
     gene            complement(1954..2805)
                     /locus_tag="VHF88_06435"
     CDS             complement(1954..2805)
                     /locus_tag="VHF88_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161441.1"
                     /translation="MASPLFGFVQLEFGFLLGPGDGRYMVRADPGERPETVLVLSTLA
                     APQRRRLRGRRAKAVTEATPEAVPTNRATVVHTDPFPTPDHADLWLAAVRDDSDRRAA
                     ELDRALSTLNGALHAYRVARADPHVRDVDHSQALVTRIGFGSGDEAVAGMFKQALELP
                     AAGIVRAKRSMEAPEERFAALLSGRERVLACEELVLRARMDIAAGRGREAALQARVAL
                     EALLAELSQLPDGRRGELEGDRRAVGDAANAALRGELSAELADELAASVGRMESALRA
                     RRLDSAI"
     gene            complement(2857..5391)
                     /locus_tag="VHF88_06440"
     CDS             complement(2857..5391)
                     /locus_tag="VHF88_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX2161442.1"
                     /translation="MKKRLWHTLLCAGVALTAALTGFTVAASAETRVLTVTLVSGEVV
                     TITVDVPPGTPVSAIQLPSDIGPVQSVTQQEVAPATTTETTPPPPPPVPTATTPPPPP
                     APTTPAPQTTPAPPAAPAPPPAPLETDPAGPAPTPSGGRKQKSGGDDRRAVKRPTTKN
                     SQPSPEGEAHERGGAQAEPPALRNPDGSPSTSNPAFFEALPGPSVAQGVPNFVIRKFR
                     VPLFLLPIYQAAGIEYGIRWEILAAINEIETDYGRNLNVSSAGALGWMQFMPPSWRAY
                     GVDANKDGRKDPYNPVDAIFAAARYLKAANYENDVRGAIFAYNHADWYVDSVLLRARL
                     ISGVPADLVGSLTGLTEGRFPVAARARYADDLAEQEAVKRVRVGENAANVVESQDDRR
                     SLEIFAQKGSPVVATNDGVIKKVGESKRLGNYVVLQDVYGNRYTYAGLGEIAEHYPVL
                     KEDATDVRAIKANGDAVDPEPTKAASAGRQAASDDATDDDAGAATATAEPTASLPFKE
                     RLFAHPNAEGARESGGLEQLLAAKARLDGKFETYSNYFSRPFGADAKDVRLRRLKVGS
                     RVIGGTILGRLGKVDAAKAAHLDFGIRPAGRGAPSIDPKPILDGWKLLEATAIYRAAG
                     KNVLYGRDARVGSLSIGQILLLPKPLLEKRVLADERIEIYEGGRSDIQSGQVDRRVLA
                     TLAYLAESGLRPTVTSLKSGHSYLTASGNVSHHSSGNAVDVAAINGVPIFGHQEPGGV
                     TDQAVRRLMRLQGTMRPDQIISLLDYGQNTVAMGDHHDHIHVGFQPLFGQNRRLGQQA
                     LAVLEPGQWSDLIARLREIDNPVVPTEPSKYSLPADKPASHAHDGE"
     gene            5731..6078
                     /locus_tag="VHF88_06445"
     CDS             5731..6078
                     /locus_tag="VHF88_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161443.1"
                     /translation="MAADREAPFVDRVSQQGEEALGRLAEQLIGNPIVTGAVSRAFEA
                     REKAVQAQEAALGALNIPSAADIERVTRRLRSVSQRLESIEDTLDRLEERLAAPPPKP
                     KPAPKTRSKAKKS"
     gene            complement(6075..6779)
                     /locus_tag="VHF88_06450"
     CDS             complement(6075..6779)
                     /locus_tag="VHF88_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161444.1"
                     /translation="MTDPATLHLRPATTLAERVLLPGDPHRALAVAQALLERPRMFNH
                     NRGLWGYTGTARDGEPLTVQATGMGGPSAAIVVEELIELGARALVRIGTCGALRDGFE
                     LGELVTAATVIGADGTSAALGAESPLAPDRPLTDALVSAGARPVTAVSTDLFYDPRSD
                     LPAEWLASGADVVEMEAATLLTIASRREVAAAVVLGITDLLAGGDRRRLPHHRVEPLG
                     LALGEAGWAALTQSPS"
     gene            6840..7136
                     /locus_tag="VHF88_06455"
     CDS             6840..7136
                     /locus_tag="VHF88_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161445.1"
                     /translation="MPDRPDIQDALRDAVDRTVRATVDTRERAQGAAGGVADAVDDLV
                     KGAERNIARGRRGVLAAVEDRLPASQDDMRALRSELRRIDRRLAEIERRLSNVG"
     gene            complement(7133..9142)
                     /locus_tag="VHF88_06460"
     CDS             complement(7133..9142)
                     /locus_tag="VHF88_06460"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2161446.1"
                     /translation="MNYFVTGATGFIGRHLVEELLKRDGTIYVLVREGSRGKVDALAQ
                     RLNAPEGRIVPVPGDLTQPALGVDEDALPADLTIDHFFHLAAVYDIEADEQTAQRANV
                     EGTRHAIELANRLSPGRFHHVSSIAVAGDYRGVFQEDMLDEGQRLPHHYHRTKYESEQ
                     MVRRDVKAKTLVYRPGIVVGHSQTGEIDKIDGPYYLFTLLKKLRGALPAWMPLAGPEG
                     GKTTIVPVDFVARAIDHIGHMADEDLPGDTYHLVDPEPMTVGAAVNTFARAAHAPQLA
                     MRVDENLTKAVPAPVRAGVMALPTVKKIRNQLYRDLGIPPEAMEVRDFKCTFDARDAQ
                     RALSGTGIAVPPLDSYAGRLWDYWERNLDPALFRDRSLTTAVRDKRIVITGASSGIGL
                     ETAVRVGEAGAIVILVARTRAKLEEVAEQIRAAGGTAHVHPADLSDIDDVERVGREIV
                     DEHGGVDVLVNNAGRSIRRSVARSYDRFHDYERTMQLNYFGALKLIMTFLPGMRERKS
                     GHVINVSSIGVQTNTPRFSAYVASKSALDAFSRCAAPECVADNVKFTTVYMPLVRTPM
                     IAPTDIYKAFPTLTPEEAAQMLCDAMIDKPKRKASRLGTFGEILYSLSPSTVDIILNT
                     AYNLFPDSTAAKKTGGAGELADGKADDEMSTEAVAMAYLLKGVHF"
     gene            9193..10389
                     /locus_tag="VHF88_06465"
     CDS             9193..10389
                     /locus_tag="VHF88_06465"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEX2161447.1"
                     /translation="MNDEQLTLNGVAERAGVSPATLRRWVKTGLIPQLDGSGSSAWTP
                     AAAAQARVVARLRDRGHTIEQIRQATEDGRLAFGFVEELLPSEDGDASLEDVAEAAGL
                     APALIERLWSSFGLPGQDLDAMSDEDVEAMREAAGVLSAGLPLVASLQLARVYGQALS
                     QIADAEVRLIHLYVHEPLMREGVPGLEMAEQMEVLARELLPRSTSLMEYLHRRYLAHF
                     VAQDVVGHMEVELDGEVDLGRLRVGICFCDLAGYTRFTEEEGDEQALSYVERFVDSVT
                     ETLPDDARVVKTIGDEVMIVGQDIQALTEWAVRFQAEFTARPEPRIGIHYGAALYRDG
                     DYFGREVNLASRVVARARGGEVLATDAVVEHVASGRLAFEGIGQVKLKGFDEPRQLCR
                     ATGLSR"
     gene            11309..11797
                     /gene="tilS"
                     /locus_tag="VHF88_06470"
     CDS             11309..11797
                     /gene="tilS"
                     /locus_tag="VHF88_06470"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX2161448.1"
                     /translation="MSIPRGVPATPEPQGGTRSLDLGNNLRAIAEYGTLRFRAGPEVP
                     TPGPVDLPIPGEARFGGWHLRATAGGGGEVELDAAALGDAVTVRGWRDGDRIRPLGLG
                     GSKSLQDIFTDRKVPRELRRSLPIVEVDGQIAWVAGLAVGERFAVTPATTKRIGLSAR
                     LK"
BASE COUNT         1614 a         4203 c         4308 g         1729 t
ORIGIN      
        1 cggccgcgat ggagcggctg gtcggcgagg gcaagctggg cgccaagacc ggcggcgacg
       61 gcttctactc cgacggccag ccgaacgtcg atggcgacgg cgacgccgac gcggaggagc
      121 tcgccgagct ctgggcgatg aaggcgctgc gcgaggcgct gctggtgctc gaggagggcg
      181 tctccagcgt ccgcgagatc gacctcggcc tgatggccgg cgccgggctc gacccacgcc
      241 gtggcctgct cccgccgttc tggaaggccg acgtcgaggg cctcgacgtg gcgctcgaga
      301 agatggaggc gctcgaggcc aagcacggcc cggagcgctt cggcgtgccg acgatcctgc
      361 ggcggatggt cgcccagggg aggctcggga tcgccgccgg ccagggcttc tacgcctggg
      421 cgcgtccgga cgacggcgat cagcgcgaga cggtcaagct cgagacgcgc ggcgacgtgg
      481 cgatcgcctg gctcgccaac gcgccgatga acgcgatctc cccgcaggtg atcgaggacc
      541 tcggcgccgt ctgggagaag gtgacgagcg agggcgaggt cggcgcgctg gtgatcgcgt
      601 cgtcgatccc ggtcgtcttc tcggccggcg ccgacatcaa ggcgttcacg cagatggacg
      661 aggagaaggg ggcggagctg atccactcgg gccacgccct cctgcgcgac ctcgggtcga
      721 accgcgtctc gaccgtcgcg gcggtcaact cgatcgcctt cggcggcggc tgcgagctcg
      781 cgatggcctg cgacgtgcgc atcgcggcgg agtcggcgat cttcggtcag ccggagatca
      841 agctcgggat catccccggg ttcggcggca cgcagcggct gccgcgactg gtcggggaga
      901 gcaaggcgct cgagatgaac ctgaccggcg atgcgatcac cgccggcgac gcgttcgagc
      961 acggcctcgt gaaccgcgtc gtgcccgacc acgagctgtt cgacacggcg ttgacgtggg
     1021 cgcgcaagct ggcgggccag gcgcccgtcg cggtcgagcg gatcaaggag gtctcggccg
     1081 ccgggaacct cgacgagggg atcgaggccg agaaggccgg cttcgctgcc gtgttcgcga
     1141 ccgaggacgc caaggagggc atcggcgcct tcctcggcaa gcgaacgccc aagtggtcag
     1201 gacgctagag agcaccacag cggagctcgc cggcctcctc cgcggggccg agcgggcgat
     1261 cgtcttgacg ggggcgggga tctcggtgcc atccgggatc cccgacttcc gcagcccggg
     1321 taccgggatc tgggaggggg tcgacccgat ggaggtcgcc cacatcgacg cctggcggcg
     1381 cgacccagac cgcttctgga gcttctacgg ttctcgcttc tcgggtcttg tcgacaagcg
     1441 ccccaaccgc gcccacgagg tcgtcgccga gctcgagcgc agcgggcggg ttcgcgccgt
     1501 gatcacccag aacatcgacc gcctgcaccg tgtcgccggc agtcggcggc tgctcgagat
     1561 ccacggctcg atcgagtcga gcgtctgccc gcaatgcggc ggccggaccg ccgtcgagca
     1621 ggtcgtcgcg ctgctcgccg ccggcgacgg tgcgccggag tgcccggcgt gcatcgcgcc
     1681 gttgaagccc gacgtggtgc tgttcggcga gctgctgccg gccgacgtca tggccgaggc
     1741 cgagcagctc gcgctcgagg ccgacctgat ggtctgcata ggcagctcgc tgaccgtcca
     1801 cccggctgcc gggctgccgc aactcacgct cggctcaggc ggtcggatcg cgatcgtcac
     1861 cgagggcccg acgccgctcg acgaccgtgc ggccgtcaag ctgagcggcg acgtcgtagc
     1921 ggagctcgaa gcggtggccg aagccctgcg aacctagatt gcgctgtcta gccggcgggc
     1981 gcgcagcgcc gactccatcc gcccgacgct cgccgccagc tcgtcggcga gctccgcgga
     2041 cagctcgcca cgcagcgcgg cgttcgccgc gtcgccgacg gcccgccggt cgccctcgag
     2101 ctcaccgcgg cggccgtcgg gcagctgcga cagctcggcc agcagcgcct cgagcgccac
     2161 gcgcgcctgc agggcggcct cacggccgcg tcccgccgcg atgtccattc gcgcgcgcag
     2221 cacgagctcc tcgcaagcga ggacgcgctc gcggccgctg agcagcgccg cgaagcgctc
     2281 ctcgggcgcc tccatcgagc gcttcgcccg cacgatgccg gccgccggca gctccagggc
     2341 ctgcttgaac atccccgcga ccgcctcgtc cccggacccg aagccgatcc gcgtgaccag
     2401 cgcctggctg tggtcgacat ctcgcacatg gggatcggcg cgcgccacgc ggtaggcgtg
     2461 cagcgccccg ttgagcgtcg aaagtgcgcg gtcgagctcg gccgcgcggc ggtcgctgtc
     2521 gtcgcggacg gcggccagcc agaggtcggc gtgatccggc gtggggaagg ggtccgtgtg
     2581 gacgaccgtg gcccggttgg tgggaacggc ctcaggggtg gcctcggtga cggctttggc
     2641 gcggcggccg cggaggcgcc gccgctgcgg ggccgcgagc gtcgagagca cgagcaccgt
     2701 ctcgggccgc tcgcccggat cggcgcggac catgtagcgg ccgtcgcccg gtccgagcag
     2761 aaagccgaac tcgagctgga cgaagccgaa caggggggac gccatcacgc cgaaccgtcg
     2821 cagacgccgt cgggcggcgc gatctgctcc agggcgctac tcgccgtcgt gggcgtgcga
     2881 cgcaggcttg tcggccggca gcgagtactt cgagggctcg gtcggcacga ccgggttgtc
     2941 gatctcgcgt aggcgagcga tcaggtccga ccactggccg ggctcgagca ccgcgagcgc
     3001 ctgctggccg agcctgcggt tctggccgaa caacggctgg aagccgacgt ggatgtggtc
     3061 gtggtggtcg cccatcgcaa ccgtgttctg gccgtagtcg agcaggctga tgatctggtc
     3121 tgggcgcatc gtgccttgca ggcgcatcag tcggcggacg gcctggtcgg tgacgccgcc
     3181 gggctcctgg tggccgaaga tcggcactcc gttgatcgcg gcgacgtcga cggcgttgcc
     3241 cgaggagtgg tgcgagacgt tgccggatgc ggtcaggtag ctgtgacccg acttcaggct
     3301 cgtcacggtc gggcgcagcc cggactcggc gaggtaggcg agcgtcgcga ggaccctgcg
     3361 gtcaacctgg ccggactgga tgtcggagcg gccgccctca tagatctcga tccgctcgtc
     3421 ggccagcacg cgcttctcga gcagcggctt gggcagcagc aggatctggc cgatcgacag
     3481 gcttccgacg cgcgcgtcgc ggccatagag cacgttcttg ccggcggcgc ggtagatcgc
     3541 ggtcgcctcc agcagcttcc agccgtcgag gatcggcttc ggatcgatcg acggggcgcc
     3601 gcggccggcc gggcggatgc cgaagtcgag gtgggcggcc ttcgccgcgt cgaccttgcc
     3661 gagtcggccg aggatcgtcc cgccgatcac acgcgacccg accttcaggc ggcgcaggcg
     3721 gacgtccttg gcgtcggcgc cgaaggggcg cgagaagtag ttgctgtagg tctcgaactt
     3781 gccgtccagg cgggccttgg cggccagcag ctgctccagg ccgccggact cgcgggcgcc
     3841 ctcggcgttc gggtgggcga agaggcgctc cttgaagggc aacgaggcgg tcggctcggc
     3901 ggtggcagtg gctgcacctg cgtcgtcgtc ggtcgcgtcg tcgctggcgg cctggcgacc
     3961 ggctgacgcg gccttggtcg gctccgggtc cacagcgtcg ccgtttgcct tgatcgcccg
     4021 gacgtcggtc gcgtcctcct tcaggacggg gtagtgctcg gcgatctcgc cgagaccggc
     4081 gtaggtgtag cggttgccgt acacgtcctg gagcacgacg tagttgccga ggcgcttcga
     4141 ctcgccgacc ttcttgatca cgccgtcgtt ggtcgcgacg accggcgagc ccttctgggc
     4201 gaagatctcg agcgagcggc ggtcgtcctg cgactcgacg acgttcgcgg cgttctcgcc
     4261 gactctcacc cgcttgaccg cctcctgctc ggcgaggtcg tcggcgtagc gcgcccgcgc
     4321 cgcgaccggg aagcgaccct cggtcaggcc ggtcagcgag ccgacgaggt cggccgggac
     4381 accggagatc aaccgggcgc gcagcagtac cgagtcgacg taccaatcgg cgtggttgta
     4441 ggcgaagatc gcaccgcgga cgtcgttctc gtagttggcc gccttgaggt agcgggcggc
     4501 cgcgaagatc gcgtcgaccg ggttgtacgg gtccttgcgg ccgtccttgt tggcgtcgac
     4561 gccgtaggcc cgccatgacg gcggcatgaa ctgcatccag ccgagagcgc cggcgctcga
     4621 gacgttcagg ttgcggccgt agtcggtctc gatctcgttg atcgccgcga ggatctccca
     4681 gcggatccca tactcgatcc cggcggcctg gtagatcggc agcaggaaga gcgggacgcg
     4741 gaacttgcgg atgacgaagt tcggcacgcc ttgcgctacc gacgggccgg gaagcgcctc
     4801 gaagaaggcg gggttcgagg tcgagggcga gccgtcgggg ttgcgcagcg ccggaggctc
     4861 cgcctgtgcg ccaccccgct catgggcctc accctcaggc gaaggttgag agttcttggt
     4921 cgtgggacgc ttgacggctc gccggtcgtc gccgcccgac ttctgcttcc ggccgccgct
     4981 cggggtaggg gcggggcccg cgggatccgt ctcgagcggc gccggcggtg gggcgggcgc
     5041 agccggcggg gcgggcgtcg tctgcggagc gggagtggtt ggggcgggag gaggcggcgg
     5101 cgtggttgcc gtcggcactg gcggaggcgg cggaggcgtc gtctctgtcg tcgtggcggg
     5161 agcgacctcc tgttgggtca ccgattggac cggtccgata tccgagggca gctggatggc
     5221 cgacaccggc gtgccgggcg gcacgtcgac ggtgatcgtc acgacctcgc cgctgaccag
     5281 cgtgacggtg agcacgcgcg tctcagccga ggccgccacc gtgaagccgg tgagggccgc
     5341 ggtcagcgcg acgcctgcgc aaagcagggt gtgccacagc cgcttcttca tcccggaaat
     5401 tcgtctcctc cttgcacgcc cccggcgggg acgctcttag gcgcgcgcaa gcttatagat
     5461 cggctgggcg caggcagaac gctagctatc gcatcggaga ggtacaaggg ggatcggtaa
     5521 gtcgggaaaa ccccgcgtgg tgcgggcggg cgtccaccga gtatcggcat cgtgcgccat
     5581 ttccgcaaat gctggatgcg tcgttgcggg cgccaggagg cccacccggc cgggcagagc
     5641 cttgtggccg gcttccctaa ccctaagcta cacagtgaga cttcagggta ggcggccctc
     5701 cgctagccga cccttgtagg ctgcgctgcc atggccgccg accgtgaagc tcccttcgtc
     5761 gaccgcgtct cgcagcaggg cgaggaggcg ctcggccgcc ttgccgagca gctgatcggc
     5821 aacccgatcg tcaccggcgc ggtgtcacgc gcctttgagg cgcgcgagaa agccgtccag
     5881 gctcaggagg cggcgctcgg cgcgctgaac atcccctctg ccgccgacat cgagcgcgtc
     5941 acacgacgac tgcgctcggt ctcgcagcgc ctggagtcga tcgaggacac gctcgatcga
     6001 ctcgaggagc gactcgccgc tccaccgccg aagccgaagc cagcgccgaa gacgcgctcc
     6061 aaggccaaga agagctagct cggcgactgc gtcagcgcgg cccagccggc ctcgccgagc
     6121 gcaaggccga gcggctcaac ccggtggtgc gggagccggc ggcgatcgcc gccggcgagc
     6181 aggtctgtga tgccgagcac gacagctgcc gcgacctcgc gccggctcgc gatcgtgagc
     6241 agcgtcgccg cctccatctc gacgacgtcc gctccgctcg ccagccactc ggccgggagg
     6301 tctgaacggg ggtcgtagaa gaggtcggtg ctcacggcgg tgaccggtcg cgcgcctgcg
     6361 ctgactaagg cgtcggtcaa cggtcggtcg ggtgcgagcg gcgattcggc gccgagcgcg
     6421 gcgctcgtcc cgtccgcacc gatcaccgtc gcggccgtca cgagctcacc gagctcgaag
     6481 ccgtcgcgca gcgcgccgca ggtcccgatc cgcacgagcg cgcgggctcc aagctcgatc
     6541 agctcctcga cgacgatcgc cgcgctcggc ccgcccatcc cggtcgcctg tacggtcagc
     6601 ggctccccgt cgcgagctgt gccggtgtag ccccataggc cacggttatg gttgaacatc
     6661 ctcggccgct cgagcagcgc ctgggcgacc gcgagcgcgc ggtggggatc gcctggcagc
     6721 agcacgcgct cggcgagtgt cgtcgcgggc cgcaggtgaa gcgtcgccgg gtcggtcacg
     6781 cgcgccaccc taagggtcgg gtccggcgat cgcggcggcg gcgtggctat cctcgccgtg
     6841 tgcccgaccg gccagacatc caggatgccc tgcgcgacgc agtcgaccgc accgttcgtg
     6901 cgaccgtcga tacccgcgag cgcgcccagg gcgccgcggg cggcgtcgct gacgcagtcg
     6961 atgacctcgt caagggggcg gagcgcaaca tcgcccgcgg ccgtcgcggc gtgctcgccg
     7021 ccgtcgagga ccgtctgccc gccagccaag acgacatgcg ggctctgcgc tccgagctgc
     7081 gccggatcga caggcgcctc gcggagatcg agcgccgtct ctcgaacgtc ggctagaagt
     7141 ggacgccctt gagcaggtag gccatcgcga cggcctcggt cgacatctcg tcgtcggcct
     7201 tgccgtcggc tagctcgccg gcaccgccgg tcttcttcgc cgcggtcgag tccgggaaga
     7261 ggttgtaggc ggtgttgagg atgatgtcga ccgtcgacgg cgagagcgag tagaggatct
     7321 cgccgaaggt gccgagccgc gaggccttgc gcttcggctt gtcgatcatc gcgtcgcaga
     7381 gcatctgcgc cgcctcctcg ggcgtcagcg tcgggaaggc cttgtagatg tcggtgggag
     7441 cgatcatcgg cgtccgcacg agcggcatgt agacggtggt gaacttgacg ttgtcggcga
     7501 cgcactccgg tgcggcgcag cgcgagaagg catccagtgc ggacttcgac gcgacgtaag
     7561 ccgagaagcg cggtgtgttg gtctggacgc cgatcgagga gacgttgatc acatgtcccg
     7621 acttgcgctc gcgcatgccc ggcaggaagg tcatgatcag tttcagcgcg ccgaagtagt
     7681 tgagctgcat cgtccgctcg tagtcgtgga agcgatcgta ggagcgggcg accgagcgtc
     7741 tgatcgagcg gccggcgttg ttgaccagga cgtcgacgcc gccgtgctcg tcgacgatct
     7801 cccgtccgac gcgctcgacg tcgtcgatgt cggagaggtc ggcgggatgg acgtgggccg
     7861 tgccgccggc ggctcggatc tgctcggcga cctcctcgag cttcgcgcgc gtgcgcgcga
     7921 cgaggatcac gatcgcgccg gcctcgccga cccgcaccgc cgtctcgagc ccgatccccg
     7981 acgacgcgcc ggtgatcacg atccgcttgt cgcggactgc cgtcgtcagc gagcggtcgc
     8041 ggaacagcgc cgggtcgagg ttgcgctccc agtagtccca cagccgcccg gcgtagctgt
     8101 cgagcggcgg cacggcgatg ccggtgccgc tcagcgcgcg ttgagcgtcg cgcgcgtcga
     8161 aggtgcactt gaagtcgcgc acctccatcg cctccggcgg gatgccgagg tcgcggtaga
     8221 gctggttgcg gatcttcttg acggtcggca gcgccatcac gccggcgcgt accggcgcgg
     8281 ggacggcctt ggtcaggttc tcgtcgacgc gcatcgcgag ctgtggcgcg tgcgcggccc
     8341 tggcgaacgt gttgaccgcc gcgccgacgg tcatcggctc gggatcgacg aggtggtagg
     8401 tgtcgcccgg gaggtcctcg tcggccatgt gcccgatgtg gtcgatcgcg cgtgccacga
     8461 agtcgaccgg gacgatcgtg gtcttgccgc cctccggacc ggcgagcggc atccaggccg
     8521 gcagcgcgcc gcgcagcttc ttcagcagcg tgaagaggta gtacgggccg tcgatcttgt
     8581 cgatctcgcc cgtctgcgag tggccgacga cgatccccgg ccggtagacg agcgtcttgg
     8641 ccttcacgtc gcggcggacc atctgctcgg actcgtactt cgtccggtgg tagtggtggg
     8701 gcagtcgctg gccctcgtcg agcatgtcct cctggaacac gccgcggtag tcgcccgcga
     8761 ccgcgatcga gctgacgtgg tggaaccgcc ccgggctcag gcggttggcg agctcgatcg
     8821 cgtggcgcgt gccctcgacg ttggcgcgct gcgcggtctg ctcgtcggcc tcgatgtcgt
     8881 agacggcggc gaggtggaag aagtgatcga tcgtgagatc ggctggaagc gcgtcctcgt
     8941 cgacgccgag cgccggctgg gtcaggtcgc ccgggaccgg cacgatccgc ccctcgggcg
     9001 cgttcagccg ttgtgccagc gcgtcgacct tgccgcgcga gccctcccgg acgagcacgt
     9061 agatcgtccc gtcgcgcttc agcagctcct cgacgaggtg cctcccgatg aatcccgtcg
     9121 cgccggttac gaagtagttc acgccgggcg agtgtaaccc ggctcgccac cgctgtagcg
     9181 tcagcggcgt tcgtgaacga cgagcagctc actctcaacg gggtcgcgga gcgagcgggc
     9241 gtctcgcccg cgacgttgcg acgctgggtc aagaccggcc tgatccccca gctcgacggg
     9301 agcggcagca gcgcctggac cccggcggcc gcggcccagg cgcgcgtagt ggctcggctc
     9361 cgcgatcgcg gccacacgat cgagcagatc cgccaggcga ccgaggacgg acggctcgca
     9421 ttcggcttcg tcgaggagct gctgccgagc gaggacggcg acgcgtcgtt ggaggacgtc
     9481 gccgaggccg ccggtctggc cccggcgctg atcgagcggc tgtggagcag cttcggcctc
     9541 cccggccagg acctcgacgc gatgtccgac gaggacgtcg aggcgatgcg cgaggccgcg
     9601 ggcgtcctct cggccgggct gccgctggtg gcctcgctgc agctcgcgcg cgtctacggc
     9661 caggcgctgt ctcagatcgc cgacgcggag gtgcgcctga tccacctcta cgtccacgag
     9721 ccgctgatgc gcgagggcgt ccccggtctc gagatggcgg agcagatgga ggttctcgcg
     9781 cgcgagctgc tgccgcgctc gacgagcctg atggagtacc tgcaccggcg ctacctggcg
     9841 cacttcgtcg cgcaggacgt cgtcggccac atggaggtcg agctcgacgg cgaggtcgat
     9901 ctcggccggc tgcgcgtcgg catctgcttc tgcgacctgg ccggctacac gcgcttcacc
     9961 gaggaggagg gcgacgagca ggcgctctcc tacgtcgagc ggttcgtcga cagcgtcacc
    10021 gagacgctgc ccgacgacgc gcgcgtcgtc aagacgatcg gcgacgaggt gatgatcgtc
    10081 ggccaggaca tccaggcgct gaccgagtgg gcggtgcgct tccaggccga gttcaccgca
    10141 cgccccgagc cgcgcatcgg catccactac ggcgccgcgc tctaccgcga cggcgactac
    10201 ttcggccgcg aggtcaacct cgcttcgcgc gtcgtcgcgc gcgcccgcgg cggcgaggtg
    10261 ctggcgaccg acgcggtcgt cgagcacgtc gcctccggtc gtctggcctt cgaggggatc
    10321 ggccaggtca agctcaaggg cttcgacgag ccgcgccagc tctgccgggc gaccggcctg
    10381 tcgcgttgac cgctgcggcc gaccccggtg ccgcgctcgc cgccgcccgc gccagcgggc
    10441 tcgtgcgccg gggcgagccg ctgctcgtga tgctctccgg cggcgccgac tccgtctgcc
    10501 tgctcgacgt cgctctgcgc ctcggcgccg acgtcagcgc cttgcacgtc aactacggcc
    10561 tgcgcgaggc cgccgctggc gacgaacacc actgccgcga cctctgcgcc gcgctcggcg
    10621 tcgacctgac cgtcgaggcc gtctcgctgc cgcgcgaggg aaacctccac gccctcgcgc
    10681 gcgacgcccg ctaccgcctc gccgagcgcc acgccggcgg cgactacgcc gccgcccaca
    10741 cgctctccga ccaggccgag accgtgctct atcgcctcgc cgcctcgccc ggccgccgcg
    10801 cgctgctcgg tatggagccg cgccgcggca ggctcgtccg gccgttgctc ggcgcgaccg
    10861 ccgaggagac ccgcgcctac tgcgaagccc acggactcgc ctggcgcgac gacgcctcca
    10921 acgacgaccc ccgcttcgcc cgcgcgcgcg tccgccacgg cctcctgccc gccctgcgcg
    10981 agatcgctcc cgccgccgag cgcacgatcg ccgagaccgc cgcccagctc cgcgacgagg
    11041 ccgacgtcct cgacgccgcc atcaccgacg ccctccaacg cctcggcggc ggccccgccg
    11101 tcgaggctgc cgccctcgcc gagctcggcc ccgccctcgc ccgcctcgtc ctccgccgcc
    11161 tcgccgagcg cgaggccggc gcgccccacc cgctcacccg ctccgacgtc gaggccatcc
    11221 tcgccctcgc aggcgcaacg ccatcccaca gcccggccga catagccccg gggaatgaca
    11281 tccaagcagc cgttcccaag cggattggat gtccattccc cggggcgtac cagccacgcc
    11341 cgagccccag gggggaaccc gttccctcga cctcggcaac aaccttcgcg ccatcgccga
    11401 gtacggcacg ctccgcttcc gcgccggccc cgaggttccc acccccgggc ccgtcgacct
    11461 gcccatccca ggcgaagccc gcttcggcgg ctggcacctc cgcgccaccg caggaggcgg
    11521 tggcgaggtc gagctcgacg ccgccgccct cggcgacgcc gtcacggtcc ggggctggcg
    11581 cgacggcgac cgcatccgtc cgctcggcct gggcggcagc aagtccctcc aggacatctt
    11641 caccgaccgc aaggtccccc gcgagctccg ccgctcgctc cccatcgtcg aggtcgacgg
    11701 ccagatcgcc tgggtcgccg gcctcgccgt cggagagcgc ttcgccgtca cgccagccac
    11761 gaccaagcgc atcggactgt ccgcgcggct caagtagccg tcccgacggg catccccgag
    11821 cagcgattcc caagcggatt ggatgtccat tccc
//
LOCUS       DATABV010000147         5496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1169466, whole genome shotgun sequence.
ACCESSION   DATABV010000147 DATABV010000000
VERSION     DATABV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169466"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="VHF88_06475"
     CDS             <1..1621
                     /locus_tag="VHF88_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX2161449.1"
                     /translation="VKEFLSRGTYAFPPEPSLRLIADMIAFTVDEVPKWNPINICSYH
                     LQEAGATPVQEIAYALSTAIAVLDEARPRVPEETFPRVFGRISFFVNAGIRFIEEHAK
                     LRAMGRLWEEIGRERYGVTEQKFLRMRYGVQVNSLGLTEAQPENNVIRIVLEALAVTL
                     GRDARARALQLPAWNEALGLPRPWDQQWSLRIQQILAHETDILDYPDIFEGSKVMDGL
                     VEELAEGARAEMAVVAEHGGAVAAVPYMKTQLVESHRARVGAIESGEQKVIGQNSFTS
                     TEPSPLQQGEDGGILTVDPAVEQNQIAAIERWRGERDGAAVKEALDALRQAAADPGAN
                     IMPPTLAAAKAGVTTGEWATALREVFGEYRAPTGVADAAAAPGDEALDELRDRVERVS
                     EELGRRIKILVGKPGLDGHSNGAEQIAVRARDAGMDVVYEGIRLTPSRIAQAAVQEGV
                     HVVGLSILSGSHRELIPQVLDELHAAGADVPVVVGGIIPRADEEELLAAGVARVYTPK
                     DFEVSRIMGDIVDVVAERHGAAAVLAGPRST"
     gene            1694..2665
                     /locus_tag="VHF88_06480"
     CDS             1694..2665
                     /locus_tag="VHF88_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2161450.1"
                     /translation="MTVDGRQLGARLRDGDRSAAPAALNLIEDRSTHGREGAAALLRE
                     VSPAALGSEAPAHLIGLTGPPGAGKSTLLSALVHEWRKRGQTVAVLAVDPSSRRSGGS
                     LLGDRARIEHDPGDDGILIRSTAAGGRLGGLAAPTREAAQALAAAFDVVVIETVGVGQ
                     SETDVEEVCDTVAVVVQPGSGDTLQFLKAGIMEVPDVLVVTKADLGDVALRSRRDLSQ
                     AARALGSRDIPVIAVSSVPPPSGIAELVDALAAHRDRIDLQARRLRSRRLTALRELVA
                     EHGESALRALGGRREAERLLSDLDPGLDVLELVATLRERSNREDAAR"
     gene            2662..2817
                     /locus_tag="VHF88_06485"
     CDS             2662..2817
                     /locus_tag="VHF88_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161451.1"
                     /translation="MTLPVQAALLLGVLAATTALSLLAGAANLGTALGIGQIAFTIVL
                     VWLLLRR"
     gene            2843..3448
                     /locus_tag="VHF88_06490"
     CDS             2843..3448
                     /locus_tag="VHF88_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161452.1"
                     /translation="MEGSAPDSVGTRSSWLRCYRCWSQNLEVQVHYEGILKVDTDTAE
                     AVEPVEEVQEAVVQCLDCMHDQPHLSLAFYEEDGRRVSRIEAVDDRWERMVTGTPWVA
                     SCTVTVEGDQVESCSGPEAADSLAFAAFGDHGTREFFTHVRFHKHEDDGQVVVHMLVE
                     LYARSAEEATDVLEGAARGALAITSLAEESRPPAAAEDEGH"
     gene            3474..4076
                     /locus_tag="VHF88_06495"
     CDS             3474..4076
                     /locus_tag="VHF88_06495"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX2161453.1"
                     /translation="MATRGRSWPRAGLRFLASVMIVSGTLLVADAVLTLAWQEPISAI
                     VAQRNQSDLEQALANPPRRVVEGRPLPGDAVAKLEIPAIGVSEYVVEGTDTGDLRKGP
                     GHYPETPLPGERGTSAIAGHRTTYGAPFRRLDDLDPGDRIVVELPRERHTYRVERLRI
                     VDDSDRSVLRRVGHDRLVLTACHPLYSAEQRIVVFARADS"
     gene            complement(4083..4226)
                     /locus_tag="VHF88_06500"
     CDS             complement(4083..4226)
                     /locus_tag="VHF88_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161454.1"
                     /translation="MLLRLVYWLVVVAISLALVVGLILYLESRDQSSVEGANAAPAAV
                     ESV"
     gene            complement(4237..4731)
                     /locus_tag="VHF88_06505"
     CDS             complement(4237..4731)
                     /locus_tag="VHF88_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2161455.1"
                     /translation="MRDEEFERLYEDHAAGLLAFLMYRTGDRTLAEDVVADTFERVLL
                     ARRRFDPRRGSRKTWVYSIALNRLRDLQRSRQSEQRALTRIEPQASGGAGDFEAIEHQ
                     DELTAALAQLSPEEREAIALRYGGDLTVPEIARLQGERLSTVEGRVYRGLRKLRELLD
                     RPGS"
     gene            4786..5439
                     /locus_tag="VHF88_06510"
     CDS             4786..5439
                     /locus_tag="VHF88_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161456.1"
                     /translation="MDQSNNPAPGDLSEVADRLRESRSVPTPLELDELKLRARHQAAR
                     SSTSPGGNMLKSRLALTALIAVGAMMSGTGATLAVTGSSGSGSAEEHQYTTPTTTTET
                     TDTTPTTETETTPTTETVTTTTTVTETTSTSQVDETRTNQSNDTPQPTLAPQDVAGDQ
                     DEGGDDGDSSPSEQVQVAGDSDDSLPFTGFLAIPLLIGGVAMIATGGILRHRSRDSQ"
BASE COUNT          821 a         2003 c         1945 g          727 t
ORIGIN      
        1 cgtcaaggag ttcctctcgc gcgggaccta cgcgttcccg cccgagccgt cgctgcggtt
       61 gattgccgac atgatcgcct tcaccgtcga cgaggtcccg aagtggaacc cgatcaacat
      121 ctgcagctac cacctgcagg aggcgggcgc gacgccggtc caggagatcg cctacgcgct
      181 ttcgacggcg atcgcggtgc tcgacgaggc gcgcccgcgt gtgcccgagg agaccttccc
      241 gcgcgtcttc gggcggatct ccttctttgt caacgccggc atccgcttca tcgaggagca
      301 cgcgaagctg cgcgcgatgg gccggctgtg ggaggagatc ggccgcgagc gctacggcgt
      361 gaccgagcag aagttcctgc gcatgcgcta cggcgtgcag gtcaactcgc tcgggctgac
      421 cgaggcacag ccggagaaca acgtgatccg gatcgtgctc gaggcgctcg cggtgacgct
      481 cggccgcgac gcccgcgccc gcgcgctgca gctgccggcg tggaacgagg cgctcggcct
      541 gccgcggcca tgggaccagc agtggtcgct gcggatccag cagatcctcg cccacgagac
      601 cgacatcctc gactacccgg acatcttcga ggggtcgaag gtgatggacg gcctcgtgga
      661 ggagctggcc gagggcgccc gggcggagat ggcggtcgtc gccgagcacg gtggcgccgt
      721 cgcggcggtg ccctacatga agacccagct cgtcgagtcc caccgcgccc gcgtcggggc
      781 gatcgagagc ggtgagcaga aggtgatcgg ccagaactcg ttcaccagca ccgagccgtc
      841 gccactccag cagggcgagg acggcggcat cctgaccgtc gatccggcgg tcgagcagaa
      901 ccagatcgct gcgatcgagc gctggcgcgg cgagcgtgat ggagcggcgg tgaaggaggc
      961 gctcgacgcg ctccgccagg cggcggccga cccgggcgcg aacatcatgc cccccacgct
     1021 cgcggccgcc aaggccggcg tgacaaccgg cgagtgggcc accgccctgc gcgaggtctt
     1081 cggcgagtac cgcgccccga ccggcgtcgc cgacgccgcc gcggcgcccg gcgacgaggc
     1141 gctcgacgag ctgcgcgacc gcgtcgagcg ggtctccgag gagctcggcc ggcggatcaa
     1201 gatcctcgtc ggcaagccgg gcctcgacgg ccactccaac ggcgccgagc agatcgccgt
     1261 gcgggcgcgc gacgccggca tggacgtcgt ctacgagggc atccgcctga cgccgtcgcg
     1321 gatcgcccag gccgccgtcc aggagggcgt ccatgtcgtc ggcctctcga tcctctcggg
     1381 ctcgcaccgc gagctgatcc cccaggtcct cgacgagctg cacgccgccg gcgccgacgt
     1441 cccggtcgtc gtcggcggga tcatcccgag ggccgacgag gaggagctgc tggcggccgg
     1501 cgtcgcccgg gtctacacgc cgaaggactt cgaggtgtcg cgcatcatgg gcgacatcgt
     1561 cgacgtcgtc gccgagcgac atggtgccgc cgcggtgctc gccggacctc gctccaccta
     1621 gcgtcggcat gtagcggcgg gaccgttccg gctgcgctct ccgctcagga cggtctcggc
     1681 tagcgtgccg cccgtgacgg tcgacggacg gcaactgggc gcacggctgc gcgacggcga
     1741 ccggtcggcc gctccggcgg cgctgaacct gatcgaggac cgctcgacgc acggacgcga
     1801 aggcgccgcg gcgctgctgc gagaggtctc ccccgcggcg ctcggcagcg aggcgccggc
     1861 gcacctgatc gggctgactg gcccaccagg ggccggtaag tcgacgctgc tgagcgcgct
     1921 ggtccacgag tggcgcaagc gcggccagac ggtcgcggtg ctcgcggtag atccctcctc
     1981 gcgccgctcg ggagggtcgc tgctcggcga ccgcgctcga atcgagcacg accctggcga
     2041 cgacggcatc ctgatccgct cgaccgcggc cggtgggcgg ctcggtggcc tggccgcccc
     2101 gacccgcgag gccgcgcagg cactcgccgc cgcctttgac gtggtcgtga tcgagaccgt
     2161 cggcgtgggc cagtccgaga ccgacgtcga ggaggtgtgc gacacggtcg cggtcgtcgt
     2221 ccagcccggc tcgggcgaca cgctgcagtt cctcaaggcc gggatcatgg aggtgccaga
     2281 cgtgctggtc gtgaccaagg ccgacctcgg cgacgtcgcg ctgcggtcgc ggcgcgacct
     2341 cagccaggcc gcccgggcgc tcggctcgcg cgacatcccg gtgatcgccg tctcctccgt
     2401 cccccctccc tccggcatcg ccgagctcgt ggacgccctg gccgcccacc gcgaccgaat
     2461 cgacctccag gcccgccggc tgcgctcgcg ccgcctgacc gccctgcgcg agctcgtggc
     2521 cgagcatggc gagtcggccc tccgcgccct cggcggccgg cgcgaggccg agcgcctgct
     2581 gagtgacctc gaccccggcc tcgacgtgct cgagctggtc gccacgctgc gcgagcggtc
     2641 gaaccgcgag gatgccgcgc gatgaccctt cccgtccagg ccgccctcct actcggcgtc
     2701 ctcgccgcca cgacggcgct ctcactgctc gccggcgccg caaacctcgg caccgcgctc
     2761 ggaatcggcc agatcgcctt cacgatcgtc ctggtctggc tgctgctcag gcgctagctc
     2821 cgccgccggt cataatggcc gcatggaggg ctccgctcca gattccgtcg gaacgcgctc
     2881 cagctggctg cgctgctacc gctgctggtc gcagaacctc gaggtgcagg tgcactacga
     2941 ggggatcctc aaggtcgaca ccgacaccgc cgaagctgtc gagccggtcg aggaggtgca
     3001 ggaggcggtc gtccagtgcc tcgactgcat gcacgatcag ccgcacctga gcctggcgtt
     3061 ctacgaggag gacggccgcc gggtcagccg catcgaagcg gtcgacgacc gctgggagcg
     3121 gatggtcacg ggcacgccgt gggtcgcgtc ctgcacggtc accgtcgagg gcgaccaggt
     3181 cgagagctgc tccggccccg aggccgccga ctcgctcgcc ttcgcggcct tcggcgacca
     3241 tggcacgcgg gagttcttca cccacgtccg cttccacaag cacgaggacg acggccaggt
     3301 cgtggtccac atgctggtcg agctctacgc ccgctcggcc gaggaggcga ccgacgtgct
     3361 cgagggcgcc gcgcgcggcg cgctggcgat cacgtcgctg gccgaggagt cgcggccgcc
     3421 cgccgccgcg gaggacgagg ggcactagcc ccagcgcgac ctacactggc tcgatggcca
     3481 cgagagggcg cagctggccg cgagccggcc tccgcttcct ggcgtcggtg atgatcgtca
     3541 gcgggaccct gttggtcgcc gacgccgtac tgacgctcgc atggcaggag ccgatctcag
     3601 cgatcgtcgc gcaacgcaac cagtccgacc tcgagcaggc gctcgcgaac ccgccgcggc
     3661 gcgtcgtcga gggccggccg ctgcccggag acgcggtcgc gaagctcgag ataccggcga
     3721 tcggcgtctc ggagtacgtc gtcgagggca ccgacacggg cgatctgcgc aagggaccgg
     3781 gccactaccc ggagaccccg cttcccggcg agcgcggcac ctccgcgatc gccgggcaca
     3841 ggacgaccta cggcgcgccg ttccggcggc tagacgacct cgaccccggc gaccggatcg
     3901 tggtcgagct gccgcgcgag cgccacacct accgcgtcga gcggcttcgg atcgtcgacg
     3961 actccgatcg ctcggtcctg cgcagggtcg gccacgaccg gctggtcctg accgcctgtc
     4021 acccgctcta cagcgcggag cagcgcatcg tcgtcttcgc gcgcgccgac tcctagggtg
     4081 cgtcagacgg actcgacggc cgccggcgcc gcgttcgcgc cctcgaccga ggactggtcg
     4141 cgcgactcga ggtagaggat cagcccgacc accagggcga gcgagatcgc gaccacgacc
     4201 agccaataga cgagccggag cagcaccgcg gcgattctac gagccaggtc gatcgagcag
     4261 ctcccgcagc ttgcgcagcc cgcggtagac gcgaccctcg acggtgctca gccgctcgcc
     4321 ctggagtcgc gcgatctcgg gcaccgtgag atcgccgccg tagcgcaggg cgatcgcctc
     4381 gcgctcctcc gggctcagct gcgcgagcgc ggccgtcagc tcgtcctggt gctcgatcgc
     4441 ctcgaagtcg ccggctcctc ccgaggcctg gggctcgatc cgcgtcagcg cgcgctgctc
     4501 ggactgacgg ctgcgctgga ggtcgcgcag ccggttgagg gcgatcgagt agacccaggt
     4561 cttgcgactg ccgcgacgcg ggtcgaagcg ccggcgggct agcagcacgc gctcgaaggt
     4621 gtctgcgacg acgtcctcgg cgagcgtccg gtcgcccgtg cggtacatga ggaaggccag
     4681 cagccctgcg gcgtggtcct cgtagaggcg ctcgaactcc tcgtctcgca tcccggcatc
     4741 ttgtccgata aggaaaccgg gccgagctcc gtataagggg ttgtgatgga ccaaagcaac
     4801 aaccccgccc ctggcgacct ctcggaggtg gccgatcggc tgcgcgagag tcggtcggtt
     4861 ccgacgccgc tcgagctcga cgagctcaag ctgcgcgcga ggcaccaggc ggcacgaagc
     4921 agcacttccc caggaggaaa catgttgaag tcgcgactcg cactcaccgc gctgatagcc
     4981 gtcggcgcga tgatgagcgg cacgggcgcc acgttggcgg tcaccggcag ctccggctcc
     5041 ggtagcgccg aggagcacca gtacacgacg cccaccacca ccacggagac gacggacacg
     5101 actccgacca cggagacgga gacgactccg acgacggaga cggtcacgac cacgaccacg
     5161 gtcaccgaga cgacctcgac gagccaggtc gacgagaccc ggacgaacca gtcgaacgac
     5221 acgccgcagc cgacgctcgc gccacaggac gtggcgggtg accaggacga gggcggcgac
     5281 gacggcgact cctctccgtc ggagcaggta caggtcgccg gggacagcga cgactcgctg
     5341 ccgttcaccg gcttcctcgc catcccgctg ctgatcggcg gcgtggcgat gatcgcgacg
     5401 ggcggaatcc tgcgccacag gtcgcgcgac tcccagtaga gccgcggacc tctagccgct
     5461 agaggatcgg gaggcgggcc tcgatggccc gccaac
//
LOCUS       DATABV010000148         4282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2536212, whole genome shotgun sequence.
ACCESSION   DATABV010000148 DATABV010000000
VERSION     DATABV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536212"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1357
                     /locus_tag="VHF88_06515"
     CDS             119..1357
                     /locus_tag="VHF88_06515"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX2161457.1"
                     /translation="MADPGLAAAGAARVAWADGQMPVLRLIRERFAAERPLAGVRVAA
                     CLHVTAETACLARTLVAGGAEVGLCSANPLSTQDDVAAALARDDSIDVHAQHGEDLDT
                     YLSHVTALVESDPQITLDDGADLLTAIHAREPDEGLIGGTEETTTGLLRLRRLEADGA
                     LRCPVLAVNEALAERTLNDRYGTGQSALDGILRAANLLLAGHTLVLLGYGFAGRGSAQ
                     RARGAGAAVIVCEVDPLRALEARMEGYEVMPALEAAGRGDLFVTVTGSRSVLRREHFE
                     RMKDNAVLANAGHFDVEIDLDDLRSLATGGIQPVLPLVDQYDLGDRRLNLLASGRVVN
                     LAAGEGHPAAVMDVSFALQALAAEHLALHRDGLDSRVLDVPRAIDDEVARLKLAALDV
                     QIDSLTAEQHAYLEGWRPVQ"
     gene            1415..2551
                     /locus_tag="VHF88_06520"
     CDS             1415..2551
                     /locus_tag="VHF88_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX2161458.1"
                     /translation="MDAAVFDEGSPGRLATTPDGHPAFVPGPAPRELPLSSAAVRSLD
                     EAATNLGILNGLGRRLPNPHLLISPYLRREAVLSSRIEGTQTTMSDLFASELNQLELV
                     RAPDVREVQNYVTAYEVGLASEVPLSLRLIRTLHRELMTGVRGNERQPGEFRQYQNFI
                     GGYSAAEATYVGPPPDEMARCLDDLERFTHEDALPPLVQAAVIHYLFEAIHPFGDGNG
                     RVGRLLIALFLRDRGLLPRPLLYLSAYFERTRSTYYELLLRVSTGGEWDLWVRYFLDG
                     VSIQAQEAADLADRLLEMQGEYRERLLSARATATALALIDHLFHNPLVSTRRVQLALD
                     VTPPTARGAIKTLEEHGIVREITGRRRGRIYRADEILDLLRGDQ"
     gene            complement(2581..2823)
                     /locus_tag="VHF88_06525"
     CDS             complement(2581..2823)
                     /locus_tag="VHF88_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161459.1"
                     /translation="MVGHSVVELPTRRVGTTRVHGRRAAIMKAPPYPKGGIHGDHAIV
                     MWNEGGHGYLVSAHSESSRRATQAAIGIARSSRAEN"
     gene            complement(2947..3798)
                     /locus_tag="VHF88_06530"
     CDS             complement(2947..3798)
                     /locus_tag="VHF88_06530"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX2161460.1"
                     /translation="MFGDNVVFVGATDPQLHWLPDPGVLAPIALLVFVYVRRFRSARR
                     EEGGRGAGWPQALAFAAGVLALLIAVASPVDGLGEDYLFSAHMVQHVLLGDIAPALLL
                     LSLSRVIMRPATRRLHKIERALGPLASPWTGIALWLLLMYVWHIPALYDAALENPFVH
                     LFEHVSFFTAGVCVWWPLIQPVPMRRPLKGFQTVAYIGTAKFGLAALGLYLTWSSSVF
                     YPYYETVPRIWGLSAINDQNAGGAIMMVEQSLTFVIALAFLFSRMLTQSEVDERRRER
                     LEERAAG"
     gene            3858..>4282
                     /locus_tag="VHF88_06535"
     CDS             3858..>4282
                     /locus_tag="VHF88_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161461.1"
                     /translation="MGPLAPILGLIAPPSCWACGARARQREPLCLACRARLRWLDYEP
                     VDLAGVKVWAPVAYDGPARALVAGLKFRGAVGLASPMAAQIAAGAPTRLLRPPALLVP
                     VPLQPSRARARGYNQAERLAAELGARTGMRVSDCLRRARR"
BASE COUNT          647 a         1496 c         1494 g          645 t
ORIGIN      
        1 cgcctccacc tcgacgcgct cggcgtgaag ctgaccgagc tgtccgagga gcaggccgcc
       61 tacatcggcg tcccggtcga gggcccgtac aagcccgacc attaccgcta ctagccacat
      121 cgccgacccg ggtctcgccg ccgccggcgc ggcccgggtc gcgtgggcgg acgggcagat
      181 gcccgtcctg cggctgatcc gcgagcggtt cgccgcagag cggccgctgg cgggcgtgcg
      241 ggtggcggcg tgcctgcacg tcaccgccga gacagcctgc ctcgccagga cgctggtcgc
      301 cggcggcgcg gaggtcggcc tctgctcggc gaacccgctc tcgactcagg acgacgtcgc
      361 cgccgcgctc gcgcgcgacg actcgatcga cgtccatgcc cagcacggcg aggacctcga
      421 cacctacctc tctcacgtca ccgccctggt cgagtcagac ccccagataa cgctcgacga
      481 cggcgccgac ctgctgaccg cgatccacgc ccgtgagccc gacgagggcc tgatcggggg
      541 gacggaggag acgacgacgg gcctgctgag gctgcgccgg ctcgaggcgg acggcgcgct
      601 gcgctgcccg gtgctcgcgg tcaacgaggc gctcgccgag cggacgctga acgaccgcta
      661 cgggaccggc caatccgcgc tcgacggcat cctgcgagcc gccaacctgc tgctcgccgg
      721 gcacacgctg gtgctgctcg gctacggctt cgcggggcgg ggcagcgccc agcgggcccg
      781 cggcgccggc gccgcggtga tcgtctgcga ggtcgacccg ctcagggcgc tcgaggcgcg
      841 catggagggc tacgaggtga tgccagcgct cgaggctgcg gggcgcggtg acctcttcgt
      901 gacggtcacg ggctcccgct cggtcctgcg ccgcgagcac ttcgagcgga tgaaggacaa
      961 cgccgtgctc gccaacgccg gccacttcga cgtcgagatc gacctcgacg accttcgctc
     1021 gctcgcgacg gggggcatcc agccggtcct gccgctggtt gaccagtacg acctcggcga
     1081 ccgccgtctc aacctgctcg cctccgggcg ggtggtcaac ctcgccgccg gcgagggcca
     1141 cccggccgcc gtgatggacg tctcgttcgc gctccaggca ctcgcggccg agcacctcgc
     1201 cctccaccgc gacggcctcg actcgcgcgt gctcgacgtc ccgcgcgcga tcgacgacga
     1261 ggtcgcccgc ctcaagctcg ccgccctcga cgtccagatc gacagcctga ctgccgagca
     1321 gcacgcctac ctggagggct ggcgcccggt gcaatgaaag agcagagact cttcgcatgc
     1381 agctatgaaa gagattgccg ttagtctttc ttagatggat gctgccgtct ttgacgaagg
     1441 ctctccgggc cgtctagcca cgacccccga tggccacccc gcttttgtac cgggacctgc
     1501 accgcgtgag ttgccgctct caagtgccgc cgtgcgctcg ctcgacgagg cagcaaccaa
     1561 tctcggcatc ttgaatgggc tcggtcgccg gctcccaaat cctcatctgc tgatctcgcc
     1621 ttacctgcgt agggaggcag tgctcagttc gcggatcgag ggaactcaga cgaccatgtc
     1681 cgacctgttc gcgtcggagc tgaaccaact cgagcttgtg cgcgctcccg acgttcgcga
     1741 ggtccagaac tacgtgacgg cgtatgaggt aggcctcgca agtgaggtgc ctttgagctt
     1801 gcggctcatt cggactctcc accgtgagtt gatgaccggg gtgcggggca acgagcgaca
     1861 gccgggagag ttccgccagt accagaactt cattggcggc tatagcgctg ccgaggctac
     1921 gtacgtaggc cctccgcctg acgagatggc gcgctgcctc gatgatctgg agcgcttcac
     1981 tcacgaggac gcgctacctc ccctggtcca agccgctgtc atccactacc tcttcgaggc
     2041 gatccatccg ttcggcgatg gaaatggtcg cgtgggacgt ttgctgatcg cgctgtttct
     2101 gcgcgaccgt gggttgctgc cccgacctct tctgtatctg tcggcctact tcgagcgcac
     2161 gcgctccact tactacgagc tgttgctacg ggtgagcacg ggaggtgagt gggatctgtg
     2221 ggtgaggtac ttcctcgacg gcgtcagcat ccaggcgcag gaggccgccg atctggcgga
     2281 tcggctcttg gagatgcagg gcgagtatcg cgagcggctg ctgtcggcga gagctacggc
     2341 tactgcgctg gcgttgatcg accatctctt ccacaatccc ttggtctcga ccagacgtgt
     2401 gcagctcgcg ctcgacgtga ccccgccaac cgcccgtggg gcgatcaaga cgttggagga
     2461 gcacggcatc gtgcgcgaga taacgggaag acgtcggggg cggatctatc gagctgacga
     2521 gatcctggac ctgctgcgtg gcgaccagtg aagctcgcct cgcgacggac ttgcgcctac
     2581 tcagttctcc gctcgtgagg agcgcgcgat tccgatcgcc gcctgggtgg ctcgacgcga
     2641 gctctcgctg tgcgccgaga cgaggtagcc gtgtccgccc tcgttccaca tgacgatcgc
     2701 atggtcaccg tggatcccgc ccttcggata gggcggcgcc ttcatgatcg ccgcgcggcg
     2761 accgtgcacc cgggtggtgc cgacacgacg ggtcggcagc tcgacgacgc tgtggccgac
     2821 cacgcgaccc ctgcgcgggc ccgtgcagcg cgggctgctt cgacggtccg tcgccgcagc
     2881 ccgcgaggaa caggagcgcc agcgtcgcag ggccaactcg aaggcgcatc gttggctacg
     2941 cccggctcag ccggcggcgc gttcctccag tcgctcgcgg cgccgctcgt cgacctccga
     3001 ctgggtcagc atccgcgaga agaggaaggc aagggcgatg acgaaggtca gcgactgctc
     3061 gaccatcatg atcgcgccgc cggcgttctg gtcgttgatc gcgctcaggc cccagatccg
     3121 gggaaccgtc tcgtagtagg gatagaagac ggacgaggac caggtcaggt agagcccgag
     3181 cgcagcgaga ccgaacttcg ccgtcccgat gtaggcgacg gtctggaagc ccttcagcgg
     3241 acggcgcatc ggcaccggct ggatcagcgg ccaccagacg cagaccccgg cggtgaagaa
     3301 ggagacgtgc tcgaagaggt ggacgaacgg gttctcgagc gcggcgtcgt agagcgcggg
     3361 gatgtgccag acgtacatca gcagcagcca gagcgcgatc ccggtccagg gcgaggcgag
     3421 cggcccgagc gcgcgctcga tcttgtgcag gcggcgggtc gccggccgca tgatcacccg
     3481 cgacagcgac agcagcagca gcgcgggcgc gatgtcgccg agcagcacgt gctggaccat
     3541 gtgcgccgag aagaggtagt cctcgccgag cccgtcgacg ggcgaggcga cggcgatcag
     3601 cagcgcgagg acaccggcgg cgaacgccag cgcctgcggc cagccggcgc cgcggccgcc
     3661 ctcctcgcgc cgggcgctgc gaaagcggcg cacgtagacg aagaccagca gcgcgatcgg
     3721 ggcgagcacg ccgggatcgg gcagccagtg cagctgcggg tcggtcgcgc cgacgaacac
     3781 gacgttgtcg ccgaacacgc gtctgattgt gtcaccgcgg tcgtcacggg ccgtcgcaac
     3841 gccgcgacga tcgaccgatg ggaccgctcg cgccgatcct cggcctgatc gcgccgccgt
     3901 cgtgctgggc gtgcggcgcc cgagcccggc agcgcgagcc gctgtgcctc gcctgccgcg
     3961 cgcggctgcg ctggctggac tacgagccgg tcgacctcgc cggcgtgaag gtctgggccc
     4021 ccgtcgccta cgacggaccg gcgcgggcgc tggtggctgg tctgaagttc cgtggcgccg
     4081 tcggcctggc gtcgccgatg gcggctcaaa tcgcggccgg cgccccgacg cggttgctgc
     4141 gcccgccagc gctgctcgtg ccagttccgc tgcagccgag ccgcgcccgc gcccgcggct
     4201 acaaccaggc cgagcggcta gccgccgagc tcggggcgcg gaccgggatg cgcgtctcgg
     4261 actgtctgcg ccgcgcgcga cg
//
LOCUS       DATABV010000149         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1090870, whole genome shotgun sequence.
ACCESSION   DATABV010000149 DATABV010000000
VERSION     DATABV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090870"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VHF88_06540"
     CDS             <1..287
                     /locus_tag="VHF88_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2161462.1"
                     /translation="ELGDFVEDEAALSPFDTASENIRRENVRTVLEALPPREREVLEM
                     RYGLKGHQARTLEEVGRAFGVTRERIRQIENNTLKKLENLPEAQRLREAS"
     gene            370..451
                     /locus_tag="VHF88_06545"
     tRNA            370..451
                     /locus_tag="VHF88_06545"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Tyr,seq:gta)
     gene            453..932
                     /locus_tag="VHF88_06550"
     CDS             453..932
                     /locus_tag="VHF88_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2161463.1"
                     /translation="MPNLNDPQYDEQRDKEGFRARRARIGYQLGTERLGISQWELSPG
                     EAAYPYHLHLAEEELLIVLKGTLALRTPEGWRDLAEGEVVSFPRGEEGAHQVVNRTDS
                     TVSFLAISTNGEPDIVLYPDSNKVGASERRPQGGGLRTFFRLDDVVDYYEGEQPPTD"
     gene            963..1035
                     /locus_tag="VHF88_06555"
     tRNA            963..1035
                     /locus_tag="VHF88_06555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:996..998,aa:Thr,seq:ggt)
     gene            complement(1062..1463)
                     /locus_tag="VHF88_06560"
     CDS             complement(1062..1463)
                     /locus_tag="VHF88_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2161464.1"
                     /translation="MSAETVVYLDSSALVKLIVAESESEALAAYLGDHPTRASSALAR
                     VEVLRATRPHGPRAMARARQLLARISLLHLDDALLDAAALLEADRLRSLDAIHIASAQ
                     TVTSNLAALVTYDHRMAQAAEDLGLTVAAPA"
     gene            complement(1453..1725)
                     /locus_tag="VHF88_06565"
     CDS             complement(1453..1725)
                     /locus_tag="VHF88_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2161465.1"
                     /translation="MRVIGIRELRQQASRYLRDVERGETLEVTDRGRPIARVVPIPPA
                     AGLEQLAASGRLTPASGDALELGPPLAPRGKVLPSDVLADLRSGER"
     gene            complement(1772..2236)
                     /locus_tag="VHF88_06570"
     CDS             complement(1772..2236)
                     /locus_tag="VHF88_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161466.1"
                     /translation="MTRKADFNAEEWSTLVEAPLLAGMRVVTAGRGGTIRESMAIGKA
                     YAAARQQHGESELLDDLVSSPPAMQPPRVGSADEVPRVVDEHLRKAVGILETKATPDE
                     LEAYRGFVVTLAEAVANAHKEGGFLGVGGTPVSDDERKALDEIAATLGSSGA"
     gene            complement(2233..3207)
                     /locus_tag="VHF88_06575"
     CDS             complement(2233..3207)
                     /locus_tag="VHF88_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161467.1"
                     /translation="MVEHEATLEDLPPLELLELRLLDLEAPSHPGRPAHVSAASGVVQ
                     RGDFAYVIGDDELFLAVFRVSDQSPGRLHRALGGDLPLGHDDRKRAKPDLEVLTALPP
                     FGSHAHGALLGLGSGSKPNRDRGFVWGLAPDGSLDGEPYEIDLEPLYARLRESVDDLN
                     VEGAAVMGDGLWLLQRGNGERGVNVVTEVALDEALDALLRDRAFVASELRAMRSYDLG
                     RLGDAKLTFSDASPLGDELLVFTASAEASPDTFADGEIAGSVVGTIGRDGEVRRLREI
                     DSDHKVEGVHAALDAGVATLTLVCDQDDAEAASPLLSATLPLDQEEKT"
     gene            complement(3251..4432)
                     /locus_tag="VHF88_06580"
     CDS             complement(3251..4432)
                     /locus_tag="VHF88_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX2161468.1"
                     /translation="MSESTLREVVTTLAPLERGAGSEGEAEAARWLAERLQAAGAPAR
                     VESVDYRAGYARLLGPLGTAATIAGAIALTGRHRRLAALIAALAGLAIADDADNRWRV
                     WRRVATRPRKTTNVVAEIGDLAAERTLVVLAHHDAAPTGWIFDQSLQRALARRFPAVI
                     ERANRSLPLWWPIVGAPLLVALGALANRRGLLATGTATGVLTGAVGADIARHRIVPGA
                     NDNLSGVAALVDLAERIRERPIDGLRVVLASCGAEEVLQGGIYDFVDRHLRSLDPART
                     SVLNLDTVGSPRLVLLEGEGVLRIEEYPGRAFRDLIAESAAGSGIPLVRDQWARSSTD
                     SVIPSRAGYPTATITSFEPETKLLSNYHLPTDTPENVDFSTVRQAVDLTEALARRLAS
                     A"
     gene            4468..>5013
                     /locus_tag="VHF88_06585"
     CDS             4468..>5013
                     /locus_tag="VHF88_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161469.1"
                     /translation="MSAFLLACGRLVALCVALLALPATATAANSAVYDDRVNDAVYPG
                     VDIKRIALAYTDDGLVGIGVSTNVLIGGNRLAVYIDSDLNDATGFNGADYALFYERYI
                     NGQSLGYWVWNGSAWQMGAPATLAYEAGDYGVNMVIHSSELGSPAAGIDVFARGGHAN
                     PLGVGTDYWDTAPNVGQWLSYA"
BASE COUNT          759 a         1784 c         1752 g          718 t
ORIGIN      
        1 ccgagctcgg cgacttcgtc gaggacgagg ccgcgctgtc cccgttcgac accgcgtcgg
       61 agaacatccg ccgcgagaac gtccgcacgg tcctcgaggc cctgcctccg cgcgagcgcg
      121 aggtcctcga gatgcgctac ggcctcaagg gccaccaggc gcgcacgctc gaggaggtcg
      181 gccgcgcctt cggcgtgacc cgcgagcgca tccgccagat cgagaacaac accctcaaga
      241 agctcgagaa cctcccggag gcccaacgcc tccgcgaggc ctcctaggac tcctggggag
      301 gagccggacg ggcgccactc ggcgcccgtc ctcgttccgg ggcgctccgg ctagaatctc
      361 cgcacgctcg gggggatgtc cgagtggtta aaggagtgcg gctgtaaacc gcatggctat
      421 gcctacgcag gttcgaatcc tgctcccccc acttgccgaa cctcaacgat ccgcagtacg
      481 acgagcagcg cgacaaggag gggtttcgcg ctcgacgggc acggatcggc taccagctcg
      541 gtaccgagcg cctcggcatc agccagtggg agctgtcgcc cggagaggcg gcctacccgt
      601 accacctcca tctcgccgag gaggagctgc tgatcgtcct gaagggaacc ctggcgctcc
      661 gcacccccga gggctggcgt gacctcgcgg agggcgaggt cgtctcgttc ccacgcggcg
      721 aggagggagc ccaccaggtc gtcaaccgca ccgactcgac cgtcagcttc ctcgcgatca
      781 gcaccaacgg cgagccggac atcgtcctct acccggactc gaacaaggtc ggcgccagcg
      841 agcgccggcc acagggcggc ggcctacgca ccttcttccg cctcgacgac gtcgtcgact
      901 actacgaagg cgagcagcct cccaccgact gatccgcgct cgcgctaggc tgcctccagc
      961 gcgccctcgt agctcagttg gtagagcact tccatggtaa ggaaggggtc tccggttcga
     1021 gtccggacga gggctctggg aagtgcggaa gcgggctgtg gctaggccgg cgcggcgacg
     1081 gtcaagccga ggtcctcggc ggcttgtgcc atgcggtggt cgtaggtgac gagtgctgcg
     1141 aggttgctgg tgaccgtctg tgccgaggcg atgtgaatcg catcgaggct gcgtagacga
     1201 tcggcttcga gcagggcggc ggcatccagc agcgcatcgt ccaggtgcag gagggagatc
     1261 cgtgcgagta gctgacgcgc ccgtgccatc gcgcgaggtc cgtgggggcg ggtcgcgcgc
     1321 aggacctcga cgcgagcgag agcggacgag gcgcgggtcg ggtgatcgcc taggtaggcg
     1381 gcaagtgcct cggactcgct ctcggcgacg atgagcttga cgagcgccga ggagtcgagg
     1441 tagacgaccg tctcagcgct cacccgagcg aaggtcggcc agcacgtcgc ttgggaggac
     1501 cttgccccgg ggcgccaatg gagggcctag ctccagagca tccccgctcg cgggggtcaa
     1561 gcgtccgctg gcagcgagct gctcgagtcc cgcggctggc ggaatcggaa cgacgcgtgc
     1621 gatcgggcgg ccgcggtcgg tcacctcgag cgtctcgccc cgctcgacgt cacgcaggta
     1681 gcggcttgcc tgctgtcgca gctcgcggat cccgatcacg cgcatgtgct acttatagca
     1741 ttcccgacta atatggatga ggctcggagg gctaggcgcc cgacgacccg agcgtcgccg
     1801 cgatctcgtc gagcgccttg cgctcgtcgt cgctgaccgg tgtgccgccg actccgagga
     1861 agccgccctc cttgtgcgcg ttcgcgaccg cctcggcgag cgtaacgacg aagccgcggt
     1921 aggcctcgag ctcgtcgggc gtcgccttgg tctccaggat gccgacggcc ttgcgcaggt
     1981 gctcgtcgac gacgcgcggg acctcatcgg ccgagccgac ccggggcggc tgcatcgccg
     2041 gcggcgagga gacgaggtcg tccagcagct cgctctcgcc gtgctgctgg cgggcggcgg
     2101 cataggcctt gccgatcgcc atgctctcgc ggatcgtgcc gccgcggccg gcggtcacga
     2161 cgcgcatgcc ggcgagcagc ggggcctcga cgagcgtcga ccactcctcg gcgttgaagt
     2221 cggccttcct ggtcatgtct tctcctcttg gtcgagtggc aacgtggcgg acagcagcgg
     2281 cgacgcggcc tcggcgtcat cctgatcgca gacgagggtc agcgtcgcca ccccggcgtc
     2341 gagcgcggcg tgaacgccct cgaccttgtg gtcgctgtcg atctcgcgca gccggcgaac
     2401 ctcgccgtcg cgaccgatcg tcccgactac cgacccggcg atctcgccgt ccgcgaaggt
     2461 gtcgggcgac gcctcagccg aggcggtgaa gacgagcagc tcgtcgccga gcggactcgc
     2521 atcgctgaac gtgagcttgg catcgccgag ccggccgagg tcgtacgagc gcattgcccg
     2581 gagctcgctc gcgacgaacg cacggtcccg cagcagcgcg tcgagcgcct cgtcgagagc
     2641 gacctcggtc acgacgttga ccccgcgctc gccgttgccg cgctggagca gccagagccc
     2701 gtcgcccatc accgccgcgc cctcgacgtt gaggtcatcg acgctctcgc gcagccgggc
     2761 gtagagcggc tcgaggtcga tctcgtaggg ctcgccgtcg agcgagccgt ccggcgccag
     2821 cccccagacg aagccgcggt cgcggttcgg cttggacccg gagccgagcc caagcagcgc
     2881 cccgtgagcg tgcgacccga acggcggcag tgccgtcagc acctcgaggt cgggcttggc
     2941 ccgcttgcga tcgtcatggc cgagtggcag atccccgccg agcgcgcgat gaagccgtcc
     3001 cggcgactgg tcggagaccc ggaagaccgc gaggaacagc tcgtcgtcgc cgatcacgta
     3061 cgcgaagtcg ccgcgctgga cgacgccgct ggctgccgag acatgcgccg ggcggccggg
     3121 gtgcgacggc gcctcgaggt caagcagacg cagctccagc agctcgagcg gcggcaggtc
     3181 ctccagcgtc gcctcgtgct cgaccattcc cagctgcttg cccgcgcccc agcgcgagga
     3241 aacgacgcgt ctacgcgctg gccagccggc gcgccagcgc ctctgtcagg tcgacggcct
     3301 gtcgaaccgt cgagaagtcc acgttctccg gggtgtcggt cggcaggtgg tagttcgaca
     3361 gcagcttcgt ctccggctcg aacgacgtga tcgtggcggt cggatagcca gcgcggctgg
     3421 ggatcacgct gtcggtgctg ctgcgcgccc actggtcgcg aaccagcggg atgccggaac
     3481 ctgccgcgct ctcggcgatc agatcgcgga acgcccggcc cgggtactcc tcgatcctca
     3541 gcacgccctc gccctcgagc agcacgagcc gcggcgagcc gaccgtgtcg aggttgagca
     3601 ccgaggtgcg cgccggatcg agcgatcgca gatggcggtc gacgaagtcg tagatcccgc
     3661 cctggagcac ctcctcggcg ccgcacgagg cgaggaccac gcgcagcccg tcgatcggcc
     3721 gctcgcggat gcgctcggcg aggtcgacca gcgccgccac ccccgagagg ttgtcgttcg
     3781 cgcccggaac gatcctgtgg cgggcgatgt cggccccgac cgcccccgtc aaaacgcccg
     3841 tcgccgtccc ggtcgccagc agcccgcggc ggttggcgag cgcgccgagc gcgaccagca
     3901 gcggcgcgcc gacgatcggc caccacagtg gcagcgaccg gttcgcgcgt tcgatcacgg
     3961 ccgggaacct gcgagccagc gcacgctgca gcgactggtc gaagatccag ccggtcggcg
     4021 ccgcgtcgtg gtgcgcaagc accacgagcg ttcgctcggc ggcgagatct ccgatctcgg
     4081 ctacgacgtt cgtcgtcttg cgcggacggg tcgcgacgcg gcgccaaacc cgccaccggt
     4141 tgtcggcgtc gtcggcgatc gccaagccgg cgagggcggc gatcagcgcg gccagtcgtc
     4201 gatggcggcc cgtcagggcg atcgcgcccg cgatcgtcgc cgcagtcccg agcggcccga
     4261 gcagtcgtgc gtagccggcg cgatagtcga ccgactcgac ccgtgcgggc gcgcccgccg
     4321 cctggagccg ctccgcaagc caacgcgccg cctcggcctc gccctcggag ccggcccccc
     4381 gctcgagcgg ggcaagcgtc gtgacgacct cgcgcagggt ggactcggac attcgagcgg
     4441 aaaccctaag cgagctaggc tcgtgagatg tccgccttcc tcctcgcctg cgggcgactc
     4501 gtcgccttgt gcgtggctct actcgcgctg ccggcgaccg ccacggcggc gaactccgcc
     4561 gtctacgacg atcgagtcaa cgacgccgtc taccccggcg tggacatcaa gcggatcgcg
     4621 ctcgcctaca ccgacgacgg gctggtcggc atcggcgtgt ccacgaacgt gctgatcggc
     4681 ggcaatcggc tcgcggtcta catcgactcc gacctcaacg acgccacggg tttcaacggt
     4741 gctgactacg ccctcttcta cgagcgctac atcaacggtc agtcactggg ctactgggtg
     4801 tggaacggca gcgcatggca gatgggcgca cccgcgacgc tcgcctacga ggccggcgac
     4861 tacggcgtca acatggtcat ccacagctcg gagctgggct ccccggcagc cgggatcgac
     4921 gtcttcgccc gcggcggcca cgcgaatccg ctcggcgtgg ggaccgacta ctgggacacg
     4981 gcgccgaacg tcggccagtg gctgagctac gcc
//
LOCUS       DATABV010000150         4667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2697242, whole genome shotgun sequence.
ACCESSION   DATABV010000150 DATABV010000000
VERSION     DATABV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697242"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3765
                     /locus_tag="VHF88_06590"
     CDS             <1..3765
                     /locus_tag="VHF88_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161470.1"
                     /translation="HAYGSYAFGDPATPMPRSYQGDPTKMRILHGGSETFHVYHLHGG
                     AIRWRFNPHADTTFDYQDTGLNKHPAVAQSPSSRLDSQSTGPGQSFDLQVENGAGGGQ
                     AGAGEFLFHCHIAEHYVAGMWGFWRVFDTLQPHLAPLPDREPPPEAVDSAELIGKTMP
                     DGTTLTKDNIDAWIRPQLPPQGVPSGEQDATVWNWQVDDSDPENPLYLGEPEFRDPSW
                     PNLTNALDEQKLPGHPGLFPGDEPVGPQDRPKILFNPENGRPAFPLLRPQIGDRPPFS
                     PNGHSGAPYLGEQGDLPKQGDGPDPWANREDGICPAGSPRRHFNVVALDVSVPVTRNV
                     TDRTGKIFVLAKDADDVAAGRKQAEPLAIRGNQGDCVSLTLTSRQRDENASDRYAKVN
                     MHIHHVQFDTQASDGVTSGMSYEQSVRPYAAEDPRLTAAANTGATTLSLTDVSKFQPG
                     VAIGVGLGTEAIEIRTIESIDAAQRQVTLDRPLQNDHPSGRTAGVEFAQYRWYPDAML
                     DNVFWHDHVDGIHGWGHGLVGQFIVEPPGSTYHDPRTGAEIESGAIADIRTNNPMAAA
                     AGVKGSFREMVTWTIDENPVTDSTINLRAEPFADRYARNSDPSLLFSSWTHGDPVTPL
                     PMAYANDPFVIRAISVSSDGESFHVDGHRFNLETRWPKSTSLSTMTYGASEKASLILE
                     GGAGGPQGRPGDYLYMNGVGRRFRQGAWGMIRVLPKQVSQLQPLPGTNVPLGGAPLPT
                     PTGGRPPASAATGNPCPSGAPERHFKVAAVDRPLGSPLGTLAAFVPAADADAVESGRL
                     RPEPLVMHAAAGECIVAQLENRRGQRASLHADELVRSAESSGVNVGYGPEQTVAPGQS
                     RVYRYYADTKDIGSAQLSDYGDVDSGRDGLYGAIVVADKGAKFFDSSTGAPTSIGSQV
                     DVHLPNGDAYRDFTLNLADDDPVIGGSNMPYPSAVDRPALVNHRSARRPDSPTQFSSA
                     VRGDPATPILRAHAGDRVRVHALGAPGNEQTHVLSMGGQSFAIDPLIPRSNEVGSHLV
                     GPRQSLDFEIEGAGGRNHATGDFFYGDLRRAFTEAGMWGLMRVTPDEGCNPMPLHDDR
                     CPRPPAPPEDLVPEGDVGPRDDTRAQVQTPTQPKARQNKSSGDAATPIVRKPTGLRVA
                     KRVKLSKLAKRGIRLRFRAPAGSRVVRLRLLQLDSEGVANVIAKRVLAVKGGKKTRVT
                     WRLRQRELRRMRGGRYVVELRAGKRSSSLWAARVERPIVVFGPVVPASKR"
     gene            3783..>4667
                     /locus_tag="VHF88_06595"
     CDS             3783..>4667
                     /locus_tag="VHF88_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161471.1"
                     /translation="MLRFLAAALGLSALALAGPASAATDLSGSPIPNTSTLSETAQLE
                     GDPSLLRLLAAPSSPDDDDAAALAALGAPDGPEEDLAAALDRLAAAPTAADAAAARRF
                     ALDVLEGAPLDRAYSGIPLLNWNSPAKVKDVEAGGTVTVRQVRFGDHVVSDTWLLRFA
                     DPRQPFEIAYEVTELGGAFGGELTPTPLLADGTTPVGGMNAAIAPLALPELETGTLTT
                     SRFHPHGADEHSRLGTQRIVVRMPPPRNVAAILDPSRAAGLQDRTTTGEIPTATRALA
                     TLAPATDDRLAAAVARAGF"
BASE COUNT          702 a         1848 c         1553 g          564 t
ORIGIN      
        1 cacgcctacg gctcctacgc cttcggcgac cccgcgacgc cgatgccgcg cagctaccag
       61 ggcgacccga ccaagatgcg gatcctccac ggcggctcgg agaccttcca cgtctaccac
      121 ctgcacggcg gcgcgatccg ctggcgcttc aacccgcacg ccgacacgac gttcgactac
      181 caggacaccg ggctgaacaa gcacccggca gtcgcccagt cgccgtcgtc gcggctcgac
      241 tcccagtcga ccggtccggg tcagtccttc gacctccagg tcgagaacgg cgccggcggt
      301 ggccaggcgg gagcgggcga gttcctcttc cactgccaca tcgccgagca ctacgtcgcc
      361 ggcatgtggg gcttctggcg cgtcttcgac acgctgcagc cgcacctcgc gccgctgccc
      421 gaccgcgagc cgccgcctga ggcggtcgac tcggccgagc tgatcggcaa gacgatgccc
      481 gacggcacga cgctgaccaa ggacaacatc gacgcctgga tccggccgca gctgccaccg
      541 caaggcgtcc cgagcggcga gcaggacgcg accgtctgga actggcaggt cgacgacagt
      601 gaccccgaga acccgcttta cctcggcgag cctgagttcc gcgacccgag ctggccgaac
      661 ctgaccaacg cgctcgacga gcagaagctg ccgggacacc cgggcctgtt ccccggcgac
      721 gagccggtcg ggccccagga ccgcccgaag atcctcttca accccgagaa cggccgcccc
      781 gccttcccgc tgctgcggcc gcagatcggc gaccgtccgc cgttctcgcc gaacggccac
      841 tccggcgcgc cgtacctcgg cgagcagggc gacctgccca agcagggcga cggaccggac
      901 ccgtgggcga accgcgagga cggcatctgc ccggccggct cgccgcgccg ccacttcaac
      961 gtcgtcgcgc tcgacgtgtc cgtcccggtg acgcgcaacg tcaccgaccg caccggcaag
     1021 atcttcgtgc tcgccaagga tgccgacgac gtcgccgccg gacgcaagca ggccgagccg
     1081 ctcgcgatcc gcggcaatca gggcgactgc gtcagcctga ccctgaccag ccgccagcgg
     1141 gacgagaacg cctccgaccg ctacgcgaag gtcaacatgc acatccacca cgtgcagttc
     1201 gacacgcagg cctccgacgg cgtgaccagc ggcatgtcct acgagcagtc ggtgcgcccg
     1261 tacgcggctg aggacccgcg cctgacggcc gccgcgaaca ccggcgcgac gacgttgtcg
     1321 ctgaccgacg tctcgaagtt ccagccgggc gtcgcgatcg gcgtcggcct cgggaccgag
     1381 gcgatcgaga tccgcacgat cgagtcgatc gacgcagcgc agcgccaggt cacgctcgac
     1441 cggccgctcc agaacgacca cccgtccggc aggaccgcgg gcgtcgagtt cgcccagtac
     1501 cgctggtacc ccgacgcgat gctcgacaac gtcttctggc acgaccacgt cgatggcatc
     1561 cacggctggg gccacggcct cgtcggccag ttcatcgtcg agccgcccgg atcgacctac
     1621 cacgacccgc gcaccggagc cgagatcgag tccggcgcga tcgccgacat tcgcaccaac
     1681 aacccgatgg ccgccgcggc cggggtcaag ggcagcttcc gcgagatggt cacctggacg
     1741 atcgacgaga acccagtcac cgactcgacg atcaacctgc gcgccgagcc gttcgccgac
     1801 cgctacgcac gcaacagcga cccgtcgctg ctgttcagct cctggacgca cggcgacccg
     1861 gtcacgccgc tgccgatggc ctacgcaaac gacccgttcg tgatccgcgc gatcagcgtc
     1921 tcctccgacg gcgagagctt ccacgtcgac ggccaccgct tcaacctcga gacgcgctgg
     1981 ccgaagtcga cctcgctcag cacgatgacc tatggcgcct cggagaaggc gagcctgatc
     2041 ctcgagggcg gcgccggcgg tccgcagggc cggccgggcg actacctcta catgaacggc
     2101 gtcggccgcc gcttccgcca gggcgcctgg gggatgatcc gcgtgctgcc gaagcaggtc
     2161 tcccagctgc agccgctgcc cggtaccaac gtgccgctcg gcggcgcgcc gctgccgacg
     2221 ccaaccggcg gccgtccgcc ggcctccgcc gcgaccggca acccgtgccc gagcggggcg
     2281 cccgagcgcc acttcaaggt cgccgcggtc gaccgcccgc tgggctcgcc gctcggcacg
     2341 ctggcggcct tcgtcccggc cgctgacgcc gacgccgtcg agtccggcag gctgcgcccc
     2401 gagccgctgg tcatgcacgc cgccgccggc gagtgcatcg tcgcccagct cgagaaccgc
     2461 cgcggccagc gcgcgtcgct gcacgccgac gagctcgtcc gctcggccga gtcgtccggc
     2521 gtaaacgtcg gctacggccc ggagcagacg gtcgcgcccg gccagtcgcg cgtctaccgc
     2581 tactacgccg acaccaagga catcggcagc gcccagctgt ccgactacgg cgacgtcgac
     2641 agcggccgcg acggcctcta cggcgcgatc gtggtcgccg acaagggcgc gaagttcttc
     2701 gactcgagca ccggcgctcc gacgagcatc ggctcgcagg tcgacgtcca cctgcccaac
     2761 ggcgacgcct accgcgactt cacgctcaac ctcgccgacg acgaccccgt gatcggcggc
     2821 agcaacatgc cgtacccgag cgcggtcgac cgcccggcgc tggtcaacca ccgctccgcg
     2881 cgccggcccg actcgccgac ccagttcagc agcgcggtgc gcggcgaccc cgcgacgccg
     2941 atcctgcgcg cccacgccgg cgacagggtg cgcgtccacg cgctcggcgc gcccggcaac
     3001 gagcagaccc acgtgctcag catgggcggc cagtcgttcg cgatcgaccc gctgatcccg
     3061 cgctcgaacg aggtcggctc gcacctcgtc ggcccgcgcc agtcgctcga cttcgagatc
     3121 gagggcgccg gcggccgcaa ccacgcgacc ggagacttct tctacggcga cctgcgccgc
     3181 gccttcaccg aggccggcat gtggggcctg atgcgcgtca cgcccgatga gggctgcaac
     3241 ccgatgccgc tccacgacga ccgctgcccg cgaccgccgg cgcccccgga ggacctggtg
     3301 ccggagggcg acgtcggacc tcgcgatgac acccgggctc aggtccagac gccgacgcag
     3361 ccgaaggcga ggcagaacaa gtcgtcgggc gacgccgcca cgccgatcgt ccgcaagccg
     3421 accggcctgc gcgtcgccaa gcgcgtgaag ctcagcaagc tcgccaagcg cggaatccgc
     3481 ctgcgcttcc gcgcgccggc cggcagccgc gtggtgcgcc tgcggctgct gcagctcgac
     3541 tccgagggcg tcgcgaacgt gatcgctaag cgcgtcctcg ccgtcaaggg cggcaagaag
     3601 acgcgcgtca cctggcgcct gcgtcagcgc gagctgcgcc gaatgcgcgg cggccgctat
     3661 gtggtcgagc tgcgtgccgg caagcgctcg agctcgctct gggccgcgcg cgtcgagcga
     3721 ccgatcgtcg tcttcgggcc ggtcgtgccg gcgagcaagc gctgagcggt ggtggggcgg
     3781 cgatgctgcg cttcctcgcg gcggcgctcg ggctgagcgc gctggcgctc gctgggcccg
     3841 cctcggccgc gaccgacctc tccggctctc ctatcccgaa cacctcgacg ctgtccgaga
     3901 ccgcgcagct cgagggcgac ccgtcgctgt tgcgactgtt ggcggcgccg tcgtcgccgg
     3961 acgacgacga tgcggcagcg ctggccgcac tcggcgcgcc ggacggcccg gaggaggacc
     4021 tcgccgccgc gctcgatcgg ctcgccgcgg cgccgaccgc ggccgacgcc gccgccgcgc
     4081 gccgcttcgc gctcgacgtg ctcgagggag cgccgctcga ccgcgcctac agcgggatcc
     4141 cgctgctcaa ctggaactcg ccggcgaagg tcaaggacgt cgaggccggt ggcaccgtca
     4201 cggtccgcca ggtccgcttc ggcgaccacg tcgtcagcga tacctggctg ctgcgcttcg
     4261 ccgacccgcg ccagccgttc gagatcgcct acgaggtgac cgagctcggc ggcgccttcg
     4321 gcggcgagct gacgccaacg ccgctgctcg ccgacggcac cacgccggtc ggcggcatga
     4381 acgcggcgat cgcgccgctc gcgctgcccg aactcgagac cggcacgctc acgaccagcc
     4441 gcttccatcc ccacggcgcc gacgagcaca gccggctcgg tacccagcgg atcgtcgtca
     4501 ggatgccgcc gccgcgcaac gtcgccgcga tcctcgaccc gagccgcgcc gccggtctcc
     4561 aagaccgcac gacgaccggc gagatcccga ccgcgacgcg ggcgctcgcg acgctggcgc
     4621 ccgcgaccga cgatcggctc gcggcggccg tcgcccgggc tgggttc
//
LOCUS       DATABV010000151         8442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_689033, whole genome shotgun sequence.
ACCESSION   DATABV010000151 DATABV010000000
VERSION     DATABV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689033"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..178)
                     /locus_tag="VHF88_06600"
     CDS             complement(32..178)
                     /locus_tag="VHF88_06600"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX2161472.1"
                     /translation="MPEGYCVKERKKVEIKDAKQVTMKNGRPAIQGVCPDCGTKIFKI
                     GKLE"
     gene            395..823
                     /locus_tag="VHF88_06605"
     CDS             395..823
                     /locus_tag="VHF88_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161473.1"
                     /translation="MSQRPLELILARNLMSALSTPAFLVDEGGLLVFYNEAAGMLLGK
                     RFEEVGTVGPDEWGSMFGPFDGSGEPIPYDELPLVVAVRNGRPAHAGFEIRSTDGARH
                     TVEASAFPILTAHGTRGAIAVFWPCAQDGAGPSSNGAAAR"
     gene            820..1710
                     /locus_tag="VHF88_06610"
     CDS             820..1710
                     /locus_tag="VHF88_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2161474.1"
                     /translation="MKVKLWGTRGSVPSPGPETIRYGGNTSCVGVTLSDGSLLALDAG
                     TGIRNLGLSLPDEPTRLHILLTHLHLDHIQGLVFFAPAFRPQTEIVIWGPASPEASLR
                     DRIARYISAPLSPVEVRELPCDVSFRHTPEAEWEIGSARIRAASVAHRGPTLGYRIDD
                     GDSSLAYIPDHEPALGADIETLEEDWISGFALARDASLLIHDGQYADPEYPDHLGWGH
                     SSLSHALAFARRTGAERTVLFHHDPLNSDEQLDRLGEEARERWLASGGAAERLELGVE
                     GAEYEPRPQSSPPTAAVRAI"
     gene            1779..2405
                     /locus_tag="VHF88_06615"
     CDS             1779..2405
                     /locus_tag="VHF88_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2161475.1"
                     /translation="MSSAPRIEAAAERSELVRDALSLAREAHDGQVRRTGSGEHPFIE
                     HPVAVAEHLSREGFAYEVLAAGLLHDVVEHTEVRLEDVRERFGEEVADLVGVLTESDS
                     IAPYEARKEEHRERVAAAGHEAHAVFTADKTANVETLREAYAVKGEDVDAELPVSLDL
                     KIFVWELDLEMLFRAAPELSLVDRFADEMAKLWGQRAAWARGGADGSG"
     gene            complement(2415..2900)
                     /locus_tag="VHF88_06620"
     CDS             complement(2415..2900)
                     /locus_tag="VHF88_06620"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX2161476.1"
                     /translation="MADRPGGSEESEKERLDRNLGELLQELRVALPGVQVLFAFLLAV
                     PFQQGFKEEITPFQEKVYFITLLLTAISAALLISPTAYHRMNFRRQQKKHMIFLANRL
                     AIVGIGFLALAMTGAIMLITDVLFGTTATIVFSMLALLMFVVLWGLLPLSRRLKANEG
                     P"
     gene            2935..3606
                     /locus_tag="VHF88_06625"
     CDS             2935..3606
                     /locus_tag="VHF88_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HEX2161477.1"
                     /translation="MGRLGVILGSNAVARGGAEIVAAVESAGAVALRRHGRDSYVLPH
                     LIDHGANLRSLAEAGCDRVLALGSVGSLRPEIGVGSLVCPDDFIALQLLTTTLDDARA
                     HTMPGFDRDWRSEALAAWQGAGAAPLRDGGVYRQANGPRFETPAEIRLIAMHAQLVGM
                     TLAAECVVAAEIGLPYAAVCIVDNMANGIGARPLGTEELERDRAANAATLREALAAAL
                     PRLVR"
     gene            3603..4877
                     /locus_tag="VHF88_06630"
     CDS             3603..4877
                     /locus_tag="VHF88_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2161478.1"
                     /translation="MSLALVNGQLDGEAVGLRCEDGTIAAIGPGVEPQPGDETIDAGG
                     APIVAALVNGHTHAAMTLFRGYGGDLPLMRWLREKVWPVEERLDAEDVYWGVRLACLE
                     MIRTGTARFWDMYWQPGATARAVEDAGLRATIGGPVFDLDGRTEEMKATVLRDLEELE
                     GFGTRVGRALAPHSIYAVSEGLLRWVGERAAERGLPIHIHLSETEDEVDDCVAEHGVR
                     PAFYLDRLGLLGERTLLAHGVWLDGEELELIAARGCAVVTNPVANMKLAVGGVFPYPA
                     AREAGVPVALGTDGAGSNDSLDLLADLKAFALIQRHTAGDATVLPAPEAVAIAAGAHA
                     PLLVGGSAAAPTPEPLSVGAPADFLLLRRDAHEIGVGDLASDLVYAASGSVVETTVVA
                     GRVLMRGGVVEGAAEIVAQAAERARRLGIASA"
     gene            complement(4832..6076)
                     /locus_tag="VHF88_06635"
     CDS             complement(4832..6076)
                     /locus_tag="VHF88_06635"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2161479.1"
                     /translation="MSAAVRRSFDSLSVPNYRRYFAGQIVSVSGNWMQMVAEIWLVLE
                     LTGSGVAVGATTALQFLPILLFGAWGGVLADRFAKRRLLMVTQTLMALPALALFAVTV
                     SGAVDPWMVFLVAFARGTVNAVDNPTRQAFAIEMVGAERLVNAVSLNSVLIHASRLIG
                     PAIAGVVIATAGVAPCFAVNVLTFGAMVVALGRMEPSQLQTPPRGGRERGAVRAALRY
                     VRGTPELAVPLALMALVGTFGLNFQAILPLLARFSFDGGASAYAALVAAMGAGSVVGA
                     LATGAHGRTDARLIAGSALAFGLLAALAAAMPSLALEVPIVAMLGAAAVTFAAAINSS
                     LQLTVEPHMRGRVMALYSVVFLGSTPIGAPLAGWLSEAYDPRVALLLAASSGLAAAWA
                     ARLTLGRRQAEAMPSRRARSAA"
     gene            complement(6073..6582)
                     /locus_tag="VHF88_06640"
     CDS             complement(6073..6582)
                     /locus_tag="VHF88_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2161480.1"
                     /translation="MTELMPEKKPIAEKRSPVSADLTESAARLRMAIVRTARRMRQEA
                     ASAAGGTELSPTGAAALATVERHGPLTPSELAEIERVKRPTATRTLRLLVDAGLVDRA
                     PDPDDGRSALVSVSAAGRERLRRLRGRKNAYLARRMRELPPEDVEALERAAAILEELL
                     EAQSESERR"
     gene            6756..7520
                     /locus_tag="VHF88_06645"
     CDS             6756..7520
                     /locus_tag="VHF88_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX2161481.1"
                     /translation="MDARSARIARWLNAPLLIAAALTLPAVAITESKPGGTLEDVAIA
                     LNWVIWTAFAIELVVMLAVVPDRRAWLRRNPLSLVIVLLTPPVLPPGLEGLRVLRLLR
                     LLRLLRLAQLAREVFSLEGLRYAALLALLTAVGGGAVFTAFEEPHQNLDSWDGAYWAV
                     TTMTTLGSNVYPTTTGGQIVGVAILLVGISFVALLTGAIAQRFLDPEMAEVEEGVEAG
                     AQSAEEVALRELRNAREQLRALEIAVERIVDERGGR"
     gene            complement(7538..>8442)
                     /locus_tag="VHF88_06650"
     CDS             complement(7538..>8442)
                     /locus_tag="VHF88_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2161482.1"
                     /translation="TILYTAPTAIRACMKWGPQHPEAHDLSSLRLLGSVGEPINPKAW
                     LWYHKVVGGERCPIVDTWWQTETGQIMISPLPGAVATKPGSATLPLPGIEAAVVNESG
                     EPVEEREGLLVLRRPWPGMLRTLYKEEERFVSTYFERFGPRAYLVGDAARRDEDRYFW
                     VLGRIDDVINVSGHRLSTAEVESAIVSHPKVAEAAVIGQTDADTGQAICAFVTLEGDL
                     DGSDELVAELRDHVAGKIGKLARPRRVVWSGDLPKTRSGKIMRRLLRDIAEGRELGDV
                     TTLRDPAVMGELERKIAARRDEEG"
BASE COUNT         1203 a         2994 c         3038 g         1207 t
ORIGIN      
        1 aaggcgggcg gctgacgccg ccccgccgcc tctattcgag cttgccgatc ttgaagattt
       61 tcgtaccgca atcggggcag acgccttgaa tcgccgggcg accgttcttc atggtcacct
      121 gtttggcgtc tttgatctcg accttcttgc gctccttgac gcagtagccc tcgggcattg
      181 ggtctccttt cgccgctgtc gcggtctcgc gctgggcacg aggtggattg cggcaatcta
      241 accgagcggc aagacgctaa gtgaccaagt ccacacagcg ggcccccgga gggctcgatt
      301 gtttgacgac ggggtagcgg aggaaggccg caatgccggt aatagcgcca gatccagaac
      361 ctagtgctga aaagaacgaa ctaaaatgtc ctccgtgtcc cagcggcccc tcgagctgat
      421 tctcgcccgg aacctgatgt cggcgctctc gacgccggcc ttcctggtcg acgagggcgg
      481 ccttctggtc ttctacaacg aggccgccgg gatgctgcta ggcaagcggt tcgaggaggt
      541 cgggacggtc gggccggacg agtggggatc gatgttcggg ccgttcgacg ggagcggcga
      601 gccgatcccc tacgacgagc tgccgctggt cgtcgcggtg cgcaacggcc gccccgccca
      661 cgccggcttc gagatccgct cgaccgacgg cgcccgccac acggtcgagg cctccgcctt
      721 cccgatcctc accgcgcacg ggacgcgcgg cgcgatcgcc gtcttctggc cgtgcgcgca
      781 ggacggcgcc ggcccgtcgt cgaacggagc ggcggcgcgg tgaaggtcaa gctctggggg
      841 acgcgtggtt cggtcccgtc cccagggccc gagacgatcc gctacggcgg caacacctcc
      901 tgcgtgggcg tcaccctctc cgacggctcg ctgctcgcgc tcgacgcggg aaccggaatc
      961 cgcaacctcg gcctgtcgct gcccgacgag ccgacccggc tccacatcct gctcacccat
     1021 ctgcacctcg accacatcca gggcctcgtc ttcttcgcgc ccgccttccg gccgcagacc
     1081 gagatagtca tctgggggcc ggcctcgccc gaggcctcgc tgcgcgaccg gatcgcccgc
     1141 tacatctcgg cgccgctctc gccggtcgag gtgcgtgagc tgccctgcga cgtctccttc
     1201 cggcacacgc cggaggccga gtgggagatc ggctcggcgc ggatccgggc cgcctccgtc
     1261 gcccatcgcg ggccgacgct cggctaccgg atcgacgacg gcgactcctc gctcgcctac
     1321 atccccgacc acgagcccgc cctcggcgct gacatcgaaa cgctcgagga ggactggatc
     1381 tccggcttcg cgctcgcccg cgacgcgtcg ctgctgatcc acgacggcca gtacgccgac
     1441 cccgagtacc ccgaccatct cggttggggg cattcctccc tctcgcacgc gctcgccttc
     1501 gcgcgacgaa ccggcgccga gcgcacggtc ctcttccacc acgacccgct gaactccgac
     1561 gagcagctcg accgactcgg cgaggaagcg cgcgagcgct ggctggccag cggcggcgcc
     1621 gccgagcggc tcgagctcgg cgtcgaggga gccgagtacg agccgcggcc acagagctcg
     1681 ccgccgacgg ccgccgtacg agcgatctag cgatcgttgt cctgattcgg tacgaccggg
     1741 tcaagttttt tatctaatcg cgctaccctc gggcgcggat gtcctccgct ccacggatcg
     1801 aagcggcggc cgagcgttcc gagctggttc gcgacgcact gtccctggcg cgcgaggcgc
     1861 acgacggcca ggtgcgccga accgggagcg gcgagcaccc cttcatcgag catcccgtcg
     1921 ccgtcgccga acacctgtcg cgcgaggggt tcgcgtacga ggtgctcgcc gccggcctgc
     1981 tgcacgacgt cgtcgagcac accgaggtca ggctggagga cgtgcgggag cgctttggcg
     2041 aggaggtggc ggacctggtg ggcgtcttga cagaaagcga ctcgatcgcc ccctacgagg
     2101 cccgcaagga ggagcaccgc gagcgggtcg cggcagcggg tcacgaggcg cacgccgtct
     2161 tcaccgccga caagacggcg aacgtcgaaa cgctgcgcga ggcctacgcc gtaaagggcg
     2221 aggatgtcga cgccgagctg ccggtctcgc tcgacctgaa gatcttcgtc tgggagctgg
     2281 acctcgaaat gctcttccgc gccgcgccgg agctgtcgct cgtcgaccgc ttcgccgacg
     2341 aaatggcgaa gctgtggggg cagcgcgccg cctgggcccg cggcggcgcg gacggcagcg
     2401 gctaggaccc gctcctaggg cccttcgttg gccttgagcc gccggctcag cggcagcagg
     2461 ccccagagca ccacgaacat caggagggcc agcatgctga agacgatcgt cgcggtggtg
     2521 ccgaagagga cgtcggtgat cagcatgatc gcgccggtca tcgccagggc cagaaagccg
     2581 atgccaacga tcgccagccg gttggcgagg aagatcatgt gcttcttctg ctgacggcga
     2641 aagttcatcc ggtggtaggc ggttggcgaa atcagcaacg ccgctgagat ggccgtgagc
     2701 aggagggtga tgaagtagac cttctcctgg aagggcgtga tctcttcctt gaagccctgc
     2761 tggaagggca ccgcgagcag gaaggcgaag aggacctgaa cccctgggag ggcgacgcgg
     2821 agctcctgca ggagctcgcc aaggttgcgg tcgaggcgtt ccttctcgct ctcctcgctg
     2881 cccccgggac gatcggccat ggcggcacga tagtcgccac ataggatcgc cgcgatggga
     2941 cgcttgggcg tgatcctcgg ctcgaacgcc gtcgcgcgcg gcggcgcgga gatcgtcgcc
     3001 gcggtcgagt cggcgggcgc ggtcgcgctg cggcgccacg gccgcgactc ctacgtcctg
     3061 ccccacctga tcgaccacgg cgccaacctg cgctcgctcg ccgaggcggg atgcgaccgg
     3121 gtgctcgcac tcggctcggt cggctcgctg cgtccggaga tcggcgtcgg ctcgctggtc
     3181 tgcccagacg acttcatcgc cctgcaactg ctgacgacga cgctggacga cgcgcgggcc
     3241 cacaccatgc cggggttcga tcgcgattgg cgttcggagg cgctcgccgc atggcagggc
     3301 gccggcgcgg cgccgttgcg cgacggcggc gtctaccggc aggcgaacgg cccgcgcttc
     3361 gagactccgg cggagatccg gctgatcgcc atgcacgccc agctggtcgg gatgacgctg
     3421 gcggccgagt gcgtggtcgc ggccgagatc ggcctcccct acgcagcggt ctgcatcgtc
     3481 gacaacatgg cgaacgggat cggcgccagg ccactcggca ccgaggagct cgaacgcgat
     3541 cgcgccgcca acgcggcgac gctgcgcgag gcgctcgccg cggcactgcc gagactggtc
     3601 cggtgagcct cgcactcgtc aatgggcagc tcgacggcga ggcagtcggg ctgcgctgcg
     3661 aggacggcac gatcgccgcg atcggcccgg gggtcgaacc acagcccggg gacgagacga
     3721 tcgacgcggg cggggcgccg atcgtcgccg cgctggtcaa cgggcacacg cacgccgcga
     3781 tgacgctctt ccgcggctac ggcggcgatc tgccgctgat gcgctggctg cgcgagaagg
     3841 tctggccggt cgaggagcgg ctcgacgccg aggacgtcta ctggggcgtc cgcctcgcct
     3901 gcctggagat gatccgcacc ggcaccgcgc gcttctggga catgtactgg cagccggggg
     3961 caacggcgcg ggcggtcgag gacgccggcc tgcgggcgac gatcggcggg ccggtcttcg
     4021 acctcgacgg cagaaccgag gagatgaagg cgacggtgct gcgcgacctc gaggagctgg
     4081 aggggttcgg cacgcgagtc ggccgtgccc tcgcccccca ctcgatctac gcggtcagcg
     4141 aggggctgct gcgctgggtc ggcgagcggg ctgccgagcg cggactgccg atccacatcc
     4201 acctctccga gaccgaggat gaggtcgacg attgcgtggc ggagcacggc gtgcgtcccg
     4261 ccttctacct cgaccggctc ggcctgctcg gcgagcggac gctgctcgcg cacggcgtct
     4321 ggctggacgg cgaggagctg gagctgatcg ccgcccgcgg ctgcgccgtc gtcaccaacc
     4381 cggtggcgaa catgaagctc gcggtcggtg gcgtctttcc ctacccggcg gcgcgcgagg
     4441 cgggcgtccc ggtggccctt ggcaccgacg gcgcgggctc caacgactcg ctcgacctac
     4501 tcgccgacct gaaggcgttc gcgctcatcc agcgccacac cgccggcgac gcgaccgtgc
     4561 tccccgcgcc cgaggcggtg gcgatcgccg cgggcgccca cgcgccgctg ctcgtcgggg
     4621 gatcggccgc ggcgccaacc cccgagccgc tcagtgtcgg cgcgccggcc gacttcctgc
     4681 tgctgcgtcg cgacgcccac gagatcggcg tcggcgacct ggcctcggac ctcgtctacg
     4741 ccgccagcgg ctcggtggtc gagacgaccg tggtcgccgg tcgggtgctg atgcgcggcg
     4801 gggtcgtcga gggcgccgcg gagatcgtcg ctcaggccgc cgagcgggcc cggcggctcg
     4861 gcatcgcctc ggcctgacgg cggccgagcg tgaggcgagc cgcccaggcg gcggccagcc
     4921 cggagctggc ggccaacagc agcgcgacgc gcgggtcgta ggcctccgat agccagccgg
     4981 cgagcggcgc cccgatcggc gtcgagccga ggaagacgac cgagtagagc gccatcacgc
     5041 gaccgcgcat gtgcggctcg accgtgagct gtagggatga gttgatcgcc gcggcgaagg
     5101 tcacggccgc ggcgccgagc atggcgacga tcggcacctc gagcgccagg ctcggcatcg
     5161 ccgcggccag cgccgcgagc aggccgaagg cgagcgccga gccggcgatc aggcgcgcgt
     5221 cggtgcgacc gtgggcgccg gtggcgagcg cgccgaccac cgagccggcg cccatcgcgg
     5281 cgaccagcgc cgcgtaggcg gaggcgccgc cgtcgaagct gaagcgggcg agcagcggca
     5341 ggatcgcctg gaagttgagg ccgaaggtgc cgaccagcgc cattagggcc agcggcacgg
     5401 ccagctccgg agtgccgcgc acgtagcgca gcgcggcacg caccgcgccg cgctcgcggc
     5461 cgccgcgtgg cggcgtctgc agctgcgacg gctccatccg ccccaacgcg acgaccatcg
     5521 cgccaaaggt gaggacgttg accgcgaagc agggggcgac gccggcggtg gcgatcacga
     5581 ctccggcgat cgccgggccg atcagccgcg aggcgtggat caagacgctg ttgaggctga
     5641 ccgcgttgac gagccgctcg gcgccgacca tctcgattgc gaatgcctgc cgggtcgggt
     5701 tgtcgacggc gttcacggtg ccgcgcgcga aggcgacgag aaagaccatc caggggtcca
     5761 ccgcgccgct cacggtcacc gcgaagaggg cgagcgcggg cagcgccatc agcgtctgcg
     5821 tgaccatcag caggcggcgc ttggcgaagc ggtcggcgag cacgccgccc caggccccga
     5881 agagcaggat cggcaggaac tgcagcgccg tggtcgcgcc gaccgcgacg ccgctgcccg
     5941 tcagctccag aacgagccag atctcggcga ccatctgcat ccagttgccc gagaccgaga
     6001 cgatctgccc cgcgaagtag cggcggtagt tggggaccga gagcgagtcg aagctgcgcc
     6061 taaccgcggc gctcatcggc gctcgctctc cgactgcgcc tcgagcagct cctcgaggat
     6121 cgcggcggcg cgctccagcg cttcgacgtc ctcgggcggc agctcgcgca tgcgccgcgc
     6181 caggtaggcg ttcttgcggc cgcgcagccg gcgcagccgc tcgcgcccgg cggcgctgac
     6241 gctgaccagg gcgctgcggc cgtcgtcggg atcgggcgcg cggtcgacca ggccggcgtc
     6301 gacgagcagg cgcagcgtgc gggtggcggt cggccgcttg acgcgctcga tctcggccag
     6361 ctcgctcggc gtcagcggcc cgtgccgctc gaccgtagcc agtgccgccg ccccggtcgg
     6421 gctcagctcc gttccgccgg cggcgctcgc ggcctcctgt cgcatccgcc gcgccgtgcg
     6481 gacgatcgcc atccgcagcc gggcggcgga ttcggtcagg tccgccgaga cgggcgatct
     6541 cttctcagcg atgggcttct tctctggcat cagctcggtc attgattagg ctgggtaatt
     6601 agtctagcga attagtttcc ccggccgatg ggccggtttc cgccgccagg agtcctgtct
     6661 tggctccgtc ggcgggtggg agctgggcgg gacgcgtcga actatccgcc gtggcgccga
     6721 aaccgactga gggaaccgcc agctacccgc ccgggatgga cgcccgcagc gcgcggatcg
     6781 cccgctggct caacgcgccg ctgttgatcg ccgcggcgct gacgctgccc gcggtcgcga
     6841 tcaccgagtc gaaacccggc gggacgctcg aggacgtcgc gatcgctctc aactgggtca
     6901 tttggaccgc cttcgcgatc gagctggtcg tcatgctcgc cgtcgtcccc gaccgtcgcg
     6961 cctggctgcg gcgcaacccg ctcagcctcg tgatcgtctt gctcacgccg cccgtgctgc
     7021 cgccggggct cgaaggcctg cgcgtgctgc gactgctgcg actgctgcgg ctgctgcggc
     7081 tcgcccagct cgcccgcgag gtcttctcgc tggagggcct gcgctacgcg gctctgctcg
     7141 cgctgctgac tgcggtcggc ggcggcgcag tcttcaccgc cttcgaggag ccgcaccaga
     7201 acctcgactc ctgggacggc gcctactggg cggtgaccac gatgacgacg ctcggctcca
     7261 acgtctaccc gacgacgacc ggggggcaga tcgtcggcgt ggcgatcctc ctggtcggca
     7321 tcagcttcgt cgccctgctc accggcgcga tcgcacagcg cttcctcgac cccgaaatgg
     7381 ccgaggtcga ggaaggggtg gaggccgggg cgcaatccgc ggaggaggtg gcgctgcgcg
     7441 agctgcgcaa cgcgcgcgag cagctacggg ccctcgagat cgccgtcgag cggatcgtcg
     7501 acgagcgcgg cgggcgttag cgctcaaggg acgcacgtca gccttcctcg tcgcgacggg
     7561 cggcgatctt gcgctccagc tcccccatca ccgccgggtc gcgcagcgtc gtcacgtcgc
     7621 ccagctcgcg accctcggcg atgtcgcgca gcaggcggcg catgatcttg ccggagcggg
     7681 tcttcggcag gtcgccggac cagacgaccc gcctcggccg cgccagtttc ccgatcttgc
     7741 cggcgacgtg gtcgcgcagc tcggcgacca gctcgtcgct gccgtcgagg tcgccctcga
     7801 gggtgacgaa ggcacagatc gcctggccgg tgtcggcgtc ggtctggccg atcaccgcgg
     7861 cctccgcaac cttcgggtgg gagacgatcg cggactcgac ctcggcggtc gagaggcggt
     7921 ggccggagac gttgatgacg tcgtcgatcc gccccagcac ccagaagtag cggtcctcgt
     7981 cgcggcgcgc cgcatcgccg accaggtatg cgcgggggcc gaagcgctcg aagtaggtgg
     8041 agacgaagcg ctcctcctcc ttgtagaggg tgcgcagcat tcccggccag ggacggcgca
     8101 ggaccagcag cccctcgcgc tcctcgaccg gctcgccgct ctcgttcacg acggcggcct
     8161 cgatgccggg cagcggcagc gtcgccgagc cgggcttggt cgcgaccgcc ccgggcagcg
     8221 gcgagatcat gatctggccg gtctccgtct gccaccaggt gtcgacgatc ggacagcgtt
     8281 cgccgccgac gaccttgtgg taccagagcc acgccttcgg gttgatcggc tcgccgaccg
     8341 agccgagcag gcgcagcgag gagaggtcgt gggcttcggg gtgctgcggg ccccacttca
     8401 tgcaggcgcg aatcgccgtc ggcgccgtgt agaggatcgt ca
//
LOCUS       DATABV010000152        22583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1895636, whole genome shotgun sequence.
ACCESSION   DATABV010000152 DATABV010000000
VERSION     DATABV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22583
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895636"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VHF88_06655"
     CDS             <1..319
                     /locus_tag="VHF88_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2161483.1"
                     /translation="KRAGFARALVLDPQIVLFDEPDSGLDPVRTALLCELIQEVHAEN
                     GGAYVVITHDIMSARRVADYLAVLWKGRIVESGPASELFNSPNQFVRQFLSGEARGPL
                     GME"
     gene            363..2960
                     /locus_tag="VHF88_06660"
     CDS             363..2960
                     /locus_tag="VHF88_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2161484.1"
                     /translation="MRSGELFERIVGAVARRPLVPLLAVAVVAIAGALLALRLEPSNG
                     TDSLVSKGSTAFEQTERFKRDFGDDAVVVLVEGDLQRTLLTTDLQRLIQFEGCLGGNA
                     PREVLRTLPEPCRELAELDPAKVVLGPGTFVNTSVEAIGGELTRRLAAAREGGPEAVD
                     KVQRQLMALALRYGITDIPAVDSPSFVSALAFEDDGRTPKAQFSFLLPSDDAALIQMR
                     LKPGLTDSERSRAIDLVREAAAEPTLQPEQGARYVVTGVPVVVDGLGDAVRDAIPWLL
                     AAAILVMAATLAVVFRTRLRLLPLALALAASAITFGVLALAGGNLTMASIAVLPVLIG
                     LAVDYAIQFQARYDEVRARTGAPPAKAAPAAAAAGGPTIVTAGIATAVGFLVLLTSPI
                     PMVRGFGALLVVGIVVALVCMLLAGFALLVRYGTPRQRPDDVPPPLPRLRAAVGAFAD
                     GIVGSRTGAGIARGWRELRLRALERGERALAGAITRPGRVLAIALALAIAGWAADTQQ
                     TVVSDVRELVPADLQALRDIEALQDETGVSGEIDVVVRANDVTDPKVLAWMADFQQRV
                     LDGAGYRTGARCTQAKSPPDLCPGPSLPEILAAAGGGQRQAKDLIDAMPDYLSQGVVT
                     PDRKTANLAFGIRLQPLEQQQEIVEQIERQLDPPPGVEAAVVGLPVLAADANEQLASP
                     LRRVLTLLAALAAVFLVLLAVRRSLRDATIPLIPIALATGWSGGIMFVLGLLPGPFEV
                     ELNPMSVTLGALVIAISTEFSVLLSARYRQERGRGLSPARALEATYASTGAAVMASGA
                     TAIAGFAVLAASDIRMLRDFGILTVVDLTVSLLGVMLVLPAALVWAERRRPLEASDPD
                     GRAVVGAHA"
     gene            2953..3696
                     /locus_tag="VHF88_06665"
     CDS             2953..3696
                     /locus_tag="VHF88_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX2161485.1"
                     /translation="MPDDRFGDLGAPDRRSAAERFEEQDRINPEPDDPRRRPEAPRPD
                     NRYAWGVGIVMLMAIAVYAVTSSLPDSGEGLRGPAIGSRLPDFAAPSATGNADNGDDA
                     NVRQRSGGSAAAGPVPACQVVSPEVVNVCELRRKPLVLTFLVTRGADCEPQVDRVERM
                     REEFPQVEFASVVSGNDREEVEQIVDRRGWTMPVAVDRDGAVVNLYGIGVCPTTVFST
                     PGGTVRRVELGNLTETQLRSYIRRLVARS"
     gene            3693..4331
                     /locus_tag="VHF88_06670"
     CDS             3693..4331
                     /locus_tag="VHF88_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEX2161486.1"
                     /translation="MSGVERGWIAPELAEEFPGLDVYWLTVDARPGRTPRPVRERMNA
                     LAGRITGAHVVQSRQDSVPWSYRVLWRRLGVDPDTDRTPVEALMVERLEHGGLPSRGL
                     PNDAVVVATLETGVPIVVADAAKLSGSPGLRPATTGETVGGPEGTLRHGEVVYADEQA
                     PLARLDGQVAAARAVDDDTTAMAVWALAAAAVSQLEIEEALWTAAGMLGRAS"
     gene            4372..4857
                     /locus_tag="VHF88_06675"
     CDS             4372..4857
                     /locus_tag="VHF88_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161487.1"
                     /translation="MIDVLERQTAPAQPLRPTGAVDERPARRALQAQIERLEADLASL
                     FVASWPRKGLEFGVDLEGGRPRIRDLGELEQIRDALAERVADARRDLRARHAAEDANR
                     HLIGDMLECPERHRWVRVSNADIGEPGCHHWHVVPRWGPVGMFANWWRVKISSGCPLA
                     T"
     gene            4873..5424
                     /locus_tag="VHF88_06680"
     CDS             4873..5424
                     /locus_tag="VHF88_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161488.1"
                     /translation="MGKRSRKRADAGGGTSVAEAGGSTRAERDAARRARAERMRKGLP
                     EPAAKRGGTNRRPTLDERPPPLWAPFPLTELLVLAGIVLMGWGFLDGGGREANAKLAA
                     GLAIASLAGLELAIREHVTGFRSHTTLLAGATAIVAMLVLGLGLQLDRLGPVLITGAL
                     AFAGAFYALRQLFKRRSGGLSFR"
     gene            complement(5417..5767)
                     /locus_tag="VHF88_06685"
     CDS             complement(5417..5767)
                     /locus_tag="VHF88_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161489.1"
                     /translation="MGNIATFSAGQAQHLQALEHANKVRLARAEMKRRIAAGDLSAAE
                     VILDCPWEAESMSISDVLMSQKRWGRARCRRLLNSISIPENKRVGTLTERQRAIVAAT
                     LSGEPADDRALVAI"
     gene            complement(5945..7219)
                     /locus_tag="VHF88_06690"
     CDS             complement(5945..7219)
                     /locus_tag="VHF88_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2161490.1"
                     /translation="MAIDTDLFAKARDHDRLEQLTAAREHDLLPYFRVVEAEPGPVAL
                     MEGRERITLGSNNYLGLTGDERVKQAARDALDAYGTGLTGSRFMNGTTPLHLELEREL
                     ADWMGSEDALVFTAGYLANVGCIATLLGPDDTVICDSGDHASILDAVSMSRARIRPFR
                     HNRLDKLERMLDRAGGDGGGVLVVVDGVFSMEGDLAPIPEIVELCRRYGARLMVDEAH
                     GVGVLGANGTGACELLGAAGDVDLRMGTFSKSLASCGGFIAGPSEVIDFLRVQSRAFM
                     FTAAAVPAAVGAALGALRIIRSDEGRELMARLLDNARYLHAGLAERGFDVVAPTRLPD
                     GSELVTPIVPVIVGDDWRAVLFWKELYDAGVYANVALYPAVPRGGALLRTSVMATHER
                     EQLDRALEIFERAYALLPEEPGPAGETQKARS"
     gene            7238..8362
                     /locus_tag="VHF88_06695"
     CDS             7238..8362
                     /locus_tag="VHF88_06695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2161491.1"
                     /translation="MALEVTPVRSRRDRRAFIELPFRLHANAAQWIPPLRLERKLFLS
                     PRFNAWFKHGDAQLFLARRDGRVVGRISAHIDHNFNAYQRNDWGMFGFLELEDDPGVA
                     SALLDAAAGWLRSRGRDRMIGPMDFTMNDESGILIEGFEREPFIRQPWHPPYYQRLCE
                     GSGLQKAVDLWMWELHIEDRSKIVPGIVELAAKLEPEHGIRIRKMQRRKLRKEVDLFG
                     ETYNEAWKDNWGFVPLSKEDLDDYAVNLQLVFDRNWFMIAEKVDTGESVGIACTVPDA
                     NQLLKGTNGRLLRFLPRYLRRGQIIDRVRVGFLGVKPEYQHTGVAAGLYMEHFDMAEA
                     TRQTWGEMGWILETNEAMNRGMEGMGGKVVKRYRVYERAL"
     gene            8520..8867
                     /locus_tag="VHF88_06700"
     CDS             8520..8867
                     /locus_tag="VHF88_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161492.1"
                     /translation="MAEVGDSEVDGPEAGGELVRVGEEQVVEARPLPVAGGAESAQAI
                     ATRKVSLPATVAAASGGFVLGVAAFMLARVVRRPGAARSVSRRRRRMLAKQRGVDIAA
                     SRSFLVDVHLLKR"
     gene            8915..9763
                     /locus_tag="VHF88_06705"
     CDS             8915..9763
                     /locus_tag="VHF88_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161493.1"
                     /translation="MEVDVVPVGPYRMPGGGVDGVLRNRAGVLERLLHVKGEPATVRA
                     WVAGGAVRVRGEAASRPVAAEAVERMRFALGVDHDLRPFQKAFRRDRLLGPVIRRNPW
                     LRPRRRPEQFDAFAWAVTEQLIDVERAFRIQRAVVGRYGPRSGCGRLRDVPSAAAFAR
                     LAPAQLDACGLAPKRSIALIRAAREVASGRADLGRHEPTWQRLRTVRNIGTWTLDCLA
                     FLGQGRDDRLPAGDLAFVKWVGRAAGLGRRATEEEVRAFFEPYAPYQALAGMYALRVP
                     LTTGPV"
     gene            9809..10219
                     /gene="msrB"
                     /locus_tag="VHF88_06710"
     CDS             9809..10219
                     /gene="msrB"
                     /locus_tag="VHF88_06710"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX2161494.1"
                     /translation="MDAKVSKSDAEWREQLDPERYRVLREAGTEPPFTGAYTYSKADG
                     VYRCGACGNELFSSETKFDSGSGWPSFTEPAVADAVELREDNSLMMRRTEVVCARCGS
                     HLGHVFPDGPGPGGQRFCINSLALDLDESQAPDG"
     gene            complement(10220..11158)
                     /locus_tag="VHF88_06715"
     CDS             complement(10220..11158)
                     /locus_tag="VHF88_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161495.1"
                     /translation="MRRLLPRPWVAILLVVAVVGGGVGCGGDDSDDSSPQSLLSSAAS
                     KKIDSAEVRLSSDANVPGFPILGSRIVISGSGPFVANGPDALPEFDWNVVMRAGGQTF
                     PARVSGIDGKVYVEFMGQFYEAEEDQLAPLGLEGGPSRPRPTSLRDLGLEPDKWLTRV
                     KVDDGEEIGGDSTRLVTGAVDKRAVIDNLLAIVDTDRLSEAGDDVEGLPELDDETAGR
                     VADAVDSARVEVNVDDDGYPRRVYAKLRFTVPKDVKDTAIESGTLTFELLLEQIGDVK
                     VDVEPPFDPDPLSSLLDFAGVIFGIDEPSDLWRTPN"
     gene            complement(11199..12218)
                     /locus_tag="VHF88_06720"
     CDS             complement(11199..12218)
                     /locus_tag="VHF88_06720"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2161496.1"
                     /translation="MTTTRPRIALALTVALLFAALGAGCGEKEDVLEPADASELTLML
                     DYFPNADHAGIYAAERNGDFADAGLDVTIRQPPDPAAPIKQVAAGRVDLAISYEPEVL
                     RARDQGLRVVAVGAVVRKPLTSIISLPKAGIRRPADLAGKTVGTAGIDYQSAYLRTIL
                     VDAGVDPASVTERNVGFNFTPALLTGRVDATLGAFWNYEGTQLRLEGRKPRVIRMEEA
                     GVPTYDELVLVANESALERDGGKIRAFVSALGRGTRQLARDPERGVQPLLEANPDLDP
                     QLQRAAVAVTLPLFLPPRGRPFGWQDPDEWQAFAAWMRENRLLTEIPDAGGAFTNEFL
                     PGQGL"
     gene            complement(12215..12967)
                     /locus_tag="VHF88_06725"
     CDS             complement(12215..12967)
                     /locus_tag="VHF88_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2161497.1"
                     /translation="MKRAALTALLIATCIALWAWLASLASVDDLTLASPGEVAQAFED
                     DWSLLWDNAWVTLQEVLLGLAIAVVAGVVFAVGMHLSRALRDAAYPLLVGSQAVPIVV
                     LAPIFVLAFDYGMGPKLAIVALICFFPITVNVLDGLRAVDAEQLKLMRSFGASRLRTL
                     RTVELPAALPSFFSGLRVAATVSVIGAVFGEWAGSDAGLGRLVLLGNNQLQTPRVYAG
                     IVLLTLMAVALFALASLAERLACPWNRKESPA"
     gene            complement(12964..13764)
                     /locus_tag="VHF88_06730"
     CDS             complement(12964..13764)
                     /locus_tag="VHF88_06730"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161498.1"
                     /translation="MKVEAPPRAPLSVEATGVTLTGVTRRYATRRGASLTALADLELS
                     LTGGEVLGVVGPSGCGKSTLLELVAGLQEPDAGRVSVGERDDARERRDACALMPQRDL
                     LLPWRDALGNAGLALEARGVAARAARARAMPLFERFGLAGFERARPTALSGGMRQRVA
                     FVRTLLAGRPVLLLDEPFAALDAITRAEMQQWLADALAAEPRTVVLVTHDVEEALFLA
                     DRVAVLSPRPGRVVTELDVPYPRPRDRRAAVTDPELARLRGRALEALG"
     regulatory      complement(13759..13882)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14138..15238)
                     /locus_tag="VHF88_06735"
     CDS             complement(14138..15238)
                     /locus_tag="VHF88_06735"
                     /inference="COORDINATES: protein motif:HMM:NF022233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2600 family protein"
                     /protein_id="HEX2161499.1"
                     /translation="MPQAAHVDPTPLTSGQVRALFAAAVRELVWGLPEAARATRRWRA
                     LAETIPDEPIREDALYSLTHKRGHADGAALFSALPQRRNTVLLHLLVAYETIWDFLDN
                     VSERHTTEANGRELHLALVDALDPERPLADYYRHHPWENDGGYLRALVEFCRRSCEEL
                     PSYGRVRLLLIQESWRAQVLALNHLTDDQNRDAALRRWAAVECPGAYGATWFELSGAA
                     SASMVVHALLAIAAEANATDRDISAIYAVYWPWISLATTMLDSYVDQAEDRASGDHSY
                     ISHYPDHDCAVRRLCHCIAHAARDALQLPNGNRHAVIVGAMVAMYLSKDSARASDLRR
                     STRTLVRAGGSLVRVLLPLLRLWRIAYSQRSD"
     gene            15362..15934
                     /locus_tag="VHF88_06740"
     CDS             15362..15934
                     /locus_tag="VHF88_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2161500.1"
                     /translation="MAKLSTKHVVLGLMIERPGYGYGLQQQFSDRLGFLGLAESSVYK
                     TIERLEADGWVEAMGSRRPDSHHRATQRVLYRATPEGVVQFKRWMAQPSDRAIVRDEL
                     HAKLALSNPTDLPDLLRTAEAQSRECLAELAAIGGRSIPVSADLPWGMVATALVDDFK
                     ARWLEALVDWLDQMCRVIEERMAQGAVDAS"
     gene            15942..16673
                     /locus_tag="VHF88_06745"
     CDS             15942..16673
                     /locus_tag="VHF88_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2161501.1"
                     /translation="MPLLQLESANRSFWRGSTEIRVLRDVTLSVDQGDFVAVYGQRSA
                     GKTTLLKTAAGFEPLDAGSVTFRGEDLAAMSRRRLTRLHRDEIAWVEHAGPRSFGLSM
                     RAYVALPLLGQMGAMQAQRRAASMLEEVGVDDSGDRRWDELSDAARMLVAIAHALVRK
                     PDLLIVDDPTVGLGMADRDAIVALLRHAADDGVAILMAVPDMPAMVHADQVHSLSRGR
                     LLSVPTAARGSHGNVLEFPGRTGSS"
     gene            16673..17386
                     /locus_tag="VHF88_06750"
     CDS             16673..17386
                     /locus_tag="VHF88_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161502.1"
                     /translation="MLELRDLVKHYRSGRDETVRALDGVSMTIQAGELVALYGPSGSG
                     KTTLLSIVAALLPPDRGTVRLDGRELSSLSPKAAAHYRMHDLGYVSQAPDLLPGVPAI
                     DNAALKLFGMRMGVREAHRRAIPLMERLGLGARLGHRPDQLSAGERQRVLIARALSTG
                     PSLVLADEPTGSLDTTRSREVLTLLTEICHEQRTAMLLVTHDPRAASFASRVHALVDG
                     QVVAYEPDDLGLPAPVPQA"
     gene            17391..19928
                     /locus_tag="VHF88_06755"
     CDS             17391..19928
                     /locus_tag="VHF88_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX2161503.1"
                     /translation="MRLSNTLFLYRTRLRSRLVQELLALAGITIGVALVFAALVANSS
                     LTGSVRQLTEGIVGEADFQLVARGPIGIDERLVGEVRRLVGVEAAAPVLEARANVIGP
                     DGRRSIQLFGGDPRFVEIGGPLLEVFATDSPQEARVLSGQQGLAIPEPMTKALGILPA
                     HPFQLEIGSRRVHVPVALSLPRDAIGPLVDSPVAIAPLAYAQEIADLKGRVSRIFVKP
                     VAGRERQVERALERVAAGRANVRDADADVAIFENAAYPTNQSTTLFSVFSALVGFLFA
                     FSAMLLTAQQRRGLVADMRIAGHTTWSCVQVLLFDALVLGVLGSTLGIALGDQVSRHL
                     FDAVPGYLAFAFPLGDQRVVTWQSVAIAGGAGVLAACFALLAPLRDMTLEHPLVSRSR
                     LKLQRTQRALALAGLAALFATTVILVAYPDAAIAGIVSLTLALLLLLPLLLRSTMLVL
                     ALVVRGMKSVVPTLAILELRSRSAQTRTLAVAATGAIAVFATVSIGGAHADLQRGLDA
                     SAREIDGVADIWATFPGTPNAFATTPFEATPAMLDATERLPGVRAVRTYRGGFLDLGN
                     RRAWVLAPPAAVAMPVPPSQIEKGELDEIAARVRSGGWVTVSEAIAKDYDVGVGDSIV
                     LPTPRPTPTRVAAVTTNLGWPPGAVVLNADDYAKAWGSEAASAVHIDVAPGASPDRVA
                     GVVRQTLGAELPLRVETRAERVDRHFAAARQGLSRLTQISALVLISAILAMAIAMAGM
                     IWQRRPTVAKLKVHGYSERELWGALMVESGVLLGAGCVAGALFGLYGQLLLSRALQTI
                     TGFPVTYSAAGLTALAILALVTAVALAMLALPGWLAVRIRPTPGLTS"
     gene            complement(19948..21342)
                     /locus_tag="VHF88_06760"
     CDS             complement(19948..21342)
                     /locus_tag="VHF88_06760"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2161504.1"
                     /translation="MPASAGPAGMAPRLLELADELRREEMAVGTSELLDAFEALRHVD
                     WTDRRDFFEALATTLAKSQDDRRVLEVVFDRFFFRAAEREAVERGIKEAPAREGGGTR
                     EPELDFESLRERIRDALQDAAAPEGQLRDLARLAISAFGRQGEGSGVIGVDVQRIRRT
                     LGLRGEQAGAELPDPEAVPRERLREFEAQVRRELERQLIERTQTLPPSKPLRELDRAL
                     PTGAAQDLAQVHRVVAQLRRRLATHGRETRGRSRSSAIDVRRTMRTSLQTGGVPLDLR
                     FRPRRPRRPELYVLCDVSTSVTSASVFFLSVLHALHDSFRKLRSFVFVERISEVTDVF
                     ERERSFEAVSRAISGDAGVADVSGYTDYGRVWIEFLDRVVDDLDPRSTVIVLGDARTN
                     GREPHAAAFGQIADHAGRTFWMNPEPKLYWNYGDSVMQVYEPFCNGVFECWTTKQLEG
                     FVNAIADLRPVTSR"
     gene            complement(21362..22264)
                     /locus_tag="VHF88_06765"
     CDS             complement(21362..22264)
                     /locus_tag="VHF88_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2161505.1"
                     /translation="MTAELQSIEQTRVRLGEAGYLADEATALVSFLAGRLGKPVLVEG
                     PAGVGKTELAKALSRATGRKLVRLQCYEGLDEAKALYEWNYRKQLLRIQVEASDAGWQ
                     EVKDDIFGEEFLLPRPLLTAISAAEPVVLLIDEIDKTDQEFEAMLLEVLSDFQVSIPE
                     LGVVEASTHPIVLLTSNDSRELTEALKRRCLYLWLGYPTVERELGIVRLHAPDLSEAV
                     GRKLVEVMHMVRDLDLKKPPSIAESIDWARALLLLGAEDVDETTLKQTMSVIVKHRSD
                     LDLVAERVGVKLGDGGLVGSRPAG"
     gene            22288..22524
                     /locus_tag="VHF88_06770"
     CDS             22288..22524
                     /locus_tag="VHF88_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161506.1"
                     /translation="MSGLDPLLLLAQFLDPSRGPDQTGDFKVSPRPFLATIGAGFLIG
                     TIGHVLRSRTLVAFGVALIFGATVALPLALAIAN"
BASE COUNT         3242 a         7626 c         8209 g         3506 t
ORIGIN      
        1 caagcgcgcc gggttcgcgc gcgcgctggt gctcgacccg cagattgtcc tcttcgacga
       61 gcccgactca gggctcgacc ccgtccgcac ggcgctgctc tgtgagctga tccaggaggt
      121 ccacgcggag aacggcggcg cctacgtcgt gatcacccac gacatcatga gcgcgcgccg
      181 cgtcgcggac tacctcgcgg tgctttggaa ggggcggatc gtcgagtctg gaccggccag
      241 cgagctgttc aactcgccga accagttcgt ccgccagttc ctctccggcg aggcgcgcgg
      301 gccgctcggc atggagtagc cgcctgcgcc cggccggggc ccgctgacct aagctgagcc
      361 ccgtgcggtc gggtgagctg ttcgagcgga tcgtgggcgc cgtcgctcgg cgcccgctgg
      421 tgccgttgct tgcggttgcg gtggtggcga tcgcgggtgc cttgctcgcg ctgcggctcg
      481 agccgagcaa cgggaccgat tcgctcgtct cgaaggggtc gaccgccttc gagcagaccg
      541 agcgcttcaa gcgcgacttc ggcgacgatg cggtcgtcgt gctggtcgag ggagaccttc
      601 agcggacgct gctgacgacc gacctccagc gcctgatcca gttcgagggc tgccttgggg
      661 gcaatgcccc gagggaggtg ctgcgcaccc tgcccgagcc ctgtcgcgag ctcgcggagc
      721 tcgacccggc gaaggtggtg ctcggccccg ggacgttcgt caacacctcc gtcgaggcga
      781 tcggcggcga gctcacgcgg cggctcgcag ccgcgcgcga gggcggtccg gaggcggtcg
      841 acaaggtcca gcgccagctg atggcgctgg cgctgcgcta cggcatcacc gacattccgg
      901 cggtcgacag cccgagcttc gtctcggcgc tcgccttcga ggatgacggc aggacgccga
      961 aggcccagtt ctccttcctg ctgccgagcg acgacgcggc gctgatccag atgcgcctca
     1021 aacccgggct gacggactcc gagcgcagcc gcgcgatcga cctcgtacgt gaagcggcgg
     1081 cggagccgac gttgcagccc gagcagggtg cacgctacgt cgtgaccggt gtgccggtcg
     1141 tcgtcgacgg gcttggcgac gcggtccgcg atgcgatccc atggctgctc gccgccgcga
     1201 tcctcgtgat ggcggcgacg ctcgcggtcg tcttccggac gcggctgcgg ctgctgccgc
     1261 tcgccctcgc gctcgccgca tccgcgatca ccttcggtgt gctggcgctc gcgggcggca
     1321 acctgacgat ggcgtcgatc gcggtgctgc cggtcctgat cgggctggcg gtcgactacg
     1381 cgatccagtt ccaagcgcgc tacgacgagg tgcgcgcccg tactggtgcg ccgcccgcca
     1441 aggcggcgcc ggcggccgct gcggcaggcg ggccgacgat cgtcacggcc gggattgcca
     1501 cggcggtcgg cttcctcgtc ctgctgacgt cgccgatccc gatggtccgc ggcttcggcg
     1561 ccctgctcgt cgtcggcatc gtcgtcgcgc tcgtctgcat gctgctcgcc ggcttcgccc
     1621 tgcttgtccg ctatggaacg ccgcggcaac ggcccgacga cgtgccaccg cccctgccac
     1681 gcctgcgtgc cgcggtcggc gcgttcgcgg acggcatcgt cggttcccgg accggggccg
     1741 ggatcgcacg cggctggcgc gagctgcggc tgcgggcgct cgagcgcggc gagcgcgcac
     1801 tcgccggcgc gatcacccgc ccgggccggg tgctcgcgat cgcgctcgcg ctggcgatcg
     1861 ccggttgggc cgccgacacc cagcagacgg tcgtctccga cgtccgtgag ctggtacccg
     1921 cggatctcca ggcgctgcgc gacatcgagg cgctgcagga cgagaccggc gtctcggggg
     1981 agatcgacgt ggttgtgcgc gccaatgacg tgaccgatcc gaaggtcctc gcctggatgg
     2041 ccgacttcca gcagcgcgtg ctcgacggcg ccggctaccg cacgggcgcg cgctgcacgc
     2101 aggcgaagag cccacccgat ctctgcccgg ggccgtcgct gcccgagatc ctcgccgcgg
     2161 ccggcggggg ccagcggcag gcgaaggatc tgatcgacgc gatgcccgat tacctgtcgc
     2221 agggcgtcgt cacccccgac cgcaagacgg ccaacctcgc ctttgggatc cgtctgcagc
     2281 cgctcgagca acagcaggag atcgtcgagc agatcgagcg gcagctcgat ccgccgccgg
     2341 gcgtcgaggc ggcggtcgtc ggcttgccgg tgctcgccgc cgacgccaac gagcagctgg
     2401 cctcgccgct gcggcgcgtg ctgacgctgt tggccgcgct cgccgcggtg ttcctggtgc
     2461 tgctcgcggt ccggcgcagc ctccgtgacg cgacgattcc gctgatcccg atcgcgctgg
     2521 caaccggctg gtccggcggg atcatgttcg tgctcggcct gctgccggga ccgttcgagg
     2581 tcgagctgaa cccgatgtcg gtgaccctcg gcgcgttggt gatcgcgatc tcgaccgagt
     2641 tcagcgtgct gctctcggcg cgctaccggc aggagcgcgg gcgcggcttg tcgccggctc
     2701 gagcgctcga ggcgacgtac gcctcgaccg gcgccgcggt gatggcctcg ggggctaccg
     2761 cgatcgccgg gttcgcggtc ctggcggcgt cggacatccg gatgctgcgc gatttcggga
     2821 tcctcactgt cgtggacctc accgtctcac tgctcggagt gatgctcgtg ctgcccgccg
     2881 cgttggtgtg ggcggagcgt cgccgtccgc tcgaggcctc cgacccggac ggccgcgcgg
     2941 tcgtgggtgc ccatgcctga cgaccgcttt ggcgatctcg gagcgccgga ccgtcgcagc
     3001 gccgccgagc ggttcgagga gcaggaccgg atcaatcccg agccggacga tccacgccgg
     3061 cgcccggagg cgccgcgtcc cgacaaccgc tacgcctggg gcgtcggcat cgtgatgctg
     3121 atggcgatcg ccgtctacgc ggtcacctcg tcgcttccgg acagcggcga gggactgcgc
     3181 ggaccggcga tcggctcccg gctgcccgac ttcgctgcgc cgtcggcgac cggcaacgcc
     3241 gacaacggcg acgatgcgaa cgtccgccag cgcagcggcg gcagcgcggc agccggcccg
     3301 gtcccggcgt gccaggtggt cagtcccgag gtcgtcaacg tctgcgagct gcgccggaag
     3361 ccactggtgc tgacgttcct cgtcacgcgc ggtgccgact gcgagccgca ggtcgatcgc
     3421 gtcgagcgga tgcgcgagga gttcccgcag gtcgagttcg cctcggtggt gagcggaaac
     3481 gatcgcgagg aggtcgagca gatcgtcgac cgtcgcggct ggacgatgcc ggtcgccgtc
     3541 gaccgcgacg gtgccgtggt caacctctac ggcatcgggg tctgcccgac gacagtcttc
     3601 tcgactccgg gcggcaccgt ccgtcgggtc gagctcggca acttgaccga gacgcagctg
     3661 cgcagctaca tccggaggct ggtcgcacgg tcgtgagcgg cgtggagcgg ggctggatcg
     3721 cccccgagct ggccgaggag ttccccggtc tcgacgtcta ctggctcacc gtcgacgctc
     3781 ggcccggcag gacgccgcgc ccggttcgtg agcggatgaa cgcgctggcc gggcggatca
     3841 ccggcgccca cgtggtccag tcgcgccagg acagcgtgcc ttggtcgtac cgcgtgctgt
     3901 ggcgccggct cggcgtcgat ccggacaccg atcgcacacc ggtcgaggcg ctgatggtcg
     3961 agcggctcga gcacggtgga ctgccgagcc gcggcctgcc gaacgacgcc gtcgtcgtcg
     4021 ccacgctcga gaccggcgtc ccgatcgtcg tcgcggatgc cgcgaagctg tccggcagcc
     4081 cggggctgcg gccggcgacg accggcgaga cggtcggcgg gccggagggc acgctgcgcc
     4141 acggcgaggt cgtctatgcc gacgagcagg ctccgctcgc gcggctcgac gggcaggtgg
     4201 ccgccgctcg cgccgtcgac gacgacacga ccgcgatggc cgtctgggcg ctcgccgccg
     4261 ccgcggtgtc gcagcttgag atcgaggagg cgctgtggac cgccgccggc atgctcggac
     4321 gagcgtcgta gccgctggta gggtggaagg gtccaaaccg gggaggacgt agtgatcgac
     4381 gtactcgagc gtcagaccgc accagcacag ccacttcggc ccaccggcgc agtagacgag
     4441 cggccggcac gccgtgcgct gcaggcccag atcgagcggc tcgaggcgga cctcgcgagc
     4501 ctgttcgtgg cctcttggcc gcgcaagggc ctcgagttcg gcgtcgacct cgagggcggc
     4561 cgcccgcgga tccgcgacct cggcgagctc gagcagatcc gtgacgcgct cgccgagcgg
     4621 gtcgccgacg cccgccgcga cctgcgcgcc cgccacgctg cagaggacgc caaccgccac
     4681 ctgatcggcg acatgctcga gtgtccggag aggcaccgtt gggtacgcgt cagcaacgcc
     4741 gacatcggtg agcccggctg ccaccactgg cacgtggtgc cgcgctgggg tccggttggg
     4801 atgttcgcca actggtggcg cgtcaagatc tcatcgggct gtccgttagc cacctgacgg
     4861 tgaggtcata gggtgggcaa gcgcagccgc aagcgggcgg acgccggcgg cgggacgtcc
     4921 gtcgcggagg ccggcggcag cacccgcgcc gagcgcgacg cggcccgtcg cgcgcgcgcc
     4981 gagcggatgc ggaagggttt gccggagccg gccgccaagc gtggcggcac gaaccggcgg
     5041 ccgacgctcg acgagcgtcc gccgccgctt tgggcgccgt tcccgctgac cgagctgctg
     5101 gtgctcgccg ggatcgtgct gatgggatgg ggcttcctcg acggcggcgg tcgcgaggcc
     5161 aacgccaagc tggcggcggg tctcgcgatc gcctcgctcg ccgggctcga gctcgcgatc
     5221 cgcgagcacg tcaccggctt ccgctcccat acgacgctgc tcgccggcgc gaccgcgatc
     5281 gtggcgatgc tggtgctcgg cctcggcctg cagctcgaca ggctcggtcc cgtgctgatc
     5341 accggagcgc tcgccttcgc cggcgccttc tacgccctgc gccagctctt caagcgccgc
     5401 tccggcggcc ttagcttcag atagctacta gggcgcggtc gtcggcgggc tcgccggaga
     5461 gggtggcggc gacgatcgcg cgctggcgct cggtgagggt ccctacgcgc ttgttctcgg
     5521 ggatcgagat cgagttgagc agtcgccggc agcgcgcgcg gccccaccgc ttctggctca
     5581 tcagcacgtc gctgatcgac atgctctcgg cctcccacgg gcaatccagg atcacctctg
     5641 cagccgacag gtctccggcg gcgatgcggc gcttcatctc ggcgcgcgcg agcctgacct
     5701 tgttggcgtg ctccagcgcc tgcaggtgct gagcctggcc ggcactgaac gtagcgatgt
     5761 tgcccatacg tcctccccgc acttcgtgcg tgacttgact agcggatgca gtttcccctt
     5821 gtgtcggctc cgggctctca ccaccagcgt ccgcgccgct ggcctccttg gagggatcga
     5881 agccgagtcc gcaataaatc ggagcagccg gcgaccgtca acaggggatc gtctggctcg
     5941 cccgctaaga gcgggctttt tgggtctcgc ccgccggacc cggctcctca ggcagaagcg
     6001 catatgcacg ctcgaagatc tccagggcgc ggtcgagctg ctcgcgctcg tgggttgcca
     6061 tcacgctcgt ccgcaacagc gctccgccgc gtgggaccgc ggggtagagg gcgacgttcg
     6121 cgtagacgcc ggcgtcgtag agctccttcc agaacagcac tgcgcgccag tcgtctccga
     6181 cgatcaccgg gacgatcggc gtcacgagct ccgagccgtc gggcaaccgg gtcggcgcga
     6241 cgacgtcgaa gccgcgctcg gccaggccgg cgtgcaggta acgcgcgttg tcgagcagcc
     6301 gcgccatcag ctcgcgcccc tcgtccgagc ggatgatccg cagcgcgccg agcgccgcgc
     6361 cgacggccgc cgggaccgcc gcagccgtga acatgaacgc gcgcgactgg acgcgcagga
     6421 agtcgatcac ctcggacggt ccggcgatga agccgccgca cgacgcgagc gacttcgaga
     6481 acgtccccat ccgcaggtcg acgtcgccgg cagcgccgag cagctcgcag gcaccggtgc
     6541 cgttcgcgcc cagcacgccg acgccgtgcg cctcgtcgac catcaggcgg gccccgtagc
     6601 gacggcagag ctcgacgatc tccgggatcg gtgcgaggtc gccctccatc gagaagacgc
     6661 cgtcgacgac aaccagcacg ccgccgccat cgccgccggc gcggtcgagc atccgctcga
     6721 gcttgtcgag ccggttatgg cggaacggcc ggatccgggc gcgcgacatc gagacggcat
     6781 cgaggatcga cgcgtggtcg ccggagtcgc agatcacggt gtcatcggga ccgagcagcg
     6841 tggcgatgca gccgacgttg gcgaggtagc ccgcggtgaa caccagcgcg tcctcgctgc
     6901 ccatccagtc ggccagctcg cgctcgagct cgaggtgcag cggcgtcgtg ccgttcatga
     6961 agcgtgagcc ggtcaggcct gtcccgtagg cgtcgagggc gtcgcgagcc gcctgcttga
     7021 cgcgctcgtc gccggtcagg ccgaggtagt tgttcgaacc gagcgtgatc cgctcacggc
     7081 cctccatcag cgccaccgga cccggctccg cctcgacgac gcggaagtag ggcaacaggt
     7141 cgtgctcgcg ggcggcggtc agctgctcga gtcgatcgtg gtcgcgcgcc ttcgcgaaga
     7201 ggtcggtgtc gatcgccatt gactagggtg cggcgccgtg gccctggagg tgacccccgt
     7261 gcgatcgcgg cgcgaccggc gggcgttcat cgagctgccg ttccggctgc atgctaacgc
     7321 cgcccagtgg atccctccgc tgcgactgga gcggaagctg ttcctcagcc cacgcttcaa
     7381 cgcgtggttc aagcacggcg acgcccagct cttcctcgcc cggcgtgacg gtcgcgtcgt
     7441 cgggcgcatc agcgcgcaca tcgaccacaa cttcaacgcc taccagcgca acgactgggg
     7501 gatgttcggc ttcctcgagc tcgaggatga ccccggcgtc gcgagcgcgc tgctcgacgc
     7561 cgccgccgga tggctccggt cgcgcgggcg tgaccggatg atcgggccga tggacttcac
     7621 gatgaacgac gagtccggaa ttctgatcga ggggttcgag cgtgagccat tcatcaggca
     7681 accgtggcat ccgccgtact accagcgcct ctgcgagggg tccggtttgc agaaggcggt
     7741 cgacctctgg atgtgggagc tgcacatcga ggatcgctcg aagatcgtcc cggggatcgt
     7801 cgagctcgcc gccaagctgg agcccgagca cgggatccga attcgcaaga tgcagcgacg
     7861 caagctgcgc aaggaggtcg acctcttcgg ggagacctac aacgaggcgt ggaaggacaa
     7921 ctggggcttc gtgccgctgt cgaaggagga cctcgacgac tacgcggtca atctgcagtt
     7981 ggtcttcgac cgcaactggt tcatgatcgc ggagaaggtc gacaccgggg agagcgtggg
     8041 gatcgcctgc acggttcccg acgcgaacca gctgctgaag gggaccaacg ggcggttgct
     8101 gcggttcttg ccgcgctatt tgcgtcgggg ccagatcatc gaccgggtgc gggtggggtt
     8161 cctcggggtc aagccggagt accagcacac gggggtcgcg gcggggctgt acatggagca
     8221 cttcgacatg gcggaggcga cgcggcagac gtggggggag atggggtgga tcctggagac
     8281 gaatgaggcg atgaatcggg ggatggaggg gatggggggg aaggtggtga agcggtatcg
     8341 ggtgtacgag agggcgcttt gatcggctag ggaccgaccg tgctgcccgc gggtcctgtc
     8401 tccgattttg gttcgcgtgc tcgttcctgc gcgcgcgaac gcaaaatcgt cgccacccgc
     8461 ggaggcgggc gttggcgggt gcgtcgggtg cgtccgggcg gcggctagac tcggcgttcg
     8521 tggccgaggt gggcgacagc gaggtggacg ggcctgaggc tggcggtgag ctggttaggg
     8581 tcggggagga gcaggtcgta gaggcgcggc cgttgccggt ggcggggggc gctgagtccg
     8641 ctcaggcgat tgccacgcgc aaggtgtcgt tgccggcgac ggtggcggcg gccagtgggg
     8701 gattcgtgct gggagtcgcg gcgttcatgt tggcgcgggt ggtgcggcgg cctggggcgg
     8761 cgcggtcggt tagtcggcga aggcggcgga tgctggcgaa gcagcggggc gtggatattg
     8821 cggcctcgcg gtctttcttg gttgacgtcc atctgttgaa gcgctgactg gggcgctggc
     8881 agcggtcgag gggggagctg ccgctgcggc ggtggtggag gtcgacgtgg tgccggtcgg
     8941 gccgtatcgg atgcctgggg gcggtgtcga cggggtgttg cgaaaccgcg ccggggtgct
     9001 ggagcggtta ctgcatgtga agggcgagcc ggcgacggtg cgggcgtggg tggctggcgg
     9061 tgcggttcgg gtgcggggtg aggcggcgag tcggcccgtg gcggcggagg cggtggagcg
     9121 gatgcggttc gcgttggggg tggaccatga tctgcggccg tttcagaagg cctttcggag
     9181 ggatcggctg ctggggccgg tgattcggcg gaatccttgg ctgcggccgc ggcggcggcc
     9241 ggagcagttc gatgcgtttg cgtgggcggt gacggagcag ttgatcgacg tcgagcgggc
     9301 gtttcggatc cagcgggcgg tggtaggccg gtacggcccg cggagcgggt gtgggcggct
     9361 gcgtgacgtg ccgtcggccg ctgccttcgc tcggttggcg ccggcgcagc tggacgcctg
     9421 tgggttggcg ccgaagcgct cgatcgcgct gatccgcgcg gcgcgggagg tggcgagtgg
     9481 gcgcgcggac ttggggcggc acgagccgac gtggcagcgg ctgcggacgg tgcggaacat
     9541 cgggacgtgg acgctcgact gcctcgcgtt cctcggtcag gggcgcgacg atcggctccc
     9601 ggccggagat ctggcgttcg tcaagtgggt cgggcgggcg gcggggctcg gccgccgggc
     9661 aacggaggag gaggtgcggg ccttcttcga gccgtacgcg ccctaccagg cgctcgccgg
     9721 aatgtacgcg ctgcgtgtgc ctttgaccac tgggccggtc tgacggcgac gcctaccatt
     9781 ggaagggaac ccggatcgag gagcgcggat ggacgcgaag gtgagcaaga gcgacgcgga
     9841 gtggcgcgag cagctcgatc ccgagcgcta ccgcgtgctg cgcgaggcgg ggaccgagcc
     9901 gccgttcacc ggcgcctaca cctactccaa ggccgacggc gtctaccgct gcggcgcctg
     9961 cggcaacgag ctgttcagct cggagacgaa gttcgactcg ggcagcggct ggccgagctt
    10021 caccgagccg gcggtcgcgg acgcggtgga gctgcgtgag gacaacagcc tgatgatgcg
    10081 gaggaccgag gtcgtctgtg cgcgctgcgg ctcgcacctc ggacatgtct tcccggacgg
    10141 gcccgggccg ggcggtcagc gcttctgcat caactccctg gcgctcgacc tagacgagag
    10201 ccaggcgccg gatggctagc tagttgggag ttctccagag gtcggacggc tcgtcgatcc
    10261 cgaagatgac accggcgaag tcgagcaggc tcgagagcgg atctgggtcg aaggggggct
    10321 cgacgtcgac cttgacgtcg ccgatctgct cgagcagcag ctcgaaggtc agggtgccgc
    10381 tctcgattgc cgtgtccttg acgtccttcg gcaccgtgaa gcgcagcttg gcgtagaccc
    10441 ggcgtgggta gccgtcgtcg tcgacgttca cctcgacgcg ggccgagtcg accgcgtcgg
    10501 cgaccctgcc ggccgtctcg tcgtcgagct cgggcaggcc ctcgacgtcg tcgccggcct
    10561 ccgagagccg atcggtgtcc acgatcgcga gcaggttgtc gataaccgct cgcttgtcga
    10621 cggcgccggt gacgaggcgg gtcgagtcgc cgccgatctc ctcgccgtcg tcgaccttga
    10681 cgcgggtgag ccacttgtct ggctcgagtc cgaggtcgcg caggctcgtc ggacgcgggc
    10741 gcgagggacc gccctcgagt ccgagcggtg cgagctggtc ctcctcggcc tcgtagaact
    10801 gacccatgaa ctcgacgtag accttgccgt cgatgccgct gacgcgagcg gggaatgtct
    10861 ggccgcccgc tcgcatcacg acgttccagt cgaactctgg tagcgcgtcg ggaccgttgg
    10921 cgacgaacgg gccgctgccc gagatcacga tcctgctgcc gagaatcggg aagccgggca
    10981 cgttcgcgtc gctgctcagc cggacctcgg cgctatcgat cttcttcgat gcggcactgc
    11041 tcagcagcga ctgaggactc gagtcgtcag agtcgtcgcc tccgcaacct acgccgccgc
    11101 cgacgaccgc gacgacgagc aggatggcga cccatggccg cggaaggaga cggcgcatac
    11161 gcatcacgtc ggtttatagg ggtttggcgc gtcgtgcgtc acagtccctg gccgggcagg
    11221 aactcgttcg tgaacgcccc gcccgcgtcg gggatctcgg tcagtagccg gttctcgcgc
    11281 atccaggcgg caaacgcctg ccactcatcg gggtcctgcc agccaaatgg gcgcccgcgg
    11341 ggcggcagga aaagcggcag ggtcaccgcg acggcggcgc gctgcagctg ggggtcgagg
    11401 tcggggttgg cctcgagcag cggctgcacg ccacgctctg ggtcgcgcgc cagctgcctg
    11461 gtgccgcgcc ccagcgcgga gacgaaggcg cggatcttgc cgccgtcacg ctccagcgcc
    11521 gactcgttcg cgaccagcac cagctcgtcg taggtcggga cgccggcctc ctccatgcgg
    11581 atcacgcgcg gcttgcggcc ctcgaggcgg agctgggtgc cctcgtagtt ccagaacgcc
    11641 ccgagcgtcg cgtcgacacg gccggtcagc agcgcgggcg tgaagttgaa gccgacgttg
    11701 cgctcggtga ccgacgccgg atcgacgccg gcgtcgacaa ggatcgtgcg tagataggcc
    11761 gactggtagt cgatcccggc cgttcccacg gtcttgccgg ccagatcggc cggcctgcgg
    11821 atgccggcct tcggcagcga gatgatcgag gtcagcggct tgcgcacgac ggcgccgacc
    11881 gccacgacgc gcaggccctg atcgcgtgcg cgcaggacct cgggctcgta ggagatcgcc
    11941 aggtcgaccc gtcccgccgc cacctgcttg atcggcgccg ccgggtcggg gggctggcgg
    12001 atcgtgacgt cgaggccggc gtcggcgaag tcgccgttgc gctcggcggc gtagatgccc
    12061 gcatggtcgg cgttggggaa gtagtcgagc atcagcgtca gctcactggc gtcggccggc
    12121 tcgagcacgt cctccttctc gccgcagccg gcgcccaacg ccgcgaagag cagcgcgacg
    12181 gtgagggcca gggcgattcg cgggcgggtc gtcgtcatgc gggcgattcc tttcgattcc
    12241 atggacaggc aagccgctcg gccagcgagg cgagcgcgaa tagcgcgacc gccatcaggg
    12301 tcaggagcac gatccccgca tagacgcgcg gggtctggag ctggttgttg ccgagcagga
    12361 ccagtcggcc gagtccggcg tccgaccccg cccactcgcc gaacacggcg ccgatcaccg
    12421 acacggtggc ggcgacgcgc agcccggaaa agaagctcgg cagcgcagcc ggcagctcga
    12481 cggtgcgcag cgtgcgcagg cgcgaggcgc cgaacgagcg catcagcttg agctgctcgg
    12541 cgtcgacggc gcgcaggccg tcgagcacgt tgacggtgat cgggaagaag cagatcagcg
    12601 cgacgatcgc cagcttcggc cccatcccgt agtcgaaggc gagcacgaag atcggcgcga
    12661 gcacgacaat aggcaccgcc tgcgagccga cgagcagcgg ataggcggcg tctcgcaagg
    12721 cgcggctgag gtgcatgccg accgcgaaca ccacgccggc gacgaccgcg atcgccagac
    12781 ccagcaggac ctcttgcagc gtcacccagg cgttgtccca gagcaggctc cagtcatcct
    12841 cgaacgcctg ggccacctcg cccggcgagg cgagcgtgag gtcgtcgacg gacgcgagcg
    12901 aggccagcca ggcccacaac gcgatgcagg ttgcgatcag taatgcggtc agggcggcgc
    12961 gcttcacccg agcgcctcca gggcgcgacc acgaagccgc gccagctccg gatcggtgac
    13021 ggctgcgcgg cggtcgcgcg gccggggata gggcacgtcc agctccgtca cgacccggcc
    13081 cgggcgtggt gagagcacgg cgacgcgatc cgcgaggaag agcgcctcct ctacgtcgtg
    13141 ggtcaccagc acgaccgtgc gcggctcggc tgcgagcgcg tcggcgagcc actgctgcat
    13201 ctcggcgcgg gtgatcgcgt cgagcgcggc gaagggctcg tcgagcagca gcaccggccg
    13261 tcccgccagc agcgtgcgga cgaaggcgac ccgttggcgc atgccgcctg acagtgcggt
    13321 ggggcgtgcc cgctcgaagc cggcgaggcc gaagcgctcg aacagcggca tggccctcgc
    13381 acgtgcggcg cgtgctgcaa ccccgcgcgc ctcgagcgcg aggccggcgt tgcccagcgc
    13441 gtcccgccac ggcagcagta gatcgcgctg cggcatcagc gcgcaggcgt cgcgacgctc
    13501 gcgggcgtca tcgcgctccc cgacgctgac ccttccggcg tcgggctcct gcaggccggc
    13561 gacgagctcg agcagcgtcg atttgccgca gccagaggga ccgacgacgc caagcacctc
    13621 gccgcccgtc agcgagagct cgaggtccgc gagggcggtc agcgacgcgc cacgccgggt
    13681 ggcgtagcgg cgcgtgacgc cggtcaacgt cacgccggtt gcctcgacgg agagaggcgc
    13741 cctcggcggg gcctcgacct tcatgcggaa tctgctccct tcgcgggcat taccccacag
    13801 gttcaaaggg tcagcgccag gcgagagaac tcccgggggc gctatctcag cccgcttccc
    13861 gcgagccccc ctgtcgtttc cgggcactct agcgccgcgg cgccggagca gtccgaaggg
    13921 cgacgtggcg caccggcgag gagggcggtg cccctaggca caccgccctc ccgctagccg
    13981 gtcccgatac ccagcggtat ggggacgatc ccggttggat gggcctggga taccgcatcg
    14041 cccaccaccc gattgagatg accgtaatac ccccgcacgc ggctgtccat acctgcgcga
    14101 gagtactctc gcgcaggtat gccagctgtc cgacggctca atcggagcgc tgcgaatagg
    14161 cgattcgcca gaggcggagc agtggcagca gcacgcgaac caaggagccg cccgcacgga
    14221 ccagggtgcg cgtcgagcgg cgcaggtcgg atgcccgggc actgtccttc gacaggtaca
    14281 tcgcgaccat cgctccgact atgacggcat ggcggttccc gttcggcagc tgtagggcat
    14341 cgcgcgccgc gtgcgcgatg cagtgacaga gccgccgtac cgcacagtca tggtccggat
    14401 aatgcgagat gtaactgtga tcgcctgacg cacgatcctc cgcctgatcg acgtagctgt
    14461 cgagcatcgt cgtcgcgagc gagatccaag gccagtagac ggcatagatc gccgaaatgt
    14521 cccgatcggt ggcgttcgcc tccgccgcga tcgccagcaa ggcgtgtacg accatcgatg
    14581 cgcttgccgc gccgctcagc tcgaaccacg tcgcaccata agcgccggga cactcgaccg
    14641 cagcccagcg ccgtagcgcg gcgtcacggt tctggtcgtc ggtcaaatgg ttgagcgcga
    14701 gcacctgggc ccgccacgac tcctgtatca acagaagacg tacccggcca tacgagggga
    14761 gttcctcaca gcttcggcga cagaattcga ccagcgccct caggtagccg ccgtcgttct
    14821 cccatgggtg gtgacggtag tagtcggcaa gggggcgttc cggatccaac gcgtcgacga
    14881 gcgcgaggtg gagttcacgg ccgttagcct cggtggtgtg acgctcgctg acattgtcga
    14941 gaaagtccca gatcgtctcg tacgcgacca gcaggtggag cagcaccgtg ttgcggcgct
    15001 gtggcagtgc gctgaacagc gcggcgccgt ctgcgtggcc gcgcttgtgt gtcagcgaat
    15061 agagcgcatc ctcgcggatc ggctcgtcgg ggatggtctc ggcgagcgcc cgccagcggc
    15121 gcgtcgcccg ggcggcctcc ggcagccccc atacgagctc gcgtacagcg gcggcgaaca
    15181 gcgcccgaac ttgcccagag gtaagcggcg tcggatcgac atgagcggct tgcggcatcc
    15241 caaggccagc ctattgccat taggaccagg tccatagcga atttttcgct ctatccctgt
    15301 tgcgttgggc accaccgatg gagtacggtc gtgtccgtat tggaggttga aggcggaggg
    15361 gatggcgaag ctctcgacga agcatgtcgt gctgggcctc atgatcgagc ggcccggcta
    15421 cggatacggc ttgcagcagc agttcagcga tcgcttgggt tttctcggtc tcgccgagag
    15481 ctccgtctac aagaccatcg aacggctcga ggccgatggc tgggtcgagg cgatgggatc
    15541 gcggcggccc gacagtcacc accgcgccac ccagcgcgtc ctgtaccgcg ccacgcccga
    15601 gggtgtcgtg cagttcaagc gctggatggc gcagccgagc gatcgcgcaa tcgtccgtga
    15661 cgagctacac gcaaagctgg cgctctccaa cccgacggac ctccccgacc tgttgcgtac
    15721 ggccgaggcc caatcgcgcg agtgcctcgc cgagctcgcg gcgatcggag gacgatcaat
    15781 ccccgtgagt gccgacctgc cctggggcat ggtcgcgacc gcgctcgtcg acgacttcaa
    15841 ggcgcgctgg ctggaggcgc tcgtcgactg gttggaccag atgtgcaggg tgatcgagga
    15901 gcggatggcg cagggcgctg tagacgcgag ctgatctcgg catgccgctg ttgcaactcg
    15961 agtcggccaa caggtcgttc tggcgcgggt cgaccgagat tcgcgtgctg cgcgacgtca
    16021 cgctgagcgt cgatcaaggc gattttgtcg ccgtctacgg gcagcgcagc gcgggcaaga
    16081 cgaccctgct caagaccgcg gcgggcttcg agccgctgga tgccggaagc gtcacctttc
    16141 gtggtgagga cctggcagcg atgtcgcggc gcaggctgac gcggttgcat cgcgacgaga
    16201 tcgcctgggt cgagcacgcg ggaccgcgca gctttgggct gtcgatgcgt gcctatgtgg
    16261 ccctgccgct gctcgggcag atgggcgcca tgcaggcgca gcgtcgtgcg gcgtccatgc
    16321 tcgaggaagt gggcgtcgac gattccgggg atcgacgctg ggacgagctc tccgatgcgg
    16381 cgaggatgct cgtcgcgatc gcgcacgcgc tggtgcgcaa gccggacctg ctgatcgtcg
    16441 acgacccgac agtcggtctc ggcatggcgg atcgcgacgc gatcgtcgcg ctgttgcgcc
    16501 acgccgcgga cgacggtgtg gcgattttga tggcggtgcc cgatatgccg gccatggtgc
    16561 acgccgacca ggtgcattcc ctgagtcgcg gccggctgct ctcggttccg accgccgcac
    16621 gtggcagcca cggcaacgtc ctggagttcc cgggtcgaac gggaagctcg tgatgctgga
    16681 gctgcgggac ctggtcaagc actatcgatc gggccgcgac gagacggtca gggcgctcga
    16741 cggcgtgtct atgacgattc aggcgggcga gttggtggcg ctctacggcc cgagtgggtc
    16801 cggcaagacg accttgctat cgatcgtggc ggcgttgttg ccacccgacc gcgggaccgt
    16861 gaggctggat ggccgggagt tgtcgtcgct gtcgcccaag gcggcggctc actacaggat
    16921 gcacgacctg ggatacgtca gccaggcgcc ggacctgttg cccggcgtac cggcgatcga
    16981 caacgccgcg ctgaagcttt tcggcatgcg aatgggggtg cgcgaggcac atcgccgcgc
    17041 gatcccgttg atggagcggt tagggcttgg ggccaggctc gggcaccgtc ccgatcagct
    17101 gtcggctgga gagcgccagc gagtcctgat cgctcgcgcg ctgtcgaccg ggccgagcct
    17161 cgtgctcgcc gatgagccga ccggaagcct cgacaccacg cgcagtcgcg aggtcctgac
    17221 cctgctcacg gagatctgtc acgagcagcg gaccgcgatg ctgctggtca cccacgaccc
    17281 gcgcgccgcc tcgttcgcca gtcgtgtgca cgcgctggtc gacgggcagg tggtcgccta
    17341 cgagccggat gacctcggcc tgccggcgcc tgtccctcag gcctaggccg atgcgcctct
    17401 ccaacacgct cttcctttat cgcacccggc tgcgttcccg gctggtacag gagctgctgg
    17461 cgctggccgg gatcacgatc ggcgtggcgc tcgtattcgc ggccctggtg gcgaacagca
    17521 gcctcaccgg atcggtcagg cagctgaccg agggaatcgt cggcgaggct gacttccagc
    17581 tggtggcacg gggtccgatc ggcatcgacg agcggctggt cggcgaggtt aggcggctag
    17641 tgggcgtcga ggccgcggct cccgtgctcg aggctcgtgc caacgtgatc ggccccgacg
    17701 gacgacgctc gatccagctg ttcggcggcg atccgcgctt cgtcgagata ggcgggccgc
    17761 tgctggaggt cttcgccacc gacagcccgc aggaggcgcg ggtactgagc ggtcagcagg
    17821 gactggcgat tccggagccg atgaccaagg ccctgggcat cctgcctgcc cacccattcc
    17881 aactggagat cggcagtagg agggtccatg tccccgtcgc gttgtcgctg ccgcgcgatg
    17941 cgatcggccc tctggtcgac agtcccgtgg cgatcgcccc gctcgcctat gcccaggaga
    18001 tcgccgatct caaggggcga gtgagcagaa tcttcgtcaa gccggtggcg ggacgggagc
    18061 ggcaggtcga gcgggccttg gaacgagtcg ccgccggccg agccaacgtg cgtgacgccg
    18121 acgccgacgt cgcgatcttt gagaacgccg cctatccgac gaaccagtcg acaaccctgt
    18181 tctcggtctt cagcgcgctg gtcggcttcc tcttcgcatt cagcgcgatg ctcctgacgg
    18241 cgcagcaacg ccgcggccta gtcgccgaca tgcggatcgc gggccacacg acgtggtcgt
    18301 gcgtacaggt tctgctcttc gacgcactcg tgctcggcgt cctcggctcc acgctcggga
    18361 tcgcgctcgg tgaccaggtc tcgcggcatc tgttcgacgc cgtgccgggc tatctcgcgt
    18421 tcgccttccc actcggagat cagcgcgtgg tcacctggca gagcgtcgcc atcgcgggcg
    18481 gggccggggt gctggcggcg tgcttcgctc tgcttgcgcc tctgcgtgac atgaccttgg
    18541 agcacccgct ggtcagtcgg tcgcggctga aactgcagcg cacccagagg gcgctggcgc
    18601 tcgcggggtt ggcggccctg ttcgcgacga cggtgatcct cgtcgcgtac cccgacgccg
    18661 ccatcgccgg gatcgtgagc ctgaccttgg cgctcctcct gctgctgcca ctgcttctgc
    18721 gttcgacaat gctggtgctg gcgctggtgg tgaggggcat gaagagcgtc gtcccgaccc
    18781 tcgcgatcct cgagctgcgc tcgcgctctg ctcaaactcg gacgctagcg gttgccgcga
    18841 ccggtgcgat cgccgtcttc gcgacggtct cgatcggcgg cgcccacgcc gacctgcagc
    18901 gcgggctgga tgcgtcggca cgtgagattg acggcgtggc cgacatctgg gcgacctttc
    18961 ccgggacccc gaacgccttt gccacgaccc cgttcgaggc cacgccggcg atgctggacg
    19021 caaccgagcg cctacccggt gtacgagcgg ttcgcaccta tcgcggcggc tttctcgacc
    19081 tgggcaatcg acgtgcgtgg gtgctcgcac caccagcggc cgtcgccatg ccggttccgc
    19141 ccagccagat cgagaagggt gagctcgacg agatagccgc ccgcgtgcga tccggcggct
    19201 gggtgacggt ctccgaggcg atcgcgaagg actacgacgt aggggtcggt gactcgatcg
    19261 tcctgcccac gccacgccct accccgacgc gcgtcgccgc ggtcacgacc aacctcggct
    19321 ggccgcccgg agcggtcgtg ctcaatgcag acgactatgc aaaggcgtgg ggaagtgagg
    19381 ccgcgagcgc ggtgcacatc gacgtggcgc cgggcgcctc gccggaccgc gtagcggggg
    19441 ttgtgcgcca gacactcgga gcggagctgc cgttgcgggt ggagacgcgt gccgagcgcg
    19501 tcgatcgcca ctttgccgct gcgcgccaag gcctttcgcg cctgacgcag atatcggccc
    19561 tcgtgctgat ctccgccatc ctcgccatgg cgattgcgat ggcgggcatg atctggcaac
    19621 gcagacccac ggtggccaag ctgaaggttc acgggtactc cgagcgcgag ctgtggggag
    19681 ctttgatggt ggagagcgga gtcctgctcg gtgcgggatg cgtcgccggt gcgctgttcg
    19741 ggctctacgg gcagttgctg ctgagccgcg cgctgcagac gatcaccggc tttcccgtga
    19801 cctacagcgc ggctggcctg accgcgctgg cgatcctcgc cctggtgacc gccgtggcgc
    19861 tcgcgatgct cgcgctgccg ggctggctcg cggtgcgcat caggcccacc ccgggcttga
    19921 cgtcctgatc ggccggggga aaggcggcta acggctcgtg accggacgca ggtcggcgat
    19981 cgcgttgacg aagccctcga gctgcttggt tgtccagcac tcgaagacgc cgttgcagaa
    20041 cggctcgtag acctgcatca ccgagtcacc gtagttccag tagagcttcg gctccgggtt
    20101 catccagaat gtcctcccgg catggtcggc gatctggccg aaggcagcgg cgtggggctc
    20161 gcggccgttg gtccttgcgt cgccgagcac gatcaccgtc gagcgcggat cgaggtcgtc
    20221 gaccacgcgg tcgaggaact cgatccacac gcgcccgtaa tcggtgtagc ccgagacgtc
    20281 agcgacgccg gcgtcgccgg agatggcacg cgagacggcc tcgaaggagc gctcgcgctc
    20341 gaagacgtcg gtcacctcgg agatccgctc gacgaacacg aacgagcgca gcttgcgaaa
    20401 cgagtcgtgc agcgcgtgca ggaccgatag gaagaacacg ctcgcgctcg tgacgctcgt
    20461 cgacacgtcg cagagcacgt agagctccgg tctgcgcggc cgccgcgggc ggaagcgcaa
    20521 atcgagcggc acgccgccgg tctgcagtga cgtgcgcatc gtgcggcgga cgtcgatcgc
    20581 ggacgagcgg ctgcgtccgc gcgtctcacg gccgtgggtg gcgagccggc gacgcagctg
    20641 ggcgacgacg cggtggacct gcgcgaggtc ctgggctgcg ccggtcggca atgcccgatc
    20701 gagctcgcgc aggggcttgc tcggcggaag cgtctgggtc cgctcgatca gctggcgctc
    20761 gagctcgcgc cgtacctggg cctcgaactc gcgcagccgc tcgcgtggga ccgcctcggg
    20821 gtcgggcagc tcggcgcccg cctgctcacc gcgcaggcca agcgtgcgac ggattcgctg
    20881 gacgtcgacg ccgatcacgc ccgagccctc gccctggcga ccgaacgccg agatcgccag
    20941 ccgggccagg tcgcgcagtt gaccctccgg cgccgcggca tcctgcagcg cgtcacggat
    21001 tcgctcgcgg agcgactcga agtcgagctc cggctcgcgg gtgccgccac cctcgcgcgc
    21061 gggcgcctct ttaatccccc gctcgacggc ctcgcgctcg gcggcgcgga agaagaagcg
    21121 gtcgaagacg acctcgagca cacgccggtc gtcctgggac ttcgcgagcg tggtcgcgag
    21181 cgcctcgaag aagtcgcgcc ggtcggtcca gtcgacatgg cgcagcgcct cgaaggcgtc
    21241 gagcagctcc gaggtcccga ccgccatctc ctcgcgccgc agctcgtctg cgagctcgag
    21301 cagccgcggc gccatcccgg ctggaccggc cgaagccggc atgccgccgt agaggcttcg
    21361 cctacccggc cgggcgggat ccaacgagtc cgccatcgcc cagcttcacg ccgacacgct
    21421 cggcgacgag gtcgagatcg ctgcggtgct tgacgattac gctcatcgtc tgcttcagcg
    21481 tcgtctcgtc gacatcctcg gcgccgagca gcagcagtgc gcgtgcccag tcgatcgact
    21541 cggcgatcga aggcggcttc ttgaggtcga ggtcgcgcac catgtgcatc acctcgacca
    21601 gcttgcggcc gacggcctcg ctcagatcgg gtgcgtgcag gcggacgatg ccgagctcgc
    21661 gctcgactgt cggatagccg agccagaggt acaggcagcg gcgcttcagg gcctcggtca
    21721 gctcgcgcga gtcgttcgag gtcagcagga cgatcgggtg ggtagatgcc tcgaccaccc
    21781 cgagctcggg gatcgacacc tggaagtcgg acaggacctc gagcagcatc gcctcgaact
    21841 cctggtcggt cttgtcgatc tcgtcgatca gcaggacgac cggctcagcc gccgagatcg
    21901 ccgtcagcag cggccgcgga agcaggaact cctcgccgaa gatgtcgtcc ttgacctcct
    21961 gccagccggc gtcggaggcc tcgacctgga tccgcagcag ctgcttgcgg tagttccact
    22021 cgtagagcgc cttcgcctcg tcgagcccct cgtagcactg cagccgcacc agcttgcgcc
    22081 cggtcgcccg cgagagggcc ttggcgagct cggtcttgcc gacgccggcg ggcccctcga
    22141 cgagcaccgg cttgcccagc cgcccggcca ggaacgacac gagcgccgtc gcctcgtcgg
    22201 cgaggtagcc ggcctccccc agccgcacgc gcgtctgctc gatcgactgg agctccgccg
    22261 tcaccccgac aaggatactt tcggtcggtg tccggcctcg atcccctcct cctgctggcg
    22321 cagttcctcg atccgagccg cgggcccgac cagaccggcg acttcaaggt ctcgccgcgc
    22381 cccttcctcg ccaccatcgg cgccgggttc ctgatcggga cgatcggcca cgtgctcagg
    22441 tcgaggacgc tcgtcgcctt cggggtagcg ctgatcttcg gcgccacggt ggcgctcccg
    22501 ctggcgctcg cgatcgccaa ctagcccccg cgaggcgcct gccggagccg tccgttggct
    22561 aatctcggac gttcgtccca gcc
//
LOCUS       DATABV010000153         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2136330, whole genome shotgun sequence.
ACCESSION   DATABV010000153 DATABV010000000
VERSION     DATABV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136330"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VHF88_06775"
     CDS             complement(<1..399)
                     /locus_tag="VHF88_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2161507.1"
                     /translation="MHTQSAPKIVGPSDGKAGNLGSIGVRFMIDTEETHAGGFSLVEH
                     PLPPRRLAAPLHRHSREDEYSYVVEGRMGALLRDDVVYAEVGDLVHKPRDQWHTFWNA
                     GDEPCRILEIIAPGGFEDFFDELVDANLAGA"
     gene            466..1080
                     /locus_tag="VHF88_06780"
     CDS             466..1080
                     /locus_tag="VHF88_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161508.1"
                     /translation="MSTQKPKRRYELKARAEAQEATRARIAAAAAELHGEVGVAKTTV
                     SDIARRAGVQRLTVYNHFPNLETLLPACTAHWLAEHPMPDLEAALAIEEPGERVRAVL
                     TLLYGWYRRTAPMQRRVCGERSTVPELDAWMAQSSDQLLAGLAERLAEGVDSPGARPL
                     ICVALDFWTWQRLDGEGLDDAAAADLMAGVACRAGGAPTATSTD"
     gene            1108..2190
                     /locus_tag="VHF88_06785"
     CDS             1108..2190
                     /locus_tag="VHF88_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161509.1"
                     /translation="MSTASAARSESLWPRLAELPLVVEGCEYDRLHAVLANDFDRVTT
                     HVRLVGGGTEGLGEDISAHEEDGTTLHETRPSLPLEGEWTLSAFSEHLATLELWSQPP
                     EWEAAPRYRAWAFESAALDLALRQTGRSLHDVLGLEPQPVRFVNSLGLGEQPSIEPLR
                     RRLARSPGVRFKLDAEATWSPALVDEVAATGAVDTIDFKGHYGFEVEDPDALGALYDR
                     VLDAFPDAYLEDPHDLPEIAQRLGDHLDRVSYDAPIHSADDIGATPLAARVVNVKPSR
                     IGSLRLLFEVYARCAREGRPMYGGGMGELGVGRGQIELLAALFHPDAPNDVAPSAYNE
                     DDPAGELPASPLAPRPETTGFRWVTA"
     gene            complement(2210..>2951)
                     /locus_tag="VHF88_06790"
     CDS             complement(2210..>2951)
                     /locus_tag="VHF88_06790"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX2161510.1"
                     /translation="ERNPAERPFPFMPTLDPAHYLAEHLAAWPAPVVVYRAVSKYAPA
                     ELRSWLSAQDPTRIMTVLVGAPSSATAGRTSLADAQALHRDTNPALSLGGVAIPERHT
                     RREDEHLRLLAKQEAGCRFFVTQVVYDINAAKNLVSDYHYECQARGVAPVPFVFTFSV
                     CGSMKTLEFLRWLGVDVPRWIENDLAHATDPLEASYEQALTTATELMAYCRTLGVPLG
                     INVESVSIRRVEIDASVRLAEQLRAQLH"
BASE COUNT          432 a         1012 c         1080 g          427 t
ORIGIN      
        1 cgcgcccgcg aggttcgcgt cgacgagctc gtcgaagaag tcctcgaagc cgccgggggc
       61 gatgatctcg aggatccggc agggctcgtc gccggcgttc cagaacgtgt gccactggtc
      121 gcgcggcttg tggacgaggt cgccgacctc cgcgtagacc acgtcgtcgc ggagcagcgc
      181 gcccatccgg ccctcgacca cgtaggagta ctcgtcctcg cggctgtggc ggtgcagcgg
      241 agcggcgagc ctgcgcggcg gcagtgggtg ctcgacgagc gagaagccgc cggcgtgggt
      301 ctcctcggtg tcgatcatga agcggacccc gatcgagccg aggttgccgg ccttgccgtc
      361 gctcgggccg acgatcttgg gggcggactg ggtatgcatg acgaagctcc tttcgatgaa
      421 cacagcgttt agtgattgac cagcactgta acatgggtga acgccatgtc aactcaaaag
      481 cccaagcgcc gctatgagct gaaggcccgg gccgaggccc aggaggcgac ccgcgcccgc
      541 atcgccgcgg cggccgccga gctgcacggc gaggtcggcg tcgcaaagac caccgtctcg
      601 gacatcgccc gccgcgccgg cgtccagcgc ctgaccgttt acaaccactt cccgaacctc
      661 gagacgctgc tgccggcgtg caccgcccac tggctggccg agcacccgat gcccgacctg
      721 gaggcggcgt tggcgatcga ggagccgggg gagcgggtgc gagcggtgct gaccctgctc
      781 tacggctggt accgccgcac cgcgccgatg cagcggcggg tgtgcggcga gcggtcgacg
      841 gtcccggagc tggatgcgtg gatggcgcag tcgagcgacc agctgctggc cgggctcgcc
      901 gagcgcctgg cggagggcgt cgacagtccc ggcgcacgtc ctctgatctg cgtcgcgctc
      961 gacttttgga cctggcagcg ccttgacggc gagggcctcg acgacgccgc ggccgccgac
     1021 ctcatggcgg gcgtggcctg ccgcgccggt ggtgcgccga ccgcgacctc tacggactga
     1081 caagcgccac gcgcaatgat ggccaccatg agcacagcga gcgcagcgag gagcgagtcc
     1141 ctctggcccc ggctggccga gctgccgctg gtcgtcgagg ggtgcgagta cgaccgtctg
     1201 cacgccgtcc tggccaacga cttcgatcgc gtcacgacgc acgtccggct cgtgggtggt
     1261 ggtaccgagg gactcggcga ggacatctcg gcgcacgagg aggacggcac gacgctgcac
     1321 gagacccgtc cctcgctgcc gctggagggc gagtggacgc tgtccgcctt ctcagagcac
     1381 ctcgccacgc tcgagctgtg gtcgcagccg ccggagtggg aggcggcgcc gcgctaccgg
     1441 gcctgggcgt tcgagtcggc ggcgctcgac cttgcgctgc gccaaaccgg ccgctcgctg
     1501 cacgatgtcc tcgggctcga gccgcagccg gtgcgcttcg tcaactcgct cggcctcggc
     1561 gagcagccct cgatcgagcc gctccgccga cggctcgctc gctccccggg cgtgcgcttc
     1621 aagctcgacg cggaggcgac ctggtcgccg gcgctcgtcg acgaggtcgc ggcgaccggc
     1681 gcagtcgaca cgatcgactt caagggtcac tacggcttcg aggttgagga cccggacgcc
     1741 ctcggcgccc tgtacgaccg ggtgctagac gccttccccg acgcctacct ggaggacccg
     1801 cacgacctgc ccgagatcgc gcagcgcctc ggcgaccacc tcgaccgcgt gtcctacgac
     1861 gcgccgatcc acagcgccga cgacatcggc gccacgccgc tggccgcacg ggtcgtcaac
     1921 gtgaagccct cgcggatcgg gagcctgcgc ctgctgttcg aggtctacgc gcgctgtgcc
     1981 cgcgagggcc ggccgatgta cggcggcggc atgggcgagc tcggcgtggg gcgcggccag
     2041 atcgagctcc tcgccgccct gttccacccc gacgccccga acgacgtagc gccgagcgcc
     2101 tacaacgagg acgacccggc gggggagctg ccggcaagtc cactggcgcc gcgcccggag
     2161 acgacgggct ttcgctgggt taccgcctag tccggcgcag cagagagtgc tagtgcagct
     2221 gcgctcgcag ctgctcggcg aggcgcaccg acgcgtcgat ctcgacccgg cgaatcgaca
     2281 cgctctcgac gttgatcccg agcggaacac cgagcgtgcg gcagtaggcc atcagctcgg
     2341 tggctgtggt gagcgcctgc tcgtaggagg cttcgagtgg atcggtggcg tgggcgaggt
     2401 cgttctcgat ccagcgcggg acgtcgacgc ccagccagcg caggaactcc agggtcttca
     2461 tcgacccgca gaccgagaac gtgaagacga acggcacggg cgccacgccg cgcgcttggc
     2521 actcgtagtg gtagtcggag acgaggttct tggcggcgtt gatgtcgtag acgacctggg
     2581 tcacgaagaa tcggcagccc gcctcctgtt tggcgagcag gcgcaggtgt tcgtcctcgc
     2641 gccgggtgtg gcgctcgggg atggcgactc cgcccagcga cagggccggg ttggtgtcgc
     2701 gatgcagggc ttgggcatcg gcgagtgatg ttcgaccggc ggtggcgctc gacggcgcgc
     2761 cgaccagcac ggtcatgatc cgtgttgggt cttgggccga cagccacgaa cgaagctcag
     2821 cgggcgcgta cttgctgacc gcgcggtaga cgacgaccgg cgccggccag gccgccagat
     2881 gctcggcgag gtagtgggcc gggtccagcg tcggcatgaa cgggaacggg cgctcggccg
     2941 ggttgcgctc g
//
LOCUS       DATABV010000154         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_369649, whole genome shotgun sequence.
ACCESSION   DATABV010000154 DATABV010000000
VERSION     DATABV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369649"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VHF88_06795"
     CDS             complement(<1..147)
                     /locus_tag="VHF88_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2161511.1"
                     /translation="MAGLDAVTDNNFQAEVVEADKPVLVDFWAPWCGPCRQVSPVVEQ
                     LGEEL"
     gene            365..1393
                     /locus_tag="VHF88_06800"
     CDS             365..1393
                     /locus_tag="VHF88_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161512.1"
                     /translation="MGTALTMVALAAPAAVADVEPNGAVFTPEGPIFGGQDIQGAVGP
                     GDTDDWYVFHVEGAHQLHLRSPHIALSPGGGQGPTSTACVSVQLTNANGMAIPSDFTS
                     GPGESTFYVHAFQSGFKGCSIHAEYSFRVDPAGALVPGPGKLPIKGTAEANDSRSTAG
                     GPLIAGAWYHSELETANDRDWLRFYVRPGTRRIDVRSVAYGSPCDTHEVTLRSARGSE
                     LASYTGTSGTIAHFVHRPRGGARLYVEIVNGAPSLAAAEACVRSATVVQVGPEEAIMS
                     AAEVKRGCVDGRKAARRGARRVAIVKRAIVRAQARGAATGQLRRALRREQRKLRRSRG
                     LVGAYCSR"
     gene            complement(1430..2149)
                     /locus_tag="VHF88_06805"
     CDS             complement(1430..2149)
                     /locus_tag="VHF88_06805"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX2161513.1"
                     /translation="MAGVWLILPTYNEAENIEAIVTAALPQLASTGLDHHVLVVDDGS
                     PDGTGAIADRLAAADPRVEVLHRTSKDGIGPAYLAGFAHALARGADLLMEMDSDFSHD
                     PSDIPRLVAAAERADLVLGSRYVPGGGVTDWGLGRRLISRGGSLYAKLLLSIPVDDLT
                     GGFKCFRRTVLDTLDLSGVGTDGYGFQIEVTYRAIKAGFRVEEVPILFRDRRVGSSKM
                     SWRIAVEAFWKVPLLRWRVRA"
     gene            2308..3087
                     /locus_tag="VHF88_06810"
     CDS             2308..3087
                     /locus_tag="VHF88_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2161514.1"
                     /translation="MTGELFSVKGKTAVVTGGSRGIGLMIARGLLEAGAEVVISSRKA
                     DQLDAAAAELSEIGKCIAVPADLSSQEGAIALADAVRERFDRLDILVNNAGASWGEPI
                     DTYPDKAWDRVLDTNVRGVFQLTVASLDLLRESASADDPARVINIGSVDGLRPPMMES
                     YAYSASKAAVHQLTRHLAKRLAAEQITVNAIAPGPFPSKMMAFVLDDPDARAVVEQTI
                     PLGRIGEPDDVAGLTIFLASRAGAYLTGTVIPLDGGYSGAS"
     gene            3182..>3489
                     /locus_tag="VHF88_06815"
     CDS             3182..>3489
                     /locus_tag="VHF88_06815"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161515.1"
                     /translation="MKNAEIAAAFDELGTLYELDGAVVYRVVAYRNAAKAIRDSGVSV
                     AELARQGRAEELPGIGKTIAEKIDALLLDGEIPAAVKLKERIPPGLIKVTRIPGLGPK
                     R"
BASE COUNT          533 a         1184 c         1280 g          492 t
ORIGIN      
        1 gagctcctcg ccgagttgct cgacgaccgg gctcacctgg cggcaggggc cgcaccacgg
       61 tgcccagaag tcgaccagca ccggcttgtc ggcctccacc acctcggcct ggaagttgtt
      121 gtcggtcact gcgtcgagac cggccattcg ggctccttca gcgcgtcggg atcgggttca
      181 atgcttcaaa aagtatagcg aggccgtggt gcagcccacg gccgctcccc ggagcctacc
      241 cgcgacttcc gtcgtctacc cgggttggaa tactcggcaa cccgcgcgcg cacgcggcgg
      301 ggacaaatcg gtcgacagcg ggggaactgc cgtgcacacg aagacgcaag cggcggcggg
      361 gatcgtggga accgcgctga cgatggtcgc gctggcagcg ccggcggcgg tcgccgacgt
      421 cgagccgaac ggggcggtct tcacgcccga gggcccgatc ttcggtgggc aggacatcca
      481 gggggccgtt ggcccaggcg acactgacga ctggtacgtc ttccatgtcg agggcgccca
      541 ccagctccac ctgcggtcgc cccacatcgc gctcagtccc ggcgggggtc aggggccgac
      601 ctcgacggcg tgtgtctcgg tacagctgac caacgcgaac gggatggcga ttccctccga
      661 cttcacgagc ggccccggcg agtcgacgtt ctacgtccac gccttccagt ccgggttcaa
      721 gggctgttcg atccacgctg agtacagctt ccgggtcgac ccggcggggg cgctggtccc
      781 aggaccgggc aagctgccga tcaaggggac ggcggaggcc aacgacagtc gctccacggc
      841 gggtggcccc ctgatcgccg gtgcttggta ccactcggag ctcgagacgg cgaacgaccg
      901 cgattggttg cgcttctacg tccggccggg cacccgtcgc atcgacgtgc gttcggtggc
      961 ctatgggtcg ccctgtgaca cgcacgaggt aacgctgagg agcgctcgcg ggagtgagct
     1021 cgcctcgtac acggggacgt ccgggacgat cgctcacttc gtccaccggc cgcgtggcgg
     1081 tgcgcggctg tacgtcgaga tcgtcaacgg cgcgccgtcg ctcgccgccg ccgaggcctg
     1141 cgtgcggtcg gcgaccgtgg tccaggtcgg gcccgaggag gcgatcatgt cggccgccga
     1201 ggtgaagcgc ggctgcgtgg acggtcgcaa ggcagcccgg cgcggcgcac gccgggtggc
     1261 catcgtcaag cgggcgatcg tcagggcgca ggcgcgcggc gccgccactg gccagctgcg
     1321 ccgcgcgctg cggcgcgagc aacgcaagct gcggcggtcg cgcgggctgg tcggcgcgta
     1381 ctgctcgcgc tgaccggccg gccactcatg gcgcctgatg gcgggtcgcc taggcgcgga
     1441 cccgccagcg cagcagcggc accttccaga acgcctcgac ggctatccgc cacgacatct
     1501 tcgacgagcc gacgcggcgg tcgcggaaga ggatcggcac ctcctcgacg cggaagcccg
     1561 ccttgatcgc gcggtatgtg acctcgatct ggaagccgta gccgtcggtg ccgacgcccg
     1621 agaggtcgag cgtgtcgagc acggtgcggc ggaagcactt gaagccgccg gtcaggtcgt
     1681 cgaccggaat gctgagcagc agcttggcgt agagcgagcc gccgcggctg atcaggcggc
     1741 ggccgagtcc ccagtcggtc accccgcctc cggggacgta gcgcgagccg agcacgaggt
     1801 cggcgcgctc ggcggcggcg acgaggcgcg ggatgtcgga ggggtcgtgc gagaagtcgg
     1861 agtccatctc catcagcagg tccgcgccgc gcgcgagcgc gtgggcgaag ccggcgaggt
     1921 aggcggggcc gatgccgtcc ttggacgtgc ggtgaaggac ctcgactcgc gggtccgcgg
     1981 cggcgaggcg gtcggcgatc gcgccggtcc cgtcgggcga gccgtcgtcg acgacgagca
     2041 cgtggtgatc gagcccggtc gacgcgagct gcggcagcgc cgccgtcacg atcgcctcga
     2101 tgttctcggc ctcgttgtag gtcgggagga tgagccagac gcccgccata ggcgcgacac
     2161 cctatgtgcc gcgcccgatg atggcttgtg ccgtctaggc gatgcaaacc cctcatcggc
     2221 gggccgggca gccacccaat cgcgccggat gatggcttgt ccgtctaggc gatgcgaatc
     2281 cctcatcggc gcgataggtt ggcgcgcgtg acgggggagc tgttctcggt gaaggggaag
     2341 accgcggtcg tgaccggcgg gtcgcgcggg atcgggctga tgatcgcgcg cgggctgctg
     2401 gaggcggggg ccgaggtcgt gatctcctcg cggaaggcgg atcagctcga cgccgcggcg
     2461 gccgagctgt cggagatcgg caagtgcatc gcggtcccgg ccgacctgtc atcccaagag
     2521 ggagccatcg ctctcgccga cgccgtgcgc gagcggttcg accggctcga catactcgtc
     2581 aacaacgccg gcgcgagctg gggcgagccg atcgacacct accccgacaa ggcgtgggac
     2641 cgcgtgctcg acaccaacgt ccgcggcgtc ttccagctga cggtcgcatc gctcgacctg
     2701 ctgcgtgaga gcgcctcagc cgacgacccg gcgcgcgtga tcaacatcgg ctcggtcgac
     2761 ggactgcgcc cgccgatgat ggagtcctac gcctacagcg ccagcaaggc cgcggtccat
     2821 caactgaccc gccacctcgc gaagcggctc gccgccgagc agatcacggt gaacgcgatc
     2881 gcgcccggac cgttcccgag caagatgatg gccttcgtcc tcgacgaccc ggatgcgcgc
     2941 gcggtcgtcg agcagacgat tccactcggg cggatcggtg agcctgacga cgtggccggc
     3001 ctgacgatct tcctcgcatc gcgcgccggc gcctacctca ccggcaccgt catcccgctc
     3061 gacggcggct acagcggcgc gagctgaccg cgggcgggct ccccggccgc tcgatgaagg
     3121 attcgcctcg cctggacgcc acaatcgttc atcgagcacc tcgagccgct agaaagagac
     3181 gatgaagaac gcggagatcg cggcggcctt cgacgagctc gggacgctct acgagctcga
     3241 tggcgcggtc gtctaccggg tggtcgccta ccggaacgcg gcgaaggcga tccgcgactc
     3301 gggcgtctcg gtcgcagagc ttgcgcggca ggggcgggcg gaggagctgc cggggatcgg
     3361 caagacgatc gccgagaaga tcgacgcgct gctgctcgac ggcgagatcc cggccgcggt
     3421 caagctcaag gagcggatac cgccggggct gatcaaggtc acccggatcc cgggtctcgg
     3481 gccgaagcg
//
LOCUS       DATABV010000155         8544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_290703, whole genome shotgun sequence.
ACCESSION   DATABV010000155 DATABV010000000
VERSION     DATABV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290703"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VHF88_06820"
     CDS             <1..672
                     /locus_tag="VHF88_06820"
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2161516.1"
                     /translation="DAGAEAGDTGAAGSLLALASAWQGAERGDRCAALAYESGAGADA
                     LLVEATAPPPPTATQPARRPVGYVELLRRRDLLEPPPPPDPSAPYAATPAALRDAPYV
                     DDLTGLECAVCGSLNFPARPTCIDCGSSELSPVPMPRTGRVVTHNEQFVVGVAPEPAP
                     VSVGVVRLDGASGERGGQVSAMFVDGDAAIEIGAPAELVHRRLGSERGLVKYGWKLRI
                     VGEGA"
     gene            669..1847
                     /locus_tag="VHF88_06825"
     CDS             669..1847
                     /locus_tag="VHF88_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX2161517.1"
                     /translation="MSRDLGVSVVAAGMTRFGERFDASYEELAADAYGACLESSDGRI
                     DGERLEAAWLSTALGGLIRREAVTGASLADPLRFFPRPVTRVENACAGGSDAIRNAAF
                     SVAAGVYDIVLVLGVEKMRDIPTRASLIAQRGQMSHLWWHPRGASSPYLFAQHATVHM
                     ERNGLTREHLARVAAKNRRHGALNPKAFMRREIEVEDVLDAPMVCSPLGLLDCCPTTD
                     GAAAVVLARSDLAAELTDAPVELAGLGLATDVPYSHWKPSFEYQPAPTRAAEIAFEMA
                     GIAPGDVDVAELHDCFSVAEVLAYEDLGFCGRGEGGRWLDSGAPELGGELPCNTSGGL
                     LAKGHPIGATGIAQMAEVFWQLRGEAGERQVPGAEIGLTHNVGGNGQVCCVNVARRAR
                     "
     gene            1859..2746
                     /locus_tag="VHF88_06830"
     CDS             1859..2746
                     /locus_tag="VHF88_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161518.1"
                     /translation="MRTAVLGAGTMGSGIAQAAAMAGHEVTLHDPQPAALARAGEAVR
                     TSLDRFVRSERIAASEADAAERRIAHAATLADAVAEADVVVEAAPESLELKRDVFRAV
                     VAAAPSEALLGTNTSQLSITAIGAAVGDAAERLVGMHFFNPPVMMRLVELVRGLATSD
                     AALERAREFAAGLGKEVVVCRRDSPGFITTRAYAALRQECLRMLEEDVASPEDIDKAL
                     RLAFNFPMGPLELSDFNGIDVYADAVSALAEAHGERFRPPVGVRNMVAAGRLGRKAGH
                     GFYRYDADGKRVEGSGGYG"
     gene            2842..4878
                     /locus_tag="VHF88_06835"
     CDS             2842..4878
                     /locus_tag="VHF88_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX2161519.1"
                     /translation="MIDIGIDVGGTFTDFVFLSDDGGGVRVNKVPTDPADLPGTVLRG
                     LEEIDLAEVSSIVHGTTVAINAVLQHKGAATAIVTTAGFRDHIEIGDTLRYTGGLFDH
                     KWTRTRPYPVPHDRRFEVKERVSPTGAVEIAPDEQDLSAIADELERTGVEAVAICFLN
                     SYQSADNELAAAEYLAKRLPALHITLSSTNPEFREYPRFITAVFNASIAPMLKDYVGS
                     LGSALTSAGYGGEVLYMTSSGGLITEESVVTEPLRLLLGGVAGGVTASAFMAERISAP
                     NVATFDMGGTSSDVGFVKDYRTHVAPNRVLEAFPIALPDLEVNTIGAGGGSIAWLTDD
                     GLIKVGPQSAGAAPGPACYGAGGTDLTVTDANLILGRVGSASLLDGEITLDKDLARAA
                     GERLAQRAGIEDVDELAAGVIEICITNMYGAIREVSVERGENPEEMALIAFGGAGGLH
                     AIPIAERLSMPRVVVPRDAGNFSAFGMLVSDRRYDHVRSYIRTLPRAELAEITQHLGT
                     MGEEGRERLRSDGFGDDDIDIAYKVGMRYQGQIFEEELILRDLDFSVEDLGLWFGDLY
                     EKRYEFRREPEASEIVNLRVVATGRSGHTEVSRASSNGHAGQADALKEMRPVYLRGEF
                     VDTPVYDRSRLVLGATLEGPAVIEEYDSTALLFPDWSAAVDEYENLIFTRSADA"
     gene            4910..7021
                     /locus_tag="VHF88_06840"
     CDS             4910..7021
                     /locus_tag="VHF88_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX2161520.1"
                     /translation="MASVTQDTELNPLTLEVIGADLNYIVREMRSTVIRMAYSPILTE
                     THDFSCAITNRAGEIVAMNVDVPFHMFAVRYAVSTVVDKYGDDIHEGDQFFVNDPWQV
                     GAHLNDTTLLMPYFHDGELRLWLACGVHYGDIGGTVPGSASGECSSIFHEGIRFPAVK
                     ASDRGVLNEDLIEVLLANVRQPFEVKGVYAAQSNCMTLAAKRLNESCERFRPEVVEAA
                     VERLLDNAERQTRRSISELEDGEYHFEEYLENGGVHHPDPVYFACKMTVAGDQLTFDF
                     SGSSDQIDGVANGTLVNTWAAVFDVVDTYLAPGNVSNSGAARALEVTAPEGTVVNSKF
                     PVPINGYATVMFGPIHGCVIGCLAQVMPDEVSGLTTSSANQTNIGGTVGDFRESDVYW
                     LVYEYPSGGWGATKDTDGSLMAYQWHIGDIPTIWPIERLEALNPIRAHFNDSYVDSGG
                     PGYRRGGVGIRRAWEVTSPAEFAVLGTDAVLPRAGICGGYAGALNWISVIRDGKPVKT
                     SELPLKAGGFKLLPGDIVLYLVSGGGGYGDPLGRPEEWVLEDVRDGYVSVDGAREDYG
                     VVIEGDAVDTAATADLRQRMVDERLHVEVTEADEDHYDELMRRRALLSPDFAERLGVG
                     ADDLVEYVNPRGAHVRAWVTIDDSLSGEQSPLGPKGRSICNASAGSRVYVRRPYTHAM
                     RRQPEDLAAKLPLGRLFAQAA"
     gene            complement(7083..7901)
                     /locus_tag="VHF88_06845"
     CDS             complement(7083..7901)
                     /locus_tag="VHF88_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2161521.1"
                     /translation="MTAEAGDDRPADLPGEDPPAVAVEHDGAVALARLNRPRQRNALS
                     NDVLELLAGELRNLDRDPAVRCIVVAGSDKVFASGADVRALAGQDAAGLYFGRRFELW
                     DELRRLRTPTVAAVSGYCLGGGCELALLCDVVVASETARFGLPETGLGLIPGAGGTQR
                     LTRAIGKAKAMDVVLSGRLLSATEAEQAGLVARVAPAGEWLATAHELARAVAARAPVA
                     QLLAREAVGRAFETSLEAGLDVERRAFAQAFATDDAREGIAAFLAKRDPVWQGR"
     gene            complement(7918..>8544)
                     /locus_tag="VHF88_06850"
     CDS             complement(7918..>8544)
                     /locus_tag="VHF88_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2161522.1"
                     /translation="ADGGRAIGLVADVSSAAELDATVAAVLERLGSIDVLYANAGVPG
                     EGAAVEVDPGDWDRTLAVNLTGVFLSARAVLPAMIERGGGCVLAQASAAGLAALPALA
                     PYSAAKAGVIGLTRQLALDYAPHRVRVNAICPGTVVTPLVEAAMERRGGVEQGLERSR
                     ERIPLGRLGKPEEVAALAAFLASDEASWITGAAIPIDGGLTLAARVHH"
BASE COUNT         1219 a         3124 c         3084 g         1117 t
ORIGIN      
        1 gacgccggcg ccgaggccgg cgacaccggg gcggccggca gcctgctggc gctggcgagc
       61 gcgtggcagg gcgccgagcg tggagaccgc tgcgcggcgc tggcctacga gtccggcgcc
      121 ggcgccgacg cgctgctcgt cgaggcgacg gccccgccgc cgccgacggc aacccagccg
      181 gcgcgacggc cggtcggcta tgtcgagctg ctgcggcgcc gcgacctgct cgagccgccg
      241 ccgccgccgg atccgagcgc gccctacgcg gcgacgccgg ccgccctgcg cgacgctccc
      301 tacgtcgacg acctgaccgg actcgagtgc gccgtctgcg gctcgctgaa cttccccgcg
      361 cgaccgacct gcatcgactg cgggtcgagc gagctgtcgc cggtgccgat gccgcgcacg
      421 ggccgcgtcg tgacccacaa cgagcagttc gtcgtcggcg tggcgcccga gccggcaccc
      481 gtgtcggtcg gcgtcgtgcg actcgacggc gcgagcgggg agcgcggcgg gcaggtatcg
      541 gcgatgttcg tcgacggcga cgccgcgatc gagatcggcg cgcccgcgga gctcgtccac
      601 cgccgcctcg gcagcgagcg cggcctggtc aagtacggct ggaagctgcg gatcgtgggg
      661 gagggggcat gagccgcgac ctcggcgtgt cggtcgtcgc tgccgggatg acgcgcttcg
      721 gcgagcgctt cgacgccagc tacgaggagc tcgccgccga cgcctacggc gcctgcctcg
      781 agagctcgga cggcaggatc gacggcgagc ggctcgaggc agcctggctg tcgacagcgc
      841 tcggcgggct gatccgccgc gaggcggtga ccggcgccag cctcgccgat ccgctgcgct
      901 tcttcccgcg gccggtgacg cgcgtcgaga acgcctgcgc cggcggcagc gacgcgatcc
      961 gcaacgcggc gttctcggtc gccgccggcg tctacgacat cgtgctcgtg ctcggcgtcg
     1021 agaagatgcg cgacatcccg acgcgcgcca gcctaatcgc ccagcgcggt cagatgtcgc
     1081 acctctggtg gcacccgcgc ggcgccagct cgccgtacct gttcgcccag cacgcgacag
     1141 tccacatgga gcgcaacggg ctgacgcgcg agcacctcgc ccgcgtcgcc gccaagaacc
     1201 gccgccacgg ggcgctgaac ccgaaggcct tcatgcggcg cgagatcgag gtcgaggatg
     1261 tcctcgacgc gccgatggtc tgctcgccgc tcggcctgct cgactgctgc ccgacgaccg
     1321 acggcgcggc ggcggtcgtg ctggcgcgct ccgacctcgc ggccgagttg accgacgcgc
     1381 cggtcgagct cgccgggctc gggctcgcga ccgacgtgcc gtactcgcac tggaagccgt
     1441 ccttcgagta ccagccggcg ccgacccgcg ccgccgagat cgccttcgag atggccggca
     1501 tcgcgccggg cgacgtcgat gtcgccgagc tgcacgactg cttcagcgtc gcggaggtcc
     1561 tcgcctacga ggacctcggc ttctgcggtc gcggcgaggg cgggcgctgg ctcgactcgg
     1621 gcgcgccgga gctcggcggc gagctgccgt gcaacaccag cggcggcctg ctcgccaagg
     1681 gccatccgat cggcgcgacc gggatcgcgc agatggccga ggtgttctgg cagctgcgtg
     1741 gcgaggccgg cgagcgccag gtccccggcg ccgaaatcgg gctgacgcac aacgtcggcg
     1801 gcaacggaca ggtctgctgc gtgaacgtcg cgcggcgggc gcgctgagag gaggacggat
     1861 gcggacggcg gtgctcgggg cggggacgat gggcagcggc atcgcgcagg cggcggcgat
     1921 ggcgggccat gaggtgacgc tgcacgatcc ccagcccgcg gcgctcgcac gcgcgggcga
     1981 ggcggtccgc acgagcctcg accgcttcgt gcgctcggag cggatcgcgg cctccgaggc
     2041 cgacgccgcc gagcgacgga tcgcccatgc cgcgacgctg gccgacgcgg tcgccgaggc
     2101 cgacgtcgtc gtcgaggcgg cgccggagtc gctcgagctc aagcgcgacg tcttccgcgc
     2161 ggtcgtcgcg gcggccccgt ctgaggcgct gctcggcacg aacacctcgc agctcagcat
     2221 cacggcgatc ggcgcggcgg tcggcgacgc cgccgagcgc ctggtcggca tgcacttctt
     2281 caacccgccg gtgatgatgc ggctggtcga gctcgtgcgc gggctggcga cgagcgacgc
     2341 cgcgctcgag cgggcgcgcg agttcgccgc cggactcggc aaggaggtcg tcgtctgccg
     2401 gcgcgacagc ccgggcttca tcaccacgcg cgcctacgcg gcgctgcgcc aggagtgcct
     2461 gcggatgctc gaggaggacg tcgcgagccc cgaggacatc gacaaggcgc tgcggctggc
     2521 gttcaacttc ccgatgggcc cgctcgagct gtcggacttc aacggcatcg acgtctacgc
     2581 cgacgcggtg agcgcgctcg ccgaggccca cggcgagcgc ttccggccgc ccgtcggggt
     2641 gcgcaacatg gtcgcggcag gacgcctcgg gcgcaaggcc gggcacggct tctaccgcta
     2701 cgacgcggac gggaagcggg tcgagggatc gggcggctat ggctgagtgc gctcttgcct
     2761 tgttagtaca atcatgataa gagtatgccg gcaagccggc ggcaacgccg acacaccctc
     2821 cggggagagg agcttcgaga gatgatcgac atcggcatcg acgtcggcgg gacgttcaca
     2881 gacttcgtct tcctgtccga cgacggcggc ggagtgcgcg tcaacaaggt tccgaccgac
     2941 ccggccgacc tgcccggcac cgtcctgcgc gggctggagg agatcgacct cgccgaggtg
     3001 tcctcgatcg tccacggcac gaccgtcgcg atcaacgccg tcctgcagca caagggcgcc
     3061 gcgacggcga tcgtcacgac cgcgggcttc cgcgaccaca tcgagatcgg cgacacgctc
     3121 cgctacaccg gcgggctctt cgaccacaag tggacccgca cgcgccccta cccggtgccg
     3181 cacgaccgcc gcttcgaggt caaggagcgc gtctcgccga ccggcgcggt cgagatcgcg
     3241 cccgacgagc aggacctctc ggcgatcgcc gacgagctcg agcggaccgg ggtcgaggcc
     3301 gtcgcgatct gcttcctcaa ctcctaccag agcgccgaca acgagctggc cgccgccgag
     3361 tacctcgcca agcggctgcc ggcgctgcac atcacgctct cctcgaccaa ccccgagttc
     3421 cgcgagtacc cgcgcttcat caccgccgtc ttcaacgcct cgatcgcgcc gatgctcaag
     3481 gactacgtcg gcagcctcgg cagcgccctg accagcgccg gctacggcgg cgaggtgctc
     3541 tacatgacct cgtcgggcgg actgatcacc gaggagtcgg tagtcaccga gccgctgcgg
     3601 ctgctgctcg gcggcgtcgc cggcggggtc accgcgtcgg cgttcatggc cgagcggatc
     3661 tctgctccga acgtcgcgac ctttgacatg ggtgggacca gctccgacgt cggcttcgtc
     3721 aaggactacc gcacccacgt cgcgccaaac cgcgtgctcg aggcgttccc gatcgcgctg
     3781 cccgacctcg aggtcaacac gatcggcgcc ggcggcggca gcatcgcctg gctgaccgac
     3841 gacgggctga tcaaggtcgg cccgcagagc gcgggcgccg cgccgggacc ggcctgctac
     3901 ggcgccggcg gcaccgacct gaccgtcacc gacgcgaacc tgatcctcgg ccgcgtcggg
     3961 tcggcgtcgc tgctcgacgg cgagatcacg ctcgacaagg acctcgcgcg cgcggccggc
     4021 gagcggctcg cgcagcgcgc cgggatcgag gacgtcgacg agctcgccgc cggggtgatc
     4081 gagatctgca tcacgaacat gtacggcgcg atccgcgagg tctcggtcga gcgcggcgag
     4141 aacccggagg agatggcgct gatcgccttc ggcggggccg gcggcctgca cgcgatcccg
     4201 atcgccgagc ggctttcgat gccgcgcgtc gtcgtcccgc gcgacgccgg caacttctcg
     4261 gccttcggga tgctcgtctc cgaccgtcgc tacgaccacg tgcgcagcta catccgcacg
     4321 ctgccgcgcg ccgagctggc cgagatcacc cagcacctcg ggacgatggg cgaggagggg
     4381 cgcgagcggc tgcgctcgga cggcttcggc gacgacgaca tcgacatcgc ctacaaggtc
     4441 gggatgcgct accagggcca gatcttcgag gaggagctga tcctcaggga cctcgacttc
     4501 agcgtcgagg acctcggcct ctggttcggc gacctctacg agaagcgcta cgagttccgc
     4561 cgcgagcccg aggcgtcgga gatcgtcaac ctgcgcgtcg tcgcaaccgg gcgcagcggc
     4621 cacaccgagg tcagccgcgc gagctcgaac ggtcacgccg gccaggccga cgcgctcaag
     4681 gagatgcgcc ccgtctacct gcgcggcgag ttcgtcgaca cgccggtcta cgaccgctcg
     4741 cggctcgtac tgggggcgac gctcgaggga ccggcggtga tcgaggagta cgactcgacg
     4801 gcgctgctct tccctgactg gtcggcggcc gtcgacgagt acgagaacct gattttcacc
     4861 cgctcggcgg acgcgtgagc gcgctcgccc ggacgatcgg agactgccaa tggccagcgt
     4921 gacccaggac accgagctca acccgctcac cctcgaggtg atcggcgccg acctcaacta
     4981 catcgtgcgc gagatgcgca gcacggtgat ccggatggcg tactcgccga tcctcaccga
     5041 gacgcacgac ttctcgtgcg cgatcaccaa ccgcgccggc gagatcgtgg cgatgaacgt
     5101 cgacgtgccg ttccacatgt tcgccgtccg ctacgcggtc tcgacggtgg tcgacaagta
     5161 cggcgacgac atccacgagg gcgaccagtt cttcgtcaac gatccctggc aggtcggcgc
     5221 ccacctcaac gacacgacgc tgctgatgcc gtacttccac gacggcgagc tgcggctctg
     5281 gctcgcctgc ggcgtccact acggcgacat cggcggcacc gtcccgggca gcgccagcgg
     5341 cgagtgctcc tcgatcttcc acgaggggat ccgctttccg gcggtcaagg cgtccgaccg
     5401 cggcgtgctc aatgaggacc tgatcgaggt gctgctggcc aatgtgcgcc agccgttcga
     5461 ggtcaaaggc gtctacgcgg cgcagtcgaa ctgcatgacg ctggccgcca agcggctcaa
     5521 cgagtcatgc gagcgcttcc gcccggaggt cgtcgaggcg gccgtcgagc ggctgctcga
     5581 taacgccgag cgccagacgc gccgcagcat ctccgagctc gaggacggcg agtaccactt
     5641 cgaggagtac ctcgagaacg gcggcgtcca ccaccccgac ccggtctatt tcgcctgcaa
     5701 gatgacggtc gcgggcgacc aactgacgtt cgacttcagc ggctcctccg accagatcga
     5761 cggcgtcgcc aacggcaccc tggtcaacac ctgggcggca gtcttcgatg tcgtcgacac
     5821 ctacctcgcg cccggcaacg tgtcgaactc gggcgccgcg cgtgcgctcg aggtcaccgc
     5881 gccggagggg accgtcgtca actcgaagtt ccccgtgccg atcaacggct acgcgaccgt
     5941 catgttcggg ccgatccacg gctgcgtgat cggctgcctg gcgcaggtca tgcccgacga
     6001 ggtctcgggc ctgacgacct cctcggccaa ccagaccaac atcggcggga cggtcggcga
     6061 cttccgcgag agcgacgtct actggctcgt ctacgagtac ccgtcaggcg gctggggggc
     6121 gaccaaggac accgatggca gcctgatggc ctaccagtgg cacatcggcg acatcccgac
     6181 gatctggccg atcgagcggc tcgaagcgct gaacccgatc cgcgcccact tcaacgactc
     6241 ctatgtcgac agcggcggcc ccggctaccg ccgcggcggc gtgggcatcc ggcgcgcttg
     6301 ggaggtcacc tcgccggccg agttcgccgt gctcggcacc gacgccgtcc tgccgcgcgc
     6361 cggcatctgc ggcggctacg cgggcgcgct gaactggatc tcggtgatcc gcgacggcaa
     6421 gccggtcaag acgtcggagc tgccgctcaa ggccggcggc ttcaagctgc tgccgggcga
     6481 catcgtcctc tacctggtca gcggcggcgg aggctacggc gacccgctcg ggcggcccga
     6541 ggagtgggtg ctcgaggacg tgcgcgacgg ctacgtgagc gtcgacggag cgcgcgagga
     6601 ctacggcgtg gtgatcgagg gcgacgccgt cgacaccgcc gcgaccgccg acctgcgcca
     6661 gcggatggtc gacgagcggc tgcacgtcga ggtcaccgag gcggacgagg accactacga
     6721 cgagctgatg cggcgccggg cgctgctcag ccccgacttc gccgagcggc tcggcgtcgg
     6781 cgcggacgac ctcgtcgagt acgtcaaccc gcgcggcgcg cacgtgcgcg cctgggtgac
     6841 tatcgacgac tcgctctccg gcgagcagag cccgctcggg ccgaagggac gcagcatctg
     6901 caacgcctct gcgggctcgc gggtctacgt ccgccgtccg tacacccacg cgatgcgccg
     6961 ccagccggag gacctggccg cgaagctgcc gctcgggcgc ctgttcgcgc aggctgcctg
     7021 agacggctac cgacccgccc cgtcccgctc cctccctcct cgcgggcggg gcgggtcgcc
     7081 cctcagcggc cctgccagac cgggtcgcgc ttcgcgagga aggcggcgat gccctcgcgt
     7141 gcgtcgtcgg tcgcgaacgc ctgggcgaac gcgcgccgct cgacgtcgag accggcctcg
     7201 agcgatgtct cgaacgctcg cccgaccgcc tcgcgcgcga gcagctgcgc gaccggcgcc
     7261 cgcgccgcga cggcgcgggc cagctcatgt gcggtcgcca gccactcccc ggccggcgcg
     7321 acgcgggcga ccagcccggc ctgctcggcc tcggtcgccg acagcagccg gccgctgagc
     7381 accacgtcca tcgccttcgc cttgccgatc gcgcgcgtca gccgctgcgt cccgccggcg
     7441 cccgggatca gcccgagacc ggtctcgggc aggccgaagc gggccgtctc ggaggcgacg
     7501 acgacgtcac agagcagcgc gagctcgcag ccgccgccga ggcagtagcc ggagaccgcc
     7561 gcgaccgtcg gcgtgcgcag gcggcgcagc tcgtcccaga gctcgaagcg gcggccgaag
     7621 tagaggccgg ccgcgtcctg gccggccagc gcacggacat cggcgccgga ggcgaagacc
     7681 ttgtcggagc cggcgacgac gatgcagcgg accgcggggt cgcggtcgag gttgcgcagc
     7741 tcgccggcga gcagctcgag cacgtcgttg gagagcgcgt tgcgctggcg cggccggttc
     7801 aggcgcgcga gcgctacggc cccgtcgtgc tcgaccgcca cggcgggcgg atcctcgccg
     7861 ggcaggtcgg cgggtcggtc gtcgccggct tcggcggtca tcggcagctc ctcggggtca
     7921 atggtggacg cgggcggcga gcgtcagccc accgtcgatc gggatcgcgg cgccggtgat
     7981 ccagctggcc tcgtcgctcg ccaggaaagc cgcgagcgcg gcgacctcct cgggcttgcc
     8041 gagccggccg agcgggatcc gctcgcgcga ccgctcgagc ccctgctcga cgccgccgcg
     8101 gcgctccatc gcggcctcga cgagcggggt cacgacggtg ccggggcaga tcgcgttgac
     8161 gcgcacgcgg tgcggcgcgt agtcgagcgc gagctggcgc gtcagcccga tcacgccggc
     8221 cttggcggcg ctgtaggggg cgagcgccgg cagggcggcg agccctgccg cgctcgcctg
     8281 ggcgagcacg cagccgccgc cgcgctcgat catcgccggc agcacggcgc gggcggagag
     8341 gaagacgccg gtcaggttga cggcgagcgt gcggtcccag tcgccggggt cgacctcgac
     8401 cgccgctccc tcgcccggca cgcccgcgtt ggcgtagagg acgtcgatcg acccgagccg
     8461 ctcgagcacc gcggccaccg tcgcgtcgag ctccgccgcg gaggagacgt cggcgacgag
     8521 gccgatcgcc cggccgccgt ccgc
//
LOCUS       DATABV010000156        21573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_530812, whole genome shotgun sequence.
ACCESSION   DATABV010000156 DATABV010000000
VERSION     DATABV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21573
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530812"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VHF88_06855"
     CDS             <1..207
                     /locus_tag="VHF88_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161523.1"
                     /translation="GGADPRLTLVARLVSILRHAWTPAGVVAWFDRPRRELDGRAPRE
                     LLDDPANERILIATARSSRNQYGS"
     gene            211..819
                     /locus_tag="VHF88_06860"
     CDS             211..819
                     /locus_tag="VHF88_06860"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX2161524.1"
                     /translation="MGRFSFLQFQGTVYRATSYDTPLWVLPNRRSGRWNDPVDETIVQ
                     YCTLDAAAPLAEFVRHEDLREAADAAEARLSVWQLRLAEGAIVDLSSPERATAQEVDW
                     DALVADDWKACQELGREIAAAGGRGILGPCAALPGSLSLTIFGPRSEIRWTAEPRLAI
                     QIPARKIMHGAPGAGIVAGTRFFGMPYPDGVATPAVGHLLDR"
     gene            complement(840..1664)
                     /locus_tag="VHF88_06865"
     CDS             complement(840..1664)
                     /locus_tag="VHF88_06865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX2161525.1"
                     /translation="MTDGYFTDSSMLRIVHRERALALSGPRALLMQAAHPLAVKGLLA
                     HSTAVDEPYDRLARTAETMSTIGFGSREAADAVTARVRAMHARVRGRTTEPVGRHPAG
                     TPYRADQPDLLLWVLFTLVDSGAVVYRKYVRSMSRAQEAAYWEDYKVVGELFGLERAD
                     MPDTLDDLHAYRHDMLTGDDLHVTDWAREKARKIVLEPPVPWVARPLLETANFITIAL
                     LPDRIRREYGFSPLPPVAVRRLLVAGGAEYVKRAVVPFLPDRLRLVPAARGPSAAA"
     gene            1738..2478
                     /locus_tag="VHF88_06870"
     CDS             1738..2478
                     /locus_tag="VHF88_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX2161526.1"
                     /translation="MPAAAYAGKECVCQFKRGICDQTCVRDMLDTPFYIACLKLSGRR
                     CVVIGGGEIGLEKVEGLLACDGDVTLIAPEAGGELEALAAEGSIRWERREYAGPDDLE
                     GVFMVIACTDSTETNIRIYEDAERRAMLVNIVDVPPLCNFILPAIVRTGPLAIAISTA
                     GASPALAKRMKREVSELFGEEYARLAIMLNDVRGWAKGTLPTYQDRKAFFESIVNGTP
                     DPIELLRAGDEAAVRDLIAAAQRTAVPA"
     gene            2519..3100
                     /locus_tag="VHF88_06875"
     CDS             2519..3100
                     /locus_tag="VHF88_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX2161527.1"
                     /translation="MRTSPPDALPIFRSDLQARLLAALFLNDGAPLTTRELAERTAAK
                     PATLHRELGRVEDAGLITHESVGRTKRYRAATESPLHRPLRELLERTLGVEPLLRRGL
                     AEVEGVERAAIFGSWAEGRSDAGSDIDLLVIGDIDRDALLAAVREVERQVHREIDVTA
                     YRPDEFERRRGDGSGFLRTVLRGPLIQLVGEVL"
     gene            3097..3498
                     /locus_tag="VHF88_06880"
     CDS             3097..3498
                     /locus_tag="VHF88_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX2161528.1"
                     /translation="MTRAKVREVEPDSVAAAELIAQARAHLDSARLAGIDAQSAYGIC
                     YQAALKAMVAALLADGRRVTSGAGGHIVTIQEAEARLALDPTVSVRIDHMRRTRHRVF
                     YDGHEVSELELEGALADAEAVVAAAAEAVDA"
     gene            3594..4262
                     /gene="ccmA"
                     /locus_tag="VHF88_06885"
     CDS             3594..4262
                     /gene="ccmA"
                     /locus_tag="VHF88_06885"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX2161529.1"
                     /translation="MELDRLERRYGDRVALSGVTVRLAVGETLTVLGSNGAGKTTLLR
                     VLATLLRPHDGTAQVLGYELPGEAWRVRGAIGYLGHDPLLYRELTGRENLRYHARLHG
                     TEGARVEERLEAVGMARRADEPVRELSKGMVQRLAVARATLHDPQLLLLDEPRANLDP
                     AAAEALEPLIGRASGKARVLVTHDVEGGLAEADVALGLRGGKQVFCSPAAEVDTATAR
                     GLYS"
     gene            4259..4933
                     /locus_tag="VHF88_06890"
     CDS             4259..4933
                     /locus_tag="VHF88_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX2161530.1"
                     /translation="MIATARAILRKDLAIELRTKESVPAMTLFTVTVFVLFHFGLDRD
                     SLDGGLASGVLWVTLLLAAVIGVSRLFAVEREQGGIDGLLLAPVDRTALWLAKTAALF
                     LYLCLLELVAVPAFALFLLGPDLLPALPVLIPLLLAANVGLAAIGALVSALAAETRAH
                     ELIVPLLLLPLVVPLLIAAASATEPLLRGDQAPEDLGRWLGLLSLYDVVFVLIAVAVF
                     DFLLDD"
     gene            4934..5626
                     /gene="ccsA"
                     /locus_tag="VHF88_06895"
     CDS             4934..5626
                     /gene="ccsA"
                     /locus_tag="VHF88_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX2161531.1"
                     /translation="MYSKGLKPLSIATVVLMAASLALVFFFAPNDADQGFVQKIFYVH
                     VPLAIVALCGFVAGGIMAIKFLRSGDRSWDARSYVAIHMSIIFGTAVLITGAIWAKAA
                     WGHWWVWDEPVLVSFLVVFLLYCVYYPLRFSIEDPDRQARYSSVFAIAAGAFVPINFA
                     AVRMAEAFTHPRVLSQTGGSMPGDMRLTFLVSIAAVTLLFVTLWKLELTGKHASMQLK
                     RLRRKLEAEATG"
     gene            5626..5799
                     /locus_tag="VHF88_06900"
     CDS             5626..5799
                     /locus_tag="VHF88_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161532.1"
                     /translation="MPALPLDEAGKYVAGAYVVFFTLIVVYVAIISAKIARIDRELTE
                     LVEIAERDEEPVA"
     gene            5796..7232
                     /gene="hemA"
                     /locus_tag="VHF88_06905"
     CDS             5796..7232
                     /gene="hemA"
                     /locus_tag="VHF88_06905"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX2161533.1"
                     /translation="MSGELLALGVSHKTAPLELRERLALTEGRATAVLAELTAHEEIH
                     EAVAISTCNRTELYLVATDAVAAESAALAVLARQAETRPTELADSLYALRDLDVARHL
                     FQVAAGLDSMIVGESEIQGQVKRAYELALVEGMTGPIANRLFRDALAAGKRVRTDTGV
                     GRSRVSVATVAAELACRSLGIVGGATPTVTDARCPASGGDLPAHHPSTPDLSDRRVLV
                     IGAGEQGELTAQALKDRGAHAVFVANRHYDRALGLAERYSGSAVPLDELPNELAQADI
                     VVSCTSSPHQLVGREELALVLEGRDDRPLVIIDIAVPRDIDSGVRELSGVTLYDMDDL
                     EHEVERNISVRRAEAERALALVERDVERFEGWLASLEVLPTIRGLRERADAIVAQVLA
                     ENAPRWESLSDADRERVAAMSRALVARLFHEPTMRLRDGAEDESSYIHVAVLRELFGL
                     DPGPAPLDEDEQPSAEVTQLDSRRRRRG"
     gene            7242..10079
                     /gene="hemC"
                     /locus_tag="VHF88_06910"
     CDS             7242..10079
                     /gene="hemC"
                     /locus_tag="VHF88_06910"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX2161534.1"
                     /translation="MATPLRVGTRGSALAVAQSTAVARDLEALGAEVELVTIRTSGDE
                     TAPKPDTEDKARFVKELEQALLDGEIDLAVHSAKDVPGELPDGLEIVAVPERADARDA
                     LCLSPGLLDRRGPAETTGAEPTTATDRPADRSPSTSPDASVNHSPAAQPLGALPPGAT
                     VGTASLRRRAALLALRPDLDVRELRGNVDTRLRRLADGELDAIVLATAGLIRLGRSDS
                     GTPLPELTLVPAAGQGCLALEARVGDDSTRRLACRLTDPRAFACLTAERTLVTAIDAT
                     CHTPVGAHATIENHTILRLTAFVGTPDGATWIRDEIRGLAEGPQAVGADAADRLLAAG
                     AADILSSAEETPLRELARPIAPRRTPRCSVRQMAAGIVYLVGAGPGDPGLMTRRSLEL
                     IAAADAILYDRLIPADALDGARADCELRYVGKAPGDVAMSQEDICALLVELGLAGRRV
                     VRLKGGDPFVFGRGGEEAEALRAAGVPFEVVPGITAGVAAPAYAGIPVTHRDAATAVA
                     FVTGHEAPEKTRARGDGDGPERPHSEVDWQGLAAFPGTLVFYMGVKNLPLIAEALIAA
                     GREASEPAAVVRRGTLPDQRTVTGTLADIAERVAQAGLKAPAITVVGPAVGLRDTLAW
                     IEHRPLHGRRVAVTRARAQASGLAARLRALGAEVVETPAIRIQPRPVEGEIVEAIEAL
                     RGASISPEIPASAAPGDDAAYSLVCFTSTNGVGLFFEALAARGLDARALAGSIVAAIG
                     PGTARELEQHGVRADVVPPRSVAEALVEALADAPVEGRRVLVARAGQGRDVLPDALRE
                     RGADVDVVGLYDTVVEPLSDEQRAALADVDYVTFTSASSVRNLVESLTAGRRPPDEGF
                     ASPGRPKPTSDEAGGGTVRADEGSASPRLHKPASGDGPLGRARIVSIGPVTSEQAREL
                     GLSVDVEAERHDVDGLIDALVVDAAATPVTP"
     gene            10076..10924
                     /locus_tag="VHF88_06915"
     CDS             10076..10924
                     /locus_tag="VHF88_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX2161535.1"
                     /translation="MTNAPIVSLLTDYGRDDEFVGVCHGVILKIARDARIVDVSHEIP
                     RHDVRRGAIVLRNALPYVPADVHVAVVDPQVGSERRALAVRCEDGTQLVGPDNGVLSL
                     AWDRCGGVDIAVDVTRSPHRLEPVSATFHGRDLFVPVGAHLAAGAEIADAGEPVDPAT
                     LDRIALPHPLFEDDGTIVAHVVTIDAYGNVALDLTHDQLAGTGLMLGVTAEIEVDSGD
                     GGAAVKLGGGRIAAPFVHTFADVGPHEAILYEDASRAVAIAINRGDAAAELGLRPDDE
                     VRLRPR"
     gene            10935..11627
                     /locus_tag="VHF88_06920"
     CDS             10935..11627
                     /locus_tag="VHF88_06920"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX2161536.1"
                     /translation="MIGEPRIHHPVTDSTNERARELASEGAPHGTLVTADEQTAGRGR
                     QGRTWTAAPGEALLMSVIVRDLEPRHALLPLMAALAVCDAAEAVSDVRCEIKWPNDVW
                     VDRRKLSGILVEARPAAGWAVVGIGLNTGVREFPEELRETATSLHLPEPDVALPPLLA
                     ALDRWLAADQADILAVWRRRDALAGSPVRWQDGHGTARGVDDSGSLLVETPGGELLAL
                     GAGEVHLRPPPG"
     gene            11653..12456
                     /locus_tag="VHF88_06925"
     CDS             11653..12456
                     /locus_tag="VHF88_06925"
                     /inference="COORDINATES: protein motif:HMM:NF025864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HEX2161537.1"
                     /translation="MSKLRRARLKQRLDPVAFDSIATETDELLERRLRKAKRLTDVAS
                     PDVNLNPFLMLALAPAYNIASPFEAAEYVQLSKMLHGDSTAFGRYVEDKILPVFGVRP
                     VAEKKRSPKLYSPIDAEVSVEGKRYLMTLKAGPWTMNQSHANEMVNRFPEIHDKTGDD
                     IVIGIFYGSRSRLNNKPRLVEVNTGDYVTTLVGKDFWEFVTGVKDAHQEIFRAIREAQ
                     SRFAARHGGETFYEQMIEARLALSRSIREAFGLGAEDIDMWEQLFEGAF"
     gene            complement(12453..13694)
                     /locus_tag="VHF88_06930"
     CDS             complement(12453..13694)
                     /locus_tag="VHF88_06930"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX2161538.1"
                     /translation="MRAISLFTGAGGLDIGVERAADCELLLAVEFDPMAVEALRDNVE
                     RGLLGSSRTVVRRADLATLDPRTARRELGLKRGELDLLVGGPPCQSFSTAGRRRTVQD
                     PRGTMIWSYLRWVEEFQPRFFLLENVRGLLSAALRHRPIAERPVHGGPPLALDEEPGS
                     VVAAWIDDLRAADPAYRVDCFEVNAVNYGAPQLRERVLLVGNREGKVVRFPEPTHGVA
                     PGLQPYETLADALRGLEEDAPVIMDFSARKKAYLGKVPPGGNWRALPERLQRESMGRA
                     FHAKGGRSGWWRRLSWDLPCPTIVTMPNHASSSLCHPDEVRTLTVRECARVQGFPDEW
                     GFSGTPQQQYAQVGNAVPIRLAEVAGEAIRDARAASPEPVAALEPFRRVYLKSHVRTR
                     RWYKDGDAYLRADGADVAVPA"
     gene            complement(13735..14130)
                     /locus_tag="VHF88_06935"
     CDS             complement(13735..14130)
                     /locus_tag="VHF88_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEX2161539.1"
                     /translation="MQGNRGADTSPEVALRRRLHQMGLRYRVGLAPIAGFRCRPDIVF
                     TLARVAVFVDGCFWHGCPEHGRVPKSNSGYWAAKLRRNVERDRRQDAVLSAEGWAVVR
                     VWEHDDVHAAAARIGRLVRANRLTVRPGI"
     gene            complement(14203..16728)
                     /locus_tag="VHF88_06940"
     CDS             complement(14203..16728)
                     /locus_tag="VHF88_06940"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX2161540.1"
                     /translation="MAESTSVAATAPVSGDDLERAEAFAREPFVLEGEDRDAALAPGT
                     VRRAALDDALAEIADGAERPSAEWKRRWSLVLGLERVLADDEPKLVDGTVLSAHQVDA
                     LSGTLIALTAEVQSKSNGKVAPAALEELPSGEIAIDGDDDEDDSDDDEPLDWDANEEA
                     EEEAAADSVEDDPGAARRFWFEHATGAGKTVAALGFVEASRTGGVLILTHRRNLVDQF
                     NGELTDRGYKKRISPPLLGRDKARVEGPVTVETYQWYVRNAGKISDDYTVVICDEAHT
                     ALGEKTSASIRQWDGPVFVGMTATGALIARHVTDLFPTQTSRFDLAQAARRGVIAPLR
                     CVRIPPGAGVRSISKVPLRKGEVDQDFDQEELAALLDQTPFNLAIADLYKTRFKNLPG
                     VVYSAGVAHAHNVADAFVAAGIKAKAVSGETPKRELTRILSDYELGKVDVLVNAQLLA
                     EGWNSPRATVCLHLAPTASKRIYQQRVGRVTRRHQGKEAGIVVDFVHPATTNDDPVVT
                     LHSLLDRDVYRGGAIVVGPVRRGRGRRLRVERRVVPVTADDERRAQVFERELWRIAVE
                     HLSWGEQHAWAALAGARVASNNWRRAKAMLHFDSTGELRRRFLIECLQRNRNSQLRVR
                     ALQEIGQIKDPEAFDVAVEIVGTWQRDERREGVKTLLQAMAERPMGRRDQAARWIWHL
                     AEYSREVHEEYAVSRWPETKRLLGLLVNSSGGAHARNARRLVHATKKQDRRLSAALLA
                     AALAHTPEAQQVLDGARMRLGRKPSALARELLRNFPKGRRKRSGRRRKKGGGDGSADG
                     DKAAEAQEQQHDASANGSADTEPVPSKAAEPGPEAEDEVAERL"
     gene            16927..17466
                     /locus_tag="VHF88_06945"
     CDS             16927..17466
                     /locus_tag="VHF88_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2161541.1"
                     /translation="MSFVTVVGVATGCGGDDPQSAAPSQEATRDALKGAPAPLAAVHA
                     QANELLDGGPKAFKQRLAELRGHPVVVNKWGSWCGPCREEFPYFQRQAVEHGKRVAFL
                     GVDVQESKAEGRELLEEFPVSYPSYWDPRLTISAVFNGVAATPATAFYDSKGKLAYLK
                     QGPYLSDRDLAEDIQRYAR"
     gene            17471..18313
                     /locus_tag="VHF88_06950"
     CDS             17471..18313
                     /locus_tag="VHF88_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161542.1"
                     /translation="MQELRIDPLSGLRVAIGELDASAPQAPGETAAETDDPLAGGRGQ
                     PEMFAVGRAGAGTVETIDSVPGPVGSLLELGADGLEETMGLWRERMRAHDGVACRHLI
                     VSEGEAAGASLPHSHAQLFALPFVPAALARERERFTAYHQRTQGRNLLEDLLTEEVRR
                     GDRLVVVDREAIAICPFASAAPFQLQLIPRRPAARFEDDGPLGAAALHGALERLSAVL
                     GELPPFNLWVRTAPSGAESFCWRIDLLPRVAPPGGLELGTGVRLNAVSPEDAAERLRA
                     AAAA"
     gene            18310..19227
                     /locus_tag="VHF88_06955"
     CDS             18310..19227
                     /locus_tag="VHF88_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX2161543.1"
                     /translation="MIPIALTIAAATAAGFGVEHRLGELRSQHVAELVTRLMLWILLP
                     PIVFFNIADLHLTAGVGLGVIFGWVGLGVAMLAAYAIGKWVLHLSRASTGTLMLAAGL
                     SNTGYLGLPFTAALFGFDDVGDAIVFDILVSGIALLTVGFGIGAAFGTVAEARRDRIV
                     SFFTRNPGIWAGAAAVFAPAALAPEWAVDASRIAVFAMLPLGFFVVGIALASEAEEGA
                     LRFPPPLTAPVGVALGLKLLLFPAIVFGLSRLIIDVPDSYYSQAAMASGINTVLISHT
                     YGMDRSIAGSAIAWSTAIVVATGLVVALL"
     gene            19286..20359
                     /locus_tag="VHF88_06960"
     CDS             19286..20359
                     /locus_tag="VHF88_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX2161544.1"
                     /translation="MIYRTPKIDGGLERRLLELDDLRTRLGQETERPLRWIGTLRRLV
                     KATTVESSTSIEGFSVSPEKAVSLVSGDDRPRAGDEDQMAVASYARAMRHVAAMAADP
                     RFQWSERVLLDLHFDACEFQGDKDPGQWRRGPIQVTGAGGRIAYRGPDGAEVPDLMAE
                     AVAWLQRGDLEAPAIVRAAMAHLHVAAIHPFRDGNGRVARIAQSLVLAREGLVSYEFA
                     SIEEYLGERTQDYYDVLERTQGGSYQPERSAREWVEFCVEAHIDQAQRRLGQIAAAGE
                     RWSRLEAIAESRDWPERLIVALEQGLVGGADRATYAREADVSPATASADLRRLVDAGL
                     LDQRGRGPSSRYLASDALRREIG"
     gene            20391..21002
                     /locus_tag="VHF88_06965"
     CDS             20391..21002
                     /locus_tag="VHF88_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161545.1"
                     /translation="MPPSERPIPMYIAEPPQESAPYGRWEETLRSHFMVAAGAIDTEE
                     EIGEPGALAWFPERTWGGRTFVPCTAPTSEGFELFGYVSYTREHEGAEATDFAAVADY
                     TDETAEANPDWTLDLSDEEIGHWRGPEGRRGVVTLVWGVALVPEGAVATTELGPTTTD
                     QCILADNRFTLISLDAYTGDYVEVRLYAKRGAELARESLYEDE"
     gene            complement(20948..>21573)
                     /locus_tag="VHF88_06970"
     CDS             complement(20948..>21573)
                     /locus_tag="VHF88_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161546.1"
                     /translation="AAVMPSAARSRESTTRRSAGRRITSTPTLRPAGVLWASLAIWSL
                     LACALILANLRALPALLSLSLFRPYVDADEVARWVAKRLGISQPVRATPGDPERRSRT
                     ELLAKVTYFAQPFKRFMSELAEATAPPFFKSFLRVDVDGDRKLTITCFGVTGLPEHED
                     DPSIEHVVGPIALREAQPPPGRAQPAGQPRYSSSYSDSRASSAPRLA"
BASE COUNT         2972 a         7865 c         7648 g         3088 t
ORIGIN      
        1 ggcggggcgg atccacggct cacgctcgtg gcgcggctcg tgtccatcct ccgtcacgcc
       61 tggacgccgg cgggcgtcgt cgcctggttc gaccggccgc gccgcgagct tgacggtcgc
      121 gccccccgcg agctgctcga cgatccggca aacgagcgca tcttgattgc aaccgcgcgc
      181 tccagccgca atcagtacgg gagctgaccg atgggccggt tctccttcct acagttccag
      241 ggaaccgtct accgcgcgac cagctacgac acgccgctct gggttctgcc caaccggcgg
      301 tctggccgct ggaacgatcc cgtagatgag acgatcgtcc agtactgcac gctggacgcc
      361 gcggctcccc tcgcggagtt cgtccgtcac gaggatctgc gcgaggccgc cgacgcagcc
      421 gaggcgcgat tgtccgtctg gcagctgcgc ctggccgaag gggcgatcgt cgacctgtcg
      481 tcacccgagc gggccaccgc ccaagaggtc gactgggatg cgctggtggc cgacgactgg
      541 aaggcgtgcc aggaactggg gcgggagatc gcagcagccg ggggacgcgg catccttgga
      601 ccctgcgcgg cgctgcctgg cagtctctcg ctgacgatct tcggtccgcg ttcggagatc
      661 cgatggacag ccgagccgcg cttggcgatc cagatacccg cgcgcaagat catgcacggt
      721 gcgccgggag ccggcatcgt tgccggtacg cgcttcttcg gcatgcccta tccggacggc
      781 gttgccactc cggccgttgg ccatctgctc gaccgttgac ccggggggcg gtcgcctcac
      841 taagcagcgg cgctcggacc tcgcgcagcg ggaacgagcc gcagccggtc cggcaggaac
      901 gggactacag cgcgcttgac gtactcggcg ccacccgcga ccagcagcct gcgcaccgcg
      961 accggcggca gcggcgagaa accgtactcg cggcggatgc ggtcgggcag cagggcgatc
     1021 gttatgaagt tcgccgtctc gagcagcggc cgggcgaccc acggcaccgg cggctcgagg
     1081 acgatcttgc gcgccttctc acgcgcccag tcggtgacgt gcaggtcgtc gccggtcagc
     1141 atgtcgtggc ggtaggcgtg caggtcgtcg agcgtgtccg gcatgtcggc gcgctccagc
     1201 ccgaacagct cgccgacgac cttgtagtcc tcccagtagg ccgcctcctg cgccctcgac
     1261 atcgagcgga cgtacttgcg gtagacgacc gcgccgctgt cgaccagcgt gaacagcacc
     1321 cacagcagca ggtcgggctg gtcggcgcgg tagggcgtgc cggcggggtg gcggccgacc
     1381 ggctcggtcg tccgccccct cacgcgcgcg tgcatggcgc gcacccgggc ggtcaccgcg
     1441 tcggcggcct cgcgcgagcc gaagccgatc gtcgacatcg tctcggcggt gcgggcgagc
     1501 cggtcgtagg gctcgtcgac cgcggtcgag tgggcgagca gccccttgac ggcgagcggg
     1561 tgggcagcct gcatcagcag cgcgcgcggc ccggagagcg ccagcgcccg ctcgcggtgg
     1621 acgatccgca gcatcgagga gtcggtgaag tagccgtcgg tcatccgcct cccagggtag
     1681 gcgccggcga tccgagcgcc ggccgccggc cgagacggcc cgcgtagact ggaccgcatg
     1741 ccagccgccg catacgcggg caaggagtgc gtctgccagt tcaagcgcgg gatctgcgac
     1801 cagacctgcg tgcgcgacat gctcgacacg ccgttctaca tcgcctgcct gaagctgagc
     1861 ggccggcgct gcgtggtgat cggcggcggc gagatcgggc tcgagaaggt cgaggggctg
     1921 ctcgcctgcg acggcgacgt gacgctgatc gcgcccgagg cgggcggcga gctcgaggcg
     1981 ctcgccgccg aggggtcgat tcgctgggag cgacgcgagt acgccggccc ggacgacctc
     2041 gagggcgtct tcatggtcat cgcctgcacc gactcgaccg agaccaacat ccgcatctac
     2101 gaggacgccg agcggcgcgc gatgctcgtc aacatcgtcg acgtcccgcc gctgtgcaac
     2161 ttcatcctgc cggcgatcgt ccgcaccggc ccgctggcga tcgcgatctc caccgcgggc
     2221 gcctcgccag cgctcgccaa gcggatgaag cgcgaggtct cggagctgtt cggcgaggag
     2281 tacgcccgcc tggcgatcat gctcaacgac gtccgcggct gggccaaggg caccctcccc
     2341 acctaccagg atcgcaaggc cttcttcgag tcgatcgtca acggcacgcc ggacccgatc
     2401 gagctcctgc gcgccggcga cgaggccgcc gtccgcgacc tgatcgccgc ggcacagcgc
     2461 accgccgtcc cggcctgacc tttctagcct tcaattaaat gagatatatt tctcgtttat
     2521 gcgaacgtcc ccgccggacg cgctgccgat cttccgctcg gacctccagg cgcggctcct
     2581 cgccgcgctg ttcctgaacg acggcgcacc gctaaccacg cgtgagctcg ccgagcggac
     2641 agccgccaag ccggcgaccc tgcatcgaga gctcggccgg gtcgaggacg ccgggctgat
     2701 cacgcacgag agcgttgggc gcaccaagcg gtaccgcgca gcgacggagt cgccgctgca
     2761 ccgaccgttg cgcgagctcc tggagcgcac gctaggcgtc gagcctttgc tgcgccgcgg
     2821 gctcgctgag gtcgagggag tcgagcgggc cgcgatcttc ggctcctggg cggagggacg
     2881 cagcgacgcc ggcagcgaca tcgacctgct ggtgatcggc gacattgacc gcgacgccct
     2941 gctcgccgcg gtgcgcgagg ttgagcgcca ggtgcaccgc gagatcgacg tcaccgccta
     3001 ccgtcccgac gagttcgagc gccggcgcgg cgatgggtcg ggattcctgc gaacggttct
     3061 gcgcggaccg ctgatccagt tggtcggaga ggtcttgtga cgcgcgcgaa ggtccgggag
     3121 gtcgagcccg acagcgtcgc cgcagccgag ctgatcgccc aggcacgggc ccacctcgac
     3181 agcgcgcgcc tcgccggaat cgacgcccag tcggcctacg gcatctgcta ccaggcggcg
     3241 ctcaaggcga tggtcgccgc cctgctcgcc gacggacggc gggtgacctc cggggccggc
     3301 ggacacatcg tcacgatcca ggaagcggag gcccggctcg cgctggaccc gacggtgtcg
     3361 gtgcgaatcg atcacatgcg ccgcacccgc catcgagtct tctacgacgg ccacgaggtc
     3421 agcgagctcg agctcgaagg tgccctcgcg gatgcggagg ctgtggtggc cgccgcggcc
     3481 gaggcggtgg acgcgtgacg ggagatcggc gtggcggcgg ccgcggttcc accgaaatga
     3541 ccgccgcctc gccgagcggt gcggtcggga ccgaacgcca cgctgggacg gcgttggaac
     3601 tggaccgtct cgagcgccgc tacggcgacc gcgtcgcact gtccggcgtg acggtgcgcc
     3661 tcgcagtcgg cgagacgctg accgtgctgg gctccaacgg cgccggcaag acgaccctgc
     3721 tgcgcgtact ggcgacgctg ctacgtcctc acgacggaac cgcgcaggta ctcggatacg
     3781 agctgcccgg cgaggcgtgg agggtgcgtg gagcgattgg ctacctcggc cacgacccgc
     3841 tgctctaccg cgagctgacc gggcgcgaga accttcgcta tcacgcgcgc ttgcacggga
     3901 cggagggcgc gcgcgtggag gagcggctgg aggcggttgg gatggcgcgg cgcgccgacg
     3961 agccggtgcg cgagctgtcg aagggaatgg tgcagcggct cgcggtggcg cgcgcgaccc
     4021 tgcacgaccc gcagttgctg ctgctcgacg agccgagagc gaatctcgac ccggcggcgg
     4081 ccgaggcgct cgagccgctg atcgggcggg cgtcggggaa ggcgcgcgtg ctggtcaccc
     4141 acgatgtcga gggcgggctc gccgaggcgg atgtcgcgct gggattgcgt ggcggcaagc
     4201 aggtgttctg tagcccggcg gctgaagtcg acacggccac ggcgcggggt ctgtactcgt
     4261 gatcgccacg gcccgggcca tcctgcgcaa ggacctcgcg atcgagctgc ggacgaagga
     4321 gtccgtgccg gcgatgacgc tgttcacggt caccgtgttc gtgctgttcc acttcggcct
     4381 cgatcgtgac tcgctcgacg gcggcctcgc ctccggcgtg ctctgggtga cgctgctgct
     4441 cgcggcggtg ataggcgtgt cgcggctgtt cgcggtcgag cgcgagcagg gcgggatcga
     4501 cggcctgctg ctggcgccgg tcgaccgcac cgcgctgtgg ctcgccaaga ccgcggcgct
     4561 cttcctctac ctctgcctgc tcgagctggt ggccgtgccc gcgttcgccc tcttcctgct
     4621 cggccccgac ctgctgcccg cgctgcccgt cctgatcccg ctgttgctcg cggccaacgt
     4681 cggcctcgcg gcgatcggcg cgctcgtctc cgccctcgcc gccgagacac gcgcgcacga
     4741 gctgatcgtc ccgctgctgc tgctcccgct cgtcgtgccg ctgctgatcg cggccgcgtc
     4801 ggccaccgag ccgctgctgc gcggcgacca ggcgcccgag gacctgggcc gctggctggg
     4861 gctgctgagc ctctacgatg tggtcttcgt gctgatcgcc gtcgccgtgt tcgacttcct
     4921 cctggacgac taggtgtatt caaagggcct caagccgctg tcgatcgcca ccgtcgtcct
     4981 gatggcggcg tcgctcgcgc tcgtcttctt cttcgccccc aacgacgccg accagggctt
     5041 cgtccagaag atcttctacg tccacgtgcc gctggcgatc gtcgcgctct gcgggttcgt
     5101 cgccggcggg atcatggcga tcaagttcct gcgctcgggc gaccgctcgt gggacgcgcg
     5161 ctcctacgtc gcgatccaca tgtcgatcat cttcggcacc gcggtgctga tcaccggcgc
     5221 gatctgggcg aaggcggcgt ggggccactg gtgggtctgg gacgagccgg tgctcgtgtc
     5281 gttcctcgtc gtcttcctcc tctactgcgt ctactacccg ctgcgcttct cgatcgagga
     5341 ccccgaccgc caggcgcgct actcgtcggt gttcgcgatc gccgccggcg cgttcgtgcc
     5401 gatcaacttc gcggccgtcc ggatggccga ggccttcacc cacccgcgcg tcctgtcgca
     5461 gaccgggggc tcgatgccgg gcgacatgcg gctgacgttc ctcgtctcga tcgcggccgt
     5521 gacgctcctg ttcgtcacgc tgtggaagtt ggagctcacc ggcaagcacg cctcgatgca
     5581 gctcaagcgg ctgcggcgca agctcgaggc ggaggcgacg ggctgatgcc cgcgctcccg
     5641 ctcgacgagg ccggcaagta cgtcgccggc gcctacgtcg tcttcttcac cctgatcgtc
     5701 gtctacgtcg cgatcatcag cgccaagatc gcgcggatcg accgcgagct gaccgagctg
     5761 gtcgagatcg ccgagcgcga cgaggagcca gtcgcgtgag cggcgagctg ctggcgctcg
     5821 gcgtctcgca caagacggcg ccgctcgaac tgcgcgagcg gctcgcgctc accgagggcc
     5881 gtgcgaccgc cgtgctcgcc gagctgaccg cccacgagga gatccacgag gcggtcgcga
     5941 tctcgacctg caaccgcacc gagctctacc tcgtcgcgac cgacgccgtc gccgccgaga
     6001 gcgccgcgct cgccgtgctc gcccgccagg ccgagacgcg accgaccgag ctggccgact
     6061 cgctctacgc gctgcgcgac ctcgacgtcg cgcgccacct gttccaggtc gccgccggtc
     6121 tcgactcgat gatcgtcggc gagagcgaga tccagggcca ggtcaagcgc gcctacgagc
     6181 tcgcgctcgt cgaggggatg accggcccga tcgccaaccg cctcttccgc gacgcgctcg
     6241 cggcgggcaa gcgagtgcgg accgacacgg gcgtcggccg ctcgcgcgta tcggtggcga
     6301 cggtcgcggc cgagctcgcc tgtcgcagcc tcggcatcgt gggcggcgcg acaccgactg
     6361 tgacggacgc tcgctgcccc gccagcggcg gggacctgcc cgcacaccac ccttcgaccc
     6421 ccgacctctc cgaccgccgc gtgctcgtga tcggcgccgg cgagcagggc gagctgaccg
     6481 cgcaggcgct caaggaccgc ggcgcgcacg cggtcttcgt cgccaaccgc cactacgacc
     6541 gcgcgctcgg gctcgccgag cgctactcgg gctcggcggt cccgctcgac gagctgccga
     6601 acgagctggc gcaggccgac atcgtcgtca gctgcacctc gtcgccgcac cagctcgtgg
     6661 ggcgcgagga gctggcgctg gtgctcgagg gccgcgacga ccgtccgctg gtgatcatcg
     6721 acatcgccgt gccgcgcgac atcgactctg gcgtgcgcga gctgtcgggc gtcacgctct
     6781 acgacatgga cgacctcgag cacgaggtcg agcgcaacat ctccgtgcgc cgggccgagg
     6841 ccgagcgagc gctggcgctg gtcgagcgcg acgtcgagcg gttcgagggc tggctcgcga
     6901 gcctcgaggt gctgccgacg atccgcggcc tgcgcgagcg cgccgacgcg atcgtcgccc
     6961 aggtgctcgc cgagaacgcg ccgcgctggg agtctctgtc cgacgccgac cgcgagcgcg
     7021 tcgccgcgat gagccgtgcg ctcgtcgccc gtctgttcca cgagccgacg atgcgcctgc
     7081 gcgacggcgc cgaggacgag tcctcctaca tccacgtcgc ggtgctgcgc gagctgttcg
     7141 gcctcgatcc cgggcccgcg ccactcgacg aggacgagca gccctcggcc gaggtgaccc
     7201 agctggactc gcgccgccgc cggcgcggct gagcgtccgc cgtggcgacg cctctccgcg
     7261 tcgggacgcg tggcagcgcg ctcgcggtcg cccagtcgac agcggtcgcg cgcgatctcg
     7321 aggcgctcgg tgccgaggtt gagctggtca cgatccggac gtcgggcgac gagaccgcgc
     7381 ccaagcccga cacagaggac aaggcccgct tcgtcaagga gctcgagcag gcgctgctcg
     7441 atggcgagat cgacctcgcg gtccactccg ccaaggacgt cccgggcgag ctgccggacg
     7501 ggctggagat cgtcgccgtc cccgagcggg ccgacgcgcg cgacgccctc tgcctctcac
     7561 cgggtctgct cgatcgccgc ggccccgcgg agaccacggg cgcggagccg acaaccgcga
     7621 ccgaccgccc cgccgaccgg agtccctcga cttcgcccga cgcgtcggtc aaccacagcc
     7681 ccgccgccca accgctcggc gcgctgcccc ccggggcgac cgtcgggacc gccagcctgc
     7741 gccgacgcgc ggcgctgctc gcgctgcggc ccgacctcga tgtccgcgag ctgcgcggca
     7801 acgtcgacac gcgcctacgc cggctcgccg acggggagct cgacgcgatc gtcctggcga
     7861 ccgcgggact gatccgccta ggccgttccg acagcggcac cccactcccg gagctgacgc
     7921 tcgtccccgc cgccggccag gggtgcctcg cgctcgaggc ccgtgtcggt gacgactcca
     7981 cccgccgcct cgcctgccgc ctcaccgacc cgcgcgcgtt cgcctgcctg accgccgagc
     8041 gcacccttgt cacggcgatc gacgcgacct gtcacacgcc ggttggcgct cacgcgacga
     8101 tcgagaacca cacgatcctc cgcctgaccg ccttcgtcgg cactcccgac ggcgcgacct
     8161 ggatccgcga cgagatccgc ggcctcgccg agggccccca ggcggtcggg gccgatgcgg
     8221 cggaccgcct gcttgccgcc ggcgccgccg acatcctctc ctcggccgag gagacgccgc
     8281 tccgcgagct agccaggccg atcgccccgc gtcgcacgcc ccgctgtagc gttcggcaga
     8341 tggccgcggg catcgtctat ctggtgggcg ccggcccggg tgacccgggc ctgatgacgc
     8401 ggcggtcgct cgagctgatc gcggcggccg acgccatcct ctacgaccgg ctgatccccg
     8461 cggatgcgct cgacggcgcc cgtgcggact gcgagctgcg ctacgtcggc aaggcgccgg
     8521 gcgacgtggc gatgagccag gaggacatct gcgcgctgct ggtggagctg gggctggcag
     8581 gcaggcgggt cgtgcggctc aagggcggcg acccgttcgt cttcggccgc ggcggcgagg
     8641 aggccgaggc gctgcgcgcg gcgggcgtcc cgttcgaggt ggtgccgggg atcaccgcgg
     8701 gcgtcgccgc gccggcctac gcggggatac cggtgaccca ccgcgacgcg gcgacggcgg
     8761 tggcgttcgt caccgggcac gaggcgccgg agaagacgcg cgcgcgtggc gacggcgacg
     8821 ggccggagcg gccgcacagc gaggtcgact ggcaggggct ggcggccttc cccggaaccc
     8881 tggtcttcta catgggcgtg aagaacctgc cgctgatcgc cgaggcgctg atcgcggcgg
     8941 gccgtgaggc gagcgagccg gctgcggtgg tgcggcgcgg aacgctgccg gaccagcgca
     9001 cggtgaccgg cacgctcgcc gacatcgccg agcgtgtggc gcaggccggc ctgaaggcgc
     9061 ccgcgatcac ggtcgtcggg ccggcggtgg ggctgcgcga cacgctcgcc tggatcgagc
     9121 accggccgct gcacggcagg cgggtggcgg tcacgcgcgc ccgcgcccag gcgagcggcc
     9181 tggcagcgcg actgcgcgcg ctcggcgccg aggtcgtgga gacgccggcg atccggattc
     9241 agccacggcc cgtcgagggc gagatcgtcg aggcgatcga ggcgctgcgt ggggcgagca
     9301 tctctcccga gatccctgcg tcggctgcgc ccggggacga cgcggcgtac tcgctcgtct
     9361 gcttcacgag caccaacggc gtcggcctgt tcttcgaggc gctcgccgct cgggggttag
     9421 atgcccgcgc gctcgccggt tcgatcgtgg cggcgatcgg tccgggcaca gcgcgcgagc
     9481 tcgagcagca tggcgtgcgc gcggacgtcg tgccgccacg gtcggtcgcg gaggcgctcg
     9541 tcgaggcgct cgcggacgcg ccggtcgagg gacggcgggt gctcgtcgcc cgcgcgggcc
     9601 aggggcgcga cgtgctgccg gatgcgctgc gcgagcgcgg cgccgacgtc gatgtcgtcg
     9661 gcctctacga cacggtcgtc gagccgcttt cggacgagca gcgcgccgcg cttgcggatg
     9721 tcgactacgt cacgttcacc tccgcgtctt cggtgcggaa cctcgtggag tcgctcaccg
     9781 ccggccgtcg gccgccagat gagggcttcg catcgcctgg acggcccaaa cccacgtcgg
     9841 acgaagcggg cgggggcacc gtccgtgccg atgagggatc tgcatcgccg agactgcaca
     9901 agcccgcatc gggcgacggg ccgctcgggc gagcgcggat cgtctcgatc gggccggtga
     9961 cgagcgagca ggcgcgcgag ctcggcctgt cggtcgatgt agaggccgag cgacacgacg
    10021 tcgacggcct gatcgacgcg ctcgtcgtcg acgctgcagc caccccggtg accccatgac
    10081 gaacgccccg atcgtctccc tcctgaccga ctacggccgc gacgacgagt tcgtgggcgt
    10141 ctgtcacgga gtgatcctga agatcgcgcg cgacgcgcgg atcgtcgacg tcagccacga
    10201 gatcccacgc cacgacgtcc gccgcggggc gatcgtgctg cggaatgcgc tcccgtacgt
    10261 gccggccgac gtgcacgtcg cggtcgtcga cccgcaggtc ggcagcgagc ggcgggcgct
    10321 ggcggtccga tgcgaggacg gcacccagct cgtcggtccc gacaacggcg tgctgtcgct
    10381 ggcctgggac cgctgcggcg gggtcgacat cgcggtcgac gtgacgcgct cgccgcaccg
    10441 gctcgagccg gtctcggcga ccttccacgg gcgcgacctg ttcgtcccgg tcggcgccca
    10501 cctagccgcc ggcgcggaga tagccgacgc cggcgagccg gtcgacccgg ccacgctcga
    10561 ccggatcgcc ctaccgcacc cgctcttcga agacgacggc acgatcgtcg cccacgtcgt
    10621 cacgatcgac gcctacggca acgtcgcgct cgacctcacc cacgaccagc tcgccggcac
    10681 cggcctgatg ctcggggtga cggcggagat cgaggtcgac agcggcgacg gcggcgccgc
    10741 cgtcaagctc ggcggcggcc ggatcgcggc gccgttcgtc cacaccttcg ccgacgtcgg
    10801 tccgcacgag gcgatcctct acgaggacgc ctcgcgcgcc gtcgcgatcg cgatcaaccg
    10861 cggtgacgca gccgccgagc tcggcctccg gcccgacgac gaggtccggc tgcggccgcg
    10921 atgagcagcg gccgatgatc ggcgagccgc ggatccacca ccccgtcacc gactcgacga
    10981 acgagcgcgc acgcgagctg gccagtgaag gcgccccgca cggcacgctc gtcaccgccg
    11041 acgagcagac agccggccgc ggccgccagg ggcggacctg gaccgccgct cccggcgagg
    11101 cgctgctgat gtcggtgatc gtccgtgacc tcgagccgcg ccacgcgctg ctgccgttga
    11161 tggccgcgct ggcggtctgc gacgcggcgg aggccgtgag cgacgtgcgc tgcgagatca
    11221 agtggccgaa cgacgtctgg gtcgaccggc gcaagctgag cggcatcctg gtcgaggcgc
    11281 gccccgccgc cggctgggcc gtcgtcggga tcgggctcaa cacgggcgtg cgcgagttcc
    11341 ccgaggagct gcgcgagacg gcgaccagtc tccacctgcc cgagccggac gtggccctgc
    11401 cgccgctgct cgccgccctc gaccgttggc tcgccgccga ccaggccgac atcctcgccg
    11461 tctggcgccg gcgcgacgcg ctcgccggct cgcccgtccg ctggcaggac gggcacggga
    11521 ctgcgcgtgg ggtcgacgac agcggctcgc tgctcgtcga gacccccggc ggcgagctgc
    11581 tcgccctcgg cgctggcgag gtccatctgc gtcctccacc gggctgactg gccgcatcgt
    11641 tacgctgcgc cgatgtcgaa gctccggcgc gccaggctga agcagcggtt ggacccggtc
    11701 gccttcgact cgatcgcgac cgagacggac gagctgctcg agcggcgcct tcgcaaggcg
    11761 aagcggctga ccgacgtcgc cagccccgac gtgaacctca accccttcct gatgcttgcg
    11821 ctcgcgccgg cctacaacat cgcctcgccc ttcgaggctg cggagtacgt ccagctctcg
    11881 aagatgctgc acggcgactc gacagcgttc gggaggtatg tcgaggacaa gatccttccg
    11941 gtgttcggtg tgcgaccggt cgccgagaag aagcgaagcc cgaagctcta cagcccgatc
    12001 gacgccgagg tctcggtcga ggggaagcgc tacctgatga cgctcaaggc cgggccgtgg
    12061 acgatgaacc agtcccacgc gaacgagatg gtcaaccggt tccccgagat ccacgacaag
    12121 accggcgatg acatcgtcat cggcatcttc tacgggagcc ggagccggct gaacaacaag
    12181 ccgcgtctcg tggaggtgaa caccggcgac tacgtgacga cgctggtcgg caaggacttc
    12241 tgggagttcg tcaccggcgt caaggacgcc caccaggaga tcttccgcgc gatccgcgag
    12301 gcccagagcc gcttcgcggc ccggcacggc ggagagacgt tctacgagca gatgatcgag
    12361 gcgcggctgg cgctctcacg ctccatcagg gaggcgttcg gcctcggcgc ggaggacatc
    12421 gacatgtggg agcagctctt cgagggagcc ttctaggcgg ggaccgcgac gtccgcgcca
    12481 tcggcacgaa ggtaagcgtc accgtccttg taccagcggc gggtcctgac atgcgacttg
    12541 agatagacac gcctgaacgg ctcgagggcc gcgacaggct cgggcgaagc cgctcgcgcg
    12601 tcgcggatcg cctcgccggc gacctccgct agacggatcg gcacggcgtt gccgacttgc
    12661 gcgtactgct gctgcggagt gccagagaac ccccattcgt ccgggaagcc ctggacccgc
    12721 gcacactcac gcaccgtgag cgttcggacc tcgtcggggt ggcatagaga gctgctcgcg
    12781 tggttgggca tcgtcacgat cgtcgggcag ggcaggtccc aggacagcct tcgccaccag
    12841 ccggagcgcc cgcccttggc atggaaggct cggcccatcg actcgcgctg caggcgctcc
    12901 ggaagcgctc tccagttgcc gcccggcgga accttcccga ggtaggcctt cttcctcgca
    12961 ctgaagtcca tgatcaccgg cgcgtcctcc tccaacccgc gcagggcgtc cgcgagcgtc
    13021 tcatagggct ggaggccagg agccactcca tgggtgggct cagggaagcg caccaccttc
    13081 ccttccctgt tgccgaccag cagcacccgc tctcggagct gaggcgcgcc gtagttcacc
    13141 gcattgacct cgaagcagtc gacgcgatag gcggggtcag ccgcgcgcag gtcgtcgatc
    13201 catgccgcaa cgacggagcc cggctcctcg tcaagggcca atggagggcc gccgtggact
    13261 ggacgctcgg cgatcggccg gtggcgcagc gcggccgaga gcaggccgcg cacgttctcg
    13321 agcaggaaga acctcggctg gaactcctcg acccaccgca ggtaggacca gatcatcgtc
    13381 ccgcgcgggt cctggaccgt ccgccgcctg ccagcggtac tgaaggactg gcagggcggc
    13441 cctccgacga gcaggtccag ctctccgcgc ttcaggccca gttcgcggcg cgccgtgcgt
    13501 gggtcgagcg tcgcgaggtc ggcacgccgg accacggttc gagacgatcc gagaaggccc
    13561 cgctcaacgt tgtcgcgaag ggcctccacc gccatgggat cgaactcgac cgccagcagg
    13621 agctcgcagt cggcagctcg ctcgacgcct atgtcgaggc cacccgctcc cgtgaatagg
    13681 ctgatcgcgc gcatagtcct gccgacgaac gtctgttcgc tggcggagcc gagcttatat
    13741 gcctgggcgg acggtcaagc ggttggcccg gaccagccga ccgatgcgcg cagcagcagc
    13801 atgcacgtca tcgtgctccc agacgcggac gacggcccac ccctccgcgc tcaagacggc
    13861 gtcttgacgt cggtcgcgct cgacgttgcg gcgaagcttc gcggcccagt agcccgagtt
    13921 gctcttcggt acgcggccgt gttcggggca gccgtgccag aagcagccgt cgacgaagac
    13981 cgccacccgg gccagcgtga agacgatgtc cggacgacac ctgaacccgg cgatcggcgc
    14041 caacccaacg cgataccgga ggcccatctg atgcagccgc ctccgaagcg cgacctccgg
    14101 tgacgtgtcg gcgccgcggt tgccctgcat gacggctcgc accgccttgg agctcggcgg
    14161 tggtgccgga ggaagggtga ggtcatcggg acgggacaca gctcacagac gctcggccac
    14221 ctcgtcctct gcctcgggtc cgggctcggc agccttcgag ggcacgggct cggtgtccgc
    14281 actgccgttg gccgacgcgt cgtgctgctg ctcctgcgcc tccgccgcct tgtcaccatc
    14341 ggcggaccca tcgcccccgc ccttcttgcg ccgccgcccc gagcgcttgc ggcgcccctt
    14401 ggggaagttg cgcagcagct cacgggcgag cgccgacggc ttgcgcccca gccgcatccg
    14461 cgccccgtcg agcacctgct gcgcctccgg cgtgtgcgcc aacgccgccg ccagcagcgc
    14521 cgccgacagc cgccggtcct gcttcttcgt tgcgtgcaca agccgccgcg cgttgcgcgc
    14581 gtgcgccccg cccgacgagt tcaccagcag tcccaacagc cgcttggtct ccggccaccg
    14641 cgagaccgcg tactcctcgt gcacctcgcg cgagtactcg gccaggtgcc agatccagcg
    14701 ggcggcctgg tcgcgccggc ccatcggccg ctcggccatc gcctgcagca gcgtcttgac
    14761 gccctcgcgg cgctcgtcgc gctgccaggt cccgacgatc tcaaccgcga cgtcgaacgc
    14821 ctccgggtcc ttgatctggc cgatctcctg cagcgcgcgc acgcgcagct gcgagttgcg
    14881 gttgcgctgc aggcactcga tcaggaagcg ccgccgcagc tcgccggtcg agtcgaagtg
    14941 cagcatcgcc ttcgcccggc gccagttgtt cgacgccacg cgcgcgcccg cgagcgccgc
    15001 ccaggcgtgc tgctcgcccc atgacaggtg ctcgaccgcg atccgccaca gctcgcgctc
    15061 gaagacctgc gcgcgccgct cgtcgtcggc ggtcaccggc accacgcgcc gctcgacgcg
    15121 cagccgccgc ccacgcccgc gccgcaccgg cccgacgacg atcgcgccgc cgcggtagac
    15181 gtcgcggtcg agcagcgagt gcagcgtcac gaccgggtcg tcgttggtcg tcgccggatg
    15241 gacgaagtcg acgacgatcc ccgcctcctt gccctggtgg cggcgcgtca cgcggccgac
    15301 acgctgctgg tagatccgct tcgacgccgt cggcgcgagg tgcaggcaga ccgtcgcgcg
    15361 cggcgagttc cagccctcgg caagcagctg cgcgttgacc aggacgtcga ccttgccgag
    15421 ctcgtagtcg gagaggatcc gcgtcagctc gcgcttcggc gtctcgccgg agaccgcctt
    15481 cgccttgatc cccgccgcca cgaacgcgtc ggcgacgttg tgagcgtgcg ccaccccggc
    15541 cgagtacacc acccccggca gattcttgaa gcgcgtcttg tagaggtcgg cgatcgccag
    15601 gttgaacggc gtctggtcga gcagcgctgc gagctcctcc tggtcgaagt cctggtcgac
    15661 ctcgcccttg cgcagcggca ccttcgagat cgagcgcacg ccggcgcccg gcgggatccg
    15721 cacgcagcgc agcggcgcga tcacgccgcg ccgcgccgcc tgggcgagat cgaagcgcga
    15781 cgtctgggtc gggaagaggt cggtgacgtg gcgggcgatc agcgcgccgg tcgccgtcat
    15841 gccgacgaac accgggccgt cccactggcg gatcgacgcg ctcgtcttct caccgagcgc
    15901 ggtgtgcgcc tcgtcgcaga tcacgacggt gtagtcgtcg gagatcttgc cggcgttgcg
    15961 cacgtaccac tggtaggtct cgacggtcac cggcccctcg acgcgcgcct tgtccctgcc
    16021 gagtagcggc ggcgagatcc gcttcttgta gccgcggtcg gtcagctcgc cgttgaactg
    16081 gtcgacgagg ttgcggcggt gggtgaggat cagcacgccg ccggtgcgcg acgcctcgac
    16141 gaagccgagc gcggcgacgg tcttgccggc gccggtcgcg tgctcgaacc agaagcggcg
    16201 ggcggcgccg gggtcgtcct cgacggagtc ggcggcggcc tcctcctcgg cctcctcgtt
    16261 cgcgtcccag tcgagcggct cgtcgtcgtc ggagtcgtcc tcatcgtcgt caccgtcgat
    16321 cgctatctcg ccggacggca gctcctcgag cgcggctggg gctaccttgc cgttgctctt
    16381 ggactggacc tccgcggtca gcgcgatcaa cgtccccgac agcgcatcga cctggtgcgc
    16441 cgacagcacc gtcccgtcga ccagcttcgg ctcgtcgtcg gcgagcacgc gctccagccc
    16501 caggaccagc gaccagcgcc gcttccactc cgccgacggg cgctcggcgc cgtcggcgat
    16561 ctcggccagc gcgtcatcga gggccgcgcg acgcaccgtc ccgggcgcga gcgcggcgtc
    16621 gcggtcctcg ccctcgagga cgaacggctc gcgcgcgaat gcctcggcgc gctctagatc
    16681 gtcgccggat accggcgccg ttgcggcaac ggaagtgctc tcagccatat gcggccttcg
    16741 gttcttgtac gagaaaggcg acggaccgag ccggctccgc gcggagcgtt gatcttcgcg
    16801 ggaggcagtg cggccgtccg gggcacaccg ctctggcgac tgcctgcgag cggtgctctc
    16861 agcgcatccc aggcgctgac tcgataccta ttacagcaca gcatgcggcg tttccttcca
    16921 gtactggtgt ccttcgtcac agtcgtgggc gtcgcgacgg gctgcggggg cgacgatccg
    16981 cagagcgcgg cgccctcgca ggaggcaacg cgcgacgcac tgaagggcgc tcccgcaccg
    17041 ctcgcggcgg tccatgccca ggccaacgag ctgctcgacg gcggtccgaa ggctttcaag
    17101 cagcggctcg ccgagctgcg cggccatccg gtcgtcgtca acaagtgggg ctcctggtgc
    17161 gggccctgtc gcgaggagtt cccctacttc cagcgccagg cggtcgagca cggcaagcgc
    17221 gtcgccttcc tcggcgtcga cgtgcaggag tcgaaggccg agggtcgcga gctgctcgag
    17281 gagttcccgg tcagctaccc ctcctactgg gacccgcgcc tgaccatctc cgccgtcttc
    17341 aacggcgtcg ccgccacacc cgcgaccgcc ttctacgact ccaagggcaa gctcgcctac
    17401 ctcaagcagg gcccctacct cagcgaccgg gacctcgccg aggacatcca gcgctacgct
    17461 cgctgacctc atgcaggagc tgcgcatcga cccgctgagc gggctgcgcg tggcgatcgg
    17521 ggagctcgac gcgagcgccc cgcaggcgcc aggcgagacc gccgccgaga ccgacgaccc
    17581 gctcgccggc ggacgcggcc agccggagat gttcgctgtc gggcgggcgg gggcggggac
    17641 cgtcgagacg atcgacagcg tgccggggcc ggtcggctcg ctgctcgagc tgggggcgga
    17701 tggcctcgag gagacgatgg ggctgtggcg cgagcggatg cgcgcacacg acggcgtggc
    17761 atgccggcac ctgatcgtca gcgagggcga ggcggcgggc gcctcgctgc cgcactccca
    17821 cgcgcagctg ttcgcgctgc cgttcgtgcc ggcggcgctc gcgcgcgagc gtgagcggtt
    17881 caccgcctac caccagcgga cccagggacg gaacctgctc gaggatctcc tgaccgagga
    17941 ggtccggcgc ggcgaccggc tcgtcgtcgt cgaccgcgag gcgatcgcga tctgcccgtt
    18001 cgcctccgcg gcgcctttcc agctgcagct gatcccacgc cggcccgcgg cgcgcttcga
    18061 ggacgacgga ccgctcggcg cggcggcgct gcacggcgcg ctcgagcgtc tgtccgcggt
    18121 gctcggtgag ctgccgccgt tcaacctctg ggtgcggacg gcgccgagcg gcgccgagtc
    18181 gttctgctgg cggatcgacc tgctgccgcg cgtagccccg cccggcggac tcgagctcgg
    18241 caccggcgtg cggctcaacg cggtctcgcc ggaggacgcg gcggagcggc tgcgcgcggc
    18301 ggccgcggcg tgatcccgat cgccctgacg atcgccgccg cgacggcggc gggcttcggg
    18361 gtcgagcacc gcttgggcga gctgcgctcc cagcacgtcg ccgagctcgt gacgcggctg
    18421 atgctctgga tcctgctgcc gccgatcgtc ttcttcaaca tcgccgacct ccacctgacc
    18481 gccggcgtcg gcctcggcgt gatcttcggc tgggtggggc tcggcgtcgc gatgctcgcg
    18541 gcctacgcga tcggcaagtg ggtgctgcac ctctcgcgcg cgtcgaccgg caccctgatg
    18601 ctcgcggccg gcctcagcaa caccggctac ctcggcctgc cgttcaccgc cgcgctgttc
    18661 ggcttcgacg acgtcggcga cgcgatcgtc ttcgacatcc tcgtgtcggg aatcgcgctg
    18721 ctgaccgtcg gattcggtat cggcgcggcg ttcggcacgg tcgcggaggc gcggcgcgac
    18781 cgaatcgtct ccttcttcac gcgcaacccg ggcatctggg cgggcgcggc cgccgtgttc
    18841 gcccccgcgg cactcgcgcc cgagtgggcc gtcgacgcgt cacggatcgc cgtcttcgcg
    18901 atgctgccgc tcggcttctt cgtcgtcggc atcgcgctcg cgagcgaggc cgaggagggc
    18961 gcgctccggt tccccccgcc gctgaccgcg cccgtcggcg tcgcgctcgg cctgaagctg
    19021 ctgctcttcc ccgcgatcgt cttcggcctc tcgcgcctga tcatcgacgt gcccgactcc
    19081 tactactccc aggccgcgat ggccagcggc atcaacaccg tcctgatctc ccacacctac
    19141 ggcatggacc gctcgatcgc cggcagcgcg atcgcctggt cgaccgcgat cgtggtggcg
    19201 acggggctgg tggtcgcgct cctatgagcg ttagacaatt agacgtctaa acatctagac
    19261 gatcatatcc tatatagttc cggtcgtgat ctatcgaacc ccgaagatcg acggcggcct
    19321 ggagcgacgc ctgctcgagc tcgacgacct ccggacccga ctcgggcagg agaccgaacg
    19381 cccgctgcgt tggataggga ccctcaggcg cctggtcaaa gcaaccaccg tggagagctc
    19441 cacctcgatc gagggcttct cggtctcgcc cgagaaggca gtgtcgctcg tcagtggcga
    19501 cgaccgcccg cgcgcaggcg acgaggacca gatggcggtg gccagctacg cccgggcgat
    19561 gcgtcatgtg gccgccatgg cagccgatcc acgcttccag tggtcggagc gagtgctgct
    19621 cgacctccac ttcgacgcct gcgagttcca aggcgacaag gacccagggc agtggcgaag
    19681 agggccgatc caagtgacgg gcgccggcgg acgaatcgcc taccgcgggc ccgatggcgc
    19741 ggaggtgccg gacctgatgg ccgaggccgt cgcctggctg cagcgcgggg acctcgaggc
    19801 gccggcaatc gtgcgcgcgg ctatggcaca tctccacgtc gccgccatcc atcccttccg
    19861 cgacggcaac ggacgggtcg cgcggatcgc gcagtcgctc gtcctagcgc gcgaggggct
    19921 ggtgtcgtac gagttcgcct cgatcgagga gtacctcggc gagcggaccc aggactacta
    19981 cgacgtgctc gagcgcaccc agggcgggag ctaccagccc gagcgcagcg cgcgggagtg
    20041 ggtcgagttc tgcgtcgagg cgcacatcga ccaggcacag cggcgcctgg gccagatcgc
    20101 cgccgccggc gagcgctgga gcaggctcga ggccatagcc gagagccgcg actggccgga
    20161 gcggctgatc gtcgcgctcg agcagggatt ggtcggtggc gcggaccgcg ccacctatgc
    20221 gcgcgaagcc gacgtatcgc cggccacggc tagcgcggat ctgcgacggc tggtcgacgc
    20281 cggcctgttg gatcagcgcg gacgcggccc cagcagccgt tacctcgcca gcgacgcgct
    20341 gcgccgcgag atcggctgac ggccgctgct ccggtagcgt ccgccgccgc gtgcccccct
    20401 ccgagcgacc gatcccgatg tacatcgccg agccgccgca ggagagcgcg ccgtacgggc
    20461 gctgggagga gacgctgcgg tcgcacttca tggtcgcggc cggggcgatc gacaccgagg
    20521 aggagatcgg cgagccgggc gcgctggcgt ggttcccgga gcgaacctgg ggcggccgga
    20581 ccttcgtgcc gtgcaccgcg ccgacctccg agggcttcga gctgttcggc tacgtctcct
    20641 acacgcgcga gcacgagggc gccgaggcga ccgacttcgc agccgtcgcc gactacaccg
    20701 acgagaccgc cgaggccaat cccgactgga ccctcgacct ctccgacgag gagatcggcc
    20761 actggcgcgg gcccgagggc cggcgcgggg tggtgacgct ggtctggggc gtcgcgctgg
    20821 tgcccgaggg cgccgtcgca accaccgagc tcggcccgac cacgaccgac cagtgcatcc
    20881 tcgccgacaa ccgcttcacc ctgatctcgc tcgacgccta caccggcgac tacgtcgagg
    20941 tccggctcta cgccaagcgc ggcgcggagc tggcgcgcga gtcgctgtac gaggacgagt
    21001 agcggggctg gccggccggt tgggcgcggc cgggcggcgg ctgtgcctcc cggagggcga
    21061 tcggcccgac gacgtgctcg atcgacgggt cgtcctcgtg ctccggcagt ccggtgacgc
    21121 cgaagcacgt gatcgtcagc ttccgatccc cgtcgacgtc gacgcggagg aagctcttga
    21181 agaacggcgg cgcggtcgcc tcggcgagct ccgacatgaa gcgcttgaac ggctgcgcga
    21241 agtaggtgac cttcgcgagc agctcggtgc ggctgcgccg ctcggggtcg ccgggcgtgg
    21301 cgcgcaccgg ctgcgagatc ccgagccgct tcgcgaccca gcgcgcaacc tcgtccgcgt
    21361 cgacgtaggg ccggaagagc gacagcgaca gcagcgccgg cagcgcgcgc aggttggcga
    21421 ggatcagcgc acaggcgagc agcgaccaga tcgccagcga cgcccagagc acgccggccg
    21481 gcctgagtgt tggagtgctc gtgatccgcc tgcccgcgct cctcctcgtc gtgctctccc
    21541 tgctcctggc cgccgacggc atgacggccg cca
//
LOCUS       DATABV010000157         9338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1977355, whole genome shotgun sequence.
ACCESSION   DATABV010000157 DATABV010000000
VERSION     DATABV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977355"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VHF88_06975"
     CDS             complement(<1..411)
                     /locus_tag="VHF88_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2161547.1"
                     /translation="MTGSRGPSAPELLAVLDRERPQPLHSQLEQALRDHIRAGRLAPG
                     TRLPSTRRLAADLGVARGVVSEAYAQLGAEGWLVGRRGGGTTVADRAVATPGGPDPAT
                     AGHERERIKFDFGVGVPDLSAFPRRRWLAALRAAL"
     gene            456..1124
                     /locus_tag="VHF88_06980"
     CDS             456..1124
                     /locus_tag="VHF88_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2161548.1"
                     /translation="MSDSVESSASAPSDRARVRRVPKRAVYERETVAAILDAARVSHL
                     GTVRDGAPVVVPMLHARVGDLVYLHGSSASVSVRGLRDGLPACLTATLIDGLVLARSA
                     FHHSANYRSVVVHGSAVLVEDPDEKLAALEAFTEKVLPGRWREVRPPNPLELKATAVL
                     ALPLEESSAKVRDGDPVDDEEDYELDCWAGVVPLETIVGTPVPDARLRPGTPLSQAVS
                     RLAG"
     gene            1202..2626
                     /locus_tag="VHF88_06985"
     CDS             1202..2626
                     /locus_tag="VHF88_06985"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX2161549.1"
                     /translation="MTLRELMGNGAPEVEVSGLAYSSRSVSPGTLFFCVPGFKADGHD
                     FAPDAVERGAAALVCERPLGLGVPEVVVDDVRAAMGPVAARFHDDPTARLRVVGITGT
                     NGKTTTAFLVRDVLEAAGVRTGLLGTVKQVVGGVEEPVVHTTPEAIDLQATFRRMVDG
                     GDAACAMEVSSHALELGRAAGIHFACRVFTNLTQDHLDFHPDMEAYFLAKRRLFDGPG
                     PAIVNVDDAYGRRLAEELDAPTTYAIDSAADFRACDVGFDVKGARFTAETPDGPLEVT
                     TSLPGLFNVSNALAALATARALGVDPEACRTGLARAGRVPGRLEPVDEGQRFGVFVDF
                     AHTPDSLDNVLRAARDLTERRLHVVFGAGGDRDRAKRPLMGAAAAARADRVIVTSDNP
                     RSEDPEAIVAEVLEGTGPGAEREPDRRKAIELAVATAAPGDVVVIAGKGHEQGQEFEG
                     GRKEPFDDREVARGALRALMAETA"
     gene            2623..4005
                     /gene="murF"
                     /locus_tag="VHF88_06990"
     CDS             2623..4005
                     /gene="murF"
                     /locus_tag="VHF88_06990"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX2161550.1"
                     /translation="MNGRTTGWVVEASGGRLLAGDPAAAGPSGVVVDTRLAGPGDLFV
                     GLPGERSDGGLHAPDALAAGAWGVLVAPEHGERAVHAAPAGTAVIAAPSGGVDGGPGA
                     RPGPLAALQSLARAWRRELGCKVVGVTGSTGKTSTKDILAALLEPHLSTHANSENLNT
                     EIGLPLTILDAPPGTEALVLEMAMRGEGQIAELAAIAEPDVGVIVNVGPVHLELLGTV
                     ERVAAAKAELIRDLPAGATCVVPASEPLLERHLRDDLDTVTFGPSGDVMLLSFEVHGD
                     RRGGVAEIEARGQRVLLDLPYAEPHNLLNTLAAVGAAQAIGTRPNGAVEVAFSALRGE
                     IVELAGGVTVVNDCYNANPMSMRAALKHLAETPAQRRIAVLGTMAELGGASEAFHREI
                     GEEASALGIDVLITVGERALPYGQGYDGETYAVPTPEEAGALLEEIVLPGDRVLVKGS
                     RSAGLERVLA"
     gene            4008..4982
                     /gene="mraY"
                     /locus_tag="VHF88_06995"
     CDS             4008..4982
                     /gene="mraY"
                     /locus_tag="VHF88_06995"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX2161551.1"
                     /translation="MGEILIGGMASLLICIFLGPKFIEWLRAKEFGQHIREDGPANHH
                     EKAGTPTMGGVVIFIGVSVPFLILTDYRAISLVVLAVALASAGLGFADDWAKIQKRRS
                     LGINGRTKLAIQALIAVGLWLAATEYVGLNDTLRLRIVDASIDLGYLYPGLILLVLMG
                     TTNGVNLTDGLDGLAAGCSAIVLLAFTAMTFITGGPAQADLALLCACLVGACVGFLWF
                     NSFPASIFMGDTGSLGLGGAIAALAVMTQTEVLLLIIGGIFVIEALSVVIQVFAFQRF
                     RRRVFLMAPLHHHFEMLAWSETKIILRFWIIAAICSAIGFTLYQQSIP"
     gene            5018..6289
                     /gene="murD"
                     /locus_tag="VHF88_07000"
     CDS             5018..6289
                     /gene="murD"
                     /locus_tag="VHF88_07000"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX2161552.1"
                     /translation="MGLARSGLAAARMLTVRGEEVVGVDAGSPVVNLPMLETHLGADG
                     VELLVERGICTVVKSPGVPNDAPVVVAAREQGIEVVGELELAWRLLPNRFVAVTGTNG
                     KTTTVELLGAIWRAAELPVAVAGNVGTPLSSLVGLLDPGTTVICEASSFQLEDSSAFA
                     PEVAVLLNVEEDHLDRHGDLASYRAAKERIFANQRPEDWAVAPPDLVPAGAGSWVGFG
                     DGADAEVEDGWIAFRGERLLPVADLRIRGAHNLDNALAATAAALAGGDVPPGAVAQAL
                     RSFPGVEHRLEEVATVAGVLYVNDSKATNPASALRGLQPFEGGVHAILGGSLKGGDFT
                     ALRGEVGARCRACYLIGEAADRLAADLDGTVPLCRSGTLERAVADARAATEPGDVVLL
                     SPACASFDAFANYEQRGARFKDIVAGLEREE"
     gene            6320..7600
                     /gene="ftsW"
                     /locus_tag="VHF88_07005"
     CDS             6320..7600
                     /gene="ftsW"
                     /locus_tag="VHF88_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX2161553.1"
                     /translation="MAKRGTRPIEYSILYTATLCMLAVGAVMVYSASSAESLLDGSGD
                     PSMYLKRYVAFGLVGLGVMLLAARHGLALVRQLTPSLLGASFFLTIAVMLPGVGVTIN
                     GATRWLAAGPLQFQPSELLKVSLVLYAAQLLATRPRSVETLEGIAKPLLIVVGAAFLL
                     LMKQPDMGTTMVICFAIGSLLIVAGTPMRLIGMLAGGLAVLAFVLAIVEPYRMERMTA
                     FLNPFADAGDTGFQAVQGLTAIGSGGLFGVGLGESVQKIFYLPEAHTDMILAIIGEEL
                     GLVGIAGVAGLYVMIGYAGLRAAKAARDRYSKLVAAGITSLILCQATLNFFAVLGLAP
                     LTGVPLPFISYGSTNLIVLLAGMGLLLNVAATGGVAARGAKPKRAGAADADRSSAVRL
                     RAIDGGGADEGRDRRRRDGGARRAGAGSRRRTAG"
     gene            7521..8600
                     /locus_tag="VHF88_07010"
     CDS             7521..8600
                     /locus_tag="VHF88_07010"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEX2161554.1"
                     /translation="MKVAIAAGGTAGHVVPALAVADALRAEGAAVEFLGGERAEAELV
                     PAAGYPLHPLRVAGIDRRNPLRALRALALAVRATARARRLLREIGADVVIGGGGYVAG
                     PVGLAARTLRLPLVLTEADSHLGIANRLLAPAAARVFLAFPLAGCDGDRYAVVGRPIP
                     RGTLGADRIAARARFGIGADERCLLVFGGSLGARTINDATVEAFGAAAPCAVLHACGR
                     RDHEDLADRLDKLGSPAHYRLHDYIQPFADALAAADLAVARSGGSVFEVAAAGVPSIL
                     VPYPHATADHQTLNARHFAAGGAAAVVPDDELDAARLSREVGTLLADAGRLDEMGRAA
                     RALARPDAAETIAREVLELGKAATR"
     gene            8603..>9338
                     /locus_tag="VHF88_07015"
     CDS             8603..>9338
                     /locus_tag="VHF88_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX2161555.1"
                     /translation="MEPPFADRRLHFVGIGGAGMSGLALVAAELGATVSGSDRAASSY
                     TARLRAAGIEPALGHDAANLPAGAEVVVSTAIPADNPELAAARAAGAKVIHRGQLLGE
                     LTRLKRSIAIAGTHGKTTTCGMVVHVLRATGRDPAFVIGGELSSAGTNAAWGPGDWIV
                     AEADESDRSFLELQRDVAVVTNIELDHHATYTSLTDLQSTFAQFAAPANLTIAWDHID
                     PATLAPNTPTDAHASIPDAQVAAGDEG"
BASE COUNT         1178 a         3409 c         3408 g         1343 t
ORIGIN      
        1 cagtgcggcg cgcagcgccg ccagccagcg ccggcggggg aacgcggaga ggtccgggac
       61 gccgacgccg aagtcgaact tgatgcgctc gcgctcgtgg cccgctgtcg ctggatcggg
      121 gccgcccggc gtggcgacgg cgcggtcggc gacggtggtg ccgccgccgc ggcggccgac
      181 gagccagccc tccgcgccga gctgggcgta ggcctcgctg acgacgccgc gtgcgacgcc
      241 gaggtccgcg gctagccgcc gcgtggaggg gagtcgagtg ccgggcgcga gccggccggc
      301 gcggatgtgg tcgcgcaatg cctgctcgag ctgggagtgg agcggctgcg ggcgctcgcg
      361 gtcgagtacg gcgagcagct cgggcgcact tggcccgcga gaaccggtca tgaatgggcc
      421 tttatagcgg gccaatcgcg gcctagggtc gtgacatgag cgattccgtg gagtccagcg
      481 cctcggctcc ctcagatcgc gcccgtgtgc ggcgggtgcc gaagcgcgcg gtctacgagc
      541 gcgagaccgt cgcggcgatc ctcgacgcgg cgcgtgtcag ccatctcggg acggtccgcg
      601 acggcgcgcc ggtcgtcgtg ccgatgctcc acgcccgcgt cggcgatctc gtctacctgc
      661 acggctcgtc ggcgagcgtc tcggtgcgcg ggctgcgcga cgggttgccg gcgtgtctta
      721 cggccacgct gatcgacggg ctggtgctgg cgcgctcggc gttccaccac tccgccaact
      781 acaggtcggt cgtcgtccac ggcagcgccg tgctggttga ggatcccgac gagaagctgg
      841 cggcgctcga ggcgttcacg gagaaggtgc tgccgggccg ctggcgcgag gtgcggcccc
      901 cgaatccgct cgagttgaag gccaccgcgg tgcttgccct gccgctcgag gagtcctccg
      961 cgaaggtgcg cgacggcgac ccggtcgacg acgaggagga ctacgagctc gactgctggg
     1021 ccggtgtcgt gccgctcgag acgatcgtgg ggacgccggt ccccgacgcc cggctgcgcc
     1081 cgggcacccc cctgtcgcag gcggtatcgc ggctcgccgg ctgagtcgcc ggttgcgttg
     1141 agaccggggc ttccgaacgc aagccgagac gcccggaggc gactctagac ttgcgggctc
     1201 catgaccctg cgggaactga tggggaatgg ggcgcctgag gtagaggtct cggggctcgc
     1261 ctattcgagt cggagcgtct ccccgggaac gctcttcttc tgtgtgcccg ggttcaaggc
     1321 cgacgggcac gacttcgcgc cggacgccgt cgagcgcggg gctgcggcgc tggtctgcga
     1381 gcggccgctc gggctcggcg ttccggaggt ggtcgtcgac gacgtgcggg cggcgatggg
     1441 gccggtggcg gcgcgcttcc acgacgatcc gacggcgcgg ctgcgcgtcg tcgggatcac
     1501 cggcaccaac ggcaagacga cgacggcgtt cctggtccgc gacgtgctcg aggcggcggg
     1561 cgtccggacc ggcctgttgg ggacggtcaa gcaggtcgtc ggcggcgtcg aggagcctgt
     1621 cgtccacacg acgccggagg cgatcgacct gcaggcgacg ttccggcgga tggtcgacgg
     1681 gggcgacgcg gcctgcgcca tggaggtctc ctcgcacgcg ctcgagctcg gtcgcgccgc
     1741 agggatccac ttcgcttgca gggtgttcac gaacctgacc caggaccacc tcgacttcca
     1801 ccccgacatg gaggcttact tcctcgcgaa gcgcaggttg ttcgatgggc cggggccggc
     1861 aatcgtgaac gtcgacgacg cctacgggcg gcggttggcg gaggagctcg acgccccgac
     1921 gacgtacgcg atcgactccg ccgcggactt ccgggcgtgc gacgtcggct tcgatgtcaa
     1981 gggcgcccgc ttcaccgccg agacgccgga cgggccgctc gaggtcacga cgtcgctgcc
     2041 ggggctgttc aacgtctcca acgcgctcgc ggcgctcgcg acggcgcggg cgctcggcgt
     2101 ggatcctgag gcctgccgta ccgggctcgc gcgggcgggg cgcgtgccgg gtcggctcga
     2161 gcccgtcgac gagggtcagc ggttcggcgt cttcgtcgac ttcgcgcaca cgccggactc
     2221 gctcgacaac gtcctgcgcg cggcgcgcga cctgacggag cggcggctgc acgtcgtctt
     2281 cggcgccggc ggcgaccgcg accgcgccaa gcggccgctg atgggcgcgg cggccgcggc
     2341 ccgcgcggac cgggtgatcg tgacctccga caacccgcgc tcggaggatc cagaggcgat
     2401 cgtggccgag gtgctcgagg ggaccgggcc cggcgccgag cgcgagcccg atcggcgcaa
     2461 ggcgatcgag ctggcggtcg cgacggccgc gccgggcgac gtggtggtga tcgcgggcaa
     2521 ggggcacgag cagggccagg agttcgaggg cggccgcaag gagccgttcg acgaccgcga
     2581 ggtggcgcgc ggcgcgctgc gcgcgctgat ggcggagacg gcatgaacgg caggacgacc
     2641 ggctgggtcg tagaggccag cggcggcagg ctgctcgccg gcgatccggc ggcggcgggc
     2701 ccctcgggcg tcgtcgtcga cacgcgtctc gccggccccg gcgacctctt cgtcggcctg
     2761 cccggcgagc gcagcgacgg cggcctgcac gcacccgacg cgctggcagc cggcgcctgg
     2821 ggcgtcctcg tcgcgcccga gcacggcgag cgggcggtcc acgcggcacc cgccggcacg
     2881 gcggtgatcg cggcgcccag cggtggcgtg gatggcggcc ctggcgcccg tccggggccg
     2941 ctcgccgcgc tccagtcgct cgcccgcgcc tggcgccgcg agctcggctg caaggtcgtc
     3001 ggcgtcaccg ggtcgaccgg caagacgtcg accaaggaca tcctcgccgc gctgctcgag
     3061 ccgcacctga gcacccatgc caacagcgag aacctcaaca ccgagatcgg gctgccgctg
     3121 acgatccttg acgctcctcc gggcactgag gcactggtgc tcgagatggc gatgcgcggc
     3181 gagggccaga tcgccgagct ggccgcgatc gccgagcccg acgtcggggt gatcgtcaac
     3241 gtcgggccgg tccacctcga gctgctcggc accgtcgagc gcgtcgccgc cgccaaggcc
     3301 gagctgatcc gcgacctgcc cgccggcgcg acctgcgtcg tcccggcctc cgagccgctg
     3361 ctcgagcggc atctgcgcga cgacctcgac accgtcacct tcgggccgag cggcgacgtg
     3421 atgctgctgt ccttcgaggt tcatggcgac cgccggggcg gcgtcgcgga gatcgaggcg
     3481 cgcggtcagc gcgtgctgct cgacctgccc tacgccgagc cgcacaacct gctcaacacg
     3541 ctcgctgcgg tcggcgcggc ccaggcgatc ggcacgcgac cgaacggcgc ggtcgaggtc
     3601 gcattcagcg cgctgcgcgg cgagatcgtg gagctggccg gcggcgtgac ggtcgtcaac
     3661 gactgctaca acgccaatcc gatgtcgatg cgggcggccc tcaagcacct cgcggagacc
     3721 cctgcccagc gacgcatcgc ggtgctcggc acgatggccg agctcggcgg ggcctccgag
     3781 gcgttccacc gcgagatcgg cgaggaggcg tccgcgctcg gcatcgatgt gctgatcacg
     3841 gtcggcgagc gggcgctgcc ctacggccag ggctacgacg gcgagaccta cgcggtcccg
     3901 acgccggagg aggccggcgc gctgctcgag gagatcgtgc tccccggcga ccgcgtgctg
     3961 gtcaagggct cgcgctcggc cggtctcgag agggtgctcg cctaggcatg ggcgagatcc
     4021 tgatcggggg gatggcctcc ctgctgatct gcatcttcct cgggccgaag ttcatcgagt
     4081 ggctgcgtgc gaaggagttc ggccagcaca tccgcgagga cggacccgcg aaccaccacg
     4141 agaaggccgg cacgccgacg atgggcggcg tagtgatctt catcggggtc agcgtcccct
     4201 tcctgatcct caccgactac cgcgcgatca gcctcgtcgt gctcgccgtg gcgctcgcct
     4261 ccgccggcct cggtttcgcc gacgactggg cgaagatcca gaagcgccgc tcgctcggga
     4321 tcaacggccg caccaagctg gcgatccagg cgctgatcgc ggtcgggctc tggttggcgg
     4381 ccaccgagta cgtcgggctc aacgacacgc tgcgactgcg gatcgtcgac gcctcgatcg
     4441 atctcggcta cctctatccg ggcctgatcc tgctcgtcct gatggggacg accaacggcg
     4501 tcaacctcac cgacggcctc gacggcctcg cggccggctg cagcgcgatc gtcctgctcg
     4561 cgttcacggc gatgacgttc atcaccggcg ggccggcgca ggccgacctc gcgctgcttt
     4621 gcgcctgcct ggtcggcgcc tgcgtcggct ttctctggtt caactcgttc ccggcgtcga
     4681 tcttcatggg cgacaccggc tcgctcgggc tcggcggggc gatcgccgcg ctggcggtga
     4741 tgacgcagac cgaggtgctg ctgctgatca tcggcgggat cttcgtgatc gaggcgctct
     4801 cggtcgtgat ccaggtcttc gccttccagc gcttccgccg ccgcgtcttc ctgatggctc
     4861 cactgcacca tcacttcgag atgctggcct ggtcggagac caagatcatc ctgcgcttct
     4921 ggatcatcgc cgcgatctgc agcgcgatcg gcttcaccct gtaccagcag tcgatcccgt
     4981 gagccggccg ccgctgccgc ccggaccctt cctggtcgtg gggctcgccc gatcgggcct
     5041 cgccgcggcg cggatgctga cggtgcgcgg ggaggaggtc gtcggggtcg acgcgggcag
     5101 cccggtcgtg aacctgccga tgctcgagac gcacttgggc gccgacggcg tcgagctgct
     5161 cgtcgagcgc ggcatctgca cggtcgtcaa gagccccggc gtgccgaacg acgccccggt
     5221 ggtggtcgcg gcgcgcgagc aggggatcga ggtcgtcggc gagctcgagc tcgcctggcg
     5281 gctgctgccg aaccgcttcg tcgcggtcac cggcaccaac ggcaagacga cgaccgtcga
     5341 gctgctcggg gcgatctggc gcgccgcgga gctgccggtc gccgtggccg gcaacgtcgg
     5401 cacgccgctg tcgtcgctgg tcgggctgct cgacccgggg acgaccgtga tctgcgaggc
     5461 gtcgagcttc cagctcgaag acagctccgc tttcgctcct gaggtcgcgg tgctgctcaa
     5521 cgtcgaggag gaccacctcg accggcacgg cgacctcgcg tcctaccggg cggcgaagga
     5581 gcggatcttc gccaaccagc gaccggagga ctgggcggtg gcgccgcccg acctggtgcc
     5641 ggccggcgcc ggctcctggg tcggcttcgg cgacggcgcc gacgccgagg tcgaggacgg
     5701 ctggatcgcg ttccggggcg agcgtctgct gccggtcgcc gatctgcgga tccgcggagc
     5761 gcacaacctc gacaacgcgc tagctgcgac cgccgccgcg ctggccggcg gcgacgtgcc
     5821 gcccggggcg gtcgcgcagg cgttgcgcag cttcccggga gtcgagcacc ggctggagga
     5881 ggtcgcgacc gtcgccggag tgctctacgt caacgactcg aaggcgacca accccgcgtc
     5941 ggcgctgcgt ggcctgcagc cgttcgaggg cggcgtgcac gcgatcctcg gcggcagcct
     6001 gaagggcggc gacttcacgg cgctgcgcgg cgaggtcggc gcgcgctgtc gtgcctgcta
     6061 cctgatcggc gaggccgccg accggttggc cgccgacctc gacgggaccg tgccgctgtg
     6121 tcgcagcggc accctggagc gagccgtcgc cgatgccagg gcggcgaccg agccgggcga
     6181 cgtcgtcctg ctctcacccg cctgcgcgag cttcgacgcc ttcgcgaact acgagcagcg
     6241 cggcgcccgc ttcaaggata tcgtcgccgg cctggagcgc gaagaatgag tttcgcgccg
     6301 tgcgtcgaag gggtgccgga tggcaaagcg cggcacgcgg ccgatcgagt actcgatcct
     6361 ctacaccgcg acgctctgca tgctcgccgt cggcgcggtg atggtgtact cggcgtcgtc
     6421 ggcggagtcg ctgctcgacg gctcgggcga cccttcgatg tacctcaagc gctacgtcgc
     6481 cttcggcctc gtcgggctcg gcgtgatgct gctcgccgct cgccacgggc tggcgctggt
     6541 gcgccagctg acgccctcgc tgctcggcgc ctcgttcttc ctgacgatcg cggtgatgct
     6601 gcccggcgtc ggcgtgacga tcaacggcgc cacccgctgg cttgccgccg gcccgctcca
     6661 gttccagccg tcggagctgc tgaaggtgtc gctggtgctc tacgccgcgc agctgctcgc
     6721 gacgcggccg cggtcggtcg agacgctcga gggcatcgcc aagccgctgc tgatcgtcgt
     6781 cggcgctgcg ttcctgctgt tgatgaagca gcccgacatg ggcacgacga tggtcatctg
     6841 cttcgcgatc ggctcgctgc tgatcgtcgc cggcacgccg atgcggctga tcgggatgct
     6901 ggccgggggg ctggcggtgc tggccttcgt cctggcgatc gtcgagccgt accggatgga
     6961 gcgcatgacg gccttcctca acccgttcgc ggacgccggc gacaccggct tccaggcggt
     7021 ccaggggctg acggcgattg gctccggcgg cctgttcgga gtcgggctcg gcgagtcggt
     7081 ccagaagatc ttctacctgc ccgaggcgca caccgacatg atcctggcga tcatcggcga
     7141 ggagctcggg ctggtcggca tcgcgggcgt cgcgggcctc tacgtgatga tcggctatgc
     7201 cgggctgcgt gccgcgaagg ccgcgcgcga ccgctactcg aagctggtgg cggcgggcat
     7261 cacgtcgctg atcctctgcc aggcgacgct caacttcttc gccgtgctcg gcctggcgcc
     7321 gctgaccggc gtgccgcttc cgttcatctc ctacgggagc accaacctga tcgtcctgct
     7381 cgctgggatg gggctgctgc tcaacgtcgc cgcgaccggc ggcgtcgcgg cgcgcggcgc
     7441 caagcccaag cgtgccggcg cggcggatgc cgacagatcg tccgcggtcc ggctgcgcgc
     7501 gatcgacgga ggtggcgccg atgaaggtcg cgatcgccgc cggcgggacg gcggggcacg
     7561 tcgtgccggc gctggcagtc gccgacgcac tgcgggctga aggcgccgcg gtcgagttcc
     7621 tcggcggcga gcgcgccgag gccgagctcg ttccggctgc cggctacccg ctccatccgc
     7681 tgcgcgtcgc cgggatcgac cgccgcaacc cgctgagggc cctgcgggcg ctcgccctcg
     7741 cagtacgcgc gaccgcgagg gcgcgccgcc tgctgcgcga gatcggagcc gacgtggtga
     7801 tcggcggcgg cggctacgtc gccggaccgg tcggcctggc cgcgcgcacg ctgaggctgc
     7861 cgctggtgct gacagaggcc gacagccacc tcgggatcgc caaccggctg ctcgcgccgg
     7921 cggcggcgcg cgtcttcctc gccttcccgc tcgccgggtg cgacggcgac cgctacgcgg
     7981 tggtcgggcg accgatcccg cgcggcacgc tcggcgccga ccgcattgcg gcacgcgccc
     8041 gcttcgggat cggcgccgac gagcgttgcc tgctcgtctt cggcggctcg cttggcgcgc
     8101 ggacgatcaa cgacgcgacc gtcgaggcgt tcggcgccgc cgcgccgtgc gcggtcctgc
     8161 acgcctgtgg tcgccgcgac cacgaggacc tcgccgaccg cctcgacaag ctcggctcgc
     8221 ccgcccacta ccgcctccac gactacatcc agcccttcgc ggacgcccta gccgccgccg
     8281 acctcgcggt cgcccgctcc ggcgggtcgg tcttcgaggt cgccgccgcg ggcgtcccct
     8341 cgatcctcgt gccgtatccg catgcgacag ccgaccacca gacgctcaac gcgcgccact
     8401 tcgccgccgg cggcgccgct gccgtcgtcc ccgacgacga gctcgacgcc gcacgcctgt
     8461 cgcgcgaggt cggcacgcta ctcgccgacg ccggccgcct ggacgagatg gggcgcgcgg
     8521 cacgcgcgct cgcgcggccc gatgccgccg agacgatcgc ccgcgaggtg ctcgaactcg
     8581 gtaaggcggc gacgaggtga gcgtggagcc gcccttcgcc gatcgccgcc tgcacttcgt
     8641 cggcatcggc ggcgccggca tgagcgggct cgccctggtc gccgcggagc tcggcgcgac
     8701 ggtcagcgga tccgaccgcg ccgcgtcctc ctacactgcc cgcctccgcg ccgccggcat
     8761 cgagcccgcg ctcggccacg acgccgccaa cctccccgcc ggcgccgagg ttgtcgtctc
     8821 gaccgctatc cccgccgaca accccgagct cgccgccgcc cgcgcggccg gcgccaaggt
     8881 catccaccgc ggccagctgc tcggcgagct gacccgcctc aagcgctcga tcgcgatcgc
     8941 cggcacccac ggcaagacca cgacctgcgg catggtcgtc cacgtcctgc gcgccaccgg
     9001 ccgcgacccg gcgttcgtca tcggcggcga gctgagctcc gccggcacca acgccgcctg
     9061 gggcccaggc gactggatcg tcgccgaggc cgacgagtcc gaccgctcct tcctcgagct
     9121 ccagcgcgac gtcgccgtcg tcaccaacat cgagctcgac caccacgcca cctacacctc
     9181 cctcacagac ctccaatcca ccttcgccca attcgcagcc cccgcaaacc tcacaatcgc
     9241 ctgggaccac atcgatcccg ccaccctcgc tccaaacacc cccaccgacg cccacgcgtc
     9301 catccctgac gcccaagtag ccgcgggaga cgaaggcc
//
LOCUS       DATABV010000158         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_612449, whole genome shotgun sequence.
ACCESSION   DATABV010000158 DATABV010000000
VERSION     DATABV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612449"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VHF88_07020"
     CDS             <1..205
                     /locus_tag="VHF88_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161556.1"
                     /translation="GDKIFDTPIRRSVAYAESAERAISIVDHRPDLGADYLSLADEVL
                     QRLGRDGDRKALKKLRAELVPAG"
     gene            215..1699
                     /locus_tag="VHF88_07025"
     CDS             215..1699
                     /locus_tag="VHF88_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX2161557.1"
                     /translation="MSDLLRKPASELASMVRSGDVSSRELVQASLDRIEATSELNHWT
                     LVDDENALAAADAVKPGDKRPFAGVPIAIKDLFTPVAGLRMAQGSDLLGEFTPDYDYG
                     QVRRLKEAGFVIVGKTQTPEFGITPVTNPRRFGPARNPWDPARTTGGSSGGSAGAVAS
                     GAVPIAHASDGGGSIRIPAACCGLLGLKPARGRISRAPDLGDHFLSTDGVLARSTEDV
                     AALLDLMAGPELGDATWAPPPEAPFAQLAQRDPGRLRIALVTDMPLPDAELDPECERG
                     AREAGELLEQLGHHVEHIPSPLPDGDAILGVFTALWAANVAASVMHGQIVSGNQPTAE
                     NIEPLSMWLYEMGQSIPSPAYIGAVVTSQAISRGVVSTWADWDLVVTPALAQRPVRHE
                     EIDPSGPDPEAQFKRAAEFTPYTPMFNLTGQPAISVPLFQGDDGLPTAAQIVGPQAGE
                     ALLLQVARQLEELAPWADRFPAGVPTPESLGAHPAEPGASARAS"
     gene            complement(1686..2078)
                     /locus_tag="VHF88_07030"
     CDS             complement(1686..2078)
                     /locus_tag="VHF88_07030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2161558.1"
                     /translation="MTVKRIEAAGGVVMRKGPEETEIAVVHRPRYDDWSFPKGKLDPG
                     ETFEEAALREVREETGLICRLGPELAFAHYDDNEGRPKVVRYWLMAVIEDPGFEPNDE
                     VDELRWLTPADATKLLTYSRDRKLVRTL"
     gene            complement(2075..2929)
                     /gene="thrB"
                     /locus_tag="VHF88_07035"
     CDS             complement(2075..2929)
                     /gene="thrB"
                     /locus_tag="VHF88_07035"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2161559.1"
                     /translation="MNRRRTVRVPASSANLGPGYDVLAAALSLSLELEVEETGTFSVD
                     CDTPGVPLDRDNLCVRAFERLHPADGVAFRIRSEIPSSGGLGSSAAAIVAGLAAADHL
                     YELDAPLLRHATELEGHPDNVAAALLGGFVICAEDAPVRLEPPAGLEGVLVTPPQPVP
                     TAEARAALPAEVPIDDAVHNVAHAALLALGLSSGDLGAIARGLSDRLHQDRRAHLYPR
                     SIELIGHATELGALGATISGAGPTALFWCQWEQTGAVVEGLRRLAPECDVRRVRFVAA
                     GADVEAIE"
     gene            complement(2926..>3214)
                     /locus_tag="VHF88_07040"
     CDS             complement(2926..>3214)
                     /locus_tag="VHF88_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2161560.1"
                     /translation="AMNAFTASRGQVRAVTDDQILDAYRLLASREGVFCEPASAASVA
                     GLLAHGGHGRVVCVLTGHGLKDPQTAMDRAASVVPCEPDIDSVETAVLGDG"
BASE COUNT          435 a         1216 c         1138 g          425 t
ORIGIN      
        1 cggcgacaag atcttcgaca ccccgatccg gcggtcggtc gcctacgccg agtcggccga
       61 gcgcgcgatc tcgatcgtcg accaccgccc cgacctcggg gccgactacc tgtctctggc
      121 ggacgaggtg ctgcagcggc tggggcgcga tggggaccgc aaggcgctga agaagctgcg
      181 cgccgagctg gtgccggccg gttaaggtcg gctcatgtcc gatctcctac gcaagccggc
      241 cagcgagctg gcctcgatgg tccgttccgg cgacgtctcg tcgcgcgagc tggttcaggc
      301 gtcgctcgac cggatcgagg cgacgagcga gctgaaccac tggacgctgg tcgacgacga
      361 gaacgcgctc gccgcggcgg acgcggtcaa gcccggcgac aagcgtccgt tcgccggcgt
      421 gccgatcgcg atcaaggacc tgttcacgcc ggtggccggc ctgcgcatgg cccagggttc
      481 ggacctgctc ggcgagttca cccccgacta cgactacggc caggtccggc gcctcaagga
      541 ggcgggcttc gtgatcgtcg gcaagaccca gacgccggag ttcgggatca cgccggtgac
      601 caacccgcgc cgcttcggcc cggcgcgtaa cccgtgggat cccgcgcgca cgaccggcgg
      661 ctcgagcggc gggtcggctg gagccgtcgc gtcgggcgcc gtcccgatcg cccatgccag
      721 cgacgggggc ggctcgatcc ggattcccgc cgcctgttgc ggcctgctgg gcctgaagcc
      781 ggcccgcggc cgcatctcgc gcgcgcccga tctcggcgac cacttcctct cgaccgacgg
      841 cgtgctcgct cgcagcaccg aggacgtcgc cgcgctgctt gacctgatgg ccggccccga
      901 gctcggcgac gcgacctggg cgccgccgcc cgaggcgccc ttcgcccagc tcgcccagcg
      961 cgacccgggc aggctgcgca tagcgctcgt caccgacatg ccgctccccg acgctgagct
     1021 cgaccccgag tgcgagcgcg gggcgcgcga ggcgggcgag ctgctcgagc agctcggcca
     1081 ccacgtcgag cacatcccct ccccgctgcc cgacggcgac gccatcctcg gcgtgttcac
     1141 cgccctctgg gccgccaacg tcgcggcgag cgtgatgcac ggtcagatcg tcagcggcaa
     1201 ccagccgacg gccgagaaca tcgagccgct ctcgatgtgg ctctacgaga tgggccagag
     1261 catcccctcg ccggcctaca tcggcgcggt cgtcaccagc caggcgatct cgcgcggcgt
     1321 cgtgtcgacg tgggccgact gggacctcgt cgtcacgccg gcgctcgccc agcgaccggt
     1381 tcgccacgag gagatcgatc ccagcggccc ggatcccgag gcgcagttca agcgggcggc
     1441 cgagttcact ccttacacgc cgatgttcaa cctgaccggc cagccggcaa tctcggtccc
     1501 gctcttccag ggcgacgacg gcctgccgac cgcagcccag attgtcggcc cgcaggccgg
     1561 cgaggcactg ctgctccagg tcgcgcgcca gctcgaggag cttgcaccgt gggccgaccg
     1621 cttcccggcg ggcgttccga cgcctgagag cctcggtgcg cacccggcag agccgggcgc
     1681 ttccgctaga gcgtcctgac cagcttgcgg tcgcgcgagt aggtcagcag cttggtcgcg
     1741 tcggcgggcg taagccagcg cagctcgtcg acctcgtcgt tgggctcgaa gccggggtcc
     1801 tcgatcaccg ccatcagcca gtagcggacg accttcggcc ggccttcgtt gtcgtcgtag
     1861 tgggcgaacg ccagctccgg cccgagccgg cagatcaggc cggtctcctc ccgcacctcg
     1921 cgcagcgccg cctcctcgaa cgtctcgccc gggtcgagct tgcccttcgg gaagctccag
     1981 tcgtcgtagc gcggccggtg cacgaccgcg atctccgtct cctccgggcc cttccgcatg
     2041 acgaccccgc cggccgcctc gatccgcttg accgtcactc gatcgcctcc acgtccgccc
     2101 ccgcggcgac aaatcgaacc ctccgcacgt cgcactccgg ggcaagcctg cgcagcccct
     2161 ccacgaccgc gccggtctgc tcccactggc accagaacag cgccgtcggc cccgcccccg
     2221 agatcgtcgc gccgagcgcg ccgagctcgg tggcgtggcc gatcagctcg atcgagcgcg
     2281 gatagaggtg cgcgcgccgg tcctggtgca ggcggtccga caggccgcgc gcgatcgcgc
     2341 cgaggtcgcc gctgctcagg ccgagcgcga gcagggccgc gtgggcgacg ttgtggaccg
     2401 cgtcgtcgat cggcacctcg gccggcagcg cggcgcgcgc ctcggcggtc ggcaccggct
     2461 gcggcggcgt gacgagcacg ccctccaacc cggccggcgg ctcgagccgc accggcgcgt
     2521 cctcggcgca gatcacgaag ccgcccagca gcgcggcggc gacgttgtcg ggatggccct
     2581 cgagctcggt cgcgtgccgg agcagcggcg cgtcgagctc gtagaggtgg tcggcggcgg
     2641 cgaggccggc gacgatcgct gcggcgctgg acccgaggcc gcccgaagac gggatctcgg
     2701 agcggatccg gaacgcgacg ccgtcggccg ggtgcagccg ctcgaaggcg cgcacgcaga
     2761 ggttgtcgcg gtcgagcggg acgccggggg tgtcgcaatc gaccgagaac gtcccggtct
     2821 cctcgacctc gagctcgagc gacagcgaca gcgccgcggc gagcacgtcg tagcccggcc
     2881 cgaggttggc cgaggacgcc ggcacgcgga cggtgcggcg gcggttcacc cgtcccccag
     2941 gaccgccgtc tcgacgctgt cgatgtccgg ctcgcacggg acgacagagg cggcgcggtc
     3001 catcgctgtc tgcgggtcct tcagcccatg gccggtcagg acgcatacga cgcggccgtg
     3061 gccgccgtgc gccagcagcc cggcgaccga ggcggccgac gccggctcgc agaagacgcc
     3121 ctcgcgcgag gcgagcagcc ggtaggcatc gaggatctgg tcgtcggtca ccgcgcgaac
     3181 ctgaccgcgc gatgcggtga acgcgttcat cgcg
//
LOCUS       DATABV010000159         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2379669, whole genome shotgun sequence.
ACCESSION   DATABV010000159 DATABV010000000
VERSION     DATABV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379669"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /locus_tag="VHF88_07045"
     CDS             complement(<1..2104)
                     /locus_tag="VHF88_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2161561.1"
                     /translation="MQQAMDRLTSFLERRRKPVLAFWGVLLVVSLPFAVKQTEHLTAG
                     GFTIPGSGSEAVDKAIADFDHAERQTLSVVVARRPGGDAADVRRELARVDAVVDDMPL
                     AELTQPALAAAERDAGSSPISIASLKTGGDMNDTADLAVDLREELGIGEGPRNGVETY
                     LVGEQALWAGMQDLSKKDLERAEATGFPIVLLILLAVFGSLVAALLPGVLGFVSVMVT
                     GAGIFFLSQATDMSVFVTNVASMIGIGVAVDYSLFILSRYREEINAGAEPIEARRIAM
                     RTSGVAVAFSGITVIVSLAGLLLVDSTTLRSMAYGAIMVVAVSIIAAMTFLPALMAAT
                     GRRGYVPGRTAGVVAGIVRGLRRLSRRQPRAEDAPGFWQSWTDRVTRRPGLTALAAAA
                     VMIVLAVPALSLETGDGALRQFPEGNETRVGAELAAKKAGHGAASPVEVVATFERGVA
                     GDPENREALKSFAAAVKDDPEAARVSPAVPSRDGSAALIAITPSTDAESQQARDFVER
                     LRDGESGAGALMEVARVDVGGATASVHDFNVQVTDSLWKIALFVLAFSYVVLFFLLRS
                     VLLPLKAVLMNLLSVGAAYGVLVAVFQWGWIDGFLGFQSLGYINTMTPPLLLAIVFGL
                     SMDYEVFLLSRIKERYAATGDSKVAVAQGLARSAATISSAALIMVAVFAVFAGVGVPS
                     VKEIGVGLAVAVSLDATIV"
     gene            complement(2217..>2725)
                     /locus_tag="VHF88_07050"
     CDS             complement(2217..>2725)
                     /locus_tag="VHF88_07050"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX2161562.1"
                     /translation="LSATVPQAGVSRGLLHYYFGSKERLLVEVVRQDCDRRVAALEER
                     LAAASTADETVAVLVESLQAFVADEPGAHAVLYEMLSASRHSEEIRVELVELYRTWRA
                     HMAAALRTKQQEGVIALDGDPEAVAALLFALGDGMGLQLISDPEWDSAATLELGMSTV
                     RRLLGSSS"
BASE COUNT          433 a          988 c          945 g          359 t
ORIGIN      
        1 gcacgatcgt cgcgtcgagc gagacggcga cggccaggcc gacgccgatc tccttgaccg
       61 acggcacgcc gacgcccgcg aagacagcga agaccgcgac catgatcagc gccgcgctgg
      121 agatcgtcgc cgcgctgcgc gctaggccct gcgcgaccgc gaccttcgag tccccggtgg
      181 ccgcgtagcg ctccttgatc cggctcagca ggaacacctc gtagtccatc gagaggccga
      241 agacgatcgc cagcagcagc ggcggcgtca tcgtgttgat gtagccgagc gactggaacc
      301 cgaggaagcc gtcgatccag ccccactgga agacggccac cagcacgccg taggcggcgc
      361 cgaccgagag caggttcatc agcaccgcct tcagcggcag caggaccgag cgcagcagga
      421 agaacagcac gacgtagctg aaggccagca cgaacagcgc gatcttccag agagagtcgg
      481 tcacctggac gttgaagtcg tggaccgacg ccgtggcccc gccgacgtcg acgcgggcga
      541 cctccatcag cgcgccggcg ccgctctctc cgtcccggag acgctccacg aagtcgcgtg
      601 cctgctggct ctcggcgtcg gtgctcggcg tgatcgcgat cagcgccgcg cttccgtcgc
      661 gcgagggcac cgccgggctg acccgggcgg cctccggatc gtccttcacg gccgccgcga
      721 agctcttcag cgcctcgcgg ttctcggggt cgccggccac accgcgctcg aaggtcgcga
      781 ccacctcgac cggcgaagcc gcaccgtgcc ccgccttctt ggcggcgagc tcggcgccga
      841 cgcgcgtctc gttcccctca gggaactggc gcagcgcgcc gtctccggtc tcgagcgaga
      901 gcgcaggcac cgccaggacg atcatcaccg ccgccgcggc cagcgcggtt aggccggggc
      961 gccgggtcac gcggtccgtc cagctctgcc agaagccggg cgcgtcctcg gcgcgcggct
     1021 ggcggcgcga gagcctgcgc aggccgcgga cgatccccgc cacgacgccg gctgtgcggc
     1081 cggggacgta gccccgccgt ccggtggccg ccatcagcgc cggcaggaag gtcatcgcgg
     1141 cgatgatcga gacggcgacg accatgatcg cgccgtaggc catcgaccgc agcgtcgtcg
     1201 agtcgacgag cagcaggccg gcgagcgaca cgatcacggt tatcccggag aaggcgacgg
     1261 ccactccgga ggtgcgcatc gcgattcgac gcgcctcgat cggctcggcg ccggcgttga
     1321 tctcctcgcg gtagcgggag aggatgaaca gcgagtagtc gaccgcgacg ccgatgccga
     1381 tcatcgaggc gacgttggtc acgaacaccg acatgtcggt ggcctgcgag aggaagaaga
     1441 tcccggcgcc ggtcaccatc acgctgacga agccgagcac acccggcagc aacgccgcca
     1501 ccagcgagcc gaagacggcg agcaggatca gcagcacgat cgggaagccg gtcgcctcgg
     1561 cgcgctcgag gtccttcttg ctgaggtcct gcatgcccgc ccagagcgcc tgctcgccca
     1621 cgagataggt ctcgacgccg ttccgcgggc cctcgccgat cccgagctcc tcgcgcaggt
     1681 cgaccgcgag gtcggcggtg tcgttcatgt cgcccccggt cttcagggag gcgatcgaga
     1741 tcggggagct gccggcatcg cgctcggccg ccgccagagc gggctgggtc agctccgcca
     1801 agggcatgtc gtcgacgacc gcgtcgacgc gcgcgagctc gcgccggacg tcggctgcgt
     1861 ctccgccggg ccgacgggcc acgacgaccg agagcgtctg gcgctcggcg tggtcgaagt
     1921 cggcgatcgc cttgtcgacc gcctcggaac ccgacccggg gatcgtgaag ccgcccgcag
     1981 tcaggtgctc ggtctgcttc accgcgaacg ggagcgatac gaccagcagc acgccccaga
     2041 acgccaagac cggcttgcga cggcgctcca ggaaggaagt cagcctgtcc atcgcttgct
     2101 gcatcgagcg ctccttcggc gggggtttcg gctggctggt tggctagcca gccagcaaga
     2161 tacgccaatc cgatccgatc ggcatcggcc ccactgtgat tctcgtcaca gagcggctag
     2221 gagctgctgc cgagcaggcg cctgacggtg ctcatgccca gctcgagggt cgcggcgctg
     2281 tcccactccg ggtccgagat cagctgcagc cccataccgt cgccgagcgc gaacagcagc
     2341 gccgcgacgg cctcgggatc gccgtccagc gcgatcacgc cctcctgctg cttggtgcgc
     2401 agcgcggccg ccatgtgcgc gcgccaggtg cggtagagct cgacgagctc gacgcggatc
     2461 tcctcgctgt ggcgcgaggc gctcagcatc tcgtacagca ccgcgtgggc gcctggctcg
     2521 tcggcgacga acgcctgcag cgactctacg agcaccgcga ccgtctcgtc ggcggtcgac
     2581 gcggccgcga gccgctcctc gagcgcggct acgcgccggt cgcagtcctg gcggacgacc
     2641 tccaccagca gccgctcctt ggagccgaag tagtagtgca ggagcccgcg cgagacgccg
     2701 gcctgtggga cggtcgcact gaggc
//
LOCUS       DATABV010000160         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2138951, whole genome shotgun sequence.
ACCESSION   DATABV010000160 DATABV010000000
VERSION     DATABV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138951"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VHF88_07055"
     CDS             complement(<1..346)
                     /locus_tag="VHF88_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2161563.1"
                     /translation="MSRVYLDTNVFIYAIGGDSPHREPSREILRQVAERRLAGETSAY
                     ALQEMARQRRRRGDTEMTKRIREAAALCSTIHPLDWQVLANALRHLGVPPAPALTGHL
                     LQRIRARLACQAP"
     gene            complement(343..606)
                     /locus_tag="VHF88_07060"
     CDS             complement(343..606)
                     /locus_tag="VHF88_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX2161564.1"
                     /translation="MGELNRRVHVLLDDDRLERLRQRAAATGSSVGAVVREAIDNELA
                     RDDRAPALAALKSFLAAPPLPVGRPDELKRELQEAWDEEIGID"
     gene            complement(655..>2646)
                     /locus_tag="VHF88_07065"
     CDS             complement(655..>2646)
                     /locus_tag="VHF88_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161565.1"
                     /translation="TAMPASAAAADVYAYANGCYSLRDATTDRVVVRDALGYALKAPG
                     AATPTPFRLQATALGRYLLFGADGRMPAASVLGAVSANANPGPAADWRATEVDDVLRL
                     TNVATDKHLGVGAFGRLAQVASTTPRWSFEPAQGCAEFPEVEVNVSGEPFKGVSPDAP
                     VRGFLDDHIHLGAYRFLGGRFHCGRPWSPYGVTVAMRDCVDHVGNGVGAVAENFFAFG
                     SPVGTHSTDGWPSFAGWPRDESLTHEGTYWKWIERAWRSGLRIMVNDLVENRALCEIY
                     PLKQNNCNEMVSAYRQAEDMHALVDYIDAQFGGPGKGFLRIVDDPAEARRVVNEGKLA
                     MVLGIEVSEVLDCGQFNGVPRCTTAQIDAELDRLEAIGVQSLFPVHKFDNALGGVHFD
                     SGTQGVLVNTGNKYATGQFWTAEHCPGAADHDNEPTNPTGEHAALIYNVFGSVLTAPL
                     LEGQLPVYPPAPLCNPKGLTPLGEHLIRSMIDRGMIVETDHLSVKARGEALTILEQEG
                     YPGVVSSHSWGDDGSQQRLQALGGLVGPISHEANHFVEEWQAARANRRQDQLFGLGFG
                     SDINGLHAQPVPRPDAAQNPVQYPFQSFDGGTTIHRQVSGTRTYDVNVDGVDHYGLYP
                     DWIEDLRIVGGPQIIEDLANGAEAYLRLWEQAEAAAAAP"
BASE COUNT          385 a          921 c          917 g          423 t
ORIGIN      
        1 gcggcgcctg gcaggcgaga cgagcgcgta tgcgctgcag gagatggccc gtcagcgccg
       61 gcgcaggggg gacaccgaga tgacgaagcg cattcgcgag gacctgccag tcgagtgggt
      121 ggatcgtcga gcagagcgca gctgcctcgc gaatgcgctt cgtcatctcg gtgtcccccc
      181 tgcgccggcg ctgacgggcc atctcctgca gcgcatacgc gctcgtctcg cctgccaggc
      241 gccgctccgc cacctggcgc aggatctcgc gcgaaggctc ccgatgaggg gagtccccgc
      301 ctatcgcata gatgaacacg ttcgtgtcga ggtagacgcg gctcaatcga ttccgatctc
      361 ctcgtcccat gcctcctgca gctctcgctt gagctcatcg ggtctgccga cgggcagcgg
      421 cggggcggcg agaaaggact tcagcgcggc gagcgcgggg gccctgtcat ctcgtgcgag
      481 ctcgttgtcg atcgcctcgc ggaccaccgc tccgaccgac gaaccggtgg ccgccgcacg
      541 ctggcggaga cgctcgaggc ggtcgtcgtc gagcagaacg tgcacacgcc tattcaactc
      601 acccatatgt gcaggttaca tgcacatatg tgacgatcta gcttcgcggg cgcgttacgg
      661 ggctgccgcc gcggcctccg cctgctccca gagccggagg taggcctcgg cgccgttggc
      721 gaggtcctcg atgatctggg ggccgccgac gatgcgcagg tcctcgatcc agtcgggata
      781 gaggccgtag tggtcgacgc cgtcgacgtt gacgtcatag gtgcgggtgc cggagacctg
      841 gcggtggatc gtggtgccgc cgtcgaagga ctggaacggg tactggaccg ggttctgcgc
      901 cgcgtccggg cgggggaccg gctgggcgtg caggccgttg atgtcggagc cgaagccgag
      961 cccgaagagt tggtcctggc ggcggttggc gcgcgccgcc tgccactcct cgacgaagtg
     1021 gttcgcctcg tggctgatcg ggccgacgag gccgccgagc gcctggaggc gctgctggct
     1081 gccgtcgtcg ccccagctgt ggctcgagac cacgccggga taaccctcct gctcgaggat
     1141 cgtcagcgcc tcgccgcgcg ccttgacgct gaggtggtcg gtctcgacga tcatcccgcg
     1201 gtcgatcatc gaccgaatca ggtgctcgcc aagcggcgtc aagccctttg ggttgcacag
     1261 cggcgccggc ggatagacgg gcagctggcc ctccagcagc ggcgcggtca gcaccgagcc
     1321 gaagacgttg tagatcagcg cggcgtgctc gccagtcggg tttgtcggct cgttgtcgtg
     1381 gtcagcggcg ccggggcagt gctccgcggt ccagaactgg ccggtcgcgt acttgttgcc
     1441 ggtgttgacg agcacgcctt gagtcccgga gtcgaagtgg acaccgccga gcgcgttgtc
     1501 gaacttgtgc acagggaaca gggactggac gccgatcgcc tccaaccggt cgagctcggc
     1561 gtcgatctgc gccgtcgtgc agcgcgggac gccgttgaac tggccgcagt ccaggacctc
     1621 cgagacctcg atcccgagga ccatcgccag cttgccctcg ttgaccacgc gccgcgcctc
     1681 ggcggggtcg tcgacgattc gcaggaaccc ctttcccggc ccgccgaact gggcgtcgat
     1741 gtagtcgacc agcgcgtgca tgtcctcggc ctgccgatac gcgctcacca tctcgttgca
     1801 gttgttctgc ttgagcgggt agatctcgca cagcgcccgg ttctcgacga ggtcgttgac
     1861 catgatccgc agccccgacc gccaggcgcg ctcgatccac ttccagtagg tcccctcgtg
     1921 ggtcagcgac tcgtcgcgcg gccagcccgc gaagctcggc cagccgtcgg tgctgtgcgt
     1981 gccgaccgga ctgccaaagg cgaagaagtt ctccgcgacc gccccaacgc cgttgccgac
     2041 gtggtcgacg cagtcgcgca tcgcgaccgt gacgccatac gggctccacg gccgcccgca
     2101 gtggaaccgc ccacccagga agcggtacgc cccgaggtgg atgtggtcgt cgaggaagcc
     2161 gcgcaccggc gcgtcggggc tcacgccctt gaacggctcg ccgctgacgt tgacctcgac
     2221 ctccgggaac tccgcgcatc cctgcgccgg ctcgaagctc cagcgcggag tcgtggaggc
     2281 gacctgcgcc agccggccga acgctccgac gcccaagtgc ttgtcggtcg ccacgttcgt
     2341 caaccgcagc acgtcgtcga cctcggtcgc gcgccagtcc gccgccggcc cgggatttgc
     2401 gttcgccgag accgccccga gcaccgacgc cgccggcatc cgcccgtcgg ccccaaagag
     2461 cagatagcgc cccagcgccg tggcctgcag ccggaacggc gtcggcgtcg ccgcaccggg
     2521 ggccttcagc gcatagccga gcgcgtcgcg gacgacgact cgatccgtcg tcgcgtcgcg
     2581 cagcgagtag cagccgttcg cgtacgcgta tacgtcagct gctgcggccg acgccggcat
     2641 tgcggt
//
LOCUS       DATABV010000161        10353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2301044, whole genome shotgun sequence.
ACCESSION   DATABV010000161 DATABV010000000
VERSION     DATABV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301044"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1264)
                     /locus_tag="VHF88_07070"
     CDS             complement(71..1264)
                     /locus_tag="VHF88_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX2161566.1"
                     /translation="MTKPRPQLTAVLLAAIVALAAAALAPTAASGAVQVVRGAGFGHG
                     IGMSQYGAYGMAQEGWGYERILRHYYRGTKLGQAPSRPVRVLLQASDPYVRFSGATRG
                     PGGIALSRGATHVVRPARGNRLALYGAGGRRLGTYRAPLTVSNGGKPMRLLGAAIQGV
                     VSGRYRGSFDLYPGSAGGVTVVNALPIDDYVKGVVASEVPASWAADVLRAQAVTARTY
                     ALTTRKTGDIFDQYPDTRSQMYKGLVAETAATNAAVRRTAGEIVTHDGEPATTFYFST
                     SGGRTENIENSWPGSSPLPWLKSIHDPYDAISPKHRWTVRFSNARMGALLGAPGTLRR
                     VEVLDRGVSPRIVRARIVGSRGSTILTGPQIRARLGLDDTWAYFTRVGSARAAAKLAR
                     RWVGR"
     gene            complement(1671..2285)
                     /locus_tag="VHF88_07075"
     CDS             complement(1671..2285)
                     /locus_tag="VHF88_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161567.1"
                     /translation="MRKLILACLAAVAIMGAVAAPASADVDVHDANTDTKCSQLWLGS
                     GQVTGGCPLRLEGPLNFDLGPVFQGLFPYPVFGCTISASGRVGGEGHFVFAEAAVSGP
                     RPCADAQYSACSVVDHPAGKWGGTFTEEGPNLSDPFKATLNMCLEHFGDVTIEFDVLH
                     NYAGGDNFPLQLFSSDVMGISYYSQVRVRGMLGSPDSSGIRIYH"
     gene            complement(2394..5684)
                     /locus_tag="VHF88_07080"
     CDS             complement(2394..5684)
                     /locus_tag="VHF88_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161568.1"
                     /translation="MSLVLLLLSPWSASADHGEPLGANFGIADAAGPAWPGAKAWWAG
                     TCDLSAASTTGGGVGPHNGGTAPPPNFAHCIDHGGPYSPTEVDATPPLDTTWTPGAEP
                     SWRLDPVAQAGAHPDATASFWMQRSPDPSLSGVLPILSIGDGDTRTVEVKLPPGLVGN
                     PNAVPACSSADARRSPLGCGPETQVGVSTITTSSSGGTATSVFAVYNVEPRDGKTAEF
                     LINAAPNAIVRANIPIVAKARTSGDFGIDTMVLDVPGGLPLLGQSITLWGVPWASSHD
                     RYRPRTGYLGHTTDGEVLGGMPEAGLTGDTPDAEPASYDPSWGPIRPFFTNPTECDPV
                     APVTIVEVSSWQDPGVFKPYKAPADVPVDGCAAVPFQPSFDMRATSTVANDATGLEAD
                     MTIPQNDDPPASVAHDPDDATGAPAHWKSPAGLATAQLDKAVVTLPPDVAINPAGAAG
                     LEGCSDEQIGLVAVGNPPTFNDEDPFDGAGAECPAGSRIGTAEVFTPILPGADEQSRT
                     PNLTGEVVLGTPKSINPESGDMFRLFLVVRNRQRGLLAKIAGSTVVDAQTGRITATFD
                     KNPRVPFETMKLRLKGGAHGLLALPPRCESAGWSSLFTPWTAAHGAGGRAVGAGGSFV
                     TNTRCSLGFSPTLRAGMSSQQAVGGGSFSFQLTRGDGDQFLSELSATMSSGLLASVRS
                     VPLCSNGQAAANACPSSSQIGVVDAGAGSGAPYFLERKGSVFLTEGYKGAPYGLAVSV
                     PVEAGPFKGPLALSTIVVRQALHVDRRTAQVTVVSDPFPRIWHGIPLRVRQVTVAVNR
                     PGFMVNPSDCSPKQIAATVLSTEGARADLAYPFQASGCAALRFRPKIRIALTGKRQRR
                     TGKHPGVRAVVTQRAGEARIEKAVVRLPKSLALDPDNARALCEFEDGTRDDLENHCPR
                     GSIVGRARAVSPLLKRPLVGNVYFVKNVRIDKRTGNEIRTLPMIVVALRGEIAINLRG
                     ESSTTKGGKLVNTFAGVPDAPVSRFNLNIKGGKNGILTVTRTRRARIDLCAKPKGHRA
                     VTDVDGHNGKHFDRNIRVKTPCGKRSKAQRRKAAAKRRKAAARRRARQGPDRQDRPSA
                     RRSSTPSG"
     gene            complement(5807..10210)
                     /locus_tag="VHF88_07085"
     CDS             complement(5807..10210)
                     /locus_tag="VHF88_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161569.1"
                     /translation="MSWLVIVVAALGLAVMAAPAHAGKTVDSFFGGPGTSGGEFQGVG
                     DVALNQVTGDAYVSDYAVERIQRFSAADDFELTWGQNVDVSGGTGFEICTVAANCQRG
                     ESGAAAGQLFLPSGLAISEDDGSVYVAEGGNRRVSKFDADGNFLFAFGWGVDTGAAAL
                     EICTIATTCQRGVSGSGAGQLTGANRTDVEVDQATGDVLVTDPGNRRIQRFDSSGGFL
                     RAFGWGVDTGAAALETCTAASGCQEGISGSGVGQFGSTSPVALAVDASGDIYALDHAN
                     GRILRFDPSGATATVFGADVIPQSPTVLAVDPSTDHVLVGQHDSAAGEARIKELDSAG
                     ALVDTHLADAGLDAVSMRGLAVSGSTGDIYVTNGTHNVVLILDDDGAPQPALTIAPAT
                     GVTAHGATLHGTVDSNTGAASYRFEYSRDGVTWIDAGSGGVVTGDDPVTVTETVSTLE
                     ANSIYRVRLVATKRFGGPTAVSAETTVTTPVSPPDVTTGASQHVAATSARLLGRVNPN
                     NLPTTYYFELGTSADYGSRIPIPAADAGSAGIVRAVAQDASGLTPATTYHYRLVATNS
                     IGTSEGEDRTFTTRGAVAPPPGRAYEMVTTPDKNNRRGGESMPDHAAANPGTPSADGE
                     SLLFGLRFGILDREAGTAFPHQDDMVVIRRTAAGWRSDAVQNIAGAESAALPVNFATG
                     VSADLDTQAWQHRAWQFPSGSRLGTRVFGDGGGLRGSGWYDWIVDPLLAEQAVNTDSA
                     LIDDDGDRMLRWGGPQETYRGLLGPTPADDPSNLQIAGDAIYLQEPPGSGPRHLVNEC
                     TGTTAGGDATMIPTRQANGTIAARPCERGAVTSVRGASVGAGGEVGRANPIAGPSATA
                     MSDDGRRVFFMSPDPLDDATPTFCATGTGAATDCPPQLYVRQYGPDGATVRWISRSEI
                     AGQSFGLLGRGTSFEGASRDGRFVYFRTNAPLTEDDPNGTGTAGPILAGGAADDSWDL
                     YRYRLPDDLADDPADGDLTRISGGPSGTADPSTHCMIGAGCGVGARSGTAARYISDDG
                     RRAYFVTASPIDGADATPPANGTTTPGGTPTGNASVRNLYLYDDRLSGGDRWRFVARL
                     PYSASGSAVDACATANGVTGMPQALNSDPRGLLLRAQVNCVRGTPDGDAVVFETAGQL
                     TEDDDDSASDVYLYEADTDRLTRVSAPLPGASPYRCADGFDATLEQLCNADLGFQGPM
                     YGEGERVGLGGQRHANLALHGERGLSIYFESRLPLVEGDTNGDRMDVYEWRDGKLTLI
                     SPGNTHDDAWYSGNSRDGQDVFFQTSQRIDLREIEDADFDIYDARVGGGFPPPSPPPV
                     PCDPLAGACQGPGGPPMGISERTHRSGGSNADTARVGLRLARVGRKARRRAARTGVVR
                     VRLRITAPGKVTAVARARIRTTKRRSRVRTVARRSIRVRRAGSAVIRLRLNRPARRQL
                     AAGRALRLTVTVRSPRAGERSARVRLRRAGR"
BASE COUNT         1462 a         3598 c         3710 g         1583 t
ORIGIN      
        1 gccgcgcctt cgcgcggctc cagtgcttct gggacgggcg gttggccagg gcgcgcaggc
       61 ccgaacggca ctagcggccg acccagcgtc gcgccagctt ggcggcggcg cgcgccgacc
      121 cgacgcgcgt gaagtaggcc caggtgtcgt cgaggccgag gcgggcgcgg atctgcgggc
      181 cggtgaggat cgtcgagccc ctcgagccga cgatccgcgc gcgcacgatg cgtggggaga
      241 cgccgcggtc gagcacttcg acgcggcgca gcgtgcccgg cgcgccgagc agcgccccca
      301 tccgcgcgtt cgagaagcgg acggtccagc ggtgcttcgg cgagatcgcg tcgtaggggt
      361 cgtggatgct cttcagccac ggcagcggcg aggaaccggg ccagctgttc tcgatgttct
      421 cggtgcgccc gcccgaggtc gagaagtaga aggtggtggc cggctcgccg tcgtgggtga
      481 cgatctcgcc ggcggtgcgg cggacggcgg cgttggtggc ggccgtctcg gcgaccaggc
      541 ccttgtacat ctgggagcgg gtgtcggggt actggtcgaa gatgtcgccg gtcttgcgcg
      601 tcgtgagcgc ataggtgcgc gcggtgaccg cctgggcgcg caggacgtcg gccgcccatg
      661 aggccggcac ctcgctggcc acgacgccct tcacgtagtc gtcgatcggc agcgcgttga
      721 cgaccgtcac gccgccggcg ctgccggggt agaggtcgaa gctgccacgg tagcggccgc
      781 tgacgacgcc ctggatggcc gcgccgagca ggcgcatcgg cttgccgccg ttgctgacgg
      841 tcagcggcgc gcggtaggtg ccgaggcggc ggccgccggc gccgtagagc gcgaggcggt
      901 tgccgcgcgc ggggcggacg acgtgggtgg cgccgcgtga cagcgcgatg ccgccgggac
      961 cgcgcgtcgc gccgctgaag cgcacgtacg ggtcgctggc ctggagcagc acgcggaccg
     1021 gccgactcgg cgcctggccg agcttcgtcc cccggtagta gtggcgcagg atccgctcgt
     1081 agccccaacc ctcctgggcc atcccatagg cgccgtactg gctcatgccg atgccgtggc
     1141 cgaagccggc tccgcggacc acctggacgg cgccgctggc ggcggtggga gcgagcgcgg
     1201 cggccgcgag cgcgacgatc gctgcgagca ggacggcggt cagctggggg cgaggcttgg
     1261 tcatagtcgg ttccaaccag ttcggcggcc caaggtagcg gtccttccgc ccgccgcgcg
     1321 cgaccgcggc ggcgcatccg caatcgttct gcccttgact ttcgccgccc attgcggata
     1381 gggtcgtgtc tccaacccag atcccggttg gagccctcct tttggagggg aggagggaag
     1441 cgttaggact cgagtcccgg ccgatacccc ggggcgccag acctccacac aggaggggtg
     1501 cggagcgtcg tctcagagtc cagatacgcg gggcaggtgg cggggcccac accgccacca
     1561 ggccctgcgc agctcgaaac cacggtcgtc gtacggcgca aagggcgccg gccgatacgg
     1621 ccgacgccct cgcccgatcg gtgcgagtag agctcacacc ttcgatgcgg ctagtgatag
     1681 atccggatgc cagacgagtc cggtgatccc agcatgccgc ggacgcgcac ctgcgaatag
     1741 tacgaaatcc ccatcacatc cgacgagaac agctgcagcg gaaagttgtc acctcccgca
     1801 tagttgtgca aaacgtcaaa ctcgatggtg acatcgccga aatgctccag gcacatatta
     1861 agggttgcct tgaatggatc actgaggttg gggccctcct cagtgaaagt tcccccccac
     1921 ttcccggccg gatgatcgac aacagaacat gctgagtatt gagcatcagc acaagggcgt
     1981 ggacccgaaa cagctgcctc cgcgaagaca aagtgtcctt ctccaccgac acggccagat
     2041 gcggagatcg tgcacccgaa gacgggatag ggaaacaacc cctggaacac cggccccaga
     2101 tcgaagttca gcggcccttc cagccggagc gggcagccgc cagtgacttg cccggaaccg
     2161 agccagagct ggctgcactt ggtgtctgtg ttcgcgtcgt ggacgtcgac atccgccgat
     2221 gccggcgctg ccacggcgcc catgatcgcc accgcggcga gacaggcgag aattagcttt
     2281 cgcatactac tcctttgatt tcgtgtttaa ccgggctgac gcccggcact gcaggccgcc
     2341 gatcgccggt cacgcggcga tcggctgtgc cgttttgtga agggctgggg tcgctagccg
     2401 gagggcgtgg agctgcgcct cgcggaggga cggtcctgac gatctggccc ctgtcgcgcc
     2461 cggcgcctcg ccgcagcctt gcggcgcttc gccgccgcct tgcgacgttg cgccttcgac
     2521 cgcttaccgc acggtgtctt cacgcggatg ttgcggtcga agtgcttgcc gttgtggccg
     2581 tcgacgtcgg tcacggcccg gtgccccttg ggcttggcgc agaggtcgat cctggcgcgc
     2641 cgggtgcggg tcaccgtgag gatgccgttc ttgccgccct tgatgttgag gttgaagcgg
     2701 gagaccggtg cgtcgggcac gcccgcgaac gtgttgacga gcttgccgcc cttggtagtc
     2761 gagctctcgc cgcggaggtt gatcgctatc tcgcctctca gcgcgacgac gatcatcggc
     2821 agcgtgcgga tctcgttgcc ggtccgcttg tcgatcctga cgttcttgac gaagtagacg
     2881 ttgccgacca gcgggcgctt gagcagcggt gagaccgccc tcgcccggcc gacgatcgac
     2941 ccgcgcgggc agtggttctc gaggtcgtcc cgcgtcccgt cctcgaactc gcacagcgct
     3001 cgcgcgttgt cggggtcgag cgcgagcgac ttcggcaggc ggaccacggc cttctcgatg
     3061 cgcgcctcac ccgcgcgctg ggtcacgacc gcgcgcacgc cgggatgctt gcccgtccgc
     3121 cgctggcgct tgcccgtgag cgcgatccgg atcttcggac ggaagcgcag cgctgcgcag
     3181 ccgctcgcct ggaagggata ggcgaggtcc gccctcgcac cctcggtcga cagcaccgtc
     3241 gcggcgatct gcttcggcga gcagtcgctc gggttcacca tgaagccggg cctgttgacg
     3301 gcgaccgtca cctgacggac gcgcagcggg atcccatgcc agatccgcgg gaacgggtcc
     3361 gacacgacgg tcacctgcgc cgtgcggcga tcgacgtgca gcgcctggcg gacgacgatc
     3421 gtcgacaggg cgagcggccc cttgaacggc cccgcctcga cgggcaccga gacagccaag
     3481 ccgtacggcg cgcccttgta gccctcggtg aggaagaccg agcccttccg ctcgaggaag
     3541 tacggcgccc cggaccccgc gccggcatcg acgacgccga tctgcgagga cgacggacag
     3601 gcgttcgccg cggcctgccc gttgctgcag agcggcaccg accggaccga ggcgagcagg
     3661 cccgacgaca tcgtggccga cagctcgctg aggaactggt cgccgtcgcc gcgcgtcagc
     3721 tggaacgaga agctgccgcc gccgaccgcc tgctgcgagc tcatgccggc tctcagcgtc
     3781 ggcgagaagc cgagcgagca gcgcgtgttg gtcacgaagc tgccgcccgc tcccaccgcg
     3841 cggccgcccg cgccgtgagc ggcggtccat ggagtgaaca gcgacgacca gccggcgctc
     3901 tcgcagcgcg gcggcaacgc gagcagtcca tgcgcgcccc ccttgagccg gagcttcatc
     3961 gtctcgaacg ggacccgcgg gttcttgtcg aaggtcgccg tgatgcgccc ggtctgggca
     4021 tccaccaccg tcgatcctgc gatcttcgcc agcaggccgc gctgcctgtt gcgcacgacc
     4081 aggaacagcc ggaacatgtc gcccgactcg gggttgatcg acttcggcgt cccgagcacc
     4141 acctcgccgg tcaggttcgg cgtgcgcgac tgctcgtcgg cgcccgggag gatcggcgtg
     4201 aagacctccg cggtcccgat cctcgagccg gccgggcact cggcgcccgc gccgtcgaac
     4261 gggtcctcgt cgttgaaggt cggcgggttg ccgacggcga cgagcccgat ctgctcgtcc
     4321 gagcagccct cgaggccggc ggcgccggcg gggttgatcg cgacgtccgg cggcagcgtg
     4381 acgaccgcct tgtcgagctg cgccgtcgca aggccggcgg gcgacttcca gtgcgccgga
     4441 gcgccggtcg cgtcgtccgg gtcgtgggcg accgacgcag gcggatcgtc gttctgcggg
     4501 atcgtcatgt cggcctcgag cccggtcgcg tcgttcgcga ccgtcgaggt ggcgcgcatg
     4561 tcgaagctcg gctggaacgg caccgcggcg cagccgtcga ccgggacgtc ggcgggagcc
     4621 ttatacggct tgaagacgcc cggatcctgc cacgagctga cctcgacgat cgtcacgggc
     4681 gcgaccgggt cgcactcggt cgggttcgtg aagaacggcc tgatcggacc ccacgacggg
     4741 tcgtaggacg ctggctcggc gtcgggcgtg tcgccggtca ggcctgcctc cggcatgccg
     4801 ccgaggactt cgccgtcggt agtgtggccg aggtagccgg tgcgcgggcg gtacctgtcg
     4861 tgcgaagacg cccacggcac gccccacagc gtgatcgact ggccgagcag cgggaggccg
     4921 cccggtacat cgaggaccat cgtgtcgatt ccgaagtcgc cgctggtgcg agccttggcg
     4981 acgatcggga tgttcgctcg cactatcgcg tttggcgcgg cgttgatcag gaactcggcc
     5041 gtcttgccgt cgcgcggctc gacgttgtac acggcgaaca cgctcgtcgc cgttcccccg
     5101 gagctgctgg tggtgatggt cgagacaccg acctgggtct cgggcccgca tcccagcggg
     5161 cttcggcgcg cgtccgcaga cgaacaggcc ggtaccgcgt tggggttgcc taccagtccc
     5221 ggcggcagct tcacctcgac ggtcctggtg tcgccatcgc cgatcgagag gatcggcaat
     5281 accccggaga ggctcgggtc gggggatcgc tgcatccaga acgacgcggt cgcatcgggg
     5341 tgcgcgcccg cctgggcgac cgggtcgagc cgccagctcg gctccgcgcc aggagtccag
     5401 gtcgtgtcga gcggcggggt cgcgtccacc tcggtggggc tgtacggccc gccgtggtcg
     5461 atgcagtggg cgaagttggg gggaggggcg gtgccgccgt tgtgcgggcc aactccgccc
     5521 ccggtcgtgc tcgccgcgct gaggtcgcag gtgccggccc accacgcctt cgcaccgggc
     5581 caggccggtc cggccgcgtc ggcgatgccg aagttggcgc ccagcggctc gccgtggtcc
     5641 gcactcgccg accaagggga cagcagcagg aggaccaggc tcatcgacgc gacgagagcc
     5701 gtcgcgcgac gcgctcgccg ccagagggat gtgtgctcat gccgggcctt cgcgccaatc
     5761 atcgtggggg tgcctctcgt cgtcgccggg tcgtttgctg atgccctcac cggccggccc
     5821 tccggagccg gactcgcgca gaccgctcgc cggccctcgg gctcctaact gtcaccgtca
     5881 gcctcagcgc gcgaccggcc gcgagctggc gcctggcggg ccggttcagc cgtaggcgga
     5941 tcaccgcgga gccggctcgg cggacgcgga tgctccggcg agcgaccgtg cggacgcggc
     6001 tcctccgctt ggtcgtccga atacgcgcgc gcgccaccgc ggtcaccttg cccggcgccg
     6061 tgatgcgcag gcggacgcga acgactcccg tgcgcgccgc gcgccgacgg gcctttcggc
     6121 cgacccgcgc aagcctcagg ccgaccctgg ccgtgtcggc attcgacccg cccgaccgat
     6181 gggtccgctc cgagataccc atcggcggcc cacccggccc ctggcaggcg cccgcgagcg
     6241 ggtcgcaggg gaccgggggt ggggacggtg gcgggaagcc gccgccgacg cgggcgtcgt
     6301 agatgtcgaa gtcggcgtcc tcgatctcgc gcaggtcgat ccgctgagag gtctggaaga
     6361 agacgtcctg gccgtctcgg ctgttgccgg agtaccacgc gtcgtcgtgc gtgttgcccg
     6421 gcgagatcag cgtcaacttg ccgtcgcgcc actcgtagac gtccatccgg tcaccgttgg
     6481 tgtctccctc gacgagcggc aggcggctct cgaagtagat cgagaggccg cgctcgccgt
     6541 ggagtgcgag attggcgtga cgctggccgc cgaggccgac tcgctccccc tcgccgtaca
     6601 tcggcccctg gaacccgagg tcggcgttgc acagctgctc cagcgttgcg tcaaagccat
     6661 ctgcgcaccg atacggcgag gcgcccggca gcggcgccga gacgcgggtc aggcggtcgg
     6721 tatccgcctc gtagaggtag acgtcgctcg cgctgtcgtc gtcgtcctcg gtcagctggc
     6781 cggccgtctc gaagaccacg gcgtcgccat cgggcgtgcc gcgcacgcag ttgacctgcg
     6841 cgcgtaagag aaggccgcgg ggatcggaat tcaatgcctg tggcattccg gttactccgt
     6901 tcgcggtcgc acaagcgtcc acggcgctcc ccgaggccga gtacggcaga cgcgccacga
     6961 accgccagcg gtcgccgccg ctcagcctgt cgtcgtacag gtagaggttg cggacggagg
     7021 cgttgcccgt cggcgtcccg ccgggagtcg tcgtcccgtt cgcggggggc gtcgcgtcgg
     7081 cgccgtcgat cggagacgcc gtcacgaaat aggcgcgcct gccgtcgtcg gagatgtagc
     7141 gcgcggccgt gccggatcgc gccccgaccc cgcagccggc accgatcatg cagtgagtgc
     7201 tcggatccgc ggtcccgctc gggccgccgg agattcgggt gaggtcgccg tcagcggggt
     7261 cgtccgcgag atcgtcggga agccgatagc ggtacaggtc ccaggagtcg tccgcggccc
     7321 ctccagcgag gatcggaccc gctgttccgg tcccgttggg gtcgtcctcg gtcaaggggg
     7381 cattggtcct gaagtagacg aacctcccgt cgcgcgacgc accctcgaag gaggtgccgc
     7441 gtccgagcag cccgaagctc tgcccggcga tctccgagcg ggatatccat cggaccgtcg
     7501 cgccgtccgg gccgtactga cgcacgtaca gctgcggcgg gcagtcggtg gccgcgccgg
     7561 tgccggtggc gcagaaggtc ggggttgcgt cgtcgagcgg atccgggctc atgaagaaga
     7621 cccgccggcc gtcgtccgac atcgccgtgg ccgacggtcc tgcgatcggg ttcgcgcggc
     7681 ccacctcgcc ccccgctccg acggaagctc cgcggacgct cgtgacggcc ccgcgctcgc
     7741 agggccgggc ggcgatcgtc ccgttcgcct ggcgagtcgg gatcatcgtc gcgtcgccgc
     7801 cggccgtggt gcccgtgcac tcgttgacga ggtggcgcgg gccggatccc ggtggctcct
     7861 gcaggtagat cgcatccccg gcgatctgga ggttgctcgg atcgtcggcg ggcgtcggtc
     7921 cgagcaggcc gcggtaggtc tcctgcgggc cgccccaccg cagcatccgg tcgccgtcgt
     7981 cgtcgatcag cgcgctgtcc gtgttcaccg cctgctcggc gagcagcgga tcgacgatcc
     8041 agtcgtacca tccggacccc ctcaagccgc cgccgtcgcc gaagacgcga gtgccgaggc
     8101 gcgagccgga cgggaactgc catgcccggt gctgccaggc ctgggtgtcg agatcggccg
     8161 agacgccggt ggcgaagttc accggcagcg ccgcggactc ggcgcccgcg atgttctgga
     8221 ccgcgtccga cctccagccg gcggcggtcc tgcggatcac gaccatgtcg tcctggtgcg
     8281 ggaacgccgt gcccgcctcg cgatcgagga tgccgaagcg caggccgaac agcagcgact
     8341 cgccgtccgc agacggcgtt ccgggattgg ccgcggcatg gtcgggcatc gactcgccac
     8401 cgcgacggtt gttcttgtcg ggagtcgtga ccatctcgta cgctcgaccc ggtggcggag
     8461 cgaccgcacc gcgcgtggtg aaggtgcggt cctcaccctc ggaggtgcct atcgagttgg
     8521 tcgccacgag gcggtagtgg tacgtcgtgg cgggcgtcag cccgctggcg tcctgcgcca
     8581 cggcgcgcac gatgccggcg gagccggcat ccgccgccgg gatcgggatc cgcgacccgt
     8641 agtcggcgct cgtacccagc tcgaagtagt aggtggtggg gaggttgttc gggttcaccc
     8701 gcccgagcag ccgcgccgag gtcgctgcga cgtgctgcga cgctccggtg gtgacatccg
     8761 gaggcgagac cggcgtggtg accgtcgtct cggccgacac ggccgtcggt ccgccgaacc
     8821 gcttcgtcgc gaccagccgc acccgataga tggagttcgc ctcgagcgtg ctgacggtct
     8881 cggtcaccgt gacgggatcg tcgccggtca ccacgccccc cgacccggcg tcgatccagg
     8941 tgactccgtc cctgctgtac tcgaaccggt acgaggccgc gccggtgttc gaatcgacgg
     9001 tgccgtgcag cgtcgcccca tgggcggtca ccccggttgc cggagcgatg gtgagcgctg
     9061 gctgcggggc tccgtcgtcg tcgaggatca gcacgacatt gtgggtgccg ttggtcacgt
     9121 agatgtcacc ggtcgagcct gagaccgcga gaccgcgcat cgagacggcg tccaggcccg
     9181 cgtcggccaa gtgggtgtcc acgagcgcgc ccgccgagtc gagctccttg atgcgcgcct
     9241 cgccggcggc gctgtcgtgc tgtccgacga ggacatggtc cgtggaggga tcgacggcga
     9301 gcaccgtggg cgactggggg atgacgtccg cgccgaagac ggtcgccgtc gcgccgcttg
     9361 ggtcgaagcg caggatccgc ccgttcgcgt gatcgagggc gtagatgtcg ccgctggcgt
     9421 cgacggccaa ggccacagga gaggtggacc cgaactggcc gacaccggat cccgagatgc
     9481 cctcctgaca cccgctcgcg gcggtgcagg tctcgagtgc ggcggcgcct gtatccactc
     9541 cccagccgaa cgcgcgcagg aagccgcccg aagagtcgaa gcgctggatg cggcgattcc
     9601 cgggatcggt gacgagcacg tctccggtcg cctggtcgac ctccacgtcg gtgcgattcg
     9661 cgccggtgag ctgtccggct ccggacccgg acacgcctcg ctggcaggtc gtcgcgatag
     9721 tgcagatctc tagggccgcc gcgcccgtgt cgacgcccca gccaaatgcg aagaggaagt
     9781 tcccgtctgc atcgaacttg ctcacgcgac ggttgccgcc ctccgcaacg tagaccgaac
     9841 cgtcgtcctc gcttatagcc aagccggacg ggagaaagag ctgtccggcg gccgcaccgc
     9901 tctcaccgcg ctgacagttc gcggccaccg tgcaaatctc gaaccccgtg ccgcctgaga
     9961 cgtcgacgtt ctgtccccat gtcagctcga aatcatctgc agcgctaaag cgctgaatcc
    10021 gctcgaccgc gtagtcgctg acataggcgt cgcctgtcac ctggttcagg gcaacgtccc
    10081 ccacgccctg aaactcaccg ccgctcgtcc cgggaccgcc gaagaaggag tccaccgtct
    10141 tgccggcgtg cgcgggagcc gccatcacgg cgagaccgag cgcggcgacg acgatgacca
    10201 gccacgacaa ggatccgatc cggcgctgcg agcctctgta tctcatatcc ataccggacc
    10261 gtccttcagc tgttggtgtg tttgccatcg ccatcactcc caccacccac cgcctccgcc
    10321 atcagcagct cgtgcggctg ctgatggcgg agg
//
LOCUS       DATABV010000162         6514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_532287, whole genome shotgun sequence.
ACCESSION   DATABV010000162 DATABV010000000
VERSION     DATABV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532287"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VHF88_07090"
     CDS             <1..285
                     /locus_tag="VHF88_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX2161570.1"
                     /translation="ITPPELSGGTFSVSNLGMYGIERFTAIVNPPQAAILTVGALMKK
                     PAVDDRGRVVARDLMTLTLVCDHRILYGADGAEFLAQIRELLEQPLSLAL"
     gene            complement(290..817)
                     /locus_tag="VHF88_07095"
     CDS             complement(290..817)
                     /locus_tag="VHF88_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX2161571.1"
                     /translation="MPAEQFVSELNDQIGREYGAHQQYVAVAVYYDALTLPQLADFFY
                     RQAIEERGHAMMMVQYLLDAGERPRIPGVAEPRNDFADVVEPIRIALEQEKRVTDQIS
                     GLAAIAHDAHDYVSGQFMQWFLKEQVEEVATMSDLLAIAERAGDRWSEIEDFVAREHP
                     GDEGGDPTAPTPAGG"
     gene            893..2311
                     /locus_tag="VHF88_07100"
     CDS             893..2311
                     /locus_tag="VHF88_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX2161572.1"
                     /translation="MGAPLILGAIETDYLIVGAGATGLAFADTLVAEDAEVEVTVVDR
                     RPASGGHWLDAYPFVRLHSPSAYYGVGSLPLGEDRIDRDGENARFYERATGAEVRDYF
                     AEVAARLERSGRVRLLSEHEHLDQDGDAERVRDLGTGELHDVAVRRSVVDARYLEASV
                     PSTHIRSFEVVAGTRVVPLNDLPEIAESASCYAVLGSGKTAVDACTWLLDNGVEADRI
                     RWVRPRDAWFDDRAYFQPLDLVGKIMEGIALDTEAGAQATDLDDLFDRLEASGRLWRI
                     DPSQPATMYRGTMLSARELEAVRQIEDVVRLGHVRRVEADRILLERGEVDTGPGVLHV
                     DCTALGLRNAPATPIFQPGRIVLQQVRHNSPSFNAALLAFVEAHRDDDDAKNRLCPPN
                     PYSSGIADWPRMMTRTWRTERRWLNEPDLAAWLVESRLNLLGALPAHAGERSVRSAVE
                     RYLANVGPAIERLARLDERSAS"
     gene            2451..2705
                     /locus_tag="VHF88_07105"
     CDS             2451..2705
                     /locus_tag="VHF88_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161573.1"
                     /translation="MTAKEKLRAQVDELSEAEAASARIILERTANRDEIGEAIAAGYR
                     RVPQATPDEWGPLFKLTESTTAQTFQRLDAEERAAGLDPW"
     gene            complement(3120..4190)
                     /locus_tag="VHF88_07110"
     CDS             complement(3120..4190)
                     /locus_tag="VHF88_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161574.1"
                     /translation="MATYDLLRELPLTIESYELEGLAQRFSPAMERLTTEIVLRGGGE
                     EGRGEDVTYESRDQEAFQAAGPVHDLAGEHTLGSFAALLDGLDLFPQPPDREDYRDYR
                     RWAFESAALDLALLQAGRSLGDVLGREPRPLNYVVSMRLAPMGQEGQETVDRLLRVLE
                     LYPSTRFKLDPTNGWTPELIAQLVETGAVDSLDLKGRYKGTPVDVETDPELYRAVAEA
                     FPDAWLEDPDLDSAEAMAVLEPHRDRITWDAPIHSVADIEALPFPPKTVNVKPSRFGP
                     LERLFAAYDYCEAHGIGAYGGGQSELGVGRDQIQYLAALMHPTTPNDTAPKEFNLPEP
                     PPGLPESPIDLPIAPTGFRFER"
     gene            complement(4204..4704)
                     /locus_tag="VHF88_07115"
     CDS             complement(4204..4704)
                     /locus_tag="VHF88_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161575.1"
                     /translation="MPAEFRPRGLLEHLTRHGVDFVVVGGVAAALHGSERNTFDLDIC
                     PAQDADNLEALGTALTEIDARLRGIEEDDVPFVPDARSLAGIEILTLSTSLGSLDILV
                     RPHGSPSYAALSRRATRLDVGSSPVLVASLDDLIAMKRQSDRPKDREDVERLETIARL
                     TRRLAR"
     gene            complement(4694..5020)
                     /locus_tag="VHF88_07120"
     CDS             complement(4694..5020)
                     /locus_tag="VHF88_07120"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2161576.1"
                     /translation="MHAAAQLIRDARTSSGLTQAELGRRLGVTQAAVAQLERPDANPS
                     VARLDEVLRALGQRLALAAQPVTGDIDETLIARNLRMSPSERLDAFETAHRELAELRS
                     LMPDAG"
     gene            complement(5046..5912)
                     /locus_tag="VHF88_07125"
     CDS             complement(5046..5912)
                     /locus_tag="VHF88_07125"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2161577.1"
                     /translation="MSGPWSWLRRNALLLYAAVAVLYMLLPIIVIGLFSFDQTSTGKL
                     DYRWSGLTLDYWREAFADESVNEAMITSIQLAGLSMLVSTVIGTLMALALVRHQFRGR
                     KASNLLIVLPMATPEIVIGAALLSMFLIYGLQLGFTTLFIAHVMFSISFVVVVVRSRL
                     IGFDRSLEEAAADLGAPPLTTFRTITLPLLAPGIVAAALLAFALSIDDFVISNFNSGT
                     TETFPLYIFGVSLRGIPVQVNVLATLLFLVTVVAIALVLWQQRRAERMAEVRPEPEPQ
                     PAVATTGEQAIA"
     gene            complement(5909..>6514)
                     /locus_tag="VHF88_07130"
     CDS             complement(5909..>6514)
                     /locus_tag="VHF88_07130"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2161578.1"
                     /translation="AIALRAGRWKLLLLFAVVAPFFTTYLIRTIAWKTILADASPAVD
                     VLQVLQLVPQDGRVLATGGAVVAGLTYNFLPFMILPIYASLERMDVRLIEAAKDLYAS
                     SAKAFLKVTLPISAPGIVAGILLTFIPAVGDYVNALLLGGTGQSMVGNVIQSLYLQQR
                     DYPEAAALSFVMMLAILAVVVVYLRLAGTGAFIGDEEEERA"
BASE COUNT         1039 a         2214 c         2311 g          950 t
ORIGIN      
        1 atcacgccgc cggagctctc aggcggcacc ttcagcgtct cgaacctcgg catgtacggg
       61 atcgagcgct tcaccgcgat cgtcaacccg ccgcaggcgg cgatcctgac cgtcggcgcg
      121 ctgatgaaga agccggcggt cgacgaccgc ggccgtgtcg tcgcccgcga cctgatgacg
      181 ctcacgctcg tctgcgacca ccgcatcctc tacggcgccg acggcgccga gttcctcgcc
      241 cagatccgcg agctgctcga gcagccgctc tcgctggcgc tctagccggc tatccgccgg
      301 cgggcgtcgg cgcggtcggg tcgccgccct cgtcgccggg gtgctcgcgt gcgacgaagt
      361 cctcgatctc cgaccagcgg tcgcccgcgc gctcggcgat cgcaagcagg tcggacatcg
      421 tcgcgacctc ctcgacctgc tccttgagga accactgcat gaactggccg ctgacgtagt
      481 cgtgcgcgtc gtgggcgatc gccgcgaggc cggagatctg gtcggtgacg cgcttctcct
      541 gctcgagggc gatcctgatc ggctcgacga cgtcggcgaa gtcgttgcgc ggctcggcga
      601 cgccggggat ccgcggccgc tcgccggcgt cgagcaggta ctggaccatc atcatcgcgt
      661 ggccgcgctc ctcaatcgcc tggcggtaga agaagtcggc gagctggggc agcgtcagcg
      721 cgtcgtagta gacggcgacc gcgacgtact gctggtgagc gccgtactca cgcccgatct
      781 ggtcgttgag ctccgatacg aactgctccg cgggcatcct cggcctcctg gtcgctggct
      841 gactcgcggc gacgctagcg gacggtgcga gttcggtttc ccttcctgac gattgggagc
      901 gccccttatc cttggcgcca tcgagaccga ctacctgatc gtcggagctg gggcgaccgg
      961 gctggccttc gccgacacgc tggtggcgga ggacgccgag gtcgaggtga cggtggtcga
     1021 ccgccggccc gcctcggggg gccactggct ggacgcctac ccgttcgtcc gcctgcactc
     1081 gccgtcggcg tactacgggg tgggctcgct gccgctcggc gaggaccgca tagaccggga
     1141 cggcgagaac gcccgcttct acgagcgcgc gacgggcgca gaggtgcgcg actacttcgc
     1201 cgaggtcgcg gcgcggctcg agcggagcgg ccgggtccgc ctgctcagcg agcacgagca
     1261 cctggaccag gacggcgacg ccgagcgcgt ccgcgacctg ggcacgggag agctccacga
     1321 cgtcgcggtc cgccgcagcg tggtcgacgc ccgctacctg gaggcgtccg tgccttccac
     1381 gcacatccgg tcgttcgagg tggtcgccgg cacgcgcgtg gtgccgctca acgacctccc
     1441 ggagatcgcg gagtcggcct cctgctacgc cgtgctcggc tccggcaaga ccgcggtcga
     1501 cgcgtgcacc tggcttctgg acaacggtgt ggaggcggac cgcatccgct gggtccgacc
     1561 ccgcgacgcc tggttcgacg accgcgccta cttccagccg ctcgacctgg tcggcaagat
     1621 catggagggg atagcactcg acaccgaggc cggcgcccag gccaccgacc tcgacgacct
     1681 gttcgaccga ctcgaggcct ccggccgcct ctggcgcatc gacccgtcgc agcccgcgac
     1741 gatgtaccgc ggcacgatgc tcagcgcgcg cgagctcgag gccgtgcggc agatcgagga
     1801 cgtcgtcagg ctcgggcacg tgcggcgcgt cgaggccgac cggatcctgc tcgagcgcgg
     1861 cgaggtcgac accggccccg gcgtcctgca cgtcgactgc accgcgctcg gcctgcgcaa
     1921 cgcgcccgcc acgccgatct tccaaccggg ccggatcgtc ctccagcagg ttcggcacaa
     1981 ctcgccgagc ttcaacgcgg cgctactcgc cttcgtcgag gcccaccgcg acgacgacga
     2041 cgccaagaac cggctctgcc cgcccaatcc gtattccagc ggcatcgccg actggccgcg
     2101 catgatgacc cgcacctgga gaacggagcg gcgctggctg aacgagcccg atctcgcggc
     2161 ctggctggtc gagagccggc tgaacctcct cggcgcgctc cccgcccacg ccggcgagcg
     2221 ctcggtgcgg tcggccgtgg agcgctacct cgccaacgtg ggaccggcga tcgagcggct
     2281 ggcccggctc gacgagcgtt cggcttcgta gagcgcggtc cttagaccga gaacgcccat
     2341 cggcggcggc tatagacctg catgcgacca cgggtagcga gggagacgca cgcggcatcg
     2401 ttacgcaaac cggctttcgc agcccacgga cggcggtaga atcggacccg atgacggcca
     2461 aggagaagct acgcgcccag gtcgacgagc tgtcagaggc cgaggccgcg tcggcgcgca
     2521 tcatcctaga gcgcaccgcg aatcgcgacg agatcggtga ggcgatagcg gccggctaca
     2581 ggcgcgtccc acaggcgacc cctgacgagt ggggccccct gttcaagctc accgagtcca
     2641 cgacggcgca gacattccag cgcttggacg ccgaggagcg tgctgccggc ctcgacccgt
     2701 ggtgaaacac ggcgaggtct ggtggtctga gcatcccacc gccggccgtc ggccggtcct
     2761 ggtcttgacc cggaccgaga cgatcaacgt cctcaacgag atcctcgtcg tgccggccac
     2821 acgaacggtg cgtggtattc ccaccgaggt tgaactggac cagagcgatg gaatgccaac
     2881 cacgtgtgtc ttgtccctag acaatctgac cccactcccc aaaggtctac tgatagagcg
     2941 aatcgccgcc ctcaacgcag acaagcttcg ggaagtctgc actgctctgg gtgccgcggt
     3001 cgactgctga agtatcagtc gttttcgcaa agagcgggag cttgaaaaag tccgcgagac
     3061 gtacgttcgt gccgagagga ttcgaacctc tgaccttggg tccgtagtcc tgctgccggc
     3121 taccgctcga agcggaagcc ggtgggggcg atcggtaggt cgatcgggct ctcggggagg
     3181 ccgggcggcg gctcggggag gttgaactcc ttcggcgcgg tgtcgttcgg cgtggtgggg
     3241 tgcatcaggg cggcgaggta ctggatctgg tcgcggccga cgccgagctc ggactggccg
     3301 ccgccgtagg cgccgatgcc gtgcgcctcg cagtagtcgt aggcggcgaa gaggcgctcg
     3361 agcgggccga agcgcgacgg cttgacgttg acggtcttcg gcgggaaggg cagcgcctcg
     3421 atgtcggcga ccgaatggat cggggcgtcc caggtgatcc ggtcgcggtg cggctcgagc
     3481 accgccatcg cctcggcgct gtcgaggtcg gggtcctcga gccaggcgtc ggggaacgcc
     3541 tcggcgacgg cgcggtagag ctcggggtcg gtctcgacgt cgaccggcgt gcccttgtag
     3601 cggcccttga ggtcgagcga gtcgaccgcg ccggtctcga ccagctgcgc gatcagctcg
     3661 ggcgtccagc cgttggtcgg gtcgagcttg aagcgcgtgc tcgggtagag ctcgagcacg
     3721 cgcagcaggc ggtcgacggt ctcctgcccc tcctgaccca tcggcgccag acgcatcgag
     3781 acgacgtagt tcagcggtcg cggctcgcgg ccgagcacgt cgccgagcga gcggccagcc
     3841 tgcagcagcg cgaggtcgag cgccgccgac tcgaacgccc agcggcggta gtcgcggtag
     3901 tcctcgcggt ccggcggctg cgggaacagg tcgagcccgt cgagcagcgc cgcgaacgag
     3961 ccgagcgtgt gctcgccggc gaggtcgtgg accggcccgg ccgcctggaa cgcctcctgg
     4021 tcgcgtgact cgtaggtgac gtcctcgcca cgcccctcct cgcctccgcc gcgcagcacg
     4081 atctcggtcg tcaaccgctc catcgccggc gagaaccgtt gcgccaggcc ctccagctcg
     4141 tacgactcga tcgtcagcgg cagctcgcgc agcaggtcgt aggtagccat ccggccatcc
     4201 tattcagcgc gcgagccggc gagtgagcct tgcgatcgtc tcgagccgct cgacgtcctc
     4261 gcggtccttc ggtcggtccg actgacgctt catcgcgatc aggtcgtcca gcgaagcgac
     4321 gaggaccgga gaggacccga cgtcgagtcg agtcgctcgc cggctgagcg cggcgtacga
     4381 gggactgcca tgcggacgga ccaggatgtc gagcgacccg agcgacgtcg agagcgtgag
     4441 gatctcgatc ccggcgagcg aacgagcgtc cgggacgaac gggacgtcgt cctcctcgat
     4501 gccacgcagc ctggcgtcta tctcggtcaa cgccgtcccg agcgcctcga ggttgtcggc
     4561 gtcctgtgct ggacagatgt ccaggtcgaa ggtgttgcgc tcggagccgt ggagcgccgc
     4621 ggcgacaccg ccgacgacga cgaagtcgac gccatgccga gtcaggtgct cgagcagccc
     4681 gcgcgggcgg aactcagccg gcatcgggca tcagcgaacg caactcggcc agctcgcgat
     4741 gagcggtctc gaaggcgtcc aagcgctcgc tcggcgacat gcgcaggttg cgcgcgatca
     4801 atgtctcgtc gatgtcgccc gtcaccggtt gcgcagcgag cgccagtcgc tggcccaagg
     4861 cgcgcaggac ctcgtcgagc cgagcgacag acgggttcgc gtccggtcgc tctagctggg
     4921 cgactgcggc ctgcgtgacg ccgagccggc gaccgagctc cgcctgggtc aggccgctcg
     4981 acgtacgggc gtcacggatc agctgagctg cggcatgcac agctcaacat tagcaatgac
     5041 ttatgtcagg cgatcgcctg ctcgccggtg gtggcgacgg ccggctgggg ctcgggctcg
     5101 gggcggacct cggccatgcg ctcggcgcgg cgctgctgcc agaggacgag ggcgatggcg
     5161 acgacagtga cgaggaagag cagggtcgcg aggacgttga cctggaccgg gatgccgcgc
     5221 aggctgacgc cgaagatgta gagcgggaag gtctcggtgg tgccggagtt gaagttcgag
     5281 atgacgaagt cgtcgatcga gagggcgaag gccagcagcg cggcggcgac gattccgggc
     5341 gcaagcagcg gcagggtgat cgtgcggaag gtggtcagcg gcggggcgcc gaggtcggcg
     5401 gcggcctcct cgaggctgcg gtcgaagccg atcaggcgcg agcgcacgac gaccacgacg
     5461 aagctgatcg agaacatgac gtgggcgatg aacagggtcg tgaagccgag ctgcagcccg
     5521 tagatcagga acatcgacag cagcgccgcg ccgatcacga tctccggtgt cgccatcggc
     5581 aggacgatca gcaggttcga cgccttgcgg ccgcggaact ggtggcggac cagcgccagc
     5641 gccatcagcg tgccgatgac cgtcgagacg agcatcgaca ggccggccag ctggatgctg
     5701 gtgatcatcg cctcgttgac cgactcgtcc gcgaacgcct cgcgccagta gtcgagcgtc
     5761 aggccgctcc agcgatagtc gagcttgccg gtcgaggtct ggtcgaacga gaagagcccg
     5821 atcacgatga tcggcagcag catgtagagg accgcgaccg ccgcatagag cagcagcgcg
     5881 ttgcgacgca gccagctcca gggaccgctc acgcgcgctc ctcctcctcg tcgccgatga
     5941 aggcgcccgt gcccgccagt cgcaggtaga cgacgaccac cgcaaggatc gccagcatca
     6001 tcacgaacga gagcgccgcc gcctcggggt agtcgcgctg ctggaggtag agcgactgga
     6061 tcacgttgcc gaccatcgac tggccggtgc cgccgagcag cagcgcgttg acgtagtcgc
     6121 cgacggcggg gatgaacgtc agcaggatgc cggcgacgat cccgggcgcg ctgatcggca
     6181 gcgtcacctt caggaacgcc ttcgcgctcg acgcgtagag atccttcgcc gcctcgatca
     6241 gccgcacgtc catccgctcg agcgacgcgt agatcggcag gatcatgaac ggcaggaagt
     6301 tgtaggtcag gcccgccacc accgcgccgc cggtcgccag cacgcggccg tcctgcggga
     6361 cgagctgcag gacctgcagg acgtcgaccg ccggactggc gtcggcgagg atcgtcttcc
     6421 aggcgatcgt gcggatcagg tacgtcgtga agaacggggc gacgaccgcg aacagcagca
     6481 gcagcttcca gcgaccggcg cgcagcgcga tcgc
//
LOCUS       DATABV010000163         9011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2220540, whole genome shotgun sequence.
ACCESSION   DATABV010000163 DATABV010000000
VERSION     DATABV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220540"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VHF88_07135"
     CDS             complement(<1..1206)
                     /locus_tag="VHF88_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX2161579.1"
                     /translation="MFQEGTALESLESVDSRRFLPHALVATLLVIVAPALVVLTLSPL
                     SGIADLVLSAVLATGLSVAAGTVGSAAWTRLAQSREIPFADLMLWAWARRVRAERRLA
                     KAAAGGAPLGPGGELTGLRTLSVVFEARDGFSHGHSGRVARHAGRIARRMGLSSDEVE
                     RIAAAAAVHDVGMLGVPRAVLAKDELSPEERRLVERHAVAGADQVAAVAGPETAALVR
                     HHHERVDGSGYPDGLSGDEIPLGARIIAVADRFDELVSPTPGRPGSTCGNALDELSAR
                     AGSELDPAVVAAFVGYYSGVRSIAGVALAATAPQRAVRWIAAAPATIGSAAGAPVVLQ
                     GACVAGTAALAGACIGGLPALSPDREDRPTAKEQPERGLAAKGRHAAGPSLAASGSAG
                     GDHARGKDRR"
     gene            complement(1240..1312)
                     /locus_tag="VHF88_07140"
     tRNA            complement(1240..1312)
                     /locus_tag="VHF88_07140"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1277..1279),aa:His,seq:gtg)
     gene            complement(1369..2061)
                     /locus_tag="VHF88_07145"
     CDS             complement(1369..2061)
                     /locus_tag="VHF88_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161580.1"
                     /translation="MVRELVALGVAAAVALAAPALAHAAGEYEPNDSQRMAFGPLVNG
                     QVLTAGLESLEDTDVYLFYVAGPSSVRFRIQNNFTPGADACGYVLRIFEAGEQFAVEQ
                     QDFVSGQCLKAETDGDMFKSLEAGKYYLTIDEPLGWGIDVETSTYTLTAVSGLSSLAT
                     MQAGCDTRKAAAKRAKKAMTRATAALRTAEKQGSGVKSAKRKLAKARTARRKSARAAK
                     SYCAIRQRAPAA"
     gene            complement(2174..3847)
                     /locus_tag="VHF88_07150"
     CDS             complement(2174..3847)
                     /locus_tag="VHF88_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2161581.1"
                     /translation="MSGRLTRRGLLGRGGAVAGSAALGGLAAGALSATAAEAQQGEDG
                     PNVLLIVASGIRADSVGAFDGAFDDNEVAETPNLDDLAGDSLRFERAVPEAMPAVISR
                     RALVTGKRSFPYREWRRHEGYAPVPGWNPVYVRQPLIAEALARENVETAWITDNPFFS
                     GPRFDGWVRRTTDVDAADYPAPPRIQGTLGREQTRREVERYLVGGIERSAQAMNRAVR
                     VGERQLTRLARGGRQFFLAVDAFDPAEAYYAPRSWTRTLDVDPSLAAPLKGRDWIVPA
                     DIGDDTVAIARDAYMRGVEQVDGAVGRLLAKLDDLDLTDDTIVWFVSDGGTMLGEHGW
                     LGRGAPGSYKHAYFVPFLLRDPRGRRSGDYSYYYASTHDVAPTLLSMMGIDRPGRMDG
                     EDLTALLDDEDPPKRRAFMCASVTKIAVGDPRWLMLMDMQGDERELHDFDNENSDNED
                     DDEENVVRKHPPVVAELEKVPLAAAQGTYPRFDDESAVRPPPEDDDKDDDGIDDNDET
                     NDETSGETRDADGDDLDFDESQGRDPNAAVLPDSNPPPATTTTAPTSSP"
     gene            complement(3844..5379)
                     /locus_tag="VHF88_07155"
     CDS             complement(3844..5379)
                     /locus_tag="VHF88_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2161582.1"
                     /translation="MALQPRLTRRRALQAGGAGALALGLGACNDDLEPQISHGEDAPN
                     VVLIVTDSTRRDFVSAYDDGDELADTPNLAALAKESLLFDHAVPEAMPTGTVRRALIT
                     GMRSFPFRNWQVTPHLPAEPGWNDIYPYQPMFTEVMSEAGVETAYVTDNPFLVGPRFV
                     NFRRTLNMARPDYSQASYRAFNRPFKRPAPRSAIERYLFPKLSDSIEVQRLREYVGWN
                     NLYRRRESDYSAARVIRSGMDCLDELKDKRPFFLGVDAFDPHESLDPPRAYMTKFARV
                     KGSEREGIIPIQPWDTPYSRVFDLGIDQETLERVRELYAAELTFVDRWIGRLLNKMDD
                     LGLMDNTAVVYLSDHGLTLGEYGLLGKHAAYPNWPIYRVPYMIREPGGRKLAGQRSKY
                     FASTHDVASTLLGFQGIRAPGLMDGEDLSRLFDGKRPYEERPYFTACYARYLICGDGR
                     WVLIAHSEKRRIRLFDTLTDPGETINLAQQNPAKVEELWAVLEQEAGGTLPQFGDETV
                     MGG"
     gene            5470..6348
                     /locus_tag="VHF88_07160"
     CDS             5470..6348
                     /locus_tag="VHF88_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2161583.1"
                     /translation="MTAPTFDLQSHSIHSDGELPTAEVVANAAAAGVELLALSDHDTV
                     DGVDEALAAGREHGVRVVPATEISAVDGAYEDMHVLGYGVDQSDPVLAERLLDARGDR
                     ERRAEAMSDRLQELGFEIDPAPVDARRAAGKPVGRPHLAAGVLAHPANAERLAAEGHA
                     DVSSFIPAYLIQGTPGYVARSHPTVAEAIGWIHDAGGIAIWAHPFWDVEDDGEVLALV
                     DRFKEAGLEGVEVFYVTHTQAQVELLADRCSELGLLATGSSDYHGPDHRLFSSFRAHQ
                     THGIEPNLGPIPELAS"
     gene            complement(6353..7018)
                     /locus_tag="VHF88_07165"
     CDS             complement(6353..7018)
                     /locus_tag="VHF88_07165"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2161584.1"
                     /translation="MKLIGVGFGRTGTMSLKGALEQLGADPCFHMIDLIMGENKERDL
                     AYWKKIADGEPVDWHEVFDGWEATVDWPAAARWREIVDAFPDVPVLLNYRDFDGFYKS
                     CQNTILAVKQAAMAGELTQDASREPPSPVLWEVIEKLIWQGDFQGRFEDKEWVRQMYY
                     DRIEEIKAYVDSDRLIVWELGKDGWEPLADALGVAVPDEPFPHLHDTNEFRAEFGLQP
                     VAA"
     gene            complement(7084..8778)
                     /locus_tag="VHF88_07170"
     CDS             complement(7084..8778)
                     /locus_tag="VHF88_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2161585.1"
                     /translation="MAKRRENPPVEQLYAKRHKIFAVMMIGWAMSLLDVSIVNVAVPE
                     LQQELDTDVNSITWVVNSYNLSFAALLIAAGKLADQFGRRRFFILGMGVFTLGSLFCA
                     ISPSADVLILSRVIQGAGAGILAPLGFAITAAVFPIEERGKALATIAIVALAASASGP
                     AIGGAIVELASWEWIFIVNLPLGVLGIVLARRIWPETYDLDADTRIDYLGMTLLALAV
                     FCLTFGLVDANHRGWTDGLTLFLLQSSILLGIAFVLSQRRDGAMITRSLQSNKQFVGA
                     NVAMLLFAAGALGALLLMALTFVNLWGFTIFEAALALMPVPVTGILVWPIVGKAADNR
                     PPRDLAAPALGLMAVGLLWVSFTPALWDGWVDYLIVFPGLVLIGIGMGTGFPTINVGA
                     MGAVQGQELGLASGILNTSRQLGAAIGIALLVATFSMTLTWHGQSAVDDVADLAQEYQ
                     VPPAVFAGLMQRDMKVFAGGSTDRTDPGPGFDEQVFRRTAGAARNSFGWAYRAAMLLI
                     LLAIPFTLTMRRSPAMARAAAMAAMQQAQEAQQDGGPRAGPGPTIRRGPAGTQPAG"
BASE COUNT         1317 a         3213 c         3167 g         1314 t
ORIGIN      
        1 gcggcgatcc ttcccacgcg cgtggtcgcc gccggccgag ccggacgcgg ccaacgacgg
       61 tccggccgca tgccgaccct tggccgcgag gccgcgctcc ggctgctcct tcgcggttgg
      121 ccggtcctcc cggtcgggcg acagggccgg caggccgccg atgcaggcgc cggccagggc
      181 cgccgtcccg gcgacgcacg ccccctgcag gacgaccggg gctccggcgg ccgagccgat
      241 cgtcgccgga gccgccgcga tccagcgcac cgcacgctgc ggcgcggtgg cggccagcgc
      301 gacgccggcg atcgaccgaa cgccggagta gtagccgacg aaggcggcaa ccacagcggg
      361 gtccagctcg ctcccggcgc gtgcggagag ctcgtcgagc gcgttgccgc aagtgctccc
      421 cggacgtccg ggagttgggg acacgagctc gtcgaagcgg tccgcgacgg cgattatccg
      481 cgcaccgagc gggatctcat caccggacag accgtcgggg tagccgctgc catcgacccg
      541 ctcgtggtga tggcgtacca gcgctgcggt ctcgggaccc gcgaccgccg cgacctggtc
      601 ggcgccggcg acggcatggc gctcgacgag ccgccgctcc tccgggctca gctcgtcctt
      661 ggcgagcacc gcgcgcggta cgcccagcat tccgacgtcg tgcaccgccg ccgccgctgc
      721 gatgcgctcg acctcgtcgc tcgacagacc catccgccgc gcgatccgac ccgcatggcg
      781 ggcgacgcgg ccgctgtggc cgtggctgaa tccgtcacgg gcctcgaaga cgaccgagag
      841 cgtgcgcagg ccggtcagct ctcccccggg cccgagcggc gcgccgccgg ccgccgcctt
      901 cgccagccgt cgctcggcgc gcacgcggcg cgcccaggcc cacagcatca ggtccgcgaa
      961 cgggatctcg cgcgattgcg ccagccgcgt ccacgctgcc gatcccaccg tgcccgccgc
     1021 gaccgacagg cccgtggcga gcaccgccga gagcaccagg tcggcgatcc ccgacagcgg
     1081 cgacagcgtc agcaccacca gcgcgggcgc gacgatcacc agcagcgtag cgaccagcgc
     1141 gtgcgggagg aaccgccggg agtcgacgct ctccagggac tcgagtgctg tcccctcctg
     1201 gaacaccctg cgtcaagcgt accgccggac cggcagaggg gcgaacgacg ggactcgaac
     1261 ccgcgaccgc tcggaccaca accgagagct ctaccagctg agctacgtcc gccaggacgc
     1321 agccgagact accaacgatg cgggttcgga caaagagtcg gagcgctgct aggcggcagg
     1381 cgctcgctgc cggatggcgc agtaggactt ggcggctcgg gcgctcttcc tgcgcgctgt
     1441 cctggccttg gccagcttgc gcttggcgct cttgacgccc gacccctgct tctctgccgt
     1501 gcgcagcgct gccgtggccc gcgtcatggc cttcttcgcg cgcttggcag ccgccttcct
     1561 ggtgtcgcat cctgcctgca tcgtcgcgag cgaactgagt ccgctgacgg ccgtgagcgt
     1621 gtaggtgctc gtctcgacat cgatgcccca gcccaggggc tcgtcgatcg tgaggtagta
     1681 cttcccggcc tccagcgact tgaacatgtc gccgtctgtc tcagccttca gacactgacc
     1741 agagacgaaa tcctgctgct cgacggcgaa ctgctctccg gcctcgaaga tccgaagcac
     1801 gtatccgcaa gcgtccgcgc caggcgtgaa gttgttctgg atgcggaagc gaacgctcga
     1861 cgggcccgct acgtagaaga gatacacgtc ggtgtcctcg agcgactcca accccgccgt
     1921 gagtacctgg ccgttgacta gcggcccgaa agccatccgc tgagagtcgt tgggctcgta
     1981 ctcgccggcc gcgtgagcta gcgcaggcgc agcgagcgcc acggccgcgg cgacccccag
     2041 cgccacaagc tcccgaacca ccgatcgcga acccgcgtgc atgccccctg cgctcatgaa
     2101 ttcctcttct cgacggccga agtagcgaca acagcctaac ggtgcatacg ccagcccgca
     2161 ttgcggcctc acgctacggg ctcgaggtcg gagcggtcgt cgtggtggcc ggcggcggat
     2221 tggagtcggg gagcaccgcc gcgttcggat cgcggccctg gctctcgtcg aagtcgaggt
     2281 cgtcgccgtc ggcgtcgcgc gtctcgcccg aggtctcgtc gttggtctca tcgttgtcgt
     2341 cgatgccgtc gtcgtccttg tcgtcgtcct cgggcggcgg ccggacggcg gactcgtcgt
     2401 cgaagcgggg ataggtgccc tgggcggccg cgagcgggac cttctcgagc tcggcgacga
     2461 ccggcgggtg cttgcgcacg acgttctcct cgtcgtcgtc ctcgttgtcg gagttctcat
     2521 tgtcgaagtc gtgcagctcg cgctcgtcgc cctgcatgtc catcagcatc agccagcgcg
     2581 ggtcgccgac ggcgatcttg gtcaccgacg cgcacatgaa cgcgcgccgc ttcggcgggt
     2641 cctcgtcgtc gagcagtgca gtcaggtcct ccccgtccat cctgccgggg cggtcgatgc
     2701 ccatcatcga cagcagggtc ggcgccacgt cgtgggtcga ggcgtagtag tagctgtagt
     2761 cgccggagcg gcgcccgcgc ggatcacgca gcaggaacgg cacgaagtag gcatgcttgt
     2821 agctgccggg agcgccccgg ccgagccagc cgtgctcgcc gagcatcgtc ccgccgtcgc
     2881 tgacgaacca gacgatcgtg tcgtcggtga ggtcgaggtc gtcgagcttg gccagcagcc
     2941 gtccgacggc gccgtcgacc tgctcgacgc cgcgcatgta cgcgtcgcgg gcgatcgcca
     3001 cggtgtcgtc gccgatgtcg gccgggacga tccagtcgcg acccttcaag ggtgccgcca
     3061 ggctcgggtc gacgtcgagg gtgcgcgtcc agctgcgcgg cgcgtagtag gcctcggccg
     3121 ggtcgaacgc gtcgacggcg aggaagaact gccgaccacc gcgtgcgagc cgcgtcagct
     3181 ggcgctcccc caccctgacg gcgcgattca tcgcctgcgc gctgcgctcg attccgccga
     3241 cgagatagcg ctcgacctcg cggcgggtct gctcgcgacc gagcgtccct tggatccgcg
     3301 gcggcgccgg atagtcggcg gcgtcgacgt cggtcgtgcg acgcacccag ccgtcgaagc
     3361 gcgggcccga gaagaacggg ttgtcggtga tccaggccgt ctcgacgttc tcgcgggcga
     3421 gcgcctcggc gatcagcggc tggcggacgt agacggggtt ccagccggga accggcgcat
     3481 acccctcgtg gcggcgccac tcgcggtagg ggaacgaccg cttgccggtg accagcgcgc
     3541 ggcgcgagat caccgcgggc atcgcctcgg ggaccgcgcg ctcgaagcgc agcgagtcac
     3601 ccgcgaggtc gtcgagattc ggcgtctcgg cgacctcgtt gtcgtcgaac gccccgtcga
     3661 acgcgccgac gctgtcggcg cggatgccgc tcgcgacgat cagcaggacg ttcggaccgt
     3721 cctcgccctg ctgggcctcg gcggcggtcg cgctcagcgc tccggcggct agcccgccga
     3781 gcgccgcgct gcccgcgacg gcgccgccgc ggccgagcag gccgcggcgt gtcagccgcc
     3841 cgctcatccg cccatcaccg tctcgtcgcc gaactgcggc agcgtcccgc cggcctcctg
     3901 ctccagcacg gcccaaagct cctcgacctt cgctgggttc tgctgggcga ggttgatcgt
     3961 ctccccgggg tcggtcaggg tgtcgaacag gcggatgcgc cgcttctcgc tgtgcgcgat
     4021 cagcacccag cggccgtcgc cgcagatcag atagcgggcg tagcaggcgg tgaagtaggg
     4081 ccgctcctcg tagggccgct tgccgtcgaa caggcgcgag aggtcctcgc cgtccatcag
     4141 ccccggcgcg cggatcccct ggaagccgag cagcgtcgag gcgacgtcgt gggtcgaggc
     4201 gaagtacttg ctgcgctggc ccgccagctt cctgccgccg ggctcgcgga tcatgtaggg
     4261 cacccgatag atcggccagt tcgggtaggc cgcgtgcttg ccgagcaggc cgtactcgcc
     4321 gagcgtcagc ccgtggtcgc tgaggtagac gacggccgtg ttgtccatca gcccgaggtc
     4381 gtccatcttg ttcagcagcc gcccgatcca gcggtcgacg aacgtcagct cggcggcgta
     4441 gagctcgcgc acgcgctcga gggtctcctg gtcgattccg aggtcgaaga cgcggctgta
     4501 aggcgtgtcc cagggctgga tcgggattat cccctcgcgc tcggagccct tgacccgcgc
     4561 gaacttcgtc atgtaggcgc gcggcgggtc gagcgactcg tgcgggtcga aggcgtcgac
     4621 gccgaggaag aacggccgct tgtccttgag ctcgtcgagg cagtccatcc cgctgcggat
     4681 cacccgcgcg gccgagtagt cgctctcgcg gcggcggtag aggttgttcc agccgacgta
     4741 ctcgcgcaac cgctggacct cgatcgagtc cgacagcttc gggaacaggt agcgctcgat
     4801 cgcgctgcgc ggcgccgggc gcttgaacgg ccggttgaag gcgcggtagg aggcctgcga
     4861 gtagtcgggc cgtgccatgt tcagtgtgcg gcggaagttg acgaagcgcg gcccgacgag
     4921 gaacgggttg tcggtcacgt aggcggtctc gacgccggcc tcggacatca cctcggtgaa
     4981 catcggctgg tacgggtaga tgtcgttcca gcctggctcg gcaggcaggt gcggggtcac
     5041 ctgccagttg cggaacggga aggagcgcat gccggtgatc agcgcgcggc gcacggtgcc
     5101 ggtcggcatc gcctccggaa cggcgtggtc gaagagcagc gactccttcg ccagcgccgc
     5161 gaggttgggc gtgtcggcca gctcgtcgcc gtcgtcgtag gcgctgacga agtcgcgccg
     5221 cgtgctgtca gtgacgatca ggacgacgtt gggcgcatcc tcgccgtggc tgatctgcgg
     5281 ctcgaggtcg tcgttgcacg cgccgaggcc gagcgcgagc gcaccggcgc cgccggcctg
     5341 cagcgcacgc cgccgagtca atcgaggttg gagggccatc tgcgggggcg cagactatcc
     5401 agcgcaacag cgggaagcgg cggtcggcgg tcgccggccg tcgaagcgcc gcaggtattg
     5461 tcgccgcccg tgaccgctcc cacattcgat ctccaatccc actccatcca ctccgacggc
     5521 gagctgccga ccgccgaggt cgtcgccaac gcggcggccg ccggcgtcga gctgctggcg
     5581 ctgtcggacc acgacaccgt cgacggcgtc gacgaggcgc tcgccgccgg ccgcgagcac
     5641 ggcgtgcggg tcgtccccgc gacggagatc tcggcggtcg acggcgccta cgaggacatg
     5701 cacgtgctcg gctacggcgt cgaccagagc gacccggtgc tggccgagcg gctgctcgac
     5761 gcccgcggcg accgtgagcg gcgcgccgag gcgatgtccg atcgtctgca ggagctcggc
     5821 ttcgagatcg atccggcgcc ggtcgacgcc cgtcgcgccg ccggcaagcc ggtcggccgg
     5881 cctcacctcg cggccggcgt gctcgcccat ccagcgaacg ccgagcggct cgccgccgaa
     5941 gggcacgcgg acgtctcctc gttcatcccg gcctacctga tccagggcac gcccggctac
     6001 gtggcgcgct cgcacccgac ggtcgccgag gcgatcggct ggatccacga cgccggcggc
     6061 atcgcgatct gggcccatcc cttctgggac gtcgaggacg acggcgaggt gctcgcgctc
     6121 gtcgaccgct tcaaggaggc ggggctggag ggcgtcgagg tcttctacgt cacccacacc
     6181 caggcgcagg tcgagctgct agcggaccgc tgctccgagc tgggcttgct ggcgaccgga
     6241 tcgtcggact accacgggcc cgaccaccgc ctcttcagca gcttccgagc ccaccagacc
     6301 cacgggatcg agccgaacct cggcccgatc ccggagctgg cttcgtgagg cgctaggccg
     6361 cgacgggctg gagcccgaac tccgcgcgga actcgttcgt gtcgtggagg tgcgggaacg
     6421 gctcgtcggg caccgcgacg ccgagtgcgt cggcgagcgg ctcccagccg tccttgccga
     6481 gctcccagac gatcaggcga tcggagtcga cgtacgcctt gatctcctcg atgcggtcgt
     6541 agtacatctg ccgcacccac tccttgtcct cgaagcggcc ctggaagtcg ccctgccaga
     6601 tcagcttctc gatgacctcc cagagcaccg gcgacggcgg ctcgcggctg gcgtcctggg
     6661 tcagctcacc cgccatcgcg gcctgcttga ccgcgaggat cgtgttctgg cagctcttgt
     6721 agaagccgtc gaagtcgcgg tagttgagca gtaccggcac gtccgggaac gcgtcgacga
     6781 tctcgcgcca gcgcgccgcc gccggccagt cgaccgtcgc ctcccagccg tcgaagacct
     6841 cgtgccagtc gacgggctcg ccgtcggcga tcttcttcca gtaggccagg tcgcgctcct
     6901 tgttctcccc catgatcagg tcgatcatgt ggaagcaggg gtcggcgccc agctgctcga
     6961 gcgctccctt gagcgacatc gtgccggtgc gaccgaatcc aactccgatc agcttcatcc
     7021 attcctccta gtgagggtct cgggccagtg gggaagccat ttccccgaat tgcgggggga
     7081 atcctagccc gctggctgag tccccgccgg gccgcggcgg atcgtcggcc cgggaccggc
     7141 acgcggcccg ccgtcctgct gggcctcctg tgcctgctgc atcgctgcca tcgcggccgc
     7201 gcgcgccatc gccggcgagc gccgcatcgt cagcgtgaac gggatcgcga gcaggatcag
     7261 gagcatcgcg gcgcggtagg cccagccgaa ggagttgcgc gccgcgccgg cggtccggcg
     7321 gaagacctgc tcgtcgaagc ccggtcccgg gtcggtgcga tcggtcgagc cgcccgcgaa
     7381 gaccttcatg tcgcgctgca tgagccctgc gaagacggcc ggcggcacct ggtactcctg
     7441 ggcgaggtcg gcgacatcgt cgaccgcgga ctggccgtgc caggtcagcg tcatcgagaa
     7501 ggtcgcgacc agcagcgcga tcccaatcgc tgcgccgagc tggcgcgagg tgttgaggat
     7561 gcccgaggcg aggccgagct cctggccctg gaccgccccc atcgcgccca cgttgatggt
     7621 cgggaagccg gtgcccatgc cgatgccgat cagcaccaga ccggggaaga cgatcaggta
     7681 gtcgacccag ccgtcccaga gcgccggcgt gaacgagacc cagagcaggc cgaccgccat
     7741 cagccccagc gccggagcgg cgaggtcgcg cggcgggcgg ttgtcggcgg ccttgccgac
     7801 gatcggccag acgaggattc ccgtcaccgg aaccggcatc agcgccaacg ctgcctcgaa
     7861 gatcgtgaag ccccagaggt tcacgaacgt cagcgccatc agcagcagcg cgccgagcgc
     7921 gcccgccgcg aacagcagca tcgcgacgtt agcccccacg aactgcttgt tcgactgcag
     7981 gctgcgcgtg atcatcgcgc cgtcgcggcg ctgcgagagc acgaacgcga tcccgagcag
     8041 gatcgaggac tgcagcagga agagcgtcag gccgtcggtc cagccgcggt ggttggcgtc
     8101 gacgaggccg aaggtcaggc agaagaccgc cagcgcgagc agcgtcatgc cgaggtagtc
     8161 gatccgcgtg tccgcgtcga ggtcgtaggt ctcgggccag atccgccgcg ccagcacgat
     8221 gccgagcacg ccgagcggca ggttgacgat gaagatccac tcccagctcg ccagctcgac
     8281 gatcgccccg ccgatcgccg gcccagaggc gctggccgcg agcgcgacga tcgcgatcgt
     8341 cgcaagcgcc ttgccgcgct cctcgatcgg gaagacggcg gcggtgatcg cgaagcccag
     8401 cggcgccagg atgccggcgc cagcgccctg gatgacgcgc gagaggatca gcacgtcggc
     8461 cgacggcgag atcgcgcaga acagcgagcc gagcgtgaag acgcccatcc cgaggatgaa
     8521 gaagcgccgc cggccgaact ggtccgcgag cttgccggcg gcgatcagca gcgccgcgaa
     8581 cgacaggttg taggagttga cgacccaggt gatcgagttg acgtcggtgt cgagctcctg
     8641 ctgcagctcc gggaccgcga cattgacgat cgagacgtcg agcaggctca tcgcccagcc
     8701 gatcatcatc accgcgaaga tcttgtgccg cttcgcgtag agctgctcta cgggagggtt
     8761 ctcgcgccgc ttcgccacga cgcgggagac tagaggtagc cgaggaccgg cgcccccgta
     8821 cccccgccgg acgccggccg gggcgtcgcg atgctcagtt ggggctgccc accgggacga
     8881 cgagcggacg ctcctgctcg gcgaccgcgt cgacgctctc ctcgaggccg ggctcctcgc
     8941 cgtcgccggc gcagaggatc tcgaagcggc cagtgccgat cttctcgtcg gcacgcaccg
     9001 gcacgccgcg g
//
LOCUS       DATABV010000164         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1417732, whole genome shotgun sequence.
ACCESSION   DATABV010000164 DATABV010000000
VERSION     DATABV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417732"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1429
                     /locus_tag="VHF88_07175"
     CDS             287..1429
                     /locus_tag="VHF88_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2161586.1"
                     /translation="MALLCMAAMFAFAACGGDDDESGGGSGGGSAAASGDASKQGAEA
                     SGESLTLPDGLTAGILHVQGNAQSDQLIGYALTDALDALGVEYEERDPQGNPGKTATD
                     LDALIGRDPDVIFGISVEAATIQSQLRRMADRGIPFINVGGTIRDSPLVALSPVPSDY
                     AQAALASQYLLDLLASENEEQPGTPAGKVATFGFDPIYSLLQRRQTFELMAEQNKGVE
                     IVAQHQVNFEDPNADIQGATQDIVQANPDLKAIYTCCDFTLPPIAAGLRQANATDKVK
                     VVSVQLGTPAALDLVENGSVTAVGNAALGASSWMAVDEWARAVAEDREICRDCWHKYE
                     QMFAYDMVAQDNLDVSYELRPDFVVQRLVVDYPAFFTAKWTEEFGL"
     gene            1426..2448
                     /locus_tag="VHF88_07180"
     CDS             1426..2448
                     /locus_tag="VHF88_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2161587.1"
                     /translation="MTTDASMEARHPAGLQDAPPGSAPPWWRTAGPHLRRWGVLGTFA
                     LAFLVFAIAKPDQFLAWDNFSNILSSSAVPMIVACGVTVPLIMSDFDLSIGGVVAFGG
                     SIAVVLQTEQQWYWAGAVLLALAVAIGIGKLHGFLIARLGGSSFVITLALGSVYSGLV
                     LWITDSKTIYSGLEPGFLSLGQDSFLDIRLPIWFALGFALLLFLLTERMVVGRKFYAI
                     GENVTAAYLAGMAVRRLRTYGFVLSALAAAIGGVLLFAEAGSTYSNPGSPLLLAAFSA
                     AFLGAALRANGRFSVLGSAISVVLVQMVTTGLAIVDLPDWTASVFEGSILAIAILIAP
                     STRIWK"
     gene            2445..>2852
                     /locus_tag="VHF88_07185"
     CDS             2445..>2852
                     /locus_tag="VHF88_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2161588.1"
                     /translation="MTAAASERPPASAIAVRGLRKQYPSVVALDDVDADFGRGEVVGI
                     VGKNGAGKSTLIKVLCGAVQPDAGRIELDGEPVQFGHPAQALDHGIVAMHQQLETFAG
                     LTVAENLLLGTHAFPATASASCTGASSGSAVARR"
BASE COUNT          403 a         1008 c          988 g          453 t
ORIGIN      
        1 gtcgccgagg tcgacgcacg cgacggcggc gccctcggcg gcgaagcgcc gcgcggaggc
       61 gagcccgagc cccgacccgg ctccggtcac gaccgccgtc ttgcccgtca gccgtccgtc
      121 cactcgcagc tcctctcaga tcgcggtcgt tccgccaagc attgcgccga cctcttgcaa
      181 tgtctaggcg aatcatgata agagtagtac ttggaagtgc aaccccagga gaggagttca
      241 gatgagtctt cgagccggtg cctggagggg gcgatggaga tttgccatgg cgctgctctg
      301 catggcagcg atgttcgcgt tcgcggcctg cggcggcgac gacgatgagt ccggcggcgg
      361 ctcgggcgga ggcagtgcgg cggccagcgg cgacgcctcg aagcagggcg ccgaagcctc
      421 cggagagtct ctgaccctgc ccgacgggct gaccgcgggc atcctgcacg tccagggcaa
      481 cgcgcagtcc gatcagctga tcggctacgc gctgaccgac gcgctcgacg cgctcggggt
      541 cgagtacgag gagcgggacc cgcagggcaa cccgggcaag accgcgaccg acctcgacgc
      601 gctgatcggc cgcgaccccg acgtcatctt cgggatctcg gtcgaggcgg cgacgatcca
      661 gtcgcagctg cgccggatgg ccgaccgcgg gattccgttc atcaacgtcg gcggcacgat
      721 ccgcgactcc ccgctggtcg cgctgagccc ggtcccgagc gactacgcgc aggcggcgct
      781 cgcgtcgcag tacctgctcg acctgctcgc ctccgagaac gaggagcagc ccgggacgcc
      841 ggcgggcaag gtcgcgacct tcggcttcga cccgatctac tcgctgctcc agcggcggca
      901 gacgttcgag ctgatggccg agcagaacaa gggcgtcgag atcgtcgccc agcaccaggt
      961 caacttcgag gacccgaacg cggacatcca gggcgcgacc caggacatcg tccaggcgaa
     1021 cccggacctc aaggcgatct acacctgctg cgacttcacg ctgccgccga tcgcggcggg
     1081 cctgcggcag gccaacgcga ccgacaaggt caaggtcgtg tcggtccagc tcggcacgcc
     1141 ggccgcgctc gacctcgtcg agaacggctc ggtcaccgcg gtcggcaacg cggcgctcgg
     1201 ggcgagctcc tggatggcgg tcgacgagtg ggcccgcgcg gtcgccgagg accgcgagat
     1261 ctgccgtgac tgctggcaca agtacgagca gatgttcgcc tacgacatgg tggcccagga
     1321 caacctggac gtctcctacg agctgcggcc cgacttcgtg gtccagcggc tggtggtcga
     1381 ctacccggcg ttcttcaccg ccaagtggac ggaggagttc gggctgtgac gaccgacgca
     1441 tccatggagg cccgccaccc ggcgggcctc caggacgctc ctccgggatc ggcgccgccg
     1501 tggtggcgga cggccggccc gcacctgcgg cgctggggcg tgctgggcac cttcgcgctc
     1561 gccttcctcg tgttcgcgat cgccaagccg gaccagttcc tcgcctggga caacttctcg
     1621 aacatcctct ccagctcggc cgtcccgatg atcgtcgcgt gcggcgtgac ggtgccgctg
     1681 atcatgagcg acttcgacct ctcgatcggc ggcgtcgtcg ccttcggggg ctcgatcgcc
     1741 gtcgtgctgc agaccgagca gcagtggtac tgggctggcg cggtcctgct cgcgctcgct
     1801 gtcgcaatcg ggatcggcaa gctgcacggc ttcctgatcg cccgtctcgg cggctcgtcg
     1861 ttcgtgatca cgctcgcact cggctcggtc tactcgggcc tggtgctctg gatcaccgac
     1921 tcaaagacga tctactcggg cctcgagcca ggcttcctgt cgctcggtca ggactccttc
     1981 ctcgacatcc ggctgccgat ctggttcgcg ctcggcttcg cgctgctgct cttcctgctc
     2041 accgagcgga tggtcgtcgg gcgcaagttc tacgcgatcg gcgagaacgt gacagcggcc
     2101 tacctggccg gcatggcggt ccgccggctg cgcacctacg gcttcgtcct gtcggcgctg
     2161 gcggccgcga tcggcggcgt gctgctgttc gccgaggccg gctcgacgta ctccaacccc
     2221 ggctcgccgc tgctgttggc ggcgttctcg gcggcgttcc tgggcgccgc gctgcgcgcc
     2281 aacggccgct tcagcgtgct cggctcggcg atctcggtgg tcctcgtcca gatggtgacc
     2341 accggcctgg cgatcgtcga cctgccggac tggaccgcga gcgtctttga gggctcgatc
     2401 ctggcgatcg cgatcctgat cgctcccagc acgaggatct ggaagtgacg gcggccgcct
     2461 cggagcggcc gccggcgagc gcgatcgccg tccgcggcct gcgcaagcag tacccgtccg
     2521 tggtggcgct ggacgacgtc gacgccgact tcggccgcgg cgaggtcgtc ggcatcgtcg
     2581 gcaagaacgg tgccggcaag agcacgctga tcaaggtcct ctgcggcgcg gtccagcccg
     2641 acgccgggcg gatcgagctc gacggcgagc cggtccagtt cggccacccg gcgcaggcgc
     2701 tcgatcacgg catcgtcgcg atgcaccagc agctcgagac cttcgccggt ctgaccgtcg
     2761 cagagaacct gctgctgggg acgcacgctt tcccggcgac cgcgtcggcc tcgtgcactg
     2821 gcgcgagctc tggaagcgcg gtcgcgcgac gc
//
LOCUS       DATABV010000165         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2702321, whole genome shotgun sequence.
ACCESSION   DATABV010000165 DATABV010000000
VERSION     DATABV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2702321"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /locus_tag="VHF88_07190"
     CDS             <1..1885
                     /locus_tag="VHF88_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX2161589.1"
                     /translation="DYEVIDKEVKIIDEFTGRILEGRRWSEGLHQAVEAKEGVAIQEE
                     NQTVATITYQNYFRRYDKLAGMTGTALTEATEFMKIYDLQVVEIPTNRPMARVDQNDQ
                     IYKTKPGKWNAVAEAIQERHDAGQPVLVGTISVEVSELLSAELRKRGIEHAVLNAKPE
                     YAEREGEIIAEAGRPGAVTIATNMAGRGVDIKLGGNAEHLTELELRGKYEPGTPEYDK
                     AWEEAYPRFEAQVAEDREKVMEAGGLFILGTERHESRRIDNQLRGRSGRQGDPGESRF
                     YLSAEDDLVRLFAGDRIYRILDRLGPTGEDGKEEPIEASMLSKQIEGAQKKVEEQNFL
                     IRKRVLEYDDVMNQQREIIYEYRDRILEGEDMSDTAREQLAEVVERLANQYMEGDFVE
                     NWDLQGLYTQLEQMYPVGLDLSDMDPKQVDRASLIAELREDIVEAYGDREEELGEDLM
                     RALERFMLLQIIDERWREHLHDMDYLREGIHLRGFAQIDPLVAYKNEGFEMFTGLMNA
                     IWEEFGRYIFVVDVQVEGADGDARRTTWGASSNNTSTASFNYAGGTAADQPSALSAAA
                     QAAAATGVAPAGVQNAADLALDDVQAAPRVETRVVADEDRIGRNDPCPCGSGKKYKKC
                     HGA"
     gene            1936..2853
                     /gene="prfB"
                     /locus_tag="VHF88_07195"
                     /pseudo
     CDS             1936..2853
                     /gene="prfB"
                     /locus_tag="VHF88_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT          520 a          955 c         1008 g          370 t
ORIGIN      
        1 cgactacgag gtgatcgaca aggaggtgaa gatcatcgac gagttcaccg gccgcatcct
       61 cgagggccgg cgctggtcgg agggccttca ccaggcggtc gaggcgaagg agggcgtggc
      121 gatccaggag gagaaccaga ccgtcgccac gatcacctac cagaactact tccgccgcta
      181 cgacaagctc gccggcatga ccggcacggc gctcaccgag gcgaccgagt tcatgaagat
      241 ctacgacctg caggtggtcg agatcccgac caaccggccg atggcgcgcg tcgaccagaa
      301 cgaccagatc tacaagacca agccgggcaa gtggaacgcc gtcgccgagg cgatccagga
      361 gcgccacgac gccggccagc cggtgctcgt cggcacgatc tcggtcgagg tgtccgagtt
      421 gctctcggcc gagctgcgca agcgcgggat cgagcacgcc gtgctgaacg cgaagcccga
      481 gtacgccgag cgcgagggcg agatcatcgc cgaggcgggg cggccgggag cggtgacgat
      541 tgcgaccaac atggccggcc gtggcgtcga catcaagctc ggcggcaacg ccgagcactt
      601 gaccgagctc gagctgcgcg gcaagtacga gcccggcacg ccggagtacg acaaggcgtg
      661 ggaggaggcc tacccgcgct tcgaggcgca ggtcgccgag gaccgcgaga aggtgatgga
      721 ggccgggggg ctgttcatcc tcggcaccga gcgtcacgag tcgcgccgga tcgacaacca
      781 gctgcgcggc cgctcaggcc gtcaggggga tccgggcgag tcgcgcttct acctctcggc
      841 tgaggacgac ctcgtccggc tgttcgccgg cgaccgcatc taccgcatcc tcgatcgcct
      901 cggtccgacc ggcgaggatg gcaaggagga gccgatcgag gccagcatgc tctcgaagca
      961 gatcgagggc gcccagaaga aggtcgagga gcagaacttc ctgatccgca agcgcgttct
     1021 cgagtacgac gacgtgatga accagcagcg cgagatcatc tacgagtacc gcgaccgcat
     1081 cctcgagggc gaggacatgt ccgacacggc gcgcgagcag ctcgccgagg tcgtcgagcg
     1141 gctggcgaac cagtacatgg agggcgactt cgtcgagaac tgggacctgc agggcctcta
     1201 cacccagctc gagcagatgt accccgtcgg cctcgacctc tccgacatgg accccaagca
     1261 ggtcgaccgc gcgtccttga tcgccgagct gcgcgaggac atcgtcgagg cctacggcga
     1321 ccgcgaggag gagctcggcg aggatctgat gcgggccctc gaacggttca tgctgctcca
     1381 gatcatcgac gagcgctggc gcgagcacct gcacgacatg gactacctgc gcgagggcat
     1441 ccacctgcgc gggttcgccc agatcgatcc gctcgtggcc tacaagaacg agggcttcga
     1501 gatgttcacc ggcctgatga acgcgatctg ggaggagttc ggccgctaca tcttcgtcgt
     1561 cgacgtccag gtcgagggcg ccgacggcga cgcgcgccgc accacatggg gcgcctcttc
     1621 gaacaacacc tcgacggcgt cgttcaacta cgccggcggc accgccgcgg accagccgag
     1681 cgcgctcagc gccgccgcgc aggccgccgc tgcgaccggc gtggctccgg ccggcgtcca
     1741 gaacgccgcc gatctcgcgc tcgacgacgt ccaggcagcc ccccgcgtgg agacccgtgt
     1801 cgtcgcggac gaggaccgca tcggccgcaa cgacccgtgt ccctgcggct ccggcaagaa
     1861 gtataaaaag tgccatggcg cctagcgcca gtggcctccg cgaccagctg aagctgctcg
     1921 cggactacct ttgaccccgc ttcgctgagc gagcgcgccg acgcgctcga gcgccagatg
     1981 ggcagcgagg ggttctggga cgaccaggcg accgcggcca aggtgtcggc cgagcactcg
     2041 cgcgtccagc gccggctcga cgagttccgc acgctggagt ccgacatcgg cgacctcgag
     2101 gagctcgagg agctggccgg agaggacgag tcgatcgccg cggaggtcgg cgagcagcgg
     2161 gcgtcgatcg aggcgcgcct ggcggcgctc gaggaggcgc ggctgttctc aggcgactac
     2221 gactccggcg acgcggtcgt caccgtcaac gccggcgcgg gcggcaccga ctcgcaggac
     2281 tgggccgaga tgctgctgcg catgttgatg cgctgggccg aggcgcgcga tatgaaggtc
     2341 gagctcaagg aggccagcga gggcgaggag gcgggtctga agtcggcgac cttcaccgcg
     2401 gcgggcgaga acgcctacgg cctcttctct gccgagaagg gcgtccaccg gctggtgcgg
     2461 atctcgccgt tcgactccgc ggcccgccgt cacaccgcgt tcgcgggggt cgaggtcgcc
     2521 ccgctggtcg aggacgccgt cgacgtcgag atcgagcccg acgacctgca gatcgacacc
     2581 tatcgcgctt cgggcgccgg cggccagcac gtcaacaaga ccgactcggc ggtgcggatc
     2641 acccaccggc cgtccggcat cgtcgtccag tgccagaacg agcgctcgca gtcggctaac
     2701 aaggacacgg cgatgcgcat gctgcgcgcg aagctgatcg agcgcgagga gcagcggcgc
     2761 caggaggaga tcgccaagga gaagggcgag gcccaggacg tcggctgggg ctcgcagatc
     2821 cgctcctacg tgctgcaccc ctacacgatg gtc
//
LOCUS       DATABV010000166         7458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1096174, whole genome shotgun sequence.
ACCESSION   DATABV010000166 DATABV010000000
VERSION     DATABV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096174"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..680)
                     /locus_tag="VHF88_07200"
     CDS             complement(186..680)
                     /locus_tag="VHF88_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX2161590.1"
                     /translation="MSLGSSPLPPLAPDDHVRGAGRLVIEYADLECPYCARANLLLAE
                     LPVRRVFRHFPIVSKHPRARVLAQALEAAGIQGRFWEMHGSLLGDQGHLDDPHLWERC
                     TELGVDVARFDSDRRSDAAAERVQRDFRSGVRAGVMTTPTLFVDGVAHPGVPDRDLLA
                     RLGG"
     gene            complement(690..1151)
                     /locus_tag="VHF88_07205"
     CDS             complement(690..1151)
                     /locus_tag="VHF88_07205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX2161591.1"
                     /translation="MIEAGEPAPDFSLSDQDGDTVSLKDLEGQYSVLVFFPAAFSPVC
                     TDQLSVYEEVLPDLRARDAVLYGVSVDGAFALKAFRESLGTTIPLLSDFHPKGEVAKA
                     YGVWVPDRGVATRALVMIGPDLRVVWTHQPPSPLEIPGANLIFDALDGVAA"
     gene            complement(1148..1417)
                     /gene="rpsO"
                     /locus_tag="VHF88_07210"
     CDS             complement(1148..1417)
                     /gene="rpsO"
                     /locus_tag="VHF88_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX2161592.1"
                     /translation="MTLTVDQKQEIVSKHGKDANDTGSAEVQIALLTARVNELTEHLR
                     THKKDHHSRRGLLMLVGKRRRLLDYLQRTDLDRYRALIKELGLRR"
     gene            1604..2065
                     /locus_tag="VHF88_07215"
     CDS             1604..2065
                     /locus_tag="VHF88_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161593.1"
                     /translation="MRAVNRLDVILGQWDDMHGIDGAPAGAHNSLRRAMRRLVPESAA
                     DAIAAIEVGNEDKAVVVLQGHTLYIAKATAGGDVEVRKEPLDAARISVVMVEPRGEAG
                     RVRNWVFERDGGPFLRMRTRRTGRGQNDDAPDNEPFALALCRTVGWDVPAE"
     gene            complement(2337..3233)
                     /locus_tag="VHF88_07220"
     CDS             complement(2337..3233)
                     /locus_tag="VHF88_07220"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX2161594.1"
                     /translation="MKVISLADMPRLEPGAASGRRVAIGTFDGVHVGHREVIRGADTV
                     LTFDPHPVSVIHPEAAPKLLAPFSIKRDLIAGLGVQELVVIPFDREFASRSAEQFVDD
                     VLVGQLGAQSVSVGENFRFGRGAKGTPEFLAADDRFETRVVPLVEAAGETASSSHIRG
                     LVAAGDVRTASEFLGGPFLFEGEVVAGDQRGRELGMPTANLVPDDALVCPSNGVYAAW
                     AHGHPAAVNVGVRPTFDTGRGLLIEAYLIGFDGDLYGETLRIAFVERMRGEKRFDSVD
                     ALVEQMHADVREAERICAATSY"
     gene            complement(3230..4036)
                     /gene="truB"
                     /locus_tag="VHF88_07225"
     CDS             complement(3230..4036)
                     /gene="truB"
                     /locus_tag="VHF88_07225"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX2161595.1"
                     /translation="MSTAASGVILYPKPAGVTSHDCVARVRRTLPRKTKVGHAGTLDP
                     FATGLLLILVGPLTRAQRWLLPLPKTYVAVARLGWRSDTGDRDGELQETGRRPEDPRI
                     ATGDLMQRPPAYSAVRVGGERLYEKARRGEAVEGAPRPVTVYRAERLWHEGDRAAFEI
                     ECSSGTYVRQLVAGLGDAYCEELERTAIGPFRLADADPERIVTPNAALSFLPERPLDA
                     SEATAVANGIRVPAVDGEEGAATRLTHGGEVVAIGERRGHELQPVVVFAR"
     gene            complement(4043..5035)
                     /locus_tag="VHF88_07230"
     CDS             complement(4043..5035)
                     /locus_tag="VHF88_07230"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX2161596.1"
                     /translation="MSGVGTELDQVVEELRGADKLLLTTHENPDGDALGSLLGMHGIL
                     TQLGKDALMYLSPDEFPLPHEYRDMENRDIIGGPPADMDERVAVFLDCGNIDRMPVDF
                     LQRDGIHILNIDHHHDNTRFGTANLVDSKASCTAEIVWRLSKELGVELTTEIARALYV
                     GLITDTGRFMYENTTSEAHQMAAELIEAGVEPHVIYSRIYESLPFERITLLQRGLATI
                     RRFDGGTITMLHLTAEDFEQTGAVENDSEGIIDHARAVEGTAVAVLVRELTGDRAGKR
                     KVSLRATDGRVDVSEIARSFGGGGHRQAAGATTELDLETLTEAIRERVRAQLAA"
     gene            complement(5032..5388)
                     /gene="rbfA"
                     /locus_tag="VHF88_07235"
     CDS             complement(5032..5388)
                     /gene="rbfA"
                     /locus_tag="VHF88_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX2161597.1"
                     /translation="MPADRMRRVNEALREVLSARIAGGLKDPRVGFVTVTAVDTSPDL
                     RHARVYVSVLGDEEERADSLAGLESSRGFLQAGIARELRIKHTPALRFMHDEALERGM
                     RISELLDEVDEEGPRS"
     gene            complement(5412..7214)
                     /gene="infB"
                     /locus_tag="VHF88_07240"
     CDS             complement(5412..7214)
                     /gene="infB"
                     /locus_tag="VHF88_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX2161598.1"
                     /translation="MEPDFSQQEEKPHEEQITDIKSGATVKEVAESLGLSAPDVIKKL
                     MELGEMATLTQTLSDEAIEILADGFEKKVNIVRAADEVTDDVVFEDDDADLVERPPVV
                     TVMGHVDHGKTSLLDAIRESEVAAGEAGGITQHIGAYQVHHGDHTITFLDTPGHAAFT
                     AMRARGASVTDIAVVVVAADDGVMPQTVEAIDHSKAAEVPIVVAVNKIDKDGADPQRV
                     RGELAQHELTPADWGGDTEFVDVSAKARTNLDELLETLTTLSDIQELKANPNTEASGV
                     VIESRLDPGRGPVVSVLISRGTLKVGDAVVAGEHPGRARAMHDYLGERIKAATPGTPV
                     EILGFDGVPEAGEQFRVVANEREARRIAGERANRLKTEALARRAGVKVSLEDVLARAQ
                     KGELNELALIVKADVAGSLEALADEIAKLPQDEVAVNLIHTGVGGINESDVMLASASN
                     AVIIGFNVRPVGDARAVADREGVEIRGYSVIYKVVEELRAAMEGMLKPEDVEETVGTV
                     EIRAIFRASRVGTIAGSMVTDGSVRRGATVRLVRDGTIVYDGRIGSLRRFKEDVREVT
                     TGMECGIVLENFADVKEGDVLEVYETRQVEKTLQ"
BASE COUNT         1047 a         2593 c         2665 g         1153 t
ORIGIN      
        1 cgcactacga cagaaccgct cgcctgcttg gcgagctttc cggtctcgaa gctgatctcg
       61 gcaccgccga cctcaactga gacccgggtc acatcactca tatgttcctt tcaatgccgc
      121 ctccggttgg cggctcgctt catgacttct cgataggagt cccgcgcagc ctagcagcgc
      181 tacggtcatc ccccgaggcg ggcgagcagg tcccggtcgg gcacgcccgg gtgggcgacg
      241 ccgtcgacga acagcgtcgg cgtcgtcatg acgccggccc ggacgcccga gcggaagtcg
      301 cgctgcaccc gctcggcggc ggcgtccgag cgacggtcgg agtcgaaccg cgcgacgtcg
      361 acgcccagct ccgtgcagcg ctcccagagg tgcggatcgt cgaggtggcc ctggtcgccg
      421 agcagcgagc cgtgcatctc ccagaagcgt ccctggatcc ccgccgcctc gagcgcctgg
      481 gccagcacgc gcgcccgcgg atgcttcgag acgatcggga agtggcggaa gacccgccgt
      541 acgggcagct cggccagcag caggttcgcc cgggcgcagt aggggcactc gaggtcggcg
      601 tactcgatca ccagccgtcc ggcaccgcgg acgtggtcgt ccggcgcgag cggcggaagc
      661 gggctcgagc cgaggctcac gcccgggcgt caggcggcga caccgtcaag cgcgtcgaag
      721 atcaggttcg cgcccgggat ctcgagcggg ctcggcggct ggtgggtcca gacgacgcgc
      781 agatcgggac cgatcatgac caacgcccgg gtcgcgacgc cgcggtccgg cacccagaca
      841 ccgtaggcct tcgccacctc gcccttcgga tgaaagtccg acaacagcgg aatcgtggtc
      901 ccaaggctct cgcggaacgc cttcagtgcg aacgccccgt cgaccgagac gccatagagc
      961 acggcgtcgc gcgcgcgtag gtccgggagc acctcctcgt agacgctcag ctggtcggtg
     1021 cagaccgggc tgaacgcagc cggaaagaac accaggacgc tgtactggcc ctccaggtcc
     1081 ttcagcgaga cggtgtcgcc gtcctggtcg gacagcgaga agtcgggggc gggctccccg
     1141 gcctcgatca tcggcggagg ccgagctcct tgatcagcgc gcgatagcgg tcgaggtcgg
     1201 tgcgctgcag gtagtcgagc aggcgacggc gcttgccgac cagcatcagc agcccgcggc
     1261 ggctgtggtg gtccttcttg tgcgtgcgca ggtgctcggt cagctcgttg acgcgcgcgg
     1321 tcagcagcgc gatctggacc tcggcggagc cggtgtcgtt cgcgtccttg ccgtgcttgg
     1381 agacgatctc ctgcttctgg tcgacggtca gcgtcatgcg gccggggaag atagcagccg
     1441 gggcgacgcg gtctcggcgc tggtggcagg gtctcgggct agggctcggc gttggtggcc
     1501 gcggggcccg tctccggtgg cggtttccgt gctcggctcc gcggcgactt cggtaagcgt
     1561 gtcgcgtgcg gttcacgtct ggttgacgga ttaccgtccg cgtatgaggg cggtgaaccg
     1621 gctcgacgtg atccttggcc agtgggacga catgcacggc atcgacggcg cacctgccgg
     1681 tgcccacaac tcccttaggc gcgctatgcg gaggctcgtg cccgagtccg cggcggatgc
     1741 gattgctgcc atcgaggtcg gcaacgagga caaggcggtc gtggtccttc aaggtcacac
     1801 cctctacatc gctaaggcaa ctgccggcgg tgatgtagag gtccgcaagg agccactaga
     1861 cgcggctcgt ataagtgtgg tcatggttga gccgcgcggc gaggctggac gggttcgcaa
     1921 ttgggtcttc gagcgggatg gcggcccctt tctgcgtatg cgcactcgac gaaccggcag
     1981 aggccaaaat gacgacgcac ctgacaacga gccgttcgcg ctcgcgctct gccgcaccgt
     2041 tggatgggac gtgccggccg agtagcgcga cccgcgctgc gtcttcgtaa cgacttcctc
     2101 ctggatggtt tgcagaaggc ggctgtagac gctgcttccg cgggtggcga cggtggcggg
     2161 attgcgcgcg caggcgcagc aggcgcgcag cccctgccaa ctgtccacgt gagcgcccta
     2221 agcggcaagg agcgctcggc acggggcgaa gtcgccgcgg agccgagcac ggaaaccgcc
     2281 accggagacg ggccccgcgg ccaccaacgc ccagccctgg gtgcttagga ccccgcctag
     2341 tagctggtag cggcgcagat tcgctctgcc tcgcggacgt ccgcgtgcat ctgctcgacg
     2401 agggcgtcga ccgagtcgaa gcgcttctcg ccgcgcatgc gctcgacgaa ggcgattcgc
     2461 agggtctcgc cgtagaggtc gccgtcgaag ccgatcaggt aggcctcgat cagcaggccg
     2521 cggccggtgt cgaaggtcgg ccgcacgccg acgttgacgg cggccgggtg gccgtgcgcc
     2581 caggcggcgt agacgccgtt gctgggacag acgagcgcgt cgtcggggac gaggttggcg
     2641 gtcggcatcc cgagctcgcg gccgcgctgg tcgccggcga cgacctcgcc ctcgaagagg
     2701 aacgggcccc cgaggaactc ggaggcggtg cgtacgtcgc cggcggcgac gagcccacgg
     2761 atgtgactcg aggaggcggt ctcgccggcg gcctcgacga gcggcacgac gcgcgtctcg
     2821 aagcggtcgt cggcggcgag gaactcgggc gtgcccttcg cgccgcgccc gaagcggaag
     2881 ttctcgccga ccgacacgct ctgcgcgccg agctggccga ccagcacgtc gtcgacgaac
     2941 tgctcggcgc tgcgcgacgc gaactccctg tcgaagggga tcacgaccag ctcctggacg
     3001 cctaggcccg cgatcaggtc gcgcttgatc gagaacggcg cgagcagctt aggagccgcc
     3061 tcggggtgga tcaccgagac ggggtgcggg tcgaaggtaa gcaccgtgtc ggcgccgcgg
     3121 atcacctcgc gatggccgac gtggaccccg tcgaaggtgc cgatcgcgac ccggcgccca
     3181 ctcgccgcgc ccggctcgag tcgcggcatg tccgccagcg agatgacctt catcgcgcga
     3241 acactacgac gggttgcagc tcgtggccgc gccgctcgcc gatcgcgacg acctcgccgc
     3301 catgggtcag gcgcgtggcc gccccctcct ccccatccac cgccggcacg cggatgccgt
     3361 tcgcgacggc ggtggcctcg ctcgcgtcga gtggccgctc gggcaggaag gagagcgccg
     3421 cgttcggcgt gacgatccgc tcgggatcgg cgtcggcgag gcggaagggc ccgatcgcgg
     3481 tgcgctcgag ctcctcgcag taggcgtcgc cgaggccggc gacgagctgc cggacgtagg
     3541 tgcccgacga gcactcgatc tcgaaggcgg cgcggtcacc ctcgtgccag agacgctccg
     3601 cgcggtagac ggtgaccgga cgcggcgcgc cctcgaccgc ctcgccacgg cgcgccttct
     3661 cgtagagccg ctcgccgccg acgcgcacgg cgctgtaggc cggcgggcgc tgcatcaggt
     3721 cgccggtcgc gatgcgcgga tcctcgggcc gcctgccggt ctcctgcagc tcgccgtcgc
     3781 ggtcgccggt gtccgaccgc caaccgagcc gggcgacggc gacgtaggtc ttcggcagcg
     3841 gcagcagcca gcgctgcgca cgcgtcagcg ggccgacgag gatcaacagc aggccagtgg
     3901 cgaacgggtc gagcgtgccg gcgtggccta ccttcgtctt gcggggcagc gtccggcgga
     3961 cgcgcgcgac gcagtcgtgc gaggtgaccc cggccggctt gggatagagg atgacgcctg
     4021 acgctgcggt gctcaccggc cgtcaggcgg cgagctgagc gcgaacccgc tcgcggatcg
     4081 cctcggtgag cgtctcgagg tcgagctcgg tcgtggcgcc cgccgcctgg cggtgaccgc
     4141 cgccgccgaa gctgcgcgcg atctcggaga cgtcgacgcg gccgtcggtc gcacgcagcg
     4201 agaccttgcg cttgccggcg cggtcgccgg tcaactcacg gacgagcacc gcgacggcgg
     4261 tcccctcgac cgcgcgggcg tggtcgatga tcccctcgga gtcgttctcg acggcccccg
     4321 tctgctcgaa gtcctcggcg gtcaggtgga gcatcgtgat cgtcccgccg tcgaagcggc
     4381 ggatcgtcgc gagcccgcgc tgcagcaggg tgatccgctc gaacggcagc gactcgtaga
     4441 tccggctgta gatgacgtgc ggctcgaccc cggcctcgat cagctcggcc gccatctgat
     4501 gcgcttccga cgtcgtgttc tcgtacatga agcgaccggt atcggtgatc aggcctacgt
     4561 agagggcgcg tgcgatctcg gtcgtcagct cgacgccgag ctccttcgag aggcgccaga
     4621 cgatctccgc ggtgcacgac gccttcgaat cgacgaggtt cgccgtgccg aagcgcgtgt
     4681 tgtcgtgatg gtggtcgatg ttgaggatgt ggatgccgtc gcgctggagg aagtcgaccg
     4741 gcatccggtc gatgttgccg cagtcgagga agacggccac gcgctcgtcc atgtcggccg
     4801 gcgggccgcc gatgatgtcg cggttctcca tgtcgcggta ctcgtgcggc aacggaaact
     4861 cgtcgggcga gaggtacatc agcgcgtcct tgccgagctg ggtcaggatc ccgtgcatgc
     4921 cgagcagcga gccgagcgcg tcgccgtcgg ggttctcgtg ggtggtgagc agcagcttgt
     4981 cggcgccgcg cagctcctcg acgacctggt cgagctcggt gccgaccccg ctcacgatcg
     5041 tggcccttct tcgtccacct cgtcgagcag ctcggagatc ctcatcccgc gctcgagcgc
     5101 ctcgtcgtgc atgaagcgca gcgccggtgt gtgcttgatc cgcagctcgc gcgcgatgcc
     5161 ggcctgcagg aagccgcgcg aggactcgag cccggccagg ctgtcggcac gctcctcctc
     5221 gtcgccgagc acgctcacgt agacacgtgc gtggcggagg tccgggctgg tgtcgacggc
     5281 tgtcacggtg acgaatccaa cgcggggatc tttcaagccc cctgcgatcc gggcggaaag
     5341 gacctccctc aaggcctcgt tcacgcgccg catgcggtcg gcgggcatcc tactcgccct
     5401 cgcccgaatg cctactgaag ggtcttctcg acctgccgcg tctcgtagac ctcgagcacg
     5461 tcgccctcct tgacgtcggc gaagttctcg agcacgatgc cgcactccat accggtggtg
     5521 acctcacgga cgtcctcctt gaagcggcgc agggagccga tccgaccgtc gtagacgatc
     5581 gttccgtcgc ggacgagcct cacggtagcg ccccgccgca cggagccgtc ggtgaccatc
     5641 gagccggcga tcgtgccaac gcgcgacgcg cggaagatcg ctcggatctc gaccgtgccg
     5701 actgtctcct cgacgtcctc gggcttgagc atgccctcca tcgctgcgcg cagctcctcg
     5761 acgaccttgt agatgaccga gtagccgcgg atctcgacgc cctcgcgatc ggctaccgcg
     5821 cgcgcatcgc cgaccggccg gacgttgaag ccgatgatca cggcgttcga ggccgaggcg
     5881 agcatcacgt ccgactcgtt gatgccgccg acgccggtgt ggatcaggtt gaccgccacc
     5941 tcgtcctgcg gcagcttggc gatctcgtcg gcgagcgcct cgagcgagcc ggcgacgtca
     6001 gccttgacga tcagcgcgag ctcgttgagc tcgcccttct gggcacgcgc gaggacgtcc
     6061 tccagcgaca ccttcacgcc ggcgcgccgc gccagcgcct ccgtcttaag ccggttcgcg
     6121 cgctcgccgg cgatgcgccg cgcctcgcgc tcgttcgcca cgacgcggaa ctgctcaccg
     6181 gcctcgggga cgccgtcgaa gccgaggatc tcgaccgggg tgcccggcgt cgcggcctta
     6241 atccgctcgc cgaggtagtc gtgcatcgca cgcgcgcggc cggggtgctc gccggcgacg
     6301 accgcgtcgc cgaccttcag cgtcccgcgc gagatcagca ccgacaccac gggcccgcgg
     6361 ccagggtcga ggcgggactc gatcaccacg ccagaggcct cggtgttcgg gttggccttc
     6421 agctcctgga tgtcggacag cgtcgtcagc gtctcgagca gctcgtcgag attcgtgcgc
     6481 gccttggccg acacgtcgac gaactcggtg tcgccgcccc agtccgcggg cgttagctcg
     6541 tgctgggcga gctcgccgcg gacgcgctgc gggtcagccc cgtccttgtc gatcttgttg
     6601 actgcgacga cgatcggcac ctcagcggcc ttcgagtggt cgatcgcctc gacggtctgc
     6661 ggcatcacgc cgtcgtcggc ggcgacgacg acgaccgcga tgtcggtgac gctggcgccg
     6721 cgggcgcgca tcgcggtgaa ggcggcatgg ccgggcgtat cgaggaaggt gatcgtgtgg
     6781 tcgccgtggt ggacctggta ggcgccgatg tgctgggtga tgccgccggc ctcgcccgcg
     6841 gctacctccg actcgcggat cgcgtcgagc agcgacgtct tgccgtggtc gacgtggccc
     6901 atcaccgtca cgaccggcgg gcgctcgacg aggtcggcat cgtcgtcctc gaagacgacg
     6961 tcgtcggtga cctcgtcggc ggcgcgaacg atgttgacct tcttctcgaa gccgtcggcg
     7021 aggatctcga tcgcctcgtc ggagagcgtc tgggtcagcg tcgccatctc gccgagctcc
     7081 atcagcttct tgatcacgtc cggcgccgag aggccgagcg actccgctac ctccttgacg
     7141 gtcgcgccag acttgatgtc ggtgatctgc tcctcatgcg gcttctcctc ctgctggctg
     7201 aagtccggct ccacccaggg ggtccgcctg cggcggccgc ggcggcgcgg cggcgtctgc
     7261 tgctgcggcg gtggacctgg cgcccggcgc gaggcctgcg agtcgatcac cacgcggcgg
     7321 cgacgcgccg atgaggtcgg ggggctcgcg ccccccgcgc cccccgcgga accctggccg
     7381 gccttggaag ggctcgcgcc ccccgggccc cccgcggaac cctggccggc ctgggaaggg
     7441 ctccggccgc gcccgccg
//
LOCUS       DATABV010000167         4831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1900469, whole genome shotgun sequence.
ACCESSION   DATABV010000167 DATABV010000000
VERSION     DATABV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900469"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VHF88_07245"
     CDS             <1..826
                     /locus_tag="VHF88_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2161599.1"
                     /translation="LERALDMVGLKLEHVRLLVCTHAHSDHYGLAAPICEAAGCELWM
                     HPNHGHMTAAAQDPAAAFERRIEVARQSGVPMAALERYREENKDRDFGIAGVKLPDRD
                     LVPGVEVDTDLGCWQVVETPGHAPSHVVLHQPERGLLISGDHVLGRPSLYFDFGYTPD
                     PVGEYLTSLERVRALDVDLILAGHGRPARDLEGHIDSHLDLVDEALARVHQALSGEPR
                     TAFEIAGAGLGLERVPRAAMNWALSLTLSYIRRLEVTGQVERVDGSDPERWRLVGS"
     gene            complement(828..1724)
                     /gene="metF"
                     /locus_tag="VHF88_07250"
     CDS             complement(828..1724)
                     /gene="metF"
                     /locus_tag="VHF88_07250"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX2161600.1"
                     /translation="MRIDAILAERRPVFSFEFFPPKTDEGLVNLRETLAVLSDDEPDF
                     VSVTYGALGSTRDTTIDIVKWIKQDLGIEAMAHFTCVGATVEQLRATLDEIEAAGVDN
                     VLALRGDPPAGQQEWVQTEGGLRYSTELIELLDESYGFAVGAAAFPEVHPQAESAESD
                     ISFLKAKQDAGASFLITQLFYDNEFYFDFVERARDAGVTVPIIPGIMPVINLRNIKRI
                     TELCKSEIPEPFERQLEAREDDPQALQDLGVAYATLQCVDLLARGAPGIHFYTLNRSP
                     ATRAILAALRAAEPWTRAAQPV"
     gene            complement(1734..2036)
                     /locus_tag="VHF88_07255"
     CDS             complement(1734..2036)
                     /locus_tag="VHF88_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161601.1"
                     /translation="MAEVWRVPLPAGAKPPLRVFVNAVPQQEGRDYELGDGELLFSRS
                     LVKEKVGFWRWTAMFLALFGSYGRNDSVDVEYTVDGRKVVATNLEFIPPPGRSIHG"
     gene            complement(2070..2411)
                     /locus_tag="VHF88_07260"
     CDS             complement(2070..2411)
                     /locus_tag="VHF88_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161602.1"
                     /translation="MSGYRVTARKGPKVERETYADLNQALAAIERHVGEIERTAHARA
                     TGGRLIRRFEPVQQVVGRVELRGPDGARGGVDVRGDSSSEAYTGRFRRTLVESLAGES
                     PTDALRRALSA"
     gene            2443..3288
                     /locus_tag="VHF88_07265"
     CDS             2443..3288
                     /locus_tag="VHF88_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2161603.1"
                     /translation="MSEAETPAPSTPPAGPLSPRDAIEGVAVHAPSRGNRKLEALLEA
                     ANGDDRVKGWWHMQQVFADRLGMSDHSWVHIQVVLNVALRLFRLLAKRGVEPAMVTDH
                     LMRERDAEVVIAAACLFHDTGMSIHRADHESYSLFLAARALPELLADAYSEPERTVVA
                     AEALHAIIGHRRRGDPITIEAGIVRVADALDLAQGRSRVPFENRRPNIHSLSAAAIDS
                     VEIKAGEERAVTIEIAMNNSAGIFQVDELLATKLRGSGLEQHVEVVARIDAEHEKRLV
                     PVFRI"
     gene            3376..4542
                     /locus_tag="VHF88_07270"
     CDS             3376..4542
                     /locus_tag="VHF88_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HEX2161604.1"
                     /translation="MPVKRRGVSTLSLGLLTVAVAIAPASAAPDSPQVGKRQWIDCSS
                     GHMRGVAPYSVWPDLWRAQREGRSGDVALLERIAHVPQAKWLAGKSVRQDPFRIVRNY
                     VANTMATQWGGPDCQTRYSGNAGADDPYVGDYPVFAIRQLEHEACHRDYDGGGAWNRP
                     RNGSYLPWIEKFIAAIGPIAHEATVILEPDGLPVIGKCLSRRAAKQRLALMRAVTRRL
                     GSLPNLATYIDIGSSRWLDEKRAIKLLRRAGVRHVRGFALNTTHFYYTHEQVEYGDKI
                     ARRLGGKRYVVNTAENGRGGLRIKNPNARNVKNRFCNPKNAGLGPLPTTRTASRFADA
                     YLWISRPGLSSNGHSGMRQCSRGPGGNVFWLPKAEQEAKLAVFDGQPPWPPAPL"
     gene            complement(4539..>4831)
                     /locus_tag="VHF88_07275"
     CDS             complement(4539..>4831)
                     /locus_tag="VHF88_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX2161605.1"
                     /translation="DNPKVEIAVELEKHALDDEYFTDRKLFPNVDFYSGLIYEAMNIP
                     TDMFTVMFAIPRTAGWLAQWKEMLEDDETKIARPRQIYTGPRQADYVPLDQR"
BASE COUNT          710 a         1767 c         1662 g          692 t
ORIGIN      
        1 cctcgagcgc gcgctggaca tggtcggcct gaagctcgag cacgtgcgcc tgctcgtgtg
       61 cacccacgcc cactccgacc actacgggct ggcggcgccg atctgcgagg cggccggctg
      121 cgagctctgg atgcacccga accacggcca catgacggcc gccgcgcagg atccggcggc
      181 ggcgttcgag cgccggatcg aggtcgcgcg ccagagcggc gtgccgatgg cggcgctcga
      241 gcgctaccgc gaggagaaca aggaccgcga cttcggcatc gccggcgtca agctgcccga
      301 ccgcgacctc gtgccgggcg tcgaggtcga caccgacctc ggctgctggc aggtcgtgga
      361 gacgcccggc cacgcaccct cgcacgtcgt cctgcaccag cccgagcgcg ggctgctgat
      421 ctccggcgac cacgtgctcg gccgcccctc gctctacttc gacttcggct acacgcccga
      481 cccggtcggc gagtacctga ccagcctcga aagggtgcgc gcgctcgacg tcgacctgat
      541 cctcgccggc cacggccgtc cggcgcgcga cctcgagggg cacatcgact cgcatctcga
      601 cctcgtcgac gaggccttgg cgcgcgtgca ccaggcgctc tccggcgagc cgcgcacggc
      661 cttcgagatc gccggggcgg gtctgggcct ggagcgggtg ccgcgcgccg ccatgaactg
      721 ggcgctgtcg ctgacgctct cctacatccg gcggctggag gtcacgggcc aggtcgagcg
      781 ggtcgacggc tctgacccgg agcgctggcg actggtcggc tcctaggtca caccggctgc
      841 gcggcgcgcg tccagggctc cgcggcgcgc agcgcggcga gaatcgcccg cgtcgccggc
      901 gacctgttca gcgtgtagaa gtggatcccg ggcgccccgc gcgccagcag gtctacgcac
      961 tgcaacgtgg cgtacgccac accgaggtcc tgcagcgcct gggggtcgtc ctcgcgcgcc
     1021 tcgagctggc gctcgaacgg ctcggggatc tcggacttgc agagctcggt gatccgcttg
     1081 atgttgcgga ggttgatcac cggcatgatc cccgggatga tcgggacggt cacgccggcg
     1141 tcgcgcgccc gctcgacgaa gtcgaagtag aactcgttgt cgtagaagag ctgggtgatc
     1201 aggaagctcg ccccggcgtc ctgcttcgcc ttgaggaagc tgatgtccga ctccgcgctc
     1261 tcggcctggg ggtggacctc cgggaacgcc gcggcgccga ccgcgaagcc gtagctctcg
     1321 tcgagcagct cgatcagctc ggtcgagtag cgcagcccgc cctcggtctg aacccactcc
     1381 tgctggcccg cgggcggatc gccgcgcagc gcgaggacgt tgtcgacgcc ggccgcctca
     1441 atctcgtcga gcgtcgcgcg cagctgctcg accgtcgccc cgacgcaggt gaagtgcgcc
     1501 atcgcctcga tgccgaggtc ctgcttgatc cacttgacga tgtcgatcgt cgtgtcgcgc
     1561 gtcgaaccga gcgcaccgta ggtcaccgag acgaagtccg gctcgtcgtc gctcagcacg
     1621 gcgagcgtct cgcgcaggtt gaccagcccc tcgtcggtct tcggcgggaa gaactcgaac
     1681 gagaagaccg ggcgccgctc ggccaggatg gcgtcgattc gcatgcgaaa aggctacccg
     1741 tggatcgagc ggccgggcgg cgggatgaac tcgaggttgg tggcgaccac cttgcgcccg
     1801 tcgaccgtgt actcgacgtc gaccgagtcg ttgcgcccgt agctgccgaa cagcgcgagg
     1861 aacatcgcgg tccagcgcca gaagccgacc ttctccttga ccagtgagcg cgagaacagc
     1921 agctcgccgt cgccgagctc gtagtcgcgc ccctcctgct gcggcacggc gttgacgaac
     1981 acgcgcagcg gcggcttggc gcccgcgggc aggggaactc gccagacctc ggccatcgcg
     2041 cggttatagc ggtcggccgg gtgggcgcgc taggcgctca acgcgcggcg cagcgcatcg
     2101 gtcggcgact cgcctgccag cgactcgacc agggtgcgcc ggaagcggcc ggtgtaggcc
     2161 tcgctggagg agtcgccgcg gacgtctacg ccgccgcgcg cgccgtccgg tccgcgcagc
     2221 tcgacccgcc cgacgacctg ctggaccggc tcgaagcggc ggatcagccg cccgccggtc
     2281 gcgcgcgcgt gcgccgtccg ttcgatctcg ccgacgtggc gctcgatcgc ggcaagcgcc
     2341 tggttcaggt ccgcataggt ctcgcgctcg accttcggcc ccttgcgcgc cgtgacccgg
     2401 tagccgctca cccgtcgcag cgtagagcta tgttcggcgg cgatgagcga ggccgagaca
     2461 ccggcgccct ccacaccgcc cgccgggccg ctgagcccgc gcgacgcgat cgagggcgtg
     2521 gccgtgcacg cgccgagccg cggcaaccgc aagctcgagg cgctgctcga ggcggccaac
     2581 ggcgacgatc gcgtcaaggg ctggtggcac atgcagcagg tgttcgccga ccggctcggg
     2641 atgtccgacc actcctgggt ccacatccag gttgtgctca acgtcgccct gcggctgttc
     2701 cggctgctcg ccaagcgcgg cgtcgagccg gcgatggtca ccgaccacct gatgcgcgag
     2761 cgcgacgccg aggtcgtgat cgccgccgcc tgcctgttcc acgacaccgg catgtcgatc
     2821 caccgcgccg accacgagtc ctacagcctc ttcctcgccg cccgggcgct gcccgagctg
     2881 ctcgccgacg cctactccga gcccgagcgg acggtcgtcg ccgccgaggc gctgcacgcg
     2941 atcatcggcc accgccgccg cggcgacccg atcacgatcg aggcggggat cgtccgcgtc
     3001 gccgacgcgc tcgacctcgc ccagggccgc tcgcgcgtcc cgttcgagaa ccgccggccg
     3061 aacatccact cgctgtcggc cgcggcgatc gacagcgtcg aaatcaaggc cggcgaggag
     3121 cgggccgtca cgatcgagat cgcgatgaac aactcggcgg gcatcttcca ggtcgacgag
     3181 ctgctggcca ccaagctgcg cggctccggc ctcgagcagc acgtcgaggt cgtcgcgcga
     3241 atcgacgccg agcacgagaa gcggctcgtc ccggtcttcc gcatctgatt cgggatcgtt
     3301 cagcctcggg cgggcacagc tctgcaatct ctgcacggga cgtcagcgcg ccggacgccg
     3361 ggtacgtagg tgttcatgcc ggtcaagcgc agaggcgtct cgacgctctc cctggggctg
     3421 ctcacggtcg ccgtggcgat cgcgcccgct agcgccgcgc cagacagccc gcaggtcggc
     3481 aagcggcagt ggatcgactg ctcgagcggg cacatgcgcg gcgtcgcgcc gtactccgtc
     3541 tggccggacc tctggcgggc gcagcgcgag ggccgcagcg gcgacgtggc gctgctcgag
     3601 cggattgccc acgtcccgca ggcgaagtgg ctcgcgggca agagcgtgcg ccaggacccg
     3661 ttccggatcg tgcgcaacta cgtcgcgaac acgatggcga cccagtgggg cggccccgac
     3721 tgccaaacgc gctactcggg caacgccggc gccgacgacc cctacgtcgg cgactatccg
     3781 gtcttcgcga tccgccagct cgagcacgag gcctgccacc gcgactacga cggcggcggg
     3841 gcgtggaacc ggccccgcaa cggcagctac ctgccttgga tcgagaagtt catcgcggcg
     3901 atcggcccga tcgcccacga ggcgaccgtg atcctcgagc ccgacggcct gccggtgatc
     3961 ggcaagtgcc tgagccgccg cgcggccaag cagcggctcg ccctgatgcg cgccgttacc
     4021 cggcggctcg gcagcctccc gaacctcgcg acttacatcg acatcggctc ctcgcgctgg
     4081 ctcgacgaga agcgcgcgat caagctgctg cgacgggcgg gcgttcgcca cgtgcgcggc
     4141 ttcgcgctca acacgaccca cttctactac acccacgagc aggtcgagta cggcgacaag
     4201 atcgcgcgcc ggctcggcgg caagcgctac gtcgtcaaca cagccgagaa cggccgcggc
     4261 ggcctgagga tcaagaaccc gaacgcgcgc aacgtcaaga accggttctg caacccgaag
     4321 aacgccggtc tcgggccgct gccgacgacc cgcacggcct cccgcttcgc cgacgcctac
     4381 ctctggatct cacgccccgg cctctcgtcc aacggccaca gcgggatgcg ccagtgcagc
     4441 cgtggccctg gcggcaacgt cttctggctc cccaaggccg agcaagaggc gaagctcgcc
     4501 gtgttcgacg gccagccgcc ctggccgccg gcgcccctct agcgctggtc gagcggcacg
     4561 tagtcggcct ggcgcgggcc ggtgtagatc tgccgcggac gcgcgatctt cgtctcgtcg
     4621 tcctccagca tctccttcca ctgggcgagc cagcccgcgg tgcgggggat cgcgaacatc
     4681 accgtgaaca tgtcggtcgg gatgttcatc gcctcgtaga tcaggcccga gtagaagtcg
     4741 acgttcggga acagcttgcg gtcggtgaag tactcgtcgt cgagcgcgtg cttctcgagc
     4801 tcgacggcga tctcgacctt cgggttgtcg a
//
LOCUS       DATABV010000168        14506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1096806, whole genome shotgun sequence.
ACCESSION   DATABV010000168 DATABV010000000
VERSION     DATABV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14506
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096806"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VHF88_07280"
     CDS             complement(<1..747)
                     /locus_tag="VHF88_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2161606.1"
                     /translation="MRQKSPETYEEKLAQLEELREQAIHTNPQAEEKQHARGKLTARE
                     RVEKLLDPGSFQELDTFVRHRTFDFDMQKNRPWGDAVVTGHGTIDGRPVCVFSQDFTV
                     FGGSLGEVMAEKMCKIMDLAAKIGCPVIGINDSGGARIQEGVVSLGAYGDVFVRNVQC
                     SGVIPQISLIMGPCAGGAVYSPAMTDFIFMVKETSHMFITGPDVIKTVTGEEVEFEEL
                     GGAMTHNTKSGVAHFASEDEEACLEDARYLM"
     gene            complement(798..1451)
                     /locus_tag="VHF88_07285"
     CDS             complement(798..1451)
                     /locus_tag="VHF88_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161607.1"
                     /translation="MADLTAQRTLVKSPPELWSELSEVESLAKHLGELGEIRITKVEP
                     ESTVAWEGEEVSGTVALEQSGWGTKVTFTATVPEPGPADLPAPAEPEPAAVAVEHEPV
                     VVEPVAVEPEPVAVEPAPVEREPEPKRQGFLARWLFRQRTPRGLAVDPTPVAIEQVPA
                     LEPTPAPEPVVDPEPQPQPEPEPEPLVANAEAERIQRILDGTLEALGQAHHRPFSRG"
     gene            complement(1513..2619)
                     /locus_tag="VHF88_07290"
     CDS             complement(1513..2619)
                     /locus_tag="VHF88_07290"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2161608.1"
                     /translation="MSGVAVTPRDRVEAAICVALAKLPLGAQRLLGGTPIRIDGLELH
                     PTSQLLVKLEQLADVPPVESLPVADARAQVGRQAALLTGPKLPLARVEDHSIPGPAGE
                     IRARLYSPFPPPAEGFDSPGDANPSSAAPGGLLVYYHGGGHVIGTLDTHDSACRFLAM
                     HASAGVLSIDYRLAPEHPYPAAVDDSIAALAWASENAKRLGFDPARIAVGGDSAGGNL
                     AAVVALAAKAGEAPLPAFQLLIYPVCDYVEKRRSYELFRDGFLLTAAEMDWFRDHYLP
                     DRDAAHEWRASPLQAPDLSGLPPAYVLTAGFDPLRDEAEEYARALIAAGVPTALRRHD
                     GLLHSFVNQTTLHRGAHDAMLEAAGALRLALAPH"
     gene            complement(2624..4243)
                     /locus_tag="VHF88_07295"
     CDS             complement(2624..4243)
                     /locus_tag="VHF88_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2161609.1"
                     /translation="MATTPDARPSIAPIVGPDDPRRFTDSGIEIKPLYTEDDLPPDLP
                     EKLGKPGDYPFTRGVHERMYRDRQWTMRQYAGYATAQETNKRFHYLIDHGSTGLSMAF
                     DLPTQLGRDSDDPLCLGEVGRTGVAIDTIDDMRRVFDGIPLDKVSTSMTINAPAACLL
                     LLYELVGEEQGVESHVLRGTTQNDILKEYIARGNFIYPPQPAMRLTTDLFAYCRERVP
                     KWNTISISGYHMREKGCSAVQEVGFTLANAIAYVQAAIDAGLDVDEFGPRLAFFFNGH
                     NNVFQEVAKFRAARRMWAQIMRERFGATNPKSQTIRFHTQTGGVTLAAQQPENNIVRV
                     ALQGFAAVCGGTQSLHTNGYDEALALPTEKAARIALRTQQIIAAESGATDTVDPFAGS
                     YFIEALTDEIEAGAQALIDKVDDMGGSVQCIPFIRAEVEQSAWGYEERYRIKQDIVVG
                     VNEYVTNDVDEVETLRVDPQSEHEQVARLKEFKANRDQAAVDSALAELRATAQGTGNL
                     LPPIRAALKARASIGEVCNVMRDEFGEYSEA"
     gene            complement(4259..4915)
                     /locus_tag="VHF88_07300"
     CDS             complement(4259..4915)
                     /locus_tag="VHF88_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2161610.1"
                     /translation="MRGSDAERLIEEHGASMLATARRWSATDEDAEDAFQRAVERVLR
                     RHPTGEAQELRGWLRTTVKNEALAIARHRRRIVTGGTAESLTGTRMFSTHETPNTDAR
                     AEQLERLRLGSQALRRLKPQEIRALQLQAEGYSYKEIAEITGWSATKVNRCLAEGRHA
                     FRQAIEGIESGSECGRLATTLDAFAAGDASSDEVAPVRRHLATCLACRARLRQLRAAA
                     "
     gene            5228..6655
                     /locus_tag="VHF88_07305"
     CDS             5228..6655
                     /locus_tag="VHF88_07305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2161611.1"
                     /translation="MRAGSVPLLKGVLRFEALRRGGRVLTLAALDVAGMFLAIWTALA
                     IKAAVRAPGILGSTFEQAQEYAPLACLVMLLLFARSGLYRDRAQRPGFTNVVSGLFQV
                     TLIVLAYAVIEGHQFSSYYIFYGTLFFAIVYVSAFRWTFERVTGAVLRAAGYRRRAVL
                     VGSGTHIDAVAHALSDSSEVEPFGYVSLTPRKGDGRLRDFGTLDGLERNFDAVDEVLI
                     ADPAFPQDQAVELVDRCHEHGKRIRVAPSTMEILMDRVEFVPGQSLPLFELKPPVFEG
                     ADFVLKRAFDLVGAVLLLTVLSPLLALIAVAIKLDSPGPVLFRSRRPGIGGGAFDCLK
                     FRTMEEGAEHRQDDLEHRNEAGGAIFKIREDPRVTRVGALLRRWSLDELPQLFNVLRG
                     EMSLVGPRPLPQRDYDLLDDWHRKRYLVLPGMTGLWQVSGRSELDFDELVRLDFLYLE
                     RWSVFLDLTVLLKTIPAVFRRHGAW"
     gene            6666..7385
                     /locus_tag="VHF88_07310"
     CDS             6666..7385
                     /locus_tag="VHF88_07310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2161612.1"
                     /translation="MSEQYTPDNLLDRLSDPATLKLHYDRLRERERALLGARLDLAAG
                     GDVLAVGCGRDPGRHLFPAPAWRMTGVELEEEWPRRLVDSGELDVGLVGRAGELDRLG
                     DASFDVVLYRYVLHHIAFQGPLAPVFAEASRLLRPGGVLVAMEPGALHPVGAGLALAN
                     RIGLGVAVHGTPDDIPLTAARLERESHAAGLDPEVVAVTYGWRRLPPPLQRATWRLDG
                     SLGERPRAARFGHTLMLVARR"
     gene            7396..7974
                     /locus_tag="VHF88_07315"
     CDS             7396..7974
                     /locus_tag="VHF88_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2161613.1"
                     /translation="MPSAYDDDLWRLVPEDAGPPPARLAEFVAGLGRVGSALDLGCGD
                     GRLAALLDAEHLTLADVSDVALQRALRRLPGARAVALEPDAPLPLADNDFDLILCAET
                     VEHVRDVQLLLSEARRVLRPGGRLALTTPAHSRLTGLDVLVRGFERRFPPLSPHLRFL
                     TARSLSELLDDLGFDAGRVRRVDGSLLVVATR"
     gene            complement(7964..9301)
                     /locus_tag="VHF88_07320"
     CDS             complement(7964..9301)
                     /locus_tag="VHF88_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161614.1"
                     /translation="MVAPLIDSPSATAVMTPWGRAAGLRSRQLRPGPGNRPDAVERNQ
                     RERLYGATVAAVAAQGYETTRVADILALAGVSRSAFYHYFDNKLECFLATLDALAEMT
                     RAQVVESYRDAGESWHARLQAVFEGIVATILLQPAAARVWMVEVYAAGPEAVDRMEQV
                     ADQLELLATAAIGETPERSDMPAEGVRAVLGGLRQIVQTRLRHGRAEELPTLVPELLD
                     WALGYSTPPTRLRKPRKPPRLPATVPDPNEQRRKILATVTELVADEGYQAMTVTEISQ
                     RAGVSLTTFYNHFASKREAFMAAIDDGERQLVEIALPAYEQAVDWPHATRDVIHAFFA
                     FNATHPAMAQLGGQRIFSGGAQGFDRHESATGRFGAVLRAGYREYPETSPVVAEAVSG
                     AVAALLYQQIRRRGPESAYEVAGIASFLALAPFVGVDEACTLANQGWRPAVNA"
     gene            complement(9385..9870)
                     /locus_tag="VHF88_07325"
     CDS             complement(9385..9870)
                     /locus_tag="VHF88_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161615.1"
                     /translation="MVMGPDGEKLAERPEAAASLDSRLLAKVDAAFESAARRAQTALE
                     ITDSSDDVADRFESALLAVLEAAASEPDLTRLVLVEAPGLGAPAVERKETGLQRFVDL
                     LDRELAAGNGGAPPPLVAEMVVGGIYEVVQGAARAGDFASMPSLAGELRQLWLPALRG
                     R"
     gene            10096..11127
                     /locus_tag="VHF88_07330"
     CDS             10096..11127
                     /locus_tag="VHF88_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2161616.1"
                     /translation="MSAGGLVESAQASGLAGVSAPARVALAAGVPAAAPIRTEDAILA
                     HAARAGADLVGDACVVWRVLEDGRLEPAATFHRDRRLRYALGAVLHQPPLRAGGLWPG
                     QVATSGAPTRVRRSRLGELAVDTGIGMRRAHALLAPVLVDGRALAVIVALRDSGEPEY
                     SLREEVVMRRVAAKVGARAAAPHGGRGHVGGDEDGGGDRGDGAAGDDGPPSGDGWMPP
                     AAWLMDHIGVGIWVADRDGMTTYVNSAMTELLGVPAADVVGRPMSDFLEDVPQMVRGE
                     YCMDAERCDRRLTQPDGRHVWLEMTSLPLVDDDGARRGVVSTTVDVTARKQVELAARQ
                     RVPRAHRRV"
     gene            complement(11129..11707)
                     /locus_tag="VHF88_07335"
     CDS             complement(11129..11707)
                     /locus_tag="VHF88_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161617.1"
                     /translation="MTDAVGRDGYQAARVADVIAHAGVSRKTFYEHFEDKEHCFIAAY
                     QQALARLLSVTLEAFEAQDEWVDQLRAGLTALLNALAYEPTVARVCFVEVLAAGPNAV
                     ATRNEAMRGFAYIYDSGRLESDGDLPGFTGISMVGGLSEILYREITAGATTELPQLIP
                     ELMYMSVLPFVGADAAARELERGRRRRESSQN"
     gene            complement(11982..12605)
                     /locus_tag="VHF88_07340"
     CDS             complement(11982..12605)
                     /locus_tag="VHF88_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161618.1"
                     /translation="MWDQANIKLTGGPHGIPRPVVTKIQRDRLMRAMAKTVAEYGYQE
                     TTVRRLLGRAGLSRRTYYELFEDKEDCFLAAYDEAVEHVLGLTVEAYHQGESPEAKIE
                     RGLRAFLQFCADEPDIARMCIVEVLAAGPAARARRATTMERLAGLMEGALNELRGDDR
                     LSKLSARGLIGGVHELIYTPIDRGETEHLPDLADQIVSSQIAPLVLV"
     gene            complement(12818..13432)
                     /locus_tag="VHF88_07345"
     CDS             complement(12818..13432)
                     /locus_tag="VHF88_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2161619.1"
                     /translation="MESGIAEAERDALCHALTRVVAEHGWIGATAARLAREAGIEPAR
                     FYDHFRSPEECYVAVFDRMLVRVTRCARRAVASRTLTLGTDAWQEQLDAIFSCVLTFF
                     SVEPALARTCLVEGLSAGPAARARRDDALGRFTGYVEGLRLTHGEPMPPLAAELIVLG
                     TTELIHTRVARGEAEQLPSLLPELRRLWSTTVERQVERPEPLVA"
     gene            complement(13505..>14506)
                     /locus_tag="VHF88_07350"
     CDS             complement(13505..>14506)
                     /locus_tag="VHF88_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161620.1"
                     /translation="VDELTSDSAAPPTGGGRLGSVASERFGYWNEAFDAFGERPLAGL
                     GAGSFELARLAHRNDGGTARRAHGFVAQTVSDLGLAGAIAALTLLAAWLAAATRATAL
                     QPRRWWQAPARARPRWDSERAALVALALAAVVYGVQSALDWTWFVPGLTVSALVAAGF
                     VAGRGPSTAARRPRASAPRLLACAGVVLIAVFCGWAIWQPVAADRATARAYDLIGAGK
                     PLAALRETTRARDLNPYSPQPRYATAVALADLDREQTAIAVLRATAADRPRDPAPWLR
                     IATIELDRRDAPAPALAAAEQALRRDPHSARIVATRDRAAQLLAARQAASAPAPSSP"
BASE COUNT         1921 a         5057 c         5273 g         2255 t
ORIGIN      
        1 catcaggtag cgcgcgtcct cgaggcaggc ctcctcgtcc tcggaggcga agtgcgcgac
       61 gcccgacttc gtgttgtggg tcatcgcgcc gccgagctcc tcgaactcga cctcctcgcc
      121 ggtgacggtc ttgatcacgt cagggcccgt gatgaacatg tgcgaggtct ccttcaccat
      181 gaagatgaag tcggtcatcg cgggcgagta gacggcgccg ccggcgcagg ggcccatgat
      241 cagcgagatc tgggggatca cgccggaaca ctggacgttg cggacgaaga cgtcgccgta
      301 ggcgccgagc gagacgacgc cctcttggat ccgcgcgccg ccggagtcgt tgatgccgat
      361 caccgggcag ccgatcttgg ccgccaggtc catgatcttg cacatcttct cggccatcac
      421 ctcgccgagc gagccgccga agacggtgaa gtcctgcgag aagacgcaga ccgggcgacc
      481 gtcgatcgtg ccgtggccgg tcacgaccgc gtcgccccac ggccggttct tctgcatgtc
      541 gaagtcgaac gtgcggtggc ggacgaaggt gtccagctcc tggaagctgc ccgggtcgag
      601 cagcttctcg acgcgctcgc gggcggtcag cttgccgcgc gcgtgctgct tctcctcggc
      661 ctgcgggttg gtgtggatcg cttgctcgcg cagctcctcg agctgggcga gcttctcctc
      721 gtaggtctcc ggcgacttct ggcgcatagg gcgcggcatc gtatcggcgc gccacggacg
      781 ggcgccgcta ggccgtgcta gccgcgcgag aaggggcggt ggtgtgcctg tccgagcgcc
      841 tcgagggtgc cgtcgaggat ccgctggatc cgctcggcct cggcgttggc caccagcggc
      901 tcgggctcgg gctcgggctg cggctgcggc tccggatcga ccaccggctc gggcgctggc
      961 gtgggctcca gagcaggcac ctgctcgatc gcaacgggcg tcgggtccac ggcgaggcca
     1021 cgcggcgtcc gctgccggaa gagccagcgc gcgaggaagc cctgccgctt gggctccggc
     1081 tcgcgctcga ccggggcggg ctcgaccgcg accggctcgg gctccaccgc gacgggctcg
     1141 accacgaccg gctcgtgctc cacggcgacc gccgccggct ccggctcggc gggcgccggc
     1201 aggtcggccg ggcccggctc ggggacggtc gccgtgaagg tgaccttcgt tccccagccg
     1261 gactgctcga gcgccacggt tccgctcact tcctcgccct cccaggcgac cgtggactcg
     1321 ggctcgacct tcgtgatccg aatctccccc agctcgccga ggtgcttcgc gaggctctcg
     1381 acctcgctca gctcgctcca cagctccggg ggcgacttca ccagcgtgcg ttgtgcggtc
     1441 agatcggcca tcccggtcgg tttcggccgg cggcagacga ctcctgccag ttgggagaga
     1501 cctcccagcg gcctaatgcg gcgcgagggc gaggcgaagg gcgccggcgg cctcgagcat
     1561 cgcgtcgtgc gcgccgcggt ggagggtggt ttggttgacg aaggagtgca gcaggccgtc
     1621 gtggcggcgc agcgcagtcg ggacgccggc ggcgatcagg gcgcgggcgt actcctcggc
     1681 ctcgtcgcga agcgggtcga agccggcggt caggacgtag gcaggcggga ggccggagag
     1741 gtccggggcc tggagtgggg aggcgcgcca ctcgtgggcg gcgtcgcggt cgggcaggta
     1801 gtggtcgcgg aaccaatcca tctcggcggc ggtgagcagg aagccatcgc ggaacaactc
     1861 gtaggagcgc cgcttctcga cgtagtcgca gacgggatag atcaggagct ggaaggcagg
     1921 caacggcgcc tcgccggcct tcgcggcgag cgcgacgact gccgccaggt tgccgccggc
     1981 gctgtcgccg ccgacggcga tccgcgccgg gtcgaagccg agccgcttgg cgttctccga
     2041 cgcccaggcg agcgcggcga tcgagtcgtc gaccgccgcc ggataagggt gctcgggcgc
     2101 cagccgatag tcgattgaga ggaccccggc gctggcgtgc atcgcgagga agcggcaggc
     2161 gctgtcatgg gtgtcgagcg tcccgatcac gtgaccgccg ccgtggtagt agacgagcag
     2221 cccgcccggt gccgccgatg aaggattcgc atcgcctgga ctgtcgaagc cctcagctgg
     2281 gggcgggaat ggcgaataga gccgcgcgcg gatctccccg gccggccctg ggatcgaatg
     2341 gtcctctacg cgcgcgaggg gaagcttggg accggtcagc agcgccgcct gacgtcccac
     2401 ctgcgctcgc gcgtcggcga ccggcaggct ctcgaccggc ggcacgtcgg cgagctgctc
     2461 gagcttgacc agcagctgcg acgtcgggtg cagctcgagc ccgtcgattc ggatcggcgt
     2521 gcccccgagc agccgctgcg cgccaagcgg cagcttcgcc agcgcgacgc agatcgccgc
     2581 ctcgacccgg tcacgcgggg tgaccgcgac cccgctcacc ggctcaggcc tcggagtact
     2641 cgccgaactc gtcgcgcatc acgttgcaga cctcgccgat cgaggcgcgg gccttgagcg
     2701 cggcgcggat cggcgggagc aggttgccgg tgccctgggc ggtggcccgc agctcggcga
     2761 gtgcggagtc gacggccgcc tggtcgcggt tggccttgaa ctccttcagc cgcgcgacct
     2821 gctcgtgctc ggactgcggg tctacgcgca gggtctcgac ctcgtcgacg tcgttggtga
     2881 cgtactcgtt gacgccgacg acgatgtcct gcttgatccg gtagcgctcc tcgtagcccc
     2941 aggccgactg ctcgacctcg gcgcggatga acgggatgca ctggaccgag ccgcccatgt
     3001 cgtcgacctt gtcgatcagc gcctgggcgc cggcctcgat ctcgtcggtc agcgcctcga
     3061 tgaagtacga cccggcgaac gggtcgacgg tgtcggtcgc gcccgactcg gcggcgatga
     3121 tctgctgggt gcgcagggcg atccgagccg ccttctcggt cggcagcgcg agcgcctcgt
     3181 cgtagccgtt ggtgtgcagc gactgcgtgc cgccgcagac ggcggcgaag ccctgcagcg
     3241 cgacacggac gatgttgttc tcaggctgct gggccgccag cgtcacgccg cccgtctggg
     3301 tgtggaagcg gatcgtctgc gacttcgggt tcgtggcacc gaaccgctcg cgcatgatct
     3361 gcgcccacat ccggcgtgcc gcgcggaact tcgcgacctc ctggaagacg ttgttgtggc
     3421 cgttgaagaa gaacgccagg cgtgggccga actcgtcgac gtcgaggccc gcgtcgatcg
     3481 ccgcctggac gtaggcgatc gcgttggcga gcgtgaagcc gacctcctgg accgccgagc
     3541 agcccttctc gcgcatgtgg tacccggaga tcgagatcgt gttccacttc ggcacccgct
     3601 cgcggcagta ggcgaacagg tccgtggtca gccgcatcgc gggctgcggg ggatatatga
     3661 agttcccgcg cgcgatgtac tccttcagga tgtcgttctg ggtcgtgccg cgcaggacgt
     3721 gcgactcgac gccctgctcc tcgccgacca gctcgtagag cagcagcagg caggccgccg
     3781 gcgcgttgat cgtcatcgac gtcgacacct tgtcgagcgg gatgccgtcg aagacgcgcc
     3841 gcatgtcgtc gatcgtgtcg atcgcgacgc cggtgcggcc gacctcgccg aggcagagcg
     3901 ggtcgtcgga gtcgcgcccg agctgggtcg gcaggtcgaa ggccatcgac agccccgtcg
     3961 acccgtggtc gatcaggtag tggaagcgct tgttggtctc ctgcgcggtc gcgtagccgg
     4021 cgtactggcg catggtccac tggcggtcgc ggtacatccg ctcgtgaacg ccgcgcgtga
     4081 aggggtagtc gcctggcttg ccgagcttct ccgggagatc gggtgggagg tcgtcctcgg
     4141 tgtagagcgg cttgatctcg atgccggagt ccgtgaagcg gcgagggtcg tcgggaccga
     4201 cgatcggggc gatcgaaggg cgggcgtcgg gggtggtggc catgggagga ggatacggct
     4261 aggcggcggc cctgagctgg cggaggcgtg cgcggcatgc gaggcacgtc gcgaggtgac
     4321 gacgcaccgg ggcgacctcg tcggaggacg catcgccggc cgcgaaggcg tcgagtgtgg
     4381 tcgcgaggcg gccgcactcc gacccggact cgatgccctc gatcgcctgc cggaaggcat
     4441 ggcggccctc ggcgaggcag cggttgacct tggtcgcgct ccagccggtg atctcggcga
     4501 tctccttgta gctatagccc tcggcctgaa gctgaagcgc cctgatctct tgtggcttga
     4561 gccgacgcag cgcctggctg ccgagccgta ggcgctcgag ctgctcggcg cgggcgtcgg
     4621 tgttcggcgt ctcgtgcgtg gagaacatgc gcgtgcctgt gagcgactcc gccgtgccgc
     4681 cggtgacgat tcgacgacgg tgccgagcga tggcgagtgc ctcgttcttg accgtcgtgc
     4741 gcagccagcc gcgaagctcc tgggcctcgc cggtcggatg cctgcgcagc acacgctcga
     4801 cagcgcgctg gaaggcgtcc tcggcgtcct cgtcggtcgc cgaccagcgc cgcgccgtcg
     4861 ccagcatcga agcgccatgc tcctcgatca accgctctgc gtcgctgccg cgcatgcccc
     4921 gtgccttcct gatcgccgtc cggtccgggc ctagaggggt gtttcggccg tttcccgccc
     4981 cccttcgcgc acatccacag ctacggtgct cgccggagct cgcccccgcg agcactcaac
     5041 gtgccgatag caccgctccg gctgcgccct ccgctacatt ccgttggttg acctgtttgc
     5101 tcttgtggtg cgcagggcgc gaacgagagc tatcggaggc gagctgcagc tgatgggcga
     5161 tggcctggcg agagagctga gagagctgga gccgcgggcg gcgccggcca ggctccccgc
     5221 gcgcgacgtg cgtgccggca gcgtcccgct gctcaagggg gtgctgcgct tcgaagcgct
     5281 gcgacgcggt ggacgcgtgc tgacgctcgc ggcgctcgac gtcgccggaa tgttcctggc
     5341 gatctggacg gcgctcgcga tcaaggcggc cgtgcgcgcg ccgggcatcc tcggcagcac
     5401 cttcgagcag gcgcaggagt acgcgccgtt ggcctgcctg gtgatgctgc tgctgttcgc
     5461 gcggtccggc ctctaccgcg accgcgcgca gcgacccggg ttcaccaacg tcgtctccgg
     5521 gctcttccag gtgaccctga tcgtgcttgc ctacgcggtg atcgagggcc accagttctc
     5581 gtcgtactac atcttctacg gcacgctgtt cttcgcgatc gtctacgtct cggcgttccg
     5641 ctggaccttc gagcgggtca ccggggcggt gctgcgcgcg gccggctatc gccgccgcgc
     5701 cgtactggtc ggctcgggca cccacatcga cgctgtcgca cacgcgctca gcgacagctc
     5761 cgaggtcgag ccgttcggct acgtgtcgct gacgccgcgc aagggcgacg ggcggctgcg
     5821 cgacttcggc acgctcgacg ggctcgagcg caacttcgac gccgtcgacg aggtcctgat
     5881 cgccgacccg gccttcccgc aggatcaggc cgtcgagctg gtcgaccgct gccacgagca
     5941 cggcaagcgg atccgcgtcg cgccgtcgac gatggagatc ctgatggacc gcgtcgagtt
     6001 cgtcccgggc cagtcgctgc cgttgttcga gctcaagccg ccggtcttcg agggtgccga
     6061 cttcgtcctc aagcgggcgt tcgacctcgt cggggcggtg ctgctgctga ccgtgctctc
     6121 gccgctgctg gcgctgatcg cagtcgcgat caagctcgac tcgcccgggc cggtgctctt
     6181 ccgctcgcgg cggcccggga tcggcggcgg agcgttcgac tgcctcaagt tccggacgat
     6241 ggaggagggc gcggaacacc gccaggacga cctcgagcac cgcaacgagg ctggaggggc
     6301 gatcttcaag atccgcgagg acccgcgcgt gacgcgcgtc ggcgcgctgc tgcggcgctg
     6361 gtcgctcgac gagctgccgc agctattcaa cgtcctacgc ggcgagatgt cgctggtcgg
     6421 gccgcggccg ctgccccaac gcgactacga cctgctcgac gactggcacc gcaagcgcta
     6481 cctcgtgctg ccggggatga ccggcctctg gcaggtctcc ggccgctccg agctcgactt
     6541 cgacgagctc gtccggctcg acttcctcta cctcgagcgc tggtcggtct tcctcgacct
     6601 gacggtcctg ctcaagacga tcccggccgt cttccgccgc cacggcgcct ggtgagcgcc
     6661 gcggcgtgag cgagcagtac acgccggaca acctgctcga ccggctgtcc gacccggcga
     6721 cgctgaagct gcactacgac cgcctgcgcg agcgcgagcg cgcgctgctc ggcgcgcgcc
     6781 tcgacctcgc cgccggcggc gacgtcctgg ccgtcggctg cggtcgcgac cccggccgcc
     6841 acctcttccc ggcgccggcg tggcggatga ccggtgtcga gctcgaggag gagtggccgc
     6901 ggcggctggt cgacagcggc gagctcgacg tcgggctcgt cggccgtgcc ggcgagctcg
     6961 accggctcgg cgacgcctcg ttcgacgtcg tgctctaccg ctacgtgctg caccacatcg
     7021 ccttccaggg accgctcgcg cccgtcttcg cggaggcctc ccgcctgctt cgccctggcg
     7081 gcgtgctcgt cgcgatggag cctggcgcgc tgcatccggt cggcgccggg ctcgcgctcg
     7141 cgaatcggat cggcctcggc gtggcggtcc acgggacccc cgacgacatc ccgctgaccg
     7201 cggctcggct ggagcgcgag tcgcacgccg ccggcctcga ccccgaggtg gtcgcggtca
     7261 cctacggctg gcgccgcctg ccgccgccgc tgcagcgcgc gacgtggcga ctcgacggct
     7321 cgctcggcga gcgtccccgg gccgcccggt tcggccacac gctgatgctc gtcgcgcgcc
     7381 gttagcgtct cgcccgtgcc gagcgcctac gacgacgacc tctggcggtt ggtgcccgag
     7441 gacgccggcc cgccgccggc acggctggcc gagttcgtcg ccgggctcgg acgcgtgggc
     7501 tcggcgctcg acctcggctg cggcgacggg cggctcgcgg cgctgctcga cgcggagcac
     7561 ctcacgctgg ccgatgtgtc ggacgtggcg ctgcagcgcg cgctacggcg actgcccggc
     7621 gcgcgagcgg tggctctcga acccgacgcg ccgctgccgc tggccgacaa cgacttcgac
     7681 ctcatcctct gcgccgagac ggtcgagcac gtgcgcgacg tccagctgct gctctcggag
     7741 gcccgccgcg tgctgcgccc cggtgggcgg ctggcgctca cgacaccggc ccattcgcga
     7801 ctgaccggtc tcgacgtgct cgtgcgcggg ttcgagcggc ggttcccgcc gctgtcgccg
     7861 cacctgcgct ttctcacagc gcgctcgctg agcgagctgc tcgacgacct cggcttcgac
     7921 gcgggtcggg ttcgccgtgt cgacggctcg ttgctcgtcg tcgctacgcg ttgactgccg
     7981 gccgccagcc ctggttggcg agcgtgcagg cctcgtcgac gccgacgaat ggcgccaggg
     8041 cgaggaagct cgcgatgcca gccacctcgt aggcgctctc cggcccgcgc cgccggatct
     8101 gctggtagag caacgccgcc accgcgccgc tgaccgcctc ggctacgacc ggtgaggtct
     8161 ccggatactc gcggtagccg gcgcgcagca cagcgccgaa gcgcccggtg gcgctctcgt
     8221 ggcggtcgaa accctgcgcg ccgccggaga agatccgctg gccgccgagc tgcgccatcg
     8281 cgggatgcgt cgcgttgaac gcgaagaagg cgtggatgac gtcgcgggtc gcgtgcggcc
     8341 agtccaccgc ctgctcgtac gccggcagcg cgatctcgac gagctggcgc tcgccgtcat
     8401 cgatcgccgc catgaacgcc tcgcgcttgc tggcgaagtg gttgtagaag gtggtcaggg
     8461 agacccccgc acgctggctg atctcggtga cggtcatcgc ctggtagccc tcgtcggcca
     8521 cgagctcggt gaccgtcgcg aggatcttgc gccgctgctc gttcgggtcg gggacggtgg
     8581 cgggtagtcg tggcggcttg cgcggcttgc gcaggcgcgt cggcggagtg ctgtagccga
     8641 gcgcccaatc gagcagctcc ggcacgagcg tcggcagctc ctccgccctg ccatgtcgca
     8701 ggcgggtctg gacgatctga cgcaggccgc cgagcaccgc ccgtacgccc tcggccggca
     8761 tgtcggagcg ctcgggcgtc tcgccgatcg ccgcggtggc cagcagctcg agctggtcgg
     8821 cgacctgctc catccggtcg accgcctccg gcccggctgc gtagacctcg accatccaca
     8881 cgcgtgcggc ggccggctgc aggagaattg tcgcgacgat gccctcgaag acggcctgca
     8941 ggcgggcgtg ccacgactcc ccggcgtcgc ggtaggactc gaccacctgg gcgcgggtca
     9001 tctcggcgag cgcgtcgagg gtcgccagga agcactcgag cttgttgtcg aagtagtggt
     9061 agaaggcgct gcgcgacacg cccgcgagcg cgaggatgtc cgctacccgg gtcgtctcgt
     9121 agccctgtgc ggcaacggcg gcgaccgtgg ccccgtagag ccgctcgcgc tggttgcgct
     9181 ctacggcatc cggtcggttg cctggccctg ggcgcagctg tcgcgagcgc agcccagcgg
     9241 cccgtcccca cggtgtcatg accgccgtcg cgctagggct gtcgatcagc ggggcgacca
     9301 taattggtca gaccacttta cgaacaaagg gctctgccat caagcgccgt ctggcttgag
     9361 cagaggggcc tatgagggtt gtggtcagcg gccgcgcagc gcgggcagcc aaagctggcg
     9421 cagctcaccg gcgagactcg gcatgctcgc gaagtcgcct gcgcgtgcgg cgccctgcac
     9481 cacctcgtag atcccaccga cgaccatctc cgcgaccagc ggcggtggcg cgccgccgtt
     9541 gccggccgcc agctcgcgat cgagtaggtc gacgaagcgc tgcaggcctg tctctttgcg
     9601 ctccaccgcg ggggccccga gcccgggcgc ctcgaccagc acgagccggg tgaggtcggg
     9661 ctcggacgcg gcggcctcta gcaccgcgag cagtgctgac tcgaaccggt cggcgacgtc
     9721 atcgctcgaa tcggtgatct cgagggcggt ctgcgcccgt cgagctgcgc tctcgaaggc
     9781 cgcatcgacc ttggcgagca gccgagagtc gaggctggcg gctgcctcgg gccgttccgc
     9841 gagcttctcg ccatcaggac ccataaccat aaaccgtaca gtttataaaa gcgcgtgtcc
     9901 agtctttcgg gcggccgtcc tgctttctgc ggtacggctg cggctccggt gaacaggccc
     9961 gtgtctcacg acggtgtatc ccccccaagc ggggggttgc tgccgtaaaa cgctcgactt
    10021 acggtggctg aaccgttggg ccgcctggtc cgaaaggcaa accgcctagg tcctcggtct
    10081 ggaaaggagt tccgcatgag cgcaggtgga ttggtcgaat cggcgcaggc gagcggcctc
    10141 gccggggtgt cggctccggc acgggtggcg ctcgccgctg gcgtgccggc cgcagcgccg
    10201 atccgcaccg aggacgccat cctcgcgcat gccgcgcggg ctggcgccga cctggtcggc
    10261 gacgcctgcg tcgtctggcg ggttctggag gacggccggc tcgaacccgc cgcgaccttc
    10321 catcgcgatc gtcgtctgcg ttacgcgctc ggggccgtct tgcaccagcc gccgctccgc
    10381 gccggcggcc tttggcccgg tcaggtcgct accagcggcg caccgacgcg cgtccgccgc
    10441 agccgcctcg gcgagctcgc cgtcgacacg gggatcggca tgcggcgtgc ccatgctttg
    10501 ctcgcgccgg tgctggtcga cggccgggct ctcgccgtga tcgtcgcgct gcgtgactcc
    10561 ggcgagccgg agtactcgct gcgcgaggag gtcgtgatgc gtcgtgtagc cgcgaaggtg
    10621 ggggcccgcg cggcggcccc gcacggcggc cgcggccatg ttggtggcga cgaggacggc
    10681 ggcggcgatc ggggcgacgg ggcggccggc gacgatggcc cgccgtccgg cgacggctgg
    10741 atgcccccgg ccgcgtggtt gatggatcac atcggggttg ggatctgggt ggctgaccgc
    10801 gacggcatga cgacctacgt caacagcgcg atgaccgagc tgctcggggt accggccgca
    10861 gatgtcgtgg gccggccgat gagcgacttc ctcgaggacg tgccgcagat ggtgcgcggc
    10921 gagtactgca tggacgccga gcgttgcgat cgccggctga cccagcccga cgggcgtcac
    10981 gtctggctcg agatgacctc gctgccgctc gtcgacgacg atggcgcccg tcgcggcgtc
    11041 gtgagcacga cggtcgacgt gacggcgcgc aagcaggtcg agctggcggc acgtcagcgc
    11101 gtaccgcgcg cgcatcgccg cgtctgagct agttctggga gctctcgcgg cggcggcgac
    11161 cgcgctcgag ctcgcgtgcg gcggcatccg cgccgacgaa tggcagcacg ctcatgtaca
    11221 tcagctcggg gatcagctgc ggtagctcgg tggtggcacc ggcggtgatc tcgcggtaga
    11281 ggatctcgct gagcccgccg accatgctga tgcccgtgaa gcccggcagg tcgccgtcgg
    11341 actccagtcg gccgctgtcg tagatgtagg cgaaaccgcg catcgcctcg ttgcgggtcg
    11401 cgacggcgtt cgggccggcg gcgagcacct cgacaaagca gacgcgcgcc accgtcggct
    11461 cgtatgcgag tgcattgagc agcgccgtca ggccggcgcg cagctgatcg acccactcgt
    11521 cctgggcctc gaacgcctcg agcgtcaccg acaggagtcg ggccagcgcc tgctggtagg
    11581 cggcgatgaa gcagtgctcc ttgtcctcga agtgctcgta gaaggtcttg cgcgagacgc
    11641 ccgcgtgggc gatgacgtcg gcgacgcgag ccgcctggta gccatcgcgg ccgaccgcgt
    11701 cggtcatcgc cgcgaggatt cggctgcgct gcgactccgc gacctgctcc ggagcgagcc
    11761 catggcgacc gcccgggatc tggtccggtc ggcggcgggc aggtcgccgg ggagtgcctc
    11821 cgcttcccga gcgggcgccc ttcctagagg ccacggccgt ccttcgcgag agcgggctgc
    11881 acgccgacag tctaccgcca ccggacaccc tgtgggtacc caaatggtac gccgctgtgt
    11941 ccggctcggg gctctctaga gcggaaccgt tagttctacg tctaaaccag gaccagtggt
    12001 gcgatctgac tcgagacgat ctggtcggcg aggtcgggca ggtgctcggt ctcgccgcgg
    12061 tcgatcggcg tgtagatcag ctcgtgtacc ccgccgatca gcccacgggc ggagagtttc
    12121 gacagtcgat cgtccccgcg cagctcgttg agcgcgccct ccatcaggcc cgcgaggcgc
    12181 tccatcgtcg tagctcgccg agcacgggcc gccggcccgg cggccagcac ctcgacgatg
    12241 cacatccgcg cgatgtccgg ttcgtcggca cagaactgga ggaaggcgcg cagtccgcgc
    12301 tcgatcttcg cctcgggcga ctcgccttgg tggtatgcct cgaccgtcag gccgagcacg
    12361 tgctcgaccg cctcgtcgta ggcggcgagg aagcagtcct ccttgtcctc gaacagctcg
    12421 tagtaggtcc gtcgcgagag tccggcgcgt ccgagcaagc gacgcaccgt ggtctcctgg
    12481 tagccgtact cggccacggt cttcgccatc gcgcgcatga ggcggtcgcg ctggatcttc
    12541 gtgacgaccg gacgtggaat cccgtgcggt cccccggtca gcttgatgtt cgcctggtcc
    12601 cacatgggcc caccatagac gccgagttct ctcgacgcaa ccataaattt cgagtttcta
    12661 ggtacatcga cgttctccag ttgcctcccc gtccgcctcg agacatcgcg cacacggctc
    12721 tccaaagcgg cagtggtgcg gtattcgcgg ggccctgatt caggcccgca ggagggcgct
    12781 aaccagacac cgacaagtat ctgtacggcc tgacgcttca ggcgacgagc ggctcgggcc
    12841 gctcgacctg ccgctcgacc gtggtggacc agagccggcg cagctccggc agcaggctcg
    12901 gtaactgctc cgcctcgccg cgtgcaacgc gggtgtggat cagctcggtc gtgccgagca
    12961 cgatcagctc ggcggcaagg ggaggcatcg gctctccatg cgtgagccgc agcccctcga
    13021 cgtatccggt gaagcgaccg agcgcgtcgt cgcggcgagc ccgcgccgcc ggaccggccg
    13081 acagtccctc cacgaggcag gtccgcgcga gcgccggctc gacggagaag aaagtcagca
    13141 cgcagctgaa gatggcgtcg agctgctcct gccaggcatc tgtcccgagc gtcaacgtgc
    13201 gcgatgcgac cgcacgtctc gcgcagcgcg tgacgcggac gagcatccgg tcgaagactg
    13261 cgacgtagca ctcctcgggg ctgcggaagt ggtcgtagaa gcgcgctggc tcgattcccg
    13321 cctcacgcgc gagacgcgcg gctgtcgcgc cgatccagcc gtgctcggcg accacgcgcg
    13381 tgagcgcgtg gcacagtgca tcccgctcgg cttctgcgat cccgctttcc atccgtgacg
    13441 ctccgctcgt catcggcgtt cccctgctcg gccgagcgag cataccgccg cgtggaggcc
    13501 gcggctacgg actgctgggc gcaggagcgc tcgcggcttg gcgtgccgcg agcagctggg
    13561 ccgcgcggtc gcgcgtcgcg acgatccgtg ccgagtgagg atcgcggcgc agcgcctgct
    13621 cggccgccgc aagcgctggc gcgggcgcgt cgcggcggtc gagctcgatc gtggctatcc
    13681 gcagccaggg agcggggtcg cgcggccggt cggctgcagt tgcgcggagc acggcgatcg
    13741 cggtctgctc gcggtcgagg tcggcgagcg cgaccgcggt cgcgtagcgc ggttgggggg
    13801 agtacgggtt gaggtcgcgt gcgcgggtgg tctcgcgtag cgcggcgagt ggcttgccgg
    13861 cgccgatcag gtcgtaggcg cgcgcggtcg ctcggtcggc ggcgaccggc tgccagatcg
    13921 cccagccgca gaagacggcg atcaggacga cgcccgcgca ggcaagcagc cggggcgccg
    13981 aggcccgcgg gcgacgcgcg gcggtcgagg ggcctcggcc ggcgacgaag ccggcggcca
    14041 ccagtgcgct gaccgtcaac ccgggaacga accaggtcca gtcgagagcc gactgtacgc
    14101 cgtagacgac ggccgcgagc gcgagcgcga cgagcgccgc gcgttcgcta tcccagcgtg
    14161 gacgggcgcg cgccggcgcc tgccaccaac ggcgcggttg cagcgcggtc gcgcgcgtcg
    14221 cggcggccag ccaggcggcg agcagcgtca aggcggcgat cgcaccggcc aagccgaggt
    14281 cgctgaccgt ctgagcgacg aagccgtggg cgcgccgggc cgtaccgcca tcgttgcgat
    14341 gcgctagccg cgcgagctcg aacgacccgg ctccgagccc ggcgagcgga cgctcgccga
    14401 atgcatcgaa cgcctcgttc cagtagccga agcgctccga tgccaccgac ccgaggcgcc
    14461 cgccgccggt cggcggcgca gcgctgtcgc tggtcagctc gtccac
//
LOCUS       DATABV010000169        14896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2783887, whole genome shotgun sequence.
ACCESSION   DATABV010000169 DATABV010000000
VERSION     DATABV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14896
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783887"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VHF88_07355"
     CDS             complement(<1..333)
                     /locus_tag="VHF88_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX2161621.1"
                     /translation="MAHKELKYDQEARAALQAGVDAVANAVKVTLGPKGRYVVLDKKF
                     GAPTITNDGVTIAREIEVEDVFENQGAQLVREVATATNDVAGDGTTTATLLAQTIVRQ
                     GLKNVAAGA"
     gene            complement(386..676)
                     /gene="groES"
                     /locus_tag="VHF88_07360"
     CDS             complement(386..676)
                     /gene="groES"
                     /locus_tag="VHF88_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX2161622.1"
                     /translation="MKLKPLGDRLIVQTIEEDETTASGIVLPDTAKEKPSRGKVVAVG
                     DGKWDEDGEKRIPLDVSEGDEVLYSKYGGTEVTVEGEELLVLRESDVLAKVA"
     gene            809..1702
                     /locus_tag="VHF88_07365"
     CDS             809..1702
                     /locus_tag="VHF88_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161623.1"
                     /translation="MASRRFPVLLAAAALCLVPAACGSDDDESSGGGSPESAETAAVT
                     FETTEPSRNKVAIDGPSEIEAGVVEITLKNSGKGRHDAQILRVEGNRTADEVISKSID
                     SGEGAGIPEWITDGGGLGTVAPGESATVTEVLEPGTYFLLDSESSAENAPPNARKGGV
                     AKFEVTGEATSAELPETDATITAKDFSFEASGIKPGMNRFTFENTGKELHHLIAMPIN
                     EGATFAEVEKLFKSNEEPKGPPPVDFENGVGTAVIDGGQAQIAELEFKKGKYALVCFI
                     TNRAGGPPHVAMGMIDELDVK"
     gene            complement(1737..2135)
                     /locus_tag="VHF88_07370"
     CDS             complement(1737..2135)
                     /locus_tag="VHF88_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX2161624.1"
                     /translation="MFVLNFYSPVFIDQLKRGRKTATIRLGDKSAKYRKGEVVLVTVG
                     FQHAPRERIFEAVIDGVEVKRVSELSPRDVEHDNPEFRRLDETVHFLEQIYGRKVEQD
                     DIVTVVRFSQISDRPSEITERLRPDAPSQN"
     gene            complement(2174..3466)
                     /locus_tag="VHF88_07375"
     CDS             complement(2174..3466)
                     /locus_tag="VHF88_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161625.1"
                     /translation="MTVTPWGDSRELRARKLRPGPGVPRADVEANQRERLYGAMVTAV
                     ADHGYEATRVADVLEISGVSRNTFYRHFDNKLDCFLATMDAIAVGGGDAVVSAYRQHD
                     GSWDERLAAALDVLVAGILAQPAASRLYYVESYAAGPRAIAKVEQMGEQLEQLARHAL
                     DASPEHRGLPRDLLRAILRGFRRVFQTRLRSGREHELPEIAPQVLDWALGYTTPPEPL
                     RQRSEPSRALFEPPAAADPADPRERILDAVVALMAERGYTGLTINDIAQRAGVSLTTF
                     YDRFDGKDEAIVAALRHCGEQVLAAVRPAFQAAPDWPRGAAAAIDSFFAYLAIEQPFA
                     QFGGVDVHLSSRSIVAVREEMLAGSQAFLADGFRAHPETPAIAGEAIGAAIDALLFDQ
                     IANWGSDRLYELAPIATYLVLVPFVGVEQACAIANSGP"
     gene            complement(3548..4240)
                     /locus_tag="VHF88_07380"
     CDS             complement(3548..4240)
                     /locus_tag="VHF88_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2161626.1"
                     /translation="MLEGGDTAELLASVKVFSGLEPRELEEVARVAVPRSFERGEVIF
                     REGDPGDTCYVVRSGTVSIRRDHPDGRSRALAELRHGAIFGELSMFGAETRSATAEVL
                     EETSLVAILRADMQRVIRSSPDIAFKMLAELADRVRLVNEQLSRQSFQTVSGRVASTL
                     LAQVVTRQSEGAGSHDVLIKATQAEIAQLAGTSRESASRFLSTLAREGVVTLGRGKVT
                     VHDPQRLHGFIH"
     gene            4320..4862
                     /locus_tag="VHF88_07385"
     CDS             4320..4862
                     /locus_tag="VHF88_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161627.1"
                     /translation="MRWVPLALLLAAVAVLVSSAEAHAVPKKAPWATVNVCDTDGKRN
                     SVGIRAGMPGNGTRQRMYMRFQLQWYRPKERRYVDTGAPSTWVSAGSARFRAAQRGFT
                     FRGIADPPAGARFKLRGEVTFQWRVLRPVRKGSKRKRERVVKRARRFTRGGLRGVVGG
                     KPPGRSEAACLVYGPKTSRR"
     gene            complement(4835..5479)
                     /locus_tag="VHF88_07390"
     CDS             complement(4835..5479)
                     /locus_tag="VHF88_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161628.1"
                     /translation="MRSRSAAALAASALVAAFPGAAALAADPPSLRTTYPCYSSGESV
                     LLTGSGFTPDGEVALSLSGQQLTTLDADTDGGFSLRLQAPDALFGTTSLRFTATDRAQ
                     PRLSDHATLRIADSDVLVTPMAGTPEQPRRIRAWGFFGSHAFYAHVKRRGARRARNIY
                     LGTPRGPCGVLDVERRLFRRTPRQGAYVLQFDALRRYYPNLESSVTYRLDVLGP"
     gene            5812..10779
                     /locus_tag="VHF88_07395"
     CDS             5812..10779
                     /locus_tag="VHF88_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161629.1"
                     /translation="MRSGLGDELRMRNGDRGAGASMLGVPVRTLALGLALAIVASLFA
                     AVVAFGSKGGDSFVGVPTASSGEAADGGLFNNPRDIAVNQTGAGGVDPGTVYVVDQSN
                     HRIQRLDADGNFELTWGRDVVTAGAANDVGTTAFEVCDVTHPTTPNTAANCKGGTTGS
                     LGGLLNNPAGVAVDQDTGDVFVTDRGNFRVQQFEADGTFVRAWGWDVIANGSPSDATN
                     VTTFQVCTVAADCKAGTTGAGEGQFGSGTSNWRLDVSPGDGDPASGKVVVADPGSGST
                     GNRRIQIFELADLATGAAPTVDDGEIVGGGAASPFFGGNPQNVAVDAAGIVYGIDVGD
                     VTVKRYDTASGSFLAPISGQRFNELYPGDLAGAFAVPETLEVDPETGNLFVTVNPNIG
                     AAATVIYEFAVPGGGSPSIVDFHMRDAGDQTTNGLGILHSGALDRLYVSASVGTIGQR
                     ILILDENGAEPPPSATIALPTGVGAHEATLNGTVNPNGPTGFSTKYHFEYSKDGSTWT
                     AAAAPVDLGDGSADIVVDDSVTGLEANTLYRARLVTTRTFDAGTTISAETTFTTPAVA
                     PEVTTGPVQRRTATTVELTGRINPNNLPTSYHFEYGPSAAYGFKAPIPDGDAGSAGTE
                     APFVEAIEGLQAGASYHYRLVATNAEGTAFGDDRTFSTRASATPPAGRAYEMVTPADK
                     TNRRTGEFAATEQGLVNPGTPSKDGESLLFRLRYGILDPEAGTDFPHSVDTTIVRRGS
                     DGWAGESVTNVVSPRGATGSLESLQAVSADLDTLAWHHAAELFQSGSSFSTKLMGDTG
                     GLDGSGWYEWVMAPPLGATIDSFTDDAALIDDEGARMVRWGQYRGLLGPDDPSHPDYP
                     NPENPGHSADAFTQTSGSAIYRQEPVGSGPRHLVNECTGTGSTATLIPSRNDNGTVPG
                     PASALGNVTFTAGSAEVTVNSMLVGTFGVGHRVVAVDPALPAGTVPANTFITAVGAGT
                     FTMSAAAGNSATGVVQGQQNSAATNDDFIDAQPCEAGSVTSPRGAAVGAGGADSGSPS
                     IYGPVATAMSRDGSRVFFTSPDPRVTAGVPVTCGTGSFRLTDCPPQLFVRQYDADGDA
                     TVRWISRSEVDGQAIGLMGAGAIFQGASADGRYVYFKTNAPLVEDDPNGGAITPGGVT
                     TGTASPSSWDIYRYDLTANTSADPADGALMRISGGPSGTADPNTNPVPSDSTVGSGSS
                     VRYVSDDGARVYFVTGAPIGAADDPWNEGPSGTEPGTASSTRNLYVFDATESGEARWR
                     FIAQIPYSVEGGVLSDVANCAGSGSESGYANVFSSTSNVNHQREAVNCVRGTRDGSAI
                     AFETPGRLTEDDTDSASDIYLYDERSHELTRITTPPLGSAPYACDHDPLDRPVAFCNG
                     DFGFPSPPGAPSRPAGQERVGLDGRHHDNLAIDESGELSVYFESRVPLVPEDTNGNHM
                     DTYEWRAGELSLISPGKTSDDAWYSGNSADGQDVFFFTSQRIDPREIDDGDLDVYDAR
                     VGGGFPPPPPRPQGCDSLADGCQGPGGGPPLAGDPQTDRPGGGNATPDRRARLRAVRP
                     GRRALRRAARTGVIRLRVRTNTAGRVSATARARVKVGRRSRTIVVARRSIRVGKPGAR
                     VLRLRLKKSARRHLRTGRPLRVRLRVAQADAEPRRVKVVLRRAGR"
     gene            10986..14099
                     /locus_tag="VHF88_07400"
     CDS             10986..14099
                     /locus_tag="VHF88_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161630.1"
                     /translation="MGFGIADAVGAAWPGTSSAAWWAGTCDLDEASTSGVGVGTPPAS
                     FAHCIDHGTPYDPVGETPTPPRETTWQPGGEPSWRLDPVAQAGAHPDATASFWLQRSP
                     HPALYGSAPVTLQGEGEARTVKVSLPPGVVGDPNAVPRCPSKAMQTTPTTCPPETQVG
                     VATVTLVQTGTPPYMQSLYPVYNAEPRDGKTAELIVSVATNNAKANIPIVARARTEGD
                     FGVDGLAVDLPGGIPVLGQTITLWGVPWASSHDKFRPRAGYAGHQYAGGSAGGMPETG
                     LTNSDPTQEPQSYDPSWGPIRPFFTNPTECAPAPPVTELGIASWQDPDRMLTYPAFAD
                     ALVARCGAVPFDADIDVRPTTTAADAPTGIEADLRIPQNNDPPAAVATNPDDATGAPA
                     HWESDAGLGTAQLDRAVVTLPRGVSVNPSGAAGLEGCSDAQIGLTRNGNPPLFNNEDP
                     FDGRGAECPDGSKIGTVDVYTPLLPAAYGEVPGTPNLRGEVVLGMPRSTDPTSGDMFR
                     MFVVVRNVKRGLVAKVFGSAVADPATGQLTATFDRNPRVPFETMQLRFKGGDRGLVAT
                     PQRCATHGWATTLTPWTAAHGGGGASVADGGAFTTNANCGYGFSPALAAGMSERRGRA
                     NGTFSLTVARADGQQWLAGLSAEMPTGLLANVGDVPLCGDAQANAGACPIASRIGTVD
                     AAAGAGSPFVLERKGDVYLTEGYKGAPYGLATVVPVEAGPFRGRFALTPVVVRQALHV
                     DRTDASVTAVSDPFPQIWHGIPLRVRRVTISIDRPGFMLNPSDCSPKAVAATIASSEG
                     AAATPAVPFQAIGCGSLRFKPRLGMRLTGKRQRVTGRHPGVRAVVRQRAGEAGIERAV
                     VRLPKSLALDPDNARALCEFEDGTKPDLERHCPKGSIVGRARAISPLLRRPLVGNVYF
                     VKNVRIDRDTGNEIRTLPMIVVALRGEIAVNLRGESSVRGKRLVNTFAGVPDAPVSRF
                     NLNIRGGRNGILAVTRTRKARINLCSARRQIAEADMDGHNGRRHDRNVRIKVPCPKRR
                     ATGKRRR"
     gene            14252..14842
                     /locus_tag="VHF88_07405"
     CDS             14252..14842
                     /locus_tag="VHF88_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161631.1"
                     /translation="MRKFMLVAAAALASMATLTATATAQTPVEVHDPSTGEQCYYVAV
                     IAGQVSGGCESRKVANDLVDLGYELPPSWGGGWMHTDSGGLAFDVNVGSYGDIYISNI
                     AFTGWASAASACRGSEPWTGEIMRTPAGDLEADVDICYSHMFGTADGNVTFRLDDGDA
                     WTALIADRQVVGGGTHGPTFEGHFVGGSPLQVIDAQ"
BASE COUNT         2301 a         5042 c         5332 g         2221 t
ORIGIN      
        1 ggcgccggcg gccacgttct tcaggccctg gcggacgatc gtctgggcca gcagggtggc
       61 ggtggtggtg ccgtcaccgg cgacgtcgtt ggtcgcggtc gcgacctcgc ggacgagctg
      121 ggcgccctgg ttctcgaaga cgtcctcgac ctcgatctcg cgggcgattg tgacgccgtc
      181 gttggtgatc gtcggcgcgc cgaacttctt gtccagcacg acgtagcgac ccttggggcc
      241 gagggttacc ttgaccgcgt tggcgacggc gtcgacgccg gcctgcagcg ccgcccgagc
      301 ctcctggtcg tacttgagct ccttgtgagc catttcgttc ctccgttgtt cctaagaagt
      361 ttccggtccg cgctgccctg tggggctagg cgaccttggc gagcacgtcg gactcgcgca
      421 ggacgagcag ctcctcgccc tccacggtga cctcggtgcc gccgtacttg gagtagagga
      481 cctcgtcgcc ctccgagacg tcgagcggga tgcgcttctc gccgtcctca tcccacttcc
      541 cgtcgccgac cgcgacgacc ttgccgcgcg agggcttctc cttggcggtg tccgggagga
      601 cgatgccgct ggcggtcgtc tcgtcctcct cgatggtctg gacgatcaag cgatcgccga
      661 gaggcttgag cttcatcagg ggtgttacct ccgtctacga ctgagtccgt tgaacttctt
      721 tggcactcta ccaagccgag tgccagggtt tgccttccgg ccgctccagg taggaggatg
      781 tccgcaatca acgagaggag tcgcctccat ggcgtctaga cgcttccctg tcctgctcgc
      841 cgcagccgcg ctgtgcctcg tgccggccgc ctgcggctcc gacgacgacg agtcgtcggg
      901 cggcggctcg ccggagtccg ccgagaccgc tgccgtcacc ttcgagacca ccgagccgag
      961 caggaacaag gtcgcgatcg acggtccgag cgagatcgag gccggcgtag tcgagatcac
     1021 gctgaagaac tccggcaagg ggcgccacga cgcccagatc ctgcgcgtcg agggcaaccg
     1081 gacggccgac gaggtgatct ccaaatcgat cgactccggc gagggcgccg gcatccccga
     1141 gtggatcacc gacgggggcg ggctcggcac cgttgcgccg ggcgagagcg cgacggtgac
     1201 cgaggtgctc gagccgggca cctacttcct gctcgacagc gagagcagcg ccgagaacgc
     1261 gccgccgaac gcccgcaagg gcggtgtggc gaagttcgag gtcaccggcg aggcgacctc
     1321 agccgagctg ccggagaccg acgcgacgat caccgcgaag gacttcagct tcgaggcgtc
     1381 ggggatcaag cccgggatga accgcttcac gttcgagaac accggcaagg agctccacca
     1441 cctgatcgcg atgccgatca acgagggggc gaccttcgcc gaggtcgaga agctcttcaa
     1501 gtcgaacgag gagccgaagg gcccgccgcc ggtcgacttc gagaacggcg ttggcaccgc
     1561 ggtgatcgac ggcggtcagg cgcagatcgc cgagctcgag ttcaagaagg gcaagtacgc
     1621 gctggtctgc ttcatcacca accgcgcggg tggcccgccg cacgtcgcga tggggatgat
     1681 cgacgagctc gacgtcaagt agcgtcggtc cgtctagcgc ttcggacgct ccgtcttcag
     1741 ttctgcgacg gggcgtccgg gcgcaggcgc tcggtgatct cggacgggcg gtcggagatc
     1801 tgggagaagc ggacgaccgt gacgatgtcg tcctgctcga ccttgcggcc gtagatctgc
     1861 tcgaggaagt ggacggtctc gtcgaggcgg cggaactcgg ggttgtcgtg ctcgacgtcg
     1921 cgcggagaga gctcgctgac gcgcttgacc tcgacgccgt cgatcaccgc ctcgaagatt
     1981 cgctcgcgcg gggcgtgttg gaacccgacc gtcacgagca cgacctcgcc cttgcggtac
     2041 ttggccgact tgtcaccgag ccggatcgtc gccgtcttgc gaccgcgctt cagctggtcg
     2101 atgaagacgg gcgagtagaa gttgaggacg aacaccgcgg gcagcctagc ggaggcgggc
     2161 ccgcgaatcg ctgctagggg ccggagttgg cgatcgcgca ggcctgctcg acgccgacga
     2221 acggcacgag cacgaggtag gtggcgatcg gggcgagctc gtagaggcgg tcgctgcccc
     2281 agttggcgat ctggtcgaac agcagcgcgt cgatcgccgc cccgatcgcc tcgccggcga
     2341 tcgccggggt ctcggggtgc gcgcggaagc cgtcggcgag gaacgcctgg ctgccggcga
     2401 gcatctcttc gcgcaccgcg acgatcgagc ggctgctgag gtggacgtcg acgccgccga
     2461 actgggcgaa cggctgctcg atcgcgaggt aggcgaagaa ggagtcgatc gccgcggcgg
     2521 cgccgcgcgg ccagtcgggc gccgcctgga aggccggcct gaccgcggcg aggacctgct
     2581 caccgcagtg cctgagcgcg gcgacgatcg cctcgtcctt gccgtcgaag cggtcgtaga
     2641 acgtcgtcag cgacaccccg gcgcgctgcg cgatgtcgtt gatcgtcagg cccgtgtagc
     2701 cgcgttcggc catcagcgcg acgacggcgt cgaggatccg ctcgcgcggg tcagcggggt
     2761 cggcggccgc cggcggctcg aacagcgcgc gcgacggctc gctgcgctgg cgcagcggct
     2821 ctggcggcgt cgtgtagccg agcgcccagt cgagcacctg cggagcgatc tccggcagct
     2881 cgtgctcgcg cccactgcgc aggcgggtct ggaagacgcg ccgaaacccg cgcaggatcg
     2941 cccgcagcag gtcacggggg aggccgcggt gctcgggcga ggcatcgagc gcatgacggg
     3001 cgagctgctc tagctgctcg cccatctgtt cgaccttcgc gatcgctcgc ggtccggcgg
     3061 cgtagctctc gacgtagtag agccgcgacg cggccggctg ggcgaggatg ccggcgacga
     3121 ggacgtcgag cgccgcggcg agccgctcgt cccagctgcc gtcgtgctgg cggtaggcgc
     3181 tgacgaccgc atcgccgccg ccgacggcga tcgcgtccat cgtcgcgagg aagcagtcga
     3241 gcttgttgtc gaagtgcctg tagaaggtgt tgcgcgagac gccggagatc tcgagcacgt
     3301 cggcgacgcg cgtcgcctcg tagccgtggt cggcgacagc ggtgaccatc gccccgtaca
     3361 ggcgctcgcg ctggttggcc tcgacgtcgg cacgcgggac gcccggtccc ggacgcagct
     3421 ttcgtgcgcg cagctcgcgc gagtcccccc acggcgtgac ggtcaccggg cgaaccatat
     3481 tctgaaaacg cccgtggcta tgtctgccag tacgagcgcg tctccggggc ggcggggttt
     3541 gacgcggcta gtggatgaag ccgtgcagcc gctgcgggtc gtgcacggtc accttgccgc
     3601 gcccgagcgt gaccacgccc tcgcgcgcga gcgtggagag gaagcggctg gcgctctcgc
     3661 gcgaggtgcc ggcgagctgg gcgatctcgg cctgggttgc cttgatcagc acgtcgtggc
     3721 tgccggcgcc ctccgactgg cgcgtgacga cctgggcgag cagggtgctg gcgacgcggc
     3781 cggagacagt ctggaacgac tggcggctga gctgctcgtt gacgaggcgg acgcggtcgg
     3841 cgagctcggc gagcatcttg aacgcgatgt cgggcgagga gcggatcacg cgctgcatgt
     3901 cagcgcgcag gatcgcaacg aggctcgtct cctcgagcac ctcagcggtg gcggagcgcg
     3961 tctcggcgcc gaacatcgac agctcgccga agatcgcccc gtggcgcagc tcggcgagcg
     4021 cgcggctgcg gccgtcgggg tggtcgcgac ggatgctgac cgtcccggag cggacgacgt
     4081 agcacgtgtc gcccgggtcg ccctcgcgga agatcacctc gccgcgctcg aagctgcgcg
     4141 ggaccgcgac gcgggcgacc tcctccagct cgcgcggctc gaggcccgag aagaccttga
     4201 cgctggcgag cagctcagcg gtgtcgccgc cctcgagcat tggaggatga taggccggta
     4261 ctcgccaacc gccggccgaa tggcgcgacg ccggcggatc ctctctacga tcgagcgcga
     4321 tgcgctgggt cccgctcgcc ctcctgctgg ccgccgtcgc ggtgctcgtc tcgagcgccg
     4381 aggcgcacgc cgtgccgaag aaggcgcctt gggcgacggt caacgtctgc gacaccgacg
     4441 gcaagcgcaa ctcggtcggg atccgggccg ggatgcctgg caacggcacg cgccagcgga
     4501 tgtatatgcg cttccagctg cagtggtacc ggccgaagga gcgccgctac gtggacaccg
     4561 gcgcgccgtc gacgtgggtg agcgctgggt cggcgaggtt tcgggcggca cagcgggggt
     4621 tcacgttccg cgggatcgcc gacccgccgg ccggggcgcg gttcaagctg cgcggcgagg
     4681 tgaccttcca gtggcgcgtg ctgcggccgg tgaggaaggg ctcgaagcgc aagcgcgaga
     4741 gggtcgtgaa gcgcgcgcgg cggttcacgc gcggagggtt gaggggcgtc gtcggcggga
     4801 agccgcccgg gcgctccgaa gcggcgtgct tggtctatgg gcccaagaca tccaggcgat
     4861 aggtgacgct cgactcgagg ttggggtagt agcgccgcag ggcgtcgaac tgcaggacgt
     4921 acgccccttg tcgcggcgtg cgccggaata ggcgccgctc gacgtcgagc acgccgcacg
     4981 ggccgcgcgg cgtgccgagg tagatattgc gcgcgcgcct cgcgccacgg cgcttgacgt
     5041 gggcatagaa ggcgtggctg ccgaagaagc cccacgcgcg gattcgccgc ggctgctcgg
     5101 gcgtgcccgc catcggggtg acgaggacgt cgctgtcggc gatgcgaagc gtcgcatgat
     5161 cgctcagccg gggctgggcc ctgtcggtcg cggtgaagcg caggctggtc gtgccgaaca
     5221 gcgcgtccgg cgcctgcagg cggagcgaga agccgccgtc cgtgtcggcg tcgagcgtgg
     5281 tcagctgctg tccgctgagg ctcagcgcga cctctccgtc cggcgtgaat cccgacccgg
     5341 tgagcagcac gctctcgccg ctgctgtagc agggatatgt agtgcggagg gagggcggat
     5401 cggccgcgag cgccgcggct ccggggaagg cggcgacgag ggctgaggcg gcaagggcgg
     5461 cggcgctgcg ggacctcacg gcggacctcc gggagtcggt gggccggcgc ctcgaaaccg
     5521 accatacccg atcggcggag gttggtccag cacgggaacc atgcgtttta cagacccctc
     5581 ttgtacggac cgcgaccggc ctgatcgact ccgttcacga acgcgtcggc tagatccgcg
     5641 ggtgatcaaa ttgaccgcta taaagcggaa aatcgcgcgc cgactgacgg ccgtgagccc
     5701 tcccggttgt gccttgacat ccatagaacg atcgatctat ggtgcttcca cacgcggtct
     5761 ccagatcgcg ccgaacacga caggctgcac cagggagaga ggaaccaccg cgtgagatca
     5821 ggcttgggag acgaactgcg gatgcgcaac ggcgatcgtg gggcgggagc gtcgatgctc
     5881 ggcgtgccgg tgcgcacgct cgcgctgggc ctggccctcg ccatcgtggc gtctctcttc
     5941 gccgcggtcg tcgcgttcgg gtccaagggc ggggactcgt tcgtcggagt cccgaccgcg
     6001 tcgagcggcg aggccgccga cggcggcctc ttcaacaacc cgcgcgacat cgccgtcaac
     6061 cagaccggcg ccggcggggt ggacccgggc acggtgtacg tcgtcgacca gtccaaccac
     6121 cgcatccaac gcctcgacgc cgacggcaac ttcgagctga cctggggccg cgacgtcgtc
     6181 accgcgggcg ccgccaacga cgtgggcacg accgccttcg aggtctgcga cgtcacgcat
     6241 ccgacgaccc cgaacacggc ggcgaactgc aagggcggga ccactggctc gctgggtggt
     6301 ttgttgaaca acccggccgg cgtcgccgtc gaccaggaca ccggcgacgt cttcgtcacc
     6361 gaccgcggca acttccgcgt ccagcagttc gaggccgacg ggacgttcgt tcgtgcgtgg
     6421 ggctgggacg tcatcgcgaa cggcagcccg agcgacgcga ccaacgtgac gacgttccag
     6481 gtctgcacgg tcgctgcgga ttgcaaggcc ggtacgacgg gggcagggga ggggcagttt
     6541 ggatcgggga cgtccaactg gaggctcgac gtcagtcctg gcgacggcga ccccgcgagc
     6601 ggcaaggttg tcgtcgctga cccggggtcg ggatcgaccg gaaaccgtcg gattcagatc
     6661 ttcgagctcg ctgatcttgc gacaggcgct gcgccgaccg tggacgatgg ggagatcgtc
     6721 ggcggtgggg ccgccagccc attcttcggc ggcaacccgc agaacgtcgc cgtcgatgct
     6781 gctggcatcg tctacggaat cgatgttggc gacgtgactg tcaagcgcta cgacacagcc
     6841 tcaggatcgt ttctggcgcc gatttcgggg cagcggttca atgagttgta tccaggggat
     6901 ctcgccggcg ctttcgcggt tcccgagacg ctggaggtcg acccggagac gggcaatctc
     6961 ttcgtgaccg tcaatccgaa tattggcgcc gcagcgactg tcatatacga gtttgctgtg
     7021 ccagggggcg ggtccccgag catcgtggac ttccacatgc gcgacgccgg cgaccagacg
     7081 acaaacggcc tcggcattct ccattcggga gcgctggatc ggctgtatgt ctcggcttca
     7141 gtagggacga tcgggcaacg aatcctgatt ctcgacgaga acggtgccga gccgccaccg
     7201 tcggcgacga tcgctctgcc gactggcgtc ggcgcgcatg aggcaacgct caatggcacg
     7261 gtgaatccga acggaccgac cggcttctcg acgaagtacc acttcgagta cagcaaggac
     7321 ggatcgacat ggacggcagc cgccgcaccg gtggatctgg gtgacggatc ggccgacatc
     7381 gtcgtcgacg acagcgtgac cgggctcgag gcgaacacgc tctaccgcgc tcgcctggtc
     7441 acgacccgga ccttcgacgc cggcacgacc atctccgctg agacgacgtt caccacgccg
     7501 gcggtggcgc ccgaggtcac gaccggtcca gtccaacgca ggaccgcgac gaccgtcgag
     7561 ttgacgggcc ggatcaatcc gaacaacctt cccaccagct accacttcga gtacgggccg
     7621 tccgccgcgt acgggttcaa ggcgccgatt cccgatggcg acgccggctc ggcggggacg
     7681 gaggcgccgt tcgtggaggc catcgagggg ctgcaggcag gcgcttccta ccactaccgg
     7741 ctcgtcgcga cgaacgcgga ggggaccgcc tttggcgacg accgcacatt cagcactcgc
     7801 gcgagcgcca ccccgcctgc gggccgggcc tatgagatgg tcacccctgc ggacaagacg
     7861 aacaggcgca caggtgagtt cgcagcgacc gagcagggcc tcgtgaatcc ggggacgccg
     7921 tccaaggacg gcgagtcgct cctcttccgg cttcgctacg ggatcctcga tccagaggcc
     7981 ggaaccgact ttccgcattc cgtagatacg acgatcgtca ggcgaggctc agacgggtgg
     8041 gccggcgagt cggtaaccaa cgtcgtctcg ccacgcggcg cgacgggaag cctcgagtcg
     8101 ctccaggccg tgtccgcgga cctcgacacg ctggcctggc accacgccgc cgagctgttc
     8161 cagtccggct cgtcgttcag cacgaagctg atgggagaca ccggcggcct cgatgggtct
     8221 ggctggtacg agtgggtgat ggccccgccg ctcggggcca cgatcgactc gttcaccgac
     8281 gacgcggcgc tgatcgacga cgagggcgct cggatggtga ggtggggcca gtaccgcggt
     8341 ctgctcggtc ccgatgaccc gagccatccc gactacccga atcctgagaa cccgggccac
     8401 tccgcagacg cgttcacgca gacttcggga agcgcgatct atcgccagga gccagtgggc
     8461 tccgggcctc gccatctcgt caacgagtgc accggcaccg gatcgaccgc gacgctgatc
     8521 cccagccgca atgacaacgg aacggtgccc gggcccgcga gcgctctcgg caacgtgacc
     8581 ttcacggccg gctctgccga ggtgaccgtc aactcgatgt tggtggggac gtttggcgtc
     8641 ggtcatcgcg tggttgcggt cgaccccgcg cttcctgccg gaacggtgcc tgcgaacacc
     8701 ttcatcaccg cggtgggagc cgggacgttc acgatgtccg ctgccgccgg caacagcgcg
     8761 accggcgtcg ttcagggcca gcagaacagc gccgccacga acgacgactt catcgacgcg
     8821 cagccatgcg aggcaggatc ggtgaccagc ccgcgcggtg cggcggtcgg tgccggcggt
     8881 gcggatagcg gctcgccttc catctatggg cctgtggcca ccgccatgtc tcgggatggg
     8941 agccgggtgt tcttcacgag tccggatccg cgtgtgacgg ctggcgttcc cgtcacctgc
     9001 gggacgggga gcttccggct caccgactgt ccgccacagc tgttcgttcg ccagtacgac
     9061 gccgacggcg acgcgaccgt tcgctggata tcgcgctcgg aggtggacgg ccaagcgatc
     9121 gggctgatgg gcgccggggc gatcttccag ggagcttcgg ctgacgggcg gtacgtctac
     9181 ttcaagacca acgcgccgct ggtcgaggac gatccgaacg ggggggccat tacacccggc
     9241 ggcgtcacga ccggcacggc gtcaccgagc tcatgggaca tctaccgcta cgacctcacg
     9301 gcgaatacca gtgcggatcc cgcggacggt gcgctgatgc gcatctccgg tggcccctcc
     9361 ggcaccgcgg accccaacac gaaccccgtt ccctcggatt cgaccgtcgg aagcggaagc
     9421 tcggtccgat acgtatccga cgacggggcg agggtctact tcgtcaccgg cgcccccatc
     9481 ggtgccgccg atgacccctg gaacgagggg ccctcaggca ccgagcctgg aaccgcctcg
     9541 agcacccgga acctctacgt cttcgacgcc acggagtcag gcgaggcgcg gtggcggttc
     9601 atcgcgcaga ttccgtactc cgtcgaggga ggggtgctct ccgacgttgc gaactgtgcc
     9661 ggctccggct cggagtccgg gtacgcgaac gtgttctcga gcacgagcaa cgtgaatcac
     9721 cagcgggagg cggtgaactg cgtgcgcgga acccgagacg gctctgccat agcgttcgag
     9781 acccctgggc ggttgaccga ggacgacacg gactccgcct cggacatcta cctctacgac
     9841 gagcggtcgc atgagctgac gcggatcacg acgccaccgc ttggatcggc gccttatgcc
     9901 tgtgaccacg acccactcga ccgacccgtg gcgttttgca acggtgactt cggcttcccc
     9961 agtccgcccg gcgcccccag tcgtccagca ggtcaggagc gggtcggtct ggacggtcgc
    10021 caccatgaca acctcgcgat cgacgagtcc ggagagcttt cggtctactt cgagagccgc
    10081 gtgccgctgg tgcctgagga caccaatggc aatcacatgg acacctacga gtggcgcgcc
    10141 ggcgagctgt cgctgatctc gcccggcaag acgagcgacg acgcctggta ctccggcaac
    10201 agcgccgacg gtcaggacgt gttcttcttc acctcccagc gaatcgaccc acgtgagatc
    10261 gacgacggcg atctcgatgt ctatgacgcg cgggtggggg gcggcttccc gccgccgccg
    10321 ccgcggccgc agggctgcga ctcgcttgcc gatggttgcc agggccctgg tggtggaccg
    10381 ccgctggccg gggatcctca gacggatcga cccggcgggg ggaacgcgac gcccgatcgg
    10441 cgcgcgcgcc tgcgggcggt tcgcccgggt cgcagggcgc ttcgccgcgc cgcgcgaacc
    10501 ggcgtgatcc ggcttcgcgt gcggacgaac accgcgggac gcgtgtccgc gacggcgcgc
    10561 gcccgggtga aggtggggcg gaggtcgcgg acgatcgtcg tcgcgcgtcg ctcgatccgc
    10621 gtcggcaagc cgggggcacg tgtgctccgg ctgcgactga agaagtcggc gcggcgtcat
    10681 cttcggacgg gccggccgct tcgggtgagg ctgcgggtag cgcaggccga cgccgagccg
    10741 cgaagagtga aggtcgttct gaggagggcg ggccggtgac ggccggtaca cgcgcgaccg
    10801 cttatcggac tggatcacga gccatgcgaa ctgaggatcg attcatgacg tcggccattg
    10861 cagtacccat gccgcgcccc cgcgtcctgc tgcgccgact tgccctggtc ggggtcgtcg
    10921 cgacaatcgc gggtctgggt tcagcggtcc tcgcccacga tgcgagcgcg gatcacactc
    10981 atggggtggg cttcggcatc gccgacgcag tcggcgcggc ctggccgggc acgagctcgg
    11041 cggcatggtg ggcaggcacc tgcgacctcg acgaggcgag cacgtcgggg gtcggcgtcg
    11101 gtacgccgcc ggcgagcttc gcgcactgca tcgaccacgg cacgccgtac gacccggtcg
    11161 gcgagacgcc gacgccgccg cgcgagacga cctggcagcc gggcggcgag ccgtcgtggc
    11221 gattggaccc ggtcgctcag gccggcgcgc acccggatgc gacggcgtcg ttctggctcc
    11281 agcggtcccc gcatccggcg ctgtacggct cggctccggt cacgcttcag ggcgagggcg
    11341 aggcgcggac ggtgaaggtc tcgttgccgc cgggggtcgt cggcgatccg aacgccgttc
    11401 cgcgctgtcc gtccaaggcg atgcagacga cgcccacgac gtgtccgccg gagacccagg
    11461 tcggcgtcgc caccgtcact ctcgtgcaga cgggcacgcc gccgtacatg cagtccctgt
    11521 acccggtcta caacgcggag ccgcgcgacg ggaagacggc tgagctcatc gtctccgtcg
    11581 cgacgaacaa cgcgaaggcg aacatcccga tcgtcgctcg tgcgcgtacc gagggcgatt
    11641 tcggcgtgga cgggctcgcc gtcgaccttc cgggcgggat cccggtgctc ggccagacga
    11701 tcacgctgtg gggggttccc tgggcctcgt cgcatgacaa gttccgcccg cgcgcggggt
    11761 atgcgggcca ccagtacgct ggggggtctg ccggcggcat gcccgagacg ggcctgacca
    11821 acagcgatcc gacgcaggag ccgcagtcct acgatccttc ttggggcccg atacgcccgt
    11881 tcttcaccaa cccgaccgag tgcgcaccgg cgccgccggt gaccgagctc gggatcgcct
    11941 cgtggcagga ccccgatcga atgttgacct accccgcgtt cgccgacgcg ctcgtcgcgc
    12001 gatgcggcgc cgtgccgttc gacgccgaca tcgacgtccg gccgaccacc acggccgcag
    12061 acgcgccgac cggtatcgag gcggacctgc ggatcccgca gaacaacgac ccgccggccg
    12121 ccgtcgcgac gaatcccgac gacgcgaccg gagcgcccgc gcactgggag tccgacgccg
    12181 ggctcggcac ggcgcaactc gatcgcgccg tcgtgacgct tccgcgcggc gtctcggtca
    12241 atccctccgg ggccgccggc ctcgagggct gcagcgacgc ccagatcggc ctcacccgga
    12301 acggcaatcc gccgctgttc aacaacgagg acccgttcga cggccggggc gccgagtgcc
    12361 ccgacggatc gaagatcggc acggttgacg tctacacgcc gctgctgccg gcggcctacg
    12421 gcgaggtgcc cggcacgccg aacctgcgtg gcgaggtcgt gctcggcatg ccgcgctcga
    12481 ccgatccgac gtcaggcgac atgttccgca tgttcgtcgt cgtccgcaac gtgaagcgcg
    12541 gcctggtcgc caaggtgttc ggctcggccg tcgccgatcc ggctaccggg cagttgaccg
    12601 ccacgttcga tcgcaacccc cgtgtcccgt tcgagacgat gcagcttagg ttcaagggcg
    12661 gcgaccgcgg cctggtcgcg accccgcagc gctgcgccac ccatggttgg gcgacgaccc
    12721 tcacgccctg gaccgccgcc cacggcggcg gtggggcctc ggtcgccgac ggcggagcct
    12781 tcacgacgaa cgcgaactgc gggtacggct tctccccggc gctcgctgcc ggaatgagcg
    12841 agcggcgcgg ccgcgccaac gggacgttct cgctgacggt ggcccgggcg gacggtcagc
    12901 agtggttggc gggcctgtcg gcggagatgc cgaccggcct gctcgccaac gtcggcgatg
    12961 tgccgctctg cggcgacgcc caggccaacg ccggcgcctg cccgatcgcc tcgcggatcg
    13021 ggaccgtcga tgcggccgcg ggggccggca gtccgttcgt gctggagcgc aagggcgacg
    13081 tgtatttgac cgagggctac aagggcgccc cctacggcct cgcgacggtc gtccccgtgg
    13141 aggcggggcc gttccgcggg cggttcgccc tcacgccggt cgtcgtgcgg caggcgcttc
    13201 acgtcgatcg gaccgacgcg agcgtgaccg cggtctcgga tccgttcccg cagatctggc
    13261 atgggatacc gctgcgcgtg cggcgggtga cgatcagcat cgatcggccg ggcttcatgc
    13321 tcaacccgtc cgactgctcg ccgaaggcgg tcgccgcgac gatcgcctcg agcgaggggg
    13381 cggccgcgac gccggcggtg cccttccagg cgattggctg cggttcgctg cggttcaagc
    13441 cgaggctcgg gatgcggctg accggcaagc gtcagcgggt gacgggccgc catcctggcg
    13501 tgcgcgcagt ggtgcggcag cgggccgggg aggccgggat cgagcgggcg gtcgtgcggc
    13561 tgccgaagtc gcttgcgctc gaccccgaca acgcacgggc gctgtgtgag ttcgaggacg
    13621 gcaccaagcc cgacctcgag cgccactgcc ccaagggcag catcgtcggg cgggcgcgag
    13681 cgatcagccc gctgttgagg cggccgctgg tcggcaacgt gtacttcgtc aagaacgtcc
    13741 ggatcgacag ggacaccggc aacgagatcc gcacgctgcc gatgatcgtc gtcgcgctca
    13801 ggggcgagat cgcggtcaac ctgaggggcg agtcgagcgt caggggcaag cggctggtga
    13861 acaccttcgc cggcgtgccc gacgcgccgg tgtcgcggtt caacctcaac atccgaggcg
    13921 gccgcaacgg catcctcgcc gtcaccagaa cgcgcaaggc gaggatcaac ctgtgctcgg
    13981 cgcgtcggca gatagccgag gccgacatgg acggccacaa cggccggcgt cacgaccgca
    14041 acgtccggat caaggtcccc tgtcccaagc gccgcgccac cggcaagcgg cggcgctgac
    14101 ccactcttct gcccacgccc cggatcgaag gccgatgcct tcgctccggg ttcgtggcgg
    14161 aagtccccgc gccacgtcgg cgcggtaacc caaaccgctg accctgattg gtcagggtca
    14221 gtgacgtgat acccgaacaa ggagaaacca catgcgcaag ttcatgcttg tcgcagccgc
    14281 ggcactcgcc tcgatggcga cactcaccgc gaccgcgacc gcacaaacgc ccgtcgaggt
    14341 ccacgacccc agtaccggcg agcagtgcta ctacgtcgcg gtgatcgcag gtcaggtgtc
    14401 tggtggctgc gagtctcgca aggtcgcgaa cgaccttgtc gacctcggct acgagcttcc
    14461 tccctcgtgg ggcggaggtt ggatgcacac cgacagcggc ggcctcgcgt tcgacgtcaa
    14521 cgttggcagc tacggcgaca tctacatcag caacatcgcc ttcaccggct gggcgagcgc
    14581 cgcgagcgcg tgccgaggct ccgagccctg gaccggtgag atcatgcgca ccccggccgg
    14641 cgacctcgag gccgacgtcg acatctgcta cagccacatg ttcggcacgg cggacggcaa
    14701 cgtcacattc cggctcgacg acggcgatgc atggactgcg ctgattgccg accgccaggt
    14761 cgtcggcggt gggacccatg gcccgacctt cgagggccac ttcgtaggcg gcagtccact
    14821 ccaggtcatc gacgctcagt aggcaagcca tcgctcagcc gtcaggaaga cgacgagcac
    14881 gaccgcgggg gggggg
//
LOCUS       DATABV010000170         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1177739, whole genome shotgun sequence.
ACCESSION   DATABV010000170 DATABV010000000
VERSION     DATABV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177739"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VHF88_07410"
     CDS             complement(<1..634)
                     /locus_tag="VHF88_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX2161632.1"
                     /translation="MGIFSNLTGMGGRDMAVDLGTANTLVYVRGRGIVLSEPSVVAID
                     SRTAEVHAVGIEAKRMLGRTPGTIQAIRPLKDGVIADFDVTEQMLRHFIQKVHQNRWA
                     HPRVVVCVPSGVTGVEKRAVEEACLSAGARQAYLIEEPMAAAIGAGLPVGEPTGNMVV
                     DIGGGTSEVAVISLGGIVVSRSVRVGGDKMDEAIIGHMRRHYNMLIGEPTA"
     gene            complement(666..1223)
                     /locus_tag="VHF88_07415"
     CDS             complement(666..1223)
                     /locus_tag="VHF88_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX2161633.1"
                     /translation="MSAPRLVLASRSPQRRAILEQIGVDFEVVVPEVEELGEGEPGAV
                     VVENALRKARAAAGQLVLGCDTEVVSDGRVYGKPRDEREAERFLRELSGREHQVVSGL
                     ALIEAGRERTAASWTRVTFRELDEPTLRWYLASGEWRERAGGYAIQGRGAALVAAVAG
                     DYLNVVGLPLATLLELKPSLPLLHR"
     gene            complement(1233..1637)
                     /gene="ndk"
                     /locus_tag="VHF88_07420"
     CDS             complement(1233..1637)
                     /gene="ndk"
                     /locus_tag="VHF88_07420"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX2161634.1"
                     /translation="MDRTLILVKPDAFERSLTGEVIARFERKGLRIAALKRMEVSREI
                     AEQHYAEHSEKPFFGELVDFITGGPLVALVLEGHEAVKAARQLIGATNPIEAAPGSIR
                     GDFGLEVQTNLVHGSDSPESAAREIGIFFPEL"
     gene            complement(1659..2351)
                     /locus_tag="VHF88_07425"
     CDS             complement(1659..2351)
                     /locus_tag="VHF88_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2161635.1"
                     /translation="MPLLAIFGITNDGLNLVVNLLLLFLVVLWIALIFWTHADAKRRI
                     ADPMLVGCATVAAFFPFVGTIVYMVVRPPEYLEDVHERELEIAAAEARLAAAQEHACP
                     YCEHPVERAFLRCPSCLRRLKEPCGTCGKPLDPRWQICPYCEAELPERAPQQRRRGSG
                     RGRTASPPEGDPRARAKKRPAGTADQRPATSAGGRAAEPRRDRQPAPADPRTPEHPAA
                     RAQPERPEPRRQ"
     gene            complement(2639..>2979)
                     /locus_tag="VHF88_07430"
     CDS             complement(2639..>2979)
                     /locus_tag="VHF88_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161636.1"
                     /translation="LPEVIADRHPLVLVISVLEDKAAADMLRPLLPLADHLVLTRCAN
                     PRALSPATLESLADQLDGPPAEIVADPLAALERARALAGPQGAVLATGSIYLIADLVR
                     DQGETHASAL"
BASE COUNT          438 a         1016 c         1092 g          433 t
ORIGIN      
        1 cggcggtcgg ctcgccgatc agcatgttgt agtggcggcg catgtgcccg atgatcgcct
       61 catccatctt gtcgccgccg acgcggacgc tccggctgac gacgatgccg ccgagcgaga
      121 tgacggcgac ctcgctcgtg ccgccgccga tgtcgacgac catgttgccg gtcggctcgc
      181 cgaccggcag gccggccccg atcgccgcgg ccatcggctc ctcgatcaga taggcctgcc
      241 gcgcgccggc ggacaggcac gcctcctcga ccgcgcgctt ctccaccccg gtgacccccg
      301 acggcacgca gaccaccacg cgcgggtgcg cccagcggtt ctggtgcacc ttctggatga
      361 agtggcgcag catctgctcg gtgacgtcga agtcggcgat cacgccgtcc ttgagtgggc
      421 ggatcgcctg gatcgtcccc ggcgtgcggc cgagcatccg cttcgcctcg atcccgaccg
      481 cgtgcacctc cgccgtgcgc gagtcgattg ccaccaccga cggctcggag agcacgatcc
      541 cgcgcccgcg cacgtacacg agcgtattgg cggtgccgag atcgacggcc atgtcgcggc
      601 cgcccattcc cgtgaggttg gagaagatgc ccatcggagg aaggaccaga atacgacctg
      661 cgccgctagc ggtggagaag cggcaaggaa ggcttcaact caagcagggt agccagtggc
      721 agcccgacca cgttgaggta gtcgccggcg accgcggcga ccagcgccgc gccgcgcccc
      781 tggatcgcat agccgcccgc ccgctcgcgc cactcgccgc tcgcgagata ccagcgcagc
      841 gtcggctcgt cgagctcgcg gaacgtgacg cgcgtccagc tggcggccgt ccgctcgcga
      901 cccgcctcga tcagagccag tcccgagacc acctggtgct cgcggccgga cagctcgcgc
      961 aggaaccgct cggcctcgcg ctcgtcgcgc ggcttcccat agacccgccc gtcggagacg
     1021 acctccgtat cgcagcctag gaccagctgg cccgcggcgg cgcgcgcctt gcgcagggcg
     1081 ttctcgacga cgaccgcgcc cggctcgccc tcccccagct cctcgacctc cggcacgacg
     1141 acctcgaagt cgacgccgat ctgctcgagg atcgcccgcc gctgcggcga gcgcgaggcc
     1201 aggacgagcc gaggcgcgct caccgcgccg ggctacaact cggggaagaa gatgccgatc
     1261 tcgcgcgccg ccgactcggg cgagtcggac ccgtggacga ggttcgtctg gacctcgagg
     1321 ccgaagtcgc cgcgaatcga gcccggcgcc gcctcgatcg ggttcgtcgc gccaatcagc
     1381 tggcgcgcag ccttgaccgc ctcatgcccc tccagcacca gcgcgaccaa cgggccgccg
     1441 gtgatgaagt cgacgagctc gccgaagaac ggcttctcgc tgtgctccgc gtagtgctgc
     1501 tcggcgatct cgcgcgagac ctccatccgc ttcaacgcag cgatccgcag ccccttgcgc
     1561 tcgaagcggg cgatcacctc gccggtcagg ctgcgctcga acgcgtcggg cttcaccagg
     1621 atcagggtcc tgtccacgtc ttctccttcg tcggggggtt actggcggcg cggctcgggt
     1681 cgctcaggct gggcccgagc ggccgggtgc tccggggtgc gcgggtcggc aggcgccggc
     1741 tggcgatcgc gacggggctc ggctgctctg ccgccggcgc tcgtcgcagg tcgctgatcg
     1801 gccgtgccgg caggccgctt cttggcccgg gcgcgcgggt ctccctcggg cggcgacgcg
     1861 gtgcggccac gacccgagcc gcgacgacgc tgctgcggcg cgcgctcggg gagctcggcc
     1921 tcgcagtaag gacagatctg ccagcgcggg tcgagcggct tgccgcaggt gccgcagggc
     1981 tccttgaggc gccgcaggca gctcggacag cgcagaaacg cccgctcgac cggatgctcg
     2041 caatacgggc aggcgtgctc ctgagcggcg gcgaggcgag cctcggcggc ggcaatctcg
     2101 agctcgcgct cgtgcacgtc ctcgaggtac tccggcgggc gcacgaccat gtagacgatc
     2161 gtcccgacga atgggaagaa ggccgcgacc gtcgcgcagc cgacgagcat cgggtcggcg
     2221 atccggcgct tggcgtcggc gtgcgtccag aagatcagcg cgatccagag gaccacgagg
     2281 aagagcagca gcaggttgac gaccaggttc aagccgtcgt tagtgatgcc gaagatggcg
     2341 agcaggggca tggggcgggc agcctagagg aagacccggc cgattccgta gccgatggcg
     2401 aagaagagga ggatgacgga ggctacgacg atggcgacga gggcgatcat tgcgccaaag
     2461 ctgggtccgt cgccgcggtc catgcggggg attgtcccat gctgcggttg tggcggctct
     2521 ggttagggat cggcgttgca ggccgcgggt cctgtctccg attttggttt gggtgctcgc
     2581 aagctgcgcg cccaaacgca aaatcgtcgc cacccgcggg gtgcgacgtc tactgcggct
     2641 aaagcgcgga tgcgtgtgtc tctccttggt cgcggactag gtctgcgatg aggtagatgg
     2701 agccggtggc aaggacggcg ccttgggggc ccgcgagggc gcgggccctt tcgagggcgg
     2761 cgagtgggtc ggcgacgatc tccgctggag ggccgtcgag ttggtcggcg agggattcga
     2821 gggtggccgg tgagagggcg cggggattgg cgcagcgggt gaggacgagg tggtcggcga
     2881 gtgggaggag tggccggagc atgtcggcgg ctgccttgtc ctcgaggacg ctgatcacga
     2941 ggacgagcgg gtggcgatcg gcgatgacct cgggcaggg
//
LOCUS       DATABV010000171         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1018379, whole genome shotgun sequence.
ACCESSION   DATABV010000171 DATABV010000000
VERSION     DATABV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018379"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..471
                     /gene="rplM"
                     /locus_tag="VHF88_07435"
     CDS             37..471
                     /gene="rplM"
                     /locus_tag="VHF88_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX2161637.1"
                     /translation="MKTFVATPENRERDWLVVDATGQTLGRLATQIAIALRGKHKPEY
                     TPHCDVGDFVVVVNADKIVVTGRKREEKLYHRYSGYPGGLRSRTFAEMLDRRPEEPLR
                     LAVKGMLPRNRIARAQLRNLKIYAGPDHPHAAQQPKPMEIEN"
     gene            936..1331
                     /gene="rpsI"
                     /locus_tag="VHF88_07440"
     CDS             936..1331
                     /gene="rpsI"
                     /locus_tag="VHF88_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX2161638.1"
                     /translation="MAADARYRATGKRKSAIARVTLKPGSGVYTINGKTLDDYFPRAA
                     LQTKARRPLEIVGQQDRMDVVAKIQGGGVSAQAGALRHGITKALVEADPNLRGELKRR
                     GFLTRDPRVKERKKAGLKKARKRPQFSKR"
     gene            1341..2663
                     /gene="glmM"
                     /locus_tag="VHF88_07445"
     CDS             1341..2663
                     /gene="glmM"
                     /locus_tag="VHF88_07445"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX2161639.1"
                     /translation="MGELFGTDGVRGAAGEFLTADLALALGRAATVTAGESAAAAPQV
                     LIVRDTRESGEMLESALAAGVAAAGGHALLGGVLPTPGAALLTRRHGFDLAAVVSASH
                     NPYADNGIKFFGPDGTKVPDDREAAIEALVAGPEHSSASIGRIRTLHGAAGDYLRELE
                     LRFGDLDLDGRRVLLDCANGATYEVAPQIFRRLGATVDALAVEPDGRNINDGVGSTHV
                     EAISQRMREDGYDVGFAFDGDGDRVLAVDRRGEVVDGDELVALAALHLRRSGRLRGDG
                     VAVTVMTNYGFHTAMEEAGIEVAVTPVGDRHVLAELLRRGWGLGGEQSGHIIETGFVP
                     SGDGIAAALLTLEALGDGDLSERRGMRKLPQRLVNVRVRDRGALDRATAVWGAVETES
                     SALEGRGRVLVRPSGTEPLVRVMVEAPDEGECAAVTDRLVAVVQRELA"
     gene            2694..4526
                     /gene="glmS"
                     /locus_tag="VHF88_07450"
     CDS             2694..4526
                     /gene="glmS"
                     /locus_tag="VHF88_07450"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX2161640.1"
                     /translation="MCGIVGYTGGRPCREILLAGLEKLEYRGYDSAGISLLEDGEIRT
                     VRAVGNLSRLRDAVGDNGFGAGALAGIGHTRWATHGRVTEANTHPHDDSSGRIHVVLN
                     GIVENWSELRQQLEGDGAAFSSETDAEAVAHLIAARYEGDLVAAVRAAYGELRGHYAF
                     VAVHADEPDTLVAARKECPLVVGVGEGESFVASAIPAFLRDTRRVQLVENDEIVVITP
                     QGARFTTAAGDEVERPAEEVDWDEEAAEKGGYETFMLKEIHEQPDAVAETVADRLIGD
                     GVQLGEIGISDDELRDLRRIVVVACGTSYHAGLSGRYAIEAWSRVPVEMDVASEFRYR
                     DPVVDERDLVIGITQSGETADTLAAMRLAREKGARVLAVTNVMGSQATRDADGVLFTR
                     AGLEIGVAATKTYVSQVAAMLLLALRIAEVRATLDRPRIAELVDELRALPRRIDDLLE
                     QVDAPVRQMADRWRDAGFFLYLGRHAGLATALEGALKLKEISYVPTDAYAAGEMKHGP
                     IALLDERTPVVCVATDSPVLDKVLSNIAEVRARGAHVLAVATRGNDEVGRHAEEVVYV
                     PDTDWLIQPLLAVIPLQLLAYYIARGRGLNVDQPRNLAKTVTVE"
     gene            4602..>4895
                     /locus_tag="VHF88_07455"
     CDS             4602..>4895
                     /locus_tag="VHF88_07455"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX2161641.1"
                     /translation="MIGIDLLEIARLERAVQRRPRLADRLFTPAERQYAAGKARPAQH
                     LAARFCAKEAVAKALALNAWAFSDIEVVATDAAPRVRLGGSAAARASELGADVA"
BASE COUNT          741 a         1771 c         1759 g          624 t
ORIGIN      
        1 cgagcccccg gtccttatgc cacactgtgc cgctgcatga agacctttgt cgccactccc
       61 gagaaccgtg agcgcgactg gctcgtcgtc gacgcgaccg gccagacgct cggccggctc
      121 gcgacgcaga tcgcgatcgc gctgcgtggc aagcacaagc cggagtacac gccgcactgc
      181 gacgtcggcg acttcgtcgt cgtggtcaac gccgacaaga tcgtcgtcac cggccgcaag
      241 cgcgaggaaa agctctacca ccgctactcc ggctacccgg gtggcctgcg cagccggacg
      301 ttcgccgaga tgctcgaccg gcgtcccgag gagccgctgc ggctcgccgt caaggggatg
      361 ctgccgcgca accgcatcgc tcgcgcgcag ctgcgcaacc tgaagatcta cgccggcccc
      421 gaccacccgc acgccgcgca gcagcccaag cccatggaga tcgagaactg atggccgacg
      481 agcccaagga caccccgcag gacgagccgg ctcccgagca gccgaccgcg gagcagcccg
      541 cagccgagga gcccgaggcg acgaccgact ctcccgccgc cgaggagccc gaggcgacgg
      601 ccgacgctcc tgcggaggac gaggccgagc cgactgacga gactcctgca gcggaggagg
      661 ccgccgctga gcccgaggcg agcgagaagc cggccgagaa gccgaagccg aagcgcaaga
      721 aggacgccaa ggccgacgag cccgaggtca ttcccggcgc ccacctcgag cccgacctgg
      781 tgctcgagga ggagcagcag cgtcccgacg agccgagcta cgtgggggcc gaggtcgagg
      841 tcgccgccga cgacgctccg gtcgagggcg aggagccggc tgccgacgat gccgacgagg
      901 acgaggcacc tggtccgctc gaggcggtcc tcgacctggc cgccgacgcg cgctaccgcg
      961 cgactggcaa gcgcaagagc gcgatcgccc gcgtgacgct gaagcccggc agcggcgtct
     1021 acacgatcaa cggtaagacg ctagacgact acttcccgcg cgccgcgctt cagacgaagg
     1081 cgcgtcgccc actcgagatc gtcgggcagc aggaccgcat ggacgtcgtc gcgaagatcc
     1141 agggcggggg cgtatcggcc caagccggcg cgctgcgcca cgggatcacc aaggcgctcg
     1201 tcgaggccga cccgaacctg cgcggcgagc tgaagcgccg cggcttcctg acacgcgatc
     1261 cgcgtgtcaa ggagcgcaag aaggccggac tcaagaaggc gcgcaagcgc ccgcagttct
     1321 cgaagcgcta ggcaccagcc ttgggagagc tgttcggtac ggacggcgtc cgcggcgccg
     1381 ccggggagtt cctgaccgcc gacctggcgc tggccctcgg ccgcgcggcc acggtcacgg
     1441 cgggagagag cgccgccgcc gcgccgcagg tcctgatcgt ccgcgacacg cgcgagtccg
     1501 gcgagatgct ggagtcggcg ctcgcggcgg gcgtggccgc ggcgggcggc cacgcgctgc
     1561 tcggcggtgt gctgccgacg cccggcgcgg cgctgctcac ccgccgccac gggttcgacc
     1621 tcgcggcggt cgtgtcggcg tcgcacaacc cctacgcgga caacgggatc aagttcttcg
     1681 gccctgacgg caccaaggtt cccgacgatc gcgaggcggc gatcgaggcg ctcgtcgccg
     1741 gccccgagca ctcctccgcc tcgatcgggc gcatccgcac gctgcacggc gccgcaggcg
     1801 actacctgcg cgagctcgag ctgcgcttcg gcgacctcga cctcgacgga cgccgggtcc
     1861 tgctcgactg cgccaacggc gccacctacg aggtcgcgcc gcagatcttc cgccgcctcg
     1921 gcgcgaccgt cgacgcgctc gccgtagagc ccgacggccg caacatcaac gatggcgtcg
     1981 gctcgaccca cgtcgaggcg atttcgcagc ggatgcgcga ggacggctac gacgtcggct
     2041 tcgccttcga cggcgacggc gaccgcgtgc tcgcggtcga ccgccgcggc gaggtcgtcg
     2101 acggcgacga gctcgtcgcg ctcgcggcgc tccacctacg ccgttccggc cggctgcggg
     2161 gcgacggcgt cgccgtcacc gtgatgacca actacggctt ccacacggcg atggaggagg
     2221 ccggcatcga ggtcgccgtg acgccggtcg gcgaccgcca cgtgctcgcc gagctgctgc
     2281 gtcgcggctg gggtctcggc ggcgagcagt cgggccacat catcgagacc ggcttcgttc
     2341 cctcaggcga tggaatagcc gcggcgctgc tgacgctcga ggcgctcggc gatggcgacc
     2401 tcagcgagcg gcgggggatg cgcaagctgc cgcagcggct ggtcaacgtg cgcgtgcgcg
     2461 accgcggtgc gctcgatcgg gcaaccgcag tttggggggc cgtcgagacg gagtcgagcg
     2521 cgctcgaggg ccgtgggcgg gtgctggtcc ggccgtccgg gaccgagccg ctcgtgcggg
     2581 tgatggtcga ggcgcccgat gagggcgagt gtgccgccgt gaccgaccgg ctggtggcgg
     2641 tcgtgcagcg cgagctggcc tgacgccgca gccggctacc ctctcggtcc tccatgtgcg
     2701 gcatcgtcgg atacacgggc gggcgcccct gcagggagat cctgctcgcg ggcctcgaga
     2761 agctcgagta ccgcggctac gactccgccg gcatctcact gctcgaggac ggcgagatcc
     2821 ggacggtgcg cgcggtcggc aacctgtcgc gacttcgcga cgccgtcggc gacaacggct
     2881 tcggggcagg cgcgctcgct gggatcgggc atacccgctg ggcgacgcac ggtcgcgtca
     2941 ccgaggccaa cacccatccg cacgacgaca gctcgggccg catccacgtc gtcctgaacg
     3001 ggatcgtcga gaactggtcc gagctgcgcc agcagctcga aggcgacggc gcggccttca
     3061 gctccgagac cgacgccgag gcggtcgcgc atctgatcgc cgcccgctac gagggagacc
     3121 tcgtcgccgc cgtccgcgcc gcctacggcg agctgcgcgg gcactacgcc ttcgtcgccg
     3181 tgcacgccga cgagcctgac acgctcgtcg cggcgcgcaa ggagtgcccg ctggtcgtcg
     3241 gcgtcggcga gggcgagagc ttcgtcgcct ccgcgattcc cgccttcctg cgcgacaccc
     3301 gccgcgtcca gctcgtcgag aacgacgaga tcgtcgtgat cacaccgcag ggggcgcgct
     3361 tcacgaccgc cgccggcgac gaggtcgagc gcccggccga ggaggtcgac tgggacgagg
     3421 aggccgccga gaagggcggc tacgagacgt tcatgctcaa ggagatccac gagcagccgg
     3481 acgcggtcgc tgagacggtc gccgaccggc tgatcggcga cggcgtccag ctcggcgaga
     3541 tcggcatctc cgacgacgag ctgcgcgatc tgcggcggat cgtcgtcgtc gcctgcggaa
     3601 cctcctacca cgccgggctc tcgggccgct acgcgatcga ggcctggtcg cgggtcccgg
     3661 tcgagatgga tgtcgcgtcg gagttccgct atcgcgaccc agtcgtcgac gagcgcgacc
     3721 tggtgatcgg cataacgcag tccggcgaga ccgccgacac gctcgcggcg atgcggctcg
     3781 cgcgcgagaa gggcgctcgc gtcctcgcgg tcaccaacgt gatgggcagc caggcgacgc
     3841 gcgacgccga cggcgtcctc ttcactcgag ctggtctgga gatcggcgtg gcggcgacca
     3901 agacctacgt ctcgcaggtc gcggcgatgc tgctgctggc gctgcggatc gccgaggtgc
     3961 gcgcgacgct cgatcggccg cggatcgccg agctggtcga cgagctgcgc gccctgccgc
     4021 ggcggatcga cgacctgctc gagcaggtcg acgccccggt ccgccagatg gccgaccgct
     4081 ggcgggacgc cggcttcttc ctctacctcg gccgccacgc cggcttggcg actgcgctcg
     4141 agggcgcgct gaagctgaag gagatctcct acgtgccgac cgatgcgtac gcggccggcg
     4201 agatgaagca cggtccgatt gccctgctcg acgagcgcac gccggtcgtc tgcgtggcga
     4261 ccgactcgcc ggtgctcgac aaggtgctgt cgaacatcgc cgaggtccgg gcgcgcgggg
     4321 cccacgtact ggccgtcgcg acgcgcggca acgacgaggt cggccgtcac gcagaggagg
     4381 tcgtctacgt gcccgacacc gattggctga tccagccgct gctggcggtg attccgctcc
     4441 agctgctcgc ctactacatc gcccgtggcc gggggctcaa cgtcgaccag ccgcgcaacc
     4501 tcgcaaagac cgtcacggtc gagtagcaaa gccatgccag agcctgcgcg aagcgttctt
     4561 aggccagtgg cccggaagaa ccttcgctcg ggcggtccaa gatgatcggc atcgaccttc
     4621 tcgagatcgc ccgcctggag cgcgcggtgc agcgtcgccc gcgactggcc gatcgcctct
     4681 tcacgccggc cgagcggcag tacgcggccg gcaaggctcg tccagcccag cacctcgcgg
     4741 cgcgcttctg cgccaaggag gccgtggcca aggcgctcgc cctgaacgcc tgggccttct
     4801 ccgacatcga ggtggtcgcc accgacgcgg caccgcgggt ccgccttggc ggctccgccg
     4861 ccgcgcgggc gtcggagctc ggtgcggacg tcgcg
//
LOCUS       DATABV010000172         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2865968, whole genome shotgun sequence.
ACCESSION   DATABV010000172 DATABV010000000
VERSION     DATABV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865968"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1514)
                     /locus_tag="VHF88_07460"
     CDS             complement(138..1514)
                     /locus_tag="VHF88_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX2161642.1"
                     /translation="MTEIDPGIFKAYDVRGLYGSQIDETTAYAVGRGFARVLAGMYDT
                     EPSALRLALGRDMRLTAPALAAEYARGMSDEGAAVLDVGMVGTEMLYWTVGSRELDGG
                     LMCTASHNPKAYTGAKLVGRGALALSGDAGIGELRELLTGGALGEPAGRVGAIEREDV
                     YEPFGRDALAWIDPNAVAPLKVVLDGGNGMAGPLCGPLLDQTAIAQVQLYWKPDGEFP
                     DHEPNPLLEENRRFIVDKVRSEGADLGIAWDGDADRCFFIDGSGAFVDGDFLTALLAK
                     SILEREPGATILYDVRASRAVRDVVEQAGGTAHVNRVGHAFFKTRMRDEGAAFGGEVS
                     GHYYFRDFYCADSGTLPALLILELLSKEGRTLAELLADLRGRYFISGEINSEVADQQA
                     KMDEIAARYSDGELSWLDGVSVDYDDWHFNVRPSNTEPLLRLNLESLVSPEHMAAKRD
                     EVLGLIRS"
     gene            complement(1526..>1946)
                     /locus_tag="VHF88_07465"
     CDS             complement(1526..>1946)
                     /locus_tag="VHF88_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX2161643.1"
                     /translation="SNVKQEIINFRKIIRPQRSTLRDLERTKQRYMAADLDVYFDDIQ
                     DASERIWDMLENYKEVVEALESTNESVISHRVNDILRVLTSISVMVLPLTLVASLWGM
                     NVGVPGEGSTAGFWVVLGAMVALLLGMLGYFRRRGWL"
BASE COUNT          296 a          675 c          678 g          297 t
ORIGIN      
        1 ggcgtcgacg agcgtcagcg gctcgtcgtc gatcagatag cagttgaccc gtccgacggc
       61 gaacggggtc gggatcgaga ggcggtgcac gccagcggat gccgcgacgg cgaagccgcc
      121 cggctcggtc gcggcggtca cgacctgatc agcccgagca cctcgtcgcg cttggcggcc
      181 atgtgctcgg gggagaccag cgactcgagg ttgagccgca gcaacggctc ggtgttcgac
      241 ggccggacgt tgaagtgcca gtcgtcgtag tcgaccgaca cgccgtcgag ccaggacagc
      301 tcgccgtcgg agtagcgcgc ggcgatctcg tccatcttcg cctgctggtc ggcgacctcg
      361 gagttgatct cgccggagat gaagtacctg ccgcgcaggt cggccagcag ctcggcgagc
      421 gtccggccct ccttcgacag cagctcgagg atcagcagcg ccggcagggt tccggagtcg
      481 gcgcagtaga agtcgcggaa gtagtagtga cccgagacct cgccgccgaa ggccgcgccc
      541 tcgtcgcgca tccgcgtctt gaagaacgcg tggccgacac ggttgacgtg cgcggtcccg
      601 ccggcctgct cgacgacatc gcgcaccgcg cggctggcgc gcacgtcgta gaggatcgtc
      661 gccccgggct cacgctcgag gatcgacttc gccagcagcg cggtcaggaa gtcgccgtcg
      721 acgaacgctc cgctgccgtc gatgaagaag cagcggtcgg cgtcgccgtc ccaggcgatg
      781 ccgaggtcgg cgccctcgga tcgcaccttg tcgacgatga agcggcggtt ctcctcgagc
      841 agggggttgg gctcgtggtc tgggaactcg ccgtcgggct tccaatagag ctggacctga
      901 gcgatcgcgg tctggtccag cagcggaccg cagagcggcc ccgccatgcc gttgccgccg
      961 tcgagcacga ccttcagcgg tgcgacggcg ttggggtcga tccaggcgag cgcgtcgcga
     1021 ccgaacggct cgtagacgtc ctcgcgctcg atcgcgccga ctcgaccggc cggctcgccg
     1081 agcgcgccac cggtcagcag ctcgcgcagc tcgccgatcc cggcgtcgcc ggagagcgcc
     1141 agcgctccgc gtccgaccag cttcgcgccg gtgtaggcct tcgggttgtg cgaggcggtg
     1201 cacatcaggc cgccgtcgag ctcgcgcgag ccgacggtcc agtagagcat ctcggtgccg
     1261 accatcccga cgtcgagcac cgctgcaccc tcgtcgctca tcccgcgcgc gtactcggcg
     1321 gccagcgcgg gcgccgtcag gcgcatgtcg cgtccgagcg cgagtcgcag cgcgctcggc
     1381 tcagtgtcgt acatccccgc caggacgcgc gcgaagccgc gcccgaccgc gtacgcggtc
     1441 gtctcgtcga tctgcgagcc gtagaggccg cggacgtcgt aggccttgaa gatccctggg
     1501 tctatctcgg tcacgcggcg agaggctaca gccagccccg ccgccggaag tagccgagca
     1561 tcccgagcag cagggcgacc atcgcgccca gcacgaccca gaacccggcg gtcgatccct
     1621 cgcccggcac gccgacgttc atcccccaga ggctcgccac caacgtcagc ggcagcacca
     1681 tgacgctgat cgacgtcagc acgcgcagga tgtcgttgac gcggtgcgag atcaccgact
     1741 cgttggtcga ctcgagcgcc tcgacgacct ctttgtagtt ctcgagcatg tcccagatcc
     1801 gctcggaggc gtcctggatg tcgtcgaagt agacgtcgag gtcggcggcc atgtagcgct
     1861 gcttggtccg ctcgaggtcg cgcagcgtcg agcgctgcgg ccggatgatc ttgcggaagt
     1921 tgatgatctc ctgcttgacg ttggag
//
LOCUS       DATABV010000173        26042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2304288, whole genome shotgun sequence.
ACCESSION   DATABV010000173 DATABV010000000
VERSION     DATABV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 26042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26042
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304288"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2255
                     /locus_tag="VHF88_07470"
     CDS             <1..2255
                     /locus_tag="VHF88_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2161644.1"
                     /translation="ERGEERPGLDPGRPRGGAQHHQRVERQAAERRRQRRVESQVVER
                     VRQRGEVRAQVRRLLLAPVAARADRVRRQPTLGQRALEDRGIPTYVMGGRGYWSQQQV
                     GDLRAYLAALANPLDDLALYTALVSPLGGVSLDALVVLRAAAQRARVEPWTLLAELAG
                     VSGGRVAVDGGSRGGRAQELAAQLGDRDAERARAFVRLLAEERAAAPRSSLEALIDRG
                     VTRSGYDLHLLALPAGRRRMANVRKLMRLAREFEAEEGRDLRRFIDYVAERDELGERQ
                     GEAPLEPEDVQAVRLMTVHRAKGLEFPVVCVADLGKPGREDRSALQISDDGAVGISLA
                     QLGGGSVTSSRLKEIRERQKLADEEEEKRVFYVAATRAEEHLILSGGTDLEKLSEEKP
                     LEEPMRWIWRALAPGLGEIDTSAVVEHELDGRPVAVRCTVLRPDTVDAVLPAADRTPS
                     APEQPPAGLDALDAPALAAVAPPTALPISRISYSALQRHRECGYRFYLERSLRLPRGE
                     ALPEPAEPEAEAVDALPPTVRGSIAHSLLEEIDFAQPVTPTVEEVAALIEAHREPVRD
                     DEVADILGLFESFAASRLRQRLAAAHSVRTELPFAFELAPDTAGGRTLLVNGIVDVHA
                     VESDRTLIVDYKSNRLEGATPTEIVESDYEIQRLVYALAALRTGAPRVEVAYVFLERA
                     ADPVSAVYEASALEDLDRELRDIAAPVAAGSFIPTPQPWRGLCADCPGQPGLCSWPPE
                     RTLAHSHSGG"
     gene            2572..4125
                     /locus_tag="VHF88_07475"
     CDS             2572..4125
                     /locus_tag="VHF88_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2161645.1"
                     /translation="MFELIASWSEDIGNLTGPLAIVALALVFSLYRNEIRGFLANASK
                     LRVQEPGGELEMTSQRDSDSEDAETSEPAESASSADPNEELNAPKTEVEGAAATDADY
                     ESETDTDKLRVRMGNALEAKDEEKAEAIFKRLQQVEENPVQGKVDQGLRLWLRFVFKF
                     EPDALVKLEELAADERVAAHMYLFRGYCLSRAGNTSGAASELKRARDAATSSDVKTRA
                     TIFYAKELDKAGDQAQSLREIETALRECEDPSLRASLWRALAELYEQHDQHELRALAL
                     QKALEAAPDNARLRLDVAYAYSHADESDFGPLVIHHYETALSFEPTYETARNNLGVAY
                     NHADLTILSAKALKRASDDGSGLATGNLAFRYLNAGFADQAEELVKEATKGGSPLHAK
                     VAEASADIAQRRESENEQRTRLSALGQRQAAFLSDYAEAQVKPATSLLGTWRFQDSGD
                     EVTVTGENDSLLGGVDCRREYQVEVRGNQPRADRASQGLRDAVLDLERKARGAQLLDP
                     GAGLRVPNR"
     gene            4851..5330
                     /locus_tag="VHF88_07480"
     CDS             4851..5330
                     /locus_tag="VHF88_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161646.1"
                     /translation="MDRETANVLIVDWRWEGPANDQPSTDASRRLTQLMLELVGQPEL
                     VSGVTLEDGSRAALVLHEGSLFITTATGEGSDVQVSVRREPLDSNRVLVTMNERKEGQ
                     PPVTWVRTWTFQRVGEEPFLSFLTHSHRRDEKEPRATAFARRLCRTVGWDIPVEEPR"
     gene            5363..6520
                     /locus_tag="VHF88_07485"
     CDS             5363..6520
                     /locus_tag="VHF88_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161647.1"
                     /translation="MNELSRYRDSLPPVWAVPELGLCQLTKPSPAIDSVLSSYYEDPE
                     HADAGGYIDALISHVLRSPRLSYDEVRDLADPVRAELRVACAVYAGVGLPPERLTDRR
                     LLVAMAERFRRQASQPSDGGFLGFFRSLQGLGEHAAAQLHHRLRGVPFEVAAQAVADA
                     FEDFRKDQERKLDEVRRQPLFFIVADLPVSDARWLLFEAPENIATLVRETVMTDPTFL
                     DNVERAVLDAPHLDEPQRDDLLKGLKAVRNGDPYACRHLLAGVEGGLWLAAEDEGVID
                     SNRLLLRSKKKNEPFARNFGRFLDTDDGGLDVGDDYSWFLRRAAYDEQGQAIRHGRAR
                     IGHADYSVWSFVALLGWLDRFSGTTFMYEVTDRFMMEPDETETEMVGQEPR"
     gene            complement(6777..7271)
                     /locus_tag="VHF88_07490"
     CDS             complement(6777..7271)
                     /locus_tag="VHF88_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161648.1"
                     /translation="MPPTASHDGAENAYELAYDEAVLAVSSQQAAIDSFRGRAGLLLS
                     GAAIATSFLGGQALRTGNPGVWSWISIAVFALLGLATLAILWPRNWKFEADPADIVAT
                     YIEAEEPLPVVDIQRDLALHRASVLEENGKQFRRLVWSFRAAGILLVVEIVTWTIDLA
                     TKVD"
     gene            7359..7814
                     /locus_tag="VHF88_07495"
     CDS             7359..7814
                     /locus_tag="VHF88_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161649.1"
                     /translation="MPLCFIEPQEAIRVIADAVGREARRQLLGEVVRAWVDEIPDDEL
                     AVHYAKAVEVLDDDDLSMLAAWHASLEVGQPIANKDFLLKAFGSLGEHRREALRIAAG
                     FRGDDAFEAGVSEEQAIDTVLPFLSHERMTELAAEAIELYCWDRMGSDN"
     gene            7970..8323
                     /locus_tag="VHF88_07500"
     CDS             7970..8323
                     /locus_tag="VHF88_07500"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2161650.1"
                     /translation="MARAATTADAFNAVAEPRRREILDLLAGGESAVNDIVERLGLTQ
                     PQVSKHLRVLREVGLVDVRDEGRQRLYRLNGPALKPIHDWVSSYERTWSERFERLDAV
                     LEDLKRKDEGDDLHD"
     gene            8316..8810
                     /locus_tag="VHF88_07505"
     CDS             8316..8810
                     /locus_tag="VHF88_07505"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2161651.1"
                     /translation="MTSSGKANVTLPTDEQILITREFDAPKELVYQAWTTPELVRRWW
                     SGNRGEMTVCEIDLRVGGKWRYAMVANGGQEVAFHGEFREIVPNERVVSTEAYEGIPD
                     ADEHATLNTLTFTDLGGGRTLMEVLVAHPTKLGRDMHIDSGMEIGLQEALDHLEEVAV
                     SLRS"
     gene            complement(8807..9439)
                     /locus_tag="VHF88_07510"
     CDS             complement(8807..9439)
                     /locus_tag="VHF88_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2161652.1"
                     /translation="MSAPKAVISDFGGVLTTPLGNAFAAWSKESGVALEDLGKALVAS
                     HERHGEHPLFLLERGELTEPEFMARLEQELGGQRLDGMGEAFMAGLERNHEMIELLAD
                     LRDRGLRTALLTNNVREWEARWRAMLPEIDEIFEVVVDSAFVGMRKPEPEIYHLTIER
                     LGGGLCPEDCIFVDDLPVNCDAARDLGMVAVLFETTAQARADIEAALAPG"
     gene            complement(9436..10140)
                     /locus_tag="VHF88_07515"
     CDS             complement(9436..10140)
                     /locus_tag="VHF88_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161653.1"
                     /translation="MSQPALSLNFFDPEHGLHGNARSGMTLLFEGSSSRALADGAQIE
                     AAGGGWRAELNGAFELDLESVAPAADLDGVTVHLCVATGKVGGNDVRCMATVSETHAA
                     PSWEELDALRSISALFDEGHAFLAIGRRPRGALGHDAERTVAWLLDDGQALPVEDARI
                     STVYDGDGRQRSAGLELWLADEDHALRGSGTVVAGSSLQLEAADVHAAIFRWRLGDRE
                     GAGSYELMTRSEPEAA"
     gene            complement(10137..10619)
                     /locus_tag="VHF88_07520"
     CDS             complement(10137..10619)
                     /locus_tag="VHF88_07520"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX2161654.1"
                     /translation="MPLLLIAVFIVVPIAELYVILQVGDAIGALPTIALLVADSLLGS
                     MLLRSQGRSVWRQFNEAMRAGRMPHRELFDGIAVIFGGAFLITPGFLTDVVGLLLLIP
                     PTRAVLRRFAVRRLGKRFGLGAVAGVAGRARRPRRPEDTVEGTAREHRDEPPQGRIGP
                     "
     gene            10657..10965
                     /locus_tag="VHF88_07525"
     CDS             10657..10965
                     /locus_tag="VHF88_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX2161655.1"
                     /translation="MSDIDVTVDDVREALENVIDPELGLDFVELGLVYDIAVEGTEVF
                     ITFTLTTPGCPIGPQVTEQMQEFVGDLPGITKVHPTLVFTPAWTPDLMSEDAKFALGY
                     "
     gene            complement(10962..11720)
                     /locus_tag="VHF88_07530"
     CDS             complement(10962..11720)
                     /locus_tag="VHF88_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161656.1"
                     /translation="MLLISDGEVVDPVWDSAVSRALMLRVAAKEVPETVRVSRPGASV
                     VFGKRDVVSEGYGPAIAAARDSGFEAVERLGGGRAAVFHEGTLHFGHAIHSAEPGPVI
                     QRFEETAELTARALKRLGADARVGEVPGEYCRGEHSVNARGVTKLVGLGQRVVRGGAY
                     VGGVLVVSGAERIREVLVPVYEALGLEWAPETTGSLEDEVPGVGWDDARRAIEAEYGE
                     RYELEPAELDGETLALAERLMPEHRSSPTGARRP"
     gene            complement(11828..13405)
                     /locus_tag="VHF88_07535"
     CDS             complement(11828..13405)
                     /locus_tag="VHF88_07535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX2161657.1"
                     /translation="MAQSHRDRLSAIDASFLHQERRSSHMHVGAIVLMEGPVPTREEF
                     TRHIESRLHLVPRYRQKLAFPRFEMGRPFWVDDPRFNIDYHVRHTALPSPGSLEQLRG
                     IAGRIFSQRLDRSKPLWEIWLVQGLADGGFALVSKTHHALVDGVSGVDIATVLFDLSP
                     TPPPREEPEPWVPEQEPSQAELVAEGIKGLMRTPSMVAGEAAKVVRDPATTVERAREA
                     LEGVGEIAWAGLNPAPDVPLNVEIGPHRRIVWLRCDLADFKAVKDGLGGTVNDAFLTV
                     VAGGLGKWLRSRGVRTEGLELRGLVPVSIRATDEHHALGNRIAAMRGPLPVYAKDPVD
                     RMRIVREAMKDVKESKQALGAEVIAGLEDFAPPTLLAQASRINFSTRLFNLLVTNVPG
                     PQFPLYMMGREMQEIVPVAFLPENHALAIAAMSYNGKLDFGLLGDYDAMPDLERMAEF
                     FEESLEELLGAARGKAGKKKPAGKAAAKKRTTKKPAAGKAKVKAAASNGASSNGKGAK
                     RSAIRGKAQRPRLIDSD"
     gene            13435..14334
                     /locus_tag="VHF88_07540"
     CDS             13435..14334
                     /locus_tag="VHF88_07540"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX2161658.1"
                     /translation="MGWQFDRRRAVATAAALAAAAMVAAACGDGNDGSAAAAERVPRP
                     TVDRFDSAAAYELVREQVELGPRPAGSKQSRVLAERLRRMLPNGRFQPVPGGLRNVVG
                     RVPGREPRRVVVVGAHYDTKEIPGFLGAVDGASGTAVVTELARTIRPRELRPTVVFLM
                     FDGEEVPAGKPDSQFTRYGIRGSKAAAPRYRDAEAMILLDFVGERGLRIPRESYSNEG
                     LWQRLRAAARAVGVAKVFPAEDFGAGIQDDHLPFIEQGVPSIDLIDFDFPCWHRRCDD
                     LSRVSERSLDASGETVYELLRRL"
     gene            14339..15295
                     /locus_tag="VHF88_07545"
     CDS             14339..15295
                     /locus_tag="VHF88_07545"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX2161659.1"
                     /translation="MAHVPEKLLLAAPRGYCAGVDRAVQTVERALELYGPPVYVRKEI
                     VHNKHVVEQLRERGAIFVDQESEVPEGETVVFSAHGVAPSVHANAEARELKTIDATCP
                     LVTKVHVEAKKFAADGYTIVLVGHAGHEEVEGTMGEAPDHIVLIETEADVDALEVEDP
                     DRVAFISQTTLSVDETRAIIARLREKFPNITGPRTDDICYATTNRQMAVRQMAGECDL
                     VLVIGSSNSSNSNRLVEVARVHGAESHLIENESHVREEWLDGKRVVGISSGASAPEEL
                     VQRLVDFFRARGTEDVAEFEVVQEDVRFMLPKEIRKALAAQV"
     gene            15345..18197
                     /locus_tag="VHF88_07550"
     CDS             15345..18197
                     /locus_tag="VHF88_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161660.1"
                     /translation="MRGWLIGVCAIALALAAAPGAHAQFGVDPDKWIAQVYGTDVETE
                     TQATQEYGQVGIYDQAGGHPWKGITDFTLRLGPDGNPAGGNPSNVRVDVPVGLVPNPT
                     IFPRCPRVLFDSGSGACPSDSQIGIEELTIKEGPLVAEVQVPLYNVEINPGEVSLFGF
                     HPADAAPVVPALATALAGMHPVYIVGGVRDQPSQFGPHDTGLFFTIDDAPATPPVLRS
                     KLTFWGVPGDPVHDVARDNPCVTIISGPLPAGRACTRPEAGVGIPSTVPDIPFLSNPT
                     KCGGVTLFTRLSLWSHSGAFASVDDPTPTINGRDGPQRCDRVPFDSAIEVTPNTAQPD
                     APVGPVVRHITPQPGLRAKDVITTSHVKDVSVTLPPGMTLNPSAANGLMACTDAQLAA
                     NPGVVGGDECPEASQVGTAAAKSPLLPDAVTGSAFVGQPLAGDKYRLFVTLEGRGVSV
                     RLKGSVRPDPQTGQVTTTFANNPEQPVDTFDIDIEDGSRAPLATPLDCGTKTASAVTT
                     PWSGTAPDSATSSFTIGAGGCSPGFAPAFGARTGSSTAGGFTPVTASIARADGDQFLS
                     GVRVDMPPGLGAIVKKMPKCSSTLAARGACPASTLIGTASTRSGAGAEPFPLSGPVYF
                     TEGYKGAPFGMVAVIRAIAGPYDLGTVVVRQSVFVDPEDAHITVVSDPLPQILEGVPI
                     RLRAVDVTIDRSKFVYNPTSCGRKSVGTTLHSIQGAVSRSSSSIATDRCQALRFKPRM
                     SMRLTGRRQVKLGKHPGLRVRVRQARGQANIGRARVVLPLSLALDPANARAICGFEAG
                     LKARCPAKSKIGRARAVSPALNRPLRGPVYFVQGIRIDPETGNRIRTLPSLLVKLNGE
                     IRVNLRGTTSVARRRLVSTFPTVPDAPVSRFDMRLKGGKGGILTVATRARRGICARRR
                     QISRAVFIGHNGKRKVQRVKMAKPCKKRKAPRKRRR"
     gene            18355..21477
                     /locus_tag="VHF88_07555"
     CDS             18355..21477
                     /locus_tag="VHF88_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161661.1"
                     /translation="MRFLGFCCALIGVLAASAPAAQAQQFGIENFTGMVFADETRNRT
                     DAYSNTGGRTTDAYPDPSTGGTGIYDLAGGHPFIGVTDFSFNRVSPTGPPAGGNVEEL
                     RVDIPRGLMPNPRAFARCTDAQLEALACPIESQIGSEELRLHISALAALGDITVHVPL
                     YNMTPLSHPEDPGPRRDTVARFAFHPAEAAEAISPLPPLPPLPPPLDTLLPAGLQGLA
                     AGLANLHSVHIVGGVRDEPTAFGPFDYGLFFTIDHLPAYNPANPASPGVVRTNLTFWG
                     APGDPAHNPAGPGQRGRGVSCVALGNLPNLGQFCTPIPSLAGAPDPSIPFLSNPTECT
                     NAPLVGRLTVFSHPQAGGQVLSDTRTDQTPAIVDRDDGQVKNGAQECQQLSLAPAIDL
                     RPGTDQPDTPVGPEVAISMANEGLANRNRFAESHAKDISVTLPPGLTINPSVANGLQA
                     CTDDQFAANVGVPAGEACPAASRIGDVRVISPLLPPAAGDDPNSTPVLTGSAYVGQPL
                     ANDQYRLFLTIEGREVSIRLKGSIQPDPNTGQITAIFRDNPQLPFDRLAVDFRDGSRA
                     PLATPLDCGPKAASTTVTPWSGTAPVTATSPAFNIGGASCPVPFRTNFDVATASPGSG
                     QFSPLSVHIGRGDRNQFLSKVRVDAPPGLAAKIKGVTQCEDAAANTGACPPASRIGTV
                     TTTAGAGSEPYRLSGPVYLTEDYKGAPFGMVAVIRAIAGPYDLGTVVVRQQLHVDPED
                     ASVTVVSDPLPQILEGVPIRLRSVDVTLDRRGFTYNPTSCGTKEIGGRMHSINGTVGE
                     HPNAFIRFAGCDRLGFTPRMRMRLTGARQMRQGGHPGLRARVTQRASQSNIASAVVRL
                     PKSLALDPANAQAVCGFEAAQRAKCPRKTRIGKATAVSPVLNRPLRGPVYFVQGIRID
                     PETGNRIRTLPSLLVKLNGEIRINLRATTDVRRGALVSTFPNVPDAPVSRFSLSLKGG
                     KGGVLAATGRPSICGRRQVAIARTVGHNGKRAKQRRVRMTTKSPCRKLKRIQAKAKKR
                     KAAAQRRARR"
     gene            complement(21474..21845)
                     /locus_tag="VHF88_07560"
     CDS             complement(21474..21845)
                     /locus_tag="VHF88_07560"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2161662.1"
                     /translation="MHFVVDGMNVIGTRPDGWWRDRDAAMARLVDQLERFSAASGDDV
                     TVVFERAPRPPLRSTVIAIEHAPRPKPDAADYKILELVKAHPSPATLHVVTSDNWLAD
                     AARDAGAVVEPSEGFRTRLED"
     gene            complement(21856..22647)
                     /locus_tag="VHF88_07565"
     CDS             complement(21856..22647)
                     /locus_tag="VHF88_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161663.1"
                     /translation="MRAPEGSDIRAPEFPPKMEWLNVAFLRMMKLVNREATLIEFFDV
                     ARINSHRTLPYLREWHARYGDRGLRVIGVHCPGYSFGTDRDVVERAVQRMGIEYAVLL
                     DPGFEVWREYANKGWPGRYLFDVEGRLRSIHYGEGDYLDCERAIQEVLLEIDGSLQLP
                     EPMEPVRPEDAEGVLLEAQTADIALPADQDRLELDGEWDEGEDYLEARAAGATARPGS
                     FSAGAAYAVVAGAGVDEPGLRQTDGPVEAPQPGFRLYGYQFTPKT"
     gene            complement(22671..23378)
                     /locus_tag="VHF88_07570"
     CDS             complement(22671..23378)
                     /locus_tag="VHF88_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX2161664.1"
                     /translation="MSGVNDLVLVRGMADTRVALREWNERPGRILWPWVALSFAVACA
                     LLLAVYLVAKAIPPDPTLLRLPGVNDSATAADYGRILYRNGLVLALHAMACVAGFIAG
                     SSLPLSAANRSGVDRWVHEKAGPLAIGFVICATLFSLTTQAFGIGILASDVAGQLGIG
                     PGTLILGLLPHAIPELTALFLPLAAWIIASRRRRWDELLAATFVTVALAVPVLLVSAA
                     VELWVSPELLRALAGLS"
     gene            complement(23417..24478)
                     /locus_tag="VHF88_07575"
     CDS             complement(23417..24478)
                     /locus_tag="VHF88_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161665.1"
                     /translation="MSVRVAAVEKRFGATRAVAGVDLSLEEGELVAVLGPSGCGKTTL
                     LRLIAGFEAPDAGTITVGGEVVAGAGRSVPPERRRIGMVFQDYALFPHLRVDANVSFG
                     LARRSTRERRERTRETLALVGLEQKADRYPHELSGGERQRVALARALAPEPEVVLLDE
                     PFSSLDATLRAGLRRDVETILREAATTALLVTHDQEEALSLADRVAVMRDGRIQQDGT
                     PEEVYGRPTSQWVTSFLGDAEILPGKAAGGAVDCELGRFPIPAELEGEVDVVVRPESV
                     ALGQGPAPRGDRGHEGVVVAREYYGHDQLVVVQLASGRKVRSRSMGFPIWHPGDRVRV
                     WVDGPVNALRREPTADAAG"
     gene            complement(24475..>26042)
                     /locus_tag="VHF88_07580"
     CDS             complement(24475..>26042)
                     /locus_tag="VHF88_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX2161666.1"
                     /translation="PARRVAVPADGWTVATVLVAAAVAGPLIVLPASFLAAPDALSDF
                     AGLLPDALIATALLVAGVGAGTLALGTGLAALVVFCDFPGRRWIEWALVLPLAMPGYV
                     FTLFALGSADAVGLSLTTVRSTAGAVAVFTLVLYPYVYLLARAAFLAQAGDMIEAARG
                     LGLTRLQAVLRIAVPLARPAIVGGVALALMEALADFGTVNLLGVQTFTNAIYKVWFGA
                     FDRDAALQLGAMLLTITLALLMLERISRGRARYAQGTRRGAMPVRLHGPAAVAAVAGP
                     LLLLALVVLAPVAQLGAWSLASIRDGLLAPEFALAARNSLMLAGMSIAIVVAVALVLA
                     YGRRASSSRTSAAALRVSTIGYGLPGSVVAVAVIVPLGWLDHRLDDAAGVGLLLTGTV
                     IGLFAAYTVRFLALAFQSVDTGLERVPRSLDEAARGLGSDRLDVLARVHVPLIAPALL
                     TAGLLVFTEVMKELPATLLLRPLGGDTLAIAVWQATTESLYETAALPALLIVLVGLIP
                     VGVLIRLSRRAAS"
BASE COUNT         4133 a         9338 c         8788 g         3783 t
ORIGIN      
        1 gggagcgcgg agaggagcgt ccagggctcg accccggccg tcctcgcggc ggcgcgcagc
       61 accaccagcg cgtcgagcga caggccgccg agcggcgacg ccagcgccgt gtagagagcc
      121 aggtcgtcga gcgggttcgc cagcgcggcg aggtacgcgc gcaggtccgc cgtctgctgc
      181 tggccccagt agccgcgcgc gccgaccgcg tacgtcggca gcccacgctc ggacagcgcg
      241 cgctcgagga ccgcggcatc ccgacttacg tgatgggcgg acgcggctac tggtcccagc
      301 agcaggtcgg cgacctgcgc gcctacctcg ccgcgctcgc gaacccgctc gacgacctgg
      361 ctctctacac ggcgcttgtc tcaccgctcg gcggcgtgtc gctcgacgcc ctggtcgtcc
      421 tgcgcgccgc ggcccaacgg gcccgcgtcg agccgtggac gctgcttgcc gagctcgccg
      481 gcgtctccgg cggccgggtg gcggtcgacg gcgggtcgcg cggcgggcgc gcccaggagc
      541 tggccgccca gctcggcgac cgcgacgccg agcgcgcccg cgccttcgtc cggctgctcg
      601 ccgaggagcg cgccgccgcg ccgcgctcct cgctcgaggc gctgatcgac cgcggcgtca
      661 cgcgctccgg ctacgacctc cacctgctcg cccttccggc cggccggcgc cgcatggcca
      721 acgtccgcaa gctgatgcgc ctcgcgcgcg agttcgaggc cgaggagggg cgcgacctgc
      781 gccgcttcat cgactacgtc gccgagcgcg acgagctcgg cgagcgccaa ggggaggccc
      841 cgctcgagcc cgaggacgtc caggccgtcc gcctgatgac cgtccaccgc gccaaggggc
      901 tcgagttccc cgtcgtctgc gtcgccgatc tcggcaagcc gggccgcgag gaccgctccg
      961 cgctccaaat ctccgacgac ggcgccgtcg gcatcagcct cgctcagctc ggcggcggca
     1021 gcgtgacgag ctcacggctg aaggagatcc gcgagcgcca gaagctcgcc gacgaggagg
     1081 aggagaagcg cgtcttctac gtcgccgcca cgcgcgccga ggagcacctg atcctcagcg
     1141 gcggcaccga cctggagaag ctttccgagg agaagccgct cgaagagccg atgcgctgga
     1201 tctggcgcgc gctggcgccg gggttggggg agatcgacac gagcgctgtg gtcgagcacg
     1261 agctcgacgg acggccagtc gcagtccgtt gcaccgttct ccgccccgac acggtcgacg
     1321 cggtcctccc agccgcagac cggaccccga gtgcgcccga gcaaccaccc gccggcctcg
     1381 acgccctgga cgcgcccgcc ctcgccgcgg ttgcgccccc gaccgccctg ccgatcagcc
     1441 ggatcagcta ctccgccttg cagcgccacc gcgagtgtgg ctaccgcttc tacctcgagc
     1501 gctccctgcg gttgccccgg ggagaggccc tgcccgagcc tgccgagcca gaggccgagg
     1561 ccgtcgacgc cctgcctccg accgtccgcg gctcgatcgc ccacagcctg ctggaggaga
     1621 tcgacttcgc ccagcccgtg acgcccaccg tcgaggaggt cgccgcgctg atcgaagccc
     1681 accgcgagcc ggtccgcgac gacgaggtcg ccgacatcct cggcctgttc gagagcttcg
     1741 ccgcctcccg gctccgccag cgcctcgccg ccgcccactc cgtccgcacc gagcttccct
     1801 tcgccttcga gctggcgccc gacacagcgg gcggccgcac cctgctcgtc aacggcatcg
     1861 tcgacgtcca cgccgtcgag tccgaccgca cgctgatcgt cgactacaag agcaaccgcc
     1921 tcgaaggcgc gacccctacc gagatcgtcg agtccgacta cgagatccaa cgcctcgtct
     1981 acgccctggc cgccctccgc accggcgctc cccgcgtcga ggtcgcctac gtcttcctcg
     2041 agcgcgccgc cgaccccgtc tcagccgtct acgaggcctc ggccctcgaa gacctcgacc
     2101 gcgagctccg cgacatcgcc gccccggtcg ctgccggtag tttcatccca acgccccaac
     2161 cttggcgcgg cctctgcgcc gactgccccg gccagcccgg cctctgctcc tggccgcccg
     2221 agcggacgtt ggctcatagc cactcaggcg gatgaccacg tggccacgcg agcaagctat
     2281 tccaagtctt acgggtttcg cgtcaagtag ggcggcgctt gtcgcgtaag taaaaggccc
     2341 tagaaagcgc gctgatggag ctgagagaca ccgcccactg cctcgacttt cttcgggcag
     2401 acgaaccgcc cgctcgtgtc gcagcacccg ccacgcgagg acgttaggag cgcggaaacg
     2461 ctcaaccgga gtgggaaccg aaatcggttc acgccaactc ctccctaaaa agtgccctct
     2521 atgcggggac ttggcaggat gctggccgga tgcacgagta cggtcgtgtt cgtgtttgag
     2581 ctgattgcat cgtggtcaga ggacatcggg aacctcaccg ggccgctcgc catcgtcgcc
     2641 ttggccttgg tcttctcgct gtacaggaac gagattcgag ggtttctcgc gaacgcgagc
     2701 aagcttcggg ttcaagagcc cggtggcgag ctggagatga cctcacagcg ggatagcgac
     2761 tcagaggacg cggagacctc tgagcccgcc gagagcgctt cctctgcgga cccgaacgag
     2821 gagctaaacg cgccgaagac ggaggttgag ggcgccgctg ccaccgacgc cgactacgag
     2881 agcgagacgg acaccgacaa gctccgcgtc cgtatgggca acgcgctgga ggctaaggac
     2941 gaggagaagg cggaggccat cttcaaacgg ctccagcagg ttgaggagaa ccctgtccag
     3001 gggaaggttg accaggggct ccgcctctgg ttgcggttcg tcttcaagtt cgagccggat
     3061 gccttggtca agctggagga gctggcggcc gacgagcggg tagcagctca catgtacctg
     3121 ttccgggggt actgtctctc tcgtgcgggc aacacgagtg gcgccgcttc tgagctcaag
     3181 cgagcgcgcg atgccgcaac gagctcggac gtgaagacca gggccaccat cttctatgcc
     3241 aaggagctcg acaaggccgg cgaccaagcg caatcgttac gcgagataga gaccgcgcta
     3301 cgcgagtgcg aggacccgag cttgcgggcc agcctttggc gcgccctcgc agagctctac
     3361 gagcagcacg accaacacga gttgcgggcg cttgccttgc aaaaggcact agaggccgct
     3421 cccgacaatg cgcggctacg gcttgatgtc gcttacgcct actcccacgc ggacgagagt
     3481 gacttcgggc ctctggtcat ccaccactac gagacagccc ttagcttcga gccgacctac
     3541 gagactgctc gcaacaacct gggcgttgct tacaaccatg ccgacctgac catcctgagc
     3601 gccaaggcgc tgaaacgcgc cagcgacgat ggaagcggtc tcgcgacagg caatctcgcg
     3661 ttccgctacc tcaacgcagg attcgcagac caagccgagg agctggttaa agaggcgacc
     3721 aaaggcggga gccccctgca tgcgaaggtc gccgaggcca gtgccgacat cgcccagcga
     3781 cgcgagagcg agaacgagca gcgaactcgt ctatcggcac taggtcagag gcaagcagcc
     3841 ttcctcagcg attacgccga ggcccaagtg aaacccgcaa cctccctcct cgggacgtgg
     3901 cgcttccagg acagcgggga tgaggtcacc gtcactggcg agaacgattc gctcttgggg
     3961 ggagtggact gtaggaggga ataccaagtg gaagttcgag ggaaccagcc acgggcagac
     4021 cgcgcgtctc aaggtctccg agatgcagta ctggacctgg agcggaaagc gagaggagcg
     4081 cagcttctcg acccaggcgc aggccttcgc gtacctaaca ggtgatggtc gcttcttgga
     4141 gatgatggag gctgccggtg ccgacgtctc gttccgcacc cttgagcgcg tggagaagca
     4201 ggtcgcagag gccgcctgag catcgcgtgt ccgacgacgc caattcgaag gcgagtgcga
     4261 ggagcgtcgg atggtgggcg ccatcaatga cgcgcgggcg gcggaagact accgcttctc
     4321 gtcatctccg gctcgctctc acgagcggcg agtgaccacg ccgctcgacc gagcgatggc
     4381 ggccgggtgg ccgagcaacc aagcatggga ggtcctgaat aaggcggaga acccggcgct
     4441 cgtgttcgag acgtgaatgg cgagcgcgag ccaccgggcc atcctcatgt cgccggccgt
     4501 ggactccgtc ggcgtcgccc gcgaccccgt cgctggctac tactgggttt ggtctcgccg
     4561 gccgctgtcc cgccgaccgt ccaccggcgt gcgagctgac ccgcgacacc ggagacgccg
     4621 ggctcgcgct cgaagcacgc ggcgacggct cgctcgtgtc cgtgtggcgg gcaggcgctt
     4681 cgtggttcga gcccggccgg ccgctggcgc gccgactagc cctcgtcgag ggcgcccggg
     4741 aggtcgtgcg cttcgacggc ccggagggct gggccgacca acgcaccacc actgaacggt
     4801 ccgtgtcagg tagccctgac acgtccgctc ctgcgactac gtttcgatgc atggaccgcg
     4861 agacggcgaa cgtgcttatc gtggactggc gctgggaggg accggcgaat gaccaaccct
     4921 ccaccgacgc gtcgcgtcgc ctcactcagc tcatgcttga gctagttgga cagcctgagc
     4981 tggtctctgg ggtgacgctg gaggacggca gtcgagccgc actcgttctg cacgagggca
     5041 gcttgttcat cactacggcg accggcgagg gaagcgatgt tcaagtctca gtacgtcggg
     5101 agcctctgga ctccaaccgc gttctcgtga cgatgaatga gcgcaaggaa ggccaacccc
     5161 cggtgacatg ggtgcgtacg tggaccttcc agcgcgtggg ggaggagcct ttcttgagct
     5221 tcctcacgca cagtcatcgc cgtgatgaga aagagccacg tgccaccgcg ttcgcacgaa
     5281 ggctgtgccg cacagttggc tgggacatac cggtcgagga gccccgttga acggtccact
     5341 ttgcctcgat acggtggctt ccgtgaacga gctcagccga taccgcgact cactgccgcc
     5401 tgtttgggcg gttcctgagc taggtctatg ccagctcacc aagccgtcgc cggctatcga
     5461 cagcgtgttg agctcctact acgaggaccc tgagcacgcc gatgcgggcg ggtacataga
     5521 cgcactcatc agtcatgtcc tgcgtagtcc tcgtctgagc tatgacgagg tccgcgacct
     5581 ggctgacccg gttcgcgctg agctgcgggt cgcttgcgcg gtgtatgcgg gtgtcggtct
     5641 cccgcccgag agattgactg accgccgcct cttggtagcg atggccgagc ggttccgtcg
     5701 ccaggcgtcg cagccttccg atggtggctt cctcggcttc tttcggtcgc tccaaggttt
     5761 gggcgagcac gcggccgcgc agcttcatca ccggttgcgc ggcgtccctt ttgaggtcgc
     5821 tgcgcaagcg gtcgcggatg ccttcgagga cttcaggaaa gaccaagagc gcaagctcga
     5881 tgaggtacgg cgtcagccgt tgttcttcat cgtcgctgac ctccctgtga gcgatgcgcg
     5941 gtggctgctg ttcgaggcgc ccgagaacat cgcgacgctt gtccgggaga ccgtgatgac
     6001 cgacccaacc ttcctcgaca acgtcgagcg ggcggtgctc gatgctccgc atctggacga
     6061 gccgcaacga gacgacctcc tcaagggctt gaaagcggtt cgcaatggcg acccctacgc
     6121 atgtcgccat ttgctggcag gcgtcgaggg tggcttgtgg ctggctgccg aggatgaggg
     6181 ggtcatcgac agcaaccggc tccttctgcg ctccaagaaa aagaacgagc cgttcgctcg
     6241 caacttcggt cgtttcctcg acaccgacga tggcggactt gacgtgggcg atgactactc
     6301 gtggttccta cgtcgcgctg cgtacgacga gcaaggtcaa gcgattcggc acggtcgtgc
     6361 gaggatcggt cacgcggact actccgtctg gagctttgtg gctctactcg gctggctcga
     6421 ccgcttctcc ggcaccacct tcatgtacga ggtcacagac cgcttcatga tggagcccga
     6481 cgagaccgag acggagatgg tcggccaaga gcctaggtga ggtaggacgc gcttggggac
     6541 tgccgcgggg cttgctgacg attgccgagc aatgcggcct ccggcacgga ggcgtctcgc
     6601 gggcgcactg cgccgcgtga tgtcagcgcc ccgcggctgg gcgcggcggc ggactgggtc
     6661 gaggcgccgg acggtcctta ccgtgggtct cgggccgcat cgggcttggt cggacgggct
     6721 cggggcgagg cggtggcttg ggctgtccgc ccgatccgat ttgggttgcc atggcgtcag
     6781 tctaccttcg tggcaaggtc gatcgtccac gtcacgatct ctacgacgag caggatcccg
     6841 gccgccctga agctccagac gaggcgtcga aactgcttgc cgttctcctc gagcacggag
     6901 gcacgatgga gggcgagatc ccgctggatg tccactacgg gcaggggctc ctctgcctcg
     6961 atgtacgtcg cgacgatgtc ggccggatcc gcctcgaatt tccagttccg gggccagagg
     7021 atcgccaggg tcgccagacc gagcagcgcg aagacggcga tggaaatcca gctccagacg
     7081 ccggggttgc cggttcgtag agcttgtcct ccaagaaagg aggtcgcgat ggccgcgccc
     7141 gacaggagca agcccgcgcg accgcggaag ctgtcgatcg cggcttgctg actcgagaca
     7201 gcgagtaccg cctcgtcata tgccagctcg taggcgttct cggcgccgtc atgggaagcg
     7261 gttgggggca ccgttcaata atgcaaatat gctcggtcgg aaggccctct ttctcgaagg
     7321 tcgcattccg tccaccacgg cccctacctt ctgggcccat gcccctctgc ttcatcgagc
     7381 cacaagaggc catccgtgtg atcgcagacg ctgtcgggcg agaggcccgt cggcagctgc
     7441 ttggcgaagt cgtgcgcgcg tgggtcgacg agatcccgga cgacgagctg gcggttcact
     7501 acgcgaaggc cgtcgaggtg ctcgacgacg acgacctgtc gatgctggcg gcctggcatg
     7561 cgtcgctcga ggtcgggcag ccgatcgcga acaaggactt cctgctcaag gcgttcggat
     7621 cgcttggcga gcatcggcgc gaggctctgc ggatcgccgc aggtttccgc ggcgacgacg
     7681 cgttcgaggc cggggtcagc gaggagcagg cgatcgacac ggtccttccc ttcctgtccc
     7741 atgagcggat gacggagctc gccgctgagg cgatcgagct ctactgttgg gaccgcatgg
     7801 gcagcgacaa ctgacggcgc gcggacgcgc ctgacggcgt cctcggtcgg ccatgggctg
     7861 acgccctatg gcctccctgc gtccttgtca gccacgcccg cgcactcgtc agtcgcgctg
     7921 cccgggctcg tcgccttgac atattccggt atcggtatat attgaggtca tggcacgggc
     7981 ggcgactacg gcggacgcgt tcaacgcggt ggcggagcct cggcggcggg agatcctgga
     8041 tctcctggcc ggtggggaga gcgccgtgaa cgacatcgtc gagcggctcg ggttgaccca
     8101 gcctcaagtc tccaagcacc tgcgggtgct gcgggaagtg gggctggtcg acgtacgcga
     8161 cgagggacgg cagcggctgt atcggctgaa cgggccggcg ctcaagccca tccacgactg
     8221 ggtgagcagc tacgagcgca cgtggtccga gcggttcgag cggctcgacg ccgtcctcga
     8281 ggacctcaag cgaaaggacg aaggagatga cctgcatgac tagcagcggc aaggcgaacg
     8341 tgacgctgcc gaccgacgag cagatcctga tcacgcgcga gttcgacgcg ccgaaggagc
     8401 tcgtctacca ggcgtggacc acgccggagc tggtgcggcg ctggtggagc ggcaaccgcg
     8461 gcgagatgac cgtctgcgag atcgacctgc gcgtcggcgg caagtggcgc tacgcgatgg
     8521 tggcgaacgg gggacaggag gtcgccttcc acggcgagtt ccgcgagatc gtcccgaacg
     8581 agcgggtcgt ctcgacggag gcctacgagg gcatccccga cgccgacgag cacgccacgc
     8641 tcaacaccct gacgttcacc gacctcggcg gcgggcgcac gctgatggag gtcctcgtcg
     8701 cgcacccgac caagctcggg cgcgacatgc acatcgactc cgggatggag atcggcctcc
     8761 aggaggcgct cgaccacctg gaggaggtgg ccgtctcgct gcgctcctag ccgggggcga
     8821 gagcggcttc gatgtccgcg cgcgcctggg cggtcgtctc gaacaggacg gcgaccatgc
     8881 ccaggtcgcg cgcggcatcg cagttgaccg gcaggtcgtc gacgaagatg cagtcctcgg
     8941 ggcacagacc gccgccgagc cgctcgatcg tcaggtggta gatctcgggc tccggcttgc
     9001 gcatcccgac gaacgccgag tcgaccacca cctcgaagat ctcgtcgatc tccggcagca
     9061 tcgcgcgcca gcgcgcctcc cactcgcgga cgttgttggt cagcagcgcg gtgcgcaggc
     9121 cccgatcgcg cagatcggca agcagctcga tcatctcgtg gtttcgctcg aggcccgcca
     9181 tgaacgcctc tcccatgccg tcgagccgct ggccgccgag ctcctgctcc agccgcgcca
     9241 tgaactccgg ctcggtgagc tcgccacgct cgagcaggaa cagcgggtgc tcgccgtggc
     9301 gctcgtgcga ggcgaccagc gccttgccga ggtcctcgag cgcgacgccg ctctccttcg
     9361 accaggccgc gaaggcgttg ccgagcggcg tcgtcaagac gccaccgaag tcgctgatga
     9421 ccgccttcgg agcgctcacg ccgcctccgg ctccgagcgc gtcatcagct cgtagctccc
     9481 ggcgccctcg cggtcgccca gccgccagcg aaatatcgcc gcgtggacat cggccgcctc
     9541 gagctgcagc gacgaccccg cgacgacggt ccccgagccg cgcagcgcat ggtcctcgtc
     9601 ggccagccac agctcgaggc cggcgctgcg ctgacggccg tcgccgtcgt agacggttga
     9661 gatccgcgcg tcctcgaccg gcagcgcctg cccgtcgtcg agcagccagg ccaccgtccg
     9721 ctcggcatcg tggccaaggg ccccgcgcgg gcgacggccg atcgccagga aggcatggcc
     9781 ctcgtcgaac agggcggaga tcgagcgcag cgcgtcgagc tcctcccagc tcggcgccgc
     9841 gtgcgtctcg gacaccgtcg ccatgcagcg cacgtcgttg ccgccgacct tgccggtcgc
     9901 gacacagaga tggacggtca cgccgtcgag gtccgcggcc ggcgccaccg actccaggtc
     9961 gagctcgaag gcgccgttga gctcggcccg ccagccgccc ccggccgcct cgatctgcgc
    10021 accgtcggcc agggcacgcg agctcgaccc ctcgaacagc agcgtcatcc ccgagcgcgc
    10081 gttgccgtgc aacccgtgct cggggtcgaa gaagttcagc gacagcgccg gttggctcac
    10141 gggccgattc tcccctgtgg cggctcgtcg cggtgctcac gtgccgtccc ctcgaccgtg
    10201 tcctccggtc ggcggggccg ccgcgcgcgg cctgcgaccc ctgcgaccgc gcccagcccg
    10261 aaccgctttc ccagtcgccg aacggcgaag cgccgcagca ccgctcgggt cggcgggatc
    10321 agcagcagca gaccgacgac gtcggtcaga aagccaggcg tgatcaggaa cgcgccccca
    10381 aagatcaccg cgatcccgtc gaacaactcc cgatgcggca tccgcccggc gcgcatcgcc
    10441 tcgttgaact gccgccacac cgaccgcccc tggctgcgca gcagcatcga ccccagcagt
    10501 gaatcggcga ccagcagcgc gatcgtcggc agagccccga tcgcgtcccc gacctggagg
    10561 atcacgtaca gctccgcgat cggcacgacg atgaacacgg ctatgagcag gagcggcatg
    10621 cggctccgca ccctacccac gactaacatt ggccccatgt ccgatatcga cgtgaccgtg
    10681 gacgacgtgc gcgaggcgct cgagaacgtc atcgaccccg agctcggcct cgacttcgtc
    10741 gagctcggcc tcgtctacga catcgccgtc gagggcaccg aggtcttcat caccttcacg
    10801 ctcacgaccc ccggctgccc gatcggcccc caggtcaccg agcagatgca ggagttcgtc
    10861 ggcgacctcc ccggcatcac caaggtccac ccaaccctcg tcttcacccc cgcctggacc
    10921 cccgacctga tgtccgagga cgccaagttc gccctcggct actaagggcg gcgcgcccca
    10981 gtaggcgagg accggtgctc cggcatcagc cgctccgcta gcgcgagcgt ctccccgtcc
    11041 agctcggccg gctcgagttc gtaccgctcc ccgtactccg cctcgatcgc ccgtcgcgcg
    11101 tcgtcccagc caacccccgg gacctcgtct tccagactcc ccgtcgtctc aggcgcccac
    11161 tcgagtccca gcgcctcgta caccggcacc agcacctcgc ggatccgctc ggcgccactc
    11221 acaaccagca cgccgccgac gtacgcaccg cccctgacga ctcgttgacc gagtcccacc
    11281 agcttcgtca ctccgcgcgc gttgacgctg tgctcgccgc ggcagtactc ccccggcact
    11341 tcaccaaccc gcgcatcggc tcccaagcgc ttcaacgccc gcgccgtcag ctccgccgtc
    11401 tcttcaaacc gttggatgac gggtcccggc tcggctgaat gaatcgcatg cccgaagtgc
    11461 agcgtgccct catgaaacac cgccgcccga ccgccgccca accgttccac cgcctcaaac
    11521 ccgctatccc gcgccgccgc aatcgcaggc ccatatccct cgctcaccac gtcgcgcttt
    11581 ccaaagacga cggacgcccc cggacgcgag accctcaccg tctcaggcac ttcttttgca
    11641 gcgaccctga gcatcaacgc ccgcgacacc gccgagtccc agaccggatc gactacctcg
    11701 ccatcagaga tcaacagcac gacagacgcc acttccgcgg gtggcgacga ttttgcgttt
    11761 gcgcgcgcag cttgcgagca cgtaaaccaa aatcggagac aggacccgcg gcccccacgg
    11821 cccagcccta gtcgctgtcg atcaaccgcg gccgctgagc cttgccgcga atcgccgacc
    11881 gcttcgcccc cttgccattg ctggaagctc cattcgaggc agccgccttg accttcgcct
    11941 tcccagccgc gggcttcttc gtcgtccgct tcttcgcagc agccttccca gccggcttct
    12001 tcttccccgc cttcccccgc gccgccccca acagctcctc caacgactcc tcgaagaact
    12061 ccgccatccg ctccaggtcc ggcatcgcgt cgtagtcccc gagcagcccg aagtccagct
    12121 tcccgttgta gctcatcgcc gcgatcgcca gcgcatggtt ctccggcagg aacgccaccg
    12181 ggacgatctc ctgcatctcc cggcccatca tgtagagcgg gaactgcggc cccggcacgt
    12241 tggtcaccag caggttgaac agacgtgtcg agaagttgat ccgcgacgcc tgggcgagca
    12301 gcgtcggcgg cgcgaagtcc tcgagtccgg caatcacctc ggcgccgagc gcctgcttgg
    12361 actccttgac gtccttcatc gcctcgcgca cgatccgcat ccggtcgact gggtccttgg
    12421 cgtagaccgg cagcgggccg cgcatcgcgg cgatccggtt gccgagcgcg tggtgctcgt
    12481 ccgtcgcgcg gatcgagacg gggacgaggc cgcgcagctc gagtccctcg gtgcgcacgc
    12541 cgcgcgaacg cagccacttg ccgagaccgc cggcgacgac ggtcaggaag gcgtcgttga
    12601 cggtcccgcc gagcccgtcc ttgaccgcct tgaagtcggc caggtcgcag cgcagccaga
    12661 cgatccggcg gtgcggcccg atctcgacgt tgagcggcac gtcgggcgct gggttgaggc
    12721 cggcccaggc gatctcgccg acgccctcga gcgcctcgcg cgcccgctcg acggtggtgg
    12781 cggggtcgcg cacgactttc gcggcctcgc ccgcgaccat cgacggggtg cgcatcagcc
    12841 ctttgatgcc ctcggcgacg agctcggcct gcgagggctc ctgctccggc acccacggct
    12901 cgggctcctc gcgtggcggc ggcgtggggg agaggtcgaa gagcacggtc gcgatgtcga
    12961 cgccggacac gccgtcgacg agtgcgtggt gggttttcga cacgagcgcg aagccgccgt
    13021 cggcgaggcc ctggacgagc cagatctccc agagcggctt ggagcggtcg agccgctggg
    13081 agaagatccg accggcgatc ccccgcagct gctcgagcga gccgggcgac ggcagcgcgg
    13141 tgtggcggac gtggtagtcg atgttgaagc gcgggtcgtc gacccagaag gggcggccca
    13201 tctcgaagcg cgggaaggcg agcttctggc ggtagcgcgg cacgaggtgg aggcgcgact
    13261 cgatgtggcg cgtgaactcc tcgcgcgtcg gtacgggtcc ctccatcagc acgatcgcgc
    13321 cgacgtgcat gtgcgacgag cggcgctcct ggtgcaggaa cgaggcgtcg atcgcgctca
    13381 gtcggtctcg gtggctttga gccatcggtt gcagcctacg ccacaatccc cggaatggga
    13441 tggcagttcg atcggcggag ggcggtcgcg acggcggctg cgcttgctgc tgccgcaatg
    13501 gtcgccgcgg cctgcggtga cggcaacgac ggctcggcgg ccgccgccga acgggtgcca
    13561 cgaccgacgg tcgaccgctt cgactcggcg gccgcctacg agctcgtgcg cgagcaggtc
    13621 gagctcggcc cgcgtccggc gggctcgaag cagtcgcgtg tgctcgccga gcggttgcgg
    13681 cggatgcttc cgaacggccg cttccagccg gtccccggtg gcctgcgcaa cgtcgtcggg
    13741 cgggttccgg gccgcgagcc gcggcgcgta gtggtcgtcg gtgcgcatta cgacacgaag
    13801 gagatccccg gcttcctcgg tgcggtcgac ggcgcctcgg gcacggccgt cgtcaccgag
    13861 ctggcacgga cgattcgtcc tcgcgagctg cgcccgactg tcgtctttct gatgttcgac
    13921 ggcgaggagg tcccggccgg caagcccgac agccagttca cccgctacgg gatccgcggc
    13981 agcaaggcgg cggccccgcg ctaccgcgat gccgaggcga tgatcctgct cgatttcgtc
    14041 ggcgagcggg ggctgcgcat cccgcgcgag agctactcga acgaggggtt gtggcagcgg
    14101 ctgcgcgcgg cggcacgggc ggttggcgtc gcgaaggtgt tcccggccga ggacttcggc
    14161 gccggaatcc aggacgacca cctgccgttc atcgagcagg gggtgccgtc gatcgacctg
    14221 atcgacttcg acttcccgtg ctggcatcgg cgctgcgatg acctgtcccg ggtctccgag
    14281 cgcagcctcg acgcgagtgg tgagacggtc tacgagctcc tacgtagact ctgacggaat
    14341 ggcccacgta ccggagaagc tgctgctcgc ggcgccgcgc ggctactgcg ccggcgtcga
    14401 ccgcgccgtg cagaccgtcg agcgcgcgct cgagctgtat ggaccgccgg tctacgtgcg
    14461 caaggagatc gtgcacaaca agcacgtcgt cgagcaattg cgcgagcgcg gggcgatctt
    14521 cgtcgaccag gagagcgagg tcccggaggg ggagacggtc gtcttctcgg cgcacggcgt
    14581 ggcaccgagc gtccatgcga acgccgaggc gcgtgagctg aagacgatcg acgcgacgtg
    14641 cccgctcgtc acgaaggtgc atgtcgaggc gaagaagttc gccgccgacg gctacacgat
    14701 cgtcctcgtc ggtcacgccg gtcacgagga ggttgagggc acgatgggag aggcgcccga
    14761 ccacatcgtg ctgatcgaga ccgaggccga cgtcgacgcg ctcgaggtcg aggaccccga
    14821 ccgcgtcgcg ttcatctcgc agacgacgct gtcggtcgac gagacgcgcg cgatcatcgc
    14881 ccgcttgcgc gagaagttcc cgaacatcac cggtccgcgc accgacgaca tctgctatgc
    14941 gaccaccaac cgccagatgg ctgtccgcca gatggccggc gaatgcgatc tggtgctcgt
    15001 gatcggatcg tcgaactcct cgaactccaa ccgtctagtc gaggttgcgc gtgtgcacgg
    15061 cgcggaatcc cacctgatcg agaacgagtc ccacgtcagg gaggagtggc tcgacggcaa
    15121 gcgggtcgtc ggcatcagct ccggcgccag cgcgcccgag gagctcgttc agcgcctcgt
    15181 cgacttcttc cgcgcgcgcg gcaccgagga tgtggcggag ttcgaggtcg tccaggagga
    15241 cgtccgcttc atgctcccca aggagatccg caaggctctc gcagcccagg tgtgagtttc
    15301 gtcacataag gggagaaccg agccagatgc ggttcgggag agcgatgcga ggctggctca
    15361 ttggcgtttg cgccattgcg ctcgcgctcg cggcggcgcc gggcgcacac gcccagttcg
    15421 gcgtggatcc ggacaagtgg atcgcccagg tctacggcac cgacgtcgag acggagacgc
    15481 aggccacgca ggagtacggc caggtcggca tctacgacca ggcgggcgga catccgtgga
    15541 agggcatcac cgacttcacg ttgcgcctcg gccccgatgg caaccccgcg ggtggcaatc
    15601 cctcgaacgt ccgcgtcgac gttcccgtgg gcctggtccc caacccgacg atcttcccgc
    15661 gctgcccgcg tgtgctgttc gacagcggca gcggcgcctg cccgtccgac agccagatcg
    15721 gcatcgagga gctgacgatc aaggaggggc cgctcgtcgc cgaggtgcag gtaccgctct
    15781 acaacgtcga gatcaacccc ggcgaggtgt cgctcttcgg cttccatccg gccgacgcgg
    15841 cgccggtcgt gccggcgctc gccacggcgc tcgcggggat gcacccggtc tacatcgtcg
    15901 gtggcgtccg cgaccagccg agtcagttcg ggccacacga caccgggctc ttcttcacga
    15961 tcgacgacgc tccggccacc cctcccgtgc tgcgttccaa gctcaccttc tggggcgttc
    16021 ccggcgaccc ggtccacgac gtcgcacgcg acaacccgtg tgtgacgatc atctccgggc
    16081 ctctgccggc cgggcgagcc tgcacgcgcc ctgaggcggg cgtcgggatc ccgagcacgg
    16141 tcccggacat ccccttcctg agcaacccga ccaagtgtgg aggcgtgacg ctgttcaccc
    16201 ggctgagcct gtggtcgcat agcggggcgt tcgcctcggt cgacgacccg actccgacga
    16261 tcaacggcag ggacggtccg cagcgctgtg accgcgttcc gttcgactcg gcgatcgagg
    16321 tgactccgaa caccgcacag cccgacgcgc cggtcggtcc ggtcgttcgc cacatcacgc
    16381 cgcagcctgg cctgcgcgcc aaggacgtga tcacgacttc gcacgtcaag gacgtgtcgg
    16441 tgacgctccc acccgggatg acgctgaacc cctcggcggc caacggcctc atggcctgca
    16501 ccgacgcgca gctcgccgcg aaccccggcg tggtcggcgg cgacgagtgc ccggaggcgt
    16561 cgcaggtggg gaccgcggcg gcgaagtcgc cgctgctgcc cgatgctgta acggggtcgg
    16621 ccttcgtcgg acagccgctg gccggtgaca agtaccgcct gttcgtgacg ctcgagggcc
    16681 gcggcgtgtc ggtccgcctc aagggatccg tcaggcccga cccgcagacc gggcaggtca
    16741 cgacgacctt cgcgaacaac cccgagcagc cggtcgacac cttcgacatt gacatcgagg
    16801 atggctcgcg agcgccactg gcgacgccgc tcgactgcgg caccaagacg gccagcgcgg
    16861 tcacgacccc ctggtcgggc acggcaccgg atagcgcgac gagcagcttc acgatcggcg
    16921 ccggcgggtg ctcgcctggc ttcgcaccgg cgttcggggc gcgcaccggc tcgtcgaccg
    16981 cgggcggctt cacgccggtc acggccagca tcgcccgcgc cgatggcgat cagttcctgt
    17041 ctggggtcag ggtcgacatg ccacctggcc tcggagcgat cgtcaagaag atgccgaagt
    17101 gctcgagcac gctggccgcg cgcggcgcct gtcccgcgtc gacgctgatc gggacggcca
    17161 gcacgcgatc cggtgccgga gccgagccct tcccgctcag cggcccggtc tacttcaccg
    17221 agggctacaa gggtgcgccg ttcggcatgg tcgcggtgat tcgtgcgatc gccggcccct
    17281 acgacctcgg cacggtcgtc gtccgccagt cagtcttcgt cgatcccgag gacgcccaca
    17341 tcacggtcgt cagcgacccg ctgccgcaga tcctcgaggg ggtcccgatc cggctgcggg
    17401 cggtcgacgt gacgatcgac aggtcgaagt tcgtctacaa cccgacctcc tgtggcagga
    17461 agtccgttgg cacgacgctc cactcgatcc agggcgccgt cagcaggtcg agctcgagca
    17521 tcgcgacgga ccgctgtcag gcgctccgct tcaagccgcg catgtcgatg cgtctgaccg
    17581 gccgacgcca ggtcaagctc ggcaagcacc ccggcctgcg cgtccgggtg cgccaggcac
    17641 gcggccaggc gaacatcgga cgcgcacgcg tcgtgctccc gctgagcctg gcgctcgatc
    17701 ccgccaacgc ccgtgcgatc tgcggcttcg aggcggggct gaaggcccgc tgtcccgcca
    17761 agtcgaagat cggccgcgcg cgtgccgtct cgcccgcgct gaaccgcccg ctgagggggc
    17821 ctgtctactt cgtccagggc atccgcatcg atcccgagac cgggaaccgc atccggaccc
    17881 tgccgagtct gctggtcaag ctcaacggcg agatccgggt caaccttcgc ggcaccacca
    17941 gcgtggcccg ccgtcgcctc gtcagcacct tccccacggt cccggacgct cccgtctcgc
    18001 gcttcgacat gcgcctcaag ggcggcaagg gcggaatcct gaccgtcgcg acgcgcgccc
    18061 gtcgcggcat ctgcgcccgc cgccggcaga tcagccgggc cgtcttcatc gggcacaacg
    18121 gcaagcgcaa ggtccagcgc gtgaagatgg ccaagccatg caagaagcgc aaggctccta
    18181 ggaagcgccg ccgttgatac tccagtacgt ctaagcggtc agaccaacct tggccccgag
    18241 acgccgctcc taacccattc ttcggggtta cattgggtgt gagacgggcc cgatacgggc
    18301 atgtctctta gccagatggg tggactgacg cgacgacgtg gatcggagac tcgcatgcgg
    18361 tttttgggat tctgctgtgc cctgatcgga gtgctcgcgg cctcggcccc ggccgctcag
    18421 gcccagcagt tcgggatcga gaacttcacc ggaatggtgt tcgcggacga gacgcgcaac
    18481 cgcacggacg cgtacagcaa caccggcggt cgcacgactg acgcctaccc cgaccccagc
    18541 actggcggca ccggcatcta cgacctcgcc ggcggccacc cgttcatcgg cgtaaccgac
    18601 ttcagcttca accgcgtcag tccgaccgga ccgcccgccg gcgggaatgt cgaggagctg
    18661 cgggtggaca tcccgcgcgg cctgatgccc aacccgcgcg ccttcgcgcg ctgcaccgat
    18721 gctcagctcg aggcgcttgc ctgtccgatc gagtcccaga tcggctccga ggagctgcgg
    18781 ctgcacatct cggcgctggc cgcgcttggc gacatcacgg tacatgtccc gctctacaac
    18841 atgacgccgc tcagtcatcc agaggatccg gggccgcggc gggacaccgt cgcccgtttc
    18901 gccttccatc ccgccgaggc cgccgaggcg ataagcccgc ttccaccgct tccccctctc
    18961 ccgccaccgc tcgacacgtt gctgccggcc ggtctgcagg gcctcgccgc cggactcgcg
    19021 aaccttcact cagtgcacat cgtcggtggc gtgcgcgacg agccgacggc cttcggtccg
    19081 ttcgactacg ggctcttctt cacgatcgat cacctgcccg cctacaaccc cgccaatccg
    19141 gcctcgcccg gcgtggttcg caccaaccta accttttggg gagcaccggg cgatccggca
    19201 cacaaccccg cggggccggg ccagcgggga cgcggcgtca gctgcgtggc gctgggcaac
    19261 ctgccgaact tgggccagtt ctgcacgccg atcccctcgc tcgcaggcgc gcccgatccg
    19321 agcatcccct tcctcagcaa cccgaccgag tgcaccaatg cgccgctggt cggccggctg
    19381 acggtgttct cgcatccgca ggccggcggc caggtcctca gcgatacgcg gaccgaccag
    19441 acgccggcga tagtcgaccg cgacgatggg caggtcaaga acggcgccca ggagtgccag
    19501 cagctgtccc tcgcgccggc gatcgatcta aggcccggca ccgatcagcc cgacacgccc
    19561 gtcggccccg aggtcgccat ttcgatggcc aacgaggggc tggccaaccg caaccggttc
    19621 gccgagtcgc acgccaagga catctcggtg acgctgccgc cgggactgac gatcaaccca
    19681 tcggtcgcca acggcctgca ggcctgtacc gacgaccaat tcgccgcgaa cgtcggcgtg
    19741 cccgccggcg aggcctgccc ggccgcctcg cggattggcg acgtgcgggt catctcgccg
    19801 ctgctcccac cggcagccgg cgacgacccc aactcgacgc ccgtgctgac cggcagcgcc
    19861 tacgtcggtc agccactggc gaacgaccag taccgcctct tcctgacgat cgagggacgt
    19921 gaggtgtcga tccggctcaa gggatcgatc cagcccgatc cgaacaccgg tcagatcacg
    19981 gcaatcttca gggacaaccc ccagttgccg ttcgaccggc tcgccgtcga cttccgcgac
    20041 ggctcgcggg cgccgttggc gacgccgctg gactgcggtc cgaaggcggc gagcacgacg
    20101 gtcacgccat ggtcggggac cgcgcccgtc acggcgacga gcccagcctt caacatcggc
    20161 ggcgcgagct gtccagtccc cttccggacg aactttgacg tcgcgaccgc gtcgcctggc
    20221 agcgggcagt tctcgccgct cagcgtgcac atcggccgtg gcgatcgcaa ccagttcctg
    20281 tccaaggtga gggtcgacgc cccgcccggc ctcgccgcca agatcaaggg cgtcacccag
    20341 tgcgaggacg cggccgcaaa cacgggcgcc tgcccgccgg cgagccggat cggcaccgtc
    20401 acgacgacgg ccggcgcggg cagcgagccg taccggctca gcggtccggt ctacctcacc
    20461 gaggactaca agggcgcgcc gttcggcatg gtcgcggtga tccgcgcaat cgccggcccc
    20521 tacgacctcg gcacggtcgt cgtccgccag cagttgcacg tcgatcccga ggacgcgagc
    20581 gtcacggtcg tcagcgaccc gctgccgcag atcctcgagg gcgtgccgat tcgactgcgc
    20641 agcgtcgacg tcacactcga caggcgcggg ttcacctaca acccgacgtc gtgtgggacc
    20701 aaggagatcg ggggcaggat gcactcgatc aacggcactg tcggcgagca cccgaacgcg
    20761 ttcatccgct tcgccggctg cgaccggctc ggcttcacgc cgcggatgcg gatgcggctc
    20821 accggggcac gccagatgcg ccagggcggc catcccggcc tgcgggcgcg cgtgacccag
    20881 cgcgcgagcc agtcgaacat cgccagcgcg gtggtcaggc tgccgaagag cctggcgctc
    20941 gatcccgcaa acgcacaggc ggtctgcggc ttcgaggccg cacagcgggc gaagtgcccg
    21001 cgcaagacgc ggatcggcaa ggcgacggcg gtctcgccgg tgctcaaccg cccgctgcgt
    21061 ggaccggtct acttcgtcca gggcatccgc atcgatcccg agaccgggaa ccgcatccgg
    21121 accctgccga gcctgctggt caagctcaac ggcgagatcc ggatcaacct ccgtgcgacg
    21181 accgacgtcc ggcgcggtgc gctggtcagc accttcccga acgtgcccga cgcgcccgtc
    21241 tcgcgcttca gcctcagcct gaagggcggc aagggcggcg tgctggcggc gaccggacgg
    21301 ccgtcgatct gcggcaggcg tcaggtcgcg attgcccgca cggtcggcca caacggcaag
    21361 cgcgccaagc agcgccgggt gcggatgacg accaagtcgc cgtgcaggaa gctgaagcgg
    21421 attcaggcca aggcgaagaa gcgcaaggcg gcggcgcagc gcagggcgcg ccgctagtcc
    21481 tccagccgcg tcctgaaccc ctccgacggc tcgacgacgg cgcccgcatc gcgggcggcg
    21541 tcggcgagcc agttgtcgga ggtgacgaca tgcagcgtcg ccgggctggg gtgggccttg
    21601 accagctcga ggatcttgta gtcggcggcg tcgggcttcg gccgcggtgc gtgctcgatc
    21661 gctatcacgg tcgaccgcag cggcggccgc ggcgctcttt cgaagacgac cgtcacgtcg
    21721 tcgccgctgg cggccgagaa ccgctcgagc tggtcgacca gccgcgccat cgcggcgtcg
    21781 cggtccctcc accagccgtc cggcctggtc ccgatgacgt tcatgccatc gacgacgaag
    21841 tgcatgcgag taagcctacg tcttcggcgt gaactgatag ccgtacaggc ggaagccggg
    21901 ctgtggcgcc tcgaccgggc cgtcggtctg gcgcaggccc ggctcgtcga ctccggcgcc
    21961 tgccactacc gcgtaggccg ctccggcgct gaacgagcct ggccgcgcgg tcgccccagc
    22021 tgcgcgcgcc tcgaggtagt cctcgccctc gtcccactcg ccgtcgagct cgagccggtc
    22081 ttggtcggcc ggcagtgcga tgtcggcggt ctgcgcctcg agcaggacgc cctcggcgtc
    22141 ctcggggcgc accggctcca tcggctccgg cagctggagc gagccgtcga tctcgagcag
    22201 cacctcctgg atcgcgcgct cgcagtcgag gtagtcgccc tcgccgtagt ggatcgagcg
    22261 cagccgcccc tcgacgtcga acaggtagcg gccaggccag cccttgttgg cgtactcgcg
    22321 ccagacctcg aagcccggat cgagcaggac ggcgtattcg atgcccattc gctgcacggc
    22381 tcgctcgacg acgtcgcggt cggtgccgaa cgagtacccg gggcagtgca ccccgatcac
    22441 gcgcagcccc cggtctccgt accgcgcgtg ccactcgcgc aggtagggga gggtgcggtg
    22501 cgagttgatc cgcgcgacgt cgaagaactc gatcagcgtc gcctcgcggt tgacgagctt
    22561 catcatccgc aggaaggcga cgttgagcca ctccatcttg ggcgggaact cgggcgcgcg
    22621 gatgtcagac ccctcaggtg ctctcacggg gcaagcatgc cggacccgga ctaggagagg
    22681 ccggccagcg cgcgcagcag ctccggcgag acccagagct cgacggccgc cgacaccagc
    22741 agcaccggca cggcgagggc gacggtgacg aacgtcgcgg ccagcagctc gtcccagcgg
    22801 cggcggcggc tggcgatgat ccaggccgcg agcggcagga agagcgcggt cagctcggga
    22861 atggcgtggg ggagcaggcc gaggatcaac gtgccggggc cgatgcccag ctggccggcg
    22921 acgtccgagg ccaggatgcc gatcccgaac gcctgcgtcg tcagcgagaa gagggtcgcg
    22981 cagatcacga agccgatcgc cagcggcccc gccttctcgt gcacccagcg gtcgaccccg
    23041 ctgcggttgg cggccgacag cggcagcgag ctgccggcga tgaagccggc gacgcaggcc
    23101 atcgcatgca acgccagcac gagcccgttg cgatacagga tgcgtccgta gtcggcagcc
    23161 gtggccgagt cgttgacacc aggcagtcgc agcagcgtcg ggtcaggggg tatcgccttg
    23221 gcgacgaggt agaccgccag cagcagcgcg caggcgacgg cgaagctgag agcgacccaa
    23281 ggccagagga tgcgtccggg gcgctcgttc cactcgcgca gcgcgacccg cgtgtcggcc
    23341 atcccgcgca ccagcaccag gtcgttgacg ccgctcacga gacgaccatc ggtcgctcgc
    23401 cggccgatgt ttagcgtcag ccggcggcgt cggctgtcgg ctcgcgccgc agggcgttga
    23461 ccggcccgtc gacccagacc cggacgcgat cgcccgggtg ccagatcgga aagcccatcg
    23521 agcggctgcg caccttgcgt cccgacgcca gctgcacgac gacgagctgg tcgtggccgt
    23581 agtactcgcg ggcgaccacg acgccctcgt ggccgcggtc gccgcgcggc gccggcccct
    23641 gtccgagcgc gaccgactcg ggccgcacga cgacgtctac ctcgccctcc agctcggccg
    23701 ggattgggaa gcggccgagc tcgcagtcga ccgctccgcc ggccgccttg ccgggaagga
    23761 tctccgcatc gccgaggaag ctcgtgaccc actggctcgt cgggcgtccg tagacctcct
    23821 ccggcgtacc gtcctgctgg atccggccgt cgcgcatcac cgcgacccgg tcggcgaggc
    23881 tgagcgcctc ctcctggtcg tgggtgacca gcagcgccgt cgtagccgct tcgcgcagga
    23941 tcgtctcgac gtcgcgccgc aggcccgccc gcagggtggc gtcgaggctc gagaacggct
    24001 cgtcgagtag caccacctcc ggctccggcg ccagcgcccg agccagcgcg acgcgctgac
    24061 gctcgccgcc ggagagctcg tgcgggtagc ggtcggcctt ctgctccagg ccgacgagcg
    24121 ccagcgtctc gcgggtccgc tcgcggcgct cccgcgtcga ccgccgcgcc agcccgaacg
    24181 acacattggc gtcgacgcgc aggtgcggga agagggcgta gtcctggaag accatcccga
    24241 tccggcgccg ctccggcggg accgagcggc ccgcgccggc gacgacctcg ccgccgaccg
    24301 tgatcgtccc ggcatccggc gcctcaaagc cggcgatcag ccgcagcagc gtcgtcttgc
    24361 cgcagcccga cggcccgagc acggcgacca gctcgccctc ctcgagcgac aggtcgaccc
    24421 ccgcgaccgc cctcgtcgcg ccgaagcgct tctcgacggc ggcgacccgg acactcatga
    24481 cgccgccctc cgcgacagcc ggatcagcac gccgaccggg atcaggccga ccagcacgat
    24541 cagcagcgcc ggcagcgccg ccgtctcgta gagcgactcg gtcgtcgcct gccagaccgc
    24601 gatcgccagc gtgtcgccgc ccagcggccg cagcagcagc gtcgccggca gctccttcat
    24661 gacctcggtg aacacgagca gaccggcggt cagcagcgcc ggcgcgatca gcggcacgtg
    24721 gacgcgcgcg agcacgtcga ggcggtcgga gccgagcccg cgggccgcct cgtcgagtga
    24781 gcgcgggacg cgctcgaggc cggtgtcgac ggattggaag gcgagggcga ggaagcgcac
    24841 ggtgtaggcg gcgaagagtc cgatcacggt gccggtgagc agcaagccca ctccggcggc
    24901 gtcgtcgagc cggtggtcga gccagccgag cggcacgatc acggccacgg cgaccaccga
    24961 tcctggcagg ccgtagccga tcgtggagac gcgcagggcg gcggcgctgg tccgcgagct
    25021 gctggcgcgc cggccgtagg cgagcaccag ggcgacggcg acgacgatcg cgatcgacat
    25081 cccggccagc atcaggctgt tgcgcgcggc gagcgcgaac tccggcgcca gcagaccgtc
    25141 gcggatcgag gccagcgacc aggcgccaag ctgggcgacc ggcgccagca cgaccagcgc
    25201 cagcagcagc agcggcccgg cgaccgcggc caccgccgcg ggtccgtgca gccgcaccgg
    25261 cattgcgccg cgccgggttc cctgggcgta gcgcgcacgc ccgcgcgaga tccgctcgag
    25321 catcagcagc gcgagcgtga tcgtcagcag catcgcgccg agctgcagcg ccgcgtcgcg
    25381 gtcgaaggcg ccgaaccaga ccttgtagat cgcgttcgtg aacgtctgca cgccgagcag
    25441 gttgaccgtg ccgaagtcgg cgagcgcctc catcagcgcg agcgcgacgc cgccgacgat
    25501 cgccggtcgc gcgagcggca cggctatccg cagcaccgcc tgcaggcgcg tcagcccaag
    25561 cccgcgcgcc gcctcgatca tgtcgcccgc ctgggccagg aacgccgctc gggcgagcag
    25621 gtagacgtac gggtagagca cgagcgtgaa gaccgccacg gcgccggccg tcgatcggac
    25681 cgtcgtcagg ctcaggccga ccgcgtcggc ggacccgagc gcgaacagcg tgaagacgta
    25741 gccgggcatc gccagcggga gcaccagcgc ccactcgatc cagcgccggc ccgggaagtc
    25801 gcagaacacc accagcgctg ccagccccgt cccgagtgcc agcgtgccgg cgccaacgcc
    25861 ggcgaccagc agcgccgtcg ctatgagcgc gtcgggcagc aggccggcga agtccgacag
    25921 cgcgtccggc gcggccagga agctcgccgg caggacgatc agcggtcctg cgaccgcggc
    25981 cgcgacgagg acggtagcca ccgtccagcc gtcggcgggg acggcgaccc ggcgcgcggg
    26041 gg
//
LOCUS       DATABV010000174         6014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2064024, whole genome shotgun sequence.
ACCESSION   DATABV010000174 DATABV010000000
VERSION     DATABV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064024"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="VHF88_07585"
     CDS             complement(<1..1708)
                     /locus_tag="VHF88_07585"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX2161667.1"
                     /translation="MFSKVLVANRGEIAIRVMRALEELGIGSVAVYSEADRDAPHVRR
                     ADEAYLLGPGPAAESYLVVEKILEVAKQSGAEAVHPGYGFLAENAPFARACEEAGIVF
                     IGPPADAIDAMGSKTKARELMQAAGVPIVPGTTDPVETVEDARKLIDDSIGYPVAIKA
                     AGGGGGKGFRVALTEDELQDAFEGAAREGEKFFSDATVYIERYLPDPRHVEVQVLADS
                     QGNVVHLFERDCSVQRRHQKVIEEAPAPAVDPELRERIGKIGTDAAKAVGYRSAGTIE
                     GLLQDGEYYFLEMNTRVQVEHCVTEMVTGVDIVREQILVAAGNELSFRQEDVEIRGHA
                     IECRINAEDAAKNFAPAPGQIGAYREPSGPFVRVDSGAETGYAVLPLYDPMIAKLIVW
                     DVDRQASTRRMLRALSEYEIEGVKTLIPFHQALLATDEWARGETCRNLLEDKKWLKTT
                     APEQPVTPVDSDDDEEKVERDYAVEVSGRKFDVKVIGSALGAVAAANGTAAPARKAPK
                     RSERKKSGGGGPDQLESPLQGNMWKVVVKQGDVVEEGQLLCIIEAMKMENEITAHKAG
                     TIA"
     gene            complement(1729..2643)
                     /locus_tag="VHF88_07590"
     CDS             complement(1729..2643)
                     /locus_tag="VHF88_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2161668.1"
                     /translation="MDPAIVAFGLGVGVLVGMTGIGGGSLMTPLLILVFGIKPITAVG
                     TDLAYGAVTKTVGGWRHLRQGTVDMTLSMWMAVGSVPAAIGGVYVLELLEHAYGDEFD
                     ELLIVVLAVTLLFTGIMTLTRALFLKALHDKERDSVEMTTRNKVNSIVLGALVGFVLG
                     VTSAGSGALIAVGLILLFRLVPSRVVGTDIFHAAILLWAAAAAHLVAGNVDFALAGNI
                     LIGSIPGVWVGSNLTLRVGNSVLRTTLALVLIGAGLALLSKAGVGIPGLVIAAFPAAI
                     AVMVVTVLARERRDKRRAATARDVPAES"
     gene            complement(2715..3344)
                     /gene="cysC"
                     /locus_tag="VHF88_07595"
     CDS             complement(2715..3344)
                     /gene="cysC"
                     /locus_tag="VHF88_07595"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX2161669.1"
                     /translation="MSKDQPHQASEGSAISPNVRWHAVEVTRADRSRVTGGEGMTVWL
                     TGLSGSGKSTIAVAVEAALLGRGRHAYLLDGDNLRHGLNGNLGFSAEDRAENVRRVGE
                     VAKLLADAGTVTLVSLVSPYAADRERVRAVHDEAGLRFVEVFVDTPLEECERRDPKGL
                     YAKARAGELKGMTGIDDPYERPARPELTLRPTEQSVEQSVAAILAAIDR"
     gene            complement(3392..4696)
                     /locus_tag="VHF88_07600"
     CDS             complement(3392..4696)
                     /locus_tag="VHF88_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2161670.1"
                     /translation="MAGPATNGKVHVDSRPSELVRVATAGSVDDGKSTLIGRLLFDSK
                     QILQDQLEHVERVSTERGDGYVNLALLTDGLRAEREQGITIDVAYRYFHTAKRKFIIA
                     DTPGHEQYTRNMVTGASTADLALVLVDARNGVVEQSRRHAFIASLLRIPHLVVAVNKM
                     DLVDWDEAVFDRIVADFSGWAAKLDITDITFIPMSALMGDNVVDRSKNMDWYDGPSLL
                     YYLEHVHIASDRNLIDCRFPVQWVIRPMDDEHHDYRGYAGQVASGVIAVGDEVVVQPS
                     GRTTRIAGIDTQDGPLQEAYPPLSVTVRLEDDVDVSRGDMICRVNNSPTPTKEVEATV
                     CWMSERPLSPSGRYRIKHTTRTALAKVDDLRYRFDVNSMHRDESATGLALNEIGRVRL
                     RLSAPLLVDEYRRNRATGSFVLIEESTNDTVGAGMILGADED"
     gene            complement(4697..5611)
                     /gene="cysD"
                     /locus_tag="VHF88_07605"
     CDS             complement(4697..5611)
                     /gene="cysD"
                     /locus_tag="VHF88_07605"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX2161671.1"
                     /translation="MTAVSYEVSHLRALEAEAIHVMREVAAEVERAVLLFSGGKDSIV
                     LLRLAEKAFYPAKFPFAVMHVDTGHNFPEVLEFRDRRMEELGARLIVASVQEAIDAGK
                     VVEETGPRASRNRLQTVALLDAITKHDFKAAFGGARRDEERARAKERIFSFRDDFGGW
                     DPKAQRPELWNIYNAHMNPGEHMRVFPLSNWTELDVWQYIREEELEVPAIYLSHEREV
                     FERDGMLYAVSPYVELLPGEKTFTTAVRYRTVGDMSCTGAVQSTAATLDDVVAEIAAT
                     RITERGETRADDRFSEAAMEDRKREGYF"
     gene            complement(5660..>6014)
                     /locus_tag="VHF88_07610"
     CDS             complement(5660..>6014)
                     /locus_tag="VHF88_07610"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX2161672.1"
                     /translation="FWKRDPEACCGIRKVKPMREALRGCGAWVTGLRRDQSPTRANAP
                     KLGWDKKHGLWKVAPLASWTDKDVWNYIAEHDIPYNELHDRGYASIGCTPCTNAGSGR
                     EGRWAGSGRLECGLH"
BASE COUNT          873 a         2111 c         2082 g          948 t
ORIGIN      
        1 cggcgatcgt gcccgccttg tgggcggtga tctcgttctc catcttcatc gcctcgatga
       61 tgcagagcag ctggccctcc tcgaccacgt cgccctgctt gacgacgacc ttccacatgt
      121 tgccctgcag cggcgactcg agctggtcgg gcccgccgcc gccagacttc ttgcgctccg
      181 agcgcttcgg cgccttgcgc gcgggtgccg cggtcccgtt ggcggccgcg acggcgccga
      241 gcgccgagcc gatcaccttg acgtcgaact tgcgccccga cacctcgacc gcgtagtcac
      301 gctcgacctt ctcctcgtcg tcgtcggagt cgaccggcgt caccggctgc tccggcgcgg
      361 tcgtcttgag ccacttcttg tcctcgagca ggttgcggca ggtctccccg cgcgcccact
      421 cgtcggtcgc gagcagcgcc tggtggaaag ggatcagcgt cttgacgccc tcgatctcgt
      481 actccgacag cgcgcgcagc atccgccgcg tcgacgcctg gcggtcgacg tcccagacga
      541 tcagcttcgc gatcatcggg tcgtagagcg gcagcaccgc gtagccggtc tcggcgccgg
      601 agtcaacgcg cacgaacggg cccgacggct cgcggtaggc gccaatctgt cccggggccg
      661 gggcgaagtt cttggcggcg tcctcggcgt tgatccggca ctcgatcgcg tggccgcgaa
      721 tctcgacgtc ctcctgccga aacgacagct cgttgccggc tgcgacgagg atctgctcgc
      781 gcacgatgtc gacgccggtg accatctcgg tcacgcagtg ctcgacctgc acgcgcgtgt
      841 tcatctcgag gaagtagtac tcgccgtcct ggagcaggcc ctcgatcgtg ccggcggagc
      901 ggtagccgac ggccttggcg gcgtcggtgc cgatcttgcc gatccgctcg cgcagctccg
      961 ggtcgacggc gggggccggc gcctcctcga tcaccttctg gtgacggcgc tggaccgagc
     1021 agtcgcgctc gaacaggtgg acgacgttgc cctgcgagtc cgcgagcacc tggacctcga
     1081 cgtggcgggg gtcgggcagg tagcgctcga tgtagaccgt cgcgtcggag aagaacttct
     1141 cgccctcgcg cgccgcgccc tcgaaggcgt cctggagctc gtcctcggtc agcgcgaccc
     1201 ggaagccctt gccgccgccg ccgcccgccg ccttgatcgc gaccgggtag ccgatcgagt
     1261 cgtcgatcag cttgcgcgcg tcctcgacgg tctcgaccgg gtcggtggtg ccggggacga
     1321 tcggcacgcc ggcggcctgc atcagctcgc gcgccttggt cttcgagccc atcgcgtcga
     1381 tcgcgtcggc cggcggcccg atgaagacga tcccggcctc ctcgcaggcg cgcgcaaacg
     1441 gcgcgttctc cgccaggaag ccgtagccgg ggtggaccgc ctcggcgccc gactgcttcg
     1501 cgacctcgag gatcttctcg acgaccaggt agctctccgc cgccgggccg ggccccagca
     1561 ggtacgcctc gtcggcgcgc cggacgtgcg gcgcgtcgcg atcggcctcc gagtagacgg
     1621 cgaccgagcc gatgccgagc tcctcgagcg cgcgcatcac gcggatcgcg atctcgccgc
     1681 gatttgcaac caggaccttc gagaacatgc ggcgcggaaa tctacctgct agctctcagc
     1741 cgggacgtcg cgggcggtcg cggcgcggcg cttgtcgcgc cgctcgcggg ccaggacggt
     1801 cacgaccatc accgcgatcg cagccgggaa ggccgcgatc acgagccccg ggatcccgac
     1861 tcccgccttc gacagcagcg ccagtccggc accgatcagg acgagcgcga gcgttgtgcg
     1921 caggacggag ttgccgacgc gcagggtgag gttcgagccg acccagacgc cggggatcga
     1981 gccgatcagg atgttgccgg cgagcgcgaa gtcgacgttg ccggcgacga ggtgggcggc
     2041 cgcggcggcc cagagcagga tcgccgcgtg aaagatgtcg gtgccgacca cgcgcgaggg
     2101 gacgagtcgg aacagcagga tcagcccgac cgcgatcagc gcgccgctgc cggcagaggt
     2161 gacgcccaag acgaagccga cgagcgcgcc gagcacgatc gagttgacct tgttgcgcgt
     2221 cgtcatctcg accgagtcgc gctccttgtc gtgcagcgcc ttgaggaaca gcgcgcgggt
     2281 cagcgtcatg atgccggtga acagcagcgt gaccgccagc acgacgatca gcagctcgtc
     2341 gaactcgtcg ccgtaggcgt gctcgagcag ctcgagcacg tagacgccac cgatcgccgc
     2401 gggcaccgag ccgaccgcca tccacatcga cagggtcatg tcgacggttc cctggcgcag
     2461 gtgccgccag ccgccgaccg tcttggtcac cgcgccgtag gcgagatcgg tgcccaccgc
     2521 cgtgatcggc ttgatgccga agacgaggat cagcagcggc gtcatcaggg agccgccgcc
     2581 gatgccggtc atcccgacca gcacgccgac gccgagcccg aaggcgacga tcgctgggtc
     2641 catcgcgcta attctcccgc aagtcggtgc gcattaggga gataagcgta cagcgcgcgt
     2701 ctaacgacgc gcgctcagcg gtcgatcgct gccaaaatcg cggcgacaga ctgctcgacg
     2761 ctctgctcgg tcggccgcag cgtcagctcc ggtcgggccg gccgctcgta ggggtcgtcg
     2821 atgcccgtca tgcccttcag ctcgcccgca cgcgccttcg cgtacaggcc cttggggtcg
     2881 cgccgctcgc actcctcgag cggcgtgtcg acgaagacct cgacgaagcg cagcccagcc
     2941 tcgtcgtgca cggcccgtac gcgctcgcga tcggccgcat acggcgacac cagcgacacc
     3001 agcgtgaccg tccccgcgtc ggccagcagc ttcgccacct cgccaacacg gcgcacgttc
     3061 tcggcgcgat cctcagccga gaagccgagg ttgccgttca gcccatggcg caggttgtcg
     3121 ccgtcgagca gatacgcatg acgcccacgc cccagcagcg ccgcctcgac agccaccgcg
     3181 atcgtcgact tgcccgaccc cgacagtccc gtgagccaga ccgtcatccc ctcaccaccc
     3241 gtcacccgcg accgatccgc gcgcgtcacc tccaccgcat gccagcggac gttcggggag
     3301 atcgcggagc cctcagaggc ctggtggggt tgatccttgc tcataccggc cagggtcccg
     3361 ccggggggct gggggcgtga gcccccagag gtcaatcctc atcggcgccg aggatcatgc
     3421 cggcgccgac ggtgtcgttc gtgctctcct cgatcaggac gaagctgccg gtcgcgcggt
     3481 tgcggcggta ctcgtcgacc agcagcggcg ccgacaggcg cagccgcacg cggccgatct
     3541 cattgagcgc caggccggtc gcgctctcgt cgcggtgcat cgagttgaca tcgaagcggt
     3601 agcgcaggtc gtcgaccttg gcgagcgcgg tgcgggtcgt gtgcttgatc cggtagcggc
     3661 cagacgggct cagcggccgc tccgacatcc agcagaccgt tgcctcgact tccttggtcg
     3721 gcgtcgggct gttgttgacg cggcagatca tgtcgccgcg cgagacgtcg acgtcgtcct
     3781 cgagccgcac ggtcaccgac agcggcgggt aggcctcctg cagcgggccg tcctgggtgt
     3841 cgatacccgc gatccgcgtc gtgcgccccg acggctggac gacgacctcg tcgccgacgg
     3901 cgatcactcc cgaggcgacc tggccggcgt agccgcggta gtcgtggtgc tcgtcgtcca
     3961 tcggccggat cacccactgg accgggaagc ggcaatcgat caggttgcgg tccgaggcga
     4021 tgtggacgtg ctcgaggtag tagagcagcg acggcccgtc gtaccagtcc atgttcttgg
     4081 accggtcgac gacgttgtcg cccatcagcg ccgacatcgg gatgaacgtg atgtcggtga
     4141 tgtcgagctt ggccgcccag ccggagaagt cggcgacgat ccggtcgaag acggcctcgt
     4201 cccagtcgac gagatccatc ttgttgaccg cgacgacgag gtgggggatc cgcagcagcg
     4261 aggcgatgaa ggcgtgccgg cgcgactgct cgacgacgcc gttgcgcgca tcgaccagga
     4321 cgagtgcgag gtcggccgtc gaggcgcccg tgaccatgtt gcgcgtgtac tgctcgtggc
     4381 cgggcgtgtc ggcgatgatg aacttgcgct tggcggtgtg gaagtagcgg taggcgacgt
     4441 cgatcgtgat gccctgctcg cgctcggcgc gcagaccgtc ggtcagcagc gcgaggttga
     4501 cgtagccgtc gccacgctcg gtcgagacgc gctcgacgtg ctcgagctgg tcctggagga
     4561 tctgcttcga gtcgaacagc agccggccga tcagcgtcga cttgccgtcg tcgaccgagc
     4621 cggcggtcgc gacccggacc agctccgatg ggcggctgtc gacgtggacc ttgccgttgg
     4681 tggccgggcc cgccacctag aagtagccct cgcgcttgcg gtcctccatg gcggcctccg
     4741 agaagcggtc gtcggcgcgc gtctcgccgc gctcggtgat ccgcgtcgcg gcgatctcgg
     4801 cgacgacgtc gtcgagcgtc gcggctgtcg actggaccgc gccggtgcag ctcatgtcgc
     4861 cgaccgtgcg gtagcggacc gccgtcgtga aggtcttctc gccgggcagc agctcgacgt
     4921 agggcgagac ggcgtagagc atcccgtcgc gctcgaagac ctcgcgctca tgtgagaggt
     4981 agatcgcggg gacctcgagc tcctcctcgc ggatgtactg ccagacgtcg agctcggtcc
     5041 agttcgagag cgggaagacg cgcatgtgct cgcccgggtt catgtgcgcg ttgtagatgt
     5101 tccacagctc cgggcgctgg gccttggggt cccagccgcc gaagtcgtcg cggaaggaga
     5161 agatgcgctc cttcgcgcgg gcgcgctcct cgtcgcggcg agcgccgccg aaggccgcct
     5221 tgaagtcgtg cttggtgatc gcgtcgagca gcgcgacggt ctggaggcgg ttgcgcgagg
     5281 cgcgcggccc ggtctcctcg acgaccttgc cagcgtcgat cgcctcctgg acgctcgcga
     5341 cgatcagccg cgcgccgagc tcctccatcc gccggtcgcg gaactcgagc acctcgggga
     5401 agttgtggcc ggtgtcgacg tgcatcaccg cgaacgggaa cttcgccggg tagaaggcct
     5461 tctcggcgag ccgcagcagc acgatcgagt ccttgccgcc ggagaacagc agcaccgccc
     5521 gctcgacctc ggcggcgacc tcgcgcatca cgtggatcgc ctccgcctcg agggcgcgta
     5581 gatgggaaac ctcgtagctc acagcagtca tagggggaac cgcgtcatcg gggccaggtt
     5641 gcgctccatg gcgcgctcgc tagtggagtc cgcactcgag ccggccggag cccgcccaac
     5701 ggccctcgcg cccggagccc gcgttcgtgc aaggggtgca gcctatcgag gcgtaaccac
     5761 ggtcgtgcag ctcgttgtag gggatgtcgt gctcggcgat gtagttccag acgtccttgt
     5821 cggtccagct cgccagcggc gcgaccttcc agaggccgtg cttcttgtcc cagccgagct
     5881 tcggcgcgtt ggcgcgcgtc ggcgactggt cgcggcgcag gccggtcacc caggcgccgc
     5941 agccgcgcag cgcctcgcgc atcggcttga ccttgcggat cccgcagcag gcctcggggt
     6001 cgcgcttcca gaac
//
LOCUS       DATABV010000175        29390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1500878, whole genome shotgun sequence.
ACCESSION   DATABV010000175 DATABV010000000
VERSION     DATABV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29390
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500878"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VHF88_07615"
     CDS             complement(<1..291)
                     /locus_tag="VHF88_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161673.1"
                     /translation="MTGVGGTWAILRGERGRTRKLGKLLALLRPYRGRVILMFVALFV
                     ATAAALAPPYLLGRAIDDGIRAGDIDALTWITIAFLGAATLNFGATYAQTYLT"
     gene            complement(288..2093)
                     /locus_tag="VHF88_07620"
     CDS             complement(288..2093)
                     /locus_tag="VHF88_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161674.1"
                     /translation="MRVFRRLLGFLRPYRRGVIVSFVLAALAMGTGILIPALVGRTVD
                     DIDNGGGSLWPLAGAIVAAGLLRLIFSVVRRLVAGRVSLGVEFDLRNLLYRHLQSLEF
                     GFFDSQQTGQLMSRATVDLQSVRFFLGYGLIFMAQASLTIVIAATVMIAVNPLLALVA
                     LAPTPWVIWVATRYGRLNRPASQEVQQRIAELTAAAEENVSGVRVVKGFAQEHRQLRR
                     FHHTVHRVFDQSMVSTRLRAFYNPYIGFLPQLGLAAIVIVGGRQAISGTISLGDFVAF
                     FGYVVMLTGPMRMLGMSLGMAQRAVASGNRIFEVLDRAPRLTSPPGSPPLPDIDAVRA
                     HAAEPRACGLGSVELHGVTFAYEGAEPVLRDIDLAVEAGRTVAIVGATGAGKTTLVNL
                     IPRLYDPTAGRVLIDGADLREVDLPSLRREVALVSDDAFLFSASLRENIAYARPDASD
                     EAVADAARRAGLAEVVAELPDGYDTLVGERGLTLSGGQRQRVAIARALLAQPRILILD
                     DATSSVDATTERGIKEALREVMEGRTTFVIAHRLSTLALADEIVVLEGGRIAARGAHD
                     ELLEASPLYREIAERGLPDQVFLTREDEEREVAGL"
     gene            complement(2118..3341)
                     /locus_tag="VHF88_07625"
     CDS             complement(2118..3341)
                     /locus_tag="VHF88_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX2161675.1"
                     /translation="MATRDDRQALPRPLAGEAATAGAVLRVIRDAGSLTRAELARRTG
                     LARSTVAQRVDTLLAHQLVREIGASDSTGGRPPTALAFNDGSGVVLCADLGVTHSRLA
                     VCDLGGAPLAEVVEDIEISAGPEAVLSVVDELFGELLGASAREPAHVRGIGVGVPGPV
                     AFDRGEPTNPPIMPGWDGFSIPRWFGERYDAEVLVDNDVNIMALGEHWTHWRDRGHLL
                     FIKVGTGIGCGIVAKGRIHRGAKGAAGDIGHIRLAGHEEVCRCGNVGCLEAVSGGAAL
                     AQRLSEQGIEATTSRDVVRLVKAGDGMATQLVREAGRDLGEVLAGCVNFFNPEVIVIG
                     GDVGQASQQLLAGVRETVFQRSLPLATEELRVVPSRLGDGAGVIGAAIMAIESVLAPH
                     AVDHAIRSGVAASRA"
     gene            3469..4632
                     /locus_tag="VHF88_07630"
     CDS             3469..4632
                     /locus_tag="VHF88_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2161676.1"
                     /translation="MGETAQLRAGIVGTGFIGAVHARSVLLAGGRVAGVAASSPESGR
                     AAAARLRAERSFDSAEALVESDEVDVVHVCTPNHLHVPLARAALRAGKHVVCEKPLAL
                     DDGGAGQLVELSDRVGLLGAVPFVYRYYPMVRELRERLTSGRCGALRLLHGTYLQDWL
                     SQADDDNWRVDRALGGSSRAFADIGSHWCDLAEFASGQRIARLSARTATVVEQRAQAA
                     GRPAFERGDGSGPARGVDTEDVAIVHVETDGGALGSVVVSQVSPGRKNRLWIEFDAAH
                     EALAFDQENPEQLWRGRRDGVTLAQRDRDLLSDDAARLATLPAGHPQGYADCFDAFVA
                     DVYAAIAGDPEPEGLPLFADGRRAVQITEAVLASAAADGAWTDVAADLVEARP"
     gene            4629..5552
                     /locus_tag="VHF88_07635"
     CDS             4629..5552
                     /locus_tag="VHF88_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2161677.1"
                     /translation="MKLGFLTACMPDRPLEEIAAWAAAHGYEALELAAWPTIGDRPFT
                     ASHLAADAFDEAEAERVRSALESNGLTLSALAYYDNNLDPDPALRDANHAHLRACIDA
                     AAALGGVPVGTFVGRDPGRSVAENLREAERVLPPLVDYAGERGVRLMIENCVMEGWHP
                     DGYPGNLAYSPELWEWMFGLGLYLNFDPSHLLWLGIDPVEALRPHVDRVAHAHAKDAE
                     TFTAQRNRYGFFGRTSTRDEDPWDMGWWRYRIPGLGQVDFARYVDALYEGGFDGVLSV
                     EHEDPVWGGSPEKVEQGLEIAQRNLRPLVVG"
     gene            5552..7078
                     /locus_tag="VHF88_07640"
     CDS             5552..7078
                     /locus_tag="VHF88_07640"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX2161678.1"
                     /translation="MAGTAAPTLEVRGLTKEYPGVRALKGVDFDVRAGEVHCLLGPNG
                     AGKSTLIKCVSGVVEPTRGEILLHGEPLPVGDPAASLERGVATIYQELDLVEDLSVAE
                     SVYLAHEPRRGPLLDLDRMRRDTAALLARLGHEGISPRTKIGALRTAAQQVVSIARAL
                     SRDVRLLIMDEPSAILDEGEVETLFDVVRRLTADGVAVVYISHRLDEIRRIGDRVTVL
                     SDGRTVATGIAASTPTDRLVELMVGSAVEQLYPERPRATGDVALSVRGLRRLPEVRGA
                     SFEVRAGEVVGLGGLVGSGRSELLHLIYGLERPDTGEVWVEGKRLQPGRPGAAIAAGL
                     GFAPEDRKSQGLLLDWSLTKNVSLSDLGRFGRGLLDVRAERAAVREQLQALKTVPDDE
                     ERIVRELSGGNQQKVVLARWLLHHCRVLLLDEPTRGVDVATKAQLYRVITDLARAGVA
                     VLVVSSELGELVGICSRILVMREGELVFEVDGAAASERELLRHAVAPTDSTDLIEEVR
                     "
     gene            7075..8103
                     /locus_tag="VHF88_07645"
     CDS             7075..8103
                     /locus_tag="VHF88_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2161679.1"
                     /translation="MTTSPTEGAAPAVAEEPLTERRLGAFRIQEYALLIAVILLFVIG
                     AVLRPDSFPTVDNVRNMLTQASVVGVIAIGMTFVIATAGIDLSVGSVLAAAGMFGGVM
                     VDPEGSTLVFIVGAVVFATALGSVNAIAVAYGRVVPFIATLAMFSIARGIALLLNDKL
                     PVGLLELNGGSFGDPAAFSLLWFGTGRIAGIPVSVYVFLGITILGWLVLNRTRYGRYV
                     VAVGGNREAARIAGVPVRRTIMSVYVLSGFLVGIAAVLLCARLGSASPVSGTLYELDA
                     IGAVVIGGTSLAGGKARIGGTFLGVLTFALIFSLMTQLDLSTEVQQVVKGAIVLGAVL
                     LQRPEARL"
     gene            8135..9184
                     /locus_tag="VHF88_07650"
     CDS             8135..9184
                     /locus_tag="VHF88_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2161680.1"
                     /translation="MSLKRLLMVVMATAMLALGGAGCGGDDEDDGGNANGGGAPAQSA
                     DQDGGSNDGPVTIVASVPPTDHGWLGAISKNAQEAADQHDDVEFRLLEAADSDSQAQQ
                     IAQVISEKPDALVVLPQDGEALTPVARRAEEAGIPVINVDRTFSTPDAATATILGDNY
                     QIGVSAADYIAQELNCEGNVVEIQGLAGISVTEERSQGFADQLEKACPDGGIEVVAKQ
                     PGDFNPDTGLKVMENILQAQDQIDAVYTHDDDMAQGVVQAIRNSGRDDEMFLTGVGGS
                     QDAMAQIKEGGLYRATFLYNPSMAASAVNMARLIALGEGFEELVPPEVPRQVIVPAAT
                     VTQDNVEEYEQYAFN"
     gene            complement(9327..10547)
                     /locus_tag="VHF88_07655"
     CDS             complement(9327..10547)
                     /locus_tag="VHF88_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2161681.1"
                     /translation="MSMAAKLEKTGEKGIYRAHRADCAVVDPKQRCSCPYTVVWRHRK
                     KQYKKTFRTFKEAKEARGKRLAGDSRPGSRDSFEDYARDWLASYRGRTKRGRISDRTR
                     NLYRRDVERWAIPFFARFKLAEIEPPDVRAFVTHLEASGLRASTVRSVLAPVRAMLAT
                     AVEDGSLASNPAREIRVGGDSDEQGEDATEIRALTKAELRQLLAEIPDEWRPLFELLT
                     QTGLRISEAIGLQWRDVEFGEHPQLHVRRQDCRGEVGPLKTKNSRRDLPLSPVLARWL
                     WRESQGKRDTAPIFTTKAGTPLSDRNVDRRVLGPAKLAAGVPWVTFHTFRHTCASLLF
                     EDGRDVKQVSKWLGHANASFTLDVYVHLMDDGKGDAGFFDDVLGAAQGNTRATGDPQT
                     AASEGAGNVRRLAS"
     gene            complement(11202..11441)
                     /locus_tag="VHF88_07660"
     CDS             complement(11202..11441)
                     /locus_tag="VHF88_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161682.1"
                     /translation="MAAERHLEAVRDVRRDRTIPSRGSEATVAAWQGIHAAFETAINN
                     PAVDAAERRYMRELAEVSGLSVAACRLARFCEGVS"
     gene            complement(11441..11944)
                     /locus_tag="VHF88_07665"
     CDS             complement(11441..11944)
                     /locus_tag="VHF88_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161683.1"
                     /translation="MISHDEYLELCHNAKDALSDLLDETDHDLGTCERTAYKSLKRYI
                     ARHEAEADEPEDDDGWAVERARRKAWDRWPMERKRAVVLELLGDKRLTGKELAQRFRQ
                     AVAADFDVYEDQVLYIVKKMLGADLDRELEQWGSRGRYRYFRKRGLEGPIAELQRQFD
                     ATTREGA"
     gene            complement(11941..12330)
                     /locus_tag="VHF88_07670"
     CDS             complement(11941..12330)
                     /locus_tag="VHF88_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161684.1"
                     /translation="MPTGKVGPAPDVPPCDRCGREAAFRDAAGEPVCEFCPAVDYGID
                     WGRSSAYTELAVAILSACAAVGATPIRRLHELVDYAATAKAVELPLAPPDSGGLEVMA
                     HGPWLEGLTPITGRGEAIVATRRGAAQ"
     gene            12771..12962
                     /locus_tag="VHF88_07675"
     CDS             12771..12962
                     /locus_tag="VHF88_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161685.1"
                     /translation="MAIAIAVAFTGESCRKALARGEACGDLGQFVYDGAQTVIVVVLL
                     VVALAGGAILNALQRAIDV"
     gene            complement(12959..13315)
                     /locus_tag="VHF88_07680"
     CDS             complement(12959..13315)
                     /locus_tag="VHF88_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161686.1"
                     /translation="MAEEEVEFYAGDELVAYMEHYGVEPVECSRCAAPAPLLAASADR
                     LFLAARRGLEADTMRAFWLRDAMLMEWGIVKVLNPSAVLACPGCSKPAEVKRTLTELY
                     LVAAQTEYAWGSGSQN"
     gene            complement(13315..19938)
                     /locus_tag="VHF88_07685"
     CDS             complement(13315..19938)
                     /locus_tag="VHF88_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161687.1"
                     /translation="MAAGTRVGTGYVEISPDFSGFQREVERALASRFSGIGAEAGKKL
                     SDGISRSVRSRRDGFGLASALAPLVRKFEKAGDDAGRKLADRIGKGAGRARNDMFGLA
                     DALGAVERQSSKAGRSAKVFERDMFGVARAADDAGRNVRASRSQLRDWATGARGSSAA
                     ARGLGDRLRGASTRLREVTAGTRAAAGGFGGMNGAMARVNRGAQFFRNVTRLLRFPAL
                     VTGLGLATQGLAALAAGFTAAASSVGPLAGALVALPAAVATAAQAFGALKLATAGIGD
                     TIKAALDAQIKGGSQAATVMGQQTAAADRVADAEMAVADAQRQTKIAQDDLTAARKEA
                     RRELEDMALAAERSGTSEEAAVLQLRQLRRELLQTALDPESTELDIRMDENALKQAQF
                     DLKQTRIEAKRTRADYRDAADEGVKGMPAIVAARRAEAEASRAETAAQRDLQRAVEAS
                     TETMAAQGAAATNLQEEMSQLPPAAQAFVRQVIAMKPRLDELRATAASGLFPGVSTGL
                     RGLMGNFGVVKGLVGDTASAFGGLAARAGKALGGTAWGRDLKLIGDQNVRSINQMGRA
                     GGNLADAFRHILVAARPFVNWLGKGTVALTGWIKTEAEAGRKSGRLAAFFERTKQTMK
                     LLGPILKGVGGAFLNIGKAARPLGNDILRALGRAAERFRQWTGSVEGQNSLRNYFADA
                     KPAIFETGKLIAAIGKAFVELGSQPGAAPLIRQVRTELLPALTDAIGTITKHFGPAFV
                     DAVTAGIKLLQTLSHESGPLVVVAKALGGVAKALRWVVEHVPAAETLVTAFLALKAVS
                     FASTILGVQKLARLVTGLAGTFRAAGTALGVSLGSGVSAGAATAAGGSAAGGGLLGAI
                     RGKVLPLAKRAGLAGVGIALADGVIGEFGRRSRERSSDLLESIRAQAEGGKLFGFDTA
                     KLPGGAGLHLHENERAARTLLPILTQLGQKRTVLSRAEVADFAAKAKNLTLTKQMRSE
                     VDGIIASLRAGSNLKVGVDLDMDPAKLQKLDAGMQFLRSGWAASMGDITKVTRRNMAI
                     IRNTIGMNTAEGRKAAGNNLRAMATAFRVQMQGTGKWTKEAMQRHRQIIRKADLVSPT
                     KAKATEFGREWAKGMNTSTEITRRGFREMIREARKMPAPMRKVALESWLAQIREARRS
                     NTITKGEFRKLRSNALAEFSGLAIGQKRKSKQGADDVLGNVARMVTQTGKGFGVLVSN
                     ANQALVAFGADRLTYEAKKWHGGEQKKQAGGFIVPGSGTGDKVRTALPAGSFVLNRKA
                     TEAFGFQRGGLAPVALEPGERVFHPDEVRRHGPAIEAMNRAVPRFQTGGMVRVPGDPI
                     GPFSDTVNRSVAGLAADFVKKFKLNISAAFNPDGGHKSPGHNVTGTAIDIIPGPGGSW
                     DLVGDAVAAAVARGLTVYYDGSRGSTALEDHGPGNHAHIELGSAGPGASIPLFEKLAK
                     VALLGSPGPLRDMGQGALDKVRRAGDRMLAKKAMVTGAGDPFSGIGGGEAGYATRAQM
                     VAWATEALHRTGHGASPEAIEKILTLARKESGWELRSLNDWDINWQQGNPSGGLMHVT
                     LDKVGGSYERLYDPVENMMASIRYQFERYGGLITHSPYAKGGLVRMVGDSLGVGTVGL
                     GGLGSKIKGLSADVLGGRFAAGSAGLFGKGFKQYIFDLGTNDTSASAPASVLRAVDRL
                     TGDAPIHALTLNGPLAAEKNAKLQGAQGGDINLLPWAARSRGLLEPDGFHATGAGYGK
                     RASMIADSVRKAADKLKREKRTDNQRKRAGRKKDSPEVVDVSQTAIGSASKAIKQAMK
                     LVRNPNAKKRVRSKQVNKLVDRLKAQFDLPGGWRNDLAFYEAEEAKTLDRAGRADEQT
                     FDVMDAEGEPVVDAEGRQVVSLGQMDGRTAEDWTRARLKNLIEWRNKLIRARIEVMRL
                     KALVEDQIKQRQRRVDELTGSIARRFKLKGHLDDLFAWVRRPKINDKKISGALREVRQ
                     LVPREMFTRIKRAVEERNRPWLKDRFQAVTRTWRADRRERSALTGHVIPALVDRRDAL
                     GGDRGEFETKLGEIQGPGYGMELSDLFSGLPQLRQFSGSIFDAHSDLSRFSEKPPLVQ
                     RDTAPQDDRDDSERIQFLSEQLTFANQRSLLKDSQFKTLLQYRLGDGIPFAGTFHEGG
                     VVPGPKGAERMALVQAGEKITPLRESESGPFAPPIVNVHVHVDKDAGVDPNKIRSVVK
                     QEMRGAGRGARRVLGGV"
     gene            complement(19973..20167)
                     /locus_tag="VHF88_07690"
     CDS             complement(19973..20167)
                     /locus_tag="VHF88_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161688.1"
                     /translation="MAGALVQHKGIVQPGSSVARRAVLVLVVPVALPLVARRAALRAA
                     ATTTVPDLATGKVHPAAEQR"
     gene            complement(20432..21127)
                     /locus_tag="VHF88_07695"
     CDS             complement(20432..21127)
                     /locus_tag="VHF88_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161689.1"
                     /translation="MTPTATIPSADKLHTRLTECEQAVADLEQEIGVAELEGKPSPSG
                     ASGRLRKARDELERVQAAIRALEDREASEADRQAMLAAARTRLGEYRWAVERYRLSED
                     AIRAHDAAAETINRLADLGMPGRVSEMFQTLGIRYAPGMKCEPYEGRRVFRDRQDELD
                     VEAGVLRPAARKRFSRPKMGESNPYFPFGWLPGRGPLLTIERCQELRHEAERLAAEQE
                     AEIERLETQGAAA"
     gene            complement(21124..21360)
                     /locus_tag="VHF88_07700"
     CDS             complement(21124..21360)
                     /locus_tag="VHF88_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161690.1"
                     /translation="MKRHKPHKDWTVEDHLRHQQDGTEPVSDDYREAAREALEDAGLE
                     VPPELQEGGRKSPAAMTAADHAAEIRRAPRERTA"
     gene            complement(22053..22352)
                     /locus_tag="VHF88_07705"
     CDS             complement(22053..22352)
                     /locus_tag="VHF88_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2161691.1"
                     /translation="MSPATLDAFLDDLVDRLAERPDALDRLAAKLAERVGTSVPATDE
                     WLDVNGAAKYLACKPQRLYALKSAGRIPFHKDGSRLLFSKAELRAFVESGGAVRP"
     gene            complement(22349..22669)
                     /locus_tag="VHF88_07710"
     CDS             complement(22349..22669)
                     /locus_tag="VHF88_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161692.1"
                     /translation="MSALSLADLLKARADWLDVYVVPSADHDSEDVVLRIDGSYRRDA
                     TIAPRMADHFAAELQAAGINAKRAPERDQFAGCHCRVPALSPRGGRQPSVCQWCQRER
                     RAEP"
     gene            complement(22666..23844)
                     /locus_tag="VHF88_07715"
     CDS             complement(22666..23844)
                     /locus_tag="VHF88_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2161693.1"
                     /translation="MVVPRAVDWLWFPYLPLGKLSALAGQMGQAKSLATCWLHAAVTN
                     GKGINLGRSANTIMWNAEDDAADTIRPRLEAAGADLERIWIETDIILTADRLASLCDE
                     MGDVALVTVDPVQAYMPAGVNSWKGQDVRSFFEPFRHLAMERGLSILLVQHLNRRADA
                     GDALARIADSQGIPQLARSVLIWGPDPSDPEGDQGSSKALTRAKGNLARSSDSATFTI
                     DERTVSQGIKAPTLVRGDDKTISADEVVSDVETRTAGDEAVDWLRLTLENGPMDAKDI
                     LAAAREVGISERTLKRAKRPAGVVSEQSRSESGITGWHWVLKGVETPCKDDGTLGPLG
                     TLGPLPQEAKDANDAKKAKRSNDLAEGFCGCRVPAPSPRGSGPPRCATCRREIREVSA
                     "
     gene            complement(24135..25007)
                     /locus_tag="VHF88_07720"
     CDS             complement(24135..25007)
                     /locus_tag="VHF88_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161694.1"
                     /translation="MIERAVYAYAEAGDALRTIRDNRLYKKDFSSFEDYCRKRWGWSR
                     TNADRQIAAAEIHHALYDHAKVAPTGATLEPPRSERAARELAPLRDDPERLSEVWTKV
                     VEKHGPEAEAKHVRAMVREIEEPAPTPPTSTITYDDGEVVYRRDNGRDPVLERCYDLI
                     LQHPGSQLVDETGRVFVSPDGNGVVCVGKIDHETCDTPSRVPTDELTARQRQRAEKAK
                     ERLWKLVSRGRIATGPDDPRERVLSVVNIDDALTVTSTDEIAEMVEELTAALGEAQAL
                     DRELRARLRDRRAA"
     gene            complement(25941..26014)
                     /locus_tag="VHF88_07725"
     tRNA            complement(25941..26014)
                     /locus_tag="VHF88_07725"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25978..25980),aa:Met,seq:cat)
     gene            26074..26967
                     /locus_tag="VHF88_07730"
     CDS             26074..26967
                     /locus_tag="VHF88_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161695.1"
                     /translation="MSEVVAPELLMAVPNVSDGRHAELLEAIERAYAPARFLDLHADR
                     DHNRSVLTLAGRQGELADGLVSGARAVAEWIDVSVHGGVHPYVGALDVMPVVYRSQAQ
                     RGPACAELLTAAARIGAELELPVILYGELATRREHRERAWLREGGPVALARRIEHGEI
                     VPDFGPARPHPTAGVVLAAARPPLVAFNVDLATPDVAVARRIAARIRESNGGLPGIRA
                     IGLWLEQRGCAQVSTNVHDHRATPLREIVEAVRAEAPVAEAELVGLAPEAAFEGFPDD
                     VPLRGFSPGRHLIENVLAAIG"
     gene            27020..27406
                     /locus_tag="VHF88_07735"
     CDS             27020..27406
                     /locus_tag="VHF88_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161696.1"
                     /translation="MAQAKTKRRRKHRGTAAGTIERAGRTGKPQTREDAKQIARQRRA
                     ERLDKPPEWKSTINRAAIAAIVFAVLVVALFGRPIVSGLLLGAVMFAIYVPLGYATDS
                     AIYRFRTKRRSSGGSRPPRPPKASGR"
     gene            27454..28092
                     /locus_tag="VHF88_07740"
     CDS             27454..28092
                     /locus_tag="VHF88_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2161697.1"
                     /translation="MEVRMFSVGPIQENCFLFRHDGSERALIVDPGDEAERLLAAIGE
                     LGVTLDGILLTHTHFDHVGAVAPLAKATGAEVWVPEIEKPVLADINSYVPWPEFGPFE
                     SWDAEHTLTGGERLELAGFEIDVLFTPGHSPGHVSFHIPAEQAIFSGDVLFQGSVGRT
                     DLPGGDPQVLMETLKSLAETLPDETSVHPGHMGMTTIGAERATNPFLGQFAR"
     gene            28094..29335
                     /gene="hisS"
                     /locus_tag="VHF88_07745"
     CDS             28094..29335
                     /gene="hisS"
                     /locus_tag="VHF88_07745"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX2161698.1"
                     /translation="MAAKLQAPRGTFDVLPDDGQRRRELYRRAGSVLGRYGYRPFETP
                     VFEDTDLFARGVGESTDIVRKEMFTFEDRGGRSLTLRPEGTAGVARAYVEHGMHKLPQ
                     PVKLWYWGPFFRHERAQAGRYRQFAQVGVEALGSDDPSLDAEAILLLAELLEAAGVRE
                     VRLRLASLGSLDARAAYLDELRRYLREHDGELSDEVRDRIDANPLRAFDADHPGTRAV
                     MENAPRLLDSLAGDDAEHFTEVRALLDGAGVSYDVDPTLVRGLDYYTRTVFEFESSRL
                     GAQSGVGGGGRYDGLVEQLGGPATPGVGWAAGIERILLATDGELGGFPRPVFVAVADG
                     GDRRAAFALATALREAGIRSELEQAGRSLKGQLKHADRLGAKAVAIVGEGIEWKDMGT
                     GEQRAVTNQQEAVRIAVGLDA"
BASE COUNT         4244 a        10205 c        10342 g         4599 t
ORIGIN      
        1 ggtcaggtag gtctgggcat aggtcgcgcc gaagttcagc gtggcggcgc cgaggaaggc
       61 aatcgtgatc caggtcagtg cgtcgatgtc gccggcgcgg atgccgtcgt cgatcgcgcg
      121 gccgagcagg tagggcggcg ccagggcggc ggcggtcgcg acgaacagcg cgacgaacat
      181 caggatcacg cggccgcggt agggccgcag cagcgccagc agcttcccga gtttgcgcgt
      241 ccggccgcgc tcacctcgaa gaatcgccca ggttccgccc acacctgtca cagcccggcc
      301 acctcgcgct cctcgtcctc acgggtcagg aagacctggt ccggcaggcc gcgctcggcg
      361 atctcgcggt agagcgggga tgcctcgagc agctcgtcgt gggcgccgcg cgcggcgatc
      421 cggccgccct cgaggaccac gatttcgtcg gcgagggcga gcgtcgagag gcggtgggcg
      481 atcacgaaag tcgtgcgccc ctccatcacc tcgcgcagcg cctccttgat cccgcgctcg
      541 gtcgtcgcgt cgacgctcga cgtcgcgtcg tcgaggatca ggatccgcgg ttgggcgagc
      601 agcgcgcggg cgatcgcgac gcgctggcgc tggccgcccg acagcgtcag cccgcgctcg
      661 ccgacgagcg tgtcgtagcc gtcggggagc tccgcgacga cctcggcgag gccggcccgg
      721 cgggccgcgt cggcgaccgc ctcgtcgctg gcgtcggggc gcgcgtaggc gatgttctcg
      781 cgcagcgacg ccgagaacag gaatgcgtcg tcggacacca gcgccacctc gcgccgcagc
      841 gacggcagat cgacctcgcg caggtcggcg ccgtcgatca aaacccttcc ggcggttggg
      901 tcgtagaggc gcgggatcag gttgaccagc gtcgtcttgc cggcgcctgt cgcgccgacg
      961 atcgccaccg tccgtcccgc ctcgaccgcc aggtcgatgt cgcgcaggac cggctcggcg
     1021 ccctcgtagg cgaacgtgac gccatgcagc tcgaccgaac ccaggccgca agcgcgcggc
     1081 tctgccgcgt gcgcgcggac agcatcgatg tcgggaagcg gcgggctgcc gggcggtgag
     1141 gtcagccgcg gcgcgcggtc gagcacctcg aagatccggt tgccggaggc gacggcgcgc
     1201 tgggccatgc cgagcgacat gccgagcatc cgcatcggcc cggtcagcat cacgacgtag
     1261 ccgaagaagg cgacgaagtc gccgaggctg atcgtgccgc ttatcgcctg ccgcccgccg
     1321 acgatcacga tcgccgccag gccgagctgc ggcaggaagc cgatgtaggg gttgtagaag
     1381 gcgcgcaggc gggtcgagac catcgactgg tcgaacacgc ggtgcacggt gtggtggaag
     1441 cggcgcagct gccggtgctc ctgcgcgaag cccttgacga cgcgcacgcc cgagacgttc
     1501 tcctctgccg cggcggtcag ctccgcgatc cgctgctgga cctcctggga ggccggccgg
     1561 ttgagccgcc cgtaacgcgt cgcgacccag atcacccacg gcgtcggagc cagcgcgacg
     1621 agcgcgagca gggggttgac ggcgatcatc acggtcgccg cgatgacgat cgtcagcgac
     1681 gcctgcgcca tgaagatcag cccgtagccg aggaagaagc ggaccgactg caggtcgacc
     1741 gtcgcgcgcg acatcagctg cccggtctgc tggctgtcga agaagccgaa ctcgagcgac
     1801 tggaggtgcc ggtagagcag gttgcgcaga tcgaactcga cgccgagcga gacgcgtccc
     1861 gccaccaggc gacgcacgac gctgaagatc agccgcagca gcccggccgc gacgatcgcg
     1921 ccggcgagcg gccacagcga cccgccgccg ttgtcgatgt cgtcgaccgt gcgaccgacc
     1981 agcgccggga tcaggatgcc cgttcccatc gcgagggccg cgagcacgaa cgagacgatc
     2041 acgccgcggc gataggggcg gaggaagccg agcaggcggc ggaagacgcg catcggtcct
     2101 tactttacaa agtgaagcta tgcgcggctg gcggccaccc cgctgcgaat cgcgtgatcg
     2161 accgcatgcg gagcgaggac gctctcgatc gccatgatcg ccgcgccgat cacgcccgcg
     2221 ccgtcgccga gccggctggg gacgacgcgt agctcctcgg tcgcgagcgg cagcgagcgc
     2281 tggaagaccg tctcgcggac gcccgcgagc agctgctggg aggcctgacc gacgtcgccg
     2341 ccgatcacga tcacctccgg gttgaagaag ttgacgcagc cggcgagcac ctcgccgagg
     2401 tcgcggcccg cctcgcggac gagctgcgtc gccatgccgt cgccagcctt gaccagccgc
     2461 acgacgtcgc ggctcgtcgt cgcctcgatg ccctgctcgg acagccgctg ggcgagcgcc
     2521 gcgccgccgg acaccgcctc gaggcagccg acgttgccgc agcggcagac ctcctcgtga
     2581 ccggcgaggc ggatgtggcc gatgtcgccg gccgcgccct tcgcgccgcg gtggatccga
     2641 cccttggcga caatcccgca gccgatgccg gtgccgacct tgatgaacag caggtggccg
     2701 cggtcgcgcc agtgcgtcca gtgctcgccg agcgccatga tgttgacgtc gttgtcgacg
     2761 agcacctcgg cgtcgtagcg ctcgccgaac cagcgcggga tcgagaagcc gtcccagccc
     2821 ggcatgatcg gcgggttggt cggctcgccg cggtcgaagg cgacgggccc gggcacgcca
     2881 acgccgatgc cgcgcacgtg cgccggctcc ctggcgctgg cgccgagcag ctcgccgaag
     2941 agctcgtcga cgactgacag gaccgcctct gggccggccg atatctcgat gtcctcgacg
     3001 acctccgcga gcggcgcgcc gccgaggtcg cagaccgcca ggcgcgagtg cgtgacgccg
     3061 agatcggcgc agagcacgac cccggagccg tcgttgaagg ccagcgccgt cggcggtcgc
     3121 ccgccggtcg agtcgctcgc gccgatctct cggaccagct gatgcgccag cagcgtgtcg
     3181 acccgctgcg cgaccgtcga gcgtgcgagc ccggtacggc gagccagctc cgcccgggtc
     3241 aggcttcccg cgtcgcggat cacgcgcagc acggccccgg ccgtcgcggc ctcgccggcg
     3301 agcggccgtg ggagcgcctg gcggtcgtcg cgggtggcca aggcagggag taaagcacgc
     3361 cgggcaagcc cccttttgag cgtcgcaagg ctttagagta cgcagcgtgg cctttgctcc
     3421 agcatctttt gcttgacagc cggcaaaagt gtgtgcgaat gtggctctgt gggtgagact
     3481 gcccagctac gcgccggaat cgtcgggacc ggcttcatcg gagcggtcca cgcgcgttcg
     3541 gtcctgctgg cgggcgggag ggtcgccggg gtggcggcct catctccgga gagcggccgc
     3601 gccgcggccg cgaggttgcg cgccgagcgg tccttcgaca gcgccgaggc gcttgtcgag
     3661 tccgacgagg tcgacgtagt ccacgtctgc acgccgaacc acctgcacgt gccgctcgcg
     3721 cgcgcggccc tgcgcgccgg caagcacgtc gtctgcgaga agccgctcgc gttggacgac
     3781 ggcggcgccg ggcagctcgt cgagctgtcg gatcgcgtcg gcctgctcgg cgccgtgccg
     3841 ttcgtctacc gctactaccc gatggtccgc gagctgcgcg agcgcctgac cagcggtcgc
     3901 tgcggtgcgc tgcgactgct gcacggcacc tacctccagg actggctctc gcaggccgac
     3961 gacgacaact ggcgcgtcga tcgcgccctt ggcggttcgt cgcgggcgtt cgccgacatc
     4021 ggctcgcact ggtgcgacct ggccgagttc gcgtcgggcc agcggatagc gcgtctgtcg
     4081 gcgcgcaccg cgacggtcgt cgagcagcgc gcgcaggccg ccggccggcc cgcgttcgag
     4141 cgcggagacg gcagcgggcc cgcgcgcggc gtcgacaccg aggacgtcgc gatcgtccac
     4201 gtcgagaccg acggcggcgc gctcggctcg gtcgtcgtca gccaggtctc gccggggcgc
     4261 aagaaccggc tctggatcga gttcgacgcg gcccacgagg cgctggcttt cgaccaggag
     4321 aaccccgagc agctctggcg cgggcggcgt gacggcgtca cgctggccca gcgcgaccgc
     4381 gacctgctga gcgacgacgc cgcgcggctc gcgacgcttc cggccggcca cccgcagggt
     4441 tacgccgact gcttcgacgc cttcgtcgcc gacgtctacg cggcgatcgc cggcgacccg
     4501 gagcccgagg gcctgccgct cttcgccgac ggccggcgcg ccgtgcagat caccgaggcc
     4561 gtcctcgcct cggcggccgc cgacggcgcc tggaccgacg ttgccgccga cctggtcgag
     4621 gcgcggccgt gaagctcggc ttcctgacgg cctgcatgcc cgaccggccg ctcgaggaga
     4681 tcgcggcctg ggccgcggca cacggctacg aggcgctcga gctggccgcc tggccgacga
     4741 tcggcgatcg cccgttcacg gcgtcacacc tcgctgcgga cgccttcgac gaggccgagg
     4801 ccgagcgcgt gcgatcggcg ctggaatcca acggcctgac gctctcggcg ctcgcctact
     4861 acgacaacaa cctcgatccc gaccccgccc tgcgcgacgc caaccacgcc catctgcgcg
     4921 cctgcatcga cgccgcggcg gcgctcggcg gcgtgccggt cggcaccttc gtcggccgcg
     4981 atccgggccg cagcgtcgcg gagaacctgc gcgaggccga gcgggtgctg ccgccgctgg
     5041 tcgactacgc cggcgagcgc ggcgtgcggc tgatgatcga gaactgcgtg atggagggct
     5101 ggcaccccga cggctacccc ggcaacctcg cctactcgcc cgagctctgg gagtggatgt
     5161 tcgggctcgg gctctacctc aacttcgacc cgtcgcacct gctctggctc ggcatcgacc
     5221 cggtcgaggc gctgaggccg catgtcgatc gcgtcgcgca cgcccacgcc aaggacgccg
     5281 agaccttcac ggcccaacgc aaccgctacg ggttcttcgg gcgcacctcg acgcgcgacg
     5341 aggacccgtg ggacatgggc tggtggcgct accgcatccc cggcctcggc caggtcgact
     5401 tcgcacgcta cgtcgacgcg ctctacgagg gaggcttcga cggcgtcctg tcggtcgagc
     5461 acgaggatcc cgtctggggc ggcagcccgg agaaggtcga gcaggggctc gagatcgccc
     5521 agcgcaacct gcgaccgctg gtggtcggct gatggccggg accgccgccc cgacgctcga
     5581 ggtccgcggc ctgaccaagg agtaccccgg ggtgcgggcg ctcaagggcg tcgacttcga
     5641 cgtgcgggcg ggcgaggtgc actgcctgct cggcccgaac ggcgccggca agtcgaccct
     5701 gatcaagtgc gtctccggcg tcgtcgagcc gacccgcggg gagatcctgc tgcacggcga
     5761 gccgctgcct gtcggggatc ccgcggcgtc gctcgagcgc ggcgtcgcga cgatctacca
     5821 ggagctcgat ctggtcgagg acctcagcgt cgccgagagc gtctacctgg cccacgagcc
     5881 acgccgcggg ccgctgctcg acctcgatcg gatgcgccgc gacaccgcag cgctgctcgc
     5941 ccgcctcggc cacgagggga tctcgccgcg cacgaagatc ggcgcgctgc gcaccgccgc
     6001 ccagcaggtc gtctcgatcg cgcgcgcgct gtcgcgcgac gtgcggctgc tgatcatgga
     6061 cgagccgtcg gcgatcctcg acgagggcga ggtcgagacg ctgttcgacg tcgtgcgccg
     6121 gctgaccgcc gacggcgtcg ccgtcgtcta catcagccat cggctcgacg agatcaggcg
     6181 catcggcgac cgcgtgacgg tgctcagcga cggtcgcacc gtcgcgaccg gcatcgcggc
     6241 gtcgacaccg accgaccggc tggtcgagct gatggtcggc agcgcggtcg agcagctcta
     6301 tccggagcgc ccgcgcgcga cgggcgacgt cgctctgtcg gtgcgcggcc tgcgccggct
     6361 gccggaggtg cggggcgcga gcttcgaggt tcgcgccggc gaggtggtcg gcctcggcgg
     6421 actggtcggc tcgggccgca gcgagctgct gcacctgatc tacgggctcg agcggcccga
     6481 caccggcgag gtctgggtcg agggcaagcg actccagccc ggccgccccg gcgcggcgat
     6541 cgcggcgggg ctcggcttcg cacccgagga ccgcaagtcg cagggcctgc tgctcgactg
     6601 gagcctgacc aagaacgtca gcctctcgga cctcggccgc ttcgggcgcg gcctgctcga
     6661 cgtccgcgcc gagcgcgccg ccgtgcgcga gcagctgcag gcgctgaaga cggtgcccga
     6721 cgatgaggag cggatcgtgc gcgagctctc gggcggcaac cagcagaagg tcgtgctcgc
     6781 gcgctggctg ctgcaccact gccgcgtgct gctgctcgac gagccgacgc gcggcgtcga
     6841 cgtcgcgacc aaggcacagc tctaccgcgt gatcaccgac ctcgcgcgcg ccggcgtggc
     6901 ggtgctcgtc gtctcgtccg agctcggcga gctggtcggg atctgcagcc ggatcctcgt
     6961 gatgcgcgag ggagagctcg tcttcgaggt cgacggcgcc gcggcgagcg agcgcgagct
     7021 gttgcgccac gccgtcgcgc cgaccgattc caccgacctg atcgaggagg tccgatgacc
     7081 acgtcgccga ccgagggagc cgcgccggcc gtcgccgagg agccgctgac cgagcggcgg
     7141 ctgggcgcct tccggatcca ggagtacgcg ctgctgatcg ccgtgatcct gctgttcgtg
     7201 atcggcgccg tcctgcggcc cgacagcttc ccgaccgtcg acaacgtgcg taacatgctg
     7261 acccaggcga gcgtcgtcgg cgtgatcgcg atcggcatga cgttcgtcat cgcgaccgcc
     7321 gggatcgacc tgtcggtcgg ctcggtgctc gccgcggccg gcatgttcgg cggggtgatg
     7381 gtcgatccgg agggctcgac gctcgtcttc atcgtcggcg cggtggtctt cgcgacggcg
     7441 ctcgggtcgg tcaacgcgat cgccgtcgca tacggacggg tggtgccgtt catcgcgacg
     7501 cttgcgatgt tctcgatcgc gcgtgggatc gcgctgctgc tcaacgacaa gctgcccgtc
     7561 gggctgctcg agctcaacgg cgggtcgttc ggcgacccgg cggcgttctc gctgctctgg
     7621 ttcggaaccg ggcggatcgc tgggatcccg gtctcggtct acgtattcct cgggatcacg
     7681 atcctcggct ggctcgtcct caaccgcacg cgctacggcc gctacgtcgt cgccgtcggc
     7741 ggcaaccgcg aggcggcgcg gatcgcgggc gtgccggtac ggcggacgat catgagcgtc
     7801 tacgtcctct cgggcttcct cgtcgggatc gccgcggtgc tgctctgcgc gcgcctcgga
     7861 agcgcttcgc cggtcagcgg cacgctctac gagctcgacg cgatcggcgc cgtcgtgatc
     7921 ggcggcacga gcctcgccgg cggcaaggcg cgtatcggcg gcacgttcct cggagtcctg
     7981 accttcgcgc tgatcttcag cttgatgacc cagctcgacc tctcgaccga ggtccagcaa
     8041 gtcgtgaagg gggcgatcgt ccttggggcg gtgctgctcc agaggccgga ggcgcggctg
     8101 tgagagatgt caacaaagga agaggggtgg acccatgtcg ctcaagcgac tactgatggt
     8161 ggtgatggcc accgccatgc tggcgctagg cggcgccggg tgcggtgggg acgacgagga
     8221 cgacggcggc aacgccaacg gtggaggcgc gccggcccag agcgccgacc aggacggcgg
     8281 ctcgaacgac ggaccggtga cgatcgtcgc cagcgtgccg ccgaccgacc acggctggct
     8341 cggcgcgatc tcgaagaacg cgcaggaggc ggcggaccag cacgacgacg tggagttccg
     8401 gctgctcgag gcggccgact ccgattcgca ggcgcagcag atcgcgcagg tgatctcgga
     8461 gaagccggac gcgctcgtcg tgctgccgca ggacggcgag gcgctgacgc cggtcgcccg
     8521 cagggctgag gaggcgggga tcccggtgat caacgtcgat cggacgttct ctacgccgga
     8581 tgcggcgacg gcgacgatcc tcggcgacaa ctaccagatc ggcgtctccg cggccgacta
     8641 catcgcccag gagctgaatt gcgagggcaa cgttgtggag atccagggcc tggccggcat
     8701 ctcggtcacc gaggagcgca gccagggctt cgccgatcag ctcgagaagg cctgcccgga
     8761 cggcggcatc gaggtcgttg ccaagcagcc gggcgacttc aatcccgaca ccggtctgaa
     8821 ggtgatggag aacatcctcc aggcccagga ccagatcgac gccgtctaca cccatgacga
     8881 cgacatggcc cagggcgtcg tccaggcgat tcgcaactcc ggccgcgacg acgagatgtt
     8941 cctgaccggc gtcggcggct cgcaggacgc gatggcccag atcaaggagg gcggtctcta
     9001 ccgggcgacc ttcctctaca acccgagcat ggcggcctcg gccgtcaaca tggcgcgcct
     9061 gatcgcgctc ggcgagggct tcgaggagct cgtcccgccg gaggtcccgc gccaggtgat
     9121 cgtcccggcg gcgaccgtca cccaggacaa cgtcgaggag tacgagcagt acgcgttcaa
     9181 ctaggacggc ccgtccaccc gccgggcgct atccgcgccc ggcggaacgg ccgcgatagc
     9241 tccgggtgga ctcgaaccac cgacctcacg attatgagtc gtggagggag cttgcggctg
     9301 tttgcggctg gtccctggat tcggctctag gacgccagcc tacggacgtt cccggcaccc
     9361 tcgcttgcgg ctgtctgcgg gtcgcctgtt gccctggtgt tgccctgagc ggccccgaga
     9421 acgtcgtcaa agaacccggc gtcgcccttg ccgtcgtcca tcagatgcac gtacacgtcg
     9481 agggtgaagc tggcgttcgc gtggcccagc cacttcgata cctgcttcac gtcgcggcca
     9541 tcctcgaaca ggagggacgc gcaggtatgg cggaatgtgt ggaaggtgac ccacgggact
     9601 ccggccgcca gcttcgccgg cccgagcacg cgccggtcga cgttgcgatc cgagagcggc
     9661 gtgcccgcct tcgtcgtgaa gatcggggcc gtgtcgcgct tgccctgcga ctcccgccag
     9721 agccagcgag ccagcacggg ggagaggggg agatcccggc gcgagttctt tgtcttcagc
     9781 ggcccgacct cgccgcggca gtcctgacgg cggacgtgca gctgtgggtg ctcgccgaac
     9841 tccacgtcgc gccattgcag gccgatcgcc tcagagatcc tgagcccagt ctgcgtcaac
     9901 agctcgaaga gcggacgcca ctcgtcgggg atctcggcga gcagttggcg cagctcggcc
     9961 ttggtcagcg cgcggatctc ggtcgcgtcc tcgccctgct cgtcgctgtc gccgcccacg
    10021 cggatctcac gggccgggtt ggacgcgagc gagccgtcct ctacagcggt cgcgagcatg
    10081 gcccgcacgg gcgccaggac gctgcgtacg gtcgacgctc gcagccccga agcctcgagg
    10141 tgcgtcacga aggcgcggac gtctggcggc tcgatctcgg cgagcttgaa gcgggcgaaa
    10201 aacgggatcg cccagcgctc gacatcgcgg cggtaaaggt tgcgggtacg gtcggagatc
    10261 cggcctcgct tcgtccggcc gcggtagctc gccagccagt cccgggcgta gtcctcgaag
    10321 ctgtcccgtg agcccggccg gctgtcaccc gccagacgct tgccgcgcgc ctccttcgct
    10381 tccttgaagg tgcggaacgt cttcttgtac tgcttctttc gatgccgcca aacaacggtg
    10441 taggggcagc tgcagcgctg cttcgggtcg acgaccgcgc agtccgcacg gtgcgctctg
    10501 tagatcccct tctcgccggt cttctcgagc tttgcggcca tgctcatgcc ctcctgatat
    10561 tggcagtcgg ggtggacgac gctcgaccca tagccccatc ggtcggcagg cgtccaacct
    10621 tggccagcgc cgccgcccga ataccggcgt cccacgagcc gaacaggcgc tcgaccacgg
    10681 acgcggacgg gagatcgtcg cgccgcctaa cgtcaccgtg tgtcggccag aggccggtgg
    10741 ccgcctcaaa cgcgagcagc gcatcgagaa cagcgttgcg tggccagcgc cgcggcgacg
    10801 gctgagcgtg gtggtggaag accgcaacgg cgtagggcga gacggcgcga tcaacgtgac
    10861 atgccgtctc gttgatccga gcgcgtggcc gcccgcactt gaggcaatcg tcgccgtcgc
    10921 ggatcggccg gtcgcaccta cagcggaggg ctgcagcgga gttcatgccg cccccggctg
    10981 gtcaggaacc gcgccgagac accgcgtgcc cgcgaggtgg cgtgcgttgc ggcggcagcg
    11041 ggtgaacagg gcgcgatcgt catccgagag cccgtcgatt gccaatgcct cgtcgaagct
    11101 cgcagcgatg agcttccacg cttgcctgcc ggctgcctgg gggtcggttt ctgcaattgc
    11161 ggacacggcc cgtaggcggg gctcactcgg cctggcgggc attaggacac cccctcgcag
    11221 aagcgggcga ggcgacacgc cgcgacgctc agccccgaga cctcggccag ctcgcgcata
    11281 tagcgccgct cggcggcgtc tacggccgga ttattgatcg ctgtctcgaa ggcggcgtgg
    11341 atgccctgcc acgccgcgac tgtcgcctca gagccgcgtg acgggatggt ccggtccctg
    11401 cgtacgtccc gtacggcctc aaggtggcgc tcggcggcca tcacgccccc tctcgggttg
    11461 tcgcgtcgaa ctgacgctga agttcggcga tcggcccctc aaggccacgc ttgcggaagt
    11521 agcggtagcg gcccctgctg ccccactgct ccaactcccg gtcgaggtcg gcgccgagca
    11581 tcttcttgac gatgtagagg acttggtcct cgtagacatc gaagtcggcc gcgaccgcct
    11641 gtcggaagcg ctgcgccagt tctttgccgg tcagtcgctt gtcgcctagc agctctagga
    11701 cgactgcccg cttgcgctcc atcggccagc ggtcccacgc cttcctgcgg gcgcgctcga
    11761 cagcccaccc atcatcgtcc tcgggctcat ccgcttcggc ctcatgccga gcgatgtacc
    11821 gcttgaggct cttgtacgcc gtccgctcgc aggttccgag gtcgtgatcc gtctcgtcca
    11881 ggagatcgct caacgcatcc ttggcgttgt ggcacagctc gaggtactcg tcgtggctaa
    11941 tcactgggca gcccctcgcc gtgtcgcgac gatcgcctcg cccctcccgg tgatcggcgt
    12001 gagaccctct agccagggtc cgtgggccat gacctcgagc ccgccgctgt cagggggcgc
    12061 gagcggcagc tcgaccgcct tggctgtcgc ggcgtagtcg acgagctcgt gcagtcggcg
    12121 gatcggcgtc gcgccgactg cggcgcaggc cgagaggatg gcgacggcca gctccgtgta
    12181 agccgacgag cggccccagt cgatcccgta gtcgacggct gggcagaact cgcagacggg
    12241 ttcgccagcg gcgtccctga acgccgcctc gcggccgcat cggtcgcatg gcggtacgtc
    12301 gggtgctggc cctaccttgc ctgtgggcat ggaagctaat cccttccgtg tcaagggccg
    12361 acgctggtgt tcagagcact gcgtcggccc gctttttcgt ggcggacggc aactgtagtt
    12421 gccgcccgct ggcaacgcaa gcggaccgaa cggtccgccg tctcgctcgc gtccgacaga
    12481 gccgagaaca tcccccgccg ggatggccga cgcagtagac aaagcgctcc gcgcgatcga
    12541 cgccgcaggc ggccttgtga gcgcagccga ggtagccgcc gagtggggcg tcactcgacc
    12601 caccgtctcc aagtggatcg ccgccggccg attcccgaag ccggtaaaga cggtaggtcg
    12661 agtgcggctc tacttgcgcg cccagctcga gcctctccga ccgtagcccc agacgcgctg
    12721 ttaccctgcc gcccatgcgc cggtggggga cgtggtgggc actgggatgc ttggcgatcg
    12781 cgatcgccgt cgccttcacc ggcgagtctt gccgcaaagc cctcgcgcgc ggagaggcgt
    12841 gtggcgatct gggtcagttc gtgtatgacg gtgcgcagac cgtgatcgtc gtggtgctcc
    12901 tagtagtcgc gctagcgggc ggagccatac tcaacgcact tcagcgcgct atcgacgtct
    12961 agttctgcga accagacccc caggcgtact cggtctgggc ggcgacaagg tacagctcgg
    13021 tcaaggtgcg tttaacctcg gctggcttgg agcaaccagg acacgcgagc acggccgacg
    13081 ggttgaggac cttgacaatt ccccattcca tgagcatcgc gtcacgaagc caaaaggcac
    13141 gcatcgtgtc ggcctcgaga cctcgcctcg cagcgaggaa gagccgatcc gccgacgctg
    13201 caagcaaagg cgccggggcg gcgcagcgtg agcactccac cggctcgacc ccgtagtgct
    13261 ccatgtacgc gacgagctcg tcgccagcat agaactcgac ctcctcctca gccactacac
    13321 accccctagg actcgtcggg ctccgcgacc agcgccgcgc atctcctgct ttacgacgct
    13381 gcggatcttg ttcgggtcca cgccggcatc cttgtcgacg tggacgtgaa cgttgacgat
    13441 cggcggcgca aacggtccgc tctcgctctc ccgcaacggg gtgatcttct cgccggcctg
    13501 cacgagcgcc atgcgctcgg cgcctttggg gcccgggacg acgccgccct cgtggaaggt
    13561 gccggcgaac gggatgccgt ctccgaggcg gtactgaagc agcgtcttga actgggagtc
    13621 cttgagcaag gaccgctggt tagcgaacgt gagctgctcc gagaggaact ggatgcgctc
    13681 actgtcgtct ctgtcgtcct ggggggcggt gtcgcgctgc acgagcggcg gcttctcgct
    13741 gaagcggctg aggtcggagt gggcgtcgaa gatggagccg gagaactggc gcaactgcgg
    13801 caaccccgag aacaggtccg agagctccat gccgtagccg ggcccttgga tctcgccgag
    13861 cttggtctca aactcgccgc gatcaccgcc tagcgcatca cgccgatcga ccagagcggg
    13921 gatgacgtgc ccggtcagtg cggagcgctc tcggcgatcg gctcgccagg tacgagtgac
    13981 cgcttggaac cgatccttca gccaaggtcg gttgcgctcc tcaaccgcac gtttgatgcg
    14041 agtgaacatc tcccgtggga cgagctggcg aacctcgcgc aacgcccccg agatcttctt
    14101 gtcgttgatc ttcgggcgcc ggacccacgc aaataggtcg tcgaggtggc ccttgagctt
    14161 aaaccggcga gcgatgctgc cagtcagctc gtccactcgc cgttggcgct gcttgatctg
    14221 gtcctcgacg agcgccttca ggcgcatcac ctcgatccgc gcccggatca gcttgttgcg
    14281 ccactcgatg aggttcttaa gccgcgcacg ggtccagtcc tcggcggtgc ggccgtccat
    14341 ttggccaagc gacaccacct ggcggccctc cgcgtcgacc accggctccc cctcggcatc
    14401 catgacgtcg aacgtctgct cgtcggcccg gccggcgcgg tcaagcgtct tggcctcctc
    14461 ggcctcgtag aacgcgagat cgttacgcca cccccctggg agatcgaact gcgccttgag
    14521 ccggtcgacg agcttgttta cctgcttgga ccgaacccgc ttcttagcgt ttgggttgcg
    14581 gacgagcttc atcgcttgct tgatcgcctt gctcgccgac ccaatcgcgg tctgagacac
    14641 gtcaacgacc tcgggcgagt ccttcttgcg gccagcgcgc ttgcgctggt tgtcggtccg
    14701 cttctcccgc ttgagcttgt cggcggcctt gcgaaccgag tcggcaatca tcgacgcacg
    14761 cttgccgtac ccggccccgg tggcgtggaa gccgtcgggc tcgagcaacc ctcggctgcg
    14821 ggccgcccag ggcaggaggt tgatgtcgcc gccctgggcg ccttgcagct tcgcgttctt
    14881 ctcggcggcg agcgggccgt tgagcgtcag cgcatggatg ggcgcgtcgc cggtgagccg
    14941 gtcgacggcc ctgagcacgg acgccggagc gctcgccgac gtgtcgttgg tgccgaggtc
    15001 gaagatgtac tgcttgaagc ccttgccaaa cagcccggcg ctgccggccg cgaagcggcc
    15061 cccgagtacg tcggcgctga ggcccttgat cttcgaccct aggcccccaa ggcccaccgt
    15121 cccgaccccc agggagtcgc ccaccatgcg gaccaggccg cccttggcgt acggcgagtg
    15181 ggtgatgagg ccgccgtacc gctcgaactg gtagcggatc gacgccatca tgttctcgac
    15241 ggggtcgtag agacgctcgt agctgcctcc gaccttgtcg agcgtgacgt gcatcaagcc
    15301 accagacggg tttccctgct gccagttgat gtcccagtcg ttgagagacc gaagctccca
    15361 gccggactcc ttgcgggcta gcgtgaggat cttctcgatg gcctctggtg acgcaccgtg
    15421 tccagtgcgg tgaagcgcct ccgtcgccca agcgaccatc tgcgctcgag tcgcgtagcc
    15481 cgcctcaccg ccaccgatgc cgctgaaggg atcacccgcg ccggtgacca tcgccttctt
    15541 ggcgagcatc cgatcgccgg cacggcggac cttgtcgagc gcgccctgcc ccatatcccg
    15601 aagcgggcca ggcgagccga gaagcgcgac cttggcgagc ttctcgaaca gcggtatcga
    15661 cgctccgggg ccagcgcttc cgagctcgat gtgggcatgg ttacccggcc cgtggtcctc
    15721 gagggctgtc gagccgcgcg agccgtcgta gtagacggtc aggccccgcg cgacggccgc
    15781 agcaacagca tcaccgacga gatcccagct gccgcccggc ccggggatga tgtcgatggc
    15841 ggtccccgta acgttgtgac cgggcgactt gtggccgccg tcagggttga aggccgccga
    15901 gatgttcaac ttgaacttct tgacgaagtc ggcggcgagg cccgcgaccg accggttgac
    15961 cgtgtccgag aaggggccga tcgggtctcc cggaacgcgc accatgccgc ccgtctggaa
    16021 gcgcggcaca gcacggttca tcgcctcgat cgctggcccg tggcggcgga cctcgtcagg
    16081 gtggaagacg cgctcgcccg gctcgagcgc gacgggcgcg agaccaccgc gctggaagcc
    16141 gaacgcctcg gtcgccttgc ggttgaggac gaacgacccg gcgggcaacg cagtgcggac
    16201 cttgtcgcca gtgccactgc cgggaacgat gaacccgccc gcctgcttct tctgctcgcc
    16261 gccgtgccac ttctttgcct cgtaggtgag acgatcggcg ccgaaggcca cgagcgcctg
    16321 gttggcgttc gagacaagca ccccgaagcc cttgccggtc tgggtgacca ttcgggcgac
    16381 gttgccgagc acgtcatcgg caccctgctt cgacttccgt ttctgaccga tggcgaggcc
    16441 ggagaactcg gcgagcgcgt tcgatcgcag cttgcggaac tcgcccttcg tgatcgtgtt
    16501 cgagcggcga gcctctctga tctgcgcgag ccatgactcg agagcgacct tgcgcatcgg
    16561 cgccggcatc ttccgggcct cacgaatcat ctcacggaag cccctccggg tgatctccgt
    16621 cgaggtgttc atgcccttcg cccactcgcg gccgaactcg gtggccttgg ccttggtcgg
    16681 gctgacgaga tccgccttgc ggatgatctg acgatgccgc tgcatcgcct ccttcgtcca
    16741 cttgccggtc ccctgcatct gaacacggaa ggcggtcgcc atcgctcgca ggttgttgcc
    16801 ggcggccttg cggccctcgg cggtgttcat cccgatggtg ttgcggatga tcgccatgtt
    16861 tcgacgggtg acctttgtga tgtcacccat cgacgcggcc cagcccgagc gcaggaactg
    16921 catgccggcg tcgagcttct gaagcttcgc cgggtccatg tcgaggtcga cgccaacctt
    16981 gaggttggag ccggccctga gcgaggcgat gatgccatcc acttcgctgc gcatctgctt
    17041 cgtcagggtc aagttcttgg ccttggcagc aaagtccgcc acctcggccc tgctcaggac
    17101 cgtgcgcttc tggccaagct gggtgaggat cggcaggaga gtccgcgccg cacgctcgtt
    17161 ctcgtgaagg tgcagccccg cgccgcccgg aagcttcgcc gtgtcgaagc cgaacagctt
    17221 gccgccctca gcctgggccc ggatcgactc gagcaggtcc gaagatcgct cccgcgagcg
    17281 tcgaccgaac tcgccgatga cgccgtctgc cagggcgata ccgacaccgg caaggcccgc
    17341 tcgcttggcg agcggaagaa ccttgccacg gatcgccccg agcaacccgc cccctgctgc
    17401 cgatccacct gcagcggtag ctgcgcccgc ggacactcct gatccaagtg agacgccgag
    17461 agccgtcccc gcagcacgga acgtacctgc gaggcccgtg acgagccgtg cgagcttctg
    17521 gacgccgagg atggtcgacg cgaaagacac cgccttgagg gccaggaacg ccgtcacgag
    17581 cgtctctgcg gcgggcacgt gctcaacgac ccagcggagc gctttcgcca cgccaccgag
    17641 cgccttggct acgaccacca gtgggccgga ctcgtgagac agcgtctgca agagcttgat
    17701 gcccgccgtc acggcgtcaa cgaacgcggg accgaagtgc ttggtgatcg tcccgatggc
    17761 gtcggtgagg gctggcagta gctccgtccg cacctggcgg ataagcgggg ccgcaccggg
    17821 ctgactgccc agctcgacaa acgcttttcc gatcgcggcg atgagcttgc cggtctcgaa
    17881 gatggctggc ttagcgtcgg cgaagtagtt gcggagggag ttctgtccct cgacggagcc
    17941 ggtccactgc cggaagcgct ctgccgctcg gccgagcgcg cggaggatgt cattgccgag
    18001 cggccgtgca gccttcccga tgttcagaaa cgcgccgccg acgcctttga ggatcgggcc
    18061 gagcagcttc atggtctgct tggtgcgctc gaagaacgcg gcgaggcgac ccgacttccg
    18121 tccggcttcg gcctcggtct tgatccatcc ggtgagggcg acggtgccct tgccgagcca
    18181 gttcacgaac ggccgggcgg cgacgaggat gtggcggaag gcgtcggcga ggtttccgcc
    18241 ggcgcggccc atctggttga tggaccggac gttctggtcg ccgatgagct tgaggtcgcg
    18301 gccccaggcg gtgccgccga gcgccttgcc agcccgggcg gccagtccgc cgaacgcgct
    18361 ggccgtgtcg ccgacgaggc ccttgacgac gccgaaattg cccatcaggc cacggagccc
    18421 agtcgacacg ccggggaata ggccggatgc tgcggtggcc cgcagctcgt cgagcctcgg
    18481 cttcatcgcg atgacctggc ggacgaacgc ctgggcggcc ggcggaagtt gcgacatttc
    18541 ttcctgcaag ttggtcgccg ctgcgccctg cgcggccatc gtctctgtgg acgcctcaac
    18601 ggcacgttgc aggtcgcgct gcgcggctgt ctcggcacgc gacgcctcgg cctctgcgcg
    18661 gcgggcggcg acgatcgccg gcatgccctt gacgccctcg tcggcggcgt cgcggtagtc
    18721 ggcgcgggtg cgcttggcct cgatgcgggt ctgcttgagg tcgaactgcg cctgcttgag
    18781 cgcgttctcg tccatccgga tgtcgagctc ggtcgactcc gggtcaagcg ccgtctgaag
    18841 cagctcgcgt cgaagctggc ggagctgcag gactgccgcc tcctccgacg tgccagaacg
    18901 ctcggcggcg agggccatgt cctcgagctc gcggcgcgcc tccttgcggg cggcggtcag
    18961 gtcgtcctgg gcgatcttgg tctggcgctg cgcgtcagca actgccatct cggcgtcggc
    19021 cacccggtct gcggcagcgg tctgctggcc cataaccgtg gcggcctgtg acccgccctt
    19081 gatttgggcg tcaagcgcgg ccttgatcgt gtcgccgatc cctgctgtgg cgagcttgag
    19141 cgccccgaac gcctgggctg cggtcgcgac agctgcgggg agcgcgacga gcgcgccagc
    19201 gagcggccca accgaagacg cggcagcagt gaagcccgcg gcgagcgctg cgagaccctg
    19261 cgttgccagc ccgagcccgg tgacgagtgc cgggaaccgg aggaggcggg tgacgttgcg
    19321 gaagaactgg gcaccgcggt tgacgcgcgc catggcgccg ttcatgccgc cgaacccgcc
    19381 ggcggcagcc cgcgtccctg cggttacctc gcgcaggcgc gtactggcgc cccggaggcg
    19441 gtcgccgagg ccgcgagcag cggcagacga gccgcgggcg cctgtcgccc agtcgcggag
    19501 ctgtgaacgg ctagcgcgaa cgttccggcc ggcgtcgtct gcggcgcgcg cgacgccgaa
    19561 catgtcccgc tcaaagacct tggcggaccg gcccgccttg ctcgactgtc gctcgaccgc
    19621 gccgagcgcg tcggcaagtc cgaacatgtc gttgcgcgcg cggccggccc ctttgccaat
    19681 ccggtcggcg agcttgcggc ccgcgtcatc gccggccttc tcgaacttgc ggaccagcgg
    19741 ggcgagcgcg gacgcgagtc cgaacccgtc ccgccgggac cgcacgctgc gactgatgcc
    19801 gtcggagagc ttcttgccgg cctcggcgcc gatgccgctg aaccgggagg cgagagcacg
    19861 ctcgacctcg cgctggaagc cactgaaatc tggggagatt tcgacgtagc cggttccgac
    19921 acgggtaccg gccgccacta cgccgccctc gcgatgcggt cgtaggtggt ccctagcgct
    19981 gctcggcggc ggggtggacc ttccccgtcg ctagatccgg aaccgtcgtc gtcgctgccg
    20041 cccgcagagc ggctctgcga gcgacgagcg gcagggcgac cggcaccacg agcacgagca
    20101 cggcgcggcg tgccacggac gacccaggct gcacgattcc cttgtgctgc acaagcgcgc
    20161 cggccacgag gacgaggctc gaccatgtag gacacgcatc cgttagagcc gacgcggcgg
    20221 gtgaccttcg gcggctgtcc atcgccacag acggtcagga agtccatgat ctgctgggcg
    20281 tccgcaaggc ttcgtgcggg cggctcacag ccgtagagac ctaccgagcg gcccgcaggc
    20341 ttggcgccct ccgggccacc gtatgtcgag cttggtcgca gcatcgccgg tgcctacagg
    20401 ctaactccgg gcccggaggg aaccgcagcc atcaggccgc cgctccctgc gtctcgagcc
    20461 gctcgatctc ggcctcctgc tctgccgcca accgctcggc ctcatgccgc aactcctggc
    20521 atcgctcgat cgtgagcagg gggccacgac ccggaagcca accgaacggg aagtagggat
    20581 ttgactcccc catcttcggc cgggagaagc gcttccgtgc cgcagggcgc aggactcccg
    20641 cctcgacgtc tagctcgtcc tgccgatctc tgaaaacccg gcgaccctcg tagggctcgc
    20701 acttcatccc cggcgcgtaa cgaataccga gggtctggaa catctcgctg acgcggcccg
    20761 gcatgccgag atcagccagt cggttgatcg tctccgccgc cgcgtcgtgc gcgcgaatcg
    20821 catcctcgct gagccggtag cgctcgaccg cccagcggta ctcgccgagc cgcgtcctcg
    20881 cggcggcaag catcgcctgc cggtcggcct cgctggcctc acggtcctca agcgcgcgaa
    20941 tcgcggcttg cactcgctcg agctcgtcgc gggccttgcg tagcctcccg gacgctcccg
    21001 aggggctcgg ctttccctcg agttccgcga caccgatctc ctgctcgaga tcggcgacag
    21061 cctgctcgca ctcagtgagc cgggtatgca gcttgtcggc gctcgggata gtggcggtgg
    21121 gggtcatgcg gtcctctctc tcggagcgcg ccgtatctcg gcggcgtggt ctgcggcggt
    21181 catggcggcg ggcgacttcc tgccgccctc ttgcagctcg ggcggcacct cgagcccggc
    21241 gtcctccaac gcctcgcgtg ccgcctcgcg gtaatcgtca ctcacgggtt cggtgccgtc
    21301 ctgctggtgg cgaaggtggt cttcgacggt ccagtccttg tggggcttgt ggcgtttcat
    21361 gcacttttcc tttgccgttt catcggggct ttcgtttcgc ggcagcacgg gctcgacgcc
    21421 gatcggcggc caagccggca gggtcgcgct ggcgcttggt tctgcggtta cggcaggtat
    21481 gggagcagag caggcgacgg ccgccatacg gacggtcgaa ctcgtcgccg cactcgatgc
    21541 acgcgacccg ctcgacttcc gctcggctcg cccgcgcctt cgcgcgcctt tcggccgccc
    21601 tttccctacc ggaacgctgg gctttctcga ggttttcggc tcgccaccgc cggctccgct
    21661 cgaccgccgc caagcgtcga cactcgtccg agcagaaccg cgaatccgcg cgagagggcg
    21721 aaaactcgtc gccgcactgt ccgcactcgc ggactccgtg cgcgctcact ggggcacccc
    21781 tggaatcgtg gaaacccgta caaacccaga gtggcagccc tcttcggtgc cgccggcagc
    21841 atatatgcgg ggtacccccc ctggggccgc cgaggctcgc tcgccctcgc gccgaacggg
    21901 ggctagcggc aggacaacaa accgagcggg gccgcgcggg ggcgtgtgac gcggtagcgg
    21961 tgcgccacga taacagtgag cgcaacggcg ccggttgccg aggatcaagg cttcgcagcc
    22021 gctcctctcg cagggcttga ggggatacag gctcatggtc gcaccgcccc gccggactcg
    22081 acgaaggcgc gcagttcggc cttgctgaac agcagccgtg agccgtcttt gtgaaacggg
    22141 attcggccgg ctgacttcag ggcgtagagc cgctgaggtt tgcaggcgag gtacttggcg
    22201 gctccgttga catcgagcca ctcgtcggtc gcgggtacag atgtacccac tcgctcggcg
    22261 agcttggctg ccagtcgatc gagcgcgtcg ggtcgctcgg ccaggcgatc gacgaggtca
    22321 tccagaaagg cgtccagggt ggctggactc atggctcggc cctccgctcc cgctggcacc
    22381 actggcagac gctgggctgg cgtccacctc taggtgacag cgccggaact cgacaatggc
    22441 agccggcgaa ctggtcacgc tcgggcgccc gcttcgcgtt gatgcctgcc gcctgcagct
    22501 cggctgcgaa gtggtcggcc atccggggcg cgatagtcgc gtcgcggcga tagctgccgt
    22561 cgatgcggag gaccacgtcc tcgctgtcgt gatcggcgga cggcaccacg tacacgtcaa
    22621 gccaatcggc gcgggccttg agtagatcgg caaggctcag ggcactcatg ccgacacctc
    22681 gcggatctcg cggcggcagg tggcgcagcg tgggggaccg gagccgcgag ggctgggtgc
    22741 gggcacgcgg cagccgcaga agccctccgc taagtcgttg gacctcttgg ccttcttggc
    22801 atcgttggca tccttggcct cctggggtag agggccaagg gtgccaagag ggccaagggt
    22861 gccatcatct ttacaagggg tctctacacc cttcaggacc caatgccacc ccgtgatccc
    22921 gctctcggag cggctttgct cgctgacaac gccagccggc ctcttggccc tcttgagtgt
    22981 ccgctcggag atgccgacct cccgagccgc cgcaaggatg tccttcgcat ccatcggtcc
    23041 gttctcgagg gtgaggcgta gccagtcgac cgcctcgtct ccggccgtgc gcgtctcgac
    23101 atcggacacg acctcgtcgg ccgagatcgt cttgtcgtca ccgcggacca gcgtgggcgc
    23161 cttgatcccc tgcgagaccg tgcgctcgtc gatcgtgaac gtcgcgctgt cgctcgaccg
    23221 ggcaaggttg cccttcgccc tggtgagcgc tttcgacgag ccttgatcgc cttccgggtc
    23281 ggaggggtcc ggcccccaga tcagcaccga ccgagcgagc tgcgggatgc cctggctgtc
    23341 ggcgatgcgg gcgagcgcat cgccggcatc ggcacggcgg tttagatgct ggacgagcag
    23401 gatcgacaga ccccgctcca tcgccaagtg gcggaacggc tcgaagaacg accgcacgtc
    23461 ctggcccttc cacgagttga ccccggccgg catgtacgcc tgcaccgggt cgacggtgac
    23521 cagggcgacg tctcccatct cgtcgcagag cgacgccagc cggtcggcgg tgaggatgat
    23581 gtccgtctcg atccagatgc gctcgagatc ggcgccggcg gcctcgagcc ggggccggat
    23641 cgtgtcggcc gcgtcatcct cggcgttcca catgatcgtg ttcgccgagc ggccgaggtt
    23701 gatcccctta ccgttcgtca cggccgcgtg gagccagcag gttgcgaggc tcttggcctg
    23761 gcccatctga ccggcgaggg cgcttagctt gccgagcggc aggtatggga accagagcca
    23821 gtcgacagct cgcgggacga ccatgctcgc ccgtccgccg ctcgcgcgtc gcggagcagg
    23881 gccgacggcg tcaagccctt tgagctcggc gaggacttga tcacgaagcg gcgcgaacgt
    23941 ccgcttgacg tcgtcgtggt cgcagcctgc caggcagtga atcgagagcc tgtcgccgtg
    24001 ccggtcgatc cgcgccgcgt ggccgctcga cagcggacag tcaatgccgt ggcgcgggtc
    24061 cgggccttcg tccagcgcgt agacgagccg ggtggcggac ggcgctgcag tgagcggcgt
    24121 cacggtggcg gcgctcacgc tgctctccga tcccgaagac gcgctcgcag ctcgcggtcg
    24181 agcgcttggg cttcgccaag tgccgcggtc agttcctcga ccatctccgc gatctcgtcg
    24241 gtcgatgtga cggtgagcgc gtcgtcgatg ttcacgacgg acagcacccg ctctcggggg
    24301 tcgtctggcc ccgtggctat gcgaccgcga ctgacgagct tccagagtcg ctccttggcc
    24361 ttctcggcgc gttggcgctg tcgtgcggtg agctcgtccg ttggaacccg tgacggcgtg
    24421 tcacaggttt cgtggtcgat cttgccgacg cacacgaccc cgttgccatc tggggagacg
    24481 aagacccggc ccgtctcatc gacgagttgg ctgccgggat gctggaggat gaggtcgtag
    24541 caacgctcaa gcacgggatc acggccgtta tcacggcggt agacgacctc cccgtcgtcg
    24601 taggtgatgg tcgaggtggg cggcgtcggc gccggctcct cgatctcgcg gaccatcgcc
    24661 cgcacatgct tggcctcggc ctcggggccg tgcttctcga cgaccttcgt ccaaacctcg
    24721 ctcaggcgct ccgggtcgtc gcgaagtggg gctagctcgc gagcagcgcg ctcgctgcgc
    24781 gggggctcca aagtggcacc cgtgggtgcc actttggcgt ggtcgtacag cgcgtggtgg
    24841 atctcggccg ccgcgatctg gcgatcagcg ttggtccggc tccatcccca gcgcttacgg
    24901 cagtagtcct cgaacgagga gaagtccttc ttgtagagcc tgttgtcacg gatcgtgcgg
    24961 agcgcatcgc cagcctcggc gtatgcatag acggcgcgct cgatcacgcg ctcgcagtcg
    25021 cacagcgcga gcgcaggcgt catctcgccg gcgctcatgc gacacctcga gctacccggt
    25081 agaggacggc tactagagca cggcctaccc gccggctacg tgactcgccg ggatgctgct
    25141 ggcggccccc atggggccgc ggtgagggtg gttcgtctgg ttcgccagaa ccgctgtcgt
    25201 cgccggacga cgacgtggcg cggccgggac gacggttggc cggtcgaccc gagccacgtc
    25261 gatttccgcc acgggagaca tgcgggcgcc gcgcgcagag aatcgcccca tcgaagctcg
    25321 aagcgttagc gggctcatac cgaccctcgc gagcagccga tgcaatcgtg tgaaggtgcc
    25381 cgcgcgtcac tccgcgctcg cgctctacag cgtcgtcctc tatcggtccg cgcgcggtcg
    25441 tgaccgtcag gatcatcggg cgccccgccg ggttggcaac ctcgcagcgc tccacgctgt
    25501 atccatcacc attcgagcgc cgcggaagct gccgcggcgc tgagggccgg cgctcgccaa
    25561 gccgacgagg gcgacggggc tgctgctgca tccggcgaac catgtcctcg acggacatct
    25621 cggtcggatc ggtgttcggc gcgtcgtcta gccgcgcatc gctgggctgg ggcatgttgg
    25681 tctcatggtc gagtacacgt cgtcgtaggg gtgaagttag ggatggcggt ggacggaagc
    25741 cgcccggaac gcatgagagg cgccccgatc ctgggacgcc tctctgacga cgcgagactc
    25801 gaccaagaag ccccgacgca tctgaagtta gggatggcgg tggacggaag ccgcccggat
    25861 gcagcgtgtt gccctaggcg ttgccctgag actttgcgaa ccgttaccgc tggtaacggt
    25921 tcttcgcgct gctactgcga tagctccggg tggactcgaa ccaccgacct cacgattatg
    25981 agtcgtgcgc tctaaccagc tgagctacgg agccgtggag ggtcgattct aggggcaggg
    26041 cggcgcgggg ccggagtggg ggaagatcgg ccgatgagcg aggtcgtcgc gcccgagctg
    26101 ctgatggcgg tgccgaacgt cagcgacgga cggcatgcgg agctgttgga ggcgatcgag
    26161 cgggcgtacg cgccggcgcg gttcctcgac ctgcacgccg accgagacca caaccggtcg
    26221 gtgctcaccc tggctggcag gcagggcgag ctggcggacg ggctggtgtc gggggcgcgc
    26281 gcggtggcgg agtggatcga cgtcagcgtg cacggggggg tccatccgta cgtcggagcg
    26341 ctcgacgtga tgccggtcgt ctaccgctcg caggcgcagc gcgggccggc gtgcgcggag
    26401 ctgctgaccg ccgcggcgcg gatcggggcg gagctggagc tgccggtgat cctctacggc
    26461 gagctcgcca cgcggcgtga gcaccgcgag cgggcctggc tgcgcgaggg cggccccgtc
    26521 gcgctcgccc ggcgcatcga gcacggcgag atcgtgccgg acttcgggcc agcccgtccc
    26581 catccgaccg cgggcgtggt cctcgcggcg gcgcgaccgc cgctggtcgc gttcaacgtc
    26641 gacctcgcga cgcctgatgt ggcggtcgca aggcggatcg ccgcgcgcat ccgcgagtcc
    26701 aacggcggcc tgcccggcat ccgcgccatc ggtctctggc tcgagcagcg gggctgcgcg
    26761 caggtctcga ccaacgtcca cgaccaccgt gcgacgccgc tgcgagagat cgtcgaggcg
    26821 gtccgtgctg aggcgccggt cgccgaggcc gagctggtcg ggctcgcgcc ggaggcggcg
    26881 ttcgagggct tcccggacga cgtgccgctg cggggcttct cgccggggcg ccatctgatc
    26941 gagaacgtgc tggccgcgat cggctgagcg cggccctggc gccacgccgg tcggtaggcg
    27001 ctgggatacg ctgctgggaa tggcccaagc gaagaccaag cgccgtcgca agcaccgtgg
    27061 gaccgctgcg gggacgatcg agcgcgcggg ccgcaccggc aagccgcaga cgcgcgagga
    27121 cgccaagcag atcgcccgcc agcggcgcgc cgagcggctc gacaagccgc cggagtggaa
    27181 gagcacgatc aatcgcgccg cgatcgccgc gatcgtcttc gcggtcctcg tcgtcgcgct
    27241 gttcggccgc ccgatcgttt ccggcctgct gctcggcgcc gtgatgttcg cgatctacgt
    27301 cccgctcggc tacgccaccg actcggcgat ctaccgcttc cggacgaagc ggagatcgtc
    27361 ggggggctcg cgccccccgc gccccccgaa ggcgtctggc cggtaggccg gccctcggca
    27421 ttccggccgg cgtttgacag gtaggctcag atcgtggaag tgcgcatgtt cagcgtcggg
    27481 ccgatccagg agaactgctt cctgtttcgc catgacggct cggagcgcgc gctgatcgtc
    27541 gatccgggcg acgaggccga gcggctgctg gcggcgatcg gcgagctcgg cgtgacgctc
    27601 gacggcatcc tgctgaccca cacccacttc gaccacgtcg gcgcggtcgc gccgctcgcg
    27661 aaggcgaccg gcgccgaggt ttgggtgccg gagatcgaga agccggtgct cgctgacatc
    27721 aacagctacg tcccgtggcc cgagttcggc ccgttcgaga gctgggacgc cgagcacacg
    27781 ctgaccgggg gcgagcggct ggagctcgcg ggcttcgaga tcgacgtgct gttcaccccc
    27841 ggccacagtc ctgggcacgt cagcttccac attcccgccg agcaggcgat cttctccggc
    27901 gacgtgctgt tccaggggtc ggtcgggcgc accgacctgc ccggcggcga tccgcaggtc
    27961 ctgatggaga cgttgaagtc gctggccgag acgctgcccg acgagacgag cgtccatccg
    28021 ggccacatgg ggatgacgac gatcggcgcc gagcgcgcga ccaacccgtt cctcggccag
    28081 ttcgcacgct gacgtggccg ccaagctgca ggccccgcgg ggcaccttcg acgtgctgcc
    28141 cgacgacggc cagcgccggc gcgagctcta ccgccgcgcc ggctcggtgc tggggcgcta
    28201 cggctaccgc ccgttcgaga cgccggtgtt cgaggacacc gacctgttcg cacgcggggt
    28261 gggggagtcg accgacatcg tgcgcaagga gatgttcacc ttcgaggacc gcggcgggcg
    28321 gtcgctgacg ctgcggcccg agggcaccgc cggcgtcgcg cgtgcctacg tcgagcacgg
    28381 catgcacaag ctgccgcagc cggtcaagct ctggtattgg ggcccgttct tccgccacga
    28441 gcgcgcccag gccggccgct accgccagtt cgcgcaggtc ggcgtcgagg cgctcggctc
    28501 cgacgacccg tcgctcgacg ccgaggcgat cctgctgctc gcggagctgc tcgaggcggc
    28561 cggcgtgcgc gaggtccggc tgcggctggc gagcctcggc tcgctcgacg cgcgcgcggc
    28621 ctatctcgac gagctgcggc gctatctgcg cgagcacgac ggcgagctct ccgacgaggt
    28681 ccgcgaccgg atcgatgcca acccgctgcg tgccttcgac gccgaccatc cgggcacacg
    28741 cgccgtcatg gagaacgccc cgcgcctgct tgacagcctc gccggtgacg acgccgagca
    28801 cttcaccgag gttcgcgccc tgctcgacgg cgccggcgtc tcctacgacg tcgatccgac
    28861 gctggtgcgc ggactcgact actacacgcg cacggtcttc gagttcgaga gcagccggct
    28921 gggagcgcag agcggtgtcg gcggcggcgg tcgctacgac ggcctcgtcg agcagctcgg
    28981 cggcccggcg acgccgggcg tcggctgggc ggccgggatc gagcggatcc tgctggcgac
    29041 cgacggcgag ctcggcggct tcccgcggcc ggtcttcgtc gctgtcgccg acggcggcga
    29101 ccggcgcgcg gcgttcgcgc tggccaccgc gctgcgcgag gcggggatcc gcagcgagct
    29161 cgagcaggcc ggccggtcgt tgaagggcca gctcaagcac gccgaccggc tcggcgcgaa
    29221 ggcggtcgcg atcgtcggcg aggggatcga gtggaaggac atgggaacgg gggagcagcg
    29281 agcggtgacg aatcagcagg aggcggtccg gatcgccgtg gggctcgacg catgaccgcc
    29341 gagagcaggg ggactggggc cggccacggg accgcggggg gcccgggggg
//
LOCUS       DATABV010000176        10473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2465181, whole genome shotgun sequence.
ACCESSION   DATABV010000176 DATABV010000000
VERSION     DATABV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465181"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VHF88_07750"
     CDS             <1..1206
                     /locus_tag="VHF88_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161699.1"
                     /translation="VPLPKDSTIIIRPRSALGLKYVEITKGKSSQGYAEGATIPLAAT
                     SQTETQEIDDFFNMFDEPTRVGSRTNLETFGAGFAGRGESLNRTFAELGPVVEKLEPV
                     MRNFNAPRTAWARFFPALEQAAAEVAPVAQEQADLWAALDTTFTAWASVSDSLQETIS
                     EGPPALETATRELPRQRPFLAHNEELFRRFRPAFAYLADAAPGLASAFRVGTPALQRS
                     PGFNRRLTRTLSTLERFGDDQRVPSAFARITNTVEVLRPTLAFLTPAQTTCNYGTLFF
                     RNIASMTSESDVVGSFLSVNPMALPVAPGSEGGPAAVPANGPPAPPAGPNLTISQRDL
                     LVDNYLHSNPYPNTAAPGQPAECEAGNEGYRAGINGENIQAIGNPANPGGLITEETVR
                     VLPKRGGGQ"
     gene            1203..2783
                     /locus_tag="VHF88_07755"
     CDS             1203..2783
                     /locus_tag="VHF88_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2161700.1"
                     /translation="MRAFRSGMSPVRAGLLALVVITIATFFAFSKDIPFTQTYRIQAV
                     FENSNLVAPRSPVRIAGIDVGKVVEVERYKDSDMSVVTMEITDDGRPVGEDATVKVRQ
                     RLFLEGNFFVDLKPGTPGAKELPDGGMIPVTQTSAPVQLDQILTTLQADTRRNLQDTL
                     IGFGEALDSEPSAADNADQDPDVRGLTGAQALNKALETAVPALKGSAIGAESLRGTEP
                     RDLSRMVKGIAGVSRGLAQNETQLSEFLTDFNTTMAATASRAPQLEETIRLLGPTASN
                     LRKGLASVDAALPPTQRFSRAILPGVRETPITIAASEPWLDQAIPFFSAQELGGLLKQ
                     ISPATRDLARATHESRPLVRESDDFNRCITEVIIPTGNIKVDDGEFSSNTENYKEFWH
                     AVAGQAGEGGGFDGNGPFLRLSAPGGAIPITSGRTNYQDQALHANASQPLLRTAPAFP
                     SSPPPLRRDVPCHRNPVPDVNGSFSVGPADGSRANAARPPTPPRGIGAQLVRDVEREA
                     AASQRLVPLESLLEGGAR"
     gene            2780..4156
                     /locus_tag="VHF88_07760"
     CDS             2780..4156
                     /locus_tag="VHF88_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2161701.1"
                     /translation="MRDIFAKALRSNLKAAVALLGLIVLGVAVGSYILSNQRLNPPAW
                     MPIVGEDTFEIKAEVESAQGILPGQGQAVNVSGVKVGDIASVHLEEGRAIATLRIEER
                     FARVYPNASILLRPKTGLKDMVVELDPGSPSSGDPLDEGAMLSNKQTLADVNFDEFLA
                     SLDGDTQDYLKLLVGDAGRTLRDGGGRDLANTFRRFQPLSRDFAKANKYVVQRRQKLK
                     RVIHNFSAIMTELGAHDRELARFVGGSAEVFRRFANQNENLSETIELLPGALRSGNTA
                     LAKIDRLGRSMESTFTNLEPTARALGPSLRQLRPFFAQTEPVIQRQLRPFTEEVRPIA
                     RDLRRPARNLSRSIPDLVKFTEVFNALFNELAYDPPGEGKGKNGYLYYLPWANHNTNS
                     IISSQDGISTLRRSLIMISCSQLNALEVFSKPSEITGEIINPYLATLIKLLNAPSTEE
                     RCGTGASQ"
     gene            4153..5649
                     /locus_tag="VHF88_07765"
     CDS             4153..5649
                     /locus_tag="VHF88_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2161702.1"
                     /translation="MIKQAPRPFQIFAMVAFVMACVGVLLYLWLSFGGVVPLRSEGYR
                     FQVRFPEATQLAEQADVRISGVPVGKVIKIDTGPNNTTEATLEMRSRYAPVASNARAM
                     LRQKSLLGETYVDITTGDDSRGSIPDGGLLPRSAVAPTVELDEIFRTFDDDTQQAFQT
                     WMQSQAASVNGRGTDINATFGNFPEFVEASDDILTELHAQSRAVSGTIRGTGEFFSAI
                     SERDGQLAHLITESNRLFQTTAARNRQFAAIWEELPDFSRQSRLTLPRLTEFANRADP
                     VVQQLQPAATEFAGTFKQLDRLSPEFEGFFRGLGPLITKSRRGVPALESLMRDFPPLL
                     ADFEPFLRNFNPLLAHLGQNRREISSFFGNVTATTTGRNPQLNPRISDVDPIHFLRAS
                     SSLSPQGLAYYPRALGQTRSNPYMAPGGLDRLASGLLSFETRTCANGDPAPPAQTGSP
                     ELVELAQLAEQYAFRTTGGDRNVARPPCVGQGAYPGFSTQFPQLEPEP"
     gene            5837..6502
                     /gene="pdxH"
                     /locus_tag="VHF88_07770"
     CDS             5837..6502
                     /gene="pdxH"
                     /locus_tag="VHF88_07770"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX2161703.1"
                     /translation="MPAADDAAARLESLRDDYELGALSEDALAPTWLEQFERWLAAAL
                     GAGMPEPNAMVLATAGADGTPGARTVLLRGIDARGFRFNTNYRSRKGRELDRNPRATL
                     VFPWYQQQRQVIVDGDVSPLRPDESDDYFAKRPRAARIAAAASPQSEVIESRERLERR
                     FAELEAELEGVEDPPRPEHWGGYLVAPRSVEFWQGRRNRMHDRLRFRRTAAGAWVVER
                     LAP"
     gene            6544..7200
                     /gene="msrA"
                     /locus_tag="VHF88_07775"
     CDS             6544..7200
                     /gene="msrA"
                     /locus_tag="VHF88_07775"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX2161704.1"
                     /translation="MFDFLSRREPELPTVEEALPGRDTPIVVPGEHFVLGTPIGPPFP
                     EGSERAIVGMGCFWGAEKVFWQLPGVYTTAVGYAGGITPNPTYEEVCSGRTGQNEAVL
                     VVFRPQEVSYEQILKAFWENHDPTQGMRQGNDVGTQYRSAIYTTSDAQREAATASAAS
                     YAEALAAAGHSAGITTEIEPAGVFYYAEDYHQQYLAKNPAGYCGLGGTGVACPVGLAT
                     "
     gene            complement(7218..7988)
                     /locus_tag="VHF88_07780"
     CDS             complement(7218..7988)
                     /locus_tag="VHF88_07780"
                     /inference="COORDINATES: protein motif:HMM:NF006100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX2161705.1"
                     /translation="MSDEQAVLTERRGRVLLITLNRPDARNAVDAALAQGVAAALTEL
                     DDDGDLQAGVLTGAGKGFCAGMDLKAFVSGESPHVEGRGFAGIAQRSADKPLIAAVEG
                     FAVAGGLEVALACDLIVAARGAKLGIPEVKRSLVAAGGALLRLPQRIPYHVAMELALT
                     GDPIPAERGHELGLVNRLADPGSAVDVALELAEAVARNAPLALVASKRIVVESASWDP
                     NEAWVKQGEIAGPVFVSEDAREGATAFAEKREPVWRGR"
     gene            complement(8076..8456)
                     /locus_tag="VHF88_07785"
     CDS             complement(8076..8456)
                     /locus_tag="VHF88_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2161706.1"
                     /translation="MSPSNLLPIAALLAALAFPASAVPLERDDEAPARAAATKTVRVV
                     DGAFSPKRLTVAKDTKIKWVWSKRNVLTHTVYLDDRPKGVRQFRSAPASRSATFTRKL
                     RKAGTYKLLCTFHSNMRMRIDVER"
     gene            8549..9226
                     /gene="nucS"
                     /locus_tag="VHF88_07790"
     CDS             8549..9226
                     /gene="nucS"
                     /locus_tag="VHF88_07790"
                     /inference="COORDINATES: protein motif:HMM:NF002876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEX2161707.1"
                     /translation="MRLIVARCEVRYTGRLTAVLPEALRLLMIKSDGSVMVHADTGGF
                     KPQNWMTPPTVIEEEPGRIVVRKHAGKTEDRLDIRVVEVVSDVTHDMGEAAALEKEGM
                     ERELQELLADAPQHCGEGFRLVRREWGTDIGPVDLMCRDGDDGWVAVEIKRVATIDAV
                     EQLTRYLERLAADPSFTSGCRGVLAAQQIKPQARVLAESRGIACVEVDIDVLRGEREP
                     DLTLFAA"
     gene            9269..10429
                     /locus_tag="VHF88_07795"
     CDS             9269..10429
                     /locus_tag="VHF88_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2161708.1"
                     /translation="MTERSGGHVAYRAVLLAAALIVFGLLFRQLLTLLLAVLITVIMA
                     IVISAVADRLERLDIPRPIGALLAVLAGLAVVAGLLWVLVPPFVDQTNEFAEDVPGIV
                     DDLRDQIHDLTGAEPGEISDQVDQFVDNYTDDPGKLIGPITSISLSVFGALAALILML
                     ITAYYMAIRPEPLLDGLRRLAPPRYRDKADRIMRRLRGAWVGWMQGVAADMLISGVLL
                     YIGLTLIGLDFAIFFAVLTALFSVIPYFGAFASGIPPVLFALTDSPGKALLVIVIYVA
                     VQQIEGNLTIPLVMSRTVKLHPAVIAGGVVIVGQLFGFVGLIVAVPILSMISILAEEL
                     WVQPLEENDRAGPLGPGQTSVQLRQGDGDRGDDADHDHDLHPDPEAGHGARS"
BASE COUNT         1610 a         3799 c         3529 g         1535 t
ORIGIN      
        1 gtcccgctgc cgaaggactc gacgatcatc atccgcccgc gctcggcgct cggactcaag
       61 tacgtcgaga tcaccaaggg caagtcgagc cagggctacg ccgagggcgc gacgatcccg
      121 ctcgccgcga cgtcgcagac cgagacccag gagatcgacg acttcttcaa catgttcgac
      181 gagccgaccc gcgtcggctc gcggaccaac ctcgagacct tcggcgccgg cttcgcgggg
      241 cgtggcgagt cgctcaaccg gacgttcgcc gagctcgggc cggtcgtcga gaagctcgag
      301 ccggtgatgc gcaacttcaa cgcgccgcgc accgcctggg cgcgcttctt cccggcgctc
      361 gagcaggccg ctgccgaggt ggcgccggtc gcgcaggagc aggccgacct ctgggctgcg
      421 ctcgacacga cgttcaccgc ctgggcctcg gtcagcgact cgctgcagga gacgatctcg
      481 gagggtccgc cggcgctcga gacggcgacg cgcgagctgc cgcgccagcg cccgttcctg
      541 gcccacaacg aggagctgtt ccgccgcttc cgccccgcgt tcgcctacct cgccgatgcg
      601 gcgcccgggc tggctagtgc gttccgcgtc ggcactcccg cgctgcagcg gtcgccgggc
      661 ttcaaccgcc gcctgacgcg gacgctctcg acgctcgagc ggttcggcga cgaccagcgc
      721 gtgccgagcg cgttcgcccg catcaccaac accgtggagg tcctgcgtcc gaccttggcg
      781 ttcctgaccc cggcgcagac gacgtgcaac tacggcacgc tcttcttccg caacatcgcc
      841 agcatgacct ccgagagcga cgtcgtcggc agcttcctgt cggtcaaccc gatggcgctg
      901 cccgtcgcgc ccggcagcga gggcggccca gccgccgtcc cggccaacgg accgcccgca
      961 ccgcccgccg ggcccaacct gacgatcagc cagcgcgacc tgctcgtcga caactacctc
     1021 cactcgaacc cgtacccgaa caccgcggcg cccggccagc cggctgagtg cgaggccggt
     1081 aacgagggct accgcgccgg gatcaacggc gagaacatcc aggcgatcgg caatccggcc
     1141 aacccgggcg gcctgatcac cgaggagacg gtgcgcgtgc ttcccaagcg agggggcggg
     1201 cagtgagagc gttccgcagc ggaatgtcgc cggtccgagc cggcctgctc gccctggtcg
     1261 tcatcacgat cgcgaccttc ttcgcgttca gcaaggacat cccgttcacc cagacgtacc
     1321 ggatccaggc ggtcttcgag aactcgaacc tcgtcgcacc acgttcgccg gtgcggatcg
     1381 ccggcatcga cgtcggcaag gtggtcgagg tcgagcgcta caaggacagc gacatgtcgg
     1441 tggtcacgat ggagatcacc gacgacgggc gaccggtcgg cgaggacgcc accgtgaagg
     1501 tgcgccagcg gctgttcctc gagggcaact tcttcgtcga cctgaagcct gggacgccgg
     1561 gcgccaagga gctgcccgac ggcgggatga tccccgtgac gcagacgtcg gctccggtcc
     1621 agctcgacca gatcctgacg acgctccaag ccgacacccg gcgcaacctc caggacaccc
     1681 tgatcggctt cggcgaggcg ctcgactccg agccttccgc cgcggacaac gccgatcagg
     1741 atcccgacgt gcgcggcctg accggcgcgc aggcgctcaa caaggcgctc gagaccgcgg
     1801 tgcccgcgct caagggctcg gcgatcggcg ccgagtcgct gcgcgggacc gagccgcgcg
     1861 acctgtcgcg gatggtcaag ggaatcgcgg gcgtcagccg cggcctcgcg cagaacgaga
     1921 cccagctgag cgagttcctg accgacttca acaccacgat ggccgcgacc gcctcgcgcg
     1981 ccccgcagct cgaggagacg atcaggctgc tcggcccgac agcgtcgaac ttgcgcaagg
     2041 ggctcgccag cgtcgacgcc gcgctgccgc ctacgcagcg gttctcgcgt gcgatcctgc
     2101 cgggcgtccg cgagacgccg atcacgatcg ccgcctccga gccgtggctc gaccaggcga
     2161 tcccgttctt cagcgctcag gagctcggtg gcctgctgaa gcagatctcg ccggcgacgc
     2221 gcgacctcgc ccgcgccacg cacgagtcgc ggccactggt gcgcgagtcc gacgacttca
     2281 accgctgcat cacggaggtc atcatcccga ccggcaacat caaggtcgac gacggcgagt
     2341 tctcgtcgaa caccgagaac tacaaggagt tctggcacgc cgtcgcgggc caggccggcg
     2401 agggtggcgg cttcgacggc aacggcccgt tcctgcgcct atcggcgccc ggcggcgcga
     2461 tcccgatcac gtcgggcagg accaactacc aggaccaggc gctgcacgcg aacgcgtcgc
     2521 agccgctgct gcgcaccgcg ccggccttcc ccagctcgcc gccgccgctg cgccgagacg
     2581 tgccctgcca tcgcaacccc gtgcctgacg tcaacggatc gttctcggtc ggcccggccg
     2641 acggttcgcg tgcgaacgcc gccaggccgc cgaccccgcc acgtggaatc ggagcgcagc
     2701 tcgtgcgcga cgtcgagcgc gaggcggcgg cgtcgcagcg actggtgccg ctcgagagcc
     2761 tgctcgaggg aggcgcccga tgagggacat cttcgccaag gcgctgcgca gcaacctcaa
     2821 ggcggccgtg gcgctgctcg gcctgatcgt gctcggcgtc gccgtcggca gctacatcct
     2881 gtccaaccag cggctcaacc cgccggcctg gatgccgatc gtcggcgagg acaccttcga
     2941 gatcaaggct gaggtcgagt cggcccaggg catcctgccc ggccagggcc aggccgtgaa
     3001 cgtctcgggc gtcaaggtcg gcgacatcgc cagcgtccac ctcgaggagg gacgggcgat
     3061 cgcgacgctg cggatcgagg agcgcttcgc acgcgtctat ccgaacgcgt cgatcctgct
     3121 gcgcccgaag acgggactca aggacatggt cgtcgagctc gatccgggca gcccctcgtc
     3181 cggggatccg ctcgacgagg gggcgatgtt gtcgaacaag cagacgctcg ccgacgtcaa
     3241 cttcgacgag ttcctcgcca gcctcgacgg cgacacccag gactacctca agctgctggt
     3301 cggcgacgcc gggcggacgc tgcgcgacgg cggcggtcgc gacctcgcga acaccttccg
     3361 ccgcttccag ccgctctcgc gcgacttcgc caaggcgaac aagtacgtgg ttcagcgccg
     3421 ccagaagctc aagcgcgtga tccacaactt ctcggcgatc atgaccgagc tcggcgccca
     3481 cgaccgcgag ctcgcccgct tcgtcggcgg cagcgcggag gtcttccgcc gcttcgcgaa
     3541 ccagaacgag aacctctccg agacgatcga gctgctgccg ggcgcactgc gctcgggcaa
     3601 caccgcgctc gccaagatcg accggctcgg ccgctcgatg gagagcacgt tcacgaacct
     3661 cgagccgacc gcacgggcgc tcgggccgag cctgcgtcag ctgcgaccgt tcttcgccca
     3721 gaccgagccg gtcatccaac gccagctgcg ccccttcacc gaggaggtcc ggccgatcgc
     3781 gcgcgacctg cggcgcccgg cgcgtaacct ctcgcggtcg atccccgatc tggtcaagtt
     3841 cactgaggtc ttcaacgcgc tcttcaacga gctcgcctac gacccgcccg gcgagggcaa
     3901 gggcaagaac ggctacctct actacctgcc gtgggcgaac cacaacacga actcgatcat
     3961 ctcgtcgcag gacggcatca gcacgctgcg gcgcagcttg atcatgatct cgtgcagcca
     4021 gctcaacgcg ctcgaggtct tctcgaagcc gagcgagatc acgggagaga tcatcaaccc
     4081 gtacctcgcg acgctgatca agctgctcaa cgcgcctagc actgaggagc gttgcggaac
     4141 gggggcgtcg cagtgatcaa gcaggctcca cgtccgttcc agatcttcgc gatggtcgcg
     4201 ttcgtcatgg cctgcgtcgg cgtgctgctc tacctgtggc tctccttcgg cggcgtcgtc
     4261 ccgctgcgtt ctgagggcta ccgcttccag gttcgcttcc ccgaggcgac ccagctcgcc
     4321 gagcaggcgg acgtgcggat ctctggcgtg cccgtcggca aggtgatcaa gatcgacacc
     4381 ggcccgaaca acacgaccga ggcgacgctc gagatgcgct cgcgctacgc gcccgtcgcc
     4441 tcgaacgccc gcgcgatgct gcgccagaag tcgctgctcg gcgagaccta tgtcgacatc
     4501 accaccggcg acgactccag gggctcgatc cccgacggag ggctgctgcc gcggtcggcc
     4561 gtcgcgccga ccgtcgagct cgacgagatc ttccgcacct tcgacgacga cacccagcag
     4621 gcgttccaga cttggatgca gtcgcaggcc gcctcggtca acggtcgcgg caccgacatc
     4681 aacgcgacat ttggcaactt cccggagttt gtcgaggcga gcgacgacat ccttaccgag
     4741 ctgcacgcgc agtcgcgcgc ggtcagcggc acgatccgcg gcaccggcga gttcttctcg
     4801 gcgatcagcg agcgcgacgg ccagctcgcc cacttgatca ccgagtccaa ccgtctcttc
     4861 cagaccaccg ccgcgcgcaa ccgccagttc gcggcgatct gggaggagct gcccgacttc
     4921 tcacgccaga gcaggttgac gctgccgcgc ctgaccgagt tcgccaaccg cgccgatccg
     4981 gtcgtccagc agctccaacc ggccgcgacc gagttcgccg gaaccttcaa gcagctcgac
     5041 cgcctgagcc cggagttcga gggcttcttc cgcggccttg gcccgctgat cacgaagtcg
     5101 cgccgcggtg tgccggcact cgagagtctg atgcgcgact tcccaccgct gctcgccgac
     5161 ttcgagccct tcctgcgcaa cttcaacccg ctgcttgccc acctcggcca gaaccgccgc
     5221 gagatcagct cgttcttcgg caacgtcacg gcgacgacga ccggccgcaa cccgcagctc
     5281 aacccgagga tttccgacgt cgacccgatc cacttcctgc gggcatcgtc gagcctcagc
     5341 ccacagggcc tcgcctacta tccgcgtgcg ctcggccaga cgcgctcgaa cccctacatg
     5401 gcgcctggtg gcctcgaccg gctcgccagc ggtctgctct cgttcgagac gcggacgtgc
     5461 gccaacggcg atccggcccc gcccgcccag acaggctcgc ccgagctcgt cgagctcgct
     5521 cagctcgccg agcagtacgc cttccgcacc accggcggcg accgcaacgt cgcccgtccg
     5581 ccctgcgtcg ggcagggcgc ctacccaggc ttcagcacgc agttcccgca gctcgagccc
     5641 gagccctagg ccgccgagcc ggcgctagat ggcccattga ctccaggaat gggcacgttt
     5701 acgatcggcg caggcttcag ggggacacag gacgggcaga gatgcgcagg ctgggaccca
     5761 gcgggagcgg gttccagcca cgcgctagcg tcgaaggcgt gcagcccgat caggcgaagc
     5821 tcaggagcgg ctgaccatgc ccgcggcaga tgacgcggcg gcgcgactgg agagcctgcg
     5881 cgacgactac gagctcggcg cgctgtccga ggacgcgctc gcgccgacct ggctcgagca
     5941 gttcgagcgc tggctggccg ccgcgctcgg tgccgggatg ccggagccga acgcgatggt
     6001 cctggcgacc gccggcgccg acgggacgcc cggcgcgcgg accgtcctgt tgcgcggcat
     6061 cgacgcgcgc gggtttcgct tcaacaccaa ctatcgctcg cgcaaggggc gcgagctgga
     6121 tcggaacccg cgcgcgacgc tcgtctttcc ctggtaccag cagcagcgcc aggtgatcgt
     6181 cgacggcgac gtctctccgc tccggcccga cgagtccgac gactacttcg ccaagcggcc
     6241 gcgtgccgcg cggatcgccg ccgccgccag tccgcagtcc gaggtgatcg agtcgcgcga
     6301 gcggctcgag cggcgcttcg ccgagctgga ggccgagctc gagggggtgg aggacccgcc
     6361 ccgtcccgag cactggggcg gctacctggt cgcgccgcgc agcgtcgagt tctggcaggg
     6421 ccgccgcaac cgcatgcacg accgtctgcg cttccgccgc accgccgccg gggcctgggt
     6481 cgtcgagcgg cttgcgccgt gagccgtcgc tagcctggag ggcgttccta cagaggagac
     6541 gccgtgttcg acttcttgtc ccgccgcgag cccgagctgc cgaccgtcga ggaggcgctg
     6601 ccgggccgcg acacgccgat cgtcgtcccg ggggagcact tcgtgctggg gacgccgatc
     6661 gggccgccgt tcccggaggg aagcgagcgg gcgatcgtcg ggatgggctg cttctggggc
     6721 gcggagaagg tgttctggca gcttcccggc gtctacacga cggcagtcgg ctacgccggc
     6781 ggcatcacgc ccaacccgac ctacgaggag gtctgcagcg gtcgcaccgg ccagaacgag
     6841 gcggtgctgg tcgtcttccg gccgcaggag gtctcctacg agcagatact caaggcgttt
     6901 tgggagaacc acgacccgac ccaggggatg cgccagggca acgatgtcgg gacccagtac
     6961 cgctcggcga tctacacaac ctccgacgcc cagcgcgagg cggcgaccgc gtctgccgcc
     7021 agctacgcgg aggcgcttgc cgccgccggt cacagcgccg gcatcacgac cgagatcgag
     7081 cccgccggcg tcttctacta cgccgaggac taccaccagc agtacctcgc caagaacccc
     7141 gccggctact gtgggctcgg cggcacgggc gtcgcctgcc cagtcggcct ggccacctga
     7201 gcgctagcgg aggaggctca ccggccgcgc cagaccggct cgcgcttctc ggcgaacgcc
     7261 gtcgcaccct cgcgggcgtc ctcggagacg aacaccggcc ccgcgatctc gccctgcttg
     7321 acccacgcct cgttcgggtc ccaggacgcc gactcgacga cgatgcgctt cgacgcgacg
     7381 agcgcgagcg gcgcgttgcg ggctaccgcc tcggcgagct cgagcgcgac gtcgactgcc
     7441 gagccggggt cggcgaggcg gttgaccagc ccgagctcgt gtccgcgctc ggcggggatc
     7501 gggtcgccgg tcagggcgag ctccatcgcg acgtggtacg ggatccgctg cggcagccgc
     7561 agcagcgccc cgccggccgc gacgaggctc cgcttgacct ctgggatgcc gagcttcgcg
     7621 ccgcgggccg cgacgatcag gtcgcaggcg agcgccacct cgagtccgcc agcgaccgcg
     7681 aagccctcga cggccgcgat cagcggcttg tccgccgagc gctgggcgat gccggcgaag
     7741 ccgcggccct cgacgtgggg gctctcgccg ctgacgaacg ccttgaggtc catgccggcg
     7801 cagaagccct tgcccgcgcc ggtcagcacc ccggcctgga gatcaccgtc gtcgtcgagc
     7861 tcagtcagcg ctgccgcgac gccctgcgcc agcgccgcat cgaccgcgtt gcgcgcgtcg
     7921 gggcggttga gggtgatcag caacacgcgc ccgcggcgct cggtcagaac tgcctgctcg
     7981 tcgctcatga cggttgaacc taccggccac ctccagcctg cgcagggcca gctcggtgaa
     8041 gaaggtggtc tgggcgggca tcggcggccg gtcggtcagc gctcgacgtc gatgcgcatt
     8101 cgcatgttcg aatggaatgt gcagagcagc ttgtaggtgc cggccttgcg cagcttgcgc
     8161 gtgaaggtgg ccgacctgct cgcgggcgcc gaccggaact gccgcacgcc cttcggccgg
     8221 tcgtcgaggt agaccgtgtg ggtcagcacg ttgcgcttgg accagaccca cttgatcttc
     8281 gtgtccttgg cgaccgtcag ccgcttcggg gagaaggcgc cgtccacgac ccggaccgtc
     8341 ttggtcgccg ccgccctcgc cggcgcctcg tcgtcgcgct ccagcggcac cgcgctggcg
     8401 gggaaggcga gcgccgcgag cagcgcggcg atcggcagca ggttggacgg cgacatcggg
     8461 ctcctcctcg tctgggactc atggcatagc tttccagagc ccgcgagtca agcccagacg
     8521 tccggcaggc gtcgtagctt cagcggccgt gcgcctgatc gtcgcccgct gcgaggtccg
     8581 ctacaccggc cggctgaccg ccgtcctgcc ggaggccctg cgcctgctga tgatcaagtc
     8641 ggacggctcg gtgatggtgc atgccgacac gggcggcttc aagccgcaga actggatgac
     8701 gccgccgacg gtgatcgagg aggagccggg ccggatcgtc gtgcgcaagc acgcgggcaa
     8761 gaccgaggac cgactcgaca tccgggtcgt cgaggtggtc tccgacgtca cgcacgacat
     8821 gggggaggcg gcggcgctcg agaaggaggg catggagcgc gagctgcagg agctgctcgc
     8881 cgatgcgccg cagcactgcg gcgagggctt ccggctggtg cgccgcgagt gggggaccga
     8941 catcggtccg gtcgacctga tgtgtcgcga cggcgacgac ggctgggtgg ctgtcgagat
     9001 caagcgcgtc gcgacgatcg acgccgtcga gcagctgacc cgctatctcg agcggctcgc
     9061 cgccgacccg tcgttcacgt cgggctgtcg cggcgtacta gcggcgcagc agataaagcc
     9121 gcaggcgcgc gtgctggccg agtcgcgcgg gatcgcgtgc gtcgaggtcg acatcgacgt
     9181 gctgcgcggg gagcgtgagc cggatctcac gctcttcgcg gcttgagcga ccgcttcggc
     9241 atgcccgatc cggggtagcc atcgagcgat gactgagcgc agcggcggtc acgtcgcata
     9301 ccgggcggtc ctgctcgccg ccgcgctgat cgtcttcggg ttgctgttcc ggcagctgtt
     9361 gacgctgctg ctggccgtcc tgatcacggt gatcatggcg atcgtcatct ccgcggtcgc
     9421 cgaccggctc gagcggctgg acatcccgcg cccgatcggc gcgctgctcg ccgtgctcgc
     9481 tggactggcc gtggtcgccg gcctgctgtg ggtgctcgtc ccgccgttcg tcgaccagac
     9541 gaacgagttc gccgaggacg tgccggggat cgtcgacgac ctacgtgacc agatccacga
     9601 cctgaccggc gcggagcccg gcgagatctc cgaccaggtc gaccagttcg tcgacaacta
     9661 caccgacgac cccggcaagc tgatcggccc tatcacctcg atcagcctca gcgtgttcgg
     9721 cgcactagcg gcgctgatcc tgatgctgat caccgcctac tacatggcga tccgtcccga
     9781 gccgctgctc gacggcctgc gtcgacttgc gccgccgcgc taccgcgaca aggccgaccg
     9841 catcatgcgc cggctgcgcg gggcctgggt cggctggatg cagggcgtcg ccgccgacat
     9901 gctgatctca ggcgtcctgc tctacatcgg tctgacgctg atcgggctcg acttcgcgat
     9961 cttcttcgcg gtcctgaccg cgctgttctc ggtgatcccc tacttcggcg cgttcgccag
    10021 cgggatcccg ccggtgctgt tcgcgctcac cgactcgccc ggaaaggcgc tgctggtgat
    10081 cgtcatctac gtcgccgtcc agcagatcga gggcaacctg acgatcccgc tggtgatgtc
    10141 acggaccgtc aagctgcacc cggcggtgat cgccggtggc gtcgtgatcg tcggtcagct
    10201 gttcgggttc gtcgggctga tcgtcgccgt gccgatcctg tcgatgatca gcatcctcgc
    10261 cgaggagctc tgggtccagc cgctcgagga gaacgaccgt gcgggaccgc tcgggccggg
    10321 ccagacgtca gtacagcttc gtcagggcga tggcgatcga ggcgacgacg cagatcacga
    10381 tcacgacctg catccagatc cagaagcggg gcatggcgcg cgatcctagt gagatttatg
    10441 atcgccgcgt gatcggggcg atcgtcgtcg gga
//
LOCUS       DATABV010000177         4614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_940206, whole genome shotgun sequence.
ACCESSION   DATABV010000177 DATABV010000000
VERSION     DATABV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940206"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHF88_07800"
     CDS             <1..198
                     /locus_tag="VHF88_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161709.1"
                     /translation="LPVGDVIVSGEGSRDERLIADVAAQLGLPVAPAAPLGSLDASTV
                     DGDHDPHRYTVAAGLALGAAA"
     gene            195..869
                     /locus_tag="VHF88_07805"
     CDS             195..869
                     /locus_tag="VHF88_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX2161710.1"
                     /translation="MRPVNLLPESQRRRAPAGDGKSAYAVLGVLAVLLAMTAAYVTTA
                     NQATERAAAAAEAAAEAERLEAQIASLGAFGNFAQIKEMRIASVRQLAAQRFDWERLM
                     LELARVLPDDGWLKTASASTSGESDDTGTASASVGGVPTATLEGCLRYQSDVADLMLR
                     LRRLHRAEDVSLTESAREDAQEAPSVDGCGRYYRFEIAVTFAPTVVDESPDGQPRVPA
                     SLGGGS"
     gene            866..1795
                     /locus_tag="VHF88_07810"
     CDS             866..1795
                     /locus_tag="VHF88_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161711.1"
                     /translation="MSLTDRDRKIMLVLVPVLILAPFWFLLLSPKREQAATAGQELAA
                     QEQRRDDARIRAASLTTARTSFAADYAELVRIGKAVPTAVDMPTVLVQLEQAARGTDI
                     EFTRITAAEREAAVVPAATDDADPVDPGGEPAQSGPGAAGEAAGETAAAADQSAAAAE
                     QPGLDETDTQTSQPANDGALPVGGGTAPTSSASATGTAVPGLDTVGLELEFTGNFLDL
                     SEFFHRIKRYVELDGEKLAVRGRLLTVDGVEFSSEPDIFPKIRATVTATAYLAPAVEG
                     ATAGATPGGPGPVSASTGASAPPLAASTPMTGP"
     gene            1797..2780
                     /locus_tag="VHF88_07815"
     CDS             1797..2780
                     /locus_tag="VHF88_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161712.1"
                     /translation="MNELIQDTWHDLRAKRLWPVALALALAIVAIPVLLTKSAAQPPA
                     NAPVVTETDGDAEQQIALRLDGEAAGSTGTGSALDKFAEGDPFTPPAAVVERDAGSAT
                     ATASVGGPTADRPTDGDGATGGGGRTDSGGGGGPNVGSPPAAAPSPPADVDPPRTRTE
                     TTEYEYVADVTFWQGERRRTMRGLRKLDMLPSQRAPVLIFMGTAGDGGNAVLLVDSTL
                     KAAGEGRCVPEPSNCVYVHLGPGSEHAFTTAEGESYRLRVDEIRRVEVRPSTSRKRIR
                     PSARTSTGDGAAVRRFELPSLVDLVAVSETETVHAPAPAAEMRSRVSAGGR"
     gene            2789..4123
                     /locus_tag="VHF88_07820"
     CDS             2789..4123
                     /locus_tag="VHF88_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161713.1"
                     /translation="MRFSKAALLAAIAAAVALAPATPSTADAKPSKRSKAAKPKITRV
                     TPMRIEVGETLVIRGTRFKAKKRRNTIIFRGADGRTAFAKPRRASRRRLVVKVPGSVA
                     RLLAVHDGLQRPTRLKLRVLAGRFSAFTPRRLSPVVLGVGSGPGGEGGSGGRGAPTDG
                     THPLATCDSDADHDDDLLSNADELRDKTDPCLADTDGDGMTDGWEYLAAKDLNIKAVP
                     YPGNRPYPNPLDPSDRDTDFDGDFLPAGIEYRLWRYTGSSFDAGLVGGHVAGSPLGYS
                     DGTQASRPAEEPSPPAFRSPSYGIPFAPPAYPARHVMDHPGPFHAANWTDDERDADSD
                     GVNNYVETAGPGHFSWWVSYLEEASIEPWPDVYFGSFTQRPFVDLAPDDPDVDGDTLL
                     DGEDDQDNDDVLNFDEMYEPGAVYGPDSRQKNAFNPCAPDGESRTCPIYRPF"
     gene            4233..>4614
                     /locus_tag="VHF88_07825"
     CDS             4233..>4614
                     /locus_tag="VHF88_07825"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX2161714.1"
                     /translation="MSIRFTTAGESHGPGLTAVVEGLPAGVEVRPEDIDTDLARRQLG
                     HGRGGRMKIEKDRAEVTSGIRHGRTLGSPVSLRIANRDYANWEERMNPWPVEGEVAEV
                     HLPRPGHADLPGVQKYGFSDVRNVL"
BASE COUNT          676 a         1726 c         1610 g          602 t
ORIGIN      
        1 ctgcccgtcg gcgacgtgat cgtgtccggc gagggctccc gcgacgagcg gctgatcgcc
       61 gacgtcgccg cgcagctcgg gctccccgtc gcgcccgcgg cgccgctcgg ctcgctcgac
      121 gcctcgactg tcgacggcga ccacgacccg caccgctaca ccgtcgccgc cggcctggcg
      181 ctcggagctg ccgcatgagg cccgtaaacc tgctgccgga gtcccagcgc cgtcgcgcgc
      241 cggcgggcga cggcaagagc gcctacgccg tcctcggcgt cctggccgtg ctgcttgcga
      301 tgacggcggc ctacgtgacg accgccaatc aggcgaccga gcgcgccgcc gcagccgccg
      361 aggccgccgc cgaggcggag cggctcgagg cccagatcgc gagcctcggg gcgttcggca
      421 acttcgccca gatcaaggag atgcgcatcg cgtccgtccg tcagctcgcc gcccagcgct
      481 tcgactggga gcgcctgatg ctcgagctcg ctcgggtact gccggacgat ggctggctga
      541 agacggcgtc ggcgtcgacg agcggcgaga gcgacgacac cggcaccgcg agcgcatcgg
      601 tcggaggcgt tccgacggcc acgctcgagg gctgcctgcg gtaccaatcc gacgtcgccg
      661 atctgatgct gcgtctgcgt cgcctgcacc gcgccgagga cgtctcgctg acggagtcgg
      721 cgcgcgagga cgctcaggag gccccgtcgg tcgacggctg cggtcgctac taccgcttcg
      781 agatcgcggt gaccttcgcg ccgaccgtcg tcgacgagtc tcccgacggc cagccgcgcg
      841 tgccggcatc gctcggaggt ggctcatgag cctcaccgac cgcgaccgca agatcatgct
      901 ggtgctcgtt cccgtcctga tcctggcgcc tttctggttc cttctgctgt cgccgaagcg
      961 cgagcaggcc gcgaccgccg gacaggagct cgcggcccag gagcagcgcc gcgacgacgc
     1021 ccgaattcgc gccgccagcc tgaccacggc gcggaccagc ttcgccgccg actatgccga
     1081 gctggtgcgg atcggcaagg cggtgccgac cgccgtcgac atgccgaccg tcctcgtcca
     1141 gctcgagcag gcggcgcgcg gcaccgacat cgagttcacc cgcatcaccg ccgcggagcg
     1201 cgaggcggcg gtagtgcccg ccgcgacgga cgacgcagac cccgtcgatc cgggcggcga
     1261 gccggcccag agcggccccg gcgcggccgg agaggccgcg ggcgagaccg ccgccgcggc
     1321 cgaccagtcg gcggcggcag ccgagcagcc ggggctcgac gagaccgaca cgcagacatc
     1381 gcagccagcc aacgacggcg cgcttccggt cggcggcggg accgcgccaa cctcgtccgc
     1441 gagtgcgacg gggacggccg tgcccggcct cgacaccgtc ggcctggagc tcgagttcac
     1501 cggcaacttc ctcgacctct ccgagttctt tcaccgcatc aagcgctacg tcgagctcga
     1561 cggcgagaag ctcgccgtcc gtggccgcct gctcaccgtc gatggggtcg agttcagcag
     1621 cgagcccgac atcttcccga agatccgagc gacggtgacc gcgacggcat acctggctcc
     1681 ggcggtcgag ggcgcgacgg ctggcgccac gcccggtggg cccggccccg tctccgcgtc
     1741 gaccggggcc tccgccccgc cgctggccgc gtcgaccccg atgactggtc cctgaaatga
     1801 acgagctgat ccaagacacc tggcacgacc tacgcgcgaa gcggctctgg ccggtcgcgc
     1861 tcgcgctggc gctcgcgatc gtcgcgatac cggtgctgtt gacgaagtcg gccgcccagc
     1921 cgcccgccaa cgcgccggtc gtcaccgaga ccgacggcga cgccgagcag cagatcgcgc
     1981 tgcgactcga cggcgaggcc gccggctcga ccggcaccgg ctccgcgctc gacaagttcg
     2041 cggagggcga tccgttcacg ccgccggccg ccgtggtcga gcgcgacgcg ggcagcgcga
     2101 ccgccacggc cagcgtcggc ggaccgacgg cggaccgtcc gacggacggc gatggcgcca
     2161 cgggcggcgg tggtcgcacg gacagcggtg gtggtggcgg ccccaacgtc ggatcgccgc
     2221 cagccgcggc gccgagtccg cccgccgacg tcgacccgcc gcgcacgcga accgagacga
     2281 cggagtacga gtacgtcgcc gacgtcacgt tctggcaggg ggagcgccgc cgcacgatgc
     2341 gcggcctgcg caagctcgac atgctgccga gccagcgggc ccccgtgttg atcttcatgg
     2401 gaaccgcggg cgacggtggc aacgccgtcc tcctagtcga ctcgacgttg aaggcggctg
     2461 gcgaggggcg ctgcgtccca gagccgtcca actgcgtcta cgtccacctc ggacccggct
     2521 ccgagcatgc cttcacgacc gcggagggcg agtcctaccg cctgcgcgtc gacgagatcc
     2581 ggcgcgtcga ggtgaggccg tcgacgtcgc gcaagcggat tcggccctcg gcgcgcacgt
     2641 cgactggcga cggcgcagcg gtacggcgct tcgagctgcc gtcgctcgtc gacctcgtcg
     2701 cggtcagcga gaccgagacc gtccatgcgc cagcgccggc ggcggagatg cgctcaaggg
     2761 tttcggcggg cggtcgatag ggatgtcgat gcgcttctcc aaggccgctc tcctcgccgc
     2821 catcgcggcg gcggtggccc ttgcgccagc cacgccctca accgcggacg cgaagccgag
     2881 caagcgctcg aaggctgcca agccgaagat cacgcgcgtc acgccgatgc ggatcgaggt
     2941 cggcgagacg ctggtcatcc gcggcacgcg cttcaaggcc aagaagcgcc gcaacacgat
     3001 catcttcaga ggagccgacg gccgcaccgc cttcgccaag ccgcgccgcg ccagccgccg
     3061 caggctcgtg gtcaaggtgc caggcagcgt cgcccggctg ctcgcggtcc acgacggcct
     3121 ccagcgcccg actcggctca agctgcgcgt cctggccggc aggttcagcg cgttcacgcc
     3181 acgccgcctc tcgccggtgg tgctcggggt cggcagtggg cccggtggcg agggcggctc
     3241 aggcggccga ggcgctccga cggacgggac gcatcccctc gcaacttgtg acagcgacgc
     3301 cgatcacgac gacgatctgc tctccaacgc cgacgagctt cgcgacaaga ccgacccgtg
     3361 cctggccgat accgatggcg acgggatgac tgacggctgg gagtacctcg cggcgaagga
     3421 cctcaacatc aaggccgtcc cgtacccggg gaaccgtccc taccccaatc cactcgatcc
     3481 gtccgatcgg gacaccgact tcgacggcga cttcctgcca gccggcatcg agtaccggct
     3541 ctggcgctac acgggcagca gcttcgatgc cggtctggtc ggcggccatg tcgccgggtc
     3601 accgctcggc tacagcgacg ggacccaggc gagccgtccg gccgaggagc cgtcgccacc
     3661 cgccttccgc tccccctcgt atgggattcc gtttgcgccg ccggcctatc cggcccgcca
     3721 cgtgatggac catcccggtc ccttccacgc ggcgaactgg accgacgacg agcgcgatgc
     3781 cgactcggat ggggtgaaca actacgtcga gaccgccggg cccgggcact tctcctggtg
     3841 ggtctcctac ctcgaggagg cctcgatcga gccgtggccg gacgtctact tcggaagctt
     3901 cacgcagcgt ccgttcgtcg acctcgctcc cgacgacccc gacgtcgatg gcgacacgct
     3961 gctcgacggc gaggacgacc aggacaacga cgacgtgctc aacttcgatg agatgtacga
     4021 gcccggtgcg gtctacgggc ccgactcgcg ccagaagaac gcgttcaacc cgtgcgcgcc
     4081 agacggcgag tcgcggacct gcccgatcta ccggcccttc tgagtaggac cgtgggctcg
     4141 cgcccccgga cccccgcggc gcctggccgg ttgggctagg gctcgcgccc ccggaccttc
     4201 agggcgcgtg gccggttagg gtgtctggcc gcatgtcgat ccgcttcacc accgccggcg
     4261 agtcccatgg gccggggctg acagccgtcg tggaggggct tccggcgggc gtcgaggtcc
     4321 gtcctgagga catcgacacc gacctcgcgc ggcgtcagct cggacacggc cgcggcggtc
     4381 ggatgaagat cgagaaggat cgcgccgagg tgacgtcggg catccgccac ggccggacgc
     4441 tcggcagccc ggtctcgctg cggatcgcca accgcgacta cgcgaactgg gaagagcgga
     4501 tgaacccgtg gccggtcgag ggcgaggtcg ccgaggtcca cctcccgcgg cccggtcacg
     4561 ccgaccttcc cggcgtccag aagtacggct tcagcgacgt ccgcaacgtg ctcg
//
LOCUS       DATABV010000178         8888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1904045, whole genome shotgun sequence.
ACCESSION   DATABV010000178 DATABV010000000
VERSION     DATABV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904045"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VHF88_07830"
     CDS             <1..786
                     /locus_tag="VHF88_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161715.1"
                     /translation="AYAADVEDGGGSIALGCAVERIDSSAHEAVVRHARGTTVARHAI
                     ACAGAWAGHLAPSDQRTRFVHFRGAYKQLTAQRAGLVNGLVYPVPDPALPFLGMHFTR
                     TVDDRMILGPTALAVGAPDAYRIARIAPRDAGRALAWPGTWRMARRWWRTALREVSTA
                     VSNRAFLADARRYVPALERGDLVPAGAGVRAQPVARDGSLVDDFLLSVQGRVLHVASA
                     PSPAATSSLPLGRLIVERAHDALGLGDHRSRTQPPPPKEAERT"
     gene            783..2813
                     /locus_tag="VHF88_07835"
     CDS             783..2813
                     /locus_tag="VHF88_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX2161716.1"
                     /translation="MSSLRVAVDVGGTFTDICILDETGGSALRVAKVPTTADPIEGVL
                     EGVRHAGVPFGDVVLFSHGTTVATNALITRRLPPAAMVTTKGFRDVIEIRRGNRDDLW
                     DAYKDIAPPHIRRRDRLEVSERIDYAGEVVEPLDEDEARHVARVLRKREVAAVAVCLI
                     NAHANPEHEQRLAEILAEELPGVWISTSSDVLPELFEHERFSTTVSNAVLSPLVSNYV
                     SRLDAGLREGGYKGDLVLLHSGGGVMTAQTSERYAARLAASGLAAGAIASRHIATMCG
                     FDNAIGIDMGGTSTDISLVYDGEARVTKEWFVEFGYPILFPSIEVLTIGAGGGSLAWI
                     DEAGSLHSGPQSAGAIPGPAAYGRGGEEPTTTDANLVLGRLGGELIGGEMTLDRKLAS
                     EALRTKVAEPLGLDVDDAARAVTRVANANMADALRLISIRRGYDPREFCLVVFGGAGP
                     LHGVELARELSIPTVLVPLDPGITSAFGCLLVDVRHDLTQMYLTDAGSADPEDVEASF
                     QALEEQGREQLDTEGVPREQQELVRTIDMRYRGQWRSLSVAVGRPLRSLDDAIATFHA
                     EHEREYNFRRDDTPVEIYRLGIGAIGITPKPDLPRHELSDAPVEPVERRMVTFDGVEE
                     PREAAVYLRSDLHAGAHVEGPAIVEQLDSTIVIPPDVTAEVDEWLNLRIAVG"
     gene            2899..4188
                     /locus_tag="VHF88_07840"
     CDS             2899..4188
                     /locus_tag="VHF88_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2161717.1"
                     /translation="MKQQETRFWHPFANMAEVAGHEFTVTRAKGVHLWDDAGRRHLDA
                     SASLWCANVGHGRPEIAAAVHAQLLELDAYMTFGDYTNPQAEELCRRLAELAPVPGAR
                     VFLGSGGGDAIEMAAKLARQYWALEGMPRRQHVLGRVGGYHGTHGFGTSIGGIEANRA
                     AFGPLVAATGQVDRDDLGALERRILELGPDSVAAFVCEPVLGAGGVRLPEPGYIEGAA
                     ALCAEYGVLFVVDSVICAFGRLGTWFGIERWDGVEPDMITFAKGVSNGALPLGGTIVS
                     ERVAEPFWEGEGSMFRHGQTYSGHSACCVAALANIEVLEQENLIPRGQELEGDLHDAL
                     RPLADHPLIAEVRAGLGLLAAVELDAGALAREPGLALRLQRAVRERCVLVRPLPTALA
                     ISPPLTIEREQLQEIADAIDDAVREVAHDIPARSVTG"
     gene            4254..5486
                     /locus_tag="VHF88_07845"
     CDS             4254..5486
                     /locus_tag="VHF88_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2161718.1"
                     /translation="MGALVVALALGACGSDSDDSGGGGGANGGGSDEPIVIGMAIANS
                     GPIAPYDVEAGEAARLRAKQINADGGVNGRPLKMVVKDTRSDKATASNAATELIGEGA
                     VAIIASCDFDYGSPAAIAANAAEVPGISMCASDPQFADKKTIGEYAFSMGVGSDVEGA
                     SNAEWAFNEKDWKSVYILQDESIEYTKALGRYFKARWEQLGGEVVGEDSFPGGENVNV
                     RAQAARIRSEGGGADFIYLPSWNPGAATAIRQLRAAGVDTPILGPAAVDSSALEKIAG
                     PVSDVFFSPYACYVYCAGQDDPGLTEFADAFEAEYGKQPSSSYDLAAYNLVSAIATAA
                     ENADEVTGPALKDQLEQLPEIETPTGEQQFFSPTCHKPIQPTLAYVEVVSGKLRYAGS
                     YQVQEIAGVGDGNPCVSG"
     gene            5483..6277
                     /locus_tag="VHF88_07850"
     CDS             5483..6277
                     /locus_tag="VHF88_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161719.1"
                     /translation="MTEAVSAPPDGAGGRGDAAAPTLRAAGLVMDFQGVRALDGVDLE
                     LAAGEVLGLIGPNGSGKTTCLNVLSGFIVATAGRVGLDAQDVTAWSPARRVRAGLTRT
                     FQGARLFGALTVRENVEVAFCTQRLRGREVERRTDELLLRLGLGQFAETLARSLPGGV
                     ERRLGIARALATEPQFLLLDEPAAGLNEQEGEELVATIRALAADHECGVLLVEHDMRV
                     ITAACDRVQVLDSGRPIARGTPAEVRSDPAVLAAYFGQREADARDS"
     gene            6261..6956
                     /locus_tag="VHF88_07855"
     CDS             6261..6956
                     /locus_tag="VHF88_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161720.1"
                     /translation="MLEIRDLTVRYGRIAAVQGASLHVGEGELVGLVGPNGAGKSSTL
                     KAVAGLVGAADGEVLVAGEPVGSSPIRAGIAYVPESRRIFGGLTVEENLRLGATTRGR
                     SDRSAELRRMYERFPLLGTRRRSGAGDLSGGQQQILAIARALVARPRLLLLDEPTLGL
                     SPAMIDEVFGIVASLREDGVTVLLVEQNTLRTLALADRTYVMRPPGQIVFEGSQAELE
                     RDPTVEQRYLQLL"
     gene            6971..7843
                     /locus_tag="VHF88_07860"
     CDS             6971..7843
                     /locus_tag="VHF88_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2161721.1"
                     /translation="MLQDIVNIVSGGSLYCLITLGLALLVGLMRLMNFAYGELIMVGG
                     FAMYLLRDQPFVVFVGGTVVAVVAVSLLTERIAFRPLRGASPVTLLVASLAVSIMLQN
                     GARMTVGPESRGVPPSDWLQQGVAIGGAQVPRLDLVTIALAAATLAALTLLMRRTELG
                     IQLRAAAEDFEMAGLLGVRANAVIASAFAITGVLAALVTLILIEDQGAVTATVGTGPV
                     LIAFVGVVLGGMRSLVGATLGGFLLGAATTALDVMLPNDLSPFRDAFVFAAVIAVLAF
                     RPEGLVPGRAERLA"
     gene            7840..>8888
                     /locus_tag="VHF88_07865"
     CDS             7840..>8888
                     /locus_tag="VHF88_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2161722.1"
                     /translation="MTGLPLRAVRAVWVPAVLIAALLLAVVLVSANGDLVLERTATLL
                     AINLVIAVAFHVFSGNSGVLSFGHLAFVALGAYVCALLTIPANLKASLFPDMPSFLVW
                     ILDVEMGLVPAALLAGACAAVLALVVAPTLMRLSGLPAGISTLALLVIVYTVLLHWEE
                     VTRGSSTMIGVPPDVKLGGATAVACAAVAIAWAFQRSRAGLRLRASREDEVAAAALGV
                     RVAADRTRAWVLSAFLAGVGGAFYSHFITTFTPSEFYLEATFMIIAMIVVGGLGSLTG
                     TVLGVVLITVLDDILGRMQDGGLFGLSLPAGSAALGVAILLLAMLVKRPDGLTGGREL
                     PLPRAWRRGAPAGAG"
BASE COUNT         1145 a         3253 c         3235 g         1255 t
ORIGIN      
        1 gcctacgccg ccgacgtcga ggacggcggc ggctcgatcg cgctcggctg cgccgtcgag
       61 cggatcgaca gcagcgcaca cgaggcggtc gtccgccacg cccgcggcac gaccgtcgcg
      121 cgccacgcga tcgcctgcgc cggcgcctgg gccggccacc tcgccccatc ggaccagcgc
      181 acccgcttcg tccacttccg cggcgcctac aagcagctga ccgcgcagcg cgccgggctc
      241 gtcaacggcc tcgtctaccc ggtgccggat ccggcgctgc cgttcctcgg catgcacttc
      301 acgcgcaccg tcgacgaccg catgatcctc ggcccgacgg cgctcgccgt cggcgcgccc
      361 gacgcctacc ggatcgcccg catcgctccg cgcgacgccg gccgggcgct cgcctggcct
      421 ggcacctggc ggatggcgcg ccgctggtgg cgcaccgcgc tgcgcgaggt ctcgacggcg
      481 gtcagcaacc gcgccttcct cgccgacgcg cgccgctacg tgcccgcgct cgagcgcggc
      541 gacctcgtcc ccgccggcgc cggagtccgc gcgcagccgg tggcgcgcga cgggtcgctg
      601 gtcgacgact tcctgctctc ggtccagggg cgcgtgctgc acgtcgcgag cgcgccgtcc
      661 ccggctgcga cctcgtcgct gccgctgggc cgtctgatcg tcgagcgcgc ccatgacgcg
      721 ctcggtctcg gcgaccaccg gtcgcgtacg caaccaccac cacccaagga ggctgagcgg
      781 acatgagctc acttcgcgtc gcggtcgacg taggcgggac gttcaccgac atctgcatcc
      841 tcgacgagac ggggggctcg gccctgcgcg tcgccaaggt cccgacgacc gccgatccga
      901 tcgagggcgt gctcgagggc gtccgccacg ccggcgtccc gttcggcgac gtcgtcctgt
      961 tctcgcacgg cacgaccgtc gcgaccaacg cgctgatcac gcgccgcctc ccgccggccg
     1021 cgatggtcac caccaagggc ttccgcgacg tgatcgagat ccgccgcggc aaccgcgacg
     1081 acctctggga cgcctacaag gacatcgcgc cgccgcacat ccgccgccgc gaccggctcg
     1141 aggtcagcga gcggatcgac tatgccggcg aggtcgtcga gccgctcgac gaggacgagg
     1201 cccgccacgt cgcgcgcgtg ctgcgcaagc gggaggtggc cgcggtcgcc gtctgcctga
     1261 tcaacgcgca cgcgaacccc gagcacgagc agcggctggc ggagatcctc gcggaggagc
     1321 tgcccggcgt ctggatctcg acgtcgagcg acgtgctgcc cgagctgttc gagcacgagc
     1381 gcttctcgac gaccgtctcg aacgccgtcc tgtcgccgct ggtcagcaac tacgtgtcac
     1441 ggctcgacgc cgggctgcgc gaaggcggct acaagggcga cctcgtgctg ctgcactccg
     1501 gcggcggcgt gatgaccgcc cagacctcgg agcgctacgc cgcgcggctg gccgcgtccg
     1561 gcctcgccgc gggcgcgatc gccagccgcc acatcgcgac gatgtgcggc ttcgacaacg
     1621 cgatcggcat cgacatgggc gggaccagca ccgacatctc gctggtctac gacggcgagg
     1681 cgcgcgtcac caaggagtgg ttcgtcgagt tcggctaccc gatcctcttt ccgagcatcg
     1741 aggtgctgac gatcggcgcc ggcgggggct cgctggcctg gatcgacgag gcgggctcgc
     1801 tgcacagcgg gccgcagtcg gccggcgcga tcccggggcc ggctgcctac gggcgcggcg
     1861 gcgaggagcc gacgacgacc gacgccaacc tcgtcctcgg ccggctcggc ggcgagctga
     1921 tcggcggcga gatgacgctc gaccgcaagc tcgcgagcga ggcgctgcgc accaaggtgg
     1981 ccgagccgct cgggctcgat gtcgacgacg cggcgcgtgc tgtgacgcgc gtcgcaaacg
     2041 cgaacatggc cgacgcgctg cgcctgatct cgatccggcg cggttacgac ccgcgcgagt
     2101 tctgcctcgt cgtcttcggc ggcgcaggac cgctgcacgg cgtcgagctg gcgcgcgagc
     2161 tgtcgatccc gaccgtcctg gtgccgctcg acccggggat cacctcggcc ttcggctgcc
     2221 tgctggtcga cgtccgccac gacctgacgc agatgtacct gaccgacgcc ggcagcgccg
     2281 accccgagga tgtcgaggcg agcttccagg cgctcgagga gcagggccgc gagcagctcg
     2341 acaccgaggg cgtcccgcgc gagcagcagg agctcgtgcg cacgatcgac atgcgctacc
     2401 gcggccagtg gcgctcgctc agcgtcgcgg tcggccgccc gctgcgctcg ctcgatgacg
     2461 cgatcgcgac cttccacgcc gagcacgagc gggagtacaa cttccgccgc gacgacacgc
     2521 cggtcgagat ctaccggctc ggcatcgggg cgatcggcat cacgccgaag cccgatctgc
     2581 cgcgccacga gctcagcgac gcgccggtcg agccggtcga gcgccgcatg gtcactttcg
     2641 acggcgtcga ggagccgcgc gaggcggccg tctacctgcg ctcggacctg cacgccgggg
     2701 cgcacgtcga ggggccggcg atcgtcgagc agctcgactc gacgatcgtg atcccgcccg
     2761 acgtgacggc cgaggtcgac gagtggctga acctgcggat cgcggtcgga tgagccctcg
     2821 tagcgggcac caagcggcgg tcccgattca ggagatcgac caatgtcgcc cgcatcgggg
     2881 cgctggaagc ctgatcccat gaagcagcag gagacgcgct tctggcaccc cttcgccaac
     2941 atggcggagg tcgccggcca cgagttcacc gtgacccgtg ccaagggcgt ccacctttgg
     3001 gacgacgccg gccgtcgcca cctcgacgcg tcggcgagcc tctggtgcgc gaacgtcggc
     3061 cacggccggc cggagatcgc ggccgccgtc cacgcgcagc tgctcgagct cgacgcctac
     3121 atgacgttcg gggactacac gaacccccag gccgaggagc tctgtcgccg gctcgccgag
     3181 ctggcgcccg tccccggcgc gcgcgtcttc ctcggcagcg gcggcggcga cgcgatcgag
     3241 atggcggcca agctggcccg ccagtactgg gcgctcgaag ggatgccgcg gcgccagcac
     3301 gtgctcgggc gcgtcggcgg ctaccacggc acgcacggct tcggcacgag catcggcggg
     3361 atcgaggcga accgggccgc gttcggcccg ttggtggcgg cgaccggcca ggtcgaccgc
     3421 gacgacctgg gcgcgctcga acggcggatc ctcgagctcg gccccgactc ggtcgccgcc
     3481 ttcgtctgcg agccggtgct cggcgccggc ggcgtgcgcc tgccagagcc cggctacatc
     3541 gagggcgccg cggcgctctg cgcggagtac ggcgtgctgt tcgtcgtcga ctctgtgatc
     3601 tgcgccttcg gccggctcgg cacgtggttc gggatcgagc gctgggacgg cgtcgagccc
     3661 gacatgatca ccttcgccaa gggcgtcagc aacggcgcgc tgccgctcgg cggcacgatt
     3721 gtctccgagc gcgtggccga gcccttctgg gagggcgagg gctcgatgtt ccgccacggc
     3781 cagacctact ccgggcactc ggcctgctgc gtcgccgcgc tggcgaacat cgaggtgctc
     3841 gagcaggaga acttgatccc gcgcggccag gagctcgagg gcgacctcca cgacgccctg
     3901 cgcccgcttg ccgaccaccc gctgatcgcg gaggtccgcg ccgggctcgg gctgctcgcg
     3961 gccgtcgagc tcgacgccgg cgcgctggcg cgcgagccgg ggctcgcgct gcggttgcag
     4021 cgggcggtcc gcgagcggtg cgtgctggtg cgcccgctgc cgacggcgct ggcgatcagc
     4081 ccgccgctga cgatcgagcg ggagcagctt caggagatcg cggacgcgat cgacgacgcg
     4141 gtgcgcgagg tcgcgcacga cattccggcg cgctcggtga ccggatgaac gagatcggct
     4201 gcggccgaga gaaggaggag cggatgaaca gctggagagg tctcatagcc ctcttgggcg
     4261 cactcgtcgt tgccctggcg ctcggcgcct gcggcagcga ctcggacgac tccggtggcg
     4321 gtggcggcgc gaacggcggg ggcagcgacg agccgatcgt gatcggcatg gcgatcgcca
     4381 acagcgggcc gatcgccccg tacgacgtcg aggccggcga ggccgcgcgg ctgcgtgcga
     4441 agcagatcaa cgccgacggc ggcgtcaacg gccggccgct gaagatggtc gtcaaagaca
     4501 cccgctccga caaggcgacc gcgagcaacg cggcgaccga gctgatcggg gagggcgccg
     4561 tcgcgatcat cgcgtcctgc gacttcgact acggcagccc ggcggcgatc gccgccaacg
     4621 cggccgaggt cccgggcatc tcgatgtgcg cctcggaccc gcagttcgcc gacaagaaga
     4681 cgatcggcga gtacgccttc tcgatgggcg tcggctccga cgtcgagggc gcgtcgaacg
     4741 ccgagtgggc gttcaacgag aaggactgga agtcggtcta catcctccag gacgagagca
     4801 tcgagtacac caaggcgctc ggccgctact tcaaggcgcg ctgggagcag ctcggcggcg
     4861 aggtcgtcgg cgaggacagc ttcccgggcg gcgagaacgt caacgtgcgt gcccaggcgg
     4921 cgcggatccg ctccgagggc ggcggcgccg acttcatcta cctgccctcg tggaacccgg
     4981 gtgccgcgac ggcgatccgc cagctgcgcg cggcgggcgt cgacacgccg atcctcgggc
     5041 cggcggccgt cgacagctcg gcgctcgaga agatcgccgg gccggtcagc gacgtgttct
     5101 tcagccccta cgcgtgctac gtgtactgcg cggggcagga cgatccgggc ctgaccgagt
     5161 tcgccgacgc gttcgaggcc gagtacggca agcagccgtc ctcgtcctac gacctggcgg
     5221 cgtacaacct ggtctcggcg atcgccacgg cggctgagaa cgccgacgag gtcaccgggc
     5281 ccgcgctcaa ggaccagctc gagcagctgc ccgagatcga gacgccgacc ggcgagcagc
     5341 agttcttctc gccgacctgc cacaagccga tccagccgac gctggcctac gttgaggtgg
     5401 tcagcggcaa gctgcgctac gccggcagct accaggtgca ggagatcgcc ggagtcggcg
     5461 acggcaatcc ctgtgtgagc gggtgaccga ggcggtctca gctcctcctg acggcgctgg
     5521 gggccgcggc gacgccgcgg cccccacgct ccgcgcggcc ggcctggtga tggatttcca
     5581 gggcgtgcgg gcgctcgacg gcgtcgacct cgagctggcc gccggcgagg tgctgggcct
     5641 gatcgggccg aacggctcgg gcaagacgac ctgcctcaac gtgctcagcg gcttcatcgt
     5701 cgcgaccgcc ggaagggtcg ggctcgacgc gcaggacgtc accgcgtggt cgcccgcgcg
     5761 gcgcgtccgc gctgggctga ccaggacgtt ccagggggcg cggctgttcg gcgccctgac
     5821 cgtgcgcgag aacgtggagg tcgccttctg cacccagcgg ctgaggggcc gtgaggtcga
     5881 gcggcgcacc gacgagctgc tgctgcggct cgggctcggc cagttcgccg agacgctcgc
     5941 gcgatcgctg ccgggcggcg tcgagcgccg gctcgggatc gcgcgcgcgc tcgcgaccga
     6001 gccgcagttc ctgctgctcg acgagccggc cgccgggctc aacgagcagg agggggagga
     6061 gctcgtcgcg acgatccgcg cgctcgccgc cgaccacgaa tgcggcgtgc tgctggtcga
     6121 gcacgacatg cgcgtgatca cggcggcctg cgaccgggtg caggtgctcg actcgggccg
     6181 gccgatcgcg cgcggcacgc cggccgaggt ccgctccgac ccggcggtcc tggcggccta
     6241 cttcggacag cgggaggccg atgctcgaga ttcgtgacct gacggtgcgc tacgggcgca
     6301 tcgccgccgt ccagggcgcg tcgctgcacg tcggcgaggg cgagctcgtc ggcctcgtcg
     6361 gcccgaacgg cgccggcaag tcgagcacgc tgaaggccgt ggcgggcctc gtcggcgcgg
     6421 ccgacggcga ggtgcttgtc gccggcgagc cggtcggctc gagcccgatc cgcgccggca
     6481 tcgcctacgt ccccgagagc cggcggatct tcggcggcct gaccgtcgag gagaacctgc
     6541 gcctcggcgc gacgacgcgc gggcgctccg atcggtcggc cgagctgcgg cggatgtacg
     6601 agcgcttccc gctgctgggg acgcgccgcc gctccggggc gggcgacctc tccggcggcc
     6661 agcagcagat cctcgcgatc gcccgcgcgc tggtcgctcg cccgcgcctg ctgctgctcg
     6721 acgagccgac gctcggcctg tcgccggcga tgatcgacga ggtcttcggg atcgtcgcga
     6781 gcctgcgcga ggacggggtc acggtcctgc tcgtcgagca gaacacgctg cgcacgctcg
     6841 cgctcgccga ccgcacctac gtgatgcgcc cgcccggcca gatcgtcttc gagggctcgc
     6901 aggccgagct cgaacgcgac ccaacggtcg agcagcgcta cctgcagctg ctctgaggag
     6961 aggaggcccc ttgctccagg acatcgtcaa catcgtcagc ggggggagcc tctactgcct
     7021 gatcacgctc ggcctggcgc tgctcgtcgg gctgatgcgg ctgatgaact tcgcctacgg
     7081 cgagctgatc atggtcggcg gcttcgcgat gtacctgctg cgcgaccagc cgttcgtcgt
     7141 cttcgtcggc ggcaccgtgg tagcggtcgt tgccgtctcg ctgctgaccg aacggatcgc
     7201 cttccggccg ttgcgcgggg cgtcgccggt gacgctgctg gtcgcctcgc tcgccgtcag
     7261 catcatgctc cagaacggcg cgcggatgac cgtcgggccg gagtcgcgcg gcgtgccgcc
     7321 gtcggactgg ctgcagcagg gcgtcgcgat cggcggcgcc caggtcccgc gcctcgacct
     7381 cgtgacgatc gcgctcgccg ccgcgacgct cgccgcgctg accctgctga tgcggcgcac
     7441 cgagctcggc atccagctgc gcgccgcggc cgaggacttc gagatggccg gtctgctcgg
     7501 tgtgcgcgcc aacgcggtga tcgcctcggc cttcgcgatc accggcgtgc tcgccgcgct
     7561 ggtgacgctg atcctgatcg aggaccaggg cgccgtgacg gcgacggtcg gcaccggccc
     7621 ggtgctgatc gccttcgtcg gggtggtgct cggcggcatg cgctcgctcg tcggcgccac
     7681 gctcggcggc ttcctgctcg gcgccgccac caccgcgctc gacgtgatgt tgcccaacga
     7741 cctgtcgccg ttccgcgacg cgttcgtctt cgcggcggtg atcgcggtgc tggcgttcag
     7801 gcccgagggg ctcgttccgg gccgcgcgga gcgactcgca tgaccggcct gccgctgcgc
     7861 gccgtccgcg ccgtctgggt gccggcggtg ctgatcgccg cgctgctgct cgccgtcgtg
     7921 ctggtcagcg cgaacggcga cctggtgctc gagcgcacag cgacgctgct cgcgatcaac
     7981 ctcgtgatcg ccgtcgcctt ccacgtcttc agcggcaact ccggcgtgct gtcgttcggc
     8041 cacctcgcgt tcgtcgcact cggcgcctac gtctgcgcgc tgctgacgat ccccgccaac
     8101 ctcaaggcgt cgctgttccc cgacatgccg agcttcctgg tctggatcct cgacgtcgag
     8161 atggggctgg tgcccgcggc gctgctcgcc ggcgcctgcg cggccgtgct ggcgctggtc
     8221 gtcgcgccga cgctgatgcg gctgtcgggg ctgcccgccg gcatctcgac gctcgcgctg
     8281 ttggtgatcg tctacaccgt cctcctgcac tgggaggagg tcacgcgcgg ctcgagcacg
     8341 atgatcggcg tgccgcccga cgtgaagctc ggcggagcga ccgccgtcgc ctgcgcggcg
     8401 gtcgcgatcg cctgggcctt ccagcgctcg cgcgccgggc tgcgcctgcg cgcgtcgcgc
     8461 gaggacgagg tcgcggcggc ggcactcggc gtccgcgtcg ccgccgaccg cacgcgcgcc
     8521 tgggtcctga gcgccttcct ggccggcgtc ggcggcgcct tctactcgca cttcataact
     8581 accttcacgc cgagcgagtt ctacctcgag gcgacgttca tgatcatcgc gatgatcgtc
     8641 gtcggcggcc tcgggtcgtt gaccggcacc gtgctcggcg tcgtcctgat caccgtcctc
     8701 gacgacatcc tcggacggat gcaggacggc ggcctcttcg gcctgtcgct gcccgcgggc
     8761 tcggccgcgc tcggcgtggc gatcctgctg ctggcgatgc tggtcaagcg gccggacggc
     8821 ctgaccggcg gccgcgagct gccgctgccg cgggcgtggc gccgcggcgc gccggccggc
     8881 gcgggtca
//
LOCUS       DATABV010000179        20946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1020711, whole genome shotgun sequence.
ACCESSION   DATABV010000179 DATABV010000000
VERSION     DATABV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20946
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020711"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..355
                     /locus_tag="VHF88_07870"
     CDS             113..355
                     /locus_tag="VHF88_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161723.1"
                     /translation="MVEFLVAIAGLAAASAPVTSFGEDAATAVGAGAFLGGFTAGVIG
                     VLLGRKETWRDRAAINGSYVGGAAMVVAMAGERLIG"
     gene            361..573
                     /locus_tag="VHF88_07875"
     CDS             361..573
                     /locus_tag="VHF88_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161724.1"
                     /translation="MQMSLRNGNIGLAVAVAMMVIGALIVGNGASDMEMQLIFWPAFL
                     TGIAVMLILEGREERRQRRDRSPRGR"
     gene            complement(628..1302)
                     /locus_tag="VHF88_07880"
     CDS             complement(628..1302)
                     /locus_tag="VHF88_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX2161725.1"
                     /translation="MGKRSYGEACGIPRALDRIGERWALMVVRELLLGPKRFTDLREG
                     LPHMSADVLAQRLRELEENGIVERRVLPPPAASKVYELTEWGRELESVLIALGRWGAR
                     APLTSECSEMSLDSHIVSLQTLFDPSLAGDFAPVVELRLGEHVFHVQPVDGRLDAGRG
                     PADAPDVVIATNPCTLLALSHRQRELADAVAAGDAKVTGDKRAARRFLRLFPLPEPAC
                     PQEAVA"
     gene            1429..2937
                     /locus_tag="VHF88_07885"
     CDS             1429..2937
                     /locus_tag="VHF88_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2161726.1"
                     /translation="MTAAADTPTDDRKRWLALYVLCAGALMIVLDATIVNVALPTIQD
                     DLGFSQSNLAWVVNAYLIPFGGLLLFAGRLGDLIGQRKIFLIGLGVFTVASALCALAQ
                     NQEMLIAARFLQGAGGAATAAVVLGMIVTMFPEPREQAKAIGVYGFVASAGGSIGLLA
                     GGVLTDVISWHWIFFINIPVGIATAYFALRLVDGDEGIGFAQGADIPGAVLLTAGVML
                     AVYAILGVSEDGWVSGRVLGLGAVGLALVAAFVLRQKRLEHPLMPLRLFESRNVVGAN
                     LVMVFLVAGMFSMFFLGALYLQRVLGYDPLEVGLAFLPSTVVMGTMSLRYSEKLIMRF
                     GPKSTLVASLTFFALGLLLFARTPVDGSYLLDVVPPVLFIGLGAGMGFPSLMMLSMSG
                     VQPQDSGLASGLINTTVQVGGAIGLAVLATLATDRTEGLLAAGESAISALNSGFHLAY
                     VIGAALVAIAFAVAITVLRDPSPAELAAAHGGGGPEDGAHASEPEPALAEIG"
     gene            3056..4267
                     /locus_tag="VHF88_07890"
     CDS             3056..4267
                     /locus_tag="VHF88_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2161727.1"
                     /translation="MRARKREVEQLEGSLAEARRKIAALEEELRAQRTSDSLTGLPVL
                     GELTRRLGQEIERSRRHGQPLSIAVLDVDRFRGINAHHGRATGDRVLAAVGALLAEQI
                     RASDAASRSGADEFVVMLPETTAAEALQAFERYFLEFEALRVDDVEGVSVSVGIAQWE
                     RGATAEQLLATAGSRIDVARSLGGGRAAASDAADTADDRGPHAQGDVIAALAEALLER
                     DRYTGEHSESVVDLVESVARGLALSAAEVDHVKAAALLHDIGKVAIPDDVLNKQGPLD
                     DDEWKVMREHPVIGERILRAIPGMGPIARIVRHEHERFDGAGYPDGVSGEAIPIGARI
                     ILACDAYHAMTSDRPYRKGMSHAEAVRELSQHAGSQFDPRVTEVLIGALYGRRQSGGG
                     RTSVESDVAAA"
     gene            complement(4275..4760)
                     /locus_tag="VHF88_07895"
     CDS             complement(4275..4760)
                     /locus_tag="VHF88_07895"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX2161728.1"
                     /translation="MGQDPDEIRQGIEDTRARMGDTVEALGHKTDVTGRAKGAVSDRV
                     ESVKSKVTGTTSKVSDSTPDGQQVKQGAQRAAGMAQENPLGLAIGSVAVGFVAGMLMP
                     STQMEDERIGPMADQMKEKVKETGQEALERGKDVAQQAADSARETAQEAGQEHAEQMR
                     S"
     gene            complement(4724..5146)
                     /locus_tag="VHF88_07900"
     CDS             complement(4724..5146)
                     /locus_tag="VHF88_07900"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2161729.1"
                     /translation="MAERNGDNDLYDRPTGELLRELSQQTTDLVRQEIELAKAEIGEK
                     GKQAGIGAGMFGGAGLFGIGAFAALTTAIIAALALAMDTWIAALIVAVVYGIVAAVLA
                     QRGKQTMQEVGAPVPEQATDSVKEDVQWAKTRMRSGRE"
     gene            complement(5149..5763)
                     /locus_tag="VHF88_07905"
     CDS             complement(5149..5763)
                     /locus_tag="VHF88_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161730.1"
                     /translation="MADTALSAGGTGSSGASNGGATEQAQEKAKEVAGQAQEQVRGRV
                     SEQVDQRSTQAGEQVRGTAEDVRSFAQQLREQGKDKPAQYAEQAADRAERLGGYLQDA
                     DGDRILHDVEDFGRRNPWAVVAGGVALGLVASRMLKASSSQRYRSSMQGSGGGTQSLP
                     AGGTTASGIGGGATGAAPAGTPGLAEDAVHSGTGTKPSQFQPHP"
     gene            complement(5887..6096)
                     /locus_tag="VHF88_07910"
     CDS             complement(5887..6096)
                     /locus_tag="VHF88_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161731.1"
                     /translation="MERPSQGVQLLGSLLAALAIAAVAIALVTAHFGPTSSAERELQE
                     QRLEQREEALEERRELREERLEQGR"
     gene            complement(6113..6556)
                     /locus_tag="VHF88_07915"
     CDS             complement(6113..6556)
                     /locus_tag="VHF88_07915"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HEX2161732.1"
                     /translation="MTGTLEQLIEDVTRGNVTEVKVVLATVVMALAVYQLLLAAVGYR
                     RLRVPFLASAPALWAHRASGDTIALLTVVVALMCVGYYGFDFGDDGGTHAVVAVGLLG
                     VLAAKVLAVRAGGRLGRLLPLLGVSLLVLIGVTWWTSAGGFLGVA"
     gene            complement(6592..7116)
                     /locus_tag="VHF88_07920"
     CDS             complement(6592..7116)
                     /locus_tag="VHF88_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2161733.1"
                     /translation="MARSSLDQMNCSIARTLDVIGDAWTPLILRDIAFGITRFDAIQR
                     NLGISRKVLSERLGALVEHGVLQRVPYQDKPARYDYHPTEKGADLAFVLLAMQAWGDR
                     WTASDDGPPILLRHETCGALTKVVPACASCGERLAVTDVTPLRGPGFETGPGNAEISA
                     VLERREAAATAADA"
     gene            7198..9333
                     /locus_tag="VHF88_07925"
     CDS             7198..9333
                     /locus_tag="VHF88_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2161734.1"
                     /translation="MLSRIADLTWTRPKLVLALVGAFAVLAVAIGHDVEKHLKAAGFT
                     DSASESERATDRLRDALGYDPNPGLVLVVRDPDGGKLDVRSPAVQREVTRLSEEVAKV
                     EGVGHVIDPLRERRAGARLIAEDGESLIVSGYLSAVDVEDEGGRIAERVAPIAASSES
                     LDVSLTGFAPGFNDVNDQTREDLTKAELIAFPILAILLLLVFRGVIAAAIPLLIGVLS
                     ILGAFLLLRLMSGFVDTSLFALNIATGLSLGLAVDYALLMVSRYREELGRDGPTREAH
                     RRTVMTAGRTALFSGFTVAVALAALGVMPQRFLYSMAFAGAAVGILSAVIAILVVPSL
                     LALLGTNIDKWSIRRGPAVSDASGGWYRLAHAVMRRPVATALASAALLLAAASPLLFT
                     VLTGPSAEAVPAGVPSYDPGVYVEDHYPRDINEAITVTVDGRASDEQLAAFQRRIAAI
                     DGLEPATPFVRASRAVAYANFAADGPALAQRSQDAVDQLRELAPPSATAALVSGNTAR
                     FIDQKQSIVDHLPLVVGIVALTTLAILFMLTGSVLLPIKTLLMNALTLGATLGVIVLA
                     FQEGWLEGLFDYAGPEAIEVTSLVFLFAVIFGLATDYAVLVMARIKELHDLGYSNEEA
                     VATGIGRTGRVITAAAVMIAVVFLAFSVSPVFFMKQIALGTAFGVLVDATIVRALLVP
                     SLMRLFGDWNWWAPGPLRRLQLRYGFREA"
     gene            9520..10560
                     /locus_tag="VHF88_07930"
     CDS             9520..10560
                     /locus_tag="VHF88_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX2161735.1"
                     /translation="MPPVRTPAVTAAVVAASLVAIGFATATAQEPPDTTAPPAADEPV
                     QPAHQRLWMAAPRRSAVGRQFEMVVRRAARNREGRYVAKLCVAAPALGWTCNRLALRS
                     GVRRLERRLTAEYPGRWLFEIRNGKQRIRRTVNVAPRGGRLKVLATGDSMTQIFDHYL
                     ADGLGGRASVRSEAHISTGLSKPSMLDWVAHSRAQARRVKPDVTVVFIGANDGFPMST
                     PSGATAPCCGDAWVKEYARRAAEMIRAYRRGGVGRVYWLTLPAPRGGSFRTVFGPVNA
                     AIRRAARRTRGAKVVPIDRIFTPGFVYRDSMMWGGRNVTVRQGDGVHLNNAGASIAAQ
                     KTIARMRANRIF"
     gene            10565..11959
                     /locus_tag="VHF88_07935"
     CDS             10565..11959
                     /locus_tag="VHF88_07935"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX2161736.1"
                     /translation="MVFPSVEFAIFFPLVLAASWALMPWPRVWKPFILAASYAFYAAA
                     SPRYCLLLAGITLANQLGAVLVHRTADERRRRWIVGLTVAFDLAVLGVFKYYGFFADE
                     IGGFLDGIGLGISPPLLTLALPIGLSFITFQAISYTVDVKRGLLAPGSTIDVAIYLSF
                     FPHVVAGPIVRAREFLPQLRVPRDPRNVAVGAGVVLIMLGLIKKVALADLLAREIVDP
                     VFGVPQAYAAPDVALAAYAYAAQIYCDFSGYTDIAIGVALLCGFVFPQNFNSPYRATS
                     FGDFWRRWHITLSRFLRDFLYIPLGGNRGGRWTTARNLMITMVLGGLWHGAAWGFVLW
                     GAIHGLALVVEHQLRRTRLSLPAWARWAIVFNVVVFAWVLFRAENLALAGDFLGQLFV
                     SGPATLWTAPVVGAVLVVIGFQLLPEDPLQRLRIRVANLPAPALGVALAAIVAVVGAT
                     VPGEGVPPFIYFQF"
     gene            11965..12966
                     /locus_tag="VHF88_07940"
     CDS             11965..12966
                     /locus_tag="VHF88_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX2161737.1"
                     /translation="MTEPTTATTASDPVAAAGGPADPTGGELPHDVFAVPDPPPGRRG
                     RARDALIVVGIAVLLLVAFQGAAVRGGAEELSPGIGRDVVEAVGEPTGWIADRLPFSG
                     PTDDALAWLESDGEPEAAPVAGGGDVTLLVTGDSLAMPLDTELARRYAGSEVAVERDP
                     HVGTGISKSDLVDWTELSAKQARRHDPDAVVVFIGANEGFSLPAPGGRQVNCCGAPWQ
                     AAFAARVREIADAYLAEGDARVYWLTVPKPRDPARQEIADGVNAAVASAAEQAGDRVR
                     VIDLDETFAPDGDYTDAIDVDGRERIVREQDGIHLNAAGAAVAADLVQQAVDDDFAR"
     gene            complement(12959..13342)
                     /locus_tag="VHF88_07945"
     CDS             complement(12959..13342)
                     /locus_tag="VHF88_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161738.1"
                     /translation="MLRRTPLLAVVATLGALAVLPAGAQAASGPQAVAAKSCSLAGKT
                     RELGPTYTTSLSVRRVSCSKGESLVKAYYKCRKRNGGKRGRCGGVSGYSCGERRYDSI
                     PTQFSATATCKKGRKKVVHKYTQFT"
     gene            13436..14260
                     /locus_tag="VHF88_07950"
     CDS             13436..14260
                     /locus_tag="VHF88_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX2161739.1"
                     /translation="MEEGPGQSRLIDRFRQARRDDGWAVLEGFHALKHALRFEGAVEA
                     VLTSEPAELERLAAELAPDVGARLRELATAVPEELYRELGPYAHHTRVAGLARRPRFE
                     AAAVLRERRDAPVVLLEDPRHLGNLGAVVRVAAAADAAGVLTTGDQDPWDPVAIRGSG
                     GLHFALPVGRIAALDGADRAGRPLLAIDPEGEQIDPRTLPADAIYAFGSERNGLSDAA
                     IAAADRHLRIPMRPGVSSLNLATSVSAVLFAWRLTGGDGVGAAGTALGEDETGHQQ"
     gene            complement(14347..15174)
                     /locus_tag="VHF88_07955"
     CDS             complement(14347..15174)
                     /locus_tag="VHF88_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2161740.1"
                     /translation="MDSQDRLLLGPRRNPTHSPRSRDRTAEALRRRIRDLETRNAELV
                     EFAHFTAHDLKEPLSTVGLFADTLDSLQGETLDDRGRRMLHGIRDGIDQMRRTIDGAL
                     AGVERRRDEDEQVDMGAVVDRALRAVEWRLERGSVRVKVGEMPTVTGSRAQLTRLMQN
                     LLANALEFAEGAQRAWIAVEAQRQPDSWLFQVSDNGPGVSTENPERLFERYERGDGRG
                     SGLGLGLTICRAVVEQHGGRISIQARPGGGTVVSFTLPATSSRRLAGTRPVEGLRGA"
     gene            15307..15960
                     /locus_tag="VHF88_07960"
     CDS             15307..15960
                     /locus_tag="VHF88_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2161741.1"
                     /translation="MEALQDKVRVLLVDDFALMRKGVTAALESSGAMTVVGEAGTVEE
                     AVALAERLQPDVVVLDMRLPGGGGLVAIDRLREIAPGARVLVLTASDKPETLIEAVSA
                     GATGYLTKRAGGEELCAAVAQVATGASVVEPSLAGHLMRELAGERDVGGSARVSLTGR
                     ERDVMRLVCEGLTDREIGERLFVSTRTVQNDLRRLRDKAGLERRSELVHWATSRSLA"
     gene            15968..16621
                     /locus_tag="VHF88_07965"
     CDS             15968..16621
                     /locus_tag="VHF88_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2161742.1"
                     /translation="MKIRVLVADDFPLLLDGLCTALDADPAFDVVGRAADGVEAATRA
                     AELSPDIVLLDMMMPRCDGPAAIELVRSHAPGARVIAISASERIELVRDALDAGAAGY
                     VSKASLAHELRDAVIHVHGSRNGPYVSPSIADRVLRSESDRPGPGDAGRSRAALLSPR
                     EHDVLRLIAQGYSDSAIADELSIRIRTVQNHLARIRVKTRLRRRPELVTWAMRHGVR"
     gene            16745..18691
                     /locus_tag="VHF88_07970"
     CDS             16745..18691
                     /locus_tag="VHF88_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161743.1"
                     /translation="MTTRRLLTQLPAAALAALALAAPAATASSSTEILVPSGSGITAP
                     SGAALTSDGALWIGDGEKGVCRVRLTQPRGLVRDGVWCDPDTPALDKTGQLAFDSETN
                     FLYVAEADSAAGGIWRLRWDPGTGTIVSGGKIIDTPDDLITGLAVSYTGPPRYEGGPL
                     TGELNFTSKRTNSVRRVREPDGAQPTVVTVGQRRFFGEEDISSIAALGPDLYLAEPFG
                     VTRLTTFPGETPRAELVPGLAEPFPAGVASAVAADPQRGRIYAGTDNGNARDQVDVLD
                     VAGLSQELYDVGVAAVIAIVVEDDGDLLIVDDPSDAAGADVNNQARVWRMRLAPLGRP
                     RATWTQVPPLFTSATNAGFGYTARAGSSFECSIDSAPWQPCGGPTSGYYPMSDLAEGT
                     YVFRVRPVDADPSIGVGRAITRPLVVDRTRPTVSIDNTADDRDVYSNEFKIRFSGSEP
                     GLTFRCSLDGAPAIACTPAYHSFEGLTHGYHTVEVTATDAAGNDSLSGPHARYHFYVH
                     APSPPVRPAPLPPVPPVVAPLLPSPAPAARTAKTVAPKKKKAALRPKAGYARLAGRPR
                     RCARGGFTAYVAGRAIDRVGFRLDGRRIRVVRSANRRGRFAIRVPARRSGPHVLTALI
                     RFDRGAGRSERLALEYRSCPGGLR"
     gene            18688..19377
                     /locus_tag="VHF88_07975"
     CDS             18688..19377
                     /locus_tag="VHF88_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161744.1"
                     /translation="MTFSFTITRARLPLLGLIAAVLALATTGIVLSVVSIAGGSSSGH
                     AHQGPVPNGPQTAGEGIPTSFGAVAVEYAVRLSGLTSKDLAGMTHGVQDYVPPESSQV
                     QVAVTLANMLDTTNEYAPEQFRLIAGTSDVPGPSAKRIVPTGATFKPGTLQPDAAIEG
                     SLSFVVPNKRAHLWVEFHDPERAEPLLVDLGKPAATLPGAPGRADRKRPELPAPPTSF
                     DGTTPHAHPGQ"
     gene            19396..>20946
                     /locus_tag="VHF88_07980"
     CDS             19396..>20946
                     /locus_tag="VHF88_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161745.1"
                     /translation="MTETILRSIARRPASALARPFAVAVYAIVLSLGGVLWLKLVHAL
                     QGSHEPGNTGFLLELGRDFVLALPLQFAWIWIAVLMTRRALQRSGEQSPALSATVLAV
                     VVALVASLALALGAPLHAAIFGAIPAPSHEMPALVHLFWETVVGLVATLPLAAIATAF
                     LLRRRPWDAPDTRRWLALAPSAQRVRLASLLLVLVLAPAATFAGSGVDGATADLSPGS
                     PCPAGAPTKTYDVRAIDVDVPLNRFGDHDPNGKMFVLADRVDDVREQEASQHVSIGLR
                     DDAIQPLVIRANLGDCVEIEFANEASGGEYGVHIDGLSFDMGSSGDAVGNNAPSAAAR
                     GESRTYRYYVPEEPELEGAHYMRPGPGNRQAVAHGLFGTLLVEPPGSEYLDMDTGEPI
                     DSGWEAMIVPGDGKTAFREYAIIQHEIGDESADVFDRAGERLPVVDPLTDGYRPGSRA
                     MNYRSEPFMRRLERNGRMKSHAYGSYAFGDPATPMPRSYQGDPTKMRILHGGSETFHV
                     YHLHGGAIR"
BASE COUNT         2711 a         7893 c         7206 g         3136 t
ORIGIN      
        1 ggggcacgcg cggcgcgtcc ccggcgacgg cataggcggc agcggcgaca ccgttgcact
       61 gctgtcgcag aagtgctgta tccccggagt cgggtaggtc taggttgcct gcatggtgga
      121 gttcctcgtc gccatcgcag gtctcgccgc cgcgagcgct cctgtcacgt cgttcggcga
      181 ggacgcggcg accgcggtcg gtgccggtgc gttcctcggc ggcttcaccg ctggtgtcat
      241 cggggtgctc ctcggccgga aggagacctg gcgtgatcgc gccgcgatca acgggagcta
      301 cgtaggcggc gccgcgatgg tcgtcgctat ggcgggcgag aggctcatcg gatagcgtat
      361 atgcagatga gccttagaaa cggaaacatc ggactggctg tagctgttgc gatgatggtg
      421 attggtgcgc tgatcgttgg gaacggcgcc agcgacatgg aaatgcagct gatcttttgg
      481 ccggcgttcc tgaccggaat cgccgtgatg ctcatcctgg aggggcgaga ggagcgccgc
      541 cagcgtcgcg accggtcacc tcgcgggaga tgactttcta ggaaagccgg ccggctcgca
      601 tccgcgagaa ggcgaggcca acgccgccta ggcgacggcc tcctgcgggc aggccggctc
      661 cggcagcggg aagagccgta ggaagcggcg ggcggcgcgc ttgtcaccgg tgaccttggc
      721 gtcgccggcg gctaccgcgt ccgccagctc gcgctggcgg tgggagaggg cgagcagggt
      781 gcaggggttg gtggcgatca cgacgtcggg cgcgtcggcc gggccgcggc cggcgtcgag
      841 ccgcccgtcg accggctgga cgtggaagac gtgctcgccg aggcgcagct cgaccaccgg
      901 cgcgaagtcg cccgccagcg acgggtcgaa cagcgtctgc aacgagacga tgtgcgagtc
      961 gaggctcatc tccgagcact cggacgtcag cggggcgcgc gccccccagc gcccgagcgc
     1021 gatcagcacg ctctccagct cgcgacccca ctccgtcagc tcgtagacct tcgacgcggc
     1081 cggcggcggc agcacccgcc gctcgacgat gccgttctcc tcgagctcgc gcagccgttg
     1141 ggcgaggacg tcggcgctca tgtgcggcag cccctcgcgc aggtcggtga agcgcttggg
     1201 cccgagcagc agctcgcgga ccaccatcaa cgcccagcgc tcgccgatcc ggtcgagcgc
     1261 tcgggggatg ccacaggcct cgccgtagct gcgcttgccc atgcccgtca gtctagccgt
     1321 cgttgggaaa ttcgtccagt tagttggatt ttccaaccag atggtcggaa gatgtgctag
     1381 aacaccgcca cccgacgacg aaggcaaccc gacacgagag gacctgacgt gaccgcagca
     1441 gccgacaccc ccacagacga ccgcaagcgc tggctggcgc tctacgtgct ctgcgcgggc
     1501 gcgttgatga tcgtgctcga cgcgacgatc gtgaacgtgg cgctgcccac gatccaggac
     1561 gacctcggct tctcgcagtc caacctcgcc tgggtcgtca acgcctacct gatcccgttc
     1621 ggcggactgc tgctgttcgc cggccggctc ggcgacctga tcggccagcg caagatcttc
     1681 ctgatcggcc tcggcgtctt caccgtcgcc tcggcgctct gcgcgctcgc gcagaaccag
     1741 gagatgctga tcgccgcgcg cttcctccag ggcgcgggcg gggcggcgac cgcagcagtc
     1801 gtgctcggga tgatcgtgac gatgttcccc gagccgcgcg agcaggcgaa ggcgatcggc
     1861 gtctacggct tcgtcgcctc ggccggcggc tcgatcggac tgctcgccgg cggcgtgctg
     1921 accgacgtga tctcctggca ctggatcttc ttcatcaaca tcccggtcgg catcgcgacg
     1981 gcttacttcg cgctgcggct ggtcgacggc gacgagggga tcggcttcgc gcagggcgcc
     2041 gacatccccg gggcggtgct gctgaccgcg ggcgtgatgc tcgccgtcta cgcgatcctc
     2101 ggcgtcagcg aggacggctg ggtctcgggc cgcgtactcg gcctcggcgc cgtcggcctg
     2161 gcgctcgtcg ccgcgttcgt gctgcgccag aagcggctcg agcatccgct gatgccgctg
     2221 cggctgttcg agtcgcgcaa cgtcgtcggg gcgaacctcg tgatggtgtt cctggtcgcc
     2281 gggatgttct cgatgttctt cctcggcgcg ctctacctgc agcgggtgct cggctacgac
     2341 ccgctcgagg tcggcctggc cttcctgccc tcgacggtgg tcatgggcac gatgtcgctg
     2401 cgctactcgg agaagctgat catgcgcttc gggccgaagt cgacgctggt cgccagcctg
     2461 acgttcttcg cgctcggcct gctgctgttc gcccgcacgc cggtcgacgg cagctacctg
     2521 ctcgacgtcg tgccaccggt gctgttcatc ggcctcggcg ccggcatggg cttcccgtcg
     2581 ttgatgatgc tgtcgatgtc cggcgtccag ccgcaggact ccggactcgc ctcggggctg
     2641 atcaacacga ccgtccaggt cggcggcgcg atcggcctcg cggtcctcgc gacgctggcg
     2701 accgaccgca ccgagggcct gctcgccgcc ggcgagtcgg cgatctcggc gctgaactcc
     2761 ggcttccacc tcgcctacgt gatcggggcg gcgctggtgg cgatcgcatt cgcggtcgcg
     2821 atcaccgtcc tgcgcgaccc ctcgccggcc gagctggcgg ccgcccacgg cggtggcggc
     2881 ccggaggacg gcgcccacgc gtcggagccc gagcccgcgc tcgccgagat cggctgaccc
     2941 cctgaccgca cgctccgact gagcatttcg gctcagtcgg agcgagctcg ggcggtcagt
     3001 ccaatttcgc ttgcaggcgc tcaagtaagg cgccgtttgg cccgacactg agaccatgcg
     3061 tgcgcgcaag cgagaggtcg agcagctgga gggaagcctc gcggaggcgc gacggaagat
     3121 cgccgcactc gaggaggagc tgcgcgctca gcgcacctcc gattcgctga ccggcctacc
     3181 ggtcctcggc gagctgaccc gccggctcgg ccaggagatc gagcgctcgc gccgccacgg
     3241 ccagccgctg tcgatcgcgg tgctcgacgt cgaccgcttc cgcgggatta acgcccacca
     3301 cggccgcgcc accggcgacc gcgtgctcgc cgcggtcggc gcgctcctcg ccgagcagat
     3361 ccgcgcgagc gacgccgcca gccgctcagg cgccgacgag ttcgtcgtga tgctccccga
     3421 gaccacggcc gccgaggcgc tgcaggcctt cgagcgctac ttcctcgagt tcgaggcgct
     3481 gcgcgtcgac gacgtcgagg gcgtttcggt ctcggtcggg atcgcccaat gggagcgcgg
     3541 ggcgacggcc gagcagctgc tcgccaccgc cggctcgcgc atcgacgtcg cgcggtcgct
     3601 cggcgggggc cgcgccgcgg cgagcgacgc cgccgacacc gccgacgacc gcggcccgca
     3661 cgcccagggc gacgtgatcg ccgcgctcgc cgaggcgctg ctcgagcgcg atcgctacac
     3721 cggcgagcac tccgagtcgg tcgtcgacct cgtcgagtcg gtcgcccgcg gccttgcgct
     3781 cagtgccgcc gaggtcgacc acgtcaaggc ggccgcgctg ctgcacgaca tcggcaaggt
     3841 cgcgatcccc gacgacgtcc tcaacaagca gggcccgctc gacgacgacg agtggaaggt
     3901 gatgcgcgag cacccggtga tcggcgagcg gatcctccgc gccatccccg gcatggggcc
     3961 gatcgcgcgg atcgtccgcc acgagcacga gcgcttcgac ggcgccggct accccgacgg
     4021 ggtctccggc gaggcgatcc cgatcggcgc gcgcatcatc ctggcctgcg acgcctacca
     4081 cgcgatgacc tccgaccgcc cctaccgcaa gggcatgagc cacgccgagg cggtccgcga
     4141 gctgtcccag cacgccggct cgcagttcga cccgcgcgtg accgaggtgc tgatcggcgc
     4201 gctctacgga cgccgccaga gcggcggcgg gcgcacgtcg gtggagtccg acgtggccgc
     4261 cgcctgacgc tcctctagct gcgcatctgc tcggcgtgct cctgacccgc ctcctgggcg
     4321 gtctcgcgtg cgctgtccgc cgcctgctgg gcgacgtcct tgccgcgctc gagcgcctcc
     4381 tggccggtct ccttgacctt ctccttcatc tgatccgcca tcggcccgat ccgctcgtcc
     4441 tccatctggg tggagggcat cagcatcccg gcgacgaagc cgaccgccac cgacccgatc
     4501 gccaggccga gcgggttctc ctgcgccatg ccggccgccc gctgggcgcc ctgcttgacc
     4561 tgctggccgt cgggggtcga gtcggacacc ttcgacgtcg tgccggtcac cttcgacttc
     4621 acggactcga cgcggtccga cacggcgccc ttggcgcggc cggtcacgtc ggtcttgtgg
     4681 ccgagcgcct cgacggtgtc gcccatccgg gcccgcgtgt cctctattcc ctgcctgatc
     4741 tcatccgggt cttggcccat tggacgtcct ccttcacgct gtcggttgct tgctccggca
     4801 ccggggcgcc gacctcctgc atcgtctgct tgccgcgctg cgcgagcacc gcggcgacga
     4861 tcccgtagac gaccgcgacg atcagcgccg cgatccaggt gtccatcgcc agcgcgagcg
     4921 ccgcgatgat cgccgtcgtc agcgccgcga acgcgccgat gccgaacagc ccggcgccgc
     4981 cgaacatgcc ggcgccgatg cccgcctgct tgcccttctc cccgatctcc gccttcgcga
     5041 gctcgatctc ctggcgcacc aggtcggtcg tctgctgcga cagctcgcgc agcagctcgc
     5101 cggtcgggcg gtcgtacagg tcgttgtctc cgttccgctc cgccatggct acgggtgggg
     5161 ctggaactgg ctcggcttgg tgccggtgcc gctgtggacc gcgtcctcgg cgagtccggg
     5221 cgttcccgcc ggggccgcgc cggtcgcacc gccgccgatc ccgctcgcgg tcgtgccgcc
     5281 ggccggaagc gactgggtgc cgccgccgga gccctgcatg ctcgagcggt agcgctggct
     5341 gctcgacgcc ttgagcatcc gcgaagcgac gaggccgagt gccacgccgc cggccacgac
     5401 ggcccagggg ttgcgccggc cgaagtcctc gacgtcgtgc aggatgcggt cgccgtcggc
     5461 gtcctggagg tagccgccga gccgctcggc gcggtcggcc gcctgctcgg cgtactgcgc
     5521 cggcttgtcc ttgccctgct cgcgcagctg ctgggcgaac gagcggacgt cctcggcggt
     5581 gccgcgcacc tgctcgcccg cctgggtcga gcgctggtcc acctgctcgc tgacgcggcc
     5641 gcggacctgc tcctgcgcct ggccggcgac ctccttcgcc ttctcctgtg cctgctcggt
     5701 cgcgcctccg ttggacgcgc cggagctgcc ggtcccaccg gctgaaagag ccgtgtcggc
     5761 catctccact cctcgttgat cgggtgccgt ccgccgtggg tgcccagcgc cggcaaccgc
     5821 aaacgccttc tacggcagat ggcccagaac tgcgacagcc gttcgcgccg cggcggttcc
     5881 agttcgtcag cgaccctgct cgagtcgctc ctcgcgcagc tcgcggcgct cctcgagcgc
     5941 ctcctcgcgc tgctcgaggc gctgctcctg cagctcgcgc tcggcgctgc tggtcggacc
     6001 gaagtgggcg gtgacgagcg cgatcgcgac ggcggcgatt gcgagcgccg cgagcagcga
     6061 tccgagcagc tggacgccct gagacggccg ctccatcagt tgacagcctc cactatgcga
     6121 cccccaggaa cccgccggcg gacgtccacc aggtcacccc gatcaggacc agcagggaga
     6181 cgccgagcag cggcagcagc cggccgagcc ggccgccggc gcgcacggcg agcaccttgg
     6241 cggcgagcac gccgagcagg ccgacggcga cgaccgcatg ggtgccgccg tcgtcgccga
     6301 agtcgaagcc gtaatagccg acgcacatca gcgcgacgac gacagtaagc agcgcgatcg
     6361 tgtcgccgct cgcgcggtgt gcccagaggg cgggcgcgct ggcgaggaac ggcacgcgca
     6421 gccggcggta gccgaccgcg gcgagcagca gctggtacac ggcgagcgcc atgacgaccg
     6481 tcgccaggac gaccttgacc tcggtgacgt tgccgcgcgt gacgtcctcg atcagctgct
     6541 ccaaggtgcc ggtcacctac ccgcgcccgc gcggccgata acggtcaggg gctacgcgtc
     6601 ggccgcggtg gccgcggcct cgcgccgctc gagcacagcg gagatctcgg cgttgccggg
     6661 gcccgtctcg aagcccggtc cgcgcagcgg ggtgacgtcg gtcaccgcga gccgctcgcc
     6721 gcagctggcg cacgcgggga cgaccttggt cagcgcgccg caggtctcat ggcggagcag
     6781 tatcggcggg ccgtcgtcgc tcgccgtcca gcggtcgccc caggcctgca tcgcgagcag
     6841 cacgaaggcg aggtcggcgc ccttctcggt cgggtggtag tcgtagcggg cgggcttgtc
     6901 ctggtagggg acgcgctgca gcaccccgtg ctcgacgagc gcgccgaggc gctcgctgag
     6961 caccttgcgc gagatgccga ggttgcgctg gatcgcgtcg aagcgcgtga tcccgaatgc
     7021 gatgtcgcgc aggatcaacg gcgtccaggc gtcgccgatc acgtcgagcg tgcgcgcgat
     7081 cgagcagttc atctggtcga gcgaggagcg ggccatcccg gctgatgcta gcgatgtcgg
     7141 ttccctggag gaacttttca gttccttttc ggaacggact ctggtacagt cgccgccgtg
     7201 ctgtcgcgga tcgccgacct cacctggact cgcccgaagc tcgtgctcgc cctggtcggc
     7261 gccttcgctg tcctcgcggt cgcgatcggc cacgacgtcg agaagcacct caaggccgcc
     7321 ggcttcaccg acagcgcgtc ggagagcgag cgggcgaccg accggctgcg cgacgcgctc
     7381 ggctacgacc cgaaccccgg gctggtgctg gtcgtccgcg accccgacgg cggcaagctg
     7441 gacgtgcgca gccccgccgt gcagcgcgag gtgacgcgcc tcagcgagga ggtcgcgaag
     7501 gtcgagggcg tcggacacgt gatcgacccc ctgagggagc ggcgcgccgg cgcgcggctg
     7561 atcgcggagg acggcgagtc gctgatcgtc tccggctatc tctcggcggt cgacgtcgag
     7621 gacgagggcg gaagaatcgc ggagcgcgtc gcgccgatcg ccgcttcgag cgagtcgctc
     7681 gacgtctcgc tgaccggctt cgccccggga ttcaacgacg tcaacgacca gacgcgggag
     7741 gacctgacca aggccgagct gatcgccttc ccgatcctcg cgatcctgct gctgctcgtc
     7801 ttccgcggtg tcatcgccgc cgcgatcccg ctgctgatcg gtgtgctgtc gatcctcggc
     7861 gccttcctgc tgctgcgcct gatgtccggc ttcgtcgaca cctcgctgtt cgcgctgaac
     7921 atcgcgaccg gcctcagcct cgggctcgcg gtcgactacg cgctgctcat ggtctcgcgc
     7981 taccgcgagg agctcgggcg cgacggcccg acgcgcgagg cgcatcgccg cactgtgatg
     8041 accgcaggcc gcaccgcgct cttctcgggc ttcacggtcg ccgtcgcgct cgccgcgctc
     8101 ggcgtgatgc cgcagcgctt cctctactcg atggcgttcg ccggcgcggc ggtcgggatc
     8161 ctgtcggcgg tgatcgcgat cctcgtcgtg ccgtcgctgc tggcgctgct cggcacgaac
     8221 atcgacaagt ggtcgatccg ccgcgggccg gcggtatcgg acgcgtccgg cggctggtac
     8281 cggctcgccc acgcggtgat gcgccgaccg gtcgcgaccg cgctggcgag cgcggcgctg
     8341 ctgttggccg ccgcctcgcc gctgctgttc acggtgctga ccgggccgag cgccgaggcg
     8401 gtgccggccg gcgtgccgtc ctacgacccg ggcgtctacg tcgaggacca ctacccgcgc
     8461 gacatcaacg aggcgatcac ggtcactgtc gacggtcgcg ccagcgacga gcagctcgcc
     8521 gcgttccagc gccggatcgc cgcgatcgac gggctcgagc ccgccacgcc gttcgtgcgc
     8581 gcgtcgcgcg ccgtcgccta cgccaacttc gccgccgacg gcccggcgct cgcgcagcga
     8641 tcccaggacg ccgtcgacca gctgcgcgag ctcgcgccgc cgagcgccac ggccgcgctg
     8701 gtctcaggca acaccgcccg cttcatcgac cagaagcaga gcatcgtcga ccacctgccg
     8761 ctggtggtcg ggatcgttgc gctgacgacg ctggcgatcc tgttcatgct caccggctcg
     8821 gtgctgctgc cgatcaagac gctgctgatg aacgccctca cgctcggcgc gacgctcggc
     8881 gtgatcgtgc tggcgttcca ggagggctgg ctcgagggac tgttcgacta cgccggtccc
     8941 gaggcgatcg aggtgaccag cctggtcttc ctgttcgcgg tcatcttcgg cctcgcgacc
     9001 gactacgccg tgctggtcat ggcgcggatc aaggagctgc acgacctcgg ctactcgaac
     9061 gaggaggcgg tcgccaccgg catcggccgc accggccgcg tgatcaccgc cgccgccgtg
     9121 atgatcgccg tcgtcttcct cgccttcagt gtcagcccgg tcttcttcat gaagcagatc
     9181 gcgctcggaa ccgccttcgg cgtgctcgtc gatgccacga tcgtccgcgc cctgctcgtc
     9241 ccctcgctga tgcgcctctt cggcgactgg aactggtggg cgcccggacc cctccgccgc
     9301 ctccagctcc gctacggctt ccgcgaggcc tgaacgcccc cacggattcg ccagatgtcg
     9361 ctttgtgacg tccacgtgat gcagatccga catcgagccc cgacgcgtca atcgcccact
     9421 cgcccgctga tggtttggat cgtccaggcg atgcgaatcc gacatccgtc cccaggtacc
     9481 gtccgccgcg ccggcggttc ggcccgctag tttgcgcgag tgccgcccgt ccggaccccc
     9541 gccgtcactg ccgccgtagt ggccgcgtcg ctggtggcaa tcggcttcgc caccgcgacc
     9601 gcccaggagc cgcccgacac gacggcaccg cctgcggccg acgagccggt ccagcccgcg
     9661 caccagcggc tgtggatggc cgcgccgcgg cgcagcgcgg tcggtcgcca gttcgagatg
     9721 gtggtgcggc gcgcggcgcg caaccgcgag ggccgctacg tcgccaagct ctgcgtcgcc
     9781 gcgccggcgc tcggctggac gtgcaaccga ctggcgctgc ggtccggcgt gcgacggctc
     9841 gagcggcgcc tgaccgccga gtaccccggc cgctggctgt tcgagatccg caacggcaag
     9901 cagcgcatcc ggcgcaccgt gaacgtcgca ccgcgcggcg ggcggctcaa ggtgctcgcg
     9961 accggcgact cgatgaccca gatcttcgac cactacctcg ccgacggcct cggcggccgg
    10021 gcgtcggtgc gcagcgaggc gcacatcagc acgggtctct cgaagccctc gatgctcgat
    10081 tgggtcgccc actcccgcgc gcaggcccgc cgcgtcaagc ccgacgtgac ggtcgtcttc
    10141 atcggcgcca acgacggctt tccgatgtcg actccgtccg gcgcgacagc accctgctgc
    10201 ggcgacgctt gggtgaagga gtacgcccgc cgcgccgccg agatgatccg cgcctaccgc
    10261 cgcggcggcg tcggtcgcgt ctactggctc acgctgccgg cgccgcgcgg tggcagcttc
    10321 cgcaccgtct tcggcccggt caacgcggcg atccgccggg ccgcgcggcg cactcgcggc
    10381 gcgaaggtcg tcccgatcga ccgcatcttc acgcccgggt tcgtctatcg cgactcgatg
    10441 atgtggggcg ggcgcaacgt cacggtgcgc cagggcgacg gcgtccacct caacaacgca
    10501 ggggcatcga tagccgcaca gaagacgatc gccaggatgc gcgccaaccg catcttctag
    10561 gcgcatggtc ttcccctcgg tcgagttcgc catcttcttc ccgctggtgc tggcggcctc
    10621 ctgggcgctg atgccgtggc cgcgcgtttg gaagccgttc atcctcgccg cgagctatgc
    10681 gttctacgcg gcggcgagcc cgcgctactg cctgctgctg gcgggtatca cgctggcgaa
    10741 ccagctcggc gcggtcctgg tccaccgcac cgccgacgaa cggcgccgcc gctggatcgt
    10801 cggcctgacc gtcgcgttcg acctcgccgt gctcggcgtc ttcaagtact acggcttctt
    10861 cgccgacgag atcggcggct tcctcgacgg gatcggcctc ggcatctcgc cgccgctgct
    10921 gacgctggcg ctgccgatcg gcctcagctt catcaccttc caggcgatct cctacaccgt
    10981 cgacgtcaag cgcggcctgc tcgcgcccgg cagcacgatc gacgtcgcga tctacctcag
    11041 cttcttcccg cacgtggtcg ccgggccgat cgtccgcgcg cgcgagttcc tgccgcagct
    11101 gcgcgtaccg cgcgacccgc gcaacgtcgc cgtcggggcg ggggtcgtgc tgatcatgct
    11161 cggactgatc aagaaggtcg cgctcgccga cctgctcgcg cgcgagatcg tcgacccggt
    11221 cttcggcgtc ccgcaggcct acgcggcgcc cgacgtcgcg ctcgccgcgt acgcctacgc
    11281 cgctcaaatc tactgcgact tctccggcta caccgacatc gcgatcggcg tcgcgctgct
    11341 ctgcggcttc gtcttcccgc agaacttcaa cagcccctac cgggcgacca gcttcggcga
    11401 cttctggcgc cgctggcaca tcacgctctc gcgcttcctg cgcgacttcc tctacatccc
    11461 gctcggcggc aaccgcggcg ggcgctggac gacggcacgc aacctgatga tcaccatggt
    11521 cctcggcggg ctctggcacg gggcggcttg gggcttcgtc ctatgggggg cgatccacgg
    11581 gctcgccctg gtcgtcgagc accagctgcg ccgcacgcgg ctgtcgctgc cggcctgggc
    11641 gcgctgggcg atcgtcttca acgtcgtcgt cttcgcgtgg gtcctgttcc gcgccgagaa
    11701 cctggcgctc gcgggcgact tcctcggaca gctcttcgtc tccggcccgg cgacgctgtg
    11761 gaccgcgcca gtcgtcggcg ccgtgctcgt cgtgatcggc ttccagctgc tgccggagga
    11821 tccgctccag cggctgcgca ttcgggtcgc gaacctgccc gcgccggcgc tcggcgtcgc
    11881 gctcgccgcg atcgtcgcgg tcgtcggcgc caccgtcccg ggcgagggcg tgcccccgtt
    11941 catctacttc cagttctgac ccggatgacc gagccgacca ctgcgacaac cgcgtccgat
    12001 cccgtagccg ccgccggcgg tccagcggac ccgaccggcg gcgagctgcc gcacgacgtc
    12061 tttgcggtgc ccgatccacc acccggtcgc cgtggccgcg cgcgcgacgc gttgatcgtc
    12121 gtcggcatcg ccgtgctgct gctcgtcgcc ttccagggcg cggccgttcg gggtggagcg
    12181 gaggagctgt cgccgggcat cggccgcgat gtcgtcgagg cggtgggcga gccgacaggt
    12241 tggatcgccg accgactgcc gttcagcgga ccgaccgacg acgcgctcgc ctggctcgag
    12301 agcgacggcg agcccgaggc ggcgcccgtc gcgggcggcg gtgacgtgac gctgctcgtc
    12361 acgggcgact cgctcgcgat gccgctcgac accgagctgg cccgccgcta cgcgggcagc
    12421 gaggtcgcgg tcgagcgcga cccccacgtc ggcaccggca tctcgaagag cgacctggtc
    12481 gactggaccg agctgtcggc gaagcaggcg cgccgccacg accccgacgc ggtcgtcgtc
    12541 ttcatcggcg ccaacgaggg cttctcgctg ccggcgccgg gcggccgcca ggtgaactgc
    12601 tgcggtgctc cctggcaggc cgcgttcgcc gcccgcgtgc gcgagatcgc cgacgcctac
    12661 ctcgccgagg gcgacgcccg cgtctactgg ctcacggtcc ccaagccgcg cgaccccgcc
    12721 cgccaggaga tcgccgacgg cgtcaacgcg gccgtcgcga gcgccgccga gcaggccggc
    12781 gaccgcgtcc gcgtgatcga cctcgacgag accttcgcgc ccgacggcga ctacaccgac
    12841 gcgatcgacg tcgacggtcg cgagcggatc gtccgcgagc aggacggcat ccacctcaat
    12901 gccgccggcg cggccgtcgc cgccgacctg gtccagcagg ccgtcgacga cgacttcgct
    12961 aggtgaactg cgtgtacttg tggacgacct tcttgcgccc cttcttgcag gtcgccgtgg
    13021 cgctgaactg ggtcgggatc gagtcgtagc gccgctcgcc gcacgagtag ccgctgacgc
    13081 cgccgcagcg gccgcgcttc ccgccgttgc gcttgcggca cttgtagtag gccttgacga
    13141 gcgactcccc cttcgagcag ctcacgcgcc gcacggacag cgaagtggtg taagtcgggc
    13201 cgagctcgcg ggtcttgccg gccaggctgc acgacttcgc ggcgacggcc tgcggcccgc
    13261 tggctgcctg ggcccccgca ggcaggacgg cgagagcgcc gagcgtggcg acgacggcga
    13321 gaaggggggt gcgtcggagc atgtgaggag acgttccttc gtgcgggagc gtggacccgg
    13381 cgatgatgcc acagcaccca cgccgcggcc ggggccgtcg gcataagctg cgcgcgtgga
    13441 ggagggtccc ggccagagcc gcctgatcga ccgttttcgc caggctcgcc gggacgacgg
    13501 ctgggccgtg ctggagggct tccacgcgct caagcatgcg ttgcgcttcg agggcgcggt
    13561 cgaggcggtg ctgaccagcg agccggcgga gctggagcgg ttggcggcgg agctggcgcc
    13621 ggacgtcggc gcgcgtctgc gcgaactcgc gaccgccgtg cccgaggagc tctaccgcga
    13681 gctcggccca tatgcgcacc acacgcgcgt agccggactc gcccgtcggc cgcgcttcga
    13741 ggcggccgcc gtcctgcgcg aacgccgtga cgcaccggtc gtgctgctcg aggacccgcg
    13801 tcacctcggc aacctcggcg cggtcgtgcg cgtggccgcg gcggccgatg ccgcgggggt
    13861 gctgaccact ggcgatcagg acccctggga tcccgtcgcg atccgcggct cgggcggcct
    13921 gcacttcgcg ctgccggtcg ggcggatcgc tgccttggac ggcgccgacc gtgcgggccg
    13981 tccgctgctt gcgatcgacc cggagggcga gcagatcgac ccgcgcaccc ttccggccga
    14041 cgcgatctac gccttcggca gcgagcgcaa tggactgagc gacgccgcga tcgccgctgc
    14101 cgatcgccac ctgcgcatcc cgatgcgccc gggcgtctcg agcctcaacc tcgcgacctc
    14161 ggtgtcagcc gtcctgttcg cgtggcgcct gaccggcggc gatggcgtcg gagcggccgg
    14221 cacggcgctg ggtgaagacg agaccggcca ccagcaatag caccggcagc aggtaggcga
    14281 cggtgctcgc ccagtggaag tggccgccgt gtgcgatcag caggtccatg gcatcggcgc
    14341 tggcgctcag gcgccgcgca acccctcgac cggtcgtgtc ccggcgagtc gacgcgagct
    14401 cgtcgcgggc agggtgaagc tgacgaccgt cccgccgccg ggacgcgcct ggatcgagat
    14461 gcggccgccg tgctgctcga ccaccgcgcg gcagatagtc aggccgagtc cgagcccgct
    14521 gccgcggccg tcgccgcgct cgtagcgctc gaacaggcgc tccggattct ctgtcgagac
    14581 gcccggcccg ttgtcgctga cttggaacag ccagctgtcg ggctggcgct gcgcctcgac
    14641 ggcgatccac gcccgttgcg cgccctcggc aaactcgagc gcgtttgcga gcaggttctg
    14701 catcaggcgc gtcagctggg cgcgactgcc ggtgaccgtc ggcatctcgc cgaccttgac
    14761 gcgcaccgac ccgcgctcga gccgccactc gaccgcgcgc agcgcccggt cgacgacagc
    14821 gcccatgtcg acctgctcgt cctcgtcgcg tcgccgctca actcccgcga gcgcgccgtc
    14881 gatcgtccgg cgcatctgat cgatcccgtc gcggatgccg tgcagcatgc gtcgcccgcg
    14941 atcgtcgagc gtctcgccct gcagcgagtc gagggtgtcg gcgaacaggc cgacggtcga
    15001 cagcggctcc ttgaggtcgt gcgcggtgaa gtgcgcgaac tcgacgagtt ccgcattccg
    15061 cgtctcgagg tcgcggatcc gtcgccgcag cgcctcggcc gtgcggtcac gggaccgggg
    15121 cgagtgcgtg gggttgcgac gggggcccag cagcagtcgg tcttgcgagt ccattcggct
    15181 gcgaacctaa ctcctctttg gcgatatcgc caaagacgtc gcgcaatgca caggttcgcg
    15241 tgcgattccg tgcaactcgc ctttgggccg tttgcgtaat tcgcggaccg cgcttaccgt
    15301 cttcgcgtgg aagcgctcca agacaaggtc agggtcctgc tggtcgacga cttcgcgctg
    15361 atgcgcaagg gcgtcaccgc tgcgctcgag agctcgggcg cgatgaccgt cgtcggcgag
    15421 gccgggaccg ttgaagaggc ggtggcgctc gcagagcggc tgcagcccga cgtcgtggtg
    15481 ctcgacatgc gtctccccgg cggcggcggc ctggtcgcga tcgaccgtct gcgcgagatc
    15541 gcgcccggcg cgcgcgtgtt ggtgctgacc gcgagcgaca agcccgagac gctgatcgag
    15601 gcggtctccg ccggcgccac cggctacctg accaagcgcg ccggcggcga ggagctctgc
    15661 gccgcggtcg cccaggtcgc gaccggcgcc tcggtcgtcg agccgtcgct cgccggccac
    15721 ctgatgcgtg agctcgctgg ggagcgcgac gtcggcggca gcgcgcgcgt gtcgctcacc
    15781 ggacgcgagc gtgacgtcat gcggctggtc tgcgagggcc tgaccgaccg cgagatcggc
    15841 gagcggctgt tcgtctcgac ccgcaccgtc cagaacgacc tgcgccgcct gcgcgacaag
    15901 gccgggctcg agcgccgctc cgagctcgtc cactgggcga cgagccggtc gctcgcatag
    15961 gtccgagatg aagatccggg tcctcgtcgc ggacgacttc ccgctcctgc tcgacggcct
    16021 ctgcacggcg ctcgacgccg accccgcgtt cgacgtcgtc gggcgagcgg ccgacggcgt
    16081 cgaggccgcc acgcgcgcgg ccgagctgag ccccgacatc gtgctgctcg acatgatgat
    16141 gccgcgctgc gacggccccg ccgcgataga gctggtccgc tcgcacgcgc ccggcgcgcg
    16201 cgtgatcgcg atctctgcga gcgagcggat cgagctggta cgcgacgcgc tcgacgccgg
    16261 cgcggccggc tacgtctcaa aggcctcgct cgcgcatgag ctgcgcgacg ccgtcatcca
    16321 cgtccacggc tcgcgcaacg gtccctatgt ctcgccgagc atcgccgacc gcgtcctgcg
    16381 ctcggagtcc gaccgccccg ggccggggga cgccggccgc tcgcgcgccg cgctgctcag
    16441 cccgcgcgag cacgacgtgc tgcgcctgat tgctcagggc tacagcgact cggcgatcgc
    16501 cgacgagctc agtatccgca tccgcaccgt tcagaaccac ctcgcacgga tcagggtcaa
    16561 gacgcgcctg cgccgccgcc ccgagctggt cacctgggcg atgcgccacg gcgtccgctg
    16621 acgctgcgcg gctgcgcagc gcagttgcgc gaatgcgcaa cgagcgcggc gtgaatcgct
    16681 ctactgaaca tcgccggtcg gccccacctt cggagtcagg cgcaccgaaa gcgccgaccg
    16741 gctcatgaca actcgacgac tcctcacaca gctcccggcc gccgctctgg cggccctcgc
    16801 gcttgccgcg cccgcggcga ccgccagctc gagcaccgag atcctcgttc cgagcggcag
    16861 cggcatcacc gctccctccg gcgccgcgct gacctcagac ggcgcgctct ggatcggcga
    16921 cggcgagaag ggcgtctgcc gcgttcggct cacccagccg cgcgggttgg tccgcgacgg
    16981 cgtctggtgc gaccccgaca cgccggccct cgacaagacc ggccagctcg ccttcgacag
    17041 cgagacgaac ttcctctacg tcgccgaggc cgactccgcg gccggcggta tctggcgcct
    17101 gcgctgggac cccggcacgg ggacgatcgt cagcggcggc aagatcatcg acacaccgga
    17161 cgacctgatc accggcctcg cggtgagcta caccggcccg ccgcgctacg agggtggccc
    17221 gctgaccggc gagctcaact tcacctccaa gcgcaccaac agcgtgcgcc gtgtgcgtga
    17281 gcccgacggt gcccagccga cggtcgtgac cgtcggccag cgccgcttct tcggcgagga
    17341 ggacatcagc tcgatcgccg ccctcggtcc ggacctctac ctcgccgagc cgttcggcgt
    17401 gacccgcctg accaccttcc ccggcgagac cccgcgcgcc gagctcgtcc ccggcctcgc
    17461 ggagcccttc cccgcgggcg tcgccagcgc ggtcgccgcc gatccgcagc gcggccgcat
    17521 ctacgccggc accgacaacg gcaacgcccg cgaccaggtc gacgtgctcg acgtcgccgg
    17581 cctgagccag gagctttacg acgtcggcgt cgccgccgtg atcgcgatcg tcgtcgagga
    17641 cgacggcgac ctgctgatcg tcgacgatcc ctccgacgct gcgggcgcgg acgtcaacaa
    17701 tcaagcgcgt gtgtggcgga tgcgccttgc gccgctcggc cggccacgcg ccacttggac
    17761 gcaggtcccg ccgctgttca cgtccgccac caacgccggc ttcggctaca ccgcccgcgc
    17821 cggctcgagc ttcgagtgct cgatcgacag cgcgccgtgg cagccctgcg gcgggccgac
    17881 gagcggctac tacccgatga gcgacctcgc cgagggcacc tacgtcttcc gcgtgcggcc
    17941 ggtcgacgcc gacccctcga tcggcgtcgg gcgtgcgatc acccgtccgc tggtcgtcga
    18001 ccggacgcgg cccacggtct cgatcgacaa caccgccgac gaccgcgacg tctacagcaa
    18061 cgagttcaag atccgcttca gcggcagcga gccgggcctc accttccgct gctcactcga
    18121 cggcgcgccg gccatcgcct gcacgcccgc gtaccactcg ttcgagggcc tgacccacgg
    18181 ctaccacacc gtcgaggtca cggcgaccga cgcggcgggc aacgattcgc tctccggccc
    18241 gcacgcccgc taccacttct acgtccacgc gccgtcgccg ccggtccggc cggcgccgct
    18301 gccgcccgtg ccgccggtcg tggctccgct gctgcccagc ccggcgcctg ccgcgaggac
    18361 cgccaagacg gtcgccccga agaagaagaa ggccgcgctc cgcccgaagg ccggctacgc
    18421 gcgcctcgcc ggccggcccc gtcgctgtgc ccgcggtggc ttcacggcct acgtcgccgg
    18481 gcgtgcgatt gaccgcgtcg gcttccgtct cgacggccgc cggatccggg tcgtgcgctc
    18541 cgccaaccgc cgcggccgct tcgcgatccg cgtcccggcc cgccgctccg gcccacacgt
    18601 cctgaccgcg ctgatccgct tcgaccgcgg cgccggccgc tccgagcggc tcgcgctcga
    18661 gtaccgcagc tgccccggag ggctccgatg actttctcct tcacgatcac ccgcgcccgg
    18721 ctcccgctgc tcgggttgat cgccgccgtg ctggcgctcg cgacgaccgg catcgtcctg
    18781 tccgtcgtca gcatcgccgg cggctcgtcg agcggccacg cccaccaggg gccggtgccc
    18841 aacggcccgc agaccgccgg tgagggcatt ccgaccagct tcggcgcggt cgccgtcgag
    18901 tacgcggtcc ggctcagcgg cttgacctcg aaggacctcg cgggcatgac ccacggcgtc
    18961 caggactacg tgccgccgga gagctcgcag gttcaggtcg cggtgacgct cgcgaacatg
    19021 ctcgacacga ccaacgagta cgcgccggag cagttccgcc tgattgccgg cacgagcgac
    19081 gtgcccggcc cgagcgccaa gcgcatcgtc ccgaccggcg ccaccttcaa gcccggcacg
    19141 ctgcagcccg acgccgcgat cgagggctcg ctgagcttcg tcgtgccgaa caagcgcgcc
    19201 cacctctggg tcgagttcca cgacccagag cgcgcggagc cgctgctcgt cgacctcggc
    19261 aagcccgccg cgacgctgcc cggtgcgccc ggtcgcgccg accgcaagcg gcccgagctg
    19321 cccgccccgc ccacgtcctt cgatggcacc acgccgcacg cccatccggg ccagtagcga
    19381 aagcgagtta ctccgatgac cgagacgatc ctccgcagca tcgcccgccg gcccgcgtcg
    19441 gcgctcgccc gcccgttcgc ggtcgccgtc tacgcgatcg tgctgtcgct cggcggcgtc
    19501 ctctggctga agctcgtcca cgcgctgcag ggctcgcacg agccgggcaa caccggcttc
    19561 ctgctcgagc tcggccgcga cttcgtgctg gcgctgccgc tgcagttcgc ctggatctgg
    19621 atcgccgtgc tgatgacccg ccgcgcgctg cagcgcagcg gcgagcagtc gccggcgctc
    19681 tcggcgaccg tcctcgccgt cgtcgtcgcg ctcgtcgcca gcctcgcgct cgcgctcggc
    19741 gcgccgctgc acgccgcgat cttcggcgcg atccccgcgc ccagccacga gatgccggcg
    19801 ctcgtccacc tcttctggga gaccgtcgtc ggcctcgtcg ccacgctgcc gctggccgcg
    19861 atcgcgaccg cgttcctgct gcgccgccgt ccctgggacg cgcccgacac gcgccgctgg
    19921 ctcgcgctcg cgccgtcggc gcagcgcgtc cggctcgcgt cgctgctgct cgtgctcgtg
    19981 ctcgccccgg ccgcgacctt cgccggcagc ggcgtcgatg gcgcgaccgc cgacctctcg
    20041 ccgggcagcc cgtgcccggc cggtgcgccg accaagacct acgacgtgcg cgcgatcgac
    20101 gtcgacgtgc cgctgaaccg cttcggcgac cacgacccca acggcaagat gttcgtgctc
    20161 gccgaccgcg tcgacgacgt gcgcgagcag gaggcctccc agcacgtctc gatcggcctg
    20221 cgcgacgacg cgatccagcc gctggtgatc cgcgcgaacc tcggcgactg cgtcgagatc
    20281 gagttcgcca acgaggcctc gggcggcgag tacggcgtcc acatcgacgg cctctccttc
    20341 gacatgggct cgtccggcga cgcggtcggc aacaacgcgc cgtccgcggc cgcgcgcggc
    20401 gagtcgcgca cctaccgcta ctacgtcccc gaggagcccg agctcgaggg cgcccactac
    20461 atgcgccccg gccccggcaa ccgccaggcg gtcgcccacg gcctgttcgg cacgttgctc
    20521 gtcgagccgc ccggatccga gtacctcgac atggacaccg gcgagccgat cgactccggc
    20581 tgggaggcga tgatcgtccc cggcgacggc aagacggcct tccgcgagta cgcgatcatc
    20641 cagcacgaga tcggcgacga gagcgccgat gtgttcgacc gcgccggcga gcggctgccc
    20701 gtcgtcgacc cgctgaccga cggctaccgc cccggctccc gcgcgatgaa ctaccgctcg
    20761 gagccgttca tgcgccggct cgagcgcaac gggcggatga agtcccacgc ctacggctcc
    20821 tacgccttcg gcgaccccgc gacgccgatg ccgcgcagct accagggcga cccgaccaag
    20881 atgcggatcc tccacggcgg ctcggagacc ttccacgtct accacctgca cggcggcgcg
    20941 atccgc
//
LOCUS       DATABV010000180        11481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2546202, whole genome shotgun sequence.
ACCESSION   DATABV010000180 DATABV010000000
VERSION     DATABV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11481
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2546202"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1214)
                     /locus_tag="VHF88_07985"
     CDS             complement(45..1214)
                     /locus_tag="VHF88_07985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX2161746.1"
                     /translation="MRNGSREVVIVEAVRTPIGRGHKEKGYYKDTHPSNLLSKTYSEV
                     VERSGVDASEVEDVIAGCVQQFGEQAYNIGRNAWLEAGLPIETPATTIDRQCGSAQQA
                     VNFAAAQIGAGVLDCAIGSGVEHMGHIGFGDGMKVIGEYGSPFTPELMEKYNLIPQGL
                     SAEMIADQWEIPRSELDELAVRSHRLANQATEEGRFEREIVPFAVNGDTHVTDQGIRP
                     GTDIETLAGLKPAFKEDGKITAANSSQISDGAAAVLLMERGKAESLGLKPRARIVDQI
                     TVGVDPVIMLTGPIPATQKLLERNGMQIGDIDLFEVNEAFAPVVAAWQRELEPDMDRV
                     NVNGGAMALGHPLGSTGARLITTLLHELERSDKSVGLVTMCCGGGLGTGTLIERV"
     gene            1374..2156
                     /locus_tag="VHF88_07990"
     CDS             1374..2156
                     /locus_tag="VHF88_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161747.1"
                     /translation="MGGDAGSERLGIAGSGAIACGLARTAEQRLPAVPTLLWARSDDS
                     AARAAERLGAHGEVTTDLERLAGCTVVVEAVAEDAAVKADLLARLGELLPPDALLAST
                     TSSLSVAELAQASGRPDRFGALHVFNPVEKMKLVELAFAPDAADATRARLRGLCDALG
                     KTAVEVADAPGFVVNRLLFPYLFDAVRLMEAQGLEPQAVDACMKLGAGHPMGPLALLD
                     FVGLDVAVAIGESIDADVPDRIRQMASDGRLGRKSGSGFYDY"
     gene            complement(2179..3528)
                     /locus_tag="VHF88_07995"
     CDS             complement(2179..3528)
                     /locus_tag="VHF88_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2161748.1"
                     /translation="MTELLLLVGVAVLVAANGFFVAAEFALVRARDNKVEQMVEDGLR
                     GAALVRTQIDRIDEYLSACQLGITMASIGIGFLGEPAIASLLEEPLGGVMSHGVAVAI
                     AFTVSYLIVTSAHITVGEQVPKIYAITHPEGTARRIAGPLAAFRKLFKPLIWALNSTS
                     NAMLRVIGVDAKADFDDDLSNAEDLKLLIVRSATGGKIDPGEAGMLSGVFHLHEQEAR
                     QVMTPIPAVVTVDVSETAEEALRRCVDSGHTRLVVTEDGNTDRVRGIVHNNSLARRVL
                     NEGPDASIESSVKDALIVPETKSLDDLLADLQRERTSLAVVIDEYGRTSGIVSIEDIV
                     EEVVGEIADETDPAVAGIRQLANGDWWVRGHVPITDLADYGLELPIDSDAYNSVGGFV
                     FGELGRLPKRGDMVRANGYSLRVESVRENRIDAVRIRDHGEQPAPVEAEPRESAEGA"
     gene            complement(3628..3909)
                     /locus_tag="VHF88_08000"
     CDS             complement(3628..3909)
                     /locus_tag="VHF88_08000"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX2161749.1"
                     /translation="MADLLERDEVAQRVEELDGWDLEGEAIVKQFKRGDFSGAVGLLN
                     AIEPVANELNHHPDVSISWETVTVMITTHSAGGLTAADFELAARIDALA"
     gene            complement(3953..4795)
                     /locus_tag="VHF88_08005"
     CDS             complement(3953..4795)
                     /locus_tag="VHF88_08005"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2161750.1"
                     /translation="MADCYRHPGRETGVSCSNCGRPICPDCMTATPVGMRCPECARDR
                     TKVRRAASVGVGEDPMLTYVLIGINVAVALGAFLSGASATGGGGIGSSSLLADGAVSR
                     FHVDQGDYWRLLTSGFLHTGLIHLLFNMFALYILGGMLEPAIGRLRFAIVYFVSLFAG
                     SFGALLLTPDGATAGASGAIFGLMGAAVIVMRDRGINPMESGIGLWIGLNLLITFTIP
                     GISIGGHIGGLIGGALAALVFVELAGRMRASDRLTSLIVGAIGVIAIAGAIAISSGAA
                     AGFA"
     gene            complement(4862..5383)
                     /locus_tag="VHF88_08010"
     CDS             complement(4862..5383)
                     /locus_tag="VHF88_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161751.1"
                     /translation="MGLRRSINGGLAGGIAAALWAAQQPLDKRAFGSAYDDVELVGKA
                     IAKGDDWPAAGLAIHVLNGVAFGAAYAQLRRFLPGPAIAQGTGMAVAEHFGLWPLVTV
                     VDRYHPRRGELTALKGNRAAFRQGLYRHILFGLVMSAIEAKLNADPVEDEPAPVPVSS
                     NGHGDIKRAVTAA"
     gene            5428..6243
                     /locus_tag="VHF88_08015"
     CDS             5428..6243
                     /locus_tag="VHF88_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2161752.1"
                     /translation="MPATGPTRPRRDGELRVSAPPAAPRPGEDEHLSPHHRRARERGV
                     NRLVYWTVRAFLQPAMHIYFRLRRTGREHIPKGQVILAANHRSFLDPFVVGACTFRPV
                     YFVAKKELFDKRWQGWLLNCLGAFPIKRGQSDEESMQTALALLARGEAVVIFPEGTRH
                     REGPLQEPRRGVGRLALESGAPVVPIAITGTEKARRGWRIRPVKVSIRCGRPLRYPQV
                     TDPSPELAGEVTARIWPCVELQWAWLGGPLPARGPDPPPDPAQRPRSSERSVA"
     gene            6280..6834
                     /locus_tag="VHF88_08020"
     CDS             6280..6834
                     /locus_tag="VHF88_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2161753.1"
                     /translation="MGKAADKRRLDADFTELYRSHLRDVYSYAYYRVGDHHDAEDLTE
                     QTFLQAYRHFERAQRESDGRPLRPWLIRIAHNLAANYYRDRSRKPQTHLEDAAVLSAP
                     HGTEELVEGRQELQHVLEGVATLPDDRREALIMRFALGMDNREIARALDRSEGATKVL
                     IHRAIKQLEKGLEERAGKVVGDER"
     gene            6824..7159
                     /locus_tag="VHF88_08025"
     CDS             6824..7159
                     /locus_tag="VHF88_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161754.1"
                     /translation="MNAESTDVEALLRSALVPVEPSERMTDRLERSLSDITDIAAGEL
                     ADWELSAMRDPRNWGRPAAALVVGGVAAGGLVLVRARQARRRDGASVRALERSLRQVA
                     SEIQTRLDR"
     gene            complement(7186..7258)
                     /locus_tag="VHF88_08030"
     tRNA            complement(7186..7258)
                     /locus_tag="VHF88_08030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7223..7225),aa:Thr,seq:tgt)
     gene            complement(7249..7701)
                     /locus_tag="VHF88_08035"
     CDS             complement(7249..7701)
                     /locus_tag="VHF88_08035"
                     /inference="COORDINATES: protein motif:HMM:NF023075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HEX2161755.1"
                     /translation="MDGGEIIRRLRTRVPSRHTVDVGLQTEAVITSELLRRGIRVLLP
                     CGFNHRYDLVLDLDGDFVRVQCKTGRLRRGVVHFNSESVRSNSKAALRRAYDGEAEIF
                     VVYCQETDEIYAVPVADAPKRGVTLRVEPTANSQAEGVRWARDYILPA"
     gene            complement(7788..7952)
                     /locus_tag="VHF88_08040"
     CDS             complement(7788..7952)
                     /locus_tag="VHF88_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161756.1"
                     /translation="MITLLVAALAYLLLVAITGSAIVGIVAAVLILIAGIPSGGFGIG
                     GRWGARGAPR"
     gene            complement(8044..8880)
                     /locus_tag="VHF88_08045"
     CDS             complement(8044..8880)
                     /locus_tag="VHF88_08045"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX2161757.1"
                     /translation="MRRSTRLLAIALPVALATVPVALAGADSAAGDRDSGAVPVAHDP
                     LVDPEAATSIAAAASTKAAASVAAEALAKATRPPPAAIVAVAPGRTVVLRVRPGGPVA
                     LRVGDTTEFGSRQRLGVVRRAGRWLGVTTPELPNGTLGWLERRSPAVEVRRTNWSIVA
                     DVSRRTVVLRRAGKPVRRLSVAVGRPGSPTPTGRFAVTDKLSGGDYGSYYGCCILALS
                     ATQPNTPPGWTGGDRMAIHGTSDPGSIGAAASAGCLRAADGDLEYLMGRAPLGTPVVI
                     RR"
     gene            complement(8946..9611)
                     /gene="sigH"
                     /locus_tag="VHF88_08050"
     CDS             complement(8946..9611)
                     /gene="sigH"
                     /locus_tag="VHF88_08050"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX2161758.1"
                     /translation="MAATISQSPLARSQAQLELEDNYLIALAKQGRPDAYDKIVRRYY
                     GFVRLKASSYFLIGGDADDLIQEGLVGLYKAVRDFRTDRESSFRNFAELCITRQIITA
                     VKTSTRNKHTPLNEYVSFSQTPAASSGDGDPTLDEMLPGPTVHDPVNQVISSEELHSL
                     VGCLSSVLSELESSVLSLYLDGHSYEAVSERLDCDTKTVDNALQRVKRKVGTHLRSRE
                     VQL"
     gene            complement(9742..10455)
                     /gene="rlmB"
                     /locus_tag="VHF88_08055"
     CDS             complement(9742..10455)
                     /gene="rlmB"
                     /locus_tag="VHF88_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX2161759.1"
                     /translation="MIVYGRNPVREALRGPRAVERIWATQRALDGEEWLRGSDTRVVD
                     GAEVERLCGSAEHQGVCAEVSAYRYVDAHELLAAEDALVVCLDEVQDPHNLGAVCRVA
                     ESAGASGVVIPERRSAEVTAAAAKASAGAVEHLPVARVGNLANWLGDAKQAGAWVYGA
                     EAGSEAAHYATLDYRGRVVLVLGSEGRGLRPRVADSCDQLVALPQRGRLNSLNVSTAA
                     AALLYGILHLREAGLDKAP"
     gene            complement(10452..>11481)
                     /locus_tag="VHF88_08060"
     CDS             complement(10452..>11481)
                     /locus_tag="VHF88_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX2161760.1"
                     /translation="RAVDTIGPIVDLIQALIDGGHAYAAGGDVYFDVSSFADYGKLSN
                     RPREEREQGEGDDAAALKRNREDFALWKAHKEGEDTCWPAPWGDGRPGWHIECSAMAE
                     QLLGTDFEIHGGGSDLVFPHHENEIAQTEAARGEPLARIWMHNGMVRMGDEKMAKSVG
                     NIRLLHGALDEHGRDALVMYFVGGHYRQPIAFSAEALAEAGRAVERIRELCRRLDPDA
                     PAPQDLDGYAERFFDALADDFNTPAARAVLFEWVAEANRRLDSGERIGAGRLEEMLHA
                     LGLESLLAAGDEDVEVDPEAARLLAERQDARASRDFERADAIRDRLAALGWTVRDTGD
                     GARLVRST"
BASE COUNT         1720 a         4039 c         4020 g         1702 t
ORIGIN      
        1 ggccggcggc gctcagccgc cggcctcatc aacttcgacg ccgtctagac ccgctcgatc
       61 agcgtccccg tccccaggcc gccgccgcag cacatcgtca ccagcccgac gctcttgtcc
      121 gagcgctcca gctcgtgcag cagcgtcgtg atcagccgcg cgccggtcga gccgagcgga
      181 tgaccgagcg ccatcgcgcc gccgttgacg ttgacgcggt ccatgtcggg ctcgagctcg
      241 cgctgccagg ccgcgacgac cggggcgaac gcctcgttga cctcaaacag gtcgatgtcg
      301 ccgatctgca tcccgttgcg ctcgagcagc ttctgcgtcg cggggatcgg accggtcagc
      361 atgatgaccg ggtcgacgcc gacggtgatt tggtcgacga tccgggcgcg cggcttcagc
      421 ccgagcgact ccgccttgcc gcgctccatc agcaggaccg cggccgcgcc gtcggagatc
      481 tgcgaggagt tggcggcggt gatcttgccg tcctccttga aggccggctt cagcccggcg
      541 agcgtctcga tgtcggtgcc gggacggatg ccctggtcgg tgacgtgggt gtcgccgttg
      601 accgcgaacg ggacgatctc gcgctcgaag cgcccctcct cggtcgcctg gttggcgagc
      661 cggtgcgagc ggacggcgag ctcgtcgagc tcggagcgcg ggatctccca ctggtcggcg
      721 atcatctcgg ccgacagtcc ctgcgggatc aggttgtact tctccatcag ctcgggcgtg
      781 aacggcgagc cgtactcacc gatcaccttc atgccgtcgc cgaagccgat gtgacccatg
      841 tgctcgacgc ccgagccgat cgcgcagtcg agcaccccgg cgccgatctg cgcggcggcg
      901 aagttgaccg cctgctgcgc cgagccgcac tggcggtcga tcgtcgtggc gggggtctcg
      961 atcggcaggc cggcctcgag ccaggcgttg cggccgatgt tgtaggcctg ctcgccgaac
     1021 tgctggacgc agccggcgat cacgtcctcg acctcggacg cgtcgacgcc ggagcgctcg
     1081 acgacctcgc tgtaggtctt cgagagcagg ttgctcgggt gggtgtcctt gtagtagccc
     1141 ttctccttgt gcccgcgccc gatcggcgtg cgcaccgcct cgacgatgac gacctcgcgg
     1201 cttccgtttc gcatctcgct gagtcccttc ggcaggtttc gatgcggcgc ggcgccctct
     1261 ctgcgcgcgc ctaccgagtc aaggttactc gattgactgg cgagtcaacc aggatcgggg
     1321 acggtctcga tcggccctag acagagcggt tttcgactat tctcgcgcgc ctgatgggcg
     1381 gcgacgcggg cagcgagcgg cttggtatcg ccggcagcgg cgcgattgcc tgcgggttgg
     1441 cgcgcaccgc cgagcagcgc ctcccggcgg tgccgacgct gctctgggca cgcagcgacg
     1501 actccgccgc ccgtgccgcc gagcgcctcg gcgcacacgg cgaggtgact acggacctcg
     1561 agcggctcgc cggctgcacg gtcgtcgttg aggccgttgc cgaggacgcg gcggtcaagg
     1621 cggacctgct cgcccggctc ggcgagctgc tcccgcccga cgcgctgctg gcctcgacca
     1681 cctcgtcgct gtcggtcgcc gagctcgcgc aggccagcgg ccgcccggac cgcttcggag
     1741 cactccacgt cttcaacccc gtcgagaaga tgaagctcgt ggagctggca ttcgcgccgg
     1801 acgctgccga cgcgacgcgc gcgcgcctgc gcggcctctg cgacgcgctc ggcaagacgg
     1861 ctgtcgaggt cgccgacgcg ccgggcttcg tcgtcaaccg cctgctgttc ccctacctct
     1921 tcgacgccgt ccgcctgatg gaagcccagg gactcgagcc gcaggcggtc gacgcctgca
     1981 tgaagctcgg cgccggccac ccgatgggtc cactcgcgct gctcgacttc gtcggcctcg
     2041 acgtcgcagt ggcgatcggc gaatcgatcg acgccgatgt gccggatcga atccgccaga
     2101 tggcctctga cggccgtctc ggacgcaagt ccggtagcgg cttctacgac tactagccgc
     2161 gctcgccgcg cccgtcggct acgcgccctc ggccgactcg cgtggctccg cctcgaccgg
     2221 cgccggctgc tcgccgtggt cgcggatccg caccgcgtcg atgcggttct cgcgcacgga
     2281 ctcgacgcgc agcgagtagc cgttggcgcg caccatgtcg ccgcgcttgg gcagccgccc
     2341 gagctcgccg aagacgaagc cgccgaccga gttgtaggcg tcggagtcga tcggcaactc
     2401 gagcccgtag tcggcgaggt cggtgatcgg cacgtggccg cggacccacc agtcgccgtt
     2461 ggcgagctgg cggatgccgg cgacggcggg gtcggtctcg tcggcgatct cgccgacgac
     2521 ctcctcgacg atgtcctcga tcgagacgat gccgctcgtg cggccgtact cgtcgatcac
     2581 caccgccagc gacgtccgct cgcgctggag gtcggcgagc aggtcgtcga gcgacttcgt
     2641 ctcggggacg atcagcgcgt ccttgacgct cgactcgatc gaggcgtcgg gcccctcgtt
     2701 gaggaccctg cgagcgagcg agttgttgtg gacgatgccg cgcacgcggt cggtattgcc
     2761 gtcctcggtg acgaccagcc gcgtgtggcc ggagtcgacg caacggcgca gcgcctcctc
     2821 ggcggtctcc gagacgtcga cggtgacgac cgccggtatc ggcgtcatca cctggcgcgc
     2881 ctcctgctcg tggaggtgga agacgccgga gagcatcccc gcctcgccag ggtcgatctt
     2941 gccaccggtg gcgctgcgga cgatcagcag cttgaggtcc tcggcgttgg agaggtcgtc
     3001 gtcgaagtcc gccttggcgt cgacgccgat cacgcgcaac atcgcgttcg aagtgctgtt
     3061 gagcgcccag atcagcggct tgaagagctt gcggaaggcg gccagcggtc ccgcgatgcg
     3121 gcgcgccgtg ccctccggat gggtgatcgc gtagatcttc ggcacctgct cgccgactgt
     3181 gatgtgggcg ctggtcacga tcaggtagga gacggtgaag gcgatcgcga ccgcgacgcc
     3241 gtggctcatc acaccgccga gcggctcctc gagcaggctc gcgatcgccg gctcgccgag
     3301 gaagccgatc ccgatcgacg ccatcgtgat gcccagttgg catgcggata ggtactcgtc
     3361 gatgcgatcg atttgcgttc gcacgagcgc cgcgccgcgc aggccgtcct cgaccatctg
     3421 ctcgaccttg ttgtcgcgcg cacggacgag cgcgaactcc gcggcgacga agaagccgtt
     3481 ggccgcgacg agcacggcca cgccgaccag gagcagcagc tcggtcacgg cgggccctcg
     3541 cgggctggca ggcccgtctg ggcggaccta cttcgaggag gctcgtcgtt catccgatga
     3601 ggccgcaacg tagcagccga agccgcgcta ggcgagtgcg tcgatgcggg cggcgagctc
     3661 gaagtcggcg gctgtcagcc cccccgcgga gtgggtcgtg atcatcacgg tcacggtctc
     3721 ccaggagatc gagacgtcgg gatggtggtt cagctcgttc gccaccggct cgatcgcgtt
     3781 gaggaggccg acggcgccgg agaagtcgcc gcgcttgaac tgcttgacga tcgcctcgcc
     3841 ctcgaggtcc cagccgtcga gctcctcgac ccgttgggcg acctcgtcgc gctccagaag
     3901 atcagccatg gacgcagtat ccccagcggc tggcggaagc gcacatggcg gcctacgcga
     3961 agccggcagc ggcgcccgag gagatcgcga tcgcgccagc gatcgcgatc acgccgatcg
     4021 cgccgacgat cagcgacgtc aggcggtccg acgcacgcat ccgcccagcg agctcgacga
     4081 agacgagcgc cgcgagcgca cccccgatca gcccaccgat gtggccgccg atcgagatac
     4141 cgggaatcgt gaacgtgatc agcaggttga gcccgatcca gagaccgatg ccgctctcca
     4201 tcgggttgat cccgcggtcg cgcatcacga tcaccgccgc gcccatcaga ccgaagatcg
     4261 cgccagaggc gccggccgtg gccccgtcgg gagtcagcag cagcgctccg aacgagccgg
     4321 cgaacagcga gacgaagtag acgatcgcaa agcgcagccg cccgatcgcc ggctcgagca
     4381 tcccgcccag gatgtagagc gcgaacatgt tgaacagcag gtggatcagg ccggtgtgga
     4441 ggaagcccga ggtcagcagc cgccagtagt cgccctggtc gacgtggaag cgcgacaccg
     4501 ccccgtcggc gagcagcgac gagctgccga tgccgccgcc cccggtcgcg ctcgcgccgc
     4561 tcaggaacgc gccgagcgct actgcgacgt tgattccgat cagcacgtag gtcagcatcg
     4621 ggtcctcgcc gacgccgacc gaagccgcgc ggcgcacctt ggtgcgatcg cgcgcgcact
     4681 cggggcagcg cataccgacc ggcgtcgcgg tcatgcagtc ggggcagatc ggccggccgc
     4741 agttcgagca ggagaccccc gtctcgcgcc caggatggcg atagcagtcg gccacgctgg
     4801 cagcctagtg acccagccag gcgcagcggg ggcacggggg cgcgagcccc cgacctacct
     4861 cctatgcggc ggtgacggcg cgcttgatgt cgccgtggcc gttcgacgag accggcacgg
     4921 gcgccggctc gtcctcgacg gggtcggcgt tgagcttcgc ctcgatcgcg ctcatcacga
     4981 gcccgaacag gatgtgccgg tagaggccct gccggaacgc ggcccggttg cccttgagtg
     5041 cggtcagctc gccgcggcgc gggtggtagc ggtctacgac cgtgaccagc ggccagagcc
     5101 cgaagtgctc ggcaaccgcc atgccggtgc cctgtgcgat cgccggcccg ggcaggaagc
     5161 gccgcagctg cgcgtaggca gctccgaacg cgacgccgtt gaggacgtgg atcgcgaggc
     5221 cggcggccgg ccagtcgtcg cccttggcga tcgccttgcc gaccagctcg acgtcgtcgt
     5281 acgcgctgcc gaaagcccgc ttgtccagcg gttgctgcgc cgcccagagc gcggcggcaa
     5341 tgccgccggc cagcccaccg ttgatgctgc gacgcaagcc cattccgcat gagcgtaacg
     5401 gcgacgcctg gcacgggtag ctgaggtatg ccggcgaccg gtcccacgcg ccccaggcgc
     5461 gacggcgagc tgcgcgtctc ggcgccgccc gcggccccgc ggccgggcga ggacgagcat
     5521 ctctcgccac accatcgccg cgcgcgcgag cgcggcgtca accgactggt ctactggacg
     5581 gtgcgcgcgt tcctgcagcc tgcgatgcac atctacttcc gcctgcgtcg caccggccgc
     5641 gagcacatcc cgaagggcca ggtgatcctc gcggcgaacc accgcagctt cctcgacccg
     5701 ttcgtcgtcg gcgcctgcac gttccggccg gtgtacttcg tcgccaagaa ggagctgttc
     5761 gacaagcgct ggcagggctg gctgctcaac tgcctcggcg catttccgat caagcgcggg
     5821 cagtcggacg aggagtcgat gcagacggcg ctggcgctgc tcgcgcgcgg cgaggcggtc
     5881 gtgatcttcc ccgagggcac ccgtcaccgc gaggggccgt tgcaggagcc gcggcgcggc
     5941 gtcggtcggc tggcactcga gagcggcgcc ccggtggtgc cgatcgctat caccgggacc
     6001 gagaaggcac gccgcggctg gcggatccgg ccggtcaagg tgagcatccg ctgcggtcgg
     6061 ccgctgcgct acccgcaggt caccgacccg tcgccggagc tggcgggcga ggtgaccgca
     6121 cgcatctggc cctgcgtcga gctgcagtgg gcgtggttgg gcggcccgct gccggcgcgc
     6181 gggccggacc caccgccgga cccggcgcaa cggccgcgca gctccgagcg gagcgtcgcc
     6241 tgatggaggt caagatcgcc gcagtaggct gcacgccacg tgggcaaagc cgccgacaag
     6301 cgccggctag acgccgactt caccgagctg taccgctccc atctgcgcga cgtctacagc
     6361 tacgcctact accgggtcgg cgaccaccac gacgccgagg acctgaccga gcagacgttc
     6421 ctgcaggcct accggcactt cgagcgtgca cagcgcgagt ccgacggccg gccgctgagg
     6481 ccttggctga tccggatcgc gcacaacctc gcggcgaact actaccgcga ccgctcgcgc
     6541 aagccgcaga cacacctgga ggacgcggcg gtgctctccg caccacacgg cacagaggag
     6601 ctggtcgagg gccggcagga gctccaacac gtgctcgagg gcgtcgccac gctgcccgac
     6661 gaccggcgcg aggcgctgat catgcgcttc gcgctcggca tggacaaccg cgagatcgcg
     6721 cgggcgctcg accgcagcga gggcgcgacc aaggtgttga tccatcgtgc gatcaagcag
     6781 ctggagaagg ggctcgagga acgtgcaggg aaggtcgtag gcgatgaacg ctgagtccac
     6841 cgatgtcgag gcgctgctgc gctctgcgct cgtgccggtc gagccatccg aacggatgac
     6901 cgaccgcctg gagcgcagcc tctccgacat caccgacatc gccgccggcg agctcgccga
     6961 ctgggagctc agcgcgatgc gcgacccacg caactggggc cgtccggcgg ccgccctggt
     7021 cgtcggcggc gtcgccgccg gcggactcgt gctcgtccgc gcccgtcaag cacgccggcg
     7081 cgacggcgct agcgtgcgcg cgctggagcg cagcctgcgc caggtcgcca gcgagatcca
     7141 aacccgcctg gatcgctgac tcacgcgcag attctctctc cgtcaagccc gccctcggac
     7201 tcgaaccgag aacctctcca ttacaagtgg agtgctctac caattgagct aggcgggcaa
     7261 gatgtagtca cgcgcccagc gaacgccttc ggcctgtgaa ttcgccgttg gctctactcg
     7321 aagcgtgacg cccctcttcg gtgcgtctgc gacgggcaca gcatagatct cgtccgtctc
     7381 ttggcagtag acgacgaaga tttccgcctc gccatcgtag gctcgccgca acgccgcctt
     7441 cgagttggaa cgaacgctct cggagttgaa atggacgacg ccacgtcgga gccttccggt
     7501 cttgcactga accctgacga agtcgccgtc caagtccaag acgaggtcgt agcggtgatt
     7561 gaagccgcat gggaggagca cccgaatacc gcgacgcaga agctccgacg tgatcaccgc
     7621 ttccgtctga aggcccacat ccacggtatg ccggctcggt actcgagtcc ggagtcgccg
     7681 gatgatctcg ccgccatcca cgacatggac tttatgccat gccgcagaca gcagcaaagc
     7741 gggcgccccg aggggcgccc gccttggaaa gagatgcgtc ctgcggttta gcgcggggcg
     7801 ccgcgggcgc cccagcggcc gccgatgccg aaaccgccgg atgggatgcc ggcgatcagg
     7861 atcaggacgg cggcgacgat gccgacgatc gcggatccgg tgatggccac cagcaacaga
     7921 taggccagtg ctgctacgag cagtgtgatc aagagtccga ccatttcggt ttcctccatc
     7981 ttcgggggtt gcagatgtag tacccggggc gagagaacgg ctaaacggtc gctaccgctt
     8041 gcgctaccgc cgaatgacga caggcgtgcc gagcggcgcc cgacccatca ggtactcgag
     8101 gtcgccatcc gccgcgcgaa ggcatccggc cgaggcggcc gcgccgatcg agcccgggtc
     8161 gctcgttccg tggatcgcca tccggtcgcc gcccgtccag ccgggcggcg tattgggctg
     8221 agtcgcggac agcgcgagga tgcagcagcc gtagtaggag ccgtagtccc caccggagag
     8281 cttgtccgtg accgcgaagc ggccggtggg cgtcggcgag ccgggccgac cgacggccac
     8341 gctcaaccgc cgcaccggct tgccggcgcg acgcaggacg acggtccggc gcgagacgtc
     8401 cgcgacgatc gaccagttgg tgcgcctcac ctcgacagcg ggtgagcggc gctcgagcca
     8461 tcccagcgtc ccgttcggga gctccggcgt cgtgacaccg agccagcgac cggcacggcg
     8521 aacgacgccg agacgctgcc tcgagccgaa ctcggtggtg tcgccgacgc gaagggcgac
     8581 gggtccgccg gggcggacgc gcaggacgac ggtgcgaccc ggtgctacgg cgacgatcgc
     8641 tgcgggcggc ggcctcgtgg ccttggcgag cgcctcggcc gcgacggacg ctgccgcctt
     8701 ggtcgaggca gcggccgcga tggaagtggc ggcttcggga tcgacgagcg gatcatgagc
     8761 gactggcact gccccggagt cccggtcgcc ggcggccgag tcggcccccg cgagcgctac
     8821 ggggaccgtg gcgagggcga cgggtagggc gatggcgagc aggcgggtcg agcggcgcac
     8881 atcgcgtgca tcggctcgac ggcccgatca ctcaaggccc ggaacgctgg gcccactgcg
     8941 gccgactaga gctgcacctc gcgcgagcgc aggtgcgtgc cgaccttgcg cttgacgcgc
     9001 tgcagcgcgt tgtcgaccgt cttggtgtcg cagtcgagcc gctcggagac cgcctcgtag
     9061 ctgtggccgt cgaggtagag cgacaggacc gagctctcga gctcggacag cacgctcgac
     9121 aggcagccga cgaggctgtg cagctcctcg ctggagatca cctggttgac cgggtcgtgg
     9181 acggtcggtc ccggcagcat ctcgtcgagc gtcggatcgc cgtcgccgct ggaggccgcc
     9241 ggcgtctgtg agaacgagac gtactcgttg agcggcgtgt gcttgttgcg cgtcgaggtc
     9301 ttgacggcgg tgatgatctg gcgggtgatg cagagctccg cgaagttgcg gaagctcgac
     9361 tcgcggtcgg tgcggaagtc gcgaacggcc ttgtagaggc cgaccagtcc ctcctgaatc
     9421 aggtcgtcgg cgtcaccgcc gatcaggaag taggagctcg ccttgagccg cacgaagccg
     9481 tagtaacgcc ggacgatctt gtcgtaggcg tcgggacggc cttgcttggc gagggcgatc
     9541 aggtagttgt cctccagctc gagttgagcc tgggatcggg ccaacgggga ctgggaaatc
     9601 gttgcagcca tgcatgctcc ttccggaccg gaatcggccc ttgaagctca tggcgcgcta
     9661 cctcccctgg cgccgcgctc atcagcgaag gtcaagcctt aagctcaact ccacccttat
     9721 ctgagcggca gtattcagag gttatggagc cttgtcaagc ccggcttcgc gcaaatgcaa
     9781 gattccgtac agcagcgccg ccgccgccgt ggagacgttc aacgagttca accgaccgcg
     9841 ctgaggcagc gcgacgagct ggtcgcagct gtcggcgacg cgtgggcgca gaccccgccc
     9901 ctccgatccg aggaccagca cgaccctgcc gcggtagtca agcgtggcgt agtgggcggc
     9961 ctcggagccg gcctcggcgc cgtagaccca cgcgcccgcc tgcttcgcat cgccgagcca
    10021 gttggcgagg ttgccgacgc gtgcgaccgg cagatgctcg accgcgcccg cggaggcctt
    10081 ggcggcggcg gcggtgacct ccgccgagcg gcgctccggg atcacgacgc cgctggcgcc
    10141 ggcgctctcg gccaccctgc agacggcgcc gaggttgtgc gggtcctgga cctcgtcgag
    10201 gcagacgacc agcgcgtcct cggctgcgag cagctcgtgg gcgtcgacat agcggtaggc
    10261 cgagacctcg gcgcagacgc cctggtgctc ggcggagccg cacagccgct cgacctccgc
    10321 gccgtcgacg acgcgcgtgt cgctgccgcg caaccactcc tcgccgtcta gggcgcgctg
    10381 cgtcgcccag atgcgctcga ccgcgcgcgg tccgcgcagc gcctcgcgca cggggttgcg
    10441 cccgtagacg atcacgtgct gcgcacgagc cgcgcgccgt cgccggtgtc acggacggtc
    10501 cagccgagcg cggccagccg gtcacgaatc gcgtcggcgc gctcgaagtc tcgcgacgcg
    10561 cgcgcatcct ggcgctccgc gagcagtcgc gcggcctcgg ggtcgacctc gacatcctcg
    10621 tcgccggccg cgagcaggct ctcgaggccg agcgcgtgga gcatctcctc gagtcgtccg
    10681 gcgccgatcc gctctcccga gtcgagccgc cggttcgcct ccgcgaccca ctcgaacagg
    10741 accgcccggg cggccggcgt gttgaagtcg tcggcgagcg cgtcgaagaa acgctcggca
    10801 tagccgtcga ggtcctgcgg ggccggcgcg tcggggtcga ggcgacgaca cagctcgcgt
    10861 atgcgctcga ccgcgcgacc ggcctccgcc agtgcctccg ccgagaaggc gatcggctgg
    10921 cgatagtgcc cgccgacgaa gtacatgacc agcgcgtcgc ggccgtgctc gtcgagcgcc
    10981 ccgtgcagca gccggatgtt gccgaccgac ttggccatct tctcgtcgcc catccgcacc
    11041 atgccgttgt gcatccagat ccgcgcgagc ggctcgccgc gcgccgcctc ggtctgcgcg
    11101 atctcgttct cgtgatgggg gaagacgagg tccgagccac cgccgtggat ctcgaagtcc
    11161 gtgccgagca gctgctcggc catcgccgag cactcgatgt gccagccggg acgcccgtcg
    11221 ccccagggcg cgggccagca ggtgtcctcg ccctccttgt gagccttcca aagggcgaag
    11281 tcctcgcggt tgcgcttcag cgccgcggcg tcgtcgccct cgccctgctc gcgctcctcg
    11341 cgcggacggt tcgagagctt gccgtagtcg gcgaagctcg agacgtcgaa gtagacgtcg
    11401 ccgcccgcgg cataggcgtg gccgccgtcg atcagcgcct ggatcaggtc gacgatcggg
    11461 ccgatcgtgt cgaccgcgcg c
//
LOCUS       DATABV010000181        13191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_699688, whole genome shotgun sequence.
ACCESSION   DATABV010000181 DATABV010000000
VERSION     DATABV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699688"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VHF88_08065"
     CDS             <1..500
                     /locus_tag="VHF88_08065"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX2161761.1"
                     /translation="EATALPILRKDFTVDPYMLYEARAAGADAVLLVVGSIDEAELAE
                     LLAASRTLDLDAIVEVHEPDELELALAVDADVIGINNRDLADFSVDVARTFDLLPDVP
                     AGKVVVSESGIVRREQIEELERVGVDAVLVGETVMRADDPEAAVRALVGHEHSVASDG
                     PSGGV"
     gene            535..1776
                     /locus_tag="VHF88_08070"
     CDS             535..1776
                     /locus_tag="VHF88_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2161762.1"
                     /translation="MNETTPPQKRSSISTFLAGALGGLIVLVLGAALIATDVIDTGDE
                     TTRVVRESVPVRDVADSTASPGGRTVADIYKREGAGVVFVLAEGASESTSPFGPPGGG
                     GQASGSGFVVDKDGTIITNAHVVGGADEVQVRFEEDGDAVDAEVVASDPDTDVAILKV
                     DPDDADLQPLPLGDSSETQVGDPVVAIGNPFGYTRSVTTGIVSAKQRQIEAPSGFSIA
                     NVIQTDASINPGNSGGPLLDADGKVIGINSQIATGGSQGSVGIGFAVPIDTVKELLPR
                     LKRGDEIERAFLGIEMIGVTEEIARDLNLAADEGALIQDVVEGGPADEAGLRDGGTQL
                     GEQLSAGGDLIVEVDGDEIRTSDDVPAAIEDRKPGDEVEIVFYRGDERKTATVELGKR
                     PSSLNATEQQPDDGGGIPDLP"
     gene            1800..2447
                     /locus_tag="VHF88_08075"
     CDS             1800..2447
                     /locus_tag="VHF88_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX2161763.1"
                     /translation="MTRIKSCGITNLDDAELAVELGAWAVGLILWPGSRRACAPAAAE
                     QIGAALHRRCEIAGVFVNATLDEVALAADRYGLTLLQLHGDEGPAYCREAARRTGCKV
                     MKAVRVKDAAVVRGVEAFRTDYHLLDAYVPGTRGGTGETFAWDLLRHHRGSVPVVLSG
                     GLTADNVDAAIAAARPYAVDSASGTESAPGRKDPAKLQAFARAVEHADDREPAGA"
     gene            2444..3655
                     /gene="trpB"
                     /locus_tag="VHF88_08080"
     CDS             2444..3655
                     /gene="trpB"
                     /locus_tag="VHF88_08080"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX2161764.1"
                     /translation="MSTGAGQAPRGAGAPRPDSGGREREFGPYGGRFVPETLIPALDE
                     LDQAWIAACGDAGFAAELSALLRDYVGRPTPLYLARRLSAEVGGDVYLKREDLNHTGA
                     HKINNALGQALLAKRMGKRRIIAETGAGQHGVASATACALLDLECVVYMGSEDMRRQR
                     PNVERMGLLGARVEAVEAGARTLKEAVSAAIRDWVANAETTHYVIGSAVGPAPYPALV
                     RDLQRVIGDELRDQVQQIAGRLPDRVIACVGGGSNSIGAFAPFVLDSQVELVGVEAAG
                     EGIASGRHSAPLTTGARGVLHGAMSAVLQDDDGQVLEAHSISAGLDYPGSGPEHAWLR
                     DEGRVRYVAVEDDAAVDAFKRLSRLEGIIPALEPSHALAWLLANPNPGGVDALVLSGR
                     GDKDLAEVLAR"
     gene            3652..4458
                     /gene="trpA"
                     /locus_tag="VHF88_08085"
     CDS             3652..4458
                     /gene="trpA"
                     /locus_tag="VHF88_08085"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX2161765.1"
                     /translation="MSAVEELAGGQRIAAAFAGHGKRAALMPYLMGGHPSIDESLAAG
                     LAAADAGADLIELGIPFSDPLADGPVIHAAGTAALRDGVAPDDVLEICARLSDRIPVV
                     LMVYANVVLAGGTDRFVRRAAEVGAAGLIVPDMPQDEAASTRALCDASGLALVPLVAP
                     SSTADRVAEVGREARGFVYAVSLAGTTGERDELPPDLPRLVERVRAAAAVPVAVGFGI
                     STGAQAATVGDLADGVIVGSRVVRAAGEGGAEAVGRVVGELADALAERHL"
     gene            4471..4659
                     /locus_tag="VHF88_08090"
     CDS             4471..4659
                     /locus_tag="VHF88_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161766.1"
                     /translation="MIVLVTLTVGLVIWIVAWSFGIKAFDAFLFTALIAFVAAAVQIA
                     LPFVKQIVTGKPATPGEQ"
     gene            4710..5798
                     /locus_tag="VHF88_08095"
     CDS             4710..5798
                     /locus_tag="VHF88_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161767.1"
                     /translation="MAVLAAPLAACGGGGDDEDSTRVRGDTLTIYASLPSHGLDAPAG
                     DDAAVGMRRALADARGRAGGRSIRFVRMSSTRPGDRAWDPGTVEANADRAADDPTTIA
                     YLGELDRGGSAVSLPVTNRRQILQISPADALTSLTRTPPGRPRAGPERYYPGERQTFV
                     RLVSPDLVAARRIVAELREHGTRRLAIVTGDGVADHELEAMILGLIGEGQPRTVTRVG
                     APNADDDPARVAELTDELAEAAPQAIVYAADSEPRAWATLAALAARLPGVPVIEGPAL
                     AQPSAHVEGDGNCANAGVPAESALSRAARRRLETIRHQGGRDLGVEALLGYDAMRLAL
                     AAIDSGGPDRLDVVRAAKALAADYRLCG"
     gene            complement(5826..7274)
                     /locus_tag="VHF88_08100"
     CDS             complement(5826..7274)
                     /locus_tag="VHF88_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX2161768.1"
                     /translation="MAIPSAQFSLTLRVELPHESGTLSKVTAAISKVGGAIVAVDTVE
                     AGGDGTIREITIDCESQDHRGRVIKAVQSVQEAKLVETTDRTFELHRRGKIYTGLSSA
                     LKTRDDLSMAYTPGVARVCTAIAENRQKAFKYTIKANTVAVVTDGTAVLGLGDIGPEA
                     AMPVMEGKAMLFKEFADVDAFPIALDTKDPDKIVETVKLMAPTFGGINLEDISSPRCF
                     EIEQRLIEELDIPVFHDDQHGTAIVVLAALLNACRLTGRNIEDLSVSMAGAGAAGVAV
                     AKILMGAGITRVVACDRFGAIHTGREDYEAGSMNSPKRWLAENTNHERRDGSPAEVLE
                     GTDLFCGLSGPGIISAQDLQRMNAEPFVFAMANPNPEVRPEDAAPYVRVMATGRSDYP
                     NQINNVLAFPGIFRGALDVRARKISEEMKIAAAKGIAECIDDDELTEDYIIPSVFNRD
                     VAREVARVVAEEAEREGAVRLDDQTAELSLPG"
     gene            7342..10020
                     /gene="polA"
                     /locus_tag="VHF88_08105"
     CDS             7342..10020
                     /gene="polA"
                     /locus_tag="VHF88_08105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX2161769.1"
                     /translation="MPPQPPKELFLIDGNSLVYRAFFALPESIATSRGQPTNAIFGFA
                     SMLVKLLTEFGPKPTIVVWDAGASGRKEVYPEYKGDRPSRPDLLREQFPHFTPLVEAF
                     GYMNVSCAGYEADDVIATLARRAREHGTEVMIVTGDRDTFQLVEDGVRVMATGRGITD
                     TKIYDRQAVIDRYGIEPELIPDFYGLKGDTSDNIPGVPGIGDKTAATLLQQFGDLESI
                     LASVDQISGAKRKQNLVDHAEDARISKQLATSIRDVPVDAIDLDHLLAREPDRARLRE
                     VFREFELRDPLRRLEEALGDGEAAAPMPAASETVAVRVRRVEPAALATLSGDHPALWA
                     DRPEPPEGQLPGMGDDAPLTFAAYGGNKEALAGEAETLTEVVAAWGDRPLTVHDWKTL
                     TVSEQPVASWATADDDGGGPAISAPPLQHDTLVAAYLIDPARRSYPLDELASDLGLGA
                     EIQGATGEDRSKEAAEQAVLLHALAARQRETLDELGLTRLLEEVELPLVEVLVEMERA
                     GVRLDVGRVREIATRIGDEATALERRIWEAAGEEFTIGSPQQLAEILFTKLELSKKRR
                     GKTGFSTDARVLAAIRHEHEIIPMIEEWRELTKLKSTYLDAFPSLLGDDGRLRTTFSQ
                     VTAATGRLSSSNPNLQNIPVRTERGREIRACFVAEDGCRLISADYSQVELRLLAHIAG
                     EEVLKEIFRRGEDVHTATAAWILGDDGEGPVDPGTRSKAKMVNYGIVYGLSAFGLADR
                     LQIPQEEAQQFIDRYLERFPAVKAFIDSTVERATDEGFVATEFGRIRRIPELRARNRQ
                     TRMLGERLAVNMVIQGTAADIIKVAMVNSVRALRDAGLRTRLVLQIHDELLFEGPGSE
                     VADASTIVQREMAAAFEMDPPLDTDVGVGTNWLEAK"
     gene            10023..10469
                     /locus_tag="VHF88_08110"
     CDS             10023..10469
                     /locus_tag="VHF88_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2161770.1"
                     /translation="MDRWVAVVAMVAAGGLVAMQAPINSTLGKSVGTFAAASVSFAVG
                     LAVLVGITALFAGGFGEVANAADLRWYYLTGGLLGAAYVTTVLVAVRTLGAGGVTAAT
                     ITGQLTLAVIIDRLGWFGIDKQGFTFQRILGIALLAVGTFLVVRGK"
     gene            complement(10902..11258)
                     /locus_tag="VHF88_08115"
     CDS             complement(10902..11258)
                     /locus_tag="VHF88_08115"
                     /inference="COORDINATES: protein motif:HMM:NF022170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HEX2161771.1"
                     /translation="MAAWWLVLVTALIAHGCDESSRSANQGAENPGAVSRAELGADWP
                     LTVDRGVLSCDGPGVVFTTPDGSEYAVNGTAKGAGYADIEPIWREDPAYDLPDDGPPL
                     RVDISPLIERGLELCE"
     gene            complement(11270..11599)
                     /locus_tag="VHF88_08120"
     CDS             complement(11270..11599)
                     /locus_tag="VHF88_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX2161772.1"
                     /translation="MEKTIVEKAPPPETVTETVEAKPASAPKPKPKPAANLITVPDVV
                     GENHQYAQDTMQAAGLYNLDERDATGQGRLLLWDRNWVVVSQDPPAGARVSDNATVTL
                     SAKKDDE"
     gene            complement(11949..>13191)
                     /locus_tag="VHF88_08125"
     CDS             complement(11949..>13191)
                     /locus_tag="VHF88_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161773.1"
                     /translation="RRRLAAYGTSRIPRSFTLDGYAARPMPVRATKRGDERTPLSGDY
                     DVLICGASFAGLAVARELGGATRADGTPARVLMLDRYEIGERQTSACGIPTGWLEALG
                     LMGAHRQTFSELVVGTPHGTARWPLPWTFSTFDYPELCNLLAAQGDAEFETAKVDGRS
                     GNTVHTDRGDLTAPLIVDCLGWRRVLAGTDDDPAYQPPDAPLSRGLEVHPAGQGNDLA
                     IWIDRSIVPAGYGWSFPARDELRIGVGSFDPRFHVKDNTVKLAKDLGSEPIGYQGNWI
                     PHKLRDSVEDGIFFAGDSAGHCLPLTAEGIRTALYFGIACGRELRSVLVGEKTREQGL
                     GAYFAFNAEHEWKFRWMLRTQKLVPRIHPPLMNRIVGLMEKPALVDWSFRHYWNIASP
                     EIVTAAPSPTRRAPVPTSAAA"
BASE COUNT         1879 a         4647 c         4726 g         1939 t
ORIGIN      
        1 gcgaggcgac cgcgctgccg atcctgcgca aggacttcac cgtcgacccg tacatgctct
       61 acgaggcacg ggcggctggg gccgatgccg tgctgctggt cgtcgggtcg attgacgagg
      121 ctgagctggc cgagttgctg gccgcctcgc ggactctcga cctcgacgcg atcgtcgagg
      181 tccacgagcc cgacgagctc gagctggcgc tcgctgtcga cgccgacgtg atcgggatca
      241 acaaccgcga cctcgccgac ttcagcgtcg acgtcgctcg gacgttcgac ctgctgccgg
      301 atgtgcccgc tggcaaggtc gtggtgtcgg agtcgggcat cgtgcggcgc gagcagatcg
      361 aggagctcga gcgtgtcggc gtcgacgccg tgctcgtcgg cgagacggtc atgcgcgcgg
      421 acgatccaga ggcggccgtg cgagcacttg tcggccacga gcactcggtc gcaagcgatg
      481 gtccgtccgg cggcgtttag cctcgcttaa gcaccaccgc taaaactgcg ttccatgaac
      541 gagacgaccc cgccacagaa gcgctcatcg atttcgacct ttctggccgg ggcgcttggg
      601 ggcctgatcg tgctcgtcct cggcgccgcg ctgatcgcga ccgacgtgat cgacaccggc
      661 gacgagacga cgcgcgtcgt tcgcgagagc gtcccggtcc gcgacgtcgc cgactccacg
      721 gcgagccccg gggggcgcac ggtcgccgac atctacaagc gcgagggcgc cggcgtggtc
      781 ttcgtcctcg ccgagggcgc gagcgagagc acgtcgccgt tcggtccgcc cggcggcggt
      841 gggcaggcga gcggctccgg cttcgtcgtc gacaaggacg gcacgatcat caccaacgcg
      901 cacgtcgtcg gcggggccga cgaggtccag gtcaggttcg aggaggacgg tgacgccgtc
      961 gacgccgagg ttgtggccag cgatccagac accgacgtgg cgatcctcaa ggtcgacccc
     1021 gacgatgcgg acctgcagcc gctgccgctc ggggacagct ctgagacaca ggtcggcgac
     1081 ccggtggtcg cgatcggcaa cccgtttggc tacacgcgct cggtcacgac cggaatcgtc
     1141 tccgccaagc agcgccagat cgaggcgccg agcgggttct cgatcgcgaa cgtgatccag
     1201 accgacgcgt cgatcaaccc cggcaactcc ggcggcccgc tgctcgacgc cgacggcaag
     1261 gtgatcggca tcaactcgca gatcgccacc ggtggctcgc agggctcggt cggcatcggc
     1321 ttcgcggtgc cgatcgacac cgtcaaggag ctcctgccgc ggctcaagcg gggcgacgag
     1381 atcgagcgtg cgttcctcgg catcgagatg atcggggtga ccgaggagat cgcccgcgac
     1441 ctcaacctcg ccgccgacga gggcgcgttg atccaggacg tggtcgaggg cggccccgcc
     1501 gacgaggcgg gcctgcgcga cggcggcacc cagctcggcg agcagctctc tgccggcggc
     1561 gacctgatcg tcgaggtcga cggcgacgag atccggacct ccgacgatgt cccagcggcg
     1621 atcgaggacc gcaagccggg cgacgaggtc gagatcgtct tctaccgcgg tgacgagcgc
     1681 aagactgcca cggtcgagct cggcaagcgg ccgtcgtcac tcaacgccac cgagcagcag
     1741 cccgacgacg gaggcggcat acccgacctc ccataggacg ggcctgcgac gatggtcccg
     1801 tgacccggat caagtcgtgc ggcatcacca acctcgacga cgccgagctg gcggtcgagc
     1861 tgggagcttg ggcagtcggc ctgatcctct ggccgggaag ccggcgcgcc tgcgcgccgg
     1921 cggctgccga gcagatcggc gctgcgctgc accgccgctg cgagatcgcg ggcgtgttcg
     1981 tcaacgcgac gctcgacgag gtggcgctcg ccgccgaccg ctatggcctg acgctgctgc
     2041 agctgcacgg cgacgagggg ccggcgtact gccgcgaggc cgcccgccgc accggctgca
     2101 aggtgatgaa ggcggtgcgc gtcaaggacg ccgcggtcgt gcgcggcgtg gaggcctttc
     2161 gaaccgacta ccacctgctc gacgcctacg tgcccggcac gcgcggcggc accggcgaga
     2221 cgttcgcatg ggacctgctg cgccaccacc gcggtagcgt cccggtcgtg ctctcgggag
     2281 gcctgaccgc agacaacgtc gacgcggcga tcgccgccgc gcggccatac gccgtcgaca
     2341 gcgccagtgg caccgagtcc gcaccgggcc gcaaggaccc ggcgaagctg caggcgttcg
     2401 cgcgcgcggt ggagcacgcc gacgaccgag agcccgccgg cgcatgagca ccggggccgg
     2461 ccaggcgcca cggggggcgg gagccccccg acccgattcc ggggggcgcg agcgtgagtt
     2521 cggtccctat ggcggccgct tcgttccgga gaccctcatt ccggcgctcg acgagctgga
     2581 ccaggcctgg atagcggcct gcggcgatgc cggcttcgcc gccgagctga gcgcgctgtt
     2641 gcgcgactac gtcggccgtc cgacgccgct ttacctagcc cgtcgcctct cggcggaggt
     2701 agggggtgac gtctacctca agcgcgagga cctcaaccac acgggcgccc acaagatcaa
     2761 caacgcgctt ggtcaggcgc tgctcgccaa gcggatgggc aagcggcgga tcatcgccga
     2821 gaccggcgcc ggccagcatg gcgtcgccag cgcgaccgcc tgcgcgctgc tcgacctcga
     2881 gtgcgtcgtc tacatgggca gtgaggacat gcgccgccag cggccgaacg tcgagcggat
     2941 gggcctgctc ggcgcgcgcg tcgaggctgt cgaggccggc gcccggaccc tcaaggaggc
     3001 ggtgagcgcg gcgatccgcg actgggtggc gaacgccgag accacccact acgtcatcgg
     3061 ctcggcggtc gggccggcgc cataccccgc gctcgtgcgc gacctgcagc gagtgatcgg
     3121 cgacgagctg cgcgaccagg tccagcagat cgccggacgg ctgcccgacc gcgtgatcgc
     3181 ctgcgtcggc ggggggtcga actcgatcgg cgccttcgct ccgttcgtgc tcgacagcca
     3241 ggtcgagctg gtcggcgtcg aggcggcggg cgaggggatc gccagcggcc gccactcggc
     3301 tccgctgacg accggcgcac gtggcgtgct gcacggcgcg atgtcggcgg tgctccagga
     3361 cgacgacggc caggtgctcg aggcccattc gatctcagcc gggctcgact atcccggctc
     3421 ggggccggag cacgcctggc tgcgcgacga gggccgcgtt cgctacgtcg ccgtcgagga
     3481 cgatgccgcc gtggacgcct tcaagcggct gtcgcggctc gaggggatca ttccggcgct
     3541 cgagccctcg cacgcgctcg cctggctgct cgccaatccc aatcccggcg gcgtcgacgc
     3601 gctcgtgctg tcgggccgcg gcgacaagga cctcgccgag gtgctggcta ggtgagcgcc
     3661 gtcgaggagc tcgccggcgg gcagcggatc gccgcggcct tcgccggaca cggcaagcgg
     3721 gcggcgctga tgccatacct gatgggcggc catcccagca tcgacgagtc gctcgccgcc
     3781 gggctcgcgg ccgccgatgc cggcgccgac ctgatcgagc tggggatccc gttctcggac
     3841 ccgctcgccg acggcccggt gatccacgcc gccggcacgg ccgcgctgcg agacggcgtc
     3901 gccccggacg acgtgctcga gatctgcgcg cggctctccg accgcatccc cgttgtcctg
     3961 atggtctacg cgaacgtcgt cctcgcgggc gggaccgacc ggttcgtccg gcgggcggcc
     4021 gaggtggggg ccgcgggact gatcgtgccg gacatgccgc aggacgaggc cgcctcgacg
     4081 cgggcgctct gcgacgcgtc cgggctggcg ctcgtaccgc tggtcgctcc ctcgagcacg
     4141 gccgatcgag ttgccgaggt tggccgcgag gcgcgcggat tcgtgtacgc ggtctcgctg
     4201 gcggggacga ccggcgagcg cgacgagctg ccgcccgacc tgccccggct ggtcgagcgc
     4261 gtccgagcgg ccgccgccgt accggtcgcg gtcggcttcg gcatctcgac gggcgcgcag
     4321 gccgcgaccg tcggcgacct cgcggacggc gtcatcgtcg gcagccgggt cgtccgcgca
     4381 gccggcgagg gcggcgccga ggcggtcggc cgggtcgtcg gcgagctcgc cgacgccctc
     4441 gccgagcggc acctgtaatc tggcgcgccg atgatcgtcc tagtcacact caccgtcggg
     4501 ctcgtaatct ggatcgtcgc ctggtcgttt ggcatcaagg cgttcgacgc gttcctgttc
     4561 acggccttga tcgccttcgt cgccgccgct gtccagatcg ccctcccgtt cgtcaagcag
     4621 atcgtgacgg gcaagcccgc cacgcccggc gagcagtagc ggccgccgct cggaacccgg
     4681 cgccggtgcg ccgctctatt gcgatcctcg tggccgtcct cgcggctccg ctggccgcct
     4741 gcggcggggg cggcgacgac gaagactcga cccgtgtccg gggcgacacc ctgacgatct
     4801 acgccagcct gcccagccac ggactggacg caccggccgg cgacgacgcg gcggtcggga
     4861 tgcgacgcgc cctcgccgac gcgcgcgggc gcgcgggcgg acggtcgatc cggtttgtgc
     4921 ggatgtcgtc gacgaggccc ggcgaccggg cctgggatcc ggggaccgtc gaggccaacg
     4981 ccgaccgcgc ggctgacgac ccgacgacga tcgcctacct cggcgagctc gaccgcggcg
     5041 gctccgccgt gtccctgccg gtgacgaacc gcaggcagat cctccaaatc tccccggcgg
     5101 acgcgttgac cagcctgact cggacgcccc ccgggcgtcc gcgcgccggg ccggagcgct
     5161 actacccggg cgagcgccag acctttgtcc gcctcgtctc gccggacctc gtggcggcgc
     5221 gccggatcgt cgccgagctg cgcgagcacg gcacgcgccg gctggcgatc gtcaccggcg
     5281 acggtgtcgc cgaccatgag ctcgaggcga tgatcctcgg cctgatcggc gagggccagc
     5341 cgcggaccgt cacgcgggtc ggcgcgccga atgcggacga cgatcccgcg cgcgtcgccg
     5401 agctcaccga cgagcttgcc gaggcggcgc cgcaggcgat cgtctacgcc gccgactccg
     5461 aaccgcgcgc ttgggcgacg ttggcggccc tcgcagcccg gctgccgggc gtgcccgtga
     5521 tcgagggtcc ggcgctggca cagcccagcg cacatgtcga gggcgacggc aactgcgcta
     5581 acgccggcgt cccagccgag tcggcgctgt cgcgagcggc gcgccggcgg cttgagacga
     5641 tccgccatca gggcggacgc gaccttggcg tcgaggccct gctcggctac gacgcgatgc
     5701 ggctcgcgct cgcggcgatc gacagcggcg ggccggaccg gcttgacgta gtgcgcgctg
     5761 cgaaggcgct cgcggccgac taccggcttt gcggctagct gctgcggagg cagggccgcc
     5821 cggggctagc cggggaggct gagctcggcg gtctggtcgt cgagccggac ggcgccctcg
     5881 cgctcggcct cctcggcgac cacgcgcgct acctcgcggg cgacgtcgcg gttgaagaca
     5941 gaggggatga tgtagtcctc ggtcagctcg tcgtcgtcga tgcactcggc gatccccttc
     6001 gcggccgcga tcttcatctc ctcgctgatc ttgcgcgcgc gcacgtcgag cgcgccgcgg
     6061 aagatgcccg ggaaggcgag cacgttgttg atctggttcg ggtagtcgga gcgcccggtc
     6121 gccatcacgc gcacgtaggg ggcggcgtcc tcgggcctga cctcggggtt ggggttggcc
     6181 atcgcgaaga cgaacggctc cgcgttcatc cgctgcagat cctgcgcgga gatgatgcct
     6241 gggccggaca gcccgcagaa gaggtcggtg ccctcgagca cctcggccgg cgagccgtcg
     6301 cggcgctcgt ggttggtgtt ttcggcaagc cagcgcttcg gcgagttcat cgaaccggcc
     6361 tcgtagtcct cgcggccggt gtggatcgcc ccgaagcggt cgcaggcgac gacccgggtg
     6421 atgccggctc ccatcaggat cttcgcgacg gcgacgcccg cggcgcccgc gccggccatc
     6481 gagaccgaca ggtcctcgat gttgcggccg gtcagccggc acgcgttcag cagtgccgcc
     6541 agcacgacga tcgcggtgcc gtgctggtcg tcgtggaaga cggggatgtc gagctcctcg
     6601 atcagccgct gctcgatctc gaagcagcgc ggggaggaga tgtcctcgag gttgatcccg
     6661 ccgaacgtcg gcgccatcag cttgacggtc tcgacgatct tgtcggggtc cttggtgtcg
     6721 agcgcgatcg ggaaggcgtc gacgtcggcg aactccttga agagcatcgc cttgccctcc
     6781 atcacgggca tcgcggcctc gggcccgatg tcgccgaggc cgagcaccgc ggtgccgtcg
     6841 gtgacgaccg ccaccgtgtt ggccttgatc gtgtacttga acgccttttg gcggttctcg
     6901 gcgatcgctg tgcagacgcg cgcgacgcca ggggtgtagg ccatcgagag gtcgtcgcgc
     6961 gtcttcagcg ccgacgacag cccggtgtag atcttgccgc gccggtgcag ctcgaaggtg
     7021 cggtcggtcg tctcgacgag cttggcctcc tggacgctct ggaccgcctt gatcacgcgc
     7081 ccgcggtggt cctggctctc gcagtcgatc gtgatctcgc ggatcgtccc gtcgccgccc
     7141 gcctcgaccg tgtcgacggc gacgatcgcg ccgccgacct tgctgatcgc cgcggtcacc
     7201 ttgctcagcg tgcccgactc gtgcggcagc tcgacgcgga gggttaggga gaactgggcg
     7261 ctgggaatgg ccatgacggg aggcgaaaca tatcggccgc gccagtccgg ctcgggccgc
     7321 gcggctcagt agcctgggcg cgtgccgccg cagccgccca aggagctgtt cctgatcgac
     7381 gggaacagtc tggtgtaccg cgccttcttc gcccttccag agtcgatcgc cacctcgcgc
     7441 gggcagccga ccaacgcgat cttcggcttc gcgtcgatgc tcgtgaagct gctgaccgag
     7501 ttcggcccga agccgacgat cgtcgtctgg gacgccggcg cctcggggcg caaggaggtc
     7561 taccccgagt acaagggcga ccgcccgtcg cggcccgacc tgctgcgcga gcagttcccg
     7621 cacttcacgc cgctggtcga ggcgttcggc tacatgaacg tcagctgcgc cggctacgag
     7681 gccgacgacg tgatcgcgac gctcgcgcgc cgggcgcgcg agcacggcac cgaggtgatg
     7741 atcgtcaccg gcgaccgcga caccttccag ctcgtcgagg acggcgtgcg cgtgatggcg
     7801 accggccgcg gcatcaccga cacgaagatc tacgaccgtc aggcggtcat cgaccgctac
     7861 gggatcgagc ccgagctgat cccggacttc tatggcctca agggcgacac ctcggacaac
     7921 atcccaggcg tccccgggat cggcgacaag accgccgcga cgctgctgca gcagttcggc
     7981 gacctcgagt cgatcctcgc gagcgttgac cagatctccg gcgccaagcg caagcagaac
     8041 ctcgtcgacc acgccgagga cgcgcgcatc tcgaagcagc tcgcgacctc gatccgcgac
     8101 gtgccggtcg acgcgattga cctcgaccac ctgctcgccc gcgagcccga ccgcgcccgc
     8161 ctgcgcgagg tctttcgcga gttcgagctg cgcgatccgt tgcgccggct cgaggaggcg
     8221 ctcggcgacg gcgaggccgc cgcgccgatg ccggccgcca gcgagacggt cgccgtgcga
     8281 gtgcgccgcg tcgagccggc cgcgctggcg acgctgagcg gcgaccaccc ggcgctctgg
     8341 gccgaccgtc ccgagccgcc ggagggtcag ctgccgggca tgggcgacga cgcgccgttg
     8401 accttcgccg cctacggcgg caacaaggag gcgctggccg gcgaggccga gacgctgacc
     8461 gaggttgtcg ccgcctgggg cgaccggccg ctgaccgtcc acgactggaa gacgctgacc
     8521 gtctccgagc agcctgtcgc ctcgtgggcg acagccgacg acgacggcgg cgggccggcg
     8581 atctccgcgc cgccgctcca gcacgacacg ctcgtcgcgg cgtatctgat cgaccccgca
     8641 cgccgcagct atccgctcga cgagctcgcg agcgatctcg ggctcggcgc ggagatccag
     8701 ggcgcgacgg gggaggaccg ctcgaaggag gcggccgagc aggccgtcct gctccacgcg
     8761 ctcgccgcgc gccagcgcga gacgctcgac gagctcgggc tgacgcggct gctcgaggag
     8821 gtcgagctgc cgctggtcga ggtgctggtc gagatggagc gcgccggcgt acggctcgac
     8881 gtcggtcgcg tgcgcgagat cgccacccgg atcggcgacg aggcgaccgc gctcgagcgc
     8941 cggatctggg aggcggccgg cgaggagttc acgatcggct cgccgcagca gctcgccgag
     9001 atcctcttca ccaagctcga gctgtcgaag aagcggcgcg gcaagaccgg cttctcgacc
     9061 gacgcccgcg tgctcgcggc gattcgccac gagcacgaga tcatcccgat gatcgaggag
     9121 tggcgcgagc tgaccaagct caagtcgacc tacctcgatg cgttcccctc gctgctcggc
     9181 gacgacggac ggctgcgcac cacgttcagc caggtgaccg ccgcgaccgg ccgcctctcg
     9241 agcagcaacc ccaacctcca gaacatcccg gtgcgcaccg agcgcggccg cgaaatccgc
     9301 gcctgcttcg tcgccgagga cggctgccgc ctgatctccg ccgactactc ccaggtcgag
     9361 ctgcggctgc tcgcccacat cgccggcgag gaggtgctca aggagatctt ccgccgcggc
     9421 gaggacgtcc acaccgccac cgccgcctgg atcctcggcg acgacggcga gggccctgtc
     9481 gatcccggca cccgctcgaa ggccaagatg gtcaactacg ggatcgtcta cggcctctcc
     9541 gccttcggcc tcgccgaccg cctgcagatc ccccaggagg aggcccagca gttcatcgac
     9601 cgctacctcg agcgcttccc cgccgtcaag gcgttcatcg actcgacggt cgagcgcgcc
     9661 accgacgagg gcttcgtcgc gaccgagttc ggccgcatcc gccgcatccc cgagctgcgc
     9721 gcccgcaacc gccagacccg catgctcggc gagcggctcg cggtcaacat ggtgatccag
     9781 ggcacagccg cggacatcat caaggtggcg atggtcaatt cggtccgcgc gctgcgcgac
     9841 gccggcctgc gcacccggct cgtgctccag atccacgacg agctgctctt cgagggcccg
     9901 gggtcagagg tcgcggacgc ttccacgatc gtgcagcgcg agatggccgc cgccttcgag
     9961 atggatccgc cgctggacac cgacgtcggc gtcgggacca actggctcga ggccaagtag
    10021 ccgtggaccg ctgggtcgcc gtcgtcgcga tggttgccgc cggcggcctc gtcgccatgc
    10081 aggccccgat caactccacg ctcggcaagt ccgtcggcac cttcgccgcc gcctccgtct
    10141 ccttcgcggt cggcctcgcc gtcctcgtgg gcataacggc gctgttcgcc ggcggcttcg
    10201 gcgaggtggc caatgccgcc gacctgcgct ggtactacct cacgggcggt ctgctcggag
    10261 ccgcctacgt caccaccgtc ctcgtcgcgg tccggacgct cggagccggc ggcgtcaccg
    10321 cggccacgat caccggccaa ctgacgctcg ccgtcatcat cgaccgcctc ggctggttcg
    10381 gcatcgacaa gcagggcttc accttccaac gcatcctcgg catcgccctg ctggctgtcg
    10441 gcaccttcct ggtcgtccgc ggcaagtagc ggttcttcag gctagggaca gaccgtgcag
    10501 gccgcgggtc ctgtctccga ttttggttca cgtgctcgtt cctgcgcgcg cgaacgcaaa
    10561 atcgtcgcca cccgcggatc agcatgcaaa gcctgcgtga cagcgtgcga cgaatcggta
    10621 actgcggttc gacgccgaga ctcggcgggc ttagggtcgg cgagcgcgga gggttgaagc
    10681 agacaggcgt gctgcacgta attgatacct acgatgttcg gctagccgcc gaatccggtt
    10741 cgagtccggc gccctccgcc gacatagcca gaggcgaggc cgaagctgac gcgaagcgct
    10801 aacgtgggcg ctgggtatca attgcgtgtg gggtggctcc ccacttcaac agtcttcggc
    10861 ccgcccctgg cgggtcgtcg gcttttcaag ggcggaggtg gctattcgca gagctccaag
    10921 ccgcgctcga tcagcggaga gatgtccacg cgaagcggcg gtccgtcgtc cggtagatcg
    10981 taagctgggt cctctcgcca aatcggctcg atgtccgcgt agccggcgcc cttagccgtg
    11041 ccgttcactg cgtactcaga gccgtcgggc gttgtgaaga cgacgcctgg gccgtcgcaa
    11101 ctcagcactc cgcgatcgac ggtgagcggc cagtcagctc cgagctcggc tcgggacacg
    11161 gcgcctgggt tctccgctcc ctggttggcc gacctcgacg actcgtcgca gccgtgcgcg
    11221 atcaacgcag tgaccagcac cagccaccag gctgccatgc gatgcacggc tactcgtcgt
    11281 ccttcttcgc ggacagcgtg actgtcgcgt tgtcgctcac ccgggcgccc gccggaggat
    11341 cctgggacac gacgacccag ttgcggtccc acagcaacag acggccctgg ccagtagcgt
    11401 cgcgctcatc caagttgtag agcccggctg cctgcatcgt gtcctgtgcg tactggtggt
    11461 tctcaccaac gacgtcgggc accgtgatga ggttcgccgc cggcttgggc tttggctttg
    11521 gagccgaggc cggcttggcc tcgacggtct cagtgaccgt ctccggcggc ggggccttct
    11581 caacgatcgt cttctccacc gtttgtgccg cctcatccgt gccgcagcct gccgacgcta
    11641 caagcgcgac cgtagccgcg cacgcgagct tcctcaaggg tgaccctccc gagtcgagac
    11701 ttttctgtaa ttcggcgacc ctatccgtga tttgttccat tcgccagtac acgggtcttc
    11761 cgcgggttgc gacggtggcg ggattcgcgc gcaggcgcag caggcgcgca gcccctgcca
    11821 actgtccagg tggagcgccc tcagccgcag ggagcgctcg gcacggggcg gagtcgccgc
    11881 ggagccgagc acggaaaccg ccaccggaga cgggacccgc ggcctgcgcg gcttagccct
    11941 agcctgagct aggcagcggc tgaagtgggg accggagccc ggcgtgtcgg tgatggcgca
    12001 gccgtgacga tctccggtga ggcgatgttc cagtagtggc ggaaggacca gtcgacgagg
    12061 gctggcttct ccatcagccc gacgatccgg ttcatcagcg gcggatggat gcgcgggacg
    12121 agcttctggg tgcgcagcat ccacctgaac ttccactcgt gctcggcgtt gaaggcgaag
    12181 taggcgccga gtccctgctc gcgggtcttc tcgccgacga gcacggagcg cagctcgcgg
    12241 ccgcaggcga tgccgaagta gagggcggtg cggatgccct cggcggtcag tgggaggcag
    12301 tggccggcgg agtcgccggc gaagaagatg ccgtcctcga cggagtcgcg cagcttgtgc
    12361 gggatccagt tgccctggta gccgattggc tcggagccga ggtccttggc gagcttgacg
    12421 gtgttgtcct tgacgtggaa gcgcgggtcg aaggagccga cgccgatccg cagctcgtcg
    12481 cgcgcgggga agctccagcc gtagccggcg gggacgatcg agcggtcgat ccagatcgca
    12541 aggtcgttgc cctggccggc gggatggacc tcgaggccgc gcgagagcgg ggcgtcgggc
    12601 ggctggtagg cgggatcgtc gtccgtgccg gcgagcacgc ggcgccagcc gaggcagtcg
    12661 acgatcagcg gtgcggtcag gtcgccgcgg tcggtgtgga cggtgttgcc gctgcggcca
    12721 tcgactttgg ccgtctcgaa ctcggcgtcg ccctgcgcgg cgagcaggtt gcacagctcg
    12781 gggtagtcga aggtcgagaa cgtccaggga agcggccagc gcgccgtgcc gtggggcgtg
    12841 ccgacgacga gctcggagaa ggtctggcgg tgggcgccca tcaggccgag cgcctctagc
    12901 cagccggtcg ggatgccgca ggcggaggtc tggcgctcgc cgatctcgta gcggtcgagc
    12961 atcagcacgc gggccggcgt cccgtcggcg cgggtggcgc cgccgagctc gcgggcgacc
    13021 gcgaggcccg cgaagctggc gccgcagatg aggacgtcgt agtcgccgga gagcggggtc
    13081 cgctcgtcgc cgcgcttggt tgctctgacc ggcatcggac gggcagcgta gccgtcgagc
    13141 gtgaaggagc gcgggatgcg gctcgtgccg tacgccgcta acctacgccg t
//
LOCUS       DATABV010000182         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1905400, whole genome shotgun sequence.
ACCESSION   DATABV010000182 DATABV010000000
VERSION     DATABV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905400"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VHF88_08130"
     CDS             <1..1059
                     /locus_tag="VHF88_08130"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX2161774.1"
                     /translation="GLILSGGKGTRLRPITHTSAKQLVPVANKPVLFYGIEAMAAAGI
                     EEVGIIIAPETGDEIRTVAGDGSAFGVRIEYIEQDAPLGLAHAVLTAEPFIGDSPFVM
                     YLGDNLLRDGITELTETFASEQPDALILLTPVPDPEHYGVAELSDGSVARLVEKPANP
                     QSDLALVGVYMFTPSIFEAAKAIEPSARGELEITDAIQWLVDGGRRVDPHIVHGWWKD
                     TGQVQDMLEANRLILDDIEERHDGEVVDSRVEGRVCIEAGARLERATVRGPAIIGAGA
                     KIVDAYIGPYTAIGDRVTISHAELEHSIVLADSSICDLESRVEASLIGRNVNIGRGPK
                     MPRAYRFVVGDNAEISIL"
     gene            complement(1141..>1902)
                     /locus_tag="VHF88_08135"
     CDS             complement(1141..>1902)
                     /locus_tag="VHF88_08135"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161775.1"
                     /translation="GDNVAFHAYRAGRGILGGRQLHMAPLTWRPDGWPAIGDGRPLPP
                     AAGALPVTFDDAFGGPALAPEWEWPGTHPPRIRVDGGLTLRAPVRSTNRAISPRVLAQ
                     RFDAGLLTRRLAGDRFTVTAVVDRASVRGRELAGVAVTRGGPFDIGGRSIGIAIEARN
                     RVDGEFAAITEWRRGARPTERGNRVQLRGRFAHLRIVASGRRYKFYASSDGVRWLPVG
                     PHWRSPVDETARVGLTVGGQRGASARFTRATLTEP"
BASE COUNT          285 a          673 c          707 g          237 t
ORIGIN      
        1 ggcctgatcc tgtccggcgg caagggcacg cggctgcggc cgatcaccca cacgagcgcc
       61 aagcagctcg tccccgtcgc gaacaagccg gtgctcttct acggcatcga ggcgatggcg
      121 gcagcgggga tcgaggaggt cgggatcatc atcgcgccgg agaccggcga cgagatccgg
      181 accgtcgccg gcgatggatc ggccttcggc gtgcggatcg agtacatcga gcaggacgcg
      241 ccgctcggac tcgcccacgc cgtgctgacg gccgagccgt tcatcggcga ctcgccgttc
      301 gtgatgtacc tcggcgacaa cctgctgcgc gacgggatca ccgagctgac cgagaccttc
      361 gcgagcgagc agcccgacgc gctgatcctg ctgacgccgg tgccggatcc cgagcactac
      421 ggcgtcgccg agctgtccga cggcagcgtc gcgcggctcg tcgagaagcc ggcgaacccg
      481 cagagcgacc tcgcactggt cggcgtttac atgttcacgc cgtcgatctt cgaggcggcg
      541 aaggcgatcg agccttcggc gcgcggcgag ctcgagatca ccgacgcgat ccagtggctg
      601 gtggacggcg gccgccgcgt cgacccgcac atcgtccacg gctggtggaa ggacaccggc
      661 caggtccagg acatgctcga ggccaaccgc ctgatcctcg acgacatcga ggagcgccac
      721 gacggcgagg tcgtcgactc gcgcgtcgag ggacgggtct gcatcgaagc gggcgcgcgg
      781 ctcgagcggg cgacggttcg cggcccggcg atcatcggcg cgggagcgaa gatcgtcgac
      841 gcatacatcg ggccgtacac cgcgatcggc gaccgcgtga cgatctcgca cgccgagctc
      901 gagcactcga tcgtcctcgc cgactcgtcg atctgcgacc tcgagtcgcg cgtcgaggcg
      961 agcctgatcg gccgcaacgt caacatcggc cgcgggccga agatgccgcg cgcgtaccgg
     1021 ttcgtcgtcg gcgacaacgc cgagatttcg atcctctagt acgacgcggc ggccgatgat
     1081 ggattggcac gccctggcga ggccaatcca tcattgggcg cggacacgcc gtggcgccga
     1141 ctagggctcg gtcagcgtcg cgcgcgtaaa gcgggcgctg gcgccgcgct ggccaccgac
     1201 ggtgaggccg acgcgggcgg tctcgtcgac gggggagcgc cagtgggggc cgacgggcag
     1261 ccagcgcacg ccgtcggagc tggcgtagaa cttgtagcgg cggccgctgg cgacgatccg
     1321 caggtgggcg aagcgaccgc gcagctgcac gcggttgccg cgctcggtcg ggcgggcgcc
     1381 gcggcgccac tccgtgatcg ccgcgaactc gccgtcgacc cggttgcggg cctcgatcgc
     1441 gatgccgatc gaccggccgc cgatgtcgaa cggaccgccg cgggtgacgg ccacgccggc
     1501 gagctcgcgc ccgcgaaccg acgcgcggtc gacgaccgcg gtgacagtga agcggtcacc
     1561 ggcgaggcgg cgcgtcagca gccccgcgtc gaagcgctgc gcgagcacgc gcggcgagat
     1621 cgcccggtta gtcgagcgga ccggcgcgcg cagcgtcagg cccccatcga cgcggatccg
     1681 cggcggatgc gtgcccggcc actcccattc gggcgcgagg gccgggccgc cgaacgcgtc
     1741 gtcgaaggtg accggcagcg ccccagccgc cggcggcagc ggccggccgt cgccgatcgc
     1801 cggccagccg tctggccgcc aggtcagcgg cgccatgtgc agctggcgcc cgccgaggat
     1861 tccccgtccg gcccggtagg cgtggaaggc gacgttgtcg cc
//
LOCUS       DATABV010000183        25630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1663778, whole genome shotgun sequence.
ACCESSION   DATABV010000183 DATABV010000000
VERSION     DATABV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 25630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25630
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663778"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VHF88_08140"
     CDS             <1..533
                     /locus_tag="VHF88_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2161776.1"
                     /translation="GVPGSADRTELIDRIAAIAHDCAARAIGEPDAVVVCAGVEASSW
                     DDLAIGLRSTDVVLAGAVDMPARPWHDVAAPDLERVLWSLRDRPQLRDFAATRLAPLV
                     EHDARRSTKLLDTLRVLCAAGGRRAEAARALGIERQSLYHRLGRIEQLLDVDLSAGGT
                     LFELELALRVRDIGSA"
     gene            530..1468
                     /locus_tag="VHF88_08145"
     CDS             530..1468
                     /locus_tag="VHF88_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2161777.1"
                     /translation="MSRRRPLVGYAAALTAVTLWSLNASVSRTLLDGGLSASRLAELR
                     TAGTFALLLVAIALVRPRLLRVPIGEIRPLVALGVFGIGLLYVSYSLAVERIKIGVAL
                     TIEYLAPALILIWLALVRRRRLPAELVAAIVLALVGCAAMVGSYGSLDGVGVAAALAA
                     AFGYAFYLLTAERLSGRYHPATTLVWAFGAGTLVLFAVQPPHSFPFERLEGSADVALA
                     VEVIVVGTLLPFALMLLAVRHVTAIRIAVVGASEPALSALFAYLIHDERLSVVQILGG
                     LLLLGAVVWVQVQRPNLPAELAAAGAEPRRQPAGRA"
     gene            complement(1512..3494)
                     /gene="acs"
                     /locus_tag="VHF88_08150"
     CDS             complement(1512..3494)
                     /gene="acs"
                     /locus_tag="VHF88_08150"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX2161778.1"
                     /translation="MDASAGTTELERRIAELLDRESFEPPAEFVERALLTDPSIYEEA
                     ERDPVGWWVRQAEDLHWFERWDAVLDESEAPFYRWFEGGKLNASYNCLDRHVEAGLGD
                     RVAFHWRGEEGEERDVTYAELLRDTQRLANALKDRGVGPGDVVGIYLPMVPEVVVAML
                     ACARIGAPHNVVFGGFAPESVRERMEFSEAKALITADGARRKGKTAPVKPAVDAFLDS
                     LPAIETVVVVRNTGNDVDMRGGRDVWFDEALAEAAPDCPATPLDAEHPLYVLYTSGST
                     AKPKGILHTTGGYLTGVAWTHKHLFDLKPEEDVYWCSADVGWVTGHSYIVYGPLLNGA
                     TSVMYEGAPDYPDKDVWWEICARYGVTLFYTAPTAIRACMKWGAEHPARHDLSKLRLL
                     GTVGEPINPKAWVWYHVVIGGERCPIIDTWWQTETGHVLISPIPGITAAKPGSATRPL
                     PGIDAAVVDQDGYEADGQGLLVLRRPWPGMLRTLYKDPERFEETYFSKFGPTTYFVGD
                     AARKDEDGFFWVIGRVDDVINVSGHRLSTAEVESALVSHPRVAEAAVIGQQDEDTGQS
                     ICAFVTLEGDQDGDDDRIAELREHVAQRIGKLARPKRIVWADDLPKTRSGKIMRRLLR
                     DIAEGRELGDVTTLRDPDVMSQLEQKVAARRDDAEA"
     gene            complement(3540..3610)
                     /locus_tag="VHF88_08155"
     tRNA            complement(3540..3610)
                     /locus_tag="VHF88_08155"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3576..3578),aa:Cys,seq:gca)
     gene            complement(3679..5523)
                     /locus_tag="VHF88_08160"
     CDS             complement(3679..5523)
                     /locus_tag="VHF88_08160"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX2161779.1"
                     /translation="MSATEEQTPAVSPMSEMTAPPTQNKKLVAWVEEIAALTKPASIH
                     WCDGSAEEYDRLCQTLVDAGTFERLSDAKRPNSYLALSDPGDVARVEDRTFICSEDEA
                     DAGPTNNWRAPAEMRETLDDIFDGCMEGRTMYVVPFSMGPLGSPIAHIGIELTDSPYV
                     AVSMRIMTRMGQGALDVLGADGEFVPCVHSVGMPLTDGVEDVPWPCNADNKYIVHYPE
                     TREVWSFGSGYGGNALLGKKCFALRIASVMARDEGWMAEHMLILKLTSPQGESKYVTA
                     AFPSACGKTNLAMLIPTLEGWTVETVGDDIAWMKFGADGRLYAINPEAGFFGVAPGTG
                     EKTNPNAMATIERNTVFTNCAKTDDGDIWWEGMTDEAPAHLIDWHGNDWTPESDKPAA
                     HPNARFAVPAAQDPAIAPEWEDPAGVPIDAMLFGGRRSTVVPLVTEARDWEHGVFLGA
                     TMSSETTAAAAGQVGQLRFDPFAMLPFCGYNMADYMGHWLKIGEREGADLPKMFYVNW
                     FRKDDDGRFLWPGFGENSRVLEWVFRRCNGEGKAAETPFGLVPDASDLNTDGLDIDAA
                     DLDELLRVDPEALKAQLPQVKEHLAKFGDQLPAGIRAQLDDLEQRLGA"
     gene            5666..6097
                     /locus_tag="VHF88_08165"
     CDS             5666..6097
                     /locus_tag="VHF88_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX2161780.1"
                     /translation="MPTRTGSGEWQGDLKGGAGQLEVGDGVFSGPYSAKSRFEEGDGT
                     NPEELIAAAHAACFSMALSNILSSDGHIPDSVRTTAEIELRSPDTGPPIRKITLRTEG
                     RVPGIDADAFSEYAERAKDECPVSVALAGVPEMEVEATFEA"
     gene            6156..6229
                     /locus_tag="VHF88_08170"
     tRNA            6156..6229
                     /locus_tag="VHF88_08170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6190..6192,aa:Gly,seq:gcc)
     gene            complement(6357..6635)
                     /locus_tag="VHF88_08175"
     CDS             complement(6357..6635)
                     /locus_tag="VHF88_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161781.1"
                     /translation="MRTAHRRRFHRDRIVRKRQNAYRRLQLDRGPKLPERVLPDGRLE
                     DRQAYLACGRPRCGLCHSGKRWHRGANRARALDEWRRLELEGWDEIVT"
     gene            complement(6720..7007)
                     /locus_tag="VHF88_08180"
     CDS             complement(6720..7007)
                     /locus_tag="VHF88_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX2161782.1"
                     /translation="MEVVNIHIGPLVFDHADYDSDGDVLYLHRGAPQPAEGEETPEGH
                     VLRFAPGTDQIVGLTIVNARWLLDRDRRLVVTIPEKVEASAAELAPGLAAA"
     gene            7119..8003
                     /locus_tag="VHF88_08185"
     CDS             7119..8003
                     /locus_tag="VHF88_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2161783.1"
                     /translation="MRPAGAVLKRTLLSFYDDQMTHHAAALTYYSLMSLFPALLLGLS
                     LLGLLGQYPETYDAVMGYLREVSPESVIEPLDDSLRSALQSKGTAAASLTISVVVTLY
                     GTTGALEAARRALNVVFEVDGGRSFLQRKALDVASTFVLMALILASVVMAFVGGAFAE
                     DLLGFVGLGPTAAAIWSIARWPGALLIAMLVFALVYYVTPDVKQRAFRWITPGAVVGV
                     AVWLIASLGLSAYLSYIADVAAVYGAFTGAIVLVGWLWLTNVAMLLGAELNAEIERQK
                     ELAEGVPERETLNLPARG"
     gene            complement(8047..8232)
                     /locus_tag="VHF88_08190"
     CDS             complement(8047..8232)
                     /locus_tag="VHF88_08190"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX2161784.1"
                     /translation="MADGPSNIDEAKGRLKQGAGDLTGDDDLKREGKADEGAGKVKEG
                     IDKATDKVKGLLNRDDK"
     gene            8330..8677
                     /locus_tag="VHF88_08195"
     CDS             8330..8677
                     /locus_tag="VHF88_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161785.1"
                     /translation="MTDDRERDPLVDEQAEAAAREAAEIGGPDPTPGVDPAERPRVEG
                     GGGYAEGFEQAEEQLQRNASHEDRGADPIGDAGTDERADVGAEYGEADHAGAQGEDPG
                     SQSDASEPEDVGS"
     gene            8724..9182
                     /locus_tag="VHF88_08200"
     CDS             8724..9182
                     /locus_tag="VHF88_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161786.1"
                     /translation="MAYSTAEGRQELLDGLAGAIDELGYALASLGAAYEQLDQATADK
                     LEEELFGPVQRAYGRAKRTYGAFAQRAGLEPARFDMQSAGLPSTGAKGFVEHAVEGIS
                     SAGGRLATLQDSPVLLEVGDAELRQGLTEIRESIADLPHVARELLRRLGR"
     gene            complement(9171..9428)
                     /locus_tag="VHF88_08205"
     CDS             complement(9171..9428)
                     /locus_tag="VHF88_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX2161787.1"
                     /translation="MAYVYLLRCADDSLYCGWTTDVRRRLGEHEAGRASRYTRTRRPV
                     ELAAVFEAADRSAALREEARIKRLPRAAKLQLIGRSAALPA"
     gene            9485..10249
                     /locus_tag="VHF88_08210"
     CDS             9485..10249
                     /locus_tag="VHF88_08210"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX2161788.1"
                     /translation="MPRFVDLSAPIRQDPAELPELLRTDIAYTDHAGGARDIEALTGV
                     TPDLLRDGEGWTVETFTNFGTHSSTHVDAPWHYNSTIDGRRAQSIDELPLDWFFRPGV
                     VLDMTAKADGDPVTVGEVEAELERIGHELAERDIVLVRTGRDAFYDDPGYMALGPGVT
                     VEATHWLYERGVRVMGIDAWGWDAPLWMQAEEAKRRGERGIFWAAHQAGLAYSQIERL
                     FNLGELPPTGFTIACFPLKVVGGSAGPTRAVAILDE"
     gene            complement(10261..12489)
                     /locus_tag="VHF88_08215"
     CDS             complement(10261..12489)
                     /locus_tag="VHF88_08215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2161789.1"
                     /translation="MHARPDSQAAHPQARVWMLAEERSKQELVFAFPFDEQLNEAVKQ
                     LPGRWFDWRRKHWRVPAEPGAAQPVGELLERFPELLVEPDVIAWLTDSERWRALVSVV
                     IHSGAGAFVVRTVSGELPATGLEGGIATGEDRLVFPFDAEAAARLAQLDGARIDDLAK
                     ACIRDLAAGRTPPPAELGVEIGEDGEPELTLYTVWDPAVARDYKQLPEAKPVPRSARV
                     FNRDAAWGVAVPADPALARDLAGFVANHPGVGLDDRARDLLDELIEEHDRASATVALS
                     YADDAELDGVTLGGELHPFQRAGVRYALARRQTFIADEQGLGKTVQALAALEADDAFP
                     ALVVCPASMKLTWEREAGIWLPERRVAVLDGRTDATWDEAAEQAEIVVLNYDILEAHL
                     SRLLDSGPRALILDESHYVKNPRAGRTKAALELAGGLPDDSLRLALTGTPILNRPDEL
                     IAQLRVLGRLREFGSGARLSRRFRTAGSDDRLHWNLRAVCYVRRTKQQVLPQLPSKRQ
                     DTVPVLLSNEHEYRLAEEDVIAWLQTLPLDLGTLDAKIAAALRAEQLVRLNNLRQLAA
                     AGKLPTALAWIADFLESGEPLVVFAEHIAIQKAIMERFPGAAHILGADTTQARQRAVD
                     AFQREDGPQLIVCSMRAASQGITLTRASNVAFLELDWTPARHDQAEDRLHRIGQESAV
                     TAWYLLAPNTIDETMAELLQRKRSLIDAVTDGQVHDEERLVDAVVRELRGRPFRHLRV
                     VA"
     gene            complement(12537..12995)
                     /locus_tag="VHF88_08220"
     CDS             complement(12537..12995)
                     /locus_tag="VHF88_08220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX2161790.1"
                     /translation="MTNRADPAEPGASAAPRKVVVNVDGGARGNPGPAAVGAVATDPD
                     GGVLAERGTYIGETTNNVAEYRALLLGLELARELGASEVEVINDSELVAKQISGAYRV
                     KHAGLKPLYLEAMAALRAFDSWQVRSVRRESNTRADALVNAALDAELQAR"
     gene            complement(12992..13198)
                     /locus_tag="VHF88_08225"
     CDS             complement(12992..13198)
                     /locus_tag="VHF88_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161791.1"
                     /translation="MIYCVVPRALEDELYDKLVEHYSDEPNVEVIVDRRAQGGTPGSH
                     DPMTDRRRVRPKGSFPPIDAPPGG"
     gene            complement(13195..13962)
                     /locus_tag="VHF88_08230"
     CDS             complement(13195..13962)
                     /locus_tag="VHF88_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161792.1"
                     /translation="MKKELRPFHDPVSCDVCGRTILKGERIEHFLAPGGHRNLVCELC
                     FVRAEHAGWIRESSADQIPTRAGRPQERRQIFGRLRRRREGAVDGPRVEPGTAGAYAE
                     PASAADAHDSAAVEARAVDTAAEEPATPPRRREPPQDPRHVRAVPTTAEVKVERALEL
                     FNGSEHQRTVAGLTRTLGFPWATAVPDVAAPSAVTVVVAWELSWYRYRVDLGDTAEPV
                     ALQEKGDELNDIDEALRRWNAGLDEEGRLVMEGGAVR"
     gene            complement(13959..14309)
                     /locus_tag="VHF88_08235"
     CDS             complement(13959..14309)
                     /locus_tag="VHF88_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2161793.1"
                     /translation="MTTTADIAIERLGEWIVARVGGELDMTNCSYVRDELMRSIPGAA
                     DGLVVDLSETIYLDSAAIELLFELARRLGRHRQDLRIAMPGDSPLRRVLELTDVRAVA
                     PVHPTVDAAVASSQ"
     gene            complement(14320..15114)
                     /gene="whiG"
                     /locus_tag="VHF88_08240"
     CDS             complement(14320..15114)
                     /gene="whiG"
                     /locus_tag="VHF88_08240"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX2161794.1"
                     /translation="METNLKAIELKDLWSRYKQDGDQQARERLVLAYSPLVKYVAGRM
                     SSGLPAHVEEADLISYGLLGLISAIERFDLEREIRFETFAVTRIKGSIIDELRSLDWV
                     PRSVRAKAREIEQTNAKLENKLQRAPTDAEMSDAMGVTQVEFQESLVKIANSSVVALD
                     ELWTVSDSSGDQVSLLDTIPDPHAADPARELDLTDMKDSLADAIARLPEREKLVIALY
                     YYENLTLREIGEVLGVTESRVSQLHTKAVLRLKSKLQGDALTEAVE"
     gene            complement(15131..16057)
                     /locus_tag="VHF88_08245"
     CDS             complement(15131..16057)
                     /locus_tag="VHF88_08245"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX2161795.1"
                     /translation="MTKKTDTPPNPSWARTLRAFDTALRTRGLGEQTRRAYGSDLGQL
                     AEWASAQELAPGDVDARALRRFAGVLSERGNSRATVARKLAAFRAFYRVLIERGELEA
                     SPADLVASPKRDSDLPIVLSRAEVDRLFDSLPASTPLELRDRALFELAYASGLRAAEL
                     VDLDVDSLDRDGEQLRVEGKGAKTRIVPAGELAWRAVDAYLERGRPELAAAGDGRRDP
                     ALFLSRTGRRLATTDVRRRLRVALRHAGLQAAASPHALRHSFATHLLDGGADLRAIQE
                     LLGHSSLSTTQTYTRVESKRLRKAYAQAHPRA"
     gene            16038..16322
                     /locus_tag="VHF88_08250"
     CDS             16038..16322
                     /locus_tag="VHF88_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161796.1"
                     /translation="MSVFFVNTQDGRVATRSQLVDAELADDDHKPVAPWHPIQGPGGE
                     STLWYAVLRKRERGVFIGTLCIRHSERQASLEAAGWEEVAVDAIGVAQPM"
     gene            complement(16310..17341)
                     /locus_tag="VHF88_08255"
     CDS             complement(16310..17341)
                     /locus_tag="VHF88_08255"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEX2161797.1"
                     /translation="MKSKAFTFVAVLVVLLIAGSVAVYAYDSSRSDRIAEGVTVGGVA
                     VGGLSADEARVKVRRQVAARIEKPIAVTYGKTRFTLSAEDAGIRADVGGMVDDAIEAS
                     RDDNVLARVTRDLRGDEEDVEVSVSATYSPKAAQALVTRVGDKLDREPKDATLDFPSL
                     DAVKEQDGIAVKRGQLRRLIGSALLSPSSRTVEAPVKATKAEVTRDELAKEYPTVLVA
                     DHASYELRLYKDLKLVKTYTVAFGAAGYDTPRGLYSIQNKAVDPAWSVPNSDWAGDLA
                     GTVVPGGSPENPLKARWLGIYDGAGIHGTDDIGSLGTAASHGCIRMAVPDVIDLYDRV
                     EVGAPIYIG"
     gene            17495..18499
                     /locus_tag="VHF88_08260"
     CDS             17495..18499
                     /locus_tag="VHF88_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2161798.1"
                     /translation="MGRLIIPPPPARRRDAVRADGDYGVSDRPDWRAVDWPAHLRTID
                     IGGVPVNYVDVGEQGDRRPIVFVHGLSGQWQNWLENIPRFAEQRRVIALDLPGFGRTP
                     MPKEEITIDYYGRVVAELCDRLDLAPAVLVGNSMGGYVAAEAAIDAPESVERLMLVSA
                     AGISQSELPFGSVMRAGKILALAMRASTAQRRRQMARPALRHWILSMVVRHPTRLRPD
                     IAFEGLVKGSGKPGFVDALGACIDYDFRDRLPEVACPTLVVWGRNDAVIPVADADTYV
                     ELIPGSRKLIFEDTGHVAMVERPIAFNDELQRFLDYDVSPDELDEPRSPAAIAGDRTA
                     "
     gene            complement(18504..20177)
                     /gene="ilvD"
                     /locus_tag="VHF88_08265"
     CDS             complement(18504..20177)
                     /gene="ilvD"
                     /locus_tag="VHF88_08265"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX2161799.1"
                     /translation="MSRDIRERSRAQLDGPDRAAARAYLKGIGYDDEALRRPIIGIAN
                     TWTEVMPCNFHLRRLAERVKEGVRAAGGTPMEFNTIAVSDGITMGTEGMKTSLVSREV
                     IADSIELVARGHLFDGVIALSGCDKTIPGCVMALARLDLPSIMLYGGSIAPGNFRGER
                     VTIQEVFEAVGAHAAGKISDADLHELESVASPGAGACGGQFTANTMALAFETLGISPM
                     GSSMVPAEEGKKGQVAEDVGRLIMDVLERDLRPSRIITPQSLENAIAAVAMTGGSTNA
                     VLHLLAVANEAGVPLEIDDFDRIAARTPLLADLKPFGTYVAPDLWRAGGMPLVAKRLD
                     EAGLLHREALNVTGLTIGAEVDAAEEAEDQPVVRPLSDPLAPSGGFAILRGNLAPDGC
                     VVKLAGHGRREHRGRARVFECEEDAFAAVKASSIEAGDVVVIRNEGPAGGPGMREMLA
                     VTAAIQGEGLGDSVALLTDGRFSGATHGFMAGHVAPEAPRGGPIAAVRDGDTVIFDVE
                     RRELNVDLPADEIARRVAGYEPPPPKYTTGVLGKYARHVGSAAQGALTT"
     gene            complement(20174..20767)
                     /locus_tag="VHF88_08270"
     CDS             complement(20174..20767)
                     /locus_tag="VHF88_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161800.1"
                     /translation="MLALLTTALVVTALAVFVLGSRLVARLAARRRGAGAGAAPPAFD
                     PGRERRAEQRARELLRSCVNEVEWAMYRDLGFIRLDGRSLPDAPGSDGAQAPYAYLIY
                     PHKPIVAYVPATGRLLSEYCVNFPDVSGGGRTRLPASDDVLAKWMALTSDEARVIGRA
                     NMHLVGRQHDPGHVRRDIWRLSEWERLRAGKVGARAA"
     gene            complement(20771..21004)
                     /locus_tag="VHF88_08275"
     CDS             complement(20771..21004)
                     /locus_tag="VHF88_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161801.1"
                     /translation="MKPGHGEVLLAEGDRRVRADEELLIEEFRRQLDEGMWAAVPTER
                     PGSGRREAQMVRDYGEIPPEAQRVIFFPRASGG"
     gene            complement(21029..21832)
                     /gene="thiD"
                     /locus_tag="VHF88_08280"
     CDS             complement(21029..21832)
                     /gene="thiD"
                     /locus_tag="VHF88_08280"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX2161802.1"
                     /translation="MTASTPPRLLSIAGSDSGGGAGIQADLKAFARCGAHGMTAITAI
                     TVQNTVAVSAVYPLPREAIVGQVRAVVDDIGVDAVKVGMLGSVETIAAVAEALELVGQ
                     APVVVDPVMVAESGARLLEPDAIEALRRSILPRAAVVTPNLPEARVLAGADEQTPPGD
                     LARAIHALGPAAVVVTGGHAEPIADVFFDGGEPVAIPGERHPDGAAHGSGCTHSSALA
                     ARLALGDTPLDAARAARAVAAEAVRDGLRGIGAGAGPVDVFGLASPRAH"
     gene            complement(21846..22976)
                     /gene="dprA"
                     /locus_tag="VHF88_08285"
     CDS             complement(21846..22976)
                     /gene="dprA"
                     /locus_tag="VHF88_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX2161803.1"
                     /translation="MSGACDACLRRGYLIGLLSARIAGLTGRPGAPSELFALEDGALC
                     EAVGGAGRQEGLAFLGRFEPRRARADLRHAGVAAICRHHEGYPKALEDLSDQPAVLFC
                     RGRLELLASIDDQPAVTVVGTRDATPYAIDVAHDIGRGLGVAGITTISGMALGVDGAA
                     HRGALGVRGATIAVLGGGPDVPYPRSHRDLHRQIGESGLIVAELPPGQRPYRWSFPAR
                     NRLMAALARMTVVVEAGEASGTLITADFAQALGRTLGAVPGRVSTGAARGSNRLLREG
                     AAVVRDASDVLDELFGAGAATSLPIEIAEPEIDPLERRLLDAVESNLDIEAMSTFAGL
                     PVSETRAALGQLETRGLVCRDGLFGWRRTRRSPGREVSGPRA"
     gene            complement(22973..24490)
                     /locus_tag="VHF88_08290"
     CDS             complement(22973..24490)
                     /locus_tag="VHF88_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX2161804.1"
                     /translation="MLASVPTFAIEGVDAREVTVEVDARRGLPAFTVVGLPDAAVREA
                     RERVRSALLNSELEFPLQRLTVNLAPAWVRKAGASFDLAIAVGVLAASGQVPASALDG
                     CAVSGELSLGGSLRPVRGALPIALGCRAAGIRRLVLAPEIAPEASLADDLEVCAVPDL
                     RRLVELLHDRWAPEPPAARRRAPAQPADQLPDLADVRGQSDARRALEIAAAGGHSLLM
                     VGPPGAGKTMLARRLAGILPPPTIEEALDITRVQSVTGGADGLASERPFRAPHHTISA
                     QGLVGGGTRPRPGEVTLAHRGVLFLDELAEFARPALEALRQPLERGSVDIVRGQVALR
                     FPARVLLVAAANPCPCGVGGQRCECGVLTRERYRARLSGPLIDRIDLVCQLAPVPALA
                     LASDAAGESSTTVRTRVVAARERQAARSDATGAASNAELASGATRSLVPLDGPAARPL
                     AAALDAGVLTGRGHDRVLRVARTIADLAGAEQVGRDHVAEALGYRAVAPGRTAAA"
     gene            24629..25015
                     /locus_tag="VHF88_08295"
     CDS             24629..25015
                     /locus_tag="VHF88_08295"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX2161805.1"
                     /translation="MAGGDTRIQLGAHGEELACAHLRRAGYTLIERNGRCGRIGELDV
                     VARHGRCLVFCEVKTRLAGGVAGPAGPLDAIGPAKQRRIRRLAAEWLRTRPQSGGAPW
                     IRFDAIGVLVDRQGRLLALDHVEDAF"
     gene            complement(25012..>25630)
                     /locus_tag="VHF88_08300"
     CDS             complement(25012..>25630)
                     /locus_tag="VHF88_08300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX2161806.1"
                     /translation="FNHDRRLGARFVAGADEAGRGCLAGPLVAAAVLFDLERLTLGDR
                     RALARLNDSKQHSEEAREELYPLVMRACARTAVVSRCVRGIDSRGLHVTNLDALRSAL
                     RRVAVPGTVCLVDGFRVPPFEHEQRAVVDGDARSAAIAAASVLAKVSRDRYMRRVAER
                     HPEWDFAAHVGYSTPDHRAAIAAHGVSPIHRMSFNSTAYQQLTLG"
BASE COUNT         3421 a         9195 c         9326 g         3688 t
ORIGIN      
        1 tcggcgtccc gggcagcgcc gaccggaccg agctgatcga ccggatcgcc gcgatcgcgc
       61 acgactgcgc ggcgcgcgcg atcggcgagc cggacgcggt cgtggtctgc gcgggcgtcg
      121 aggcctcgtc gtgggacgac ctggcgatcg gcctgcggtc gaccgacgtc gtgctcgccg
      181 gggcggtcga catgccggcg cgtccctggc acgacgtggc cgcgcccgac ctcgagcgcg
      241 tgctgtggtc gctgcgcgac cggccgcagc tgcgcgactt cgccgcgacg cggctcgcgc
      301 ccttggtcga gcacgacgcc cgccgctcga cgaagctgct cgacacgctg cgcgtcctct
      361 gcgcggccgg cggccgcagg gcggaggcgg cgcgcgcgct cggcatcgag cgccagtcgc
      421 tctaccaccg gctcgggcgg atcgagcagc tgctcgacgt cgatctgtcg gccggcggga
      481 cgctgttcga gctggagctg gccctgcgcg tgcgggacat cggctccgct tgagccgccg
      541 gcgcccgctc gtcggctacg cggcggcgct gacggccgtc acgctctggt cgctcaacgc
      601 gagcgtgtcg cgcacgctgc tcgacggcgg gctgagcgcg agccggctgg ccgaactgcg
      661 cacggccggg acgttcgcgc tgctgctggt cgcgatcgcg ctcgtgcgcc cccggctgct
      721 gcgcgtgccg atcggcgaga tccgaccgct cgtcgcgctc ggcgtcttcg ggatcgggtt
      781 gctgtacgtc agctactcgc tggccgtcga gcggatcaag atcggcgtgg cactgacgat
      841 cgagtacctc gcgccggcgc tgatcctgat ctggctcgcc ctagtacggc ggcgcaggct
      901 gcccgccgag ctggtcgcgg cgatcgtcct cgcgctcgtc ggctgcgcgg cgatggtcgg
      961 ctcgtacggg tcactcgacg gcgtcggcgt agccgctgcc ctggccgccg cgttcggcta
     1021 cgcgttctac ctgctgaccg ccgaacggct gagcgggcgc taccaccccg cgaccacgct
     1081 cgtctgggcg ttcggcgcgg gcacgctggt cctgttcgcg gtccagccgc cgcactcgtt
     1141 cccgttcgag cgcctcgagg ggtccgccga cgtcgcgctc gccgtcgagg tgatcgtcgt
     1201 tggcacgctg ctgcccttcg ccctgatgct gctcgcggtc cgccacgtca cggcgatccg
     1261 gattgccgtc gtgggggcgt ccgagccggc cctgtcagcc ctcttcgcct atctgatcca
     1321 cgacgaacgg ctctctgtcg tccagatcct cggcggcctg ctgctgctcg gagccgtcgt
     1381 ctgggtccag gtccagcgtc ccaacctgcc cgccgagctg gcggccgcgg gcgccgagcc
     1441 gcgccggcag cccgcgggca gggcctgatc cggcgcgcga ccggccgggc cggtgcgccc
     1501 cgcgacgggg cctacgcctc ggcgtcgtcg cgcctggcgg caaccttctg ctcgagctgg
     1561 ctcatcacgt cggggtcgcg caacgtcgtt acgtcaccga gctcgcggcc ctcggcgatg
     1621 tcgcgcagca ggcggcgcat gatcttgccg gagcgggtct tgggcaggtc gtcggcccag
     1681 acgatgcgct tggggcgggc gagcttgccg atccgctgtg cgacgtgctc gcgcagctcg
     1741 gcgatccggt cgtcgtcgcc gtcctggtcg ccctcgaggg tgacgaacgc gcagatcgac
     1801 tgaccggtgt cctcgtcctg ctggccgatc accgcggcct cggcgacgcg cgggtgggag
     1861 acgagcgccg actcgacctc ggccgtcgag aggcgatgac cggacacatt gatcacgtcg
     1921 tcgacgcggc cgatcaccca gaagaagccg tcctcgtcct tgcgcgcggc gtcgccgacg
     1981 aagtaggtgg tcgggccgaa cttcgagaag taggtctcct cgaagcgctc ggggtccttg
     2041 tagagcgttc gcagcatgcc cggccagggg cggcgcagga cgagcagccc ctgaccgtcg
     2101 gcctcgtagc cgtcctgatc gacgacggcg gcgtcgatgc ccggcagggg acgggtcgcc
     2161 gagccgggct tggccgcggt gatcccgggg atcggactga tcagcacgtg gccggtctcg
     2221 gtctgccacc aggtgtcgat gatcgggcag cgctctccgc cgatgacgac gtggtaccag
     2281 acccacgcct tcgggttgat cggctcgccg accgtcccga gcaggcgcag cttcgagagg
     2341 tcgtggcggg ccgggtgctc ggcgccccac ttcatgcagg cgcggatcgc cgtcggggcg
     2401 gtgtagaaga gcgttacgcc gtagcgcgcg cagatctccc accagacgtc cttgtcgggg
     2461 tagtcgggcg cgccctcgta catcaccgac gtcgcgccgt tgagcagcgg cccgtagacg
     2521 atgtaggagt ggccggtgac ccagccgacg tcggccgagc accagtagac gtcctcctcc
     2581 ggcttgaggt cgaaaaggtg cttgtgcgtc caggcgacgc cggtcaggta gccgccggtc
     2641 gtatgcagga tccccttcgg cttcgcggtc gagccagacg tgtagagcac gtagagcgga
     2701 tgctcggcgt cgagcggcgt cgccgggcag tccggggcgg cctccgcgag tgcctcgtcg
     2761 aaccagacgt cgcggccgcc gcgcatgtcg acgtcgttgc cggtgttgcg cacgacgacg
     2821 accgtctcga tcgccggcag gctgtcgagg aaggcgtcga cggcgggctt gaccggcgcg
     2881 gtcttgccct tgcgccgggc gccgtcggcg gtgatcagcg ccttcgcctc ggaaaactcc
     2941 atccgctcgc gcaccgactc cggcgcgaag ccgccgaaga cgacgttgtg cggcgccccg
     3001 atccgcgcgc aggccagcat cgccacgacg acctcgggga ccatcggcag gtagatcccg
     3061 acgacgtcac cgggcccgac gccacggtcc ttgagcgcat tcgccagccg ctgggtgtcg
     3121 cgcagcagct cggcataggt gacgtcgcgc tcctccccct cctccccgcg ccagtggaag
     3181 gcgacacggt cgccgagccc cgcctcgacg tggcggtcga ggcagttgta ggacgcgttc
     3241 agcttgccgc cctcgaacca cctgtagaac ggcgcctcgg actcgtcgag cacggcgtcc
     3301 cagcgctcga accagtgcag gtcctcggcc tgccgcaccc accaccccac cgggtcgcgc
     3361 tcggcctcct cgtagatcga cgggtcggtc agcagcgccc gctcgacgaa ctccgccggc
     3421 ggctcgaagc tctcccggtc gagcagctcg gcgatccgcc gctccagctc agtcgtccca
     3481 gcgctcgcgt ccatcggctc ctcctggtgg ctctgcgctg ttgtacccga tacggcgaga
     3541 ggcgacgacc ggaatcgaac cggtgtaaac ggctttgcag gccgctgcgt aaccactccg
     3601 ccacgtcgcc gggtggaggc gagaagccta ccgccgcccg caaggggcgg cggggcctat
     3661 gtcatccctg tacggaggct aggcgccgag gcgctgctcg aggtcgtcga gctgggcgcg
     3721 gatgccggcg gggagctggt cgccgaactt ggcgaggtgc tccttgacct gcggcagctg
     3781 ggccttcagg gcctcggggt cgacgcgcaa cagctcgtcg aggtcggcgg cgtcgatgtc
     3841 gaggccgtcg gtgttgaggt cggaggcgtc cggaacgagg ccgaacggcg tctccgcagc
     3901 cttgccctcg ccgttgcagc ggcggaagac ccactcgagg acgcggctgt tctcgccgaa
     3961 gccgggccag aggaagcggc cgtcgtcgtc cttgcggaac cagttgacgt agaacatctt
     4021 cggcaggtcg gcgccctcgc gctcgccgat cttcagccag tggcccatgt agtcggccat
     4081 gttgtagccg cagaacggca gcatcgcgaa cgggtcgaag cgcagctggc cgacctggcc
     4141 ggcggcggcg gcggtcgtct ccgacgacat cgtcgcgccg aggaagacgc cgtgctccca
     4201 gtcgcgcgcc tcggtgacca gcggcaccac ggtcgagcgg cggccgccga acagcatcgc
     4261 gtcgatcggg acgccggcgg ggtcctccca ctcgggcgcg atcgccgggt cctgggcggc
     4321 cggcaccgcg aagcgggcgt tggggtgggc ggccggcttg tccgactccg gcgtccagtc
     4381 gttgccgtgc cagtcgatca ggtgggcggg cgcctcgtcg gtcatcccct cccaccagat
     4441 gtcgccgtcg tcggtcttgg cgcagttggt gaagacggtg ttgcgctcga tcgtcgccat
     4501 cgcgttgggg ttggtcttct cacccgtgcc gggcgcgacg ccgaagaagc cggcctccgg
     4561 gttgatcgcg tagagacggc cgtcggcacc gaacttcatc caggcgatgt cgtcgccgac
     4621 ggtctcgacg gtccagccct cgagcgtcgg gatcagcatc gcgaggttgg tcttgccgca
     4681 ggccgacggg aaggccgccg tcacgtactt gctctcgccc tgcggagagg tcagcttgag
     4741 gatcagcatg tgctcggcca tccagccctc gtcgcgcgcc atcaccgagg cgatccgcag
     4801 cgcgaagcac ttcttgccga gcagcgcgtt gccgccgtag cccgagccga acgaccagac
     4861 ctcgcgcgtc tcggggtagt ggacgatgta cttgttgtcg gcgttgcacg gccacggcac
     4921 gtcctcgacg ccgtcggtca gcggcatgcc gaccgagtgg acgcagggga cgaactcgcc
     4981 gtcggcgccg agcacgtcga gcgcgccctg gcccatgcgg gtcatgatcc gcatcgagac
     5041 cgcgacgtag ggcgagtcgg tcagctcgat gccgatgtga gcgatcggcg agccgagcgg
     5101 gcccatcgag aacggcacga cgtacatcgt gcggccctcc atgcagccgt cgaagatgtc
     5161 gtcgagtgtc tcgcgcatct cggccggcgc gcgccagttg ttggtcggtc cggcgtcggc
     5221 ctcgtcctcg gagcagatga aggtgcggtc ctcgacgcgc gcgacgtcgc cagggtcgga
     5281 cagagcgagg taggagttcg ggcgcttggc gtcggagagg cgctcgaagg tgccggcgtc
     5341 gacgagcgtc tggcagagcc ggtcgtactc ctcggcgctg ccgtcgcacc agtggatcga
     5401 cgcgggcttg gtgagggcgg cgatctcctc cacccaggcg acgagcttct tgttctgggt
     5461 gggcggcgcg gtcatctcgc tcatggggct cacggcgggg gtctgctcct cggtggctga
     5521 catgtcgggg gcgctgcggg gccagggacg gtaccggacc gtcaggcggc cgggcggccg
     5581 ccgaaccgct acccgcgtgc ggtttggcgc aaacggcgct gggctatcgt cggacgcctc
     5641 gcaagccagc aacaaggagg tagcgatgcc gacgcggacc ggaagcggcg agtggcaggg
     5701 cgatctgaag ggtggcgcag ggcagctcga ggtcggcgac ggggtcttca gcgggcccta
     5761 ctcggccaag tcgcgcttcg aggagggcga cggcaccaac cccgaggagc tgatcgccgc
     5821 ggcccacgcc gcctgcttct cgatggcgct cagcaacatc ctctccagcg acgggcacat
     5881 ccccgactcg gtgcgcacca ccgccgagat cgagctccgt tcccccgaca ccggcccccc
     5941 gatccgcaag atcacgctgc gcaccgaggg acgcgtgccc ggcatcgacg ccgacgcctt
     6001 tagcgagtac gccgagcggg ccaaggacga gtgccccgtg tcggtcgcgc tggccggagt
     6061 gcccgagatg gaggtcgagg ccaccttcga ggcctgaacg gcctcccgcc ccgtgacggg
     6121 ccggcgcggc gatatcgtta tcgtgtcgca gccatgcggc tgtagctcag ctggctagag
     6181 cgtctgcttg ccatgcagaa ggtcgtgggt tcgaatccca tcagccgctt gcctgcaaac
     6241 ccgctccgag agcgggtttg ctgcgtttcg ggaacattgt ggcgcccgag ggtgcccgcg
     6301 ccactgagct gcagcggatc aaagggcagc ccgcgaagct tcgtcgacct tcccagctac
     6361 gtgacgatct catcccagcc ctccagttct aatcgtcgcc attcgtcaag ggcacgagca
     6421 cggttggcgc cgcgatgcca gcgcttgccg gaatggcaga gcccacaccg aggccgaccg
     6481 caggcgagat aagcctggcg gtcctcgagc cggccgtcgg gaagaacgcg ctcgggcaat
     6541 ttcgggccgc gatctagctg gagccggcgg taggcgttct gacgtttgcg gacaatccgg
     6601 tcacggtgga accggcgacg gtgcgcggtg cgcatgttgg gcctccaaag tcggccccgc
     6661 cccgacgcct ctcgccgggg ctagggactc tgtcggcctc gcgttcgcat ggaacccgat
     6721 caggctgcgg cgagcccagg agcaagctcg gccgcgctgg cctcgacttt ctcggggatg
     6781 gtcacaacca ggcgacgatc gcggtcgagc agccagcggg cgttgacgat ggtgagtccc
     6841 acgatctggt ctgtgcctgg agcgaagcgc agtacatgcc cctcgggcgt ctcctcgccc
     6901 tcggcgggct gcggcgcgcc tcggtggagg tagaggacgt cgccgtccga gtcgtagtcg
     6961 gcgtggtcga agaccaaggg gccgatgtgg atgttcacga cttccatcgc ttcgggtcct
     7021 ttcgagtcgc gagcgcggtg gtgacccggc ccggctcctg ctcatagcct atctgcaacc
     7081 tgcaagaccc tcggactcgg gctaatgtgg ccggcacgat gaggcccgcc ggcgccgtgc
     7141 tgaagcggac gctcctgtcg ttctacgacg accagatgac ccaccatgcg gcggcgctga
     7201 cgtactactc gctgatgtcg ctgttcccgg cgctgttgct cgggctgtcg ctgctcggac
     7261 tgctgggcca gtaccccgag acctacgacg cggtcatggg ctacctgcgc gaggtgtcgc
     7321 cggagtcggt gatcgagccg ctggacgact cgctgcgctc ggcgctgcag agcaagggga
     7381 cggcggcggc gagcctgacg atcagcgtgg tggtgacgct ctacgggacc acgggcgcgc
     7441 tcgaggcggc gcggcgggca ctgaacgtgg tcttcgaggt cgacggcggg cgcagcttcc
     7501 tgcagcgcaa ggcgctcgac gtggcgtcga ccttcgtgct gatggcgctg atactggcga
     7561 gcgtcgtgat ggccttcgtc ggcggcgcgt tcgccgagga cctgctcggc ttcgtcggcc
     7621 tcggtccgac ggccgcggcg atctggagca tcgcccgctg gccgggagcg ctgctgatcg
     7681 cgatgctcgt gttcgcgctc gtctactacg tcacccccga cgtcaaacag cgggcgttcc
     7741 gctggatcac gccgggagcg gtcgtcggcg tcgcggtctg gctgatcgcc tcgctcggcc
     7801 tgtcggccta tctgtcctac atcgcggacg tcgccgccgt ctacggggcc ttcaccggag
     7861 cgatcgtgct cgtcgggtgg ctctggctga ccaacgtcgc gatgctgctc ggcgccgagc
     7921 tcaacgccga gatcgagcgc cagaaggagc tcgccgaggg cgtccccgag cgcgagacgc
     7981 tcaacctccc cgcgcgcggc tgacgcctgc gccgggcgcg cgtccgagtc gtcgagccag
     8041 gcagcgctac ttgtcgtcgc ggttgagcag gcccttgacc ttgtcggtcg ccttgtcgat
     8101 gccctccttg accttgccgg cgccctcgtc ggccttgccc tcgcgcttga ggtcgtcgtc
     8161 gccggtcagg tcgccagcgc cctgcttgag gcggcccttc gcctcgtcga tgttgctcgg
     8221 tccgtcggcc atgtcgtcct ccttcatcgg gtcgtggagt cagggttacc cggttcgccc
     8281 gtcgccaagc cgggtaaccg tcggccatgc gacccgagga gacgacgaca tgaccgacga
     8341 tcgagagcga gacccgctgg tcgacgagca ggccgaggcc gcggcccgcg aggcggcgga
     8401 gatcggcgga cccgacccga cgccaggagt cgaccccgcg gagcggccgc gcgtcgaggg
     8461 cggcgggggc tatgcggagg gcttcgagca ggccgaggag cagctccagc gcaacgcctc
     8521 gcacgaggac cgcggcgccg acccgatcgg cgacgccggg accgacgagc gcgccgacgt
     8581 gggcgcggag tacggcgagg ccgaccacgc cggagcgcag ggcgaggacc ccggctcgca
     8641 gagcgacgcc tccgagccgg aggacgtggg ctcctaacga ggcgccggac cgaacggcac
     8701 cagcgccgcc tattgtcagc cgcatggcct actcgaccgc cgagggacga caggagctgc
     8761 tcgacgggct ggcgggcgcc atcgacgagc tcggctacgc gctggcgtcg ctcggcgccg
     8821 cctacgagca gctcgaccag gcgacggccg acaagctcga ggaggagctg ttcggaccgg
     8881 tccagcgcgc ctacgggcgg gcaaagcgga cctatggggc gttcgcccag cgggccggcc
     8941 tcgagcccgc gcgcttcgac atgcagtcgg ccggcctgcc ctcgacgggc gccaaggggt
     9001 tcgtcgagca tgcggtcgag ggcatcagct cggcgggcgg caggctggcg acgctgcagg
     9061 actcgcccgt cctgctcgag gtcggcgacg ccgagctgcg tcagggactg accgagatcc
     9121 gcgagtcgat cgcggacctc ccccacgtcg cgcgcgagct gctgcggcgc ctaggccggt
     9181 agagccgcgc tccggccgat cagctgcagc ttcgccgccc gcggcaggcg cttgatgcgc
     9241 gcctcctcgc gcagcgccgc cgagcggtcg gccgcctcga acaccgcggc gagctccacc
     9301 ggccggcggg tgcgcgtgta gcggctggcg cggcccgcct cgtgctcgcc cagccgccgc
     9361 cgcacgtcgg tcgtccagcc gcagtagagg ctgtcgtcgg cgcagcgcag caggtagacg
     9421 taggccacct ccccagcttc gccgtccggt cgcggttcgc ctgccacgcg gatatgttgg
     9481 cgcggtgccg cgcttcgtcg acctctctgc gccgatccgc caggaccccg ccgagctgcc
     9541 cgagctgctg cggaccgaca tcgcctacac cgaccacgcc ggcggggcgc gcgacatcga
     9601 ggcgctgacc ggcgtgacgc cggacctgct gcgcgacggc gagggctgga ccgtcgagac
     9661 gttcaccaac ttcggcaccc acagctcgac ccacgtcgac gcgccgtggc actacaactc
     9721 gacgatcgac ggcaggcgcg cgcagtcgat cgacgagctg ccgctcgact ggttcttccg
     9781 ccccggcgtc gtgctcgaca tgaccgccaa ggccgacggc gacccggtca ccgtcggcga
     9841 ggtcgaggcc gagctcgagc gcatcggcca cgagctcgcc gagcgcgaca tcgtcctcgt
     9901 ccgcaccggc cgcgacgcct tctacgacga tcccggctac atggcgctcg gaccgggcgt
     9961 caccgtcgag gcgacgcact ggctctacga gcgcggcgtg cgcgtgatgg gcatcgacgc
    10021 ctggggctgg gacgcgccgc tctggatgca ggccgaggag gcgaagcggc gcggcgagcg
    10081 ggggatcttc tgggcggccc accaggccgg cctcgcctac tcccagatcg agcgcctgtt
    10141 caacctcggc gagctgccgc cgacgggctt caccatcgcc tgcttcccgc tgaaggtggt
    10201 cggcggcagc gccgggccga cgcgcgcggt cgcgatcctc gacgaataga cggcgccggc
    10261 ttaggcgacg acgcgcaggt ggcggaacgg ccggccgcgc agctcgcgca cgaccgcgtc
    10321 gacgagccgc tcctcgtcgt ggacctggcc gtcggtgacc gcgtcgatca ggctgcgctt
    10381 gcgctgcagg agctcggcca tcgtctcgtc gatcgtgttc ggcgcgagca ggtaccacgc
    10441 cgtgacagcg ctctcctggc cgatgcgatg caggcggtcc tcggcctggt cgtggcgggc
    10501 cggcgtccag tcgagctcga ggaaggcgac gttggaggcg cgcgtgagcg tgatgccctg
    10561 ggaggccgcg cgcatcgagc agacgatcag ctgcggccca tcctcgcgct ggaacgcgtc
    10621 gacggcgcgc tggcgggcct gcgtcgtatc ggcgccgagg atgtgcgccg cgccggggaa
    10681 gcgctccatg atcgccttct ggatcgcgat gtgctcggcg aagacgacca gcggctcgcc
    10741 cgactcgagg aagtcggcga tccaggcgag cgccgtcggc agcttgccgg ccgccgcgag
    10801 ctggcgcagg ttgttcagcc ggaccagctg ctcggcccgg agcgcggcgg cgatcttcgc
    10861 gtcgagcgtg ccgaggtcga gtggcagcgt ctgcagccag gcgatcacgt cctcctcggc
    10921 gagccggtac tcgtgctcgt tggagagcag gaccggaacg gtgtcctggc gcttggaggg
    10981 cagctggggc agcacctgct gcttggtgcg gcggacgtag cagaccgcgc gcaggttcca
    11041 gtgcagccgg tcgtcgctgc ccgcggtgcg gaagcggcgc gagaggcgcg cgccgctgcc
    11101 gaactcgcgc agccgtccga gcacgcgcag ttgggcgatc agctcgtcgg ggcggttgag
    11161 gatcggggta ccggtcagcg cgagccgcag ggagtcgtcg ggcagcccgc cggcgagctc
    11221 gagcgccgcc ttggtgcgac ctgcgcgcgg gttcttgacg tagtgcgact cgtcgaggat
    11281 caaggcccgc ggaccgctgt cgagcaggcg cgacagatgg gcttcgagga tgtcgtagtt
    11341 gagcacgacg atctccgcct gctcggcggc ctcgtcccag gtcgcgtcgg tgcgtccgtc
    11401 gaggacggcg acgcgccgct cgggcagcca gatgcccgcc tcgcgctccc aggtcagctt
    11461 catgctcgcc gggcagacga cgagcgccgg gaaggcgtcg tcggcctcca gcgcggcgag
    11521 cgcctggacc gtcttgccga gcccctgctc gtcggcgatg aacgtctgtc ggcgggcaag
    11581 cgcatagcgg acgccggcgc gctggaaggg gtgcagctcg ccaccgaggg tcacgccgtc
    11641 gagttcggcg tcgtcggcgt aggaaagcgc gaccgtcgcc gacgcgcggt cgtgctcctc
    11701 gatcagctcg tcgagcaggt cgcgcgcgcg gtcgtcgagg ccgacgccag gatggttggc
    11761 gacgaacccg gcgaggtcac gcgcgagcgc gggatcggcc ggcaccgcga ccccccaggc
    11821 ggcgtcgcgg ttgaacacgc gcgcgctgcg cgggacgggc ttcgcctccg gcagctgctt
    11881 gtagtcgcgc gccacggccg ggtcccagac ggtgtagagc gtgagctcgg gctcaccgtc
    11941 ctcgccgatc tcgacgccga gctcggccgg cggcggcgtg cgccctgccg ccaggtcgcg
    12001 gatgcaggcc tttgcgaggt cgtcgatccg cgcgccgtcg agctgggcga gccgggcggc
    12061 cgcctcggca tcgaacggga agacgagccg atcctcgccg gtcgcgatcc cgccctcgag
    12121 cccggtcgcc ggcagctccc ccgacaccgt gcgcacgacg aaggcgcccg cgccggagtg
    12181 gatcacgacc gatacgagtg cacgccagcg ctccgagtcg gtcagccagg cgatcacgtc
    12241 gggctcgacg agcagctccg ggaaccgctc gagcagctcg ccgaccggct gcgccgcgcc
    12301 cggctcggcc ggcacccgcc agtgcttgcg ccgccagtcg aaccagcgcc cggggagctg
    12361 cttgaccgcc tcgttgagct gctcgtcgaa cgggaaggcg aagaccagct cctgcttcga
    12421 gcgctcctcg gccagcatcc agacccttgc ctgcgggtgc gcagcctggc tgtcggggcg
    12481 agcgtgcatc aacccaacga gactagtgag ccgcgcggcg aagcgagcag cgagcgttag
    12541 cgggcctgca gctcggcgtc gagcgccgcg ttcacgagcg catcggcgcg cgtgttggac
    12601 tcgcgccgca cgctgcgcac ctgccagctg tcgaaggcgc gcagcgccgc catcgcctcg
    12661 aggtagagcg gcttgaggcc ggcgtgcttg acgcggtagg cgcccgagat ttgcttggcg
    12721 acgagctccg agtcgttgat cacctcgacc tcgctcgcgc cgagctcgcg cgccagctcg
    12781 aggccgagca gcagcgcgcg gtactcggcg acgttgttgg tcgtctcgcc gatgtaggtg
    12841 ccgcgctccg cgagcacgcc gccgtccggg tcggtggcga cagcgcccac ggcagccgga
    12901 ccagggttac cgcgcgcgcc gccgtcgacg ttgacgacga ccttgcgcgg cgcggccgaa
    12961 gcgcccggct ctgccgggtc cgcccggttc gtcatccgcc gggcggcgcg tcgatcggcg
    13021 ggaagctgcc cttcggccgc acgcggcggc ggtcggtcat cgggtcgtgg ctgccgggcg
    13081 tgcctccttg ggcgcggcgg tcgacgatca cctcgacgtt cggctcgtcg ctgtagtgct
    13141 cgacgagctt gtcgtacagc tcgtcctcca gcgctcgtgg taccacgcag tagatcaccg
    13201 gacggctcct ccttccatga ccagccggcc ctcctcgtcg agaccggcgt tccagcgccg
    13261 cagcgcctcg tcgatgtcgt tcagctcgtc gcccttctcc tgcagggcga ccggctcggc
    13321 cgtgtcgccg aggtcgaccc ggtagcggta ccaggacagc tcccaggcga cgaccacggt
    13381 caccgcgctc ggtgccgcga cgtcgggcac cgcggtggcc cagggaaagc cgagcgtgcg
    13441 cgtcagcccg gcgaccgttc gctgatgctc ggagccgttg aacagctcca gcgcccgctc
    13501 gaccttgacc tccgcggtgg tcggcaccgc ccgcacgtgg cgcggatcct gcggaggctc
    13561 gcggcgccgg ggcggcgtcg cgggctcctc ggcggccgtg tcgacggctc gcgcctcgac
    13621 ggcggcggag tcgtgggcgt cagccgccga ggcgggctcg gcataggcgc cggccgtccc
    13681 cggctcgacg cgcgggccgt cgacggcccc ctcgcggcgc cggcgcaggc ggccgaagat
    13741 ctgacgccgc tcctgcggcc gtcccgcgcg ggtcgggatc tggtcggcgg aggactcgcg
    13801 gatccagccg gcgtgctcgg ctcgcacgaa gcagagctcg cagacgaggt tgcggtggcc
    13861 gccgggcgcg agaaagtgct cgatccgctc gcccttgagg atcgtccggc cgcagacgtc
    13921 gcaggagacg gggtcgtgga acggacgcag ctccttcttc actggctcga tgctactgcc
    13981 gcgtcgaccg tcggatgcac cggcgcgacg gcgcgcacgt cggtcagctc gaggacgcgc
    14041 cgcagcgggg agtcgcccgg catcgcgatg cgcaggtcct ggcggtggcg gccgagccgg
    14101 cgcgcgagct cgaacagcag ctcgatcgcc gccgagtcga gatagatcgt ctcgctgagg
    14161 tcgacgacca ggccgtcggc ggcgccgggg atcgagcgca tcagctcgtc gcgcacgtag
    14221 ctgcagttgg tcatgtcgag ctcgccgccg acgcgcgcga cgatccactc gccgagccgt
    14281 tcgatagcga tgtcggccgt agtcgtcaac gtccgctgct cactcgaccg cttccgtgag
    14341 ggcgtcgccc tggagcttcg acttcagtcg caggacagcc ttggtgtgga gctgcgagac
    14401 gcgcgactcc gtcacgccga gcacctcgcc gatctcgcgc agcgtcaggt tctcgtagta
    14461 gtagagggcg atgaccagct tctcccgctc gggcagccgc gcgatcgcgt cggcgaggct
    14521 gtccttcatg tcggtcaggt cgagctcgcg cgccgggtcg gctgcgtgcg gatcggggat
    14581 cgtgtcgagc agcgacacct ggtcgccgga ggagtccgac accgtccaca gctcgtcgag
    14641 cgctaccacc gaggagttcg cgatcttgac cagcgactcc tggaactcca cctgggtgac
    14701 gcccatcgcg tcggacatct cggcgtcggt cggcgcgcgc tggagcttgt tctcgagctt
    14761 cgcgttggtc tgctcgatct cgcgcgcctt cgcgcgcacg gagcgcggca cccagtccag
    14821 cgagcgcagc tcgtcgatga tcgagccctt gatccgcgtc accgcgaacg tctcgaagcg
    14881 gatctcgcgc tcgaggtcga agcgctcgat cgccgagatc agcccgagca ggccgtagga
    14941 gatcaggtcg gcctcctcga cgtgggcagg cagtccgctc gacatgcggc cggcgacgta
    15001 cttgacgagc ggcgagtacg cgaggacgag gcgctcgcgc gcctgctggt cgccgtcctg
    15061 cttgtaacgg ctccaaaggt ccttcagctc gatcgccttg aggttcgtct ccacctcgtc
    15121 cagtcaccct tcacgcgcgc ggatgggcct gcgcatatgc ctttctgagc cgcttcgact
    15181 ctacccgagt gtaggtttgg gtcgtgctca gggagctgtg tccgagcagc tcctggatcg
    15241 cgcggagatc ggcgccgccg tcgagcaggt gggtcgcgaa ggagtgccgc agggcatgcg
    15301 gcgaggccgc ggcctgcagt ccggcatggc gcagcgcgac gcgcaggcgg cgcctgacgt
    15361 cggttgtggc cagccgtcgc ccggtgcgcg agaggaacag cgcggggtcg cggcggccgt
    15421 ccccggcagc cgccagctcg ggccggccgc gctccaggta ggcgtcgacc gcgcgccagg
    15481 cgagctctcc cgccgggacg atccgcgtct tggcgccctt gccctcgacg cgcagctgct
    15541 cgccgtcgcg gtcgaggctg tcgacgtcga ggtcgaccag ctcggccgcg cgcaggccgg
    15601 aggcgtaggc gagctcgaat agcgcgcggt cgcgcagttc gagcggcgtc gaggccggca
    15661 gcgagtcgaa cagccggtcg acctctgcac ggctcagcac gatcgggagg tcgctgtcgc
    15721 gcttcgggct ggcgaccagg tccgcggggc tcgcctcgag ctcgccgcgc tcgatcagca
    15781 cccggtagaa cgcccggaag gcggcgagct tgcgcgcgac ggtggcgcgc gagttgccgc
    15841 gctcggacag cactccagcg aagcggcgca gggcgcgcgc gtcgacgtcg cccggcgcca
    15901 gctcctgcgc gctcgcccac tcggcgagct ggccgaggtc gctgccgtag gcacggcgcg
    15961 tctgctcgcc cagcccgcgc gtccgcagcg cggtgtcgaa cgcgcgcagc gtgcgggccc
    16021 aactagggtt gggcggcgtg tcggtcttct tcgtcaacac tcaggacggg cgggtcgcga
    16081 cccgctccca gctcgtcgac gcggaactgg ccgacgacga ccacaagccc gtcgccccgt
    16141 ggcatccgat ccagggcccc ggcggcgagt cgacgctctg gtacgcggtc ctgcgcaagc
    16201 gtgagcgtgg cgtcttcatc ggcaccctct gcattcgtca ctccgagcgc caagcctctt
    16261 tggaggccgc cggatgggag gaggtcgcgg tcgacgcgat cggcgtcgct cagccgatgt
    16321 agatgggggc gcctacctcg accctgtcgt acaggtcgat cacgtcgggc accgccatcc
    16381 ggatgcagcc gtgcgaggcg gcggtcccga gcgagccgat gtcgtcggtg ccgtggatcc
    16441 cggcgccgtc gtagatgccc agccaacgtg ccttgagcgg gttctcgggg ctgccgccgg
    16501 ggaccaccgt gcccgcgagg tcgccggccc agtcgctgtt cgggacgctc caggccgggt
    16561 cgaccgcctt gttctggatc gagtagaggc cgcgcggcgt gtcgtagccg gcggcgccga
    16621 aggcgacggt gtaggtcttg acgagcttca aatccttgta gagccgcagc tcgtagctgg
    16681 cgtggtcggc gaccagaacc gtcgggtact ccttcgcgag ctcgtcgcgg gtgacctcgg
    16741 ccttggtcgc ctttaccggc gcctcgacgg tgcggctgct cggcgacagc agcgcagagc
    16801 cgatcagccg cctcagctgt ccgcgcttga ccgcgatccc gtcctgctcc ttgacggcgt
    16861 cgagcgacgg gaagtcgagc gtcgcgtcct tgggctcgcg gtcgagcttg tcgcccaccc
    16921 gcgtcacgag cgcctgcgca gccttcggcg agtaggtggc gctgacgctg acctcgacgt
    16981 cctcctcgtc gccgcgcagg tcgcgcgtca cgcgcgcgag cacgttgtcg tcgcggctgg
    17041 cctcgatcgc gtcgtcgacc atgccgccga cgtcggcgcg gatgccggcg tcctcggcgg
    17101 agagggtgaa gcgagtcttg ccgtaggtca cggcgatcgg cttctcgatg cgagccgcga
    17161 cctggcggcg gaccttgacg cgcgcctcgt cggcgctcag gccgccgacg gcgacgccgc
    17221 cgacggtcac cccctcggcg atccggtcgc tgcgcgagga gtcataggcg tagaccgcca
    17281 ccgatccggc gatcaacagg accaccagga ccgcgacgaa cgtgaatgcc ttgctcttca
    17341 aaagtggggt tcccctccgg ccctgcgacc gaccgactca gtgtaagcag cctttcggcc
    17401 attgcacagg gcgtcccccc tcctgcaacc tgcattgcgc caggtctggt ccccgagtcc
    17461 gcgcagtggg ggttcggata cctttgtgtt tgcaatgggc cgcctgatca tcccaccgcc
    17521 acccgctcgc cgccgcgacg cggtgcgcgc cgacggcgac tacggcgtca gcgaccggcc
    17581 tgactggcgc gctgtcgact ggcccgcgca tctgcgcacg atcgacatcg gcggcgtccc
    17641 ggtcaactac gtcgacgtcg gcgagcaggg cgatcgccgt ccgatcgtct tcgtccacgg
    17701 cctcagcggc cagtggcaga actggctcga gaacatcccc cgcttcgctg agcagcggcg
    17761 cgtgatcgcc ctcgacctgc ccggcttcgg tcgcacgccg atgccgaagg aggagatcac
    17821 gatcgactac tacgggcgcg tcgtggccga gctctgcgac cggctcgacc tcgcgccggc
    17881 cgtgctcgtc ggcaactcga tgggcggcta cgtcgcggcc gaggcggcga tcgacgcgcc
    17941 cgagtcggtc gagcgactga tgctggtgtc ggccgccggg atctcgcaga gcgagcttcc
    18001 cttcggcagt gtcatgcgcg ccgggaagat cctcgcgctc gcgatgcggg cgtcgacggc
    18061 tcagcgccgg cggcagatgg cgcgtccggc gctgcgtcac tggatcctca gcatggtggt
    18121 ccgccatccg acccgtctgc gcccggacat cgcgttcgag ggcctggtca agggctcggg
    18181 caagccgggc ttcgtcgatg cgctcggcgc ctgcatcgac tacgacttcc gcgaccggtt
    18241 gcccgaggtg gcctgcccga cgctcgtggt ctggggccgc aacgacgcag tgatcccggt
    18301 cgccgacgcc gacacctacg tcgagctgat ccccggctcg cgcaagctga tcttcgagga
    18361 caccggtcac gtggcgatgg tcgagcgacc gatcgccttc aacgacgagc tccagcgctt
    18421 cctcgactac gacgtcagcc cggacgagct ggacgagcct cgctcgccgg ctgcgatcgc
    18481 cggcgaccgc acggcctagc cggtcaggtc gtcagcgcgc cctgggcggc cgagccgacg
    18541 tggcgggcgt acttgcctag cacgccggtc gtgtacttcg gcgggggcgg ctcgtagccg
    18601 gcgacgcggc gagcgatctc gtccgccggc aggtcgacgt tgagctcgcg gcgctcgaca
    18661 tcgaagatca ccgtgtcgcc gtcgcggacg gcggcgatcg gaccgccgcg cggagcctcg
    18721 ggcgcgacgt ggccggccat gaagccgtgc gtggcgccgg agaagcggcc gtcggtcagc
    18781 agcgcgaccg agtcgccgag cccctcgccc tggatcgccg cggtgaccgc gagcatctcg
    18841 cgcatccccg gaccgccggc cggcccctcg ttgcggatca cgacgacgtc gcccgcctcg
    18901 atcgagctcg ccttgaccgc ggcgaaggcg tcctcctcgc actcgaagac gcgtgccctg
    18961 ccgcggtgct cgcgccggcc gtggcccgcg agcttgacga cgcagccgtc gggagcgagg
    19021 ttgccgcgca ggatcgcgaa gccaccgctg ggggccagcg gatccgacag cggtcgcacg
    19081 accggctggt cctcggcctc ctcggccgcg tcgacctcgg cgccgatcgt caggccggtc
    19141 acgttgagcg cctcgcggtg cagcagcccc gcctcgtcga gccgcttcgc gacgagcggc
    19201 atcccaccgg cacgccagag gtcgggcgcg acgtaggttc cgaacggctt gaggtctgcg
    19261 agcagcggcg tcctggcggc gatccggtcg aagtcgtcga tctccagcgg cacgccggcc
    19321 tcgttggcga cggccagcag gtgcagcacc gcgttggtcg agccgccggt catcgccacc
    19381 gcggcgatcg cgttctcgag cgactgcggc gtgatgattc tgctcggacg caggtcacgc
    19441 tcgagcacgt ccatgatcag ccggccgacg tcctcggcga cctgcccctt cttgccctcc
    19501 tcggccggca ccatcgagct gcccatcggc gagatgccga gcgtctcgaa ggcgagcgcc
    19561 atcgtgttgg cggtgaactg gccgccgcag gcgccggcgc ccggggacgc gacggactcg
    19621 agttcgtgga ggtcggcgtc ggagattttg ccggcggcgt gggcgccgac cgcctcgaag
    19681 acctcctgga tggtcacccg ctcgccgcgg aagttgcccg gcgcgatcga gccgccgtag
    19741 agcatgatgc tcggcaggtc gaggcgggcg agcgccatca cgcagccggg gatcgtcttg
    19801 tcgcagccgg agagcgcgat cacgccgtcg aacaggtggc cgcgggcgac cagctcgatc
    19861 gagtcggcga tcacctcgcg gctgaccagc gaagtcttca tgccctcggt gcccatcgtg
    19921 atgccgtcgg agacggcgat cgtgttgaac tccatcggcg tgccgccggc tgcgcggacg
    19981 ccctccttga cccgctcggc aagccggcgc aggtggaagt tgcacggcat cacctcggtc
    20041 caggtgttgg cgatgccgat gatcgggcgc cgcagcgcct cgtcgtcgta gccgatgccc
    20101 ttgaggtagg cgcgcgcggc ggcccggtcc gggccgtcga gctgcgcccg gctgcgctcg
    20161 cggatgtccc tgctcacgcg gctctcgccc cgaccttgcc ggcgcggagg cgctcccact
    20221 ccgacagccg ccagatgtcg cgccggacgt ggccggggtc gtgctggcgg cccacgaggt
    20281 gcatgtttgc gcgcccgatc acgcgcgcct cgtcggacgt cagcgccatc cacttggcga
    20341 gcacgtcgtc ggacgccggc agccgcgtgc ggccgccgcc gctgacgtcg gggaagttga
    20401 cgcagtactc gctcagcagc cggccggtcg cggggacgta ggcgacgatc ggcttgtgcg
    20461 ggtagatcag gtaggcgtac ggtgcctggg ctccgtcgga gccgggcgcg tcgggcagcg
    20521 accggccgtc gaggcggatg aagccgaggt cgcggtacat cgcccactcg acctcgttga
    20581 cgcaggagcg caggagctcg cgggcacgct gctcggcgcg tcgctcgcga cccgggtcga
    20641 acgccggcgg ggcggcaccg gcccccgcgc cgcgacggcg agccgccagc cgggcgacca
    20701 gcctcgagcc gaggacgaac acggcgagcg ccgtcaccac cagcgcagtg gtcaggagcg
    20761 ccagcaccgc tcagcctccg gaggcccgcg ggaagaagat cacgcgctgg gcctcgggcg
    20821 ggatctcgcc gtagtcgcgc accatctgcg cctcgcggcg gccgcttccg ggccgctcgg
    20881 tgggcaccgc cgcccacatc ccctcgtcga gctggcgccg gaactcctcg atcagcagct
    20941 cctcgtccgc gcgcacacgg cggtcgccct cggccaagag gacctcaccg tgaccgggct
    21001 tcatgcgcag gaacttgatg cgtcaatcct aatgggcgcg cggcgatgcg aggccgaaga
    21061 cgtcgaccgg cccggccccg gcgccgatcc cgcgcaggcc gtcgcggacg gcctcggcgg
    21121 ccaccgcgcg cgcggcgcgc gctgcgtcga gcggcgtgtc gccgagcgcc agccgcgccg
    21181 cgagcgccga cgagtgggtg cagcctgacc cgtgggcggc accgtcgggg tggcgctcgc
    21241 cggggatcgc gaccggctcg ccgccgtcga agaagacgtc ggcgatcggc tccgcgtggc
    21301 cgccggtcac gacgaccgca gcgggcccga gcgcgtggat cgcgcgcgcc aggtcgccgg
    21361 gcggcgtctg ctcgtccgcg ccggccagca cacgtgcctc gggaaggttc ggcgtgacga
    21421 ccgccgcgcg cggcaggatc gaccggcgta gggcctcgat cgcgtccggc tcgagcaacc
    21481 gggctccgct ctcggcgacc atcaccgggt cgacgacgac cggcgcctga ccgacgagct
    21541 cgagcgcctc ggcgaccgcg gcgatcgtct cgaccgatcc gagcatgccg accttcaccg
    21601 catcgacgcc gatgtcgtcg acgacggcgc gcacctgtcc gacgatcgcc tcgcgaggga
    21661 gcgggtagac ggcgctgacc gcaaccgtgt tctggacggt gatcgcggtg atcgcggtca
    21721 tgccatgggc gccacagcgc gcgaacgcct tgaggtcggc ctggatgccg gcacccccgc
    21781 cggagtccga gccggcgatc gacagcaggc ggggcggagt cgaggcagtc ataggagcgc
    21841 gcagcttagg cgcggggccc ggacacctcg cgcccgggcg accgccgcgt gcgccgccaa
    21901 ccgaacagac catcgcggca gacgagcccg cgtgtctcga gctgccccag tgcagcgcgc
    21961 gtctcgctca ccggcagacc ggcgaacgtg gacatcgcct cgatgtcgag attcgactcg
    22021 accgcgtcga gcagccgccg ctcgagcgga tcgatctccg gctcggcgat ctcgatcggc
    22081 agcgacgttg ccgccccggc gccgaacagc tcgtcgagca cgtcggaggc gtcgcggacg
    22141 accgcggcac cctcacgcag gaggcggttg ctgccgcgcg ccgcgcctgt cgagacacgg
    22201 ccgggcaccg cgccgagcgt cctcccgagc gcctgcgcga agtcggcggt gatcagcgtc
    22261 cccgacgcct caccggcctc gacgacgacg gtcatccgcg ccaacgcggc catcagccgg
    22321 ttgcgcgccg gaaagctcca gcggtagggc cgctgccccg gcggcagctc cgcgacgatc
    22381 aggccgctct caccgatctg gcggtgaagg tcccgatggc tgcgcggata gggcacgtcg
    22441 ggcccgccgc caaggactgc gatcgtcgcc ccgcgaacac cgagcgcgcc gcggtgagcc
    22501 gccccgtcga cgccgagcgc catcccgctg atcgtcgtga tccccgcgac gcccagccca
    22561 cgcccgatgt cgtgggcgac gtcgatcgcg tacggcgtgg cgtcgcgcgt gccgacgacc
    22621 gtcacggccg gctgatcgtc gatcgacgcc agcagctcga ggcgtccgcg gcagaagagc
    22681 accgccggct ggtcgctgag gtcctcgagc gccttcgggt agccctcatg gtggcggcag
    22741 atcgcggcga caccggcgtg gcgcaggtcg gcacgggcac ggcgcggctc gaaccgcccg
    22801 aggaaggcga gcccctcctg ccgccccgcg ccgccgacgg cctcgcagag cgcgccgtcc
    22861 tcgagcgcga agagctcgga gggcgcgcct ggccggccgg tcaaccccgc gatccgagcc
    22921 gacagcagcc cgatcaaata gccgcggcgc agacaggcgt cacaggcccc gctcatgccg
    22981 ccgcggtccg ccctggcgcg acggcgcggt agcccagcgc ctccgctacg tggtcgcgcc
    23041 cgacctgctc ggcgcccgcc agatcggcga tcgtgcgagc gacccgcagc acgcggtcgt
    23101 gcccgcggcc ggtgagcacc ccggcatcga gcgccgccgc cagcggtcgc gcggcgggac
    23161 cgtcgagcgg cacgagcgac cgcgtcgcgc cggaggccag ctcggcgttg gatgccgcgc
    23221 cggtggcgtc actgcgagcc gcctggcgct cgcgcgccgc gacgacacgc gtgcgaaccg
    23281 tcgtcgaact ctccccggca gcatcggagg cgagcgccag cgcaggcacc ggcgccagct
    23341 ggcagacgag gtcgatccgg tcgatcagcg ggccgctgag ccgcgcgcgg tagcgctcgc
    23401 gggtcagcac gccgcactcg cagcgctgcc cgccgactcc gcatgggcac gggttggccg
    23461 cggcgaccag caggacgcga gcgggaaagc gcagcgcgac ctggccgcgc acgatgtcga
    23521 cgctgccacg ctcgagcggc tggcgcagcg cctcgagcgc cgggcgggcg aactcggcga
    23581 gctcgtcgag gaacaggacc ccgcggtgag ccagcgtcac ctcgcccggg cgcggcctgg
    23641 tgccgccgcc gacgagcccc tgggcggaga tcgtgtggtg cggcgcccgg aacggtcgct
    23701 cgctcgccaa cccgtcggcg ccgccggtga cgctctggac gcgcgtgatg tcgagcgcct
    23761 cctcgatcgt cggcggcggc aggatcccgg ccaggcggcg ggcgagcatc gtcttgccgg
    23821 cgcccggcgg tccgaccatc agcaggctgt ggccgcccgc cgcggcgatc tccagcgcgc
    23881 gacgggcgtc cgactggccg cgtacgtcgg cgagatccgg aagctggtcc gccggctgcg
    23941 ccggcgctcg acgccgggca gcgggcggct ccggcgccca gcggtcgtga agcagctcga
    24001 cgagccgacg caggtcgggt accgcgcaga cctcgaggtc gtcggccaag ctcgcctcgg
    24061 gcgctatctc aggcgcgagg acgagtcgac ggatgccggc cgcgcgacag ccgagcgcga
    24121 tcggcagggc gccgcgcacc ggccgcagcg agccgccgag cgacagctcg cccgagactg
    24181 cgcagccgtc cagggcgctg gccggtacct gtccgctggc agcgaggact ccgacggcga
    24241 tcgccaggtc gaagctcgct cccgccttgc gcacccaggc tggggcgagg ttgacggtca
    24301 ggcgctggag cggaaactcg agttcgctgt tgagcagcgc cgagcgcacg cgctcgcgcg
    24361 cctcgcggac ggcggcgtcg ggcagcccga cgaccgtgaa tgcgggcagg ccgcgacggg
    24421 cgtcaacctc gacggtgacc tctcgggcat cgacgccctc gatggcgaac gttggcacgc
    24481 tggcgagcac ggagggcacg ctaggcaggc ccgtcgcaca ggtgacgccg cttccgcgac
    24541 gagaccgaca cagccggctc tgcgtcctgg agccgacctg tcacaccagg gcgccggcgg
    24601 gcgcaaacgc cgatctagcg tctcgcggat ggccggcggc gacacccgca tccagctcgg
    24661 cgcgcacggc gaggagctcg cctgcgccca cctacggcgc gccggctaca cgctgatcga
    24721 gcgcaacggc cgctgcggcc ggatcggcga gctcgacgtc gtggcacgcc acggccgctg
    24781 cctcgtcttc tgcgaggtca agacccggct ggcgggcggc gtcgccggac cggccggacc
    24841 gctcgacgcg atcggacccg cgaagcagcg gcggatccgc cggctcgccg ccgaatggct
    24901 gcgcacccgc ccccagagcg gcggggcgcc gtggatccgc ttcgacgcga tcggggtcct
    24961 cgtcgaccgc cagggccgcc tgctcgccct cgaccacgtc gaggacgcct tctagccgag
    25021 cgtcagctgc tggtaggcgg tcgagttgaa cgacatgcga tggatcggcg agacgccgtg
    25081 ggcggcgatc gcggcgcggt ggtcgggcgt cgagtagccg acgtgggccg cgaagtccca
    25141 ctcggggtgg cgctcggcga cacgtcgcat gtagcggtcg cgactgacct tggcgagcac
    25201 ggaggcggcg gcaatcgcag cgctgcgcgc gtcgccgtcc accacggcac gctgctcgtg
    25261 ctcgaagggc ggcacccgga atccgtcgac gaggcagacc gtccccggca ccgcgacccg
    25321 tcgcagcgcc gatctcagcg cgtcgaggtt ggtcacgtgc aagccgcgcg agtcgatgcc
    25381 gcgcacgcag cgcgacacca cggcggtcct ggcgcacgct cgcatcacca gcgggtacag
    25441 ctcctcgcgc gcctcctcgc tgtgctgctt ggagtcgttc aggcgcgcca gcgcgcgccg
    25501 atcgccgagc gtcagccgct cgaggtcgaa cagcaccgcc gcggcgacca acgggcccgc
    25561 gaggcaccct ctcccggcct cgtccgctcc cgccacgaac cgcgccccca ggcgccggtc
    25621 atggttgaag
//
LOCUS       DATABV010000184        19039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2788377, whole genome shotgun sequence.
ACCESSION   DATABV010000184 DATABV010000000
VERSION     DATABV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 19039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19039
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788377"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..376)
                     /locus_tag="VHF88_08305"
     CDS             complement(20..376)
                     /locus_tag="VHF88_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161807.1"
                     /translation="MSLLWKFGLIALIALVLTAAPGGGDALNLLLMALSLAFFTAIAL
                     LGYRLFHQFRSDLEGLPDRDRAVLYGSIGLAFLTFCATSRLFDVGGGGVVVWLALLAA
                     CSYGVYWVWTRYRAYE"
     gene            complement(387..1220)
                     /locus_tag="VHF88_08310"
     CDS             complement(387..1220)
                     /locus_tag="VHF88_08310"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX2161808.1"
                     /translation="MSLIEAIVLGILQGLTEFLPISSSAHQLIVPAMLGWDDPGAAFT
                     AVTQLGTEAAVLIYFRRDIWSIASVWTRSLFQPVLRSTAHARMGWYLIVATIPIGILG
                     LAFEDQIENGARNLWLVGTVLIVFGLVLGYADRVGTHERELGRISTRDGILIGLAQSL
                     ALIPGVSRSGATMSAGLLLGLERAAAARFSFLLAVPAVVLSGLFQLAGILSGEEGDGD
                     PLGYILLATVISFFVGYVAIAWLLRYLTTHSVGVFVGYRIALGTLVLALTAAGAIQSP
                     G"
     gene            1281..1535
                     /locus_tag="VHF88_08315"
     CDS             1281..1535
                     /locus_tag="VHF88_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161809.1"
                     /translation="MKARIALLASLALTGWLLYAVPALAHDNGEGLLGETNDKIITFF
                     SLGVVVFFAVLVSVLSWLQAVLDRRKEQRKATAVRDIVGW"
     gene            1550..2326
                     /locus_tag="VHF88_08320"
     CDS             1550..2326
                     /locus_tag="VHF88_08320"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX2161810.1"
                     /translation="MPLDAEQVTYERRGAAAVVTIDRQERRNAVDGPTADLLAEAHDR
                     FEQDDGARVMVLTGAGDEAFCAGADLKAIETLGPRLDSPDGPLGFTRRTPSKPAIAAI
                     SGWCLAGGLELALWCDLRIAAENAKLGFTERRFGVPLIDGGTQRLPRIVGLGRGLDMI
                     LTGRIVGPQEALGMGLLTEVVAPGRQLERALEMADALAVFPQQTMLADRRAAIEGLGL
                     PLADGLRLEAESGPATFSDARAGADRFTSGEGRGGTGTGA"
     gene            complement(2392..2748)
                     /locus_tag="VHF88_08325"
     CDS             complement(2392..2748)
                     /locus_tag="VHF88_08325"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2161811.1"
                     /translation="MSVDLELAPKQKNAPGVRCCEPVVYPDVDRAEAERMASIAKALA
                     DPVRVQLVDVLRKHAGRVCVCELVPLFDLSQPTVSHHLGVLRKAGIVASERQGLWAYY
                     YVNSEALEEVSAWLTG"
     gene            2844..3707
                     /locus_tag="VHF88_08330"
     CDS             2844..3707
                     /locus_tag="VHF88_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2161812.1"
                     /translation="MSAPAIEVSDLRKSYGDVDAVRGLSFEVARGEVFGILGPNGAGK
                     TTTVEILEGYRRRSGGSVSVLGHDPERRERQLRQRVGIVLQSCGFYPRLTVREAVGHM
                     AKAYPAPLDTDETIGLVGLGDKADTHAGKLSGGQQRRLDLALALVGDPELVFLDEPTT
                     GFDPAARRDAWEVVRRLRELDKTVLLTTHYLDEAQELADRVAIVKDGAVVAEGPPSDL
                     KPDSGGYRVSYLSGGRRVEHQTEDPTDLLHRLTGDALGRGERLEGLEVARPTLEDVYL
                     ELTAEAEEERS"
     gene            3704..4441
                     /locus_tag="VHF88_08335"
     CDS             3704..4441
                     /locus_tag="VHF88_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2161813.1"
                     /translation="MSGVALAWAQFRIERKLFWRNPSAAFFSFILPLTLLLLVASVFA
                     DDPSELDVLIPGVAGMSVMATTFNAMAYNLTFLREQGILKRMRGTPMPTASYFAGFLG
                     NAVANAFVQVGLVIVIGHFLYDVAWPKDWFELAVFTAVGVVTFGSLGIALSHLIPNFD
                     SASAYVNALFLPAIMISGVFYSSDSLPAGLAAVAEALPLKHVIDGFSDAIVTGRGLSH
                     ELGALAVLGLWGAAGLLLAIRGFRWER"
     gene            complement(4526..5686)
                     /locus_tag="VHF88_08340"
     CDS             complement(4526..5686)
                     /locus_tag="VHF88_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161814.1"
                     /translation="MGDQLDSVLSREGWAHASGAPAVGGDPGRFNTLFGRDSLITALQ
                     VLPERPDVAEATLRALAALQGRRDDPRTDEEPGKILHEHWRQAPRRLVDRAWPVRGDG
                     GLTYYGSADSTSWFLVLLDVLDDARLAMELEPNWRAAGEWLLRAVERGGGLVRHGPRR
                     APGGLAQQGWRDTTDPLDPDTRGGGILREDGSAPQPPLADADTQAVAVAAAAALARLS
                     GEQRHRDLAESLRAEVVRSFTPDTLAIEGDGRPVSGAGSQLGWLLWAGAAPPGAAERL
                     VQPDVLTPWGLRTLSADHPQFDAHAYHRGAVWPFDCWLGWGGLRAAGREQDAERVRTG
                     VLEALDRLGGAPELYAVTPDGPQPIAIANPLQAWTVGARWAFENDWNGRGQP"
     gene            complement(5698..6609)
                     /locus_tag="VHF88_08345"
     CDS             complement(5698..6609)
                     /locus_tag="VHF88_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2161815.1"
                     /translation="MEASPQTVARPGTAVAGSRARVAEARGPRRSRLRPALLWIAVVL
                     ICVFCLMPFYWLINVSLKTGTDLSSADLLPPNPSLDNYQSVFQNADFTDALRNSVIIT
                     TITTTLALVFGSFAAYALARLRFRFKFLILAIVLSISTFPPIAIAAPIFKLWTDIGLY
                     NTYTGLIVPYLTFALPLAIYILASFFKEIPKELEEAALVDGATNFQAFRKVVLPLAAP
                     GLVTAGLLTAFFVWNEFLLAVTLTSSPSAYTVPVAVANFTGSTEFEVPLGTISAASVI
                     ITIPLVALVIIFQKRIVAGMTAGAVKG"
     gene            complement(6609..7550)
                     /locus_tag="VHF88_08350"
     CDS             complement(6609..7550)
                     /locus_tag="VHF88_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2161816.1"
                     /translation="MEASTARLGRRRSRGRDRYGSGERRLALAMLSPSMVVIALVAAY
                     PIGYAIWLSLNEYSVRVPGLSRFAGFDNYTEAFASPEFWQAALVTFLFTVISVGLELV
                     VGLGMALAMHEAFRGRGILRTVVLVPWAVLTVVSAITWRTIFEPNLGLAPQIIDALAL
                     PGAGVVWLGEEGYSLAVMVLADVWKTAPFMALLLLAGLQVIPNDVYDAAKVDGATTLQ
                     RFRRITLPLLAPAILVALIFRTLDALRIFDLPYVLTRGANNTTTLSLLAHQELTSNRL
                     IGYGSALSVLTFVIVMAVSFLYIRTVGGNIRSLVEDR"
     gene            complement(7577..8860)
                     /locus_tag="VHF88_08355"
     CDS             complement(7577..8860)
                     /locus_tag="VHF88_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2161817.1"
                     /translation="MNRLSVLATLVAVVLLAFAGCGDDDGGGGGGAGAGDAPDAEQVG
                     QARAEGDVTWCIGKDTSGAFTTVVDNFNKANPNANVELLELPESADDQRRLQIQRLRA
                     ESPQCDVLGMDVIWTAEYAAQNWLLDMTDAVEEQRDAFIASTLETAMYQDKYWAFPFN
                     TNAGFIYWRTDQAGNEPPQSWEDMFQVAQQENGYIFQGARYEGLTVNFLELLYSAGGS
                     VVNEDGSEATADSQQVRDVLAFMRKGVDDGAIPKAVTTYMEEESRRAFEAGRATFMRN
                     WPYAYALAKESRIADDFDVTTFPSYQGNEGAGVLGGYNLAISAFSENPEGALAFIEFA
                     TRPDQQRIQVTKASLPAVVSEVYDMAAVKQALPFATELRDAIEQAQPRPVSPVYPQIS
                     EAIYTNVYSALQGDKSPDQAAQDMNGDIQQALETF"
     gene            complement(8932..9621)
                     /locus_tag="VHF88_08360"
     CDS             complement(8932..9621)
                     /locus_tag="VHF88_08360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX2161818.1"
                     /translation="MSATPYTVRHSDRARRARIQVTAEGVEVVVPRRLPLREVEPFVD
                     SKRAWIERTLLRMRTAAAQRPRPRLEDGGELPFLGERLRLRVRVEPARRRAHVARRGE
                     ELTVAVAEDHPEAIGAALERWYRAQARAEVGRRLDAAVARVGRSYAGLQIRGQRTRWA
                     SCSSSGAMSFNWRLLLAPPEILDYVVEHEVAHLDVHDHSPRFWALLAQRMPDCRRHER
                     WLRANGQTLLL"
     gene            complement(9657..10445)
                     /locus_tag="VHF88_08365"
     CDS             complement(9657..10445)
                     /locus_tag="VHF88_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2161819.1"
                     /translation="MDLGLRDRACVITGGSRGIGLATARLLSGEGARVLLVARSESDL
                     AAAAEQCDGRAEILAADITDPDAGEHVVTTCDQRFGRVDALVNNAGTTKMVALDELTD
                     ADWQEQWELHVMASMRLMRAAAPRMAAAGWGRIVNVCSSSGKRPSQRNAAYSVTKAAQ
                     LSLSRTYADAFAPQGVLVNAVAPGVVGTGLWLDEGGLADQVAAQQGVDRDEVIGGLGD
                     KIPLGRIGTEEEIAAVIAFLCSERASNVAGAAWSVDGGTVPTIL"
     gene            10673..12883
                     /locus_tag="VHF88_08370"
     CDS             10673..12883
                     /locus_tag="VHF88_08370"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX2161820.1"
                     /translation="MADTVTNANDSGPGSLRAVIAGADGGDTIDFHPSLDGETIELTS
                     GAIEIDKRLTIAGPDASQLTIDANQGGRIFATVSDDDPSTDENLSISGLTLANGVAPD
                     DGVLLRSGGAIHHGSTGSLTISDSTFSGNRAGGAGGASDNSGRGRGGAIHVGLTSGPV
                     VISGSRFVGNSAGGPGGDGLESGRGWGGAIHIGTNSTVSVSDSIFADNSAGGTGGAGH
                     DSGHGAAGAISMSGGSPSTFTVTRSRFSGNATGGDGGDGIGSGGGQGGAITISLNSAT
                     ISDSTFDANSAGGDGGGSPSGGRGVGGAIYTSGTKPLAITNSTLAGNSAGGDGARGFG
                     GALSLSPAATATLDSVTIAANSVGDHLESEGAAIGDSKPLIVDFAEVTARATIISGNS
                     GASDCNMPVVSSRYSMEGPSATDTSCGLDLDSADPQLAPLADNGGPTETQALAPASPA
                     VDVVPKALCPTVVDQRGEPRPEPGELLCDVGAFERQDPVAPTITSIAAATFQVGKAGS
                     FTVAARGGPKPSLSQTGALPGGLSFTDNGDGTASLAGTPVDGTAGVYPIAIKASNGVT
                     PDVTQSFTLTVQAAPPVPPQPPPPGPPGPPNPPGPPSPPGPPGPPGPPEPELPLELST
                     DVKRKSLAKLVRSRRLVVAANVNGAARLTLTGRAKLGIRVGGKLKTRLVVVFRQKAVR
                     FSAAGERKVTLALTRKGRRALRRLEKVRLVVVAKATATTGETKRARVALTLRRS"
     gene            complement(12880..13803)
                     /locus_tag="VHF88_08375"
     CDS             complement(12880..13803)
                     /locus_tag="VHF88_08375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX2161821.1"
                     /translation="MARCFVTREMPGAALERLAAEHDVEVWPERLPPTPQQLHERAAE
                     AEGLLCMLTDPVDRELFDAAPRLRAVSTYAVGTDNIDLAAAAERSIPVGHTPDVLTET
                     SADLALALMLAVMRRIPAGDAEVRAGEWLTWEPARLLGHDLHRATVGIVGLGRIGRAV
                     ERRVEGFGARVVHSGRTDGVPLEQLLEQSTVVTLHCPLTDATRGLIDADALRRMRDDA
                     YLVNTARGPIVDTDALLRALEEGWIAGAALDVTDPEPLPAEHPLLRAPNLVVAPHLGS
                     ASHETRAEMAEIAVDNLLAGLRGDPLPHQAG"
     gene            13895..14404
                     /locus_tag="VHF88_08380"
     CDS             13895..14404
                     /locus_tag="VHF88_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161822.1"
                     /translation="MPKDTGFPRTDAQSDFTRARRRRALARLANRLRGKPGAKVAILP
                     FEEVVEALGRRGERRLGLRSIALDSIVGTVDRGREFDRGFRPTSPRPRGRFERIATAK
                     RRGEPMPPIRVYRIANAHFVEDGHHRVAVARAQGQPDIDAYVTEVVTEVGHDLDLRSV
                     TPDELRALR"
     gene            complement(14418..15611)
                     /gene="ugpC"
                     /locus_tag="VHF88_08385"
     CDS             complement(14418..15611)
                     /gene="ugpC"
                     /locus_tag="VHF88_08385"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX2161823.1"
                     /translation="MAKIVFDGITKRYPDGYEAVKDMSLEVEDGEFMILVGPSGCGKS
                     TALRMVAGLEDISEGQLFIGDDVVNQKAPRDRDIAMVFQNYALYPHMTVRDNMGFALK
                     LHGVDKAEIDKRVEEAAKILDLAEHLDRKPANLSGGQRQRVAMGRAIVRDPSAYLMDE
                     PLSNLDAKLRVQMRTEVSRIQNRTDTTTIYVTHDQTEAMTLGDRVAVMRAGKLQQVDT
                     PMELYNRPKNLFVAGFIGSPAMNFMPATIEGDTVKLPMVDVRLPEDLRRRIGQGDAAG
                     RELIAGIRPENFEDAALLGDDARGRGATFRAKIDMIEAMGSELYAHFRVTSDQRIESE
                     ELRELAEDSGVGEVPGAGEEGEIVARLSAESQVSRGEEAELFIDASHIQLFDPQDGSS
                     LTAGG"
     gene            complement(15670..17019)
                     /locus_tag="VHF88_08390"
     CDS             complement(15670..17019)
                     /locus_tag="VHF88_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEX2161824.1"
                     /translation="MIEQREADVAIVGAGLAGLVAARDLLAAGASVVVLEARDRVVEV
                     GGQWIGPTQDRIAALADEMNVETFPTYAIGDNVIEWRGALRRYRGTIPRVSPLVLLDV
                     LRTQRRLDRMARTVPLDRPWLASKAAVWDGQTASTWLRRNVLTKGGRDLLQLGTEAVW
                     AAQPEDVSLLHMLFYIHSAGSLERLLDTAGGAQQDRLVGGSQRVPIALAQRLGDDVVV
                     LDAAVRRIEHGGDGVTVHADRVGDAAGAGDDRLTVRARRAIVAIPPTLAGRIVYDPPL
                     PGHRDQLTQRMPLGTVAKCMAVYDEPFWRGDGLSGQGLSDTGPIKLTYDNSPPAGRPG
                     VLLGFLEGRQARELGRLGASDRRAAVIGCFTRLFGARAARPDRYVERLWAEEEYSRGC
                     YGCHMPTGAWTGYGEALRAPIGPLHWAGAETAEVWSGYMDGAVRSGESAANELLAAL"
     gene            complement(17077..17487)
                     /locus_tag="VHF88_08395"
     CDS             complement(17077..17487)
                     /locus_tag="VHF88_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2161825.1"
                     /translation="MGNVTQGEGYSVANLDDIGEGPGFRKIRRELGVEEFGINAIVMP
                     SGIESDFHYHDEQEELYFVHSGELEIEFGDGTVHRLGPGGMARVAAATHRKTRNVSGG
                     DTVYVIVGAKGGYVGRDGQAPDGNGLARVRRLPA"
     gene            17538..18284
                     /locus_tag="VHF88_08400"
     CDS             17538..18284
                     /locus_tag="VHF88_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX2161826.1"
                     /translation="MIRIGCSGWNYDSWRGGLYPEGMGPARWLREYARHFDTVEVNST
                     FYRLASRNGVERWVAETPDDFCFAVKGSRYLTHVKRLADAPDKDFVLGKGIARFYERI
                     TPLIPAGKLGPVLWQLPPSFKRDDERLVNLLELVPPGRHCVEFRHPSWFAEDVYRLLR
                     AHGAALVIADRPELTFQTHELTTDWTFVRFHYGHRGRRGNYSRSELEQWAPRIAGWHD
                     RGVDVYAYFNNDWEVFAPRNAATLKRLLDV"
     gene            complement(18294..>19039)
                     /locus_tag="VHF88_08405"
     CDS             complement(18294..>19039)
                     /locus_tag="VHF88_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2161827.1"
                     /translation="VGWRGLHRFFRDPTMVVPSMLFPLFYFGAFAGGLSAVSDVPGFD
                     FPDNYATFQFVFVLLQASAFGGVFTGFAIARDFEGGVGRRMMLAAPNRRALLAGYALV
                     AAGRALFIWIMLTVLALAVGMEVGGGVADLVGMYTLALLMNLTALMWAAGIALRFRSL
                     QAGPLMQTPVFLVVFIAPVYVPFDLLTGWVKTAASVNPITAVIEAARDFISGAPADVA
                     LAYGAAAGMLVFFGVWALRGLAKAEAAGG"
BASE COUNT         2888 a         6778 c         6724 g         2649 t
ORIGIN      
        1 cccccccccc cccttcttct cactcgtaag cccggtagcg cgtccagacc cagtagacgc
       61 cgtacgagca ggcggcgagc agcgccagcc agaccacgac gcccccgccg ccgacgtcga
      121 acagacgact cgtcgcgcag aaggtgagga acgccaaccc gatcgacccg tagaggaccg
      181 cccggtcgcg gtccggcaga ccctcgagat cgctcctgaa ctggtggaag aggcggtagc
      241 cgagcagggc gatcgccgtg aagaacgcca gcgacagagc catcagcagg aggttgagcg
      301 cgtcgccgcc gcccggcgcg gcggtcagca cgagcgcgat caacgcgatc agaccgaact
      361 tccagagcag gctcaccggc tcgatcctag cccggcgact ggatcgcgcc ggccgcagtc
      421 agcgcgagca cgagcgtgcc gagggcgatc cggtagccga cgaacacacc cacagagtgc
      481 gtggtcagat agcgcagcag ccaggcgatc gccacgtagc cgacgaagaa cgagatcacc
      541 gtcgcgagca ggatgtagcc gagcgggtcg ccgtcgccct cctctcccga caggatgccg
      601 gcgagctgga acaggccgct gagcacgacc gccggcacgg ccagcaggaa cgagaaccgg
      661 gccgccgccg cgcgctcgag cccgagcagc aggccggcgc tcatcgtcgc gcccgaccgc
      721 gacacaccag ggatcaacgc gagcgactgc gccagtccga tcaggatgcc gtcgcgggtc
      781 gagatccgcc ccagctcgcg ctcgtgggtc ccgacgcgat cggcgtaacc caggaccagc
      841 ccgaagacga tcagcaccgt cccgaccagc cacaggttgc gggcgccgtt ctcaatctgg
      901 tcctcgaacg ccagcccgag gatgccgatc gggatcgtcg cgacgatcag gtaccagccc
      961 atgcgggcgt gggccgtaga gcgcaacacc ggttggaaga gcgagcgcgt ccagaccgac
     1021 gcgatcgacc agatgtccct gcggaagtag atcagcacgg cggcctcggt tccgagctgc
     1081 gtcacggcgg tgaaggccgc gcccggatcg tcccagccga gcatcgccgg cacgatcagc
     1141 tgatgggcgc tcgacgagat cggcaggaac tccgtgagtc cctgcaagat gcccagcacg
     1201 atggcctcga tcagcgacac ggccgccgaa ccctagtcag aagggggcgc tccgccccag
     1261 ccggtaaact gccgcgtcgg atgaaggccc gcatcgccct gctcgccagc ctcgccctca
     1321 cgggctggct gctctacgcc gtccccgcac tcgcgcacga caacggcgag ggtctcctcg
     1381 gcgagaccaa cgacaagatc atcaccttct tctcgctcgg cgtggtcgtc ttcttcgccg
     1441 tgctcgtctc cgtgctctcc tggctccagg cggtcctgga ccgccgcaag gagcagcgca
     1501 aggcgaccgc ggtacgcgac atcgtcggct ggtagcgctc ggcgcgggca tgccgctcga
     1561 cgccgaacag gtgacctacg agcgccgcgg cgccgcggcg gtggtgacga tcgaccgcca
     1621 ggagcggcgc aacgccgtcg acgggccgac cgcagacctg ctcgccgagg cgcacgaccg
     1681 cttcgagcag gacgacggcg cgcgggtgat ggtcctgacc ggcgcgggcg acgaggcgtt
     1741 ctgcgcggga gccgatctga aggcgatcga gacgctcgga ccgcggttgg attcgcctga
     1801 cggtccgttg gggttcaccc gccgcacgcc gtcgaagccg gcgatcgccg ccatctccgg
     1861 ctggtgcctg gccggcgggc tcgagctggc cttgtggtgc gacctgcgga tcgcggccga
     1921 gaacgcaaag ttgggcttca ccgagcgccg cttcggagtg ccgctgatcg acggcgggac
     1981 tcagcggctg ccgcggatcg tcggtctcgg ccgtggcctc gacatgatcc tcacggggcg
     2041 catcgtgggt ccgcaggagg cgctggggat gggcctgctg accgaggtcg tagcgcccgg
     2101 ccggcaactc gaacgagcgc tcgagatggc cgatgcgctc gccgtcttcc cccagcagac
     2161 gatgctcgct gaccgccgcg cggcgatcga gggcctcggc ctgccgctcg ccgacggcct
     2221 ccggctcgag gccgagtccg gccccgccac cttctccgac gcgcgcgccg gcgccgaccg
     2281 cttcacctcc ggcgagggtc gcggcggcac cggcacgggg gcctgaggtc tctacacggc
     2341 gtcagcagca gccgccctca tcgcagcatc cctccccgtc ggtgcgggta atcagccggt
     2401 caaccatgcg ctcacctcct ccaatgcctc gctgttgacg tagtagtagg cccacagccc
     2461 ctggcgctcg gaggcgacga tgccggcctt gcggaggacg ccgagatggt gagagacagt
     2521 gggctgggag aggtcgaaga gcgggacgag ctcgcagacg cagaccctgc ccgcgtgctt
     2581 gcgcaggacg tcgacgagct gaacccgcac ggggtcggcg agcgccttgg cgatcgacgc
     2641 catccgctcg gcctccgcac gatcgacgtc gggatagacg accggctcgc agcagcgcac
     2701 gccgggcgcg ttcttctgct tcggggcgag ctccagatcg acactcatct atctatgtat
     2761 atcaattcaa gtgatacatg tcaatgcaac caacggccct cgcgctgccg ggcggcccgc
     2821 ggcgccgact agagtgcgcg gccgtgagcg caccggcgat cgaggtctcc gacctccgca
     2881 agagctacgg cgacgtcgac gccgtccgcg gtctctcatt cgaggtcgcg cgcggcgagg
     2941 tgttcggcat cctcggcccc aacggcgccg ggaagacgac gaccgtcgag atcctcgagg
     3001 gctaccgcag gcgctccggc ggcagcgtca gcgtgctcgg tcacgacccc gagcggcgcg
     3061 agcgccagct gcgccagcgg gtcgggatcg tcctgcagag ctgcggcttc tacccgcgac
     3121 tgacggtgcg cgaggcggtc gggcacatgg cgaaggccta ccccgccccg ctcgacaccg
     3181 acgagacgat cgggctcgtg ggactcggcg acaaggccga cacccacgcc ggcaagctgt
     3241 ccggcggcca gcagcggcgg ctcgatctcg ccctcgcgct cgtcggcgac ccggagctgg
     3301 tcttcctcga cgagccgacc accggcttcg accccgcggc gcggcgagac gcctgggagg
     3361 tcgtgcgacg gctgcgcgag ctcgacaaga cggtcctgct gacgacccac tacctcgacg
     3421 aggcccagga gctcgccgac cgcgtggcga tcgtcaagga cggcgccgtc gtggccgagg
     3481 gcccgccctc cgacctcaag cccgactccg gcggctaccg cgtctcctac ctctccggcg
     3541 ggcggcgcgt cgagcaccag accgaggacc cgaccgacct cctgcatcga ttgaccggcg
     3601 acgcgctcgg gcgcggcgag cggctcgagg ggctcgaggt cgcgcgaccg acgctcgagg
     3661 acgtctacct cgagctgacc gccgaggcgg aggaggagcg gtcgtgagcg gcgtcgcgct
     3721 ggcctgggcg cagttccgca tcgagcgcaa gctgttctgg cgcaacccga gcgcggcttt
     3781 cttcagcttc atcctgccgt tgacgctgct gctgctggtc gcgtcggtct tcgccgacga
     3841 cccgagcgag ctcgacgtgc tgatccccgg cgtcgccggc atgagcgtga tggccacgac
     3901 cttcaacgcg atggcctaca acctgacctt cctgcgcgag cagggaatcc tcaagcggat
     3961 gcgcggcacg ccgatgccga ccgcctccta cttcgcgggc ttcctcggca acgccgtcgc
     4021 caacgcgttc gtccaggtcg gcctggtgat cgtgatcggg cacttcctct acgacgtcgc
     4081 ctggccaaag gactggttcg agctcgctgt cttcaccgcc gtcggcgtgg tgaccttcgg
     4141 gtcgctgggg atagcgctct cccacctgat cccgaacttc gactcggcgt cggcctacgt
     4201 caacgcgctg ttcctgccgg cgatcatgat ctccggcgtc ttctactcga gcgactcgct
     4261 gcccgccggg ctcgccgccg tcgccgaggc gctgccgctg aagcacgtca tcgacggctt
     4321 ctcggacgcg atcgtcaccg gccgagggct ctcgcacgag ctcggcgcgc tggctgtgct
     4381 cgggctctgg ggcgccgccg gcctgctgct ggcgatccgc ggctttcgct gggagaggtg
     4441 aacggcagct gcggtactcg ccggacctcg ctccagtgaa ggccgcgatg cgccgctgta
     4501 ggaccggccc ggctgccccc tccgcttagg gctggccccg cccattccag tcgttctcga
     4561 aggcccagcg ggcgccgacg gtccaggcct gcagggggtt ggcgatcgcg atcggttggg
     4621 gcccgtcggg cgtcaccgcg tagagctcgg gcgcgccccc gaggcggtcg agggcctcga
     4681 ggacgccggt cctgacgcgc tcggcgtcct gctcgcgacc cgcggcgcgc aggccacccc
     4741 agccgagcca gcagtcgaat ggccagaccg cgccgcggtg ataggcgtgg gcgtcgaact
     4801 gcggatggtc cgcggacagc gtgcgcagtc cccacggggt caggacatcg ggctggacca
     4861 gccgctcggc ggcgccgggc ggcgccgcgc cggcccacag aagccagccg agctgcgagc
     4921 cggcgccgga gaccggccgg ccgtcgccct cgatggcgag cgtgtccggc gtgaaggatc
     4981 ggaccacctc cgcgcgcagc gactcggcca ggtcgcgatg ccgctgctcg cccgagaggc
     5041 gggccaatgc agcggccgcg gctacggcca ccgcctgcgt gtcggcgtcg gccagcggcg
     5101 gctgcggcgc cgagccgtcc tcgcgcagga tcccaccgcc gcgggtgtcg ggatcgagcg
     5161 ggtcggtcgt atcgcgccag ccctgttggg cgaggccgcc gggcgctcgg cgcgggccgt
     5221 gacggacgag cccgccaccg cgctcgaccg ctcgcagcag ccactccccc gcggcgcgcc
     5281 agttgggctc gagctccatg gccaggcggg cgtcgtcgag cacgtcgagg agaaccagga
     5341 accacgaggt cgagtccgcc gagccgtagt aggtgaggcc accatcgccg cgcacgggcc
     5401 aagccctgtc gacgagcctg cgcggcgcct ggcgccagtg ctcgtggagg atcttcccgg
     5461 gctcctcgtc ggtccgggga tcgtcgcggc gaccctgcag cgccgccagc gcgcgcagcg
     5521 tcgcctccgc gacgtcgggg cgctccggca acacctgcag tgcggtgatc agcgagtcgc
     5581 ggccgaagag cgtgttgaag cggccggggt cgccgcccac ggccggcgcc ccgctggcgt
     5641 gcgcccagcc ctcgcgcgag agcacgctgt cgagctgatc gcccacccgc gcggggttca
     5701 gcccttgacc gcgccggcgg tcatgccggc gacgatccgc ttctggaaga tgatcaccag
     5761 ggcgacgagc gggatcgtga tgatcaccga cgcggcgctg atcgtcccga gcggcacctc
     5821 gaactcggtg ctgccggtga agttggcgac agcgaccggc accgtgtacg ccgacgggga
     5881 gctggtcagc gtcaccgcga gcaggaactc gttccagacg aagaacgccg tcagcagccc
     5941 ggcggtcacc aggccgggcg ccgcgagcgg caggacgacc ttgcggaagg cctggaagtt
     6001 cgtagcgccg tcgaccagcg ccgcctcctc gagctccttc gggatctcct tgaagaacga
     6061 ggcgaggatg tagatcgcca gcggcagcgc gaacgtcagg tacggcacga tcagcccggt
     6121 gtaggtgttg tagaggccga tgtccgtcca gagcttgaag atcggcgcgg cgatcgcgat
     6181 cggcgggaag gtcgagatcg acagcacgat cgccaggatc aggaacttga agcggaaccg
     6241 cagccgcgcc agcgcatagg ccgcgaacga gccgaagacg agcgcgagcg tggtcgtgat
     6301 cgtcgtgatg atcacgctgt tgcgcagcgc gtcggtgaag tcggcgttct ggaagaccga
     6361 ctggtagttg tcgagcgacg ggttcggcgg caggagatcg gcgctcgaga ggtcggtacc
     6421 cgtcttcagc gagacgttga tcagccagta gaacggcatc aggcagaaga cgcagatcag
     6481 gaccacggcg atccagagca gcgccggccg cagccggctg cggcgcggtc cccgggcctc
     6541 cgccacccgg gcgcgcgagc ccgcgacggc ggtgcccgga cgcgccacgg tctgcgggga
     6601 cgcctccatc agcgatcctc caccagcgag cggatgttgc caccgaccgt tcggatgtag
     6661 aggaacgaca ccgccatcac gatcacgaag gtcagcaccg agagcgccga gccgtagccg
     6721 atcagccggt tgctggtcag ctcctggtgg gcgagcagcg acagggtcgt ggtgttgttc
     6781 gccccgcgcg tcaggacgta gggcagatcg aagatgcgca gcgcgtcgag cgtgcggaag
     6841 atcagcgcga cgaggatcgc cggcgccagc aacggcagcg tgatgcggcg gaagcgctgg
     6901 agcgtggtcg ccccgtcgac cttcgcggcg tcgtagacgt cgttggggat cacctggagg
     6961 ccggcgagca gcagcagggc catgaacggc gcggtcttcc agacgtcggc gagcaccatg
     7021 accgcgagcg agtagccctc ctcgcccagc cacaccacgc ccgcgccggg cagcgcgagc
     7081 gcgtcgatga tctgcggcgc caggccgagg ttgggctcga agatcgtccg ccaggtgatc
     7141 gcgctgacga ccgtcagcac cgcccacggc accagcacga cggtcctcag gatcccgcgg
     7201 cccctgaacg cctcgtgcat cgcgagcgcc atcccgaggc cgacgaccag ctcgagcccg
     7261 accgagatga cggtgaagag gaacgtgacg agcgccgcct gccagaactc cggcgaggcg
     7321 aacgcctcgg tgtagttgtc gaagccggcg aagcgcgaga gccccggcac gcgcacgctg
     7381 tactcgttga gcgacagcca gatcgcatag ccgatcggat acgccgcgac cagcgcgatg
     7441 acgaccatcg acggcgacag catcgcgagc gccagccggc gctcgccgct gccgtaacgg
     7501 tcacgaccgc gagaccggcg ccttcccagt ctcgcggtcg atgcctccat ctagtcctcc
     7561 acggtccgag cgtcggctag aaggtctcca gcgcctgttg gatgtccccg ttcatgtcct
     7621 gtgccgcctg gtcgggcgac ttgtcgccct gcagcgcgga atagacgttg gtgtagatcg
     7681 cctcgctgat ctgcgggtag accggcgaga ccgggcgcgg ctgcgcctgc tcgattgcgt
     7741 cgcgcagctc ggtggcgaat ggcagcgcct gcttgaccgc ggccatgtcg tagacctcgc
     7801 tcaccaccgc ggggagcgac gccttggtga cctggatccg ctgctggtcc ggcctggtgg
     7861 cgaactcgat gaaggcgagc gcgccctccg ggttctccga gaacgcggag atcgcgaggt
     7921 tgtagccgcc gagcacgcct gctccctcgt tgccctggta tgaggggaac gtcgtcacgt
     7981 cgaagtcgtc ggcgatccgt gactccttgg cgagcgcgta ggcgtagggc cagttgcgca
     8041 tgaaggtcgc gcgaccggcc tcgaaggcgc ggcgcgactc ctcctccatg taggtcgtca
     8101 cggccttcgg gatggcgccg tcgtcgactc ccttgcgcat gaaggccagc acgtctcgga
     8161 cctgttggct gtcggcggtc gcctcgctgc cgtcctcgtt gacgaccgag ccgccggcgc
     8221 tgtagagcag ctcgaggaag ttcaccgtca gcccctcgta gcgcgcgcct tggaagatgt
     8281 agccgttctc ctgctgggcg acctggaaca tgtcctccca gctctgcggc ggctcgttgc
     8341 cggcctggtc ggtgcgccag tagatgaagc cggcgttcgt gttgaacggg aaggcccagt
     8401 acttgtcctg gtacatcgcg gtctcgagcg tcgacgcgat gaacgcgtcg cgctgctcct
     8461 cgacggcgtc ggtcatgtcg agcagccagt tctgcgcggc gtactccgcc gtccagatca
     8521 cgtccatgcc gagcacgtcg cactgcggcg actcggcgcg caggcgctgg atctgcagcc
     8581 gccgctggtc gtcggcggac tcgggaagct cgagcagctc gacgtttgca ttcggattgg
     8641 ccttgttgaa gttgtcgacg accgtcgtga aggcgccgct ggtgtccttg ccgatgcacc
     8701 acgtcacgtc gccctcggcg cgcgcctgcc ccacctgctc ggcgtctgga gcgtcaccgg
     8761 cgccggcacc gccaccgcca cccccgtcgt cgtcgccaca gcccgcgaag gcaagtagga
     8821 ccacggcgac cagggtcgcc agaacgctca gccggttcat cgcacagctc ctctcgtagg
     8881 cgcttcctgc tccggttctt acccccggga cggggggtca aacgaagcgc ttcacaagag
     8941 cagcgtctgc ccgttcgcgc gcagccagcg ctcgtgccgg cgacagtcgg gcatgcgctg
     9001 ggcgagcagt gcccaaaaac gcggcgagtg gtcgtgaacg tcgaggtgcg cgacctcgtg
     9061 ctcgacgacg tagtcgagga tctcgggtgg cgcgagcagc agtcgccagt tgaagctcat
     9121 cgcgccgctg gacgagcagc tcgcccagcg tgtgcgctgg ccgcggatct gaaggcccgc
     9181 gtaggagcgt ccgacgcggg cgaccgcggc gtcgagccgg cggccgacct cggcgcgcgc
     9241 ctgggcgcgg taccagcgct cgagcgcggc gccgatcgcc tcgggatggt cctcggcgac
     9301 cgcgaccgtg agctcctcgc cgcggcgggc gacgtgggcc ctcctacggg cgggctccac
     9361 gcgcacgcgc aggcgcagcc gctcgccgag gaacggcagt tcgccgccgt cttccagccg
     9421 cgggcgcggc cgctgcgccg cggcggtccg catccgcagc agcgtgcgct cgatccaggc
     9481 ccgcttcgag tcgacgaacg gctctacctc gcgcagcggc agcctgcgcg gcacgacgac
     9541 ctcgaccccc tcggcggtca cctggatccg cgcgcggcgg gcgcggtccg agtggcgaac
     9601 cgtgtagggg gtcgctgaca cggccacgag gctaggcgac ggtccggcgg gcagcctcag
     9661 aggatcgtcg gcacggtgcc gccatcgaca ctccatgcgg cgccggcgac gttcgacgcc
     9721 cgctcggagc agaggaacgc gatcacggcg gcgatctcct cctcggtgcc gatccgcccg
     9781 agcgggatct tgtcgccgag gccgccgatc acctcgtcgc gatcgacgcc ctgctgtgcg
     9841 gccacctgat cggcgagccc gccctcgtct agccagaggc ccgtgccgac gacgcccggg
     9901 gcgacggcgt tgacgagcac gccttggggc gcgaacgcgt ctgcgtaggt gcgggaaagc
     9961 gacagttgcg cagccttcgt gaccgagtag gcggcgttgc gctgtgaagg ccgcttgccg
    10021 gaggaggagc agacgttgac gatccgtccc cagcccgcgg cggccatgcg gggtgccgcc
    10081 gcgcgcatca accgcatcga cgccatcacg tgaagctccc actgctcctg ccagtcggcg
    10141 tcggtgagct cgtcgagcgc caccatcttc gttgtcccgg cgttgttaac gagggcgtcg
    10201 acgcgaccga agcgctgatc gcaggtcgtc acgacgtgct cgccggcgtc agggtccgtg
    10261 atgtcggcgg cgagaatctc ggcgcgaccg tcgcactgct cggcggcggc agcgagatcg
    10321 ctctcggagc gcgcgaccag aagcactcgc gccccctcgc cggacagcag ccgtgccgtc
    10381 gccaagccga tcccccggct gccgccggtg atcacgcagg ccctgtcgcg cagtccgaga
    10441 tccatcggcg ggagtttcgc agtcgaccgg gcggggtcgc gccacgcgcg ctgggtgtcg
    10501 ccgagatcgc ctacgatggc tttcgcaaac ccgatcgtct gcgaatgggc ttgcaggacc
    10561 ggagatgctc tcgaccgtca cggagacaat cagtcacgga cgccgtctca gaggactcgc
    10621 gatagcggtc gcggcggtct gcctgatcgg ctccgcctcc agcgcacagg cgatggccga
    10681 caccgtgacg aacgcgaacg actcgggccc cggttcgctg cgagcggtga tagccggcgc
    10741 cgacggcggg gacacgatcg acttccaccc gagcctcgat ggcgagacga tcgagctgac
    10801 ctcaggggcg atcgagatcg acaagaggct gacgatcgcc ggccccgacg catcacagct
    10861 cacgatcgac gcgaaccagg gcggccggat cttcgccacc gtcagcgacg acgacccctc
    10921 gaccgacgag aacctgtcga tctccgggct taccctcgcc aacggcgtgg cgccagacga
    10981 cggcgtgctg ctccgcagcg gcggcgcgat ccaccacggg agcaccgggt cgctgacgat
    11041 ctccgactcg accttcagcg gcaaccgcgc gggcggcgcc ggcggagcca gcgacaacag
    11101 cggccgcgga cgcggtggag cgatccacgt gggcctgaca tccgggccgg tcgtcatatc
    11161 gggcagtagg ttcgtcggga actcggccgg cgggccgggc ggtgacggcc tcgagagcgg
    11221 ccgcggctgg ggcggcgcga tccacatcgg gaccaacagc acggtgagcg tctcggacag
    11281 catcttcgcc gacaacagtg ccggtgggac tggcggcgcg ggccacgaca gcggccacgg
    11341 ggcggctggc gcgatcagca tgagcggggg cagccccagc acgttcacgg tcactcgcag
    11401 caggttctcc gggaacgcca ccgggggcga cggcggcgac gggattggca gtggcggcgg
    11461 acagggaggc gcgatcacga tctcgctgaa ctcggccacg atctccgaca gcacgttcga
    11521 cgccaacagc gccggcgggg acggcggcgg cagcccttcc ggcggccgcg gcgtcggcgg
    11581 cgcgatctac acctcgggga cgaagcccct cgcgatcacc aacagcacct tggccgggaa
    11641 cagcgccggc ggcgacggcg cgcggggctt cggtggcgcg ctttcgctct cccccgccgc
    11701 gacggcgacg ttggacagcg tgacgatcgc tgccaactcg gtcggcgacc acctggagag
    11761 cgagggcgcc gcgatcggcg actccaagcc gctgatcgtc gacttcgcgg aggtgaccgc
    11821 gagggcgacg atcatctccg ggaacagcgg cgcgtcggac tgcaacatgc ccgtggtctc
    11881 gtcgaggtac agcatggagg ggccgtccgc gaccgatacg agctgcggcc tggacctcga
    11941 ctcggcagac ccgcagctcg cgccgctcgc cgacaacggc ggtccgaccg agacgcaggc
    12001 gctcgcaccg gcgagtcccg cggtcgacgt cgtccccaag gccctttgcc cgaccgtcgt
    12061 cgaccaacgc ggcgagccgc ggcccgagcc tggggagctc ctctgtgacg tcggcgcgtt
    12121 cgagcgacag gatcccgttg cgcccaccat caccagcatc gccgcagcga ccttccaggt
    12181 gggcaaggcg gggagcttca ccgtcgcggc gaggggtgga ccgaagccgt cgttgtcgca
    12241 gacgggcgcg ctgcctggtg ggttgagctt caccgacaac ggcgacggca ccgcgagcct
    12301 cgccggaacg ccggtggacg gcacggccgg ggtctacccg atcgcgatta aggcctcgaa
    12361 cggcgtgaca cccgacgtga cgcagagctt cactctgacc gtacaagcgg cgccgccggt
    12421 gccgccgcag cccccgccgc ccggtccgcc agggcctcct aatccgccag ggcctcccag
    12481 tccgccagga cctccgggcc cgccagggcc gccggagccg gagctgccgt tggagctctc
    12541 cacggatgtc aagaggaagt cccttgcgaa gctcgtccgg agtcggaggc tcgtcgtcgc
    12601 tgcgaatgtc aatggagcgg cgaggttgac cctgacgggt agggcgaagc tcgggatccg
    12661 cgtgggcggc aaactgaaga cgaggctcgt cgtggtattc aggcagaagg ccgtccggtt
    12721 cagcgccgct ggtgagagga aggtcacgct ggccctcacc cgaaagggac gcagggcgct
    12781 tcggcgtttg gagaaggtga ggctggtggt cgtcgccaag gcgaccgcca ccaccggtga
    12841 gacgaaacga gcgcgggtcg ccctgaccct ccggcggtcc tagccggcct ggtgcggcag
    12901 cggatcgccg cgtaggccgg cgagcaggtt gtcgacggcg atctcggcca tctcggcgcg
    12961 ggtctcgtgc gacgccgagc caaggtgcgg cgcgacgacg aggttcggcg cgcgtagcag
    13021 cggatgttcg gccggcagcg gctccgggtc tgtgacgtcg agcgcggcgc cggcgatcca
    13081 gccctcctcc agcgcccgga gcagcgcgtc ggtgtcgacg atcggcccgc gggcggtgtt
    13141 gacgagatag gcgtcgtcgc gcatccggcg cagcgcgtcg gcgtcgatca ggccacgcgt
    13201 cgcgtcggtc agggggcagt ggagcgtcac gactgtcgac tgctccagca gctgctcgag
    13261 cggcacgccg tcggtgcggc cgctgtggac gacgcgcgcg ccgaaaccct cgacgcgccg
    13321 ctcgaccgcc cggccgatgc ggcccagccc gacgatcccg acggtggcac ggtgcaagtc
    13381 gtgaccgagc aggcgcgccg gctcccaggt cagccactct cccgcgcgca cctcagcgtc
    13441 gcccgcgggg attcgccgca tcacggccag catcagcgcg agcgcgaggt cggcgctcgt
    13501 ctcggtgagg acgtcgggcg tgtgcccgac cggaatgctg cgctcggcgg ccgcagcgag
    13561 atcgatgttg tcggtgccga cggcataggt cgagacggcg cgcagcctgg gcgccgcgtc
    13621 gaagagctcg cggtcgacgg ggtcggtgag catgcagagc agtccctccg cctcggcggc
    13681 gcgctcgtgc agttgctgcg gcgtcggcgg cagccgttcc ggccagacct cgacgtcatg
    13741 ctcggcggcg agccgctcca gcgccgcccc tggcatctcg cgtgttacga agcagcgcgc
    13801 catcgacgcg aacccttcca cgaaaggagc gagcggagca ttcttggggc catggccccg
    13861 tgaatccttc gggcggcggg gtagagccgg tgaagtgccc aaggacaccg gctttccgcg
    13921 gaccgacgcg caatccgact tcacgcgcgc caggcggcgc cgcgcgctcg cgcggctggc
    13981 caaccgcctc aggggcaagc ctggcgcgaa ggtggcgatc ctgccgttcg aggaggtcgt
    14041 cgaggcgctc ggtcgccgcg gagagcgccg gctcggcctg cgctcgatcg cgctcgactc
    14101 gatcgtcggg accgtcgacc gcggacggga gttcgaccgt ggcttccgcc ccacgtctcc
    14161 gcgcccgcgc ggccgcttcg agcggatcgc cacggcaaag cgccgcggcg agccgatgcc
    14221 gcctatccgc gtctaccgga tcgccaacgc ccacttcgtc gaggacggcc accaccgcgt
    14281 cgccgtcgcc cgcgcccagg gccagccgga catcgacgcc tacgtcaccg aggtcgtgac
    14341 cgaggtcggc cacgacctcg acctgcgcag cgtcacgccc gacgagctgc gcgccctgcg
    14401 ctgagcgacg acgtcgctca gccgccggcc gtcagcgacg agccgtcctg cgggtcgaag
    14461 agctggatgt gcgaggcgtc gatgaagagc tccgcctcct cgcctctgct gacctggctc
    14521 tcggccgaca ggcgcgcgac gatctcgccc tcctcgcccg cgcccggaac ctcgccgacg
    14581 ccggagtcct cggcgagctc gcgcagctcc tcggactcga tccgctggtc ggaggtcacg
    14641 cggaagtggg cgtagagctc cgatcccatc gcctcgatca tgtcgatctt cgctcggaag
    14701 gtggcaccgc gaccgcgggc gtcgtcgccg agcagcgcgg cgtcctcgaa gttctcgggc
    14761 cggatgccgg cgatcagctc gcggccggcg gcgtcgccct ggccgatccg ccggcgcaga
    14821 tcctccggca ggcggacgtc gaccatcggc agcttgacgg tgtcgccctc gatcgtcgcc
    14881 ggcatgaagt tcatcgccgg cgagccgatg aaaccggcga cgaagaggtt cttcggccgg
    14941 ttgtagagct ccatcggcgt atcgacctgc tgcagcttgc cggcgcgcat cacggccacc
    15001 cggtcgccaa gcgtcatcgc ctcggtctgg tcatgggtga cgtagatcgt cgtcgtgtcg
    15061 gtgcggttct ggatccgcga gacctcggtg cgcatctgga cgcgcagctt ggcgtcgagg
    15121 ttggagagcg gctcgtccat caggtaggcc gacgggtcac ggacgatcgc gcgccccatc
    15181 gcgacgcgct ggcgctgtcc gccggagagg ttcgccggct ttcggtcgag gtgctcggcg
    15241 aggtcgagga tcttcgccgc ctcctcgacc cgcttgtcga tctcggcctt gtcgacgccg
    15301 tgcagcttga gcgcgaagcc catgttgtcg cgcacggtca tgtgcgggta gagcgcgtag
    15361 ttctggaaga ccatcgcgat gtcgcggtcg cgcggcgcct tctggttgac gacgtcgtcg
    15421 ccgatgaaca gttggccctc gctgatgtcc tcgagccccg cgaccatccg cagcgcggtc
    15481 gacttgccgc atcccgatgg accgaccagg atcatgaact cgccgtcctc gacctcgagg
    15541 ctcatgtcct tgaccgcctc gtagccgtcg ggatagcgct tggtgattcc gtcgaagacg
    15601 atcttggcca tcgcccctcc cttacccgta gtcgcgccgt ttatccccag gccggatgcc
    15661 gagcgaccct cagagcgccg ccagcagctc gttggcggcg gactcgcccg agcggacggc
    15721 cccgtccatg tagcccgacc agacctcggc ggtctccgcg cccgcccagt gcagcgggcc
    15781 gatcggagcg cgcaacgcct cgccatatcc ggtccaggcg ccggtcggca tgtggcagcc
    15841 gtagcagccg cgcgagtact cctcctccgc ccagagccgc tcgacgtagc ggtcggggcg
    15901 agcggcgcgc gcgccgaaca gccgcgtgaa gcagccgatc accgccgcgc ggcggtcgct
    15961 cgcgccgagc cgtccgagct cgcgcgcctg gcggccctcg aggaagccga gcaggacgcc
    16021 gggccggccc gcgggcggcg agttgtcgta ggtcagcttg atcggcccgg tgtcgctgag
    16081 gccctggccc gagagcccgt cgccgcgcca gaacggctcg tcgtagacgg ccatgcactt
    16141 cgcgacggtg ccgagcggca tccgctgggt gagctggtcg cggtgcccgg gaagcggcgg
    16201 gtcatagacg atccgcccgg cgagcgtcgg cgggatcgcg acgatcgccc gccgggcgcg
    16261 gacggtcagg cggtcgtcgc ccgcgccggc cgcgtcgccg acgcggtcgg cgtgaaccgt
    16321 cacgccgtcg ccgccgtgct cgatccggcg gaccgcggcg tcgagcacta cgacgtcgtc
    16381 gccgagccgc tgcgcgagcg cgatcggcac gcgctgggag ccgccgacca agcgatcctg
    16441 ctgagcgccg ccagcggtgt cgagcagccg ctccaacgag ccggccgagt gaatgtagaa
    16501 gagcatgtgc agcagcgaca cgtcctcggg ctgcgcagcc cataccgcct cggtgccgag
    16561 ctgcagcagg tcgcggccgc ccttggtgag gacgttgcgg cgcagccagg tgctcgcggt
    16621 ctgcccgtcc cacacggcgg ccttggacgc gagccacggc cggtcgagcg gcaccgtgcg
    16681 ggccatccgg tcgagccggc gctgcgtgcg caggacgtcg agcaggacga gcgggctgac
    16741 gcgcgggatc gtcccccggt agcggcgcag tgcgccgcgc cactcgatca cgttgtcgcc
    16801 gatggcgtag gtcgggaacg tctccacgtt catctcgtcg gccagggcgg cgatgcggtc
    16861 ctgggtcggc ccgatccact gcccgccgac ctcgacgacg cggtcgcgcg cctcgagcac
    16921 cacgaccgac gctccagcgg cgagcaggtc cctcgccgcg acaagaccgg cgagaccggc
    16981 gccgacgatc gccacgtcgg cctcacgctg ctcgatcatt cggggccgcc tgccaatccc
    17041 cgatcccgtg cccacgtcca ccgtgggcag ccagccctag gccgggagcc gccgcacgcg
    17101 cgccaggccg ttaccgtcgg gagcctggcc gtcgcggccg acgtagccgc ccttggcccc
    17161 gacgatcacg tagacggtgt cgcctccgct gacgttgcgc gtcttgcggt gcgtcgccgc
    17221 cgcgacgcgc gccatgccgc ccgggccgag gcggtgcacc gtcccgtcgc cgaactcgat
    17281 ctcgagctcg ccgctatgga cgaagtacag ctcctcctgc tcgtcgtggt aatggaagtc
    17341 ggactcgatc cccgacggca tcacgatcgc gttgatgccg aactcctcga ctccgagctc
    17401 gcgcctgatc ttgcgaaagc cggggccctc gccgatgtcg tcgaggttgg cgaccgagta
    17461 accctcgccc tgcgtgacgt tgcccatggg tggaccatac cgattgtatg tatccagatc
    17521 cagggtagga gcgggaggtg atcaggatcg gctgctcggg ctggaactac gactcctggc
    17581 gcggggggct ctacccggaa ggcatgggac ctgcgcgctg gctgcgcgag tacgcgcgcc
    17641 acttcgacac ggtcgaggtc aactcgacct tctaccggct cgcctcgcgc aacggcgtcg
    17701 agcgctgggt ggccgagacg cccgacgact tctgcttcgc agtcaagggg agccgctatc
    17761 tgacacacgt caagcgactc gccgacgcgc cggacaagga cttcgtcctc ggcaagggca
    17821 tcgcccgctt ctacgagcgg atcacgccgc tgatccccgc gggcaagctc ggaccggtcc
    17881 tctggcagct gccgcccagc ttcaagcgcg acgacgagcg gctggtcaac ctgctcgagc
    17941 tggtcccgcc cggtcgtcac tgcgtcgagt tccgtcatcc gagctggttc gccgaggacg
    18001 tctaccggct gctgcgcgcg catggagcgg cgctggtgat cgccgaccgg cccgagctga
    18061 cgttccagac ccacgagctg actaccgact ggacgttcgt gcgcttccac tacggccacc
    18121 gcggccggcg cggcaactac tcacgcagcg agctcgagca gtgggcgccg cggatcgccg
    18181 gctggcacga ccgcggcgtc gacgtctacg cgtacttcaa caacgactgg gaggtcttcg
    18241 ccccccgcaa tgcggcgacg ctgaagcggc tgctcgacgt ctgacgccgg gcgctagccg
    18301 cccgccgcct cggccttcgc gaggccgcgc agcgcccaga cgccgaagaa gaccagcatg
    18361 ccggccgcgg cgccgtaggc gagcgcgacg tccgcgggag cgccggagat gaagtcgcgc
    18421 gcggcctcga tcacggcggt gatcgggttg acgctcgcgg cggtcttgac ccacccggtc
    18481 agcaggtcga acgggacgta gaccggggcg atgaagacga cgaggaagac cggtgtctgc
    18541 atcagcggcc ccgcctgcag cgagcggaag cgcagtgcga tccccgcggc ccacatcagc
    18601 gcggtcaggt tcatcagcag ggcgagcgtg tacatcccga cgaggtcggc gacaccgccg
    18661 ccgacctcca ttccgaccgc cagcgcgagg acggtcagca tgatccagat gaacagggcg
    18721 cggccggccg cgacgagcgc gtagccggcg agcagggcgc gccggttcgg cgccgccagc
    18781 atcatccgcc ggccgacgcc accctcgaag tcgcgcgcga tcgcgaagcc ggtgaacacg
    18841 ccgccgaagg cgcttgcctg gagcaggacg aagacgaact ggaacgtcgc gtagttgtcg
    18901 gggaagtcga accccggcac gtcgctgacc gccgacagcc cgccggcgaa ggcgccgaag
    18961 tagaacagcg ggaacagcat cgacgggacg accatcgtcg ggtcgcggaa gaagcggtgc
    19021 agtccgcgcc agccgaccg
//
LOCUS       DATABV010000185        15741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_299671, whole genome shotgun sequence.
ACCESSION   DATABV010000185 DATABV010000000
VERSION     DATABV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299671"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..128)
                     /locus_tag="VHF88_08410"
     tRNA            complement(55..128)
                     /locus_tag="VHF88_08410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Pro,seq:cgg)
     gene            complement(143..1057)
                     /locus_tag="VHF88_08415"
     CDS             complement(143..1057)
                     /locus_tag="VHF88_08415"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX2161828.1"
                     /translation="MRTLVTGATGFVGGRLAQALSGSGVEVRALVRDRKRARPLEQAG
                     IELHEGDVTRAETLRGAGTGADVAYYLVHGMGRGGGKGDFPERERAAALNFARMARAE
                     GVERVIYLGGLGDQPGSEHLRSRHETALTLRREGPPVTYFRASMVVGALSEGYRTLRY
                     LVERLPAMIAPAWLKTPTQPIGIDDAVAYLAAAPQVPESRGREIQIGGPDVLPYGEML
                     DRMAMALGRRPRPKIPFPLLTPWLSSLWIGLVTPVDAGIARPLVEGLATETVVTDPSG
                     AALFEIDPVGFDETLRRALAEDPEARSR"
     gene            1106..2320
                     /locus_tag="VHF88_08420"
     CDS             1106..2320
                     /locus_tag="VHF88_08420"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2161829.1"
                     /translation="MKRAVAIATLCGSLAFAVSACAAESDGPGGATAERRATETTPDG
                     PTGATAARGVRLLPVGRFDQPTYIASPPGDRARRFVTEREGRVVLLRGGRRSTFLDIA
                     DRVQTGGESGLLSIAFHPGYARNRRYFVYYVANDGAIAIFQFRATANGDRTRPGSGRL
                     VMRVPHPRFNHKGGQLQFGHDGMLYAGFGDGGGGGDPDRNAQDLGELLGKIVRIDPRP
                     GGGYRIPRDNPFRGRAGARGEIFAYGLRNPYRFSFDRVTGAMAIGDVGQDAVEEIDFL
                     PAAPGSRRPRGGANLGWSVFEGNRRFRSGSAPGHVRPVIERTHDQGSCSITGGYVIRD
                     RRLGALRGSYVYGDLCDARLRVARLTPRGARRDRALGPRLPQLVSFGEDARGRVYAVS
                     LGGGVFRLAPRR"
     gene            2373..3551
                     /gene="bktB"
                     /locus_tag="VHF88_08425"
     CDS             2373..3551
                     /gene="bktB"
                     /locus_tag="VHF88_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HEX2161830.1"
                     /translation="MTGVVISSAARTAVGSYGKSLAGVPTTDLGATAVTTALVRARTA
                     ADQVDQVVFGNVIHSAPEDMYMARVVGMKAGIPKESPAFTVNRLCGTGVQAIVSGAQA
                     IQTGDADCVVAGGAENMSRGPYWMPDARFGARMGETKMVDPVVGGLTDPFDKVHMGTT
                     AENLAESHSISREAQDEFALESHHRAEAAIAAGKFDDEIVPVEVKVKRDKVEFKQDEH
                     VRPGASIDGFAKLKPVFKQDGTVTAGNASGMNDAGAAVVLMSERKAEELSAPVRAKIL
                     SYAYCGVDPRTMGIGPVPAVKKAMERAGRSLDEVGVIELNEAFAAQALAVMQDLELDP
                     AKVNPNGGAIALGHPISATGAVITAKILSEMEREDHELGLVTLCIGGGQGIALLLART
                     "
     gene            complement(3556..5043)
                     /locus_tag="VHF88_08430"
     CDS             complement(3556..5043)
                     /locus_tag="VHF88_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2161831.1"
                     /translation="MTATLPDALSEAARGFAAGPHRLLIGGERVEAADGATFQTFDPS
                     TGQPIAEVAQAGAEDVDRAVRAARAALEGKWGSLTPAKRSALIYRLAELVEANADELA
                     QLESLDGGKPVGLAKIVDVAKTVEHLRYFAGWPTKIAGQVVPVSQPGVHVYTRREPVG
                     VCGQIIPWNFPLLMAAWKIGPALAAGCTTVLKPAEQTPLTALRLGELALEAGLPEGVL
                     NVVTGDGATGAAIVDHPGIDKLAFTGSTAVGREIGAKAGRALKRVTLELGGKSANVIL
                     PDADLDAAVKGSYQAIYYNTGQACNAGSRLFVQKDQFDDVVAKLAASAGRARVGAGLA
                     EGTQLGPVISAEQEERVRDYIDQGRRDGAELVAGGGTPEGAGDGYFVEPTLFTATSDD
                     ISIAREEIFGPVLVALPYETLEEVAARANDSEYGLAAGVWTRDVGNAHKLAALLDAGN
                     VYVNIWGPSDAAAPFGGVKASGIGREHGLAGLESYLEDKTVWIGL"
     gene            complement(5060..6094)
                     /locus_tag="VHF88_08435"
     CDS             complement(5060..6094)
                     /locus_tag="VHF88_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2161832.1"
                     /translation="MRAVTFQAPGEVRVDEKPTPELGSPDEAIVRVEATGVCGSDLHI
                     YHGRVGVEPGFTIGHEFVGELVAAGGGVSEVAVGDRVLGCYCSACGRCFFCRRGDFHK
                     CDSGRVFGHGEVLGSLQGAQADEVLVPNANLTLRKVPEGMSSDAALFAGDVMGTGFHA
                     VESSGMQPGDTAVVLGLGPVGLCAVQVARACGAARVIAIDTVAERLEMARAFGGEPVH
                     LTEEDPRAAVKAATEGRGADVVIDAVGHPDAFDMACRLARKAGSVSATGVYAERMEVH
                     MGVVWIKALDVHTGHANVIAHVDRVLAMMGAGVLDPSPLVTHHMSLDEAPEAYAAYDR
                     REALKIVMTP"
     gene            6148..6999
                     /locus_tag="VHF88_08440"
     CDS             6148..6999
                     /locus_tag="VHF88_08440"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX2161833.1"
                     /translation="MADVSRAVLITGCSTGIGRATAERLAVNGHTVYATARRPETIAD
                     LEAKGCRTLALDVTDESSMRAAVEAVEDAEGAVGALVNNAGYSQSGAVESLDLDAVRS
                     QFETNVFGLVRMCQLVLPGMRRQRFGRIVNVSSIGGKLVFPGGGAYHATKHAVEAFSD
                     ALRFEVKGFGVDVAIVEPGLIKTEFANAATTALTDGTDRDGPYAEFNRQVGATTVGAY
                     EGPAAKLGGGPETVAKAIEKAIVSRRPKIRYKVTPSAHIVLGQRALFSDRMWDAFVGT
                     QFKRPRA"
     gene            complement(7021..8136)
                     /locus_tag="VHF88_08445"
     CDS             complement(7021..8136)
                     /locus_tag="VHF88_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX2161834.1"
                     /translation="MVRLAVALALVGLTLAAAAPISAQTPSPSPSPGSVPAFDGDAMW
                     IWLMTRTEGGSAARIAARAKRSGIELVILKAGNERTQWTQLNPWLIETLHDAGIRVCG
                     YHFVYGRHPIAEARLSARIARTGADCLVIDAESDYKGRYLEAERYMRTLRRLVGPDYP
                     IGLTSFPYVHYHSTFPYSVFLAPGAADFNVPQMYWRAIGTSVDSIFRTTYMHNRIYGK
                     PIYPLGQVWMQPPPREIRRFRALAAAYGATGVSWWSWQEGTARDFAAVGSPLTRFLAR
                     PRDPGYPLLTTLSRGDIVVQAQRLLLAAGARLRVTGWMDTATRRAIAAFKQRRGIGFG
                     STVTAATWRALLELDPAPVTWRARSYAVPRAPAAAAR"
     gene            complement(8097..8606)
                     /locus_tag="VHF88_08450"
     CDS             complement(8097..8606)
                     /locus_tag="VHF88_08450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2161835.1"
                     /translation="MVTRHVLADGTLVAIRPIVPEDKQELQDGLHRLSDETVQRRFLA
                     PKVRFTKAELRYLTEVDGHDHVALVVESERWPGMIVAVARYVRLNEDPDTAEAAIVVA
                     DALQGLGLGRLLAERLASAATVHGVRRFTAEMYGDNRPAQRLMEVLETRLRDSPAWSG
                     SPSLSPSSG"
     gene            complement(8651..9016)
                     /locus_tag="VHF88_08455"
     CDS             complement(8651..9016)
                     /locus_tag="VHF88_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161836.1"
                     /translation="MSAAGRDGGRDPAARARQALERGASSLGAQLARAASERWQRLPE
                     PRRAKLETLAANVRERALEKRAEPARDVSEIEVRDLRAELARELDRIAGADIAASRGT
                     GELAGEPQSGEGRHRPPPV"
     gene            9069..10007
                     /locus_tag="VHF88_08460"
     CDS             9069..10007
                     /locus_tag="VHF88_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2161837.1"
                     /translation="MRAVTIDDGALTIAERPDPVPGPGEILVAVRAAGINGADILQRK
                     GAYPAPPGAPADIPGLELAGEVVARGPGAQRFAEGDRVMAIVGGGGQAELAVLHERAA
                     MPVPDAVDWPQAGGLAEVFVTAHDAIFGQAGLRPGERLLVHGGAGGVGTAAIQLGRAA
                     GATVTATVRNEELRPEVAKLGAEPIDPTGFEEHGPFDVVLELIGAPNMGANLKALGTG
                     GRVVVIGVGAGFKAEVNLLAVMGKRATIGGSTLRARPLEQKALAMRRVEAEVLPLFES
                     GTLTVPVAASFPLADADAAYERFTAGGKLGKIVLIT"
     gene            complement(10004..10606)
                     /locus_tag="VHF88_08465"
     CDS             complement(10004..10606)
                     /locus_tag="VHF88_08465"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2161838.1"
                     /translation="MATRTTKAEQGEASRATLVRAARELFTERGYGAVGTEEIVRRAK
                     LTRGALYHHFEDKKDLFRAVHEQIEGEVMAAIGAQMAQADGDPYSLLVVGTRAYLDVC
                     TDPAVTRVTLVDAPSVLGWEEWREIDMRHGLGIVIAGLQGGMDAGVLRRQPVRPLAHL
                     MLGAMGEAGMMIANAPDPEAARAELEPALLGLLDGLRASG"
     gene            10694..11590
                     /locus_tag="VHF88_08470"
     CDS             10694..11590
                     /locus_tag="VHF88_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2161839.1"
                     /translation="MSTTTDRAGWRSPALGEPKTIELPGRRMRYHDVGAGPTLVFVHG
                     LLVNANLWRKVIERLAPDFRCVVLDLPLGSHTLPLAAGTPNAVPDVAASIADTIEALD
                     LDDVTLVGNDSGGALSQIVVTTRPERIGRLVLTSCDYRDNFPPKLFSYFKPAAAVPGL
                     MWALLQPMRLHLPRRSPLAFGWLTKRPVDRVAEDSYMLPVLSDAAIRADVRAFIKGAD
                     IAQTNAAADRLPQFDRPALIAWSADDRVFPVADAHKLAADLPNSRVELIEDAYTFSME
                     DNPARLSELIAGFVREPVGGAA"
     gene            11587..11952
                     /locus_tag="VHF88_08475"
     CDS             11587..11952
                     /locus_tag="VHF88_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HEX2161840.1"
                     /translation="MSDVPILAVAAGFGAMGVAALVRPAQVLAQFGVAVDTANGRNEV
                     RAVYGGFGLAVMAMLVVAALGDPATADGIVVAVAVALLGMAAGRLLSALRERPTGLYP
                     VWVYFAIEVAAATALLAAI"
     gene            complement(11954..13120)
                     /locus_tag="VHF88_08480"
     CDS             complement(11954..13120)
                     /locus_tag="VHF88_08480"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX2161841.1"
                     /translation="MPDAVIVDAVRTPIGRAFKGSLAPLRPDNTGAFIVDQLLERNPG
                     VDPTSVEEVVAGCGLPQGKQAFNIGRLIALLSEKLPQEVNGSTVSRYCASGLDAIRIA
                     SNNVKAGQGDVYIAAGVEFVSQYNERQEAAGLADQNEKLQGKEPGQPDVYIQMGETAV
                     NVAKKYGVGREDMDKYAQRSQELAVGSQENGFFDREIVPVTLPDGTVVSKDDGPRASS
                     SLEKLASLPEAFPGGGGVTAGNSCPLNDGAAAALIMSDEKARELGLKPRARIITAATW
                     GNEPEYMGVAPIGAIKKALERAGMTIDDVDTVELNEAFAAQVIPIMDECGIPLEKMNP
                     HGGAIALGHPFGQTGVRIMTTLLNGLESDDKTIGLETMCVAGGQGEAMIVERLR"
     gene            complement(13199..13966)
                     /locus_tag="VHF88_08485"
     CDS             complement(13199..13966)
                     /locus_tag="VHF88_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2161842.1"
                     /translation="MEINGIAAFVSGGASGLGEATVRALIARGARVAIADVNDDKANA
                     LAAELGDAAIAVHCDVTSEQETQAAVEAAVEAFGELRLAVGCAGVGWAEKTVSKRGAH
                     QLLPFETVVRVNLIGMFNVLRLTAAAMNENEPGTDGERGAIVMTASVAAFDGQIGQIS
                     YSASKAGIAGMTLPAARDLSSRGIRVCTIAPGLFDTPLLAALPEEARVALGAGVPFPQ
                     RLGQPSEYAQLALSIAENPMLNGETIRLDGALRMPPK"
     gene            complement(14015..14995)
                     /gene="egtD"
                     /locus_tag="VHF88_08490"
     CDS             complement(14015..14995)
                     /gene="egtD"
                     /locus_tag="VHF88_08490"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX2161843.1"
                     /translation="MTAGPLTAAITIERCDPTDAPQSLAAEVRAGLGRALKELPPKCF
                     YDERGSALFDMITELPEYYPTRCEREIIERHADEIVARATELVELGSGTASKTRALLD
                     AMAAAESLRRYVPFDVDPTVVETCAEELTARYPGLLVHGVVGDFGRDLGRIPAGGDRL
                     FAFLGGTIGNLYPAERARFLERLAALMAAGDRLLIGVDLVKDRAVLEAAYNDSAGVTA
                     EFNRNVLRVLNARLDADFDPSAFEHVAFFDEQHSWIEMRLRAQGAQTVRIEAAGLELE
                     LADGEELRTEISAKFTRRGVERELSGAGMRLDAFHTDAGGLFGLAVATLP"
     gene            complement(15012..>15741)
                     /locus_tag="VHF88_08495"
     CDS             complement(15012..>15741)
                     /locus_tag="VHF88_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX2161844.1"
                     /translation="QGPADGPAAFSACVIEMGDKGDEIAYDNERPRHRVELGAYAIDR
                     APVSNGEYAQFVADGGYARPELWSEAGWAWREGERAERPLYWTGDGDERRFDRLEPLA
                     PHLPVMHVSWFEAEAYARWAGGRLPTEAEWEHAAVALDEGRRGNLDQLDFAPGHAGRF
                     VGDCWEWTASELRGYPGFRAFPYPEYSQVFFDGGYRVLRGSSWATRPTVARPTFRNWD
                     HPQRRQIFAGFRCGYDVAADRSNR"
BASE COUNT         2149 a         5738 c         5628 g         2226 t
ORIGIN      
        1 gccggcgccg ccatcgacag gcgggtgtag aagcggtcgc tgggcatggc gaaatcgggc
       61 tgccgagatt tgaactcggg acctctccga cccgaacgga gcgcgctacc agactgcgcc
      121 acagcccgtg accgctcgaa ggctatcgcg agcgcgcctc cgggtcctcg gccagcgccc
      181 ggcgcagcgt ctcgtcgaag ccgaccgggt cgatctcgaa gagcgcggcg cccgacgggt
      241 cggtgacgac cgtctcggtc gcgagcccct cgaccagcgg acgggcgatg cccgcgtcga
      301 ccggcgtcac caggccgatc cagagcgatg acagccatgg agtgagcagc gggaacggga
      361 tcttcgggcg cggccggcgg ccgagcgcca tcgccatccg gtcgagcatc tcaccgtagg
      421 gcaggacgtc cggaccgccg atctggatct cgcgaccgcg ggattcgggc acctgcggcg
      481 ccgctgccag gtaggcgacg gcgtcgtcga tgccgatcgg ctgtgtcggc gtcttcagcc
      541 aggccggcgc gatcatcgcc ggcagccgct cgacgaggta gcgcagcgtc cggtagccct
      601 cgctgagcgc gccgacgacc atgctggcgc ggaagtaggt caccggcggg ccctcgcggc
      661 gaagcgtcaa agcggtctcg tgacggctgc gcaggtgctc ggagccgggc tggtcgccga
      721 gcccgccgag gtagatcacg cgctcgactc cctcggcgcg ggccatccgc gcgaagttca
      781 gcgccgccgc ccgctcgcgc tcggggaagt cgcccttgcc gcccccgcgg cccatgccgt
      841 ggaccaggta gtaggcgacg tcggctcccg tgccggcgcc gcgcaacgtc tcagcccggg
      901 tgacgtcgcc ctcgtggagc tcgatccccg cctgctcgag tggacgggcg cgcttcctgt
      961 cgcggacgag agcgcgcacc tcgacgccgc tgccactcag ggcttgcgcc agtcggccgc
     1021 cgacgaatcc cgtcgctccg gttacgagcg tccgcatcgt ggggacttac ccagcgttgc
     1081 cggcccaatc ctcatacttg gcgacgtgaa gcgcgcggtt gccatagcga cgctctgcgg
     1141 gtcgctcgcc ttcgcggtca gcgcctgcgc agctgagagc gacgggcccg gcggcgcgac
     1201 ggccgagcga cgtgccactg agacgacgcc ggacggaccg acgggcgcga ccgccgcgcg
     1261 cggcgtgcgc ctgctgccgg tcgggaggtt cgaccagccg acctacatcg cgtcgccgcc
     1321 cggcgatcgc gcccgccgct tcgtcaccga gcgcgaggga cgggtcgtcc tgctgcgcgg
     1381 gggccgccgc tcgaccttcc tcgacatcgc cgaccgggtc cagaccggcg gcgagagcgg
     1441 gctgctgtcg atcgccttcc accccggcta cgcgcgcaac cgacgctact tcgtctacta
     1501 cgtcgccaac gacggcgcga tcgcgatctt ccagttccgc gccaccgcga acggtgaccg
     1561 cacgcgtccg ggctccggcc gcctcgtgat gcgcgtgccg catccgcgct tcaaccacaa
     1621 gggcggtcag ctccagttcg gccacgacgg gatgctctac gccggcttcg gcgacggtgg
     1681 cggcggcgga gaccccgacc gcaacgcgca ggacctcggc gagctgctcg gcaagatcgt
     1741 ccggatcgat ccccggccgg gcggcggcta tcgaatcccg cgcgacaacc cgtttcgcgg
     1801 ccgcgccggc gcgcgcggcg agatcttcgc ctacggcctg cgcaacccgt accgcttctc
     1861 gttcgaccgc gttaccggcg ccatggcgat cggcgacgtc ggtcaggacg cggtcgagga
     1921 gatcgacttc ctgcccgcgg cgcctggcag ccggcgaccg cgcggcggcg ccaacctcgg
     1981 ctggtcggtc ttcgagggca accgccgctt ccgctccggc agcgcgccag gccacgtccg
     2041 gcccgtcatc gagcgcaccc acgaccaggg cagctgctcg atcaccggtg gctacgtgat
     2101 ccgtgaccgc cgcctcggcg cgctgcgcgg cagctacgtc tacggagacc tctgcgacgc
     2161 tcgcctgcgc gtcgcgcgcc tgacgccccg gggcgcgcgc cgcgaccgcg ccctcgggcc
     2221 gcgcctgccg cagctcgtgt cgttcggcga ggacgcgcgc ggccgggtct acgccgtctc
     2281 gctcgggggc ggcgtcttcc gcctcgcccc gcgccgctag cgggcgccgg tacccttcgc
     2341 ggtccgtagc ccaccccagg aggaccgttt cagtgaccgg agtcgtgatc tcgtccgccg
     2401 cgcgcaccgc cgtcggctcg tacggaaaga gcctcgccgg cgtgccgacc accgacctcg
     2461 gcgcgaccgc ggtcaccacg gcgctcgtgc gcgcccggac ggcggccgac caggtcgacc
     2521 aggtcgtctt cggcaacgtc atccactcgg cgccggagga catgtacatg gcccgcgtcg
     2581 tcgggatgaa ggcggggatc ccgaaggagt cgcccgcctt caccgtcaac cgcctctgcg
     2641 gcaccggcgt ccaggcgatc gtctcaggcg cccaggcgat ccagaccggc gacgcggact
     2701 gcgtcgtcgc cggcggcgcc gagaacatga gccgcggccc gtactggatg ccggacgctc
     2761 gcttcggcgc ccggatgggc gagacgaaga tggtcgaccc ggtcgtcggc ggcctgaccg
     2821 accccttcga caaggtccac atgggcacca ccgccgagaa cctcgccgag tcgcactcga
     2881 tctcgcgcga ggcgcaggac gagttcgcgc tcgagtccca ccaccgcgct gaggccgcga
     2941 tcgccgcggg caagttcgac gacgagatcg ttcccgtcga ggtcaaggtc aagcgcgaca
     3001 aggtcgagtt caagcaggac gagcacgtgc ggccgggcgc ctcgatcgac ggcttcgcga
     3061 agctcaagcc ggtcttcaag caggacggca ccgtgacggc cggcaacgcc tcgggcatga
     3121 acgacgccgg cgccgcggtc gtgctgatgt cggagcgcaa ggcggaggag ctcagcgccc
     3181 cggtgcgcgc gaagatcctc tcctacgcct actgcggcgt cgacccgcgc acgatgggca
     3241 tcggcccggt gccggcggtc aagaaggcga tggagcgcgc cggccgctcg ctcgacgagg
     3301 tcggcgtgat cgagctcaac gaggcgttcg ccgcccaggc gctcgcggtc atgcaggacc
     3361 tcgagctcga tcccgccaag gtcaacccga acggcggcgc gatcgcgctc ggccacccga
     3421 tctcggccac cggcgccgtg atcaccgcca agatcctctc cgagatggag cgcgaggacc
     3481 acgagctcgg cctcgtcacg ctctgcatcg gcggcggcca gggcatcgcc ctgctcctag
     3541 cgcgaaccta gcgagctaga ggccgatcca gacggtcttg tcctcgaggt aggactcgag
     3601 gccggcgagg ccgtgctcgc ggccgatgcc ggaggccttg acgccgccga agggggcggc
     3661 ggcgtcgctc ggcccccaga tgttcacgta gacgttgccg gcgtcgagca gtgcggcgag
     3721 cttgtgggcg ttgccgacgt cgcgcgtcca gacgccggcg gcgaggccgt actcgctgtc
     3781 gttggcgcgc gcggcgacct cctcgagggt ctcgtagggg agggcgacga gcaccggccc
     3841 gaagatctcc tcgcgggcga tcgagatgtc gtcggaggtc gcggtgaaga gcgtcggctc
     3901 gacgaagtag ccgtcaccgg cgccctcggg cgtgccgccg ccggcgacga gctcggcgcc
     3961 gtcgcggcgg ccctggtcga tgtagtcgcg cacgcgctcc tcctgctcgg ctgagatcac
     4021 cgggccgagc tgcgtgccct ctgcgaggcc ggcgccgacg cgcgccctgc cggcggaggc
     4081 ggcgagcttg gcgacgacgt cgtcgaactg gtccttctgg acgaacagcc gcgagccggc
     4141 gttgcaggcc tggccggtgt tgtagtagat cgcctggtag ctgcccttga cggcggcgtc
     4201 gaggtcggcg tcgggcagga tcacgttggc cgacttgccg cccagctcga gcgtgacgcg
     4261 cttcagcgca cggccggcct tggcgccgat ctcgcggccg accgccgtcg agccggtgaa
     4321 ggcgagcttg tcgatgccgg ggtggtcgac gatcgccgcg ccggtcgcgc cgtcgccggt
     4381 gacgacgttc agcacgccct cgggcaagcc cgcctcgagc gccagctcgc cgagccgcag
     4441 cgccgtcagc ggcgtctgct cggccggctt gagcacggtc gtgcagccgg cggcgagcgc
     4501 cggcccgatc ttccaggcgg ccatcagcag cgggaagttc cacgggatga tctggccgca
     4561 gacgccgacc ggctcgcggc gcgtgtagac gtgcacgccg ggctgggaga cggggaccac
     4621 ctggccggcg atcttcgtcg gccagccggc gaagtagcgc aggtgctcga cggtcttggc
     4681 gacgtcgacg atcttcgcga ggccgaccgg cttgccaccg tcgagcgact cgagctgcgc
     4741 gagctcgtcg gcgttggcct cgaccagctc ggcgagcctg tagatcagcg cgctgcgctt
     4801 cgccggcgtc agcgatcccc acttgccctc gagcgccgct cgagcggcgc gcacagcccg
     4861 gtcgacgtcc tccgcgccgg cctgggcgac ctcggcgatc ggctgtccgg tcgacgggtc
     4921 gaaggtctga aaagtcgcgc cgtcggccgc ctcgacgcgc tcgccgccga tcagcaggcg
     4981 gtgcgggccc gcggcgaacc cgcgagcggc ctcggagaga gcgtctggaa gtgtcgcggt
     5041 catcgtcggt ctcgcctcct cacggcgtca tcacgatctt cagcgcctcg cggcggtcgt
     5101 atgcggcgta ggcctcgggc gcctcgtcga gcgacatgtg gtgggtgacc agcggcgacg
     5161 ggtcgagcac gccggcgccc atcatcgcca gcacgcggtc gacgtgggcg atcacgttcg
     5221 cgtggccggt gtggacgtcg agcgccttga tccagacgac gcccatgtgg acctccatcc
     5281 gctcggcgta gacgccggtc gcggacacgc tgccggcctt gcgcgcgagc cggcaggcca
     5341 tgtcgaaggc gtcggggtgg ccgacggcgt cgatcactac gtcggcgccg cggccctcgg
     5401 tcgccgcctt gaccgccgcg cgggggtcct cctcggtcag gtggacgggc tcgccgccga
     5461 acgcgcgcgc catctcgagc cgctcggcga cggtgtcgat cgcgatcacg cgcgcggcac
     5521 cgcaggcgcg ggcgacctgg acggcgcaca ggccgaccgg tccgagcccg agcacgaccg
     5581 cggtgtcgcc tggctgcatc cccgacgact cgaccgcgtg gaagccggtc cccatcacgt
     5641 cgccggcgaa cagcgcggcg tcgctcgaca tcccctcggg gaccttgcgc agcgtcaggt
     5701 tcgcgttggg caccagcacc tcgtcggcct gggcgccctg cagcgagccg agcacctcgc
     5761 cgtggccgaa gacgcggccg gagtcgcact tgtggaagtc gccacggcgg cagaagaagc
     5821 agcgcccgca ggccgagcag tagcagccga gcacgcgatc gccgaccgcg acctcgctga
     5881 cgccgccgcc ggccgcgacc agctcgccga cgaactcgtg gccgatcgtg aagcccggct
     5941 ctaccccgac ccggccgtgg tagatgtgga ggtcggatcc gcagaccccg gtcgcctcga
     6001 cgcggacgat cgcctcgtcc ggcgagccga gctccggcgt cggcttctcg tcgacccgga
     6061 cctcgccggg cgcctggaat gtgacggcgc gcatcgcggg caacggtatc gtccgctcgc
     6121 atcaggggac tcgcgagggg agagacgatg gccgacgtgt cgagggcagt gctgatcacg
     6181 gggtgctcga ccgggatcgg tcgagcgaca gctgagcgac tcgccgtcaa cggccacacc
     6241 gtctacgcga ccgcgcggcg gcccgagacg atcgccgacc tcgaggccaa gggctgccgg
     6301 acgctcgcgc tcgacgtcac cgacgagagc tcgatgcggg cggctgtcga ggcggtcgag
     6361 gacgccgagg gcgccgtcgg cgcgctggtc aacaacgccg gctacagcca gtcgggagcg
     6421 gtcgagtcgc tcgacctcga cgccgtccgc agccagttcg agaccaacgt cttcggcctc
     6481 gtccggatgt gccagctggt cctgcccggc atgcgccgcc agcgcttcgg gcggatcgtc
     6541 aacgtcagct cgatcggcgg caagctggtc ttcccgggcg gcggcgccta ccacgcgacc
     6601 aagcacgcgg tcgaggcgtt cagcgacgcg ctgcgcttcg aggtcaaggg cttcggcgtc
     6661 gacgtcgcga tcgtcgagcc cgggctgatc aagaccgagt tcgccaacgc cgcgacgacc
     6721 gcgctgaccg acggcaccga tcgcgacggc ccctacgccg agttcaaccg gcaggtcggc
     6781 gcgacgaccg tcggcgccta cgaggggccg gcggcgaagc tcggcggcgg tcccgagacc
     6841 gtcgccaagg cgatcgagaa ggcgatcgtc agcaggcggc cgaagatccg ctacaaggtc
     6901 acgccgtcgg cgcacatcgt gctcggccag cgcgcgctgt tcagcgaccg catgtgggac
     6961 gccttcgtcg gcacgcagtt caagcggccg cgcgcgtagc cgagtcccgt gccggcgacg
     7021 tcagcgtgct gccgctgccg gcgcgcgcgg gacggcgtac gaccgcgccc gccaggtcac
     7081 cggagcggga tcgagctcga gcagggcgcg ccaagtagcg gcggtgacgg tgctgccgaa
     7141 gccgatcccg cgccgctgct tgaacgcggc aatcgcacga cgggtcgccg tgtccatcca
     7201 gcccgtgacc ctcagccggg cgcccgccgc gagcagcagc cgctgggcct ggacgacgat
     7261 gtcgccgcgc gagagcgtcg tcagcagcgg atagccgggg tcgcgcgggc gcgccaggaa
     7321 gcgcgtcagc ggggagccga cggcggcgaa gtcgcgcgcg gtcccctcct gccacgacca
     7381 ccagctcacg cccgtcgcgc cgtaggcggc agccaaggcg cggaagcggc ggatctcgcg
     7441 cggcggcggc tgcatccaga cctgcccgag cggatagatc ggcttgccgt agatccggtt
     7501 gtgcatgtag gtcgtacgga agatcgagtc gaccgacgtg ccgatcgcgc gccagtacat
     7561 ctgcggcacg ttgaagtcgg ccgcgcccgg cgccaggaag acggagtagg gaaaggtcga
     7621 gtggtagtgg acgtagggga agctggtcag cccgatcggg tagtccggcc cgaccagccg
     7681 gcgcagcgtt cgcatgtagc gctccgcctc gaggtagcgg cccttgtagt cgctctcggc
     7741 gtcgatcacc aggcagtccg cgccggtcct cgcgatccgc gcgctcagcc gcgcctcggc
     7801 gatcgggtgg cggccgtaga cgaagtggta gccgcagacc ctgatccccg cgtcgtgcag
     7861 cgtctcgatc agccaggggt tgagctgggt ccactgggtc cgctcgttgc ccgccttgag
     7921 gatcaccagc tcgatcccac tgcgcttcgc acgcgctgcg atccgcgccg cgctgccgcc
     7981 ctcggtccgg gtcatcagcc agatccacat cgcgtcgccg tcgaacgccg ggacggagcc
     8041 cggcgacggg gacggcgacg gcgtctgggc cgagatgggg gcggccgccg cgagggttag
     8101 cccgacgagg gcgagagcga cggcgagccg gaccacgccg gggagtctcg cagccgcgtc
     8161 tcgagcactt ccatcagccg ctgggcgggg cggttgtctc catacatctc ggcggtgaag
     8221 cggcgcacgc cgtggaccgt cgccgcgctg gccagccgct cggcgagcag ccggcctagt
     8281 cccagcccct gcagcgcgtc ggcgacgacg atcgccgcct cggctgtgtc ggggtcctcg
     8341 ttcagccgga cgtagcgagc gaccgcgacg atcatgccgg gccagcgctc ggactcgacg
     8401 acgagcgcca cgtggtcgtg gccgtcgacc tcggtcaggt agcgcagctc ggccttggtg
     8461 aaacggacct taggcgccag gaaccgccgc tgcacggtct cgtccgagag gcggtggagg
     8521 ccgtcctgga gctcctgctt gtcctccggc acgatcgggc ggatcgcgac cagcgtccca
     8581 tcggccaaga cgtgccgtgt gaccatggtt acgagtgtcg caccatcgag gcgcgcttcc
     8641 gtgtcggccg tcacacaggc ggtgggcggt ggcggccttc gcccgactgc ggctcgcccg
     8701 ccagctcgcc cgtaccgcgc gacgccgcga tgtcggctcc ggcgatccgg tccagctcgc
     8761 gcgcgagctc ggcgcgcagg tcgcgcacct cgatctcgct gacgtcgcga gccggctcgg
     8821 cgcgcttctc gagtgcccgc tccctgacgt tcgccgccag cgtctcgagc ttcgcccggc
     8881 ggggctcggg cagccgttgc cagcgctcgc tcgcggcacg ggcgagctgg gcgcccaagc
     8941 tgctcgcgcc gcgctcgagc gcctgccgcg cacgcgcggc gggatcccga ccgccatctc
     9001 tcccggctgc tgacatcgcg ggcatggtcg cgatcggggt ggacggacag cgggataggc
     9061 tcccggccat gcgagcggtg acgatcgacg acggcgccct gacgatcgcg gagcggccgg
     9121 atcccgttcc cggcccgggc gagatcctcg ttgcggtacg cgccgccggg atcaacggcg
     9181 ctgacatcct ccagcgcaag ggcgcctatc ccgcgccccc cggcgcgccg gcggacatcc
     9241 ccgggctcga gctggccggc gaggtcgtcg cgcgcgggcc gggggcccag cggttcgccg
     9301 agggcgaccg cgtgatggcg atcgtcggcg gcggcggcca ggccgagctc gccgtgttgc
     9361 acgagcgcgc cgcgatgccg gtgcccgacg cggtcgactg gccgcaggcc ggcgggctcg
     9421 ccgaggtctt cgtcacagcg cacgatgcga tcttcggcca ggccggcctg cgtcccggcg
     9481 agcggctgct cgtccacggc ggcgccggcg gcgtcgggac ggcagcgatc caactcggcc
     9541 gggcggcggg ggcgacggtg accgcaacgg tccgcaacga ggagctgcgg cccgaggtcg
     9601 ccaagctcgg cgccgagccg atcgacccca cgggcttcga ggagcacggc cccttcgacg
     9661 tcgtgctcga gctgatcggc gcgccgaaca tgggcgccaa cctgaaggcc ctcgggaccg
     9721 ggggccgggt cgtcgtgatc ggcgtcggcg ccggcttcaa ggccgaggtc aacctgctcg
     9781 cggtgatggg caagcgcgcg acgatcggcg gctctacttt gcgcgcccgc ccgctcgagc
     9841 agaaggcgct cgcgatgcgc cgcgtcgagg ccgaggtcct gccgctcttc gagtccggca
     9901 cgctgaccgt cccggtcgcc gcaagcttcc cgctcgcaga cgccgacgcc gcctacgagc
     9961 gcttcaccgc cggtggcaag ctcggcaaga tagtgctgat cacctagccg cttgccctga
    10021 gcccgtcgag caggccgagc agggccggtt cgagctcggc gcgcgccgcc tcgggatcgg
    10081 gcgcgttggc gatcatcatc ccggcctcgc ccatcgcgcc gagcatcagg tgggcgagtg
    10141 ggcggaccgg ctggcggcgc agcacgccgg cgtccatccc gccctgcagg cccgcgatca
    10201 caatgccgag gccgtggcgc atgtcgatct cgcgccactc ctcccagccg agcaccgacg
    10261 gcgcgtcgac cagcgtcacg cgggtgaccg ccgggtcggt gcagacgtcg aggtaggcac
    10321 gcgtgccgac gaccagcagc gagtacgggt cgccgtcggc ctgggccatc tgggccccga
    10381 tcgccgccat gacctcgccc tcgatctgct cgtggacagc gcggaacaga tccttcttgt
    10441 cctcgaagtg gtggtagagc gcaccgcgcg tgagcttcgc ccgccggacg atctcctcgg
    10501 tgccgacggc gccgtagcca cgctcggtga acagctcacg tgcggcccgc acgagtgtcg
    10561 cgcggctcgc ctcgccctgc tcggccttcg tcgtcctcgt cgccacggtc taatcgaccc
    10621 taccgcgttg cactacatac atacagcctg tatgttgatc gcgaacagca acgcgggcga
    10681 agggagcacg gcgatgagca ctactaccga ccgagcggga tggcgctcac ccgccctggg
    10741 tgagccgaag acgatcgagc tgcccggcag gcggatgcgc taccacgacg tcggcgcggg
    10801 accgacgctc gtcttcgtcc acgggctgct ggtcaacgcc aacctgtggc gcaaggtcat
    10861 cgagcggctc gcgccggact tccgctgcgt cgtgctcgac ctgccgctcg gctcgcacac
    10921 gctgccgctc gccgcgggga cgccgaacgc cgttccggac gtagccgcct cgatcgccga
    10981 cacgatcgag gcgctcgacc tagacgacgt gacgctcgtc ggcaacgaca gcggcggcgc
    11041 gctgtcgcag atcgtcgtga cgacgcggcc ggagcggatc ggccggctcg tactcacctc
    11101 ctgtgactac cgcgacaact tccctccgaa gctcttctcg tacttcaagc cagcggcggc
    11161 ggttcccggc ctgatgtggg cgctgctgca gccgatgcga ctgcacctcc cgcgccgctc
    11221 gccgctcgcc ttcgggtggc tgaccaagcg gccggtcgat cgggtcgccg aggactccta
    11281 catgcttccc gtcctctccg acgccgcgat tcgcgccgac gttcgcgcct tcatcaaggg
    11341 cgccgacatc gcgcagacga acgccgccgc cgaccggctg ccgcagttcg acaggcctgc
    11401 gctgatcgcc tggtcggccg acgatcgggt cttcccggtc gccgacgccc acaagctcgc
    11461 cgccgacctc ccgaacagcc gtgtcgagct gatcgaagac gcctacacct tctcgatgga
    11521 ggacaacccc gcacgactgt ccgagctgat cgccggcttc gtccgcgagc ccgtaggggg
    11581 cgccgcatga gcgacgtgcc gatcctcgcc gtcgccgccg gcttcggcgc gatgggagtc
    11641 gccgcgctcg tccggcccgc tcaggtgctc gcccagttcg gagtcgcggt cgatacggcc
    11701 aatggccgca acgaggtccg cgcggtctac ggaggcttcg gcctcgccgt catggcgatg
    11761 ctggtcgtcg ccgcgctcgg cgatcccgcg accgcggacg gcatcgtcgt cgcggtcgcc
    11821 gtcgccctgc tcggaatggc ggccggccgg ctgctcagcg cgctgcgcga gcggccgacc
    11881 ggcctctatc ccgtctgggt ctacttcgcg atcgaggtcg ccgccgcgac ggccctcctc
    11941 gcagcgatct gagttaccgc aggcgctcga cgatcatcgc ctcgccctgg ccgccggcga
    12001 cgcacatcgt ctcgaggccg atcgtcttgt cgtcggactc gaggccgttg agcagcgtgg
    12061 tcatgatccg cacgccggtc tggccgaacg ggtggccgag tgcgatcgcc ccgccgtgcg
    12121 ggttcatctt ctcgagcggg atgccgcact cgtccatgat cgggatcacc tgggcggcga
    12181 acgcctcgtt gagctcgacg gtgtcgacgt cgtcgatcgt catgccggca cgctcgagcg
    12241 ccttcttgat cgcgccgatc ggggcgacgc ccatgtactc gggctcattg ccccaggtcg
    12301 ccgcggtgat gatgcgggca cgcggcttca ggccgagctc gcgcgccttc tcgtccgaca
    12361 tgatcagcgc ggccgccgcg ccgtcgttca gcgggcagga gttgcccgcg gtcacgccgc
    12421 cgccaccggg gaacgcctcg ggcagcgacg ccagcttctc gagcgacgac gaggcgcgcg
    12481 ggccgtcgtc cttggagacc accgtgccgt ccggcagcgt caccgggacg atctcgcggt
    12541 cgaagaagcc gttctcctgc gagccgaccg ccagctcctg cgagcgctga gcgtacttgt
    12601 ccatgtcctc gcggccgacg ccgtacttct tggcgacgtt gacggccgtc tcgcccatct
    12661 ggatgtagac gtcgggctgg cccggctcct tgccctgcag cttctcgttc tggtcggcga
    12721 ggccggcggc ctcctggcgc tcgttgtact gggagacgaa ctcgacgccc gcggcgatgt
    12781 agacgtcgcc ctggcccgcc ttgacgttgt tggaggcgat ccggatggcg tcgaggccag
    12841 acgcgcagta gcgcgacacg gtcgagccgt tgacctcctg cggcagcttc tcggacagca
    12901 gtgcgatcag ccggccgatg ttgaaggcct gcttgccctg cgggagaccg cagccggcga
    12961 cgacctcctc gaccgaggtc gggtcgacgc ccgggttgcg ctcgaggagc tggtcgacga
    13021 tgaaggcgcc ggtgttgtcg gggcggagcg gggccagcga ccccttgaag gcgcgtccga
    13081 tcggggtgcg gactgcgtcg acgatgactg cgtcaggcat tgcggggcgt ctcccttgtg
    13141 tggttctgga aaattgctcg agctgggccg aagactctag ggcgcgactg gcagccgatc
    13201 acttgggcgg catccgcagc gcgccgtcga ggcggatcgt ctctccgttg agcatcgggt
    13261 tctcggcgat cgagagtgcc agctgggcgt actcggacgg ctggccgagc cgctgcggga
    13321 atgggacgcc ggcgccgagc gcgacgcgcg cctcctcggg cagcgccgct agcagcggcg
    13381 tgtcgaacag gcccggcgcg atcgtgcaga cccggatgcc gcggctcgac aggtcacgcg
    13441 ccgcgggcag cgtcatgccg gcgatgccgg ctttggaggc ggagtaggag atctggccga
    13501 tctggccgtc gaatgcggcg accgaggcgg tcatcacgat cgcgccgcgc tcgccgtccg
    13561 taccgggctc gttctcgttc atcgccgcgg ccgtcaggcg caggacgttg aacatcccga
    13621 tcaggttgac ccggaccacg gtctcgaatg gcagcagctg atgggcgccg cgcttcgaca
    13681 ccgtcttctc cgcccagccg acgcccgcgc agccgaccgc caggcgcagc tcgccgaacg
    13741 cctcgaccgc ggcctcgacg gccgcctggg tctcctgctc ggaggtgacg tcgcagtgga
    13801 cggcgatcgc ggcgtcgccg agctcggcgg cgagcgcgtt cgccttgtcg tcgttgacgt
    13861 cggcgatcgc gacgcgcgcg ccgcgcgcga tcagcgcgcg cacggtcgcc tcaccgaggc
    13921 ccgaggcccc gcccgacacg aatgcagcga ttccgttgat ctccatggcg tcgaacccta
    13981 ttgactggcc aatcagccag gcatctcggg cgactcaggg gagcgtcgcg acggcgaggc
    14041 cgaagaggcc gccggcgtcg gtgtgaaagg cgtctaggcg catcccggcg ccgctcagct
    14101 cgcgctcgac gccgcggcgg gtgaacttcg cgctgatctc ggttcgcagc tcctcgccgt
    14161 ccgcgagctc gagctcgagc cccgccgcct cgatccgcac cgtctgcgcg ccctgcgcgc
    14221 gcagccgcat ctcgatccag gagtgctgct cgtcgaagaa cgcgacgtgc tcgaaggcgc
    14281 tcgggtcgaa gtcggcgtcg agcctcgcgt tgagcacgcg caggacgttg cggttgaact
    14341 cggctgtcac gccggcgctg tcgttgtagg cggcctccag caccgcgcgg tccttgacca
    14401 ggtcgacgcc gatcagcagg cggtcgccgg cagccatcag tgcggcgagc cgctccagga
    14461 aacgtgcccg ctctgccggg tagaggttgc cgatcgtccc gccgaggaac gcgaacaggc
    14521 ggtcgccgcc agccgggatg cgtccgaggt cgcggccgaa gtcgccgacg accccgtgca
    14581 cgagcagccc tggatagcgc gccgtcagct cctcggcgca cgtctcgacg accgtcgggt
    14641 cgacgtcgaa gggtacgtag cgccgcaggg actcggccgc ggccatcgcg tcgagcagcg
    14701 cgcgcgtctt ggaggcggtc cccgagccca gctcgaccag ctcggtggcg cgggcaacga
    14761 tctcgtccgc atggcgctcg atgatctccc gctcgcaacg cgtcgggtag tactcgggca
    14821 gctcggtgat catgtcgaag agcgccgagc cgcgctcgtc gtagaagcac ttgggcggca
    14881 gctccttcag cgcccgtccg agaccggcgc gcacctccgc ggcgagcgac tgcggcgcat
    14941 cggtgggatc gcagcgctcg atcgtgatcg ccgcggtcag cgggcctgcg gtcatcggac
    15001 tccctccccg gtcatcgatt gctccgatcc gccgcgacat cgtatccgca gcggaagccg
    15061 gcgaagatct gccggcgctg gggatggtcc cagttccgga aggtcgggcg cgcgaccgtc
    15121 ggccgggtgg cccacgagga gccgcgcagc acgcgatagc cgccgtcgaa gaagacctgg
    15181 gagtactccg ggtaggggaa ggcgcgaaac ccgggatagc cgcgcagctc gctggccgtc
    15241 cactcccagc agtcgccgac gaaccggccc gcgtggccgg gcgcgaagtc gagctggtcg
    15301 aggttgccgc ggcgcccctc gtcgagcgca actgcggcgt gctcccactc cgcctcggtc
    15361 ggcagccgcc cgcccgccca gcgcgcgtag gcctcggcct cgaaccacga gacgtgcatc
    15421 accggcaggt ggggcgccag aggctcgagg cggtcgaacc gccgctcgtc gccgtcgccc
    15481 gtccagtaca gcggtcgctc ggcgcgctcg ccctcgcgcc acgcccagcc cgcctcgctc
    15541 cacagctccg gccgcgcata gccgccgtcg gcgacgaact gggcgtactc gccgttgctg
    15601 accggcgcgc ggtcgatcgc gtaggcgccc agctcgaccc ggtgacgagg ccgctcgttg
    15661 tcgtaggcga tctcgtcgcc cttgtcgccc atctcgatga cgcaggcgct gaaggctgcc
    15721 ggcccgtccg cgggaccttg g
//
LOCUS       DATABV010000186         4982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2310163, whole genome shotgun sequence.
ACCESSION   DATABV010000186 DATABV010000000
VERSION     DATABV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310163"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VHF88_08500"
     CDS             complement(<1..600)
                     /locus_tag="VHF88_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX2161845.1"
                     /translation="MAKDTEKLIRQLSLISFLMAERRPVSALEIRQDVEGYAQMTSDE
                     AFNRRFYADRAELASLGIELEVEKSSEGSYEAETYTLPPENFYLPAIEFSDTELGGLR
                     TALTLLDGEFAYAEPLRLALQQLSWGKPSPLSDAGQQTVRMAMTADSGGRELSQRLSK
                     IETAIFRRKTIVFDYYTLGRDSEERRKVDPYHLLFQGGQF"
     gene            733..2676
                     /locus_tag="VHF88_08505"
     CDS             733..2676
                     /locus_tag="VHF88_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX2161846.1"
                     /translation="MRILSTCVSALAVLIAGLAGAASAQAGFAPALGSPFPYAGPTVA
                     LAVADGDRNGTVDVVAGGSLSLRRGAGTGFLGNPIAVGATGPVEGLSAGDLNGDGLLD
                     YAGIAPGSTPSAPRQLLRLIATPGNGFAQEVVLADAGDATDVTIADVDGDSLLDIVIV
                     RGDDALPDPEPGDAPGPEPGPEPDEALGRQDVTVVRGGTLDERHHESGIAAPGEVEVA
                     DLNGDGRPEILVAGDEPSVSVLVNTGGGGFAAGDLSPTGAVGATRRIALAPVNGDGAL
                     DLLATDSGAAAVLALIGDGTGRFQPLGPRATGLPGPSAAVAAGDVNGDGPVDAVVGGE
                     AGFAVLLGDGGGGLSPAPGSPFPTWVAAGDPVDDVVVADMNRDAQLDVVTANRNGSVS
                     VELNADTGLLTAAPTGVDFGTMLPATGTFTQNVTLRAARGRVRLTRLDRQGSRVFDVR
                     DVDCLNRTLLLGQACTVSVTFNAPRRARRYEALLSVDANAAAVVVPLTATTRPPIVSS
                     PRLKRKRVKLGQRLLLRYGLSEGALTRVIVERARPGRRFGNRCVEPRRGNPKLRRCTL
                     WHAVGRTARRDDAGRNRIKIATRGKSRGFGRKRRLGTPYPPGTYRLSISALDRFRNRA
                     EEQTVRFKILRPKPKVKKKPARE"
     gene            2687..3379
                     /locus_tag="VHF88_08510"
     CDS             2687..3379
                     /locus_tag="VHF88_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2161847.1"
                     /translation="MTGAALVAGGAGALGSAVVAELRARGWDVTVLDRHEHGDEAAVG
                     GDGEALGRLAYVQADLLDQESAAAAVESVDGLKAVVNLVGGFSMGPKVGETDLDDFEF
                     MLRLNLFPAFNLARAAMPRLVAAGGGAFVCVSARAALEPFSRAAGYITAKAAILAFIR
                     SLDVEYRCDGVRANAVLPSVIDTPANRESMSSSDWSKWVPPEQIARVIAALCSDDLTP
                     TSGAAVPVYGRA"
     gene            complement(3393..3917)
                     /locus_tag="VHF88_08515"
     CDS             complement(3393..3917)
                     /locus_tag="VHF88_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161848.1"
                     /translation="MAKRLITLMAALLVLGFVAAGCGDDESSNDTGDTAAQTVTDTDT
                     VTEDEVVTDDEVVTDTDTVTQTDTETATQTETVTDQTDTDATGDDSGGAAVPQNREQA
                     VASCKRAVTSYPKISEDTKSDLVALCEQGASGDAEQARRARSEVCEKIVDDVYPSGVP
                     GKAKALEACQSSVK"
     gene            4017..4511
                     /locus_tag="VHF88_08520"
     CDS             4017..4511
                     /locus_tag="VHF88_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX2161849.1"
                     /translation="MGILSDDKQAERKQLIEMLTKAYWMEMETVMSYTANSINPDGVR
                     AQEIIESLEKDIQEELGHAQKFGHRIKELYGTVPGSLGFSAEQSYLQPPEDNVDIVHV
                     IKGVIEAESGAIEHYNRVIEYAEEHGDPVTADMVTTILADEEGHRRLFEGFLREYERG
                     GSTT"
BASE COUNT          695 a         1677 c         1851 g          759 t
ORIGIN      
        1 gaactggccg ccctggaaga gcaggtggta ggggtcgacc ttgcgccgct cctcggagtc
       61 gcggccgagc gtgtagtagt cgaagacgat cgtcttgcgg cggaagatcg cggtctcgat
      121 cttcgacagc cgctgcgaca gctcgcggcc gcctgagtcg gcggtcatcg ccatccggac
      181 ggtctgctgg ccggcgtccg agagcggcga cggcttgccc caggagagct gctgcagtgc
      241 cagccgcagc ggctcggcgt aggcgaactc gccgtcgagc agcgtcagcg cggtgcgcag
      301 gccgccgagc tcggtgtcgg agaactcgat cgccggcagg tagaagttct ctggtgggag
      361 ggtgtaggtc tcggcctcgt aggaaccctc gctcgacttc tcgacctcga gctcgatgcc
      421 gagcgacgcc agctcggcgc ggtcggcgta gaagcggcgg ttgaacgcct cgtcgctggt
      481 catctgggcg tagccctcga cgtcctggcg gatctcgagc gccgacaccg gccgccgctc
      541 cgccatcagg aacgagatca gcgacagctg ccggatgagc ttctctgtgt cctttgccat
      601 gaacggccca ggatacgccg gccagagcgg cgcacgggcg tgcagagcgg tagaactggc
      661 aggtcgaggc cgctatttgc ggggtcccgc aactgcggcc cttctcgcgt gtcgatgggt
      721 acggtgtctc cggtgcggat tctcagtacg tgcgtgtccg ccctggccgt cctgatcgcg
      781 gggctggccg gggccgcttc ggcacaggcg gggttcgccc cagcgctcgg ctcgccgttc
      841 ccctacgcgg ggccgacggt ggcgttggcg gtagccgacg gcgaccgcaa cggcaccgtc
      901 gacgtcgtcg ccggcggcag cctgtcgctg cggcgcggag cgggcaccgg cttcctcggc
      961 aacccgatcg cggtcggggc caccgggccg gtcgaggggc tctccgccgg cgatctcaac
     1021 ggcgacggcc tgctcgacta cgccgggatc gcgccgggaa gcacgccgag cgcaccgcgc
     1081 cagctgctgc gcctgatcgc cacgccgggc aacggcttcg cccaggaggt cgtgctcgcc
     1141 gacgccggcg atgcgaccga cgtcacgatc gccgacgtcg acggcgacag cctgctcgac
     1201 atcgtcatcg tccgcggcga cgacgcgttg cccgaccccg agccgggcga cgcgcctggc
     1261 cccgagcccg gccccgagcc cgacgaggcg ctcggacgcc aagacgtgac ggtggtccgc
     1321 ggcgggacgc tcgacgagcg ccatcacgag tccggcatcg ccgccccggg cgaggtcgag
     1381 gtcgccgacc tcaacggcga tgggcggccg gagatcctcg tcgcaggtga cgagccgagc
     1441 gtctcggtgc tcgtcaacac gggaggcggc ggctttgccg cgggcgacct ctcgccgacc
     1501 ggcgccgtcg gcgcgacccg gcgcatcgcc ctcgctccgg tcaacggcga cggcgcgctc
     1561 gacctgctcg cgaccgacag cggcgccgcc gcggtgctcg ccctgatcgg cgacgggacc
     1621 ggccgcttcc agccgctcgg tccgcgcgcg acggggttgc ccggcccctc cgcagcggtc
     1681 gccgccggcg acgtcaacgg cgacgggccc gtcgacgcgg tcgtgggcgg cgaggccgga
     1741 ttcgcggtgc tgctcgggga tggcggcggc gggctcagcc ccgcgcccgg atcgccgttc
     1801 ccgacctggg tcgcggccgg cgacccggtc gacgacgtcg tcgtggccga catgaaccgc
     1861 gacgcccagc tcgacgtcgt gaccgcgaac cgcaacggct cggtgtcggt cgagctgaac
     1921 gccgacaccg gtctgctgac ggccgcgccg accggcgtcg acttcggcac gatgctgccc
     1981 gcgaccggca cgttcaccca gaacgtcacg ctgcgcgccg cacgcggccg cgtacgcctg
     2041 acgcggctcg accgacaggg ctcgcgcgtc ttcgacgttc gcgacgtcga ctgcctcaac
     2101 cggacgctcc tgctcgggca ggcctgcacc gtctcggtca ccttcaacgc gccgcggcga
     2161 gcgcgccgct acgaggcgct gctgtcggtc gacgcaaacg ccgccgcggt cgtcgtgccg
     2221 ctcaccgcga ccacccgccc gccgatcgtg tccagtccac ggctcaagcg caagcgggtg
     2281 aagctcggcc agcggctgct gctgcgctac gggctctccg agggcgcgct cacgcgcgtg
     2341 atcgtcgagc gtgcccggcc ggggcggcgc ttcggcaatc gctgcgtcga gccacggcgc
     2401 ggcaacccga agctgcgccg ctgcaccctc tggcacgccg tcgggcgaac cgcccggcgc
     2461 gacgacgccg gccgcaaccg gatcaagatc gcgacgcgcg gcaagtcgcg tggcttcggt
     2521 cgcaagcgcc ggctgggcac gccgtatccg ccggggacct atcggctcag catcagcgcg
     2581 ctcgaccgct tccgcaaccg cgcggaggag cagaccgtgc gcttcaagat cctgcggccg
     2641 aagccgaagg tcaagaagaa gccagcgagg gaatagcttt gtcgggatga ccggcgcagc
     2701 actagtcgcg ggcggggcgg gtgcgctcgg gagcgccgtc gtcgcggagc tgcgcgcacg
     2761 cggctgggac gtgacggtgc tcgaccgtca cgagcacgga gacgaagctg cggtgggcgg
     2821 cgacggcgag gcgctcggcc ggctcgccta cgtccaagcc gacctgctcg accaggagtc
     2881 ggcggccgcc gcggtcgagt cggtcgacgg gctgaaggcg gtggtcaacc tcgtcggcgg
     2941 cttctcgatg gggcccaagg tcggcgagac cgacctcgac gacttcgagt tcatgctgcg
     3001 gctcaacctc tttccggctt tcaacctcgc gcgcgcagcg atgccgcgcc tggtcgccgc
     3061 cggcggcggc gcgttcgtct gtgtgtccgc gcgcgccgcg ctcgagccgt tctcgcgcgc
     3121 cgccggctac atcaccgcga aggcggccat cctcgcgttc atcaggtcgc tagacgtcga
     3181 gtaccgctgc gacggcgtgc gcgccaacgc ggtgctgccg agcgtgatcg acacgcccgc
     3241 gaaccgcgag tcgatgtcca gctcagactg gtcgaagtgg gtgccgcccg agcagatcgc
     3301 ccgcgtgatc gcggcgcttt gctcggacga cctgaccccc acctcgggcg cggccgtacc
     3361 tgtctacgga cgcgcctgag ccgtcgcgcg aatcacttga cgctgctctg gcacgcctcg
     3421 agcgccttcg ccttgccggg cacgccgctc ggatagacgt cgtcgacgat cttctcgcag
     3481 acctcgctgc gcgcccgacg cgcctgctcg gcgtctccag acgcgccctg ctcacaaagg
     3541 gccacgaggt ccgacttggt gtcctcggag atcttgggat aggaagtcac cgcgcgcttg
     3601 cagctcgcga ccgcctgctc ccggttctgc ggcaccgccg cgccgccgct gtcgtcccct
     3661 gtcgcatcgg tgtccgtctg gtcggtcacc gtctcggtct gggtcgcggt ctccgtgtcc
     3721 gtctgggtga cggtgtccgt gtccgtgacg acctcgtcgt cggtcacgac ctcatcctcg
     3781 gtgaccgtgt ccgtatcggt gaccgtctga gccgccgtgt cccccgtgtc gttcgagctc
     3841 tcgtcgtcgc cacacccggc agcgacgaag ccgagcacca gcagcgccgc catcaaagtg
     3901 atcagtcgct tagccatccg ttccttccgt agaggtcggg ccggaagcta gcgcgctcgt
     3961 cggcgactcg cgacgcgtca cgtttggccg gtgggaaggc gagaaagaaa gaaggcatgg
     4021 gaatcctcag cgacgacaag caggccgagc gcaagcagct gatcgagatg ctgaccaagg
     4081 cgtactggat ggagatggag acggtgatga gctacaccgc caactcgatc aaccccgacg
     4141 gggtgcgcgc gcaggagatc atcgagagcc tcgagaaaga catccaggag gagctcgggc
     4201 acgcgcagaa gttcgggcac cggatcaagg agctctacgg caccgtgccg gggtcgctcg
     4261 ggttcagcgc cgagcagtca tatctgcagc cgccggagga caacgtggac atcgtccacg
     4321 tgatcaaggg ggtgatcgag gccgagtccg gggcgatcga gcactacaac cgggtgatcg
     4381 agtacgcgga ggagcatggg gacccggtga cggcggacat ggtgacgacg atcttggcgg
     4441 atgaggaggg gcatcggagg ttgtttgagg ggtttttgag ggagtacgag cggggtgggt
     4501 ctacgacgta gggtccgggc gtcgatggct gcgggtccgg gggctggtgg ccgcgggtct
     4561 tggggctggt ggccgccggg tcctgtcagg gggaaatctc gcgtgctcaa gagggctgcg
     4621 cgcgcgagga tttccccctg ccacccgcgg gagtggcgtc gggggacggc cgggtggatt
     4681 gacgtcgtag gctggcgtgg tggctgttga ggcgcgggac gcggttgttg ttgcgacggc
     4741 taggacgccg atggggcggt atggggggca gctgagctcg gtgcggccgg atgatttggc
     4801 ggctgtggct gtgcgggagg ttgtgcggcg ggctggggtg gatgcgggtg atgtggacga
     4861 tgtgattctg ggttgtgcga atcaggcggg ggaggacaac cggaatgtgg cgcggatggc
     4921 ggggttgttg gcggggtttc cggtggaggt gccggggcag acggtgaacc ggttgtgtgg
     4981 gt
//
LOCUS       DATABV010000187         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_461479, whole genome shotgun sequence.
ACCESSION   DATABV010000187 DATABV010000000
VERSION     DATABV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461479"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1365)
                     /gene="der"
                     /locus_tag="VHF88_08525"
     CDS             complement(55..1365)
                     /gene="der"
                     /locus_tag="VHF88_08525"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX2161850.1"
                     /translation="MSLPKIAIVGYPNVGKSTLVNRLSETREAVVHPEAGVTRDRKEV
                     EADWNGRRFLLIDTGGVDMDETDDLARAVQRQARSALAEADVAVLVVDAAAGMRPGDT
                     ELARELRASQTPIVVAANKVDDALRQANAAEFYGLGLGDPVAVSAAHGLGTGDLLDRI
                     AKTLEAVPEREPETATRLAVIGRPNVGKSSLVNKLLGEERVIVADLAGTTRDSIDTRI
                     EIEARPVILVDTAGLRRRTKVAGTVDYYAQLRSEQAAERADVAIVLCDAAEGITSEDF
                     RIAELAMKANCATVVALNKWDLTETDLEDARARVGKKLRQRPKLLAVSARTGRGLTRL
                     VAEALDLADRSAQRIPTPQLNGFLADLQDVRQPPAKHGKRLRLYYLAQFETSPPRFAL
                     QVNDRSLVTRDYAYFLENRLRDRFGLDGVPLVIDIKTTAGRRNK"
     gene            complement(1410..1697)
                     /locus_tag="VHF88_08530"
     CDS             complement(1410..1697)
                     /locus_tag="VHF88_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161851.1"
                     /translation="MNVTIAGIDFAHHHYDERGDVLYLNTDGYDGTKPPFKALPTSEG
                     HSVEYDEQGRVVSLLLTNVRWLLERDGEVTISWPAGHLSSEDLSAVLQPTA"
     gene            complement(2079..>2632)
                     /gene="cmk"
                     /locus_tag="VHF88_08535"
     CDS             complement(2079..>2632)
                     /gene="cmk"
                     /locus_tag="VHF88_08535"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX2161852.1"
                     /translation="PALGFTYLDSGAMYRAVGLVTLEEGGAASDQARRLQISLGDRVI
                     ANGRDVTEAIREPRVSEAASKVATNQAVRAALVEKQRELLAEGDWVAEGRDIGTVVVP
                     DAAVKVFLVADPEERARRRAVELGANVEEVLRDQALRDAQDSSRQHSPLKPAEGSIEL
                     DTTGLTVEQVVARVADLVAAARQ"
BASE COUNT          378 a          912 c          923 g          419 t
ORIGIN      
        1 cggccccccg cacccccgcc tgtctttggc cggctggcga tgctcgccag ccagctactt
       61 gtttcggcgg ccggctgtgg tcttgatgtc gatcacgagc ggtacgccgt cgagcccgaa
      121 gcggtcgcgc aggcggttct cgaggaagta ggcgtagtcg cgggtgacca gcgagcgatc
      181 gttgacctgc agggcgaagc gcggcggcga ggtctcgaac tgggccaggt agtacaaccg
      241 cagccgcttg ccgtgtttgg cgggcggctg gcgcacgtcc tggaggtcgg cgaggaagcc
      301 gttgagctgc ggcgtcggga tccgctgcgc gctgcggtcg gcgaggtcga gcgcctcggc
      361 taccaggcgc gtcaggccgc gacccgtgcg cgccgagacg gccagcagct tcggccgctg
      421 gcgcagcttc ttgcccacgc gggcgcgggc gtcctcgagg tcggtctcgg tcaggtccca
      481 cttgttcagc gcgaccacgg tggcgcagtt ggccttcatc gccagctcgg cgatccggaa
      541 gtcctcgctg gtgatcccct cggcggcgtc gcagaggacg atcgcgacgt ctgcgcgctc
      601 agcggcctgc tccgagcgca gctgggcgta gtagtcgacg gtgccggcga ccttggtgcg
      661 gcgccgcagg ccggccgtgt cgacaaggat cacgggccgg gcctcgatct cgatccgcgt
      721 gtcgatcgag tcgcgcgtgg tgccggcgag atcggcgacg atcacgcgct cctcgccgag
      781 cagcttgttg accagcgacg acttgccgac gttcgggcgg ccgatcacgg ctagccgcgt
      841 cgccgtctcc ggctcgcgct cgggcaccgc ctcgagcgtc ttggcgatcc ggtcgagcag
      901 gtcgccggtg ccaagcccgt gggcggccga gacggcgacc gggtcgccga gcccgaggcc
      961 gtagaactcg gcggcgttgg cctggcgcaa cgcgtcgtcg accttgttcg ccgcgacgac
     1021 gatcggcgtc tgggaggccc gcagctcgcg cgccagctcc gtgtcccccg gccgcatccc
     1081 ggcggccgcg tcgacgacca gcaccgcgac gtcggcctcg gcgagcgcgc tgcgcgcctg
     1141 gcgctggacg gcacgagcga ggtcgtctgt ctcatccatg tcgacccccc cggtgtcgat
     1201 cagcaggaag cgccgcccgt tccagtccgc ctcgacctcc ttgcggtcgc gcgtaacccc
     1261 cgcctccgga tgcacgaccg cctcgcgcgt ctcggacagc cggttcacca gcgtcgactt
     1321 gccgacgttc gggtagccga cgatcgcgat cttcgggagg gacatgggcc ggtgaggcta
     1381 ctggcgggat gcggtcgact cggaagcgcc taagccgtgg gctgcagaac agctgacaag
     1441 tcctcggagc tcaggtggcc ggcgggccag ctgatcgtga cctcgccatc gcgctccagc
     1501 agccaacgca cgttggtcaa gagcaggctg acgacgcgtc cttgctcgtc gtactcgacg
     1561 ctgtgcccct ccgacgtggg caaagccttg aacggaggct tggtgccgtc gtagccgtcg
     1621 gtgttcaagt agaggacgtc accacgctcg tcgtagtggt gatgggcgaa atctatgcct
     1681 gcgatggtca cgttcatgag aaagctcgcc ggggatccac gtcctgcgcg aacgccgtca
     1741 cgacccagcc gggcacatct ccgaagtcta cgaccactcg cagccagcgc atcgggtcca
     1801 ggacgtgacg ccggtagaac cgttctcgcc cgggtcgcgg gtccaactca cggaagtctg
     1861 ggctctcgac ggtggcgagc acgagttcta cgctgtccaa caatgcggga cggctgaggg
     1921 ccagatgaag gtgcgatcca gagtcgaata cgacttcccg tccgtcggga tcgaccactg
     1981 accgtcgttc cgctcccatc catccgaacg tatggattgt ctagcgacga ctcgatgccc
     2041 cacggcgacg atccagccac gaacgagcgc cagccttact actggcgggc ggctgcgacg
     2101 aggtcggcga cgcgtgcgac gacctgctcg acggtcaagc cggtcgtgtc gagctcgatc
     2161 gacccttcgg ccggcttgag cggcgagtgc tggcgactgg agtcctgggc atcgcgcagc
     2221 gcctgatcgc gcaggacctc ctcgacgttg gcgccgagct cgacggcccg gcgccgggcg
     2281 cgctcctcgg gatcggcgac gaggaagacc ttgacggcgg catcgggaac gacgaccgtg
     2341 ccgatgtcgc ggccctcggc gacccagtcg ccctcggcga gcagctcgcg ctgcttctcg
     2401 acgagcgccg cccgcaccgc ctggttcgtg gcgaccttcg acgctgcctc ggagacacgc
     2461 ggctcgcgga tggcctctgt cacgtccctg ccgttggcga tcacccgatc gccaagcgat
     2521 atctggagtc gccgcgcctg gtcggacgcg gcgccaccct cctccagggt gaccagcccg
     2581 acggcgcggt acatggcccc cgagtcaagg taggtgaacc cgagcgcggg gg
//
LOCUS       DATABV010000188         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_461672, whole genome shotgun sequence.
ACCESSION   DATABV010000188 DATABV010000000
VERSION     DATABV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461672"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VHF88_08540"
     CDS             complement(<1..403)
                     /locus_tag="VHF88_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2161853.1"
                     /translation="MSQQSFADLGVSSPVLSALEKRGFKAPFAVQRLVIEDVLAGHDV
                     LAQSPTGSGKTIAFGVPLIDRIAADSRRPAGLILAPTRELACQIVDELYEIAHSRALS
                     VAPVYGGVGIQAQARKAAKAHIVVATPGRLED"
     gene            complement(550..825)
                     /locus_tag="VHF88_08545"
     CDS             complement(550..825)
                     /locus_tag="VHF88_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161854.1"
                     /translation="MIGFIVAGIVIGILARLILPGRQRIGILMTVLAGVLGSIVGGTV
                     ANALGTGSIWELNFIGFICATGAAALFVAAAEGLQLGGGRERRQLGR"
     gene            complement(866..2137)
                     /gene="rmuC"
                     /locus_tag="VHF88_08550"
     CDS             complement(866..2137)
                     /gene="rmuC"
                     /locus_tag="VHF88_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX2161855.1"
                     /translation="MGQLWLLIGLALGAGVVYLVMRGRVEHERRVAAEKVEAVTAARD
                     ELANQFTALSAAALEQNNARFMQLAEERLKAQQAAGRTELDSQKRGVEQLVKPVEEKL
                     EAVNRVLREMEKERAQAYGGLNRQLVEVTETQQRLHAETANLVTALRAPHVRGRWGEV
                     QLKRVCEMAGMLAHCDFYEQQSVSGDNGVLRPDVVVKLPGGKDVVVDAKAPLAAYLDA
                     IEAKDEATREAHLDVYARHVREHIQKLSAKSYAAQFDSSPEFVVMFLPGEVFYSAALE
                     RMPDLIERSVEESVLIATPTTLIALLKAVAYGWRQERMAESAREVSALGRELYQRLSV
                     LSDRFVTLGRRLDSSVKAYNDTVGTLESRVLVSARKFDDHGVAPDGAELDSPAPVELS
                     VRRLRAPGVDGEPELEANGPRELPRTAEELL"
     gene            2261..3250
                     /locus_tag="VHF88_08555"
     CDS             2261..3250
                     /locus_tag="VHF88_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2161856.1"
                     /translation="MTDRVRLGRSELMVSPIAFGTWQLGGEWGSFDEEAAIAAIRRAR
                     ELGINFFDTAQAYGFGVSERLLGEALADELRDARDDVVIATKGGLRQVDDGLERDSSP
                     GFLRDGVERSLEALGVDHIDLYQVHWPDPGTPFAETGAVLAELRDEGKIRHIGVSNYS
                     AEQMAELEQAVPVETLQPPYHLFNRDIEESVLPWCLDHDVGVLVYGPLAHGLLTGTIG
                     EDAEFADDDWRSTSPLFAHVNLQQNVKVVRQLAEVADDLGCTLSQLAIAWTLAHPAVH
                     VAIVGSRNADHIAESIGAVDVELGDEELQRIVTIMQGAVGAEGPSPEGPTSAE"
     gene            complement(3280..>3574)
                     /locus_tag="VHF88_08560"
     CDS             complement(3280..>3574)
                     /locus_tag="VHF88_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2161857.1"
                     /translation="VATLAELETGVLMAKDDDSRAIRMQTLVSVRERAAGIPADQQVA
                     SAYARLASAALRSGKKPRVQDTWIAATALVHNAEVWTQDEDFTEFPSVDVVRV"
BASE COUNT          549 a         1269 c         1235 g          521 t
ORIGIN      
        1 gatcctcgag ccggcccggc gtggcgacga cgatgtgcgc cttggcggcc ttgcgcgcct
       61 gggcctggat gccgacgccg ccgtagaccg gcgccaccga gagcgcacgc gaatgcgcga
      121 tctcgtagag ctcgtcgacg atctggcagg cgagctcgcg cgtcggcgcg aggatcaggc
      181 cggcggggcg gcgcgagtcg gccgcgatgc ggtcgatcag cggaacgccg aaggcgatcg
      241 tcttgccgga cccggtcggc gactgggcga ggacatcatg gccggcgagc acgtcctcga
      301 tgacgaggcg ctggaccgcg aagggcgcct tgaagccgcg cttctccagc gcggacagca
      361 cgggacttga tacgccgaga tcggcgaagg actgctgaga cattgactgc gactccttga
      421 gtgcgacggg gagatcccac taccccggac gcgttgagac gcagcaaagg acgggctctc
      481 tcgggcgcca cggtggcggc cgacccgctg accgtagcag gaatgctgga aaagcctgct
      541 cgctgggcgc tagcgcccga gctgtcggcg ctcgcgccct ccgccgagct gcagcccctc
      601 ggcggcagcg acgaacaacg cggcggcgcc cgtggcacag atgaagccga tgaagttcag
      661 ctcccagatg ctgccggtcc cgagcgcgtt ggcaaccgta cccccgacga tcgagccgag
      721 cactccggcg aggaccgtca tcaggatgcc gatccgctgg cggccgggaa ggatcaagcg
      781 agcgaggatg ccgatcacga tgccggcgac gatgaatccg atcatgggct caacctaccc
      841 ctagtccggc agttcccaac cccgatcaga gcagttcctc ggccgtgcgc ggcagctcgc
      901 gcggcccgtt ggcctcgagc tctggctcgc cgtcgacgcc cggcgcgcgc agccgtcgca
      961 ccgacagctc gaccggcgcg ggcgagtcga gctcggcacc gtcgggcgcc acgccgtggt
     1021 cgtcgaactt gcgtgccgag accagcacac ggctctcgag cgtgccgacg gtgtcgttgt
     1081 aggccttgac cgacgagtcg agccggcgcc ccagcgtgac gaagcggtcg ctcagcaccg
     1141 acaggcgctg gtagagctcg cggccgagcg ccgagacctc gcgcgccgac tcggccatcc
     1201 gctcctggcg ccagccgtag gcgacggcct tcaacagcgc gatcagcgtc gtcggcgtcg
     1261 cgatcagcac cgactcctcg accgagcgct cgatcaggtc gggcatccgc tcgagcgcgg
     1321 ccgagtagaa gacctcgccc ggcaggaaca tcacgacgaa ctccggcgaa gagtcgaact
     1381 gcgccgcgta cgacttggcc gacagcttct ggatgtgctc gcggacgtgg cgggcgtaga
     1441 cgtccaggtg cgcctcacgg gtcgcctcgt ccttcgcctc gatcgcgtcg aggtaggcgg
     1501 cgagcggcgc cttggcgtcg acgacgacgt ccttgccgcc gggcagcttg acgaccacgt
     1561 ccggacgcag caccccgttg tcgccgctga ccgactgctg ctcgtagaag tcgcagtggg
     1621 cgagcatgcc cgccatctcg cagactcgct tgagctgcac ctcgccccag cggccgcgga
     1681 cgtgcggcgc gcgcaacgcc gtgacgaggt tggcggtctc ggcgtgcagg cgctgctggg
     1741 tctcggtgac ctcgaccaac tggcggttca ggccgccgta ggcctgggcg cgctccttct
     1801 ccatctcgcg cagcacccgg ttgaccgcct cgagcttctc ctcgaccggc ttgacgagct
     1861 gctcgacgcc gcgcttctgg ctgtcgagct ccgtcctgcc ggcggcctgc tgggccttca
     1921 gccgctcctc ggcgagctgc atgaagcgcg cgttgttctg ctcgagggct gccgccgaca
     1981 gcgccgtgaa ctggttcgcg agctcgtcgc gcgccgcggt gacggcctcg accttctcgg
     2041 ccgctacccg ccgctcgtgc tcgacccgcc cgcgcatcac gagatacacc acgcctgcgc
     2101 cgagcgccaa gccgatcaac aaccacagct gccccatcag ttccccttcg tctccattcg
     2161 agtcgtccag cgttgcgcag acccaggacg gaaacccatg gatgccggct ccttccgttt
     2221 ggctcgccgg ggccgagggc acttccaaga ggtggctgga gtgacggatc gggtgcgcct
     2281 cgggcgcagc gaactgatgg tctcgccgat cgccttcggg acctggcagc tcggcggaga
     2341 gtggggcagc ttcgacgagg aggcggcgat cgccgcgatc cgccgcgcgc gcgagctggg
     2401 gatcaacttc ttcgacaccg cccaggcgta cgggttcgga gtgtcagagc ggctgctcgg
     2461 cgaggcgctc gccgacgagc tgcgcgatgc gcgcgacgac gtggtgatcg cgaccaaggg
     2521 cggcctgcgc caggtcgacg acggcctcga gcgcgactcg agtcccgggt tcctgcgcga
     2581 cggcgtcgag cgcagcctgg aggcgctcgg cgtcgaccac atcgacctct accaggtcca
     2641 ctggcccgac ccgggcaccc cgttcgccga gaccggtgcc gtgctggccg agctgcgcga
     2701 cgagggcaag atccgccaca tcggcgtctc gaactactcc gcggagcaga tggccgagct
     2761 ggagcaggcc gtcccggtcg agacgctgca gccgccgtac cacctgttca accgcgacat
     2821 cgaagagagc gtgctgccgt ggtgcctcga ccacgacgtc ggagtgctcg tctacggccc
     2881 gctcgcgcac gggctgctga ccgggacgat cggcgaggac gccgagttcg ccgacgacga
     2941 ctggcgctcg accagcccgt tgttcgccca cgtcaacctg cagcagaacg tcaaagtcgt
     3001 gcgccagctc gccgaggtcg ccgacgacct cggctgcacg ctgagccagc tcgcgatcgc
     3061 ctggacgctc gcccatccgg cggtccacgt cgcgatcgtc ggctcgcgca acgccgacca
     3121 catcgccgag tcgatcggcg cagtcgacgt cgagctcggc gacgaggagc tgcaacggat
     3181 cgtgacgatc atgcagggcg ccgtcggcgc cgagggcccc tcacccgagg gccccacctc
     3241 ggccgagtga ccagcgtcag ggccagacca gcgttcgggt cagacgcgta cgacgtcgac
     3301 ggacgggaat tccgtgaagt cttcgtcctg ggtccacacc tcagcgttgt ggacgagggc
     3361 ggtcgccgcg atccaggtgt cctggacccg tggcttcttg ccactccgta gggcagcgga
     3421 ggcgagccgg gcataggcgg aggcgacctg ctgatctgcg gggattccgg cagcccgctc
     3481 cctgaccgag acgagagtct gcatgcggat cgcccgcgag tcgtcgtcct tggccatcag
     3541 cacgcccgtc tcgagctcgg ccagagtcgc gacc
//
LOCUS       DATABV010000189        11458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2791270, whole genome shotgun sequence.
ACCESSION   DATABV010000189 DATABV010000000
VERSION     DATABV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791270"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1340
                     /gene="metK"
                     /locus_tag="VHF88_08565"
     CDS             90..1340
                     /gene="metK"
                     /locus_tag="VHF88_08565"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX2161858.1"
                     /translation="MSISDFADNEFLFTSESVTEGHPDKIADRISDSVLDAVLAADPA
                     GRVACETLVNTGLIVISGEISTTADLDFQAIARNAVDEIGYNDAAYGFDCNTCAVMVT
                     VDKQSPDIAQGVDQAYEKRADGSGDDFDIAGAGDQGMMFGYASNETPELMPAPIALAH
                     RLSERLSEVRHDGTLGYLRPDGKTQVSVRYRDGRPVAVEKVLISTQHAEDVEQPQIKA
                     DLWDNVVVPVLDREGVPYDAATLREGHNFLVNPTGKFVIGGPMGDCGLTGRKIIVDTY
                     GGAARHGGGAFSGKDPSKVDRSAAYAARWVAKNVVAAGLADRCEVQVAYAIGVAHPVS
                     VMVETFGTEKIGRARIAELVDEHFDLRPAAFRRELDLHRPIFRKTSAYGHFGRELDEF
                     TWEKTDKADALREAAGLAEAAPVA"
     gene            complement(1337..1576)
                     /locus_tag="VHF88_08570"
     CDS             complement(1337..1576)
                     /locus_tag="VHF88_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161859.1"
                     /translation="MSRLPHAAARELPEQDAVAAADLDDAARRARQRGDQLENVVGLA
                     GGAELAPGAVAAGVIARRRVLALVDSAEVDFPSAC"
     gene            1481..2185
                     /locus_tag="VHF88_08575"
     CDS             1481..2185
                     /locus_tag="VHF88_08575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2161860.1"
                     /translation="MPRPPRSVVEVGCGDGVLLGELARRGVGESRHGFEISERAVAYA
                     TSRPGVDRAERFDGHTLPVADHAYDLGILSHVLEHVPDPLPLLRETARACEAVVVEVP
                     LEDNVTASRSSASAGRAAIGHLHRFSRADVSRLASAAGLQVVAELADPLPLAVHSFFA
                     DTPAVKAKALAKTAARRAVFAAAPTRAERLFTVHYAALCLPSTTREVLGPQARQTLRR
                     PPDNGCPPAPRPRLTG"
     gene            2182..4383
                     /gene="priA"
                     /locus_tag="VHF88_08580"
     CDS             2182..4383
                     /gene="priA"
                     /locus_tag="VHF88_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX2161861.1"
                     /translation="MIAKVEPLTTARALRGPFDYRVGQGLLDGGVGVGSVLVVPFGPR
                     RMLGVVVDVAETSELAPERLAEPIEALEADVPAELVRLGLWVAQEYVSTPARGLALVL
                     PPGTGTGGGRRTKARESLSATLTDAGRAALETGARLGSRQRAGLEALVDGPLRVSDLG
                     RLSGCDHAGVRRLEQRGLVSIERSVEAPRRPTMRGVGARTTVRELTPDQSAALAQIVA
                     SLPARRSGIPPPRTAAPAPMLLHGVTGSGKTEVYLRAVEEVLARGQTAIVLVPEIGLT
                     PQTAGRFVERFGDTVAVLHSQLTARERYDEWSRLRSGTARVAVGPRSAVFAPLQDVGL
                     IVVDEEHDGSYKQEGDPRYDARTVAERRARECGALLLAGSATPRPESVLRYERIRLPE
                     RVDGRPLPPVELVGMAATHGPLHQRTRDALEEVRRANGKAIVLLNRRGWSNFLTCGDC
                     GRVWECPNCDVTLVLHKAAGSVACHHCGHRERVPDRCPDCSAVSLTRHGVGTEQLTDE
                     LERLMDPLPVVRLDADTAAGGGAAEALERFEAAEGGVLVGTQMVAKGHDFPDVTLGVV
                     LDADATLRFPDFRAEERTFALVAQLAGRSGRGGSEGRVVVQALDPDAAALRFAAGHDA
                     DGFLDVELTRREALSYPPYGSLIRVVCSSEQVAPAQAAAERVRAAVRGAEIPVLGPAP
                     LFRLKGHERFQLVVKATDRAAAIVAVRGAVEATAADRSLGPVKYAVDVDPN"
     gene            4405..5010
                     /gene="def"
                     /locus_tag="VHF88_08585"
     CDS             4405..5010
                     /gene="def"
                     /locus_tag="VHF88_08585"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX2161862.1"
                     /translation="MADERDEEVEEEPEVQLDPEVLARRAAAMSFIRQFGDPVLKSRA
                     IEVDRFDDALRTQVSRMAGLMDDALGVGLAAPQLGISQRLLVYRIGPDAPVVALVNPE
                     VEWSSDEDETGEEGCLSIAGVNVDVDRAVHVRVRARDELGEPRTVEASGLEARVIQHE
                     IDHLDGTLILDRTSRDQRRRAMRELRAREREERERAAERAA"
     gene            5019..5936
                     /locus_tag="VHF88_08590"
     CDS             5019..5936
                     /locus_tag="VHF88_08590"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX2161863.1"
                     /translation="MRTVYVGTSDFAAVVLDRLADSPHRPRLVVSRPDRRRGRGRKLS
                     PPPVVERARELAIDTFQPDSVNAPEALERIAAAQPEAACVCAYGAILGDELLAAHRWL
                     NVHPSLLPRWRGAAPIERAIEAGDDETGVAIMEPVAELDAGPVYLMRPEPIRPDDDYG
                     SLSARLAALGGDLLVEALDTGAAPRPQGDEGVSYAEKIDRDERLLDPALDASTLERRV
                     RALTPHVGAFVEPDGHERLGVRRVAIADDVGSAAPTPAPGTLAARDGRLLYGAADGPL
                     ELLEVQPAGGRPMDAADWLRGHGAALAAG"
     gene            5972..6202
                     /locus_tag="VHF88_08595"
     CDS             5972..6202
                     /locus_tag="VHF88_08595"
                     /inference="COORDINATES: protein motif:HMM:NF018848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX2161864.1"
                     /translation="MAGEERIDLGPPCPSCGEPWLRATNLPGRYRCVYCLSRFELRSQ
                     CPDCGSHATIARMSDLVNVSCRNCGGSLLTGV"
     gene            complement(6207..6665)
                     /locus_tag="VHF88_08600"
     CDS             complement(6207..6665)
                     /locus_tag="VHF88_08600"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX2161865.1"
                     /translation="MSRRPAVGEPAPDFTLVGTAGPFTLSDHRGEKVVLLFYPGDDTP
                     VCTRQFCSYRDNAETFGALAATVVGISGKGLDSKQAFADKHGLTVPLLADPDQSVAKS
                     YDCWSKLRGTRRAVVIVDEQGTVRHRHDHALGLDFQTAADIRQALEQLSA"
     gene            complement(6692..8026)
                     /locus_tag="VHF88_08605"
     CDS             complement(6692..8026)
                     /locus_tag="VHF88_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161866.1"
                     /translation="MPTAAGGDGSYGKGAAVLSVGIGVTGLITYAYFALASHSLSESD
                     YGAITLLWSAVFVTVSVLYRPVEQLLSRTIADRAARSATGRSHLRVAATIQLGLGIAF
                     AVAALALRGPLQDDLFDGSETLYWVLIVTVLAYAVSYFARGFLAGSHRFGLYGGLVFM
                     EAVSRCLFALAVAVGVLSGQSVVALGIAAAPIASLAVVPWALGRRIAGGLTDAEAADA
                     RRLDEAAAGEPGGEPEFTLSHGAGFAVAVLAIMLAEQTFLNAGPLIVKATTDGPDGAA
                     LAGFTFNVLLIARAPLQLFQAIQTSILPHLTRLRAGGESDPFRRSVNVTLLAIALFAS
                     AVALVMLAIGPWLMDLVFGGELAYDRIGLVLISLGMGLYLAAATLNQALLARARARAA
                     CVCWLAAAAAYVGFLLLPGFDDRVFQVEVGYAGAALVLCALLYALYRRTGDS"
     gene            complement(8027..8662)
                     /locus_tag="VHF88_08610"
     CDS             complement(8027..8662)
                     /locus_tag="VHF88_08610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2161867.1"
                     /translation="MTGVATPGSTGAEAVPTGNTFDKYGSTNPVVRRLMTAFHHTLDE
                     LWDRAAPTSVLDVGCGEGVLTHEWAQRLGDGRIVGIDLDDAKLRAEWDKRRRANLEYR
                     CEEATRLSFADGEFDMATAIEVLEHVPEPEATVAEMARVASRHLLVSVPREPLWRGLN
                     MARGAYWGSLGNTPGHVNHWSKRGFVELLSRHGEVVEARSPFPWTMLLVRL"
     gene            complement(8659..9714)
                     /locus_tag="VHF88_08615"
     CDS             complement(8659..9714)
                     /locus_tag="VHF88_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2161868.1"
                     /translation="MKLIIQIPCFNEAEQLPATLADLPRAVPGVDEVEWLIIDDGSTD
                     ATVEVARAHGVDHVVRLTNNKGLAAGFQAGLDACLKLGADLIVNTDADNQYHGGDIPK
                     LVEPILAGRADMVVGDRETDSIEHFSPLKKRLQRLGSAVVRRASGTTVPDTTSGFRAY
                     NREAALQMQVISKFTYTLETIIQAGKVLVAVDHVPIRTNEKTRESRLFPSMSQYVRRN
                     AVAIFRVYTLYEPLRVFLIAAGIVAVPAAVIWARFLFFFFNGEGNGHVQSLILGSTLL
                     IIAVQFVALGVIADVLAASRTLQQRTLERVRRVELTLGVEPSHYEPGAGDSAQPATTG
                     AAAGRATGKSDRETVEA"
     gene            9825..10001
                     /locus_tag="VHF88_08620"
     CDS             9825..10001
                     /locus_tag="VHF88_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161869.1"
                     /translation="MHLLAYLDPGSGSMILQILAGGVAAVAVTAKLYWSRLLKLLRIR
                     KDDEPTPEQQADSA"
     gene            10201..>11458
                     /locus_tag="VHF88_08625"
     CDS             10201..>11458
                     /locus_tag="VHF88_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161870.1"
                     /translation="MRTELLPSTADVPSELLVKDCAGVLRHERIPFVSYPYEWTFSML
                     KEAALLQLDLNLAALEEGMILKDSTPYNVQFKGSKPVFVDVGSFERLRDGEPWVGYRQ
                     FCMMYLYPLMLQAVKGIEFHPWLRGSIGGITPAQMRGMVSFRDRFRRGFFLNVFMHAK
                     LEARHADRGREVKAEVKQAGFKKELIVANVRRMRKLVESLTWNPPKGVWVAYGERNSY
                     SDEDAKRKDEFVREVVTAQPWRLTWDIGANNGRYSRIAEEGSDYVLAVDADQGPVELL
                     HRTLREEGDERILTLTMNLADPSPSLGWRGLERKDLPARGKPDLVLALALIHHVTIAA
                     NVPVAEFVDWLASLGTSLVIEFPTREDPMVKKLLGPKREGLHPDYERVNFERVLAGAF
                     DVERRERSKSGTRLLYFARPKGAGSPI"
BASE COUNT         1632 a         4239 c         4042 g         1545 t
ORIGIN      
        1 cccccccccc ccccccctgc tggtctgtac cctttctcga ccttcagagc cttgaccgag
       61 cccccggacc ccggagcgag cccctcccca tgagcatcag cgatttcgcc gacaacgagt
      121 tcctcttcac ctcggagtca gtcaccgagg gacacccgga caagatcgcc gatcggatct
      181 ccgactccgt gctcgacgcg gtgctcgccg ccgatcccgc cggtcgggtc gcctgcgaga
      241 cgctcgtcaa caccggcctg atcgtgatct cgggcgagat ctcgaccacc gcggacctcg
      301 acttccaggc gatcgcgcgc aacgctgtcg acgagatcgg ctacaacgac gccgcctacg
      361 gcttcgactg caacacctgc gccgtgatgg tgacggtcga caagcagtcc cccgacatcg
      421 cccagggcgt cgaccaggct tacgagaagc gcgccgacgg ctcgggcgac gacttcgaca
      481 tcgcgggcgc cggcgaccag ggcatgatgt tcggctacgc ctcgaacgag acccccgagc
      541 tgatgcccgc gccgatcgcg ctcgcccacc gcctctcgga gcggctctcc gaagtccgcc
      601 acgacggcac gctcggctac ctgcgccccg acggcaagac ccaggtctcc gtccgctacc
      661 gcgacggccg gccggtcgcc gtcgagaagg tgctgatctc gactcagcac gccgaggacg
      721 tcgagcagcc gcagatcaag gccgacctct gggacaacgt cgtcgtcccg gtgctcgatc
      781 gcgagggagt gccctacgac gctgcgacgc tgcgcgaggg ccacaacttc ctcgtcaacc
      841 cgaccggcaa gttcgtgatc ggcggcccga tgggcgactg cggcctgacc ggccgcaaga
      901 tcatcgtcga cacctacggc ggcgcggccc gccacggcgg cggggcgttc tcgggcaagg
      961 acccgtcgaa ggtcgaccgc tcggccgcct acgccgcccg ctgggtcgcc aagaacgtcg
     1021 tagcggccgg cctcgccgac cgctgcgagg tacaggtcgc atacgcgatc ggcgtcgcgc
     1081 atccggtctc ggtgatggtc gagacgttcg gcaccgagaa gatcggccgc gcgcggatcg
     1141 ccgagctggt cgacgagcac ttcgacctgc gtcccgccgc cttccgccgc gagctcgacc
     1201 tgcaccgccc gatcttccgc aagacctccg cctacggcca cttcggccgt gagctcgacg
     1261 agttcacctg ggagaagacg gacaaggccg acgcactgcg cgaggccgcc ggtctcgcag
     1321 aggccgcgcc ggtcgcctag caagcggagg ggaaatcgac ctccgcgctg tctacgagcg
     1381 ccagtactcg gcggcgggcg atgacgcccg ccgctacggc gcctggcgcg agctctgcgc
     1441 cgccggcaag gccgaccacg ttctcgagct gatcgccgcg ctgccgcgcc cgccgcgcag
     1501 cgtcgtcgag gtcggctgcg gcgacggcgt cctgctcggg gagctcgcgc gccgcggcgt
     1561 gggggagtcg cgacacggct tcgagatctc cgagcgggcg gtcgcctacg cgacgtcacg
     1621 cccgggcgtc gatcgcgccg agcggttcga cggccatacg cttccggtgg ccgaccacgc
     1681 ctacgacctc ggcatcctct cgcacgtcct cgagcacgtc cccgatccgc tgccgctgct
     1741 ccgcgagacg gcccgcgcat gcgaagcggt ggtcgtcgag gtgccgctcg aggacaacgt
     1801 gaccgcctcg cgctcctctg cctccgccgg tcgcgccgcg atcggtcacc tccaccgctt
     1861 ctcgcgcgcc gacgtgtccc gcctcgcgag cgccgccggc ctccaggtcg tcgccgagct
     1921 cgccgacccg cttccgctgg ccgtccactc cttcttcgcc gacaccccag ccgtgaaagc
     1981 gaaggcactc gccaagaccg ccgcccgccg cgccgtgttc gccgccgcac cgacgcgcgc
     2041 ggagcgcctg ttcacggtcc actacgccgc cctttgcctc ccttcgacga cccgcgaggt
     2101 tttagggccc caagcccggc aaacccttcg ccggccgccg gacaacggct gtccgcccgc
     2161 gccgcgccct agattgacgg ggtgatcgca aaggtcgagc cgctgacgac cgcccgtgcc
     2221 ctgcgcggcc cgttcgacta cagggtcggc cagggcctgc tcgacggcgg ggtgggggtg
     2281 ggcagcgtcc tcgtcgtgcc gttcggccca cgccggatgc tcggcgtcgt ggtcgacgtg
     2341 gccgagacca gcgagcttgc gcccgaacgg ctcgccgagc cgatcgaggc gctcgaggcc
     2401 gacgtccccg ccgagctcgt gcggctcggt ctctgggtcg cgcaggagta cgtctcgacg
     2461 cccgcgcgcg gcctcgcgct cgtgctgccg ccggggaccg gcacgggcgg cgggcgccgc
     2521 accaaggcgc gcgagtcgct ctccgcgacg ctgaccgacg ccgggcgcgc agcgctcgag
     2581 accggagccc gactcggcag ccgccagcgc gcgggcctcg aggcactcgt cgacggcccg
     2641 ctgcgcgtca gcgacctcgg ccgcctcagc ggctgcgacc acgccggcgt ccgacggctc
     2701 gagcagcgcg gcctggtgtc gatcgagcgc tcggtcgagg cgccgcgccg cccgaccatg
     2761 cgcggcgtcg gcgcccgcac cacggtccgc gagctcacgc ccgaccagag cgccgcgctc
     2821 gcccagatcg tcgccagcct cccagcgaga cgcagcggca tcccaccgcc gcgtaccgcg
     2881 gcccccgcgc cgatgctcct ccacggcgtc accggcagcg gcaagaccga ggtctacctg
     2941 cgcgccgtcg aggaggtcct cgcgcgcggc cagacggcga tcgtcctggt gccggagatc
     3001 ggtctcacgc cgcagacggc cgggcgcttc gtcgagcgct tcggcgacac cgtcgccgtc
     3061 ctgcactccc agctgaccgc gcgcgagcgc tacgacgagt ggtcgcggct gcgcagcggg
     3121 accgcgcggg tcgcggtcgg tccgcgctcg gccgtgttcg ccccgctgca ggacgtcgga
     3181 ctgatcgtcg tcgacgagga gcacgacggc tcctacaagc aggagggcga cccgcgctat
     3241 gacgcgcgca ccgtcgccga gcgccgcgcg cgcgagtgcg gcgcgctgct gctggccggc
     3301 agcgccacgc cgcggccgga gagcgtgctg cgctacgagc ggatccgcct gcccgagcgg
     3361 gtcgacggtc gtccgctccc gcccgtcgag ctggtcggca tggccgcaac ccacggcccg
     3421 ctgcaccagc gcacgcgcga cgcgctcgag gaggtgcggc gagccaacgg caaggcgatc
     3481 gtcctgctca accgccgcgg ctggtcgaac ttcctcacct gcggcgactg cggccgcgtc
     3541 tgggagtgcc cgaactgcga cgtcacgctc gtcctgcaca aggcggccgg ctccgtcgcc
     3601 tgccaccact gcgggcaccg cgagcgcgtc ccggaccgct gccccgactg cagtgctgta
     3661 tcgctgaccc gccacggggt cggcaccgag cagttgaccg acgagctcga gcggctgatg
     3721 gacccgctgc cggtcgtccg cctcgatgcc gacaccgccg caggcggcgg tgcggcagag
     3781 gcgctcgagc ggttcgaggc ggccgagggc ggagtgctgg tcggcacgca gatggtcgcc
     3841 aagggccacg acttcccaga cgtcacgctc ggcgtcgtgc tcgacgccga tgcgacgctg
     3901 cgcttcccgg acttccgcgc cgaggagcgg acgttcgcgc tcgtcgccca gctcgccggc
     3961 cgcagcggcc gcggcggcag cgagggccgg gtggtcgtcc aggcgctcga ccccgacgcc
     4021 gcggcgctgc gcttcgccgc cgggcacgac gccgacgggt tcctcgacgt cgagctgacc
     4081 cggcgcgagg cgctctccta cccgccctac ggctcgctga tccgcgtcgt ctgctcgtcg
     4141 gagcaggtcg cgccggccca ggccgcggcg gagcgcgtgc gcgccgctgt tcgcggggcg
     4201 gagatcccgg tgctcggccc tgcgccgctc ttccggctca agggacacga gcgcttccag
     4261 ctcgtcgtca aggcgaccga ccgcgccgcg gcgatcgtcg ccgtccgcgg cgccgtcgag
     4321 gccaccgcgg ccgaccgctc gctcggcccg gtcaagtacg ccgtcgacgt cgaccccaac
     4381 tgagcggccg gtacgatggg tgagatggcg gacgagcgcg acgaggaggt cgaggaggag
     4441 ccggaggtcc agctcgaccc ggaggtcctc gcccgtcgcg cggctgcgat gtcgttcatc
     4501 cgccagttcg gcgaccccgt gttgaagagc agggcgatcg aggtggaccg cttcgacgac
     4561 gcgctgcgga cccaggtgtc gcgcatggct ggtctgatgg acgacgcgct cggcgtcggt
     4621 ctggcagcgc cgcagctcgg catctcacag cggctgctgg tctaccggat cgggcccgac
     4681 gcgccggtgg tggcgctcgt caacccggag gtcgagtgga gcagcgacga ggacgagacc
     4741 ggcgaggagg gctgcctgtc gatcgccgga gtcaacgtcg acgtcgatcg tgcggtccac
     4801 gtccgggtgc gcgcccgcga cgagctcggc gagccgcgga ccgtcgaggc gtcgggcctc
     4861 gaggcgcgcg tgatccagca cgagatcgac cacctcgacg gcacgctgat cctcgaccgc
     4921 acctcgcgcg accagcgccg gcgggcgatg cgcgagctgc gcgcccgcga gcgcgaggag
     4981 cgcgagcgcg cggccgagcg cgccgcctga gcgccggcgt gcgcaccgtc tacgtcggca
     5041 cctccgactt cgccgcagtc gtgctcgacc gcctcgccga ctcgccgcac cggccacggc
     5101 tggtcgtgag ccgtcccgac cgccggcgcg gacgcgggcg caagctctca ccgccgccgg
     5161 tcgtcgaacg cgcacgcgag ctcgccatcg acaccttcca gcccgacagc gtcaacgcgc
     5221 ccgaggcgct cgagcggatc gccgccgccc agcccgaggc cgcctgcgtc tgcgcctacg
     5281 gggcgatcct cggcgacgag ctgctcgccg cgcaccgctg gctcaacgtc catccgtcgc
     5341 tgctgccgcg ctggcgcggc gcggcgccga tcgagcgggc gatcgaggcg ggcgacgacg
     5401 agaccggcgt cgcgatcatg gagccggtcg ccgagctcga cgccggcccc gtctatctga
     5461 tgcgtccgga gccgatccgt cccgacgacg actacggcag cctctcggcc cgcctcgccg
     5521 cacttggcgg cgacctgctc gtcgaggcgc tcgacacagg cgccgctccg cgcccgcagg
     5581 gtgatgaggg cgtcagctac gccgagaaga tcgaccgcga cgagcggctg ctcgaccccg
     5641 cgctcgacgc gtcgacgctc gagcggcggg tgcgcgcgct caccccgcac gtcggcgcct
     5701 tcgtcgagcc ggacggtcac gagcgactcg gcgtgcgacg ggtggcgatc gcggacgacg
     5761 tgggatcggc cgcgccgacg cccgctcccg gcacgctcgc cgcccgcgac ggccggcttc
     5821 tctacggcgc cgccgacggc ccgctcgagt tgctcgaggt gcagcccgcc ggtggcaggc
     5881 cgatggacgc cgccgactgg ctgcgcggcc acggcgccgc gctcgcggcc ggctgagctc
     5941 gccagtccgg ccgacccgcc agaatcctgc gatggccgga gaggagcgaa tcgacctggg
     6001 gccaccgtgt ccgtcgtgcg gcgagccctg gctgcgcgcg acgaacctgc ccggccgcta
     6061 ccgctgcgtc tactgcctga gccgattcga actgcgctcg cagtgccccg actgcggctc
     6121 gcacgcgacg atcgcgcgca tgtccgacct cgtcaacgtc agctgccgca actgcggcgg
     6181 ctcgctgctg acgggcgtct agccgctcag gccgacagct gctcgagcgc ctgcctgatg
     6241 tcggcggcgg tctggaagtc gaggccgagc gcgtggtcgt ggcggtgtcg cacggtgccc
     6301 tgctcgtcga cgatcaccac ggcgcggcgc gtgccgcgca gcttcgacca gcagtcgtag
     6361 gacttggcca ccgactggtc ggggtccgcg agcagcggca cggtcagccc gtgcttgtcg
     6421 gcgaaggcct gcttggagtc gaggcccttg ccggatatcc cgacgacggt cgccgcgagc
     6481 gctccgaacg tctccgcgtt gtcgcggtag gagcagaact ggcgcgtaca gaccggtgtg
     6541 tcgtcgcccg gatagaacag cagcacgacc ttctcgccac ggtggtcgga gagtgtgaac
     6601 ggccccgccg tgccgacgag cgtgaagtcg ggcgccggct cgccgaccgc tgggcgtctg
     6661 ctcatccgct ggaccctagc cgtggcgagg gttacgagtc gcccgtgcgg cggtagagcg
     6721 cgtagagcag cgcgcagagc acgagcgccg cgccggcata gccgacctcg acttggaaga
     6781 cgcggtcgtc gaagcctggc agcagcagga agccgacgta ggccgcggcc gcggccagcc
     6841 agcagacgca ggccgcgcga gcgcgggcgc gcgcgagcag cgcctggttg agcgtcgcgg
     6901 cggcgaggta gaggcccatg ccgagcgaga tcagcaccag gccgatccgg tcgtaggcga
     6961 gctcgccgcc gaagacgagg tccatcagcc acgggccgat cgccagcatg acgagcgcca
     7021 cagcgctcgc gaagagcgcg atcgccagca gggtgacgtt gacgctgcgt cggaacgggt
     7081 cgctctcgcc gccggcgcgc aggcgggtga ggtgggggag gatcgaggtc tggatcgcct
     7141 ggaagagctg cagcggcgcg cgcgcgatca gcagcacgtt gaacgtgaag ccggcgagtg
     7201 cggcgccgtc ggggccgtcg gtcgtcgcct tgacgatcag cgggccggcg ttgaggaagg
     7261 tctgctcggc gagcatgatc gccagcaccg cgaccgcgaa cccggccccg tgcgacaggg
     7321 tgaactccgg ctcaccgccc ggctcgccgg ccgccgcctc gtcgagccgg cgcgcgtcgg
     7381 cggcctccgc gtcggtcagc ccgccggcga tccgccgccc gagtgcccac ggcacgacgg
     7441 ccagcgacgc gatcggcgcc gcggcgatcc cgagcgccac gaccgactgc cccgatagga
     7501 ccccgacggc gaccgcgagc gcgaacaggc agcgcgagac ggcctccatg aagacgaggc
     7561 cgccgtagag gccgaagcgg tgcgagcccg cgaggaagcc gcgcgcgaag tagctcaccg
     7621 cgtaggcgag cacggtcacg atcagcaccc agtagagcgt ctccgagccg tcgaagaggt
     7681 cgtcctgcag cgggccgcgc agcgccagcg ccgcgaccgc gaaggcgatc cccagcccga
     7741 gctggatcgt cgcggccacg cgtagatggc tgcggccggt ggcgctgcgt gccgcgcggt
     7801 cggcgatcgt ccgcgacagc agctgctcga ccgggcggta gagcaccgag accgtgacga
     7861 agacagcgga ccagagcagg gtgatcgcgc cgtagtcgga ctccgacagc gagtggctgg
     7921 cgagtgcgaa gtacgcgtag gtgatcagcc cggtgacgcc gatccccaca gagaggaccg
     7981 ccgcgccctt cccatagctg ccgtcgcccc cggcggctgt gggcatctaa agccttacga
     8041 gcaacatcgt ccaggggaaa ggcgagcgcg cctcgacgac ctcgccgtgg cgcgacagca
     8101 gctcgacgaa gccccgcttc gaccagtggt tgacgtgtcc cggcgtgttg ccgagcgacc
     8161 cccagtaggc gccgcgggcc atgttcagcc cgcgccagag cggctcgcgc ggcaccgaga
     8221 ccaacaggtg gcggctcgcg acgcgcgcca tctcggcgac cgtcgcctcc ggctcgggca
     8281 cgtgctcgag cacctcgatc gccgtcgcca tgtcgaactc gccgtcggcg aacgacagcc
     8341 gcgtggcctc ctcacagcgg tactcgaggt tcgcccgccg gcgcttgtcc cactcggcgc
     8401 gcagcttcgc gtcgtcgagg tcgatcccga cgatccggcc gtcgccgagc cgctgcgccc
     8461 actcgtgggt cagcacgccc tcgccgcagc cgacgtcaag caccgaggtc ggagccgcgc
     8521 ggtcccagag ctcgtcgagc gtgtggtgga aggcggtcat cagccgccgc acgaccgggt
     8581 tggtcgagcc gtacttgtcg aaggtgttgc cggtcgggac cgcctcggcg cccgtcgacc
     8641 ccggtgtcgc gacgccggtc acgcctcgac ggtctcccgg tcggatttgc cggtggcgcg
     8701 cccggccgcc gcgccggtgg tcgccggctg cgcgctgtcc ccggcgcccg gctcgtagtg
     8761 cgacggctcc acgccgagcg tcagctcgac gcggcgcacc cgctcgagtg tgcgctgctg
     8821 cagcgtgcgg ctagcggcga ggacgtcggc gatcacgccg agcgcgacga actggaccgc
     8881 gatgatcagc agcgtcgagc cgaggatcag cgactggacg tggccgttgc cctcgccgtt
     8941 gaagaagaag aagaggaagc gcgcccagat cacggccgcc ggcacggcga cgatcccggc
     9001 cgcgatcagg aagacccgca gcggctcgta gagcgtgtag acgcggaaga tcgccaccgc
     9061 gttgcggcgc acgtactgcg acatcgacgg aaagagccgc gactcgcgcg tcttctcgtt
     9121 ggtgcggatc gggacgtggt cgacggcgac gagcaccttg cctgcctgga tgatcgtctc
     9181 gagcgtgtag gtgaacttcg agatcacctg catctgcagc gccgcctcgc ggttgtaggc
     9241 gcggaagccg gaggtcgtgt cgggcacggt cgtcccggac gcgcgccgca cgaccgcgct
     9301 gccgaggcgc tggaggcgct tcttcagcgg cgagaagtgc tcgatcgagt cggtctcgcg
     9361 atcaccgacg accatgtcgg cgcggcccgc gaggatcggt tcgacgagct tcgggatgtc
     9421 gccgccgtgg tactggttgt cggcgtcggt gttgacgatc aggtcggcgc cgagcttcag
     9481 gcaggcgtcg aggcccgcct ggaacccggc ggcgagtccc ttgttgttgg tcagccgcac
     9541 tacgtggtcg acgccgtgtg cgcgcgccac ctcgaccgtc gcatcggtcg agccgtcgtc
     9601 gatgatcagc cactcgacct cgtcgacgcc aggcacggcg cgcggcaggt cggcgagggt
     9661 cgccggcagc tgctcggcct cgttgaagca cgggatctgg atgatcagct tcatctggga
     9721 gggggagggt agaggcatcg gacgtcgaat tgcagggatc ggcgggcagg cctttgctag
     9781 cctgccccgt tctcgccgta cccctcttcg actggagccg ccgaatgcat cttctcgcat
     9841 acttggatcc cggcagcggc agcatgatcc tgcagatcct cgccggcggg gtcgccgcgg
     9901 tcgctgtcac cgccaagctg tactggagcc gcctgctcaa attgctgcgc atccgcaagg
     9961 acgacgagcc gactcccgag cagcaggccg actcagcctg agccgctgtc cacgtcgact
    10021 gagatatccc cgcgtccgga ggacgcgcag ctagaggccg gctcgttccg cgaccccgag
    10081 agccggatct tctactccgg cggcgacgtc taccgggccc tctcgcagga gggcctcgac
    10141 gacttcgagg cgctccaggc gaccaagctc ttcgagcgct tccaggccga cggccggctc
    10201 gtgcgcacgg agctgctgcc gagcaccgcc gacgtgccga gcgagctgct cgtcaaggac
    10261 tgcgccggcg tgctgcgcca cgagcggatc ccgttcgtct cctaccctta cgagtggacg
    10321 ttctcgatgc tcaaggaggc cgcgctgctg cagctcgacc tcaatctggc ggcgctcgag
    10381 gaggggatga tcctcaagga ctcgacgccc tacaacgtcc agttcaaggg ctcgaagccg
    10441 gtcttcgtcg acgtcggctc gttcgagcgg ctgcgcgacg gcgagccgtg ggtcggctac
    10501 cgccagttct gcatgatgta cctctacccg ctgatgctgc aggcggtgaa ggggatcgag
    10561 ttccatccct ggctgcgcgg ctcgatcggc ggcatcacgc cggcccagat gcgcggcatg
    10621 gtctccttcc gcgaccgctt ccgccgcggc ttcttcctca acgtcttcat gcacgcgaag
    10681 ctcgaggcgc gccacgccga ccgcggcagg gaggtcaagg ccgaggtcaa gcaggccggc
    10741 ttcaagaagg agctgatcgt cgccaacgtg cgccgcatgc gcaagctagt cgagagcctg
    10801 acgtggaacc cgccgaaggg cgtctgggtc gcctacggcg agcgcaacag ctactccgac
    10861 gaggacgcca agcgcaagga cgagttcgtg cgcgaggtgg tcacggccca gccctggagg
    10921 ctgacctggg acatcggcgc caacaacggc cgctactcgc ggatcgccga ggagggctcg
    10981 gactacgtgc tcgccgtcga cgccgaccag ggtccggtcg agctgctcca tcgcacgctg
    11041 cgcgaggagg gcgacgagcg gatcctgacg ctgacgatga acctcgccga cccctcgccg
    11101 agcctcggct ggcgcggcct cgagcgcaag gacctgccgg cgcgcggcaa gcccgacctc
    11161 gtgctcgcgc tcgcgctgat ccatcacgtg acgatcgccg ccaacgtccc ggtcgccgag
    11221 ttcgtcgact ggctcgcatc gctgggaacg tcgctggtga tcgagttccc gacgcgcgag
    11281 gacccgatgg tcaagaagct gctcggaccg aagcgcgagg gactgcatcc cgactacgag
    11341 cgcgtcaact tcgagcgcgt gctcgcgggc gccttcgacg tcgagcgccg tgagcgctcg
    11401 aagtccggca cccgcctgct ctacttcgcg cgtccgaagg gcgccggcag tccgatcg
//
LOCUS       DATABV010000190         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_623060, whole genome shotgun sequence.
ACCESSION   DATABV010000190 DATABV010000000
VERSION     DATABV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623060"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VHF88_08630"
     CDS             <1..571
                     /locus_tag="VHF88_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2161871.1"
                     /translation="MGGMDEAVAAAREIAAARAPAFIPDQFSNPANPEIHRRTTAEEI
                     WRDMDGKVDVLVAGVGTGGTITGCGERLKERNAQLHVVAVEPASSPVISGGRPGPHRI
                     QGIGAGFVPAVLNRAVIDEVVAVSDEDAVAMARNCAVREGVLAGISGGAALWAAIEVG
                     SRPEMTGKRVVVIVPDSGERYVSAPFFAP"
     gene            609..1214
                     /gene="cysE"
                     /locus_tag="VHF88_08635"
                     /pseudo
     CDS             609..1214
                     /gene="cysE"
                     /locus_tag="VHF88_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
     gene            complement(1340..2212)
                     /locus_tag="VHF88_08640"
     CDS             complement(1340..2212)
                     /locus_tag="VHF88_08640"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEX2161872.1"
                     /translation="MGLCDEVRRSCAAVARDARDVTIDLGRVGSIGPGPEPELDRDRH
                     YLEGSAEDVALFTLTLDAINFGSGWFPTLRKRPGCSGYYTVASALTDRFRSDGPWSPR
                     ELAALDADAVADVLRQERGHELMALYAEALRQLGAFLGGRAALDVVAAADGSAERLAE
                     QLVAGIPLFADPGFYKRAQIVASDLTLAGVAEFDDVDRLTIFADNLVPHVLRVDGVLR
                     YSAELAAAIDAGELLPAGREEREIRACAVVACEAIAGDLGVPPRTLDNWLWNRGQEPR
                     YKAIPRHRTRTTAY"
     gene            2559..2954
                     /locus_tag="VHF88_08645"
     CDS             2559..2954
                     /locus_tag="VHF88_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2161873.1"
                     /translation="MSAPLGRAVADTSIFIAREAGRELGPLPDEIAVSAITAGELELG
                     VLRAANAAIRGIRLSTLAEVRGTYPLLEIDALTASCYARIADGELAAGRRVKQHDTWI
                     AATAMRHGVAVLTQDDDFASFDQVEVIRV"
     gene            2995..>5101
                     /locus_tag="VHF88_08650"
     CDS             2995..>5101
                     /locus_tag="VHF88_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX2161874.1"
                     /translation="MRATAQGSEQETAESRVAGLLEGLNEPQREAVTHGEGPLLILAG
                     AGSGKTRALTRRIAYLVGTKQARASEILAITFTNKAAAEMRERVQQLVGNQARAMWVM
                     TFHSACARILRADAEKLNYTRGFTIYDEADSVRLVKQCIEELGVDPKRYAPRAIKRQI
                     SDAKSQLLDAAAYKLKVGGYFEQTVAEVYERYERRIHAMNAMDFDDLLLQSVRLFEMF
                     PEVLERYRRSFRHVLVDEYQDTNNAQYRWLRLLADEHRNLAVVGDDSQAIYSFRGADI
                     RNILDFEHDFPDANVIKLEQNYRSTQAILDASNAVIANNRRQKEKHLWTDQDGGDPVR
                     VRELEDEHAEARYVAGEIERHVEGGGSRNDVAVFYRTNAQSRVLEDTLVRYGVSYQVI
                     GGTKFYERAEVKDALAYLTLLTNPQDVVSFGRIVNSPRRGIGQTSQARIVAHANTIGE
                     PIFDVAATPEDVPGLGAAAIKAVGRFMETMEKLRDRADGARVGDLLKDVLHEVGYFDA
                     LERERTIEAQGRLENLEELVGVAREYDAARGGALGGVEDDGGGQEPSVEEFLQQVALF
                     SQQDELQDDEGVVTLMTLHNAKGLEYDVVFMIGCEDGVFPHSRSIEAGDVEEERRLCY
                     VGMTRARKQLTLTHARTRALYGGREWNVPSRFLAEIPLELTDAEEQVGARERATTWGA
                     SVGGTPGIGGPGSTWESLRR"
BASE COUNT          807 a         1770 c         1830 g          694 t
ORIGIN      
        1 gatgggcggg atggacgagg cggtcgccgc cgcgcgcgag atcgccgccg cgcgcgcacc
       61 ggcgttcatc cccgaccagt tctcgaatcc cgccaacccg gagatccacc gccgcacgac
      121 cgccgaggag atctggcgcg atatggacgg caaggtcgac gtcctggtcg cgggcgtcgg
      181 gacgggcggg acgatcaccg gctgcggcga gcgtctgaag gagcgcaatg cccagctcca
      241 cgtcgtggcc gtcgagcccg ccagctcgcc ggtgatctcc ggcggccggc cggggccgca
      301 tcggatccag gggatcggcg cggggttcgt gccggcagtg ctgaaccgcg cggtcatcga
      361 cgaggtggtc gcggtgtccg acgaggatgc ggtcgcgatg gcgcgcaact gcgccgtccg
      421 ggagggcgtg ctcgccggca tctccggcgg agcagcgctg tgggcggcga tcgaggtcgg
      481 ctcacgaccg gagatgaccg ggaagcgggt cgtcgtaatc gtgcccgact ccggcgagcg
      541 ctacgtcagc gcccctttct tcgccccgta acctccctct cgtggtcgga cttgacacat
      601 tgacgcgggt gacgcgggag gtgcggcagg acctcgccgc cgcgcatgcg cgagacccgg
      661 cttcgcacga cgtcgggcgg ctggagatcc tgctcaccta cggcggggtc caggcgctgc
      721 tgtcgcaccg gatcgcccac gcgctgcacc aggccggcgt gccgatcgtg ccgcgcttcc
      781 tggcgaacct gaccaaggtc atcaccggcg tcgagatcca cccggcggcg cggatcggcg
      841 aagcgctgtt catcgaccac ggggcgggtg tggtgatcgg cgagacggcc gagatcggcg
      901 agaacgtcac ggtctaccaa ggcgtgacgc tcggcggcac cggcttcgag gtcggcaagc
      961 gccatccgac cgtcggcgac gacgtcgtca tcggctccgg ggccaagctg ctcggcccga
     1021 tcgtcgtcgg cgaccgggcg aagatcggcg ccaactccgt agtcatccac gacgtcccgc
     1081 gcgactcgac agtcgtcggc aaccccggtc atccggtccg catcgacggc gagcggccgg
     1141 agggcccgga cgccgattgg gcgcacctgc cggatccggt cgccgacgcg ctcaagggca
     1201 tggccgagcg gatcggcgag ctcgaggagc tcgtcgccga gctgaccggc acgcagcgcg
     1261 agggcgtggc cgaggtccgt ccgttgcggc gcggtgcggg caacgacccg gcgtcgggct
     1321 gagccgcgcc gcgctgcagt cagtaggcgg tcgtccgcgt ccggtggcgc gggatcgcct
     1381 tgtagcgcgg ctcctggccg cggttccaca gccagttgtc aagcgtgcgc ggcgggacgc
     1441 cgaggtcgcc ggcgatcgcc tcgcaggcga cgaccgcgca ggcgcggatc tcgcgctcct
     1501 ctcgcccggc cggcaacagc tcgccggcgt cgatcgcagc ggccagctcc gccgagtagc
     1561 gcagcacccc gtcgacgcgc agtacgtgcg gcacgaggtt gtcggcgaag atcgtcaggc
     1621 ggtcgacgtc gtcgaactcg gcgacgccgg ccagagtcag gtcgctcgcg acgatctgcg
     1681 cgcgcttgta gaagccggga tcggcgaaca gcggtatgcc ggcgacgagc tgctccgcca
     1741 gccgctcggc cgacccgtcc gccgcggcga ctacgtccag tgcggcgcgc ccgcccagga
     1801 aagcgccgag ctggcgcagc gcctctgcgt acagcgccat cagttcatgc ccgcgctcct
     1861 ggcggagcac gtccgcgaca gcgtcggcgt cgagcgccgc cagctcgcgc ggcgaccacg
     1921 gcccgtcgct ccggaaccgg tcggtcagcg ccgaggcgac cgtgtagtag cccgagcacc
     1981 cgggccgctt gcgcagggtc ggaaaccagc ccgagccgaa gttgatcgcg tcgagggtca
     2041 gcgtgaacag cgcgacgtcc tccgccgacc cctcgaggta gtgccggtca cgatcgagct
     2101 ccggctccgg tcccggcccg atcgagccga cacgacccag atcgatcgtc acgtctcgcg
     2161 cgtcccgcgc caccgccgcg cagctgcgcc gaacctcgtc gcagagcccc atcggcgcga
     2221 ggctattcac tcgccgattc aggacgctca gcagcggctg caaccggccc gtagcaagcc
     2281 tgtagcacgc gttgctacgc tgtcgcaatg gcacggtcag tctccgtacg agagttaagg
     2341 aacaacactg cggaggtggt agctgccgtg caagccggag agacgctcac gctcaccgtg
     2401 aaccagacgc ccgtggcgga cgtcgtgcct catcgcccgc atcggagccc atgggtgccg
     2461 tcgtcggagc tgcgccggat cgtcgcggag gcgccagccg acccgggtct gttcgaagac
     2521 ctcgccgagg tgcgcagcga gctcttggac gaccctcggt gagcgctccc ctcggcaggg
     2581 cggtagcgga cacttccatc ttcatcgcca gggaggcagg ccgtgagctc ggcccactgc
     2641 cggatgagat cgccgtgtcg gcgataaccg ccggcgagct cgagctcgga gtgctgcgcg
     2701 ccgccaacgc ggcgatacgc ggcatcagac tgtctacttt ggccgaggtt cgcggtacgt
     2761 acccgttgct ggagatcgac gcgctgactg cgtcgtgcta cgcgcggatc gcggacggcg
     2821 agctggcggc gggtcgcagg gtcaagcagc acgacacgtg gatcgccgcg acggcgatgc
     2881 gtcatggcgt cgccgtcctg actcaggacg acgacttcgc gtccttcgac caggtcgagg
     2941 tcatccgggt ctgagcgagt ccggccggcc gcgaccggcc ctatcctcag tcgggtgcgc
     3001 gcgacggccc agggatcgga gcaggagacc gccgagagcc gggtcgccgg gctcttggag
     3061 gggctcaacg agccgcaacg cgaggcggtc acccacggcg aggggccgct gctgatcctc
     3121 gccggcgcgg gctcgggaaa gacgcgcgcg ctgacgcggc ggatcgccta cctggtcggc
     3181 acgaagcagg cccgcgcaag cgagatcctg gcgatcacgt tcaccaacaa ggccgccgcc
     3241 gagatgcgcg agcgcgtcca gcagctcgtc ggcaaccagg cgcgcgcgat gtgggtgatg
     3301 accttccact cggcctgcgc gcggatcctg cgcgcggacg ccgagaagct caactacacg
     3361 cgcgggttca cgatctacga cgaggccgac tcggtccgcc tcgtcaagca gtgcatcgag
     3421 gagcttggcg tcgatcccaa gcgctacgcg ccgcgggcga tcaagcgcca gatctccgac
     3481 gccaagagcc agctgctcga cgcggcggcc tacaagctga aggtcggcgg ctacttcgag
     3541 cagaccgtcg ccgaggtcta cgagcgctac gagcggcgga tccacgcgat gaacgcgatg
     3601 gacttcgacg acctgctgct gcagtcggtg cgcctgttcg agatgttccc cgaggtgctc
     3661 gagcgctacc gccgctcctt tcgccacgtc ctcgtcgacg agtaccagga caccaacaac
     3721 gcccagtacc gctggctgcg cctgctcgcc gacgagcacc gcaacctcgc agtagtaggg
     3781 gacgactcgc aggcgatcta cagcttccgc ggcgctgaca tccgcaatat cctcgacttc
     3841 gagcacgact tccccgacgc gaacgtgatc aagctcgagc agaactaccg ctcgacgcag
     3901 gcgattctcg atgcctcgaa cgcggtgatc gccaacaacc gccgccagaa ggagaagcat
     3961 ctctggaccg accaagacgg cggcgatccg gtccgcgtcc gcgagctcga ggacgagcac
     4021 gccgaggcgc gctacgtcgc cggcgagatc gagcgccacg tcgagggcgg cggctcgcgc
     4081 aacgacgtcg cagtcttcta ccggaccaac gcgcagagcc gcgtgctcga ggacacgctg
     4141 gtccgctacg gggtcagcta tcaggtgatc ggcgggacga agttctacga gcgcgccgag
     4201 gtcaaggacg cgctcgccta cctgacgctg ctgaccaacc cgcaggacgt cgtctcgttc
     4261 gggcggatcg tcaactcgcc gcgacgcggg atcggtcaga cgtcgcaggc gcggatcgtc
     4321 gcccacgcga acacgatcgg cgagccgatc ttcgacgtcg cggcgacgcc cgaggacgtc
     4381 ccgggcctcg gcgcggcagc gatcaaggcc gttggccgct tcatggagac gatggagaag
     4441 ctgcgcgacc gcgccgacgg ggcgcgcgtc ggcgacctgc tcaaagacgt actgcacgag
     4501 gtcggctact tcgatgcgct cgagcgggag cggacgatcg aggcgcaggg ccggctcgag
     4561 aacctcgagg agctggtcgg cgtcgcgcgc gagtacgacg ctgcgcgcgg cggcgcgctc
     4621 ggcggagtcg aggacgacgg gggcggccag gagccgtcgg tcgaggagtt cctgcagcag
     4681 gtcgcgctgt tctcgcagca ggacgagctg caggacgacg agggagtcgt cacgctgatg
     4741 accctccaca acgccaaggg cctcgagtac gacgtggtct tcatgatcgg ctgcgaggac
     4801 ggcgtcttcc cgcactcgcg ctcgatcgag gccggcgacg tcgaggagga gcgccgcctc
     4861 tgctacgtcg ggatgacgcg cgcgcgcaag cagctgacgc tgacccacgc gcgcacgcgc
     4921 gccctctacg gcggccgcga gtggaacgtc ccgagccgct tcctcgccga gatcccgctc
     4981 gagctgaccg acgcggagga gcaggtgggg gcgcgggagc gggcgaccac ctggggcgcg
     5041 agcgttggtg gtacgccggg gattgggggt cccggctcca cttgggaatc gttgcgccgg
     5101 g
//
LOCUS       DATABV010000191         8885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1747227, whole genome shotgun sequence.
ACCESSION   DATABV010000191 DATABV010000000
VERSION     DATABV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747227"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VHF88_08655"
     CDS             complement(<1..1184)
                     /locus_tag="VHF88_08655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2161875.1"
                     /translation="MRSAIVRPVCRIPRVVYDAMVRVGIVSPYSYTYPGGVGRHAEAL
                     TAELTRQGHEVRLLAPYDPDDRLARVMHRGAAPEQRPLPEYLIPLGRTAGLPMNGAVS
                     NLALSPAVVPALHRELRAGDFDVVHVHEPNAPFVSWFATELARTPLVGTFHAYSRSPV
                     ANGLAANVATARRLYNKLHVRIAVSEAARWTCERFYGGEYRIVPNGVDLSAARPTDRS
                     LPREQLELLFLGRAEARKGLPVLLRAFEALRGAGVAARLTVAGATPAEVEPLLLDLEG
                     VEVAGRVDDDEKQRLLGAADLLVAPALGGESFGMVLTEAFAAGTPAVASDIAGYRDVA
                     RDGIDSVLVPPADAAALGEALRELALDPQRRLQMGAAARERAERFSWSHVAEEVVEAY
                     EDA"
     gene            1171..1869
                     /locus_tag="VHF88_08660"
     CDS             1171..1869
                     /locus_tag="VHF88_08660"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX2161876.1"
                     /translation="MAERNYLKYSFVKVSPEWRRRSAQERADDKREFAAACAEFATDH
                     YLRAYSLVGTRGDCDLMIRAVAPTLDPIHELHVLLNQSGLMRFADIRHSYLALTKESV
                     YSDEEQPTAPRPGSDRRYFIVYPMWKKREWYALPPDERMRIMRGHIETGKRYPGIEIN
                     TAYSYGLDDQEFVVSFNADDPAEFLDLVQELRSTESSAYTASETPIFTCISASPERAL
                     DALDGTAVTAALTG"
     gene            complement(1866..3260)
                     /locus_tag="VHF88_08665"
     CDS             complement(1866..3260)
                     /locus_tag="VHF88_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2161877.1"
                     /translation="MSLGTFDNPLRVAVVGSGPAGFYSAGHLLADKELTIEVDMFDRL
                     PTPFGLVRAGVAPDHPKIKSVTRVYEKTAAKEQFRFFGNVKVGEHLSHEDLARHYHAI
                     VYAYGCETDRQLGIDGEDLPGSYAATEFVAWYNGHPDYRDLEFDLSCENVAIVGNGNV
                     AMDVARMLVLPRAELEITDTADHAIEALANSNVRHVYILGRRGPAQAAFTNPELRELG
                     ELTDADVIVEPFELDRHSAQSIEGEGDITPRRNVEILQDFAAREPEGKPKRVELRFLV
                     SPVAIRGEGRVEQIELVHNKLEHGAEGRIRAQATDVHETLDVGLVFRSIGYRGIPIEG
                     VPFDEWKGTIPNEEGRVLDAHEQRAIPGLYVAGWVKRGPSGVIGTNKRDAQETVEHLL
                     EDVREGRLEQPERPARAAIEELVHERQPDVVDYAGWEAIDVAEQAAGEPQGRPRVKLT
                     RVAEMVEAARAARV"
     gene            3286..4857
                     /locus_tag="VHF88_08670"
     CDS             3286..4857
                     /locus_tag="VHF88_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX2161878.1"
                     /translation="MNRTAIIDMGSNSFRLVVFQHEPGGWWSLADEIREPVRVSAGMG
                     DEGVLAPEPIDRAVATAAVFASFLEASGVERVDAVATSAIRDARNRDELLGAIRGRTG
                     LEVRVISGAEEAWYGYLAIVNSTTLADGFGIDIGGGSVQVMQVADRRLVEAESVRLGA
                     VRVSEAFLPGERAKPKQAQGLRDHVARTLSEFEWWGQTGGSRPRTPEAPGRSPDGAGR
                     LVAIGGTVRNLATAIQKRMELPDVDEQGFVVTREALEELIELLAERPASERGAVPGIK
                     PDRGDVILGGALVVAEAMEQGGFTELEVAEAGLREGVFFEHLLADRSPPLIRDVRTAS
                     VENLAERFQTDPRHVAHVAKLSLELFDALREAGLHQLGDNDRQVLWAAGLLHDVGMTV
                     DYDDHHRHSHYLIVHAGLPGYSPRELDLIALIARYHRKGTPDPSELGALRRDGDRERL
                     LILCGLIRLAEQLERSRDQAVASVRAHIGDEHLVLEALPRPGADPAVAIWAARRGSDL
                     LTEALGRRLEIRPSR"
     gene            complement(4861..5241)
                     /locus_tag="VHF88_08675"
     CDS             complement(4861..5241)
                     /locus_tag="VHF88_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161879.1"
                     /translation="MDTGLLIVLIVAAVVLVALLVMAMRKARARRDEQHRVEAGEHRE
                     EARIRGARADRQQAEAEERAARARAELAGAEEQATVAGRERRAAEERHEHAAQLDPDV
                     DENEAGDLPRADQIRHGDERGEQR"
     gene            complement(5334..5954)
                     /locus_tag="VHF88_08680"
     CDS             complement(5334..5954)
                     /locus_tag="VHF88_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2161880.1"
                     /translation="MTVDAFEHLAACDPVLGALVSGQGPLDEEGRRRGRPDDAYGALL
                     RAIVGQQLSVKAARTIYDRLCALYGDRTPTPAELIATDPDQLRAVGLSGAKAAYLRDL
                     AEHVEDGRLDLERLPELPDDDVSAQLIAIKGLGRWTVDMFLMFHLRRPDVLPVGDLGI
                     RRAVQIAYGLDDLPDAAALTRIAEPWQPHRTLACLYLWASLDNAPD"
     gene            complement(5951..7345)
                     /locus_tag="VHF88_08685"
     CDS             complement(5951..7345)
                     /locus_tag="VHF88_08685"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX2161881.1"
                     /translation="MPTGRIRRTAKVAGLAGGQTARNYATKAANLTRGEQQRREAAAR
                     RQAEAAEQILDVLGNMKGAAMKVGQVASFIDTGAFPEEFQARIQEKLAELRDSAPRVP
                     FKQMRAVIEDGLGESIDDVFAEFDERAVAAASIGQVYRARLGDDAGRLAGREVAVKVQ
                     YPGVARAVRADLQNLGLLMRVAKRIAPGMDAKAMTAEIRERLSDELDYEQEAQAQRAM
                     ARAWRGHPFVVIPEVVTSLSSERVLVTEWLDGRSFEEVRELPQSERDRFGEIVYRFFC
                     GSLYRNGHFSGDPHPGNYRLMNDGRVAFLDFGMTKHVDRDYLERELAVLRAGMAGDAD
                     ALLAALVAAGYYAADDPLVSPEAVLAHVRAIAWWYMEDRPVTIDRELVTQVLIDAGDP
                     RSRHWDLIKRGTVPAEAILARRMEGLVVGVLGHLGVTANWHRIACEWLFDDPPSTPLG
                     ELEADYFGERSAAA"
     gene            complement(7377..7733)
                     /locus_tag="VHF88_08690"
     CDS             complement(7377..7733)
                     /locus_tag="VHF88_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX2161882.1"
                     /translation="MVTLSDIAADKVRGFLAQQQTDGDVGLRIGVRGGGCSGFQYALA
                     LDERRDDDNVFEHGGIPILVDSASLRYVDGSEVDYTESMMGAGFEVNNPNVVASCGCG
                     SSFRIADEDPSCGSAA"
     gene            7806..8045
                     /locus_tag="VHF88_08695"
     CDS             7806..8045
                     /locus_tag="VHF88_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX2161883.1"
                     /translation="MPTSDELKQRIEAALPGARVTVEDLTGGGDHFRAEVESDSFEGL
                     NRIQQHKLVYDVFGAEVGGPIHALSIKTSTPGGTP"
     gene            8042..8443
                     /gene="grxD"
                     /locus_tag="VHF88_08700"
     CDS             8042..8443
                     /gene="grxD"
                     /locus_tag="VHF88_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX2161884.1"
                     /translation="MTNTEIREFIENAIAENPVMLFMKGSPEQPACGFSSRTAACLNA
                     LGVRYAALDILPDPRIREELSGLSGWPTIPQLFVKGELVGGADIVTEMYESGELAELL
                     EAEQPEDAEDVAAQSEAPVGNGPLPLENRLD"
     gene            8502..8822
                     /locus_tag="VHF88_08705"
     CDS             8502..8822
                     /locus_tag="VHF88_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2161885.1"
                     /translation="MPVSVVTDTTGYLPREHLDRYGITAVPLYVVFADRTVKETEITD
                     YPAFFEELRRADSVPKTSQPSVGDFVAAFEPLLARGDEIASIHISGGISGTVAFQLLG
                     RLAR"
BASE COUNT         1261 a         3218 c         3115 g         1291 t
ORIGIN      
        1 gcgtcctcgt aggcctcgac gacctcctcg gcgacgtgcg accaggagaa gcgctcggcg
       61 cgctctcgcg cggcggcgcc catctggagg cgtcgctgcg ggtcgagtgc cagctcacgc
      121 aacgcctcgc cgagtgccgc cgcgtccgcc ggcgggacga gcacgctgtc gatcccgtct
      181 cgcgccacgt cgcggtagcc ggcgatgtcc gaggccacgg ccggcgtccc ggcggcgaac
      241 gcctcggtca gcaccatgcc gaagctctcg ccaccgagcg ctggcgccac gagcaggtca
      301 gcggcgccga gcaggcgctg cttctcgtcg tcgtcgacgc ggccggcgac ctccacccct
      361 tcgaggtcga gcagcagcgg ctcgacctca gccggcgtcg cgccggcgac ggtcaggcgg
      421 gcggcgacgc ccgcgccgcg cagcgcctcg aaggcgcgca gcagcaccgg caggcccttg
      481 cgcgcctcgg cgcggccgag gaacagcagc tcgagctgct cgcgcggcag cgagcggtcc
      541 gtgggccggg cggccgacag atcgacgccg ttcgggacga tcctgtactc gccgccgtag
      601 aagcgctcgc aggtccagcg cgccgcctcc gagaccgcga tccgcacgtg cagcttgttg
      661 tagagccggc gcgcggtcgc gacgttcgcg gcgaggccgt tcgcgaccgg gctgcgcgag
      721 taggcgtgga aggtgccgac cagcggcgtg cgcgccagct cggtggcgaa ccagctgacg
      781 aagggcgcgt tgggctcgtg gacgtggacg acgtcgaagt cgcccgcacg cagctcgcgg
      841 tgcagcgccg gcacgaccgc cggcgacagc gcgaggttcg acaccgcgcc gttcatcggc
      901 aggcccgcgg tgcgcccgag cgggatcagg tactcgggca ggggccgctg ctcgggtgcg
      961 gcgccccgat gcatcacacg tgccaggcgg tcgtccgggt cgtacggggc gaggaggcgg
     1021 acctcgtgcc cctggcgcgt cagctccgct gtcagagcct ccgcgtgccg gccgactccg
     1081 ccgggatacg tgtacgagta gggggagacg atcccgacgc gcaccatcgc atcgtagacg
     1141 acgcgtggga tgcggcagac tgggcggacg atggccgagc gcaactacct caagtacagc
     1201 ttcgtgaagg tctccccgga atggcgacgg cgcagtgcgc aagagcgcgc ggacgacaag
     1261 cgggagttcg ccgccgcgtg cgccgagttc gccaccgacc actacctgcg cgcctactcg
     1321 ctggtcggca cgcgcggcga ctgcgacctg atgatccgcg ccgtcgcccc cacgctcgac
     1381 ccgatccacg agctgcacgt gctgctcaac caatccgggt tgatgcgctt cgccgacatc
     1441 cgccactcct acctggcttt gacgaaggag tcggtctact cggacgagga gcagccgacc
     1501 gcgccccgcc ccggctcgga ccgtcgctac ttcatcgtct atccgatgtg gaagaagcgc
     1561 gagtggtacg cgctgccgcc cgacgagcgg atgcggatca tgcgcgggca catcgagacc
     1621 ggcaagcgct acccggggat cgagatcaac accgcctact cctacgggct cgacgaccag
     1681 gagttcgtcg tctcgttcaa cgccgacgac cccgccgagt tcctcgacct cgtccaggag
     1741 ctgcgctcga ccgagtcgag cgcctacacc gcctcggaga cgccgatctt cacctgcatc
     1801 agcgccagcc ccgagcgggc gctcgacgcg ctcgacggga ccgccgtcac cgccgcactg
     1861 acgggctaga cacgggcggc gcgcgccgcc tcgaccattt ccgcaactcg ggtcagcttc
     1921 acgcgcggcc tcccctgcgg ctcgccggcg gcctgctcgg cgacgtcgat cgcctcccag
     1981 cccgcgtagt cgacgacgtc gggctggcgc tcgtggacga gctcctcgat cgccgcccgt
     2041 gccggtcgct cgggctgctc gagccggccc tcgcgaacgt cctcgagcag gtgctcgacc
     2101 gtctcctggg cgtcgcgctt gttggtcccg atcaccccgg acggaccgcg cttgacccag
     2161 ccggcgacgt agaggccggg gatcgcgcgc tgctcgtgcg cgtcgagcac gcggccctcc
     2221 tcgttgggga ttgtgccctt ccactcgtcg aacggcacgc cctcgatcgg tatgccgcgg
     2281 tagccgatcg agcggaacac caggccgaca tcgagcgtct cgtggacgtc ggtcgcctgc
     2341 gcgcggatcc ggccctcggc tccgtgctcg agcttgttgt ggaccagctc gatctgctcg
     2401 accctgccct cgccgcggat cgccaccggc gagaccagga agcgcagctc gacccgcttc
     2461 ggcttgccct ccggctcgcg cgccgcgaag tcctgcagga tctcgacgtt gcggcgcggc
     2521 gtgatgtcgc cctcgccctc gatcgactgg gcgctgtggc ggtcgagctc gaacggctcg
     2581 acgatcacgt cggcgtcggt cagctcgccg agctcgcgca gctcggggtt ggtgaaggcc
     2641 gcctgcgccg gcccgcgccg gccgaggatg tagacgtgcc tgacgttcga gttggcgagc
     2701 gcctcgatcg cgtgatcggc cgtgtcagtt atctccagct ccgcgcgcgg cagcacgagc
     2761 atgcgcgcga cgtccatcgc gacgttgccg ttgccgacga tcgccacgtt ctcgcaggag
     2821 aggtcgaact cgaggtcgcg gtagtccggg tggccgttgt accaggcgac gaactcggtg
     2881 gcggcgtagc tgccgggcaa gtcctcgccg tcgatcccga gctggcggtc ggtctcgcag
     2941 ccgtacgcgt agacgatcgc gtgatagtgg cgcgcgaggt cctcgtggct gaggtgctcg
     3001 ccgaccttga cgttgccgaa gaagcggaac tgctccttgg cggcggtctt ctcatagacc
     3061 cgcgtgaccg acttgatctt cggatggtcg ggcgcgacgc ctgcgcggac gaggccgaac
     3121 ggcgtcggca ggcggtcgaa catgtcgacc tcgatcgtca gctccttgtc ggccagcaga
     3181 tggcccgccg agtagaagcc ggcgggcccg gagccgacga cggcgacgcg cagcgggttg
     3241 tcgaacgttc cgagggacac gcggcgcgag atgatagccg cgcccgtgaa ccgcaccgcg
     3301 atcatcgaca tggggtcgaa ctccttccgg ctggtcgtct tccagcacga gccgggcggc
     3361 tggtggagcc tcgccgacga gatccgcgag ccggtgcggg tcagcgccgg gatgggcgac
     3421 gagggcgtcc tggccccgga gccgatcgat cgtgccgtag cgaccgcagc ggtcttcgcc
     3481 tcgttcctcg aggcgtccgg cgttgagcgg gtcgacgccg tcgcgaccag cgcgatccgc
     3541 gacgcgcgca accgcgacga gctgctcggc gcgatccgcg gtcggaccgg gctggaggtg
     3601 cgcgtgatca gcggcgccga ggaggcctgg tacggctacc tggcgatcgt caactcgacg
     3661 acgctcgccg acggcttcgg gatcgacatc ggcggcggca gcgtgcaggt gatgcaggtc
     3721 gctgaccgcc gcctggtcga ggcggagtcg gtgcgactcg gcgcggtgcg ggtcagcgag
     3781 gccttcctgc cgggcgagcg ggcgaagccg aagcaggccc aagggttgcg tgaccacgtc
     3841 gctaggacgc tctctgagtt cgaatggtgg ggtcagaccg ggggctcgcg ccccaggacc
     3901 cccgaggcgc ctggccggtc gcccgatggc gcggggcggc tggtggcgat cggcggaacg
     3961 gtgcgcaacc tcgcgacggc gatccagaag cggatggagc tgccggacgt cgacgagcag
     4021 gggttcgtcg tcacgcgcga agcgctcgag gagctgatcg agctgctggc cgagcggccg
     4081 gcctccgagc gcggagcggt cccgggcatc aagccggacc gcggcgacgt gatcctcggc
     4141 ggcgcgctgg tcgtcgcgga ggcgatggag cagggcggct tcaccgagct cgaggttgcg
     4201 gaggcgggac tgcgcgaggg cgtcttcttc gagcatctgc ttgccgaccg cagcccgccg
     4261 ctgattcgcg acgtgcggac ggcttcggtc gagaacctcg ccgagcgctt ccagacggat
     4321 ccgcgccacg tcgcccatgt ggcgaagctg tcgctcgagc ttttcgacgc gctgcgcgaa
     4381 gccggactgc accagctcgg cgacaacgac cgccaggtcc tatgggccgc cggcctgctc
     4441 cacgacgtcg gcatgacggt cgactacgac gaccaccacc gccactcgca ctacctgatc
     4501 gtccatgccg gcctgcccgg ctacagcccg cgtgagctcg acctgatcgc gctgatcgcc
     4561 cgctaccacc gcaagggcac ccccgacccg agcgagctcg gcgcgctgcg tcgcgacggt
     4621 gaccgcgagc ggctgctgat cctctgcggc ctgatccggc tcgccgagca gctcgagcgc
     4681 agccgcgacc aggcggtcgc ctcggtccgg gcacatatcg gtgacgagca cctcgtgctc
     4741 gaagccctcc cccgtcctgg cgccgacccc gctgttgcta tctgggcggc ccgccgcggc
     4801 tcggacctgc tcaccgaggc gctaggccgg cgcctggaga tccggccgag tcgctagtcg
     4861 ctagcgctgc tcgccgcgct cgtcgccgtg acggatctga tcggcgcgcg gcagatcgcc
     4921 cgcctcgttc tcatcgacgt cgggatctag ctgcgccgcg tgctcgtggc gctcctccgc
     4981 ggcccgtcgc tcgcgtcccg caacggtcgc ctgctcctct gccccggcca gctcggcacg
     5041 ggcacgggcg gcgcgctcct ctgcctccgc ctgctggcga tcggcgcggg cgccgcggat
     5101 gcgggcctcc tcgcggtgct cgcccgcctc gacgcggtgc tgctcgtcgc ggcgcgcacg
     5161 cgccttgcgc atcgccatca ccaagagcgc gaccagtacg acggccgcga ctatcaagac
     5221 gatcaagagc ccggtgtcca tcgcgtactc ctcctctgtg gggtgcctgt gaggtacccg
     5281 atgccgggcg cgcaataacc gcagcagcga gccgacgtcg gttgcgcgct cgctcagtcc
     5341 ggcgcgttgt cgagcgacgc ccacaggtag aggcaggcga gcgtgcggtg cggctgccat
     5401 ggctcggcta tccgcgtcag tgcggcggcg tccgggaggt cgtcgaggcc gtaggcgatc
     5461 tggaccgcgc gacggatccc gaggtcgccg accggcagga cgtcgggcct gcgcagatgg
     5521 aacatcagga acatgtcgac cgtccagcgg ccgagcccct tgatcgcgat cagctgcgct
     5581 gacacgtcgt cgtcgggcag ctccggcagc cgctcgaggt cgagccgccc gtcctcgacg
     5641 tgctcggcga ggtcgcgcag ataggcggcc ttggcacccg agagcccgac ggcgcgcagc
     5701 tggtccgggt cggtcgcgat cagctcggcc ggtgtcggcg tgcggtcgcc gtagagcgcg
     5761 cagaggcggt cgtagatcgt gcgggccgcc ttgaccgaca gctgctgacc gacgatcgcg
     5821 cgcagcagcg caccgtaggc gtcgtcgggc cgcccgcgcc gcctgccctc ctcgtcgagc
     5881 ggcccctggc ccgagacgag cgcgcccagg accgggtcgc aagcggccag gtgctcgaac
     5941 gcgtcgacgg tcatgccgcc gcgctgcgct cgccgaagta gtccgcctcg agctcaccca
     6001 gcggagtcga cggcgggtcg tcgaacagcc actcgcaagc gatccggtgc cagttcgccg
     6061 tcaccccgag gtggccgagg acgccgacga cgagtccctc catccgccgc gcgaggatcg
     6121 cctccgccgg gaccgttccg cgcttgatca agtcccagtg ccgtgagcgc ggatcgccgg
     6181 cgtcgatcag gacctgggtg accaactcgc ggtcgatcgt caccgggcga tcctccatgt
     6241 accaccaggc gatcgcgcgg acgtgggcga gcaccgcctc cggcgagacc agcgggtcgt
     6301 cagccgcgta gtaaccggcc gcgacgagcg ccgcgagcag agcgtccgcg tcgccggcca
     6361 tgccggcgcg cagcacggcc agctcgcgct cgaggtagtc gcggtcgacg tgcttggtca
     6421 tcccgaagtc gaggaacgcg acccgaccgt cgttcatcag gcggtagttg cccgggtggg
     6481 gatcgcctga gaagtggccg ttgcggtaca gcgagccgca gaagaagcgg tagacgatct
     6541 ctccgaagcg gtcgcgctcg gattgcggca gctcgcgcac ctcctcgaac gaccgtccgt
     6601 cgagccactc ggtgaccagg acgcgctcgc tgctcagcga cgtcacgacc tccgggatca
     6661 cgacgaacgg gtgtccacgc caggcacgcg ccatcgcccg ctgcgcctgg gcctcctgct
     6721 cgtagtcgag ctcgtcggag agccgctcgc ggatctccgc ggtcatcgcc ttggcgtcca
     6781 tccccggcgc gatccgcttc gcgacgcgca tcagcagccc gaggttctgg agatcagcgc
     6841 gaaccgcgcg cgcaacgccc gggtactgca ccttgaccgc gacctcgcgg ccggcgagcc
     6901 gtcccgcgtc gtcgcccagc cgtgcgcggt agacctgacc gatcgacgcg gccgcgaccg
     6961 cccgctcgtc gaactcggcg aagacgtcgt cgatcgactc gccgagcccg tcctcgatca
     7021 cggcgcgcat ctgcttgaac ggtacgcgcg gagcggagtc gcgcagctcg gcgagcttct
     7081 cctggatccg tgcctggaac tcctcgggga aggcaccggt gtcgatgaac gacgccacct
     7141 gcccgacctt catcgccgcg cccttcatgt tccccagcac gtcgaggatc tgctcggccg
     7201 cctcggcctg gcggcgggcg gcggcctcgc gccgctgctg ctctccgcgc gtcaggttcg
     7261 cggccttggt ggcgtagttg cgcgccgtct gcccacccgc gagccccgcc accttcgcag
     7321 tgcgccggat ccggccggtc ggcaccgagt tgtcgccctt gcgcggcagg cgcttcctac
     7381 gcggccgagc cgcagctcgg gtcctcgtcc gcgatgcgga acgacgaacc gcagccgcag
     7441 gaggcgacga cgttggggtt gttcacctcg aagccggcgc ccatcatgct ctcggtgtag
     7501 tcgacctcgg agccgtcgac gtagcgcagg ctggccgagt cgacgaggat cgggatgccg
     7561 ccgtgctcga agacgttgtc gtcgtcgcgg cgctcgtcga gcgccagcgc gtactggaag
     7621 ccggagcagc cgccgccacg cacgccgatg cgcagcccga cgtcgccgtc ggtctgctgt
     7681 tgggcgagga atccacgcac cttgtcggcc gcgatgtcgg agagagtcac catgcttcag
     7741 taaatgtagc aatggctccc gagctcaaga gcccaccgct gaccctcgct atggtgccag
     7801 gtcgaatgcc gacgagcgac gagctgaagc aacggatcga ggccgccctc ccgggcgcgc
     7861 gcgtgaccgt cgaggacctc accggaggtg gcgaccactt ccgcgccgag gtcgagtccg
     7921 acagcttcga ggggctcaac cggatccagc agcacaagct cgtctacgac gtgttcggcg
     7981 ccgaggtcgg cggaccgatc cacgccctct cgatcaagac ttccactccc ggaggaaccc
     8041 catgacgaac accgagatcc gcgagttcat cgagaacgcg atcgccgaga acccggtgat
     8101 gctgttcatg aagggcagcc ccgagcagcc ggcctgcggc ttctcctcgc gcaccgccgc
     8161 ctgcctcaac gcgctcggcg tccgctacgc cgcgctcgac atcctgcccg acccgcgcat
     8221 ccgcgaggag ctgtcgggcc tctccggctg gccgacgatc ccgcagctgt tcgtcaaggg
     8281 cgagctggtc ggaggcgccg acatcgtcac cgagatgtac gagtcgggcg agctcgccga
     8341 gctgctcgag gccgagcagc cggaggacgc cgaggacgtc gcggcgcagt cggaggcgcc
     8401 ggtcggcaac ggccccctgc cgctcgagaa ccgcctcgac tgaggcctcg ccgcagatgc
     8461 cggcctaggc cggcgccggg tgcgcaggta gagtcccgcc gatgccggtc tccgtcgtca
     8521 cggacaccac cggatacctg ccgcgggagc acctcgaccg ctacgggatc acggcggttc
     8581 cgctctatgt cgtcttcgcc gaccgcacgg tcaaggagac cgagatcacc gactacccgg
     8641 cgttcttcga ggagctgcgc cgcgccgaca gcgtgccgaa gacctcccag ccctcggtcg
     8701 gcgacttcgt cgcggcgttc gagccgctgc tcgcgcgcgg cgacgagatc gcttcgattc
     8761 acatctcggg cggcatctcg ggcaccgtcg cgttccagct gctcggccgc ctggcgcgct
     8821 gactcgacgg tgcccgagat gccgcccgag atgagaatcg aagcgatctc gtcgacgcgc
     8881 gcgag
//
LOCUS       DATABV010000192         5921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_945840, whole genome shotgun sequence.
ACCESSION   DATABV010000192 DATABV010000000
VERSION     DATABV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945840"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VHF88_08710"
     CDS             complement(<1..1202)
                     /locus_tag="VHF88_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2161886.1"
                     /translation="MTLARSRCALAAAALALAATGCSDDAEPRDEAGAGGSLVVAEAT
                     APGSLDPALVATPVARRAAWLAYTPPLTYRRVEGEDGTELVPALSEDLPETSDDGRSY
                     SFRLRRGVRYSNGMVLRARDFERAVARSLRLSPRGRALWGNVVGARAYARGDGESTDV
                     RGIAVDDRSGRVRIELERPDRLFPYALASLAAAPVPRGTPLRDLSADPPPGIGPYRVA
                     QVRRGGDVVLERRRNWRLPGIPAGKPREIVTRTIRDRAARVDAVLDGRADLVEGESPL
                     RMLPDIRSGTEGRYAEYPTLRTLYVAIDASRDPFRDGDVRRALSYSLDARVLAQLHDG
                     FMQPACNMLPPEVAGHRPLDPCPFGARDGDADLVEASRLVEHAGARGAVVRVAEGHDV
                     RGRRLARYL"
     gene            complement(1199..1960)
                     /locus_tag="VHF88_08715"
     CDS             complement(1199..1960)
                     /locus_tag="VHF88_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX2161887.1"
                     /translation="MNDYGNGARAARQREDRELLRRYHVDGDRSAREQLIARHLPLVR
                     SLARRYAGRGESLEDIEQVGAIGLIKAIDRFDLEREVSLATYATPNVVGEIKRHFRDK
                     GWAIRVPRSLQELNARMSGAIDDLTGRLGHSPSVAEIAESLGTSKEEVLEALEVGSAY
                     TALSLDAGPAGSDEDSDPMETIGGEDAEFDRSEDRATLGPVLDRLPEREREILRMRFE
                     EGLPQTQIAERVGLSQMHVSRLIRRSLATMREELE"
     gene            complement(1957..2367)
                     /locus_tag="VHF88_08720"
     CDS             complement(1957..2367)
                     /locus_tag="VHF88_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161888.1"
                     /translation="MAIDPRTTVERVEIDAPLDAPFHGVVRLIVSGIAEQANFGFEAM
                     DDLQLAIERLLAETAGEGRVSISFDIGDGSIRTRIGPLRERRLTAALQEPDGDAGTLS
                     LSRVLSAVVDSFGVEPAVDGQIVVRLEKLGTPRP"
     gene            complement(2357..3832)
                     /gene="mnmA"
                     /locus_tag="VHF88_08725"
     CDS             complement(2357..3832)
                     /gene="mnmA"
                     /locus_tag="VHF88_08725"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX2161889.1"
                     /translation="MRSAELIEQHLRQPHGLGALGGAPHTGAAGGAACGDLVRISVRV
                     DGDRVAETGFEASGCAAVRAAGSAAAELVEGMPVLAAALVSPAVVAEELGGLAPSHMH
                     AAELTCDALHRALGSAARGGDVRIAASATRTLVAMSGGVDSAAAAQLALDAGDEVVAV
                     TLELWSDPAGDGEQSCCSPQAVRGARGLAQRMGIPHFTLDLRDRFRGDVVDDFLASYA
                     GGATPNPCVRCNGLVRFDAMVELADRLGAARLTTGHYARIEHDDEGPVVRAAVDPRKD
                     QSYALARVTTATLRRVEFPLGGYEKPAVRELARRAGLPVADKPESQDLCFLAGTGQQA
                     FVERHGTAAMHGAAGDIVDREGRVLGHHEGHHRFTVGQRRGIRVAAEEPLYVLAKDAR
                     RNRVVVGPRDQLATTTVRLGPGRLHRSSATVDAVRLRYQGAVLGCSVDRDLPAGEHHG
                     LTIELEHEVAMPAPGQVACLMRGEHVVGWALIEEPEVARGD"
     gene            complement(4127..>5921)
                     /gene="aspS"
                     /locus_tag="VHF88_08730"
     CDS             complement(4127..>5921)
                     /gene="aspS"
                     /locus_tag="VHF88_08730"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX2161890.1"
                     /translation="RRVSADTFDNRYRSAWCGLVRKARVGDELRVAGWVHRRRDHGGL
                     IFIDLRDREGLLQLVFHPDSAPDAHAAAHRLRSEDVISAAGRVVERSPETVNPELPTG
                     AVELEVSSFELLADAETPPFQIDEDDKVGEELRLRYRYLDLRREQMATAIRLRHELTA
                     AIRRYLDGRGFLDVETPILTRSTPEGARDHLVPSRNQPGSFFALPQSPQLFKQLLMVA
                     GFERYYQIARCFRDEDTRADRQPEFTQLDIELSFVEEEDVIQLVDGLIPELLRLGGID
                     VAAPIERLPYDEALLRYGSDRPDRRIPIEIADLGEVFAASEFKVFSGALASGGTIRGF
                     SASGDFPRRRFDELTEQAQALGAKGLVWGIVEDDGSWRSPVAKFLTEDEIGGLISATG
                     AKPGDAILAVADDPAVAARVLGELRLAVGVPEDRHDLVWIVDFPMFGWNADERRWDAL
                     HHPFTAPSGDLEDDPGSWRSRAYDLVWNGTEIGGGSIRINSPVVQQRVFDALGLAPDE
                     ARERFGFLLDALAYGAPPHGGIAFGIDRIVALLAGRDSIREVIAFPKTATGADPLTGA
                     PAPVDRRQLEDLGLKLVGGLAPPRPPAAPGR"
BASE COUNT          808 a         2166 c         2168 g          779 t
ORIGIN      
        1 aggtagcgcg cgaggcggcg gccgcggacg tcgtgtccct cggcgacgcg cacgacggcg
       61 ccacgggcgc cagcatgctc gacgagccga ctcgcctcga cgaggtcggc gtcgccgtcg
      121 cgggcgccga acggacaggg gtcgagcggg cggtgcccgg cgacctccgg cgggagcatg
      181 ttgcaggcgg gctgcatgaa cccgtcgtgg agctgcgcca ggacgcgggc gtcgagcgag
      241 tagctgagcg cgcgccgcac gtcaccgtcg cggaacgggt cgcggctggc gtcgatcgcc
      301 acatagagcg tgcgcagcgt cgggtactcc gcgtagcgac cctcggtgcc cgagcggatg
      361 tcgggcagca tccgcagcgg cgactcgccc tcgaccaggt cggcacgccc gtcgagcacg
      421 gcgtcgacgc gcgccgcgcg gtcgcggatc gtccgcgtga cgatctcgcg cggcttgcct
      481 gcgggaatgc ccggcagacg ccagttgcgt cggcgctcga gcacgacgtc cccgccgcgc
      541 cgtacctggg cgacgcggta gggaccgatg ccgggcggcg ggtcagccga caggtcgcgc
      601 aacggcgtcc cgcgcggaac cggggccgcg gcaagactcg ctagcgcgta cgggaagagg
      661 cggtccggac gctcgagctc gatcctgacc cggccgctgc gatcgtccac ggcgatgccg
      721 cggacgtcgg tcgactcccc gtcaccgcga gcgtaggcac gcgccccgac gacgttgccc
      781 caaagcgccc gtccccgcgg cgacaggcgc agcgagcgcg cgacggcgcg ctcgaagtcg
      841 cgggcgcgca acaccatccc gttcgagtag cgcacgcccc gccgcagccg gaagctgtag
      901 ctgcgcccgt cgtcggaggt ctcgggcagg tcctcgctga gcgccggcac gagctcggtg
      961 ccgtcctcgc cctcgacgcg gcggtaggtc aacggcggcg tgtaggcgag ccaggccgcg
     1021 cgacgcgcga ccggggtcgc gaccagcgcc ggatcgagcg atcccggagc cgtcgcctcg
     1081 gcgacgacca gcgaacctcc cgcaccggcc tcgtcgcgcg gctcggcatc gtcgctgcag
     1141 ccggtcgccg ccagcgccag cgccgccgcg gccagcgcac accgcgatcg ggccaaggtc
     1201 attccagctc ttcgcgcatc gtcgcgagcg agcgacgaat caggcgcgag acgtgcatct
     1261 gcgacaagcc gacgcgctcg gcgatctgcg tctgcggcag gccctcctcg aagcgcatcc
     1321 gcaggatctc gcgctcgcgc tcgggcagac gatcgagcac gggtccgagc gtcgcgcggt
     1381 cctcgctgcg atcgaactcg gcatcctcgc cgccgatcgt ctccatcgga tcgctgtctt
     1441 cgtcgctgcc cgccggaccg gcgtcgagcg acagcgcggt gtaggcggag ccgacctcga
     1501 gcgcctcgag gacctcctcc ttgctcgtcc cgagcgactc ggcaatctcg gccacgctcg
     1561 gcgagtggcc gagccgcccg gtcaggtcgt cgatcgcgcc ggacatccgc gcgttgagct
     1621 cctggagcga gcgcggcacg cggatcgccc aacccttgtc gcggaagtgg cgcttgatct
     1681 ctccgacgac gttcggggtc gcgtaggtcg cgagcgacac ctcgcgctcg aggtcgaagc
     1741 ggtcgatcgc cttgatcagt ccgatcgccc caacctgttc gatgtcctcg agcgactcgc
     1801 cgcggccggc gtagcggcgg gccagcgagc ggacgagcgg cagatgacgg gcgatcagct
     1861 gctcgcgcgc cgaacggtcg ccgtcaacgt gatagcggcg cagcagctcg cggtcctcgc
     1921 gctggcgcgc ggctcgcgcg ccgttcccgt agtcgttcac gggcggggcg ttcccagctt
     1981 ctccaacctc accacgatct ggccgtcgac ggccggctcg acgccgaacg agtcgacgac
     2041 ggcggagagc acgcgcgaca gtgacagcgt tccggcatcg ccgtcgggct cctgcagcgc
     2101 ggctgtgagc cggcgctcgc gcagcgggcc gatgcgcgtg cggatgctgc cgtcgccgat
     2161 gtcgaaggag atgctgacgc gaccttcacc ggcggtctcg gcgagcagcc gctcgatcgc
     2221 cagctggagg tcgtccatcg cctcgaagcc gaagttcgcc tgctcggcaa tcccgctgac
     2281 gatcagccgc acgacgccgt ggaagggagc gtcgagcggc gcatcgatct cgacgcgctc
     2341 gaccgtcgtg cgcggatcaa tcgccacgag ccacctccgg ctcctcgatc agcgcccagc
     2401 cgaccacgtg ctcaccgcgc atcaggcacg cgacctgccc tggtgccggc atcgctacct
     2461 cgtgctcgag ctcgatcgtc aggccgtggt gctcgcccgc cggcaggtcg cggtcgaccg
     2521 agcagccgag cacggcgccc tggtagcgca gacggaccgc gtcgaccgtg gccgacgagc
     2581 ggtgcagccg cccgggcccg agccgcacgg tcgtcgtcgc gagctgatcg cgcggcccaa
     2641 ccacgacgcg gttgcgacgc gcgtccttgg ccagcacgta caacggctcc tcggcggcga
     2701 cgcggattcc gcgccgctgg ccgacggtga agcggtgatg cccctcgtga tggcccagca
     2761 cgcgtccctc cctgtcgacg atgtcgcccg cggcgccgtg catcgcggcg gtcccgtgac
     2821 gctccacgaa ggcctgctgg cccgttccgg cgaggaagca gagatcctgg ctctccggct
     2881 tgtcggcgac cggcagcccg gcgcggcgcg ccagctcgcg aacggccggc ttctcgtagc
     2941 cgccgagcgg gaactcaacg cgtcgcagcg tggcggtggt cacgcgtgcc agcgcgtagc
     3001 tctggtcctt gcgcgggtcg acggccgcgc ggaccacggg cccctcgtcg tcgtgctcga
     3061 tccgggcgta gtgcccggtc gtcagccgcg cggcgccgag cctgtcagcc agctcgacca
     3121 tcgcgtcgaa gcggaccagg ccgttgcaac ggacgcaggg gttcggcgtc gcgccaccgg
     3181 cgtagctggc gaggaagtcg tccaccacgt cgccgcggaa gcggtcgcgc aggtcgagcg
     3241 tgaagtgcgg gatccccatc cgctgggcca gcccgcgggc gccgcgcacg gcctgcggcg
     3301 agcagcagct ctgctcgccg tcgccggccg ggtcagacca cagctcgagc gtgaccgcga
     3361 ccacctcgtc tccggcgtcg agcgcgagct gcgccgccgc ggcgctgtcg acgccgccgc
     3421 tcatcgcgac cagcgtgcgc gtcgccgacg cggcgatccg cacgtcgccc ccgcgcgcgg
     3481 ccgatccgag cgcccggtgc agggcgtcgc aggtcagctc cgcggcatgc atgtggctcg
     3541 gggcgaggcc gccgagttcc tcggcgacga ccgccggcga gaccagcgcg gcggcgagca
     3601 ccggcatgcc ctcgaccagc tcggctgccg cgctgccggc ggcacgcacc gcggcgcagc
     3661 ccgacgcctc gaagcccgtc tcggcgacgc ggtcgccgtc gacgcgcacc gagatccgca
     3721 cgagatcccc gcaggcggcg ccgccggcgg cgccggtgtg cggcgccccg cccagtgccc
     3781 ccaagccgtg gggctgcctc agatgctgct cgatcagctc cgcgctgcgc attcgcgtcc
     3841 actctacgga atacgagctg gcggaaaagg cattaacccc agcttgccgc tgccggtgac
     3901 cgggttgggc ctgattctgg caccgtgggc gtcctccgga ccgtgacttg cggcagagcg
     3961 cctgtcacgc cccgagccgg acacccatcc cgttggtgtt ggctcaacat gtgggccggc
     4021 tggcgcacaa gctagggctg tgcccaccgt agcaccgcca gaacgccgaa accaggccta
     4081 ccggccaggt gccgcggggg gcccgggggg cgcgagcccc aaccccctac cggccaggtg
     4141 ccgcgggggg cctggggggc gcgagccccc cgaccaactt caggccgaga tcctcgagct
     4201 ggcggcgatc taccggcgcc ggtgcaccag tcagggggtc ggcgccggtg gcggtcttcg
     4261 ggaaggcgat cacctcacga atcgagtcgc ggccggcgag cagggcgacg atgcggtcga
     4321 tcccgaatgc gatcccgccg tgcggcgggg cgccgtaggc gagcgcgtcg aggaggaagc
     4381 cgaagcgctc gcgcgcctcg tcgggggcga ggccgagcgc gtcgaagacg cgctgctgga
     4441 ccaccggcga gttgatgcgg atcgagccgc cgccgatctc ggtcccgttc cagaccaggt
     4501 cgtaggcgcg gctgcgccag ctcccggggt cgtcctcgag gtcgccgctc ggcgcggtga
     4561 acgggtggtg gagcgcgtcc cagcggcgct cgtcggcgtt ccagccgaac atcgggaagt
     4621 cgacgatcca gacgaggtcg tggcggtcct cgggcacgcc gacggcgagc cgcagctcgc
     4681 cgagcacacg ggcggcgacc gccggatcgt cggcgacggc gaggattgca tcgccgggct
     4741 tcgccccggt cgcggagatc aggccgccga tctcgtcttc ggtgaggaac ttggcgaccg
     4801 gcgagcgcca gctgccgtcg tcctcgacga tcccccagac gaggcccttc gccccgagcg
     4861 cctgcgcctg ctcggtcagc tcgtcgaagc gccggcgcgg gaagtcgccg gaggcgctga
     4921 agccgcggat cgtcccgccg gatgccagcg cgccgctgaa gaccttgaac tccgaggctg
     4981 cgaagacctc gccgaggtca gcgatctcga tcgggatgcg tcgatcgggc cggtcgctgc
     5041 cgtagcgcag cagcgcctcg tcgtatggca gccgctcgat cggcgccgcg acgtcgatcc
     5101 cgccgagccg cagcagctcg gggatcagcc cgtcgacgag ctggatcacg tcctcctcct
     5161 cgacgaacga gagctcgatg tcgagctggg tgaactccgg ctgtcgatcg gcgcgcgtgt
     5221 cctcgtcgcg gaagcagcgg gcgatctggt agtagcgctc gaagccggcg accatcagca
     5281 gctgcttgaa gagctgcggc gactgcggca gcgcgaagaa cgagcccggc tggttgcggc
     5341 tcggcacgag gtggtcgcgt gcgccctcgg gcgtcgaacg agtcaggatc ggcgtctcga
     5401 cgtccaggaa gccgcggccg tcgaggtagc ggcggatcgc cgcggtgagc tcgtggcgga
     5461 gccggatcgc cgtcgccatc tgctcgcggc gcaggtcgag atagcggtag cgcaggcgca
     5521 gctcctcgcc gaccttgtcg tcctcgtcga tctggaaggg cggcgtctcg gcgtcggcga
     5581 gcagctcgaa cgagctcacc tcgagctcga ccgctccggt cggaagctcc gggttgacgg
     5641 tctcggggct gcgctcgacg acccggcccg ctgccgatat gacgtcctcg gagcgcaggc
     5701 ggtgcgccgc ggcgtgcgca tccggggcgc tgtcggggtg gaagacgagc tgcagcaggc
     5761 cctcgcggtc gcgcaggtcg atgaagatca ggccgccgtg gtcgcggcgg cggtggaccc
     5821 agcccgccac ccgcagctcg tcgccgaccc gcgccttgcg gaccaggccg caccaggcgc
     5881 tgcggtagcg gttgtcgaac gtgtcggcgc tcacgcgtcg a
//
LOCUS       DATABV010000193         7657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1026533, whole genome shotgun sequence.
ACCESSION   DATABV010000193 DATABV010000000
VERSION     DATABV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026533"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..585)
                     /gene="rpsP"
                     /locus_tag="VHF88_08735"
                     /pseudo
     CDS             complement(361..585)
                     /gene="rpsP"
                     /locus_tag="VHF88_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(654..1982)
                     /gene="ffh"
                     /locus_tag="VHF88_08740"
     CDS             complement(654..1982)
                     /gene="ffh"
                     /locus_tag="VHF88_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX2161891.1"
                     /translation="MFETLGERLQGALGDVRSRGALTEDDVAKAMRQVRLALLEADVN
                     FKVVKQFTSAVKERATGSDVLDSLNPGQQVVKVVADELTELMGGSGRDLVFSKSGPTV
                     ILMAGLQGSGKTTACGKLARHLKEEKGLDVALAACDLQRPAAVDQLVKVGSQVGATVY
                     EQGTGRDPVDVAEWALGQARADKRDVLIVDTAGRLHVDSELMDQLSQVKQRTKPHDVL
                     LVVDAMTGQDAVNVAEAFAEATAFDGVVLSKLDGDARGGAALSVKAITDKPILYASTG
                     EKLDAFERFHPDRMAQRILGMGDVMTLIEKAEARIDERQAQELERKIRKNQFTLDDFL
                     EQMQQVRSMGPLQNILKMLPGVGAQLNGLTVDDKELDRLQAIITSMTPAERANPKIID
                     GSRRRRIAAGSGTNVQAVSNLVKQFGQMQKLMRQMARGKMPSLAQIASGR"
     gene            complement(1987..2886)
                     /locus_tag="VHF88_08745"
     CDS             complement(1987..2886)
                     /locus_tag="VHF88_08745"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX2161892.1"
                     /translation="MLVGAKAREAIVESGAARGVEAISDAGHRVTVRSRAVVVACGAV
                     QTPALLRRSGLRNRNIGQHLHLHPVTVAWGLFDDELRPWEGAMQALYSDEHAELDDGY
                     GVKYETAAMHPHVVNGFWPWRGGRAHHELMRGLPHATGVGALLRDRDAGEVRVGRDGE
                     PVVRYRLSSFDRDNLRSGLDGAAAILEAAGARRIFSSHSDWVSYDPGGHGDRRRFMRD
                     ADRCGWGPGQISLASFHIMGSARMGGTPSTSACTPAAETWDVRDMVVCDASTFPSASG
                     VNPMVSIEAIAHMNASALAARLA"
     gene            complement(2796..3935)
                     /locus_tag="VHF88_08750"
     CDS             complement(2796..3935)
                     /locus_tag="VHF88_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2161893.1"
                     /translation="MELSDGQRGALAAICDTVCPGGDGLPSASELGVPEALREAVGRN
                     PRAAERRQLAQLLSAWDSQPLTALGGGGWRRFSELSQEGRERVLLSWCDSRLPQRRAA
                     FHALCRGTRLFYWMLPAPDGGRSPAWDDIGYPGPLGPNPEAPPKAIAPLEIDRDTELE
                     CDVVVVGSGAGGGTAAAVLAGAGLDVVVLEAGPYSDDADFDGSERTAIRTLYAGAPTA
                     TVDQSLSLLAGACLGGGTVINYSTSFRTPDAIRADWAGHGVHAFAERDFDASLDAVCE
                     RLGVNHEHAAPSSRDRALRDGCMALGWHVDAMPRNVRGCDQGEVCGYCPFGCRLGAKQ
                     STLKPGSPMRTRPGRESSSVPRRARRSSRAGRRAASRRSATPATA"
     gene            complement(3940..4887)
                     /locus_tag="VHF88_08755"
     CDS             complement(3940..4887)
                     /locus_tag="VHF88_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2161894.1"
                     /translation="MATLIVLGVVALVVLFTIAQGVRIVPQARAGVVERLGRYSRTLD
                     AGLALIVPYVDRVKPLIDLREQVVSFPPQPVITEDNLVISIDTVIYFQVTDPKSATYE
                     IADYIGAIEQLTVTTLRNVIGGMTLEDTLTSRDKVSGALRVVLDEATGRWGIRVNRVE
                     LKAVDPPASIQQAMEKQMRAERDRRAAILTAEGVKQSQILTAEGEKQSAILRAEGDRQ
                     AQILEAEGEAQAIATIFEAVHRGDADEKVLAYQYLQKLPDIADGEASKLWIIPSELTN
                     ALGRLGSMLGSEAGIGPGDGATPAAAQKPPAPPEPPRPV"
     gene            complement(4897..5331)
                     /locus_tag="VHF88_08760"
     CDS             complement(4897..5331)
                     /locus_tag="VHF88_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX2161895.1"
                     /translation="MSAWVIWIVAAGLLAIGEIVSLSFFLGPIAVAAVAAAVAALVGA
                     GTALQLVVFILASIASLIVLRPIARRHLHTPAKIRTGTAALIGARAIVLERVDRNGGT
                     VKLSGEVWSARPYDEDEVLEPGARVEVMQIEGATALVSDGIG"
     gene            5502..6512
                     /gene="ftsY"
                     /locus_tag="VHF88_08765"
     CDS             5502..6512
                     /gene="ftsY"
                     /locus_tag="VHF88_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX2161896.1"
                     /translation="MAREWNELFVVDGVPIEGSRADAAIEAQDEEQRPKRRFFKRLRE
                     NMSKTRQALGAEIQATMFERLDDETFERLEEALIYADVGAPTTASIVERLEGEAASGD
                     LESGEDLTRRLKELLAETARVEGDTIDLSAHPTVILMVGVNGTGKTTTIGKIAWHLQK
                     ELGRSVLLAAGDTFRAAAAEQLEVWAERAGCDIVRGEEGSDPGSVMFDAIEAAAARGH
                     DVVLCDTAGRLHTQQHLMDELAKVRKVIASKIPDAPHHTLLTIDATTGQNGLRQALLF
                     RETVDLDGIVLTKLDGTAKGGIALAIAQELNVPVKMIGIGEGIEDLRPFDPDDFAAAI
                     LD"
     gene            complement(6513..>7657)
                     /locus_tag="VHF88_08770"
     CDS             complement(6513..>7657)
                     /locus_tag="VHF88_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2161897.1"
                     /translation="LATAADKAAAALDEAREAVAARVALIEAEHAAGAAAGERTAAEL
                     RSCAHQESELQRELRDASERVTVAEVTAQQIRDQAADATGELDQVAERLGLPAEPADE
                     PLDEERRAELATRIDRLTRRREQLGPVNPLAAQEYEEAIAHVEELEGQRKDLENALSE
                     LQGLIRDTDRRIRESFEETYEAAAKNFEEVVAHLFPGGRGRLRLVQPDQGPRPVLGGQ
                     EADGAGEDAATEDAEAEAGPEESAAKPAASEPGVEIEVTPAGKSTKRLSLMSGGEKSL
                     VALAFMFAVFLARPCPFYVLDEVEAALDDLNVDRFLQLVRRYSERAQFIVVTHQKRTM
                     DAADRIYGVSMGGDGVSRVVSRQLSGTAGENLFDQAKPVESSAGAA"
BASE COUNT         1057 a         2712 c         2734 g         1154 t
ORIGIN      
        1 cctcggcgaa cgagtcgacc gagatcgcct cggggtcgtc gacgagagcg cgagccaggt
       61 aggcgagcag ctcctccacg cggacggcta cgggttctcg tcggcgtccg cgggcgcctc
      121 ggcctcgtcg gccgcgacgt caccctcggg cacgtcgctc gagggctcct cgccggggcc
      181 gccgtcgccc gcctcgttct cggcaggcgg ctgctccgct gctgcgtcgt cgtcggcggc
      241 atcgggcaca gcggcctccg cattgccctg cgactgctcg gccgcgggcg cctcgcgctt
      301 ggcggccggc accggcgccg gtgcgaccgc cgccggcacc tcgtggccgg tgttctcggc
      361 gcgcatcagc ttcttgaccg tgttgctcgg ctgggcgccg cgatcgatcc agtgctggag
      421 acgctcgcgg tcgatctcga tccgcgacgg ctgcgtctgc gggttgtagt gcccgatcgt
      481 ctcgatcgtg cgaccgtcgc gcggcgagcg gccgtcagcg accactactc gccagatcgg
      541 gttcttcttt gagccgacac gggtcagcct gacttttacg gccaaggtct cttctctgat
      601 cgggttgtaa gagcgctttg cccgtagagc ggtctacagg cggctcggga acgttagcgg
      661 cccgaggcga tctgcgccag cgagggcatc ttcccgcgcg ccatctgacg catcagcttc
      721 tgcatctgtc cgaactgctt gaccaggttt gagaccgcct ggacgttggt tcccgagccc
      781 gccgcgatcc tgcgccggcg cgagccgtcg atgatcttcg ggttggcgcg ctcggccggc
      841 gtcatcgagg tgatgatcgc ctggaggcga tcgagctcct tgtcgtcgac ggtcaggccg
      901 ttgagctgcg ccccgacgcc cggcagcatc ttgaggatgt tctgcagtgg ccccatgctg
      961 cgcacctgct gcatctgctc gaggaagtca tcgagcgtga actggttctt gcggatcttg
     1021 cgctcgagct cctgggcctg gcgctcgtcg atgcgcgcct cggccttctc gatcagcgtc
     1081 atcacgtcgc ccatgccgag gatccgctgg gccatccgat cggggtggaa gcgctcgaag
     1141 gcgtcgagct tctcgccggt cgaggcgtag aggatcggct tgtcggtgat cgccttgacc
     1201 gacagcgccg cgccaccgcg ggcgtcgccg tcgagcttgc tcagcacgac gccgtcgaac
     1261 gcggtcgcct cggcgaatgc ctcggcgacg ttgacggcgt cctggccggt catcgcatcg
     1321 acgaccagca gcacgtcgtg cggcttggtc cgctgcttga cctggctgag ctggtccatc
     1381 agctcgctgt cgacgtgcag ccggccggcg gtgtcgacga tcagcacgtc gcgcttgtcg
     1441 gcacgcgcct ggccgagcgc ccactccgcg acgtcgacgg ggtcgcgccc ggtgccctgc
     1501 tcgtagacgg tcgcgccaac ctgcgagccg accttgacga gctggtcgac cgccgcgggg
     1561 cgctgcaggt cgcaggccgc gagcgcgacg tcgaggccct tctcctcctt caggtgccgg
     1621 gcgagctttc cgcaggcggt cgtcttgccc gaaccctgca gaccagccat caggatcacc
     1681 gtcgggccgc tcttggagaa gaccaggtcg cggccggagc cgcccatcag ctcggtcagc
     1741 tcgtcggcca cgaccttgac gacctgctga cccgggttga gcgagtcgag cacgtcggag
     1801 ccggtcgccc gctccttgac ggcgctcgtg aactgcttga cgaccttgaa gttgacgtcg
     1861 gcctcgagca gcgcgagccg cacctggcgc atcgccttgg cgacgtcgtc ctcggtcagc
     1921 gcgccgcgcg agcggacgtc gccgagggcg ccctgcagcc gctcaccgag agtctcgaac
     1981 acgctctcag gctagccggg cggcgagcgc ggaggcgttc atatgggcga tcgcctcgat
     2041 cgagaccatc gggttgacgc cggaggcact cgggaacgtc gacgcgtcgc agacgaccat
     2101 gtcgcggacg tcccaggtct cagcggccgg cgtgcaggcc gaggtcgacg gcgtgccgcc
     2161 catccgggcg ctgcccatga tgtggaagga cgccagcgag atctgtcccg gcccccagcc
     2221 gcagcggtcg gcgtcgcgca tgaagcgcct gcggtcgccg tggccgccgg ggtcgtagga
     2281 gacccagtcg gagtgcgagc tgaagatgcg ccgcgcgccg gcggcctcga ggatcgcggc
     2341 cgccccgtcg aggccgctgc gcaggttgtc ccggtcgaag ctcgacagcc ggtagcgaac
     2401 gacgggctcg ccgtcgcggc cgacgcgcac ctcgccggcg tcgcggtcgc gcagcagcgc
     2461 gccgaccccg gtcgcgtggg gcagcccgcg catcagctcg tggtgggcgc gtccgccacg
     2521 ccagggccag aagccgttga cgacgtgcgg gtgcatcgcc gccgtctcgt acttgacgcc
     2581 gtagccgtcg tcgagctcgg cgtgctcgtc ggagtagagc gcctgcatcg caccctccca
     2641 gggccgcagc tcgtcgtcga acaggcccca tgcgacggtc accggatgga gatgcaggtg
     2701 ctggccgatg ttgcggttgc gcaggcccga gcgccgaagc agcgcggggg tctgcacggc
     2761 cccacaggcg accacgacgg cacgcgagcg gaccgtcacg cggtggccgg cgtcgctgat
     2821 cgcctcgacg ccgcgcgccg ccccgctctc gacgatcgcc tcgcgcgcct tggcaccgac
     2881 gaggattcgc gtcccggccg cgtgcgcatc ggcgagccag gcttgagcgt cgactgcttc
     2941 gccccgagcc ggcagccgaa cgggcagtag ccgcagacct cgccctggtc gcagccgcgg
     3001 acgttgcgcg gcatcgcgtc gacatgccag ccgagcgcca tgcagccgtc gcgcagcgct
     3061 cggtcgcgcg acgagggcgc cgcgtgctcg tgattgacgc cgagccgctc gcagaccgcg
     3121 tcgaggctcg cgtcgaaatc gcgctcggcg aaggcgtgga cgccgtggcc ggcccagtcg
     3181 gcacgaatcg cgtcgggcgt cctgaacgac gtcgagtagt tgatcaccgt gccgccgccg
     3241 aggcatgcgc cggcaagcag cgacaggctc tggtcgacgg tcgccgtcgg cgcaccggcg
     3301 tacaacgtcc ggatcgcggt gcgctcggag ccgtcgaagt cggcgtcgtc gctataaggg
     3361 cccgcctcga gcacgacgac gtcgagaccg gcgcccgcca gcaccgccgc agcggtccca
     3421 ccgcccgcgc ccgagccgac caccacgacg tcgcactcga gctctgtgtc gcggtcgatc
     3481 tccagcggcg cgatcgcctt cgggggcgcc tctgggttcg ggccgagcgg gccggggtag
     3541 ccgatgtcgt cccaggccgg gctgcgaccg ccgtccggcg ccggcagcat ccagtagaag
     3601 aggcgcgtgc cgcgacagag cgcgtggaag gcggcgcgcc gctggggcag ccgcgagtcg
     3661 caccaggaca gcagcacgcg ctcgcgaccc tcctgcgaca gctcggagaa ccgcctccag
     3721 ccaccgccgc ccagtgcggt cagcggctgg ctgtcccagg ccgagagcag ctgggcgagc
     3781 tggcgccgct ccgctgcacg cgggttgcgg ccgaccgcct cgcgcagcgc ctccggcaca
     3841 ccgagctcgc tggccgacgg cagtccgtcg ccgcctgggc agaccgtgtc gcagatcgcc
     3901 gcgagagcgc cgcgctgacc gtcgctgagc tccatccccc tacacgggtc gcggcggctc
     3961 cggcggggcc ggcggcttct gagcggccgc cggcgtcgcg ccgtcacccg gtccgatccc
     4021 ggcctcggaa cccagcatcg acccgagcct cccgagcgcg ttggtcagct cgctcgggat
     4081 gatccacagc ttgctcgcct cgccgtcggc gatgtcgggc agcttctgca ggtactggta
     4141 ggcgaggacc ttctcgtccg cgtcgccgcg gtggaccgcc tcgaagatgg tggcgatcgc
     4201 ctgcgcctcg ccctcggcct cgaggatctg cgcctggcgg tcgccctccg cacgcaggat
     4261 cgcgctctgc ttctcgccct ccgccgtcag gatctgcgac tgcttgacac cctcggcggt
     4321 caggatcgcg gcacggcggt cgcgctcggc gcgcatttgc ttctccatcg cctgctggat
     4381 cgacgcgggc gggtcgacgg ccttgagctc gacccggttc acgcgaatgc cccagcggcc
     4441 ggtcgcctcg tcgagcacca cccgcagcgc gcccgacacc ttgtcgcgcg acgtcagcgt
     4501 gtcctcgagc gtcatcccgc cgatcacgtt gcgcaaagtc gtcaccgtca gctgctcgat
     4561 cgcgccgatg tagtcggcga tctcataggt cgccgacttc gggtcggtca cctggaagta
     4621 gatgaccgtg tcgatgctga tcaccaggtt gtcctcggtg atcaccggct ggggcgggaa
     4681 cgacacgacc tgctcgcgca ggtcgatcag cggcttgacc cggtcgacgt aagggacgat
     4741 cagcgccagc ccggcatcga gcgtacggct gtagcgaccg agccgctcga ccaccccggc
     4801 ccgggcctgc ggcacgatcc gaacaccctg cgcgatggtg aacagcacca ccagcgccac
     4861 cactccaaga acgatcaaag tcgccacaac agcctcctag ccgatcccgt cggacacgag
     4921 cgccgtggcg ccctcgatct gcatcacctc gacccgagcc cccggctcga gcacctcgtc
     4981 ctcgtcatac ggtcgcgccg accacacctc gcccgacagc ttcaccgtcc cgccgttgcg
     5041 gtcgacgcgc tccaagacga tcgcgcgcgc tccgatcagc gccgccgtgc cggtgcgaat
     5101 cttggccggc gtgtgcaggt ggcgccgcgc gatcggtcgc aggacgatca gcgacgcgat
     5161 cgaggcgagg atgaagacga cgagctggag cgcggtgccg gcgccgacga gcgcggcgac
     5221 ggcggccgcc acggcggcga cggcgatcgg gccgaggaag aacgacaacg agacgatctc
     5281 gccgatggcg agcagtccgg ctgcgacgat ccagatgacc caggcggaca tggggttgat
     5341 cctatctggg cggctggtgg gctctggcta gggatcggcg ggggtggccg cgggtcctgt
     5401 ctccgatttt ggtttacgtg ctcgttcctg cgcgcgcaaa cgcaaaatcg tcgccacccg
     5461 cggaagcggc gctggtcttg cggcctatgc tctcggcgct gatggcgcga gagtggaatg
     5521 agctgttcgt cgtggacggg gtgccgattg aggggtcccg ggctgacgct gcgatagagg
     5581 cgcaagacga ggagcagagg ccgaagcggc ggttcttcaa gcggttgcgg gagaacatgt
     5641 cgaagacgcg gcaggcgttg ggcgcggaga ttcaggcgac gatgttcgag cggctggacg
     5701 acgagacgtt cgagcggctg gaggaggcgc tgatctacgc agacgtggga gcgccgacga
     5761 cggcgtcgat cgtggagcgg ctcgagggcg aggcggcgtc gggcgatctg gagagcggcg
     5821 aggatctgac gcggcggctg aaggagctgc tcgcggagac ggcgcgcgtc gagggcgaca
     5881 cgatcgatct gtcggcgcac ccgacggtga tcctgatggt cggcgtcaac ggaactggca
     5941 agaccacgac gatcggcaag atcgcctggc atctgcagaa ggagctcggc cgctcggtcc
     6001 tgctcgcggc cggcgacacc ttccgcgcgg ccgccgcgga gcagctcgag gtctgggccg
     6061 agcgagccgg ctgcgacatc gtgcgcggcg aggaggggtc ggaccccggc tcggtgatgt
     6121 tcgacgcgat cgaggccgcg gccgcgcgcg ggcacgacgt cgtcctctgc gacaccgccg
     6181 gccgcctcca cacccagcag cacctgatgg acgagctggc caaggtccgc aaggtgatcg
     6241 cgtcgaagat cccagacgca ccgcaccaca cgctgctgac catcgacgcc accaccggtc
     6301 agaacggcct acgccaagcc ctcctcttcc gcgagaccgt cgacctcgac ggcatcgtcc
     6361 tgaccaagct cgacggcaca gccaaaggcg gcatcgccct agccatcgcc caagagctca
     6421 acgtacccgt gaagatgatc ggcatcgggg agggcatcga ggacctccgc cccttcgatc
     6481 ccgacgactt cgccgccgca atcctcgact agctaggcag cgccggcgga agactcgacc
     6541 ggcttcgcct gatcgaacag gttctcgcca gcggtcccgc tgagctgccg cgacaccacc
     6601 cgcgacacgc catccccgcc catgctgacc ccgtagatcc ggtcggccgc gtccatcgtt
     6661 cgcttctggt gcgtcaccac gatgaactgc gcccgctccg agtaccgccg gacgagctgc
     6721 aggaagcggt cgacgttgag gtcgtcgagg gcggcctcga cctcgtcgag gacgtagaag
     6781 gggcacgggc gggcgaggaa gacggcgaac atgaaggcga gcgcgacgag cgacttctcg
     6841 ccgccagaca tcagcgagag gcgcttggtc gacttgccgg ctggggtcac ctcgatctcg
     6901 acgccgggct ctgaggcggc cggcttggcg gcggactcct ctggacccgc ctcggcctcg
     6961 gcatcctcgg tcgcggcgtc ctcgcccgcc ccgtcggcct cctggccgcc gagtaccggg
     7021 cgcgggccct ggtcgggctg gacgagacgc aggcgtccgc ggccgccggg gaagaggtgg
     7081 gccacgacct cctcgaagtt cttggcggcg gcctcgtagg tctcctcgaa gctctcgcgg
     7141 atgcgtcggt cggtgtcgcg gatcaggccc tggagctcgg agagggcgtt ctcgaggtcc
     7201 ttgcgctggc cctcgagctc ctcgacgtgg gcgatcgcct cctcgtactc ctgggcggcg
     7261 agcgggttga cggggccgag ctgctcgcgg cggcgggtga ggcggtcgat gcgggtggcg
     7321 agctcggcgc ggcgctcctc gtcgagcggc tcgtcggcgg gctcggcggg taggccgagg
     7381 cgctcggcga cctggtcgag ctcgccggtg gcgtcggcgg cctggtcgcg gatctgctgg
     7441 gcggtgacct cggcgacggt gacgcgctcg ctggcgtcgc gcagctcgcg ctgcagctcg
     7501 gactcctggt gggcgcagga gcgcagctcg gcggcggtgc gctcgccggc cgcggcgcct
     7561 gccgcgtgct cggcctcgat cagagcgacg cgcgcggcga ccgcctcgcg tgcttcgtcg
     7621 agcgccgcgg cggccttgtc ggccgctgtg gcgaggt
//
LOCUS       DATABV010000194         7397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_143148, whole genome shotgun sequence.
ACCESSION   DATABV010000194 DATABV010000000
VERSION     DATABV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143148"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VHF88_08775"
     CDS             complement(<1..509)
                     /locus_tag="VHF88_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2161898.1"
                     /translation="MAPAGELPSNPADLIGDTPMVRMLRIAPECGAELIAKLEVGNPA
                     GSVKDRIGVAMIDAAEAAGEIEPGETTIVEATSGNTGIALAFVCAARGYDLVLAMPEG
                     MSRERQSLLRLYGAEVQITESMGGMDEAVAAAREIAAARAPAFIPDQFSNPANPEIHR
                     RTTAEEIWR"
     gene            complement(553..951)
                     /locus_tag="VHF88_08780"
     CDS             complement(553..951)
                     /locus_tag="VHF88_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2161899.1"
                     /translation="MLTITTKSPYAVRALTELARRGEGEPVPIGEIAKAREIPVQFLE
                     GLFATLRRAGVLQSQRGVKGGYLFARPPADVTVLEVTELLDGELGSGPGATDQVWAEA
                     TGALKSALAATSIADLAEREAETNAPMYYI"
     gene            975..1361
                     /locus_tag="VHF88_08785"
     CDS             975..1361
                     /locus_tag="VHF88_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX2161900.1"
                     /translation="MRTQLRAGARFLVPLALMALIFAFSAMPSDGEDHGLAMLILRKL
                     THFGEYFALTGLWWWALREPVGGRRALLPALLIAIGYAITDEIHQTFVDGRVGTWRDV
                     LIDSAGALTAAWLIRRSLYPAATRSG"
     gene            complement(1332..2453)
                     /locus_tag="VHF88_08790"
     CDS             complement(1332..2453)
                     /locus_tag="VHF88_08790"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX2161901.1"
                     /translation="MATGDSAGVGKDRVQTLISKTPASAASGLGRSSEGHGRRLTAAF
                     EALEAFPALEESRTRVLRVVEEVPAETGKIVEAIESDVALVSTVLRLANGMETPRRGK
                     IADIREAIELLGWERVEQLALRAQTFDFFERSPGWDVMPERFRLHAVATQAAADKIAR
                     EIGFDRRDALLTAALVHDIGKLVLVQSYPAYPEQIHGDARTPGERVHRERLELGVDHA
                     VVGGVLARRWGLPDSLASSIERHHSEDADGQAAMIRLADMLAHYGQGEPIDRNELIAA
                     GTALDLEPDALRRLMYEFPYCPDSNAGQRRHVDPCPLSKRELDVLRKLAEGKVYKQIA
                     GELELSTSTVRTHLHNTYAKLGAADRAQAVLTATSRGWI"
     gene            complement(2542..3873)
                     /gene="gltX"
                     /locus_tag="VHF88_08795"
     CDS             complement(2542..3873)
                     /gene="gltX"
                     /locus_tag="VHF88_08795"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX2161902.1"
                     /translation="MSIRVRFAPSPTGALHIGGARTALYNWLLARGSGGDLVLRIEDT
                     DRERSTEENVAQILDALSWLELDFDEGPVFQSERSDRHRDVLAQLLDSGHAYRSNAAS
                     DEVKAWKAEHPGRGYRGSPESDGAIRLRVPDEGATVVHDVIRGDTSFDHVHIDDPVIA
                     RADGSVLYNFAVAIDDLDAGITHVVRGEDHLSNTPKQLLVLEALGAESPTYAHLPLLH
                     GPDGKKLSKRHGAASVQELRDAGYLPEAVRNYIALLGWSLDDSTTLMPTEQMVESFDL
                     ARVSKSPAVFDEQKLRWMNGRYLRDLEPAELARRLEQRLGRGGLEEAAALAQDKIQTL
                     EEFWPLAGFLVERQPYDEKAWAKVMRDGAAERLAAARAALATAEPFDVEGVETALRGV
                     VERLDVKPREVFQPVRLAIAGSTISPGIFESVAALGRDETLARVDDALARA"
     gene            3883..4746
                     /locus_tag="VHF88_08800"
     CDS             3883..4746
                     /locus_tag="VHF88_08800"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX2161903.1"
                     /translation="MLIGAHVSTAGGLVKAHGRGVEIGAEAIQVFNQSPRMWRPTRWK
                     DDDVAAFRELMADGPIGSVVIHAVYLINCASKDPEIRAKSRTSLIHSLRMGAAIGADG
                     VVLHPGSTVGEPHGEAIERVGELLREALAESESCPLLLEDTAGAGNTLGRSFEELAQL
                     IELAGGDDRLGLCLDSCHLLASGFDVRTPAGIEKVMDDCVAIVGEQRLRCVHVNDSQT
                     PLGSNRDRHAPLGDGELGAEGCGAFLSEPRFEGLPAIFEGPGVEGKAPSAVDIERAHE
                     LRSAGLAARGL"
     gene            4827..5138
                     /locus_tag="VHF88_08805"
     CDS             4827..5138
                     /locus_tag="VHF88_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161904.1"
                     /translation="MGLAANPDFQKWLDGYAARHEQLHGDGPTSPPGVCDGCGDPLAD
                     PPSSAPFCLVCLAEQYGRDRAQAAARIATALRMALAADDGTPEGDIREAVEDVLQDLA
                     S"
     gene            complement(5135..5518)
                     /locus_tag="VHF88_08810"
     CDS             complement(5135..5518)
                     /locus_tag="VHF88_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161905.1"
                     /translation="MEPTALPNLGDRHECRECCAFCDRVVHPAGCIESGCELLYLYDD
                     EETGARYMGCLNKVFRAEIDVAAYDAAQRTRHGYGGVKMTGAPLERCRSSVERAYDGH
                     GDAFECVNRGFLEKPAADFDLRDRL"
     gene            complement(5544..>7397)
                     /gene="dnaE"
                     /locus_tag="VHF88_08815"
     CDS             complement(5544..>7397)
                     /gene="dnaE"
                     /locus_tag="VHF88_08815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2161906.1"
                     /translation="YSMKPIEEIGLLKMDFLGLRNLDVIEDCLRIIEESGREKPDMAS
                     LPLDDPKTYEMMARGDSVGVFQFESEGMRDALRKVRPTEFEDLVALNALYRPGAMRHI
                     DTYARNKRNPEAVHYIDERLRPITEATYSVILYQEQSMQIAKAVAGFSGAEADDLRKA
                     IGKKNREAMAALKARFFEGARASGTQERVIEELWAVNEAAADYSFNRSHAACYGLISY
                     RTAWLRANYPAEYMAALISSVMSTKDKVPFFVSCCEEMGIEVLPPDVNESGHNFEVRE
                     GNIRFGLDAVKNVGSAAVDAIVAAREDGGPFASLWDFCRRVDCRSVNRKATEALIKCG
                     AFDSTGATRSGMLAVLEQAQGAGQKQQQDAALGQGSIFELEEPAPGGGAAGQPDLPVP
                     ALPDERPELNAWEKETLGLFLSSHPLKEVRAALRRKVDCSIADLPERKDQEWVTVGGI
                     VTECKKIRTRKGDPMLFATLDDLEGQVEMLVFAKVYADNADKVEVDSVITVRGRVDKK
                     EAGEVKLVAQEVAAFDPSPEEVELAAAEAAAEPQVTRLAKLVTLNVPGDVPESLLDEL
                     KQVVEIFPGDHQVLLRVGQRSLKLGDDFRIKAEDSACLAELGALPGVSMAA"
BASE COUNT         1004 a         2579 c         2667 g         1147 t
ORIGIN      
        1 tcgcgccaga tctcctcggc ggtcgtgcgg cggtggatct ccgggttggc gggattcgag
       61 aactggtcgg ggatgaacgc cggtgcgcgc gcggcggcga tctcgcgcgc ggcggcgacc
      121 gcctcgtcca tcccgcccat cgactcggtg atctggacct cggcgccgta gagccgcagc
      181 aggctctggc gctcgcggct catcccctcg ggcattgcca gcacgaggtc gtatccgcgc
      241 gccgcgcaga cgaaggccag cgcgatcccg gtgttgcccg acgtcgcctc gacgatcgtc
      301 gtctcccccg gctcgatctc gcccgccgcc tcggccgcgt cgatcatcgc cacgccgatc
      361 cggtccttga cgctgccggc cggattgccg acctcgagct tcgcgatcag ctccgcgccg
      421 cactccgggg cgatccgcag catgcgcacc attggcgtgt cgccgatcag gtcggcgggg
      481 ttggagggga gctcgcccgc tggggccatc aggcgagcct accgggcgcc gcagcgccca
      541 cagcggatcg cgctagatgt agtacatcgg cgcgttcgtc tcggcctcgc gctcggcgag
      601 gtcggcgatc gacgtcgcgg cgagggcgga cttcagcgca ccggtggcct cggcccagac
      661 ctggtcggtc gcaccggggc cggagccgag ctcgccgtcg agcagctccg tgacctcgag
      721 caccgtcacg tcggccggcg gacgggcgaa caggtagccg cccttgaccc cgcgctggct
      781 ctgcaggacc cccgcgcggc ggagcgtcgc gaacagaccc tcgaggaact ggactgggat
      841 ctcgcgcgcc ttggcgatct cgccgatcgg gaccggctcg ccctcgccgc ggcgcgccag
      901 ctcggtcagc gcacggaccg cgtagggcga cttggtcgtg atcgtcagca ccgggccgag
      961 tctaggttat ggccatgcgg acccaactcc gtgccggcgc ccgtttcctc gtcccgctgg
     1021 cgctgatggc gctgatcttc gccttctcgg cgatgccgag tgacggcgag gatcacggcc
     1081 tggcgatgct gatcctgcgc aagctgaccc atttcggcga gtacttcgcg ctgaccgggc
     1141 tctggtggtg ggcgctgcgc gagccggtcg gcggtaggcg cgcgctgctt cccgcgcttc
     1201 tgattgcgat cggctacgcg atcaccgacg agatccacca gaccttcgtc gacggtcgtg
     1261 tcggcacctg gcgtgacgtc ctgatcgact ccgccggcgc gctgacggcg gcctggctga
     1321 tccgccgttc cctatatcca gccgcgactc gtagcggtta ggaccgcctg agcgcggtcg
     1381 gccgcgccga gcttggcgta ggtgttgtgc aggtgggtgc ggacggtgct cgtcgacagc
     1441 tcgagctcgc cggcgatctg cttgtagacc ttgccctcgg cgagcttgcg cagcacgtcg
     1501 agctcccgct tgctgagcgg gcagggatcg acgtggcggc gctgcccggc gttggagtcg
     1561 gggcagtagg gaaactcgta catcagacga cggagggcgt cgggctcgag gtcgagcgcc
     1621 gtgccggcgg cgatcagctc gttgcggtcg atcggctcgc cctgcccgta gtgggcgagc
     1681 atgtcggcga ggcggatcat cgccgcctgg ccgtcggcgt cctcggagtg gtggcgctcg
     1741 atcgacgacg cgaggctgtc cggcagcccc cagcgccggg cgagcacgcc gccgacgacg
     1801 gcgtggtcga cgccgagctc gagccgctcg cggtgcacgc gctcacccgg cgtgcgcgcg
     1861 tcgccgtgga tctgctccgg ataggcggga taggactgga cgagcacgag cttgccgatg
     1921 tcgtggacga gcgcggcggt cagcagcgcg tcgcgccggt cgaagccgat ctcgcgggcg
     1981 atcttgtcgg ccgccgcctg cgtcgcgacg gcgtgcaggc ggaagcgctc gggcatcacg
     2041 tcccagcccg ggctgcgctc gaagaagtcg aaggtctgcg cgcgcagggc gagctgctcc
     2101 acccgctccc agccgagcag ctcgatcgcc tcgcggatgt cggcgatctt gccgcgccgc
     2161 ggcgtctcca tgccgttggc gagtcgcagg acggtcgaca cgagcgcgac gtcggactcg
     2221 atcgcctcga cgatcttgcc ggtctcggcc gggacctcct cgacgacgcg caggacgcgg
     2281 gtgcgcgact cctcgagcgc cgggaaggcc tcgagcgcct cgaacgccgc cgtcaggcgg
     2341 cggccgtggc cctcgctcga ccggccgagg ccgctggccg ccgaggcggg ggttttggag
     2401 atgagcgtct gcacgcgatc cttgccgacc ccagctgagt caccggtggc cattcacggc
     2461 acctatcggc accctgcgag aaggcttgag ccgtgccacg ggcgaagcgc ccggctctgc
     2521 cgggcgcgca cgcggatctc ctcaggcgcg ggcgagcgcg tcgtcgacgc gggcgagcgt
     2581 ctcgtcgcgt ccgagcgcgg cgaccgactc gaagatcccc ggtgagatcg tgctgccggc
     2641 gatcgccagc cgcaccggct ggaagacctc gcgcggcttg acgtcgagac gctcgacgac
     2701 gccgcgcagc gcagtctcga cgccctcgac gtcgaaaggc tcggccgtcg cgagcgcggc
     2761 gcgcgccgcg gcgaggcgct cggcggcgcc gtcgcgcatc accttcgccc acgccttctc
     2821 gtcgtagggc tggcgctcga cgaggaagcc cgcgagcggc cagaactcct cgagcgtctg
     2881 gatcttgtcc tgggccagcg cggcggcctc ctccaacccg ccgcgaccga gccgctgctc
     2941 gagccgtcgc gccagctcgg caggctcgag atcgcgcagg tagcggccgt tcatccagcg
     3001 cagcttctgc tcgtcgaaca ccgccggcga cttggagacg cgcgcgaggt cgaagctctc
     3061 gaccatctgc tcggtcggca tcagcgtcgt ggagtcgtcg agcgaccagc cgagcagcgc
     3121 gatgtagttg cgcaccgcct ccggcaggta gccggcgtcg cgcagctcct ggaccgaggc
     3181 cgccccgtgg cgcttggaga gcttcttgcc gtcggggccg tgcagcagcg gcaggtgggc
     3241 gtaggtcggc gactcggcgc cgagcgcctc cagcacgagc agctgcttag gggtgttgga
     3301 gagatggtcc tcgccgcgca cgacgtgggt gatccccgcg tcgaggtcgt cgatcgcgac
     3361 ggcgaagttg tagagcaccg acccgtcggc gcgggcgatc accgggtcgt cgatgtggac
     3421 gtggtcgaag ctggtgtcgc cgcggatgac gtcgtggacc acggtcgcgc cctcgtcggg
     3481 cacgcgcagg cggatcgcgc cgtcgctctc cgggctgccg cggtacccgc gccccggatg
     3541 ctccgccttc caggccttta cctcgtcgga ggcggcgttg gagcggtatg cgtgcccgct
     3601 gtcgagcaac tgcgccagga cgtcgcggtg tcggtcggag cgctcgctct ggaagaccgg
     3661 gccctcgtcg aagtcaagct cgagccagct gagcgcgtcg aggatctgcg ccacgttctc
     3721 ctcggtggag cgctccctgt cggtatcctc gatccgcagc acgaggtcgc ccccggaccc
     3781 gcgggcgaga agccagttgt agagcgccgt gcgcgcgcct ccaatgtgga gggccccggt
     3841 gggcgaagga gcgaatctga ctcggatgga cacgggggcg gcatgctaat cggcgcgcac
     3901 gtatccaccg ccggcggcct cgtcaaggcg catggccgcg gcgtcgagat cggcgccgag
     3961 gcgatccagg tcttcaacca gtcgccgcgg atgtggcgcc cgacgcgctg gaaggacgac
     4021 gacgtcgccg ccttcaggga gctgatggcc gacgggccga tcggctcggt cgtgatccac
     4081 gccgtctacc tgatcaactg cgcgtccaag gacccggaga tccgcgcgaa gtcgcgcacc
     4141 tcgctgatcc actcgctgcg gatgggcgcg gcgatcggcg ccgacggggt cgtgctccac
     4201 cccggctcga ccgtcggcga gccgcacggg gaagcgatcg agcgcgtcgg cgagctgctg
     4261 cgcgaggcgc tcgcggagtc ggagtcgtgc ccgctgctgc tcgaggacac ggcgggagcc
     4321 ggcaacacgc tcggccgctc gttcgaagaa ctggcgcagc tgatcgagct cgccggcggc
     4381 gacgaccgcc tcgggctgtg cctcgactcc tgccacctgc tcgcgagcgg cttcgacgtc
     4441 aggacgcccg cgggcatcga gaaggtgatg gacgactgcg tggcgattgt cggcgagcag
     4501 cggctgcgct gcgtacacgt caacgactca cagacgccgc tcggctcgaa ccgcgaccgc
     4561 cacgccccgc tcggcgacgg cgagctcggc gccgaggggt gcggcgcgtt cctgtcggag
     4621 ccgcgcttcg agggactgcc ggcgatcttc gaggggcccg gcgtcgaggg caaggcgccg
     4681 agcgcggtcg acatcgagcg tgcccatgag ctgcgcagcg ccggcctggc cgctcgcggc
     4741 ctctgagtgt tcgacccgaa cactgtgtaa caaccggcgt cgcggggttg ccctgcgcat
     4801 ctgcagggtc tagcctgcat atctgcatgg gactcgcggc gaaccctgac ttccagaagt
     4861 ggctcgacgg ctacgcggcg cgccacgaac agttgcacgg ggacggtccg acgtcaccgc
     4921 ccggcgtctg cgacggctgc ggcgatccgc tcgccgatcc gccgtcgagc gctcccttct
     4981 gcctcgtctg cctcgccgag cagtacggcc gcgaccgcgc ccaggccgcc gcacggatcg
     5041 caaccgcgct gcggatggcg ctcgccgccg acgacggcac gccggagggc gacatccgtg
     5101 aggccgtcga ggacgtcttg caagacctag cgagctagag gcggtcgcgc aggtcgaagt
     5161 cggcagccgg cttctcgagg aagccgcggt tcacgcactc gaaggcgtcg ccgtggccgt
     5221 cgtaggcacg ctcgacggag gagcgacagc gttcgagcgg ggcgccggtc atcttcactc
     5281 cgccgtagcc gtgccgggtg cgctgcgcgg cgtcgtaggc ggcgacgtcg atctcggcgc
     5341 ggaagacctt gttcaggcag cccatgtagc gggcgccggt ctcctcgtcg tcgtagaggt
     5401 agagcagctc gcagccgctt tcgatgcagc cggccgggtg gacgacgcgg tcgcagaacg
     5461 cgcagcactc acggcactca tgccgatccc cgaggtttgg cagtgccgtc ggctccacgc
     5521 cgctgagggt agcgctgggc cggctacgcg gccatggaga cgcccggcag agcgcccagc
     5581 tcggcgaggc aggcgctgtc ctcagccttg atgcggaagt cgtcgccgag cttgaggctg
     5641 cgctggccga cgcgcagcag cacctggtga tcgccgggga agatttccac gacctgcttg
     5701 agctcgtcga gcaggctctc cgggacgtcg ccggggacgt tgagcgtgac gagcttcgca
     5761 agccgcgtga cctgcggctc ggccgcggcc tcggcggcgg cgagctccac ctcctcgggc
     5821 gacgggtcga aggcggcgac ctcctgcgcg acgagcttga cctcgcccgc ctccttcttg
     5881 tcgacgcggc cgcggacggt gatcaccgag tcgacctcga ccttgtcggc gttgtcggcg
     5941 tagaccttgg cgaagacgag catctcgact tgtccctcga ggtcgtcgag cgtcgcgaac
     6001 agcatcggat cgcccttgcg agtgcggatc ttcttgcact cggtcacgat cccaccgacg
     6061 gtcacccact cctggtcctt gcgctcgggc aggtcggcga tcgagcagtc gaccttgcgc
     6121 cgcagcgcgg cgcggacctc cttgagcgga tggctcgaca ggaacaggcc gagcgtctcc
     6181 ttctcccagg cgttgagctc gggccgctcg tcgggcagcg ccggcaccgg caggtccggt
     6241 tggccggcgg cgccaccgcc gggcgcgggc tcctcgagct cgaagatcga cccctggccg
     6301 agcgcggcgt cctgctgctg cttctggccg gcgccctggg cctgctcgag gacggcgagc
     6361 atccccgagc gggtcgcgcc ggtcgagtcg aaggcgccgc acttgatcag cgcctcggtc
     6421 gccttcctgt tgaccgagcg gcagtcgacg cgtcggcaga agtcccacag cgaagcgaac
     6481 ggtccaccgt cctcgcgcgc cgcgacgatc gcgtcgacgg cagccgagcc gacgttcttg
     6541 acggcgtcga ggccaaagcg gatgttgccc tcgcggacct cgaagttgtg gcccgactcg
     6601 ttgacgtcgg gtggcagcac ctcgatgccc atctcctcgc agcaggagac gaagaacggc
     6661 accttgtcct tggtggacat caccgacgag atcagcgccg ccatgtactc ggccgggtag
     6721 ttggcgcgca gccaggcggt gcggtaggag atcaggccgt agcaggcggc gtgactgcgg
     6781 ttgaacgagt agtcggcggc cgcctcgttg acggcccaga gctcctcgat cacacgctcc
     6841 tgggtcccgc tggcgcgggc gccctcgaag aagcgcgcct tgagcgccgc catcgcctcg
     6901 cggttcttct tgccgatcgc cttgcgcagg tcgtccgcct cggcgccgga gaagccggcg
     6961 accgccttgg cgatctgcat cgactgctcc tggtagagga tcaccgagta cgtcgcctcg
     7021 gtgatcgggc gcaggcgctc gtcgatgtag tggaccgcct cgggattgcg cttgttgcgc
     7081 gcgtaggtgt cgatgtggcg catcgcacca gggcggtaga gcgcgttgag cgcgacgagg
     7141 tcctcgaact cggtcggacg caccttgcgc agcgcgtcgc gcatcccctc ggactcgaac
     7201 tggaagacgc cgaccgagtc gccgcgggcc atcatctcgt aggtcttggg gtcgtcgagc
     7261 ggcagcgacg ccatgtcggg cttctcgcgg ccggactcct cgatgatccg caggcagtcc
     7321 tcgatcacgt cgaggttgcg caggccgagg aagtccatct tcagcaggcc gatctcctcg
     7381 atcggcttca tcgagta
//
LOCUS       DATABV010000195        24035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1428550, whole genome shotgun sequence.
ACCESSION   DATABV010000195 DATABV010000000
VERSION     DATABV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24035
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428550"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VHF88_08820"
     CDS             complement(<1..234)
                     /locus_tag="VHF88_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161907.1"
                     /translation="MEILQEIGSYAGLAAIVGLAVLSALYFSQARDVKRLREWAGRAP
                     DRAPTPAVPAEPRGVVAQPIARPPTRPVPGGTPP"
     gene            complement(250..2094)
                     /locus_tag="VHF88_08825"
     CDS             complement(250..2094)
                     /locus_tag="VHF88_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX2161908.1"
                     /translation="MRRIGQVVVDGCLVALAYYLAYVLRFDEGIPDRYDELLVDTVAF
                     VVVLKLALFAAFGLYSKLWRFVDQADFESILKAVAVSSFALIGLSFLFSPGDVDPPRG
                     VIALDFLLTLALVGGARFAVRALVERQLRRPLVARATTEVLIVGAGNGGQQVAMELRR
                     NPELSTAVIGFVDDDPRKQGMVVGGHRVHGSTDDLPRVLDDTKPDEVIIAIPSAPGML
                     RQKVVTACRERNIPVRTLPTTFELLSRGPNLLRQVRDVRVEDVLGREPVRVEVDRVGA
                     YLTGQVVLVTGAGGSIGSELCRQIAHVKPKRLVLVDHGENNLFEIRRELEDDRHFGNL
                     VAVLADCKDATRMGELFAAECPSVVFHAAAYKHVPLMEENPVEAVRNNAVATRIVAAA
                     AGECGSERFILVSTDKAVAPATVMGASKALAEWAIEAAQNRYHGTRYAAVRFGNVLGS
                     SGSVVPIFRRQIAAGGPVTVTDPNMTRYFMTIPEAVQLIIRSGDLTTGSQVFVLEMGD
                     PVKIVDLARNMIRLSGKEPDVDIAIEIVGRRPGEKIHEELFDPGERPQPTPAEKIVAA
                     VRPRLDPDWVEGAFARIEELVYNGDAAALASAVAELSAERALTAVDAD"
     gene            complement(2178..3224)
                     /locus_tag="VHF88_08830"
     CDS             complement(2178..3224)
                     /locus_tag="VHF88_08830"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX2161909.1"
                     /translation="MRIWIDLTNSPHVLVMRPLVEAMRADGHEVEVTARDFAQTLELC
                     ERLGVEHTAIGSHAGEGLGAKALGLARRSLALARWARGRRFDVALGHASNDVMVAAAL
                     LRIPSATAFDYEWATVQHNVNCRLARAVVVPDAIPPERLHRYGARGKVRGYPGLKEEY
                     YLADFEPDAAVLEELGLEASEPIAVVRTPPDASLYHRHESSVFPRVLERLRDRAQTVV
                     LPRTPRQRQEIAAIAADRSAQDPASSASDAARLIVPDRAIDAQSLIAHADLVVSGGGT
                     MNREAVALGAPVFTTFEGRLGAVDERLLADGRLRRLENPEQVEVVKRAAPSADRIRRD
                     PRVLAEMLLSAAAR"
     gene            complement(3271..4362)
                     /locus_tag="VHF88_08835"
     CDS             complement(3271..4362)
                     /locus_tag="VHF88_08835"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2161910.1"
                     /translation="MAVPLFATSLERQLPALTERLVAVARGGRYILGPEVEAFEREFA
                     DYLGVRHAIGVANGTDAITIALRALGVGPGDEVVCPSFTFYATVEATLSAGATPVYCD
                     VDPQTMCVTAETVRAAITPRTKAIVAVHLFGNVAPMAELRELGLPVLEDAAQATGARL
                     DGVRAGALGDAATFSFFPSKNLPCLGDGGAVATDDDDVAAAVRRLRFHGSDDKKTFVD
                     VGYNSRLDELQAAVLRVLLPQLDGWNAARRAAADSYAREGLGEHVELPMAVDGAEHVH
                     HLYVVRSERADELASALTERGIGARGYYRTPIHRQPATARFAPAGLSLPGTEEVARTH
                     LALPMGPELTDDQVREVATAVAAAGPGAR"
     gene            4452..6167
                     /locus_tag="VHF88_08840"
     CDS             4452..6167
                     /locus_tag="VHF88_08840"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX2161911.1"
                     /translation="MTFDDGTPAAGTPADLGYEPVRRRPGDPLDLVLEHLRRLVRADA
                     VVLMSVDRARQHIEPTASWFSSPELHDALAPSLTRPYDRRRPGLTEAALERGRPLMLP
                     RLEDWEAAGYPRDIVTSTSTERAWELIRRASAICCPIRTPLGRTLGVVTVLSIDPLHP
                     LDKGDLDVVTVIADLAALARERSELLATEAAKAREELLLKRAAEGTSATLEPYEVERQ
                     VVEHALQIAAADHGLLSRVQPGSQRLATVARQGSADVDAATLAEVVRSRSTVSHGGAA
                     PSAHTPIALGPRLFGVLSLIRDDGREFDRDDLMLVSKMARIAAAALANADAFQQERHL
                     ARTLTHGFVPEALPSVSGWDVGVLYEPADDQPTGGDLYGAWPLPDGDVAVVIGDVVGK
                     GVETAALSAMARFFIEARSWDVDDPAVVLEQVNAMLCSRLPSDRFVTAFFGRLGERRL
                     RYANAGHLAPLILRSGGTLSEATDGGLPLGIDPAPGYRVHELELGTDDILLGFTDGIL
                     EARREGKLLGADGLRKIVVDASGMTREPQALTELVHDEVRAWARGLSDDAAIVALRRR
                     DLASG"
     gene            complement(6171..6386)
                     /locus_tag="VHF88_08845"
     CDS             complement(6171..6386)
                     /locus_tag="VHF88_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2161912.1"
                     /translation="MADPLLKFAANVRRLRTEKGLTQDALAQGASMDPAEVRRIESGR
                     RDPGVRVLSRVAAGLGVKPADLLDGVG"
     gene            complement(6509..7984)
                     /locus_tag="VHF88_08850"
     CDS             complement(6509..7984)
                     /locus_tag="VHF88_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2161913.1"
                     /translation="MIGTLLSDRFRLEEKIGAGGMSTVYRAYDVTLERWVAIKSMHRD
                     ISTDPDQLERFRREARAVARLNHPHVVTVIDFGQDDGSPYIVLELVEGETLKQRIKRM
                     GRLPVGEAVAYAIEIGRALSAAHAVRLVHRDVKPQNVLIDQEGRAKVTDFGIARSLEV
                     HGLTATGRVLGTTDYVAPEQALGHEVTEQSDVYSLGIVLYEMLAGEPPFRAESQVAVA
                     MKHVQERMPDIQVARPDVSSSLAAVVDRATAKELGNRYTTAEDLVHDLEQVLAIEAAR
                     AGETTGEATTVLQTLSGDTTEVAPLRLRRPRRWLLSVLAVVVLVAGAIGFLASRTERG
                     PGDVRGVGGGGLRAVTLSGDAAQDYDPQGDEDESSEAANFAIDANPTTVWDTETYEAG
                     FDGANKEGVGLYVDAGSRVAAQRIDVTSSTPGFTAAIYAANDVPDDIEGWTKVSSDLT
                     IGQEQGIDLDTGGQRFRYYLLWIGDLPEGGKATVKELSVLR"
     gene            8007..8873
                     /locus_tag="VHF88_08855"
     CDS             8007..8873
                     /locus_tag="VHF88_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX2161914.1"
                     /translation="MAVRVRCITDPACVWSWAFEPVVRELMVEFGDDLEWTFVMGGLA
                     REYGADAGWGPGGGKPGMAAHWLEATDESGMPTNPLAWSEAPLGSTYPACMAVKAAQE
                     QGRAAAYRYLRALREGILCRRRKLDTTEALVEEARGAGLDVERFRVDLGSHAIVEAFG
                     ADLEEARDVPDVVRDADKVRCSVAVGEERVPFPTLHFEGDGGEPRWTCGMRPYDFVRE
                     QAIAAGARPSGDPRPTVLEAVQRFGTVAAVEVAAICDLPLPRAEAELAQLALDWRIKP
                     MAVLTGRLWELA"
     gene            complement(8870..9817)
                     /locus_tag="VHF88_08860"
     CDS             complement(8870..9817)
                     /locus_tag="VHF88_08860"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX2161915.1"
                     /translation="MRVAVLGGGPSGEHDVSLASAAAIADGLEQAGHEPLRVTIDRDG
                     RWSHQGAPVVLEPAGGLLGCDVAFPALHGPYGEDGVVQGLLEALAIPYVGAGVTASAI
                     CVDKVLFKDAMAVAGVPQVGYAAVRDGDDLEPLGELGLPVFVKPARLGSSVGISKVTV
                     VDELPAALAAAWEHDRLAIVEAMSPGLEIECALIGDAGPQVSIPGEIEIDADWYDYEA
                     KYTEGGMRLIVPARIPEPVAERVRALAADVFRRAGCAGLARCDFFVEDPDGDARILVN
                     ELNTMPGFTPTSVFPKLWQASGLAYPDLLDRLLDLALQD"
     gene            complement(9814..11676)
                     /locus_tag="VHF88_08865"
     CDS             complement(9814..11676)
                     /locus_tag="VHF88_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX2161916.1"
                     /translation="MSDLADGAVVDARYRIVRRIGSGGMADVYLAEDTHLGREVALKV
                     LHRRFAQDAEFVERFRREASAAAGLQHPHVVGVFDRGDHEGTYYIAMEYLSGRTLKDI
                     VASEAPLDQLRVISIGTQILQAAGFAHSHGIVHRDFKPHNVIVDAAGNVKVTDFGIAR
                     AGASEMTETGSIMGTAQYLSPEQAQGHEVTARSDVYSIGVMLYELLTGKLPFEGESAV
                     AIALKHMSDTPPAMNREGLRIEPNLEAVVLGALAKEPAARWQSAEDFAAALEACRPYV
                     EAFLAGDNGQDTAIFAAVPVPPPGDGAQAAREKPRRWPAIALALLVLALIGLMAWVFT
                     RPEQIDVPKVTGMQVTEARDRLDRAGFEEVEVERERSLAELDQVLRQDPDPGEQAARS
                     DTITLVVSDGPGEVRVPSVRGLTRAQAVRELRRSDLRATVTERASANIDRGRAVGTSP
                     AGGETVQRRSNVRLFISTGPQRVTVPNVFGLSLDSATERLDDLGLTYRIEREESDEPE
                     DEVIGQDPEEGASLEPGGTVTLTVAEPPEQVSVPFVTDLSERAALRALRAEGLEGTVQ
                     RRDVEDESQDGRVIEQQPAAGSDVDGGSTVTLIVGRFVAPPPTPTTPEAEAEAP"
     gene            complement(11673..13130)
                     /locus_tag="VHF88_08870"
     CDS             complement(11673..13130)
                     /locus_tag="VHF88_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2161917.1"
                     /translation="MNRQIVQLFALTLLLFGVLIAFTSRWTVFEAESLEDNQLNRRGL
                     IEQQRVPRGLILANDGNTVLARSEPRGRGDRRVYIRSYPEGETFGHPVGYSYINAGET
                     GIERSANDALTGDLNEFGTIFRELSSGEREGDDVVTTLDPDAQRLATELLAGNAGSIV
                     ALEPATGHVRVMASTPGFDPNSVQDPATLRALNRDEGDKPLINRSVQTNYQPGSTFKV
                     VTAAAALDTGKLTPESIVDGSSPQTISGVPLNNAGNADFGSISFTTALTNSVNTVFAR
                     AGEQVGARTLVEYMERFGFYADPQLDYPEGEMIASGIRNSAGRLVKDGFDVGRVAIGQ
                     GGEEGQIQVTPLQMAQVAGAVANGGKLIRPRLTERVVGQDGRVQERTTPAQQAQVMKP
                     ETAEQLTQMMADVVNEGTGTAAALSDVAVAGKTGTAEVSNATLNQVWFIAFAPVDDPQ
                     YAIAVTLEDQPLGTSGGANAAPLAAQVLETLLGGG"
     gene            complement(13144..14598)
                     /locus_tag="VHF88_08875"
     CDS             complement(13144..14598)
                     /locus_tag="VHF88_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2161918.1"
                     /translation="MSARTRELFGLIPVSLLVCAGFAAVLMAQSQEASDLTATYGAVF
                     LGLCLFGHLFIRARLPDADPYLYPLAALLAAFGLVMIYRIDAELAREQAQWFVIGLAL
                     FCATIYLLPDHHVLERYRYTIAAVGIGLLLLPRFPLIGEQVNGAYLAVKLGPIQFQPA
                     EFAKLAIIVFLASYLRDTRDVLVRARLKPLTATAQALLYGIPALFFVLVVLALGLGPL
                     TAVLFGVFVLSAVTLVRERPSLKHFGPLLLIWGLAMLMLIFIRDLGSSLMFFGAFLAL
                     VYVATSRLSIVGVGATLFLGGAAYLGNTVSHVQSRFDTWLDPFDPKLYEATGGSFQIA
                     QSLFAQADGGLFGQGFGQSLLELPGGGTILPAAHTDLIYAVIADELGLFGAAGLVLIY
                     LLFVARGFKTALVASDGFSKLLATGLTAVLALQAFVIVGGVTKVIPLTGVTLPFVSYG
                     GSSILANMVLLALLLIVSDHARRDRDRVGPGGLV"
     gene            complement(14595..15854)
                     /locus_tag="VHF88_08880"
     CDS             complement(14595..15854)
                     /locus_tag="VHF88_08880"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX2161919.1"
                     /translation="MALRVVEQAGLTDVGRQREANEDNLVLDAPVFAVADGMGGARAG
                     EVASQIATEAFRDPRDPGQTPEQQLERVAQEANRRIYELALRDESRRGMGTTLTATLV
                     DGDCVSVGHVGDSRAYRLRDGALTQLTQDHSLVAELERSGQLTPEAAEHHPQRSIITR
                     ALGPEREVEVDTHTHAARSGDVYVLCSDGLTGMISDDELATLVRAAPSLESAAEALVR
                     AANQSGGKDNITVVLFRLEDDGSGPAGDPPTAEETIHQGLSADDVRAAAEERDRDLSR
                     ASSATLPPTAGATSSRSLPRERAGAGRKRPASRSAKARRVLAGVAALAITGVLAVGAY
                     LASRQVYFLGTDDTGFVTVYRGLPYELPLGIDLYSAQYRSGVPAASIPVARRDRVLDH
                     EWRSESDAEDLVRQLELGALDPGRPVQ"
     gene            complement(15857..16348)
                     /locus_tag="VHF88_08885"
     CDS             complement(15857..16348)
                     /locus_tag="VHF88_08885"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2161920.1"
                     /translation="MDEPVAVALKFGFLLVLYLFLLWVARSALRDLSRGVEPVGGGAA
                     QPAPEAGGRLRDDSRNGGMLGSMEPRLEVIAALGHEPGTVFDLSDGATMGRSDHAEIR
                     VDDPFASSAHARIFPRGDFMLVEDMGSTNGTYLNGRQLRGAERLKVADVIRIGDSEYR
                     YQE"
     gene            complement(16363..17142)
                     /locus_tag="VHF88_08890"
     CDS             complement(16363..17142)
                     /locus_tag="VHF88_08890"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX2161921.1"
                     /translation="MSLLRNLESKLGGLVEGAFSRAFKSSVEPVELARKLAKEMEESK
                     MASVSRTYVPNHYRVFLSPDDRSQFATYEPALRKELSDYLLEHARAESLALTSRPVVE
                     FMTDERLALGEFGIQAQLLSPPEEEHEPASAAVAPSQGDFGHTMVYSPDRAGARALAP
                     ERPPRGTGRRAVLAGDGRRTVIEGERFTIGRSRDCDMVIDDPNISRHHCELRAEGDSW
                     RIADLGSTNGIKVNRRRVDEALLRSGDRIALGLTDLDFELE"
     gene            complement(17156..18853)
                     /locus_tag="VHF88_08895"
     CDS             complement(17156..18853)
                     /locus_tag="VHF88_08895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2161922.1"
                     /translation="MSRSQTTRRIAGDAHTERLDDARTERLGGPPSGRVVLGRYRLGE
                     RLGAGGFGVVWRAHDERLERDVAVKVVPFGGDAGDEARIQREAVAAARLNHPGIVALY
                     ELGHDEHAAYLVSELVEGATLADLTREGAVSDRDVAKIGAALCEALEHAHARGVVHRD
                     VKPGNVMVLAEPAAGAGFAKLTDFGIARLATGDALTATGDIVGTLAYMAPEQAEGGAV
                     GPEGDVYSLALTLYEAWTGSNPVRGASPAATARQVGAPLPPLRRRRRDLPAALCEVVD
                     AALDPDPGYRPTLAELREVLVDTEQELPDEGGLVEPEVIERFGLTAVRPRTRLTRVLR
                     RSRRPPPIVVPGEEEHATREGWRDAIVGRVASGAAAAILVTLGIGLGPDPSFAVGTVA
                     LVAAGAVALLPRIGWIAAALAVCGWLASPEGGQEGTALVLTAALLPVPLLLPRAGRLW
                     SAGALAPLLGAIGLAPLFVLAAAWLGGTVWRRAGLAAAGLLWLRFTEALTGADLLYGS
                     APATPPRAGWEGSVVDAAREAVEPVVTSVDPVLAIAWVGAAVALPLLARVRRGPALP"
     gene            19075..20073
                     /locus_tag="VHF88_08900"
     CDS             19075..20073
                     /locus_tag="VHF88_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161923.1"
                     /translation="MVRRSLALGAGLLVLILLIFGVRSCMDNQKESAITDWVRDVDAL
                     MKQSNGESEALFDQLGGGGGTDVDVENALNSFRIQSAQLVDQANGLDRPGELDRAQEE
                     LVETLTFRAEGIAQIADALPNALTDGEQAEGAARRIAEAMQLFVTSDQIYFKRVVPAL
                     DEVLEQEGLLLSLTKSTFLPDLSWLDPAEVADRLSGIASGGGEEGDEEAAPGLHGNGL
                     GTVTLGGQTLVAGGSTSVTATGDLSFEVQVINQGENTETDVTVTATVGSGADEIVAEG
                     VIDTIAAGESQVVTIRLEETPPTGQAVPITIEIELVPGEDADIGNNSGEFSVIFTS"
     gene            20094..20609
                     /locus_tag="VHF88_08905"
     CDS             20094..20609
                     /locus_tag="VHF88_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX2161924.1"
                     /translation="MDGLTTTEGVVALAGAGLALIALLLVLVLALRLRRLRADQRAVL
                     GAGGQRDLVEHAARLERGFVELRDWVEDTAAGIETRMGAAEARIDGCVAHRALVRYDA
                     YGEMSGQQSSSLALLDARRSGVVISSIHHRDSARVYFKQVVAGESELELSPEEAEAVE
                     LALAGAGQAAQ"
     gene            20606..21460
                     /gene="pheA"
                     /locus_tag="VHF88_08910"
     CDS             20606..21460
                     /gene="pheA"
                     /locus_tag="VHF88_08910"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX2161925.1"
                     /translation="MSDAPRRVAYLGPPGTNTDEALRASATEPFEAVACTTVPDAIMA
                     VQEGTVDRAVVPIENSLEGGVAATLDALAGEAADVRIVRETVHPIHHHLLAAGPLELG
                     AVRRVLSHPQALGQCSRFLREQLPQAEIAAASSTAEAVRSLSEADEPWAAIGSRLAGE
                     LYGATIVAERIEDRSDNVTRFVWLAPADAAATPADGPAKTSIVFWGFNDAAPGALVSV
                     LRELSDREINLTKIESRPRRVRLGHYMFFADLEASAAEPRVEEALAALGERVETLRIL
                     GSYPASPH"
     gene            21598..22095
                     /locus_tag="VHF88_08915"
     CDS             21598..22095
                     /locus_tag="VHF88_08915"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX2161926.1"
                     /translation="MLVLNASYEPINVCTVRRAAVLILKDRAEILERSDWALHAESFT
                     LPRPMVIRLKTYVRVPRDAHRRKITRRAVFARDQWTCQYCGAERGSLTVDHVIPRSKG
                     GPTDWHNVVTCCAPCNRRKGDRLPRQANMVPRHPPSAPNSTIFIHVAVPVIPQSWERY
                     IPSAA"
     gene            22100..22663
                     /locus_tag="VHF88_08920"
     CDS             22100..22663
                     /locus_tag="VHF88_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161927.1"
                     /translation="MESSNPPKPPGQPAGSSPPPTPPSEQPTKPIPPADAAAARRTDA
                     AVVEHAVEEAIEHDGLRTVRRWMIVLSIWALAATAIAVIAFMEARDAKTEADSAQSAT
                     QANIERVQQRLRDDVDQLRTDVDRLPTQADVEQLQTRVRKAQRDATAARDDATAATSD
                     AAGLRPDIEELERQVNSLRGVVNARGD"
     gene            complement(22978..>24035)
                     /locus_tag="VHF88_08925"
     CDS             complement(22978..>24035)
                     /locus_tag="VHF88_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX2161928.1"
                     /translation="PLATYRKQHRGGVGVRGMDMKDEDYIEHLFVCSTHDYLLFFTNR
                     GKVYRQKVYELPEASRTAKGRALVNVLPLREGEFVRAVLSTRDFREGKYLVFATKQGQ
                     IKKTEFLAYNTPIKADGIIAIKIRDDDELVAVRRTSGDDDIIMISRSGQAARFNEEQA
                     RPMGRDTGGVRGMNVSRGDNCVLAMDIARDDTELLVVTENGYGKRTPIAEYPVKGRGA
                     MGVKTINITEKKGGVAGALIVREHQDLVFISQNGMVQRTGVRGISQQGRPAQGVRVMN
                     LREDDIVSAVALVMEDEADTAAVVDGAPDGLEPIAELTSPDDPETAGVAEPDESDISG
                     DVAPTENDAEDDQPDAE"
BASE COUNT         3401 a         8736 c         8613 g         3285 t
ORIGIN      
        1 tggcggcgtc cccccgggca ccgggcgcgt cggcggacgg gcgatcggct gtgccaccac
       61 cccgcgcggc tcggccggga ccgctggcgt cggcgcacgg tcaggtgccc gccccgccca
      121 ctcgcgcaga cgcttgacgt cgcgcgcctg cgagaagtag agggcggaga ggacggcgag
      181 gccgacgatg gccgcgaggc ccgcgtacga gccgatttcc tggaggatct ccatcgaagt
      241 tcgtgaactc tagtccgcgt cgacggcggt gagggcgcgc tcggccgaca gctcggccac
      301 ggcgctggca agggccgctg cgtcgccgtt gtagacgagc tcctcgatcc gcgcgaaggc
      361 accctcgacc cagtcggggt cgaggcgggg ccgcacggcg gcgacgatct tctcggcggg
      421 ggttggctgc ggccgctcgc cggggtcgaa gagctcctcg tgaatcttct cgcccgggcg
      481 ccgcccgacg atctcgatcg cgatgtcgac gtcgggctcc ttgcccgaca gccggatcat
      541 gttgcgcgcc aggtcgacga tctttaccgg gtcgcccatc tcgagcacga agacctgcga
      601 gccggtcgtc aggtcgccgg agcggatgat cagctggacc gcctcgggaa tcgtcatgaa
      661 gtagcgcgtc atgttcgggt cggtgaccgt caccggcccg ccggcggcga tctggcgacg
      721 gaagatcggg acgaccgagc ccgacgagcc gagcacgttg ccgaagcgca cggccgcgta
      781 acgggtgccg tggtagcggt tctgcgccgc ctcgatcgcc cactcggcaa gtgccttgga
      841 agcacccatc acggtcgccg gcgcgaccgc cttgtcggtc gagacgagga tgaagcgctc
      901 gctgccgcac tcgccggcgg ccgcggcgac gatccgcgtc gcgacggcgt tgttgcgcac
      961 cgcctcgacc gggttctcct ccatcagcgg gacgtgcttg taggcggcag cgtggaagac
     1021 gaccgacggg cactcggcgg cgaacagctc gcccatccgc gtcgcgtcct tgcagtcggc
     1081 gagcaccgca accaggttgc cgaagtggcg gtcgtcctcg agctcgcggc ggatctcgaa
     1141 gaggttgttc tcgccgtggt cgacgagcac cagccgcttc ggcttgacgt gcgcgatctg
     1201 gcggcagagc tccgaaccga tcgatccgcc ggcgccggtc acgagcacga cctggccggt
     1261 gaggtaggcg ccgacacggt cgacctcgac gcgcaccggc tcgcggccga gcacgtcctc
     1321 gacccggacg tcgcggacct ggcgcagcag gttcgggccg cgcgagagca gctcgaaggt
     1381 cgtcggcagc gttcgcaccg ggatgttgcg ctcgcggcag gcggtgacga ccttctggcg
     1441 cagcatgccg ggcgcggagg ggatcgcgat gatcacctcg tcgggcttgg tgtcgtcgag
     1501 cacgcgcggc aggtcgtcgg tgctgccgtg gacgcggtgc ccgccgacga ccatcccctg
     1561 cttgcgcggg tcgtcgtcga cgaagccgat caccgcggtc gagagctcgg ggttgcggcg
     1621 cagctccatc gcgacctgct ggccgccgtt gccggcgccg acgatcagca cctcggtcgt
     1681 cgcgcgtgcg accagcggac ggcgcagctg gcgctcgacc agcgcgcgaa cggcgaagcg
     1741 ggcgccgccg accagcgcca gcgtcagcag gaagtcgagt gcgatcacgc cgcgcggcgg
     1801 gtcgacgtcg ccgggcgaga agaggaagct gagcccgatc agcgcgaagc tcgagaccgc
     1861 aaccgccttg aggatcgact cgaagtcggc ctggtcgacg aagcgccaga gcttcgagta
     1921 gaggccgaag gcggcaaaca gcgccagctt gaggacgacg acgaaggcga ccgtgtcgac
     1981 cagcagctcg tcgtagcggt ccgggatccc ctcgtcgaag cgcaggacgt aggcgaggta
     2041 gtaggcgagc gccaccaggc agccgtcgac gacgacctgt ccgatccggc gcaccccgac
     2101 cgaggatgtg agacgggagg gcaagaggcc cagggtaccg ccgggggcgg cgcgcggggc
     2161 gtttggagcg gggccgctca gcgagcggcg gcggagagca gcatttcggc caggacgcgc
     2221 gggtcgcggc ggatgcgatc ggcggagggg gcggcgcgct tgaccacctc gacctgctct
     2281 gggttctcga gccggcgcag gcggccgtcg gcgagcagcc gctcgtcgac cgcgccgagc
     2341 cgcccctcga acgtcgtgaa gaccggcgcc ccgagcgcga ccgcctcgcg gttcatcgtc
     2401 ccgccgccgg agacgacgag gtcggcgtgg gcgatcaggc tctgggcgtc gatcgcgcgg
     2461 tcgggaacga tcaggcgggc ggcgtccgaa gccgacgagg ccgggtcctg agcggagcga
     2521 tcggcggcga tcgccgcgat ctcctggcgc tggcgtgggg tgcgcggcag cacgacggtc
     2581 tgagcgcgat cccggaggcg ctcgagcaca cgcgggaaca ccgagctctc gtggcggtga
     2641 tagagcgacg cgtccggcgg cgtgcgaacc accgcgattg gctcgctcgc ctcgagcccg
     2701 agctcctcga gcaccgccgc gtcgggctcg aagtcggcga ggtagtactc ctccttcagc
     2761 ccggggtaac cgcggacctt gccgcgcgcg ccgtagcggt ggagccgttc cggcggaatc
     2821 gcatccggca cgacgaccgc ccgtgcgagg cggcagttca cgttgtgctg caccgtcgcc
     2881 cactcgtagt cgaacgcggt cgccgagggg atccgcagca gcgccgcggc aaccatcacg
     2941 tcgttggacg cgtggccgag cgctacgtcg aagcgccggc cacgcgccca gcgggcgaga
     3001 gcgaggctac ggcgcgccag cccgagcgcc ttcgcgccca acccctcgcc ggcgtggctc
     3061 ccgatcgccg tgtgctcgac cccgagccgc tcgcacagtt ccagggtctg ggcaaagtcg
     3121 cgcgccgtca cctctacctc gtgaccgtcc gcgcgcatcg cctcgaccag cggccgcatc
     3181 accaacacgt gcgggctgtt cgtcaggtcg atccagatgc gcatgtaacc gctcggtgag
     3241 ggattagacc gtccaggcga tgcgaatcca tcatctggcg ccgggacccg cggccgccac
     3301 ggcggtggcg acctcacgga cctggtcgtc ggtcagctcg ggacccatcg gaagcgccag
     3361 gtgggtccgg gcgacctcct cggtgcccgg gagggacaac cccgccggcg cgaagcgcgc
     3421 agtcgccggc tggcggtgga tcggcgtgcg gtagtagccg cgcgcgccga tgccgcgctc
     3481 ggtcagcgcg gacgcgagct cgtctgcgcg ctcgctgcgg acgacgtaga ggtggtgcac
     3541 atgctcggcg ccgtcgaccg ccatcggcag ctccacgtgc tccccgagcc cctcgcgcgc
     3601 ataagaatcg gccgccgcac gccgcgccgc attccacccg tcgagctgcg gcagcagcac
     3661 gcgcagcacc gcggcctgca gctcgtcgag gcgcgagttg tagccgacgt cgacgaacgt
     3721 cttcttgtcg tccgagccgt ggaagcgcag ccgccgcacc gcggccgcga catcgtcgtc
     3781 gtcggtggcg accgccccac cgtcaccgag gcaaggcagg ttcttcgagg ggaagaagga
     3841 gaaggtggcc gcatcgccga gcgccccggc ccgcaccccg tcgagccgtg cacccgtcgc
     3901 ctgcgcggcg tcctccagca cgggcagccc cagctcgcgc agctccgcca tcggcgcgac
     3961 gttgccgaac aggtggacgg cgacgatcgc cttcgtgcgc ggagtgatcg ccgcgcgcac
     4021 ggtctcggcc gtcacgcaca tcgtctgcgg gtcgacatcg cagtagaccg gcgtcgcccc
     4081 agccgagaga gtcgcctcga cggtcgcgta gaaggtgaac gaggggcaga cgacctcgtc
     4141 gccgggtccg accccgagcg cccgcaacgc gatcgtgatc gcgtctgtcc cgttcgccac
     4201 gccgatcgcg tgacggacgc caaggtagtc ggcgaactcg cgctcgaagg cctcgacctc
     4261 cggcccgagg atgtagcggc cgccccgcgc gaccgccacg agccgctccg tcaatgccgg
     4321 cagctgccgt tcgagactgg tcgcgaagag gggcacggcc atggccgcaa gaccctactg
     4381 cgcacgacac gggtccgatc tcggctgact tctggcggtg gcgggcggtc ggacctacgc
     4441 tgggccgcag catgaccttc gacgacggca cccccgcagc cggcaccccg gccgaccttg
     4501 gctacgagcc ggtccgccgc cggccgggcg atccgctcga cctcgtgctc gagcacctgc
     4561 gccgtctcgt gcgcgccgac gcggtcgtgc tgatgtcggt cgatcgagcg cgccagcaca
     4621 tcgagccgac ggcgtcctgg ttctcgtcgc cggagctcca cgacgcgctc gccccgtcgc
     4681 tcacccgccc ctacgatcgc cgccggccgg gactcaccga ggcggcactg gagcgcgggc
     4741 ggccgctgat gctgccgcgg ctcgaggact gggaggccgc cggctatccg cgcgacatcg
     4801 tcacctcgac atcgaccgag cgcgcctggg agctgatccg ccgggcgtcg gcgatctgct
     4861 gcccgatccg gaccccgctg ggccgcacgc tcggcgtggt caccgtcctg tcgatcgatc
     4921 cgctccaccc gctcgacaag ggcgacctcg acgtcgtgac ggtgatcgcc gacctcgcgg
     4981 cgctggcccg cgagcgctcg gagctgctcg ccaccgaggc ggcgaaggcg cgcgaggagc
     5041 tgctgctcaa gcgggctgcg gaggggactt cggccacgct cgagccgtac gaggtcgaac
     5101 gccaggtggt cgagcacgcg ctccagatcg ccgccgccga ccacggcctg ctgagtcgcg
     5161 tccagcccgg gtcgcagcgc ctggcgaccg tcgcgcgcca ggggtccgcc gacgtcgacg
     5221 cggcgactct cgccgaggtc gtccgatcgc gcagcaccgt ctcgcacggc ggcgccgcgc
     5281 cgtcggcgca cacgccgatc gcgctcgggc cgcgactgtt cggggtgctg tcgctgatcc
     5341 gcgacgacgg gcgcgagttc gaccgcgacg acctgatgct cgtgtcgaag atggcgcgga
     5401 tcgcggcggc tgcgctcgcg aacgccgacg ccttccagca ggagcggcac cttgcgcgca
     5461 cgctgacgca cggcttcgtc cccgaggcgc tgccgagcgt gtcgggctgg gacgtcggcg
     5521 tcctctacga gccggccgac gaccagccga ccggcggcga cctctacggc gcatggccgc
     5581 tgcccgacgg cgacgtcgcc gtggtgatcg gagacgtggt cggcaagggc gtcgagacgg
     5641 cggcgctgag cgcgatggcg cgcttcttca tcgaggcgcg cagctgggac gtcgacgatc
     5701 ccgcggtggt gctcgagcag gtcaacgcga tgctctgcag ccggctgccg agcgaccgct
     5761 tcgtgacggc gttcttcggt cggctcgggg aacggcggct gcgctacgcc aacgccggtc
     5821 atctcgcccc gctgatcctg cggtcgggcg ggacgttgtc cgaggccacc gacggcggcc
     5881 tgccgctggg gatcgacccc gcgcccggct accgcgtcca cgagctcgag ctcggcaccg
     5941 acgacatcct gctcggcttc accgacggga tcctcgaggc gcggcgcgag gggaagctgc
     6001 tcggagcgga cggcttgcgc aagatcgtcg tcgatgccag cgggatgacg cgcgagccgc
     6061 aggcgctgac cgagctcgtc cacgacgagg ttcgggcctg ggcccgtggc ctcagcgacg
     6121 acgccgcgat cgtcgcgctg cgtcgccgcg acctcgctag cggctaacga ctaaccgacg
     6181 ccgtcgagga ggtcggccgg cttgacgccg aggccggcgg cgacacgcga cagcacgcgc
     6241 acacctgggt cgcggcggcc ggactcgatc cggcggacct cggcgggatc catcgaggcg
     6301 ccctgggcga gcgcgtcctg cgtcaggccc ttctccgtcc gcagacggcg cacgttcgcc
     6361 gcgaacttga ggagcggatc ggccacgagc gggactgttc cgcctggcag gacgcggcac
     6421 caggacttat cgctcctgca ccgctctacc ggcggcagag acgtagctgt atatgcggca
     6481 gcgtcggtac gccgccttac cgtcgcggct agcggaggac cgacagctcc ttgaccgtcg
     6541 ccttgccgcc ctccggcaga tcgccgatcc agagcaggta gtagcggaag cgctggccgc
     6601 cggtgtcgag gtcgatcccc tgctcctggc cgatcgtcag gtcgctgctg accttcgtcc
     6661 acccctcgat gtcgtcgggc acgtcgttcg ccgcatagat ggcggcggtg aagccggggg
     6721 tcgagctcgt cacgtcgatt cgctgcgccg ctacgcgcga gcccgcgtcg acgtacaggc
     6781 cgaccccctc cttgttcgca ccgtcgaagc cggcctcgta ggtctcggtg tcccagaccg
     6841 tcgtcgggtt ggcgtcgatc gcgaagttgg ccgcctccga cgactcgtcc tcgtcgccct
     6901 ggggatcgta gtcctgcgcc gcatcgccgc tcagcgtcac cgcgcgcaat ccaccgccgc
     6961 cgacgccccg gacgtcgccg ggaccgcgct cggtacggct ggccaggaag ccgatcgcac
     7021 cggccaccag cactaccacg gcaagcaccg acagcagcca gcggcgcggg cggcgcagtc
     7081 gcagcggcgc gacctcggtc gtgtcgccgg acagcgtctg caggaccgtc gtcgcctcgc
     7141 cggtcgtctc gcccgcgcgc gccgcctcga tcgccagcac ctgctcgagg tcgtggacga
     7201 gatcctcggc ggtcgtgtag cggttgccga gctccttggc ggtcgcgcgg tcgacgacag
     7261 ccgccagcga cgacgagacg tcggggcgcg cgacctggat gtcgggcatc cgctcctgga
     7321 cgtgcttcat cgccaccgcg acctgactct cggcgcggaa cggcggctcg ccggcgagca
     7381 tctcgtagag gacgatcccg agcgagtaga cgtcggactg ctcggtcacc tcgtggccga
     7441 gcgcctgctc gggcgcgacg tagtcggtcg tgccgagaac gcgaccggtc gcggtcaggc
     7501 catggacctc gagcgagcgg gcgatgccga agtcagtgac cttcgcgcgg ccctcctggt
     7561 cgatcagcac gttctgcggc ttcacgtcgc ggtggacgag ccgcaccgcg tgggctgccg
     7621 acagcgcccg cccgatctcg atcgcatagg caacggcctc gccgaccggc agccgtccca
     7681 tccgcttgat cctctgcttg agcgtctcgc cttcgaccag ctcgagcacg atgtagggcg
     7741 agccgtcgtc ctggccgaag tcgatgaccg tgacgacgtg cgggtggttg aggcgcgcga
     7801 cggcgcgcgc ctcgcgccgg aaccgctcga gctgatccgg gtcggtcgag atgtcgcgat
     7861 gcatcgactt gatcgcgacc cagcgctcga gcgtgacgtc gtaagcgcgg tagaccgtcg
     7921 acatcccgcc cgcgccgatc ttctcctcga gacggaagcg gtcgctgaga agggtgccga
     7981 tcactgtccc tattgtgcgg tcttcgatgg cggtacgcgt tcgttgcatc acagaccccg
     8041 cctgcgtctg gtcgtgggcc ttcgagccgg tggtccggga gctcatggtg gagttcggcg
     8101 acgacctcga atggaccttt gtgatgggcg gtctggcgcg tgagtacggg gccgacgcgg
     8161 gctggggtcc gggcggcggc aagcccggca tggcggcgca ctggctcgag gccaccgacg
     8221 agtccgggat gccgacgaac ccgctcgcct ggagcgaggc gccgctgggt tccacctacc
     8281 ccgcctgcat ggccgtcaag gccgcgcagg agcagggccg agctgcggcc taccgctacc
     8341 tgcgcgccct gcgcgagggg atcctctgcc gtcgccgcaa gctcgacacc accgaggcgt
     8401 tggtcgagga ggcgcgcggg gccgggctcg acgtcgagcg ctttcgcgtc gatctcggct
     8461 cgcacgcgat cgtcgaggcc ttcggcgccg acctcgagga ggcgcgcgac gtccccgacg
     8521 tggtgaggga cgcggacaag gtgcgctgct ctgtcgccgt cggggaggag cgcgtgccgt
     8581 tcccgaccct gcacttcgag ggggacggcg gcgagccgcg ctggacctgc ggcatgcggc
     8641 cctacgactt cgtccgcgag caggcgatcg cggccggcgc tcggccgtca ggtgacccga
     8701 ggcccaccgt gctcgaggcg gtccagcgct tcggcacggt cgccgccgtc gaggtcgccg
     8761 ccatctgcga cctgccgctc ccccgcgccg aggccgagct cgctcagctc gcgctcgact
     8821 ggaggatcaa gccgatggcg gtcctcaccg gacggctctg ggagctcgcc tagtcctgga
     8881 gggccagatc cagcaggcgg tcgagcaggt cgggataggc gaggccgctg gcctgccaga
     8941 gcttcgggaa gaccgaggtc ggggtgaagc cgggcatcgt gttgagctcg ttgacgagga
     9001 tgcgggcgtc gccgtcgggg tcctcgacga agaagtcgca gcgggccagg ccggcgcagc
     9061 cggcgcggcg gaagacgtcg gcggcgagcg cccggacgcg ctcggcgacg ggctcgggga
     9121 tgcgcgcggg gacgatcagc cgcatgccgc cctcggtgta cttcgcctcg tagtcgtacc
     9181 agtcggcgtc gatctcgatc tcgcccggaa tcgatacctg cggcccggcg tcgccgatca
     9241 gcgcacactc gatctcgagg ccgggcgaca tcgcctcgac gatcgccagt cgatcgtgct
     9301 cccacgccgc cgcgagggct gccggcagct cgtcgacgac ggtcaccttc gaaatgccga
     9361 ccgacgaccc gagccgcgcc ggcttgacga agaccggcag gccgagctcg ccgagcggct
     9421 ccaggtcgtc gccgtcgcgc accgccgcgt agccgacctg gggcacgccg gcgacggcca
     9481 tcgcgtcctt gaagaggacc ttgtcgacgc agatcgcgct cgcggtgaca cccgcgccga
     9541 cgtacgggat cgcgagcgcc tcgagcagcc cttggaccac cccgtcctcg ccgtaggggc
     9601 cgtgcagtgc ggggaacgcg acgtcgcagc cgagcagccc gccggcgggc tcgagcacga
     9661 ccggcgcgcc ctggtgcgac cagcggccgt cgcggtcgat cgtcacacgc aacggctcgt
     9721 ggccggcctg ctcgaggccg tcggcgatcg cggccgcgga ggcgagcgag acgtcgtgct
     9781 cgcccgacgg accgccgccg agcacggcga ccctcacggc gcctccgcct ccgcctcggg
     9841 cgtcgtcggc gtcggcggcg gcgcgacgaa gcgcccgacg atcagcgtga cagtgctgcc
     9901 gccatcgacg tcgctgccgg cggcgggctg ctgctcgatc acccgcccgt cctgactctc
     9961 gtcctcgacg tcgcgccgct gcaccgtccc ctcgaggccc tcggcgcgaa gggctcgcag
    10021 cgcggcccgc tccgagaggt cggtgacgaa cggcaccgac acctgctcgg ggggctccgc
    10081 gacggtcagc gtgacggtgc cgcctggctc gagcgaggcg ccctcctcgg ggtcctgccc
    10141 gatcacctcg tcctccggct cgtcggactc ctcgcgctcg atccggtagg tcagcccgag
    10201 gtcgtcgagc cgctcggtcg ccgagtcgag cgacaggccg aagacgttcg ggaccgtgac
    10261 gcgctggggc cccgtactga tgaatagccg cacgttcgac cgccgctgaa cggtctcacc
    10321 gccggccggc gaggtcccga ccgcccgccc gcggtcgatg ttcgccgacg cccgctcggt
    10381 gacggtcgcg cgcaggtcgg agcggcgcag ctcgcggacg gcctgcgcgc gcgtcaggcc
    10441 gcgcaccgac ggcacgcgca cctcgcccgg gccgtccgac acgaccagcg tgatcgtgtc
    10501 cgagcgcgcc gcctgctcgc cggggtctgg gtcctggcgc agcacctggt cgagctcggc
    10561 gagcgagcgc tcgcgctcga cctcgacctc ctcgaagccg gcgcggtcga gccggtcgcg
    10621 cgcctcggtc acctgcatgc cggtgacctt cgggacgtcg atctgctccg gacgcgtgaa
    10681 cacccaggcc atcagcccga tcagcgcgag caccagcagc gcaagcgcga tcgccggcca
    10741 gcggcgcggc ttctcgcggg cggcctgcgc gccgtcgccc ggcggcggga ccggcaccgc
    10801 cgcgaagatc gcagtgtcct ggccgttgtc gccggcgagg aacgcctcga cgtagggccg
    10861 gcaggcctcg agcgcggccg cgaagtcctc agccgactgc cagcgcgcgg ccggctcctt
    10921 ggcgagcgcg ccgagcacga ccgcctcgag gttcggctcg atccgcaggc cctcgcggtt
    10981 catcgccggc ggcgtgtccg acatgtgctt gagcgcgatc gcgaccgcgc tctcgccctc
    11041 gaacggcagc ttgccggtca gcagctcgta gagcatcacg ccgatcgagt agacgtcgga
    11101 gcgagccgtc acctcgtggc cctgcgcctg ctccggcgag aggtactggg cggtgcccat
    11161 gatcgatccc gtctcggtca tctcggaggc gccggcgcgg gcgatgccga agtcggtcac
    11221 cttgacgttg ccggctgcgt cgacgatcac gttgtgcggc ttgaagtcgc ggtggacgat
    11281 gccgtggctg tgcgcgaaac cggccgcctg caggatctgc gtgccgatcg agatcacccg
    11341 cagctggtcg agcggcgcct ccgacgcgac gatgtccttg agcgtgcggc cgctcaggta
    11401 ctccatcgcg atgtagtagg tcccctcgtg gtcgccgcgg tcgaagacgc cgacgacgtg
    11461 cggatgctgg agcccagccg ccgccgacgc ctcgcgccgg aagcgctcga cgaactccgc
    11521 gtcctgggcg aagcggcggt gcagcacctt gagcgcgacc tcgcggccga ggtgggtgtc
    11581 ctcggcgagg tagacgtcgg ccatccctcc cgagccgatc cggcggacga tgcggtagcg
    11641 cgcgtcgacg acggcgccgt cggccaggtc gctcatccac cccccagcag cgtctcgagc
    11701 acctgggcgg cgagcggagc ggcgttcgcc ccacccgacg tgccgagcgg ctggtcctcg
    11761 agcgtcaccg cgatcgcgta ctgcgggtcg tccacgggcg cgaaggctat gaaccagacc
    11821 tggttcagcg tcgcgttgct gacctcggcg gtgccggtct tgccggcgac ggcgacgtcg
    11881 gacagcgcag cggcggtgcc ggtgccctcg ttgacgacgt cggccatcat ctgcgtcagc
    11941 tgctccgcgg tctcgggctt catcacctgc gcctgctgcg cgggtgtggt ccgctcctgc
    12001 acgcggccgt cctgaccgac gacgcgctcg gtcagccgcg ggcggatcag cttgccgccg
    12061 ttggcgactg cgccggcgac ctgggccatc tgcagcggcg tcacctggat ctggccctcc
    12121 tcgccgccct ggccgatcgc cacgcggccg acgtcgaagc cgtccttgac cagccggccg
    12181 gccgagttgc ggatcccgct cgcgatcatc tcgccctccg ggtagtcgag ctgcgggtcg
    12241 gcgtagaagc cgaagcgctc catgtactcg accagcgtcc gcgcgccgac ctgctcgccg
    12301 gcgcgtgcga agaccgtgtt gaccgagttg gtcagcgcgg tcgtgaagct gatcgagccg
    12361 aagtcggcgt tgccggcgtt gttgagcggg acgccgctga tcgtctgcgg cgacgagccg
    12421 tcgacgatcg actccggcgt cagcttgccg gtgtcgagcg ccgcggccgc ggtgaccacc
    12481 ttgaaggtcg agcccggctg gtagttggtc tggaccgagc ggttgatcag cggcttgtcg
    12541 ccctcgtcgc ggttcagcgc gcgcagcgtc gccgggtcct ggaccgagtt cgggtcgaag
    12601 ccgggcgtcg aggccatcac gcgcacgtgg ccggtcgccg gctcgagcgc gacgatcgag
    12661 ccggcgttgc cggcgagcag ctcggtcgcc agccgctggg cgtccgggtc gagcgtcgtg
    12721 acgacgtcgt cgccctcgcg ctcgccgctc gacagctccc ggaagatcgt gccgaactcg
    12781 ttcaggtcgc cggtcagcgc gtcgttggcc gaccgctcga tccccgtctc gcccgcgttg
    12841 atgtacgagt agccgaccgg gtggccgaac gtctcgcctt ccgggtagga gcggatatag
    12901 acgcgccggt cgccgcggcc gcgcggctcg ctgcgcgcca gcaccgtgtt gccgtcgttc
    12961 gcgaggatca gcccgcgcgg gacgcgctgc tgctcgatca gcccgcgccg gttgagctgg
    13021 ttgtcctcga gcgactcggc ctcgaagacg gtccagcgcg acgtgaaggc aatcagcacc
    13081 ccgaacagca ggagcgtcag cgcgaacagc tgcacgatct gacggttcac gcgtccacct
    13141 cgctcacacc aacccgccgg ggccgacgcg gtcgcggtcg cggcgggcgt ggtcggagac
    13201 gatcagcagc agcgccagca gcaccatgtt ggcgaggatc gagctgccgc cgtaggagac
    13261 gaacggcagc gtgacgccag tcagcgggat caccttcgtg acgccgccga cgatcacgaa
    13321 ggcctgcagc gcgaggaccg cagtcaggcc ggtcgcgagc agcttcgaga acccatcgct
    13381 cgcgaccagc gccgtcttga acccgcgcgc gacgaacagc aggtagatca gcaccaggcc
    13441 cgccgcgccg aacaggccga gctcgtcggc gatcaccgcg tagatcaggt cggtgtgcgc
    13501 cgccggcagg atcgtcccgc cgcccggcag ctcgagcagc gactggccga agccctggcc
    13561 gaacaggccg ccgtcggcct gggcgaacag cgactgggcg atctggaagc tgccgccggt
    13621 cgcctcgtag agcttcgggt cgaacgggtc gagccaggta tcgaagcggc tctggacgtg
    13681 gctgaccgtg ttgccgaggt atgccgcccc gccgaggaac agcgtcgccc cgacgccgac
    13741 gatcgacagc cgcgacgtcg ccacgtacac cagcgcgagg aaggcgccga agaacatcag
    13801 cgagctgccg aggtcgcgga tgaagatcag catcagcatc gccaggcccc agatcagcag
    13861 cagcggcccg aagtgcttca gcgacggacg ctcgcggacc agcgtgaccg ccgacaggac
    13921 gaagacgccg aacagcacgg cggtcagcgg gccgagcccg agcgccagca ccaccaacac
    13981 gaagaagagc gccgggatgc cgtagagcag cgcctgggcg gtggcggtga gcggcttcag
    14041 ccgagcccgc acgagcacgt cgcgcgtgtc gcgcagatag ctcgcgagga agacgatgat
    14101 cgcgagcttc gcgaactccg ccggctggaa ctggatcggc ccgagcttga cggcgaggta
    14161 ggcgccgttg acctgctcgc cgatcagcgg gaagcgcggc agcagcagca gcccgatccc
    14221 gaccgctgcg atcgtgtagc ggtagcgctc gaggacgtgg tggtcgggca gcaggtagat
    14281 cgtcgcgcag aacagcgcca gcccgatcac gaaccactgc gcctgctcgc gtgccagctc
    14341 ggcgtcgatc cggtagatca tcaccaggcc gaacgccgcc agcagcgcgg cgagcggata
    14401 gaggtacgga tctgcgtcgg gcagccgcgc gcggatgaac aggtggccga agaggcacag
    14461 cccgaggaag accgcgccgt aggtcgcggt caggtcgctc gcctcctgcg actgcgccat
    14521 cagcacggcg gcgaacccgg cgcagacgag cagcgagacc gggatcaggc cgaacagctc
    14581 gcgcgtgcgc gcgctcactg cacgggccgc ccggggtcga gcgcgccgag ctcgagctga
    14641 cggacgaggt cctcggcgtc cgactcgctg cgccactcgt ggtcgagcac gcggtcgcgc
    14701 cgcgcaaccg ggatcgacgc cgccggcacg ccgctgcggt actgcgccga gtagaggtcg
    14761 atgccgagcg gcagctcata gggcaggccg cggtagaccg tgacgaagcc cgtgtcgtcg
    14821 gtgccgagga agtagacctg ccgcgaggcg agatacgccc cgaccgcgag cacgccggtg
    14881 attgccaggg cagccactcc ggcgagcacg cgacgcgcct tggccgaccg gctggccggt
    14941 cgcttgcgtc ccgcgccggc acgctcgcgc ggcagcgacc ggctcgacgt cgcacccgcg
    15001 gtcgggggca gcgtcgccga cgaggccctg gacagatcgc ggtcgcgctc ctcggccgcg
    15061 gcgcgcacgt cgtccgcact gaggccctgg tggatcgtct cctccgcggt cggcgggtcg
    15121 ccggccgggc cgctgccgtc gtcctcgagc cggaacagca cgacggtgat gttgtccttg
    15181 ccgccgctct ggttggcggc gcgcacgagc gcctcggcgg ccgactcgag cgacggcgcg
    15241 gcgcgcacga gggtcgccag ctcgtcgtcg gagatcatcc cggtcaggcc gtcggagcag
    15301 agcacgtaga cgtcgccgga gcgcgccgcg tgggtgtgcg tgtcgacctc gacctcgcgc
    15361 tcgggtccaa gcgcgcgcgt gatgatcgag cgctgcggat ggtgctcggc ggcctcgggc
    15421 gtcagctggc ccgaccgctc gagctcggcg accagcgagt ggtcctgggt cagctgggtc
    15481 agggcaccgt cgcgcagcct gtaggcgcgg ctgtcgccga cgtggccgac gctgacgcag
    15541 tcgccgtcga cgagcgtcgc ggtcagcgtc gtgcccatcc cgcgccgcga ctcgtcgcgc
    15601 agcgccagct cgtagatccg ccggttggcc tcctgggcga cgcgctcgag ctgctgctcg
    15661 ggcgtttggc cggggtcgcg cgggtcgcgg aacgcctccg tggcgatctg cgaggcgacc
    15721 tctccggcgc gcgccccgcc catgccgtcg gcgaccgcga agaccggcgc gtcgagcacg
    15781 aggttgtcct cgttggcctc gcgctgacgg ccgacgtcgg tcagccccgc ctgttctacg
    15841 actcgcagcg ccatggctac tcctggtacc ggtactcgct gtcgccgatg cggatcacgt
    15901 cggcgacctt gagtcgctcg gccccgcgca gctgccgccc gttgaggtac gtaccgttgg
    15961 tcgagcccat gtcctcgacc agcatgaagt cgccgcgcgg gaagatccgc gcgtgggccg
    16021 acgaggcgaa cgggtcgtcg acccggatct cggcgtggtc ggagcggccc atcgtcgcgc
    16081 cgtcgctcag gtcgaagacc gtcccgggct cgtggccgag cgccgcgatc acctcgagtc
    16141 gcggctccat cgagccgagc atcccgccgt tgcgcgagtc gtcgcgcagg cggccaccgg
    16201 cctcgggggc tggctgcgcc gccccgccac cgaccggctc gacgccgcgc gagaggtcac
    16261 gcagcgcact gcgcgccacc cagagcagga agaggtacag caccagcagg aaaccgaact
    16321 tcaacgcgac cgcaaccggc tcgtccatcg tgctcctcct ctctactcga gctcgaagtc
    16381 gaggtcggtc aggcccaacg cgatccggtc gccgctgcgc agcagcgcct cgtcgacgcg
    16441 gcggcggttg accttgatgc cgttggtcga gccgaggtcg gcgatccgcc acgagtcgcc
    16501 ctcggcgcgc agctcgcagt ggtggcgcga gatgttcggg tcgtcgatca ccatgtcgca
    16561 gtcgcggctc cggccgatcg tgaagcgttc cccctcgatc acggtgcggc ggccgtcccc
    16621 ggccagtacg gcgcggcggc cggtgccacg gggcggccgc tccggcgcca gggcgcgcgc
    16681 ccctgcacgg tcgggcgagt agaccatcgt gtggccgaag tcgccctgcg agggcgcgac
    16741 cgcagccgac gccggctcgt gctcctcctc gggcgggctc agcagctggg cctggatgcc
    16801 gaactcgccg agcgccaggc gctcgtcggt catgaactcg acgaccgggc ggctggtcag
    16861 cgcgaggctc tcggcgcgtg cgtgctcgag caggtagtcc gagagctcct tgcgcagcgc
    16921 cggctcgtag gtcgcgaact gggaccgatc gtccggcgac aggaagacgc ggtagtggtt
    16981 cgggacgtag gtccgcgaga ccgaggccat cttcgactcc tccatctcct tcgccagctt
    17041 gcgcgccagc tcgaccggct cgacgctcga cttgaaggcg cgactgaacg ccccctcgac
    17101 gaggccgccc agcttcgact cgaggtttcg cagcaggctc accgaaatcg acccatcagg
    17161 gtagtgccgg tccccgccgc acgcgcgcca gcagcggcag cgcaaccgcc gctccgaccc
    17221 aggcgatcgc caacaccggg tcgaccgacg tgaccaccgg ctcgaccgcc tcgcgcgccg
    17281 cgtcgaccac tgagccctcc cagccggcgc gcggcggcgt cgccggcgcg ctgccgtaga
    17341 gcaggtcggc accggtcagc gcctcggtga agcgcagcca cagcaggccg gccgcggcca
    17401 gaccggcacg gcgccagacg gtcccgccga gccacgccgc agctaggacg aacagcggcg
    17461 ccagtccaat cgcaccgagc agtggcgcca acgcccccgc cgaccagagc cgaccggcgc
    17521 gcggcagcag cagcgggacc ggcagcagcg cggcggtcag cacgagcgcg gtgccctcct
    17581 ggccgccctc cggcgaagca agccagccgc agaccgcgag cgccgccgcg atccagccga
    17641 ttcgcggcag cagcgcgacc gcgccggccg cgacgagcgc gacggtgccg acggcgaacg
    17701 acgggtccgg acccaggccg atcccgagtg tgacgaggat cgcggcggcg gccccggagg
    17761 cgacgcggcc cacgattgcg tcgcgccacc cctcacgcgt cgcgtgctcc tcctcacccg
    17821 ggacgacgat cggcggtggt cggcgggacc ggcgcagcac gcgcgtcagc cgcgtccgcg
    17881 ggcggacagc ggtcagcccg aagcgctcga tgacctccgg ctcgaccagg ccgccctcgt
    17941 ccggcagctc ctgctcggtg tcgaccagga cctcgcgcag ctcggcgagc gtcggccggt
    18001 agcccgggtc aggatcgagc gcggcgtcga cgacctcgca gagcgccgcc ggtaggtcgc
    18061 gccggcggcg gcgcagcggt ggcagcggcg cgccgacctg gcgcgcggtc gccgccgggc
    18121 tggctccccg gaccgggttc gagccggtcc aggcctcgta gagagtcaac gcgagcgagt
    18181 agacgtcgcc ctccgggccc accgcgccgc cctcggcctg ctcgggtgcc atgtaggcga
    18241 gcgtgccgac gatgtcgccg gtggcggtca gcgcgtcgcc ggtcgcgagc cgggcgatgc
    18301 cgaagtcggt cagcttcgcg aaccccgcgc cggcggccgg ctcggcgagg accatcacgt
    18361 tgccgggctt gacgtcgcgg tggaccacgc cgcgcgcgtg ggcgtgctcg agcgcctcgc
    18421 acagcgccgc gccgatcttc gccacgtcgc ggtccgagac cgcgccctcg cgcgtcaggt
    18481 cggccagcgt cgcgccctcg accagctctg agacgaggta agccgcgtgc tcgtcgtgac
    18541 cgagctcata gagcgcgacg atgcccgggt ggttgagccg cgccgcggcg accgcctcac
    18601 gctggatccg cgcctcgtcg cccgcgtcgc caccgaacgg gaccaccttg acggcgacgt
    18661 cgcgctccag ccgctcgtcg tgcgcgcgcc agacgacgcc gaacccgccg gcgccgagac
    18721 gctcgccgag tcggtagcgg ccgagcacga cacggccgga aggcggccca ccgaggcgct
    18781 cggtgcgcgc gtcgtcgagg cgctcggtgt gagcgtcgcc cgcgatccgc cgtgtggtct
    18841 gagacctgct catctgggtt cggtgttcgg tccccgggcg gacctacctg ccatcgcgac
    18901 cgggggctgc aggaaggaca accgatggaa aggtcatact ccccctactg cagggcctca
    18961 cgcgaggaaa cgattcagtc cttgttcgac gaaccggacc agccaacagc cggccgcggt
    19021 cgcccggccc gccaacggcc ccgaccaggg ccctcgaccg accgtcagac cttgatggta
    19081 aggcgatcgc tcgcgctcgg cgccgggctg ctcgtcctga tcctgctgat cttcggcgtc
    19141 cgcagctgca tggacaacca gaaagagagc gcgatcaccg actgggtgcg tgacgtcgac
    19201 gcgctgatga agcagtccaa cggcgagagc gaggccctct tcgaccagct cggaggcggc
    19261 ggcgggaccg acgtcgatgt cgagaacgcg ctcaacagct tccggatcca gtcggcgcag
    19321 ctcgtcgacc aggccaacgg cctcgaccgc cccggtgagc tcgaccgcgc ccaggaggag
    19381 ctcgtcgaga cgctcacctt ccgcgccgag gggatcgccc agatcgccga cgccctgccg
    19441 aacgcgctga ccgacggcga gcaggccgag ggtgccgcgc gacggatcgc ggaggcgatg
    19501 cagctgttcg tgacgagcga tcagatctac ttcaagcgcg tcgtgccggc gctcgacgag
    19561 gtgctcgagc aggagggcct gctgctctcc ctgaccaaga gcaccttcct gcccgacctg
    19621 tcctggctcg acccggccga ggtcgccgac cgcctcagcg ggatcgcgag cggcggcggc
    19681 gaggagggcg acgaggaggc ggcgcccggc ctgcacggca acggcctcgg cactgtcacg
    19741 ctcggcggcc agacgctggt cgcgggcgga tcgaccagcg tgacggcgac cggcgacctc
    19801 tcgttcgagg tccaggtgat caaccagggc gagaacaccg agaccgacgt caccgtgacc
    19861 gcgaccgtcg gcagcggggc cgacgagatc gtcgccgagg gcgtgatcga cacgatcgcg
    19921 gccggcgaga gccaggtcgt cacgatccgg ctcgaggaga cgccgccgac cggtcaggcc
    19981 gtcccgatca cgatcgagat cgagctcgtc ccaggcgagg acgcggatat cggcaacaac
    20041 agcggcgagt tctccgtgat tttcacctcg tagccccggg taggctgcga gcgatggacg
    20101 gcctgacgac gaccgagggt gtggtggcgc tcgccggcgc aggccttgcg ctgatcgcgt
    20161 tgctgctggt gctcgtcttg gcgctccggc tgcggcggct gcgcgccgat caacgggccg
    20221 tgctcggggc ggggggtcag cgcgacctcg tggagcacgc ggcgcggctg gagcgcgggt
    20281 tcgtcgagct gcgcgactgg gtcgaggaca cggccgcggg gatcgagacg cgcatgggcg
    20341 ccgccgaggc gcggatcgac ggctgcgtcg cccaccgggc gctcgtccgc tacgacgcct
    20401 acggcgagat gtcggggcag cagtcgagct cgcttgcgct gctcgacgcg cgccgctcgg
    20461 gggtggtgat ctcgtcgatc caccaccgcg acagcgctcg ggtctacttc aagcaggtcg
    20521 tcgcgggcga gtcggagctc gagctgtcgc ccgaggaggc cgaggcggtc gagctggccc
    20581 tggcgggcgc cggccaggcg gcgcagtgag cgacgctccg cggcgagtcg cctacctggg
    20641 accgcccggc acgaacaccg acgaggcgct gcgcgcgtcc gcgaccgagc cgttcgaggc
    20701 ggtcgcctgc acgaccgtgc ccgacgcgat catggcggtc caggagggca cggtcgatcg
    20761 cgcggtcgtc ccgatcgaga actcgctcga gggcggcgtc gcggcgacgc tcgacgcgct
    20821 cgccggcgag gcggccgatg tgcggatcgt gcgcgagacc gtgcacccga tccaccacca
    20881 cctgctcgcc gcgggtccgc tcgagctcgg cgccgtccgc agggtgcttt cgcatcccca
    20941 ggcgctcggc cagtgctcgc gcttcctgcg cgagcaactg ccgcaggcgg agatcgccgc
    21001 ggccagctcg acggccgaag ccgtccgctc actgtccgag gccgacgagc cgtgggctgc
    21061 gatcgggtcg cggctcgcgg gcgagctcta cggcgcgacg atcgtcgccg agcggatcga
    21121 ggaccgctcc gacaacgtga cccgcttcgt ctggctggcg ccggccgacg cggcggcgac
    21181 gccggcagac gggcccgcga agacctcgat cgtcttctgg ggcttcaacg acgcggcgcc
    21241 cggcgccttg gtgtcggtcc tgcgcgagct ctcggaccgc gagatcaacc tgacgaagat
    21301 cgagtcgcgc ccgcgccggg tacggctcgg ccactacatg ttcttcgccg acctcgaggc
    21361 cagcgccgcc gagccgcgcg tggaggaggc gctcgcagcg ctcggcgaac gcgtcgagac
    21421 ccttcggatc ctcgggtcgt atcccgccag cccgcactag acccccgcta cactggccgc
    21481 accgtgacgg cccatgcggt accaccagcc gagtctcaag ctggcgacag cgacccagcg
    21541 cctatcggcg cgccgccgga cggccggtcc ggcgggcgcg cttcctcatc cggccgcgtg
    21601 ctcgtgctca acgcctccta cgagccgatc aacgtctgca ccgtccgccg cgcagcggtg
    21661 ctgatcctca aggaccgcgc cgagatactc gagcgcagcg actgggcgct gcacgccgag
    21721 agcttcacgc tgccacgccc gatggtgatc cgcctcaaga cctacgtccg cgtgccgcgc
    21781 gacgcccacc ggcgcaagat caccaggcgc gccgtcttcg cccgcgacca gtggacctgc
    21841 cagtactgcg gcgccgagcg cggcagcctg accgtcgacc acgtgatccc gcgctcgaag
    21901 ggcggaccga ccgactggca caacgtcgtc acgtgctgcg cgccctgcaa ccggcgcaag
    21961 ggcgaccgcc tcccacggca ggcgaacatg gttccgcgcc atcccccgtc cgcgccgaac
    22021 tctacgatct tcatacacgt cgccgtaccg gtcatcccgc agtcgtggga gcggtacata
    22081 ccaagcgccg cctgacccct tggagtccag caacccccca aagccgcccg gtcagccagc
    22141 cgggtcgagc ccacccccga ctccgccgag cgagcagccg accaagccga tcccgcccgc
    22201 cgacgctgcc gcggcgcgcc ggaccgacgc cgccgtcgtc gagcacgccg tcgaggaggc
    22261 gatcgagcac gacggcctca ggaccgtccg ccgctggatg atcgtgctca gcatctgggc
    22321 gctcgccgcc accgcgatcg cggtgatcgc gttcatggag gcgcgcgacg cgaagaccga
    22381 ggccgactcc gcgcagtccg cgacccaggc caacatcgag cgtgtccagc agcgcctccg
    22441 cgatgacgtc gaccagctcc gcaccgacgt cgaccgcctt ccgacccagg ccgacgtcga
    22501 gcagctccag acccgcgttc gcaaggcgca acgcgacgcg acggccgccc gcgacgacgc
    22561 caccgcagcc acctccgacg ccgcgggcct ccgccctgac atcgaggagc tcgagcgcca
    22621 ggtcaactcg ctccgcggcg tcgtcaacgc ccgcggcgac tagccggtcc taccgacggg
    22681 ctctcgcaac gacgaggggc gccccttcca ggacgcccct cgctactgcg cttgactgga
    22741 ccagctcggc gacggactcg acgaccggcg ggacctaacc cggcctccgt ctccgagggt
    22801 gccacggttg gactccactc gacggggtcc agacatccgg tctagtggcc ggacacctcc
    22861 agggtcccga aacgtctgtc actcaacttt ggaccacctc ctttccctag tccgcccatt
    22921 gaagcagacg cccgcgaccg accacgccgg ttcggttcgc ggccctccgg caccggccta
    22981 ctcggcgtcg ggctggtcgt cctcggcgtc gttctcggtc ggcgcgacgt caccgctgat
    23041 gtcggactcg tcgggctcgg cgacgccggc cgtctcgggg tcgtcgggtg aggtgagctc
    23101 ggcgatcggc tcgaggccgt cgggggcgcc gtcgacgacc gcggcggtgt cggcctcgtc
    23161 ctccatgacg agcgcgaccg cgctgacgat gtcgtcctcg cgcaggttca tcacgcgcac
    23221 gccttgggcg ggacggccct gctgggagat cccgcggacg ccggtccgct ggaccatccc
    23281 gttctgggag atgaagacga ggtcctggtg ctcgcggacg atcagcgcgc cggcgacgcc
    23341 gcccttcttc tcggtgatgt tgatcgtctt gacgcccatc gcgccgcggc ccttgacggg
    23401 gtactcggcg atcggcgtgc gcttgccgta gccgttctcg gtcacgacga gcagctcggt
    23461 gtcgtcgcgc gcgatgtcca tcgcgagcac gcagttgtcc ccgcgcgaga cgttcatgcc
    23521 gcgcacgccg ccggtgtcgc ggcccatcgg ccgtgcctgc tcctcgttga agcgggcggc
    23581 ctggccggag cgcgagatca tgatgatgtc gtcgtcgccc gaggtccgcc gcacggcgac
    23641 cagctcgtcg tcgtcgcgaa tcttgatcgc gatgattccg tcggccttga tcggcgtgtt
    23701 gtaggcgagg aactcggtct tcttgatctg gccctgcttg gtcgcgaaga cgaggtactt
    23761 gccctcgcgg aagtcgcgcg tcgagaggac cgcgcgcacg aactcgccct cgcgcagcgg
    23821 cagcacgttg accagcgcgc ggcccttcgc ggtccgcgac gcctccggca gctcgtagac
    23881 cttctggcgg tagaccttgc cgcggttggt gaagaagagc agatagtcgt gcgtcgagca
    23941 gacgaagagg tgctcgatgt agtcctcgtc cttcatgtcc atgccgcgca cgccgacgcc
    24001 gccgcggtgc tgcttgcgat aggtcgcgag cggca
//
LOCUS       DATABV010000196        16523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_63375, whole genome shotgun sequence.
ACCESSION   DATABV010000196 DATABV010000000
VERSION     DATABV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16523
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63375"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..365)
                     /locus_tag="VHF88_08930"
     CDS             complement(45..365)
                     /locus_tag="VHF88_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161929.1"
                     /translation="MPLTDRPEFRAWLDRHNQRSVGRPHGQPKGCLTCGTALDHELST
                     QECLVCLAERFGIETAERWIADVLAGLVRAAADPLVLALVLAEIVPFARDVIEHRVPP
                     PAGA"
     gene            complement(566..1747)
                     /locus_tag="VHF88_08935"
     CDS             complement(566..1747)
                     /locus_tag="VHF88_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2161930.1"
                     /translation="MRMGISRPVSGHVFRVDRKRGPVWFAKYRLPDGRQVQKKLGPAW
                     TRRGRPEAGHFTKALAQEWLDSTLAEARRGELAGMVRTGATFAEAAAEWLVYVERQRA
                     CKPRTVADYSMMVRILNGSFGDRRLEEFTPEGFEAWKVYFARERELSNRTLQKYLVVL
                     HAIFKRAMKVHGLPRNPLAVVERPRLPRRAGIDVFSRDEVMALVAAAANHQDGVLYLT
                     AAFTGLRRGELLGLRWEDVDVGLATIRVRRSWSSGLEGTPKSGRGRAVPLMPEVGEAL
                     DGLRKRDRFTGDRDLVFCDPLGRHLGEKSFGVRYKASLRAAGLRELRFHDLRHTFGTH
                     AIRAGDPREVMEWMGHADIQTTQKYLAYKPRRDAAKRLAAAFDEAAPSDASDLPTVTA
                     Q"
     gene            complement(1847..1920)
                     /locus_tag="VHF88_08940"
     tRNA            complement(1847..1920)
                     /locus_tag="VHF88_08940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1884..1886),aa:Arg,seq:tct)
     gene            1992..2285
                     /locus_tag="VHF88_08945"
     CDS             1992..2285
                     /locus_tag="VHF88_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2161931.1"
                     /translation="MKLYTCGQKGKGGSLPWPVTHACGAAMKALDAAGYDYELQAVPG
                     YRLLPWTRRGEARAEIRRLTGQDNVPVLLLDDGTAIAGSGKIVEWARAQPKTV"
     gene            complement(2323..2396)
                     /locus_tag="VHF88_08950"
     tRNA            complement(2323..2396)
                     /locus_tag="VHF88_08950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2360..2362),aa:Pro,seq:tgg)
     gene            2619..3890
                     /locus_tag="VHF88_08955"
     CDS             2619..3890
                     /locus_tag="VHF88_08955"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX2161932.1"
                     /translation="MAEVQPLKTLRYDESVVGPLDGVIAPPYDVIDATLRAELVAKSP
                     YNVVEIDLPVEGSEDDRYLHAETLLDHWRTQGALVQEDEPAFWALDQEYTAPDGSKQR
                     RRGFFARVRVEDYGAGRIRPHERTHPGPKEDRLRLTRATRANLSPIFSLFPDPSGDAA
                     EVLAEAVAETPFASATDHEGTVNTLWRIAHPDRVAALQAALADAELLIADGHHRYETA
                     RVYADEIGGEGDHRYVLMFLCSLSDRGLLVFPTHRLLSGLKDAPAKQEAIRDTLKRDF
                     EIEPVERDRLEPAGDAQGRVQLGYMDSFHRQPFLLTLKDQAIADAALAGMPEPYRRLD
                     TAVLEAIVLKGALGMSEDDISHLNGLDYSKSFDDALDAVESGRCDAGFFMRGTPAEQV
                     SEVAEAGVSMPPKSTYFYPKIPTGLIFNLLQ"
     gene            3895..4455
                     /locus_tag="VHF88_08960"
     CDS             3895..4455
                     /locus_tag="VHF88_08960"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX2161933.1"
                     /translation="MKIYTRKGDDGTTGLWYGGRVGKASPRPEAYGSIDEAASALGVA
                     RAITEPGSELHDDVLRFQNELFVAGAELATAPSAADRLQDGVSRVTEEMVDQLEQDID
                     RYMDRVDLPPKFVIPGGNELSARLDVARAAIRRAERRVSALHDAGELADDTVLRYLNR
                     LADAVFALARFADRPDPELFQGRDAG"
     gene            4452..4853
                     /locus_tag="VHF88_08965"
     CDS             4452..4853
                     /locus_tag="VHF88_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2161934.1"
                     /translation="MKHAVARRVAGDGLKHHIEVGGHELTSDEPERLGGTDAGPSPEE
                     LLAASLASCSAITLEMYARRKGWDVGDVVVEVDYEPAQRGSPTRFAMSVRLPKELPEE
                     QRERLMQIVAKCPVHRTLEGEVMFDEKLELF"
     gene            complement(4850..5608)
                     /locus_tag="VHF88_08970"
     CDS             complement(4850..5608)
                     /locus_tag="VHF88_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2161935.1"
                     /translation="MVSNGSSPQELTIDELGRETGMTVRTIRSHQARGLLPPPEVRDR
                     VGYYGPEHVARLKLIQELQAEGFNLRGIKRLLATTGGPAQQLLGFGEVVRAPFAAEPS
                     QVFTRDELLERFGGAGGEEMLAQAERHGALVALGGDRYEAPVPSLLDLAEEVVSNGVS
                     LDAAIAVMAKVNERCESIARTFVQLYLTEVWKPFTEAGYPEERWPEVMRSIEGLRPVA
                     AQAVQAMFGLTMTREVEAAFGKEVGRLAKSKGAS"
     gene            5668..7515
                     /locus_tag="VHF88_08975"
     CDS             5668..7515
                     /locus_tag="VHF88_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161936.1"
                     /translation="MIRDLLYAGAASPPAGGAATGEIALATVSATLLTLGLLYLGDGH
                     RRGTVSVLGKVAAFATRVSGQPGWAAFPAALATLSLLTAVIGMYWDIALHVNVGRDEG
                     PLANPAHYFILAGLYGIFAAGFFAMVLPKEKPSASSVRLGKDWHAPLGGVLICACGAF
                     ALLGFPLDDVWHRLFGQDVTLWGPTHLMLIGGASMTLVGIAVLQTESRRSMAAAGKTL
                     DEAPWLHKLRLVALTGGLLLGMSTFQAEFDFGIGQFRLIFQPMLIMLAAGVVLVAARI
                     WLGRGAALGAVLFFILVRGFLAVLVGPILGETTPHFPLYIASALVVELVALAIAADRR
                     PLAFGLWAGVLIGTVGLAAEWGFSQLWMPLPWTAALFPEGALLGFATAVCAALIGAWV
                     GAHLASETIPRTPSLRAAGAIAAAGVGAMVLWALYTPPGPPTTVAVTTRDIDRGAERT
                     IAATVRFDPPNAAEDAEWLTATAWQGDGLVVDRLERVREGVYRTTEPIPVHGNWKSMI
                     RLQRGNAIDAAPIFLPDDPAIPAPEVPAPAQFTREFVPDSEVLQREQKTDVAGTLTAI
                     AYSVVAAIALSLLALLAWGLHRLALTSGTPPRSRASRKADRPAILAARR"
     gene            7512..9041
                     /locus_tag="VHF88_08980"
     CDS             7512..9041
                     /locus_tag="VHF88_08980"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2161937.1"
                     /translation="MTSASVPAPSAVPDVDHVDVLIVGAGLSGIGAAYHLQTKLPGKS
                     YAILEGRDAIGGTWDLFRYPGIRSDSDLHTLGYAFKPWTDEKAIADGPAILAYIRQTA
                     RENGIDRRIRFGHRVVRAEWSSADARWTVEATRSDDGATVAMTCDFLFAAGGYYRYDE
                     GFTPHFEGRERFQGQIVHPQQWPEDLDYAGKRVVVIGSGATAVTLVPAMAEEAAHVTM
                     LQRSPTYIVSVPSRDPIANALRRVLPNRVAYSVARRKNVALQALVYKLSRRRPGIVRR
                     LIRAAVKRQLPDGYAVDTHFKPHYDPWDQRLCVVPDGDLFKAISSARASIVTDRIASF
                     TERGVALASGTELEADIVVTATGLNLVAFGGVEYVVDGEPVVLADTFAYRGAMLSGVP
                     NFAFAVGYINASWTLKVDLVCDWLCRLLAHMDSHGYAQVVPVNDDTDLPRRPLLDFSA
                     GYVQRALDSFPKQGPAAPWRMSMSYREDVRNLRDSELVDGTLRFTRRPAVERQPDGVA
                     A"
     gene            complement(9038..9808)
                     /locus_tag="VHF88_08985"
     CDS             complement(9038..9808)
                     /locus_tag="VHF88_08985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2161938.1"
                     /translation="MAQIDDAEGWRRLIASLAQWSRTIDGASDGMRVAERDGLVTTIT
                     PRVPDRSIVNDVVYWDRDALRDAYDEIEAAYAAAGIAAWTVWVPDSDEQTAALLADRG
                     HVLDADPEAMVLDLDSIERPPAPTGFTRELDPIELARLNDDAYGMRDAFAKALDRLEA
                     RDGLYLYGVEEDGELASGLVTFDHGDDCSVWLVATRELSRGRGLAGALMGQAMADARE
                     RGRTTSTLQATDLGRPVYERLGYRSLGEIQMWEKRAGG"
     gene            9827..10393
                     /locus_tag="VHF88_08990"
     CDS             9827..10393
                     /locus_tag="VHF88_08990"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX2161939.1"
                     /translation="MPTAAELRELLLTPEQAAAMDAPGRTDAAVLVPIFHRDGDPVAV
                     FTERRADLRRHAGEISFPGGRQDFPDEDLRTTALREAEEEIGLERDDVELVGALPPVG
                     TFVTGYRVFPFVGLIEPGRAWVPQETEVAQVLELSLADLVRGYESKRLTRRGVPIKTP
                     TYTVDGHLVWGATARIVQHLLRRLEPLL"
     gene            complement(10390..11229)
                     /locus_tag="VHF88_08995"
     CDS             complement(10390..11229)
                     /locus_tag="VHF88_08995"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX2161940.1"
                     /translation="MAEYAKDVLVETQWVEDHLDDDSIRIVEVDENPALYAEAHIPGA
                     IGFDWKTDLQDQVKRDFLGPQAFGELFGSRGISNDHTIVLYGDRNNWFAAYTYWYLKY
                     YGHDNVKLMNGPREKWISEGRPVTSDAPSYPAASFTAKPGDDAIRAKRDEVLAALGKG
                     TNLVDVRSPQEYSGELIAMAGYEQEGAQRGGHIPGAASVPWAQAVREDGTFKSADDLR
                     ELYGGKGVLDGETIAYCRIGERSAHTWFVLHELLGQQDVKNYDGSWTEWGNLVDVPVE
                     KDV"
     gene            11745..12674
                     /locus_tag="VHF88_09000"
     CDS             11745..12674
                     /locus_tag="VHF88_09000"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX2161941.1"
                     /translation="MRTPSGIAYAIAVRDAVDAAAPVDPPPNRLDPRDALPLLADALR
                     ASAPEGVERPSVALLSDGPSNSAWYEHRALAAALDIPIVREDDLAVHDGRLHMRRDGA
                     RTQPVDVIYRRTDEDRLRGADGEPTWLAGKLLEPLRAGTLAVVNPFGAAVADDKLVHA
                     YVDDMIRFYCGEEPLLKSVPSYDLGNPAIFDDVFERLDELVVKPRSGHGGEGVVVCPR
                     VSPQARDAVRRAVAADPDGYIAQEPVDLSTEPTVCDGRLEPRHVDLRPFAIAVGDAVV
                     TAPVALTRVALERGALIVNSSRNGGGKDTWLLS"
     gene            12659..13450
                     /locus_tag="VHF88_09005"
     CDS             12659..13450
                     /locus_tag="VHF88_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2161942.1"
                     /translation="MAAELTASRPLIGVTTSEVRFAARIQQTAQSEPPRREMALGLTY
                     LLAIEAAGGLPVVMPPLELEAIEPLLDRLSGICLSGGPDLDPTAYSEQPHPHLGPTEP
                     DLDRFELELARRADTRGLPILAICRGMQALNVARGGSLHQHLPDRPGVAIDHRQREGG
                     DHAIHDVTIAPGSRLARLMRRDAAEVNSFHHQAIHRIGSGLRAVAWSPDGVIEGIEAP
                     ARDFVVGVQWHAESLVELREQARLFRGLVEAANRFEGRDAGARAA"
     gene            13565..14623
                     /locus_tag="VHF88_09010"
     CDS             13565..14623
                     /locus_tag="VHF88_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX2161943.1"
                     /translation="MACCRSSLRRVEDHVTRETHTSALELSTGVHATAAALREELISL
                     RTRLGRELSLLGLRAASAGTHPSTVWHETVVSTGPRYEAVYGSMRELARREPTFGLHV
                     HVAVRGPAEGIRLFNRMRVHLPLLLALSVNSPFWQGRDTRLASARTPLFQAFPRVGIP
                     RAFRDYEDWVGAVDLLIRCDAFPDPTFLWWDVRPQPRFGTVEVRVLDAQTTASESAAL
                     AALVQCIARLEVEEGYVATELIGAQEALEENRFIAARDGMDARLIDPLDECRTPVTEH
                     LDRLLEASAPHAAALGCERELELVEAMARQTGAKRQLALARDEGRLPGLVEALADRFL
                     ADLEPPAAEGGAPAATAA"
     gene            complement(14624..14920)
                     /locus_tag="VHF88_09015"
     CDS             complement(14624..14920)
                     /locus_tag="VHF88_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161944.1"
                     /translation="MDEFAWLVTGSLIAGVLILLAIGKWYPGSGAEQVDWKPTRSVEL
                     EIELELDDVDQMLEANNERRRASGRPELTEDGLREELAAEERLRPRGPSDGPPF"
     gene            14974..16131
                     /locus_tag="VHF88_09020"
     CDS             14974..16131
                     /locus_tag="VHF88_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2161945.1"
                     /translation="MIDFELTDEQRLVRDTAREFADNEIVPRARDNDRSGRFDTELVG
                     KLADIGFIGPILPEEYGGRGIDYRTYGLIVEEIGRADSSARTVVSVNTSLVGSAIAKW
                     GTEEQKQSFIPGICSGEALGCFGLTEPDTGSDAANLSTRAERVDGGWRITGQKQWISL
                     GNHAKFALIFAQTDPEKAHRGLGAFLVPTDSPGFSSAEIHGKLGLHASDTAELALDGV
                     EVGDDALLGEVGDGFKVAMSALDSGRFSVSAGCVGICQGCVDASTSYAKERVQFGRPI
                     AAFQLVQAMIADMVVDTHAARSLVWKAAWLKDTGQPNTLETSVAKLFSTEAAIRSANT
                     AIQVHGGSGYVDDHPVERYLRDARVTTLYEGTSQIQKLIIGRLTTGINAMT"
     gene            complement(16115..>16523)
                     /locus_tag="VHF88_09025"
     CDS             complement(16115..>16523)
                     /locus_tag="VHF88_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161946.1"
                     /translation="RGLLAALRDEDAEAGVRAGVAHHLGWVEANRARARFLMRRREAE
                     VVAVSKGDVAVLNKRFFAALAEWHAPLAADGAIRDLEIPLLCAIWWGPAQEYARGVLE
                     SGRSPRSLLADAEPALADAAWAALAPPRLRSSR"
BASE COUNT         2368 a         6087 c         5662 g         2406 t
ORIGIN      
        1 gcggggccgc gggcgggagg aacccgcggc cccgcgatag cgacttaggc gccggcaggc
       61 ggcgggacgc ggtgctcgat gacgtcgcga gcgaacggga cgatctcggc caacaccaat
      121 gcgagaacga gcggatccgc cgccgcgcgg accaggccgg cgaggacgtc ggcgatccac
      181 cgctcggccg tctcgatgcc gaaccgctcg gcaaggcaga ccaagcactc ctgggtgctg
      241 agctcgtggt cgagggcggt gccgcaggtc aggcagccct tgggctggcc gtgtgggcgg
      301 ccgacgctgc gctggttgtg gcggtcaagc caagcccgaa actccgggcg atcagtcagg
      361 ggcatctcgg tacctccatc tgttggaggc gaccggacca ggacagcgac aaacaccgcc
      421 acggcgatac cgtgcgggcg cgtctgagtc tcggctcggg cgccacgccc ccggcctgtg
      481 caaccaggcg cgggggcact tatctatcga acgccgccga acctagacgc cgcctgcaga
      541 tatggcaagg gcttcgccgc cgtcgtcact gcgctgtaac ggtcggcaga tccgaagcgt
      601 cgctaggtgc ggcctcgtca aacgcggcag cgaggcgctt ggccgcatca cgacgcggct
      661 tgtaagcgag gtacttctgc gtcgtctgaa tatccgcgtg gcccatccac tccatgacct
      721 cgcgcggatc gccggcgcgg atcgcgtgag tgccgaaggt gtggcgtaga tcgtggaagc
      781 gaagctcgcg caagccagcc gcgcgcagcg atgccttgta gcggacgccg aacgacttct
      841 cgcccaggtg acggccgagc ggatcgcaga agacgagatc gcggtcgccg gtgaaccggt
      901 cgcgcttgcg tagcccatcg agcgcttcgc cgacctcggg catcagcggc accgccctcc
      961 ctcgcccgct cttcggcgtc ccctccaagc cgctgctcca gctgcgtcgg acacggatcg
     1021 tcgcgagacc cacgtcgacg tcctcccaac ggaggccaag cagctcgccg cgtcgcaggc
     1081 cggtgaaggc tgcggtcaga tagagcacgc cgtcctggtg gttcgcggcc gcggcgacca
     1141 gcgccatgac ctcgtcgcgt gagaagacgt cgatcccggc gcgacgcggc aaccgcgggc
     1201 gctcgacgac cgccaacgga ttgcgcggca ggccgtggac cttcatcgcc cgcttgaaga
     1261 tcgcgtgcag aaccacgagg tacttctgca gcgtccggtt cgacagctcg cgctcgcggg
     1321 cgaagtagac cttccacgcc tcgaagccct ccggtgtgaa ctcctcgagc cggcggtcgc
     1381 cgaacgaccc gttgaggatg cggaccatca tcgagtagtc agcgaccgtc ctcggcttgc
     1441 aggcgcgctg ccgctcgaca tacaccagcc actcggcggc cgcctcggcg aaggtcgcgc
     1501 ccgtgcgcac catcccggcc agctcgccac gacgcgcctc ggccagcgtc gagtcgagcc
     1561 actcctgcgc cagcgccttg gtgaagtggc ccgcctccgg ccgaccgcgc cgcgtccacg
     1621 ccgggcccag cttcttctgg acctggcggc cgtcaggcag gcggtacttc gcgaaccaga
     1681 ccggtccacg cttgcggtcg acccggaaga cgtggccgct gaccggccgg ctgatcccca
     1741 tcctcatccc ctcaacctcg gggatctcgg gacatattgg ggacacggtt tgcatcgagg
     1801 ccgttacagc cgacgcacaa ccctgtgttt tgcaggcgct ttcgagcgcc cccggctgga
     1861 ttcgaaccag cgacctgcgg attagaagtc cgctgctcta tccagctgag ctacgggggc
     1921 ctggttgaat gatcgcctct acgcgggatg ttgtcgaacg atgtcccgct acccacgagg
     1981 gagacgctga gatgaagctc tacacctgcg gacagaaggg caagggcggc agcctgccct
     2041 ggcccgtcac ccacgcgtgc ggcgcggcga tgaaggcgct cgacgcggct ggctatgact
     2101 acgagttgca ggcggtcccc ggctaccgcc tgctcccctg gacccgccgc ggcgaggccc
     2161 gtgccgagat ccgccggctg accggccagg acaacgtccc tgtcctgctg ctcgacgacg
     2221 gcaccgcaat cgccggctcg ggcaaaattg tcgagtgggc gcgggcgcag ccgaagaccg
     2281 tgtagcgcgt accgcaccgc gcaaaaggcg cgctccctcc aatcgggacg actggattcg
     2341 aaccagcgac cccctgctcc caaagcaggt gcgctaccag gctgcgccac gtcccgaaga
     2401 cgtcgagtgt agggctcatg cggttcgtac acaacgctcc gctggctcag gcggctgagg
     2461 gattcgcatc gcccggcggt gccaatcgat cattgggcct ggccgggcag caggcatacg
     2521 atgccgctga gggaattaca tcgtggaggc ggtcgaatcc ctcatcggcg tcctcgccga
     2581 ggaccgcgat tggactctgc cgatagggtg ccgccgcgat ggcagaggtg cagccgctga
     2641 agacgcttcg ctacgacgag tcggtcgtgg gcccgctcga cggcgtgatc gccccgccgt
     2701 acgacgtgat cgacgcgacg ctgagggcgg agctggtggc gaagtcgccc tacaacgtcg
     2761 tcgagatcga cctgccggtc gagggcagcg aggacgaccg ctacctgcac gccgagaccc
     2821 tgctcgacca ctggcgcacg cagggcgcgc tggtccagga ggacgagccg gcgttctggg
     2881 cgctcgacca ggagtacacc gctcccgacg gcagcaagca gcggcggcgc gggttcttcg
     2941 cccgcgtgcg cgtcgaggac tacggcgccg gccggatccg cccgcacgag cgcacccacc
     3001 ccgggccgaa ggaggaccgc ctgcggctga cgcgcgcgac gcgggcgaac ctctccccta
     3061 tcttcagcct cttccccgac ccgagcggcg acgcggcgga ggtgctcgcg gaggccgtcg
     3121 cagagacccc gttcgcctcg gcgaccgacc acgaggggac cgtcaacacc ctctggcgga
     3181 tcgcccaccc cgatcgcgtc gcggcgctcc aggcggcgct cgccgacgcc gagctgctga
     3241 tcgccgacgg ccaccaccgc tacgagaccg cgcgcgtcta cgccgacgag atcggcggcg
     3301 agggcgacca ccgctacgtc ctgatgttcc tctgctcgct ctcggaccgc ggcctgctgg
     3361 tcttccccac ccaccgcctg ctcagcggcc tcaaggacgc cccggccaag caggaggcga
     3421 tccgcgacac gctgaagcgc gacttcgaga tcgagcccgt cgagcgcgac cggctcgagc
     3481 ctgccggcga cgcgcagggc cgcgtccagc tcggctacat ggactccttc catcgccagc
     3541 cgttcctgct cacgctcaag gaccaggcga tcgccgacgc cgcgctcgcc ggcatgccgg
     3601 agccctaccg ccggctcgac accgcggtgc tcgaggcgat cgtcctcaag ggcgcgctcg
     3661 gcatgagcga ggacgacatc tcgcacctca acggcctgga ctactcgaag agcttcgacg
     3721 acgcgctcga cgccgtcgag agcggccgct gcgacgcggg cttcttcatg cgcggcacgc
     3781 cggccgagca ggtcagcgag gtcgccgagg ccggcgtctc gatgccgccg aagtcgacct
     3841 acttctaccc gaagatcccc acgggcctga tcttcaacct gctgcaatag ccctgtgaag
     3901 atctacacgc gcaagggcga cgacgggacg accggcctct ggtacggagg ccgtgtcggc
     3961 aaggcctcgc cgcgtcccga ggcctacggc tcgatcgacg aggcggcctc ggcactcggc
     4021 gtcgcgcgcg ccatcaccga gcccgggtcc gagctgcacg acgacgtgct gcgcttccag
     4081 aacgagctct tcgtcgccgg cgccgagctg gcgaccgcgc cgagcgccgc cgaccgcctc
     4141 caagacggcg tctcgcgcgt caccgaggag atggtcgatc agctcgagca ggacatcgac
     4201 cgctacatgg accgcgtcga cctgccgccg aagttcgtga tcccgggcgg caacgagctg
     4261 tcggcccggc tcgacgtcgc gcgcgccgcg atccgccgtg ccgagcgccg cgtcagtgcc
     4321 ctacacgacg ccggcgagct tgccgacgac acggtgctgc gctacctcaa ccgccttgcc
     4381 gacgccgtct tcgcgctcgc ccgcttcgcc gaccgccccg atcccgagct gttccaaggc
     4441 agggacgcgg gatgaagcac gcggtcgcac ggcgtgtcgc cggagacggg ctgaagcacc
     4501 acatcgaggt cggcgggcac gagctgacct ccgacgagcc cgagcggctc ggcggcaccg
     4561 acgccggccc gagcccggag gagctgctcg ccgcgagcct cgcctcgtgc agcgcgatca
     4621 cgctcgagat gtacgcgcgg cgcaagggct gggacgtcgg cgacgtcgtc gtcgaggtcg
     4681 actacgagcc ggcgcagcgc gggtcgccga cgcgcttcgc gatgtcggtg cggctgccga
     4741 aggagctgcc cgaggagcag cgcgagcgcc tgatgcagat cgtcgccaag tgcccggtcc
     4801 accggacgct ggagggcgag gtcatgttcg acgagaagct cgagctcttc tagctcgcgc
     4861 ccttgctctt cgcgaggcgg ccgacctcct tgccgaacgc cgcctcgacc tcgcgcgtca
     4921 tcgtcagccc gaacatcgcc tggaccgcct gcgccgcgac cggccgcagg ccctcgatcg
     4981 accgcatcac ctccggccag cgctcctccg ggtagccggc ctcggtgaac ggcttccaga
     5041 cctcggtcag gtagagctgg acgaaggtgc gcgcgatcga ctcgcagcgc tcgttgacct
     5101 tcgccatcac ggcgatcgcc gcgtcgagcg acacgccgtt ggacacgacc tcctcagcga
     5161 ggtcgagcag cgacggcacc ggcgcctcgt agcggtcgcc gccgagcgcg accagcgccc
     5221 cgtgccgttc ggcctgagca agcatctcct cgccgccggc tccgccgaag cgctcgagca
     5281 gctcgtcgcg cgtgaagacc tgggacggct cggcggcgaa cggggcgcgt acgacctcgc
     5341 cgaacccgag cagctgctgg gccggacctc cggtcgtcgc gagcagccgc ttgatcccac
     5401 gcaggttgaa cccctcggcc tgcagctcct ggatcagctt gagccgtgcg acgtgctcgg
     5461 gcccgtagta gccgacccgg tcgcgcacct cgggcggcgg cagcagcccg cgcgcctgat
     5521 gcgagcggat cgtgcgtacc gtcatgccgg tctcgcgccc gagctcgtcg atggtcagct
     5581 cctgcggaga cgacccatta ctcaccatga cccgatgcta ccacgaccaa cgttacatca
     5641 agtgatgtca cgtggtacga tgggacaatg attcgcgatc ttctctacgc tggcgccgct
     5701 tcgcccccgg ccggaggcgc cgcgaccggc gagatcgcgc tcgccacagt cagcgccacg
     5761 ctgctcacgc tcggcctgct ctacctcggg gacggacacc gtcgcggcac cgtctcggta
     5821 ctgggcaagg tggccgcgtt cgcgacgcgc gtctccggcc agcccggctg ggccgcgttc
     5881 cccgccgcgc tggcgacgct ctcgctgctc acggcggtga tcggcatgta ctgggacatc
     5941 gcgctccacg tcaacgtcgg ccgcgacgag gggccgctcg ccaatcccgc ccactacttc
     6001 atcctcgccg ggctctacgg catcttcgcc gccgggttct tcgcgatggt cctgccgaag
     6061 gagaagccga gcgcgagctc ggtcaggctc ggcaaggact ggcacgcccc gctcggcggc
     6121 gtcctgatct gcgcctgcgg cgcgttcgcg ctgctggggt tcccgctcga cgacgtctgg
     6181 catcgcctgt tcggccagga cgtgacgctc tgggggccga cgcacctgat gctgatcggc
     6241 ggcgcgtcga tgacgctggt cgggatcgcg gtgctgcaga ccgagtctcg ccgctcgatg
     6301 gcggcggctg gcaagacgct cgacgaggcg ccgtggctcc acaagctgcg tctcgtcgcc
     6361 ctgaccggcg gcctgctgct cggcatgtcg accttccaag ccgagttcga cttcggcatc
     6421 gggcagttcc ggctgatctt ccagccgatg ctgatcatgc tcgccgccgg cgtcgttctg
     6481 gtcgccgcgc ggatctggct cggacgcggc gccgcactcg gcgcggtgct cttcttcatc
     6541 ctcgtgcgcg gcttcctcgc cgtgctggtc ggcccgatcc tcggcgagac cacgccccac
     6601 ttcccgctct acatcgcgtc cgcgctggta gtcgagctgg tcgcgctggc gatcgccgcc
     6661 gaccggcgtc cgctggcctt cggcctttgg gcaggcgtcc tgatcggaac cgtcggcctc
     6721 gccgcagagt ggggcttcag ccagctgtgg atgccgctgc cctggaccgc ggcgctgttc
     6781 cccgagggcg cgctgctcgg cttcgcgacc gccgtctgcg ccgcgctgat cggcgcctgg
     6841 gtcggtgccc atctcgcctc ggagacgatc ccgcgcacgc cgtcgctgcg cgccgccggg
     6901 gcgatcgccg cggccggcgt cggcgcgatg gtgctgtggg cgctctacac ccctcccggc
     6961 ccgccgacga cggtcgccgt gacgacgcgc gacatcgacc gcggcgccga gcggacgatt
     7021 gcggccacgg tccgcttcga cccaccgaac gccgccgagg acgccgagtg gctgacggcg
     7081 accgcctggc agggcgacgg gctcgtcgtc gaccgcctcg agcgcgtgcg cgagggcgtc
     7141 taccgcacga ccgagccgat ccccgtccac ggcaactgga agtcgatgat ccgcctccag
     7201 cgcggcaacg cgatcgatgc ggcgccgatc ttccttccgg acgacccggc gatccccgcg
     7261 cccgaggtgc ccgctcccgc ccagttcacg cgcgagttcg tgcccgacag cgaggtgctc
     7321 cagcgcgagc agaagaccga cgtcgcgggg acgctgacgg caatcgccta ctcggtggtc
     7381 gcggcgatcg cgctctcgct gctggcactg ctggcgtggg gactgcaccg cctcgccctc
     7441 acctccggca cgccgccgcg ctcgcgcgcg tcgcggaagg cagatcgacc ggcgatactg
     7501 gcggcgcggc gatgacctcc gcctccgtcc cagccccgtc ggctgttccc gatgtcgacc
     7561 atgtcgacgt cctgatcgtc ggcgccggcc tcagcggcat cggcgcggcc taccacctcc
     7621 agaccaagct cccgggcaag agctacgcga tcctcgaggg ccgcgacgcg atcggcggca
     7681 cctgggacct gttccgctat cccggcatcc ggtcggactc ggacctccac acgctcggct
     7741 acgcgttcaa gccctggacg gacgagaagg cgatcgccga cggcccggcg atcctcgctt
     7801 acatccgcca gaccgcgcgc gagaacggga tcgaccggcg gatccgcttc ggccaccgcg
     7861 tcgtgcgcgc cgagtggtcg agcgcggacg cccgctggac ggtcgaggca acccgcagcg
     7921 acgacggcgc gacggtcgcg atgacttgcg acttcctctt cgccgccggc ggctactacc
     7981 gctacgacga gggcttcacc ccccacttcg aggggcgcga gcgcttccag ggccagatcg
     8041 tccatccaca gcagtggccg gaggacctcg actacgcggg taagcgcgtc gtcgtgatcg
     8101 gaagcggcgc gacggcggtc acgctcgtcc ccgcgatggc cgaggaggcg gcgcacgtca
     8161 cgatgctgca gcgctcgccg acctacatcg tctcggtccc gtcgcgcgac ccgatcgcca
     8221 acgccctgcg ccgagtgctg ccgaatcgcg tcgcctactc ggtcgcgcgg cgcaagaacg
     8281 tcgcgctgca ggcgctcgtc tacaagctca gccggcgtcg ccccggcatc gtccgccggc
     8341 tgatccgcgc cgcggtcaag cgtcagctgc ccgacggcta cgcggtcgac acccacttca
     8401 agccgcacta cgacccctgg gaccagcggc tctgcgtcgt ccccgacgga gacctcttca
     8461 aggcgatctc gtcggcccgg gcgtcgatcg tcaccgaccg gatcgcgagc ttcaccgagc
     8521 gcggcgtcgc gctcgcctcg gggaccgagc tcgaggcgga catcgtcgtc acggcaaccg
     8581 gtctcaacct ggtcgcgttc ggtggtgtcg agtacgtcgt cgacggcgag ccggtcgtcc
     8641 tcgccgacac gttcgcctac cgcggagcga tgctcagcgg cgtgccgaac ttcgcgttcg
     8701 ccgtcggcta catcaacgcg tcctggaccc tgaaggtcga cctggtctgc gactggctct
     8761 gccggctgct cgctcacatg gactcgcacg gctacgcgca ggtcgtcccg gtcaacgacg
     8821 acacggacct gccgcgccgc ccgctgctcg acttctccgc cggctacgtc cagcgggcgc
     8881 tcgacagctt cccgaagcag ggccccgccg cgccgtggcg gatgagcatg agctaccgcg
     8941 aggacgtgcg caacctgcgc gacagcgagc tggtcgacgg caccctgcgc ttcacgcgcc
     9001 gcccggcggt cgagcgccag ccggacggcg tcgcggccta gccgcccgcc cgcttctccc
     9061 acatctgtat ctcgccgagg ctgcgatagc cgagccgctc gtagaccggg cgaccgaggt
     9121 cggtcgcctg cagcgtcgag gtcgtccgcc cgcgctcgcg cgcgtcggcc atcgcctggc
     9181 ccatcagcgc accggccagt ccgcggccgc gtgacagctc gcgcgtggcg accagccaga
     9241 cgctgcagtc gtcgccgtgg tcgaacgtga cgaggccgga cgcgagctcg ccgtcctcct
     9301 cgactccgta gaggtagagg ccgtcgcgcg cctcgaggcg atcgagcgcc ttcgcaaagg
     9361 cgtcgcgcat gccgtaggca tcgtcgttga ggcgggcgag ctcgatcgga tcgagctcgc
     9421 gcgtgaagcc tgtgggcgcg ggcggccgct cgatcgaatc caggtcgaga accatcgcct
     9481 cggggtcggc gtcgagcacg tggccgcggt cggccagcag cgccgcggtc tgctcgtcgg
     9541 agtcgggtac ccagacggtc caggcggcga tgcccgcggc ggcgtaggca gcctcgatct
     9601 cgtcgtaggc gtcgcgcagc gcgtcgcgat cccagtagac gacgtcgttg acgatcgagc
     9661 ggtcgggcac gcgcggtgtg atcgtcgtga cgagcccgtc gcgctcggcg acgcgcatcc
     9721 cgtccgacgc gccgtcgatc gtccgtgacc attgggcgag gctcgcgatc agccgccgcc
     9781 agccctctgc gtcgtcgatc tgcgccatgg cgcgatcata cgtcgcgtgc cgaccgccgc
     9841 cgagctgcgc gagctactgc tgacccccga gcaggcggcc gcgatggacg cgcccgggcg
     9901 gaccgacgcc gccgtgctgg tgcctatctt ccaccgcgac ggggacccgg tcgccgtctt
     9961 caccgagcgc agagcggacc tgcgccgtca tgcaggcgag atctcgtttc ccggcggccg
    10021 acaggacttc cccgacgagg acctgcggac gaccgccctg cgcgaggccg aggaggagat
    10081 cgggctcgag cgcgacgacg tcgagctggt cggcgcgctg ccgccggtcg gcaccttcgt
    10141 gaccggctac cgcgtctttc cgttcgtcgg gctgatcgag ccgggccgtg cctgggtgcc
    10201 gcaggagacc gaggtcgccc aggtcctcga gctgtcgctg gccgacctag tgcgcggcta
    10261 tgagagcaag cggctcacgc gccgcggcgt cccgatcaag acaccgacct acacggtcga
    10321 cggccatctc gtctggggcg ccacggcacg catcgtccag cacctgctca gacggctgga
    10381 gcctctgctc tagacgtcct tctcgaccgg cacgtcgacg aggttccccc actcggtcca
    10441 ggagccgtcg tagttcttga cgtcctgctg gccgagcagc tcgtgcagca cgaaccaggt
    10501 gtgtgccgag cgctcgccga tccggcagta ggcgatcgtc tccccgtcga gcacgccctt
    10561 gccgccgtag agctcacgca ggtcgtcggc cgacttgaag gtgccgtcct cgcgcaccgc
    10621 ctgcgcccac ggcaccgatg cggcgccggg gatgtggccc ccgcgctggg cgccctcctg
    10681 ctcgtatccc gccatcgcga tcagctcgcc ggagtactcc tgcggcgagc gcacgtcgac
    10741 caggttggtg cccttgccga gcgccgcgag gacctcgtcg cgcttggcac ggatcgcatc
    10801 gtcgccgggt ttggccgtaa aggacgcggc ggggtagctc ggcgcgtcgg acgtcaccgg
    10861 ccggccctcg gagatccact tctcgcgtgg cccgttcatc agcttgacgt tgtcgtggcc
    10921 gtagtacttc aggtaccagt acgtataggc ggcgaaccag ttgttgcggt cgccgtagag
    10981 gacgatcgtg tggtcgttgg agatgccgcg gctgccgaag agctcgccga aggcctgcgg
    11041 tccgagaaag tcgcgcttga cctggtcctg gaggtcggtc ttccagtcga agccgattgc
    11101 gcctgggatg tgggcctccg cgtagagcgc cgggttctcg tcgacctcga cgatccggat
    11161 gctgtcgtcg tcgaggtggt cctcgaccca ctgggtctcc accaggacgt ccttggcgta
    11221 ctcggccatt ggtcctcctt gctcgcctat gaaaatctgt aaacccggtc gggtttcgcc
    11281 tatatatgta gcaggctatg tccggcgttc gacgaccgcc ggttgagcgc cggctagctt
    11341 gggaaatgtg tctatcgaga tgaccgcgtt ggccacggcg gacctcgatc gggcggcgac
    11401 cgccgtacag gcactgctgg acgagcgcgg cgtgggcttc ggctccgaga cagacgcgtc
    11461 gcgcttcgtc gtcgacccgg tcccgcgtgt ggtcgagccg gagcagtggc aactgctccg
    11521 tcgcggcctg ccccagcgtg gccgggcgct cgccgcgttc accgccgacg tctacgggga
    11581 gcgtgccatc tgcgctgccg gcgtcgtgcc ggagcgagtg gcgaaatcgg ccgagtactt
    11641 cgagccggac ctggccgatc tgccaccggc gcgctgcggc tgggtcgccg gcttcgactt
    11701 ggtggctgag ggcgacggcg ccccgatggt gctcgaggac aaccatgcgc acgccatctg
    11761 ggatcgccta cgcgatcgcc gtgcgcgacg ccgtcgacgc ggccgcgccg gtcgacccgc
    11821 caccgaaccg gctcgacccg cgcgacgcgc tgccgttgct cgccgatgcg ctgcgcgcgt
    11881 cggcgccgga aggcgtcgag cgcccatcgg tcgcgctgct ctccgacggg ccgtcgaaca
    11941 gcgcctggta cgagcaccgt gccctcgcgg ccgcgctcga catcccgatc gtccgggaag
    12001 acgacctggc ggtccacgac ggccgtctcc acatgcgccg cgacggggcg cgtacgcagc
    12061 cggtcgacgt gatctaccgg cgcaccgacg aggaccgcct gcgcggagcc gacggagagc
    12121 cgacctggct cgccggcaag ctgctcgagc cgctgcgcgc cggcacgctc gcggtggtca
    12181 atccgttcgg agcggcggtc gcagacgaca agctcgtaca cgcctacgtc gacgacatga
    12241 tccgcttcta ctgcggcgag gagccgctgc tgaagtccgt tcccagctac gacctcggca
    12301 accccgcgat cttcgacgac gtcttcgagc gcctcgacga gctcgtcgtc aagccgcgca
    12361 gcggccacgg cggcgagggc gtcgtcgtct gtccgcgcgt cagcccgcag gcgcgcgacg
    12421 ccgtccgtcg tgcggtcgcc gcggaccccg acggctacat agcccaggag cccgttgacc
    12481 tctcgaccga gccgaccgtc tgcgacggcc ggctcgagcc gcgccacgtc gacctgcgac
    12541 ctttcgcgat cgccgtcggc gacgcggtgg tcaccgcgcc ggtcgcgctg acgcgcgtag
    12601 ccctggagcg cggcgccctg atcgtcaaca gctcgcgcaa cggcggcggc aaggacacat
    12661 ggctgctgag ctgaccgcga gccgtccgct gatcggcgtc acgacctccg aggtcaggtt
    12721 cgccgcgcgt atccagcaga ccgctcagtc ggagccgccg cggcgcgaga tggcgctcgg
    12781 actgacctac ctgctggcga tcgaggccgc gggcgggctg ccggtcgtga tgccaccgct
    12841 cgagctcgag gcgatcgagc cgctgctcga ccggctcagc ggcatctgcc tctccggcgg
    12901 accggacctc gacccgaccg cctactccga gcagcctcac ccccacctcg gcccgaccga
    12961 gcccgatctc gaccgcttcg agctcgagct ggcgcgccga gcggacacgc gcgggttgcc
    13021 gatcctggcg atctgccgcg gcatgcaggc gctcaacgtc gcgcgtggcg gatcgctgca
    13081 ccagcacctg ccggaccgcc ccggcgtggc gatcgaccac cgccagcgcg aggggggcga
    13141 ccacgcgatc cacgacgtga cgatcgcccc gggcagccgc ctggcgcgcc tgatgcgccg
    13201 ggatgcggcc gaggtcaact ccttccacca ccaggcgatc caccggatcg gcagcggtct
    13261 gcgggcggtc gcctggtcgc cggacggcgt gatcgagggc atcgaggcac cggcgcgtga
    13321 cttcgtcgtc ggcgtccagt ggcacgccga gagcctcgtc gagctgcgcg agcaggcgcg
    13381 gctgttccgc ggtctggtcg aggccgcgaa ccgcttcgag ggtcgcgacg ccggggcgcg
    13441 cgcagcatga acgaccgact ccccgactgg gcggtctggc aggggggtga cgagtacacg
    13501 ctcggagcag aggaggagct gatgctgctc gagccccacg actggtcgct cgcccagcag
    13561 atcgatcgcg tgctgccgca gctcccttcg tcgagtggag gaccacgtga cgcgcgagac
    13621 ccacacgtcg gcgctcgagc tgagcaccgg cgtccatgcg accgccgctg ccctgcggga
    13681 ggagctgatc tcgctgcgca cccggctcgg gcgcgagctg tcgctgctcg gcctgcgggc
    13741 ggcaagcgcc ggcacgcatc cctcgacggt ctggcacgag accgtcgtct cgaccggtcc
    13801 gcgctacgag gcggtctacg gctcgatgcg cgagctggcc cgtcgcgagc cgaccttcgg
    13861 cctgcacgtc cacgtggccg tgcgcggccc ggccgagggc atccgcctgt tcaaccggat
    13921 gcgcgtccat ctgccgctgc tgctggcgct gtcggtcaac tcgccgttct ggcagggccg
    13981 cgacacccgc ctcgcctcgg cccgcacacc gctgttccag gcctttccgc gcgtcggcat
    14041 cccgcgtgcg tttcgcgact acgaggactg ggtcggggcg gtcgacctgc tgatccgctg
    14101 cgacgcgttc cccgacccga ccttcctctg gtgggacgtg cgaccgcagc cgcgcttcgg
    14161 gaccgtcgag gtgcgcgtcc tcgacgcgca gacgacggcc agcgagtcgg ccgccctggc
    14221 ggcgctggtg cagtgcatcg cccggctcga ggtcgaggag ggatacgtcg ctaccgagct
    14281 gatcggggcg caggaggcgc tcgaggagaa ccgcttcatc gcggcgcgcg acggcatgga
    14341 cgctcgcctg atcgacccgc tcgacgagtg ccgcacgccg gtgaccgagc acctcgaccg
    14401 gctgctcgag gccagtgccc cgcacgccgc cgctcttggc tgcgagcggg agctcgagct
    14461 ggtcgaggcg atggcgcgcc agaccggcgc caagcggcag ctcgcgctgg cgcgcgacga
    14521 gggccgcctg ccagggttgg tcgaggcgct cgccgaccgc ttcctggccg acctggagcc
    14581 gcctgcggcg gagggcgggg cgcccgccgc gacggcggcg tagctagaac ggcggaccgt
    14641 cggagggccc gcgcgggcgc aggcgctcct cggcggcgag ctcctcgcgc aacccgtcct
    14701 cggtcagctc gggccggccc gaggcgcgtc gacgctcgtt gttggcctcg agcatctggt
    14761 cgacgtcgtc gagctcgagc tcgatctcca gctcgaccga ccgcgtcggc ttccagtcga
    14821 cctgctcggc gccggagccc ggataccact tgccgatcgc cagcaggatc aacaccccgg
    14881 cgatcaggct gcccgtcacc agccacgcga actcgtccat cgaccgagtc tagaccgccg
    14941 gtccagagcc gtgtcggccc tagactgccg ccgatgatcg acttcgagct gaccgacgag
    15001 cagcggctgg tgcgcgacac cgcacgcgag ttcgccgaca acgagatcgt gccgcgcgcc
    15061 cgcgacaacg accgctcggg ccgcttcgac accgagctgg tcgggaagct cgccgacatc
    15121 ggcttcatcg ggccgatcct gcctgaggag tacggcggcc gcgggatcga ctaccgcacc
    15181 tacgggctga tcgtcgagga gatcggccgc gcggactcct cggcgcgcac ggtcgtgtcg
    15241 gtcaacacct cgctggtcgg ctcggcgatc gccaagtggg gaaccgagga gcagaagcag
    15301 agcttcatcc cggggatctg ctccggcgag gcgctcggct gcttcggcct gaccgagccc
    15361 gatacgggct cggacgcggc gaacctcagc acgcgcgcgg agcgggtcga cggcggctgg
    15421 cggatcaccg gccagaagca gtggatctcg ctcggcaatc acgccaagtt cgcgctgatc
    15481 ttcgcccaga ccgacccgga gaaggcgcac cgcgggctcg gcgcgttcct cgttccgacc
    15541 gactcccccg gcttctcgtc ggctgagatc cacggcaagc tcggcctgca cgcatccgac
    15601 acggcggagc tcgcgctcga cggcgtcgag gtcggtgacg acgcgctgct cggtgaggtc
    15661 ggagacggct tcaaggtcgc gatgagcgca ctcgactcgg gccggttcag cgtgtcggcc
    15721 ggttgcgtcg gcatctgcca gggctgcgtc gacgcctcga cctcctacgc caaggagcgc
    15781 gtccagttcg gccgcccaat cgccgccttc cagctcgtgc aggcgatgat cgccgacatg
    15841 gtcgtcgaca cccatgcggc gcgcagcctg gtgtggaagg ccgcgtggct caaggacacc
    15901 gggcagccga acacgctcga gacctcggtc gccaagctgt tctccaccga ggcggcgatc
    15961 cgcagcgcga acaccgcgat ccaggtccac ggcgggtccg ggtacgtcga cgatcacccc
    16021 gtcgagcgct acctgcgcga cgcccgcgtc acgaccctct acgagggcac gtcgcagatc
    16081 cagaagctga tcatcggtcg cctgacgacc gggatcaacg cgatgacctg agtcgcggcg
    16141 gcgcgagcgc ggcccaggcg gcgtccgcga gcgccggctc ggcgtccgcc aggagcgacc
    16201 ggggggaacg ccccgactcg agcacgcccc gcgcgtactc ctgcgccggc ccccaccaga
    16261 tcgcgcacag caacgggatc tcgaggtcgc ggatcgcccc gtccgcggcc agcggcgcat
    16321 gccactccgc gagggccgcg aagaaccgct tgttcagcac ggcgacgtcg cctttgctga
    16381 cggcgacgac ctcggcctcg cgccgccgca tcaggaaccg cgctcgcgcc cggttggcct
    16441 cgacccagcc gaggtggtgg gcgacgccgg cgcgaacgcc ggcctcggcg tcctcgtcgc
    16501 gcagcgcggc gaggagcccg cgc
//
LOCUS       DATABV010000197        13067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2313669, whole genome shotgun sequence.
ACCESSION   DATABV010000197 DATABV010000000
VERSION     DATABV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13067
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313669"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="VHF88_09030"
     CDS             complement(<1..1538)
                     /locus_tag="VHF88_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2161947.1"
                     /translation="MPGDFDAVVIGAGHNGLVAANMLAERGWSVLVVEAQDLPGGAVK
                     SGEITEPGFTSDLFSAFYPLAAASPAIRALGLERQGLRWRRSELSVAHPQADGSCAGI
                     AATIDETAAMLERFAPGDGDAWRSLYAYWERAGAPFIDALLSPFPPLRGGARLAAALG
                     PRGLLRFGRFAVMPVRRLAEERFRGDGGAWLLAGNALHADLTPESAGGALFGWVLCGL
                     GQQHGFPVPEGGAGRLTAALVDRLRGHGGQVECGRQVERVLVRGRRAVGVRTADGQEI
                     AAGRAVLAGAGAPALFRDLVGEQHLPAGFVDDLRSFQYDNSTVKVDWSLDAPIPWIAE
                     DARRAGTIHVADGMDHLTETTAQLSRGLIADRPFLVVGQYSMVDETRQPPGRETAWAY
                     THVPQRVKGDARGELTGSWDEREVELFVARIEAEIERRAPGFGALIRARHVFTPRRLE
                     AANSNLVGGAVNSGTAQIHQQLVFRPVPGLGRAETPIRGLFLAGASAHPGGGVHGAAG
                     ANAAR"
     gene            1610..2068
                     /locus_tag="VHF88_09035"
     CDS             1610..2068
                     /locus_tag="VHF88_09035"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX2161948.1"
                     /translation="MATNERFMPVPPEAVWDALADAGGYGYWVVGSKEIRDADPEWPA
                     PGSKFHHTIGFGPFEIKDHTVSLEAERPKLLTLRAKGRPLGTARVTLTMTELDGGTLV
                     RMIENPDGLSSLLAINPVVQLLTFGRNAESLMRLEELAQRRNGSLDRPAA"
     gene            2065..3588
                     /locus_tag="VHF88_09040"
     CDS             2065..3588
                     /locus_tag="VHF88_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2161949.1"
                     /translation="MTSPTSPNESVWIDTGPPQPELARLDGPARADVVVIGGGIVGLT
                     TALLLQEAGSQVVLLEAQRIARGVSAYTTAKVSSQHGLKYAELASKHGPDAARTYGQA
                     NEAALAWIADRVDRHGIDCDFRRQPSFAYVADGSQRRQVEDETRAAIAAGLPASLTDA
                     TPLPYPVAAAVRFDDQAEFHPRKYLLALAEQLTAAGGQIYERSHAVEVDADRDEVAVK
                     TPGGRLTAGHAVVATHYPFLDRSFSFARVSAQRSYAIACRIAGSPPEGMHISGDSPTR
                     SIRAIPVGGEELLMVGGEGHKTGTGGDTEQRYAALEQFAREHWDVESVAYRWSTQDGS
                     TLDGMPYVGRLTPASNRVLMATGFAKWGMTGGTAAAQALADTIAGRENAAAKLWDPWR
                     KTLRQSAPKLVKENAQVAAHFVGDRLTKRGSRPIDSLAPGEGDIVEHDGEKVAAYRDD
                     DGALLAVSSRCNHLGCQLNWNRAERSWDCPCHGSRFAPDGRVLQGPAVHALERKPLR"
     gene            complement(3608..4027)
                     /locus_tag="VHF88_09045"
     CDS             complement(3608..4027)
                     /locus_tag="VHF88_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2161950.1"
                     /translation="MAKTAREVMTGGVECVGENDSILDAAKRLAELDVGAMPICGEDN
                     RLKGMLTDRDIVVKVLAQGKDPASTTAGELGEGKPVTIGADDGVDLAIRTMKDHKVRR
                     LPVIDGHDLIGVVSQADLARSVDEADVGDLVEAISAP"
     gene            4152..5336
                     /locus_tag="VHF88_09050"
     CDS             4152..5336
                     /locus_tag="VHF88_09050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX2161951.1"
                     /translation="MKAVTWQGKRNVSVETVPDPAIEQPTDAIVRVTSSGICGSDLHL
                     YEVLGAFIDAGDVLGHEPMGIVEEVGAEVTQIAPGDRVVIPFNIACGHCFMCDLGLQS
                     QCETTQVRDRDMGAALFGYTKLYGQVPGGQAEYLRVPQAQYGPIKVPEGPPDDRFVYL
                     SDVLPTSWQAVEYANVPDGGSVAVFGLGPIGEMCCRIAQQRGAGQVIGLDLVPERLER
                     ARGHGVVAIDVSEQQDLAAALRELTGGRGPDSVIDAVGMEAHGAPFGKLAQTLATLLP
                     DAAARKLMETAGVDRLSVLHAAIEVVRRGGTISLSGVYGGMTDPLPMLQLFDKQINLR
                     MGQANVKRWIDDILPLLSRDDDPLGVDAFATHRVPLDEAPNAYEIFQKKQDGAFKILL
                     QP"
     gene            5545..6258
                     /locus_tag="VHF88_09055"
     CDS             5545..6258
                     /locus_tag="VHF88_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX2161952.1"
                     /translation="MRVTSSLAGQPAREDMITSLLPLARRLASRYRHSGEAQEDLEQV
                     AYLGLVKAVDRYEPERGPLVRYAVPTILGELRRHFRDKGWGTHVPRSVQENLLDVTAA
                     TDKLLTQLGRTPTTQEIAGATGISSEDVIYALDAARSYTPAALDAPMAGDETESRALG
                     ETLGSTDEHYEFVELGEAIGPAFQILPEREQTIVRLRFFEDLTQSEIAERVGISQMHV
                     SRLLRRALDSLHNAVAPAA"
     gene            complement(6275..6544)
                     /locus_tag="VHF88_09060"
     CDS             complement(6275..6544)
                     /locus_tag="VHF88_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161953.1"
                     /translation="MADAPQRSDQPGAGDDAFLESLMDTGLYSMGAFFCDEHPDLVAD
                     VVAQSEAIEREGIRAYAAEHDLTQEACFEALLTGLAVRYYNAVAA"
     gene            6591..7352
                     /locus_tag="VHF88_09065"
     CDS             6591..7352
                     /locus_tag="VHF88_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX2161954.1"
                     /translation="MADRDAIVSYLDELLESPGFPDYGPNGLQVPGAERVELVVTGVS
                     AQLELFEQAAAAGAQLVLCHHGIVWGSRPAAISVQAKRRLQALFGADMSLLAYHLPLD
                     AHPEFGNNALVCAALGLEPAERIGSHEGRPIGFVGRSAEGVPLSDLRERCVRAFGGRE
                     PLIQGAGPQIVHSLGVITGAAAGYLGEAISLGLDGFLTGEPSEHAMADARENGLHFVA
                     AGHYASETFGVRRLGDLLAERFGVEHRFIDVPNPV"
     gene            7392..9182
                     /locus_tag="VHF88_09070"
     CDS             7392..9182
                     /locus_tag="VHF88_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX2161955.1"
                     /translation="MEAVTAASGTGSKTMADLLPLAAERYGDAPALKHKSGDQWVETS
                     YADLGRAVRETALGLIDLGLEHGDKVSILSHTRPEWTQANFAILSAGLVSVSIYQTNS
                     PEECHYVLEHSESRAVFAEDAEQLAKLRAVEDRLPKLEHIVVIDPSDESGDAIPLAEL
                     RERGAALDPGALEARTAAVTKDDLAINIYTSGTTGPPKGCLLTHGNYRDVTSMTEQMG
                     ALAAGDSVYLFLPLAHAFAKLIQFVALDLGGTIAYWEKDPQKIVPNLIEVKPTYFPSV
                     PRMFEKIYTLATNAAEDPEQLKQAVALGLKVRELQRNGQEVPAELQQAFDAADEKLYS
                     NVRALFGGQIRECVTGAAPIAQEILEFFYACGVPVMEGYGMTETSTVASSNTPERFRF
                     GSVGQPLPGVEAKVADDGELLLRGPNIFQGYYKNPDATASTLVDGWLHTGDLGRIDED
                     GFIYIVGRKKDIIITSGGKNITPANLENGLKQNRWVSQAVVVGDRRPYLVALITLDPE
                     EVPAFAAQHGLEPDQVASSEQMRAEVQKTVDAVNATVGPVEQIKYFEILPQDLSQETG
                     ELTPTLKVKRNVVADKFADVVDSIYARAGA"
     gene            complement(9205..10320)
                     /locus_tag="VHF88_09075"
     CDS             complement(9205..10320)
                     /locus_tag="VHF88_09075"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX2161956.1"
                     /translation="MGLLDYYKQFEDVDEEELNKARRERRAREKRLALERAPELDLSS
                     TEWPELPNSEVVNASIYTARGRVNGYPSRHAAGVRRMLAERHDVEPERIVVGNGAAEL
                     LQTAALTLMTAGDELVTPWPSYPLYPLMAARAGGRPVAVDTGPDGLDLDALRAAVSDR
                     TRIVMICNPNDPTGNYVPAERIAALAGDLPERAHLIVDEALVHFQEAEPLDAVLRLTD
                     AFPRLLVVRTFSKVYGLSGLRAGYAVGSNAAAKLLDSIAPVLGVNALTQAAVVQALKI
                     SGPEIERRRATVIEERHRLLAALSDMPIDVTSSQANFVWMAADGVSGAALANRLQRES
                     VTVAPGGPLGADDHVRAAIRDVAATNRLLSALEKALA"
     gene            complement(10326..10799)
                     /locus_tag="VHF88_09080"
     CDS             complement(10326..10799)
                     /locus_tag="VHF88_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161957.1"
                     /translation="MARNDPTESGGLFVRRPSEVRGVKYRNAPERKGGARQTADRGVA
                     FGLLALETLLCLSLFGPQPYFWFWFGSQVQHWTDSVSAGIATIMAGTLTSLLITVALA
                     KRVDNGWKLVRRAAGYKQERGALEVIFAISVAIAVVAFTFWFLIIAGPGPSIAPK"
     gene            complement(10831..12105)
                     /locus_tag="VHF88_09085"
     CDS             complement(10831..12105)
                     /locus_tag="VHF88_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2161958.1"
                     /translation="MGDVVKLEKPPAKGRKQPAAKARRRRPPRRSRTALVLGGGGFTG
                     GVYEIGALRALDLLAVNRTVNDFDVFVGTSAGSFVGSLVANGVTPEEMMRVVNQQPPS
                     PFRDVDRGQLMRPNYLEFAQSAALLPLRMLGVLRTVAGHFRELSVVDIAAELAQGLPS
                     GAYSTRGVEQYLDGVLAEDGRVNDFRRLNRELYLTATDIDTTERIVFGGDGWEDVSIA
                     KAVSASTALPMVYKPVEIAGRQLIDGGIRSTTNVDIAVERGAKFIVVVNPLVPYVNDF
                     QKQIPTAIGSRVRRVADMGFAQIGYQTFKLLAHQRLHEAVRQWKERYPGVDIVLIEPE
                     PNDQLMFETNIMNYTRRVEIARHGFESVTIKLAEDYDDLKAVCAKHGIEISATRVRKV
                     VGKLEREREKTAAWRRILEQTTGALLRQSEDG"
     gene            12177..>13067
                     /locus_tag="VHF88_09090"
     CDS             12177..>13067
                     /locus_tag="VHF88_09090"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX2161959.1"
                     /translation="MLDRSTTAIRPQIAALLALLTVLAASFAPSAAADERGLSNRLAG
                     AMGSAGSWSGAYVVNADSGRPVFSWRHTTPRILASNTKLFTTAAALARFGVEGTLGTE
                     VRGTGNLSGRGVYRGNLYLVGGGDPTFGSQSFSGSAYGGGATVEALAAQLDRAGIERV
                     AGRVVGDESAFDSLRGGPDSGYGTSIWVGPLSALGYNRGLGNENGSSFQANPPAFAAA
                     RLTDALRRRGIAVSGRPTARTTPASADILAAVQSPPMARLAALTNKPSDNYFAEMLVK
                     ALGMQIATPDRGASDESPSSD"
BASE COUNT         1944 a         4769 c         4542 g         1812 t
ORIGIN      
        1 cgggcggcgt tggcgcccgc cgcgccgtgg acgccgccgc cggggtgcgc ggatgccccc
       61 gcgaggaaga gcccgcggat cggcgtctcc gcgcgaccga ggcccggcac cggtcggaag
      121 accagctgct ggtggatctg cgccgtacct gagttcaccg ccccgccgac gaggttcgag
      181 ttggccgcct cgagccgccg cggggtgaac acgtggcgcg cgcggatcag cgccccaaag
      241 cccggcgccc gccgctcgat ctccgcctcg atccgcgcga cgaacagctc gacctcgcgc
      301 tcgtcccagc tgccggtgag ctcgccgcga gcgtcgccct tcacgcgctg cgggacgtgg
      361 gtgtatgccc aggcggtctc acggccgggg ggctggcgcg tctcgtcgac catcgagtac
      421 tggccgacga cgaggaacgg gcggtcggcg atcagcccgc gcgacagctg cgccgtcgtc
      481 tcggtcaggt ggtccatccc gtcggcgacg tggatcgttc cggcgcggcg cgcgtcctcg
      541 gcgatccaag ggatcggcgc gtcgagcgac cagtcgacct tgaccgtcga gttgtcgtac
      601 tggaaggagc gcaggtcgtc gacgaagccg gccggcagat gctgctcgcc gacgaggtcg
      661 cggaagagcg ccggcgcgcc cgcgccggcc agcaccgctc tgcctgctgc gatctcctgc
      721 ccgtcggcgg tgcggacgcc gaccgcccgc cgaccgcgca ccaggacgcg ctcgacctgc
      781 cgaccgcact ccacctggcc gccgtgccca cgcagccgat cgaccagcgc ggcggtcagg
      841 cgccccgcgc cgccctcggg gacggggaag ccgtgctgct ggccgagccc gcagaggacc
      901 cagccgaaca gggcgccgcc ggccgactcg ggagtgagat ccgcgtgcag ggcgttgccg
      961 gcgagcagcc acgcgccgcc gtcgccgcgg aagcgctcct ccgccaggcg ccgcaccggc
     1021 atcaccgcga agcggccgaa gcgcagcagc ccacggggcc cgagcgccgc cgccagtcgg
     1081 gcgccgccgc gcagcggcgg gaacgggctc agcagcgcgt ctatgaacgg cgcgccggcc
     1141 cgctcccagt acgcatagag cgatcgccag gcgtcgccgt cgccgggcgc gaagcgctcg
     1201 agcatcgccg ccgtctcgtc gatggtcgcc gcgatcccgg cacacgatcc gtcggcctgc
     1261 gggtgcgcca ccgagagctc cgaacggcgc cagcgcaggc cctgccgctc gagccccagc
     1321 gcgcggatcg ccggcgaggc ggcggcgagc ggatagaagg cgctgaacag gtcgctggtg
     1381 aagcccggct cggtgatctc gccgctcttg acagcgcctc cgggcaggtc ctgcgcctcg
     1441 accacgagga cgctccaccc gcgctcggcg agcatgttcg ccgcgaccag cccgttgtgg
     1501 ccggcgccga tcacgaccgc gtcgaagtct ccgggcacga ctcccctata cccgcgcgcc
     1561 cgaccgcgaa gccggtttct ccggcgacga gccggggtag cgatctgccg tggccacgaa
     1621 cgagcgcttc atgccggtgc ctccagaggc ggtctgggac gcactcgccg acgcgggcgg
     1681 ctacggctac tgggtcgtcg gctcgaagga gatccgcgac gccgaccccg agtggccggc
     1741 gcccggatcg aagttccacc acacgatcgg cttcggcccg ttcgagatca aggaccacac
     1801 cgtgtcgctc gaggccgagc gtcccaagct cctgacgctg cgtgcgaagg gccgcccgct
     1861 cggcacggcg cgggtcacgc taacgatgac cgagctcgac ggcggcacgc tcgtccggat
     1921 gatcgagaac cctgacgggc tctcgtcgct gctcgcgatc aaccctgtcg tccagctgct
     1981 gacgttcggc cgcaatgccg agtcgctgat gcggctcgag gagctggcac agcggcgcaa
     2041 cgggtcgctc gaccggccgg cggcatgacc tcgccgacga gcccgaacga gtctgtctgg
     2101 atcgacaccg gcccgccgca gccggagttg gcgcgcctcg acgggccggc gcgcgccgac
     2161 gtcgtcgtga tcggcggcgg catcgtcggg ctgacgacgg cgctgctgct ccaggaggcg
     2221 ggttcgcagg tggtgctgct cgaggctcag cggatcgcgc gcggcgtcag cgcgtacacg
     2281 accgccaagg tctcctcgca gcacggcttg aagtacgccg agctcgcctc gaagcacggc
     2341 ccggacgccg cgcgcaccta cggccaggcc aacgaggcgg cgctggcgtg gatcgccgac
     2401 cgcgtcgacc gccacggcat tgactgcgac ttccgccgcc agccgtcctt cgcgtacgtg
     2461 gccgacggct cgcagcggcg gcaggtcgag gacgagacgc gcgccgcgat cgccgccggc
     2521 ctgccggcca gcctgaccga cgcgacgccg cttccatacc cggtcgcggc ggcggtgcgc
     2581 ttcgacgacc aggccgagtt tcacccgcgc aagtacctgc tcgcgctcgc cgagcagttg
     2641 accgccgccg gcgggcagat ctacgagcgc tcgcacgccg tcgaggtcga cgccgaccgt
     2701 gacgaggtcg cggtcaagac acccgggggc cgcctgaccg ccggccacgc cgtagtcgcg
     2761 actcactacc cgttcctcga tcgctcgttc tcgttcgcgc gcgtcagcgc gcagcgttcc
     2821 tacgcgatcg cctgccggat cgccggctcg ccgccggagg gcatgcacat cagcggcgac
     2881 tccccgacgc gctcgatccg cgcgataccg gtcggcggcg aggagctgct gatggtgggc
     2941 ggcgagggcc acaagaccgg caccggcggc gacaccgagc agcgctacgc cgcgctcgag
     3001 cagttcgcgc gcgagcactg ggatgtcgag tcggtcgcct accgctggtc gacccaggac
     3061 ggctcgacgc tggacggcat gccctacgtc ggccgcctca cacccgcctc gaaccgcgtc
     3121 ctgatggcaa ccggcttcgc caagtggggc atgaccggcg ggaccgcggc ggcgcaggcg
     3181 ctcgccgaca cgatcgccgg ccgcgagaac gcagccgcga agctctggga cccctggcgc
     3241 aagaccctgc gccagtcagc gcccaagcta gtcaaggaga acgcccaggt cgccgcgcac
     3301 ttcgtcggcg accggctgac caagcgcggc agccggccga tcgacagcct cgcgcccggc
     3361 gagggcgaca tcgtcgagca cgacggcgag aaggtcgccg cctaccgcga tgacgacggc
     3421 gcgctgctcg ccgtgtcgtc gcgctgcaac cacctcggct gccagctcaa ctggaaccgc
     3481 gccgagcgct cctgggactg cccctgccac ggctcgcgct tcgcgccgga cggcagggtc
     3541 ctccagggcc ccgccgtcca cgcgctcgag cgcaagccgc tccgctagac gcgtctcgcg
     3601 gccgtcgcta cggcgccgag atcgcctcga ccaggtcgcc gacgtcggcc tcgtcgaccg
     3661 atcgggcgag gtccgcctgg ctgacgaccc caatcaggtc gtgcccgtcg atcaccggca
     3721 gccgccgcac cttgtggtcc ttcatcgtgc ggatcgccag gtcgacgccg tcgtcagcgc
     3781 cgatcgtgac cggcttgccc tcgcccagct cgccggcggt cgtagatgcc gggtccttgc
     3841 cctgggcgag caccttcacg acgatgtcgc ggtcggtcag catccccttg agccggttgt
     3901 cctcgccgca gatcggcatc gctccgacgt cgagctccgc gagccgcttc gcggcgtcga
     3961 ggatcgagtc gttctcgccg acgcactcca cgccgcccgt catgacctct cgcgctgtct
     4021 ttgccactgt ctcctcctcg attcgcttgt ccagcacccg tacccgctgc ccgagaggga
     4081 aaacgccgtt ttggcgtgcc ggccgggggt agacgaccgc tgagccgcaa gcgaccgcga
     4141 gaggaccgga gatgaaggca gtcacctggc agggcaagcg caacgtgagc gtcgagaccg
     4201 tccccgaccc ggcgatcgag cagccgaccg acgcgatcgt gcgcgtcacc tcgtcgggca
     4261 tctgcggctc cgacctgcac ctctacgagg tgctcggcgc cttcatcgac gccggcgacg
     4321 tgctcggcca cgagccgatg ggcatcgtcg aggaggtcgg cgcagaggtt acccagatcg
     4381 ctcccggcga ccgcgtcgtg atccccttca acatcgcctg cggccactgc ttcatgtgcg
     4441 acctcggact gcagtcgcag tgcgagacga cgcaggtccg cgaccgggac atgggcgccg
     4501 cgctgttcgg ctacaccaag ctctacggcc aggtccccgg cgggcaggcc gagtacctgc
     4561 gcgtcccgca ggcgcagtac gggccgatca aggtccccga gggcccgccg gacgatcgct
     4621 tcgtctacct ctccgacgtc ctcccgacct cctggcaggc cgtcgagtac gccaacgtgc
     4681 ccgacggcgg cagcgtggct gtcttcggcc tcggcccgat cggcgagatg tgctgccgga
     4741 tagcgcagca gcgcggcgcc gggcaggtga tcgggctcga ccttgtgccc gagcggctcg
     4801 agcgcgcccg cggccacggc gtcgtggcga tcgacgtcag cgagcagcag gatctcgccg
     4861 ccgcactgcg cgagctgacc ggcggccgcg gcccggacag cgtgatcgac gccgtcggca
     4921 tggaggcgca cggcgctccc ttcggcaagc tcgcccagac gctcgccacg ctgctgccgg
     4981 acgcggcggc gcgcaagctg atggagaccg ccggcgtgga ccgcctatcg gtgctgcacg
     5041 ccgcgatcga ggtcgtccgt cgcggcggga cgatctcgct gtcgggcgtc tacggaggaa
     5101 tgaccgaccc gctgccgatg ctgcagctct tcgacaagca gatcaacctg cgcatgggcc
     5161 aggccaacgt caagcgctgg atcgacgaca tcctgccact gttgagccgc gacgacgacc
     5221 cgctcggcgt ggacgccttc gcgacccatc gcgtgccgct cgacgaggcg cccaacgcct
     5281 acgagatctt ccagaagaag caggacggcg cgttcaagat cctgctccag ccgtgaccgg
     5341 acctgggcca ttgggccacg tttgaccgca ctcgcgcgtt gggtacaagc ccttcaggct
     5401 gttcaagaga gggtcgctgg gggcaacaga agtccggccg tctcacgaaa cgccacatct
     5461 gagggccgcg cagggaccgt gagcacccca cagggtgctc gacgggacct cccatgccag
     5521 tcaagagcag ggagaagcgc acaaatgcga gtaacctcca gcctcgctgg ccagccagcg
     5581 cgcgaggaca tgatcacgag tctgctgccg ctcgcgcggc ggctggccag ccgctaccgc
     5641 cactccggcg aggcgcagga ggacctcgag caggtcgcct acctcggcct cgtcaaggct
     5701 gtcgaccgct acgagccgga gcgcggaccg ttggtccgct acgcggtccc gacgatcctc
     5761 ggcgagctgc gccgccactt ccgcgacaag ggctggggca cacacgtccc gcgctcggtg
     5821 caggagaacc tgctcgacgt caccgcggcg accgacaagc tgctgaccca gctcggccgg
     5881 accccgacga cccaggagat cgcgggcgcg accgggatct cctcggagga cgtcatctac
     5941 gccctcgacg cggcacgctc ctacaccccc gccgcgctcg acgcgccgat ggccggcgat
     6001 gagactgagt cgcgcgctct cggcgagacg ctcggatcga ccgacgagca ctacgagttc
     6061 gtcgagctcg gcgaggcgat cgggccggcg ttccagatcc tgcccgagcg cgagcagacg
     6121 atcgtccgtc tgcgcttctt cgaggacctg acgcagtcgg agatcgccga gcgcgtcggg
     6181 atctcgcaga tgcacgtgtc ccgcctcctg cgccgggcgc tcgacagcct gcacaacgcc
     6241 gtcgcccccg ccgcgtgagc gggcggcgag ccggctacgc cgcgacggcg ttgtagtagc
     6301 gcaccgccaa gcccgtcagc agagcctcga agcaggcctc ctgggtgagg tcgtgctcgg
     6361 cggcgtaggc gcggatgcct tcgcgctcga tcgcctcgct ctgcgcgacg acgtcggcga
     6421 cgaggtcggg gtgctcgtcg cagaagaagg cgcccatcga gtagaggcct gtgtccatca
     6481 gcgactcgag gaaggcgtcg tcgccggcgc ctggctggtc gctgcgctgc ggtgcgtcgg
     6541 ccatcgcgct cagcgtaccc gcgccgacgc cccggtagcg tgtcggcgcg atggccgacc
     6601 gcgacgcgat cgtctcctac ctcgacgagc tgctcgagtc gccgggcttt cccgactacg
     6661 ggcccaacgg cctccaggtg cccggcgccg agcgcgtcga gctggtcgtc accggcgtct
     6721 cggcgcagct cgagctgttc gagcaggcgg ccgccgcagg cgcccaactg gtgctctgcc
     6781 atcacggcat cgtctggggc tcgcgccccg cggcgatcag cgtccaggcc aagcggcgcc
     6841 tccaggcgct gttcggcgcg gacatgtcgc tgctcgccta ccacctgccg ctcgacgcgc
     6901 accccgagtt cggcaacaac gcgctcgtct gcgccgcgct cgggctcgag ccggccgagc
     6961 ggatcggcag ccacgagggc cggccgatcg gcttcgtcgg ccgatcggcc gagggcgtcc
     7021 cgctctccga cttgcgcgag cgctgcgtcc gcgccttcgg cgggcgcgag ccgctgatcc
     7081 agggggccgg gccgcagatc gtgcacagcc tcggcgtgat caccggcgcg gccgccggat
     7141 acctcggcga ggcgatctcg ctcggcctcg acggctttct gaccggcgag ccgtctgagc
     7201 acgcgatggc cgacgcacgc gagaacgggc tccacttcgt cgccgccggc cactacgcca
     7261 gcgagacctt cggcgtccgg cggctcggcg atctgctcgc cgagcgcttc ggcgtcgagc
     7321 acagattcat cgacgtgccg aacccggtct agactccccc tcaacggagg cattcctggg
     7381 gaggagacac gatggaggca gtgacagccg cgtccggcac cggctcgaag acgatggcgg
     7441 acctgctgcc gctggcggcc gagcgctacg gcgacgcgcc cgcgctgaag cacaaatccg
     7501 gcgaccagtg ggtcgagacg agctacgcgg accttggccg cgccgtgcgc gagacggcgc
     7561 tcggcctgat cgacctcggc ctcgagcacg gcgacaaggt ctcgatcctc agccacaccc
     7621 gccccgagtg gacccaggcc aacttcgcga tcctctccgc cggcctggtc tcggtctcga
     7681 tctaccagac caactcgccg gaggagtgcc actacgtgct cgagcactcc gagtcgcggg
     7741 cagtcttcgc cgaggacgcc gagcagctcg ccaagctgcg cgccgtcgag gaccgcctgc
     7801 cgaagctcga gcacatcgtg gtcatcgacc cgtcggacga gagcggcgac gcgatcccgc
     7861 tcgccgagct gcgcgagcgc ggcgccgcgc tcgacccggg cgcgctcgag gcccgcacag
     7921 cagccgtcac caaggacgac ctcgcgatca acatctacac ctccggcacc accggcccgc
     7981 cgaagggatg cctgctgacc cacggcaact accgcgacgt cacgtcgatg accgagcaga
     8041 tgggcgcgct cgcggccggc gactcggtct acctcttcct cccgctcgcc cacgccttcg
     8101 ccaagctgat ccagttcgtc gcgctcgacc tcggcggcac gatcgcctac tgggagaagg
     8161 acccgcagaa gatcgtcccc aacctgatcg aggtcaagcc gacctacttc ccgtcggtgc
     8221 cgcggatgtt cgagaagatc tacacgctcg ccaccaacgc ggccgaggat cccgagcagc
     8281 tgaagcaggc cgtcgcgctc gggctgaagg tgcgtgagct gcagcgcaac ggtcaggagg
     8341 tcccggccga gctccagcag gcgttcgacg ccgccgacga gaagctctac tccaacgtgc
     8401 gcgcgctctt cggcggccag atccgcgagt gcgtgaccgg ggcggcgccg atcgcgcagg
     8461 agatcctcga gttcttctac gcctgcggcg tgccggtgat ggagggctac gggatgaccg
     8521 agacctcgac ggtcgcgagc tccaacacgc ccgagcggtt caggttcggc tcggtcggcc
     8581 agccgctgcc cggcgtcgag gcgaaggtcg ccgacgacgg cgagctgctg ctgcgcgggc
     8641 cgaacatctt ccagggctac tacaagaacc ccgacgccac cgcgagcacg ctcgtcgacg
     8701 gctggctgca caccggcgac ctcgggcgga tcgacgagga cggcttcatc tacatcgtcg
     8761 gccgcaagaa ggacatcatc atcacctcag gcggcaagaa catcacgccc gccaacctcg
     8821 agaacggcct caagcagaac cgctgggtgt cgcaggccgt cgtggtcggg gaccgtcggc
     8881 cgtatctcgt cgcactgatc acgctcgacc ccgaggaggt cccggcgttc gccgcgcagc
     8941 atgggctcga gccggaccag gtcgcgagct cggagcagat gcgggccgag gtccagaaga
     9001 ccgtcgacgc cgtcaacgcc accgtcggcc ccgtcgagca gatcaagtac ttcgagatcc
     9061 tgccgcagga tctgtcgcag gagaccggcg agctgacccc gacgctcaag gtcaagcgca
     9121 acgtcgtcgc cgacaagttc gccgacgtcg tcgactcgat ctacgctcgc gcgggcgctt
     9181 aagcctaccc cgctggtggg ctagctaggc gagggccttc tcgagcgcgg agaggaggcg
     9241 gttggtggcg gcgacgtcgc ggatcgcggc gcggacgtgg tcgtcggcgc cgagcgggcc
     9301 gccgggggcg acggtgacgg actcgcgctg gaggcggttg gcgagggcgg cgccggaaac
     9361 gccgtcggcg gccatccaga cgaagttggc ctgggaactg gtgacgtcga tcggcatgtc
     9421 ggagagcgcg gcgagcaggc ggtggcgctc ctcgatgacg gtggcgcggc ggcgctcgat
     9481 ctcggggccg ctgatcttca gggcttggac gacggcggcc tgagtgaggg cgttgacgcc
     9541 gaggacgggc gcgatcgagt cgagcagctt ggcggcggcg ttcgagccga cggcgtagcc
     9601 ggcgcggagg ccggagaggc cgtagacctt ggagaacgtg cggacgacga gcaggcgcgg
     9661 gaaggcgtcg gtcaggcgca ggacggcgtc gagcggctcg gcctcctgga agtggactag
     9721 cgcctcgtcg acgatcaggt gggcgcgctc gggaagatcg ccggcgagtg cggcgatccg
     9781 ctcggcaggg acgtagttgc cggtcgggtc gttggggttg cagatcatca cgatccgcgt
     9841 gcggtcgctg accgcggcgc gcagggcgtc gaggtcgagg ccgtcgggac ccgtgtcgac
     9901 ggcgaccggc cgcccgccgg cgcgcgccgc catcaacggg tagagcgggt agctcggcca
     9961 cggcgtgacc agctcgtcgc cggcggtcat cagcgtcagc gcggccgtct ggagcagctc
    10021 ggcggcgccg ttgccgacga cgatccgctc cggctcgacg tcgtggcgct cggcgagcat
    10081 ccggcgcacg cctgcggcgt gacggctcgg atagccgttg acgcgcccgc gcgctgtgta
    10141 gatcgacgcg ttgacgacct cggagttcgg cagctcgggc cactcggtgc tcgagaggtc
    10201 gagctccggg gcgcgctcga gcgccagccg cttctcacgc gcgcggcgct cgcggcgggc
    10261 cttgttgagc tcctcctcgt cgacgtcctc gaactgcttg tagtagtcga gcaagcccat
    10321 ccgacctact tgggcgcgat cgacgggccg gggcccgcga tgatgaggaa ccagaacgtg
    10381 aaggcgacga cggcgattgc cacgctgatc gcgaagatca cctcgagcgc gccacgctcc
    10441 tgcttgtagc cggcggcgcg ccggaccagc ttccagccgt tgtcgacccg cttggcgagc
    10501 gccacggtga tcagcagcga ggtcagcgtg ccggccatga tcgtcgcgat cccggccgag
    10561 acggagtctg tccaatgctg gacctgcgag ccgaaccaga accagaagta gggctgaggg
    10621 ccgaagaggc tgagacacag cagcgtctcc agcgcgagca ggccgaaggc caccccgcgg
    10681 tccgcggtct ggcgcgcgcc gcccttgcgc tcgggcgcat tgcggtactt gacgccgcgt
    10741 acctcgctcg ggcgacggac gaacaggccg ccgctctctg tcggatcgtt gcgcgccacg
    10801 tcgagacaga ctagggcacg gtagagctgc tcacccgtcc tcggactggc gcagcagggc
    10861 cccggtcgtc tgctcgagga tccgacgcca ggcggccgtc ttctcacgct cccgctcgag
    10921 cttgccgacg accttgcgca cgcgggtcgc cgagatctcg atgccgtgct tggcacagac
    10981 ggccttgagg tcgtcgtagt cctcggcgag cttgatcgtc accgactcga agccgtggcg
    11041 cgctatctcg acgcgccggg tgtagttcat gatgttcgtc tcgaacatca gctggtcatt
    11101 gggctccggc tcgatcaaca cgatgtcgac ccccggatag cgctccttcc actgccggac
    11161 cgcctcgtgt aggcgctggt gcgccagcag cttgaacgtc tgatagccga tctgggcgaa
    11221 gcccatgtcg gcgactcgcc gtacgcgact gccgatcgcc gtcgggatct gcttctggaa
    11281 gtcgttgacg taggggacca gcgggttgac gacgacgatg aacttggcac cgcgctcgac
    11341 cgcgatgtcg acgttggtcg tcgagcggat gccgccgtcg atcagctggc gcccggcgat
    11401 ctcgaccggc ttgtagacca tcggcagggc cgtcgaggcg ctgacggcct tggcgatcga
    11461 gacgtcttcc cagccgtcgc cgccgaagac gatccgttcg gtcgtgtcga tgtcggtcgc
    11521 ggtcaggtag agctcgcggt tcaagcggcg gaagtcgttg acgcggccgt cctcggcgag
    11581 cacgccgtcg aggtactgct cgacgccgcg cgtgctgtag gcgcccgagg gcaggccctg
    11641 ggcgagctcg gccgcgatgt cgaccaccga cagctcgcgg aagtgaccgg cgacggtgcg
    11701 cagcacgccg agcatgcgca gcggcagcag ggcggccgac tgggcgaact cgaggtagtt
    11761 gggccgcatc agctggccgc ggtcgacgtc ccggaacggg ctcggcggct gctggttgac
    11821 tacgcgcatc atctcctcgg gcgtaacccc gttcgccacg aggctgccga cgaacgatcc
    11881 tgcgctggtg ccaacgaaaa cgtcgaagtc gttgaccgtg cggttgaccg ccagcaggtc
    11941 gagcgcgcgc agggcgccga tctcgtagac cccgcccgtg aagccgccgc cgccgagcac
    12001 gagcgccgtc cgcgagcgtc gcggcggtcg gcgccggcgc gccttcgcgg ccggctgctt
    12061 cctgcccttc gccggaggct tctccagctt gacgacgtct cccatgccag gttgattatc
    12121 cccgagtggc ggtcggtcaa ccgctccggc cgcccatgta cggtgttctg ccgttgatgc
    12181 tcgacagatc gacaacggcg attcggccgc aaatcgccgc tctgctggcc ctcctgacgg
    12241 tcctcgccgc aagcttcgcc ccgtcggcgg ccgccgacga gcgcgggctc tcaaaccgcc
    12301 tcgccggcgc gatgggctct gccggaagct ggagcggggc ctacgtcgtc aacgccgaca
    12361 gcggccggcc ggtgttcagt tggcgccaca cgacgccgcg gatcctcgcc tcgaacacca
    12421 agctcttcac gaccgccgcc gcgctggcgc gcttcggcgt cgaggggacg ctcgggaccg
    12481 aggttcgcgg caccggcaac ctgagcggac gaggggtcta ccgcggcaac ctctacctcg
    12541 tcggcggcgg cgatcccacc ttcggcagcc agagcttcag cggcagcgcc tatggcggcg
    12601 gggcgaccgt cgaggcgctc gccgcgcagc tcgaccgcgc cggcatcgag cgcgtcgccg
    12661 gtcgcgtggt cggtgacgag tcggccttcg acagcctgcg cggcggtccc gactccggct
    12721 acggcacctc gatctgggtc gggccgctca gcgcgctcgg ctacaaccgc gggctcggca
    12781 acgaaaacgg ctcgagcttc caggcgaacc cgcccgcctt cgccgcggcg cggctgaccg
    12841 atgccctccg ccgccgcggc atcgccgtca gcggccgccc cacagcccgc accaccccgg
    12901 cctcagccga catcctcgcc gccgtgcagt cgccccccat ggcccgcctc gccgccctca
    12961 ccaacaagcc ctctgacaac tacttcgccg agatgctggt caaggccctc ggaatgcaaa
    13021 tcgccacccc cgatcgaggc gcgagcgacg agtccccgag cagcgat
//
LOCUS       DATABV010000198         5535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1510007, whole genome shotgun sequence.
ACCESSION   DATABV010000198 DATABV010000000
VERSION     DATABV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510007"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VHF88_09095"
     CDS             complement(<1..1059)
                     /locus_tag="VHF88_09095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX2161960.1"
                     /translation="MAAHRSRLPLAIAVAVVAATAATLLLRPRDGLIEPAAVNATAYF
                     SAADLQRAEEFRDLQRILALAGIALSGVVVALIALRPPGPVRRAVERAGRRPLLGGAA
                     VAAGIALVVAVVELPLALWRHERAVDFGLSTQSLGPWLGDVAKAAAVSALTAAIAGLL
                     VLALIRRFPRHWWAPSSVGVVALAAATLYLQPIVVAPLFNDFEPLPRGELRAEVLRLA
                     DRAGVDVGEVYRVDASRRTTAINAYVGGLGDTKRVVLYDNLIEDFPRAQVRSVVAHEL
                     GHVKHRDLWHGLLWVAIVAPAGMLCVQRLAERFARGPLDGAGKPGPAALPAVALAFAL
                     VSFALSLSGNYVSRAVEAR"
     gene            1048..1827
                     /locus_tag="VHF88_09100"
     CDS             1048..1827
                     /locus_tag="VHF88_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2161961.1"
                     /translation="MSSHPSDSNHPPGTQPARIAILSDVHGNLPAFETALADARAADV
                     DAIWCLGDLVGYGASPDECVALAQAECDVLLGGNHDLAVAGRIALTDFSPAAAAAARW
                     TREHASPDTLAVLETLDPYRENGRIGLYHGSPTDPIWEYVLSTDQADSCFDAMGPRVG
                     AIGHTHVALRFTRTDDGEVIGEQTPGGTEHDLGGDDWLVNPGSVGQPRDRDPRAAWLL
                     IDLDGWTASWRRVEYPIEVAAQAIAATDLPRHLADRLFIGQ"
     gene            1824..2417
                     /locus_tag="VHF88_09105"
     CDS             1824..2417
                     /locus_tag="VHF88_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161962.1"
                     /translation="MKLRLPHLLWTLAAVFALAVTGCGDDDEPQGRKLPPEVTQTLLQ
                     QLDSVGDRVAAGVPGACDDIYASEAEGGNIEPIDNALSSIPANVDPEIRSALEQSVER
                     LTQLVDQECAEIRSAAQDPDETVTEDDPPPVETVETETETVPVETTETTTTPPETTPT
                     TPPETPETPPNGNGPDGTGPPGQDRGGAEAPDSEDEE"
     gene            2417..3625
                     /locus_tag="VHF88_09110"
     CDS             2417..3625
                     /locus_tag="VHF88_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2161963.1"
                     /translation="MAQPGEIAGRYRIERRLGAGGMSTVFLAQDTVLERPVAVKLLAE
                     HLADDEAFVARFQREALAAARLQHPNVVQVFDYGLDAPSHRHFIVMEFVDGPSCADLL
                     RDRKRLDIEETVGIVRDACHGLDYAHRAGVVHRDVKPGNLLLSSDSGTTKLADFGIAK
                     AAEQTRITQVGSVLGTAAYLSPEQAHGDEAGPRSDTYSLGVCAYQLLTGRLPHEYGSL
                     TELALKQREEPIEPISELRPEVPLQLDLAIRVSLERDPADRYDSALDFARALEAGLHG
                     QETEATRRLAAAAVADPDATRALGDVTDATQALRTRRQPPVAPLPLDDGRREVAVPAP
                     DPKAAKRNARNRRWGQFLLLVAVLMAIAAVALVLLSSGDGGNGLAPVDRNSVQQQIEG
                     IEQFLRNNTR"
     gene            complement(3698..4873)
                     /gene="tgt"
                     /locus_tag="VHF88_09115"
     CDS             complement(3698..4873)
                     /gene="tgt"
                     /locus_tag="VHF88_09115"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX2161964.1"
                     /translation="MGQPFQIDARDGQARAGVLHCGHGDVRTPAFVPLASNASVRGLL
                     PGEVAELGYDMVLGNTFHLFITPGEDYVAKAGGLHRFMGWDGPIVTDSGGFQVFSMGH
                     GSVADEIKGRRDGRKSMIHEISEDGVRFQSYTDGGERFMGPETSMEVQAKLGSDIALA
                     FDECTPFHADRTYTLRAMERTHRWLDRCIAWHDEHAPASQLLYGIVQGGVYEELRRES
                     TDYVARAALDGVAIGGSLGQEKEQMHEVVGWALRGLPDERPRHLLGIGDVDDILHAVA
                     AGIDSFDCATPTRLARHGTALVADPTNRWRLDLTKSAAAGSEEPIAADCPCRACRSHT
                     RGYLHYLARHNELTGARLITLHNLTFMAELMSGVRAAIHAGELAAFTKRVLSGESPY"
     gene            complement(4896..5399)
                     /locus_tag="VHF88_09120"
     CDS             complement(4896..5399)
                     /locus_tag="VHF88_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2161965.1"
                     /translation="MSLLVRPEPFTQEFDRLFSRLFESDGSRGGSAVQRWMPPMDLME
                     ADDHYVLRADLPGLSEEDVAIEVQDGTLTISGERSAEREREEGGWFRVERSFGRFSRS
                     MTLPQGIAPDAVTAEFDRGVLTVRIPKPEERKPHRVAIHAGASNGSAANGEAPRTIEG
                     TASDSER"
BASE COUNT          837 a         2042 c         1956 g          700 t
ORIGIN      
        1 ccgcgcctcg actgcgcgcg atacgtagtt gccggagagc gaaagcgcga acgagacgag
       61 cgcgaacgcg agcgcgaccg ccggcagcgc tgctggacca ggcttgccgg cgccgtcgag
      121 cgggccacgc gcgaagcgct cggcgaggcg ctggacacag agcatgcccg ccggcgcgac
      181 gatcgcgacc cagagcaggc cgtgccagag gtcgcggtgc ttgacgtggc cgagctcgtg
      241 ggcgacgacc gagcgcacct gggcgcgcgg gaagtcctcg atcaggttgt cgtagagcac
      301 cacgcgcttg gtgtcgccga gcccgccgac gtaggcgttg atcgcggtcg tgcgccggct
      361 cgcatcgacg cggtagacct cgccgacgtc gacgccggcg cggtcggcga gccgcagcac
      421 ctcggcgcgc agctcgccgc gcggcagcgg ctcgaagtcg ttgaacagcg gggccacgac
      481 gatcggctgc aggtagagcg tcgcggcggc gagcgcgacc acgcccaccg agctcggcgc
      541 ccaccagtgg cgcgggaagc ggcggatcag cgcgagcacg agcaggccgg cgattgccgc
      601 ggtcagcgcc gacacggcgg ccgccttcgc gacgtcgccg agccacggac cgagcgactg
      661 ggtcgagagg ccgaagtcca ccgcccgctc gtggcgccag agggcgagcg gcagctcgac
      721 gaccgcgacg acgagggcga tccccgcagc gacggcggcg ccgccgagca gcggccggcg
      781 tccggcacgc tcgaccgcgc gcctcacagg ccccggcggg cgcagcgcga tcagcgcgac
      841 gaccaccccg ctgagcgcga tgccggctag cgcgaggatc cgctgaaggt cgcggaactc
      901 ctcggcgcgc tggagatcgg ctgcgctgaa gtaggctgtc gcattgacgg cagccggctc
      961 gatcaacccg tcgcgagggc gcagcagcag tgtcgcggca gtggccgcaa cgaccgccac
     1021 ggcgatcgcc agcggcagcc gagagcgatg agcagccacc cttctgacag taaccatcca
     1081 cccgggacac agccggcgcg gatcgcgatc ctctccgacg tgcacggcaa cctgccggcg
     1141 ttcgagaccg cgctggccga cgcgcgcgcg gcggacgtcg acgcgatctg gtgcctcggc
     1201 gacctcgtcg gctatggcgc gagccccgac gagtgcgtcg cgctcgccca ggcggagtgc
     1261 gacgtgctgc tcggaggcaa ccacgacctc gccgtcgctg gacggatcgc gctgaccgac
     1321 ttctcgcccg cggccgccgc ggcggcgcgc tggacgcgcg agcacgcgag ccccgacacg
     1381 ttggcggtgc tcgagacgct cgacccctac cgcgagaacg gccggatcgg gctctaccac
     1441 ggcagcccca ccgacccgat ctgggagtac gtgctctcga ccgaccaggc cgacagctgc
     1501 ttcgacgcaa tgggcccgcg cgtcggcgcg atcggccaca cgcacgtggc gctgcgcttc
     1561 acccgcaccg acgacggcga ggtgatcggc gagcagacgc ccggcggaac cgagcacgac
     1621 ctcggcggcg acgactggct cgtcaacccc ggcagcgtcg gccagccacg cgatcgcgac
     1681 ccgcgcgcgg cctggctgct aatcgacctc gacggctgga cggcgagctg gcggcgggtc
     1741 gagtacccga tcgaagtcgc cgcgcaggcg atcgccgcga ccgacctgcc caggcatctg
     1801 gccgaccggc tattcatcgg gcaatgaagc tgcgcctccc ccacctcctg tggacgctcg
     1861 cggcggtctt cgcgctggcc gtcacgggct gcggcgacga cgacgagccg cagggcagaa
     1921 agctgcctcc ggaggtgacc cagacgctgc tgcagcagct cgactcggtc ggcgaccgcg
     1981 tcgcggccgg ggtccccggg gcgtgcgacg acatctacgc gagcgaggcc gagggcggca
     2041 acatcgagcc gatcgacaac gcgctgagct cgatcccggc gaacgtcgac ccggagatcc
     2101 gctcggcgct cgagcagagc gtcgagcggt tgacccagct cgtcgaccag gagtgcgcgg
     2161 agatccgctc ggccgcccag gacccggatg agaccgtcac ggaggacgac ccaccgcccg
     2221 tcgagaccgt cgagacagag accgagacgg tgccggtcga gacgaccgag acgaccacca
     2281 caccgcccga gacgacgccg accacgccgc ccgagacgcc tgagacgccg cccaacggga
     2341 acggcccgga cggcaccggc ccgcccgggc aggaccgcgg cggcgccgag gcccccgact
     2401 cggaggacga ggagtgatgg cccagccggg cgagatcgcc ggccgctacc ggatcgagcg
     2461 ccgcctcggc gccggcggga tgtcgaccgt cttcctcgcc caggacaccg tgctcgagcg
     2521 cccggtcgcg gtcaagctgc tcgccgagca cctcgccgac gacgaggcct tcgtcgcccg
     2581 cttccagcgc gaggcgctcg ccgccgcgcg gctgcagcac ccgaacgtcg tccaggtctt
     2641 cgactacggc ctcgacgctc cctcgcaccg ccacttcatc gtgatggagt tcgtcgacgg
     2701 cccctcgtgc gccgacctgc tgcgcgaccg caagcggctc gacatcgagg agacggtcgg
     2761 gatcgtgcgc gacgcctgcc acggcctcga ctacgcccac cgcgccggcg tcgtccaccg
     2821 cgacgtcaag ccgggcaacc tcctgctctc gtcggactcg ggcaccacca agctggctga
     2881 cttcggcata gcgaaggcgg ccgagcagac gcggatcacc caggtcggct cggtgctcgg
     2941 caccgccgcc tacctgtcgc cggagcaggc ccacggcgac gaggccggcc cgcgctcgga
     3001 cacctactcg ctcggagtct gcgcatatca gctgctgaca ggacgccttc cgcacgagta
     3061 cggctcgctg accgagctgg cgctcaagca gcgcgaggag ccgatcgagc cgatctccga
     3121 gctgcggccg gaggtgccgc tgcagctcga cctggcgatc cgcgtgtcgc tcgagcgcga
     3181 ccctgcggac cgctacgaca gcgcgctcga cttcgcgcgt gcgctcgagg ccggtctgca
     3241 cggccaggag accgaggcga cgcgccggct cgcggcggct gcggtcgcgg accccgacgc
     3301 cacgcgcgcg ctcggcgacg tcaccgacgc cacgcaggcg ctgcgtacgc gccggcagcc
     3361 accggtggcg ccgctgccgc tcgacgacgg ccgccgggag gtcgcggtac cggcccccga
     3421 cccgaaggcc gccaagcgca acgcgcgcaa ccgccgctgg ggccagttcc tgctgctggt
     3481 cgcggtgctg atggcgatcg ccgcggtcgc gctcgtgctg ctctcgtccg gcgacggcgg
     3541 caacggcctc gcgccggtcg accgcaacag cgttcagcag cagatcgagg ggatcgagca
     3601 gttcctccgg aacaacacac gctagcccgg tcgaggccca cagcgaccgg ccaggcagcg
     3661 ccggggggtc cggggggcgt gagccccctg gatcgtctca gtagggcgat tcgccggaga
     3721 gcacacgctt cgtgaatgcg gccagctctc cggcatggat cgcggcccga accccgctca
     3781 tgagctcggc catgaaggtc aggttgtgca gcgtgatcag gcgcgcgccg gtcagctcgt
     3841 tgtggcgcgc cagatagtgc aggtagccgc gcgtgtgcga gcggcaggcc cggcacgggc
     3901 aatcggcggc gatcggctcc tcgctgccgg ccgcggcgct cttcgtcagg tcgagccgcc
     3961 agcggttggt cggatcggcc accagcgccg tgccgtggcg cgccagccgc gtcggcgtcg
     4021 cgcagtcgaa gctgtcgatc ccggcggcga cggcgtggag gatgtcgtcg acgtcgccga
     4081 tcccgagcag gtggcgcggc cgctcgtccg ggaggccgcg cagggcccag ccgacgacct
     4141 cgtgcatctg ctccttctcc tgaccgagcg agcctccgat cgcgacgccg tcgagcgccg
     4201 cccgcgcgac gtagtcggtc gattcgcggc gcagctcctc gtagacgccg ccctgaacga
     4261 tcccgtagag cagctggctg gctggcgcgt gctcgtcgtg ccaggcgatg cagcggtcga
     4321 gccaccggtg cgtgcgctcc atcgcgcgca gcgtgtaggt gcggtcggcg tggaacggcg
     4381 tgcactcgtc gaacgcgagc gcgatgtcgg agccgagctt cgcctggacc tccatcgacg
     4441 tctccgggcc catgaagcgc tcgccgccgt cggtgtagga ctggaagcgc acgccgtcct
     4501 cggagatctc gtggatcatg ctcttgcggc cgtcgcggcg acccttgatc tcgtcggcga
     4561 cgctgccgtg gcccatcgag aagacctgga agccgcctga gtcggtgacg atcgggccgt
     4621 cccagcccat gaagcggtgc aggcccccgg ccttcgcgac gtagtcctcg ccgggcgtta
     4681 tgaagagatg aaaggtgttg ccgaggacca tgtcgtagcc gagctcggcg acctcgccgg
     4741 gcagcaggcc gcgcaccgac gcgttcgacg ccagcggcac gaacgccggc gtgcgcacat
     4801 cgccgtgccc gcaatgcagg acgcccgcgc gggcctgtcc gtcgcgggcg tctatctgga
     4861 acggctgccc cacctgacgc agcctatgtg gagagctacc tctcgctgtc gctcgcggtg
     4921 ccctcgatcg tccgcggcgc ctcgccgttg gcggcggagc cgttggaggc cccggcgtgg
     4981 atcgcaaccc tgtgcggctt gcgctcctcc ggcttcggga tcctcaccgt cagcacgccg
     5041 cgatcgaact cagccgtcac cgcgtccggc gcgatgccct gcggcagggt catcgaccgg
     5101 ctgaagcggc cgaacgagcg ctcgacccgg aaccagccgc cctcctcgcg ctcgcgctcg
     5161 gccgagcgct cgcccgagat cgtcagcgtg ccgtcctgga cctcgatcgc cacatcctcc
     5221 tcgctcaggc cgggaaggtc ggcacggagg acgtagtggt cgtcggcctc catcaggtcc
     5281 atcggcggca tccagcgctg cacggcgctt ccaccgcgcg agccgtccga ctcgaacagc
     5341 cggctgaaca ggcggtcgaa ctcctgcgta aagggctctg gtctgactag aaggctcatc
     5401 gcaaatacct cctcgacaaa cagactacgc gttacttctt atgcgagcgc cagtataaga
     5461 cctgagtctc cgcgtgtcaa gtctgggggc ctacgagcag gctctttccg gtcatctcag
     5521 ctggctgggc gacgc
//
LOCUS       DATABV010000199        17677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1592003, whole genome shotgun sequence.
ACCESSION   DATABV010000199 DATABV010000000
VERSION     DATABV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17677
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592003"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VHF88_09125"
     CDS             complement(<1..283)
                     /locus_tag="VHF88_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161966.1"
                     /translation="MLRVRGGDSHEDEEDPERERPHGDPVGTRLDLRLRAWRAAARRY
                     WLSGDDDGTASVASLIIVPRGAGSGGLHRLSFVVPAGLADGLALKELRYV"
     gene            361..924
                     /locus_tag="VHF88_09130"
     CDS             361..924
                     /locus_tag="VHF88_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX2161967.1"
                     /translation="MKAKESRFQVHDELTAPEGSVPVLKGALAGGGQLSNIVGVLAGS
                     PATLRAYARFRSELRHGTLPLKTQQRIALAVAQHQGSEYALATLQRTARDAGLGLDEI
                     ALAREFDSRDEFEAVLLRYVKSLLTSDGPPPLHVHEEAREAGWEDEQILEAVAHVALA
                     SFGNLITRAGDVPNDGSAEEGRVARAA"
     gene            962..1336
                     /locus_tag="VHF88_09135"
     CDS             962..1336
                     /locus_tag="VHF88_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2161968.1"
                     /translation="MPTVIEPAEEPAVAPPPSGERSPCCALYHHAVELIGRRWTGAIL
                     LVLVEGGPLRFSEIRTLVPDVSDRLLSERLKELETEGIVVRHVYDETPVRVEYELTVK
                     GKALEPALTALKQWSHDYCPGR"
     gene            1376..2725
                     /locus_tag="VHF88_09140"
     CDS             1376..2725
                     /locus_tag="VHF88_09140"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX2161969.1"
                     /translation="MADQPKTAADVLGFVDEHDIRFVRLWFTDVLGQLKSFSINAAEL
                     DDAFEGGMGFDGSSITGFNAIEESDMIAMPDPATFAVLPWRPEDKGVARLFCDVLTPN
                     REPYEGDPRHVLRRALERAESMGFDTFNVGPELEYFYFRNARPADGVPEILDEGGYFD
                     LTTLDAGSDVRRETVLALEQLGIHVEYTHHEVGPSQHEIDMRYADALKMADDCMTYRI
                     TVKEYAMKYGWHATFMPKPLFGENGSGMHTHQSLFKGGQNAFFDADDQYYLSDVGKAF
                     IAGQLKHAREISSVFAQWVNSYKRLVPGYEAPVYCAWSRRNRSALVRVPLYHPGKEQA
                     TRMELRCPDPACNPYLTFAVLLHAGLEGIEKGYELPDPMEKNLYHLSPDERKRLGIEQ
                     LPETLGEAIELTSESELVLRAFGEHIFNRFVEIKRQEWEDYRVQVTQWELDRYMAVL"
     gene            complement(2722..5028)
                     /locus_tag="VHF88_09145"
     CDS             complement(2722..5028)
                     /locus_tag="VHF88_09145"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX2161970.1"
                     /translation="MSGGALALRLVVFGAFAAYGALHWSMLVASPSATVVTATIAATV
                     AGALGLALLDRSPTLPRRLLQPLAALIVLASLIAGLVAAGVAPRLLLPTGWGELADLL
                     RPALVGVQTVDWPYDGPDVWIRRTILLGAPLLLTTAAALAFWPVPAEMRRPLRGSALV
                     VLIALYAIAVITKDPGSPLLFGALLLALIAAWLWLPRRRGRELATAAAVVLAVALAAV
                     PVATALDGERPWFDYRSWSPFGDKRGIAFGWDHRYGPLDWPRQGTTLLRVRSESPHYW
                     KAATLNGFDGIRWHRPAGYEQPRDIGAELPFERDARPDPGGWSYGELNEDWVHQIRFT
                     VRSLSTELLVGAGLTYDVDGAAAMPSADGTTQLLSGTLERGDSYTIDSYEPNPTAAEM
                     AGAPTRYPQWVERYTELSLPASGSTLATGPSSVVVPPRGNRASPDERAEAEALLSGSA
                     YRQTYALARTLTAGSASVYDAVEDVESHLRESYDYDERVPERAYPLESFLFEDRRGYC
                     QQFSGAMALMLRMVGIPARVAVGFSPGTLDRDSGEYRVRDLDAHSWVEVYFAGIGWVT
                     FDPTPRAQDAEPAAGLTAVDGSLAAGQVRQGSSGPAPAPPADDGAESAQSPNAVAQTG
                     TEQSGAGGPPTALLLALALCALAAASAGAFAIRVRRRNGLDGERLAAAEIAELRSALE
                     RLGWNVSPATTLLALESQLARTAGPLAADYAQALRSYRFDPRSGRPPGPAERRKLRQA
                     LGAAAGRLGRLRALIAIPPGGPSNPSPS"
     gene            complement(5025..6137)
                     /locus_tag="VHF88_09150"
     CDS             complement(5025..6137)
                     /locus_tag="VHF88_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2161971.1"
                     /translation="MGRPIATALLGIGLCVVGAAFDSASLYLPGVAIVLLALVAATWV
                     WLAAIGARVVRAPGPPTVVEDEPFRVQVEAQLGWLPAPGGELADPLLKQETPLAGRRQ
                     AHIDALATLPQRGLVRLPPSELTVRDPLRLSSRRVASDEQAELLVLPRVEPVAAAADG
                     GASGAGGLPGIGSGPRPGASLRDDAADGLDLDGLRPYREGAPASRIHWATLARRGEMM
                     ERRFVSETAAAPLIVLDAGAPADEAALDAAVRAAASLCVALAGGGGVTLLLPGDRRPI
                     EIDHNLGAWNGVHKRLALVEPGATPSLGGLGPRSGALLWVTGAEAASLPRELEQLAAR
                     PRYLVAPTLPPGAIAAFAVADCSGTIVDHGHRSAVA"
     gene            complement(6144..7094)
                     /locus_tag="VHF88_09155"
     CDS             complement(6144..7094)
                     /locus_tag="VHF88_09155"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2161972.1"
                     /translation="MSTVPELDPRQVAELGARIERNVGTAVKAPAEVVREVMVALMAG
                     GHVLIEDFPGVGKTALARALARSIDADHARVQCTADLLPADLVGTDVYDQRENRFEFR
                     PGPVFANVVLVDEVNRASPKTQSGLLECMQERQVTVDRHTHELARPFLVLATQNPVEY
                     EGTYPLPEAQLDRFMVRVSLGYPSAEQEAQMLLDHARGDQVLEVTPVADLADVLAAQA
                     VTAAIHGSEALRRYIVALLAATRADPRVELGASPRAGLMLFRAAKAHAALDGRDHALP
                     DDVQQLAPAVLAHRLVLAPGSRPEQRATVIADALERVSAL"
     gene            7267..11748
                     /locus_tag="VHF88_09160"
     CDS             7267..11748
                     /locus_tag="VHF88_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX2161973.1"
                     /translation="MSRSWVSSSSSLSAGARLLICAAAVVASLLALGATQTAGQGAAP
                     DRDGRGASGAAGPTKPDGGGGVASVAGDELAHLRTRDSRTFVHESGSRVTHLYQGAVN
                     FRDGAGNWRPIDNELVPSAADPDSFQNAANRYRLSLPRDVGRDPVRMSAGGDWVDFSL
                     RDAAGAATVDGSVATYRDVREGMALAYTATADGVKEEIRLDSLSAARDLVFDMRVGAG
                     LTADMRSDGGVDVRGEDGRTRFVVAPPFMIDSAKNAEPSYAVSYELDRTATGYELAVR
                     PDRQWLESPKRRWPVVIDPTTTMPNPVNDCFITEGNPNTANCYNADLEVGYDAQGHGA
                     HDHRSLLRFPIGDSIPRGAVVLSAELDMRLFGRTTSNPVPVSVHTVKSSFDANDDRPW
                     TSSVTWNTWNGSNSWSAPGGDFDATPLATTTMGGQTGWQEWFLDPHRVQELVQRSRTD
                     NGFLLKSPGAAGPANYLQFASQDYDIADAPHLEIVWDERIGEKAEYTFERQRLSDRME
                     LAVNVGVGNLMLTATDLQIAGTGIDQAHSRTWNAITRRPWSRAGQWWNVDSAEDVRLE
                     TFDDGSAVFFGPTGVPIPFHKTGAAQWEPSPGYRATLATEGAGHKLTFPESGTILHFR
                     ADGLLTEQEDRNGNAIQFAYTNWNALDVVHDTQGRVTDYHYNAEGLIEEISDPGGRSV
                     HYEYDDDMLESYTDAEGNTTHYEYVNDEDLVEITDPRGNEIEFDYDGSHRVVSITRVN
                     DQPYVGDGPTWEFDYDTGDVRCPVRTVSNVMTDPRGKQTVYCRDRMFRVVKTIDANGN
                     ERSSSYSATNRVLGTTAPGSAQTTMSYDGQDNLTSVDEPAGEQSSFEYNSGTHPYFPT
                     KHTSPQGTGSVFAYDTAGNTTTIGDSSSPASQVEAKLEYNGQTGGSCPDDPTTKPGTL
                     RCAIDGKGNQTLYGYDDDGNLTSVTPELPLGDTTMTYDSLSRLATVEDGKGQTRSYSY
                     DALDRVTEIDYGGGNTVSFDYDANGNRIERIDSVHGTSTWEYDELNRRVEDDLPSGVT
                     DYTWDAASNLSSLTDGGGTVSYRYDDVNRLMDLAEPGGSCTTPVSLCTTFGYTNRDQR
                     ERTTYPNGVEQTVTFDSSDKPTRVRAAKGATVLTDFTYDYEMSPTTETKLRQSVTDKD
                     GNKTTYSYDFLDRLTSAVERNSSNAVVDDRSYDYDAASNRTSQVVNGATTSYAYNAAN
                     ELCWVHGSASNAACNAAPSGAITFGHDLNGNMTTTNAGVGFAYNVRDQTTSFDWPGAP
                     TTTFQYAGSGQSERTAVGGITQQNTLLGLSREGSTSWTRDPDGTLISQRASGGVRHYY
                     LSDGLGSIVGLTDSTGALTRSYKYDPYGLIRATTGSTPNPFKFTGQYHEAQGNLYKIG
                     ARYYAPTLGRWTQQDPLDQVGDLRDGNRYVYAGADPINRIDPTGLLSFDEIVREVQET
                     AGEVQRIYDDIQSLGRTRGGRALARCLLAGGVVTAVSKNPGWGLAVCGGVTVVTRGR"
     gene            12925..13173
                     /locus_tag="VHF88_09165"
     CDS             12925..13173
                     /locus_tag="VHF88_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161974.1"
                     /translation="MLLAESTTDGASVLLLIPAGLAIVARGVLMLVYTDRVLVPLARR
                     EAASPWAKFGMNTEYRYGAALLVVIGLGWMLGGAVYGL"
     gene            13163..13408
                     /locus_tag="VHF88_09170"
     CDS             13163..13408
                     /locus_tag="VHF88_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161975.1"
                     /translation="MGSERPPAKRRRTQTPARPFWLAASIFWIGGGVAVIAGKVLLGA
                     ALLLVAAAALLILRSLHRRAKGSVDRDHASILDMLGP"
     gene            13513..14043
                     /locus_tag="VHF88_09175"
     CDS             13513..14043
                     /locus_tag="VHF88_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161976.1"
                     /translation="MDEGRDNPKDLEIFEVRRQLMSHAALSAVLAVASTVAVLVIFDW
                     DASEVAVWGGIGAVVWTVVAFFTWTAAMLPRGDLLPPPPDVVLGRRGGLIQDAIRLVA
                     VVMACVGIAWLATEMDAGAFSIPGQFIGYACASLAGALWVAWWERRHGQTVVARYERE
                     DDDELYATPLAPDADG"
     gene            14331..14636
                     /locus_tag="VHF88_09180"
     CDS             14331..14636
                     /locus_tag="VHF88_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161977.1"
                     /translation="MTTDDLKQLVRDVELPRQKVYRAGDVQYVPAWRLVAVASRSYGL
                     GEDAEKQVDALVVELGGKRIDVVDPWRVIPDLIKRLLGRPIPPREDLYELPTTMLET"
     gene            complement(14841..15380)
                     /locus_tag="VHF88_09185"
     CDS             complement(14841..15380)
                     /locus_tag="VHF88_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161978.1"
                     /translation="MRAILVAALAALAASWLLTHSETVQFAVLLAFALAVRRFADGRD
                     APDDRSAEATPPWRVFGLSLVLLLPWGVAVFFLSGWIDLRFFWPFAFLWPLLEVQAAL
                     VRRDADRYGTDHWKPEQVFPDAVIAALITIPLITAIAFFLGDASLPEALAGGAACGLI
                     VFAMATVFIRLDRRSRSTG"
     gene            complement(15413..>17677)
                     /locus_tag="VHF88_09190"
     CDS             complement(15413..>17677)
                     /locus_tag="VHF88_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2161979.1"
                     /translation="GQQGGRGPSESGPAGAGGQSPAETTGSAQGGAASPQSGQPAGCG
                     GPTTPVAGQPEGYAPFVGPICSRDGKAAIVTAYLKGDGEADTILDPIQEWRDQVSDPG
                     GGLEVKITGGAGYAADAIEVFESIDGTLLLAALSLVIFLLIVIYRSPIFFLIPLLAVV
                     FAEMLSRSVGYGISELGVTINGQSSSIMSILVLGAGTDYALLIVARYREELHHTADKH
                     QAMQTALASAGPAIFASAATVIAALFCLTIAKVNGTSGLGPIGAMGVACAALSMLTLL
                     PALLTIFGRRAFWPFVPHTPETAPTEEGVPGWSLRNITEGPRVTALGSVILASLLVIV
                     LLPLVLLNLVVKLIVALVVRWFTDWRFPSLIVGPLDKRVFRPYELRRYRREHLADATH
                     GFWKRVGDWVARGRNRVLAGSLAVLFVLCCGLAFFSTDLTSNDSYRTEVESVQGQEIL
                     AESFAAGSAAPTDIIVADPGRVEAVTRAAEQVPGVESVTPPVAQGEGGVLVQATLEPT
                     PYSTEAYDIVEPLRDAVRDVAPDTLVGGPTAVEFDLREASAWDSIVIPPIVLVVVFLI
                     LVLLLRAVVAPLILIATVILSFAAALGVGYFAFDMVFGFPGSDPGLPLFAFVFLVALG
                     VDYNIFLIARAREETLKHGTRDGVLRALAVTGGVITSAGIVLAGTFSVLAVLPLVFLT
                     EIGFVVAFGVLLDTFLVRSVLVPALALKIGPKFWWPSELAKTDGGANGGSRTASSDGR
                     AIGEPQPLPADAKT"
BASE COUNT         2948 a         5959 c         6218 g         2552 t
ORIGIN      
        1 agacgtagcg cagctctttc agcgcgagcc cgtcagctaa ccccgccggc acgacgaaag
       61 agagccgatg cagaccaccg ctgcccgccc cgcgcggcac gatgatcaga gaagcgacgg
      121 acgccgtacc gtcgtcatca ccggacagcc agtaccgccg cgccgccgcc cgccacgcgc
      181 gcaggcgcag atccaggcgc gtcccgaccg gatcgccatg tgggcgttcg cgctcgggat
      241 cttcctcgtc ttcatggcta tcgccaccgc gaacgcgtag cacctcatta ggcgccggtt
      301 cgcggcgccg cctcggctat ccttacttcc ggtaagcaac cggcagaaag agaagcgccg
      361 atgaaggcca aggagagcag gttccaggtc cacgacgagc tgaccgcacc cgagggcagc
      421 gtgccggtgc tcaaaggcgc cctcgccggc ggcgggcagc tctccaacat cgtcggcgtc
      481 ctcgcgggct cgcccgccac gctgcgcgcc tacgcgcgct tccgctccga gctgcgtcac
      541 ggcacgctgc cgttgaagac ccagcagcgg atcgccctcg ccgtcgccca gcatcaggga
      601 agcgagtacg cgctcgccac gctccagcgc acggcgcgcg acgccgggct cggtctcgac
      661 gagatcgcgc tggcgcgcga gttcgactcg cgcgacgagt tcgaggcggt gctgctgcgc
      721 tacgtcaagt cgctactcac cagcgacggc ccacccccgc tgcacgtgca cgaggaggcg
      781 cgcgaggcgg gctgggagga cgagcagatc ctcgaggcgg tcgcccacgt ggcgcttgcg
      841 agcttcggga acctgatcac gcgcgccggt gacgtgccca acgacggctc ggccgaggaa
      901 ggacgagtgg ctcgggcggc ctgagcccgg tcctctagga ttcgagacca tgggcgcagg
      961 catgcctacg gtcatcgagc cggccgagga gccggcggta gccccacccc caagcggcga
     1021 gcggtcgccg tgttgcgctc tctatcacca cgccgtcgag ctgatcggca ggcgctggac
     1081 cggcgccatc ctgctggtcc tggttgaggg cgggccgttg cgcttctccg agatccggac
     1141 gctcgtcccc gacgtctcgg accggctgct ttccgagcgg ctgaaggagc tggagaccga
     1201 gggcatcgtc gtccgccacg tctacgacga gacgccggtc cgcgtcgagt atgagctgac
     1261 cgtcaagggc aaggcgctgg agccggcgct gacggcgctc aagcagtggt cgcacgacta
     1321 ctgccccggc cgttagcctc tgcggcttca agcgccgaga cgactagggg accgcatggc
     1381 cgaccagccg aagaccgccg ctgacgtact gggcttcgtc gacgagcacg acatccgctt
     1441 cgtccggctc tggttcacag acgtgctcgg ccagctcaag agcttctcga tcaacgccgc
     1501 ggagctcgac gacgccttcg agggcggcat gggcttcgac ggctcatcga tcaccggctt
     1561 caacgcgatc gaggagtcgg acatgatcgc gatgcccgac ccggcaacat tcgccgtgct
     1621 gccctggcgg ccggaggaca aaggcgtcgc acgcctcttc tgcgacgtgt tgacgccgaa
     1681 tcgcgagccc tacgagggcg atccgcgcca cgtcctgcgc cgcgcactcg agcgcgccga
     1741 gagcatgggc ttcgacacct tcaacgtcgg tccggagctc gagtacttct acttccgcaa
     1801 cgcgcggccg gccgacgggg tgccggagat cctcgacgag ggcggctact tcgacctaac
     1861 cacgctcgac gccggctccg acgtgcgccg tgagaccgtg ctcgcgctcg agcagctcgg
     1921 gatccacgtc gagtacaccc accacgaggt cggcccgtcc cagcacgaga tcgacatgcg
     1981 ctacgccgac gcgctcaaga tggccgacga ctgcatgacc taccgcatca ctgtcaagga
     2041 gtacgcgatg aagtacggct ggcacgccac cttcatgccg aagccgctgt tcggcgagaa
     2101 cggctccggg atgcacacgc accagtcact cttcaagggc ggtcagaacg cgttcttcga
     2161 cgccgacgac cagtactacc tgtcggacgt cggcaaggcg ttcatcgccg ggcagctcaa
     2221 gcacgcgcgc gagatctctt cggtcttcgc gcagtgggtc aactcctaca agcggctggt
     2281 gcctggctac gaggcgccgg tctactgcgc ctggtcgcgc cgcaaccgct cggccctggt
     2341 gcgggtgccg ctctaccacc ccggcaagga gcaggcgacg cggatggagc tgcgctgccc
     2401 cgacccggcc tgcaacccgt atttgacctt cgcggtcctg ctgcatgccg ggctcgaggg
     2461 gatcgagaag ggctacgagc tgcccgaccc gatggagaag aacctctacc acctgtcgcc
     2521 cgacgagcgc aagcggctcg gcatcgagca gctgcccgag acgctcggcg aggcgatcga
     2581 gctgacctcg gagtcggagc tcgtcctgcg cgccttcggc gagcacatct tcaaccgctt
     2641 cgtcgagatc aagcgccagg agtgggagga ctaccgcgtc caggtgaccc agtgggagct
     2701 ggaccgttac atggccgtcc tctaggacgg cgaggggtta gacggcccgc ccggcgggat
     2761 cgcgatcaac gcgcgcaggc ggccgagccg gccggcggcc gcgccgagtg cctggcggag
     2821 cttccggcgc tcggccggcc cgggtggcct cccgctgcgc ggatcgaagc gatagctgcg
     2881 cagcgcttgg gcgtagtcgg cggccagcgg gcctgccgtg cgggcgagct ggctctcgag
     2941 ggcgagcagc gtggtggcag gggagacgtt ccagccgaga cgctcaagcg cactgcgcag
     3001 ctccgcgatc tcggccgccg cgaggcgctc gccgtccagg ccattgcgac ggcggacacg
     3061 gatcgcgaac gcaccggctg acgccgccgc gagagcgcag agcgcgagcg cgagcagcag
     3121 cgccgtcggc gggccgcccg cgccgctctg ctcggtcccc gtctgcgcga cggcgttggg
     3181 gctctgggcc gactccgcgc cgtcgtcggc gggtggcgcc ggcgcggggc ccgacgagcc
     3241 ttggcgtacc tggccggcgg cgagactgcc atcgacagcc gtcaggcccg ctgccggctc
     3301 tgcgtcctga gctcgcggcg tcgggtcgaa agtcacccag ccgatcccgg cgaagtagac
     3361 ctcgacccat gagtgggcgt cgaggtcgcg cacgcggtac tcgccgctgt cgcggtccag
     3421 cgtgcccggc gaaaacccga cggcgacacg tgccgggatc ccgaccatgc gcagcatcag
     3481 cgccatcgcg ccggagaact gctggcagta gccgcgccgg tcctcgaaca ggaagctctc
     3541 cagtggatac gcgcgctccg ggactcgctc gtcgtagtcg tagctctcgc gcaggtggct
     3601 ctcgacgtcc tcgaccgcgt cgtagaccga cgcgctgccg gcggtcagcg tccgggccag
     3661 cgcataggtc tgccgatagg cggagcccga gagcagcgcc tcggcctcgg cgcgctcgtc
     3721 cggcgaggcg cggttgccgc gtggcgggac aaccacggag ctgggaccgg tcgcgagggt
     3781 cgaaccgctc gccggcaagg agagctccgt gtagcgctcg acccactgcg gatagcgcgt
     3841 cggagccccg gccatctcgg cggcggtcgg gttcggctcg taggagtcga tcgtgtagct
     3901 gtcgccgcgc tcgagcgtcc cgctcagcag ctgggtcgtg ccgtccgccg acggcatggc
     3961 ggcagcgccg tcgacgtcgt aggtcaggcc ggcgccgacc agcagctcgg tggacagcga
     4021 cctgaccgtg aagcggatct ggtggaccca gtcctcgttg agctccccgt agctccaccc
     4081 acctgggtcg ggacgcgcgt cgcgctcgaa cggcagctcc gcgccgatgt cgcgcggctg
     4141 ctcgtagccg gccggcctgt gccagcggat gccgtcgaag ccgttgagcg tcgcggcctt
     4201 ccagtagtgc gggctctccg aacggacccg gagcaacgtc gtgccttgac gcggccagtc
     4261 gagcggccca tagcggtggt cccagccgaa ggcgatcccg cgcttgtcgc cgaaggggga
     4321 ccacgagcgg tagtcgaacc agggtcgctc gccgtcgagg gccgtggcga ccggcactgc
     4381 cgccagcgcg acggccagca ccacggctgc cgcggtcgcg agctcgcggc cgcgccggcg
     4441 cggcagccag agccaggcgg cgatcagcgc cagcagcagc gccccgaaca acagtggact
     4501 gccggggtcc ttggtgatga cggcgatcgc atagagcgcg atcaagacca ccagagccga
     4561 cccgcgcagc ggccgacgca tctccgcggg cacgggccag aaggcgagtg cggccgccgt
     4621 ggtgagcagc agcggcgcgc cgagcaggat cgtccgccgg atccagacgt ccggtccgtc
     4681 gtagggccag tcgaccgtct ggacaccgac cagggcgggg cggagcagat cggccagctc
     4741 cccccaaccg gtcgggagca gcaggcgggg cgccactccg gcggcgacca ggccggcgat
     4801 cagcgacgcg aggacgatca gtgcggcgag cggctggagg aggcgccgcg gcagcgtcgg
     4861 cgagcggtcg agcagcgcga ggccgagggc gccggcgacg gtcgcggcaa tcgtcgccgt
     4921 cacgaccgtg gccgatggcg acgccacgag catcgaccag tggagcgcgc cgtaggcggc
     4981 aaaggcgccg aatacgacca gccgcagggc gagcgcgccg ccgctcacgc gaccgcgctc
     5041 cgatgtccgt ggtcgacgat cgtcccgctg caatcggcga cggcgaacgc cgcgatcgcg
     5101 ccgggcggca gtgtcggcgc gaccaggtag cgcggacgcg cggcgagctg ttcgagctcg
     5161 cgcggcagcg acgcggcctc ggcgccggtg acccacagca gcgcaccgga gcgcggcccg
     5221 agcccaccca ggctcggggt ggcccccggc tcgacgagcg ccagccgctt gtgcacgccg
     5281 ttccacgcgc cgaggttgtg gtcgatctcg atcggacgtc gatcgccggg cagcagcagc
     5341 gtgacgccgc caccgccggc aagggcgaca cagagcgacg ccgccgcgcg caccgcggcg
     5401 tcgagcgctg cctcgtcggc gggcgcgccc gcgtcgagga cgatcagcgg cgccgcggcg
     5461 gtctcggaca cgaagcgccg ctccatcatc tcgccgcggc gagccagcgt cgcccagtgg
     5521 attcgggaag ccggcgctcc ctcgcgatag gggcgcaggc cgtcgaggtc gaggccgtcg
     5581 gcggcgtcgt cgcgcaggct cgcgccgggt cggggcccgc tcccgatacc gggcaacccg
     5641 cccgcgccgg acgcgccacc gtccgcggct gccgccaccg gctcgacgcg cggcagcacg
     5701 agcagctccg cctgctcgtc agaggcgact cgccgcgacg agaggcggag cgggtcgcgg
     5761 acggtcagct cgctcggcgg gagccggacc aggccgcgct gcggcagcgt cgcgagcgcg
     5821 tcgatgtgag cttggcggcg tccggcgagc ggcgtctcct gcttcaggag cggatcggcg
     5881 agctcaccgc ccggggcggg cagccagccg agctgcgcct cgacctgcac ccggaacggt
     5941 tcgtcctcga ccaccgttgg tgggccgggt gcgcgtacga cgcgcgcccc gatcgcggcg
     6001 agccacaccc aggtcgccgc gaccagcgcc agcaggacga tcgcgactcc cggcaggtag
     6061 agcgaggcgg agtcgaaggc ggcgccgacg acgcagagcc cgatgcccag cagcgccgtt
     6121 gcgatcggcc gccccacgat gcctcagagg gcggagaccc gctcgagcgc gtcggcgatc
     6181 acggttgcgc gctgctcggg ccgcgatccg ggcgcgagga cgaggcggtg ggcgagcacc
     6241 gcgggggcga gctgctgcac gtcgtctggc agcgcgtgat cgcgcccgtc gagtgcggcg
     6301 tgcgccttcg cggcgcggaa cagcatcagg ccggcgcgcg ggctggcgcc cagctcgacg
     6361 cgcggatccg ctcgggtggc ggcgagcaga gcgacgatgt agcggcgcag cgcctcgctg
     6421 ccgtggatcg cggcggtcac ggcctgcgcg gcgagcacgt cggcgaggtc ggcgacgggc
     6481 gtcacctcaa gcacctgatc cccgcgggcg tggtcgagca gcatctgcgc ctcctgctcc
     6541 gccgacgggt agccgagcga gacgcgcacc atgaagcggt caagctgtgc ttcgggaagc
     6601 gggtaggtgc cctcgtactc gaccgggttc tgcgtcgcca gcaccagaaa cgggcgcgcc
     6661 agctcgtgcg tgtggcggtc gactgtgacc tggcgctcct gcatgcactc gagcagcccg
     6721 gactgcgtct tcggcgaggc gcggttgacc tcgtcgacga gcaccacgtt ggcgaacacc
     6781 gggccgggcc ggaactcgaa gcggttctcg cgctggtcgt agacgtcggt gccgacgagg
     6841 tcggcgggga gcaggtcggc ggtgcactgc acgcgcgcgt ggtcggcgtc gatcgagcgg
     6901 gcgagcgccc gggcgagcgc cgtcttgccg acgccgggga agtcctcgat cagcacgtgg
     6961 ccgccggcca tcagcgccac catcacctcg cggacgacct cggccggcgc cttcacggcc
     7021 gtcccgacgt tgcgctcgat gcgcgccccg agctcggcca cctgccgcgg gtcgagctcg
     7081 ggcacggttg acataggcac caatcctagg gggcggtgtc atcggtcctg ctgacctatt
     7141 taggaacgcg gttgactttg atcgagcagt ccgtttaggg tggcgccagc cgcattgaag
     7201 tgagggagcg gcgtaactgg cgggaggggc ggttgaccgg ctcgtcgaca acagtcggga
     7261 ggggtcatga gccggagttg ggtgtcgtcg tcgtcgtcgt tgtccgcggg tgcgcggttg
     7321 ctgatctgcg cggcggcggt ggtggcgtcg ctgctcgcgc tcggcgcgac tcagaccgcc
     7381 ggccagggcg ccgcgcccga tcgggacggc cgcggcgcga gcggcgcggc gggacccacc
     7441 aagcccgacg gcggtggggg agtcgcctcc gtcgccggag acgagcttgc ccacctccgt
     7501 acgcgggact cgcgcacctt cgtccacgag tccggcagcc gagtcactca cctgtaccag
     7561 ggggcggtca acttccgcga cggcgccggc aactggcggc cgatcgacaa cgagctggtc
     7621 ccgtccgcgg ccgatcccga ctcgttccag aacgccgcta accggtatcg cctcagcctc
     7681 ccgcgcgatg tcggtcggga tcccgtccgg atgtcggcag gtggggactg ggtcgacttc
     7741 agcctgcgag acgccgccgg agcggcgacc gtcgatggat cggtcgcgac ctaccgggac
     7801 gtccgagagg ggatggcgtt ggcctacacg gccacggccg acggagtcaa ggaggagatc
     7861 cgcctggact cgctgtccgc cgcgcgcgac ctcgtcttcg acatgcgagt cggagcaggc
     7921 ctgacggcgg acatgcggtc ggacggcggc gtggacgtcc gcggcgagga cgggaggact
     7981 cggttcgtgg ttgcgccgcc gttcatgatc gacagcgcca agaacgctga gccctcctac
     8041 gccgtctcgt acgagctcga ccgcacggcg accgggtatg agctcgccgt tcggcccgac
     8101 cgccagtggc tggagagccc gaagcgacgc tggccggtcg tgatcgaccc gacgacgacg
     8161 atgccgaatc cggtgaatga ctgcttcatc accgagggca acccgaacac cgccaactgc
     8221 tacaacgccg acctcgaggt cggctacgac gctcagggcc acggcgcgca cgaccaccgc
     8281 tcgctgctgc gcttcccgat cggcgattcg atcccgcggg gagcggtcgt gctgtcggcg
     8341 gagctcgaca tgcggttgtt cgggcggacc acgtctaacc cggtccccgt ctccgtgcac
     8401 accgtgaagt ccagcttcga cgcgaacgac gaccgtccgt ggacctcctc cgtcacgtgg
     8461 aacacctgga acggctcgaa ctcctggagc gcacccggcg gggatttcga cgcgacaccc
     8521 ctggccacga cgacgatggg cggccagaca ggctggcagg agtggttcct cgatccgcat
     8581 cgcgtgcagg agctcgtcca gcgcagccgc accgacaacg gcttcctgct caagagcccg
     8641 ggcgccgccg gacccgccaa ctacctccag ttcgcgtccc aggactacga catcgccgac
     8701 gcgccgcacc tcgagatcgt ctgggacgag cggatcggcg agaaggcgga gtacacgttc
     8761 gagcgtcagc ggctctccga ccggatggag ctggcggtca acgtgggcgt cggcaacctg
     8821 atgctcacag caacggatct gcaaatcgcc ggcacgggga tcgaccaggc gcattcccgc
     8881 acgtggaacg ccatcacccg tcgcccctgg tcgcgcgcgg ggcagtggtg gaacgtcgac
     8941 tcggccgagg acgtccggct cgagaccttc gacgacggtt cggccgtctt cttcgggccc
     9001 accggtgtcc cgatcccctt ccacaagacc ggcgccgccc agtgggagcc gtcgcctggc
     9061 tacagggcca cgctcgcgac cgagggcgcc gggcacaagc tgacgtttcc cgagagcgga
     9121 acgatcctcc acttccgcgc ggacggtctg ctgaccgagc aggaggaccg caacggcaac
     9181 gcgatccagt tcgcatacac gaactggaat gcgctcgatg tggtccacga cacgcagggg
     9241 cgcgtgaccg actatcacta caacgccgag gggctgatcg aggagatcag cgatcccggc
     9301 gggcgcagcg tccactacga gtacgacgac gacatgctcg agagctacac cgacgccgag
     9361 gggaacacca cccactatga gtacgtcaac gacgaggact tggtcgaaat caccgatccg
     9421 cgtggaaacg agatcgagtt cgactacgac ggctcgcatc gcgtcgtgtc gatcacgagg
     9481 gtgaacgatc agccctatgt gggcgacggc ccgacctggg agttcgacta cgacaccggc
     9541 gacgttcgct gccccgttcg gaccgtcagc aacgtcatga ccgatccgcg cggcaagcag
     9601 accgtctact gccgggatcg catgttccgg gtcgtcaaga cgatcgatgc gaacggcaac
     9661 gagcgatcga gctcctacag cgcgaccaac cgcgtgctag gcacgaccgc tcccgggtcg
     9721 gcccagacca ccatgagcta cgacggccag gacaacctga cgtccgtcga cgagccagcg
     9781 ggcgagcaga gcagcttcga gtacaacagc ggcacgcatc cctatttccc gaccaagcac
     9841 acctcgccgc agggaaccgg cagcgtcttc gcctatgaca ccgccggcaa cacgacgacg
     9901 atcggcgact cgagctcgcc cgcctcgcag gtcgaggcga agctggagta caacggccag
     9961 acgggcggtt cctgcccgga cgaccccacg acgaagccgg ggactctgcg ttgcgcgatc
    10021 gacggcaagg gcaaccaaac gctctacggc tacgacgacg acggcaactt gacgtcggtt
    10081 acgcccgagt tgccgttggg cgataccacg atgacgtacg actcgctcag tcgcctggcc
    10141 accgtcgagg acggcaaggg ccagacgcgc agctacagct acgacgcgct cgaccgcgtc
    10201 actgagatcg actacggcgg cggcaacacc gtcagcttcg actacgacgc gaacgggaac
    10261 cgcatcgagc ggatcgactc cgtgcacggg acgtcgacat gggagtacga cgagctcaac
    10321 cgccgggtcg aggacgacct gccgtcgggc gtgaccgact acacctggga cgccgcgtcg
    10381 aacctgagct cgctgaccga cggcggcggg accgttagct atcgctacga cgacgtcaac
    10441 cgcctgatgg acctcgccga gccgggggga tcctgcacga cgcccgtttc gctgtgcacg
    10501 acgttcgggt acaccaaccg cgatcagcgc gagcggacga cgtatccgaa tggggtggag
    10561 cagacggtga ccttcgacag ctccgacaag ccgacgcggg tgcgcgcggc gaagggagcg
    10621 accgtgctga ccgacttcac ctacgactac gagatgtcgc cgactacgga gaccaagctg
    10681 cgccagtcgg tcaccgacaa ggacggcaac aagacgacct acagctacga cttcctcgac
    10741 cggctgacca gtgccgtgga gcgcaacagc tcgaacgccg tggtcgacga ccgctcctat
    10801 gactacgacg cagcgtccaa ccggacctcg caggtggtca acggtgctac cacgagctac
    10861 gcctacaacg ccgcaaacga gctctgctgg gttcacggca gtgcgtcgaa cgcagcttgc
    10921 aacgccgccc cgagcggagc gatcaccttc gggcacgacc tcaacgggaa catgaccacg
    10981 acgaacgcgg gcgtcggctt cgcctacaac gtccgtgatc agaccaccag cttcgactgg
    11041 cccggcgccc cgacgacgac gttccagtac gccggctcgg gtcagagcga gcggacggcg
    11101 gtcggcggga tcacgcagca gaacacgctg ctgggcctgt cccgcgaggg ctcgacgtct
    11161 tggacccgcg accccgacgg caccttgatc tcacagcgcg ccagcggcgg tgttcgccac
    11221 tactacctgt ccgacggtct cggctcgatc gtcgggctga ccgacagcac aggagctctg
    11281 acccgctcct acaagtacga cccctacggg ctcatccgtg ccaccactgg ctcaacaccc
    11341 aacccgttca agttcaccgg ccaataccac gaggcccaag gcaacctcta caagatcggc
    11401 gcccgctact acgccccgac cctaggccgc tggacgcagc aagacccgct cgatcaagta
    11461 ggcgacctac gcgacggaaa ccggtacgtc tacgccggag cagatcccat caaccggata
    11521 gaccctacgg gactgcttag ttttgacgag atcgtgcgtg aggtccaaga gacggctggc
    11581 gaggtccaac gtatctatga cgatatccaa tcactcggcc ggactcgcgg cggacgtgcc
    11641 ttagccaggt gcctacttgc cggcggggtg gtcacggccg tttccaagaa cccgggctgg
    11701 ggtcttgccg tctgcggtgg agtcactgtc gtgacgagag gtagatgaga ctgcccatca
    11761 agtacaaggt accggcatgg ataatcgcga tccttctatt gctgcctatc ctcctcgttg
    11821 acagccagaa cctgcgtggt ttgttggctg cgatcgtgat tcttggctgt ttcttgtatg
    11881 agagtcgctc tgtcgagcgc atatcacgtc gatcaataat ccgcggtcta tcctcactcg
    11941 caatcgtggt ggcggcagcc atggggactt gggcgctcat tgcgcttacg gacttggacg
    12001 cgggtttagc gtggctgtcg ggacttttgg ccggaatgag cgcgactaaa ctcatcgaga
    12061 ggactcccct acgttcgcgg cagccaggca atggggtgca atagtgtgtc gaacgctgct
    12121 tgcaacgcgg ctccgaacga cctttatggc ctcatccgcg cgaccaccgg ttcaacatcc
    12181 aacccgttca agctcactgg gcagtaaacg aggcccaagg caacctctac ggacggcgag
    12241 caagaacccg aagggggcaa tgaggactag gaaggctccc aacgcgatga gtcaaggcca
    12301 tatcacgagc ccgaggacga tggtaattgc gcatatgacg acgacggatc tgatttgggc
    12361 cggtcgtggt cgtggtcgct ggggatacgg tctagcagcg atccgcgcag gggtctcatc
    12421 gctcgtccga taagcgcgat tgctagcagg gtcctctgct gccacatccg aagtctgccc
    12481 ggacaggtcc aacggaaacg ttacgtagag cgtttgcctc gataccaccg gaacggcccg
    12541 gtgagccgcc gccacggtgc gtgtaggcat ccatctcggc gctgggtaga tcggaggcat
    12601 cggcaccgag caaccaggag gaacatgacg gaggaggagc ggcagaggat ggcccagctg
    12661 gaggatcgtg tgtgaatctt cgccgctgcc gcaggcgtta acgtcaccct ggacctccct
    12721 cagggcgtga tcgacgctgc tcgtcgagga gatcggatcg aggcggtgag gggatttcgg
    12781 gagttgggct tgggactcgc agcagcagcg cgtgccgcgg atgccgcggc cggtgaggaa
    12841 cgaggttccg ccgaccggcc aggcgatccc ctcgtgcgag tcgcgcgcgt cctcctgcca
    12901 ctcgccggcg ctctgttcgt gacgatgctg ctagccgagt caacaacgga tggcgccagc
    12961 gtgctgctcc tgattcctgc cggcttggcg atcgtggcgc ggggagtcct catgctcgtc
    13021 tacaccgacc gggtgctggt gccgctggcc cgccgggagg ctgccagtcc gtgggcaaag
    13081 ttcgggatga acactgagta ccgctacggg gcggctctac tagtggttat cggactgggg
    13141 tggatgctgg gcggagctgt ctatgggctc tgagcggccg cccgccaagc gccggcggac
    13201 gcaaacaccc gcgcggccct tctggctggc ggcctcgatc ttctggattg gtggtggcgt
    13261 ggcagtcatc gctggcaagg tgttgctcgg cgccgccctg ctcctcgtcg ccgcggcggc
    13321 actgctcatc ctgcgatcgc tccatcggcg tgccaagggt tcggtggaca gagatcacgc
    13381 gtcgatcctc gacatgctcg gtccctgatg gcgaagcgcg gcagtcttcc gcggcgtggc
    13441 aggcggaccg gcgaaacgga ttctcaacgc ccggcggacg ccgccccgcc tcgtgaggcg
    13501 aacaggtagc aagtggatga gggccgtgac aacccgaagg acctcgaaat cttcgaggtt
    13561 aggagacagc tcatgagtca tgccgctttg agcgctgtgc tggcagtcgc ctcgacggtc
    13621 gccgtgttgg tgatcttcga ctgggatgca agtgaggtag cggtctgggg cggtatcggc
    13681 gcggtggtct ggacggttgt tgctttcttc acatggactg ccgcgatgtt gcctcgaggc
    13741 gacctcctgc ccccaccacc tgacgttgtc ctcgggcgcc gcggggggct gatccaagat
    13801 gcgatccggt tggtcgccgt cgtgatggcg tgcgttggca tcgcatggct cgccaccgag
    13861 atggatgccg gcgccttctc gatccctggt cagttcattg gctacgcctg cgcctcgctc
    13921 gccggcgccc tttgggtcgc atggtgggag aggcggcatg gacaaacggt cgtggccagg
    13981 tacgagcgcg aggacgatga cgagctctat gcgacgcctc tggcaccaga cgctgatggc
    14041 tgacatgctc gctcaggtgt ggctgtgggt ggcagacgcc ttcgaggggc gcccggtcgc
    14101 cgcgctcttg acggcactgg catttgccgc tctaatcggc ggcgcggtct tcctagtcgg
    14161 tgcctagcgt gagtggctac tacgatcagc cacgagggca ccgacacgaa gattgctatg
    14221 gctgtgggag ggcgacgctc gcggctggct cgtgatggtg ctgactgtcg ttggcactgt
    14281 tgtggccgcc tggggtctgt cagagatggg ctcatgacgg tgatctagac atgaccactg
    14341 acgacctcaa acagctcgtc cgtgacgtcg agctgcctcg tcagaaggtc taccgcgccg
    14401 gggacgtcca gtacgttccg gcatggaggc tcgtggcggt cgcttcccga agctatgggc
    14461 tgggcgaaga tgccgagaag caggtcgacg ccttggtcgt ggagcttggg gggaaacgaa
    14521 tcgatgtggt cgacccatgg cgcgtgattc cagacctcat caagcgccta ctcggacgac
    14581 cgattcctcc acgcgaggat ctctacgaac tgccaacgac gatgctcgaa acgtgatgcc
    14641 gagagctctt gtcgctttcg tcaccgcctt gtctggcgcg gcgcttctgg tcggcgcata
    14701 tcgggccatc gctctggggg agcagaatcc ggcgcgtggc ctcctgatgt tcggcgggat
    14761 cgtcttcctc accaatgtgt ccatgtggat cgcgcgtcga cggggtgagg acacctggta
    14821 agcgcgcctc ggatcgtgcg ctagccggtc gagcgcgatc ggcggtcgag ccggatgaag
    14881 acggtggcca ttgcgaagac gatcaggccg caggcggcgc caccggcgag ggcctccggc
    14941 aggctagcgt cgccgaggaa gaaggcgatt gcggtaatca gcgggatggt gatcagggcg
    15001 gcgatgactg cgtctgggaa gacttgctcg ggtttccaat ggtcggtgcc gtagcggtcg
    15061 gcgtcccgcc taacgagggc cgcctggacc tcgagcagtg gccacaggaa ggcgaatggc
    15121 cagaagaacc tgaggtcgat ccagccagac aggaagaaga ctgccacgcc ccagggaagg
    15181 aggaggacga gcgagaggcc gaatactcgc caggggggag ttgcctccgc ggatctgtcg
    15241 tcgggagcgt cgcggccgtc ggcaaaccgg cgaaccgcca aggcgaacgc gagcagcaca
    15301 gcgaactgca cggtctcgga gtgcgtcagc agccacgacg ccgccagtgc ggccagcgcc
    15361 gcgacgagga ttgcccgcat agcccagctg ggagttgcgg ccttcatggc tttcaggtct
    15421 tggcgtcggc ggggagcggc tgtggctcgc cgattgcgcg accgtccgag cttgccgtcc
    15481 gagacccacc gttcgcgccg ccgtccgtct tggccagctc cgacggccac cagaacttgg
    15541 ggccgatctt gagggcgagg gcggggacga gcacggagcg gacgaggaag gtgtcgagca
    15601 ggacgccgaa ggcgacgacg aagccgatct cggtcaggaa gacgagcggc aggacggcga
    15661 ggacggagaa ggtcccggcg aggacgatgc cggcgcttgt gatcacgccg ccagtgacgg
    15721 cgagggcgcg gagaacgccg tcgcgggtgc cgtgcttgag cgtctcctcg cgcgcccggg
    15781 cgatcaggaa gatgttgtag tcgacgccca gtgcgacgag gaagacgaag gcgaacagcg
    15841 gcaggccggg gtcggagccg gggaagccga agaccatgtc gaaggcgaag tagcccacgc
    15901 cgagggcggc ggcgaagctg aggatcaccg tcgcgatcag gatcagaggc gcgacgacgg
    15961 ctcgcagcag gaggacgagg atcaggaaca cgacgacgag gacgatcggc ggtatcacga
    16021 tcgagtccca ggcggaggcc tcgcgcaggt cgaactcgac cgccgtcggc ccgccgacga
    16081 gcgtgtcggg tgcgacgtcg cggactgcgt cgcgaagcgg ctccacgatg tcgtaggcct
    16141 cggtggagta gggggtcggc tcgagcgtcg cctggaccag cacgccgccc tcgccctggg
    16201 cgaccggcgg ggtgacggac tcgacgccgg ggacctgctc ggcggcgcgc gtcacggcct
    16261 ccacccgccc gggatcggcg acgatgatgt cggtgggcgc cgccgagccg gcggcgaagc
    16321 tctcggcgag gatctcctga ccctggaccg actcgacctc ggtgcggtag ctgtcgttgc
    16381 tggtcaggtc ggtcgagaag aaggcgagac cgcagcagag cacgaacagg acggcgagcg
    16441 agccggccag cacgcggttg cgtccgcggg cgacccagtc cccgacccgc ttccaaaacc
    16501 cgtgcgtcgc gtcggcgagg tgctcgcggc ggtagcggcg cagctcgtac gggcggaaga
    16561 cgcgcttgtc gagcgggccg acgatcagcg acgggaagcg ccagtccgtg aaccagcgca
    16621 cgacgagcgc gacgatcagc ttgaccacca ggttgagcag caccagcggc agcaggacga
    16681 tcaccagcag gctggcgagg atcacgctgc ccagcgccgt gacgcgcggc ccctccgtga
    16741 tgttgcgcag cgaccacccc ggaacgccct cctccgtcgg cgcggtctcc ggcgtgtgcg
    16801 gcacgaacgg ccagaacgcg cgtcggccga agatcgtcag cagcgccggc agcagcgtca
    16861 gcatcgacag cgccgcgcag gcgacgccca tcgcgccgat cgggccgagc cccgacgtgc
    16921 cgttgacctt ggcgatcgtc agacagaaga gcgcggcgat gacggtcgcc gcggaggcga
    16981 agatcgctgg tccggccgag gcgagggcgg tctgcatcgc ctggtgcttg tcggcggtgt
    17041 gatgcagctc ctcgcggtag cgggcgacga tcaacagcgc atagtcggtc cccgcgccga
    17101 ggacgaggat cgacatgatc gagctcgact ggccgttgat cgtcacgccg agctcggaga
    17161 tcccgtaccc gaccgagcgc gaaagcatct cggcgaagac cacggctagc agcgggatca
    17221 ggaagaagat cggcgaccgg tagatcacga tcagcaggaa gatcacgagg ctgagcgcgg
    17281 ccagcagcag cgtgccgtcg atgctctcga agacctcgat cgcgtccgcc gcatagccgg
    17341 cgccgccggt gatcttcacc tcgaggccgc cgcccgggtc cgagacctgg tcgcgccact
    17401 cctggatcgg gtcgaggatc gtgtcggcct cgccgtcgcc cttcaggtac gcggtgacga
    17461 tcgccgcctt gccgtcgcgc gagcagatcg ggccgacgaa cggggcgtag ccctcgggct
    17521 gcccggcgac cggagtggtc gggccgccgc agcccgcggg ctggccgctc tgtgggctcg
    17581 cggcgccgcc ctgcgcgctg ccggtcgtct cggcgggcga ctgacctccg gcgccggccg
    17641 gcccgctctc gctgggcccg cgaccgccct gctgacc
//
LOCUS       DATABV010000200         5951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1992355, whole genome shotgun sequence.
ACCESSION   DATABV010000200 DATABV010000000
VERSION     DATABV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992355"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VHF88_09195"
     CDS             <1..278
                     /locus_tag="VHF88_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161980.1"
                     /translation="MGARVIVCDPHRAVVSGPSRLHGERMESPDIRAGMAMLIAALCA
                     EGSSEIGNVRQIDRGYERIEERLRALGARIERVPPRGVTPQGEPLPV"
     gene            312..1316
                     /gene="hisZ"
                     /locus_tag="VHF88_09200"
     CDS             312..1316
                     /gene="hisZ"
                     /locus_tag="VHF88_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX2161981.1"
                     /translation="MIHPIPAGTRDVLPDEMRELRAIDERLRSTFAEAGYGEVWTPMV
                     EYEEVFAAGAAPGQPGAAGGTGGASPPTAIRMFDDAGKVMALRSDMTVPIARVVATRF
                     AESERELRLCYSAHSYRTVGDRTGQQREFLQSGIELIGVPGPTGEAEVVELALGALAN
                     AGLARHRIGVGDGSLYRRLLIALEVPPDRHRELLVCLTRRDLVGLERRVDVLGLSRDA
                     RDLLVDLPELRGGPEVLERGDGVAADALARLRELYAALEARGLADRVILDLGLVRDLG
                     YYTGAVFELYDPAVGFALGGGGRYDDLIGRFGAPRPACGIALDVQRVHIAQAAEERQR
                     "
     gene            1313..1969
                     /gene="hisG"
                     /locus_tag="VHF88_09205"
     CDS             1313..1969
                     /gene="hisG"
                     /locus_tag="VHF88_09205"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX2161982.1"
                     /translation="MSATGLTIAVPRGALMGGTLDLLDGLGVDTAEVRSNDRKLLFEE
                     AGIVTMRPSDVPTYVEHGAADIGITGKDVLLESPERDVYELLDLGYGRCEMVVARRSG
                     DDALGESLRRLGRVRIATKYTRIAAEHFARTGRQVELVAVKGSVELAPLTGMVDGIVD
                     LTATGKTLAENGLEVVEDIVTCTARLIANPVAHKLKAAEIDAVVQALRAATARDGRPA
                     "
     gene            1966..3222
                     /gene="hisD"
                     /locus_tag="VHF88_09210"
     CDS             1966..3222
                     /gene="hisD"
                     /locus_tag="VHF88_09210"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX2161983.1"
                     /translation="MRSRSFGPDATAADIRGLTPDPRSVEAGVREILAEVGARGDAAV
                     AELTQRIDGVRIDPSRLRVEPATLEAAGASLEPQVADGLRAAIANVRAVAEAQLRGQV
                     AVELADGQVVDVEELPVRRAGIYAPAGRAAYPSSVVMAAATARAAGVEQVAVCAGPGP
                     DGEIHTAILAACALCGVDEVYRVGGAQAIGALAYGTETVAAVDVIAGPGGPWVQEAKR
                     QLVGTVGIDGIAGPSELVVVADGDADPEPLALDLLAQAEHGPDSLVVAIAPAAELLEA
                     IGAAVERLVPDRPSVQDTTLALVRSATAAAAVELADAIAPEHLQLVGPEPEALAGRVR
                     AAGCVFVGEAAATAFGDYVAGSNHILPTGGAARFQSAVSPATFRRRMARVSFPGDAAA
                     RLAARGAAVARAEGFPVHAESMERRA"
     gene            3219..3806
                     /gene="hisB"
                     /locus_tag="VHF88_09215"
     CDS             3219..3806
                     /gene="hisB"
                     /locus_tag="VHF88_09215"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX2161984.1"
                     /translation="MTRSADIARRTKETDVELSLELDGDGAGTRATGVGFFDHLLDAI
                     ARHGRIDLDVKVEGDLETGPHHTVEDTGLAFGRALDEALGDRSGIRRYGHAVVPMDEA
                     RASCALDVSGRPFAAFEADLPAEAIADFDSDLAEEFFRAVANTAKLTLHVTVERGVNT
                     HHMVEAAFKAFARALREAVAIDPDERGIPSTKGLL"
     gene            3803..4447
                     /gene="hisH"
                     /locus_tag="VHF88_09220"
     CDS             3803..4447
                     /gene="hisH"
                     /locus_tag="VHF88_09220"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX2161985.1"
                     /translation="MSGGPTVAILDYGMGNLRSAEKAFEHVGASAPITREHDVVRAAD
                     GVVLPGVGAFPKAMAAVRELGLDSLLRERHEAGVPILGICLGMQLLYEGSDEHEGAEG
                     IGLLPGRIEALDAPGLKVPHIGWNPVSWRRESEIADGLPEPCAFYHVHSYAKRETDSD
                     EVVGVASYGQEFASVVEAGDVYGTQFHPEKSGPDGLALLRNFVARCRAGARAAA"
     gene            4444..5187
                     /gene="hisA"
                     /locus_tag="VHF88_09225"
     CDS             4444..5187
                     /gene="hisA"
                     /locus_tag="VHF88_09225"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX2161986.1"
                     /translation="MILLPAVDIRDGKAVRLRQGRFDDETVYADDPLEAARSFVEAGA
                     RYLHVVDLDGARDGAPANLDHLERITAELEVPVQYGGGLRSLQSVRAALAAGAARVVI
                     GTAAYGDADFLQTALETWRQRILVAVDVRGGHVSVSGWTRETQMRPEELIARLQQQGA
                     TRFVYTNADRDGMLEGVDVEEVRRISAAVRGRFIMSGGIGSLADLEALRDLRLLNLAG
                     VISGKALYEGRFSVRDGRAALEAKPAPRP"
     gene            5190..>5951
                     /gene="hisF"
                     /locus_tag="VHF88_09230"
     CDS             5190..>5951
                     /gene="hisF"
                     /locus_tag="VHF88_09230"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX2161987.1"
                     /translation="MLLKRVIPCLDVDRGRVVKGTNFVDIRDAGDPVERAVRYQDECA
                     DEIVFLDITASHEKRDTVAALARRCADDVFIPFTIGGGIRSVADAQVVLDAGSDKVSV
                     NSAAVERPQLIGELAEVFGAQCVVLAIDARKRTVAGAGYEVLVHGGRTETGREAVEWA
                     REGVERGAGEILLTSMDRDGTEDGYELELTRAVADAVDVPVIASGGAGTLDHLVDAVE
                     RGGADAVLCASIFHYGKYRVRDAKEHLAAAGIPVRR"
BASE COUNT          772 a         2172 c         2233 g          774 t
ORIGIN      
        1 ccatgggcgc tcgggtgatc gtctgcgacc cgcaccgcgc ggtcgtcagc ggcccgagcc
       61 ggcttcacgg cgagcgcatg gagagcccgg acatccgcgc cggcatggcg atgctcatcg
      121 cggcgctgtg cgccgagggc agctcggaga tcggcaacgt gcgccagatc gaccgtggct
      181 acgagcggat cgaggagcgc ctgcgcgcgc tcggcgcgcg gatcgagcgt gtccccccgc
      241 gaggcgtcac cccgcagggt gagccgcttc ccgtctaggc tgcgcgtcac accgccgagg
      301 aggggacggt cgtgattcac ccgatacccg cgggcacgcg cgacgtgctg cccgacgaga
      361 tgcgcgagct gcgcgcgatc gacgagcgtc tgcgctcgac cttcgccgag gctggctacg
      421 gcgaggtctg gacgccgatg gtggagtacg aggaggtctt cgcggctggg gcggcccccg
      481 gccagccagg cgccgcgggg ggtacggggg gcgcgagccc cccgaccgca atccggatgt
      541 tcgacgacgc gggcaaggtc atggcgctgc gctcggacat gacggtgccg attgcgcgcg
      601 tcgtggccac ccgcttcgcg gagtccgagc gcgagctgcg gctctgctac tcggcgcact
      661 cctaccgaac ggtcggggac cgcaccggcc agcagcgcga gttcctgcag agcggcatcg
      721 agttgatcgg cgtgcccggg ccgaccggcg aggccgaggt ggtcgagctc gcgctcggcg
      781 cgctcgcgaa cgccggcctg gcgcggcacc ggatcggcgt cggcgacggg tcgctctacc
      841 gccggctgct catcgcgctc gaggtcccgc ccgaccgtca tcgcgagctg ctcgtctgcc
      901 tgacacggcg cgatctcgtc ggcctcgagc ggcgcgtcga cgtgctcggc ctgtcgcgcg
      961 acgcgcgcga cctgctcgtc gacctgcccg agctccgtgg cggtcccgag gtgctcgagc
     1021 gcggcgacgg tgttgccgcc gacgcgctcg cccggctgcg cgagctctac gcggcgctgg
     1081 aggcgcgcgg cctcgccgac cgggtgatcc tcgacctcgg cctggtgcgc gacctcggct
     1141 actacaccgg tgcggtcttc gagctctacg acccggccgt cggcttcgcg ctcggcggcg
     1201 gcggacgcta cgacgacctg atcggccgct tcggtgcgcc gcgaccggct tgtgggatcg
     1261 cgctcgacgt gcagcgggtc cacatcgcac aggcggccga ggagcgccag cgatgagcgc
     1321 gaccggtctg acgatcgcgg tgccgcgcgg cgccctgatg ggcggaacgc tcgatctgct
     1381 cgacggcctc ggcgtcgaca ccgccgaagt gcgctccaac gaccgcaagc tgctgttcga
     1441 ggaggccggc atcgtcacga tgcggccgag cgacgtgccg acctacgtcg agcacggcgc
     1501 ggccgacatc ggcataaccg gcaaggacgt cctgctcgag agcccggagc gcgacgtcta
     1561 cgagctgctc gacctcggct acgggcgctg cgagatggtc gtcgcgcggc gcagcggcga
     1621 cgacgcgctg ggcgaatcgc tgcgccggct cggccgcgtg cggatcgcga cgaagtacac
     1681 gcggatcgcc gcagagcact tcgcgcgcac cggccgccag gtcgagctgg tcgcggtcaa
     1741 gggatcggtc gagctggccc cgcttaccgg gatggtcgac ggcatcgtcg acctgaccgc
     1801 caccggcaag acgctcgccg agaacggcct cgaggtggtc gaggacatcg tcacctgcac
     1861 ggcgcggctg atcgccaacc cggtcgcgca caagctcaag gcagccgaga tcgatgccgt
     1921 cgtgcaggcg ctgcgtgcag cgacggcgcg cgacgggcgg ccggcgtgag gtcgcgctcc
     1981 ttcggccccg acgcgacggc ggccgacatc cgtggcctga ctccggatcc ccgcagcgtc
     2041 gaggctggcg tgcgcgagat cctcgcggag gtcggcgccc gtggcgacgc ggcggtcgcc
     2101 gagctgacgc agcggatcga cggtgtccgc atcgacccgt cccggctgcg cgtcgagccg
     2161 gcgaccctgg aggcggccgg cgcgtcgctc gagccgcagg ttgccgacgg gctgcgcgcc
     2221 gcgatagcga acgtgcgcgc ggtcgccgag gcgcagctgc gcggccaggt cgccgtcgag
     2281 ctcgccgacg gccaggtcgt cgacgtcgag gagctgccgg tccggcgcgc cgggatctac
     2341 gcgccggccg gtcgcgcggc ctacccgtcg tcggtcgtca tggcggccgc gacggcgcgg
     2401 gcggccggcg tcgagcaggt tgcggtctgc gccggtcccg ggccggacgg cgagatccac
     2461 acggcgatcc tcgccgcgtg cgcgctctgc ggcgtcgacg aggtctaccg cgtcggcggc
     2521 gcgcaggcga tcggggcgct cgcctatggg accgagacgg tcgccgcggt cgacgtgatc
     2581 gcgggccccg gcggtccgtg ggtccaggag gcgaagcggc agttggtcgg cacggtcggg
     2641 atcgacggga tcgccggtcc gagcgagctg gtggtcgtcg ccgacggcga cgccgaccca
     2701 gagccgctcg ccctcgacct gctcgcgcag gccgagcacg gtcccgacag cctcgtcgtc
     2761 gcaatcgcgc cggctgccga gctgctcgag gcgatcggcg cggcggtcga gcgcctcgtg
     2821 cccgatcggc cgtcggtgca ggacacgacg ctggcgctcg ttcgcagcgc gaccgccgcc
     2881 gccgcggtgg agctcgccga cgcgatcgcc cccgagcacc tgcagctcgt cgggccggag
     2941 ccggaggcgc tcgccggccg ggtccgcgcc gcgggctgcg tgttcgtcgg cgaggccgcc
     3001 gcgacggcct tcggcgacta cgtcgccggc tcgaaccaca tcctcccgac gggcggcgcc
     3061 gcgcgcttcc agagcgcggt ctcgccggct acgtttcggc gccgaatggc tcgcgtatcc
     3121 ttccccggcg acgccgccgc ccgcctggcc gctcgcggtg cggcggtcgc ccgcgccgag
     3181 ggtttcccag tccacgccga gtccatggag cgccgcgctt gacccgtagc gcagacatcg
     3241 cacgccgcac caaggagacc gacgtcgagc tgtcgctcga gctcgacggc gacggggcgg
     3301 gcacgcgcgc gaccggcgtc ggcttcttcg accacctgct cgacgcgatc gctcgccacg
     3361 gccggatcga cctcgacgtc aaggtcgagg gcgacctcga gaccggaccc caccacaccg
     3421 tcgaggacac cggcctggcg ttcggccgag ccctcgacga ggcgctcggc gaccgcagcg
     3481 ggatccgccg ctacggccac gcggtcgtgc cgatggacga ggcgcgcgcc agctgcgcac
     3541 tcgacgtctc cggccgcccg ttcgccgcct tcgaggccga cctgcctgcc gaggcgattg
     3601 cggacttcga ctccgacctg gccgaggagt tcttccgcgc ggtcgccaac acggcgaagc
     3661 tgacgctgca cgtgaccgtc gagcgcggcg tcaacaccca ccacatggtc gaggccgcct
     3721 tcaaggcgtt cgcgcgcgcg ctgcgcgagg cggtcgcgat cgaccccgac gagcgcggca
     3781 tcccgtcgac gaaaggcctg ctgtgagcgg cgggcccacg gtggcgatcc tcgactacgg
     3841 gatgggcaac ctgcgctcgg ccgagaaggc cttcgagcac gtcggcgcga gcgcgccgat
     3901 cacgcgcgag cacgacgtcg tgcgggcggc ggacggagtc gtcctgccgg gagtcggcgc
     3961 ctttccgaag gcgatggcgg cggtacgcga gcttgggctc gactcgctgc tgcgcgagcg
     4021 ccacgaggca ggggtgccga tcctcggcat ctgcctcggc atgcagctgc tctacgaggg
     4081 cagcgacgag cacgaggggg cagaggggat cggcctgctg cccgggcgga tcgaggcgct
     4141 cgacgcgccg gggctgaagg tgccgcacat cggctggaac ccggtctcct ggcggcgcga
     4201 gagcgagatc gccgacgggc tgccggagcc ctgcgccttc taccacgtgc actcctacgc
     4261 gaagcgcgag accgacagcg acgaggtcgt cggggtcgcg agctatggcc aggagttcgc
     4321 gagcgtcgtc gaggccggcg acgtctacgg gacccagttc caccccgaga agtccgggcc
     4381 ggacgggctc gcgctgctgc gcaacttcgt cgcgcgctgc cgggcgggag cgcgggcagc
     4441 ggcatgatcc tcctgccggc ggtcgacatc cgcgacggca aggcggtccg actgcgccag
     4501 ggccgcttcg acgacgagac ggtctacgcc gacgacccgc tcgaggcggc gcggtcgttc
     4561 gtcgaggcgg gcgcgcgcta cctgcacgtc gtcgacctcg acggcgcgcg cgacggcgcg
     4621 cctgccaacc tcgaccacct cgagcggatc accgccgagc tcgaagtgcc ggtccagtac
     4681 ggcggcggcc tgcgctcgct gcagtcggtc cgcgctgcgc tggcggcggg cgctgcacgc
     4741 gtcgtgatcg ggacggccgc ctacggcgat gccgacttcc tccagaccgc gctcgagacc
     4801 tggcgccagc ggatcctcgt ggcggtcgac gtccgcggcg gccacgtgtc ggtctccggc
     4861 tggacgcgcg agacgcagat gcgccccgag gagctgatcg ctcgcctgca gcagcagggc
     4921 gcgacgcgct tcgtctacac caacgccgac cgcgacggga tgctcgaggg agtcgacgtc
     4981 gaggaggtac ggcggatctc cgcggccgtg cgcggccgct tcatcatgtc gggcgggatc
     5041 ggctcgctcg ccgacctcga ggcgctgcgc gacctgcgcc tgctcaacct cgccggcgtg
     5101 atctcgggca aggcgctcta cgagggccgc ttcagcgtcc gcgacgggcg tgcggcgctc
     5161 gaggccaagc cggcgccgcg tccctagccg tgctgctcaa gcgcgtcatc ccgtgcctcg
     5221 acgtcgaccg cggacgggtc gtcaagggca ccaacttcgt cgacatccgc gacgccgggg
     5281 accctgtcga gcgcgccgtc cgctaccagg acgagtgcgc cgacgagatc gtcttcctcg
     5341 acatcaccgc ttcgcacgag aagcgcgaca cggtcgccgc gctggcccgg cgctgcgccg
     5401 acgacgtctt catcccgttc acgatcggcg gcgggatccg ctccgtcgca gacgcccagg
     5461 tggtgctcga cgccggctcc gacaaggtgt cggtcaactc ggcggcggtc gagcggccgc
     5521 agctgatcgg cgagctcgcg gaggtcttcg gcgcccagtg cgtcgtgctg gcgatcgacg
     5581 cgcgcaagcg gaccgtcgcg ggcgccggct acgaggtgct ggtccacgga ggccggaccg
     5641 agaccggccg cgaggcggtc gagtgggcgc gcgagggcgt cgagcgcggc gcgggggaga
     5701 tcctcttgac cagcatggac cgcgacggca ccgaggacgg ctacgagctc gagctcaccc
     5761 gcgcggtcgc cgacgccgtc gacgtccccg tcatcgcctc gggcggcgcc gggacgctcg
     5821 accacctcgt cgacgccgtc gagcggggcg gcgccgacgc cgtgctctgc gcctcgatct
     5881 tccactacgg gaagtaccgg gtgcgcgacg ccaaggagca cctggcggcc gccgggatcc
     5941 cggtccgccg c
//
LOCUS       DATABV010000201         5637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2475570, whole genome shotgun sequence.
ACCESSION   DATABV010000201 DATABV010000000
VERSION     DATABV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475570"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="purB"
                     /locus_tag="VHF88_09235"
     CDS             complement(<1..886)
                     /gene="purB"
                     /locus_tag="VHF88_09235"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2161988.1"
                     /translation="MIERYTRPEMGAVWSEQAKLDGWLAVELAVVDALAEAGAVPADD
                     AAAVRERASFTVEAVKEREQVTDHDVAAFVDVVAASVGEQGRWVHHGLTSSDVLDTAL
                     GLQIARAGAILVEGARGYRDALVARAREHAATLCVGRTHGIHAEPMTFGVKLAGFAFE
                     ADRNLRRLERAVEQASVGALSGAVGTYAANGPELETAVLERIGLAREDVSTQVVARDR
                     HAQLLNAIALAGAGLERFATEVRHLQRTEVREVEEPFRAGQKGSSAMPHKKNPIVSER
                     ICGIARLLRGYAQVGLEDV"
     gene            complement(883..1365)
                     /gene="purE"
                     /locus_tag="VHF88_09240"
     CDS             complement(883..1365)
                     /gene="purE"
                     /locus_tag="VHF88_09240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX2161989.1"
                     /translation="MTNDPEQSREPVTETEVEHQFDDLAVDAPRVGIVMGSKSDMPVM
                     EKAGKELEDRGIRYEIRVMSAHRDPDVVAEYAKNARMRGLRVVIAGAGLAAALPGVVA
                     AHTDLPVIGVPLTSKTSVAGGLDAMLAIAQMPPGVPVACVGVDGAKNAAVLAARILDA
                     "
     gene            complement(1362..2651)
                     /gene="purD"
                     /locus_tag="VHF88_09245"
     CDS             complement(1362..2651)
                     /gene="purD"
                     /locus_tag="VHF88_09245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX2161990.1"
                     /translation="MPRVLVIGGGGREHALVRRLLRSPQHPDLLCAPGNAGIARDKVE
                     CLDIAVDDVDGLVAAAREREVDLVVVGPEAPLVAGVVDALGEAGIRAFGPTAAAARLE
                     GSKRYAKELMAAVGVPTADYAVLTSRSEALERLAEAAYPLVLKADTLAAGKGVVICQD
                     EEQARAAVEELFTQQRFGKTDVVMEEFLAGEELSLLALCDGERAVPMAPAQDYKRIYD
                     GDEGPNTGGMGSYSPVPGIDREHAEALALAVHQPVVDELRRRGTPFHGVLYAGLMMTS
                     DGPKVLEYNVRFGDPETQAVLTRLCSDLLELLDAATRPGGLAGVEIEWSPDWAVTVVL
                     ASAGYPASSSSGDEISGLRAVDSIEVELLHAGTAERDGRIVTAGGRVLNVTGIGSTPA
                     EAREHAYRVAERIQFDGKQMRHDIAERAVARVEEVAG"
     gene            complement(2653..2934)
                     /locus_tag="VHF88_09250"
     CDS             complement(2653..2934)
                     /locus_tag="VHF88_09250"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX2161991.1"
                     /translation="MPTFVLLSTLTPEGVQTIKNNPQRILEVNREIEQLGAEVKAQWA
                     VLGRFDFVNVVEAPDEATMARVSLELGSRGTARYESLTAIPVDDFIASL"
     gene            3015..3683
                     /locus_tag="VHF88_09255"
     CDS             3015..3683
                     /locus_tag="VHF88_09255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2161992.1"
                     /translation="MAGRIYDATWGRAFALGYDFFFQRAERAGLRELRRRALAGASGK
                     TLEIGAGTGLNHDLYPPAVTELTLTEPFEPMARQLREKASALTVPVEVIETPAEALPF
                     PDDSFDTAVLTLVLCTVPEPDRALAEIARVLKPGGRFLFLEHVRAPDPGLARWQDRLA
                     GPWRAFGHGCNCNRDTLATIERSELEVERAERGEFPKMPPLVKPMLIGAAIVGSPPRT
                     ESRA"
     gene            3680..4336
                     /locus_tag="VHF88_09260"
     CDS             3680..4336
                     /locus_tag="VHF88_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161993.1"
                     /translation="MKLRSAVLITVTVAALAGPATALAATLSVDKACYGAGERVQFSG
                     GGYTPNGAVALSVSGRQLGVGSANAVGEFTANLAAPEIDGKRRTDIFTATDQANLALT
                     ASVPVLLTSLNVAVKPRNGDPSKPKRIVARGFTKGRTLYAHVRRGKVKRNLRVGRLKG
                     PCGTLSVKRRLFPPGAKPGLYKVQFDARRRYSASTTPQMAFMVTVFQTVRPARASAAG
                     "
     gene            complement(4347..4709)
                     /locus_tag="VHF88_09265"
     CDS             complement(4347..4709)
                     /locus_tag="VHF88_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161994.1"
                     /translation="MGKRARKRQREVGGAPSTSTSSYTDDAGNVLTLRDELSAGTLRG
                     LDKLDAKPAASAEDRWQRRVEYLFERLAVRWEIAGLPLEGQKELLGRYRMASSEERRA
                     VRAALAGHLRARYPDVDV"
     gene            complement(4747..4965)
                     /locus_tag="VHF88_09270"
     CDS             complement(4747..4965)
                     /locus_tag="VHF88_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2161995.1"
                     /translation="MSETERPGGPGDEEHEVTDKPYPTPDDVPRVGELGDDPDEGGDE
                     EQGPEVDQLDEDPAYNPDDPGLKGLKGG"
     gene            complement(5003..>5637)
                     /locus_tag="VHF88_09275"
     CDS             complement(5003..>5637)
                     /locus_tag="VHF88_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2161996.1"
                     /translation="EKHAVSRLVGAPPGYVGYDEGGQLTEAVRRRPYAVVLLDEIEKA
                     HADVFNILLQVMDDGRLTDGQGRTVDFTNAVLIMTSNVAGGLEGVQATFKPEFVNRLD
                     EIVQFESLTRAQIGEIVELAVSGLARRLSERGIEIELTDDAKTLIGNLGYDPAYGARP
                     LRRVVQRQLVDKLALKLLEGEFAPGDTVRVDAADGELTFTRAETPAAVGA"
BASE COUNT          748 a         2099 c         1980 g          810 t
ORIGIN      
        1 cgacgtcctc gagccccacc tgcgcgtagc cgcgcaggag cctggcgatg ccgcagatcc
       61 gctcggacac gatcgggttc ttcttgtgcg gcatcgccga cgagcccttc tgccccgcgc
      121 ggaacggctc ctcgacctcg cgcacctccg tgcgctgcag atgacggacc tccgtggcga
      181 accgctcgag gcctgcgccc gcgagggcga tcgcgttcag gagctgcgcg tggcggtcgc
      241 gcgcgacgac ctgggtcgag acgtcctcgc gcgcgaggcc gatccgctcg agcaccgccg
      301 tctcgagctc cggcccgttg gcggcgtagg tgccgacggc gcccgagagc gcgccgaccg
      361 acgcctgctc gacggcgcgc tcgagccggc gcaggttgcg gtcggcctcg aaggcgaagc
      421 cggcgagctt gacgccgaac gtcatcggct cggcgtggat gccgtgcgtg cgcccgacgc
      481 agagcgtggc ggcgtgctcg cgcgcccgcg cgaccagcgc gtcgcggtag ccgcgcgcgc
      541 cctcgacgag aatcgccccg gcacgggcga tctgcaggcc gagcgccgtg tcgagtacgt
      601 cagaggaggt cagcccgtgg tggacccagc ggccctgctc gccgaccgac gccgcgacca
      661 cgtcgacgaa cgcggcgacg tcgtggtcgg tgacctgctc gcgctccttg accgcctcga
      721 ccgtgaagct cgcccgctcg cgtaccgccg cggcgtcgtc cgccggcacc gcccccgcct
      781 cggcaagcgc gtcgacaacc gcgagctcga ccgccagcca gccgtcgagc ttcgcctgct
      841 cggaccacac cgcgcccatc tccgggcgcg tgtagcgctc gatcacgcgt cgaggatccg
      901 cgccgcgagc acggcggcgt tcttggcgcc gtcgacgcca acgcacgcga ccggcactcc
      961 gggcggcatc tgggcgatcg cgagcatcgc gtcgagcccg ccggcgaccg aggtcttcga
     1021 ggtcagcggc acgccgatca ccggcaggtc ggtgtgcgcc gcgaccacgc cgggaagcgc
     1081 cgccgccagg ccggcgccgg cgatcaccac gcgcaggccg cgcatgcgcg cgttcttcgc
     1141 gtactcggcg accacgtcgg ggtcgcggtg ggcgctcatc acgcggatct cgtagcggat
     1201 cccgcggtcc tcgagctcct tgcccgcctt ctccatcacc ggcatgtccg acttcgagcc
     1261 catcacgatc ccgacgcgcg gcgcgtcgac cgcgaggtcg tcgaactggt gctcgacctc
     1321 ggtctcggtg accggctcgc ggctctgctc tggatcgttc gtcatcccgc cacctcctct
     1381 acgcgcgcga ccgcccgctc ggcgatgtca tggcgcatct gcttgccgtc gaattggatc
     1441 cgctccgcga cccggtaggc gtgctcccgg gcctcggcgg gcgtggagcc tatcccggtc
     1501 acgttcagga cccgtccgcc ggcggtcacg atccgcccgt cgcgctcggc cgtgccggcg
     1561 tgcagcagtt cgacctcgat cgagtcgacg gcgcgcagcc cgctgatctc gtcgcccgac
     1621 gacgagctgg cggggtagcc ggcggacgcg agcacgacgg tcaccgccca gtcgggcgac
     1681 cactcgatct cgacgcccgc aaggccgccg ggacgcgtcg cggcgtcgag cagctcgagc
     1741 aggtcggagc acagccgggt cagcaccgcc tgggtctcgg ggtcgccgaa gcggacgttg
     1801 tactcgagca ccttcggccc gtcggaggtc atcatcaggc cggcgtagag gacgccgtgg
     1861 aagggcgtgc cgcgacggcg cagctcgtcg acgaccggct ggtgaacggc gagcgcgagc
     1921 gcctcggcgt gctctcgatc gatgcccggg accggcgagt agctgcccat gccaccggtg
     1981 ttcggcccct cgtcgccgtc gtagatccgc ttgtagtcct gggccggggc catcggaacc
     2041 gcacgctcgc cgtcgcagag cgcgagcagc gagagctcct cgcccgcgag gaactcctcc
     2101 atcacgacgt cggtcttgcc gaaccgctgt tgggtgaaga gctcctcgac cgcggcgcgt
     2161 gcctgctcct cgtcctggca gatcacgacc ccctttccag cggcgagcgt gtcggccttc
     2221 aggacgaggg ggtaggccgc ctcggcgagc cgctcgagcg cctcggagcg ggacgtcagc
     2281 acggcatagt cggcggtggg cacgccgacg gccgccatca gctccttggc gtagcgcttc
     2341 gagccctcaa gccgggccgc agcggcggtc ggcccgaacg ctcggatgcc ggcttcgccg
     2401 agcgcgtcga cgacgcccgc gaccagcggc gcctccggtc cgacgacgac gaggtcgacc
     2461 tcgcgctcgc gcgccgccgc gacgagcccg tcgacgtcgt cgacggcgat gtcgaggcac
     2521 tcgaccttgt ccctggcgat gccggcgttg cccggcgcgc acagcaggtc cgggtgctgc
     2581 gggctgcgca gcagccgcct gaccagggcg tgctcgcggc cgccgccgcc gatcaccagg
     2641 acgcgcggca cgtcagagcg aggcgatgaa gtcgtcgacc gggatcgccg tcagcgactc
     2701 gtagcgagcg gttccgcgcg agccgagctc gagcgagacg cgcgccatcg tcgcctcgtc
     2761 gggagcctcg acgacgttga cgaagtcgaa gcggccgagc accgcccact gcgccttgac
     2821 ctcggcgccg agctgctcga tctcgcggtt gacctcgagg atccgctgcg ggttgttctt
     2881 gatcgtctgg accccctcgg gggtcagggt gctgagcagt acgaaggtgg gcaaggcatt
     2941 cctttcgaat cgacgccgcc acagcggttt ctcgcagttt tcccgatccg gcgcaaggcc
     3001 ggtagcctcg gccaatggcc ggccggatct acgacgccac ctggggtagg gcgttcgccc
     3061 tcggctacga cttcttcttc cagcgcgcgg agcgcgccgg gctgcgcgag ctgcgccggc
     3121 gggcgctcgc cggcgcgtcc gggaagacgc tcgagatcgg cgccgggacg gggctcaacc
     3181 atgacctcta tccgccggcc gtgaccgagc tgacactgac cgagccattc gagccgatgg
     3241 cgcgccagct gcgcgagaag gccagcgcgc tgacggttcc cgtcgaggtg atcgagacgc
     3301 cggccgaggc cctgccgttc cccgacgaca gcttcgacac ggccgtcctc accctcgtcc
     3361 tctgcacggt gcccgagccc gaccgcgcac ttgccgagat cgcgcgcgtc ctgaagcccg
     3421 gcggtcgctt cctattcctc gagcacgtcc gcgcgccgga ccccggcctg gcgcgttggc
     3481 aggaccggct cgccgggcct tggcgcgcct tcggccacgg ctgcaactgc aaccgcgaca
     3541 cgctcgcgac gatcgagcgc tcagagctgg aggtcgagcg cgctgagcgc ggcgagttcc
     3601 cgaagatgcc gccgctcgtc aagccgatgt tgatcggcgc ggctatcgtc ggctcaccgc
     3661 cccgaaccga gagccgagct tgaagctgcg cagcgccgtc ctcatcaccg tcaccgtcgc
     3721 cgcgctcgca ggtccggcga ccgcattggc cgccacgctc agcgtcgaca aggcctgtta
     3781 cggcgcgggc gagcgcgtgc aattctccgg cggcggctac accccgaacg gagcggtggc
     3841 gctcagcgtc agcggacgtc agctaggggt cgggagcgcc aacgcggtcg gtgagttcac
     3901 cgccaacctc gccgcccccg agatcgacgg caagcggcgg accgacatct tcaccgccac
     3961 cgatcaggcc aaccttgcgc tgaccgccag cgtgccggtg ctgctaacgt cgctaaacgt
     4021 cgccgtcaag ccgcgcaacg gcgacccgag caagccgaag cgaatcgtcg cgcgcggctt
     4081 caccaagggc cggacgctgt atgcgcacgt gcggcggggc aaggtcaagc gcaacctgcg
     4141 ggtcggccgg ctgaaggggc cgtgcggaac gctgagcgtc aagcgaaggc tgttcccacc
     4201 cggcgcgaag ccgggtctct acaaggtgca gttcgacgcg cgccgcaggt actccgcgag
     4261 cacgaccccg cagatggcgt tcatggtcac ggtcttccaa acggtccggc ccgcccgggc
     4321 cagcgcggcc ggctgatccc agtcccctag acgtcgacgt cggggtagcg cgcacgcaga
     4381 tgccccgcaa gcgcggcgcg gaccgcgcgc cgctcctccg acgacgccat ccggtagcgg
     4441 ccgagcagct ccttctgccc ctcgagcggc agtcccgcga tctcccagcg gacggccagc
     4501 cgctcgaaga ggtactcgac gcgccgctgc cagcggtcct cggcgctggc ggcgggcttg
     4561 gcgtcgagct tgtcgaggcc gcggagagtg ccggcgctca gctcgtcgcg cagcgtgagg
     4621 acgttgccgg cgtcgtcggt gtaggagctg gtcgaggtgg acggcgcgcc gccgacctcg
     4681 cgctgccgct tgcgtgcgcg cttgcccatc gccgcagcgt agaacgcggc gagcgggcgg
     4741 cgcgggtcag ccgcccttga ggcccttcag tcccggatcg tcggggttgt aggcggggtc
     4801 ctcgtcgagc tggtcgacct cggggccctg ctcctcgtcg ccgccctcgt cgggatcgtc
     4861 gcccagctcg ccgacgcgcg ggacgtcgtc gggcgtcggg tacggcttgt cggtgacctc
     4921 gtgctcctcg tcgccgggtc cgcccggccg ctctgtctcg ctcatggggc ggaggtaccc
     4981 gtcgccctgg gggccaaacc ggctaggcgc cgacggcggc cggcgtctcg gcgcgtgtga
     5041 aggtcagctc gccgtcggcc gcgtcgacgc gcaccgtgtc gcccggcgcg aactcgccct
     5101 cgagcagctt cagcgccagc ttgtcgacca gctgcctctg cacgacgcgg cgcagcggcc
     5161 gggcgccgta ggccgggtcg tagccgaggt tgccgatcag cgtcttcgcg tcgtcggtca
     5221 gctcgatctc gatcccgcgc tcgctcagcc ggcgcgccag cccgctgacc gccagctcga
     5281 cgatctcgcc gatctgcgcc cgcgtcagcg actcgaactg gacgatctcg tcgaggcggt
     5341 tgacgaactc gggcttgaag gtcgcctgga cgccctcgag cccgccggcg acgttcgagg
     5401 tcatgatcaa cacggcgttg gtgaagtcga ccgtgcggcc ctggccgtcg gtcaggcggc
     5461 cgtcgtccat cacctggagc aggatgttga agacgtcggc gtgggccttc tcgatctcgt
     5521 cgagcagcac gaccgcgtag ggccgccgcc gcaccgcctc ggtcagctgg ccgccctcgt
     5581 cgtagccgac gtagccgggc ggggcgccga cgagccgcga caccgcgtgc ttctcca
//
LOCUS       DATABV010000202         8403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1593348, whole genome shotgun sequence.
ACCESSION   DATABV010000202 DATABV010000000
VERSION     DATABV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593348"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VHF88_09280"
     CDS             <1..258
                     /locus_tag="VHF88_09280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX2161997.1"
                     /translation="LAVALVSFGFGAASNVLSRQVEARADAFALELTDEPGAFVALER
                     SLARRNLGDPDPPRLLHGLFGTHPTTLERIGYGVAFGRSDR"
     gene            298..370
                     /locus_tag="VHF88_09285"
     tRNA            298..370
                     /locus_tag="VHF88_09285"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:331..333,aa:Gly,seq:ccc)
     gene            410..961
                     /locus_tag="VHF88_09290"
     CDS             410..961
                     /locus_tag="VHF88_09290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2161998.1"
                     /translation="MKRRSPFAVAAVLGVVALLGLLTYGLFASEPDRGMDDALARGET
                     VAAPGFELERLSGGGRESLRDYRGKVVVLNAWASWCEPCREESPLLQRWHERLSRDGR
                     GTVLGIDALDVDSDARAFVREYRLTYPMLRDPEAESLLDYGVIGYPETFVIDERGNIV
                     ALRRGPVDEQWMRDNVAPLLERS"
     gene            958..1419
                     /locus_tag="VHF88_09295"
     CDS             958..1419
                     /locus_tag="VHF88_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX2161999.1"
                     /translation="MKRALSLAAALMLLLLPGGAVAADCPKTSLAAVEADVMCPVCGT
                     PLGLATEAPQANRQRALIQRLVDRCRSKEEIEEVLVAQYGPGVLALPETDGFQLSAYL
                     VPALIVLLAAGGIALAARRWRGGGGRPATAGPAEADVASKRLDDDLERYEL"
     gene            1416..2165
                     /locus_tag="VHF88_09300"
     CDS             1416..2165
                     /locus_tag="VHF88_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2162000.1"
                     /translation="MIGADAVDTTVVAAFAVGFISFVSPCVLPLVPGYLSTISGVSFS
                     DIEAGRSRREVLWPALLFCLSFSLMFVALGMTATGLGQTLQDHRQLLREISGIVLMLL
                     GAFFIATLFISRLNREWRPEALLSHAGTGGPIVAGLAFAIAWLPCTGPTLGAVLTAAS
                     NESTVGQGGVLLAFYALGLAVPFVLSALAFSHVSGVFRFFRNHYAAITLISGAILIGM
                     GYLLFAGELTRLNAEALDLMDDLGINIFSDL"
     gene            complement(2172..3797)
                     /gene="argS"
                     /locus_tag="VHF88_09305"
     CDS             complement(2172..3797)
                     /gene="argS"
                     /locus_tag="VHF88_09305"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX2162001.1"
                     /translation="MTDPVKDLSEAVIAAAESLGGEPAKAPTLERPPQADLGDYSTNA
                     AMMLAGALKQPPRAIAERLGEALEGALGDGVERVEVAGPGFLNLFMADDWLKSAAAAA
                     LGDGDEFGRSVVEAPERVNLEFVSANPTGPITVAAGRHAAYGDSLARVLELAGSEVDR
                     EYYVNDYGGQVQRFGASIRARALGEAPPEDGYQGEYVKEVAAGIEGAGELDVEELAKR
                     GVAIMLEGVEATLRRFRVEMDRFSSERELQEGGAVEAAIERLREQGHVYESEGATWLR
                     STEFGDDKDRVLRRSNGEKTYFAADIAYHEDKRGRGYDHVVDVLGADHHGYLGRMRAA
                     WQALGGDPQRLEQVTMQLVNLLEDGQRAQMSKRGGQFVTLDELVDDIGVDATRWFLVQ
                     RSPDTALDLDLDLARQRSQDNPVYYAQYAHARIASILRRAAEGQVAAAVAGDLAVSRE
                     VLHPSARDLVKKLLELPEEVRIAADRRAPHRMAVYVTEVAQSFSAFYRDCKVIGAAEE
                     GGDEDLRLALCELTRRVIARSLDLLGVEAPDEM"
     gene            complement(3946..4353)
                     /locus_tag="VHF88_09310"
     CDS             complement(3946..4353)
                     /locus_tag="VHF88_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX2162002.1"
                     /translation="MRILVLAVGRLRPPYVDDVQHYKKLLAGHARLELIEVRADDDVS
                     RRIPDRSFKVLLAAGGRSYDSEGFSRFLEERRQSGMNLCFVIGGPRGLDLDEVDMRLS
                     LGEMTLPHQLARVVLLEQLYRGHKILAGEPYHY"
     gene            4385..4675
                     /locus_tag="VHF88_09315"
     CDS             4385..4675
                     /locus_tag="VHF88_09315"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX2162003.1"
                     /translation="MPEPDGERHFDIHADPEVMAGHYANFANVSHSDYEFTITFARVD
                     HEIEEDSVPGVVVSRINLSPRFMRELIDAMEDNFSKWHTREGIKNLPEAPDR"
     gene            4727..5488
                     /locus_tag="VHF88_09320"
     CDS             4727..5488
                     /locus_tag="VHF88_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162004.1"
                     /translation="MLTPADIQLELTQEFLGSPGQRAGFLVLAGLLGGFLFIRTSARL
                     IRMQVSWWPGNVETSSGLHIHHLVWGIVTIMASGFLMFSFQPDSPWMEILAVAFGIGC
                     GLTLDEFALWLHLEDVYWTAEGRSSVDAVIVASLIGGMLVLGAAPFDSNEQGSVLAIG
                     LTIAVNMAFVVIVVAKGKLLTAVLGAFVPLMALIGAIRLAKPNSRWAKRRYAPGGRKL
                     RRATERNRWVEGVQDRIFNAIGGRPSDGANSGPGS"
     gene            5507..7045
                     /locus_tag="VHF88_09325"
     CDS             5507..7045
                     /locus_tag="VHF88_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162005.1"
                     /translation="MQSELAPTSPRRRTPLTAVLALTLAVLALLPAAAAASPTQLSII
                     QDDATFVYGSGRDPDAAVAEAKMLGADAIRVFVSWHSVAPNQDSRERPPGFDARDPDS
                     PGYRWGMYDALVERARRNGLKLFVALSPSIPYWASEQPSRCPHRIGGYSHLTMSCMWK
                     PSPALFAQFVEAAVKRYGTRAEGPFGGQVALWSLWNEPNLEHYIWPQLKRTRHGIVDL
                     AAVRYRELWLAGWKAIARHDPPSRNKVLFGETAAISSPMDTLYAALCLDEQGDPFKGR
                     LRALHGCKKVKRLPIAGLALHPYNNFAIGSVFTRSFTKDSLPMAYMSRAHKLLDRAAR
                     FRRIPPRRGIYVTEFGFQSSPPNPFGDALSLTRHAAAINEADRLFYGDRRVRSIAQYE
                     LFDVGNPGEFNTGLRFADGERKPAFEAYRMPLVVTRLDANRVEVWGQVRPASGRTQAV
                     IEIAGTGSVVRRQRTNASGYYRFVLRRAGAARLRYRATWAAPDGETLQSRVAAPGRPI
                     KYRE"
     gene            7042..8094
                     /locus_tag="VHF88_09330"
     CDS             7042..8094
                     /locus_tag="VHF88_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162006.1"
                     /translation="MKQRSVWLEAGAVMAVALPLLLLMDAYVRDSPTPRNDELIYELM
                     AQAPFDPHTFPFAHRVAVPTLVHLLPFSHTFSFSLLAWLSTAAVGGLAYVLMRRFAIA
                     KPLAAGLAIGLALSPTLFVASLREGRNVDPESVLIMFAGALAIVDRRPAIFGAIVLVG
                     CFVREAALFLVPFAYAVWAERLWDPRAARQTLLAAGPAVAAYAALRLSVPSLHRERVL
                     GYDSLLGGRLEVLRKAAEQPLTILRRIGLAFGALWLAAPFALRDMRFARRGLVLLAAC
                     CVSMLFALDWGRIVFLAAPVIVVAAAWVLNTRPRLAIAAVAALLAMDAGYAIYMEDFG
                     GADNITDPAPTRYPTV"
     gene            complement(8161..>8403)
                     /locus_tag="VHF88_09335"
     CDS             complement(8161..>8403)
                     /locus_tag="VHF88_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162007.1"
                     /translation="GDASAVATADGDATSRRRAADGVRVRLDRPFGLGNDEPTCAPST
                     CPRPLAVVEDARLPGGVRAGPGPITPFGPIVVRVVP"
BASE COUNT         1124 a         3148 c         2775 g         1356 t
ORIGIN      
        1 ctcgcggtgg cgctcgtgtc gttcggcttc ggagctgcct cgaacgtgct ctcgcggcag
       61 gtcgaggcgc gcgccgacgc gttcgcgctc gagctcaccg acgagcccgg cgccttcgtc
      121 gcgctcgagc gcagcctcgc ccggcgcaat ctcggcgatc cggacccgcc gcgcctcctg
      181 cacggcctgt tcggcaccca cccgaccacg ctcgagcgga tcggctacgg ggtcgcgttc
      241 ggacgctccg atcgatgacc ctccagcgcc gccgcggagc tagactcgtg ctctcacgcg
      301 gacgtagctc agttggtaga gcgcgagctt cccaagctcg aggtcgcggg ttcgaggccc
      361 gtcgtccgct ctgtgtcgcg gcggtgctcc gccgcttacc ctctccctga tgaagcgccg
      421 ctctcccttc gccgtagccg ccgtcctcgg cgtcgtcgcc ctgctcgggc tgttgaccta
      481 cgggctcttc gccagcgagc cggaccgcgg catggacgac gcgctggcgc gcggggagac
      541 ggtcgcggcg cccggcttcg agctcgagcg gctctcgggg ggcggcaggg agtctttgcg
      601 cgactaccgc ggcaaggtcg tcgtgctgaa cgcctgggcg tcatggtgcg agccctgccg
      661 cgaggagtcg ccgctgctgc agcgctggca cgagcggctg tcgcgcgacg ggcgcgggac
      721 ggtgctcggc atcgacgcgc tcgacgtcga cagcgacgcg cgcgccttcg tgcgcgagta
      781 ccggctcacc tacccgatgc tgcgcgatcc cgaggccgag tcgctgctcg actacggggt
      841 catcggctat ccggagacgt ttgtgatcga cgagcgcggc aacatcgtcg cgctgcgccg
      901 cggcccggtc gacgagcagt ggatgcgcga caacgtcgcg ccgctgctgg agcgctcgtg
      961 aagcgggcgc tcagcctggc ggcggcgctg atgctgctcc tgcttccggg cggcgcggtc
     1021 gcggccgact gcccgaagac cagcctcgcc gcggtcgagg ccgacgtgat gtgccccgtc
     1081 tgcggtaccc cgctcggcct ggcgaccgag gcgccgcagg ccaaccgcca gcgcgcgctg
     1141 atccagcgcc tggtcgaccg ctgccggtcg aaggaggaga tcgaggaggt tctcgtcgcg
     1201 cagtacggcc ccggggtgct ggcgctgccc gagacggacg gcttccagct cagcgcgtac
     1261 ctggtgccgg ccctgatcgt cctgctcgcc gccggcggca tcgcgctggc ggcgcggcgg
     1321 tggcgcggcg gtggaggacg accggccacg gccggccccg cagaggcgga tgtcgcgagc
     1381 aagcggctcg acgacgacct ggagcgctac gagctgtgat cggcgccgac gccgtcgaca
     1441 cgacggtcgt cgcggctttc gccgtcggct tcatctcgtt cgtctcgccc tgcgtgctgc
     1501 cgctcgtgcc cggctacctc tcgacgatct ccggcgtctc cttcagcgac atcgaggccg
     1561 gcagatcgcg ccgcgaggtg ctctggccgg cgctgctgtt ctgcctgtcg ttctcgctga
     1621 tgttcgtcgc gctcggcatg acggctaccg gcctcggcca gacgctccag gaccaccgcc
     1681 agctgctgcg cgagatctcg gggatcgtcc tgatgctgct cggagccttc ttcatcgcga
     1741 cgctgttcat cagccgcctc aaccgcgagt ggcgccctga ggcgctgctc tcccacgccg
     1801 gcaccggcgg cccgatcgtc gccgggctcg cgttcgcgat cgcctggctg ccttgcaccg
     1861 gcccgacgct cggcgccgtg ctgaccgccg cctccaacga gtcgacggtc ggccaaggcg
     1921 gcgtcctgct cgccttctac gcgcttgggc tcgccgtccc cttcgttctc agcgcgctcg
     1981 ccttctccca cgtctccggc gtcttccgct tcttccgcaa ccactacgcc gcgatcacgc
     2041 tgatctcagg cgcgatcctg atcgggatgg gctacctgct cttcgccggc gagctcaccc
     2101 gcctcaacgc cgaggcgctg gatttgatgg acgacctcgg aatcaacatc ttctccgatc
     2161 tgtgatcggt cctacatctc gtccggggcc tcgaccccga gcaggtcgag cgagcgggcg
     2221 atgacgcggc gggtgagctc gcagagggcg aggcggaggt cctcgtcgcc gccctcctcg
     2281 gcggctccga tgaccttgca gtcgcggtag aaggccgaga acgactgcgc gacctcggtc
     2341 acgtagacgg ccatgcggtg tggcgcgcgg cggtcggccg cgattcgcac ctcctcgggc
     2401 agctcgagca gcttcttgac gaggtcgcgc gcgctcgggt gcagcacctc gcggctgacg
     2461 gcaagatcgc ccgcgacggc cgcagccacc tgcccctccg ccgcccggcg caggatgctc
     2521 gcgatccgcg catgggcgta ctgcgcgtag tagaccgggt tgtcctgact gcgctgccgc
     2581 gccaggtcca ggtcgaggtc gagcgccgtg tccgggctcc gctggacgag gaaccagcgc
     2641 gtcgcgtcga cgccgatgtc gtcgaccagc tcgtcgagcg tcacgaactg cccaccgcgc
     2701 ttcgacatct gcgcccgctg cccgtcctcg agcaggttca ccagctgcat cgtcacctgc
     2761 tccaaccgct gcggatcccc gcccagcgcc tgccatgccg cccgcatccg accgaggtag
     2821 ccatggtggt ccgcgccgag gacatcgacg acatggtcgt agccccgccc ccgcttgtcc
     2881 tcgtggtagg cgatgtcagc cgcgaagtac gtcttctcgc cgttcgaccg ccgcagcacc
     2941 cggtccttgt cgtcgccgaa ctccgtgctc cgcagccacg tcgccccctc gctctcgtac
     3001 acatggccct gctcgcgcag ccgctcgatc gccgcctcca ctgcgccgcc ctcctgcagc
     3061 tcccgctcgc tcgagaagcg gtccatctcc acccgaaacc gcctcagcgt cgcctcgacc
     3121 ccctccaaca tgatcgccac gccccgcttc gccagctcct ccacgtccag ctccccagcg
     3181 ccctcgatcc ccgcagcgac ctccttgacg tactcgccct gatacccgtc ctccggcggc
     3241 gcctcaccca acgcccgcgc ccggatcgac gccccaaacc gctgcacctg cccgccatag
     3301 tcgttgacgt agtactcccg atccacctcg ctccccgcca gctccaaaac ccgcgccaac
     3361 gaatccccat aagccgcatg ccgcccagcc gccacagtga tcggccccgt cggattcgca
     3421 ctcacgaact ccaggttcac ccgctccggc gcctcgacga cgctccgccc gaactcgtcg
     3481 ccatcgccca gtgccgcggc agcggccgac ttgagccagt catcggccat gaacaggttc
     3541 aggaaccccg gccccgccac ctccacacgc tcgacgccat ccccgagcgc cccctccaac
     3601 gcctccccaa gccgctccgc aatcgcccgc ggcggctgct tcaacgcccc ggccaacatc
     3661 atcgccgcat tcgtcgagta gtcccccaaa tccgcctgcg gcggccgctc caacgtaggc
     3721 gccttcgcag gctccccacc cagcgactcc gcagccgcga tcacagcctc gctcagatcc
     3781 ttgacagggt ccgtcataag gctcggcacc ctagacgttt gcctccgcgg gtggcgacga
     3841 ttttgcgttt gggcgcgcag cttgcgagca cccaaaccaa aatcggagac aggccccgcg
     3901 ggcagccccc gcccagaccc acgccgcgcc cgccccagcc ctaactcagt agtgatacgg
     3961 ctccccagcc aaaatcttgt gcccccgata cagctgctcc aacagcacca cccgcgccaa
     4021 ctgatgcggc agcgtcatct cacccaacga caaccgcata tccacctcgt ccaggtccaa
     4081 cccccgcggc cccccgatca cgaagcacag gttcatcccc gactgccgcc gctcctccaa
     4141 gaaccggctg aacccctcgg agtcgtacga ccgcccgccc gccgccagca gcaccttgaa
     4201 cgaccggtcc gggatccgcc gcgacacgtc gtcgtcggcg cggacctcga tcagctccag
     4261 ccgggcatgc ccggcgagca gcttcttgta gtgctggacg tcgtcgacgt acggcgggcg
     4321 cagccgcccg accgccagca cgaggatcct catcgagcct tcaccgggct gcgatactac
     4381 ggccatgccc gagcccgacg gagagcgcca cttcgacatc cacgccgacc cggaggtgat
     4441 ggctggccat tacgcgaact tcgcgaacgt cagccactcg gactacgagt tcacgatcac
     4501 gttcgcgcgc gtcgaccacg agatcgagga ggattcggtc ccgggcgtgg tggtctcgcg
     4561 gatcaacctc tcgccgcgct tcatgcgcga gctgatcgac gcgatggagg acaacttctc
     4621 caagtggcac acccgggagg gcatcaagaa ccttccggag gcgccggacc ggtagccgtc
     4681 ccaccgagct gaaggcaacg ccccgcctcc gtcgaatggt gccggggtgc tgacgcccgc
     4741 cgacatccag ctcgagctga cgcaggagtt cctcggcagc ccgggccagc gggcggggtt
     4801 cctcgtactg gcggggctgc tcggcggctt cctgttcatc cgcaccagcg cacggctgat
     4861 ccggatgcag gtgagctggt ggccgggcaa cgtcgagacg tcgagtgggc tgcacatcca
     4921 ccacctggtg tgggggatcg tgacgatcat ggcctcgggc ttcctgatgt tctcgtttca
     4981 gcccgacagc ccgtggatgg agatccttgc cgtggcgttc gggatcggtt gcggcctgac
     5041 gctcgacgag ttcgccctct ggctgcatct cgaggacgtg tactggaccg ccgagggtcg
     5101 ctcgtcggtc gatgcggtga tcgtcgcgtc gctgatcggg gggatgttgg ttctcggtgc
     5161 ggcgccgttc gacagcaacg agcaggggtc cgtgctggcg attgggctca cgatcgccgt
     5221 caacatggcc ttcgtcgtga tcgtggtggc gaagggcaag ctgctgacgg cggtgctcgg
     5281 tgcgttcgtc cctctgatgg cgctgatcgg cgcgatccgc ctcgccaagc cgaattcgcg
     5341 ctgggcgaag cgccgctacg cgcccggtgg ccgcaagctg cggcgtgcga ccgagcgcaa
     5401 caggtgggtg gagggggttc aggaccgcat cttcaatgcg atcggcggcc ggccgtctga
     5461 tggcgcgaac agcggccccg gatcgtagtc ttcgcactgg ccctccatgc agtccgagct
     5521 cgcaccgaca tccccccgtc gccgaacgcc gctgacggcg gtcctcgcgc tgacgctcgc
     5581 ggtcctggcg ctgctgcccg cggccgccgc ggcgtcgccg actcagctgt cgatcatcca
     5641 ggacgacgcg acgttcgtat acggcagcgg ccgcgatccc gatgccgcgg tcgcggaggc
     5701 gaagatgctc ggagccgacg cgatccgggt cttcgtcagc tggcactcgg tcgccccgaa
     5761 ccaggactcg cgcgagcgcc caccgggctt cgacgcacgc gatcccgact cgccgggcta
     5821 tcgctggggt atgtacgacg ccctcgtcga gcgggcgcgg cgcaacgggc tgaagctctt
     5881 cgtggcgctg tcgccctcga tcccctactg ggcctctgag cagccgtcgc gctgcccgca
     5941 ccggatcggc ggctactcgc acctgacgat gtcgtgcatg tggaagccgt cgccggcgct
     6001 gttcgcgcag ttcgtcgagg cggcggtgaa gcgctacggg acccgggcgg agggcccctt
     6061 cggcgggcag gtcgcgctct ggtcgctctg gaacgagcca aacctcgagc actacatctg
     6121 gccgcagctg aagcggaccc ggcacgggat cgtcgatctg gccgcggtgc gctaccgcga
     6181 gctgtggctg gcgggctgga aggcgatcgc caggcacgat ccgcccagcc gcaacaaggt
     6241 gctgttcggc gagacggcgg cgatcagctc gccgatggac acgctctatg cggcgctctg
     6301 cctcgacgag cagggcgacc cgttcaaggg ccggctgcgc gcgctgcacg gctgcaagaa
     6361 ggtgaagcgg ctgccgatcg ccggcctggc gctgcacccg tacaacaact tcgcgatcgg
     6421 ctcggtcttc acgcgcagct tcacgaagga ctcgctgccg atggcctaca tgtcgcgcgc
     6481 ccacaagctg ctcgaccgcg ccgcccgctt ccgccgtata ccgccccgcc gcgggatcta
     6541 cgtgaccgag ttcggcttcc agtcgagccc gccgaacccg ttcggcgacg cgctctcgct
     6601 cacccgtcac gcggcggcga tcaacgaggc cgatcggctc ttctacggcg atcggcgcgt
     6661 ccggtcgata gcccagtacg agctgttcga cgtcggcaac ccgggtgagt tcaacaccgg
     6721 actgcgcttc gccgacgggg agcgcaagcc ggcattcgag gcgtatcgga tgccgctggt
     6781 ggtcactcgc ctcgacgcga accgggtcga ggtctggggg caggtgcgtc ctgcgagcgg
     6841 ccgtacgcag gcggtgatcg agatcgccgg caccggttcg gtggtccgcc gccagaggac
     6901 caacgcgagc ggctactacc gcttcgtcct gcgtcgcgcg ggcgccgccc gcctgcgcta
     6961 ccgcgcgact tgggccgctc ccgatggtga gacgctgcag agccgcgtcg ccgcccccgg
     7021 acggccgatc aagtaccgcg agtgaagcag cgctcggtct ggctcgaggc cggggcggtg
     7081 atggcggtgg cgctgccgct gctgctcctg atggacgctt acgtgcgcga ctcgccgacg
     7141 ccgcgcaacg acgagctgat ctacgagctg atggctcagg cgccgttcga cccgcacacg
     7201 ttcccgttcg cccatcgcgt cgcggtgccc acgctggtcc acctgctgcc gttcagtcac
     7261 acgttctcct tcagcctgct ggcgtggctg tcgacagccg cggttggcgg cctcgcctac
     7321 gtcctgatgc gccgcttcgc gatcgccaag ccgcttgctg cggggctggc gatcggcctc
     7381 gcgctgtcgc cgacgctgtt tgtcgcgtcg ctgcgcgagg gccgcaacgt ggatcctgag
     7441 tcggtgctga tcatgttcgc gggcgcgctg gcgatcgtcg accggcgtcc ggcgatcttc
     7501 ggcgcgatcg tcctggtcgg ctgcttcgtg cgcgaggcgg cgctgttcct ggtcccgttc
     7561 gcctatgcag tctgggccga gcggctctgg gacccgcgcg cggcgcgcca gaccttgctc
     7621 gcggcaggcc cggcggtagc ggcctacgct gctctgaggc tgtcggtccc ctcgctgcat
     7681 cgcgagcgcg tccttggcta cgactcgctg ctcggcggcc gcctggaggt gctgcgcaag
     7741 gcggccgagc agccgctgac gatcctccgc cgcatcgggc tcgccttcgg cgcgctctgg
     7801 ctggccgcgc cgttcgccct gcgcgacatg cggttcgcgc gccgcggcct ggtgctgctc
     7861 gcggcctgct gcgtcagcat gctgttcgcg ctcgactggg gccggatcgt cttcctcgcc
     7921 gcgccggtga tcgtcgtcgc cgccgcgtgg gttctgaaca cgcggccgcg gctcgcgatc
     7981 gccgccgtcg ccgcgctgct cgcgatggac gccggctacg cgatctacat ggaggacttc
     8041 ggcggcgccg acaacatcac cgacccggcg ccgacccgct acccgacggt ctgaggccta
     8101 cggcgccacg ctgggcggat tccggcggtg ctttcgccgg gattcgccca gcgtcctcgg
     8161 tcaggggacg actcggacga cgatcggccc gaacggggtg atcggaccgg gtccagcgcg
     8221 cacgcctccc ggcaggcgtg cgtcctcgac gaccgcgagc ggccgtgggc acgtcgaggg
     8281 cgcgcacgtc ggctcgtcgt tgccgagccc gaagggacgg tcgaggcgca cgcggacgcc
     8341 gtcggcggca cgtcgccgcg aagtggcgtc gccgtccgcg gtggcgacgg cggacgcgtc
     8401 gcc
//
LOCUS       DATABV010000203         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1752849, whole genome shotgun sequence.
ACCESSION   DATABV010000203 DATABV010000000
VERSION     DATABV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752849"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VHF88_09340"
     CDS             <1..377
                     /locus_tag="VHF88_09340"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162008.1"
                     /translation="SLNLSDDAADLLVEKGWDPSMGARPLRRAIQRYIEDPLADEVLA
                     QNLDPGSTVEVEKAPDDDERDVKITIAKPARKREAVGVGAKGGDDENPELEGGDSASD
                     SGDLPDEPEVLPDVPDAPPDKQ"
     gene            522..1631
                     /gene="disA"
                     /locus_tag="VHF88_09345"
     CDS             522..1631
                     /gene="disA"
                     /locus_tag="VHF88_09345"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX2162009.1"
                     /translation="MPYAGDDVSELEARQEPRLVRALEMIAPGTAVREGIDSIVQART
                     GALICIGDSDELAFLYSGGLKLEVDYTPALLFQLAKMDGAIILSSSGTKIHWANVQLM
                     PDPTILSLETGTRHRTAERVSKQTDALVIAISQARSVVSLYLDGAKYILEDIPVVLAK
                     ANQALATLDKYRSRLDQVSTRLTALEFEGGVTLHDVLTVLQRSELVTRMAVEIERYIV
                     ELGAEGRLIEMQLEETMVGVASEKSALLHDYIAEPSDESFASVLDQLARMLHQDLLDF
                     GMLAELLGYDRKLNTLDHPVSPRGHRVLGRIPRLPKLVVQGIVSEFGGLDEILAAGDG
                     DLEAVEGVGDVRAKDIREGLRRLQEINLVDRYLQT"
     gene            1713..>2035
                     /locus_tag="VHF88_09350"
     CDS             1713..>2035
                     /locus_tag="VHF88_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX2162010.1"
                     /translation="MDLEITREMPHIEYEIGDNVVYPHHGAGKVLKKEQKTVLGEQRE
                     YLTIKILHNDMTVMVPCENAGKAGLRRVIGEEAVAKVLSVLRDDVSEMPKNWNRRFKH
                     NREKI"
BASE COUNT          370 a          670 c          694 g          301 t
ORIGIN      
        1 tgtcgctcaa cctctccgac gacgcggccg acctgctggt cgagaagggc tgggacccgt
       61 cgatgggtgc ccggccgctg cgccgcgcga tccagcgcta catcgaggac ccgctcgccg
      121 acgaggtgct ggcccagaac ctcgatccgg gatcgacggt cgaggtcgag aaggcacccg
      181 acgacgacga gcgcgacgtc aagatcacga tcgccaagcc ggcgcgcaag cgcgaggcgg
      241 tcggtgtggg cgccaagggc ggcgacgacg agaaccccga gctcgagggc ggcgactccg
      301 cctccgatag cggtgacctg ccagacgagc ccgaggtcct ccccgacgtc ccggacgccc
      361 cgccagacaa gcaatagcct gcgtctggcg gcgtcatcgg cctctcgcgt gctggcgcac
      421 gccacgaggc ctgcttcctt gccagacttg gctcttgctc gtctggtccg ccattccgca
      481 ctggaaagcc ttttctgccg cattcccgta caatcgttca gatgccgtac gccggggatg
      541 acgtttcaga gctagaggct aggcaggaac ctcggctagt tcgcgcactg gagatgatcg
      601 cgcccggaac ggcggtgcgc gagggcatcg acagcatcgt ccaggcgcgt acgggcgcgc
      661 tgatctgcat cggcgacagc gacgagctcg cattcctcta ctcgggcggc ctcaagctcg
      721 aggtcgacta caccccggcg ctgctgttcc agctcgccaa gatggacggc gcgatcatcc
      781 tgtcgtcgtc ggggacgaag atccactggg cgaacgtcca gctgatgccg gacccgacga
      841 tcctgtcgct ggagaccggc acgcgccacc gcaccgccga gcgcgtctcg aagcagaccg
      901 atgcgctggt gatcgcgatc tcccaggcgc gcagcgtcgt ctcgctctac ctcgacggcg
      961 cgaagtacat cctcgaggac atcccggtcg tgctcgccaa ggccaaccag gcgctcgcga
     1021 cgctcgacaa gtaccgcagc cggctcgacc aggtctcgac gcggctgacc gcgctcgagt
     1081 tcgagggcgg cgtgacgctg cacgacgtgc tcaccgtgct ccagcgctct gagctggtga
     1141 cgcggatggc ggtcgagatc gagcgctaca tcgtcgagct gggcgccgag ggacggctga
     1201 tcgagatgca gctcgaggag acgatggtcg gcgtcgcgtc ggagaagtcg gcgctgctgc
     1261 acgactacat cgccgagccg agcgacgaga gcttcgccag cgtgctcgac cagctggcgc
     1321 ggatgctgca tcaggacctg ctcgacttcg gcatgctggc cgagctgctc ggctacgacc
     1381 gcaagctgaa cacgctcgac catccggtgt cgccgcgcgg ccaccgcgtg ctcggccgga
     1441 taccgcggct gccgaagctc gtcgtccagg ggatcgtcag cgagttcggc ggcctggacg
     1501 agatcctggc cgccggcgac ggcgacctgg aggcggtcga gggggtgggc gacgtgaggg
     1561 cgaaggacat ccgcgagggc ctgcgtcgcc tacaggagat caacctcgtg gaccgctacc
     1621 tgcaaacgtg accaaggagc cctggcggct ttgcgccggg ctcgtattcg actaggaggc
     1681 ccgtcatttc cgagacgatt gagcaagaga tcgtggacct cgagatcacc cgcgagatgc
     1741 cccatatcga gtacgagatc ggggacaacg tggtgtaccc gcatcacggg gcaggcaagg
     1801 tcctcaagaa ggagcagaag acggtgctcg gcgagcagcg cgagtacctg acgatcaaga
     1861 tccttcacaa cgacatgacg gtgatggtcc cgtgcgagaa cgcgggcaag gccggcctgc
     1921 gccgcgtgat cggcgaggag gcggtcgcca aggtgctctc ggtgctgcgc gacgacgtca
     1981 gcgagatgcc gaagaactgg aaccgccgct tcaagcacaa ccgcgagaag atcaa
//
LOCUS       DATABV010000204         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1754046, whole genome shotgun sequence.
ACCESSION   DATABV010000204 DATABV010000000
VERSION     DATABV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754046"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..855
                     /locus_tag="VHF88_09355"
     CDS             136..855
                     /locus_tag="VHF88_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162011.1"
                     /translation="MTTLIRGELIKAITTRTIFAYAAMAVVLAVGQVLASLLPGWGDL
                     MSQDDKKEALGGLPLLVLLLAIVGAAGEYRHRTAAPAALVAGRDAGRLLVARAGAYAV
                     AGAAVAALATVITLAVGLPLLADRPGPVVSAGDVALIAGGSLVAAALLAVFGVALGAL
                     VRNQVAAVTGTLIVIFVVLPLVQALSGTILDVTPFGAAQGVAGAGLTTLSTGAAAVVL
                     VSWTLVALVAAVVGERRRDIA"
     gene            852..2015
                     /locus_tag="VHF88_09360"
     CDS             852..2015
                     /locus_tag="VHF88_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2162012.1"
                     /translation="MTAAREPSQDARRPLGRRAWAFDAAVALAAAAVELGWVLGYDRS
                     PPPVAVGLAFLAGAVLVWRRRQPLVILGVSLAAVLGVTIAGASPSGFAPLVALYTVAA
                     MCERRSSLAGLALTAPITLVASVAGPADEQLVDPIAAVLCSGAAWGLGVYVETRREFR
                     RELDRRAALLERERDQLARIGVHEERASIARELHDIVAHSVSVMLVGVRGARDVLRSD
                     PDIADATLGKVETSAEQSLAELRRILALLREPSDGAESRPQPSLAQLDELVAEYRDAG
                     LPVALERIGTQRSLPGGFELSVYRIVQEALTNVLKHAHPKRVVVTLAFRDSRLDLEVI
                     DDGSPQRQHAVVPGHGLVGMRERVALLGGELEAGRRAGGGFRVAARLPVGGDA"
     gene            2012..2683
                     /locus_tag="VHF88_09365"
     CDS             2012..2683
                     /locus_tag="VHF88_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2162013.1"
                     /translation="MSIRVLIVDDQELVRTGFRLFLDTQPGLEVVGEAADGREAIDRV
                     RQLRPDVVLMDIRMPNMDGVEATAKLTSGAIEPAPRVLVLTTFDLDEYVFGALRAGAA
                     GFLLKDASRERLVEAIRVVHDGEALLSPSITRRLIEDFAARTDPIAPPEAVLAELTPR
                     EREVLVLVAHGLSNTEIAARLVVTEATVKSHVGSILLKLGLRDRVRAVVFAYEHGIVV
                     AGETD"
BASE COUNT          372 a         1058 c         1120 g          446 t
ORIGIN      
        1 gttgatcgag gggatggacg cgacgcaggt gggcgagctc gcccaccgcg agcgcgtgct
       61 cctgcaagag ctcgcgtccg acgacgcctc gctcgagcag gtgttcttta gcctgaccga
      121 ggaagtggag gcggcatgac cacgctgatc agaggtgagc tgatcaaggc catcacgaca
      181 cgcacgatct tcgcctacgc ggcgatggcc gtcgtcctgg cggtcgggca ggtcctcgcg
      241 tcgctcctgc ccggctgggg agacctgatg tcgcaggacg acaagaagga ggcgctcggc
      301 ggcctgccgt tgctcgtgct gctgctcgcg atcgtcggcg ccgccggcga gtaccgccac
      361 cggactgccg caccggcggc gctcgtcgcg ggccgcgacg ccgggcggct gctcgtcgcg
      421 cgcgcgggcg cctacgccgt ggcgggcgcc gcggtcgccg cgctggccac ggtgatcacc
      481 ctggcggtcg ggctgccgct gctggccgac cggcccggcc cggtggtgag cgcgggcgac
      541 gtcgcgctga ttgccggcgg gagcctcgtc gcggcggcgc tgttggcggt cttcggcgtc
      601 gcactcggcg cgctcgtgcg caaccaggtc gccgccgtca ccgggacgct gatcgtgatc
      661 ttcgtcgtgc tgccgctcgt gcaggcgctc agcggcacga tcctcgacgt cacgccgttc
      721 ggcgccgcgc agggcgtcgc cggagccgga ctgacgacgc tgtcgacggg cgcggcggcc
      781 gtggtgctcg tcagctggac gctggtggcg ctggtcgcgg cggtcgtcgg ggagaggcgg
      841 cgcgacatcg catgaccgcg gcccgcgagc cgtcgcagga cgcgcgtcgg ccgctcggcc
      901 ggcgcgcgtg ggcgttcgac gctgcagtgg cgctcgccgc ggcggcggtc gagctgggat
      961 gggtgttggg ctacgaccgt tccccgcccc cggtggcggt cgggctggcg ttcctggccg
     1021 gggcggtcct cgtctggcgt cgccggcagc cgctcgtgat cctcggtgta tcgctcgccg
     1081 ccgtgttggg cgtgacgatc gcgggcgcgt ccccgtcggg gttcgcgccg ctggtcgcgc
     1141 tctacacggt ggccgccatg tgcgagcggc gcagctcgct cgccggcctc gcgctcacgg
     1201 ccccgatcac gctcgtcgcg agcgtcgccg gtcccgcgga cgagcaactc gtcgacccga
     1261 tcgccgcggt gctgtgctcg ggcgcggcgt gggggctggg cgtctatgtc gagacgcggc
     1321 gggagtttcg gcgcgagctg gatcgccggg cggcgctgct cgagcgcgag cgcgatcagc
     1381 tggcccggat tggcgtgcac gaggagcgcg cgtctatcgc ccgcgagctg cacgacatcg
     1441 tcgcgcactc ggtcagcgtg atgctggtcg gcgtgcgcgg cgcgcgcgat gtactgcgca
     1501 gcgatcccga catcgcggac gcgacgctcg ggaaggtcga gacgagcgcc gagcagagcc
     1561 tcgccgagct gagacggatc ctcgcgctgc tgcgcgagcc gtcggacggc gcggagtcgc
     1621 gcccgcagcc gtcgctcgcg cagctcgacg agctcgtcgc cgagtatcgc gacgcgggtc
     1681 tcccggtggc gctggaacgg atcggcacgc agcgatcgct gccgggcggc ttcgagctct
     1741 cggtctaccg catcgtccaa gaggcgctga cgaacgttct caagcacgcg caccccaagc
     1801 gggtcgtcgt gacgctggcc ttccgcgatt cgcgcctcga cctcgaggtc atcgacgacg
     1861 gctcgccgca acgccagcat gccgtcgtgc ccggccacgg gctggtcggc atgcgcgagc
     1921 gggtcgcgct gctcggcggc gagctcgaag caggccgccg cgcgggcggc ggctttcgcg
     1981 tcgcggcgcg gcttccggtc ggaggtgacg cgtgagcatc cgcgtgctga tcgtcgacga
     2041 ccaagagctc gtgcgcaccg gcttccgact gttcctcgac acgcagcccg ggctcgaggt
     2101 cgtcggcgag gctgccgacg gccgggaggc gatcgaccgc gtgcgccagc tgcgacccga
     2161 cgtcgtcctg atggacatcc gcatgcccaa catggatgga gtcgaggcga cagcgaagct
     2221 gacgagcggc gcaatcgagc cggcgccccg agtgctcgtg ctcacgacat tcgacctcga
     2281 cgaatacgtc ttcggcgcgc tgcgagcggg cgccgctggc ttcctgctca aggacgcgtc
     2341 gcgcgagcgg ctggtggagg cgatccgcgt cgtgcacgac ggcgaggcgc tgctgtcacc
     2401 gtcgatcacg cgccggctga ttgaggactt cgccgcccgc accgacccga tcgcgccacc
     2461 cgaggcagtg ctcgccgagc tcacgccgcg cgagcgcgag gtgctcgtgc tcgtcgccca
     2521 cggcctctca aacaccgaga tcgcggcccg gctcgtcgtc accgaggcga cggtcaagag
     2581 ccacgtgggt tccatcctcc tcaagctcgg actgcgcgac cgcgtccgag ccgtcgtgtt
     2641 cgcgtacgag cacggcatcg tggtcgcggg cgagaccgac tagacacgcg tcgggcgctg
     2701 ccgcacgcct cggcgagcac ctcccccctc tcggcgatct caagcgcgaa cgcagcccgg
     2761 cgatgtcgca cccatgccgc gagcgcatgt cgtgcttgtc actctcgcgc gtggtggcga
     2821 gagcacaccg cgcgctctcc cgcgcgctcg cttttcgcct tgatgcggtc gtcttaacat
     2881 ggcgctgccg acgctcgtaa cggacgaccg gcagaagcgc tacgcgagcc gccggatcgc
     2941 acgtggcgca taggcaagac ctaggcaggg catgaggcgc cgaacggatg gaggcc
//
LOCUS       DATABV010000205        15619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2076965, whole genome shotgun sequence.
ACCESSION   DATABV010000205 DATABV010000000
VERSION     DATABV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15619
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076965"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VHF88_09370"
     CDS             complement(<1..277)
                     /locus_tag="VHF88_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162014.1"
                     /translation="MGLVVDPSASLGLMQVEMAINSAVLAWNERLSVGMTTMQTTMAN
                     GLTQLGAGSSIGLMQVESAINSSVLAWNERLQVGLTTMQTTMANGLTQ"
     gene            296..1282
                     /locus_tag="VHF88_09375"
     CDS             296..1282
                     /locus_tag="VHF88_09375"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX2162015.1"
                     /translation="MQGAVREIALEMVRADGTRMPVLVNSTLHKDSAGVPVEIRTAIF
                     GAADRREYERELLRSRDREQDARERAEVLQRTMGRLAAENAELYEHERRVARALQASL
                     LAGEPPRDARFAAAAHYSPAGDTLEVGGDWHDAIRLDPDRVAIVVGDVVGRGIDAAAT
                     MGQLRSAILALASTGMAPAGLLARLDTFVERVASAWMGTVLYAEVDLSDGGVRYASAG
                     HPPPVLVQPGEHPRALWDGRSTPLGAGSGGVARDEAVLALRPGGRLLLFTDGLVERRD
                     RSVDEGLERLLDEVARRSSAPLTQLVEELPLAMDAGGGEDDVCLLAFELLGA"
     gene            complement(1298..1801)
                     /locus_tag="VHF88_09380"
     CDS             complement(1298..1801)
                     /locus_tag="VHF88_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX2162016.1"
                     /translation="MEVIDELVANNRRFAESLPVQHLDVRPSRRLAIVTCMDSRLDVF
                     AALGLGQGEAHVLRNAGGVISDDVIRSLAISQRKLGTREVMLIHHTNCGMQTLSDDSF
                     RAELQEATGVAPAFAIESFNDVGADVRQSILRVRRSGFLLHRDRVRGFVYDVDSHRLD
                     EVHVDES"
     gene            complement(1850..2287)
                     /locus_tag="VHF88_09385"
     CDS             complement(1850..2287)
                     /locus_tag="VHF88_09385"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX2162017.1"
                     /translation="MTDRGRTPAQVYSLVFGAVLLLAGILGFFADSSFGNLGSDVQGD
                     ELIIFEVNGWHNLVHIASGLLGLALMGRADTARAYALGFGAVYLLVTIWGIVDGNDVL
                     GVLPVNAADNVLHIAIAATGILAGLLSSPDRTTAGRPRGATSA"
     gene            2387..2788
                     /locus_tag="VHF88_09390"
     CDS             2387..2788
                     /locus_tag="VHF88_09390"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX2162018.1"
                     /translation="MPKTTSKGTAKQSELPSTLRRSSAKAQRTWAKAHDSAAEEYGDG
                     ERAHRTAYAALKHSFEKKGDRWVAKDSKGPSDPRAKNPRAREGKGKTYGGVDAEGNSK
                     QELYDRARKLGVEGRSKMSKGELAEAIAKKQ"
     gene            2891..3244
                     /locus_tag="VHF88_09395"
     CDS             2891..3244
                     /locus_tag="VHF88_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX2162019.1"
                     /translation="MKLVIGIVRPEKANDVLEALYRAEVRGVTLSRVQGHGGELDRVE
                     TYRGTTVKMGLAEKLRFEIAVSDDFVDPTVEALCAGARTGDVGDGKIFVVPLERAVRI
                     RTGETDHGAVTPVGT"
     gene            3259..4560
                     /locus_tag="VHF88_09400"
     CDS             3259..4560
                     /locus_tag="VHF88_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX2162020.1"
                     /translation="MTLIAQAAQVDTGDTAWMLVATALVVLMTIGLGLFYAGLVRSKN
                     TLNTFMMCIAALAVATVTWALVGYSIAFDGDGDIVGGLGHAFMQGVDFAPRDGTTIPH
                     LVFFAFQASFCIITVALVSGAVVERMRFGAFLVFATLWSVLVYAVLAHWAFGGGWLME
                     GGTLDFAGGVAVEMGSGFSALAAALVVGARKDYGRQALLPHNAVYVVLGAGLLWFGWF
                     GFNGGSGFGTGQASILAFTNTLLAPAATLVVWFVLDLIRGRKVTAIGAATAIIVGCVV
                     ITPAGGFVSPGWALALGAIAALPCYAMIVWRPRTRVDETLDVLAAHGVAGFSGILLLG
                     IVAQASWNGVSDGLLYGNADQFLWQAIAAAATPAYAFIATFVLLKLIGAVMPLRASHH
                     DEAIGMDIVQHGEEAYVTGEGAILISSPDGEEPGVPVANPI"
     gene            complement(4557..5999)
                     /locus_tag="VHF88_09405"
     CDS             complement(4557..5999)
                     /locus_tag="VHF88_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2162021.1"
                     /translation="MRARRALAAICVALATAGAAVGASAVFELDRELSTGTVRISVEP
                     GHEGALDLYMPLVDWGVRFPGAVRLPARLSVGLRTVDRAALERVAGGDVPELEQVRDE
                     GRDAIAAYLRLLLAVVAVAALAAGVLVALALRGRRTPLAPLVATAAATALVCCAAIAL
                     LLPPRGDLGDPDYYAHGPDIPAALRAVEQATRSSAVLSEELDAQLVGLARLVSEPSDR
                     VREGDARQLTLASDLHSNLLALPTLERAVGEGPLFFAGDLTSNGSPFEVALTRRVARI
                     GSRLVFVTGNHDSDLLARRLAAAGAVVLTERGRLRADGSLGPVVVRVAGLRVAGYGDP
                     DQRRRADGYRDRYDDDGDDIDRQRRFADWLLPLAGKIDVAMVHQPKLARLAVAELRAD
                     PPRGPLVLLSGHTHQPALRISDNLVELNGGTAGGGGAGNLDEHQPLGLAVLTYATGDR
                     FEPLAADTVEIDPGSGAASAERTPIPAGVR"
     gene            complement(6069..6818)
                     /locus_tag="VHF88_09410"
     CDS             complement(6069..6818)
                     /locus_tag="VHF88_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEX2162022.1"
                     /translation="MSRRTLTLALIAVLALALTAPALGNGDSMLTRALGIAKSAERKA
                     NAAQRDARTAKRIARQSKRIAREKARRGPHGPRGAIGPTGSPGSTGARGPSGPAGAPG
                     RDASVAFDQETPDVSTSSTTYVSLGGPSVEVTVPESGLIEVAAQATLRDGSDDDGAVA
                     LFEDGSPVAGQADCGPPGAMLFSLRSGSGDDFSGGTPAGSLLCATLGPPAPVRLLATP
                     GTHTYELRYAVCCGTGSATFADRRLWVSPLP"
     gene            6908..8563
                     /gene="ettA"
                     /locus_tag="VHF88_09415"
     CDS             6908..8563
                     /gene="ettA"
                     /locus_tag="VHF88_09415"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX2162023.1"
                     /translation="MAEFIYTMRELTKVHPPDKEVLKDVTLAYLRGAKIGVLGPNGAG
                     KSTLLRIMAGVDTEFRGDAQLAEGATVGLLEQEPALDPAKDVRGNVEDGVRPLRDLLD
                     RFNELAANYSEDTADEFARLQKEIDAADAWNLDTLLDTAMDALRLPPGDADVTKLSGG
                     ERRRVALCRLLLGRPDLLLLDEPTNHLDAESVAWLERHLADYPGAIVAVTHDRYFLDN
                     VAGWILELDRGRALPFEGNYSSWLEQKQKRLAQEEKQESARQRTLASELEWVRMNASS
                     RLTKNKARLARYDALLAEERNVKLDNVEIHIPAGQRLGDVVVEAEGLSKGYGDRLLID
                     DLSFKLPPAGIVGVIGGNGAGKTTLLRMITGEEQPDSGTLRVGETVELSYVDQSRDAL
                     DPDKTVWQEISGGDDQLKVGDRTLNSRAYVSSFNFKGSDQQKKVGSLSGGERNRLHMA
                     KLLRTAGNLLLLDEPSNDLDVDTLRALEEALLAFPGCAVVVSHDRWFLDRVATHVLAF
                     EGDSKVTWFEGNFDAYETWRREQLGEAADHPHRMTYKKLIARR"
     gene            complement(8560..8991)
                     /gene="dtd"
                     /locus_tag="VHF88_09420"
     CDS             complement(8560..8991)
                     /gene="dtd"
                     /locus_tag="VHF88_09420"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX2162024.1"
                     /translation="MRAVVQRVGEASVTVEGELVARIGAGLLVLLGVHRDDGEAEADR
                     MAEKLLALRIFPDDDGRMNRSVADTAGELLCVSQFTLYGDARKGTRPSFGEAAPGEVA
                     EPLYERVRARLGAQGGVFGARMEVSLVNDGPITLVVEVGPG"
     gene            9226..9864
                     /locus_tag="VHF88_09425"
     CDS             9226..9864
                     /locus_tag="VHF88_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162025.1"
                     /translation="MPTPPTGRSVKIDQDERGGWSSDRPRRDGAPPRPADLSVRCRVL
                     RPVELLRYSPGSVVIVVSAVIADGERFAERLIEERGVLMSPGKVRGLLAGRVPEEEIE
                     PRAAELLHAAALKRIEAKESVVVVTGDIGAEERERYVRPAAAQKRPCHIILVEPARDD
                     VDEEDRRTLNKLRTALDAGDLGAEGFNTALRLGPASAGEVKRIVFRAPPRDD"
     gene            complement(10088..12169)
                     /locus_tag="VHF88_09430"
     CDS             complement(10088..12169)
                     /locus_tag="VHF88_09430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX2162026.1"
                     /translation="MTSSAPAQPFADAALERLRALTGDPAAEFRDDQLEAIRDVVEDR
                     ARVLCVQRTGWGKSAVYFVATALLREAGAGPTLIVSPLLALMRNQIAAAERLGLRAHT
                     VNSTNQDEWDDVRRMLGEGTVDLLLISPERLNNPRFRDDMLPLFAASVGLLVIDEAHC
                     ISDWGHDFRPDYRRVKDMLDALAPDVAVLGTTATANDRVVADVEEQLSDGADLRSYRG
                     PLARTSLRLEALELPRPAERLAWLVENLPQLPGSGIVYTLTKRDAEQVASFLSAQGVP
                     ALAYSGEQDSELRIATEERLLRNDVKAVVATSALGMGYDKSDLTFVVHYQAPGSVVSY
                     YQQVGRAGRGVDHADVVLLRGGEDRKIQDFFIAQAFPPYERVRAVLDELAAAGGAGRT
                     TRELMAVVNLGMGRIDAMLKILDVEGAVRRDGRRWYPNPGSDWTYDVTRYEQVTALRR
                     AEQEAMAAYGVDGRCLMRVLQEELDDPDPRDCGRCSVCTASRFATPPDPALVELAQRH
                     LRSRPIELEAKKMAPDPETGAMRKIAEDARTDSGWALARVGDGGWWPAIERGLAAGRL
                     GDEIAAALADVVRNGGAKPAWVTAIPSARLPDGLLDDLATRVAAELGVPRVAVLERTG
                     ERPPQREMANAVQQAANVRGAFRVTATPPPGTGLLIDDQRLSGWTMAMVGGQLRRQGA
                     ARVIPLALATL"
     gene            12304..12486
                     /locus_tag="VHF88_09435"
     CDS             12304..12486
                     /locus_tag="VHF88_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162027.1"
                     /translation="MTQHLDCRLDQFGRRFDEVDRRLDRIDERIRLLTTWVTWVGGST
                     IGLLIGLIAAAVIDHG"
     gene            12593..12665
                     /locus_tag="VHF88_09440"
     tRNA            12593..12665
                     /locus_tag="VHF88_09440"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12626..12628,aa:Gly,seq:gcc)
     gene            complement(12847..13467)
                     /locus_tag="VHF88_09445"
     CDS             complement(12847..13467)
                     /locus_tag="VHF88_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162028.1"
                     /translation="MAVVDASRLRDAAVLTIACHEIVECALGAAKGTADEAHSDGLDD
                     VMKRVMWEEYVVERRRRTIFEAEGWGDSIIDHSHLNAVTDDYERDLPRLVRWAAVNDA
                     VPYEVYGHWQLVTIEVAHASARADAGFSPERSEIDTFLARPWSPATAAAWRSARDLLP
                     RLYKQPDAATDDLDAQVISGAWRPISDALAEDYNVLYDRAARDQPV"
     gene            complement(13770..14153)
                     /locus_tag="VHF88_09450"
     CDS             complement(13770..14153)
                     /locus_tag="VHF88_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2162029.1"
                     /translation="MASFLHTCYRIGDIDRSVEFYETLGFEERRRMPIGDEAINVFMG
                     LPGEDPRLELTYNHGVDSYDLGTGYNHIAIGVDDLDGTLAGLAQSGIEPEKPPYRPGG
                     RTTGSRIAFVRDPDGYRIEIVERQP"
     gene            complement(14164..15456)
                     /locus_tag="VHF88_09455"
     CDS             complement(14164..15456)
                     /locus_tag="VHF88_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HEX2162030.1"
                     /translation="MEDSAWTARYGSVTGDDICTFIYTSGTTGPPKGCVISHANYRAM
                     VDMVVSEDVVASGDVVYLFLPLAHSFALLIQLGVFELGSTMAYWSLDPKKIIDDVAEV
                     RPTYLPSVPRIFEKIYTKATAAEGAKKKILDWSIATGRKYREAERAGRSPGFLLEKQF
                     ALADKLALSKIRALFGGRVRLCVTGAAPINREILEFFWAAGVLILEGYGMTETSTAAT
                     INRPDRYKFGTVGLPFPGCEVKIADDGEICVKGPNVFQGYHKKDDATRETLIDSWLHT
                     GDLGEIDSDGFVRITGRKKDIIITAGGKNITPANFEGDMKQHPLVSQCVMVGDRLPYL
                     TALVTLDPEEGPEYAKAHDLPTDVAALAASPEIRAELERHMAEVNKKYAQVEQVKKLT
                     ILPHDLSQETGELTPTLKVKRSVVVDKYADEIDRMYAG"
BASE COUNT         2318 a         5484 c         5454 g         2363 t
ORIGIN      
        1 gctgagtcag cccgttggcc atggtcgtct gcatggtcgt cagaccaacc tgcagccgct
       61 cgttccaggc gagcaccgac gagttgatcg cggactcgac ctgcatcagg ccgatcgacg
      121 agcccgcgcc cagctgcgtg agcccgttgg ccatcgtggt ctgcatggtc gtcatgccga
      181 ccgagaggcg ctcgttccag gccagcacgg cggagttgat cgccatctcg acctgcatca
      241 ggccgagcga ggcggacgga tctaccacga gacccactac gcaccgctct tggcgatgca
      301 gggggccgtt cgcgagatcg cgctcgagat ggtgcgggcg gacggcactc gcatgccggt
      361 gctggtcaac tcgacgcttc acaaggactc cgccggggtg ccggtcgaga tcaggacggc
      421 gatcttcggc gccgccgacc gccgcgagta cgagcgcgag ctgctgcgct cgcgcgaccg
      481 cgagcaggat gcgcgcgagc gggccgaggt cctacagcgg acgatggggc gcctggccgc
      541 cgagaacgcc gagctctacg agcacgagcg gcgcgtcgcg cgtgcgctgc aggcgagcct
      601 gctcgccggc gagccgccgc gcgacgcgcg cttcgccgca gcggctcact acagcccggc
      661 gggggacacg ctggaggtgg gcggtgactg gcacgacgcg atccggctcg accccgaccg
      721 cgtggcgatc gtcgtcggcg acgtcgtcgg gcgcggcatc gacgcggccg ccacgatggg
      781 ccagctgcgc agcgcgatcc tcgcgctcgc cagcaccggg atggcgccgg ccggtctgct
      841 cgctcgtctc gacaccttcg tcgagcgggt ggcgtcggcc tggatgggaa ccgtcctcta
      901 cgccgaggtc gacctgtcgg acggcggcgt ccgctacgcc tccgccggcc acccgccacc
      961 cgtgctggtc cagcccgggg agcatccgcg agcgctttgg gatgggcgct cgacgccgct
     1021 gggcgccggc tccggcggcg tcgcgcgcga cgaggccgtc ctggctcttc gccccggcgg
     1081 acggctgctg ctgttcaccg acggcctcgt cgagcgccgc gatcggtccg tggacgaggg
     1141 ccttgagcga ctgctggacg aggtggcgag gcgtagctcg gctccgctga cccagctcgt
     1201 ggaggagctg ccgttggcga tggacgccgg cggcggcgag gacgacgtct gcctgctggc
     1261 cttcgagctc ttaggtgcct gagcggtagc gccgtcgtca gctctcgtct acgtggacct
     1321 cgtccagccg gtggctgtcg acgtcgtaga cgaagccgcg cacgcggtcg cggtgcaaca
     1381 ggaagccgga gcggcggacg cgcaggatcg actggcggac gtccgcgccg acgtcgttga
     1441 acgactcgat cgcgaacgcc ggcgcgactc cggtcgcctc ctggagttcg gcgcggaagc
     1501 tgtcgtcgct gagcgtctgc atgccgcagt tggtgtgatg gatcaacatc acctcgcgcg
     1561 tgccgagctt gcgctgcgag atcgcgagcg agcggatcac gtcgtcgctg atcaccccgc
     1621 cggcgttgcg cagcacgtgc gcctcgccct ggccgagccc gagcgctgcg aagacgtcga
     1681 gtcgcgagtc catgcacgta acgatcgcca gccggcggct cgggcgaacg tcgaggtgct
     1741 ggaccgggag agattccgcg aagcggcggt tgttggcgac cagctcgtcg atgacttcca
     1801 cgcgcggcag cctagacgga agcagccgcg cgcctcaccc tggaaggact caggccgacg
     1861 tcgcgccgcg cggccgcccg gccgtcgtcc ggtccggcga cgacagcagc cccgccagga
     1921 tgccggtggc ggcgatcgct atgtggagca cgttgtcggc ggcgttgaca ggcagcacgc
     1981 cgagcacgtc gttgccgtcg acgatccccc agatcgtcac gagcaggtag acggccccga
     2041 agccgagcgc gtacgcgcgc gcggtgtcgg ccctgcccat cagcgccagg ccgagcagtc
     2101 cggaggcgat gtgcacgagg ttgtgccagc cgttgacctc gaagatgatc agctcgtcgc
     2161 cctggacgtc gctgccgagg ttgccgaagc tggagtcggc gaagaagccg aggatcccgg
     2221 cgagcagcag cacggcgccg aagacgaggg agtagacctg agctggtgtg cgtccgcgat
     2281 ctgtcatgtc gtcgcgctac ccgttgcgtc gaccggtaaa ccgtttcctg ccactgcggc
     2341 tcgggtaggg gttttcggag acggagctgc aagaggaggc gattcgatgc cgaagacgac
     2401 cagcaagggg accgccaagc agagcgagct tccgtcgacc ctgcggcgct cctcagcgaa
     2461 ggcccagcgg acctgggcga aggcccacga cagcgccgcc gaggagtacg gcgacggcga
     2521 gcgtgctcac cgcacggcct acgccgcgct caagcactca ttcgagaaga agggcgaccg
     2581 ctgggtcgcc aaggactcca agggcccgtc ggacccgcgt gccaagaacc cccgcgctcg
     2641 cgagggcaag ggcaagacct acggtggggt cgacgccgag ggcaactcga agcaggagct
     2701 ctacgaccgc gccaggaagc tgggcgtgga gggccgctcg aagatgtcca agggcgagct
     2761 cgcggaggcg atcgccaaga agcagtaggc cgccgtcctt gaccgcccgc gcgcgatccg
     2821 gttaggctct cgcgcgctcg cgggaggcgt gcatcgctcc cgacctggcc tgtccaagaa
     2881 ggagcggcaa gtgaagctgg tgatcggcat cgtccggccc gagaaggcca acgacgtgct
     2941 ggaggcgctc taccgcgccg aggtgcgcgg tgtgacactg agtcgcgtac agggacacgg
     3001 cggcgagctc gaccgggtcg agacctaccg cgggaccacc gtgaagatgg ggctcgcgga
     3061 gaagctgcgc tttgagatcg cggtctccga cgacttcgtc gacccgacgg tcgaggcgct
     3121 ctgcgccggc gcgcgtaccg gtgacgtcgg cgacgggaag atcttcgtcg ttccgctcga
     3181 gcgggcggtt cgcatccgca ccggcgagac cgaccacggc gccgtgacgc cggtgggcac
     3241 gtgacgcgcc tcggcgacgt gacactcatc gcgcaggccg cacaggtcga caccggcgac
     3301 accgcgtgga tgctggtcgc gacggcgttg gtggtcttga tgacgatcgg cctcgggctc
     3361 ttctacgccg ggctcgtccg ctcgaagaac accctcaaca cattcatgat gtgcatcgcc
     3421 gcgctcgccg tcgcgactgt cacctgggcg ctcgtcggct actcgatcgc cttcgacggc
     3481 gacggcgaca tcgtcggcgg cctcggccac gccttcatgc agggcgtcga cttcgctccg
     3541 cgcgacggta cgacgatccc gcacctcgtc ttcttcgcct tccaggccag cttctgcatc
     3601 atcacggtgg cgcttgtgtc aggcgcggtc gtcgagcgga tgcgcttcgg cgccttcctc
     3661 gtctttgcaa cgctctggtc ggtgctcgtc tacgccgtgc tcgcgcactg ggccttcggc
     3721 ggcggctggc tgatggaggg cgggacgctc gacttcgccg gcggcgtcgc ggtcgagatg
     3781 ggctcgggct tctcggcgct cgcggcggcg ctcgtcgtcg gggcgcgcaa ggactacggc
     3841 cgccaggcgc tgctgccgca caacgcggtc tacgtcgtgc tcggcgccgg cctgctctgg
     3901 ttcggctggt tcggcttcaa cggcgggagc ggcttcggca ccggccaggc gagcatcctc
     3961 gccttcacga acacgctgct cgcaccggcc gcgacgctgg tcgtctggtt cgtgctcgac
     4021 ctgatccgcg gtcgcaaggt gaccgcgatc ggcgccgcga cggcgatcat cgtcggctgc
     4081 gtcgtgatca ccccggcggg cggcttcgtc agcccaggct gggcgctggc gctcggcgcg
     4141 atcgcggcac tgccgtgcta cgcgatgatc gtctggcgcc cgcgcacgcg cgtcgacgag
     4201 acgctcgacg tgctcgccgc ccacggcgtc gcgggcttca gcggcattct gctgctcggg
     4261 atcgtcgccc aggcgtcatg gaacggagtc tcggacggcc tgctctacgg caacgccgac
     4321 cagttcctct ggcaggcgat cgccgccgcg gcgactcctg cctacgcctt catcgcgacc
     4381 ttcgtcctgc tcaagctgat cggtgccgtc atgccactgc gcgccagcca tcacgacgag
     4441 gcgatcggga tggacatcgt ccagcacggc gaggaggcct acgtgaccgg cgagggcgcg
     4501 atcctgatct cctcgcccga tggcgaggag cccggcgtcc ctgtcgcgaa cccgatctag
     4561 cggacgccgg ccgggatcgg cgtccgctcg gcgctcgccg cgccgctgcc gggatcgatc
     4621 tcgaccgtgt ctgcggcgag cggctcgaag cggtcgccgg tcgcgtaggt cagcacggcg
     4681 agcccgagcg gctggtgctc gtcgaggttg cccgccccgc ctccgccggc cgtgccgccg
     4741 ttgagctcga cgaggttgtc ggagatcctc agcgccggtt ggtgggtgtg cccgctgagc
     4801 agcacgagcg gccctcgcgg cgggtcggcg cgcagctcgg cgaccgcgag ccgggcgagc
     4861 ttcggttggt ggaccatcgc cacgtcgatc ttcccggcga gtggaagcag ccagtcggcg
     4921 aaccgccgct ggcggtcgat gtcgtccccg tcgtcgtcgt agcggtcgcg gtagccgtca
     4981 gcgcgacgtc gctggtcggg gtcgccgtag ccggcaacac gcagtccggc gacgcgcacg
     5041 acgaccggtc cgagcgagcc gtcggcgcgc agccggccgc gctcggtcag gacgaccgcg
     5101 cccgcggcgg cgagccggcg tgcgagcagg tcggagtcgt ggttgccggt cacgaacacc
     5161 agccggctgc cgattcgagc cacccgccgc gtcagggcga cctcgaacgg cgagccgttg
     5221 ctcgtgaggt cgccggcgaa gaacagcggc ccttcgccga cggcgcgctc gagcgtcggc
     5281 agtgcgagca ggttgctgtg caggtcggag gcgagcgtca gctgccgcgc gtcgccctcg
     5341 cgcacgcggt cggacggctc ggacaccaac cgcgccagcc cgacgagctg ggcgtcgagc
     5401 tcctccgaca gcacggccga ggagcgcgtc gcctgctcga ccgcgcgcag ggcggcggga
     5461 atgtccgggc cgtgggcgta gtagtcgggg tcgcccaagt ctccgcgcgg tggcagcagc
     5521 agggcgatcg cagcgcagca gacgagggcg gtcgccgccg ccgtcgccac gagtggcgcc
     5581 agcggcgtcc gccggccgcg cagtgcgagc gcgaccagga cccccgcggc gagcgcagcc
     5641 accgccacga cggcgagcag gaggcgcagg taggcggcga tcgcatcgcg tccctcgtcc
     5701 cggacctgct cgagctcggg aacgtcgccg ccggccacgc gctcgagcgc cgcacggtcg
     5761 acggtacgca ggcctaccga cagcctcgcc ggcagccgga cggcgccggg gaagcgcacg
     5821 ccccagtcga ccagaggcat gtagaggtcg agcgcgccct cgtggccggg ttcgaccgag
     5881 atccggacag tgccggtcga cagctcgcgg tcgagctcga agacggcact cgccccgacc
     5941 gcggcgcccg ccgtagcgag cgccacgcag atcgccgcga gtgctcggcg agcgcgcatg
     6001 accgtcaacc tagacggcca tgaccgctcg cctcgactcg tgccgctatc gaggccgaag
     6061 ccgtgcaact acggcagggg gctgacccat agccggcggt cggcgaaggt cgcgctgccg
     6121 gtgccgcagc agacggcgta gcgcagctcg taggtgtggg tccccggcgt ggccaggagc
     6181 ctgaccggcg caggtggtcc caacgtcgcg cacaacagcg atcccgctgg cgtgccgcca
     6241 ctgaagtcgt cgccgctgcc tgagcgaagc gagaacagca tcgcgccagg cgggccgcag
     6301 tcggcctggc cggcgacggg gctgccgtcc tcgaacaggg cgacggcgcc gtcgtcgtcg
     6361 gagccgtcgc gcagcgtggc ctgggccgcc acctcgatca gacccgactc cggcaccgtg
     6421 acctcgaccg aggggccgcc gagcgagacg taggtcgtcg agctggtcga gacgtcgggc
     6481 gtctcctggt cgaaggctac gctcgcgtcc cgaccgggtg cgcccgccgg tccgctcggg
     6541 ccgcgcgcac ccgtcgatcc aggcgagccg gtcggtccga tcgcgccgcg cggcccatgc
     6601 ggcccgcggc gcgccttctc gcgtgcgatc cgcttgctct ggcgggcgat ccgcttcgca
     6661 gtgcgcgcat cgcgctgagc cgcgttcgcc ttgcgctcgg cggacttcgc gatccccagg
     6721 gctcgggtga gcatgctgtc gccgttgccg agcgccggcg cagtcagcgc gagggcaagc
     6781 accgcgatca gggccagcgt cagggttcgg cggctcacgt cccgggtcat cggccgctcc
     6841 ccaatgtggc ttgagcctgg ctcggacgcg ttcgacggtt gacccttcgc tagcttcatc
     6901 ggtcgcgatg gcggagttca tctacacgat gcgggagctg acgaaggtcc acccgcccga
     6961 caaggaggtc ctcaaggacg tcacgctcgc ctacctgcgc ggggcgaaga tcggcgtcct
     7021 cggaccaaac ggggccggca agtcgaccct gctgcggatc atggccggcg tcgacacgga
     7081 gttccgcggc gacgcgcagc tcgccgaggg cgcgacggtc gggctgctcg agcaggagcc
     7141 ggcgctcgac ccggccaagg acgtgcgcgg caacgttgag gacggcgtac ggccgctgcg
     7201 cgacctgctt gaccgcttca acgagctcgc cgcgaactac tccgaggaca ctgccgacga
     7261 gttcgcccgg ctccagaagg agatcgacgc ggccgacgcc tggaacctcg acacgctgct
     7321 cgacaccgca atggacgcgc tgcggcttcc gccgggcgac gccgacgtga ccaagctctc
     7381 cggcggcgag cgccgccgcg tcgcgctctg ccggctgctg ctcggccgtc ccgacctgct
     7441 gctgctcgac gagccgacca accacctcga cgccgagtcg gtcgcctggc tcgagcgcca
     7501 cctcgccgac taccccggtg cgatcgtcgc tgtgacccac gaccgctact tcctcgacaa
     7561 cgtcgccggc tggatcctcg agctcgaccg cgggcgggcg ctgccgttcg agggcaacta
     7621 ctcgtcctgg ctcgagcaga agcagaagcg cctcgcccag gaggagaagc aggagtcggc
     7681 gcggcagcgg acgctggcga gcgagctcga gtgggtgcgg atgaacgcgt cctcgcggct
     7741 gacgaagaac aaggcccgcc tcgcgcgcta cgacgcgctg ctcgccgagg agcgcaacgt
     7801 caagctcgac aacgtcgaga tccacatccc cgccggtcag cggctcggcg acgtcgtcgt
     7861 cgaggcggag gggctgagca aaggctacgg cgaccggctg ctgatcgacg acctcagctt
     7921 caagctgccg ccggcgggga tcgtcggcgt gatcggcggc aacggtgccg gcaagaccac
     7981 gctgctgcgg atgatcaccg gcgaggagca gcccgactcc gggacgctgc gcgtcggcga
     8041 gaccgtcgag ctctcctacg tcgaccagtc gcgcgacgcc ctcgatcccg acaagacggt
     8101 ctggcaggag atctccggcg gcgacgacca gctgaaggtg ggggaccgga cgctcaactc
     8161 acgcgcctac gtctcgtcct tcaacttcaa gggctccgac cagcagaaga aggtcggctc
     8221 gctctccggc ggcgagcgca accgcctcca catggcgaag ttgctgcgca ccgccggcaa
     8281 cctgctgctg ctcgacgagc cgtcgaacga cctcgacgtc gacacgctgc gcgccctcga
     8341 ggaggcgctg ctcgcctttc ccggctgcgc cgtcgtcgtc tcgcacgacc gctggttcct
     8401 cgaccgcgtc gccacccacg tgctcgcctt cgagggcgac tccaaggtca cctggttcga
     8461 gggcaacttc gacgcctacg agacctggcg ccgcgagcag ctcggcgagg ccgccgacca
     8521 cccgcaccgc atgacctaca agaagctgat cgcccgccgc tagccgggcc cgacctcgac
     8581 gacgagcgtg atcggcccgt cgttgaccag cgagacctcc attcgcgccc cgaacacccc
     8641 gccctgggcc cccagcctgg cccgcacgcg ctcatagagc ggctcggcga cctcgccggg
     8701 cgctgcctcc ccaaagctcg gccgcgtgcc cttgcgcgcg tcgccgtaga gcgtgaactg
     8761 gctcacgcag agcagctcgc cggcggtgtc ggccacggag cggttcatgc ggccgtcgtc
     8821 atcgggaaag atccgcagcg cgagcagctt ctcggccatc cggtcggcct cggcctcacc
     8881 gtcgtcgcgg tgcactccga gcagcacgag cagccccgcc ccgatccgcg cgaccagctc
     8941 tccctcgacc gtgaccgacg cctcgccgac ccgctggacc accgccctca tcgcgggcga
     9001 tgatgccggg gctggtggcg tcgcgccgcc cggtcagccg actggtggtc tggcggcgca
     9061 agctcgacac cccggcgccg cttcgaggac gtcatgctcg accgctccga cgcaccgatg
     9121 cgactcggcg acgacctcga cgccgcggcg gctacgtgat ggcgctcgat gtcgacctgg
     9181 atcgtctccg cgcgcgcccc cggctgagcc tgagaggatg tggggatgcc cactcctccc
     9241 accggacgca gcgtgaagat cgatcaggac gagcgcggcg gctggtcctc ggaccgtccg
     9301 cgccgcgacg gcgcgcctcc gcgcccggcg gacctctccg tgcgctgccg ggtgctgcgc
     9361 cccgtggagc tgttgcgcta ctcgccaggc agcgtggtga tcgtcgtctc ggcggtgatc
     9421 gcggatggcg agcgcttcgc cgagcggctg atcgaggagc gtggcgtgtt gatgtcaccc
     9481 ggcaaggtgc gcggcctgct cgccggtcgg gtgcccgagg aggagattga gccgcgcgcg
     9541 gcggagctgc tgcacgccgc ggcgctcaag cggatcgagg ctaaggagag cgtggtcgtc
     9601 gtcaccggtg atatcggtgc cgaggagcgc gagcgctacg tgcggcccgc cgccgcccag
     9661 aagcggccgt gtcacatcat tctcgtcgag ccggcgcgtg acgacgtgga cgaggaggac
     9721 cgtcgcacgc tgaacaagtt gcgcaccgcg ctcgacgccg gcgacctcgg cgccgagggc
     9781 ttcaacaccg cgctgcggct cggtccggca tcggccggcg aggtgaagcg aatcgtcttc
     9841 cgcgcaccgc cgagggacga ctagctgtcg tcaaaggctg aaaagcagcc cgcaaagccg
     9901 catttccacg gtgtttgcgg gatcctgcag cgatttttga aataactgct gcaagcaacc
     9961 caaacgtgtg gcatacttca tatcgatccc tagggatcag cgcccccggc cgctccctcc
    10021 aattgcggtc gggggctttt acgttctcag acgtggaagc aaccttcgga ggacgcgaca
    10081 ctcgccgcta gagcgtcgcc aaggcaagcg ggatgacacg cgccgcgccc tgacgacgca
    10141 gctgaccgcc gaccatcgcc atcgtccagc cggacaggcg ctggtcgtcg atcagcaggc
    10201 ccgtcccggg cggcggcgtg gcggtcacgc ggaaggcgcc gcgcacgttg gcggcctgct
    10261 gaacggcgtt ggccatctcg cgctgcggcg gtcgctcgcc cgttcgctcg aggaccgcga
    10321 cgcgcggcac cccgagctcg gcggcgaccc gcgtcgcgag gtcgtcgagg agcccgtcgg
    10381 gcagacgggc cgaagggata gccgtgaccc aggccggctt cgcgccgccg ttacgcacga
    10441 cgtcggccag ggcggccgcg atctcgtcgc cgagccggcc cgccgcgagg ccgcgctcga
    10501 tcgccggcca ccagccgccg tcgccgaccc gagcgagcgc ccagccgctg tcggtgcgcg
    10561 cgtcctccgc gatcttgcgc atcgcgccgg tttcgggatc gggcgccatc ttcttcgcct
    10621 cgagctcgat cggccgcgag cgcaggtggc gctgggcgag ctcgacgagg gcggggtccg
    10681 gtggcgtggc gaagcgggac gcggtgcaga ccgagcagcg cccgcagtcg cgcgggtcgg
    10741 gatcgtcgag ctcctcctgc agcacgcgca tcaggcagcg gccgtcgacg ccgtaggcgg
    10801 ccatcgcctc ctgctcggcg cgccgcaggg cggtcacctg ctcgtagcgg gtgacgtcgt
    10861 aggtccagtc gctgcccggg ttcgggtacc agcgccgtcc gtcgcgcctg accgcgccct
    10921 cgacgtcgag gatcttcagc atcgcgtcga tccggcccat gccaaggttg acgacggcca
    10981 tcagctcgcg tgtcgttcgt cccgcgccac ccgccgccgc cagctcgtcg agcacggccc
    11041 ggacgcgctc gtacggcggg aacgcctgcg cgatgaagaa gtcctggatc ttgcggtcct
    11101 ctccaccgcg cagcagcacg acgtcggcgt ggtcgacgcc gcggccggcg cgaccgacct
    11161 gctggtagta ggagaccacg gaccccggag cctggtagtg cacgacgaac gtcaggtccg
    11221 acttgtcgta gcccatgccg agcgcgctcg tcgcgaccac ggccttgacg tcgttgcgca
    11281 gcagccgctc ctcggtcgcg atcctcagct ccgagtcctg ctcgccgctg taggcgagcg
    11341 ccggcacgcc ctgcgccgag aggaagctcg ccacctgctc agcgtcgcgc ttggtgagcg
    11401 tgtagacgat ccccgatccc ggcagctgcg gcaggttctc gacgagccag gcgagccgct
    11461 ccgcgggccg cggcagctcg agcgcctcga gccgcaggct ggttcgcgcc agcggcccgc
    11521 gatacgagcg caggtcggcg ccgtctgaca gctgctcctc aacgtcggcg acgacacggt
    11581 cgttggcggt cgccgtcgtc cccaggaccg ccacgtcggg ggcgagcgcg tcgagcatgt
    11641 ccttgacgcg ccggtagtcg ggtcggaagt cgtggcccca gtccgatatg cagtgcgcct
    11701 cgtcgatcac cagcagtccg accgaggccg cgaacagcgg cagcatgtcg tcgcgaaagc
    11761 gcgggttgtt gagccgctcc gggctgatca gcagcaggtc aacggtcccc tcgccgagca
    11821 tccgccgcac gtcgtcccac tcgtcctggt tggtgctgtt gaccgtgtgg gcgcgcaacc
    11881 ccagccgctc ggcggccgcg atctggttgc gcatgagagc gagcagtggg ctgacgatca
    11941 gggtcggccc cgctccggcc tcacgcagca gtgccgtcgc cacgaagtac accgccgact
    12001 tcccccatcc ggtccgctgc acgcacagca cccgcgcacg atcctcgacc acgtcgcgga
    12061 tcgcctcgag ctggtcgtcg cggaattcag cggccggatc gcccgtcagc gcccgcagcc
    12121 gctcgagtgc cgcgtcggcg aacggctgcg ctggggcgga ggacgtcacc cgcccatcgt
    12181 gtcaggccgg ccgagcaccc tggacgtccg tcgcaatcct gttacatcga agactatatg
    12241 tgctatgtcg attcaatatc ctgtcgaacg tggagcgacg ggtggacacg agcggcctga
    12301 cgaatgaccc agcatcttga ttgccgcctc gatcagttcg gccgacggtt cgacgaggtc
    12361 gaccgccgtc tcgatcgaat cgacgagcgc attcgtctac tgaccacgtg ggtgacgtgg
    12421 gtcggcggca gcaccatcgg tctgctcatc ggcctcatcg ccgccgcggt gatcgaccac
    12481 ggttagtgac gtgtgcgtac tgtcaccgtg gcgttgcagt cagccccccg ctgtcagaca
    12541 tgctcgggga tggtgcccat gcctccggga ccgtgcaagc cgaacacgcg tcgcccccgt
    12601 agctcagcgg agagagcacc ggtgcgccaa accggaggtc gcaggttcga atcctgccgg
    12661 gggcgcgcta gcatcaccac cagtttggcg caccggagcc ggtctcgacc ggcactgagc
    12721 tacgagcgaa gcccgcgctg tgcgcgggct tcgtgcatct tgggcgcgag gttaggcagg
    12781 ctgtcgctcg atggtcggcg cacacgcggt cctcgactac gaggatttcg accccgctag
    12841 cttcggctag acaggctggt cacgcgcggc gcggtcatac agcacgttgt agtcctcagc
    12901 cagcgcgtcg gagatcggac gccacgctcc actgatgacc tgggcatcta ggtcgtctgt
    12961 ggcggcatct ggctgcttat agaggcgagg caacaggtcc cgcgcgcttc gccaagccgc
    13021 tgccgtggca ggggaccacg gtcgggcgag aaacgtgtcg atctcggagc gctccgggct
    13081 gaagccggcg tccgctcgcg ccgaggcatg ggcaacctca atcgtgacca gttgccagtg
    13141 tccgtagacc tcgtagggca cggcatcgtt gacagccgcc catctgacga ggcgaggtag
    13201 atcccgttcg tagtcgtccg tcaccgcatt gaggtggctg tgatcgatga tcgagtcgcc
    13261 ccaaccctca gcctcgaaga ttgtccttcg acgacgctca actacgtact cctcccacat
    13321 cacccgcttc atcacatcat ctagcccgtc gctatgagcc tcgtcggccg tgcccttggc
    13381 ggcgcccagc gcgcactcaa caatctcatg gcatgcgatg gtgagaacag cggcgtcgcg
    13441 tagccgcgac gcatcgacga cggccacgta ctcgccgttc gtagagcgga aacccatgcc
    13501 gacgccgcgt gcgccttgct gttgtccccg cgcgcgttgt ctccgctccc agcggctgaa
    13561 ctcgtccgtc gaaaggtgtg cgcggagcgt gcttgcgaaa tcgctaccaa tcaggacgcg
    13621 agacggcaac ggtccagacc acggacccgt agatacgagc tctccgaagc gcatgattcg
    13681 gcgtgcgacc tggccactga cgtttccctc gcaaagcatc cgaccagact agggcttcga
    13741 acagatgggg atactgcatt acgcgagcgt caaggctgac gctcaactat ctcaattcga
    13801 tacccgtccg ggtctctgac gaaggcgatc cgtgagccgg tggtgcggcc gccgggccgg
    13861 tagggcggct tctccggctc gatccccgac tgggcgagcc cggcgagcgt tccgtcgaga
    13921 tcgtccacgc cgatcgcgat gtggttgtag ccggttccca ggtcgtagga gtcgacccca
    13981 tggttgtagg tcagctccag tcggggatcc tcaccgggca gtcccatgaa cacgttgatc
    14041 gcctcgtcgc cgatcggcat ccgccgccgc tcctcgaacc cgagcgtctc gtagaactcc
    14101 accgaccgat cgatgtcgcc gatccggtaa caggtgtgca ggaaggacgc catgccttcg
    14161 aggctagccg gcgtacatgc gatcgatctc gtccgcgtac ttgtcgacca cgaccgagcg
    14221 cttgaccttc agcgtcgggg tcagctcgcc ggtctcctgc gagaggtcgt gcggcaggat
    14281 cgtcagcttc ttgacctgct cgacctgggc gtacttcttg ttgacctcgg ccatgtgacg
    14341 ctcgagctcg gcgcggatct cggggctggc ggcgagcgcg gcgacgtcgg tcggcaggtc
    14401 gtgcgccttg gcgtactccg gcccctcctc cgggtcgagc gtgacgagcg cggtcaggta
    14461 gggcaggcgg tcgccaacca tcacgcactg gctcacgagc gggtgctgct tcatgtcgcc
    14521 ctcgaagttc gccggcgtga tgttcttgcc gcccgcggtg atgatgatgt ccttcttgcg
    14581 gccggtgatc cgcacgaagc cgtcgctgtc gatctcgccg agatcgccgg tgtgcagcca
    14641 gctgtcgatc agcgtctcac gggtcgcgtc gtccttcttg tggtagccct ggaagacgtt
    14701 cgggcccttg acgcagatct cgccgtcgtc ggcgatcttc acctcgcagc cggggaacgg
    14761 caggccgacg gttccgaact tgtacctatc cggacggttg atcgtggcgg ccgtcgaggt
    14821 ctcggtcatc ccgtagccct cgaggatcag gacgcccgcg gcccagaaga actcgaggat
    14881 ctcgcggttg atcggcgccg cgccggtgac gcagagccgc accctgccac cgaacagggc
    14941 gcggatcttg ctcagcgcca gcttgtcggc gagggcgaac tgcttctcga gcaggaaacc
    15001 gggcgagcgg cccgcccgct cggcctcgcg gtacttgcgg ccggtcgcga tcgaccagtc
    15061 gaggatcttc ttcttcgctc cctcggcggc ggtggccttg gtgtagatct tctcgaagat
    15121 ccgcggcacc gacggcaggt aggtcgggcg gacctcggcg acgtcgtcga tgatcttctt
    15181 cggatcgagc gaccagtagg ccatcgtcga gccgagctcg aatacgccga gctggatcag
    15241 caacgcgaac gagtgggcga gcggcaggaa gaggtagacg acgtcgccgc tcgcgacgac
    15301 gtcctccgac acgaccatgt cgaccatcgc gcggtagttg gcgtgcgaga tcacgcagcc
    15361 cttgggcggg ccggtagtcc cggacgtgta aataaacgtg cagatgtcgt cgccggtcac
    15421 cgacccgtag cgggcggtcc aggcggagtc ctccaccgcg cgctacgggt cggtgacagg
    15481 cgacgacgtc tgcaccttca tctacacgtc cggcacgacc ggtcccccca agggctgcgt
    15541 gatctcacat gcgaactacc gggcgatggt cgacatggtc gtgtcggagg acgtcgtcgc
    15601 gagcggcgac gtcgtctac
//
LOCUS       DATABV010000206         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2798825, whole genome shotgun sequence.
ACCESSION   DATABV010000206 DATABV010000000
VERSION     DATABV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2798825"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3992)
                     /locus_tag="VHF88_09460"
     CDS             complement(<1..3992)
                     /locus_tag="VHF88_09460"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX2162031.1"
                     /translation="MAAPGTTVQITMPAMGESVTEGVVLEWLKQVGDRVEVDEGLVEI
                     STDKVDAEVPSPAAGVLTAINVQPDETVTVGTILGEIEVGEGGAPPGNAPGGGADGEA
                     GASDGAAAGSGNGAAQAAAPASDPVAVDGDATPVAARMAADLGVDIAGVKGSGPRGRV
                     TKDDVLAAVANAVKGNGGAATPASAPAPPDGAAAKQIRGPAATLVRFMEDSRSIPTAT
                     SFRTLAVDTLAARRATLKGAGKKLSFTHLIAWAIVRATDDMPVMADSFAAVDGKPHRV
                     TPEAVSLGLAVDVERKDGTRSLVVPVIHDASKLSFPDFVAAYDELVVGARDNTLQPDA
                     YQGANITLTNPGGIGTVASVPRLMPGQGTIVATGAIGYPPGLAQADPRKLAELGVEKV
                     MTMTSTYDHRVIQGAESGQFLKRVDELLQGADGFYDSLFEALGIDAAQVEAGPPSPTV
                     TSADPVPASTTPAVSDVPDEALMQAVQAATSIIKAHRVHGHLAAHLDPLGSAPRGDPA
                     LDPESVNLTQEMMERIPASVLRVAVPGKTFADALPHLRETYTGTIAYEVEHIAGHNRR
                     VWLRENIESGAHRTPLTAEQKRRLLERLSQVEALEGYLHKAFLGKKQFSIEGLDMLVP
                     MLDATIELCADSGAREAVIGMAHRGRLNVLAHTVGQPYGSILVEFEGEQTLSADTAAP
                     EGGTGDVKYHYGASGTYKTHDGKGVQVTLSPNPSHLEYVNPVIEGRARADQTSRNGRE
                     MTLDPSLVVPILIHGDAAFPGQGVVAETLNLQALAGYTTGGTVHIIANNQLGFTTDPT
                     DARSTRWASDLAKGFDVPIIHVNADDVEACIAAVRLAVSFRNEFGRDALIDLIGYRRF
                     GHNETDEPAYTQPQMYEKIKSHPPVRAIYAKRLIEEGVVTAEEAEQLAADAYEKVASA
                     HTELKDTMAGPPDTGSLELDTTMSREPKTTLPADLLRSLNEQIVKLPDDFEVHRKLKP
                     FLKKRVEAAEPGGRVDWAHAEALAFASLLALGVPIRLTGQDSERGTFSQRHLVLHDAE
                     SGDGYTPLAHLQSAAAPFELHNSPLSETACLGFEYGYSIQCPDALVLWEAQFGDFVNS
                     GQVIIDQFMTSGLAKWGQTSRLTLLLPHGYEGSGPEHSSGRVERFLQACAEGNIRVAN
                     ATTPAQYFHLLRRQALVSKARPLVVMTPKSLLRLPAATSAFEELTDGEFQRVIDDPLF
                     AGGAGGNGSAPKDRDKVTKLVLCSGKVYYDIVANEQREEADHIAVARVEMLYPFPEEQ
                     LKELMDSYPNLERVVWVQEEPRNMGPRAYMRRRMAGILPEHLHYYYVGRQLRAAQSEG
                     YTAAHRKEQAR"
     gene            4037..>4434
                     /locus_tag="VHF88_09465"
     CDS             4037..>4434
                     /locus_tag="VHF88_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2162032.1"
                     /translation="MSDGRRKRELARADRVLPGVWRLRLPLPWLGVPHVNAYAIAAGN
                     GVVLFDTGMHEPGAFAQLERALHQAGLGVEHIRLVVCTHAHSDHYGLAAPICEAAGCE
                     LWMHPDHRHMTAAAQEPTVALERRIEVARQ"
BASE COUNT          559 a         1502 c         1673 g          700 t
ORIGIN      
        1 cgcgcctgct ccttgcggtg cgcggcggtg tagccctcgc tctgggcggc gcggagctgg
       61 cggccgacgt agtagtagtg gaggtgctcg ggaaggatgc cggccatgcg gcggcgcatg
      121 taggcgcgcg ggcccatgtt gcgcggctcc tcctggaccc agacgacccg ctcgaggttc
      181 gggtagctgt ccatcagctc cttgagctgc tcctccggga acggatagag catctcgacg
      241 cgcgcgacgg cgatgtggtc ggcctcctcg cgctgctcgt tggcgacgat gtcgtagtag
      301 accttgccgg agcagaggac gagcttggtg accttgtcgc ggtccttcgg cgccgagccg
      361 ttgccgcccg cgccgccggc gaacagcggg tcgtcgatca cgcgctggaa ctcgccgtcg
      421 gtgagctcct cgaacgccga cgtggcggct ggcaggcgca gcaggctctt cggcgtcatc
      481 acgaccagcg gccgcgcctt cgagaccagc gcctggcggc gcagcaggtg gaagtactgc
      541 gccggcgtcg tggcgttcgc gacgcggatg ttgccctcgg cacaggcctg caggaagcgc
      601 tcgacgcgac cggaggagtg ctcgggcccc gagccctcgt agccgtgcgg cagcagcagc
      661 gtcagccgcg acgtctggcc ccacttcgcc agcccggagg tcatgaactg gtcgatgatc
      721 acttggcccg agttgacgaa gtcgccgaac tgggcctccc acagcacgag cgcgtccggg
      781 cactggatcg agtagccgta ctcgaagccg aggcaggcgg tctccgacag cgggctgttg
      841 tgcagctcga acggcgcggc ggcgctctgc aggtgcgcca gcggggtgta gccgtcgccg
      901 ctctcggcgt cgtgcaggac gaggtggcgc tggctgaacg tgccgcgctc ggagtcctgg
      961 ccggtcaggc ggatcggcac gccgagcgca agcagcgagg cgaaggcgag cgcctcggcg
     1021 tgcgcccagt cgacgcggcc gcccggctcc gcggcctcga cgcgcttctt caggaagggc
     1081 ttgagcttgc ggtggacctc gaagtcgtcg ggcagtttga cgatctgctc gttgagcgag
     1141 cgcagcaggt ccgcggggag ggtcgtcttc ggctcacggc tcatcgtcgt gtcgagctcg
     1201 agtgagccgg tgtcgggcgg gccggccatc gtgtccttga gctcggtgtg cgcgctcgcg
     1261 accttctcgt aggcgtcggc ggccagctgc tcggcctcct cggcggtgac gacgccctcc
     1321 tcgatcagcc gcttcgcgta gatcgcgcgc accggcgggt gcgacttgat cttctcgtac
     1381 atctgcggct gggtgtaggc gggctcgtcg gtctcgttgt ggccgaagcg gcggtagccg
     1441 atcaggtcga tcagcgcgtc gcggccgaac tcgttgcgga agctgacggc gagccggacg
     1501 gcggcgatgc aggcctcgac gtcgtcggcg ttgacgtgga tgatcggcac gtcgaagccc
     1561 ttggcgaggt ccgacgccca gcgcgtcgag cgcgcgtcgg tcgggtcggt cgtgaagccg
     1621 agctggttgt tggcgatgat gtggacggtg ccgccggtcg tgtagcccgc cagcgcctgc
     1681 aggttgagcg tctcggcgac gactccctgg ccggggaagg cggcgtcgcc gtggatcagg
     1741 atcggcacga cgaggctcgg gtcgagcgtc atctcgcggc cgttgcggct cgtctggtcg
     1801 gcgcgcgcgc ggccctcgat caccggattg acgtactcga ggtggctcgg gttcggcgac
     1861 agcgtcacct ggacgccctt gccgtcatgc gtcttgtagg tcccggacgc gccgtagtgg
     1921 tacttgacgt cgccggtgcc gccctcgggc gccgcggtgt cggccgacag cgtctgctcg
     1981 ccctcgaact cgacgaggat cgatccgtag ggctggccga cggtgtgcgc caggacgttg
     2041 agccggccgc ggtgggccat cccgatcacc gcctcgcggg cgccgctgtc ggcgcagagc
     2101 tcgatcgtcg cgtcgagcat cggcacgagc atgtcgaggc cctcgatcga gaactgcttc
     2161 ttgccgagga acgccttgtg caggtagccc tcgagcgcct cgacctgcga cagccgctcg
     2221 agcagccggc gcttctgctc ggccgtcagc ggcgtgcggt gcgcgcccga ctcgatgttc
     2281 tcgcgcagcc agacgcggcg gttgtggccg gcgatgtgct cgacctcgta ggcgatcgtg
     2341 ccggtgtagg tctcgcgcag atggggcagc gcgtcggcga acgtcttgcc gggcaccgcg
     2401 acgcgaagca ccgacgccgg gatgcgctcc atcatctcct gggtcaggtt gaccgactcc
     2461 gggtcgagcg cggggtcgcc gcgcggcgcc gagccgagcg ggtcaaggtg cgcggcgaga
     2521 tggccgtgga cgcggtgggc cttgatgatc gaggtggccg cctggacggc ctgcatcagc
     2581 gcctcgtccg ggacgtcgga gaccgccggg gtggtcgacg ccgggaccgg gtcggccgag
     2641 gtgaccgttg ggctcggcgg tccggcctcg acctgggcgg cgtcgatccc gagcgcctcg
     2701 aagagtgagt cgtagaagcc gtcggcgccc tgcagcagct cgtcgacgcg cttgaggaac
     2761 tggcccgact ccgccccctg gatgacgcgg tggtcgtagg tcgaggtcat cgtcatgacc
     2821 ttttccacgc cgagctcagc gagcttgcgc gggtcggcct gggcgaggcc gggcgggtag
     2881 ccgatcgcgc cggtggcgac gatcgtcccc tggccgggca tcagccgcgg caccgaggcg
     2941 accgtaccga tgccgcccgg gttggtcagc gtgatgttcg cgccctggta ggcgtcgggc
     3001 tggagggtgt tgtcgcgcgc gccgacgacc agctcgtcgt aggcggcgac gaagtcgggg
     3061 aacgacagct tcgaggcgtc gtggatcacg ggcaccacaa gagaccgcgt gccgtccttg
     3121 cgctcgacgt cgacggcgag cccgaggctg accgcctccg gggtgacgcg gtggggcttg
     3181 ccgtcgacgg cggcgaacga gtcggccatc accggcatgt cgtcggtcgc gcggacgatc
     3241 gcccaggcga tcaggtgggt gaacgacagc ttcttgccgg cgcccttgag cgtcgcgcgg
     3301 cgggccgcga gggtgtcgac ggcgagcgtg cggaagctcg tcgcggtcgg gatcgagcgg
     3361 ctgtcctcca tgaagcggac gagcgtcgcc gcgggcccgc ggatctgctt cgcggcggcg
     3421 ccgtcgggtg gggccggggc actcgccggc gtggcggcgc cgccgttgcc cttgacggcg
     3481 ttcgcgacgg ccgccagcac gtcgtccttg gtgactcggc cgcgcgggcc gctgcccttg
     3541 acgccggcga tgtcgacgcc gaggtcggcc gccatgcggg cggcgaccgg tgtcgcgtcg
     3601 ccgtcgaccg ccacagggtc ggaggcggga gcggcggcct gtgccgcacc gttgccggag
     3661 cccgcggcgg cgccgtcgct ggcgccggcc tccccgtcgg caccgccgcc cggggcgttc
     3721 cccggcggtg cgccgccctc gccgacctcg atctcgccaa ggatggtgcc gacggtcaca
     3781 gtctcgtcgg gctggacgtt gatcgcagtc agcacgccgg ccgcaggaga cgggacctct
     3841 gcgtcgacct tgtcggtcga gatctcgacg aggccctcgt cgacctcgac ccggtccccg
     3901 acctgcttca gccactccag caccacgccc tcggtgaccg actcgcccat cgcgggcatc
     3961 gttatctgaa cggtcgttcc gggggcggcc atggccgcaa cccactctat ccggcgcaag
     4021 atagagtggc ggcgcggtga gcgatggcag acgcaagcgc gaactggcgc gcgccgatcg
     4081 cgtcctgccc ggcgtttggc ggctgcgtct gccgttgccc tggctaggcg tcccgcacgt
     4141 caacgcctat gcgatcgccg ccggcaacgg cgtcgtcctg ttcgacaccg ggatgcacga
     4201 accgggcgcc ttcgcgcagc tcgagcgggc gcttcaccag gccggcctgg gcgtcgagca
     4261 catccggctg gtggtctgca cccacgccca ctcagaccac tacgggctcg ccgcgccgat
     4321 ctgcgaggcg gccggctgcg agctctggat gcaccccgac caccgccaca tgaccgcggc
     4381 ggctcaggag cccacggtag cgctcgagcg caggatcgag gtcgcgcgcc agag
//
LOCUS       DATABV010000207         7450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_68214, whole genome shotgun sequence.
ACCESSION   DATABV010000207 DATABV010000000
VERSION     DATABV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68214"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VHF88_09470"
     CDS             complement(<1..766)
                     /locus_tag="VHF88_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HEX2162033.1"
                     /translation="MLGRRGRMLVAEPFFDSHGQSVEVARGKAGEGDVVLVGVGKRGA
                     RILRKIGKPSVARDVVEALMLDRGLRRSFPRRSETEAIEVASDPPVGGGRTDLTGLPT
                     FTIDPDSARDFDDAISAEQLEDGLVRVWVHIADVTAFVRPGGALDREAQRRGTSTYVP
                     GAVEPMLPEQLSNGACSLRPGEDRLAVTVELDIAGVDVRRVAFHRSLIRSDARLTYGQ
                     VDEVFAGRARAEAPWGEPLAAAREVARALRERREQRR"
     gene            complement(864..2045)
                     /locus_tag="VHF88_09475"
     CDS             complement(864..2045)
                     /locus_tag="VHF88_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX2162034.1"
                     /translation="MGALLKAFAVVLAFTAVLVGGIWLGGHPETLPDPLRDGLVADDR
                     GLRAELQDAIEDDFYKQVDDSQIEQGSLKGMVEGLDDRFSSYLTPKETREFEQSVQGR
                     FEGVGMNVDQDRRGLRVLNVFEGSPAERGGIKKGDFITEVDGRSIAGMSADVATARIK
                     GPAGTRVALQVVDPESFEPRTVRLKRERIEIPITTGRIVERDGRKLGVVTLSTFSEGA
                     HGLVRKEIDELLGKGAEGILLDLRGNGGGLLNEAVLVASVFIEDGLIVSTKGRTKSER
                     KYDAAGGAIDGDIPVVVLVDRGTASASEIVSGAIKDRKRGTVVGVRTFGKGVFQEVQP
                     LSNGGLLDITVGQYYLPNGENIGNKGVRPTVKAADDPDTERDEALPIALDELVQETSG
                     R"
     gene            complement(2077..2982)
                     /gene="ftsX"
                     /locus_tag="VHF88_09480"
     CDS             complement(2077..2982)
                     /gene="ftsX"
                     /locus_tag="VHF88_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX2162035.1"
                     /translation="MSLPFFLKEAVRGLSRNSAPALAAMLTVLLTALVLGVFIPIVQA
                     TTGTANEVRDRVVVNVYVADDATKGEREQLATALERTANVESVEFVSKAEGLAEAKRK
                     NPEAFAEGTDLLGANPLPDMYRVVPDDPDEVSSIVDRLAPVGADGVREPSLAAIDEVR
                     NKEEDTEKILSATGLVKILTGGLMALLVFASVALVANTLRLSIFARRREVEVMKLVGA
                     TNWFIRWPFVIEGVLVGAMGGVLAVLLLTIAKETFIDPLEARFALLAAPETIDFPLLI
                     AVLLLACIAVSAAGSAITLRRFLRV"
     gene            complement(2985..3815)
                     /gene="ftsE"
                     /locus_tag="VHF88_09485"
     CDS             complement(2985..3815)
                     /gene="ftsE"
                     /locus_tag="VHF88_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX2162036.1"
                     /translation="MAQIRTRRRPDVGPAPQPPTPSGDVPVVELRDVSKVYDGGSVGL
                     EHASLKIGRGEFVFLVGPTGCGKSTCIRLLMKELEASSGDVLIAGKKLNDIDRSKVPY
                     LRRNIGTVFQDYKLLPNRTVYDNVAYSLQVIGEQRDEIRRKVPDILRLVGLSTKLHNY
                     PDELSGGEQQRVSIARAFVNHPPLLLADEPTGNLDPETSIGIMQLIYRINRTGTTVVV
                     ATHDKEMVDKMRRRVIELREGRIIRDEQSGLYRPDESTTEFAIRLRGELGVGAEGHPN
                     "
     gene            complement(3909..4643)
                     /locus_tag="VHF88_09490"
     CDS             complement(3909..4643)
                     /locus_tag="VHF88_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2162037.1"
                     /translation="MNRLYPRPGETTAEDLVATLDLGSRAPEERPYVVVNMVSSLDGR
                     AALDGQTRGLSSGDDRKLFHLLRSAGDALLVGAGTVRTERYGEAVKNDELRGARERHG
                     LDPVQPTVIVSGRLLLPSDLPLLQAPGAPVVVATGAKHELEGVTADVTYERVGDDLRL
                     LLARLRSEHGFRSLVCEGGPTLVSYLLAAGLIDELFLSIAPQLVGGGDEPTIATGRAL
                     PDPAGAELVWLCESGGELFTRWRVRG"
     gene            complement(4640..5680)
                     /gene="nadA"
                     /locus_tag="VHF88_09495"
     CDS             complement(4640..5680)
                     /gene="nadA"
                     /locus_tag="VHF88_09495"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX2162038.1"
                     /translation="MPVQPIPPAGAASTQLLELENVPALKDEVRALAAERDAVILAHN
                     YQLPEVQDVAHFVGDSLGLSRQAAASDEQVIAFCGVHFMAETASVLSPEKTVLLPDLD
                     AGCSLADAITADQLRAWKAQHPGAVVVMYVNTSAEVKAETDYCCTSSNAVQVVEHIWR
                     EHGEDTEILFGPDMWLGAYVERETGRRMQVWDGECHVHAGIRPRDINDMQSAHPDAEF
                     MVHPECGCTTQVMEFVASGDIDPERTHMLSTGGMLDFARDSDADEFIVATETGMLHPL
                     EQENPGKRFIPANRMASCKYMKMITLPKLRDALREMAPVVKVDPAIAERARVPIERMV
                     AIGPGSAQPANA"
     gene            complement(5785..6606)
                     /gene="nadC"
                     /locus_tag="VHF88_09500"
     CDS             complement(5785..6606)
                     /gene="nadC"
                     /locus_tag="VHF88_09500"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX2162039.1"
                     /translation="MRDLIQRALDEDLGAGDLTSRAVVPEGTRASGVVVAKADGVLAG
                     SDIAAAVFESVDRRLEVATRATDGDRVAPGDQLLDVSGDARAILAAERVALNLLGRLS
                     GVATLTARYAAAVAGTGARILDTRKTTPGMRALEKAAVLAGGGVSHRFGLYDAILVKE
                     NHVRVAGGVGEAARRALAGAPDGVVVEVEVESLDELDEVLEAGIGRVLLDNMDPEALR
                     AAVARTARRAQLEASGGIDLDSVRAVAETGVDFISVGALTHSAPALDVSLLFTPS"
     gene            6740..7174
                     /locus_tag="VHF88_09505"
     CDS             6740..7174
                     /locus_tag="VHF88_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162040.1"
                     /translation="MRLSTKCLAAPAALLAALAVAVPASSIATPVADDGASAAQRSKD
                     PRDRGDRGDRTSPQCRGLARREEIAQANLSQDQAKLRRAKANLTKARNAVKKTTPGTK
                     ANKKAKAKVAKAKKKVRNAKAAVKDGKATLKNAQRQAQARGC"
     gene            complement(7187..>7450)
                     /locus_tag="VHF88_09510"
     CDS             complement(7187..>7450)
                     /locus_tag="VHF88_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX2162041.1"
                     /translation="MRMLRAKLIEREEQRRQEEIAKEKGEAQDVGWGSQIRSYVLHPY
                     TMVKDHRTNVEIGNAQGVLDGDLDELVRAELLRRAGAASNGAG"
BASE COUNT         1104 a         2664 c         2590 g         1092 t
ORIGIN      
        1 cgcggcgctg ctcgcggcgc tcgcgcaagg cgcgcgctac ctcgcgcgcc gcggcgagcg
       61 gctcgcccca cggggcctcg gcgcgggcgc gccccgcgaa gacctcgtcg acctggccgt
      121 acgtcagcct ggcgtccgag cggatcagcg agcggtggaa ggcgacgcga cggacgtcga
      181 ccccggcgat gtcgagctcg accgtgaccg cgagccgatc ctcccccggc cgcagcgagc
      241 acgcgccgtt gctgagctgc tcgggcagca tcggctcgac ggcgcccggc acgtaggtgc
      301 tggtgccgcg ccgctgcgcc tcgcggtcga gcgccccgcc cggcctgacg aacgccgtca
      361 cgtcggcgat gtgcacccag acccgcacaa gcccgtcctc gagctgctcg gccgagatag
      421 cgtcgtcgaa atcgcgcgcg ctgtcgggat cgatcgtgaa cgtcggcagc cctgtcaggt
      481 ccgtgcgccc gccaccgacc ggtggatcgc tggctacttc gatcgcctcc gtctccgagc
      541 gtcgagggaa gctccgtcgc aggccgcgat ccagcatcag cgcttccacg acatcgcgcg
      601 caaccgacgg cttcccgatc ttccgcaaga tccgagcgcc gcgcttgccc acgccgacca
      661 gaacgacatc gccctccccc gccttcccgc gcgccacctc gacgctctgg ccatgggagt
      721 cgaagaacgg ctcggcgacc agcatccgcc cgcgccggcc gagcacgccg acgaagggcg
      781 ccagcggacg accagcgcgt ccgccgaggc cccggccacc gggcttccgc cccggggggc
      841 ccgggggcgc gagccccccg acctcatcgg ccggaggtct cttgcaccag ctcgtcgagc
      901 gcgatcggca acgcctcgtc ccgctccgtg tccgggtcgt cagctgcctt gaccgtaggc
      961 ctcactccct tgttgccgat gttctcaccg ttcggcaggt agtactggcc gacggtgatg
     1021 tcgagcagcc cgccgttgga gagcggctgg acctcctgga agacgccctt gccaaaggtt
     1081 cggacgccga cgaccgtgcc gcgcttgcga tccttgatcg cgcccgagac gatctccgag
     1141 gccgacgccg tgccgcggtc gaccagcacc acgaccggga tgtcgccgtc gatcgcccca
     1201 ccggcggcgt cgtacttgcg ctcggacttc gtgcgcccct tcgtcgagac gatcaggccg
     1261 tcctcgatga acaccgacgc gacgagcacc gcctcgttga gcagtccgcc gccgttgccg
     1321 cgcaggtcga gcaggatgcc ctcggcgccc ttgccgagca gctcgtcgat ctccttgcgt
     1381 acgagtccgt gggcgccctc gctgaacgtg ctgagcgtga cgacgccgag cttgcggccg
     1441 tcgcgctcga cgatccgccc ggtggtgatc ggaatctcga tccgctcgcg cttcagtcgc
     1501 accgtgcgcg gctcgaagct ctcggggtcg accacctgga gcgccacccg cgtgccggcc
     1561 ggacccttga tccgtgccgt cgcgacgtcg gccgacatcc cggcgatcga gcgcccgtcg
     1621 acctcggtga tgaagtcgcc cttcttaatc ccgccgcgct cggccggcga gccctcgaag
     1681 acgttgagca cgcgcagccc gcgcctgtcc tggtctacgt tcatcccgac gccctcgaag
     1741 cggccctgca ccgactgctc gaactcgcgc gtctccttcg gcgtcaggta gctcgagaag
     1801 cggtcgtcga ggccctcgac catccccttg agcgacccct gttcgatctg gctgtcgtcg
     1861 acctgcttgt agaaatcgtc ctcgatcgca tcctggagct cggcacgcag cccgcgatcg
     1921 tcggcgacga gcccgtcgcg cagcggatcg ggcagcgtct cgggatggcc gccgagccag
     1981 atgccgccga cgagcaccgc cgtgaaggcg aggacgaccg cgaatgcctt gaggagagcg
     2041 cccacgagga cggtcaagag tagtcgccgc gtccggtcag acgcgcagga agcggcgcag
     2101 cgtgatcgcc gagccggcgg ccgagaccgc gatgcaggcc agcagcagca ctgctatcag
     2161 cagcgggaag tcgatcgtct cgggcgccgc gagcagcgcg aagcgagcct cgagcgggtc
     2221 gatgaacgtc tccttggcga tcgtcagcag cagcacggcg agcaccccgc ccatcgcgcc
     2281 gacgaggacg ccctcgatca cgaacggcca gcggatgaac cagttggtcg cgccgacgag
     2341 cttcatcacc tcgacctcgc gccggcgggc gaagatcgag aggcgcagcg tgttggcgac
     2401 cagcgctacc gaagcgaaga cgagcagcgc catcaggccg ccggtgagga tcttcaccag
     2461 gccggtcgcc gaaaggatct tctcggtgtc ctcctccttg ttgcgcacct cgtcgatcgc
     2521 cgcgagcgac ggctcgcgga cgccgtccgc gccgacgggc gcgagacggt cgacgatcga
     2581 ggagacctcg tccgggtcgt cgggcacgac gcgatacatg tccggcagcg ggttcgcgcc
     2641 gagcaggtcg gtgccctcgg cgaacgcctc ggggttcttg cgcttcgcct cggcgagccc
     2701 ctcggccttc gagacgaact cgaccgactc gacgttcgcc gtccgctcga gcgccgtcgc
     2761 gagctgctcg cgctcgccct tggtggcgtc gtcggcgacg tagacgttga cgacgacccg
     2821 gtcgcgcacc tcgttcgcgg ttcccgtcgt cgcctggacg atcgggatga agacgccgag
     2881 cacgagcgcg gtcagcagca cggtcagcat cgcggcgagc gccggggccg agttgcgcga
     2941 gagaccgcgg acggcctcct tgaggaagaa cgggaggctc atcgctagtt cgggtggccc
     3001 tcggcgccga cgcccagctc gccgcgcagc cggatcgcga actcggtcgt ggactcgtcc
     3061 ggacggtaga ggccggactg ctcgtcgcgg atgattcgcc cctcgcgcag ctcgatcacg
     3121 cgccggcgca tcttgtcgac catctccttg tcgtgggtcg ccacgacgac cgtcgtcccg
     3181 gtcctgttga tccggtagat cagctgcatg atcccgatcg aggtctcggg gtcgaggttg
     3241 ccggtcggct cgtcggccag cagcagcggc gggtggttga cgaacgcgcg cgcgatcgat
     3301 acgcgctgct gctcgccgcc cgacagctcg tcggggtagt tgtgcagctt ggtcgagagc
     3361 ccgacgaggc gcaggatgtc cggcaccttg cggcggatct cgtcgcgctg ctcgccgatc
     3421 acctgcagcg agtaggcgac gttgtcgtag acggtccggt tcggcagcag cttgtagtcc
     3481 tggaagaccg tcccgatgtt gcggcgcagg tacggcacct tcgagcgatc gatgtcgttg
     3541 agcttcttgc ccgcgatcag cacgtcgccg ctcgacgcct cgagctcctt catcagcagg
     3601 cggatgcagg tcgacttgcc gcaacccgtc ggaccaacga ggaagacgaa ctcgccgcgg
     3661 ccgatcttta acgacgcgtg ctcgaggccg accgagccgc cgtcgtagac cttcgagacg
     3721 tcacgcagct cgacgacggg gacgtcgccg gagggcgtgg gcggttgtgg cgcgggaccg
     3781 acgtccgggc gcctgcgggt gcgtatctgg gccatgcggg ccacagttga tagcggtccg
     3841 ccgccgccga ctctgcaggt gcaactgcac gtgcagagtt tgcaacgttt gttgcagacg
     3901 cggatcggtc agccgcggac ccgccagcgc gtgaagagct cgccgccgga ctcgcaaagc
     3961 cagacgagct cggcgccggc ggggtccggc aacgcgcggc cggtggcgat cgtcggctca
     4021 tcgccgccgc cgaccagctg gggagcgatc gagaggaaga gctcgtcgat caggcccgcg
     4081 gcgagcaggt aggagacgag cgtgggaccg ccctcgcaga ccagcgagcg gaagccgtgc
     4141 tcggagcgca ggcgagcgag cagcaggcgc aggtcgtcgc cgacgcgctc gtaggtcacg
     4201 tcggccgtaa cgccctcgag ctcgtgcttc gcacccgtag cgacgaccac gggagcgccg
     4261 ggcgcctgca gcagcggcag gtcggacggc agcagcagcc ggcccgacac gatcaccgtc
     4321 ggctggaccg gatcgaggcc gtggcgctcg cgggcgccgc gcagctcgtc gttcttgacc
     4381 gcctcgccgt agcgctcggt ccgcacggtt ccggcaccga ccagcagcgc atcccccgcg
     4441 ctgcgcagca ggtggaacag cttgcggtcg tcaccgctgc tcagtccgcg cgtctggccg
     4501 tcgagcgccg cgcgcccgtc gagcgaggac accatgttca cgacgacgta cggccgctcc
     4561 tcgggtgcgc gcgagccgag gtcgagcgtg gctacgaggt cctcggcggt cgtctcgccc
     4621 ggccgcgggt agaggcggtt caagcgtttg ccggctgggc gctgcccggg ccgatcgcga
     4681 ccatccgctc gatcgggacg cgcgcccgct cggcaatcgc cggatcgacc ttgacgaccg
     4741 gcgccatctc gcgcagggcg tcgcgcagct tcggcagcgt gatcatcttc atgtacttgc
     4801 aggacgccat ccggttcgcc gggatgaagc gcttgccggg gttctcctgc tcgagcgggt
     4861 ggagcatccc ggtctcggtc gcgacgatga actcgtccgc gtcggagtcg cgcgcgaagt
     4921 cgagcatccc gccggtcgag agcatgtggg tgcgctcggg gtcgatgtct cccgaggcga
     4981 cgaactccat cacctgggtc gtgcagccgc actcgggatg gaccatgaac tccgcgtcgg
     5041 ggtgggcgga ctgcatgtcg ttgatgtcgc gcgggcggat gccggcgtgg acgtgacact
     5101 cgccgtccca gacctgcatc cggcggccgg tctcgcgctc cacgtaggcg ccgagccaca
     5161 tgtcggggcc gaacaggatc tcggtgtcct cgccgtgctc gcgccagatg tgctcgacca
     5221 cctggacggc gttcgaggag gtgcagcagt agtcggtctc ggccttgacc tcggcgctcg
     5281 tgttgacgta catcacgacg acggcgccgg gatgctgggc cttccaagcg cgcagctgat
     5341 cggcggtgat cgcgtcggcg agcgagcagc cggcgtccag gtcggggagc agcaccgtct
     5401 tctccggcga gaggaccgac gccgtctcgg ccatgaagtg gacgccgcag aaggcgatga
     5461 cctgctcgtc ggaggcggcc gcctgacgcg acaggccgag cgagtcgccg acgaagtggg
     5521 cgacgtcctg gacctccggc agctggtagt tgtgcgccag gatcaccgcg tcgcgctcgg
     5581 ccgcgagcgc ccggacctcg tccttcagcg cagggacgtt ctccagctcc aacagctgtg
     5641 tggatgcagc tcccgctggg ggaatcggct ggaccggcat gtttcgaacc tctttcggtg
     5701 tcgaaatgaa cgtccgcgcc ccggggagag ggcgcgggac ttgcccaaag tgtagacctc
     5761 caaacctccg cctgcccgcc tgcgctagga cggcgtgaag agcaggctga cgtcgagtgc
     5821 gggcgcggag tgcgtcagcg caccgacgga tatgaagtcg acgccggtct cggcgaccgc
     5881 gcggacgctg tcgaggtcga ttcctcccga ggcctcgagc tgcgcccgtc gggcggttcg
     5941 agcaacggct gcacgcagcg cctcagggtc catgttgtcg agcaggacac ggccgattcc
     6001 ggcttcgagc acttcgtcga gctcgtcgag gctctcgacc tcgacctcga ccaccacgcc
     6061 atccggggcg ccggcgagcg cccgccgcgc cgcctccccc actccgcccg cgacgcggac
     6121 gtggttctcc ttgacgagga tcgcgtcgta gaggccgaag cggtgcgaga cgccgccccc
     6181 ggcaaggacc gctgccttct cgagcgcacg catgcccggc gtcgtcttgc gggtgtcgag
     6241 gattcgcgcg ccggtgccag cgaccgccgc ggcgtaccgg gcggtcaacg tggcgactcc
     6301 cgagagccgg ccgagcaggt tgagcgcgac ccgctcggcg gcgaggattg cgcgtgcgtc
     6361 gccggacacg tcaagcagct ggtcgcctgg cgcgacgcga tcgccgtcgg tggcccgcgt
     6421 cgcgacctcg agccgccggt ctacggactc gaagacggct gccgcgatgt cgcttcccgc
     6481 gagcacgccg tcggccttcg cgaccacgac gccgctcgcg cgggtcccct ccggcaccac
     6541 cgcccgagag gtcaggtcgc cggcgccgag gtcctcgtcg agcgctcgct ggatcaggtc
     6601 gcgcatcgcg gcgactgtac gcgaaaacga ctggtccgga gacgcccgac ggccggtgtg
     6661 gcgccgcgcg atggtggaag tggcgtctcg cggttaggtt ggcgtctcct accccaaccc
     6721 tcggaggact cagcaccaga tgcgactctc gaccaagtgc ctcgccgctc ccgccgcgct
     6781 gcttgcggca ctcgccgtcg ccgtgcccgc cagctcgatc gccacgcccg tcgccgacga
     6841 cggcgccagc gccgcccagc gcagcaagga cccgcgcgac cgcggcgaca gaggcgaccg
     6901 gaccagcccg cagtgtcgcg gcctcgcgcg ccgcgaggag atcgcccagg ccaacctgtc
     6961 tcaggaccag gcgaagctca ggcgcgcgaa ggcgaacctg accaaggcgc gtaacgccgt
     7021 gaagaagacg acgcccggca cgaaggccaa caagaaggcc aaggcgaagg tcgccaaggc
     7081 gaagaagaag gtcaggaacg cgaaggcggc ggtcaaggac ggcaaggcca cgctgaagaa
     7141 cgcccagcgg caggcccagg cacgcggctg ctagccagta cctgctctag ccggcgccgt
     7201 tggaggcggc gccggcacgg cgcagcagct cggcgcgcac gagctcgtcg agatcgccgt
     7261 cgaggacccc ctgggcgttg ccgatctcga cgttcgtgcg gtggtccttg accatcgtgt
     7321 aggggtgcag cacgtaggag cggatctgcg agccccagcc gacgtcctgg gcctcgccct
     7381 tctccttggc gatctcctcc tggcgccgct gctcctcgcg ctcgatcagc ttcgcgcgca
     7441 gcatgcgcat
//
LOCUS       DATABV010000208         7676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1755672, whole genome shotgun sequence.
ACCESSION   DATABV010000208 DATABV010000000
VERSION     DATABV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755672"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      102..271
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            282..1136
                     /locus_tag="VHF88_09515"
     CDS             282..1136
                     /locus_tag="VHF88_09515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2162042.1"
                     /translation="MPSGRSTYVLTALALALIATPATASADRGSSSSAGGVMFVETPK
                     IAKIACVKRCAARKRLQGGSTARISGGSLDQVTTAIFHGSVGSHDDVRAAVKPLNAKR
                     LRAEVPTSAVTGPISLVTTTAVASAPSAPIAILPPLPTDVLTAGDHVFPVAGRHDFGG
                     SGADFGSGRSGHSHQGHDVFARCGTPLVAARGGRIQARKYHSAAGHYVVVDADDSGTD
                     YVYMHLAEPSPFRRGDTVATGQRIGAVGDSGNARGCHLHFELWSAPGYYEGGHPFNPL
                     PALKAWRR"
     gene            complement(1296..2942)
                     /gene="guaA"
                     /locus_tag="VHF88_09520"
     CDS             complement(1296..2942)
                     /gene="guaA"
                     /locus_tag="VHF88_09520"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX2162043.1"
                     /translation="MADAAASSGGVVDAAAAAITGTDPAGAAELDASREVRQDEVVVL
                     DYGGQYSQLIARRVRECGVFSELLPHHVGLDEIRKRRPRGLILSGGPASVYADGAPAL
                     DSELLELEIPVLGICYGMQAMVHSLGGRVESAEVGEFGRSQLTVQEPGRLLAELPTEQ
                     SCWMSHRDTVYEAPPGAVALASSTESPVAAFEDVERGLYGIQFHPEVVHTPYGTQILT
                     RFLEDVCGCDMSWNAASVAEEQIRRIREQVGDGKVICGLSGGVDSSVAALLVHRAIGD
                     QLTCVFVDHGLMRKNEAEQVVSAFRDHFKVPLVAVDAEQRFLARLEGLTEPEAKRKAI
                     GGEFIRVFEEEAAKLGDADYLVQGTLYSDVIESGGGTGAATIKSHHNVGGLPDDLEFE
                     LVEPLRTLFKDEVRRVGIELGLPERLVWRQPFPGPGLGIRIVGGAVTKERLEILRDAD
                     FILQEEIRSAGLYRELWQSFCVLPGDLRTVGVQGDERTYGYVVVVRAVTSDDAMTADW
                     ARLPYDLLERVASRMINEIRQVNRVVLDITSKPPGTIEWE"
     gene            complement(2943..4202)
                     /locus_tag="VHF88_09525"
     CDS             complement(2943..4202)
                     /locus_tag="VHF88_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX2162044.1"
                     /translation="MEIEIGRGKKGRRAYGFDDIAIVPSRRTRDPDDIDITWTLGNYR
                     FELPLLASAMDGVVSPRTAGEIGRLGGLAVLNLEGVFCRYEDAEEQLERISGFDKDEA
                     TAEMQRIYREPVKQELIAKRIREIKDMGVVCAASLTPQRVRDYYETTIEAGIDVLVIQ
                     GTVISAEHVSTDESRPPLNLKEFIREEVPPDVPVVVGGCASYSTGLHLMRTGAAAVLV
                     GVGPGAACTTRGMLGIGVPQATAIADVAAARSQHMLETGEYVKVIADGGMRNGGDISK
                     AFACGADAVMIGSPLARAYEAPGHGYHWGMATFHPDLPRGARVQTTQNGTLEQILLGP
                     AHENDGTFNLMGSLRTSMATCGYEDIPSFHRAEVMVAPSLQTEGKQLQRDQAIGMGAQ
                     SAAKAAPAADGVTNGSGPVHADPALVE"
     gene            complement(4347..5321)
                     /locus_tag="VHF88_09530"
     CDS             complement(4347..5321)
                     /locus_tag="VHF88_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX2162045.1"
                     /translation="MIITVTLNAAIDKTLAVPSFRLGHRHRAVEQASMPGGKGVNVAR
                     ALKSLGRPVIATGFAGGHTGSRIIEQLTDEAILSDFVRIREESRTSTAVVDPTSGEQT
                     EINERGPSVSEAELALFVDKLLYLAKGAALCVFSGSLPRGVDAGLYARLVEELRRVGV
                     TTVLDCEGEPLRLAVRAQPSVIAPNENEAEELVGHEFADDEDRRTALPEMVDLGAREA
                     IMTLADGCLALHGDESDATRPRRLTRATLEPLEPVSSVGSGDAFLAGYVAGRYDGLPD
                     DECLRFGVACGAESTQHFGAGVLDPREVDRLSTAVELETLEAPTLQPG"
     gene            complement(5318..6598)
                     /locus_tag="VHF88_09535"
     CDS             complement(5318..6598)
                     /locus_tag="VHF88_09535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX2162046.1"
                     /translation="MIVRRLAGVAAAVALAWSVVPAASGQAPQRPSLEGADAAILVDA
                     GDGTPILEKEPTQRRSIASTTKLMTALLTLERAPLDAVYAAADYQAAPAESKIDLRPG
                     ERMRVSDLLEALMLESANDAAATLAEGVSGSREAFVAEMNARAGELGLEDTSYANPIG
                     LDDPQNYSSARDLATLARRLLRDRRFARIVDSPSATLESGAEPRVVGNRNDLIASVPA
                     VDGIKTGYTSEAGYVLVGSATGSNGAQVVSVVLGEPSEAARDADTLALLRYGLAQYRR
                     VPLLRKGRAVARADVAHHDETVALVPRRALAVTARRDQRLEQRVRAPEELAGEIAAGT
                     PVGTVVVRRDGEVVERVALLTAKRVPGAGPLRRVVASLGPPLTALALLVIVSVTGAVG
                     MQMRRRHRHRRAATRRRERERVRARDQQDPAGKP"
     gene            complement(6595..6933)
                     /locus_tag="VHF88_09540"
     CDS             complement(6595..6933)
                     /locus_tag="VHF88_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX2162047.1"
                     /translation="MPTHSSRSSARILAPAAIAVCALAVLVVIGSSLSGGGSSGGDPS
                     VSQPASQQTAGTAERPRPRQRANYTIKAGDTLGGIAEQTGVPVETLQELNPELDPQAL
                     IAGQKIKLRE"
     gene            complement(6963..>7676)
                     /gene="pyrF"
                     /locus_tag="VHF88_09545"
     CDS             complement(6963..>7676)
                     /gene="pyrF"
                     /locus_tag="VHF88_09545"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX2162048.1"
                     /translation="PACVAVKPQLACFERLGAPGWAALEATRDVARAHGLLFLADGKR
                     GDVPVTARAYAQALVGSTDGPFGTVEGLRADAFTANPLLGGDAIAPLVEAAAAADAGC
                     FLLVNTSNPGAADLQDLDADGRPLHERLAQLADVFGADQLGDCGLSLVGAVCGATRPE
                     RLGRLRELMPHAIFLLPGVGAQGGSVDALGPAFAPHPAAGLVAASRSIVNAHADRGGD
                     PASAAAASAEELRALAWRA"
BASE COUNT         1048 a         2809 c         2744 g         1075 t
ORIGIN      
        1 cgggggctcg cgcccccgga cccccgaggt tgcgtggccg gtcggcgtgg ggcgcgagcc
       61 ccggtgtgat tgccgccgag cgatgggcta ttctgccgcg cagttcgagt cagccgagtc
      121 ccctgcggtc cagccggagg ggaacggggg aaccaatcga gcgtcgtcaa ggcgctcgag
      181 gggcgaatcg aaggcgcaca gtcgccgccg tagcgccgct ctttcggcgc gagcccgtca
      241 gctaaccccg taggcccaag aaagagaggc agtacgaacc agtgccttcg ggtcgatcaa
      301 cctacgtcct gaccgccctc gcgctcgccc tgatcgcgac tccggcgacc gccagcgcgg
      361 accgcgggtc gagctcctcc gccggcggcg tgatgttcgt cgagacgccg aagatcgcca
      421 agatcgcctg cgtcaagcgc tgcgccgccc gcaagcggct gcagggcggc agcaccgccc
      481 ggatctccgg cggctcgctc gaccaggtga ccaccgcgat cttccacggc tccgtaggca
      541 gccacgacga cgtccgcgcc gccgtcaagc cgctcaacgc caagcgcctg cgcgccgagg
      601 tcccgaccag cgccgtcacc ggcccgatct cgctggtgac gaccaccgcc gtcgcctcgg
      661 caccgagcgc cccgatcgcg atcctcccgc cgctgcccac ggacgtcctg acagccggcg
      721 accacgtctt ccccgtcgcc ggccgccacg acttcggcgg ctccggagcc gacttcggca
      781 gcggccgttc cggccacagc caccagggcc acgacgtctt cgcgcgctgc ggcaccccgc
      841 tcgtcgccgc ccgcggcggc cggatccagg cccgcaagta ccactccgca gcgggccact
      901 acgtcgtcgt cgacgccgac gacagcggca ccgactacgt ctacatgcac cttgccgaac
      961 ccagcccctt ccgccgcggc gacaccgtcg ccaccggcca gcgaatcggc gccgtcggcg
     1021 actccggcaa cgcccgcggc tgccacctcc acttcgagct ctggtcagcc cctggctact
     1081 acgaaggcgg ccatcccttc aaccccctcc ccgccctcaa ggcctggcgg cggtaggtcg
     1141 gggtccatcg ggctagggct ctggccgctc tgcccgcggg tcctgtctcc gattttggtt
     1201 cacgtgctcg ttactgcgcg cgcgaacgca aaatcgtcac cacccgcgga ggcagacccg
     1261 cggcccccac ggccgatccc cctagcgggt agcgcctact cccactcgat agtgcccggt
     1321 ggcttggacg tgatgtccaa gacgacccgg ttgacctggc ggatctcgtt gatcatccgc
     1381 gaggcgaccc gctcgagcag gtcgtagggc aggcgggccc agtccgcggt catcgcgtcg
     1441 tcgctggtga cggcgcggac tacgacgacg tagccatagg tgcgctcgtc gccctggact
     1501 ccgacggtgc gcaggtcgcc gggcagcacg cagaacgatt gccagagctc ccggtagagg
     1561 cccgcggagc ggatctcctc ctggaggatg aagtcggcgt cgcgcaggat ctcgaggcgc
     1621 tccttggtca ccgcgccgcc gacgatccgg atgccgaggc cggggccggg gaatggctgg
     1681 cgccagacga gccgctctgg caggccgagc tcgatgccga ccctgcggac ctcgtccttg
     1741 aagagcgtgc gcagcggctc gacgagctcg aactcgaggt cgtcgggcag cccgccgacg
     1801 ttgtggtgcg acttgatcgt ggcggcgccg gtgccgccgc cggactcgat cacgtcggag
     1861 tagagcgtgc cctggacgag gtagtcggcg tcgccgagct tggcggcctc ctcctcgaag
     1921 acgcggatga actcgccgcc gatcgccttg cgcttggcct ccggctcggt cagcccctcg
     1981 agccgggcga ggaagcgctg ctcggcgtcg acggcgacga gcggcacctt gaagtggtcg
     2041 cggaacgcgc tgacgacctg ctcggcctcg ttcttgcgca tcagcccgtg gtcgacgaag
     2101 acgcaggtca gctggtcgcc gatcgcgcgg tggacgagca gcgcggcgac cgacgagtcg
     2161 acgccgcccg agaggccgca gatcaccttg ccgtcgccga cctgctcgcg gattcggcgg
     2221 atctgctcct cggcgaccga cgcagcgttc caggacatgt cgcagccgca gacgtcctcg
     2281 aggaagcggg tcaggatctg cgtgccgtag ggggtgtgga cgacctccgg atggaactgg
     2341 atcccgtaga gcccgcgctc gacgtcctcg aaggcggcga ccggcgactc cgtcgacgag
     2401 gcgagcgcca ccgcccccgg cggcgcctcg tagaccgtgt cgcggtggct catccagcag
     2461 ctctgctcgg tgggcagctc ggcgagcagc cggcccggct cctggacggt cagctgcgag
     2521 cgaccgaact cccccacctc ggcgctctcg acgcggccgc cgaggctgtg caccattgcc
     2581 tgcatgccgt agcagatgcc gagcaccggg atctccagct cgagcagctc ggagtcgagg
     2641 gccggagcgc cgtcggcgta gaccgaggcg ggaccgcccg acaggatcag gccgcgcggc
     2701 cggcgcttgc ggatctcgtc gaggccgaca tggtgcggca gcagctcgct gaagacgccg
     2761 cactcgcgca cgcgacgggc gatcagctgc gagtactggc cgccgtagtc gagcacgacg
     2821 acctcgtcct ggcggacctc gcgcgaggcg tcgagctctg cggcgccggc gggatcggtg
     2881 ccggtgatcg ccgcggcagc ggcgtcgacg accccgccgg aggacgcggc cgcgtcggcc
     2941 acctactcca ccagggcggg gtcggcatgc acggggcccg acccattcgt gaccccgtcg
     3001 gccgccggcg cggccttggc ggccgactgg gcgcccatgc cgatcgcctg gtcgcgctgg
     3061 agctgcttgc cctccgtctg cagcgacggg gcgaccatca cctcggcgcg gtggaaggac
     3121 gggatgtcct cgtagccgca ggtcgccatc gacgtgcgca ggctgcccat caggttgaag
     3181 gtgccgtcgt tctcgtgcgc cgggccgagc aggatctgct cgagcgtgcc gttctgggtc
     3241 gtctggacgc gcgcgccgcg cggcaggtcg gggtggaagg tcgccatgcc ccagtggtag
     3301 ccgtgcccgg gagcctcgta ggcgcgggcg agcggcgacc cgatcatcac ggcgtcggcg
     3361 ccgcaggcga acgccttgga gatgtcgccg ccgttgcgca tcccgccgtc ggcgatcacc
     3421 ttgacgtact cgccggtctc gagcatgtgc tgcgagcggg cggcggcgac gtcggcgatc
     3481 gcggtcgcct gcggcacgcc gatcccgagc atgccgcgcg tcgtgcatgc cgcgcccggg
     3541 ccgacgccga caaggacggc cgcggcgccg gttcgcatca ggtgcaggcc ggtcgagtag
     3601 gaggcgcagc cgccgacgac gaccgggaca tccggcggca cttcctcgcg gatgaactct
     3661 ttgaggttga gcggcgggcg cgactcgtcg gtcgatacgt gctcggccga gatcaccgtc
     3721 ccctggatga ccagcacgtc gatgcccgcc tcgatcgtcg tctcgtagta gtcgcgcacg
     3781 cgctgcggcg tcagcgaggc ggcgcagacg acgcccatgt ccttgatctc gcggatccgc
     3841 ttcgcgatca gctcctgctt gacgggctcg cggtagatcc gctgcatctc ggcggtcgcc
     3901 tcgtccttgt cgaagccgct gatccgctcg agctgctcct cggcatcctc gtagcgacag
     3961 aagacgccct cgaggttgag caccgcgagg ccaccgaggc ggccgatctc gccggcggtg
     4021 cgcggcgaca cgacgccatc catcgcggac gcgagcagcg gcagctcgaa gcggtagttg
     4081 ccgagcgtcc aggtgatgtc gatgtcgtcg ggatcgcgcg tgcggcgcga ggggacgatg
     4141 gcaatgtcat cgaagccata ggccctgcgg cccttcttgc cccgaccgat ctcgatctcc
     4201 atggcgcgga tcctaggttc gacggcggac gagtcccgag gttaccaacg tgcgttttcc
     4261 gcatcgccat gcggaattcc cgcaccttgc gggaattggc cggggctcac gcccccgtac
     4321 ccccggtggt gcctggccgg ccggagctac ccgggctgca gcgtcggcgc ctcgagcgtc
     4381 tcgagctcga ccgccgtgga gagccggtcg acctcgcgcg ggtcgaggac gccggcgccg
     4441 aagtgctggg tcgactcggc gccgcaggcg acgccgaagc gcaggcactc gtcgtcgggc
     4501 aggccgtcgt agcggccggc gacgtagccg gcgaggaagg catcgccaga gccgaccgac
     4561 gacacgggct cgagcggctc gagcgtcgcg cgggtcagcc gtcgcgggcg cgtggcgtcc
     4621 gactcgtcgc cgtgcagcgc gaggcagccg tcggcgagcg tcatgatcgc ctcgcgcgcg
     4681 ccgaggtcga ccatctcggg cagcgccgtg cgacggtcct cgtcgtcggc gaactcgtgg
     4741 ccgacgagct cctccgcctc gttctcgttc ggcgcgatca cgctcggctg ggcacgcacc
     4801 gcgagccgca gcggctcgcc ctcgcagtcg agcaccgtcg tcacgccgac gcggcgcagc
     4861 tcctcgacca gccgtgcgta gaggccggcg tccaccccgc gcggcaggct gccgctgaag
     4921 acgcagagcg cggcaccctt ggcgaggtag agcagcttgt cgacgaacaa cgccagctcg
     4981 gcctcggaca ccgacgggcc gcgctcgttg atctcggtct gctcgcctga ggtcgggtcg
     5041 acgaccgcgg tcgaggtgcg cgactcctcg cggatccgca cgaagtcgct gaggatcgcc
     5101 tcgtcggtca gctgctcgat gatccgcgag ccggtgtggc cgccggcgaa cccggtggcg
     5161 atcaccggcc ggccgagcga cttcagcgct cgcgcgacgt tgacgccctt gccgccgggc
     5221 atcgaggcct gctcgactgc gcggtggcgg tggccgagcc ggaagctggg gaccgcgagc
     5281 gtcttgtcga tcgcggcgtt gagggtgacg gtgatgatca tggcttcccg gctgggtcct
     5341 gttgatcccg cgctctgact cgctcccgct ctcggcgcct ggtcgctgca cggcgatgtc
     5401 ggtgccgccg ccgcatctgc attcccaccg cgccggtgac cgaaacgatg accaagagag
     5461 cgagcgccgt caagggcggg cccagactcg cgacgactcg ccgcagcgga ccggcgcccg
     5521 gcacccgctt cgccgtcagg agcgcgaccc gctcgacgac ctcaccatcg cggcggacga
     5581 cgacggtccc gacgggcgtg cctgcggcga tctcccccgc gagctcctcg ggcgccctga
     5641 cccgctgctc gagccgttgg tcgcgccgcg cggtcaccgc cagcgcacgt cgcgggacca
     5701 gggcgaccgt ctcgtcgtgg tgggcgacat ccgcgcgcgc cactgcgcgg cctttacgca
     5761 acagcggcac gcggcggtac tgggcgagcc cgtagcgcag cagcgcgagc gtgtccgcgt
     5821 cgcgtgccgc ctcgctcggc tcgccgagca cgacgctcac gacctgcgcg ccgttggacc
     5881 cggttgccga gccgaccagc acgtaaccgg cctcgctcgt gtagccggtc ttgatgccgt
     5941 cgacggccgg gacactggcg atcaggtcgt tgcggttgcc gacgacgcgc ggctcggcgc
     6001 ccgactccag cgtcgccgac ggcgagtcga cgatccgggc gaagcgccgg tcgcgcagca
     6061 gccggcgcgc caacgtcgcg aggtcacgtg cgctcgagta gttctgcggg tcgtcgagcc
     6121 cgatcggatt ggcgtaactg gtgtcctcga ggccgagctc gccggcgcgc gcgttcatct
     6181 cggcgacgaa tgcctcgcgc gaaccggaga ccccctcggc gagcgtcgcc gccgcgtcgt
     6241 tggcgctctc gagcatcagc gcctcgagca ggtcgctgac ccgcatccgc tcgccgggcc
     6301 gcaggtcgat cttcgactcg gccggtgccg cctggtagtc ggcggcggca tagaccgcgt
     6361 cgagcggagc gcgctcgagc gtcagcaggg cagtcatcag cttcgtcgtg cttgcgatcg
     6421 agcgacgctg cgtcggttcc ttctcgagga tcggcgtccc gtcgcccgcg tcgacgagga
     6481 tcgccgcgtc ggcgccctcg aggctcggac gctgcggcgc ctggccgctt gccgccggta
     6541 cgaccgacca cgccagcgcg accgccgccg cgacaccggc cagccgccgc acgatcactc
     6601 gcgcagcttg atcttctgac cggcgatcaa ggcctgcgga tcgagctcgg ggttgagctc
     6661 ctggagcgtc tcgacaggca cgccggtctg ctcggcgatc ccaccgagcg tgtcgcccgc
     6721 cttgatcgtg tagttcgccc gttgccgcgg tcgtggcctc tctgcggttc cggcggtctg
     6781 ctggctcgcg ggctgcgaga ccgacggatc gccgcccgag gagcctccgc cgctgagcga
     6841 gctgccgatc acgaccagga ccgcgagcgc acagacggcg atcgcggctg gggcgaggat
     6901 gcgggcggag ctgcgggagg agtgggtcgg catggcctgg ctcaggcggg cagcataggc
     6961 gctcaggcgc gccaggccag tgcgcgcaac tcctcggccg aggctgccgc ggcagaggcc
     7021 ggatcgccgc cgcgatccgc gtgggcgttg acgatcgaac gcgaggcggc gaccaagccc
     7081 gccgccgggt gcggcgcgaa ggcgggtccg agcgcgtcga cactcccgcc ctgagccccg
     7141 acgccgggca gcaggaagat cgcgtgcggc atcagctcgc gcaggcggcc gagccgctcg
     7201 ggccgggtgg cgccgcagac cgccccgacg agcgacagcc cgcagtcgcc gagctggtcg
     7261 gcgccgaaga cgtcggccag ctgggcgagc cgctcgtgca gcggccgtcc gtccgcgtcg
     7321 aggtcctgca aatcggcggc gcccggattg gaggtgttga cgagcaggaa gcagccagcg
     7381 tccgccgcgg ccgcggcctc gaccaacggc gcgatcgcgt cgccgccgag cagcgggttg
     7441 gccgtgaacg cgtctgcgcg caggccctcg acggtcccga acggcccatc ggtcgacccg
     7501 accagcgcct gcgcataggc gcgcgctgtc accggcacgt cgccgcgctt gccgtcggcg
     7561 aggaacagca ggccgtgcgc gcgagcgacg tcgcgcgtcg cctcgagcgc cgcccagccg
     7621 ggagcgccaa ggcgctcgaa gcaggcgagc tgcggcttga ccgcgacgca tgccgg
//
LOCUS       DATABV010000209        26834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_470519, whole genome shotgun sequence.
ACCESSION   DATABV010000209 DATABV010000000
VERSION     DATABV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 26834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26834
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470519"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /gene="mltG"
                     /locus_tag="VHF88_09550"
     CDS             <1..1161
                     /gene="mltG"
                     /locus_tag="VHF88_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX2162049.1"
                     /translation="DAERRAAAREFLHGRGGAARTARPAGAAPRRATAGDQRRTGGRR
                     LAIASVVGGLAVLVVAWFLLSLFQPLKGDGSGEVTVTIPSGASVGQIADILEQEGVVA
                     SAFFFRTRASLAGKGGDFKAGEFPLREDMSYGAAIEALSESPPPPDTVTITVPEGLSR
                     SETAALIGDSLRGDYMKATIRSPRLDPAEYGGRGAKDLEGFLFPATYELKPGQSVRAL
                     VDRQLDAFERELAKVDLRYAKSKNLTAYDVLTIASMIDREAQLPRERRLVASVIYNRL
                     SQGIHLGIDATIRFATGNWSEPLKESELAIDSGYNTRLNPGLPPGPIGNPGLAAIEAA
                     ARPAQTDFIYYVVKPCGNGAHAFSSSDAEFQEDVARYNAERERQGGQSPTEC"
     gene            1161..1958
                     /gene="aroE"
                     /locus_tag="VHF88_09555"
     CDS             1161..1958
                     /gene="aroE"
                     /locus_tag="VHF88_09555"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX2162050.1"
                     /translation="MGKTLLGVAGWPVAHSRSPAMFAPALAELGLDWRYVHLPLPPER
                     FAETARSLEASGYRGINVTMPHKRAAHDLADELTPAAAAIGAANTLTFDDGRIEADNT
                     DAGGFLDALDADPTDWRCLVLGAGGSARAVVWALREAGAAEVSVWNRTAERAAALADE
                     FGVRQVTRPEAADLVVNCTSVGIDRRQKQSEALAAFPLDWIDSPQTFFDLTYGSGTTA
                     LGGWAAARGARVIDGLEMLVRQGARSLERWTGREAPLEAMRSGALGS"
     gene            2049..2621
                     /locus_tag="VHF88_09560"
     CDS             2049..2621
                     /locus_tag="VHF88_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162051.1"
                     /translation="MAVSRPLLIALVAAVLGLAAFYATQGSRDSTETAAPVAPVVPAE
                     TANSSRTKDAQADEASSQPTRRKPEQAQARRDSAAERRLARAKRIGMPLPVARALRAR
                     RTVVLYFRGSPSADDRATARAVAGLRGQRGVTVFADRIGRLGRYRAVVGDLGIAQAPA
                     VVIIGRGRAARVVEGYIDPTTLAQDVADAR"
     gene            2618..4429
                     /locus_tag="VHF88_09565"
     CDS             2618..4429
                     /locus_tag="VHF88_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX2162052.1"
                     /translation="MTGGFGGDGGLGSGGGPPHLRPVPSPGSGGPAAAHDREPDPGTG
                     LTPPRQASARGRFISDVVVELGFAARERVDAAVDEARASGRTAEQVLVESGALTPEQL
                     ARATAERFGLHHVDLNVYKPDLGAANLIPPQAARRFSAVPIGFDEGNLLVAMVDPSNV
                     LALDDMKLLTGHELRPVVASPDDIAALISRMSRLDDAVAEAVQEEDDEAEAAVTDIRE
                     SAEDAPVIKLVNSIIAQGVEEGASDIHFEPDGRDMRVRFRIDGVLSETTSIPRRMVAG
                     VVSRVKIMADLDISERRVPQDGRVGLKVENHAVDIRVVTMPSVHGEGIVMRLLDKDQA
                     LLTLDKLGMSGEPRERFELAFRQAYGAVLVTGPTGSGKSTTLYAALNAINSVDKNIIT
                     IEDPVEYQLAGVNQLQVNHKAGLTFARGLRSMLRADPDVIMVGEIRDSETARIAVESA
                     LTGHLVLSTLHTNDAASAITRLTEMGIEPFLTSSAVDCVVAQRLTRRLCPSCKRRTIL
                     DVDALRESGFQAAFDVEAYEPVGCSRCNGTGYKGRVGLYEVMVLSDEIRELAVKRASA
                     DEIVKVAVHQGMRPLRDDGLDKVRQGLTSIAEVTRVS"
     gene            4469..5542
                     /locus_tag="VHF88_09570"
     CDS             4469..5542
                     /locus_tag="VHF88_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX2162053.1"
                     /translation="MEFDFADVLIEVMERNASDLHLTAGSPPMVRLRGKLTALDYPPL
                     DPTTVREVVYSILTNDQRQRLETEWQIDLAYSIPGKARFRVNAYFQRASLGAALRLIP
                     QEMPRLADLGLPATLREFTKKPRGFVLVTGPTGSGKSTTLASMLDLINDERDEHIMTI
                     EDPIEFLHRHKRCIVNQREIGADAQTFSLALKAALRQDPDVILVGEMRDLDTISTALT
                     AAETGHLVFATLHTQDTAQTIDRIVDVFPPQQQHQVRMQLSVALQGIVTQQLLPTADG
                     MGRVAATEVLVPTNAVRNLIREGKTHQIHSALQTGVAHGMQTMDASLADLVRKGKITR
                     ELAESRSTSPEELRRLMGTVQAA"
     gene            5586..6800
                     /locus_tag="VHF88_09575"
     CDS             5586..6800
                     /locus_tag="VHF88_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2162054.1"
                     /translation="MGTFAFKALDVAGIPTRGEIDADDKQVVVSQLRSRGLTVLDIEE
                     QKPTDVGDIFARFKKVKSEDLTIATRQLSTMVSSGMTLLRSFHVLEEQTENDKLRDTF
                     TAVRKDIEAGLSLSQAIGRHPDVFNDLYVAMVSAGESGGILESTLLRVADQLEKDDAL
                     RRQIKAAMIYPGLIASFAGLVLIALVSFIVPVFEQIFKDFGGELPAITQFTVWLSHMF
                     TQRWYVLILVAVAVVWSFRKWKGTERGRVQWDTLKLKTPMKIGDIVQKVALARFSRTF
                     SGLVAAGVPMLEAIDITGRTSGNKVVEMAMEEVERSVKKGGTIAAPMRAAPEAFPVMV
                     VQMIGVGEETGALDQMLSKIADFYEDQVAASLKALTSILEPIMIMLVGAIVGFIVISM
                     YLPMFRVYDQIQ"
     gene            6852..7049
                     /locus_tag="VHF88_09580"
     CDS             6852..7049
                     /locus_tag="VHF88_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162055.1"
                     /translation="MRATTIRFTPELWALLQREAEREEVSVAQYVRDAALFRVAYGAG
                     VRREHEAPGEGADAPPALRPR"
     gene            7095..8915
                     /locus_tag="VHF88_09585"
     CDS             7095..8915
                     /locus_tag="VHF88_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2162056.1"
                     /translation="MALSLDARLVARARRGSDAAFERLYARHQPAILSFCRHLTGNRE
                     DAEDAVQHTFLAAYRRITGPGDDQLELRPWLFVVARNRCLSLLRARRELAVPAVDADS
                     RVFDGLSAEVERREDLRHLVRDMARLPEPQRAALVLSQLDALDYREIGTVLDVGPEKV
                     KALVFQARSSLASARDAREAPCLDIRREIATARGSALRRRLLRRHVGQCDGCSEFEQT
                     VLRQRRELASLLPVVPSAGLGQAVLDSASGREAATAAGAGGGGATTVGVGGGAIGGAG
                     AGGGGLAGTFGLVGAGGAAKIAVVAVAVGGGGAGAVAADLPSRIDAAVLSIGSSGHAS
                     GDRGAGSAVPGGTDDGEQTTPSAGEGDGSDRDSTGDRKHGLDRRDSAMRGTVNGGRSD
                     GDAPRVGVRDSYASGGVAGDAGDGGDDPSGSRPGSAPGDELPPGLAKRDELPPGLIRR
                     DGRPPGLAKRDGASPGRSGGAPGAGRGAPPGDGNAPGRGNPAGQRDTRGQDNGGGQGN
                     GNGGGQDNAGRGNGSGGSQGTGEGGAGQGNGGDSPGNSGGNGSGPGNSDGGPAPPEPG
                     QGGPPGGQGDPGGGRGTRGPIGQGDGGGSPGRSDGAGNPR"
     gene            complement(8970..9278)
                     /locus_tag="VHF88_09590"
     CDS             complement(8970..9278)
                     /locus_tag="VHF88_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2162057.1"
                     /translation="MNDEGIIGSTTKHGYSGDRQKIVNRLRRIEGQVRGVERMVDEDR
                     YCIDVVTQISAIQAALDKVSLGLLEDHARHCVVGGHGDGSPDELTDELMAAVARLVRR
                     "
     gene            9362..9577
                     /locus_tag="VHF88_09595"
     CDS             9362..9577
                     /locus_tag="VHF88_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEX2162058.1"
                     /translation="MTPATAELEYSVPGVSCGHCHTAIADEVERVAGVRRVEVDLDAK
                     RVVVRGGALDDAAIRAAIEAAGYEVAP"
     gene            9574..10422
                     /locus_tag="VHF88_09600"
     CDS             9574..10422
                     /locus_tag="VHF88_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162059.1"
                     /translation="MRSAMRIGVFAGGLAVVFGAAFLAGGVFDPDVEDGEPTAHRAAA
                     ATHEGDASAPARVLLASSRFERGAGQTLAFRVVDREGRNVEDFDVEHGRRMHLIAVRH
                     DLTGYRHLHPRPTGDGGWTVDVDFMAAGPHRVYADFVTRGKPYTLSADVDVAGRYAAQ
                     TLPAPATSSDAGDGYEVEVARHGGERRFTVSRDGEPVDDIEPYLGARGHLVAIAEGDL
                     AFEHVHPKDGATEGREIRFDVAFGGPGRYRLFLQFKHDGKVRTAAFTERVGGGEPAHG
                     DDGHGY"
     gene            10412..12661
                     /locus_tag="VHF88_09605"
     CDS             10412..12661
                     /locus_tag="VHF88_09605"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2162060.1"
                     /translation="MATERLDLPIAGMTCASCATRIEKRLNRLDGVDAAVNYATEKAT
                     VAYDPRTVETDDLVAAVEAAGYQAVVPVVDAGGGEEAPPAAEDSTVQLRRRLAVSAAL
                     SLPVLVISMVPALQFDYWQWLVLQLATPVVLWGAWPFHLAAWRNLRHGAATMDTLISL
                     GTLAAWLWSLYALFLGEAGMTGMTMTIDFIPERGAGADHIYLEVAAVVTTFILAGRYF
                     EARAKRRAGAALTALLELGAKDVGVLGEDGSEQRIAIDQLKAGDRFVTRPGEKVATDG
                     VVEQGSSALDMSLLTGESVPVEVGPGSEVAGATVNVGGRLIVRATKVGSNTALAQIAR
                     LVTDAQAGKAPVQRLADRVSAVFVPIVVALAVATLGFWLGAGEGAAFAFTAAVAVLII
                     ACPCALGLATPTALMVGTGRGAQLGLLIKGPEVLESTRRVDTVVLDKTGTVTSGRMQL
                     VDVTVDGTERREALRIVGSVEDASEHPVARAIAAAARSELGELAPVASFANRDGLGVD
                     GVVDSDRAIVVGRPSLLEQRGLELPDGLEQARRTAEADGRTAVLAGWDGRVRAVLAVA
                     DTPKPTSAAAVQRLKRLGLRPLLLTGDNRATAEAVARAVGIDEAIAEVLPAEKAGVVR
                     RLQDEGRVVAMVGDGVNDAPALAQADLGIAIGTGTDVAIEASDLTLVSGDLLAAPDAI
                     RLSRATLRTIKQNLGWAFGYNVAALPLAAAGLLNPLLAGAAMALSSVSVVANALRLRR
                     FRAGQGSKR"
     gene            12658..13230
                     /locus_tag="VHF88_09610"
     CDS             12658..13230
                     /locus_tag="VHF88_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX2162061.1"
                     /translation="MNGHSHHAAVASSGRALDPVALSATLHCLTGCAIGEVVGMVVGT
                     ALGFPELGTVALAIALAFLFGYSLTSLPLLRAGMAFRAVVPIALSVDTVSIALMETVD
                     NAIMLAIPGAMKSGAGDILFWGALSVALVVAGIVAFPVNRWLIQRGKGHAVLHETGIH
                     GGPPTKLVAAIAVLAAVFGTTVLVAEALGG"
     gene            13233..13847
                     /locus_tag="VHF88_09615"
     CDS             13233..13847
                     /locus_tag="VHF88_09615"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX2162062.1"
                     /translation="MAALALGAYALYMALAFGLRTAVQLRRTGASGFEGVSGRPGSAE
                     WWAGVLFAVAIAVGVTAPLLALLGIVEPVRAFDVTAVHAVGVVLFGAGLAGTLAAQVA
                     MGDSWRIGVDDSTVTELVVDGPFAVVRNPIFAAMLPACTGLALMVPSVVSFVGLAALV
                     IALQLQVRVVEEPYLLRAHGSRYREYAARVGRFLPGIGRLGVDR"
     gene            complement(13999..15141)
                     /locus_tag="VHF88_09620"
     CDS             complement(13999..15141)
                     /locus_tag="VHF88_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2162063.1"
                     /translation="MLLLAAKIGDEVFRRIGQPTIVGEILAGVIVGPSLLGFVEPDAV
                     LEVFAELGVVFLLFWVGLETKVSELREVGKTALLVGILGVVLPLAGGVGLGLGRGDDA
                     ATAVFLGAALVATSVGITSAVLIQLGVLRTRVARTILGAAIVDDVLALIILSVAVGLA
                     AEGGVDVTGILWVLAISLGFVGFFALGGTRVTQRWPQVFKAPRFSESPLLPAVILCLG
                     LAAFAAQIGLAAIIGAFLAGLMVAETKEQHPVEDEVAPLYAFFPPFFFAFIGTELDLG
                     ALADPGALGLLALVTVLAIATKFGGAWLGARSLGRSEAAAVGFGMVPRGEVGIIVAGI
                     GRTTGVIDDELFAVIVGMSILTTLLVPPVLRRLLAPAAAETASEPV"
     gene            complement(15201..15647)
                     /locus_tag="VHF88_09625"
     CDS             complement(15201..15647)
                     /locus_tag="VHF88_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162064.1"
                     /translation="MELRRALLLFVVVLGVAAIASSIVRPPDGRDERSATDRSGGTGT
                     RPGSAAAEPTPESPPPTTIEFEPAAKPQTHELTVGQPATVLVNVQTPAEVRIPSLGLT
                     GVAEPLTPAVFEVRATEPGRHAIAIQPAAADTLPSKAGVLEVVAPS"
     gene            complement(15565..16863)
                     /locus_tag="VHF88_09630"
     CDS             complement(15565..16863)
                     /locus_tag="VHF88_09630"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX2162065.1"
                     /translation="MRRALAALTVLVLIALTARAAASMEAGLDYLNDAGDSIDALARG
                     DLQGFVANQPLMGSFSMLLRAPFVAAVFHQSVEAVYFAGAVPCLAATLVLGLTLARLL
                     AEREQPATVQGLAAGLAVINPLTFQALHWGHPEELLAGALCVGAVLAAMRERALTAAV
                     LLGLALATKQWALLAVLPVLLAAPSRRLTVALVAGAIAAALTLPLMLGDPGQFGSVAR
                     AAAGQDAIGTSTTPWNVWWPFADKVAVAPFGPRWMAPQWVATVSHPGIVAVALPLSLL
                     LWRRADRRPDDALLLLALLFLLRCLLDNWNNDYYHVPLVLSLLAWESVRRPGVPYLTM
                     AVLILVSASFWPIEDRMFGDSRDHAGVLFALYMAWALPLATGLALLLYRPATAASALA
                     GSVQLLRGEYHRRRGASARPAAVRGRPRRGGDRQLDRPSA"
     gene            17091..17534
                     /locus_tag="VHF88_09635"
     CDS             17091..17534
                     /locus_tag="VHF88_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2162066.1"
                     /translation="MLRKLRERIQKDESGFTLIELLVVILIIGILAAIALPAFLGQQT
                     KGQDSEAKSDARNAVSQLESCYTDAQTYVGCETDAAVGFDTLNVEGASIETGADTFTV
                     TAVSKSDNIFTISKAADGTTTRGCTVVGGDDGGCTESWTGSGSGS"
     gene            17739..19292
                     /locus_tag="VHF88_09640"
     CDS             17739..19292
                     /locus_tag="VHF88_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2162067.1"
                     /translation="MARARIGSQSGFTLVEVMVAIVLLLVGVLGAVSLIDRANAQTST
                     TKAREGATALARSLLEISRGVPYAELTSERVLAELDARGAGFADGDAAAGHQIASRGF
                     VYTVEPTVCSMDDPKDSLGPHDELGVTFCANSDVAAGGATTVDRNPDDYRRVVVRLSW
                     RVGAGGPVQSLTQTGIVTNPVGGLGPNVLALTPISPATPTITGDDQHANYDVLTSAAA
                     QDVAWSVNGRRMGSAEGAGTEWDFTWDLGPVDDPAVYDCTYVVQAEAFDDKARAGAPR
                     ALTVTVNRRRPFGPRDFAGGANGNGGGRGDVDLQWDANLECDVQRYEVYREVDGVPDD
                     GPVCEVDRGERTECVDEAAPSTGELTYRVFAFDLDRDGNERRGDPSPHAVVVPDPVIN
                     DAPEAPAGLTACTGGQFDGPCLDIEGNPASDGTAVLSWPPVANPDTDGDAIRFYRVYR
                     AGIGAAAPTYADRLDVLFPVFDEQGEPVDPLVFVDGTASGPHRYWVTAVDERFAESGP
                     VGPVEWGAP"
     gene            19289..20185
                     /locus_tag="VHF88_09645"
     CDS             19289..20185
                     /locus_tag="VHF88_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162068.1"
                     /translation="MTRIVREESGLMLVELLVVMVLSGIVLGATLTTFNQFERTTGTN
                     QRQTEAQEQVRVGLAAVARELRNLASPTNERPHAIRHAGASDLVFQSMSSTVTRRVRY
                     CLDAEQRTLWRQVQLEPFSDPSQTACPDVGWGSERTAIADVVNGARPVFGYNVEDPMG
                     ITEISATVWVDVNPGVAPAETSLQTSIFLRNQNRSPTAVSTAELSGSSIVLNGSESFD
                     PEGRSLRFYWYDRAVSGHEACGEDLPSDLPQDGCVATGIVATYTPPEPGSRSLYLVVA
                     DPAGLTDQAPDESVCVPGGPSC"
     gene            20179..21402
                     /locus_tag="VHF88_09650"
     CDS             20179..21402
                     /locus_tag="VHF88_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162069.1"
                     /translation="MLRLSERLRTEQDGNLLVIAMTMMTLMMVTGFATLSIVDTQTDV
                     AMKERQHESSFNLAEGVLNAQTYVLARRGTGDAGASQFPDACVNGTTHSLCPSEEQLA
                     RSYSAAVQVDYGDDTNWTTRVRDNPNGTFYDPTAVAAAARYDANGDRQLWVSAEATVR
                     GRTRQIVALIRVEDRPVTFPSYALLGGMFATSNSGRGTIVDATDPSSLGIAVRCVGEP
                     SKQSSCLKFDPKKDQLNPPKAYTTGVPDTAAISLDDLQALEEYARGSGTYYGSCPGPN
                     PNGAVVVIESGDCPFSNSSPAAPGASKCCNTPTRPGLLIVKCGTVTLKGNIEFYGIIY
                     MPQGDCSLTGYVVTTQGTSLISGGVIIDGMDRGLLAGSSGDNIVFNPLAFQNVRAAGT
                     AGVVQNTWREVPDDL"
     gene            21454..22092
                     /locus_tag="VHF88_09655"
     CDS             21454..22092
                     /locus_tag="VHF88_09655"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2162070.1"
                     /translation="MVGHAIAFKLRDERGLTLVELLVTILVMGLVVTGILGVLDTSAR
                     IAPKDVERANAIGEAQTGLNRIVRELRQAYRIVGWTPSSVQMRVNILRDDPSTAGPDY
                     ANVTVDYSCGGDPGRCVRREAPVGTTLPATGIVVVGRVLNGGAAPPEGRHVFDFDQSP
                     DRSVGVTAASVRPTYVSVRIEVPAAGERGSGGHQHSVVLDDGLYVRNVGGVH"
     gene            22094..23491
                     /locus_tag="VHF88_09660"
     CDS             22094..23491
                     /locus_tag="VHF88_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2162071.1"
                     /translation="MGRAPRNAGRRSPRSQDGFTLIEVMVALLLLTVGIFAVATTFDF
                     SRASTSQSELETAAVDRAQREIEAIRSLPYEQVAHPLGGIAPAAGDEADPAARVSGAS
                     FAWDRSNDAASEPLVISADGAVPMKQTIARGAGDRFGYTIWRFVTATDEPACVAAVEC
                     PDDGGGYRRVTVVVRVSGVGETLAPVWTSTTVIDPAAAANNDGTPQTLCQKPDGSGLE
                     LCSEEVDGTSMDLYLTNTPAGGHDARVEPAASGPLHKTVAIPPSCHAGAAVGCPVPDL
                     MVGEPLAQLAEEDPVPPLRTFAADVEPSVAAGRPLLEDVACDATPTRHGGDDVNLRGA
                     YWVSPVLDAPIHLRGVGGLYLYARTWSETAADVTLCGIVYRAPASVANPVLSPPHEIG
                     RIDVTTPWPETFQPVAATFDFDLDPGDPHATVEADERIGLRLWVADGSADEIVLLYDH
                     PLNQSRLTLVVEETG"
     gene            23488..24912
                     /locus_tag="VHF88_09665"
     CDS             23488..24912
                     /locus_tag="VHF88_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162072.1"
                     /translation="MTSRLRICRRLHTEQDGVAIVVAIGALGVLLILSTVAFSASSQL
                     SDTSKVDRSARSAFQAAEAGLDVATYRINNLLPLGGGAGCVAGVEDATADGSCVAGEQ
                     LADGASWSYEVTPDLDEENYDELLPGGRCAGFPIQFDADDLDLTLRSRCITATGTAHG
                     QTRRVQARLALFTGTSSETPGTTLFPVGGIIGLDGVTLSNAAQVSGSVASNGQVELSN
                     AARVTGAAILGPGAPAPDVGNAATIGQGTVYRSPAEGPWVLAPVDIGNTATVNRNGNG
                     IGWTVESGTATYSSAYRELEIKNAARLNFSGGDYNLCRLEAKNATRFTVTPGAKVRLF
                     IDSPTRPGSGCRPGTGTFVAKNAFDLEADEKSAQFQIYIYGGDVDGNPAFEVKNSSGF
                     HALLHAPLASVEFKNAASFWGAINAKRVEFGNASSFHWPAEGISGLTPDTDTPQNTLT
                     LFQRSAWRECRSAPSDPENPHSGC"
     gene            24971..25729
                     /locus_tag="VHF88_09670"
     CDS             24971..25729
                     /locus_tag="VHF88_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX2162073.1"
                     /translation="MPDPIAIAVLALLGLVVGSFLNVVVHRLPRGESLSHPRSRCVGC
                     GTQIAAYDNVPVLSWLMLRGRCRHCGEPISPRYPAIEALTGVAFAGVAAIAGVDAGLW
                     LALPFAALMIAVAAIDLEHRIVPNRLLAPAAVWAVVAWAAVDLDFLPEALAAGAGAFA
                     FLLLAALAYPAGMGMGDVKLAGVMGLYLGLSVVPALLAAFLAGSVVGIAIVLRDGRDA
                     RKTGVPFAPFLAFGGLIGLLAGSDAISLYSDHFL"
     gene            25785..26768
                     /gene="pilM"
                     /locus_tag="VHF88_09675"
     CDS             25785..26768
                     /gene="pilM"
                     /locus_tag="VHF88_09675"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX2162074.1"
                     /translation="MALSLRKNSDGGTVGLDIDGRFVAAARMHGGRLASVASAELPAG
                     ALADGEVADRAALAAALKEFAAANKLPRRVRLGVSNQQIVVRVLDLPRIDDPSERDAA
                     LRFQAAEAIAMPLDEAVLDHQIAGYTGGGDSGERMQVVVVAARRSMIDEYLGAASDAG
                     LKADGIDLDAFALVRMLATTDASPLSAVGDEDGAVQDGLAAGPQTARVFCHLGGVSNL
                     AIALGSSCFFTRALATHFDDEAAGSRLADEIRMSIDYYMAQPGALGVGDAVVSGEGAR
                     DEALVGELATQLGLPVAAAEPLGTLDATAVPAGEDRYRYTVAAGLALGVAA"
BASE COUNT         3945 a         9566 c         9603 g         3720 t
ORIGIN      
        1 gatgccgagc gtcgcgccgc cgcgcgcgag ttcctgcacg gtcgcggggg cgctgcgagg
       61 actgcccgac ccgcgggcgc cgcgccgcga cgtgctaccg ctggagacca gcgacgcacc
      121 ggcgggcgac gtctggcgat cgcctcggtg gtcggcgggc tcgcggtgct ggtcgtcgcc
      181 tggttcctac tctcgctctt ccagccgctg aagggtgacg gttccggcga ggtcacggtc
      241 acgatcccga gtggcgcgag cgtcggacag atcgccgaca tcctcgagca ggagggcgtg
      301 gtcgcgtccg cgttcttttt ccgcactcgc gcctcgctgg ccggcaaggg cggcgacttc
      361 aaggccggcg agttcccact gcgcgaggac atgagctacg gcgcggcgat cgaggcgctg
      421 agcgagagcc cgccgccgcc cgacaccgtg acgattacgg tcccggaggg cctttcgcgc
      481 tccgagacgg ccgcgctaat cggcgactcg ctgcgcggcg actacatgaa ggcgacgatc
      541 cgctcgccgc gactcgatcc agccgagtac ggcggtcgag gcgcgaagga cctcgagggc
      601 ttcctgttcc cggcgaccta tgagctgaag ccgggccagt ccgtgcgggc gctcgtcgac
      661 cgccagctcg atgccttcga gcgcgagctc gcgaaggtcg acctgcgcta cgcgaagagc
      721 aagaacctga ccgcctacga cgtcctgacg atcgcctcga tgatcgatcg cgaggcccag
      781 ctgccacgcg agcggcggct cgtcgcctcg gtgatctaca accggctcag ccaggggatc
      841 cacctcggga tcgacgcgac gatccgcttc gcgaccggga actggagcga gccgctgaag
      901 gagtccgagc tggcgatcga ctccggctac aacacgcgcc tgaatcccgg cctgccgccg
      961 gggccgatcg gcaaccccgg gctggcggcg atcgaggcgg cggcgcggcc agcgcagacc
     1021 gacttcatct actacgtcgt caagccgtgc ggcaacggcg cgcacgcgtt ctcgtccagc
     1081 gacgccgagt tccaggagga cgtcgcccgc tacaacgccg agcgtgagcg ccagggcggc
     1141 cagtcgccga ccgagtgctg atgggcaaga cgctgctcgg cgtcgccggc tggccggtcg
     1201 cgcacagccg ctcgcccgcg atgtttgcgc cggcgctcgc cgaactgggg ctcgactggc
     1261 gctacgtcca cctgccgctc ccgccggagc gcttcgcgga gaccgcgcgc tcgctcgagg
     1321 cgtccggcta ccgcgggatc aacgtgacga tgccgcataa gcgtgcggcg cacgacctcg
     1381 ccgacgagct gacgccggcg gcggccgcga tcggggcggc gaacaccctg accttcgacg
     1441 acgggcggat cgaggccgac aacaccgacg ccggtggctt cctcgatgcg ctcgacgccg
     1501 atccaacgga ctggcgctgc ctagtgctgg gggcaggcgg atcggcacgg gcggtcgtct
     1561 gggcgctgcg cgaggccgga gcggccgagg tgtcggtctg gaaccggacg gccgagcggg
     1621 ccgcggcctt ggccgacgag ttcggcgtac ggcaggtgac gaggccagag gcggcagacc
     1681 tggtcgtcaa ctgcacctcc gtcggtatcg atcgccgcca gaagcagtcc gaggcgcttg
     1741 ccgccttccc actcgactgg atcgattcgc ctcagacgtt cttcgacctc acgtacggat
     1801 caggcaccac cgcgctcggg ggttgggccg cggcgcgggg tgctcgtgtg atcgacgggc
     1861 tcgagatgct cgtccgtcag ggcgcgcgca gccttgagcg ctggaccggc cgcgaggcgc
     1921 cgctggaggc gatgcggtcg ggagcgctcg gcagctaggc ggtcgccttc gcacggccgc
     1981 gacgcgacgg ctctgcacaa cttcgcccgc cataaggctt cgctgtcgat ctgccgatag
     2041 ggcgggagat ggctgtctcg cgtcccctcc tgatcgccct ggtggcggcc gtgctcggcc
     2101 tcgccgcgtt ctacgcgacg cagggctcgc gcgactccac ggagaccgcc gcgcccgtcg
     2161 cgccggtcgt tcccgcggag acggccaaca gctcgcggac aaaggacgcg caggccgacg
     2221 aggcctcctc gcagccgaca cggcgcaagc ccgagcaggc gcaggcccgc cgcgacagcg
     2281 ccgcggagcg tcgccttgcg cgtgccaagc gaatcgggat gccgctcccc gtggctcgcg
     2341 ccctgcgcgc ccgcaggacg gtcgtcctct acttccgtgg cagcccctcc gccgacgatc
     2401 gcgctaccgc gcgagcggtc gccggcctgc gcggccagcg cggcgtgacg gtgttcgccg
     2461 atcggatcgg ccggctcggg cgctaccgcg cggtcgtcgg cgacctcggc atcgcccagg
     2521 cgcctgcagt ggtgatcatc ggccgcggac gcgcggcgcg cgtcgtcgag ggctacatcg
     2581 acccgaccac gctcgcgcag gacgtcgccg acgcgcgatg accggcggct tcggcggtga
     2641 cggcgggctt ggcagcggtg gcgggccgcc gcatctgcgg cccgtgccct cgccgggctc
     2701 gggcggtccc gcggccgcgc acgatcggga gcccgacccg ggcacggggc tgacgccgcc
     2761 gcgccaagcg agcgcccgcg gccgcttcat ctcagacgtt gtcgttgagc tcggcttcgc
     2821 cgcccgtgag cgcgtcgacg ccgcggtcga cgaggcccgg gcgtccggcc gtaccgccga
     2881 gcaggtgctg gtcgagagcg gcgccctgac gcccgagcag ctcgcgcgcg cgaccgcgga
     2941 gcgcttcggc ctccaccacg tcgacctcaa cgtctacaag ccggacctcg gcgcggccaa
     3001 cctgatcccg ccgcaggcgg cgcgccgctt cagcgccgta ccgatcggct tcgatgaggg
     3061 caacctgctg gtcgcgatgg tcgacccgtc gaacgtcctc gcgctcgacg acatgaagtt
     3121 gctgaccggc cacgagctgc ggccggtcgt cgcctccccc gacgacatcg ccgcgctgat
     3181 cagccgcatg tcgcgcctcg atgacgcagt cgcggaggcg gtccaggagg aggacgacga
     3241 ggccgaggcg gcggtcaccg acatccgcga gtcggccgag gacgcgccgg tcatcaagct
     3301 ggtgaactcg atcatcgccc agggggtcga ggagggtgcc tcggacatcc acttcgagcc
     3361 cgatgggcgc gacatgcgcg tgcgcttccg catcgatggc gtgctcagcg agacgacctc
     3421 gatcccgcgc cggatggtcg cgggtgtggt ctcgcgcgtg aagatcatgg ccgacctcga
     3481 catctccgag cgacgcgtgc cgcaggacgg ccgcgtcggt ctgaaggtcg agaaccacgc
     3541 ggtcgacatc cgcgtcgtga cgatgccttc ggttcacggc gagggcatcg tcatgcgcct
     3601 gctcgacaag gaccaggcgc tgctgacgct cgacaagctc ggcatgtccg gggagccgcg
     3661 cgagcgcttc gagctcgcct tccgccaggc ctacggagcc gtgctggtga ccggaccgac
     3721 cggctcgggc aagtcgacga cgctctacgc ggcgctcaac gcgatcaact cggtcgacaa
     3781 gaacatcatc acgatcgagg accccgtcga gtaccagctt gccggcgtca accagctgca
     3841 ggtcaaccac aaggccggcc tgaccttcgc gcgcggcctg cgctcgatgc tgcgcgcgga
     3901 ccccgacgtg atcatggtcg gcgagatccg cgactccgag actgcgcgga tcgccgtcga
     3961 gtcggcgctg accggccacc tcgtgctctc gacgctgcac accaacgatg ccgcgtcggc
     4021 gatcacccgc ctcaccgaga tggggatcga gccgttcctg acctcgtcgg cggtcgactg
     4081 cgtcgtcgcc cagcggctga cgcgacggct gtgcccgagc tgcaagcggc gcacgattct
     4141 cgacgtcgac gcgctgcgcg agtccggatt ccaagccgcc ttcgacgtcg aggcctacga
     4201 gccggtcggc tgctcgcgct gcaacggcac cggctacaag ggacgcgtcg gcctctacga
     4261 ggtgatggtg ctcagcgacg agatccgcga gctcgcggtg aagcgcgcct ccgccgacga
     4321 gatcgtcaag gtcgccgtcc accaggggat gcgcccgctg cgcgacgacg gactcgacaa
     4381 ggtccgccag ggcctgacct cgatcgccga ggtgacacgt gtctcctgag attcaggacc
     4441 gcggacgcgg ctgccgatag gggcgagcat ggagtttgac ttcgcagacg tcctgatcga
     4501 ggtaatggag cgcaacgcct ccgaccttca tttgaccgct ggctcgccgc caatggtgcg
     4561 tctgcgcggc aagctgacgg cgctcgacta cccgccgctc gacccgacga cggtacgcga
     4621 ggtcgtctac tcgatcctga ccaacgatca gcgccagagg ctcgagaccg agtggcagat
     4681 cgacctcgcc tactcgatcc ccggcaaggc gcgcttccgc gtcaatgcct acttccagcg
     4741 tgcgtcgctc ggcgccgcgc tgcgcctgat cccgcaggag atgccgcgcc tggcggatct
     4801 cggcctaccg gccacgctgc gtgagttcac caagaagccg cgcggcttcg tgctcgtgac
     4861 tggcccgacc ggctccggca agtcgacgac gctcgcatcg atgctcgacc tgatcaacga
     4921 cgagcgcgac gagcacatca tgaccatcga ggacccgatc gagttcctcc accgccacaa
     4981 gcgctgcatc gtcaaccagc gcgagatcgg tgcggacgcg cagaccttct cgctggcgct
     5041 gaaggcggcg ctgcgccagg accccgacgt gatcctggtc ggcgagatgc gcgacctcga
     5101 cacgatctcg accgcgctga cggcggccga gaccggtcac cttgtcttcg cgacgctgca
     5161 tacccaggac accgcgcaga cgatcgaccg catcgtcgac gtcttcccgc cgcagcagca
     5221 gcaccaggtg cggatgcagc tctcggtcgc gctgcaggga atcgtgaccc agcagctgct
     5281 cccgaccgcc gacggcatgg ggcgcgtcgc cgctaccgag gtcctggtgc cgaccaacgc
     5341 cgtacgcaac ctgatccgcg agggcaagac gcaccagatc cactcagcgc tgcagaccgg
     5401 cgtcgcgcac ggcatgcaga cgatggacgc ttcgctggcg gacctggtcc gcaagggcaa
     5461 gatcacgcgc gagctggcgg agtcgcgctc gacgtcgccg gaggagctgc ggcggctgat
     5521 gggaaccgtc caggcggcat gatgagaatc cgatcacacc cggcagagcc gggcgttccc
     5581 ggctgatggg aaccttcgcc ttcaaggccc tcgacgtcgc cggcatcccg acgcgcggcg
     5641 agatcgacgc cgacgacaag caggtcgtcg tatcgcagct gcgctcgcgc gggctgacgg
     5701 tgctcgacat cgaggagcag aagccgaccg acgtcggcga catcttcgcg cgcttcaaga
     5761 aagtcaagtc cgaggatctg acgatcgcga cgcgccagct ctcgacgatg gtctcgtcgg
     5821 gcatgacgct gctgcgctcc ttccatgtcc tggaggagca gaccgagaac gacaagctgc
     5881 gcgacacgtt caccgcggtc cgaaaggaca tcgaggcagg tctctcgctg tcgcaggcga
     5941 ttggacgcca ccccgacgtc ttcaacgatc tctacgtggc gatggtgagc gcgggcgaga
     6001 gcggcggcat cctcgagtcg acgctgctgc gcgtcgccga ccagctcgag aaggacgacg
     6061 cgctgcggcg acagatcaag gcggcgatga tctaccccgg gctgatcgcg agcttcgcgg
     6121 gcctcgtgct gatcgcgctc gtcagcttca tcgtgccggt gttcgagcag atcttcaagg
     6181 acttcggcgg ggagctgccg gcgatcacgc agttcaccgt ctggctgtcg cacatgttca
     6241 cccagcgctg gtacgtgctg attctggtcg ccgtcgccgt cgtctggtcg ttccggaagt
     6301 ggaagggcac cgagcgcggc cgggtgcaat gggacacgct gaagctgaag acgccgatga
     6361 agatcggcga catcgtccag aaggtcgcct tggcgcgctt ctcgcgcacc ttctccggcc
     6421 tcgtcgcggc cggcgtgccg atgctcgagg cgattgacat caccggccgt acctccggca
     6481 acaaggtcgt cgagatggcg atggaggagg tcgagcggtc ggtcaagaag ggcggcacga
     6541 tcgccgcgcc gatgcgcgcg gcgcccgagg cgttcccggt gatggtcgtc cagatgatcg
     6601 gcgtcggcga ggagaccggc gcgctcgatc agatgctgtc gaagatcgcc gacttctacg
     6661 aggaccaggt ggcggcctcg ttgaaggcgc tcacctcgat cctcgagccg atcatgatca
     6721 tgctggtcgg ggcgatagtc ggcttcatcg tgatctcgat gtacctgccg atgttccggg
     6781 tctacgacca gattcagtag ccggctcggc caccgcccaa ccctcgggga gctggcgcgt
     6841 tgtaggggta gatgcgcgcg acgacgatca ggttcacgcc cgagctgtgg gcgctgctgc
     6901 agcgcgaggc cgagcgcgag gaggtgagcg tcgcccagta cgtacgtgac gccgccctgt
     6961 tccgcgtggc ctacggtgcc ggcgtccggc gcgagcacga ggcgcccggc gagggcgccg
     7021 acgcgccgcc ggccctacgg ccgcgatagc ggctgcggcg gcgctcccgc cctcggcggg
     7081 tggataatgg gccgatggcc ctgtctctcg atgcccgcct cgtcgcacgc gcaaggcgcg
     7141 gcagcgacgc ggcgttcgag cgcctctacg cgcgtcacca gccggcgatc ctctcgttct
     7201 gccgccatct gaccggcaac cgcgaggatg ccgaggacgc cgtccagcac accttcctcg
     7261 cggcctaccg ccgaatcacg ggtccgggtg acgaccagct cgagctgcgg ccgtggctgt
     7321 tcgtcgtcgc gcgcaaccgc tgcctctcgc tgttgcgcgc ccgcagggag ctcgcggtgc
     7381 cggccgtcga cgccgacagc agggtcttcg acggcctcag cgcggaggtc gagcggcgag
     7441 aggacctgcg ccacctcgtc cgcgacatgg cgcggctccc cgagccccag cgcgcggcgc
     7501 tggttctgtc gcagctcgac gcgctcgact accgcgagat cggtacggtg ctcgatgtcg
     7561 ggcccgagaa ggtcaaggcg ctcgtcttcc aggcgcgcag ctcgctggcc tcggctcgtg
     7621 acgcgcgcga ggccccgtgc ctcgacatcc gccgcgagat cgcgaccgct cgcgggtctg
     7681 cgctccgccg gcgattgctc cgccgccacg tcggccagtg cgacgggtgc agcgagttcg
     7741 agcagacggt gctgcgccag cgacgcgagc tcgcgtcgct gctgccggtg gtgccgtcgg
     7801 ccggcctcgg acaggcggtg ctcgacagcg cgtctggccg cgaagctgcg actgccgccg
     7861 gcgcgggcgg tggcggggct acgacggtcg gagtgggggg tggcgccatc ggaggcgccg
     7921 gcgcgggtgg tggcggcctt gccggtacgt tcggcctggt cggcgccggg ggtgccgcga
     7981 agatcgccgt agtggccgtt gcggtcggcg gcgggggtgc gggcgcggtc gccgccgacc
     8041 tgccgtcacg gatcgacgcc gccgtgctgt cgatcggctc gtccgggcac gccagcggcg
     8101 accggggcgc tggctccgcc gtgcccggcg gcacggacga cggggaacag accacgccga
     8161 gcgccgggga gggagacggg tccgaccggg actcgaccgg cgaccggaaa cacggtttgg
     8221 accggcgcga cagtgccatg cggggtacgg tgaacggcgg acggagcgac ggcgacgcgc
     8281 ctcgcgtcgg cgtgcgggac agctatgcga gcggcggcgt ggctggagac gcgggagacg
     8341 gcggggacga tccatccggg tcccgacccg gatcggcgcc gggtgacgag ctgccgcccg
     8401 ggctcgccaa gcgcgacgag ctgcccccgg ggctgatccg gcgtgacgga cggccgcccg
     8461 gcctcgccaa gcgcgacggc gcgtcgccgg gccggtccgg aggcgccccc ggcgcgggcc
     8521 gcggcgcccc gcccggcgat ggcaacgcgc cgggccgagg caacccggct ggccaaagag
     8581 atacccgcgg gcaggacaat ggtggcggtc agggcaacgg caacggcggg ggtcaggaca
     8641 acgccggccg aggcaacggc agcggcggca gccaaggcac aggtgagggc ggtgccggcc
     8701 aagggaacgg cggcgacagt cccggcaact ccggcggcaa cggaagcggc cccggcaaca
     8761 gcgacggcgg gcctgcgcct cccgagcccg gtcaaggcgg ccctccgggc ggtcaaggcg
     8821 accccggcgg cggcaggggg acccgcggtc cgatcggcca gggcgacggc ggtggaagcc
     8881 ccggccgaag cgacggcgcc ggcaaccctc gctgaacgaa gcgccgccgg ccaggcagat
     8941 cgcacagcat cgcgcgagcc agcggtcgat cagcggcgta cgaggcgcgc gacggctgcc
     9001 atcagctcat cggtcagctc atcggggctg ccgtcgccgt ggccaccgac cacgcagtgg
     9061 cgggcgtggt cctcgagcag gccgagcgag accttgtcga gcgccgcttg gatggcggag
     9121 atctgcgtca cgacatcgat gcagtagcga tcctcgtcga ccatccgctc gacgccgcgc
     9181 acctgtccct cgatgcgacg cagccgattc acgatcttct gtcgatcgcc gctgtagccg
     9241 tgcttcgtcg tggagccgat gatgccttcg tcgttcatgc cgtgcaatcg tagcaatacc
     9301 gggagggggt atgctacagt cgtctttcag ataccccccg accgtataca ggaggaacca
     9361 tatgacgccc gcaaccgccg agctcgagta ttcagtccca ggcgtcagct gcgggcactg
     9421 ccacacggcc atcgccgacg aggtcgagcg cgtggcgggc gtacgccgtg tcgaggtgga
     9481 cctcgacgcc aagcgtgtcg tcgtgcgcgg cggggcgctc gacgacgcag cgatccgcgc
     9541 tgccatcgag gcggccggct acgaggtcgc gccatgaggt ccgcgatgcg catcggggtc
     9601 ttcgcgggcg ggctggcggt cgtctttggc gcggccttcc tagccggcgg ggtgttcgac
     9661 cccgatgtcg aggacggtga gccgaccgct caccgagctg ccgcggccac gcacgagggc
     9721 gatgcgtcgg cccctgcgcg agttcttctc gcgagcagcc gcttcgagcg cggcgccggc
     9781 cagacgctcg ccttccgcgt cgtcgaccgc gagggccgta acgtcgagga cttcgacgtc
     9841 gagcacggcc gccggatgca cctgatcgcc gtgcgccacg acctgaccgg gtatcgccac
     9901 ctccatccgc gcccgaccgg cgacggcggc tggaccgtgg acgtcgactt catggccgcc
     9961 gggccgcacc gcgtctacgc ggacttcgtc acgcggggga agccgtacac gctgagcgcg
    10021 gacgtcgacg tcgcggggcg ctatgccgcc cagacgcttc ccgccccggc gaccagctcg
    10081 gacgcgggcg acggctacga ggtcgaggtc gcgcgtcacg gaggcgagcg ccgcttcacg
    10141 gtctccaggg acggagagcc ggtcgacgac atcgagccat acctcggcgc tcggggacac
    10201 ctcgtcgcga tcgccgaggg cgacctcgcc ttcgagcatg tccaccccaa ggacggcgcc
    10261 accgaggggc gtgagatccg cttcgacgtc gcgttcggcg gccccggccg ctaccggctc
    10321 ttcctccagt tcaagcacga cggcaaggtt cgcaccgccg ccttcaccga gcgtgtgggc
    10381 ggcggggagc cggcgcacgg agacgacggc catggctact gagcgcctcg acctgccgat
    10441 cgcggggatg acgtgcgcat catgcgcgac gcggatcgag aagcggctga accgcctgga
    10501 cggcgtcgac gccgcggtca actacgcgac cgagaaggcg accgtcgcct atgacccgcg
    10561 caccgtggag acagacgatc tggtggcggc ggtcgaggct gccggctacc aggccgtcgt
    10621 gccggtagtc gacgccggcg gtggcgagga ggcgcccccg gccgcggagg actcgacagt
    10681 gcagcttcgc cgcaggttgg cggtcagcgc cgctctctcg ctgccggtgc tcgtgatctc
    10741 gatggtgccg gcgctgcagt tcgactactg gcagtggctc gtgctgcagc tcgcgacgcc
    10801 ggtcgtcctt tggggcgcct ggcccttcca tctcgcggca tggcggaacc tgcgccacgg
    10861 cgcggcgacg atggacacgc tgatctcgct cggcacgctc gccgcctggc tctggtcgct
    10921 gtacgcgctc ttccttggcg aggccggcat gacggggatg acgatgacga tcgacttcat
    10981 ccccgagcgc ggcgccggcg ccgaccacat ctacctcgag gtcgccgcgg tcgtgacgac
    11041 gttcatcctc gccgggcgtt acttcgaggc gcgcgcgaag cgtcgcgcgg gcgcggcgct
    11101 gaccgcgctg ctcgagctcg gcgcgaagga cgtcggcgtt cttggcgagg acggctccga
    11161 gcagcggatc gcgatcgacc agctgaaggc cggcgaccgc ttcgtcacgc gtccggggga
    11221 gaaggtggcc accgatggcg tggtcgagca gggcagctcg gcgctcgaca tgtcgctgct
    11281 gactggggag tccgtaccgg tcgaggtagg acccggctcg gaggtcgccg gcgcgacggt
    11341 caacgtcggc gggcgactga tcgtgcgggc gacgaaggtc ggctcgaaca ccgccctcgc
    11401 gcagatcgcg cggctggtga ccgacgccca ggccggcaag gcgcccgtcc aacggctggc
    11461 cgaccgggtc tcggcagtgt tcgtgccgat cgtcgtggcg ctcgcggtgg cgacgctcgg
    11521 cttctggctc ggcgcggggg agggcgcggc cttcgccttc accgcggcgg tggccgtgct
    11581 gatcatcgcc tgcccgtgcg cgctcggact cgcgacaccg acggcgctga tggtcgggac
    11641 cggccgcggg gcgcagctcg gcctgctgat caagggcccg gaggtgctcg agtcgacgcg
    11701 ccgggtggat acggtcgtgc tcgacaagac cggcacggtg acgtcgggca ggatgcagct
    11761 cgtcgacgtg acggtcgacg ggaccgagcg gcgtgaggcg ttgcggatcg tcggttctgt
    11821 cgaggacgcg tccgagcatc cggtcgcgcg ggcgatcgcg gccgccgccc gctccgagct
    11881 cggtgagctg gcgccggttg cgagcttcgc caatcgcgac ggcctcggcg tcgatggggt
    11941 cgtcgattcc gaccgggcca tcgtcgtcgg ccgccccagc ctgctcgagc agcgggggct
    12001 ggagcttccg gacgggctcg agcaggcgcg ccgcacggcc gaggccgacg gacgcacggc
    12061 cgtcctcgcc ggctgggacg gccgggtccg ggccgtcctg gccgtcgccg acacgcccaa
    12121 gccgaccagc gctgcggcgg tccaacggct gaagcggctc gggttgcgcc cgctgctgct
    12181 gaccggcgac aaccgtgcca ccgccgaggc cgtcgcgcgc gcggtcggca tcgacgaggc
    12241 gatcgccgag gtgctgcccg cagagaaggc cggcgtggtg cggcgactgc aggacgaggg
    12301 tcgcgtcgta gcgatggtcg gcgacggcgt caacgacgct cccgcgctcg cccaggccga
    12361 tctcggcatc gcgatcggga cagggaccga cgttgcgatc gaggcgtcgg acctgacgct
    12421 ggtcagcggc gacctgctcg ccgcgcccga cgcgattcgg ctgtcgcgcg cgacgctgcg
    12481 cacgatcaag cagaacctcg gctgggcgtt tggctacaac gtcgccgcgc tgccgctggc
    12541 cgccgccggg ctgctcaacc cgctgctcgc cggggcggcg atggcgcttt cgagcgtgtc
    12601 ggtagtcgcc aacgcgctgc gcttgaggcg gttcagggcg gggcagggga gcaagcgatg
    12661 aacgggcaca gccaccacgc cgccgtggcg agttcaggtc gcgcgctcga cccggtcgcg
    12721 ctgagcgcga cgctgcactg cctgaccggc tgtgcgatcg gcgaggtcgt cggcatggtc
    12781 gtgggcaccg cgctcggctt cccggagctc gggacggtcg ccctggcgat cgccctcgcg
    12841 ttcctgttcg gctacagctt gaccagcctg ccgctgctgc gggccgggat ggccttccgc
    12901 gccgtggtcc cgatcgcgct ctcggtcgac acggtcagca tcgcgctgat ggagacggtc
    12961 gacaacgcga tcatgctcgc gattccgggc gcgatgaagt cgggcgccgg cgacatcctg
    13021 ttctggggcg ccctgtcggt cgcgctcgtc gtcgccggca tcgtcgcctt ccccgtcaac
    13081 cgctggctga tccagcgcgg caagggccac gccgtcctgc acgagaccgg catccacggg
    13141 gggccgccga cgaagctggt cgccgcgatc gctgtcctcg cggctgtctt tggaactacc
    13201 gtcctcgtcg ccgaggcgct cggcggatag ccatggcagc cctcgcgctc ggcgcttacg
    13261 cgctctacat ggcactcgcc ttcggccttc gaaccgccgt gcagctgcgc cggaccggcg
    13321 cgtccgggtt cgagggcgtc agcggccgtc ccgggtcggc agagtggtgg gccggtgtgt
    13381 tgttcgccgt cgcaatcgcg gtcggcgtga cggcgccgct gctcgcgctg ctcggcatcg
    13441 tcgagccggt ccgcgcgttc gacgtgaccg ccgtccacgc cgtcggagtg gtcctcttcg
    13501 gcgccggcct cgccggcacg ctggcggccc aggtcgcgat gggcgactcg tggcggatcg
    13561 gcgtcgacga cagcaccgtc accgagctcg tcgtagacgg cccgttcgcg gtcgttcgca
    13621 atccgatctt cgccgcgatg ctgccggcct gcactgggct cgcgctgatg gttcccagcg
    13681 tcgtctcctt cgtcggcctg gcggcgctcg tgatcgccct gcagctccag gtgcgggtcg
    13741 tcgaggagcc gtacctgctc cgcgcccacg gttcccgcta tcgcgagtac gccgcgcgcg
    13801 tgggccgctt cctgcccgga atcgggcggc tgggggtgga ccggtgagcc gaatccctga
    13861 ccggcttcga ctcgcgaccg cggtctgagg ggagcgccac ccgccggtgg gacgaaacac
    13921 acccgacgcc gcagggggcc acagcgccgg gtgctcgttc gaggttagcg gccgctcgca
    13981 cggaggacga gcgcccgatc agacgggctc ggaggcggtc tcggctgccg caggagccag
    14041 cagcctgcgc agcacaggcg gcacgagcag cgtggtgagg atcgacattc cgacgatcac
    14101 cgcgaacagc tcgtcgtcga tgaccccggt tgtgcggccg atgccggcga cgatgatccc
    14161 gacctcgccg cgcgggacca tcccgaagcc gaccgcggcc gcctcgctgc gcccgagcga
    14221 gcgcgcgccg agccaggcgc cgccgaactt cgtcgcaatc gcgagcacgg tgacgagcgc
    14281 caacaggccg agcgcccccg ggtcggcgag tgcgccgagg tccagctcgg tgccgatgaa
    14341 agcaaagaag aacggcggga agaatgcgta gagcggcgcg acctcgtcct cgacggggtg
    14401 ctgctccttg gtctcggcga ccatcaggcc ggccaagaac gcgccgatga tcgccgccag
    14461 accgatctgc gcggcgaagg ctgccaggcc aaggcagagg atcacggctg gcagcagggg
    14521 ggactccgag aagcggggcg ccttgaagac ctgaggccag cgctgggtga cgcgcgtgcc
    14581 cccgagcgcg aagaagccga cgaagccgag cgagatcgcc agcacccaga ggatgcccgt
    14641 gacgtcgacg ccgccctcgg cggccaagcc gaccgcgacc gagaggatga tcagcgcgag
    14701 cacgtcgtcg acgatcgccg cgccaaggat cgtgcgagcg acgcgcgttc gcagcacccc
    14761 gagctggatc agcaccgccg aggtgatgcc gacgctcgtg gccaccagcg cggcgccgag
    14821 gaacaccgcg gtggccgcgt catcgccgcg cccaaggccg aggccgacgc cgccggcgag
    14881 cggcaggacc acgccgagga tgccgaccag cagcgccgtc ttgccgacct cgcgcagctc
    14941 cgagaccttc gtctccaggc cgacccagaa gagcaggaag accacgccga gctcggcgaa
    15001 gacctcgagc accgcgtccg gctcgacgaa cccgagcagc gacggcccca cgatcacgcc
    15061 ggcgaggatc tcgccgacga tcgtcggctg gccgatacgg cggaagacct cgtcgccgat
    15121 cttcgcggcg agcagcagca cgaacagcgc gacgagcgtg tcggcgacct ctgttgccgc
    15181 ggcgatcgga agggcgtcga tcaggaggga gccaccacct cgagcacgcc cgccttggac
    15241 ggcaaggtgt cggccgcggc gggctggatg gcgatggcgt ggcggccggg ctcggttgcg
    15301 cgcacctcga agaccgccgg cgtcagcggc tccgcgaccc cggtcagtcc gaggctcggt
    15361 attcgcacct cagccggcgt ctggacgttg acgagcacgg tggccggctg gcccaccgtc
    15421 agctcatgcg tctgtggttt cgcggccggc tcgaactcga tcgtcgtcgg tggtggcgac
    15481 tcgggcgtcg gctccgcggc tgccgatccg gggcgcgtgc cggtgccgcc gctccgatcg
    15541 gtcgcggacc gctcgtcgcg gccgtcaggc ggacggacga tcgagctggc gatcgccgcc
    15601 acgccgagga cgaccacgaa cagcagcagg gcgcgccgaa gctccacggc gccgatggta
    15661 ttcgccgcgc agaagctgca ccgacccggc tagggcgctc gcggccgtgg ccggacggta
    15721 gagcaggagg gccaggccgg tggcgagcgg cagcgcccat gccatgtaga gcgcgaagag
    15781 aacgcccgcg tgatcgcgcg agtcgccgaa catccggtcc tcgatcggcc aaaagcttgc
    15841 gctcacgagg atcaagacgg ccatcgtgag atagggaaca cccggtcgtc gcacgctctc
    15901 ccacgcgagc agtgagagca cgagcggcac gtggtagtag tcgttgttcc agttgtcgag
    15961 caggcagcgc agcaggaaca gcagcgccag cagcagcagc gcgtcgtcgg gtcggcggtc
    16021 cgcccggcgc catagcagca gcgagagcgg gagcgcgacg gcgacgatgc ccgggtgtga
    16081 caccgtcgcg acccattgcg gggccatcca gcgggggccg aacggggcga cggcgacctt
    16141 gtcggcgaac ggccaccaga cgttccaggg cgtcgtcgag gtcccgatcg cgtcctgccc
    16201 cgcggccgct cgcgccacgg atccgaactg accggggtcg ccgagcatca gcggcagcgt
    16261 gagcgcggcg gcgatggcgc cggcgacgag tgcgaccgtc agtcgccggg acggcgcggc
    16321 gagcaggacc ggcagcaccg ccagcagcgc ccactgcttg gtcgcgagcg ccaggccgag
    16381 caggaccgcg gccgtcagcg cgcgctcgcg catggcggcc agtacggcgc cgacgcagag
    16441 cgccccggcc aggagctcct ccggatggcc ccagtggagc gcctggaagg tcagcgggtt
    16501 gatcacggcc agtccggcgg cgaggccctg cacggtcgct ggctgttcgc gctccgcgag
    16561 caggcgggcg agcgtcaggc cgaggaccag ggtcgccgcg aggcatggga cggcgccggc
    16621 gaagtacacg gcctcgacgc tctggtggaa gacggcggcg acgaacggcg cccgtaggag
    16681 catcgagaag ctgcccatca gcggctggtt ggctacgaac ccctggaggt ccccgcgggc
    16741 cagcgcgtcg atcgagtcgc cggcgtcgtt gaggtagtcg agcccggcct ccatcgacgc
    16801 ggccgcccgc gccgtcagcg caatcaggac caggacggtc agagccgcga gtgcgcgccg
    16861 catggaccgg ggatcggcgc atccgcgccg gcccttgaag cgcggggagg acgttgcggt
    16921 tcagagaggc cgcgccgcga ccgctggacg aatggacgag ctccagggcg gatcgccgct
    16981 aaaggaaccg cgacgggggg tcgatcagga ccgagccagg aaatcgccgg ggcaaccgcg
    17041 gtgattggcg acccagaccc aaccagtcac ctcacaagga ggaagaccac atgctgcgga
    17101 agctccgcga gcgaatccag aaggacgagt cgggcttcac gctcatcgag ctcctcgtcg
    17161 tcatcttgat catcggcatc ctggcggcga tcgcgctgcc ggcgttcctc ggtcagcaga
    17221 cgaagggcca ggactccgag gccaagagcg atgctcgaaa tgccgtgtcg caactggagt
    17281 cgtgctacac cgacgctcag acctatgtcg gctgtgagac cgacgccgcc gttggcttcg
    17341 acaccctcaa cgtcgagggc gcgagcatcg agaccggggc ggacaccttt accgtcacgg
    17401 cggtatccaa gtcggacaac atcttcacca tttccaaggc agcggatggc accacgactc
    17461 gtggctgcac agtcgttggc ggcgacgacg gcggttgcac cgagagctgg accggctctg
    17521 gtagcggaag ttagccgcac cgcggagcaa ggcgcttcag acaggaaagg gcgggtcctt
    17581 cgggcccgcc cttttttgtt ctcccgacac ttcccatcgg gcgccgccgc tgcaaggatc
    17641 tggccgaaag atggacgccg acggcggtag cgctgtcggt tccgccatag tgcgcgcgtg
    17701 ctcgtcaagg gtcgtcccga acggccgatg gcgtagcgat ggcccgcgcg agaatcggct
    17761 cccaaagcgg cttcaccttg gtcgaggtga tggtcgcgat cgtcctgctg ctggtcggcg
    17821 tgctaggcgc cgtatcgctg atcgaccgcg caaacgcgca gacttcgacg accaaggcgc
    17881 gtgagggcgc tacggcgctg gcgcgctcgt tgctcgagat ctcgcgcggc gttccctacg
    17941 ccgagctgac ctccgagcgc gtgctggccg aactcgacgc gcgcggagcg ggattcgcgg
    18001 atggcgatgc cgccgccggc caccagatcg ccagccgcgg cttcgtctac acggttgagc
    18061 caacggtctg ctcgatggac gacccgaagg actcgctcgg tccgcacgac gagctcggcg
    18121 tgacgttctg cgccaacagc gacgtcgccg ccggcggcgc gaccaccgtc gaccgcaacc
    18181 ccgacgacta ccgccgcgtc gtcgtccgcc tgagctggcg cgtgggcgca ggcgggccgg
    18241 tccagagcct cacgcagacc ggcatcgtca cgaacccggt cggcgggctc gggcccaacg
    18301 tgctggcgct gacgccgatc agccccgcca cgccaacgat caccggcgac gaccagcacg
    18361 cgaactacga cgtcctgacc tccgcggcgg cgcaggacgt cgcctggtcg gtcaacggcc
    18421 gccggatggg cagcgccgag ggcgcgggga ccgagtggga cttcacctgg gacctcggcc
    18481 ccgtggacga tccagccgtc tacgactgca cctacgtcgt gcaggccgag gcgttcgacg
    18541 acaaggcgcg cgccggcgcc ccacgcgcgc tgacggtcac cgtcaaccgc cgccggccgt
    18601 tcgggccgcg cgacttcgcc ggcggcgcca acgggaacgg cggcggccgc ggcgacgtcg
    18661 acctgcagtg ggacgcgaac ctcgagtgcg acgtccagcg ctacgaggtg taccgcgagg
    18721 tcgacggcgt gcccgacgac ggcccggtgt gcgaggtcga tcgaggcgag cgaaccgagt
    18781 gcgtcgacga ggcagcgccg tcgaccggcg agctgaccta ccgtgtcttc gccttcgacc
    18841 tcgaccgcga cgggaacgag cggcgcgggg atccgagccc gcatgcggtg gtcgttcccg
    18901 atccggtcat caacgacgcg ccggaggctc ccgccgggct gacggcgtgc accggcggcc
    18961 agttcgacgg cccgtgcctc gacatcgagg gcaacccggc atccgacggc acggccgtgc
    19021 tctcctggcc gcccgtcgcc aatcccgaca ccgacggcga cgcaatccgc ttctaccgcg
    19081 tctaccgcgc cggcatcggc gctgccgcgc ccacctacgc cgaccggctc gacgtcctct
    19141 tcccggtgtt cgacgagcag ggcgagcccg tcgatccgct cgtcttcgtc gacgggacgg
    19201 ccagcggccc gcaccgctac tgggtgaccg ccgtcgacga gcgcttcgcc gagtccggcc
    19261 cggtcggccc ggtcgagtgg ggtgccccgt gacgaggatc gtccgcgagg agagcggcct
    19321 gatgctcgtc gagctgctcg tcgtgatggt cctgtcgggc atcgtgctgg gcgcgacgct
    19381 gaccaccttc aaccagttcg agcgcacgac cggcaccaac cagcgccaga cagaggcgca
    19441 ggagcaggtg cgcgtcggcc tcgccgcggt ggctcgcgag ctgcgcaacc tcgcgagccc
    19501 gacgaacgag cggccgcacg cgatccggca cgccggggcg agcgacctcg tcttccagtc
    19561 gatgagcagc acggtcacgc gacgggtgcg ctactgcctc gacgctgagc agcggacgct
    19621 ctggcgccag gtccagctcg agccgttcag cgatccctcg cagaccgcct gccccgacgt
    19681 cggctggggc agcgagcgga cggcgatcgc cgacgtcgtc aacggcgcgc ggccggtctt
    19741 cggctacaac gtcgaggatc cgatggggat caccgagatc tcggcgacgg tctgggtgga
    19801 cgtcaacccc ggcgtcgcgc cggccgagac ctcgctgcag acgtcgatct tcctgcgcaa
    19861 ccagaaccgc agcccgaccg cggtctcgac ggcggagctg agcggctcgt cgatcgtcct
    19921 caacggctcc gagtcgttcg atccggaggg ccgctctctg cgcttctatt ggtacgaccg
    19981 agccgtgtcg ggccacgagg cctgcggtga ggatctcccg tccgacctgc cgcaggacgg
    20041 ctgcgtcgcc accgggatcg tggccaccta cacgccgccc gagccgggct cgcgcagcct
    20101 ctacctcgtg gtcgccgacc cggccggtct gaccgaccag gcgcccgacg agtcggtctg
    20161 cgtcccggga ggaccatcgt gctgaggctg agcgagcgtc tgcggacaga gcaggacggc
    20221 aacctgctgg tgatcgcgat gacgatgatg acgctgatga tggtcacggg gttcgccacg
    20281 ctgtcgatcg tcgacaccca gaccgacgtc gcgatgaagg agcgccagca cgagtcctcg
    20341 ttcaacctcg ccgagggcgt gctcaacgcc cagacctacg tgctcgcgcg ccgcggcacc
    20401 ggcgacgccg gggcgtccca gttcccggat gcctgcgtca acgggacgac ccactccctc
    20461 tgtccgagcg aagagcagct cgcccgcagc tacagcgcgg cggtccaggt cgactacggc
    20521 gacgacacca actggaccac gcgcgtgcgc gacaacccca acggcacctt ctacgacccg
    20581 accgcagtcg cggccgcggc ccgctacgac gccaacggag atcgccagct ctgggtgagc
    20641 gcggaggcga ccgtgcgcgg tcggacgcgc cagatcgtcg cgctgatccg cgtcgaggac
    20701 cgcccggtga cattcccgag ctatgcgctg ttgggcggga tgttcgcgac ctcgaacagc
    20761 ggtcgcggca cgatcgtcga cgcgaccgac ccgagctcgc tgggcatcgc cgtgcgctgc
    20821 gtcggcgagc cgagcaagca gtcgagctgc ctcaagttcg acccgaagaa ggaccagctg
    20881 aatccaccca aggcctatac caccggagtg cccgacacgg cggcgatcag cctggacgac
    20941 ctgcaggcgc tcgaggagta cgcgcgcggg agcgggacct actacgggtc gtgcccgggc
    21001 ccgaacccga acggtgccgt cgtggtgatc gagtccgggg actgcccgtt cagcaacagc
    21061 tcgcctgccg cccccggagc gtcgaagtgc tgcaacacgc cgacccggcc ggggctgctg
    21121 atcgtcaagt gcggcaccgt cacgctcaag ggcaacatcg agttctacgg gatcatctac
    21181 atgccccagg gcgattgctc cttgaccggc tacgtcgtca cgacgcaggg cacctcgctg
    21241 atctccggcg gcgtgatcat cgacggcatg gaccgcggcc tgctcgccgg ctcaagcggc
    21301 gacaacatcg tcttcaaccc gctcgctttc cagaacgtgc gcgcggccgg aacggctggc
    21361 gtcgtccaga acacctggcg cgaggtcccg gacgacctgt agacgccgcc cgtccgcact
    21421 cagcttttcg agcgccatgc cgatatcggt tgcgtggtcg gtcacgcaat cgccttcaag
    21481 ctccgtgacg agcgcggcct gacgctggtc gagctgctgg tcacgatcct ggtcatgggg
    21541 ctggtggtga ccgggatcct cggggtcctc gacaccagcg cgcgcatcgc cccgaaggac
    21601 gtcgagcgcg ccaacgcgat cggcgaggcg cagacgggcc tgaaccggat cgtgcgcgag
    21661 ctgcgccagg cctaccggat cgtcggctgg acgccgagct cggtccagat gcgggtcaac
    21721 atcctgcgcg acgatccgtc gaccgccggc ccggactatg ccaacgtcac cgtggactac
    21781 agctgcggcg gcgacccggg ccgctgcgtg cggcgcgagg cgccggtggg cacgaccctc
    21841 ccggccaccg ggatcgtcgt ggtcggccgc gtgctgaacg ggggcgccgc gccaccagag
    21901 ggccgccacg tcttcgattt cgaccagagc cccgaccgca gcgtcggggt caccgccgcc
    21961 agcgtgcgtc cgacctacgt ctcggtgcgg atcgaggtgc cagcggccgg cgagcgcggg
    22021 agcggcggcc accagcacag cgtcgtcctc gacgacggtc tgtacgtccg caacgtcggc
    22081 ggggtgcact gaggtgggcc gcgcgccccg caacgccggc cggcggtcgc cgcggtccca
    22141 ggacggcttc acgctgatcg aggtgatggt ggctctgctg ctgctgacgg tcgggatctt
    22201 cgccgtcgcc acgacgttcg acttctcgcg cgcttcgacc agccagtccg agcttgagac
    22261 ggcggcggtc gaccgtgccc agcgcgagat cgaggcgatc cgctcgctcc cctacgagca
    22321 ggtcgcccac ccgctcggag gcatcgcccc cgcggcgggc gacgaggccg atccggcggc
    22381 gagggtaagc ggagcgagct ttgcctggga ccgctccaac gacgccgcct ccgagccgct
    22441 cgtgatctcg gcggacggcg cggtcccgat gaagcagacg atcgcccggg gcgccggcga
    22501 ccgcttcggc tacacgattt ggcgcttcgt gacggcaacc gacgagccgg cttgcgtcgc
    22561 cgcagtggag tgccccgacg acgggggcgg gtacaggcgc gtgacggtcg tggtgcgggt
    22621 cagtggcgtg ggggagacac tcgcaccggt ctggacctcg acgacggtga tcgaccccgc
    22681 cgcggccgcc aacaacgacg gaaccccgca gacgctctgc cagaagcccg acggcagcgg
    22741 gctggagctg tgcagcgagg aggtcgacgg cacgtcgatg gacctctatc tgaccaacac
    22801 accggctggc ggccacgacg cgcgggtcga gccggcggcg tccgggccgc tgcacaagac
    22861 ggtcgcgatc ccgccgtcct gccacgccgg cgccgcggtc ggctgccccg tccccgacct
    22921 gatggtgggc gagccgctcg cacagctcgc cgaggaggac cccgtcccgc cgctgcgaac
    22981 ctttgcggcc gacgtcgagc cgtctgtcgc ggccggccgc ccgctgctcg aggacgtcgc
    23041 ctgcgacgcc acgccgaccc ggcacggtgg cgacgacgtg aaccttcgcg gcgcctactg
    23101 ggtctcgccc gtcctcgacg cgccgatcca cctccgcggg gtcggaggtc tgtacctcta
    23161 cgcgcgcacc tggagcgaga ctgccgccga cgtcacgctc tgcggcatcg tctacagggc
    23221 gccggctagc gtcgccaacc cggtcctgag cccgcctcat gagatcggcc gcatcgatgt
    23281 cacgaccccc tggccggaga ccttccaacc ggtggcggcg accttcgact tcgacctcga
    23341 tcccggtgac cctcacgcga ccgtggaggc ggacgagcgg atcggcctgc gcctctgggt
    23401 cgccgacggc tcggcggacg agatcgtcct gctctatgac cacccgctca atcaatcgag
    23461 gctgaccctc gtggtggagg agaccggatg acctcccgcc ttcgcatctg tcgccgcctg
    23521 cacaccgagc aggacggcgt ggcgatcgtc gtggcgatcg gcgcgctcgg cgtgctgctg
    23581 atcctgtcga cggtcgcgtt ctcggcctcc tcgcagctca gcgacacgtc gaaggtggac
    23641 cgcagcgcgc gcagcgcgtt tcaggcggcc gaggccgggc tcgacgtcgc cacctaccgc
    23701 atcaacaacc tgctgccact cggcggcggc gccggctgcg tcgccggcgt cgaggacgcg
    23761 acggccgatg gcagctgcgt cgcgggcgag cagctggccg acggcgcttc ctggagctac
    23821 gaggtcaccc cggacctcga cgaggagaac tacgacgagc tgctgccggg cggccgctgc
    23881 gccggcttcc cgatccagtt cgacgccgac gatctcgacc tcacgttgcg gtcgcgctgc
    23941 atcaccgcga cggggaccgc ccatgggcag acccggcgag tgcaggcgcg gctcgccctg
    24001 ttcaccggga cgagcagcga gaccccaggc acgacgctgt tccccgtcgg cgggatcatc
    24061 gggctcgacg gcgtcacgct cagcaacgcg gcgcaggtca gcggcagcgt cgcgtcgaac
    24121 gggcaggtcg agctgagcaa cgcggcccgt gtcaccggcg ccgcgatcct cggccccggc
    24181 gcccccgcgc cggatgtcgg caacgccgcg acgatcggac agggcacggt ctaccgctcg
    24241 ccagcggagg gaccgtgggt gctcgcccct gtcgacatcg gcaacaccgc gaccgtcaac
    24301 cgcaacggca acggcatcgg ctggacggtc gagagcggga ccgccaccta cagctcggca
    24361 taccgtgagc tcgagatcaa gaacgccgcc cgactgaact tcagcggcgg cgactacaac
    24421 ctctgccgtc tcgaggcgaa gaacgcgacg aggttcaccg tcacgccggg ggccaaggta
    24481 cgcctgttca tcgactcgcc gacacgcccg ggctccggct gccgccccgg caccggtacg
    24541 ttcgtcgcga agaatgcgtt cgacctcgag gccgacgaga agtcggccca gttccagatc
    24601 tacatctacg gcggcgacgt cgacggcaat cccgccttcg aggtcaagaa ctcgagtggc
    24661 ttccacgcgc tgctgcacgc accgctcgcc tcggtcgagt tcaagaacgc cgcctccttc
    24721 tggggggcga tcaacgccaa gcgggtcgag ttcggcaacg cgagcagctt ccactggccg
    24781 gccgagggca tcagcggact cactcccgac acggacacgc cccagaacac cctgacgttg
    24841 ttccagcgca gcgcctggcg cgagtgccga agcgcgccgt cggaccccga gaatccgcac
    24901 tcgggctgct gacggcgcgc gcggtggcgc gctgcgacga ccggcgccgc ggatagcgtg
    24961 tcgcgccgcc atgcctgacc cgatcgcgat cgccgtgctc gccctgctcg gcctcgtggt
    25021 cggcagcttc ctcaacgtcg tcgtccaccg gctgccgcgc ggcgaatcgc tttcgcaccc
    25081 gcgttcgcgc tgcgtcggct gcggcaccca gatcgccgcc tacgacaacg tcccggtcct
    25141 ctcatggctg atgctgcgcg gccgctgccg ccactgcggc gagccgatat cgccccgcta
    25201 cccggcgatc gaggcgctga ccggcgtcgc cttcgccggc gtggcggcga tcgcgggcgt
    25261 cgacgccggc ctctggctcg cgctgccgtt cgcggcgctg atgatcgcgg tggccgcgat
    25321 cgacctcgag caccggatcg tccccaaccg gctgctcgcg cctgccgccg tctgggccgt
    25381 agtcgcatgg gcggcggtcg atctcgactt cctgccggag gcgctggccg ccggtgcggg
    25441 cgcgttcgcg ttcctgctgc tcgccgcgct cgcctatccc gccggcatgg ggatgggcga
    25501 cgtcaagctg gccggggtga tgggcctgta cctcggcctg tcggtggtcc cggcactcct
    25561 ggcggcgttc ctcgccggct cggtcgtcgg gatcgcgatc gtcttgcgcg acgggcgcga
    25621 cgcgcgcaag acgggggtcc cgttcgcgcc cttcctggcg ttcggcggcc tgatcggcct
    25681 gttggccggg agcgacgcga tctcgctcta cagcgaccac ttcctctagt tcgcccgcgg
    25741 ttcaagccgc cggcggacgt gccgataagg gacgggaccc tcccatggcg ctctccctac
    25801 gcaagaactc cgacggcggc accgtcgggc tcgacatcga cggccgcttc gttgcggccg
    25861 cgcgcatgca tggcggacgg ctcgcatcgg tggcgagcgc cgagctgccg gcaggtgctc
    25921 tcgccgacgg cgaggtcgcc gaccgcgccg cgctggccgc ggcgctgaag gagttcgcag
    25981 ccgccaacaa gctgccgcgc cgcgtgcgcc tcggcgtctc caaccagcag atcgtcgtgc
    26041 gggtgctgga cctgccgcgc atcgacgacc cgagcgagcg cgacgccgcg ctgcgctttc
    26101 aggccgccga ggcgatcgcg atgccgctcg acgaggcggt cctcgaccac cagatcgccg
    26161 gctacaccgg cgggggggac agcggcgagc ggatgcaggt cgtggtcgtc gccgcccgcc
    26221 gctcgatgat cgacgagtac ctcggcgcgg ccagcgacgc cggcctcaag gccgacggga
    26281 tcgacctgga cgccttcgcg ctcgtgcgca tgctcgcgac caccgacgcg tcgccgctct
    26341 ccgcggtcgg agacgaggac ggcgcggtgc aggacgggct cgccgcgggc ccgcagaccg
    26401 ctcgggtctt ctgccacctc ggcggcgtct ccaacctcgc gatcgcactc ggctcctcgt
    26461 gcttcttcac gcgcgcgctc gcgacccact tcgacgacga ggcagcgggc tcgcgcctcg
    26521 ccgacgagat ccggatgtcg atcgactact acatggccca gccgggcgcg ctcggggtcg
    26581 gcgacgccgt tgtctccggc gagggcgccc gcgacgaggc gctggtcggc gagctcgcga
    26641 cccagctggg gctgccggtc gccgcggctg agccgctcgg cacgctcgac gcgaccgccg
    26701 tgccggccgg cgaggaccgc tatcgctaca ccgtcgccgc cggcctcgcc ctgggggtgg
    26761 cggcgtgagg cccgtcaatc tgctgcccga gtcccagcgc ctgccgtcgc tcgcggtggg
    26821 acgccggcgc tggg
//
LOCUS       DATABV010000210         9028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_69507, whole genome shotgun sequence.
ACCESSION   DATABV010000210 DATABV010000000
VERSION     DATABV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69507"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="VHF88_09680"
     CDS             complement(<1..1585)
                     /locus_tag="VHF88_09680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2162075.1"
                     /translation="MGRALAIAAIALVAALGVVAAPALSTKPYRPEPVDFAVAAGGVL
                     GDPDPSEGVVSEPIEAPKRFNLVGLTWSDGAPVEGHGSEPALAMRTRTDDGEWSRWAP
                     VTAHAEDGPDPGSDEAHAAGMSNPVWAGEADWVQYRSSERLGGVRLHFVNTDGTATAA
                     DSVKSALRGAANTAVTTAAGLLSAELAGAQDTQPEIVPRSSWAGNDCPPRTGAVYGEV
                     RAAYVHHTVNINDYSRAEAPQVVLGICRYHRNTNGWNDVGYNFLVDRFGTLYEGRAGG
                     VGAAVVGAQAEGFNSYSTGIANIGTFSSVPQSDEALAAMARLIRWKLPLHGYPTNGTA
                     VMQSAGGGTNRYSAGTTVRVPRVLGHRDTNATECPGTTLYSQLEELRSMVAGVAPTGV
                     GTNIAAELDAPRSAIDYGDEATVSGTLTTRDGAPVPGQPVTVQARVDGTWRTSSTPVT
                     AADGSFAATVKPRVNRQLRVRFAGSGELRSSVTPTLTLAVRPLVGLRLLRAQPARHAA
                     RARAPRGRVAVVAGERVILR"
     gene            complement(1661..2374)
                     /locus_tag="VHF88_09685"
     CDS             complement(1661..2374)
                     /locus_tag="VHF88_09685"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2162076.1"
                     /translation="MASRREQKEQLRKEREQREAAAKAGQRRKQLIGYGVGGALVLVA
                     VIVAVALLGGGGGSSSASGDVLPDGGSYPEPRDDVPVAAAAKAADCELKSYPAESREH
                     LADLSQKVNYASKPPTSGRHYQVAAEDGAYEDPPDVKELVHTLEHGRILIWFKRNLPA
                     DQRAALKKYYDDDSYQMVLVPDMTGMKYAVAATAWNRDPQPNGTGQLLGCAKYNEDIF
                     TAIESFKDENRSRGPEPVP"
     gene            complement(2388..3233)
                     /locus_tag="VHF88_09690"
     CDS             complement(2388..3233)
                     /locus_tag="VHF88_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2162077.1"
                     /translation="MAPRTRSFRHDGHRLVYDEYGSGDRPVVLMPGLLFSRKMHGPLA
                     EELAARGHRVLCLDLLGHGDSDRPEEMWEYSMTAFGGQAIGLLDHLGIEQAVLGGTSL
                     GANSALEAAALDQSRVKGLLVEMPVLDNALLGAAIGFTPLLVSLTFGAPITRPLSRAA
                     RLLPRRGPLLGKMLLDWVSQDPKPSASVLQGLFFGRVAPPREVRRTLKPPTLVIGHPR
                     DPIHPFSDADMLVHELPNATIVEASSIVELRITPERLTGEIATFVDGCWSLPHIGVAR
                     AARAH"
     gene            complement(3247..5394)
                     /locus_tag="VHF88_09695"
     CDS             complement(3247..5394)
                     /locus_tag="VHF88_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2162078.1"
                     /translation="MFDALAGLADRRGKLVVVLAVLFFAVAGAVGGGVADKLHPYGAD
                     DPDSETYEAREKLREAGHRATSVVVLIDGKVESPRTERRVAQIQAQLQEDPDVASVAG
                     VGTTRQLDFVAEDGSAQYLAVTLKPTDDKEWQAAAERIADDLAGTPGVKVGGPALAQA
                     QVNDQVEADLQRAEMLAFPLLFLLSLLFFRSLVAAALPLLVGGLAIVGTFLTLSIASE
                     LGSISIFALNLTTGLGLGLAIDYSLFVVSRYREEIAKDGPGLAAMRRTMATAGRTVLF
                     SSLTVAAALASLLVFPQRFLYSMGLGGAAVALLAAAVSLIVLPAVLTLLGNRVNAGAP
                     KFLQRRAEADARPDSAGFWYRLSRFVQRIPGRIAIAATVLMLTLGIPFWSIEFTSVDA
                     QVLPQDKSARQVDDVLRSDFPPFRDTPIDVLVSGADSDPRKIAAVSEQIARLQGIDRI
                     FAPTALESGDTLIQAISATPHISEASQETVREIRGLDPPPGVTVQVTGGTADFIDFQS
                     SLVDHLPTAVAIVVIATLLILFLMTGSVVLPLKALLMNVLNLSAVFGILVFIFQDGRL
                     EGLLDYTSQGALEQTMPVLLFAVAFGLSTDYAVFLLSRIKEARDKGASDTDSVAIGLE
                     RTGRIVTAAALLFAIAIGAFATSEIIFIKQNGIGTALAVLLDATIIRALLVPSLMELL
                     GRWNWWAPRPLRRLHDRFGLSETPTGEPRPAEA"
     gene            complement(5396..5956)
                     /locus_tag="VHF88_09700"
     CDS             complement(5396..5956)
                     /locus_tag="VHF88_09700"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2162079.1"
                     /translation="MEVGKTGYVILGMLYKGPKSGYEIKAKADVSTRFFWAISYGQIY
                     PELRRLEQAGLIEGEADAGNGRQRRVFSITASGEDALREWLKRPGELHSELRHEGVLR
                     LFFADALDRDERIELLNQIRVKHTRMREELAGIEPAATALAEETGDAMPLLTLETGIA
                     YQQFFVDQCDRLERRLAETESTTGGS"
     gene            complement(6040..6990)
                     /locus_tag="VHF88_09705"
     CDS             complement(6040..6990)
                     /locus_tag="VHF88_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2162080.1"
                     /translation="MRLEGIHHITAITGDAAGNVDFYARVLGLRLVKKTVNQDQPSVY
                     HLFYADEQGSPGADITFFEFPGAARGRAGAGMVHTIVWRVASEAALDFWAGRLAAEGV
                     SIERGEGSLKFADPEGLGHELLVADVPDQPLIADHPEVPRELALQGFHAARAYTRDPE
                     ASRPLIEEQLGFGPVDEDPAHPGDMRATRAYEVRGASRGGLWIYDEPPSEPGREGAGT
                     VHHIAWASEMDDHEAWRERALAGGARATEVIDRFWFRSIYFREPSGVLFEIATLGPGF
                     DADEDPAHLGEKLVLPPKFEPLRERIEATLTPIENPRASG"
     gene            complement(7023..7478)
                     /locus_tag="VHF88_09710"
     CDS             complement(7023..7478)
                     /locus_tag="VHF88_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2162081.1"
                     /translation="MTSPPPDNAKSIDVLLVEDDPGDTLMIREAFEDNKVRNRLTSVT
                     DGVQALDFLRREGEYAGAPRPDLILLDLNLPRKDGREVLAEIKSDDDLATIPVVILTT
                     SQAEEDVLRSYKLHANAYVTKPVDFERFIEVVRQIDSFFVTVVKLPNGD"
     gene            complement(7475..>9028)
                     /locus_tag="VHF88_09715"
     CDS             complement(7475..>9028)
                     /locus_tag="VHF88_09715"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2162082.1"
                     /translation="PRPAPAGGRRGNRFRVNQWFAIATGLLSLATLTGIVIGVVAIVR
                     LSDERELVVDHLDPAAVESLRLANALVSEETGVRGYVLAGDATFLAPYRAGRVEERRA
                     VRRLRVQLRGAGLDELSSDLAEVERRAHAWRTRYARPTIARVRQDGAGAIGSAGVERG
                     QRLFEALRRALERQEADLARAREDGRDGLAGAATFLSASFGGIALLVVAALATTLIML
                     RRTVTLPIGALARRVRRVAAGEFDAPVRGAGPRDIADLAADVDSMRQRIVAELASLRD
                     AHAQLDEQARDLERSNAELEQFAYVASHDLQEPLRKVASFCQLLEQRYRGQLDDRADQ
                     YIDFAVDGAKRMQQLINDLLAFSRVGRTSGDLTPVDLGETVRRALADLDSAVADSGAT
                     IEVGELPMVRGDASLLTLVFQNLIGNAIKFRSDAPPEVRIASEREGDDWVVTCSDNGI
                     GIEPEYAERVFVIFQRLHGKDQYAGTGIGLAMCRKIVEHHGGRIWLDPGAGPGTTFRF
                     TLPALEESR"
BASE COUNT         1269 a         3215 c         3215 g         1329 t
ORIGIN      
        1 cgcgcaggat cacacgctcg cctgccacga cggccacccg accgcgcgga gcgcgcgccc
       61 gcgcggcgtg gcgggcgggc tgcgcacgca ggaggcgtaa gccgaccaat ggccgcacag
      121 ccagcgtcag cgtcggcgtc accgaggagc gcagttcgcc ggagcccgcg aagcggacgc
      181 gcagctggcg gttgacgcgc ggcttgacgg tcgcggcgaa gctcccgtcg gcggccgtga
      241 cgggcgtcga cgacgtccgc caggtgccgt cgacgcgtgc ctgcacggtc accggctgcc
      301 cgggaacggg cgcgccgtcg cgcgtggtca gcgtgcccga gacggtcgcc tcgtcgccat
      361 agtcgatcgc cgagcggggc gcgtcgagct cggcggcgat gttcgtcccc accccggtcg
      421 gcgcgacgcc ggcgaccatc gagcgcagct cctcgagctg ggagtagagc gtcgtcccgg
      481 ggcactcggt cgcgttcgtg tcgcgatggc cgagcacgcg cggcacgcgc acggtcgtcc
      541 cggcgctgta gcggttggtg ccgccgccgg cgctctgcat cacggccgtg ccgttggtcg
      601 gatagccgtg cagcggcagc ttccagcgga tcagccgcgc catcgccgcg agcgcctcgt
      661 cgctctgcgg cacgctgctg aacgtgccga tgttcgcgat cccggtcgag taggagttga
      721 agccctccgc ctgggcgccg accaccgccg cgcctacgcc ccccgcccgc ccctcgtaga
      781 gggtcccgaa gcggtcgacg aggaagttgt agccgacgtc gttccagccg ttggtgttgc
      841 ggtggtaccg gcagatcccg aggaccacct ggggtgcctc agcgcggctg tagtcgttga
      901 tgttgacggt gtggtggacg taggcggcac ggacctctcc gtagacggcg ccggtccggg
      961 gcgggcagtc gttgccggcc cagctcgagc gcgggacgat ctccggctgg gtgtcctggg
     1021 cgcccgcgag ctcggcgctc agcaggcccg cggccgtcgt gacggcggtg ttcgcggccc
     1081 cgcgcagcgc gctcttcacg ctgtcggccg ccgtcgccgt cccgtcggtg ttgacgaagt
     1141 gcagccgcac gccgccgagc cgctccgagg agcggtactg cacccagtcc gcctcaccgg
     1201 cccagaccgg gttcgacatg ccggcggcgt gcgcctcgtc gctgcccggg tccggcccgt
     1261 cctcggcgtg tgctgtgacg ggcgcccagc gcgaccactc gccatcgtcc gtgcgcgttc
     1321 tcatcgccag cgccggctcg ctgccgtgcc cttcgacggg cgctccgtcc gaccaggtca
     1381 gcccgacgag gttgaaccgc ttcggcgcct cgatgggctc ggacaccacg ccctcggacg
     1441 gatcgggatc gccgagcaca ccgccggccg cgaccgcgaa gtcgaccggc tcgggccggt
     1501 acggcttcgt ggagagcgcg ggcgccgcca cgacgccgag tgcggcgacg agggcgatcg
     1561 ccgcaattgc cagtgcgcgt cccatccacc aatcgaacac gacccggccg catccgttgc
     1621 ggcctgcaac cgcggttgcg ctaggacgag ctaggagtgt ctaagggacg ggctcggggc
     1681 cgcgcgagcg attctcgtcc ttgaacgact cgatcgccgt gaagatgtcc tcgttgtact
     1741 tcgcgcagcc gagcagctga ccggtcccgt tcggctgcgg gtcacggttc caggcggttg
     1801 ccgcgacggc gtacttcatg ccggtcatgt ccgggacgag gaccatctga tagctgtcgt
     1861 cgtcgtagta cttcttgagc gcggcgcgct ggtccgcggg aaggttgcgc ttgaaccaga
     1921 tcaggatgcg gccgtgctcg agcgtgtgga cgagctcctt gacgtcgggc gggtcctcgt
     1981 aggcgccatc ctcggcggcc acctggtagt gccggccgga cgtcggcggc ttcgacgcgt
     2041 agttcacctt ctggctgagg tcggcgaggt gctcgcggct ctcggctggg tagctcttga
     2101 gctcgcagtc ggctgccttc gccgccgccg cgaccggcac gtcgtcgcgc ggctcggggt
     2161 agctgcctcc gtccggcagc acgtcgccgg acgccgagct gctgccgccc ccgccgccca
     2221 ggagcgcgac cgcgacgatc acggccacca ggacgagcgc gccgccgacg ccgtagccga
     2281 tcagctgctt gcggcgctgg cccgccttgg cggcggcctc gcgctgctcg cgctccttgc
     2341 gcaactgctc cttctgctcc ctccggctgg ccattcgcga cggaagacta gtgggcgcgc
     2401 gcggctcgcg ccacacctat gtgcggaagc gaccagcacc cgtcgacgaa ggtggcgatc
     2461 tcgccggtca gccgctcggg ggtgatccgc agctcgacga tcgaggacgc ctcgacgatc
     2521 gtcgcgttcg gcagctcgtg gacgagcatg tcggcgtccg agaaggggtg gatcgggtcg
     2581 cgcggatggc cgatcaccag cgtcggcggc ttgagcgtgc ggcgcacctc acgcggtggc
     2641 gcgacgcggc cgaagaacag gccctggagg accgaggccg acggcttcgg gtcctggctg
     2701 acccagtcga gcagcatctt gccgagcagc ggtccgcgac ggggcagcag ccgcgcggcc
     2761 cgactgagcg gtcttgtgat cggcgcgccg aaggtcagcg acaccaacag cggcgtgaag
     2821 ccgatcgcgg cgccgagcag cgcgttgtcg agcaccggca tctcgaccag caggcccttg
     2881 acgcgcgact ggtcgagcgc cgccgcctcg agcgcgctgt tggcgccgag cgacgtcccg
     2941 ccgagcacgg cctgctcgat gccgaggtgg tcgagcaggc cgatcgcctg gccgccgaag
     3001 gccgtcatcg agtactccca catctcctcc gggcggtcgg agtcgccgtg gccgagcagg
     3061 tcgaggcaca ggacgcggtg gccgcgggcg gcgagctcct cagccaacgg cccgtgcatc
     3121 ttgcggctga agagcagccc cggcatcagc acgaccggcc ggtcgccgct gccgtactcg
     3181 tcgtagacga gccggtggcc gtcgtggcgg aagctgcggg tgcgcggcgc catcctccgc
     3241 tgaagcttaa gcctcggccg ggcgcggctc gccggtgggg gtctcgctga gcccgaagcg
     3301 gtcgtgcagc cgccgcagcg gccgcggcgc ccaccagttc cacctgccga gcagctccat
     3361 cagcgacggc acgagcagcg cacggatgat cgtcgcgtcg agcagcacgg ccagtgcggt
     3421 gccgatcccg ttctgcttga tgaagatgat ctccgatgtc gcgaaggcgc cgatcgcgat
     3481 cgcgaacagc agcgccgcgg cggtgacgat ccggccggtc cgctcgagcc cgatcgcgac
     3541 gctgtcggtg tcggaggcgc ccttgtcgcg cgcctccttg atccgcgaga gcaggaagac
     3601 cgcgtagtcg gtcgacagcc cgaaggcgac ggcgaacagc agcacgggca tcgtctgctc
     3661 gagcgcgccc tggctcgtgt agtcgagcag cccctcgagg cggccgtcct ggaagatgaa
     3721 cacgaggatc ccgaagaccg cgctcaagtt caggacgttc atcagcagcg ccttgagcgg
     3781 cagcacgacc gagccggtca tcaggaagag gatcagcagc gtcgcgatca cgacgatcgc
     3841 gaccgcggtc ggcaggtggt cgacgaggct gctctggaag tcgatgaagt ccgcggttcc
     3901 gcccgtgacc tggaccgtca cgccgggcgg cggatcgagc ccgcggatct cgcgcaccgt
     3961 ctcctggctc gcctcggaga tgtgcggcgt ggcggagatc gcctggatca gcgtgtcgcc
     4021 gctctcgagg gcggtcggcg caaagatccg gtcgatgccc tgcaggcgcg cgatctgctc
     4081 gctgacggcg gcgatctttc ggggatcgga gtcggcgccg ctgaccagca cgtcgatcgg
     4141 cgtgtcgcgg aacggcggga aatcgctgcg cagcacgtcg tcgacctggc gggcgctctt
     4201 gtcctggggc agcacctgtg cgtcgaccga cgtgaactcg atcgaccaga acgggattcc
     4261 cagggtaagc atgaggacgg tggcggcgat cgcgatccgg cccggtatgc gctgcacgaa
     4321 gcgcgacagg cggtaccaga agcccgcgct gtccgggcgc gcgtcggcct cagcgcggcg
     4381 ctggaggaac ttcggcgcac ccgcgttgac gcggttgccg agcagcgtca ggacggccgg
     4441 caggacgatc agcgagaccg ccgccgcaag cagcgcgacg gccgctccgc cgagccccat
     4501 cgagtagagg aagcgctgcg ggaagaccag cagcgaggcg agcgccgcgg cgacggtcag
     4561 cgagctgaac agcacggttc gcccggcggt cgccatcgtc cgtcgcatcg ccgcaagtcc
     4621 cgggccgtcc ttggcgatct cctcgcggta gcgcgagacg acgaacagcg agtagtcgat
     4681 agccagcccg agccccaggc cggtcgtcag gttgagcgcg aagatcgaga tcgagccgag
     4741 ctccgacgcg atgctcaggg tcaggaaggt tccgacgatc gccagcccgc cgacgagcag
     4801 cggcaaggcc gccgcgacga ggctgcggaa gaacagcagc gacagcagga acagcagcgg
     4861 gaaggcgagc atctccgcgc gctggaggtc cgcctcgacc tggtcgttga cctgtgcctg
     4921 cgcgagcgcg ggcccaccga ccttgacccc gggggtgccc gcgaggtcgt cggcaatccg
     4981 ctcggcggca gcctgccact ccttgtcgtc ggtcggcttc agggttaccg cgaggtactg
     5041 cgcactgccg tcctcggcga cgaagtcgag ctgacgcgtg gtcccgacgc ccgccaccga
     5101 ggcgacgtcg gggtcctcct ggagctgcgc ctggatctga gcgacgcgcc gctcggtgcg
     5161 ggggctctcg accttgccgt cgatcagcac gaccacgctg gtggcgcggt gccccgcctc
     5221 gcgcagcttc tcgcgggcct cgtaggtctc gctgtccggg tcgtcggccc cataggggtg
     5281 gagcttgtcc gccacgccgc cgccgaccgc gccggcgacc gcgaagaaca ggacggccag
     5341 gacgaccacg agcttgccgc ggcggtcggc aaggcctgcg agcgcgtcga acatctcagc
     5401 tacctcccgt ggtggattcg gtctcggcca gccgccgctc caggcggtcg cactgatcga
     5461 cgaagaactg ctggtaggcg atcccggtct ccagcgtcaa cagcggcatc gcgtcacccg
     5521 tctcctcggc gagcgccgtc gccgccggct cgatgcccgc gagctcctca cgcattcgcg
     5581 tgtgcttcac gcggatctgg ttgagcagtt cgatccgctc gtcgcggtcc agcgcgtcag
     5641 cgaagaacaa ccgcagcacg ccctcgtgcc gcagctcgga gtgcagctcg cccggtcgct
     5701 tcagccactc tcgcagcgca tcctcccccg acgccgtgat cgagaacacc cgccgttgcc
     5761 gcccgttacc cgcgtcggcc tcgccctcga tcagccccgc ctgctccagc cgcctcagct
     5821 ccggatagat ctgcccgtag ctgatcgccc agaagaaccg cgtcgagacg tcggccttcg
     5881 ccttgatctc atagccgctc ttcggcccct tgtagagcat cccgaggatc acatagccgg
     5941 tcttgccaac ctccatatag cagcaagata tatcacaccg ataggtccgt caaggctcgc
     6001 ctccgcggtg gcggagacag gacccgcggg cagccccgcc tatcccgaag ccctcggatt
     6061 ctcaatcggc gtcagcgtcg cctcgatccg ctcgcgcagc ggctcgaact tcggcggcag
     6121 caccagcttc tctcccagat gcgccggatc ctcgtccgcg tcgaacccgg gccccagcgt
     6181 cgcgatctcg aacagcacgc cgctcggctc gcggaagtag atcgagcgga accagaagcg
     6241 gtcgatcacc tcggtcgcgc gtgccccgcc cgcaagcgcg cgctcgcgcc acgcctcgtg
     6301 gtcgtccatc tccgacgccc acgcgatgtg gtggacggtc ccagcgccct cgcgccccgg
     6361 ctcggacggc ggctcgtcgt agatccagag gccgccgcga ctcgccccgc gtacctcgta
     6421 ggcgcgtgtg gctcgcatgt cgccgggatg ggcggggtcc tcgtcgaccg gcccgaagcc
     6481 gagctgctcc tcgatcagcg gccggctcgc ctcagggtcc ctcgtgtacg cgcgcgccgc
     6541 gtggaagccc tgcagcgcca gctcgcgcgg gacctcgggg tggtcggcga tcagcggctg
     6601 gtcgggtacg tcggcgacca gcagctcgtg gccgagcccc tctgggtccg cgaacttcag
     6661 cgagccctcg ccgcgctcga tcgagacgcc ctcggcagcc agtcggccgg cccagaagtc
     6721 gagtgccgcc tctgaggcga cgcgccagac gatcgtgtgg accatgcccg cgccggcgcg
     6781 gccgcgcgcc gcccccggga actcgaagaa ggttatgtcg gcgccggggc tgccctgctc
     6841 gtcggcgtag aagaggtggt agaccgacgg ctggtcctgg ttgaccgtct tcttgaccag
     6901 ccgcagccca agcacgcgcg cgtagaagtc gacgttgccc gccgcgtcgc cggtgatcgc
     6961 cgttatgtga tggatgccct cgagtcgcat cccactgaat ctactcgccg cggcgagcgg
     7021 cctcagtcgc cgttgggcag cttgacgacc gtcacgaaga agctgtcgat ctggcgcacg
     7081 acctcgatga agcgctcgaa gtcgaccggc ttggtgacgt aggcgttggc gtgcagcttg
     7141 tagctgcgca gcacgtcctc ctcggcctgg gaggtggtga ggatgaccac cggaatcgtc
     7201 gcaaggtcgt cgtcgctctt gatctccgcg agcacctcgc ggccgtcctt gcgcggcagg
     7261 ttgaggtcga gcaggatcag gtcgggccgc ggcgcgccgg cgtactcgcc ctcgcgtcgc
     7321 aggaaatcga gcgcctggac gccgtcggtg accgaggtca gccggttgcg gaccttgttg
     7381 tcctcgaacg cctcgcggat catcagcgtg tcgccggggt cgtcctcgac caggagcacg
     7441 tcgatcgact tggcgttgtc cggcggtggg gaagtcatcg gctctcctcg agtgctggca
     7501 gtgtgaagcg gaaggtcgtg ccgggcccgg cgccggggtc cagccagatc cgtcccccgt
     7561 ggtgctcgac gatcttgcgg cacatcgcca ggccgattcc ggtgccagcg tactggtcct
     7621 tgccgtgcag gcgctggaag atcacgaaca cccgctcggc gtactcgggc tcgatcccga
     7681 tgccgttgtc cgagcacgtg acgacccagt cgtcgccttc gcgctcggac gcgatgcgga
     7741 cctccggcgg ggcgtcggag cggaacttga tcgcgttgcc gatcaggttc tggaagacga
     7801 gtgtcagcag cgaggcgtcg ccgcgcacca ttggcagctc gccgacctcg atcgtcgcgc
     7861 cggagtcggc gacggcggag tcgaggtcgg cgagcgcgcg gcgcaccgtc tcgccgaggt
     7921 cgaccggcgt caggtcgccc gacgtccgtc cgacgcgcga gaaggcgagg aggtcgttga
     7981 tcagctgctg catccgcttc gcgccgtcga cggcgaagtc gatgtactgg tcggcgcgat
     8041 cgtcgagctg gccgcgatag cgctgctcga gcagttggca gaagctggcg accttgcgca
     8101 gcggctcctg taggtcgtgc gacgcgacgt aggcgaactg ctcgagctcg gcgttcgagc
     8161 gctcgaggtc gcgcgcctgc tcgtcgagct gcgcgtgcgc gtcacgcagc gacgccagct
     8221 cggcgacgat ccgctggcgc atcgagtcga cgtccgcggc caggtcggcg atgtcccgtg
     8281 gcccggcgcc gcgcaccgga gcgtcgaact caccggccgc aacccgtcgg acgcgacgag
     8341 cgagcgcgcc gatcggcagc gtcacggtac ggcgcagcat gatcagcgtc gtggcgagcg
     8401 ccgccacgac gagcagggcg atcccgccga aggaggccga caggaacgta gcggcgcccg
     8461 cgagcccgtc cctgccgtcc tcgcgtgctc gcgcgaggtc tgcctcctgg cgctcgagcg
     8521 ctcgccgcag cgcctcgaac aggcgctgcc cgcgctcgac tccggcgctg ccgatcgcgc
     8581 cggccccgtc ctggcgcacg cgagcgatcg tcgggcgcgc gtagcgcgtg cgccatgcgt
     8641 gggctcgccg ctccacctcc gccaggtcgc tggagagctc gtcgagcccg gcgccgcgca
     8701 gctggacgcg cagccgccgc accgcccggc gctcctcgac gcgtccggcg cgataggggg
     8761 cgaggaacgt cgcgtcgccc gccagcacgt agccgcgcac gccggtctcc tcgctcacga
     8821 gcgcgttcgc cagccgcagc gactccaccg cggccgggtc gaggtggtcg acgaccagct
     8881 cgcgctcgtc ggagaggcgg acgatcgcga cgacgccgat gacgatgccg gtcagcgtcg
     8941 cgaggctgag caggccggtc gcgatcgcga accactggtt gacgcggaag cggttgccgc
     9001 gccggccgcc ggccggcgcg ggccgcgg
//
LOCUS       DATABV010000211        17111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2881809, whole genome shotgun sequence.
ACCESSION   DATABV010000211 DATABV010000000
VERSION     DATABV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17111
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2881809"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /gene="ilvN"
                     /locus_tag="VHF88_09720"
     CDS             <1..208
                     /gene="ilvN"
                     /locus_tag="VHF88_09720"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX2162083.1"
                     /translation="DGDSRAQIMQFAEIFRGKIVDVHKRSLTVEVTGNDDKIEAFEEM
                     VRPFGLIEMVRTGEIAVSRGRGTT"
     gene            242..1699
                     /locus_tag="VHF88_09725"
     CDS             242..1699
                     /locus_tag="VHF88_09725"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX2162084.1"
                     /translation="MTLEFGAGQVESPFGLDDESRDRFEGRVVNALEWATRGAGRTFA
                     SVTVPIEASIDVAAVVLAARRADDRWFCFEQPDRDRYAVCGLGTAAVARSEGRGRFSM
                     VAAVCRDLGAHILADDASSDPGRPPGSGPVFVGGFAFAPEGGRAPEWCSLAPAQLVLP
                     EVAFARQGGEARMTLSLEVDGSASVDELLARAEARLADLRPATMPMLDPDPAVRPRVA
                     AAAPPAHYEEAVQRAVERIRAGALAKVVLAREVRVHAPAPIDPAPILDALRGAFPACY
                     SYCVGTPELAFLGASPELLVRRDGERAQTVALAGTTRRSADPAVDDHLGEQLLRSAKD
                     RGEQQIVTERIERALDPVSLWVAAADEPVLVKVQNVQHLATPIRAQLADPIPVLELAG
                     RLHPTPAVGGEPNAAVQPLIPALEGLDRGWYAGAVGWTDLAEDGELCVALRCALLRDD
                     LAHLYAGCGIVADSIPADELAETEVKLQALLPLLA"
     gene            complement(1822..3567)
                     /gene="menD"
                     /locus_tag="VHF88_09730"
     CDS             complement(1822..3567)
                     /gene="menD"
                     /locus_tag="VHF88_09730"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX2162085.1"
                     /translation="MIPINRTFAPVQALVDELARCGMRQAVTSPGSRNAPITLTLAAD
                     ERIDAVSAIDERAAGFMALGMAKASGRPVAVTCTSGTAAANLLPAIVEAREARVPLIV
                     LTADRPPELRDVGAGQAIDQIKLFGSAAKWFVEVGNGEPGPEWATHVRALACRAWWTA
                     GGGRPGPVHLNLPLREPLAPVAEELEAGDWQGREDGRAWVEAIEGAAVPNDEDVQALA
                     ERMAATARGVIVCGGMATASDDSATVSLVEAVTRLGREAGWPLLAEPTSGLRCGEHDR
                     SQVVAHYDVLLRDEGFADAQRADLVLRIGDMPTSKSLRAWVGRAEQVVIDPQAVWNEP
                     TRTAETILAADLTLTCEALAAAIEDVGTSADGGWVGRWRAADGVVASAFAEAPETFEA
                     KAYAGIEAALPDDALVWVSSSMPIRYVESYFPASPKRLRFLSNRGANGIDGVVASAAG
                     AARATGRPTFVLIGEVALVHDVGGVLAARRAGIDLTIVCVNNGGGGIFDFLPVAELSD
                     KVAYERHIATPAGVDLASLAALAGMPHRLVTTKAELASAIAAGPRLIELVADRGDSVE
                     RSRALTATVADRLGD"
     gene            3574..4326
                     /locus_tag="VHF88_09735"
     CDS             3574..4326
                     /locus_tag="VHF88_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2162086.1"
                     /translation="MSKERNGPSVIFVPGFMQPAEAWADVAERLPQRYPSLLLRHSEH
                     EREGRIAEIAAAAEAVDGEVVLCGYSLGGRLALNAAVRRPDLYAAVVVLGAAAGIDEP
                     AARQARREADEKLAAWMETQPIEKIVEVWERQPLFGDQSERLIEAQRPGRLSQDPRSL
                     AVLLRTAGQGAMEPIWGRLHTLDLPILALAGSRDERYRRATRRIAAEAPQGYAAVIEH
                     AGHAAHLQRPGEFADALLAFLDERVRSVEPVD"
     gene            complement(4306..5319)
                     /locus_tag="VHF88_09740"
     CDS             complement(4306..5319)
                     /locus_tag="VHF88_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX2162087.1"
                     /translation="MKRSLLRLRIPLRVPFVTANGVLAERELVVVRLEDDQGTPGWGE
                     AAPLEPYDGVSVDTVWAALADGPPPRDAPRHARTAWELSELDLQARRRGRPLGDHGAD
                     AIPVNMTLPAGPPGEVAAAAARGLREGYSCFKLKVGLPDDDVRVAAVREALGTWPALR
                     IDANGAWTAAQAVERIRALAVHDLELVEQPCATLEELAEVRAKVETPIAADEPIRGPE
                     DVRRAAELGACDAVNLKLAGSGGFLPARDALRAAREAGMSAWLSSTLDGPWTIAAALQ
                     LASQEHVTLACGLATLDVFDAPIARALPGPRQGLMHVPEGPGLGVDLAGEVLDPLLVD
                     RLD"
     gene            complement(5316..6230)
                     /locus_tag="VHF88_09745"
     CDS             complement(5316..6230)
                     /locus_tag="VHF88_09745"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX2162088.1"
                     /translation="MTPVADAPHAAGGALRLWALAARPRTLPAAVAPVLVGTALAIVD
                     DEFKPLAFVAALIGSIFIQIGTNLSNDYSDARRGADTEDRLGPVRVTAGGLMPPRRVL
                     VGTYVAFGIAVVAGIYLAAITGPELLLVGVASIAAGVLYTGGPRPYGYEGLGELFVFL
                     FFGVVAVVGSYYVQAEELPWEAFALSVPVGLLAAAILVVNNVRDADTDRRAGKRTLAV
                     RLGRARARGLFGAMIVVSYLTVVAIPLAGGLSRWVALPLLTLPLVPSLWRAVAYRSDG
                     PSLNAALADTGRLLGLFSLLLAAGILLS"
     gene            complement(6227..7129)
                     /gene="menB"
                     /locus_tag="VHF88_09750"
     CDS             complement(6227..7129)
                     /gene="menB"
                     /locus_tag="VHF88_09750"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX2162089.1"
                     /translation="MAATDETRAGGDWAAEQARRSIAAGAEWQRAGDYEDIRYETADG
                     IAKVTIDRPEVRNAFRPQTIVEISDALERAREDTAVGVIVLTGEGPLAFCSGGDQRVR
                     GDSGYMTDPAGGKAAVGRFHVTDLHVQIRRLPKPVVAMIAGYAVGGGHVLHIVCDLSI
                     AADNARFGQTGPKVGSFDGGFGASLLAAQIGQKKAKEIWFLCRQYDAREALEMGLVNS
                     VVPLEQLEVETVRWCREMLALSPFALRLLKASFNASEDGMAGIQQLAHDANLLFYGSE
                     EAQEGRDAYREKRKPDFGKFPRRP"
     gene            7276..8628
                     /locus_tag="VHF88_09755"
     CDS             7276..8628
                     /locus_tag="VHF88_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2162090.1"
                     /translation="MHDWVREAARRKPDRGAVEASDGVLSYAELDERADAVARRLAAL
                     GVGEGGRVATTVPPSTVFALLLHAAPRIGAALVPLNTRLTVDQQRAQAAAVGADVVVN
                     GPLDGMEAAIEPRRELDASAVHTVLFTSGTEGEPKPVELTAGNLDASAAASAAAFGVE
                     SGDRWLSPLPLFHVAGLSILTRCARNATTAVLRDRFDPTAVAGALAEGGVSLISLVPT
                     QLSRLRHSGLTEAPGLRALLLGGGPIPPDLVDWARSAGLPVRCTYGMTETASQVAVTE
                     PWESAATPLPGVELEVAPDGEILVRGPMVAPGAVRPDGWLHTGDLGSVDRRGRLHVVG
                     RLKELIVSGGEKVAPAAVEAVLAAHPAVADAGVAGTPDPDWGEAVTAFVVERHPVSDY
                     DLLGFCRERLAGYQVPKRVVRVAELPRNAGGKLIRARLPVEASQAPPGGAGGASPPIK
                     "
     gene            8640..9629
                     /gene="ilvC"
                     /locus_tag="VHF88_09760"
     CDS             8640..9629
                     /gene="ilvC"
                     /locus_tag="VHF88_09760"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX2162091.1"
                     /translation="MIYDDDADLSKLDGKTVAILGYGSQGHAHALNLHDSGVNVVVGL
                     REDSSSVEKAKSDGLEVASIADAASRGDVVMVLLPDEKQGKLWEEEIRDGIAPGNLLL
                     FAHGFSIHFDQIDPGPGVDVGMVAPKGPGHLVRRQFTEGAGVPGLMAIHQDASGNARD
                     LVLAYAKGIGCTRGGVIETSFKEETETDLFGEQAVLCGGVSELVQAGYETLVDAGYDP
                     RLAYFECLHELKLIVDLMYEKGITGMRYSISNTAEYGDLTRGKRVITDETRAAMKEIL
                     GEIQSGQFAREWIAENNAGQESFKRMREEQAGKQIEREGRELRSKMSWIDTEF"
     gene            9637..10761
                     /gene="serC"
                     /locus_tag="VHF88_09765"
     CDS             9637..10761
                     /gene="serC"
                     /locus_tag="VHF88_09765"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR01366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX2162092.1"
                     /translation="MRIPDHLKPADGRFGSGPTKVRPEQLNRLAGSGAAMMGTSHRQK
                     PVKAVVGQIRSGLGELFSLPDGYEVALGNGGTTAFWDAATAGLVRSRALHLTYGEFSQ
                     KFATATAGAPFLEDPIIVSADPGDAPAPASDPAADVIGWAHNETSTGVMVPVQRPADS
                     GDALVLIDATSGAGGLPLDASQADVYYFAPQKGFASDGGLWLALLSPAAIERIEELGA
                     ATDRWRPEFLSLTTALDNSRKDQTYNTPALATLLLLADQIEWMLSGGGLDWCVQRTSA
                     SSRHLYEWAEARDYATPFVVDTAKRSLVVGTVDFDDSIDAAAIAATLRANGIVDVEPY
                     RKLGRNQLRVGMFPAIEPGDVEALTACIDWTIENVGEVTR"
     gene            10758..12356
                     /gene="serA"
                     /locus_tag="VHF88_09770"
     CDS             10758..12356
                     /gene="serA"
                     /locus_tag="VHF88_09770"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX2162093.1"
                     /translation="MKVLVKEKIGESGVQLLRDAGLEVELGIDWDDEELERRIGEFDG
                     ILIRSATKLTADLIEKGDNLKAIGRAGVGVDNVDVPAATRRGIVVANAPQSNVVTAAE
                     HTMALLLALARNIPQAHASLVGGAWDRSKYSGTELYEKTLGIVGFGRIGQLVAQRARG
                     FGMHVIAFDTFISEERFRELGVERADSADAVYAVADFLTIHLPKTPETEGWLNAEVFA
                     KCKDGVRILNVARGPLLVDSDLQAALDSGKVGGAALDVFQTEPITEHPLFGHPSVVVT
                     PHLGASTAEATDRAGYQAAEQVIAALTGGTVTNAVNAPAVAGEDLEALGPFLPLAAHL
                     GRIAATLTEGTSVDGIEVEVLGRLAERDTRLVTVQVIKGVLAGHTEEEVNDVNAPTLA
                     EERGIAVSSTTSERARDFTDLVRVTVVSGSDRARVVGTVLGNRDRPHLLEAWGSRFNV
                     QLEPQLAIFRYRDQPGMIGRVGTVLGEAGVNIVSAAVGRQPDDAATGEAVMVVTADAE
                     VPQDVVNAIVASDGFVAGRTVSLV"
     gene            12520..14109
                     /locus_tag="VHF88_09775"
     CDS             12520..14109
                     /locus_tag="VHF88_09775"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX2162094.1"
                     /translation="MATSETRDEASRVRIFDTTLRDGEQSPGISLNTQEKLEIAHQLG
                     RLGVDIIEAGFPIASPGDFEAVQAIAREVHGPIICGLARTGVQDIDAAWNAVKDSERP
                     RIHTFISTSDIHIQHQLQTTREDVKGQARAAVAHAKGYCDDVEFSPMDATRADLEFTA
                     EVLQIALDEGATTINVPDTVGYSMPEEYASMWRELYRLVPGLADVVTSVHCHDDLGLA
                     VANSFAGVQAGCRQVECAINGLGERAGNASLEEIVMLLAVREAEHGLWTGVETTEIAR
                     TSRLVSRLTGYQVQPNKAIVGRNAFAHESGIHQDGVLKERSTYEIMDATTIGLESNSI
                     VLGKHSGRHALRKALEELGFTVDGAALNTAFKRFKEIADRKSNVTALDLEAIVSDEMR
                     QSESAFELESFDVEAGSDRSPFARVRVRMPGGAVADGEFTGDGPVDAFFSALNVATGR
                     EARLKEYHVSAVTAGRDALAEVTVLLELDGVTAQGQGVSPDTMEASGRAYLRAITNAL
                     ADSPVAEAEHHIDAAAKSATP"
     gene            14246..15250
                     /locus_tag="VHF88_09780"
     CDS             14246..15250
                     /locus_tag="VHF88_09780"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX2162095.1"
                     /translation="MDFTWVDGERLIRFGAGVLGEAPGLLAQRGFEGYALLTTERAVA
                     GVPVLAEGASTVLHVPVGSVPDIAAAVLPDVGDRPVVALGGGRVVDAAKAVAGVERLP
                     CAAVPTTLAGSPMTPFHRLPAGTRGGQLVRPSLVVWDPELIGTLPREQLVATGMNALA
                     HAFESLYVPHANPVAEMAALRAASLFGEQLQRESPNVVDVALAALLAGYAVGTTGFAI
                     HHALCQTTVRVAGLPHAQVNALVLPHTAGLMASRAPAAVGRFATALGDVAGDPLAAPN
                     RIGELAAGIGVGGLAELGLDSDSISAIAHAAIGHPAIGNTPGGTPAEVDLAGILTAAL
                     "
     gene            complement(15276..16178)
                     /locus_tag="VHF88_09785"
     CDS             complement(15276..16178)
                     /locus_tag="VHF88_09785"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX2162096.1"
                     /translation="MTRLQRNANIAAVIVPFVAVLAAIPLLWNRLIGWTDVGIMVGVY
                     LATAFGITIGFHRLLTHRAFETHRPLKYLFAILGSMAVQGPVIGWVADHRKHHAHTDE
                     EGDPHSPHVGEGSGLRGLWHAHVGWLFEHNGVADAKRYAPDLLEDPGMRFISRHFLWW
                     VILGLAIPFGLGFAIGGTLEAAALALFWGGLVRVFFVHHVTWSINSICHFFGERRFQT
                     DDRSTNVAWLALPSLGEAWHHNHHAFPRAAVQGMRWWEIDISGLTIRALEKLGLAWNV
                     ITISPERQQQRLAEGPARQVRQAA"
     gene            16263..>17111
                     /locus_tag="VHF88_09790"
     CDS             16263..>17111
                     /locus_tag="VHF88_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162097.1"
                     /translation="MTVLDQRPWVALPPAAAEALRPELPAIAEEIIGAISDGVPEYAL
                     PLEGRFGEGLRMGVERALQRFLDLSGSDDGGPDPGRQVYVDLGRGELRAGRPLEALLA
                     AYRLGARVAWRRLAAAGERAGLEPRTLYLLAESIFAYIDELSGESVEGYAMEQAAVAG
                     AQQRRRRELAALLVQDPPADATAIEAAALAAGWPLPRTVAAVYVEAGAGEAPADPDRL
                     SNRLGAGTIAAELAPGACALVPDPDAPGRRAQLAAAFRDGVAALGPSVPWREGHVSAG
                     RARAALG"
BASE COUNT         2423 a         6171 c         6182 g         2335 t
ORIGIN      
        1 cgacggcgac agccgcgccc agatcatgca gttcgccgag atcttccgcg gcaagatcgt
       61 cgacgtccac aagcgctcgc tgaccgtcga ggtcaccggc aacgacgaca agatcgaggc
      121 cttcgaggag atggtccgcc cgttcgggct gatcgagatg gtccgcacgg gcgagatcgc
      181 cgtctcgcgg gggagaggaa cgacctagcc ggtcgccggc gtcgcgccta agcttcagcg
      241 cgtgacgctc gagttcggcg caggccaggt cgagagcccg ttcgggctcg acgacgagtc
      301 tcgtgaccgc ttcgaggggc gggtggtcaa cgcgctcgag tgggcgacgc gcggggcggg
      361 gcggacgttc gcctcggtca cggtgccgat cgaggcgtcg atcgacgtcg cggcggtggt
      421 gctcgcggcg cgccgtgccg atgaccgctg gttctgcttc gagcagccgg accgcgaccg
      481 ctatgcggtc tgcggtctgg ggacggcggc cgtggcgcgc tcggagggtc ggggtcgctt
      541 ctccatggtc gccgcggtct gtcgcgacct cggggcgcac atcctcgccg acgacgcgtc
      601 gtccgatccc ggccgcccgc cgggatccgg accggtcttc gtcggggggt tcgcgttcgc
      661 gcccgagggc ggacgcgcgc ccgagtggtg ctcgctcgcc ccggcgcagc tcgtcctgcc
      721 ggaggtcgcg ttcgcccgcc agggcggcga ggcgcggatg acgctctcgc tcgaggtcga
      781 cggaagcgcc tcggtcgacg agctgctcgc ccgcgctgag gcccgcctcg cggacctgcg
      841 cccggcgacg atgccgatgc tcgaccccga tcctgccgtg cgtccgcgcg tcgcggccgc
      901 cgcgccgccg gctcactacg aggaagccgt ccaacgggcg gtcgagcgga tccgcgccgg
      961 cgccctcgcg aaggtcgtgc tggcacgcga ggtgcgcgtc cacgcgcccg ccccgatcga
     1021 tcccgccccg atcctcgacg cgctgcgtgg cgcctttcca gcctgctact cctactgcgt
     1081 cggcacgccg gagctggctt tcctcggcgc gagccccgag ctgctcgtcc gccgcgacgg
     1141 cgaacgagcg cagacggtgg ccctggccgg caccacccgt cgcagcgccg accccgccgt
     1201 cgacgaccac ctcggcgagc agctgctgcg cagcgcgaag gaccgcggcg agcagcagat
     1261 cgtcaccgag cggatcgagc gggcgctcga tcccgtcagc ctctgggtcg cggccgctga
     1321 cgaaccggtg ctcgtcaagg tccagaacgt ccagcacctc gccacgccga tccgcgccca
     1381 gctcgccgac ccgatccctg tgctcgagct ggccggccgg ctacacccga cccctgccgt
     1441 cggcggcgag cccaacgccg ccgtccagcc gctgatccca gcgctcgagg gcctcgaccg
     1501 cggctggtac gccggcgccg tcggctggac cgacctcgcg gaggacggcg agctctgcgt
     1561 cgccctccgc tgcgccctgc tgcgcgacga cctcgcccac ctctacgccg gctgcggcat
     1621 cgtcgccgac tccatccccg ccgacgagct cgccgagacc gaggtcaagc tccaggccct
     1681 attgccgctc ctcgcctgat cgagcgaagc attgttggcc ctatagcccg ctcaatcctt
     1741 cggtcgaccg tcggacgcgc cgtcgccgac gactcgatga ggggtttgct cgccctggcc
     1801 ggcacaaacc cacatcggag gtcagtcccc caggcggtcg gccaccgtcg ccgtcaaggc
     1861 gcgactgcgt tcgacgctgt cgccgcggtc ggcgaccaac tcgatcagac gcgggccggc
     1921 ggcgatcgcc gaggcaagct cggccttcgt ggtgacgagc cggtgcggca tgccagcgag
     1981 tgcagcgaga gacgccagat cgacacccgc gggcgttgcg atgtgtcgct cgtatgccac
     2041 cttgtccgag agctcggcca ccggcaggaa gtcgaagatc ccgccgccgc cgttgttcac
     2101 gcacacgatc gtcaggtcga tgcccgcccg tctagctgcg agcacgccgc cgacgtcatg
     2161 caccagggca acctcaccga tcaagacgaa cgtcggccgt ccggtggcac gcgctgcgcc
     2221 cgcggctgaa gcgacgacgc cgtcgatccc gttcgcaccg cgattcgaca ggaagcgcag
     2281 ccgcttcggc gacgcgggga agtaggactc gacgtagcgg atcggcatcg acgagctcac
     2341 ccagacgagc gcatcgtccg gcagcgccgc ctcgatcccc gcataagcct tggcctcgaa
     2401 cgtctcgggc gcctcggcga acgcactggc caccaccccg tcagcagccc gccacctccc
     2461 tacccagccg ccgtcagcgc tagtgccaac gtcctcgatc gcggcagcca aggcctcgca
     2521 ggtcagcgtc agatccgctg ccagaatcgt ctccgccgtc cgcgtcggct cgttccagac
     2581 cgcctgcgga tcgatcacca cctgctcggc tcggccgacc cacgctcgca acgacttcga
     2641 cgtcggcatg tcgccgatcc ggagcacgag gtcggcacgc tgagcgtcgg cgaagccctc
     2701 gtcacgcagc agcacgtcgt agtgcgccac gacctgggag cggtcgtgct cgccgcagcg
     2761 cagtcccgac gtcggctcgg ccagcagcgg ccagccggcc tcgcggccca gtcgagtgac
     2821 ggcctcgaca aggctcacgg ttgccgagtc gtcggacgcc gtcgccatgc ccccacacac
     2881 gatcacccca cgcgcagtcg ccgccatccg ctcggccagc gcctgtacgt cctcgtcgtt
     2941 cggcaccgcc gcgccctcga tcgcctccac ccacgcccgc ccgtcctcac gcccctgcca
     3001 gtcaccggcc tccagttcct ccgccaccgg cgccagcggc tcacgcagcg gcaagttcag
     3061 atgcaccggc cccggccgtc caccgccagc cgtccaccac gcgcgacagg ccagcgcccg
     3121 cacatgcgtc gcccactcgg gccctggctc accgttgccg acctcgacga accacttcgc
     3181 ggccgagccg aacagcttga tctggtcgat cgcctggccg gcgccgacgt cgcgcagctc
     3241 cggcggccgg tcggcggtca gcacgatcag cggcacgcgc gcctcgcgcg cctcgacgat
     3301 ggctggcagc aggttggccg ccgccgtccc cgaggtgcag gtcacggcga ccggccggcc
     3361 gctcgccttt gccattccga gcgccatgaa cccggcggcg cgctcgtcga ttgccgacac
     3421 cgcatcgatc cgctcgtccg ccgccagggt cagcgtgatc ggcgcgttgc gcgagccggg
     3481 cgaggtcacc gcctgccgca tcccacagcg cgccagctcg tcgacgagcg cctgtacagg
     3541 cgcaaaggtg cggttgatcg ggatcatgtg cctgtgagca aggagcgcaa cggcccgtcg
     3601 gtgatcttcg tcccgggctt catgcagccc gccgaggcgt gggcggatgt cgccgagcgg
     3661 ctgccgcaac gctacccgag cctcctgctg cgccactccg agcacgagcg cgaggggcgg
     3721 atcgcggaga tcgccgccgc tgccgaggcc gtcgacggcg aggtcgtcct ctgcggctac
     3781 tcgctcggcg gacggctcgc cctcaacgcc gcggtgcgtc ggcccgacct ctacgccgcg
     3841 gtcgtcgtgc tcggcgcggc cgccgggatc gacgagccgg cggcgcgcca ggcgcgccgc
     3901 gaggccgacg agaagctcgc cgcatggatg gagacgcagc cgatcgagaa gatcgtcgag
     3961 gtctgggagc gccagccgct gttcggcgac cagtccgagc gcctgatcga ggcccagcgg
     4021 ccaggccgcc tctcccagga tccgcgttcc ctggccgtgc tgctgcgaac cgcgggtcag
     4081 ggggcgatgg agccgatctg ggggcggctg cacacgctgg acctgccgat cctcgcgctc
     4141 gccggatcgc gcgacgagcg ctaccgccgc gctacgcgga ggattgccgc cgaggcgccg
     4201 cagggatatg ccgcggtgat cgagcacgcc ggtcacgccg cccacctgca gcggccgggc
     4261 gagttcgccg acgcgctgct cgccttcctc gacgagcgcg tgcgctcagt cgagccggtc
     4321 gactagcagc gggtcgagca cctcgccggc gaggtcgaca ccgaggcccg gaccctccgg
     4381 cacgtgcatc aggccctgcc gcgggcctgg cagggcacgc gcgatcggag cgtcgaagac
     4441 gtccagcgtc gcgaggccac aggcgagcgt gacgtgttcc tgacttgcca gctgcagcgc
     4501 ggcggcgatc gtccacggcc cgtcgagcgt gctcgacagc caggcggaca tgcccgcctc
     4561 gcgcgcggcg cgcagcgcgt cgcgggcggg caggaagccg ccgctgccgg cgagcttgag
     4621 gttgacggcg tcgcaggcgc ccagctcggc agcccgccgc acgtcctcgg gaccgcggat
     4681 cggctcgtcg gcggcgatcg gcgtctcgac cttggcccgt acctcggcca gctcctcgag
     4741 cgtggcgcac ggctgctcga cgagctcgag gtcgtggacg gcgagggcgc ggatccgctc
     4801 caccgcctgc gccgcagtcc aggcaccgtt ggcgtcgatg cgcagcgcgg gccaggtccc
     4861 gagcgcctcg cgcaccgccg cgaccctgac gtcgtcgtcg ggcagcccga ccttcagctt
     4921 gaagcaggag tagccctcgc gcaggccgcg cgcggccgcc gcggccactt cgccgggggg
     4981 gccggccgga agcgtcatgt tgaccgggat agcgtcggcc ccgtggtcgc cgagcgggcg
     5041 gccgcgccgg cgcgcctgga ggtcgagctc ggagagctcc caggcggtgc gcgcgtggcg
     5101 cggcgcgtca cgcggaggcg ggccgtcggc gagcgcagcc cagaccgtgt cgacgctgac
     5161 cccgtcgtag ggctcgagcg gcgcggcctc gccccagccg ggcgtgccct ggtcgtcctc
     5221 cagccgcacg acgaccagct cgcgctcggc aaggacgccg ttcgcggtca cgaacggcac
     5281 gcgcagcggg attcgcaggc gcagcagcga gcgcttcacg acaacaggat gcccgccgcc
     5341 agcagcagcg agaagaggcc gagcagtcgc ccggtgtcgg cgagcgccgc gttcagcgac
     5401 gggccgtcgc tgcggtaggc gaccgctcgc cagagcgacg ggaccagcgg cagcgtgagc
     5461 agcggcagcg cgacccagcg cgagagcccg ccggcgagcg ggatcgccac caccgtcagg
     5521 tacgagacga cgatcatcgc cccgaacagc ccgcgggcgc gagcgcggcc caaccggacc
     5581 gcgagcgtcc gcttgccggc gcggcggtcg gtgtcggcgt cgcggacgtt gttgacgacg
     5641 aggatcgccg cggcgagcag cccgaccggc accgagagcg cgaacgcctc ccacggcagc
     5701 tcctcggcct ggacgtagta gctgccgacg accgcgacga ccccgaagaa gaggaagacg
     5761 aacagctcgc cgagtccctc gtagccgtag ggccgcggtc cgccggtgta gagcacgccg
     5821 gccgcgatcg acgccacgcc gaccagcagc agctccggac cggtgatcgc cgccaggtag
     5881 atgcccgcga ccacggcgat cccgaacgcg acgtaggtcc cgaccagcac gcgccgcggt
     5941 ggcatcaggc cgcctgcggt cacgcgcacc ggcccgaggc ggtcctcggt gtcggcgccg
     6001 cggcgcgcgt cggagtagtc gttcgagagg ttcgtcccga tctggatgaa gatgctgccg
     6061 atcagcgccg ctacgaacgc gagcggcttg aactcgtcgt cgacgatcgc cagcgcggtg
     6121 ccgacgagga ccggcgccac ggccgcgggc agggtccgcg gccgcgcggc cagcgcccac
     6181 aggcgcagcg cgccgccggc ggcgtgggga gcgtcggcga ccggggtcac ggccggcgcg
     6241 ggaacttgcc gaagtccggc ttccgcttct cgcggtaggc gtcgcggccc tcctgggcct
     6301 cctcgctgcc gtagaagagg aggttggcgt cgtgggcgag ctgctggatg ccggccatcc
     6361 cgtcctcgga cgcgttgaag ctcgccttca gcaggcgtag ggcgaaaggc gacagcgcga
     6421 gcatctcgcg acaccagcgg acggtctcga cctccagctg ctcgagcggc acgacgctgt
     6481 tgaccaggcc catctcgagc gcctcgcggg cgtcgtactg gcggcagagg aaccagatct
     6541 cctttgcctt cttctgcccg atctgcgcgg cgagcaggct ggcgccgaag ccgccgtcga
     6601 aggagccgac ctttgggccc gtctggccga agcgcgcgtt gtcggcggcg atcgaaaggt
     6661 cgcagacgat gtgcaggaca tggccgccgc cgaccgcgta gcccgcgatc atcgccacca
     6721 ccggcttcgg tagccggcgg atctggacgt gcaggtcggt gacgtggaag cggccgaccg
     6781 ccgccttgcc gcccgcgggg tcggtcatgt agccggagtc gccgcgcacc cgctggtcgc
     6841 cgccggagca gaacgccagc ggtccctcgc cggtcagcac gatcaccccg accgcggtgt
     6901 cctcgcgcgc ccgctcgagc gcgtcggaga tctcgacgat cgtctgcggc cggaacgcgt
     6961 tgcgcacctc gggacggtcg atcgtgacct tcgcgatccc gtccgccgtc tcgtagcgga
     7021 tgtcctcgta gtcgccggcg cgctgccact cggcgcccgc ggcgatcgat cgcctcgcct
     7081 gctcggccgc ccagtcgccg ccggcccggg tctcgtcggt cgcggccatc gccgagaggt
     7141 tagaccgccg tcccgccttg tggcgtcgac caccgcctcg gtggccggcg ccgcaagagc
     7201 ggcgccgcga ccgaggattg cggcgctgtc cacgacatcg gtggctggct ccacaaggca
     7261 cgtacgcttc gcccgatgca cgactgggtc cgcgaagcgg cgcgcaggaa accggaccgc
     7321 ggggccgtcg aggcttccga cggcgtcctg agctacgccg agctcgacga gcgcgccgat
     7381 gcggttgcgc gccggctggc cgcgctcggc gtcggggagg gggggagggt cgcgacgacg
     7441 gtgccgccgt cgacggtctt cgcgctcctg ctgcatgccg cgccgcggat cggcgcggcg
     7501 ctggtgccgc tcaacacccg actgaccgtc gaccaacagc gtgcccaggc cgcggccgtc
     7561 ggcgccgacg tcgtggtcaa cggtcccctg gacgggatgg aggccgccat cgagccgcgg
     7621 cgcgaactcg atgcctccgc ggtccacacc gtcctcttca cgtccggcac cgagggcgag
     7681 cccaagccgg tggagctgac ggccggcaac ctcgacgctt ccgccgcggc gtcggcggca
     7741 gccttcgggg tcgagagcgg cgaccgctgg ctgtcgccgt tgccgctgtt ccacgtcgcg
     7801 ggcctcagca tcctcacgcg ctgcgcccgc aacgccacga cggcggtgct tcgcgaccgc
     7861 ttcgacccca ccgcggttgc cggtgcgctc gccgaaggcg gggtctcgct gatctcgctc
     7921 gtcccgacgc agctgagccg cctgcgccac tccggtctca ccgaggcgcc cggcctgcgc
     7981 gcactgctgc tcggcggcgg tccgatccct ccggacctcg tcgactgggc acgcagcgcc
     8041 ggcctgccgg tccgctgcac ctacggcatg accgagaccg cgtcgcaggt cgccgtcacc
     8101 gagccgtggg agtccgccgc cactccgctg ccgggcgtcg agctggaggt cgcgcccgac
     8161 ggcgagatcc tggtccgcgg cccgatggtg gcgcccggcg cggtccgtcc cgacggctgg
     8221 ctgcacaccg gcgacctcgg cagcgtcgac cgccgcggcc ggctccacgt cgtcggacgg
     8281 ctcaaggagc tgatcgtcag cgggggcgag aaggtcgctc cggccgccgt cgaggccgtc
     8341 ctcgccgccc atcccgccgt cgccgacgca ggcgtcgctg gcacgcccga ccccgactgg
     8401 ggcgaagccg tcacggcctt cgtcgtggag cgccacccgg tctccgacta cgacctgctc
     8461 ggcttctgcc gcgagcgcct cgccggctac caggtcccca agcgcgtcgt ccgcgtcgcc
     8521 gagctgccgc gcaatgctgg gggcaagctg atacgcgcgc gccttcccgt cgaagccagc
     8581 caggcgccgc cggggggcgc ggggggcgcg agccccccga tcaaataaag tcgtcggccg
     8641 tgatctacga cgacgacgca gacctctcga agctcgacgg caagaccgtc gccatcctcg
     8701 gctacggctc gcagggccac gcccatgcgc tcaacctgca cgactccggc gtgaacgtcg
     8761 tagtcggcct gcgcgaggac tcctcgtcgg tcgagaaggc gaagtccgac ggtctcgagg
     8821 tcgcctcgat cgccgacgcc gccagtcgcg gcgacgtcgt gatggtcctg ctgcccgacg
     8881 agaagcaggg caagctctgg gaggaggaga tcagggacgg gatcgcgccc ggcaacctgc
     8941 tgctcttcgc gcacggcttc tcgatccact tcgaccagat cgaccccggc cccggcgtcg
     9001 acgtcggcat ggtcgccccc aagggccccg gccacctcgt gcggcgccag ttcaccgagg
     9061 gcgccggcgt cccggggctg atggcgatcc accaggacgc cagcggcaac gcgcgcgacc
     9121 tcgtgctcgc ctacgccaag ggcatcggct gcacgcgcgg cggcgtcatc gagacatcgt
     9181 tcaaggagga gaccgagacc gacctcttcg gcgagcaggc cgtgctctgc ggcggcgtct
     9241 cggagctcgt ccaggccggc tacgagacgc tcgtcgacgc cggctacgac ccgcggctgg
     9301 cctacttcga gtgcctccac gagctcaagc tgatcgtcga cctgatgtac gagaagggca
     9361 tcaccggaat gcgctactcg atctccaaca ccgccgagta cggcgacctg acccgcggca
     9421 agcgcgtgat caccgatgag acgcgcgctg cgatgaagga gatcctcggc gagatccagt
     9481 cgggccagtt cgcgcgcgag tggatcgccg agaacaacgc cggccaggag agcttcaagc
     9541 ggatgcgcga ggagcaggcc ggcaagcaga tcgagcgcga gggccgcgag ctgcgctcga
     9601 agatgtcctg gatcgacacc gagttctagg cgccgggtgc ggattcccga ccacctcaag
     9661 cccgccgacg ggcgcttcgg cagcggcccg accaaggtcc gccccgagca gctgaaccgg
     9721 ctcgccggca gcggcgcggc gatgatgggc acctcgcacc gccagaagcc ggtcaaggcg
     9781 gtcgtcggcc agatccgctc ggggctcggc gagctcttct cgctgcccga cggctacgag
     9841 gtcgcactcg gcaacggcgg cacgacggcg ttctgggacg cggccaccgc cgggctcgtc
     9901 cgcagtcgcg cgctgcacct gacctacggc gagttctcgc agaagttcgc caccgcgacc
     9961 gccggagcgc cgttcctcga ggacccgatc atcgtctccg ccgacccggg cgacgcgccc
    10021 gcaccggcgt ccgacccggc cgcggacgta atcggctggg cgcacaacga gacgtcgacc
    10081 ggcgtgatgg tcccggtgca gcggcccgcg gactccggcg acgcgctcgt cctgatcgac
    10141 gccacctctg gcgccggcgg cctgccgctc gacgccagtc aggccgacgt ctactacttc
    10201 gccccgcaga agggcttcgc gtccgacggc ggcctctggc tggcgctgct gagccccgcg
    10261 gcgatcgagc ggatcgagga gctcggcgcg gctaccgacc gctggcggcc ggagttcctc
    10321 tcgctgacga cggcgctcga caactcgcgc aaggaccaga cctacaacac cccggcgctg
    10381 gcgacgctgc tgctgctcgc cgaccagatc gagtggatgc tctcgggcgg cggcctcgac
    10441 tggtgcgtcc agcggacgag cgcctcgtcg cgccacctct acgagtgggc cgaggcgcgc
    10501 gactacgcga cgccgttcgt ggtcgacacc gcgaagcgct cgctggtcgt cggcacggtc
    10561 gacttcgatg actcgatcga cgccgcggcg atcgccgcga ccctgcgcgc caacggcatc
    10621 gtcgacgtcg agccctaccg caagctgggc cgcaaccagc tgcgcgtcgg gatgttcccg
    10681 gcgatcgagc cgggcgacgt cgaggcgctg accgcctgca tcgactggac gatcgagaac
    10741 gtgggggagg tcacccgatg aaggtgctcg tcaaggagaa gatcggcgag tcgggcgtcc
    10801 agctgctgcg cgacgccggg ctcgaggtcg agctcggcat cgactgggac gacgaggagc
    10861 tcgagcgccg gatcggcgag ttcgacggga tcctgatccg ctcggcgacc aagctcacag
    10921 ccgacctgat cgagaagggc gacaacctca aggcgatcgg tcgcgccggc gtaggcgtcg
    10981 acaacgtcga cgtgccggcc gccacccgcc gcggcatcgt cgtcgccaac gcgccgcagt
    11041 ccaacgtcgt cacggccgcc gagcacacga tggcgctgct gctcgcgctc gcgcgcaaca
    11101 tcccgcaggc ccacgcgtcg ctcgtcggcg gcgcctggga ccgctcgaag tacagcggca
    11161 ccgagctcta cgagaagacg ctcgggatcg tcggcttcgg ccgcatcggc cagctcgtcg
    11221 cccagcgcgc gcgcggcttc gggatgcatg tgatcgcctt cgacacgttc atctccgagg
    11281 agcgcttccg cgagctcggc gtcgagcgcg ccgacagcgc cgacgccgtc tacgccgtcg
    11341 cggacttcct cacgatccac ctccctaaga cccccgagac cgagggctgg ctcaacgccg
    11401 aggtcttcgc gaagtgcaag gacggcgtgc ggatcctcaa cgtcgcgcgc ggcccgctgc
    11461 tcgtcgactc cgacctccag gcggcgctgg actccggcaa ggtcggcggc gcggcgctcg
    11521 acgtcttcca gaccgagccg atcaccgagc acccgctctt cggccatccg agcgtcgtcg
    11581 tcacgccgca cctcggcgcc tcgaccgcag aggcgaccga tcgcgccggc taccaggccg
    11641 ccgagcaggt gatcgcggcg ttgaccggcg gcacggtgac gaacgccgtc aacgcgcccg
    11701 ccgtcgcggg cgaggacctc gaggcgctcg gaccgttcct accgctggcc gcccacctcg
    11761 gccggatcgc cgccacgctc accgagggga cgagcgtcga cggcatcgag gtcgaggtgc
    11821 tcggccggct cgccgagcgc gacacccgcc tcgtcaccgt ccaggtcatc aagggagtgc
    11881 tggccggcca cacggaggag gaggtcaacg acgtcaacgc tccgacgctc gccgaggagc
    11941 gcgggatcgc cgtcagctcg acgaccagcg agcgcgcgcg cgacttcacc gacctcgtcc
    12001 gggtcaccgt cgtcagcggg tccgaccgcg cacgcgtcgt cgggacggtg ctcggcaacc
    12061 gcgaccggcc gcacctgctc gaggcctggg gctcgcgctt caacgtccag ctcgagccgc
    12121 agctcgcgat cttccgctac cgcgaccagc ccggaatgat cggccgtgtc ggcacggtgc
    12181 tcggcgaggc cggcgtcaac atcgtctcgg cggccgtcgg ccgccagccc gacgacgccg
    12241 cgaccggcga ggccgtgatg gtcgtgaccg ccgatgccga agtgccgcag gacgtggtca
    12301 acgcgatcgt cgcctccgac ggcttcgtcg ccggccgcac cgtcagcctg gtctgagcct
    12361 tgacggctgc aggccgttcg ataccctggg acgagatgtc gttcgtgctc gacgcgcagc
    12421 ttctccttct cctcccccct cggggggact aatgcgtggc ggccgctaag ccgcaaagca
    12481 caggagaagc aggtcgagat ctagaaggag taggacgaca tggcgacatc agagacacgt
    12541 gacgaagcga gccgggtacg gatcttcgac acgaccctgc gcgacggcga gcagtcgccc
    12601 ggcatctcgc tgaacacgca ggagaagctc gagatcgccc accaactcgg acggctcggc
    12661 gtcgacatca tcgaggccgg cttcccgatc gcctcgcctg gcgacttcga ggcggtccag
    12721 gcaatcgcgc gcgaggtcca cggaccgatc atctgcgggc tcgcgcgcac cggcgtgcag
    12781 gacatcgacg ccgcttggaa cgcggtcaag gactccgagc ggccgcggat ccacaccttc
    12841 atctcgacca gcgacatcca catccagcac cagctgcaga ccacccgcga ggacgtcaag
    12901 gggcaggccc gcgcggccgt cgcgcacgcc aagggctact gcgacgacgt cgagttttcg
    12961 ccgatggacg ccacccgcgc cgacctcgag ttcaccgccg aggtgctgca gatcgcgctc
    13021 gacgagggcg cgacgacgat caacgtgccc gacaccgtcg gctactcgat gcccgaggag
    13081 tacgcgtcga tgtggcgcga gctctaccgg ctggtgcccg gcctcgccga cgtcgtgacc
    13141 tccgtccact gccacgacga cctcggcctg gcggtcgcca actcgttcgc cggcgtccag
    13201 gcgggctgcc ggcaggtcga gtgcgcgatc aacgggctcg gcgagcgggc cggcaacgcg
    13261 tcgctcgagg agatcgtgat gctgctcgcg gtccgcgagg ccgagcacgg gctctggacc
    13321 ggcgtcgaga cgaccgagat cgcgcgcacc agccgcctcg tctcgcggct gaccggctac
    13381 caggtgcagc cgaacaaggc gatcgtcggg cgcaacgcct tcgcgcacga gtccggcatc
    13441 caccaggacg gcgtgctcaa ggagcgctcg acctacgaga tcatggacgc gacgacgatc
    13501 gggctcgagt cgaactcgat cgtgctcggc aagcactccg gccgccacgc gctgcgcaag
    13561 gcgctcgagg agctggggtt cacggtcgac ggcgcggcgc tgaacaccgc cttcaagcgc
    13621 ttcaaggaga tcgccgaccg caagtcgaac gtgaccgcgc tcgacctcga ggcgatcgta
    13681 tccgacgaga tgcgccagtc cgaaagcgcg ttcgagctcg agtccttcga cgtcgaggcc
    13741 gggtccgacc ggtcgccgtt cgcgcgcgtg cgggtgcgga tgccgggggg cgcggtcgcc
    13801 gacggcgagt tcaccgggga cgggccggtc gacgcgttct tcagcgcgct gaacgtggcg
    13861 acggggcgcg aggcgcggtt gaaggagtac cacgtgtcgg cggtgacggc ggggcgcgat
    13921 gcgctggcgg aggtgacggt gctgctggag ctcgatgggg tgacggcgca ggggcagggg
    13981 gtgtcgccgg acacgatgga ggcttcgggg cgggcttatc tgcgggctat caccaatgcg
    14041 ttggcggatt cgccggtggc tgaggcggag catcacatcg atgcggcggc gaagagtgcg
    14101 acgccttagg gcgtagggcg cgggcgctgg tggccgcggg tcctgtctcc gattttggtt
    14161 tgggtgctcg caagctgcgc gcccaaacgc aaaatcgtcg ccacccgcgg gtggggcgtt
    14221 tgggtgcctg agggacaatg gttgggtgga cttcacgtgg gttgatgggg agcggctgat
    14281 tcggttcggg gccggggtgc ttggggaggc tccggggctc ttggctcagc gggggttcga
    14341 ggggtatgcg ctgttgacga cggagcgggc ggtggctggg gtgcctgtgt tggctgaggg
    14401 cgcgtcgacg gtgctgcacg tgcccgtggg atcggtgccg gacattgccg cggcggtgtt
    14461 gccggatgtg ggcgatcggc cggtggtggc gctcggcggg gggagggtgg tcgatgcggc
    14521 gaaggcggtc gcgggcgtgg agcggttgcc gtgcgcggcg gtgccgacga cgttggcggg
    14581 gtcgccgatg acgccgtttc atcggctgcc ggctgggacc aggggtgggc agctggtgcg
    14641 accgtcgttg gtggtgtggg atccggagct gatcgggacg ctgccacggg agcagctggt
    14701 ggcgacgggg atgaacgctt tggcgcatgc gttcgagtcg ctttacgtgc cgcacgccaa
    14761 tccggtcgcg gagatggcgg cgctgcgggc ggcgtcgctg ttcggagagc agctccagcg
    14821 cgagtcgccg aacgtggtgg atgtggcgct agcggctctg ctggctggct atgcggtcgg
    14881 gacgactggc tttgcgatcc accatgcgct ctgtcagacg acggtgcggg tcgctgggct
    14941 gccgcatgcg caggtcaatg cgctcgtgct gccacatacg gcgggactga tggctagccg
    15001 cgcccccgcc gcggtcgggc gctttgccac ggcgctcggt gacgttgccg gcgatccgct
    15061 ggccgcgccg aatcggatcg gcgagctggc cgcggggatc ggcgtgggag ggctggcgga
    15121 gctcggcctc gactcagatt cgatttcggc gatcgcccac gcggcgatcg ggcatccggc
    15181 gatcggcaac acgccgggcg gcacgccggc ggaggtcgat ctcgcgggca tcctcactgc
    15241 cgctctctga cggcgctgag atcgaccgag ggttgctagg cggcttggcg gacctggcgg
    15301 gctgggccct cagcgagtcg ttgctgctgg cgctcaggcg agatggtgat gacgttccag
    15361 gcgaggccga gcttctcgag cgcgcggatg gttaggccgg agatgtcgat ctcccaccag
    15421 cgcatgccct ggacggcggc gcgtgggaag gcgtggtggt tgtggtgcca ggcctcgccg
    15481 agcgagggga gggcgagcca ggcgacgttg gtcgagcggt cgtcggtctg gaagcggcgc
    15541 tcgccgaaga agtggcagat cgagttgatg ctccaggtga cgtggtggac gaagaagaca
    15601 cgcaccaggc cgccccagaa gagcgccagc gccgcggcct cgagcgttcc gccgatcgcg
    15661 aagccaagcc cgaacgggat cgcgaggccg aggatcaccc accagaggaa gtggcggctg
    15721 atgaagcgca tgccggggtc ctcgagcagg tccggcgcgt agcgcttggc gtcggcaacg
    15781 ccgttgtgct cgaacagcca cccgacgtgg gcgtgccaga ggccgcgcag gccgctgccc
    15841 tcgccgacgt gcgggctgtg cgggtcgccc tcctcgtcgg tgtgggcgtg atgcttgcgg
    15901 tggtcggcga cccagccgat caccgggccc tggacggcca ttgagccgag gatcgcgaag
    15961 aggtacttca gcggccggtg ggtctcgaag gcgcggtggg tgagcaggcg gtggaagccg
    16021 atcgtgatgc cgaacgcggt cgcgaggtag acgccgacca tgatcccgac gtcggtccag
    16081 ccgatcagcc ggttccagag cagcgggatc gcggctagga cggcgacgaa ggggacgatg
    16141 acggcggcga tgttcgcgtt tcgctggaga cgggtcatgg aactagaatc taggactaag
    16201 attgcttgtc tcctggccac tgagacaacg accgctccgc gagaacttgt agctttcaca
    16261 caatgaccgt gctcgaccag cggccctggg ttgcgttgcc gcccgcggcg gccgaggcgt
    16321 tgcggcccga gctgccggcg atcgcggagg agatcatcgg tgcgatcagc gacggcgtgc
    16381 cggagtacgc gctgccgctc gagggccggt ttggcgaggg gctgaggatg ggcgtcgagc
    16441 gggcgctgca gcgcttcctc gacctcagcg ggagcgacga cggcggtccc gaccccggcc
    16501 gccaggtcta cgtcgacctc ggacgcggcg agctgcgcgc gggccggccg ttggaggcgc
    16561 tgctcgcggc ctaccgcctc ggggcccgag tggcctggcg acggctggcg gcggcgggcg
    16621 agcgggcggg actcgagccg cgcacgctct atctgctcgc cgagtcgatc ttcgcctaca
    16681 tcgacgagct gtcgggcgag tcggtcgagg gctacgcgat ggagcaggct gcggtcgcgg
    16741 gcgcccagca gcgccgccgc cgcgagctcg ccgcgctgct cgtccaggac ccgccggcag
    16801 acgcgacggc gatcgaggcc gccgcgctcg ccgcgggatg gccacttccg cgcaccgtcg
    16861 ccgcggtcta cgtcgaggcc ggcgcgggcg aggcgccggc cgacccggac cggctctcca
    16921 accggctcgg ggcgggaacg atcgcggccg agctggctcc gggggcctgc gcgctcgttc
    16981 cggaccccga cgcgccgggc cgccgcgccc agctggccgc ggcgtttcgg gacggcgtcg
    17041 cggcgctcgg gccgtcggtg ccctggcgcg agggccacgt cagcgccggg cgcgctcgcg
    17101 cggcgctcgg c
//
LOCUS       DATABV010000212         6061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1275710, whole genome shotgun sequence.
ACCESSION   DATABV010000212 DATABV010000000
VERSION     DATABV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275710"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VHF88_09795"
     CDS             complement(<1..881)
                     /locus_tag="VHF88_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2162098.1"
                     /translation="MEASALHQSGLAASAGRSPLLRLRSDEQLIALTRRGNQSAFETL
                     VQRYQSRLLAFCRHMLSSKEDAEDVLQEVFAASYNALMADQRPINTRPWLYRIARNRC
                     LNHLRKPRPSGQDSMDIFERDNGTSTADTVHRREEFRQIVADVGLLPESQRSALLLRE
                     IDALSYEQIAEAMDTTVPSVKSLLVRARVSLAEAAEARLLTCAEVRLELGQVAEGLAK
                     TTAPVRRHLKGCRQCRVFRGELRHTTKALAAIAPVGPLLIILNNADPERTAKFFDDVT
                     AMGVGSIMVSPGYAYERA"
     gene            complement(953..1036)
                     /locus_tag="VHF88_09800"
     tRNA            complement(953..1036)
                     /locus_tag="VHF88_09800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1000..1002),aa:Leu,seq:taa)
     gene            complement(1066..2085)
                     /locus_tag="VHF88_09805"
     CDS             complement(1066..2085)
                     /locus_tag="VHF88_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX2162099.1"
                     /translation="MSATTREIRLASRPQGEPTDENFELAEVPLPEPGPGEVLVRNSY
                     LSVDPYMRGRMRDVKSYIPPFQVGEVLEGGAVGQVVASNDGRFGEGAWIQHMGGWREH
                     ALVGDGALAYPVDPAVAPIGTSLGVLGMPGLTAYAGVTEVAPVKEGETVYVSAAAGAV
                     GSVAGQIARLRGCRVVGSAGSAEKVDWLIGELGFDSAFDYHGVELPAALREHCPKGID
                     VYFDNVGGDHLSAALGRMRLHGRVALCGAVAEYNEETPRPGPDNFVGVIPRRLNLRGF
                     IVFDHGHLMKPFLSEVGAWVRSGELRYRETVVEGIENMPSAFNGLLAGDNIGKMLVKV
                     GPDPA"
     gene            2118..3767
                     /locus_tag="VHF88_09810"
     CDS             2118..3767
                     /locus_tag="VHF88_09810"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEX2162100.1"
                     /translation="MATTEQGSGTQLHGGRLVAKRLKAHGVSKLFTLSGGHIFSIYDG
                     CREEGIDIVDVRHEQSAAFAAEGWAKATREPGVCALTAGPGVTNGMSPLGSALMNRSP
                     MLMLGGRAPAMRWGMGSLQEIDHVPFVAPLTRLAKTAESTAEIPGLVDEALAAAAGPP
                     GGPAFLDFPLDYVFMEADVDADGPAAEVDPLLVPAAEGIEEAAALLRAAERPVIMAGS
                     GLYWAHGEHALLALAEELRIPVFVNGLARGCVPADHELAFSRARGQGLKGADVAVVIG
                     VPMDFRLGFGESFGEETKIVVVDSCAPERQHPRPVAGDLVGGVPATLDALRAAAAGGP
                     DRSGWIETLQASENEKRAAEQEELNDPRAPLHPLRLYNELAQVLDRSAVVIGDGGDFV
                     SYAGRVIETYEPGCWMDPGPFGCLGAGPGYALAAKLANPDRQVCLLMGDGAFGFSGME
                     FDTLVRHGVPVVAVMGNNGIWALEKHPMEFLYGYSVAAELQPECRYDQVVEALGGHGE
                     LVREPGELKPALERAFASGKPALVNVLTDPAVVYPRRSNLA"
     gene            3984..4451
                     /locus_tag="VHF88_09815"
     CDS             3984..4451
                     /locus_tag="VHF88_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2162101.1"
                     /translation="MHNVPTASETAGRGERERFDDAWEQFTAAVRRARSRVADRPDSK
                     LTPAQFRLLAALEGAPSLSVGELAQAAGVSSPTATRMLDGLEHDGIVKREAVPGDRRR
                     TAVRPTAKGRRALAETRKRVDAAREQIYAQLSPEERAQAERLLVRLAEAVEQI"
     gene            4448..5863
                     /locus_tag="VHF88_09820"
     CDS             4448..5863
                     /locus_tag="VHF88_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2162102.1"
                     /translation="MTTRDDDGRLHPNITLGVLATAAVAYAVSQTMVVPALPEIQREL
                     GVSQTQVTWVLTIYLLTASIATPIVGRLGDIYGKARMLSVTLVLFAVGLMVAALSHSL
                     ELLVAGRAIQGCGGAVFPLAFGIIRDEFPREKVGTAIGLISATFGIGGGAGLVLSGLI
                     VDHMAYEWIFWLAFAVVVGSLAATRIWVPESPVRAPSRIDWGGAALLSAGLVLVLLAL
                     SEGNAWGCGSARIVALLVAGTAVLAVWGWFETRVDDPLVDMRMMRRRPVWTTNLTGFL
                     VGFGMFGSFILTPQLVETPAIAGYGFGATVTEAGLFMLPSTAAMLVAGPLSGWLGTKV
                     GFRVPVLLGTAAAALSFLWLALLHDEPWQIVFGVALLGVGIGLSFAAMANLIVEAVEP
                     TETGIATGMNTIMRTLGGSLGGQIAASIVAAQVVAGTSFPAESGFTWAFAMSAVGLAL
                     AFGAALAIPRVRRRARVPVPA"
BASE COUNT          802 a         2005 c         2352 g          902 t
ORIGIN      
        1 gcccgttcat aggcatagcc gggcgagacc atgatggagc cgacccccat ggcggtgacg
       61 tcgtcgaaga atttcgccgt ccgctcgggg tccgcgttgt tgaggatgat cagcagcggt
      121 cccacgggcg cgatcgcggc gagcgccttc gtcgtatggc gcagctcgcc gcggaacacg
      181 cggcactggc ggcagccctt gaggtggcgg cggaccggcg cggtcgtctt ggcgaggccc
      241 tcggcgacct ggccgagctc gagccgcacc tcggcgcagg tcagcagccg cgcctcggcg
      301 gcctcggcga ggctgacgcg ggcgcgtacg agcagcgact tgacgctcgg cacggtggtg
      361 tccatcgcct cggcgatctg ctcgtaggag agcgcgtcga tctcgcgcaa cagcagcgct
      421 gagcgctggc tctcgggcag caggccgacg tcggcgacga tctggcggaa ctcctcgcga
      481 cggtggacgg tgtcggcggt cgacgtgccg ttgtcgcgct cgaagatgtc catcgagtcc
      541 tggcccgacg ggcgcggctt gcgcaggtgg ttgaggcagc ggttgcgcgc gatccggtag
      601 agccagggac gcgtgttgat cggccgctgg tcggccatca atgcgttgta ggaggcggcg
      661 aagacctcct ggaggacgtc ctcggcgtcc tccttggagc tcagcatgtg gcggcagaag
      721 gccagcaggc gcgactggta gcgctggacg agcgtctcga aggcggactg gttgccgcgc
      781 cgggtcagcg cgatcagctg ctcgtcgctg cgcaggcgta gcagcggcga ccgcccggcg
      841 gacgccgcaa ggcccgattg atgtagtgcg gaagcctcca tcaccccagg gcgtacccgt
      901 tccccaccct caagacacgg tagcgacggc aatgttgcgg tcaggttgag agtgcccggg
      961 acgggattcg aacccgcaca ccccgagggg tcacgcgttt taagcgcgct gcctggacca
     1021 attcggctac ccgggcaggt cgatggtaga cgggcgcggg gaccgtcagg cggggtcggg
     1081 gccgaccttg acgagcatct tgccgatgtt gtcgccggcc agcagcccgt tgaaggcgct
     1141 cggcatgttc tcgatcccct cgacgaccgt ctcgcggtag cgcagctcgc cggagcgcac
     1201 ccaggcgccg acctcgctca ggaacggctt catcaggtgg ccgtggtcga agacgatgaa
     1261 gccgcgcagg ttgaggcggc gcgggatcac tccgacgaag ttgtccggcc cgggccgcgg
     1321 cgtctcctcg ttgtactcgg cgaccgcgcc gcagagggcg acccggccgt gcaggcgcat
     1381 ccggccgagc gcggcggaga ggtggtcgcc gccgacgttg tcgaagtaga cgtcgatgcc
     1441 cttcgggcag tgctcgcgca gcgccgcggg cagctcgacg ccgtggtagt cgaaggcgga
     1501 gtcgaagccg agctcgccga tcagccagtc gaccttctcg gccgagccgg cgctgccgac
     1561 gacgcggcag ccgcgcaggc gggcgatctg gccggcgacg ctgccgaccg caccggcggc
     1621 ggccgacacg tagaccgtct ccccctcctt gaccggcgcg acctcggtga cgccggcgta
     1681 ggcggtcagg cccggcatgc cgagcacgcc gagcgacgtg ccgatcggcg cgaccgcggg
     1741 gtcgaccggg taggcgagcg cgccgtcgcc gaccagcgcg tgctcgcgcc agccgcccat
     1801 gtgctggatc caggcgccct ccccgaagcg gccgtcgttg gaggcgacga cctggccgac
     1861 cgcgccgccc tcgagcacct cgccgacttg gaacggcggg atgtaggact tgacgtcgcg
     1921 catccgcccg cgcatgtagg ggtcgaccga gaggtagctg ttgcggacga gcacctcacc
     1981 tggccccggc tcgggcagcg ggacctccgc gagctcgaag ttctcgtcgg tcggctcgcc
     2041 ctgcgggcgg gaggcgagac ggatctcgcg ggtggtggcg ctcataggga aacgacccta
     2101 gggcttagga tcgagcgatg gcgactacgg agcaggggtc aggcacgcag ctgcacggag
     2161 ggaggctcgt cgcgaagcgc ctcaaggcgc atggggtgag caagctgttc accctctcgg
     2221 gcggccacat cttctcgatc tacgacggct gccgcgagga ggggatcgac atcgtcgacg
     2281 tccgccacga gcagtcggcg gcgttcgcgg cggagggctg ggcgaaggcg acgcgcgagc
     2341 cgggcgtctg tgcgctgacc gccggcccgg gcgtgaccaa cggcatgagc ccgctcggct
     2401 cggcgctgat gaaccgctcg ccgatgctga tgctcggcgg ccgcgcgccg gcgatgcgct
     2461 gggggatggg ctcgctgcag gagatcgacc acgtgccgtt cgtcgcgccg ctgacccggc
     2521 tggcgaagac ggccgagtcg acggcggaga tcccgggact cgtcgacgag gcgctcgccg
     2581 ccgcggcagg gccgcccggc gggccggcgt tcctcgactt cccgctcgac tacgtcttca
     2641 tggaggccga cgtcgacgcc gacgggcccg ccgccgaggt cgacccgctg ctggtgccgg
     2701 cggccgaggg gatcgaggag gcagcggcgc tgctgcgcgc ggccgagcgg ccggtgatca
     2761 tggcgggcag cgggctctac tgggcgcacg gcgagcacgc gctgctcgcg ctcgccgagg
     2821 agctgcggat cccggtgttc gtcaacgggc tggcgcgcgg ctgcgtcccg gccgaccacg
     2881 agctcgcctt ctcgcgcgcc cgcgggcagg ggctgaaggg cgccgacgtc gcggtcgtga
     2941 tcggcgtgcc gatggacttc cggctcgggt tcggcgagtc gttcggcgag gagaccaaga
     3001 tcgtggtcgt cgactcctgc gctccggagc gccagcatcc ccggccggtc gcgggcgacc
     3061 tggtcggcgg cgtgccggcg accttggacg cgctgcgcgc ggcggcggcc ggcgggccgg
     3121 atcgctcggg ctggatcgag acgctgcagg cgagcgagaa cgagaagcgc gccgccgagc
     3181 aggaggagct caacgacccg cgcgcgccgc tgcatccgct gcggctctac aacgagctgg
     3241 cgcaggtgct ggatcgcagc gcggtcgtga tcggcgacgg cggcgacttc gtgtcgtacg
     3301 cgggccgggt gatcgagacc tacgagccgg gctgctggat ggaccccggg ccgttcggct
     3361 gcctcggcgc cgggccgggc tatgcgctgg cggcgaagct ggccaacccc gaccgccagg
     3421 tctgcctgct gatgggcgac ggcgcgttcg gcttcagcgg gatggagttc gacacgctcg
     3481 tgcgccatgg cgtcccggtc gtcgcggtga tgggcaacaa cggcatctgg gcgctcgaga
     3541 agcatccgat ggagttcctc tacgggtact cggtcgcggc ggagctgcag ccggagtgcc
     3601 gctacgacca ggtggtcgag gcgctcggcg ggcacgggga gctcgtgcgc gagccgggcg
     3661 agctgaagcc ggcgctggag cgcgcgttcg cgagcggcaa gccggcgctg gtcaacgtgc
     3721 tgaccgatcc ggcggtggtg tacccgcgga ggtcgaacct ggcgtagcgc gcgagccgcc
     3781 gcggcgcggc gagccgttcc gcggcgccga tgaagggttg tgccgcctgg gtgaggcgaa
     3841 tgcttcagcg gcggcgcgac ggcagtcgct ggcccgcccg acgaaggatt gtgccatctg
     3901 ggtgatgcag atgcttcggt gggcggccga ggcggtcggc cgtgataaat agcttcgcta
     3961 acgattagct agactaccga tcggtgcata acgtgccgac agcttccgag acggccggcc
     4021 gtggcgagcg ggagcgcttc gacgacgcgt gggagcagtt cacggcggcg gtgcgccggg
     4081 cgcgcagccg cgtagccgat cgtcccgatt cgaagctgac gccggcccag ttccggctgc
     4141 tggcggcgct cgagggtgcg ccgtcgctga gcgtggggga actggctcag gcggccggcg
     4201 tctcttcgcc gaccgccacg cggatgctcg acggccttga gcacgacgga atcgtcaagc
     4261 gcgaggcggt gcccggcgac cgccgccgga cggcggttcg gccgacggcg aagggccggc
     4321 gcgcgctggc agagacgcgc aagcgcgtcg acgccgcgcg cgagcagatc tacgcgcagc
     4381 tctcgcccga ggagcgcgcg caggccgagc ggctgctggt ccggctcgcg gaggcggtcg
     4441 agcagatatg accacgcgcg acgacgacgg ccgcctgcac ccgaacatca cgctcggcgt
     4501 actcgctacc gcggcggtcg cctacgcggt ctcgcagacg atggtcgtgc cggcgctgcc
     4561 ggagatccag cgtgagctgg gcgtttcgca gactcaggtc acgtgggtgc tcaccatcta
     4621 cctgctgacc gcctcgatcg cgacgccgat cgtcggtcgg ctcggcgaca tctacggcaa
     4681 ggcgcgcatg ctgagcgtga cgctggtgct gttcgcagtc ggcctgatgg tcgccgccct
     4741 gtcgcactcg ctcgagctgc tggtggccgg gcgggcgatc cagggatgcg gcggcgcggt
     4801 cttcccgctc gccttcggga tcatccgcga cgagttcccg cgcgagaagg tcggcacggc
     4861 gatcggactg atcagcgcga cgttcgggat cggcggcggg gccggcctgg tcctcagcgg
     4921 cctgatcgtc gaccacatgg cctacgagtg gatcttctgg ctggcgttcg cggtcgtcgt
     4981 cggctccttg gccgcgacca ggatctgggt gccggagtcg ccggtgcgcg cgccgtcgcg
     5041 gatcgactgg ggcggggcgg cgctgctgtc ggccggcctg gtgctggtgc tgctcgcgct
     5101 cagcgagggg aacgcgtggg gctgcggctc tgcgcggatc gtcgcgctgc tggtcgcggg
     5161 gacggcggtg ctggccgtct ggggctggtt cgagacgcgc gtcgacgacc cgctggtcga
     5221 catgcggatg atgcgtcggc gcccggtctg gacgacgaac ctgacgggct tcctggtcgg
     5281 cttcggcatg ttcggctcgt tcatcctcac gccgcagctc gtcgagacgc cggcgatcgc
     5341 cggctacggc ttcggcgcga ccgtgaccga ggcggggctg ttcatgctgc cgtcgaccgc
     5401 cgcgatgctg gtcgccggcc cgctgtcggg ctggctcggc acgaaggtcg gcttccgcgt
     5461 accggtgctg ctcgggaccg ccgcggcggc gctgtcgttc ctgtggctgg cgctgctcca
     5521 cgacgagccc tggcagatcg tgttcggcgt cgcgctgctc ggggtcggga tcgggctgtc
     5581 gttcgcggcg atggcgaacc tgatcgtcga ggcggtcgag ccgaccgaga cggggatcgc
     5641 caccggcatg aacacgatca tgcggaccct cggcgggtcg cttggcgggc agatcgcggc
     5701 gagcatcgtg gcggcgcagg tcgtggccgg gacgtcgttc cccgccgagt cgggcttcac
     5761 gtgggcgttc gcgatgtccg ccgtcgggct ggcgctggcc ttcggcgcgg cgctggcgat
     5821 cccgcgggtt cggcggcggg cgcgcgtgcc ggtcccggcc tagagatcct gcctagaaat
     5881 agagccgccc ggacgagtcc gggcggctcg acgacccagt caggacgggc aggggtacat
     5941 gtcttgaatg ggtctgacga ctcggacggc gggaggcgcc gatacatgcg gctaacgtcc
     6001 gagcctatgt accgggttag gagccgccgt gcgtttccgc atggtcttcc gtcccggcag
     6061 g
//
LOCUS       DATABV010000213         6645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2800918, whole genome shotgun sequence.
ACCESSION   DATABV010000213 DATABV010000000
VERSION     DATABV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800918"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VHF88_09825"
     CDS             <1..620
                     /locus_tag="VHF88_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEX2162103.1"
                     /translation="PETHIATLEKKTKLEVYLTIGHGSGYSPAEDNKSPEQPIGVIPI
                     DSIFSPVKRVAYSVESARVGQRTDYDKLTLDIETDGSLDPHAALREAAETLIKYLAIF
                     SEPERVESLREAGAALVGAAAVNGGDLGGGAVSGGGDGMDDILIEELELGVRSYNCLK
                     RAGVQTVGDLIQKSESELNAIPNFGRKSIEEVMETLEARGLSLRS"
     gene            651..1004
                     /gene="rplQ"
                     /locus_tag="VHF88_09830"
     CDS             651..1004
                     /gene="rplQ"
                     /locus_tag="VHF88_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010285110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX2162104.1"
                     /translation="MRHQKQKGKLSRDAAHRKALLMNLSREIIDHERIKTTQAKAKAV
                     KPELERLITLAKKGDAHSRRLAMARLGQDKGVVYKLFEEIAPRYAERPGGYLRISKLG
                     PRRSDATEMVYLELV"
     gene            complement(1133..1714)
                     /locus_tag="VHF88_09835"
     CDS             complement(1133..1714)
                     /locus_tag="VHF88_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2162105.1"
                     /translation="MKLLAFSDVHCDLGAIDSLVDQARSADIVAGVGDFASVHEGLEQ
                     TIAPLRTIDAPLLLVPGNNETADALRAATAGWDATTVLHGQGTQIDGIDFYGIGAGIP
                     TTPWDWSFDLTDDEAASMLTALPERAILLSHSPPKGHVDKNLGSTAVLGAAQAKNARA
                     VLCGHIHEEWTNESHIGPTLVRNLGPDGYTLDL"
     gene            1749..2546
                     /locus_tag="VHF88_09840"
     CDS             1749..2546
                     /locus_tag="VHF88_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2162106.1"
                     /translation="MDLERIKQGAREGWGKGDYSGLSEVLRPAAQALCDACAVGAGQE
                     VLDVAAGDGNFALACAFEGASVVASDISPGMVERGRARSEAEGYDVEWVEADAEDLPF
                     EDGRFECVGSVFGAVMAPRPDVVARELFRVVRPGNTVGLVAWVPDGISQEMFAIGRKY
                     APPSDAPTSELWGDEDVVRERFGELANSIEMERRSLPWSGESPEAFVDHMERHAPMQA
                     AAKERMPAERYAEMRAEFLEMVRGWAGGDGPFSVDAEYLLVVARKRG"
     gene            2546..3313
                     /locus_tag="VHF88_09845"
     CDS             2546..3313
                     /locus_tag="VHF88_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEX2162107.1"
                     /translation="MAARLLIEYDGGKFAGWARQPGQRTVQEVLERALAQQASRPVAL
                     TVAGRTDAGVHARGQVASHDGESQPRERVNALLPPDVRLLESEAAPDGFDARRDATGR
                     VYRYRVLPGGVERPLERGRALHWRYPIERGVLDECAVLLPGRHDFTAFTPTDGYHRRF
                     ERDVVRAEWVEEPGGVLAFWIEADSFMRHMVRTLVGTMLGLAAGRTATIAPFASLLEG
                     RHRREAGDTVQAHGLYLEAIRYGDSRLRREGYPRSSR"
     gene            3310..4128
                     /gene="surE"
                     /locus_tag="VHF88_09850"
     CDS             3310..4128
                     /gene="surE"
                     /locus_tag="VHF88_09850"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX2162108.1"
                     /translation="MKVLLTNDDGIQATGLHAMRRALLEVPGVELAVIAPDSNRSATA
                     RSITTRVPLWVEEVELGDGTIGHATDGTPVDCVRFAALGLIEFKPELIVSGINHGSNL
                     GDDITYSGTVAAALEGIVLGVPAIAVSQQADHGAMDFRIGRAWEPENFEQAAAFVARI
                     VEELDHVPMPERTLLNVNVPAGEIKGARACRLGKRIYRDSLELAEEQDGRRRYRIYGE
                     SPGYHPEEGTDFAAIADGRIAVTPLHFDLTDRAAMETLGSFDFDRLLAPAAREV"
     gene            4125..6152
                     /gene="ligA"
                     /locus_tag="VHF88_09855"
     CDS             4125..6152
                     /gene="ligA"
                     /locus_tag="VHF88_09855"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX2162109.1"
                     /translation="MSASLEERAAELRRQIEHHNRLYYQLDEPEIGDDEYDALIDELR
                     RIEAEHPELKSVESPTQRVGGSPLQRFEPVRHEQPMLSLANARSEEELRAWLERTERY
                     IERQEIEHEGDICFVTEPKIDGLAISLLYRDGELMRGATRGDGEVGEDVTQNLRTVET
                     VPQRVEGAPPLLEVRGEIYLPRGAFAKLNEERAAAGEPTYANPRNTAAGSIRQLDPAL
                     AAARPLALWSYSIGALEGLRFASHYESLEWLREHGFPVSPDVERHADLDAVVATCRAW
                     EERREGLDFEIDGVVVKVDDLALQRRLGVVGREPRGAIAWKFPPMTANTVLRRIQWNV
                     GRTGHMVPFAELEPVQVSGVTVKLATLHNEEDIVRKDVREGDEVIVTRAGDVIPRVVS
                     PTPQAQRNPDRGPVPAPPAECPACGTPTVKPEGGVWTICPNRAGCPGQLFQTVKQFVS
                     RGAMDIEGLGEERVLQFLREGVIDGIAGIYEITEERLVELERFGPGSARNLVEAIERS
                     KRQPFHRVLFGLGIHGIGGVNARALAAHFRTIDALLAATPEQIEEVPGIGPILAEQIA
                     ETLSEDATRELLERLRAHGLQLEEEGPPPGAVDGPLNGMTLVLTGTLPDLTREDATER
                     IEAAGGKVTGSVSKKTDYVVAGADPGSKLAKAEKLGVEVIDEDGLLKLLAG"
     gene            complement(6158..>6645)
                     /locus_tag="VHF88_09860"
     CDS             complement(6158..>6645)
                     /locus_tag="VHF88_09860"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX2162110.1"
                     /translation="PPPPPPPTTAVKTLTVPAPRVVTRGTTAGGAKIAARFAKRLGGG
                     PARLADGSGLSRLNRASPYRVVRLLLALRERDEFRAFSRSLSIAGRDGTLGSRMRSGP
                     ARGRCRGKTGTLTGVSAVSGYCRARSGDTYVFSILMNGVDPYGARSIQDRMLHALAGV
                     R"
BASE COUNT          989 a         2098 c         2611 g          947 t
ORIGIN      
        1 atcccgagac gcacatcgcg acgctcgaga agaagaccaa gctcgaggtc tacctgacga
       61 tcggccacgg cagcggctac tcgcccgccg aggacaacaa gtcgcccgag cagccgatcg
      121 gcgtcatccc gatcgactcg atcttctcgc ccgtcaagcg cgtcgcctac tccgtcgagt
      181 cggcccgcgt cggacagcgc accgactacg acaagctgac gctcgacatc gagaccgacg
      241 gctcgctcga cccgcacgca gcactgcgcg aggccgctga gacgctgatc aagtacctgg
      301 cgatcttcag cgagcccgag cgcgtcgagt cgctgcgcga ggccggcgcg gcgctcgtcg
      361 gggcggccgc ggtcaacggc ggcgacctgg gcggcggagc cgtctccggc ggtggcgacg
      421 ggatggacga catcctgatc gaggagctcg agctcggcgt ccgctcgtac aactgcctca
      481 agcgcgcggg cgtccagacg gtcggcgacc tgatccagaa gtccgagtcc gagctcaacg
      541 cgatcccgaa cttcggtcgc aagtcgatcg aggaggtcat ggagaccctc gaggcgcgcg
      601 gcctgtcgct gcgctcgtag gctcctgccg ctacgctgcc tgggttcgtc atgcgccatc
      661 agaagcaaaa gggaaagctc agccgtgatg cggcgcaccg caaggcgctg ctgatgaacc
      721 tctcgcgcga gatcatcgac cacgagcgga tcaagacgac ccaggcgaag gcgaaggcgg
      781 tcaagccgga attggagcgg ttgatcacgc tggcgaagaa gggggacgcg cactcgcgga
      841 ggctggcgat ggcgcggttg gggcaggaca agggtgtggt gtacaagctg ttcgaggaga
      901 tcgcgccgcg gtatgcggag cggccgggcg gctacctccg gatctcgaag ttggggccgc
      961 ggcggtcgga cgcgaccgag atggtttatc tcgagctggt ttagggctct gggctagggc
     1021 tcgggcgtgg ctgcccgcgg gtcctgtctc cgattttggt tcacgtgctc gcaagctgcg
     1081 cgcgcgaacg caaaatcgtc gccacccgcg gatgggatgc cggggttagg gcttagaggt
     1141 cgagggtgta gccgtctggg ccgaggttgc ggaccagggt ggggccgatg tgggactcgt
     1201 tggtccactc ctcgtggatg tggccgcaga ggacggcgcg ggcgttcttt gcctgagcgg
     1261 cgccgaggac ggcggtgctg ccgaggttct tgtcgacgtg gcccttggga ggggagtggc
     1321 tgagcaggat cgcccgctct gggagggcgg tgagcatgct ggcggcctcg tcgtcggtta
     1381 ggtcgaagct ccagtcccag ggggtggtgg ggatgccggc gccgatgccg tagaagtcga
     1441 tgccgtcgat ctgggtgccc tggccgtgga ggacggtggt ggcgtcccag ccggctgtgg
     1501 ctgcgcggag ggcgtctgcg gtctcgttgt tgccgggcac cagtaggagc ggcgcatcga
     1561 tcgttcgcag cggggcgatc gtctgctcga ggccctcgtg gacgctggcg aagtcgccga
     1621 cgccggcgac tatgtcggcg gagcgggctt ggtcgacgag ggagtcgatg gcgccgaggt
     1681 cgcagtggac gtctgagaag gcgaggagct tcatgtggga aagcctatcc cggtgatagc
     1741 ttcgcgggat ggacttggag cggatcaagc agggggctcg ggagggctgg gggaagggcg
     1801 actactcggg actgtcggag gtgctgcggc cggcggcgca ggcgctgtgc gatgcgtgtg
     1861 cggtgggcgc cgggcaggag gtgctggacg tggcggcggg ggatgggaac ttcgcgctgg
     1921 cgtgtgcgtt cgagggggcg agcgtggtgg cgtcggacat ctcgccgggg atggtcgagc
     1981 gcggtagggc gcggtcggag gcggaggggt acgacgtcga gtgggtggag gccgacgcgg
     2041 aggacctgcc gttcgaggac gggcggttcg agtgcgtcgg ctcggtgttc ggggcggtga
     2101 tggcgccgcg gccggacgtc gtggcgcggg agctgttcag ggtcgtgcgg ccggggaaca
     2161 ccgtcggctt ggtggcctgg gtgcctgatg ggatctcgca ggagatgttc gcgatcggtc
     2221 gcaagtatgc gccgccgtcg gacgcgccga cgagtgagct gtggggcgac gaggacgtcg
     2281 tcagggagcg gttcggcgag ctggcgaact cgatcgagat ggagcgacgg tcgttgccgt
     2341 ggagtggtga gtcgccggag gccttcgtcg atcacatgga gcgccacgcg ccgatgcaag
     2401 cggcggcgaa ggagcggatg ccggcggagc gctacgccga gatgcgcgcc gagttcctcg
     2461 agatggtccg tggctgggcc ggtggcgacg gaccgttctc ggtcgatgcc gagtacctgc
     2521 tcgtggtcgc gcgcaagcgc gggtagtggc cgcgcggctg ctgatcgagt acgacggcgg
     2581 gaagttcgcc ggctgggcga ggcagccggg gcagcggacg gttcaggagg tgctggagcg
     2641 ggcgttggcg cagcaggcga gcaggccggt ggcgctgacg gtggcgggac ggaccgacgc
     2701 gggcgttcat gcgcgtgggc aggtggcatc gcacgacggc gagtcgcagc cgcgcgagcg
     2761 ggtcaacgcg ctgctgccgc cggacgtgcg actgctggag agcgaggctg cgcccgacgg
     2821 gttcgacgca cgccgcgacg cgaccgggcg cgtgtaccgc taccgcgtgc tgccgggagg
     2881 ggtcgagcgc ccgttggagc gggggcgggc gctgcactgg cgctatccga tcgagcgcgg
     2941 cgtgctcgac gagtgcgcgg tgctgctgcc gggtcgtcac gacttcacgg cgttcacgcc
     3001 gaccgacggc taccaccgcc gcttcgagcg cgacgtggtt cgcgccgagt gggtcgagga
     3061 gccaggcggg gtgctggcgt tctggatcga ggccgactcc ttcatgcgcc acatggtgcg
     3121 gacgctggtc ggaacgatgc tggggctggc cgccgggcgg accgcgacga tcgcgccgtt
     3181 cgcgagcctg ttggagggcc gccaccggcg cgaggccggc gacacggtgc aggcccacgg
     3241 cctctacctg gaggcgatcc gctatggcga cagccgcctc cggcgcgagg gctatcctcg
     3301 gtcgtcgcga tgaaggtgct gctgaccaac gacgacggga tccaggcgac cgggctgcac
     3361 gccatgcggc gtgcgctgct cgaggtcccc ggcgtcgagc tggcggtgat cgcgcccgac
     3421 tcgaaccgct ccgcgacggc gcgcagcatc acgacgcgcg tgccgctgtg ggtcgaggag
     3481 gtcgagctcg gggacgggac gatcggccat gcgaccgacg gcacgccggt cgactgcgtc
     3541 cgcttcgcgg cgctcgggct gatcgagttc aaacccgagc tgatcgtctc cgggatcaac
     3601 cacgggtcca acctcggcga cgacatcacc tactcgggga ccgtcgcagc ggcgctcgag
     3661 gggatcgtgc tcggggtgcc ggcgatcgcc gtctcgcagc aggccgacca cggcgcgatg
     3721 gacttccgca tcggccgtgc ttgggagccc gagaacttcg agcaggcggc cgcattcgtc
     3781 gcgcggatcg tcgaggagct cgaccacgtg ccgatgcccg agcggacgct gctcaacgtc
     3841 aacgttccgg ccggggagat caagggggcc cgggcgtgcc ggctcggcaa gcggatctac
     3901 cgcgactcgc tcgagctggc ggaggagcag gacggccgcc gccgctaccg catctacggc
     3961 gagagccccg gctaccaccc cgaggaggga accgacttcg cggcgatcgc cgacggcagg
     4021 atcgcggtga cgccgctgca cttcgacctc accgatcgcg cggcgatgga gacgctcggc
     4081 agcttcgact tcgaccgcct gctcgcgccc gcggcacgcg aggtgtgagc gcctccctcg
     4141 aagagcgtgc ggccgagctg cgcaggcaga tcgagcacca caaccggctc tactaccagc
     4201 tcgacgagcc agagatcggc gacgacgagt acgacgcgct gatcgacgag ctgcgccgga
     4261 tcgaggccga gcatccggag ctgaagagcg tcgagtcgcc gacccagcgc gttggcggga
     4321 gcccgctcca gcgcttcgag cccgtccgcc acgagcagcc gatgctctcg ctcgccaacg
     4381 cgcgctcgga ggaggagctg cgcgcctggc tcgagcgcac cgagcgctac atcgagcgcc
     4441 aggagatcga gcacgagggt gacatctgct tcgtcaccga gccgaagatc gacggcctgg
     4501 cgatctcgct gctctatcgc gacggcgagc tcatgcgcgg ggcgacgcgc ggggacggcg
     4561 aggtcggcga ggacgtcacg cagaacctgc gcaccgttga gacggtccct cagcgcgtcg
     4621 agggagcccc tccgctgctc gaggtgcgtg gcgagatcta cctgccgcgt ggcgcgtttg
     4681 cgaagctcaa cgaggagcgc gcggcggcgg gcgagccgac ctacgcgaac ccgcgcaaca
     4741 ccgccgccgg ctcgatccgc cagctcgacc cggcgctcgc ggccgcgcgt ccgctcgccc
     4801 tctggagcta ctcgatcggc gcgctggagg gactgcggtt cgcgtcgcac tacgagtcgc
     4861 tggagtggct gcgcgagcac ggcttcccgg tcagccccga cgtcgagcgc cacgccgatc
     4921 tcgacgctgt cgtcgcgacc tgccgcgcct gggaggagcg gcgcgagggg ctcgacttcg
     4981 agatcgacgg ggtcgtcgtg aaggtcgacg acctcgcgct gcagcgccgg ctgggcgtcg
     5041 tcgggcgcga gccgcgcggg gcgatcgcct ggaagttccc gccgatgact gcgaacacgg
     5101 tcctgcggcg gatccagtgg aacgtcggcc gtacgggcca catggtcccg ttcgccgagc
     5161 tcgagccggt ccaggtctcg ggcgtcaccg taaagctggc gaccctccac aacgaggagg
     5221 acatcgttcg caaggacgtg cgcgagggcg acgaggtgat cgtcacgcgc gcgggcgacg
     5281 tgattccccg cgtcgtgtcg ccgaccccgc aggcgcagcg caacccggac cgcggccccg
     5341 tccccgctcc gccggccgag tgcccggcct gcggcacgcc gacggtcaag ccagaggggg
     5401 gtgtctggac gatctgcccg aaccgcgccg gctgtccggg tcagcttttc cagacggtca
     5461 agcagttcgt cagccgcggc gcgatggaca tagagggact cggcgaggag cgcgtcctgc
     5521 agttcctgcg cgagggcgtg atcgacggta tcgcgggcat ctacgagatc accgaggagc
     5581 ggctggtcga gctcgagcgg ttcgggcctg gctcggcccg caatctcgtc gaggcgatcg
     5641 agcgctccaa gcgccagccg ttccaccggg tgctgttcgg cctcggcatc cacgggatcg
     5701 gcggcgtcaa cgcacgcgcg ctggccgcgc acttccggac gatcgacgca ctgctcgcgg
     5761 cgacgcccga gcagatcgag gaggtgccgg ggatcgggcc gatcctcgcc gagcagatcg
     5821 ccgagacgct cagcgaggac gccacgcgcg agctgctcga gcgattgcgc gcccacggtc
     5881 ttcagctcga ggaggagggg ccgccgcctg gcgcggtcga tggcccgctg aacggcatga
     5941 ccctcgtcct aaccgggacg ctgcccgatc tgacgcgcga ggacgcgacc gagcggatcg
     6001 aggcggccgg cggcaaggtg accggctccg tgtcgaagaa gaccgactat gtcgtcgcgg
     6061 gggccgatcc cggctcgaag ctcgccaagg ccgagaagct cggcgtcgag gtgatcgacg
     6121 aggacgggct gttgaagctg ctggccggct gacttactca gcgcacgccg gcgagcgcgt
     6181 gcagcatccg gtcctggatg ctgcgcgcgc cgtaggggtc gacgccgttc atcaggatcg
     6241 agaagacgta ggtgtcgccc gagcgtgcgc ggcagtagcc ggagaccgcg gagacgccgg
     6301 tcagcgtgcc ggtcttgccg cggcagcgac cgcgggccgg tccgctgcgc atccggctgc
     6361 cgagagtgcc gtcacggccg gcgatcgaga gcgagcgact gaaggcgcgg aactcgtcgc
     6421 gctcgcgcag cgccagcagc agcctgacga cgcggtacgg cgaggcgcga ttgagccgcg
     6481 agaggcccga gccgtcggcg agccgtgcgg gaccgccgcc gaggcgcttg gcgaagcggg
     6541 cagcgatctt ggcgccgcct gcggtggtgc cacgggtgac gacgcgtggc gcggggacgg
     6601 tgagggtctt gaccgcagtg gtgggggggg gggggggggg ggggg
//
LOCUS       DATABV010000214         4041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1676549, whole genome shotgun sequence.
ACCESSION   DATABV010000214 DATABV010000000
VERSION     DATABV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676549"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /gene="eno"
                     /locus_tag="VHF88_09865"
     CDS             complement(<1..390)
                     /gene="eno"
                     /locus_tag="VHF88_09865"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX2162111.1"
                     /translation="MSAIEQVHARQILDSRGNPTVEVEVTLASGAFGRAAVPSGASTG
                     EFEAVELRDGGDAYLGKAVTTAVANANGELAQAVLGLDAGDQEAVDRAMIAADGTPNK
                     ARLGANAILGISLAVAKAAAAEAGQPLW"
     gene            complement(435..1109)
                     /locus_tag="VHF88_09870"
     CDS             complement(435..1109)
                     /locus_tag="VHF88_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX2162112.1"
                     /translation="MTERRAADLVEALQRLDEITWRLRRDCPWDREQDERSIVPHTIE
                     EAYELADAAHSGDDAKLLDELGDVLFQVMFLSMLLEERDAGSLAAVADHCREKLIRRH
                     PHVFGEAAAETAGEVLESWAQIKREQERAGEVFGDLPENLPATLYAKKVLRRAQSAGL
                     TPPVGPGEGGGPAPGDEAFEAIGRQLFEAVEAAGAAGVDPESALREHARRFRAEIERR
                     ADQAAQ"
     gene            complement(1114..2232)
                     /locus_tag="VHF88_09875"
     CDS             complement(1114..2232)
                     /locus_tag="VHF88_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX2162113.1"
                     /translation="MPSDAVARVGDTVITKQQFDHWYESAAKSQAQQGGPSVPPDPPN
                     FTKCIAALKEAQPKGSKPKDADLKKQCKQSYDQLKQQVMQFLIQSQWVQQEAEEQGVK
                     VSGKEVRTLFEDQKKQAFPKERDYQKFLRTSGATEQDILFRIKLDALQNKLTAKIQKD
                     QEKITDEDVEEYYEKNKKRFGQPERRDLNIVLTKTKAKAEQAKRELDGGAKFSAVAKK
                     YSIDQASKANGGKLPDVPQGQQEKAFDEAVFDAEQGEIEGPVKTQFGWYVFEVTDVTE
                     ASQQSLDDAKETIRGLLKSEREQKSLQDFIEDFREDFKDQTKCREGFVVAECSNAPEE
                     TTDTNAAPGAPQGAPQGAPPPPPQGAPQQAPPPQQPAP"
     gene            complement(2416..>4041)
                     /locus_tag="VHF88_09880"
     CDS             complement(2416..>4041)
                     /locus_tag="VHF88_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2162114.1"
                     /translation="DQLDAIEAVRADMEEARPMDRLICGDVGYGKTEVALRAAFKAAA
                     DSKQVMFLVPTTVLAQQHHGTFSERLRDYPFRIEQVSRFRSDKEVRAALKDFEDGKVD
                     ILIGTHRLLSRDVRPKDLGLLIVDEEQRFGVKQKELLRQLRLRVDVLSLSATPIPRTL
                     QMSLAGLRDISVIETPPEGRRPVKTYVGEYDEDLVQKAIERELERRGQAFFLHNRVDS
                     IDETAERVRAMCPKARVLVAHGQMEEGQLEGIMLAFLRGEADVLVCTTIVESGLDISN
                     ANTLIVERADELGLAQLYQIRGRVGRSKERAYAYLLYPSAAALTSEAGDRLATLSDYT
                     ELGSGFKIAMRDLEIRGAGNLLGDDQSGHVAAIGFELYVGMLDEAVAALAGDSAEEAP
                     EPVRVDLPVDAFVPGDYVPYEAAKIEIHRRVAGAKEIADLIVLREELEDRFGPVPEPL
                     DNLIKLQDARIKLGRAGVRTVDFAGGKLAVSPVELDSTQVSVLRERVPEAIYESGRST
                     VRVRVPDEPAERFAAVVRAAEAVLEAASEPARA"
BASE COUNT          509 a         1410 c         1441 g          681 t
ORIGIN      
        1 ccagagcggc tggccggcct ccgccgcggc ggccttcgcg acggcgagcg agatgccgag
       61 gatcgcgttg gcgccgagcc gcgccttgtt cggcgtgccg tcggcggcga tcatcgcgcg
      121 gtcgacggcc tcctggtcac cggcgtcgag ccccagcacc gcctgcgcca gctcgccgtt
      181 cgcattcgcg accgccgtcg tgaccgcctt gccgaggtag gcgtcgccgc cgtcgcgcag
      241 ctcgaccgcc tcgaactcgc ccgtcgaggc gcctgacggc accgcggcgc ggccgaacgc
      301 ccccgacgcc agcgtgacct cgacctcgac ggtcgggttg ccgcggctgt ccaggatctg
      361 acgggcgtgg acctgctcga ttgcgctcac gggacgggat ctacctcctg ggggtcggcg
      421 ggcggcggct gatcctattg cgcggcttgg tctgcgcggc gctcgatctc ggctcgaaag
      481 cggcgtgcgt gctcgcgcaa cgcggactcg gggtcgacgc ccgctgcgcc ggcggcctcg
      541 acggcctcga agagctggcg gccgatcgcc tcgaacgcct cgtcgcccgg agcgggaccg
      601 ccgccctcgc ccggtccgac cggcggcgtc aggccggccg actgggcgcg gcgcaggacc
      661 ttcttggcgt agagggtcgc cggcaggttc tccggcaggt cgccgaagac ctcgccggcg
      721 cgctcctgct cgcgcttgat ctgcgcccag ctctcgagca cctcgccggc ggtctcggcg
      781 gccgcctcgc cgaagacgtg cgggtggcgg cggatcagct tctcgcgaca gtggtcggct
      841 acggcggcga gcgagccggc gtcgcgctcc tcgagcagca tcgagaggaa catcacctgg
      901 aagaggacgt cgccgagctc gtcgagcagc ttggcgtcgt cgccggaatg ggcggcgtcg
      961 gcgagctcgt aggcctcctc gatcgtgtgc gggacgatcg agcgctcgtc ctgctcgcgg
     1021 tcccacgggc agtcgcggcg caggcgccag gtgatctcgt cgagacgctg gagcgcctcg
     1081 acgagatcgg cggcccgccg ctccgtcacg cgactacgga gcgggctgct gcggcggcgg
     1141 cgcctgctgc ggcgcgccct gtggtggcgg cgggggagcg ccctggggcg cgccctgcgg
     1201 cgcaccgggc gccgcgttgg tgtcggtcgt ctcctcggga gcgttgctgc actccgcgac
     1261 gacgaagccc tcgcggcact tggtctggtc cttgaagtcc tcgcggaagt cctcgatgaa
     1321 gtcctgcagc gacttctgct cgcgctcgga cttcagcagg ccgcggatgg tctccttggc
     1381 atcgtcgagc gactgctggc tcgcctcggt gacgtcggtg acctcgaaga cgtaccagcc
     1441 gaactgggtc ttgaccgggc cctcgatctc gccctgctcg gcgtcgaaga ccgcctcgtc
     1501 gaacgccttc tcctgctggc cctgcgggac gtcgggcagc ttgccgccgt tcgccttcga
     1561 ggcctggtcg atcgagtact tcttcgcgac cgcggagaac ttggcgccgc cgtcgagctc
     1621 gcgcttggcc tgctcggcct tcgccttggt cttggtcagg acgatgttca ggtcacggcg
     1681 ctccggctgg ccgaagcgct tcttgttctt ctcgtagtac tcctcgacgt cctcgtcggt
     1741 gatcttctcc tggtccttct ggatcttcgc ggtcagcttg ttctgaagcg cgtcgagctt
     1801 gatccggaag aggatgtcct gctcggtggc gccggaggtg cggaggaact tctggtagtc
     1861 gcgctccttc gggaaggcct gcttcttctg gtcctcgaag agcgtccgga cctccttgcc
     1921 gctgaccttg acgccctgct cctcggcctc ctgctggacc cactgcgact ggatcaggaa
     1981 ctgcatcacc tgctgcttga gctggtcgta gctctgcttg cactgcttct tgaggtcagc
     2041 gtccttgggc ttcgagccct tgggctgcgc ctccttgagc gcggcgatgc acttggtgaa
     2101 gttcggcggg tcgggcggga ccgatggacc gccctgctgc gcctggctct tggccgcgct
     2161 ctcataccag tgatcgaact gctgcttggt gatgacggtg tcgccgacgc gcgctacggc
     2221 gtcggaaggc acgtcggacc caccgcagcc ggaagcggcg acggcgcaga tgccagcgag
     2281 cgatacgccc gcgataagac gaagaaagtt catggaggct gagttcatcc cgaggtagct
     2341 gatgcgcccc tcctcgaggg cgcctgaaag gcagcggttg actgtagcgg agcgctgcgg
     2401 gctctgtcgc gggtcttagg cgcgcgccgg ctcgctggcc gcctcgagca ccgcctcggc
     2461 cgcgcggacg acggcggcga agcgctctgc cggctcgtcg gggacgcgga cgcggacggt
     2521 gctgcgcccc gactcgtaga tcgcctcggg cacgcgctcg cgcagcaccg acacctgcgt
     2581 ggagtcgagc tcgaccggcg agaccgcgag cttgccgccc gcgaagtcga cggtccggac
     2641 gcccgcgcgg ccgagcttga tgcgggcgtc ctggagcttg atcaggttgt cgagcggctc
     2701 cgggacggga ccgaagcggt cctcgagctc ctcgcgcagg acgatcaggt cggcgatctc
     2761 cttggcgccc gcgacgcggc ggtggatctc gatcttcgcc gcctcgtagg ggacgtagtc
     2821 gccggggacg aacgcgtcga cgggcaggtc gacgcgcacc ggctccggcg cctcctcggc
     2881 cgagtcgccg gcgagcgccg ccaccgcctc gtcgagcatg ccgacgtaga gctcgaagcc
     2941 gatcgccgcg acgtggccgg actggtcgtc gccgagcagg ttgccggcgc cgcggatctc
     3001 gaggtcgcgc atcgcgatct tgaagccgga gccgagctcg gtgtagtcgg agagggtcgc
     3061 gagccggtcg cccgcctcgc tggtcagcgc ggccgccgag gggtagagca ggtacgcgta
     3121 ggcgcgctcc ttggagcgcc cgacgcggcc cctgatctgg tagagctggg cgaggccgag
     3181 ctcgtcggcg cgctcgacga tcagcgtgtt cgcgttcgag atgtcgaggc ccgactcgac
     3241 gatcgtcgtg cagaccagca cgtccgcctc gccgcgcagg aacgcgagca tgatgccctc
     3301 gagctggccc tcctccatct ggccgtgggc gaccagcacg cgcgccttcg ggcacatcgc
     3361 gcgcacgcgc tcggccgtct cgtcgatcga gtcgacccgg ttgtgcagga agaacgcctg
     3421 ccccctgcgc tcgagctcgc gctcgatcgc cttctggacg aggtcctcgt cgtactcgcc
     3481 gacgtaggtc ttgaccggcc gacggccctc cggcggcgtc tcgatcaccg agatgtcgcg
     3541 caggccggcg agcgacatct gcagcgtgcg cgggatcggc gtcgccgaca gcgacagcac
     3601 gtcgacgcgc aaccgcagct gacgcagcag ctccttctgc ttgacgccga agcgctgctc
     3661 ctcgtcgacg atcagcaggc cgaggtcctt tggccgcacg tcgcgcgaga gcagccggtg
     3721 ggtgccgatc aggatgtcga ccttgccgtc ctcgaagtcc ttcagcgcgg cgcggacctc
     3781 cttgtcggag cgaaagcgcg acacctgctc gatcctgaac gggtagtcgc gcaggcgctc
     3841 ggagaaggtg ccgtggtgct gctgggcgag cacggtcgtc ggcacgagga acatcacctg
     3901 cttggagtcg gccgcggcct tgaaggctgc gcgcagcgcg acctcggtct tgccgtagcc
     3961 gacgtcaccg cagatcaggc ggtccatcgg ccgcgcctcc tccatgtcgg cgcggaccgc
     4021 ctcgatcgcg tcgagctggt c
//
LOCUS       DATABV010000215         9959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1356783, whole genome shotgun sequence.
ACCESSION   DATABV010000215 DATABV010000000
VERSION     DATABV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356783"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VHF88_09885"
     CDS             <1..735
                     /locus_tag="VHF88_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX2162115.1"
                     /translation="ATRATQRAERERAHLPTVAIAGYTNAGKSTLLNALTGAEVGVRD
                     RLFHTLDPTTRSFEIAGRSYLLTDTVGFIRKLPHQLVDAFGATLQEALEADLLLHVAD
                     ASAPEEDLADMLEAVEQVLGEIGADERPRLLALNKVDLLDDERRRELSFRFPQAVQVS
                     AETGAGLDELREAVEARFLSSLRAMELLVPYEEGGRLSELHDLAGEMEREDTADGVRV
                     RARVPVSAAARFERFESNGASPNGTG"
     gene            732..1163
                     /gene="dut"
                     /locus_tag="VHF88_09890"
     CDS             732..1163
                     /gene="dut"
                     /locus_tag="VHF88_09890"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX2162116.1"
                     /translation="MRLRFARLTENAAAPTRAHEDDAGYDLRASEAATLAPGERASIG
                     TGVAVAIPAGHAGLVLPRSGLAARHGIALVNAPGLIDAGYRGEIRVLLLNTDRESGFE
                     VSPGDRIAQLVIVRHEAPELVEVDSLDATARGVGGFGSSGR"
     gene            1216..2946
                     /locus_tag="VHF88_09895"
     CDS             1216..2946
                     /locus_tag="VHF88_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX2162117.1"
                     /translation="MSGATADPIDSTPAGFTPPSQRSGTLKRIGDIIVELGFATREAV
                     ERAVQEARATGSTTGRTLLAGDVVTADQLARAVAERYGVDHVDLDVATIDPAATALVE
                     PSVLRRHRAVPIAFADQRTLIVATSDPANVLAADDLAMMTGYEIRRAVATEDDVELLL
                     GRLTRLDTAVAAVEAEPEAQVIELRESADDAPVVNLVHSIVAEAVERGASDIHFDPES
                     GAMRVRQRIDGVVSDSTTVPKAMVSGLVSRVKIMADLDIAERRLPQDGRIGLTVDGRY
                     VDIRVATLPTVHGESVVMRVLDRGRGMLELDHLGFEPADIVRLRAAISQLHGCVLATG
                     PTGSGKSTTLYAALSEINQPERTLLTIEDPVEYEIDGIKQIQVNPKIGLDFATGLRSM
                     MRADPDVMMVGEIRDRETAQIAIQSALTGHLVLSSLHTTDAPLSVARLIEMGIEPFLI
                     ASGLNVVVAQRLARRLCESCRREVEITSAALAANGLGDHDPFTAYEAGPGCVRCSQTG
                     YRGRVGIYELMEITDEIGELIVEKRPAEAIAAVATTQGMRRLRDNGLAKVRSGGTSMS
                     ELLRVLGATA"
     gene            3155..3248
                     /locus_tag="VHF88_09900"
     tRNA            3155..3248
                     /locus_tag="VHF88_09900"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3192..3194,aa:Val,seq:gac)
     gene            complement(3440..4174)
                     /locus_tag="VHF88_09905"
     CDS             complement(3440..4174)
                     /locus_tag="VHF88_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162118.1"
                     /translation="MTTASPTLRVCALYPELMNIYADRGNIAILRARCEWRGLGFQLA
                     ASSLTEPLAAGDHDLFYIGGGQDRDQIAVAADMAATKRDALHRARDAAAVILAVCGGY
                     QLLGHSYQLGEQELPGVGLVDLTTVREAGPRLIGNCAIEADLGNGPQTIAGFENHGGR
                     TYLGPDERPLGRVLSGHGNNGKDGFEGVHSHNVVGTYLHGPLLPKNVWLADRLIELAL
                     DIELDPLPGDEVESAAHASALRAAGV"
     gene            complement(4171..4917)
                     /locus_tag="VHF88_09910"
     CDS             complement(4171..4917)
                     /locus_tag="VHF88_09910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2162119.1"
                     /translation="MTSARTAADPAAWHDVECGSYSADLALWRELAADRGSSVLDVGC
                     GTGRVALELAAAGHEVVGIDADPALTAALDERAAVAGLPARAIVADARSFTLDRRFPL
                     AILPMQVAQLLGGPKGRARLLATVARHLLPGGLLAIALADPFDAVEPGEARPPLPDVL
                     ETDGWVLSSTPVAVRDEGDAVAIDRIRQAVSPDGDLTEELATVTLDNCTPATLQDEGR
                     AAGFAVQPPRHVPPTADYVGSTVVMLEAPR"
     gene            complement(4914..5477)
                     /locus_tag="VHF88_09915"
     CDS             complement(4914..5477)
                     /locus_tag="VHF88_09915"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX2162120.1"
                     /translation="MSQADLVFGYGSLAHDLLVEPAVGRLYGYRRTFGVAADNSLAIP
                     GYKRFRSPVDGSFPDVYVAFLDLLDDPAGVVNGVLAAVDPATLADLDRRERNYDRVEV
                     TSAIDSAPAGRVWAYIGSPDGRDRFQAGKAAGSLVVQRAYLDRVHDAFCRLGEAEHDH
                     FLATSDLDGIPVADLERVDLPPDDSAP"
     gene            complement(5474..6847)
                     /locus_tag="VHF88_09920"
     CDS             complement(5474..6847)
                     /locus_tag="VHF88_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX2162121.1"
                     /translation="MLRVWRLLARLVRALSRRLGRGGGTTLPGRLLLRVAPDALDRMA
                     ADLDDGAAVISATNGKTTTAAMVAAILERSGERIVHNRAGSNMGWGVATALLDARRGH
                     GQLGLFEVDEAWLPQVARSLHPRSYLLANLFRDQLDRYGELESLAEGWAGLVAEQDGS
                     ARFVLNADDPLVADLGRGRSGVVYFGVDDDTLAIPELQHAADSKHCRNCGTAYVYDAI
                     YLGHLGRYRCPGCDRSRPAPSVTATRVALDGMAGADVTLRTQDGTIDFRLPLPGLYNV
                     YNAAAAAALALELGASLADVEYALAGFGGAFGRVETIAVPHPAGGERPVSILLIKNPA
                     GANEVLRTLTLADAPIDLWLALNDKIADGRDVSWIWDADFELLAGHVRHVTCTGTRAE
                     ELALRLKYAGIDAQTAVDRDLAAALDTAVAAGEPDRPLYALPTYTALLELRDLLSQRG
                     LAERWAG"
     gene            complement(6847..7725)
                     /locus_tag="VHF88_09925"
     CDS             complement(6847..7725)
                     /locus_tag="VHF88_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2162122.1"
                     /translation="MSGRYAVICNPVAGGGRGRKRLPLAVAELERLGAEHRVVETRDI
                     EHARSEARAAIAASETVIAIGGDGLLRPIAAELRDSESALAIVPGGRGNDLARVLGIP
                     TETAAATRIAVEGREWAMDMALLDGEPYLGIASFGFDSDANRIANEARLLRGDLVYLY
                     AALRALVAWRPARFTLTVDGERHELTGFSAGAANSRAYGGGMFAAPHAKLDDGVLEVV
                     ACRDMPRRRFLFTFLPKVFKGSQFSDPRFFARSGTSIELAADRPFTIYADGDPIGDLP
                     ARVEVAPRCLRVIVPR"
     gene            complement(7736..8560)
                     /locus_tag="VHF88_09930"
     CDS             complement(7736..8560)
                     /locus_tag="VHF88_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX2162123.1"
                     /translation="MTLSPLLQAYDQVILDLDGCVWIGDEPVSGSPEAIAALRAAGKR
                     VAFATNNSWHPPEDQVAKLWRHGVQASLGDVVTVGGALQHLLNETRRGRSAFVIGGAS
                     LHANVADAGLRVLNGSDDAERADVVVMGGTDDVTYDDLRVATLAARRSGDLLATARDP
                     TLPMPGGHWPGTGALLAAVETASGVTGQIVGKPQPQLFHTAIDRLGERRTLVVGDRLD
                     SDIAAATAAGLDGALVLSGGMTPDEAAAATSRDDAPQPVAIAADLRALVLADGRQT"
     gene            8605..9312
                     /locus_tag="VHF88_09935"
     CDS             8605..9312
                     /locus_tag="VHF88_09935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2162124.1"
                     /translation="MIRSLTALIALLALLFLAACGDDDSDSGNGSGGGASAQTDTAAQ
                     DDQQCARVDAPKAKPDGGATKPKGKLDPAKTHTVTFQTSCGDFTVTLDVEGAPNTAAS
                     FAALARSGFYDDTVFHRIVPGFVIQGGDPTGTGTGGPGYKTVDKPPANASYTRGVVSM
                     AKTAAEAPGTAGSQFYVVTGADAGLPPDYAIVGEVTDGMDAVMRIEALGDPSGSEQPT
                     RPVVIEKATVSASAAGR"
     gene            complement(9309..9920)
                     /locus_tag="VHF88_09940"
     CDS             complement(9309..9920)
                     /locus_tag="VHF88_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162125.1"
                     /translation="MAKQRQQKMRQVQVGSLAAIQLLEERTDEQLQSESKHRVAAKAL
                     LAARAAERYDAATARKYFNEALSGCHPQERPALRQMMNASMALAERRPDDLKAAVEKL
                     GQAPPSGRQLFMLRLMGLIAPAAGAGTMARVRGILILAAIVIAILAIGFGLAKLIALP
                     FGGVGTFAGLLLGLLIVVAVLGVLALLGRRRQAKAAARRAAGG"
BASE COUNT         1406 a         3494 c         3755 g         1304 t
ORIGIN      
        1 gccacgcgcg cgacgcagcg cgccgagcgc gagcgcgccc acctgccgac ggtcgccatc
       61 gccggctaca cgaacgcagg caagtcgacg ctgctcaacg ccctgaccgg cgccgaggtg
      121 ggtgtgcgcg accgcctctt ccacacgctc gacccgacga cgcggtcgtt cgagatcgcc
      181 ggccgcagct acctgttgac cgacaccgtc ggcttcatcc gcaagcttcc gcatcagctc
      241 gtcgacgcgt tcggcgcgac gctgcaggag gcgctcgagg ccgacctact gcttcacgtc
      301 gccgacgcct cggcaccgga ggaggacctc gccgacatgc tcgaggcggt cgagcaggtg
      361 ctcggcgaga tcggcgccga cgagcggccg cggctgctcg cgctcaacaa ggtcgacctg
      421 ctcgacgacg agcgccggcg cgagctgagc ttccgcttcc cgcaggccgt gcaggtgtcg
      481 gcggagaccg gcgcggggct ggacgagctg cgcgaggcgg tcgaggcgcg cttcctctcc
      541 agcctgcgcg cgatggagct gctcgtgccg tacgaggagg gcggccggct gtcggagctg
      601 cacgacctcg ccggcgagat ggagcgcgag gacaccgccg acggcgtgcg ggtgcgggcg
      661 cgtgtgccgg tcagcgcggc ggcccgcttc gagcgcttcg aaagcaacgg ggcgagcccg
      721 aacgggactg gatgaggctg cgcttcgcgc ggctgaccga gaacgccgcc gcaccaaccc
      781 gcgcccacga ggacgacgcc ggctacgacc tgcgcgccag cgaggcggcg acgctcgcgc
      841 cgggggagcg ggccagcatc ggcaccggcg tcgccgtcgc gatcccggcc ggccacgccg
      901 gcctcgtcct gccacgctcc ggactcgccg cccgccacgg catcgcgctc gtcaacgcgc
      961 ccggcctgat cgacgccggc taccgcggcg agatccgtgt cctgctgctc aacaccgatc
     1021 gcgagtcggg tttcgaggtc tcgcccgggg accggatcgc ccagctcgtc atcgtccgcc
     1081 acgaggcccc cgagctggtc gaggtcgaca gcctcgacgc gaccgcccgc ggcgtgggcg
     1141 gcttcggctc cagcggccgg tgaatccgcc ttcccagcgg ctcaaccctc ggcgcgggct
     1201 gccgatagtc gaaaggtgtc aggcgcgaca gcggacccga tcgactcgac cccggccggg
     1261 ttcacgccgc cgtcgcagcg cagcggcacc ctaaagcgca tcggcgacat catcgtcgag
     1321 ctcggcttcg cgacccgcga ggcggtcgag cgggcggtcc aggaggcccg ggccaccgga
     1381 tcgacgaccg gccgcacgct gctagccggc gacgtcgtga cagccgacca gctcgcgaga
     1441 gcggtcgccg agcgctacgg cgtcgaccac gtcgacctcg acgtcgcgac gatcgacccg
     1501 gcggcgaccg cgctcgtcga gccatcggtc ctgcgccgcc accgcgccgt cccgatcgcc
     1561 ttcgccgacc agcgcacgct gatcgtcgcc acctcagacc ccgcgaacgt cctcgccgcc
     1621 gacgacctcg cgatgatgac cggctacgaa atccgccgtg ccgtcgccac cgaagacgac
     1681 gtcgagctgc tgctcggccg gctcacgcgg ctcgacacgg cggttgccgc cgtcgaggcg
     1741 gagcccgagg cgcaggtgat cgagctgcgc gagtcggctg acgacgcgcc cgtcgtcaac
     1801 ctcgtccact cgatcgtcgc agaggccgtc gagcgcggcg cgtccgacat ccacttcgac
     1861 cccgagagcg gcgcgatgcg cgtccgccag cgcatcgacg gcgtcgtctc cgactcgacg
     1921 accgtcccca aggcgatggt ctcgggcctc gtctcgcgcg tcaagatcat ggccgacctc
     1981 gacatcgccg agcgccgtct gccccaggac ggccgcatcg gcctgaccgt cgacggccgc
     2041 tacgtcgaca tccgcgtcgc gacgctgcca accgtccacg gcgagtccgt tgtcatgcgc
     2101 gtgctcgaca ggggccgcgg gatgctcgag ctcgaccacc tcggcttcga gcctgccgac
     2161 atcgtccgcc tgcgcgccgc gatctcccag ctccacggct gcgtgctcgc caccgggccg
     2221 accggctccg gcaagtcgac gaccctctac gccgccctca gcgagatcaa ccagcccgag
     2281 cgcaccctgc tgacgatcga ggaccctgtc gagtacgaga tcgacggcat caagcagatc
     2341 caggtcaacc cgaagatcgg cctcgacttc gccaccggct tacggtcgat gatgcgcgcc
     2401 gaccccgatg tgatgatggt cggcgagatc cgcgaccgcg agaccgccca aatcgccatc
     2461 cagtccgcgc tcaccggcca cctcgtcctc tcgtcgctgc acaccaccga cgccccgctc
     2521 agcgtcgcgc gcctgatcga gatgggcatc gagcccttcc tgatcgcctc cggcctcaac
     2581 gtcgtcgtcg cccagcgcct cgcccgccgc ctctgcgaga gctgtcgccg cgaggtcgag
     2641 atcacctccg ccgcactcgc cgccaatggc ctcggcgacc acgacccctt caccgcctac
     2701 gaggccgggc cgggctgcgt ccgctgctcc cagaccggct accgcgggcg cgtcggcatc
     2761 tacgagctga tggagatcac cgatgagatc ggcgagctga tcgtcgagaa gcggccagca
     2821 gaggcgatcg ccgccgtcgc caccacacaa ggcatgcgcc gcctgcgcga caacggcctc
     2881 gccaaggtcc gctccggcgg aacctccatg tccgagctgc tgcgcgtgct cggcgccacc
     2941 gcctgagcgg ggcggtacta ccagctaggg ctcgggcgtt ggtggccgcg ggtcctgtct
     3001 ccgattttgg ttcacgtgct cgttcctgcg cgcgcgaacg caaaatcgtc gccacccgcg
     3061 ggtcagcgtg gcagtgaaga ttccgtaacg gttcgcgaca aagcggtaac agcgggctcg
     3121 tcgctgcctg aggctgagct agggtcgccg ggcgcggagg gttggagcag acaggcgcgc
     3181 tgcaggcggc tgacacccgc actgttcggc ctagcggtcg aacccggttc gagtccggcg
     3241 ccctccgccg aataccgcca gatgcggagt cgagactgag gtgagcggtc gcgctaaagt
     3301 ggccactggg tgtcagccgc ctgcggggta gctccccgct tcgacagtcc tcgacccgcc
     3361 tctggcgggt cgttggcttt ctggggacca ggacccgcgg cctgcacggt ctgtccctag
     3421 ccaggagagc cgcgacgctc tagacgccag ccgcacgtag ggcggaggcg tgggcggcgg
     3481 actcgacttc gtcgccgggg agggggtcga gctcgatgtc gagggcgagc tcgatcaggc
     3541 ggtcggcgag ccagacgttc ttgggcagga gggggccgtg gaggtaggtg ccgacgacgt
     3601 tgtgggagtg gacgccctcg aagccgtcct tgccgttgtt gccgtggccg gagaggacgc
     3661 ggccgagagg ccgctcgtcg gggccaaggt aggtgcggcc gccgtggttc tcgaagccgg
     3721 cgatcgtctg ggggccgttg ccgaggtcgg cctcgatggc gcagttgccg atcaggcggg
     3781 ggccggcctc gcggacggtg gtcaggtcga cgaggccgac gcccgggagc tcctgctcgc
     3841 cgagctggta gctgtggccg agcagctggt agccgccgca gacggcgagg atgacagcgg
     3901 cggcgtcgcg cgcgcggtgg agggcgtcgc gcttggtggc tgccatgtcg gcggcgacgg
     3961 cgatctggtc gcggtcctgg ccgccgccga tgtagaagag gtcgtggtcg ccggcggcga
     4021 gcggctcggt cagggagctg gcggcgagct ggaagccgag gccgcgccat tcgcagcggg
     4081 cacggaggat cgcgatgttg ccgcgatctg cgtagatgtt catcagctcg gggtagagcg
     4141 cgcagacgcg gagggtgggg gaggcggtgg tcatcgcggc gcctccagca tgacgacggt
     4201 gctgccgacg tagtcggctg tcggcggcac gtggcgcggt ggctggacgg cgaagccggc
     4261 ggcgcggccc tcgtcctgca gggtggccgg ggtgcagttg tcgagggtga cggtggcgag
     4321 ctcctcggtg aggtcgccgt cgggggagac ggcttggcgg atgcggtcga tcgcgacggc
     4381 gtcgccctcg tcgcggacgg cgaccggggt cgaggagagc acccagccgt cggtctcgag
     4441 gacgtcgggc agcggtgggc gcgcctcgcc gggctcgacg gcgtcgaagg ggtcggcgag
     4501 tgcgatcgcg agcaggccgc cgggcaggag gtggcgggcg acggtcgcga gcaaccgggc
     4561 gcggcccttg gggccgccga gcagctgggc gacctgcatc ggcaggatcg cgagcgggaa
     4621 gcgacggtcg agggtgaagc tgcgcgcgtc ggcgacgatc gcgcgagccg gcaggccggc
     4681 cacggcggcg cgctcgtcga gcgcggcggt gagcgcgggg tcggcgtcga tgccgacgac
     4741 ctcgtgcccg gccgccgcga gctcgagcgc aacgcggccg gtgccacagc cgacgtcgag
     4801 cacggagctg ccgcggtcgg cggcgagctc gcgccagagc gcgaggtcgg cgctgtagga
     4861 gccgcactcg acgtcgtgcc aggcggcggg atcggctgcc gtgcgtgcgg aggtcacggc
     4921 gcgctgtcgt cgggcggcag gtcgacgcgc tcgaggtcgg cgacggggat gccgtcgagg
     4981 tcggaagtgg cgaggaagtg gtcgtgctcg gcctctccga ggcggcagaa ggcgtcgtgc
     5041 acccggtcga ggtaggcgcg ctggacgacg agcgagccgg cggccttgcc ggcctgaaag
     5101 cggtcacggc cgtcgggcga gcctatgtag gcccagaccc ggccggctgg cgcactgtcg
     5161 atcgcgctgg tcacctcgac gcgatcgtag ttgcgctcgc gccggtcgag atcggcaagc
     5221 gtggcggggt cgactgccgc caggacgccg ttgacgaccc cggccgggtc atcgagcagg
     5281 tcgaggaacg cgacatagac gtcggggaag gagccgtcga cgggcgaccg gaatcgcttg
     5341 tagccgggaa tcgccagcga gttgtcggcc gcgacgccga aggtccgccg gtaaccgtag
     5401 agacggccga cggcgggctc gacgagcagg tcgtgggcta gcgacccgta gccgaagacg
     5461 agatcggcct ggctcaaccg gcccatcgct cggcgaggcc gcgctggctg agcaggtcgc
     5521 gcagctcgag cagcgcggtg taggtcggga gcgcgtagag cggacgatcg ggctcgccgg
     5581 cagccacggc cgtgtcgagg gcggcggcga gatcgcggtc gacggccgtc tgggcgtcga
     5641 tcccggcgta cttgagccgc agcgcgagct cctcggcgcg agttccggtg caggtgacgt
     5701 ggcgcacgtg cccggcgagc agctcgaagt cggcgtccca gatccaggag acgtcgcggc
     5761 cgtcggcgat cttgtcgttg agggcgagcc agaggtcgat cggcgcgtcg gcgagcgtca
     5821 gcgtgcgcag cacctcgttg gctccggcgg ggttcttgat cagcaagatc gacacgggcc
     5881 gctcgccgcc cgcggggtgg gggaccgcga tcgtctcgac gcggccgaag gccccgccga
     5941 agccggcgag cgcgtactcg acgtcggcga gcgaagcgcc gagctcgagc gccagcgcgg
     6001 cggctgcggc ggcgttgtag acgttgtaga ggccgggcag cggtaggcga aagtcgatcg
     6061 tcccgtcctg cgtgcgcagc gtgacatcgg cgccggccat cccgtcgagc gcgacccgcg
     6121 tggctgtgac gctcggcgcg gggcgactgc ggtcgcagcc ggggcagcgg taacggccga
     6181 ggtggccgag gtagatggcg tcgtagacgt acgccgtgcc gcagttgcgg cagtgcttgg
     6241 agtcggcggc gtgctggagc tccgggatcg ccagcgtgtc gtcgtcgacg ccgaagtaga
     6301 cgacgccgct gcggccgcgg ccgaggtcgg cgacgagcgg gtcgtcagcg ttgagcacga
     6361 agcgcgcgct gccgtcctgc tcggccacca gtccggccca gccctcggcg agcgactcga
     6421 gctcgccgta gcggtcgagc tggtcgcgaa agaggttcgc cagcaggtag ctgcgcgggt
     6481 gcaacgagcg cgcgacctgg ggcagccacg cctcgtcgac ctcgaacagg ccgagctgac
     6541 cgtggccgcg acgcgcgtcg agcagcgcgg tcgcgacgcc ccagcccatg ttcgagccgg
     6601 cgcggttgtg gacgatccgc tccccggagc gctcgaggat cgcggcgacc atcgcggcgg
     6661 tggtcgtctt gccgttggtg gcggagatca ccgcggcgcc gtcgtcgagg tcagcggcca
     6721 tccggtcgag cgcgtcgggc gcgacgcgca gcagcagtcg gcccggcagc gtcgtgccgc
     6781 cgccgcggcc cagccggcgg ctcagcgcgc ggacgaggcg ggcgagcagg cgccagaccc
     6841 ggagcatcag cgcgggacga tcacgcgcag gcagcgcggg gcgacctcga cgcgtgcggg
     6901 caagtcgccg atcgggtcgc cgtcggcgta gatcgtgaag gggcggtcgg cggccagctc
     6961 gatcgacgtc ccgctcctgg cgaagaagcg gggatcggag aactggctgc ccttgaagac
     7021 cttggggagg aaggtgaaga ggaagcgccg gcgcggcatg tcgcgacacg cgaccacctc
     7081 cagcacgccg tcgtcgagct tcgcgtgcgg cgcggcgaac atcccgccgc cgtaggcgcg
     7141 cgagttggcg gcgccggccg agaagccggt cagctcgtgg cgctcgccgt cgaccgtcag
     7201 ggtgaagcgc gccggtcgcc aggcgaccag cgcccgcagt gccgcgtaga ggtagacgag
     7261 gtcgccgcgc agcaggcgtg cctcgttggc gatccggttg gcgtccgagt cgaagccgaa
     7321 gctggcgatg ccgaggtacg gctcaccgtc gagcagcgcc atgtccatcg cccattcgcg
     7381 gccctcgacg gcgatccgcg tcgccgcggc cgtctcggtc gggattccga gcacccgggc
     7441 caggtcgttg ccgcgaccgc cgggcactat cgccagcgcg gactcactgt cgcgcagctc
     7501 ggcggcgatc gggcgcagca ggccgtcgcc gccgatcgcg atcaccgtct cgctcgcggc
     7561 gatggccgca cgcgcctcgc tgcgggcgtg ctcgatgtcg cgcgtctcga ccacccggtg
     7621 ctcggcgccg agccgctcga gctcggcgac ggccagcggg aggcgcttgc ggccgcggcc
     7681 tccacccgcc acggggttgc agatgacggc atagcgcccg ctcacgcgcg ccagtctatg
     7741 tctggcgtcc gtcagccagc acgagcgcac gcaggtcggc ggcgatcgct actggctgcg
     7801 gtgcgtcgtc gcgcgatgtg gccgcggccg cctcgtcggg ggtcatcccg ccgctcagga
     7861 cgagcgcgcc gtcgaggccg gcggcggtcg ccgcggcgat gtcgctgtcg aggcgatcgc
     7921 cgacgacgag cgtgcggcgc tcgccgagcc ggtcgatcgc cgtgtggaag agctgaggct
     7981 ggggcttgcc gacgatctga ccggtcacgc cggaggccgt ctcgacggcg gccagcagcg
     8041 cgccggtgcc gggccagtgg ccgcccggca tcggcagcgt gggatcgcgc gccgtggcga
     8101 gcagatcccc gctgcgccgc gcggccagcg tcgcgacgcg caggtcgtcg taggtgacgt
     8161 cgtcggtgcc gcccatcacg acgacgtccg cgcgctcggc gtcgtcgctg ccgttgagca
     8221 cccgcaggcc ggcgtcggcg acgttggcgt gcagcgacgc gccgccgatc acgaaggcgc
     8281 tccggccgcg acgggtctcg ttgagcaggt gctgcagcgc gccgccgacg gtgacgacgt
     8341 cgccgagcga ggcctggacg ccatggcgcc agagcttggc gacctggtcc tctgggggat
     8401 gccaggagtt gttcgtcgcg aacgcgacgc gcttgccggc ggcgcgcagc gcggcgatcg
     8461 cctccggtga gccggaaacc ggctcgtcac cgatccagac gcagccatcg aggtcgagga
     8521 tgacctggtc gtaggcctgg aggagggggg agagggtcac cttcggcggc gaactctaga
     8581 gctgcgtgga ctagtctgcg cgccgtgatc cgctcgctga cagcactcat cgccctgctc
     8641 gccctgctct tcctcgccgc ctgcggcgac gacgactccg actccggcaa cggctccggc
     8701 ggcggcgcca gcgctcaaac cgacacggcg gcgcaggacg accagcagtg cgcgcgcgtc
     8761 gacgcgccga aggcgaagcc cgacggcggc gccaccaagc ccaagggcaa gctcgacccg
     8821 gcgaagaccc acaccgtgac gttccagacg agctgcggcg acttcacagt gacgctcgac
     8881 gtcgagggag cgcccaacac cgccgcctcc ttcgcggcgc tcgcgcgcag cggcttctac
     8941 gacgacacgg tcttccatcg catcgtcccc ggcttcgtga tccagggtgg cgacccgacc
     9001 ggcaccggga ccggcgggcc cggctacaag accgtcgaca agccgcccgc gaacgccagc
     9061 tacacgcgcg gagtggtgtc gatggccaag acggccgcgg aggcacccgg gacggccggc
     9121 agtcagttct acgtggtgac cggagccgac gccggcctgc cgccggacta cgcgatcgtc
     9181 ggcgaggtca ccgatgggat ggacgcggtg atgcggatcg aggcgctcgg cgacccgagc
     9241 ggcagcgagc agccgacgcg gccggtcgtg atcgagaagg cgacggtcag cgctagcgcg
     9301 gcggggcgct agccgcccgc ggcgcgcctg gccgcggcct tcgcctgacg gcgccggccg
     9361 agcagcgcca gcacgccgag caccgcgacg acgatcagca gcccgagcag cagaccggcg
     9421 aaggttccga cgccgccgaa cggcagcgcg atcagcttgg cgaggccgaa gccgatcgcg
     9481 aggattgcga tcacgatcgc ggcgaggatc aggatgccgc gcacgcgggc catcgtgccg
     9541 gcgccggcag ccggcgcgat cagacccatc agacgcagca tgaacagctg acgcccggat
     9601 gggggggcct gcccgagctt ctcgaccgcc gccttgaggt cgtcgggccg gcgctcggcg
     9661 agcgccatcg acgcgttcat catctgacgc agcgccgggc gctcctgcgg atggcagccg
     9721 gacagcgcct cgttgaagta cttgcgggcg gtggcggcgt cgtagcgctc ggcggcgcgg
     9781 gcggcgagca gcgccttggc ggcgacgcgg tgcttcgact cgctctgcag ctgctcgtcg
     9841 gtccgctcct cgagcagctg gatcgccgcg agcgagccga cctggacctg ccgcatcttc
     9901 tgctgcctct gcttggccat ccccgcgagg atatccactg ggggctcacg ccccagacc
//
LOCUS       DATABV010000216        16346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2159739, whole genome shotgun sequence.
ACCESSION   DATABV010000216 DATABV010000000
VERSION     DATABV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16346
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159739"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VHF88_09945"
     CDS             complement(<1..220)
                     /locus_tag="VHF88_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2162126.1"
                     /translation="MSVNTSLVGSAIAKWGTEEQKQRYIPGICSGEALGCFGLTEPDT
                     GSDAANLSTRAERVDGGWRITGQKQWISL"
     gene            207..1109
                     /locus_tag="VHF88_09950"
     CDS             207..1109
                     /locus_tag="VHF88_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2162127.1"
                     /translation="MFTDTTVRADESAIAKWGSEEQKQRFIPGICSGEALGCFGLTEP
                     DTGSDAANLSTRAERIDGGWRISGQKQWISLGNHAAFALIFAQTDPQLGHRGLGAFLV
                     PTDSPGFSASEIHGKLGLHASDTAELALDGVEVGDDALLGEVGDGFKVAMTALDSGRF
                     SVSAGCVGICQGCVAASTAYAKEREQFGRPIAAFQLVQAMIADMVVDTHAARGLVWKA
                     AWLKDSGQPNTLETSVAKLFSTEAAIRSANTAIQVHGGSGYVDDHPVERYLRDARVTT
                     LYEGTSQIQKLIIGRLTTGINAMT"
     gene            complement(1106..3895)
                     /locus_tag="VHF88_09955"
     CDS             complement(1106..3895)
                     /locus_tag="VHF88_09955"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX2162128.1"
                     /translation="MGNPNSFGARSTLQVGGATHEIFRLDALQSQFDVARLPYSLKIL
                     LENLLRNEDGEAVTREDIENLARWVATDEPSQEIAYSPSRVLMQDFTGVPAIVDLAAM
                     RDAMEELGGDPTRINPLVPVELVIDHSIQVDSFAERLAFQKNAEREYERNRERYAFLR
                     WGQGAFDNFAVVPPNTGICHQVNLEYLSRVVTARDGQAFPDTLVGTDSHTTMVNGLGV
                     LGWGVGGIEAEAAMLGQPVSMLLPQVVGFRLEGALPEGATATDLVLTVTERLRQHGVV
                     GRFVEFFGPGLANLPLADRATIGNMSPEFGSTCAIFPIDAETLRYLEFSGRPAEQIEL
                     VEAYAREQGLFHDADSEDAVYSDTLSLDLSTVEPSLAGPKRPQDRIALSQAASDFLAE
                     LADQANGKDGDHGGAAGSRMSFPASDPPAAMAGQNGEDDGEPAHAHGAVAEAPAKRST
                     AVSLGGQEFDLEDGAVVIAAITSCTNTSNPSVMVGAGLLAQRAVERGLTRKPWVKTSL
                     APGSKVVTEYLDRAGLTAPLEQLGFNLVGYGCTTCIGNSGPLPQEVSAAIEAEDLTVC
                     SVLSGNRNFEGRIHPEVKMNYLASPPLCVAYALAGRMDLDLLSDPLGEDRDGDPVYLR
                     DLWPSQAEIHAAVEAAVQSDMFRKSYGEVYEGDENWRALDTPEGDRYAWDDGSTYVKR
                     PPYFEAMPAEPPEGFDEIRGARVLALLGDSVTTDHISPAGAIKRDGPAGRYLLDHGVE
                     QRDFNSYGSRRGNHEVMMRGTFANVRLRNRLAPGTEGGVTVKDGEQTTIYDAAMAYQA
                     EGVPLCVLAGKEYGSGSSRDWAAKGPRLQGVRFAIAESYERIHRSNLVGMGVLPLQFP
                     DGESVESLGLTGFETFDLAPLEEGARTLEVTATPDEGAPIVFEARVRIDTPNEWLYYR
                     HGGILHFVLRQLRQR"
     gene            3924..4559
                     /locus_tag="VHF88_09960"
     CDS             3924..4559
                     /locus_tag="VHF88_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162129.1"
                     /translation="MRPSLRRSLIAAGLVSIAALLPAAPAAAQSPVVCPATFHVLHDD
                     SIGDLELPAGNYQVTVRGGLSCPASSEWLARFLEDFNGVLPRPWRLDSDSASFSRGAS
                     AVGFGVARVATPSGGGSGGRHPLSGRLCPGTFRVLNNDRIGSLRLPRGNYTITALSLN
                     RPTCSRASRLFTRFLNDFTGDLPGRWSLDPQTATFRRGAASFGFRVKPVSD"
     gene            4552..5739
                     /locus_tag="VHF88_09965"
     CDS             4552..5739
                     /locus_tag="VHF88_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HEX2162130.1"
                     /translation="MTSFEAYERACAGLDAPFALVDLDAMWHNAASMLARAAGKPIRV
                     ASKSVRCRELLNRILGRDPGFRGLMTFTLAETLWLRGQGFGDLLLAYPTADRASLAEL
                     ARVEGDDRPIVMVDSAEQLDWIARVAPNPAAPLRLCVEFDTGYRLPRGLATIGPKRSP
                     IRTPAQAAALAREIDRRPQLELAGLMGYEGHIAGVGDRPLRKPLQGAAIRAMQRASIA
                     EIAERRAAIVAAVRDVVPVELVNGGGTGSLQTTAAEGAVTEVTAGSGFYASTLFDRYS
                     AFDQRPAAMFALPVVRRPRADTVTLLGGGYVASGPAGKDRLPEPFLPAGLSLDALEGA
                     GEVQTPVRGAAARSLRIGDNVYLRHAKAGELCERFDTLHLLEGGQIVDEVPTYRGEGH
                     AFL"
     gene            5726..7486
                     /locus_tag="VHF88_09970"
     CDS             5726..7486
                     /locus_tag="VHF88_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2162131.1"
                     /translation="MRSSSRLALLALTLVALLAPAAADAKPNIVFIMTDDQTDESTAV
                     QRNVGLIKGDGTTFTETIASYPLCCPSRATYLSGQYSHNHGVIHNAGPFGGYIRFDNS
                     NALPLWLQQAGYRTMHVGRYLNGYGVQNPNIAEVPPGWNDWVSGVGEQVFNYERWQTN
                     ENGQIFNRPGPDHPNEHQTDFYGRRAAELIDQAAPSAQPFYLSLTFPAPHSGFPLEPD
                     DPPFLPTPYPAPRHRNAFAGTPLPRPPNFDEADVSRKPQIVSDRPRLSPEWVAAIQEN
                     YQQELESLLSVDDAVGAVLNALQRSGELDDTLLIYTSDNGFFHGEHRLPSEKILPYDE
                     AARIPLIMRGPGVPRGVELDNLVSNIDWAPTIVDAADARAGRLMDGTSLFETIDDPRR
                     LPGREIVLENGTGANGIPMFRALRNERYLWIEHKFSGEYELYDLVKDPYQLRNLEDLD
                     SYAEIRAALAKRLRRLQRCRGARACGASRPKLRLTTRQEQPPEPKRERPRGEAQATAS
                     CVGRDLRLGIAGRELGEVVRVDYYRGKRKLRATRRTPFEVRVRRRALPRGRKLTLRAL
                     VTTVDGRKATYDRRVQTCRR"
     gene            7483..9114
                     /locus_tag="VHF88_09975"
     CDS             7483..9114
                     /locus_tag="VHF88_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX2162132.1"
                     /translation="MSDGFDYDWLVIGSGFGGSVSALRLAEKGYSVGVLECGRRFEDG
                     DLPSSTWDVRRYSWRPRLGLRGIFRITNFKDVTVISGCGVGGGSLGYANTLYVPPRRF
                     FDDPQWSDLADWHAELSPHYAEAERMLGVVEYDQDDPAGDLLREFGEELGVADTYGRA
                     RVGIFLGEPGKTVPDPYFGGEGPDRTGCTRCGRCMVGCPHGAKNSLIKNYLWLAERRG
                     AKVVAEREVVDVLPLGAADGSEGYAVISERPGAWRRRDRRLHTARGVVLAAGPLGTNK
                     LLANCRHRGSLPRLSRRLGDLVRTNSEAILAVTVPDDSVDYTKRVAITSSIYPDPDTH
                     VETVVYGHAGGVTKLFNTLLVGDGTRVTRPLKLLGTLLRHPGRMLKLLAAPGWSRRTI
                     ILLVMQTLDNAIALRPRPTRDGGVRLQTEQDPQRPIPTFIPVANQAAEWFAERTGGIA
                     QSSIPEAALNIPMTAHILGGAVIGADPESGVVDRDQRVFGYENLLVCDGAAVPANPGV
                     NPSLTITALAEHAMSRIPAKDGPLARTAAEPAASA"
     gene            complement(9133..10158)
                     /gene="trxB"
                     /locus_tag="VHF88_09980"
     CDS             complement(9133..10158)
                     /gene="trxB"
                     /locus_tag="VHF88_09980"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2162133.1"
                     /translation="MTATDGAKQIEDVIIVGSGPAGYTAALYTARADLDPLVIEGFQW
                     GGLLQQTTDVENYPGYPEGVMGPEMMQQFRAQAERFGTRFVTDDATRLELSDGGLQRV
                     WVGDQEYRGRSVVLAMGADPRKLGVPGEEELAARGVSYCATCDAAFYRGKRTIIVGGG
                     DTAMEEATFLAKFAADVKVVHRREEFRASAIMLDRARETENIELVTPFVVERFEAGES
                     GSLARAVLSHSEGGEDRVLEIDGAFIAIGHSPNSQIVADQVEVDENGYVKVEGRSSRT
                     GLGGVFAAGDLIDHTYRQAITAAGSGCAAALDAESYLRDTPIDPEAHWEPDPDRIEAA
                     AERAAAG"
     gene            complement(10155..11306)
                     /locus_tag="VHF88_09985"
     CDS             complement(10155..11306)
                     /locus_tag="VHF88_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2162134.1"
                     /translation="MHDVEVFEEDVDEDLRVSGMGAVLLDRRRLAFGVAFVLALIAAI
                     YVLFPRVVGLNGEIAQISHAAWYWVAIAIAFNVAAFGAYVALFRGVLGGNASAGVRRR
                     LDAGASYQITMAGLAATRIFSAAGAGGIVLTYWALRKAGMPRRRSACRMVAFLVLMYS
                     VYGIALVVFGVLLRTGVLPGDAPVAGTIVPAGLAGGAMLVAGLIALIPGDLERRLSSF
                     RGRGRVARVAAKLAPVPATAATGLRTAVAYVRHPRRGALAVCGAIGFWAANIGVLWAS
                     FEAFGGSVPFGVLVQGFFVGMAANLIPSPAGGVGSVDAGMIGAFVLFGIPAGIVFPAV
                     LTYRVIAFWLPIPPGIVAFFQLRRTVAGWDAGRADYTSKSKVKAEAQAQ"
     gene            complement(11306..12220)
                     /locus_tag="VHF88_09990"
     CDS             complement(11306..12220)
                     /locus_tag="VHF88_09990"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2162135.1"
                     /translation="MRSSRALRTLAWGAVAVGVATPLLRHRLRLRPPVVTALSFQAPI
                     ALALATPRTRLRDAGIYALQMWAYYAHYDMPDDDPDALLRRTRVDYPIRIDRLIGCGE
                     TPTTRLQRAFGTPGEVRRHDLALAMVHWAWFIVPHGTVAFLLLRHHDRFVRGAAMTAA
                     TFDLGLVVYWAIPTAPPWYAAESGRMAPVRRIMVEAGEHFWKRFWRPLYDGLEGNPFA
                     AMPSLHFGTSVMAARVLSDVGRGPGALAWGYALTLGFGLVYLGEHYVVDLLAGYALAE
                     AVRRGTPLAAPAVLRGARKIQRLEPGAT"
     gene            complement(12240..12890)
                     /locus_tag="VHF88_09995"
     CDS             complement(12240..12890)
                     /locus_tag="VHF88_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2162136.1"
                     /translation="MLIDSVVWLGHAGFRVEVKGATVYIDPYRVPPGAPPGDAVLITH
                     GHYDHYSPRDVEALSHDRTWLIAPAAVAERARGRVASIAPGETIELDHLRGIDVTAVA
                     AYNTSKRDSDGNPFHPRTAGWVGFDLNVRGERLYHAGDTDVIPEMDAVTGVDVALLPV
                     CGTYVMTVDEAVEAARRIQPRVAVPSHWGSHLGSRADAEAFAQRAPVDVRLFRPPA"
     gene            12951..13745
                     /locus_tag="VHF88_10000"
     CDS             12951..13745
                     /locus_tag="VHF88_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2162137.1"
                     /translation="MSYETVKWEHEGGVGRIVLDRPETLNAWNTQFGHDLKAVVAEAA
                     DDESVRAVLLTGAGRAFSSGADLKAGFEEAADDGMPDIRGRLHDLFHPSLVGLRTMPK
                     PVVVAVNGPAAGIGVSLALAGDLVLAAESAFFQLAFANIGLMPDGGSTLFVPARVGKA
                     RAFEMALLAERIPAAQALDWGLVNAVHPDGDLIDAASALAERLASGPTRAYASAKQAL
                     NATIYPNLDEQLELEAELQHALGRSKDFMEGGVAFVEKRTPEFTGR"
     gene            13797..14669
                     /gene="coxB"
                     /locus_tag="VHF88_10005"
     CDS             13797..14669
                     /gene="coxB"
                     /locus_tag="VHF88_10005"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX2162138.1"
                     /translation="MPVVRPPHLRRALLALAAGVLLSLLIAPPATADLFTPESGGSPN
                     ADSIDSLYKVIFYVGVVIFLIVEGTLIWALVKYRARRGGPQEGAQIHGNTPLELGWTL
                     GAATIVLVLAVVTFLYLGDIKNPPASGPDGLARGVEVASIDQPEPPKGSSRALTIDVN
                     GQQFLWRYDYPGGAFSYYEMVVPTDTTVVLNLTSSDVIHSWWIPKLGGKMDTVPGYVN
                     KAWFKISEPGVYIGQCAELCGSGHADMRARVRAVSPEQYTAFLERQKADLKASQEALA
                     EQRRQRVEAENEIQ"
     gene            14686..>16346
                     /gene="ctaD"
                     /locus_tag="VHF88_10010"
     CDS             14686..>16346
                     /gene="ctaD"
                     /locus_tag="VHF88_10010"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX2162139.1"
                     /translation="MATPDPATPTPAGTAALRPEVVMHGLSPEPKGWLAWLTTTDHKR
                     IGIMYFVATFIFFILGGVEALIMRAQLIQPDSTLIAPETYNGLVSMHGTTMIFLFIVP
                     VMAAFGNYFVPLMIGARDMAFPRLNALSFWLLAFGGIAFYGSLFFEPPQAGWTSYTPL
                     SDDAYMPGGGIDAWIFLVHLTGLSSLVGAINFVATIHNMRAPGMSWGRMPLFVWSILV
                     YSYLLIAALPAIAAAVTMLLADRHFGTAFFDPTGGGDPMLWQHLFWFFGHPEVYIMIL
                     PAFGIISEVLPVFARKPIFGYKAIAASTVAIAFLGLLVWAHHMFTTPTSTVVLIFFML
                     SSFAIAVPTGIKIFNWIATLWKGSIVFKTPLLFAAALPALFVIGGISGVILAVFPVDW
                     QLHDTYFVVAHLHYVLFGGSVFGIFAGIYYWFPKMTGRMMGEGLGKLSFWVMFVGFNM
                     TFLVQHSAGLSGMPRRVYDYSGDLGVTDYNLISTIGSFLLGIGVLLSVINALRSYRHG
                     KVAGNDPWKGNTLEWFVQSPPPVNNFDVVPRVRSVEPMKDIRREVA"
BASE COUNT         2376 a         5994 c         5582 g         2394 t
ORIGIN      
        1 cgagcgagat ccactgcttc tggccggtga tccgccagcc gccgtcgacc cgctccgcgc
       61 gggtgctgag gttggccgcg tccgagcccg tgtcgggctc ggtcagaccg aagcagccga
      121 gcgcctcgcc cgagcagatc ccggggatgt accgctgctt ctgctcctcg gtgccccact
      181 tggcgatcgc cgagccgacc agcgaggtgt ttaccgacac gaccgtgcgc gccgacgagt
      241 cggcgatcgc caagtggggt agcgaggagc agaagcagcg cttcatcccg ggcatctgct
      301 cgggcgaggc gctgggctgc ttcgggctca ccgagccgga caccggctcg gacgccgcga
      361 acctcagcac gcgcgccgag cggatcgacg gcggctggcg gatcagcggc cagaagcagt
      421 ggatctcgct cggcaaccac gccgcgttcg cgctgatctt cgcccagacc gatccgcagc
      481 tcgggcaccg cggtctgggc gccttcctcg tcccgaccga ctcgcccggg ttctcggcgt
      541 cggagatcca cggcaagctc ggcctgcacg cctcggacac cgccgagctg gcgctcgacg
      601 gcgtcgaggt cggcgacgac gcgctgctcg gcgaggtcgg cgacggcttc aaggtcgcga
      661 tgaccgcgct cgactcaggt cgcttcagcg tctcggccgg ctgcgtcggc atctgccagg
      721 gttgcgtcgc cgcctcgacg gcctacgcca aggagcgcga gcagttcggc cgcccgatcg
      781 ccgccttcca gctcgtccag gcgatgatcg ccgacatggt cgtcgacacc cacgccgccc
      841 gcgggctcgt ctggaaggcc gcctggctca aggacagcgg ccagccgaac acgctcgaga
      901 cgtcggtcgc caagctcttc tcgaccgagg ccgcgatccg cagcgccaac accgcaatcc
      961 aggttcacgg cggctcgggc tacgtcgacg accaccccgt cgagcgctac ctgcgcgacg
     1021 cccgcgtgac caccctctac gagggcacgt cgcagatcca gaagctgatc atcggtcgct
     1081 tgacgaccgg catcaacgcg atgacctagc gctggcggag ctgacgcagc acgaagtgca
     1141 ggatgccgcc gtggcggtag tagagccact cgttcggcgt gtcgatccgc acgcgggcct
     1201 cgaagacgat cggagcgccc tcgtccggcg tcgcggtcac ttcgagcgtc cgcgcaccct
     1261 cctccagcgg cgccaggtcg aacgtctcga agccggtcag gccgagcgac tcgaccgact
     1321 cgccgtcggg gaactggagc ggcagcacgc ccatcccgac gaggttcgag cggtggatcc
     1381 gctcgtagct ctcggcgatc gcgaaccgga cgccctgcag gcgcggcccc ttcgcggccc
     1441 agtcgcgcga cgagccggac ccgtactcct tgccggccag cacgcagagc ggaactccct
     1501 cggcctggta ggccatcgcc gcgtcgtaga tcgtcgtctg ctcgccgtcc ttgacggtga
     1561 cgccaccctc ggtgcccggg gcgagccggt tgcgcagccg tacgttggcg aacgtgccgc
     1621 gcatcatcac ctcgtgattg ccgcggcgcg agccgtagga gttgaagtcg cgctgctcga
     1681 ccccatggtc gagcaggtac cggccagcgg ggccgtcgcg cttgatcgcg ccggccggcg
     1741 agatgtggtc ggtcgtgacg ctgtcgccga gcagcgccaa cacgcgcgcg ccgcggatct
     1801 cgtcgaagcc ctcgggcggc tcggcgggca tcgcctcgaa gtagggcggg cgcttgacgt
     1861 aggtcgagcc gtcgtcccag gcgtagcggt cgccctccgg cgtgtcgagc gcgcgccagt
     1921 tctcgtcgcc ctcatagacc tcgccgtagc tcttgcggaa catgtccgac tggaccgcgg
     1981 cctcgactgc cgcgtggatc tccgcctgcg acggccagag gtcgcgcagg tagacggggt
     2041 cgccgtcgcg gtcctcgccg agcggatcgc tcagcaggtc gaggtccatc cggccggcga
     2101 gcgcgtaggc gacgcagagc ggcggcgagg cgaggtagtt catcttgacc tcggggtgga
     2161 tccggccctc gaagttgcga ttgcccgaca gcaccgagca gacggtcagg tcctcggcct
     2221 cgatcgccgc cgacacctcc tgcggcagcg ggcctgagtt gccgatgcag gtcgtgcagc
     2281 cgtagccgac gaggttgaat ccgagctgct cgagcggcgc ggtcagcccg gcgcgatcga
     2341 ggtactcggt gacgaccttc gagcccgggg cgaggctcgt cttgacccac ggcttgcgcg
     2401 tcaggccgcg ctcgacggcc cgctgggcga gcagtcccgc accgaccatc acggacgggt
     2461 tcgacgtatt cgtgcagctg gtgatcgcgg cgatcacgac cgcgccgtcc tcgaggtcga
     2521 actcctgacc gccgaggctc acggccgtgg agcgcttcgc cggcgcctcg gcgacggcgc
     2581 cgtgggcatg cgccggctcg ccgtcgtcct cgccgttctg gcccgccatc gccgccggcg
     2641 ggtcgctcgc ggggaaggac atccgcgagc ccgccgcgcc cccatggtcg ccgtccttgc
     2701 cgttggcctg gtcggcgagc tcggcgagga agtcgctcgc cgcttgcgac agcgcgatcc
     2761 ggtcctgcgg ccgcttcggg cccgcgaggc tcggctcgac cgtgctgagg tcgagcgaca
     2821 gcgtgtcgga gtagaccgcg tcctcgctgt cggcgtcgtg gaacaggccc tgctcgcgcg
     2881 cgtaggcctc gacgagctcg atctgctcgg ccggccgtcc ggagaactcg aggtagcgca
     2941 gcgtctcggc gtcgatcggg aagatcgcgc aggtcgagcc gaactccggc gacatgttgc
     3001 cgatcgtcgc gcggtcggcg agcggcaggt tcgccaggcc cggcccgaag aactcgacga
     3061 agcgaccgac gacgccgtgc tggcgcagcc gctcggtgac tgtcagcacg aggtcggtcg
     3121 ccgtcgcgcc ctcgggcagc gcgccctcga gccggaagcc gacgacctgg ggcagcagca
     3181 tcgagaccgg ctggccgagc atcgcggcct ccgcctcgat cccgccgacg ccccagccga
     3241 ggacgccgag gccgttgacc atcgtcgtgt gcgagtcggt cccgacgagc gtgtcgggaa
     3301 acgcctggcc gtcgcgggcc gtcacgacgc gcgacaggta ctccaggttg acctggtggc
     3361 agatgccggt gttcggcggc acgaccgcga agttgtcgaa cgctccctgg ccccagcgca
     3421 ggaaggcgta gcgctcgcgg ttgcgctcgt actcgcgctc ggcgttcttc tggaaagcga
     3481 gccgctcggc gaagctgtcg acttggatcg agtggtcgat caccagctcg acgggcacga
     3541 gcgggttgat ccgcgtcgga tcgccgccga gctcctccat cgcgtcgcgc atcgccgcga
     3601 ggtcgacgat cgccgggacg ccggtgaagt cctgcatcag gacgcggctc ggcgagtagg
     3661 cgatctcctg cgacggctcg tcggtcgcga cccagcgggc gagattctcg atgtcctcgc
     3721 gggtgactgc ctcgccgtcc tcgttgcgca gcaggttctc gagcaggatc ttcagcgagt
     3781 acggcagccg agcgacgtcg aactgcgatt gcagcgcgtc gaggcggaag atctcgtgtg
     3841 tcgcgccgcc gacctggagg gtcgagcggg cgccgaagct gttcggattg cccacgcctg
     3901 aaccgtacct gtaccttgcc ggcatgcgcc catccctccg ccgttccctg atcgccgccg
     3961 gcctcgtctc gatcgccgcg ctgctgcctg ccgcgccggc cgcggcccag tcgccggtgg
     4021 tctgcccggc gaccttccac gtgctgcacg acgacagcat cggcgacctc gagctgccgg
     4081 ccggcaacta ccaggtcacc gtccgcggcg ggctctcctg cccggcttcg agcgagtggc
     4141 tggcacgctt cctggaggac ttcaacggcg tcctaccgcg cccgtggcgg ctcgacagcg
     4201 actccgcctc attcagccgc ggagccagcg ccgtcggctt cggcgtcgcc cgcgtcgcca
     4261 cgccgagcgg cggcggcagc ggtggccgcc atccgctctc cggccgcctc tgcccgggga
     4321 ccttccgcgt gctcaacaac gaccgcatcg gctccttgcg gctgccgcgc ggcaactaca
     4381 cgatcactgc gctgtcgctc aaccggccga cgtgcagccg tgcctcgcgc ctgttcacgc
     4441 gcttcctgaa cgacttcacc ggcgatcttc cggggcgctg gtcgctcgat ccccagaccg
     4501 cgaccttccg tcgcggcgcc gctagcttcg gcttccgcgt gaagccggta agtgactagt
     4561 ttcgaggcct acgagcgggc gtgcgcgggg ctcgacgccc cgttcgcgct cgtcgatctc
     4621 gatgccatgt ggcacaacgc ggcgtcgatg ctcgcccgcg cggcaggcaa gccgatccgc
     4681 gtggcgagca agtcggtccg ctgccgcgag ctgctgaacc gcatccttgg gcgagacccc
     4741 gggttcaggg gcctgatgac gttcacgctg gccgagaccc tctggctgcg cgggcagggt
     4801 ttcggcgacc tgctgctcgc gtatccaacc gccgaccgcg cctcgctcgc cgagctcgcg
     4861 cgagtcgagg gcgacgaccg gccgatcgtg atggtcgact ccgccgagca gctcgactgg
     4921 atcgcgcgag tggcgccgaa ccccgccgcg ccgctgcgcc tgtgcgtgga gttcgacacc
     4981 ggctaccggc tgccgcgcgg gctggcgacg atcgggccga agcgctcgcc gatccgcacg
     5041 ccggcgcagg cggcggcgct cgcgcgcgag atcgatcgcc gtccacagct cgagctcgcc
     5101 gggctgatgg gctatgaggg ccacatcgcc ggggtcggcg accggccgct gcgcaagccg
     5161 ctgcagggcg cggcgatccg cgccatgcag cgcgcgtcga tcgccgagat cgccgagcgg
     5221 cgcgcggcga tcgtcgccgc cgtgcgcgat gtcgtgccgg tcgagctggt caacggcggc
     5281 ggcacgggca gcctgcagac gacggctgcc gagggcgccg tcaccgaggt caccgcgggc
     5341 tctggcttct acgcctccac cctcttcgat cgctactcgg ctttcgacca gcgcccggcg
     5401 gcgatgttcg cgctgccggt cgtgcgccgt ccgcgcgccg acaccgtgac gctgctcggc
     5461 ggcggctatg tggcctccgg cccggccggc aaggaccgcc tgcccgagcc gttcctgcca
     5521 gcgggcctct cactcgacgc gctcgagggc gccggcgagg tccaaacccc ggtcaggggc
     5581 gccgcggccc gctcgctgcg gatcggcgac aacgtctacc tgcgccatgc gaaggccggc
     5641 gagctctgcg agcgcttcga cacgctccac ctgctcgagg gcgggcagat cgtcgacgag
     5701 gtgcccacct accgcggtga gggccatgcg ttcctctagc cggctcgccc tgctcgcgct
     5761 gacgctcgtc gcgctgctgg cgccggcagc cgccgacgcg aagcccaaca tcgtcttcat
     5821 catgaccgac gaccagaccg acgagtcgac ggcggtgcag cgcaacgtcg gcctgatcaa
     5881 gggcgacggc accaccttca ccgagacgat cgccagctat ccgctctgct gtccgtcgcg
     5941 cgccacctac ctctccgggc agtactcgca caaccacggc gtgatccaca acgccgggcc
     6001 gttcggcggc tacatccgct tcgacaactc gaacgcgctg ccgctctggc tccagcaggc
     6061 cggctaccgg acgatgcacg tcggtcgcta cctgaacggc tacggcgtcc agaacccgaa
     6121 catcgccgag gtgccgcccg gctggaacga ctgggtctcg ggcgtcggcg agcaggtgtt
     6181 caactacgag cgctggcaga ccaacgagaa cggccagatc ttcaaccgcc ccggccccga
     6241 ccacccgaac gagcatcaga ccgacttcta cgggcggcgc gccgccgagc taatcgacca
     6301 agccgcccca tcggcgcagc cgttctacct ctcgctgacc ttcccggcgc cgcacagcgg
     6361 ctttccgctg gagccggacg acccgccgtt cctgccgacg ccgtacccgg cgccgcgtca
     6421 ccgcaacgcc ttcgccggca cgccgctgcc gcggccgccc aacttcgacg aggccgacgt
     6481 cagtcgcaag ccgcagatcg tctcggaccg cccccgcctg tcgccggagt gggtggccgc
     6541 gatccaggag aactaccagc aggagctgga gtcgctgctt tcggtcgacg atgccgtcgg
     6601 tgcggtcctc aacgcgctcc agcgcagcgg cgagctcgac gacacgctgc tgatctacac
     6661 gtcggacaac gggttcttcc atggcgagca ccggctgccg tccgagaaga tcctgcccta
     6721 cgacgaggcg gccaggatcc ccttgatcat gcgggggccc ggcgtcccgc gcggagtcga
     6781 gctcgacaac ctggtctcca acatcgactg ggcgccgaca atcgtcgacg ccgccgacgc
     6841 ccgtgccggc cggctgatgg acggcacgtc gctgttcgag acgatcgacg acccgcgtcg
     6901 cttgccagga cgcgagatcg tgctcgagaa cgggaccggc gccaacggca taccgatgtt
     6961 ccgcgcgctg cgcaacgagc gctacctctg gatcgagcac aagttcagtg gcgagtacga
     7021 gctctacgac ttggtcaagg acccgtacca gctgcgcaat ctcgaggacc tcgactccta
     7081 cgcggagatc cgcgccgcgc tggccaagcg tctgcgccgg ctgcagcgct gtcgcggcgc
     7141 gcgcgcctgc ggggcgagcc gtccgaagct gaggctcacc acgcggcagg agcagccgcc
     7201 cgagccgaag cgcgagcggc cgcgcggcga ggcacaggcg acggccagct gcgtcggcag
     7261 agacttgcgc ctcggcatcg ccggccgcga gctcggagaa gtggtccgcg tcgactacta
     7321 ccgcggcaag cgcaagctgc gcgccacgcg acggacgccg ttcgaggtcc gcgtccggcg
     7381 gcgtgcactg ccgcgcggcc gcaagctgac gctgcgcgcg ctcgtgacga cggtggacgg
     7441 tcgcaaggcg acctacgacc gtcgcgtgca aacctgccgc cggtgagcga cggcttcgac
     7501 tacgactggc tagtcatcgg ctccggcttc ggcggcagcg tgtcggcgct gcgactggcc
     7561 gagaagggct actcggtcgg ggtgctggag tgcggccgcc gcttcgagga cggcgatctg
     7621 ccgagctcga cgtgggacgt gcgccgctac tcctggcgcc cccgcctcgg gctgcgcggg
     7681 atattccgga tcacgaactt caaggacgtg accgtcatat ccggctgcgg cgtcggtggc
     7741 ggcagcctcg gctacgcgaa cacgctctac gtgccgccgc gacgcttctt cgacgatccg
     7801 cagtggagcg acctcgccga ctggcacgcc gagctgtcgc cccactacgc cgaggccgag
     7861 cggatgctcg gcgtcgtcga gtacgaccag gacgacccgg ccggcgacct cctgcgcgag
     7921 ttcggcgagg agctcggggt cgctgacacc tacggccgcg cgcgcgtcgg gatcttcctc
     7981 ggcgagccgg gcaagacggt gcccgacccc tacttcggcg gcgaggggcc cgaccgcacc
     8041 ggttgcaccc gctgcggccg ctgcatggtc ggctgtccgc acggcgccaa gaactcgctg
     8101 atcaagaact acctgtggct ggccgagcgc cgcggcgcga aggtcgtcgc cgagcgcgag
     8161 gtcgtcgacg tgctcccgct cggagccgcc gacggctccg agggctatgc cgtgatcagc
     8221 gagcgccccg gcgcctggcg gcgccgcgac cggcgcctgc acaccgcccg tggcgtggtc
     8281 ctcgccgccg gaccgctcgg cacgaacaag ctgctggcca actgccgtca tcgcgggtcg
     8341 ctgccgcgcc tgtcgcgccg cctcggcgat ctggtccgca ccaacagcga ggcgatcctc
     8401 gcggtgaccg tcccggacga cagcgtcgac tacaccaagc gggtagcgat cacgtcgagc
     8461 atctaccccg accccgacac ccacgtcgag acggtcgtct acggccacgc gggcggcgtg
     8521 acgaagctct tcaacacgct gctggtcggc gacggaacgc gagtgacgcg accgctcaag
     8581 ctgctcggca cgctgctgcg ccatcccggc cggatgctca agctgctcgc ggctccggga
     8641 tggtcgcgcc gcacgatcat cctgctcgtc atgcagacgc tcgacaacgc gatcgcgctg
     8701 cggccgcggc caactcgcga cgggggcgtc cggctgcaaa ccgagcagga tccgcagcgg
     8761 ccgatcccga cgttcattcc cgtcgccaac caggccgccg agtggttcgc cgagcgcacg
     8821 ggcgggatcg cccagagctc gatccccgag gccgccctca acatcccgat gaccgcgcac
     8881 atcctcggcg gcgcagtgat cggcgccgat cccgagagcg gcgtggtcga ccgcgaccag
     8941 cgtgtcttcg gctacgagaa cctgctcgtc tgcgacggcg ccgccgtgcc agccaatccc
     9001 ggcgtcaatc ccagcctcac gatcacggcg ctggccgagc acgcgatgag ccgcatcccc
     9061 gccaaggatg ggccgctcgc ccggaccgcg gctgagcccg cggcgagcgc gtagacgggc
     9121 ggcgctgagg ccctagccgg ccgccgcccg ctccgcggca gcctcgatcc ggtctgggtc
     9181 cggctcccag tgggcctccg ggtcgatcgg cgtgtcgcgc aggtaggact cggcgtcgag
     9241 cgcggcggcg cagccggagc cggccgccgt gatggcctgg cggtaggtgt ggtcgatcag
     9301 gtcgccggcg gcgaagacgc cgccgaggcc ggtgcggctc gagcggccct cgaccttgac
     9361 gtagccgttc tcgtcgacct cgacctggtc ggcgacgatc tgcgagttcg gcgagtggcc
     9421 gatcgcgatg aacgcgccgt cgatctccag cacgcggtcc tcgccgccct cgctgtgcga
     9481 cagcaccgcg cgcgccagcg acccgctctc gccggcctcg aagcgctcga cgacgaacgg
     9541 cgtcaccagc tcgatgttct cggtctcgcg cgcgcggtcg agcatgatcg ccgaggcgcg
     9601 gaactcctca cgccggtgaa cgaccttgac gtcggctgcg aacttcgcga ggaaggtcgc
     9661 ctcctccatc gcggtgtcgc cgccaccgac gatgatcgtg cgcttgccgc ggtagaaggc
     9721 ggcgtcgcag gtcgcgcagt acgagacgcc gcgggcggcg agctcctcct cgccgggcac
     9781 cccgagcttg cgcggatcgg cgcccatcgc gagcacgacg ctgcggccgc ggtactcctg
     9841 atcgccgacc cagacccgct ggaggccgcc gtcggacagc tcgaggcgcg tcgcgtcatc
     9901 ggtcacgaag cgggtgccga agcgctcggc ctgagcgcgg aactgctgca tcatctccgg
     9961 ccccatcacg ccctcgggat agcccggata gttctcgacg tcggtcgtct gctgcagcag
    10021 gccaccccac tggaacccct cgatgacgag cgggtcgagg tcggcgcgcg ccgtatagag
    10081 cgcggccgta taaccggcag ggccactgcc gacgatgatc acgtcctcga tctgcttcgc
    10141 gccgtccgtc gcggtcattg agcctgtgcc tccgctttca ctttactttt tgaagtgtag
    10201 tccgcccgtc cagcatccca gccggcgacg gtgcgccgca gctggaagaa cgcgacgatg
    10261 ccgggcggga tcggcagcca gaacgcgatc acccggtatg tgagcacagc ggggaagacg
    10321 atgcccgcag ggatcccaaa gagcacgaag gcgccgatca tcccggcatc gacggatccg
    10381 acgccgcccg ccggcgacgg gatcaggttg gccgccatgc cgacgaagaa gccctgtacg
    10441 aggacgccga acgggacgct gccgccaaac gcctcgaagc tcgcccacag cacgccgatg
    10501 ttcgcggccc agaagccgat cgccccgcag accgcgagcg ctccgcggcg cgggtggcgg
    10561 acgtaggcga ccgcggtccg taggccggtc gccgcggtcg ccggcaccgg cgccagcttc
    10621 gcggcgacgc gcgcgaccct gcccctcccc ctgaagctcg acagccgtcg ctcgagatcg
    10681 cccgggatca gcgcgatcag acccgcgacg agcatcgcgc cgccggccag ccccgccggc
    10741 acgatcgttc ccgcgacggg cgcgtcgccc ggcagtaccc ccgtgcgcag cagcacgccg
    10801 aaaaccacta gcgcgatgcc gtagaccgag tacatcagca ccaggaaggc gaccatccgg
    10861 caggccgagc gccgccgcgg catcccggcc ttgcgcagcg cccagtaggt cagcacgatc
    10921 ccgccggcgc cggcggccga gaagatccgc gtcgccgcca ggccggccat cgtgatctgg
    10981 tagctcgccc cggcgtcgag ccggcggcga acgccagccg aggcattgcc gccgagcacg
    11041 ccgcggaaga gcgcgacgta ggcaccgaag gcggcgacgt tgaaggcgat cgcgatcgcg
    11101 acccagtacc aggcggcgtg gctgatctgc gcgatctcgc cgttgagtcc gacgacgcgc
    11161 ggaaacagga cgtagatcgc cgcgatcagc gcgagcacga acgcgacgcc gaacgccagc
    11221 cggcgacgat cgagcagcac cgcccccatt cccgatacgc gcaggtcctc gtcgacgtcc
    11281 tcctcgaaga cctccacgtc gtgcatcagg tcgctccggg ctccagtcgc tggatcttcc
    11341 gtgccccgcg caggaccgcc ggcgccgcaa gcggcgttcc gcggcggacg gcctcggcga
    11401 gcgcgtagcc ggcgagcagg tcgacgacgt agtgctcgcc gaggtagacc agtccgaagc
    11461 cgagcgtcag cgcatatccc caggccagcg cccccggccc acggccgacg tcggacagca
    11521 ctcgggcggc catgacggat gtgccgaagt gcagtgatgg catggcagcg aaggggttgc
    11581 cctccagccc atcgtagagc ggtcgccaga agcgcttcca gaagtgctcg ccggcctcga
    11641 ccatgatccg ccgcaccggc gccatgcgac cgctctcggc ggcgtaccag ggcggggccg
    11701 tcgggatcgc ccagtagacg acgaggccga ggtcgaacgt tgccgcggtc atcgccgccc
    11761 cgcgcacgaa ccggtcgtgg tggcgcagca gcaggaacgc gacggtgccg tgcggcacga
    11821 tgaaccaggc ccagtggacc atcgccagcg cgaggtcgtg gcgtcgcacc tcaccgggcg
    11881 tcccgaatgc ccgctggagc cgggtcgtcg gcgtctcgcc gcagccgatc aggcggtcga
    11941 tgcggatcgg atagtcgacc cgcgtgcgcc gcagcagcgc gtcgggatcg tcgtcgggca
    12001 tgtcgtagtg ggcgtagtag gcccacatct gcagcgcgta gatgcccgcg tcgcgcaggc
    12061 gcgtgcgcgg ggtcgccagc gccagggcga tcggcgcctg gaacgacagc gccgtgacga
    12121 ccggcggcct caggcgcagg cggtgacgca gcagcggtgt ggcgaccccg accgcgacgg
    12181 cgccccacgc gagcgttcgg agggcgcgcg agcttcgcaa accggcggca gcatagctct
    12241 caggcaggag gccggaacag gcgtacgtct acgggggcgc gctgggcgaa cgcctcggcg
    12301 tcggcgcggc tgccgaggtg actgccccag tgagacggca ccgcgacccg tggctggatc
    12361 cgccgcgccg cctcgacggc ctcgtcgacc gtcatcacgt aggtgccgca gaccggcagc
    12421 agcgcgacgt cgacgccggt cacggcgtcc atctccggga tcacgtcggt gtcgccggcg
    12481 tggtagagcc gctcgccgcg cacgttgagg tcgaagccga cccagccggc ggtgcgcgga
    12541 tggaagggat tgccgtcgct gtcgcgcttc gacgtgttgt acgcggctac cgcggtgacg
    12601 tcgatcccgc gcaggtggtc gagctcgatc gtctcccccg gcgcgatcga ggccacccgg
    12661 ccgcgcgccc gctccgccac cgcggcgggc gcgatcagcc aggtgcggtc gtgcgacagc
    12721 gcctcgacgt cgcgcggcga gtagtggtcg tagtgcccgt gggtgatcag gaccgcgtcg
    12781 ccgggcggag cgccgggcgg gacgcgatag gggtcgatgt agacggtcgc gcccttgacc
    12841 tcgaccctga atccggcatg gccgagccag accacggagt cgatcagcat tcggccagga
    12901 tacggttgcc gccgcggacc gcccgcggca atcgaacaca ggagagcgcg ttgagctacg
    12961 agacggtcaa gtgggagcac gagggcggcg tcggccggat cgtcctcgac cggcccgaga
    13021 ccctcaacgc ctggaacacg cagttcggcc acgacctcaa ggccgtcgtc gcggaggccg
    13081 ccgacgacga gtcggtgcgc gcggtcctgc tgaccggcgc cggtcgcgcc ttctcctccg
    13141 gggccgacct caaggccggc ttcgaggagg ccgccgacga cgggatgccc gacatccgcg
    13201 gccgcctcca cgacctcttc cacccgagcc tggttggcct gcgcacgatg ccgaagccgg
    13261 tcgtagtagc ggtcaacgga ccggccgccg gtatcggcgt gtcgctcgcg ctcgccggcg
    13321 acctcgtgct ggccgccgag tcggccttct tccagctcgc gttcgcgaac atcggcctga
    13381 tgcccgacgg cggctcgacc ctgttcgtcc ccgcgcgggt cggcaaggcg cgtgccttcg
    13441 agatggcgct gctcgccgag cggatcccgg cggcgcaggc gctcgactgg gggctggtca
    13501 acgccgtcca ccccgacggc gacctgatcg atgccgcgag cgccctggcc gagcggctcg
    13561 ccagcggtcc gacgcgcgcc tacgcgagcg ccaagcaggc gctcaacgcg acgatctacc
    13621 cgaacctcga cgagcagctc gagctcgagg ccgagctcca gcacgcgctg ggtcgctcaa
    13681 aggacttcat ggagggcggc gtcgccttcg tcgagaagcg cacgcccgag ttcacgggcc
    13741 gctagcggcg accgcgggtc cggaggcccg agcccccggt tattattccg cgccccgtgc
    13801 cggtcgttcg tcctccccac ctccgtcgcg ccctgctcgc cctcgcggca ggcgtgctgc
    13861 tgtcgctgct gatcgccccc ccggcgacag ccgacctgtt cacgccggag tcgggcggtt
    13921 cgccgaacgc cgacagcatc gactcgctgt acaaggtcat cttctacgtc ggcgtcgtga
    13981 tcttcctgat cgtcgagggg acgctgatct gggcgctggt gaagtaccgc gcccgtcgcg
    14041 gcggcccgca ggagggcgcg cagatccacg gcaacacgcc gctggagctc ggctggacgc
    14101 tcggcgcggc gacgatcgtg ctggtactcg ccgtcgtgac gttcctctac ctcggcgaca
    14161 tcaagaaccc gcccgcttcc gggcccgacg gcctggcgcg cggcgtggag gtcgcgtcga
    14221 tcgaccagcc cgagccgccg aaggggtcga gcagggcgct gacgatcgac gtcaacggcc
    14281 agcagttcct ctggcgctac gactacccgg gcggcgcgtt cagctactac gagatggtcg
    14341 tgccgaccga cacgacggtc gtgctcaacc tcacctcctc cgacgtcatc cactcctggt
    14401 ggatcccgaa gctcggcggc aagatggaca ccgtcccggg ctacgtcaac aaggcgtggt
    14461 tcaagatctc cgagccgggc gtctacatcg gccagtgcgc cgagctctgc ggctccggcc
    14521 acgccgacat gcgcgcccgc gtccgcgccg tctcccctga gcagtacacg gccttcctcg
    14581 agcgccagaa ggccgatctg aaggcgtccc aggaggcgct cgccgagcag cggcgacagc
    14641 gcgtcgaggc cgagaacgag atccagtagg aaggaccgag cgaccttggc cacccccgat
    14701 cccgccaccc ccacccccgc aggcaccgcg gcgctgcgcc ccgaggtcgt catgcacggc
    14761 ctctcgcccg agccgaaggg ctggctcgcc tggctgacga cgaccgatca caagcggatc
    14821 gggatcatgt acttcgtcgc caccttcatc ttcttcatcc tcggtggcgt cgaggcgctg
    14881 atcatgcgcg cccagctgat ccagccggac agcacgctga tcgcgcccga gacctacaac
    14941 ggcctcgtgt cgatgcacgg cacgacgatg atcttcctct tcatcgtccc ggtgatggcg
    15001 gccttcggca actacttcgt gccgctgatg atcggcgcgc gcgacatggc gttcccgcgc
    15061 ctcaacgcgc tgtcgttctg gctgctggcg ttcggcggga tcgccttcta cggcagcctc
    15121 ttcttcgagc cgccgcaggc gggctggacg tcgtacacgc cgctctccga cgacgcgtac
    15181 atgcccggcg gcgggatcga cgcgtggatc ttcctcgtcc acctcaccgg cctgtcgtcg
    15241 ctggtcggcg cgatcaactt cgtcgcgacg atccacaaca tgagggcacc cggcatgagc
    15301 tggggccgga tgccgctgtt cgtctggtcg atcctcgtct acagctacct gctgatcgcc
    15361 gcactgccgg cgatcgccgc ggccgtgacg atgctgctcg cggatcgcca cttcggcacc
    15421 gccttcttcg acccgaccgg cggcggcgac ccgatgctct ggcagcacct gttctggttc
    15481 ttcggccacc ccgaggtcta catcatgatc ctgccggcgt tcgggatcat ctcggaggtc
    15541 ctgccggtct tcgcgcgcaa gccgatcttc ggctacaagg ccatcgcggc gtcgacggtg
    15601 gcgatcgcgt tcctgggcct gctggtctgg gcgcaccaca tgttcacgac gccgacgtcg
    15661 acggtcgtgc tgatcttctt catgctgtcg tcgttcgcga tcgcagtgcc cacgggcata
    15721 aagatcttca attggatcgc gactctgtgg aagggctcga tcgtcttcaa gacgccgttg
    15781 ctgttcgcgg ccgcgctgcc cgcgctgttc gtgatcggcg gcatctccgg cgtgatcctg
    15841 gccgtcttcc cggtcgactg gcagctgcac gacacctact tcgtcgtggc ccacctgcac
    15901 tacgtgctgt tcggcggctc ggtcttcgga atcttcgccg ggatctacta ctggttcccg
    15961 aagatgaccg gccggatgat gggcgagggc ctcggcaagc tctccttctg ggtcatgttc
    16021 gtcggcttca acatgacctt cctcgtccag cactcggccg gcctctcggg catgccgcgg
    16081 cgcgtctacg actactccgg cgacctcggc gtcaccgact acaacctgat ctcgacgatc
    16141 ggctcgttcc tgctcggcat cggcgtactg ctcagcgtga tcaacgccct gcgctcctac
    16201 cgccacggca aggtcgcggg caacgatccg tggaagggca acacgctcga gtggttcgtc
    16261 cagtcgccgc cgccggtcaa caacttcgac gtcgttccgc gcgtccgctc ggtcgagccg
    16321 atgaaggaca tccgccgcga ggtcgc
//
LOCUS       DATABV010000217        22003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_553072, whole genome shotgun sequence.
ACCESSION   DATABV010000217 DATABV010000000
VERSION     DATABV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22003
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553072"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="VHF88_10015"
     CDS             complement(<1..1639)
                     /locus_tag="VHF88_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162140.1"
                     /translation="MPRLSRLVPALVAALALTCLAAAAPATAGSYTVRQCDYAAGNGH
                     HDFQWQSAGVPTVDPHAGSGCGEFGLAVRNGGGGTQRTYPSGAYGGWFAYAPNGTVIT
                     HFSGAFGVLEGSCCVSGMASYAEATQTHDGQGARAYLFQGHLGNDSWYAPSGLQGPVG
                     RSWSSSTSGFDAKRVGFHLRCGPGFSCSQGIYADLRLRGRSFDFTLRDDVAPAVGDPA
                     GTLVSGGWVRGVRTISFPAHDTGAGLAAIEVGADNGMALASPSICVVVAGRYARLQPC
                     PSARTGYWSVDTAQLPDGARQLHLRATDASGTTTTRTVAAYVDNGAPGTPRDVVVAGG
                     EGWRAANGFELAWTNPGGQHAPIALAHWRACRVGSASTECVAGTRGATGVAAAGPIAL
                     PGAGEWDVRVWLEDAVGNGDPESASEARRLRFDPDPPELQILPEEPGAPGRFVVATSD
                     RSGIADGAIELRRQGADGWTELATVRTEGRMTAELDPSRPPQAGFYDVRVRAVDAAGN
                     RAEADDVRAFGEPIPPQFDMGRLDHLLLGQAAPPRPTQ"
     gene            complement(1710..3098)
                     /gene="lpdA"
                     /locus_tag="VHF88_10020"
     CDS             complement(1710..3098)
                     /gene="lpdA"
                     /locus_tag="VHF88_10020"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX2162141.1"
                     /translation="MASSPSFDCVVIGSGPGGYVAAIRAAQLGMKTAVVERADTVGGR
                     CLNEACIPAKAVLRVADAYKEVTGADRFGITTGDVGFDFGGAAGHRDKVIKTMVGGVS
                     MLFEKNGIEVIRGQGSLTEDGNVKVGGSFDGQEIEAGKVVLATGSIAKPLLGLQFGGR
                     ILDTAAMWLTSEQPRRLAVIGAGASGTEVASAFGRLGTEVTLIEALDQILPLEDKDAA
                     RACAREIAKQNVKIVTGANVESAQDTGDAVTMVLNGEQQEFDLLCIAAGRAADAEGLA
                     LDEAGVKTDERGLIEVDGALRTSKEGVYAIGDLVPGPALAHKASDEGIIAVEDAAGLP
                     THAIDYPYVPRVTFCSPQVASFGLTEAQAKEAGHDVVVGKIPMGAVGAPTVYGDRTGL
                     VKIVGDKRYGEILGAHVVSPKAAELIQELVVARDLEGGYPELARSIHPHPAFAETILE
                     AARATDGWLIHG"
     gene            complement(3125..3691)
                     /locus_tag="VHF88_10025"
     CDS             complement(3125..3691)
                     /locus_tag="VHF88_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162142.1"
                     /translation="MAQRSSRKRRKQRRRAAGERGDAASVASGNGQQPPPPDDAMARG
                     YARGRAKDDAARAALVPLAEGERPGVVTVAAVVAFALGAANLVSYLAGVEVQGERPAF
                     TGILVYSGLMFTVAYGCFRARYWGVLGMQAMLALALLIFGVLLTRAESVVALLIAVAV
                     IGLAGTLFWKLVKAMARIQMPERPGSDR"
     gene            complement(3675..4331)
                     /gene="thiE"
                     /locus_tag="VHF88_10030"
     CDS             complement(3675..4331)
                     /gene="thiE"
                     /locus_tag="VHF88_10030"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX2162143.1"
                     /translation="MTTAAERRARLAQARVYLVADGGMEDDALAAALDGGVDMVQLRD
                     PALDDDALLARAAAWRALCDAHGALLWVNDRPDLALRAGADGVHVGQEDMSVAAAREA
                     GGSELLVGLSTHSREQLEAGIAAGADQLSVGPVWETPTKPGRPAAGLDYVRAVAALAP
                     PQPWFAIGGIDGGNVDEVAAAGAERVVVVRAIRDAADPGAAAAFLRAGLEEASLGAAQ
                     "
     gene            4383..5538
                     /gene="thiO"
                     /locus_tag="VHF88_10035"
                     /pseudo
     CDS             4383..5538
                     /gene="thiO"
                     /locus_tag="VHF88_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
     gene            5535..5747
                     /gene="thiS"
                     /locus_tag="VHF88_10040"
     CDS             5535..5747
                     /gene="thiS"
                     /locus_tag="VHF88_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX2162144.1"
                     /translation="MTLTVTLNGEPHELPDGATVADAVEAVGAPADGRGVAAAVGGEV
                     VPRGAWATHPLASDDRVEVLQAVQGG"
     gene            5752..6522
                     /locus_tag="VHF88_10045"
     CDS             5752..6522
                     /locus_tag="VHF88_10045"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX2162145.1"
                     /translation="MSALEIADRSFDSRLIVGTGGFRSLDSMRAAVRASGAEMATVAL
                     RRVDPAARGSILEVLDDCGCFVLPNTAGCFTARDAVTTARLAREALETDWVKLEVIGD
                     DKTLLPDPGELLDAAEELVADGFTVLPYTNDDPILARRLEDAGCAAVMPLGSPIGSGM
                     GIANPYNLRLIVEAARVPVILDAGIGTASDAALAMEAGCDAVLLASAVSRARDPEAMA
                     TAMRKAVEAGYAARHAGRIPRRLYGEASSPVEGVAELT"
     gene            6519..7007
                     /locus_tag="VHF88_10050"
     CDS             6519..7007
                     /locus_tag="VHF88_10050"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2162146.1"
                     /translation="MTGEQLPEQTALDRLAASWTAAWTTADGFAACCSAELTYEDPVA
                     AQPLSGLVELEAHAAKLREAFPDVRVEQTAPPLARGDYACVPWRLLGPNRGALAMLPA
                     TDRFVTLHGLHYLELTDGRVRRARGFLDLYDAATQLGLLPARGGWGEQALMLLRGFGF
                     RR"
     gene            complement(7012..8388)
                     /gene="rlmD"
                     /locus_tag="VHF88_10055"
     CDS             complement(7012..8388)
                     /gene="rlmD"
                     /locus_tag="VHF88_10055"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX2162147.1"
                     /translation="MAETPTERRSKPRRDDELEVTIESLAYGGAGVGRADGFVVFVRG
                     ALPGDRVRARIGKSKRSYAEATVAELLEPSPDRIEPAIPHPGAPWQVLPYERQLEVKQ
                     GQVVEALERLGGFEDPPVEPIVPAVEQLRYRNKVEYSFGQDEDGRMVLGSHRAGRWDV
                     IDELTVDVLASERVDELRNAVADWCRDNDVPAYDRRDQSGVLRNLVIREGRRTGELQA
                     RLVTRRRELEADALAAAVPADSFLVTNVEGLGETTLGGETELLKGQKKLFEELEIGGN
                     RLRFGISPEAFFQTNTEMAERLYAIAAELAGLSGRQRVFDLYCGIGTIGIALSLEARE
                     VVGVEIVEEAVADAIANAELNGIDNARFYAGDIRTAMRPLLEEEGTADVVVVDPPRAG
                     LSQKVVRRVLEVGAPTIVYVSCNPTTLAPNARQIVDAGYELVTVRPVDMFPQTPHIES
                     VALLRKPA"
     gene            complement(8397..8906)
                     /locus_tag="VHF88_10060"
     CDS             complement(8397..8906)
                     /locus_tag="VHF88_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162148.1"
                     /translation="MAEQRSSGTGGRKTDGTSPKAAGSGGVNELREALSSLIDPLGMV
                     MLTRERIEEALNDAVSRGRVTADDAQDLVASLIERGRRQADDVLGELEDLVSHKADRA
                     RAKAGKARRAAGMGATFPIAGYDDLTAAQVQDRLDGLAAADLRKVRDHEKRHANRKTV
                     LRAIDQRLK"
     gene            complement(8955..9773)
                     /locus_tag="VHF88_10065"
     CDS             complement(8955..9773)
                     /locus_tag="VHF88_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2162149.1"
                     /translation="MRLLSWNLFHGRDCPPNPALLTWRSRLLRHAERDATHVQVNRTL
                     GAEFAARLAGWEWDVALLQEAPPRWLGDLERATGAAGVRDLTSRNSLPALRARLADWN
                     PDLMRSAEGGSNMLLVRPPARIVATAAATLATTPERRRMLMARLELPAGRRLVVACLH
                     LSVPSTGQSADEALRAAEIATAWASGDSLVLGGDFNLRPVHHRAAFDGLRERFGLRRP
                     TGPRAIDHLLAGETVDAVAPPAALDAAEREVPGPGGLAIRLSDHAPVAASFGLR"
     gene            9786..10697
                     /gene="argF"
                     /locus_tag="VHF88_10070"
     CDS             9786..10697
                     /gene="argF"
                     /locus_tag="VHF88_10070"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX2162150.1"
                     /translation="MTRHFLTGAELTRAELTALIGRAAELKADRLASSALAGKSVALV
                     FEAPSTRTRVSFEVGVAELGGTPLLLRGDELQVSRGESPRDTALVLSRFVHAIGVRTG
                     DHAPLEELAEHGSIPIVNMLTRDHHPCQALADLLTLQERFGRLDGLRLAYVGDGNNVA
                     ASLMVLGAKAGVDVVVATPPQLAPAPAAVELATEVAEGAGRATVVTDPREAAAGAHAV
                     YADVWVSMGDDPSDAAARRVALEPYRLDDALLAAARDDAVALHCLPAHPGEEITADVL
                     YGPRSAVWDQAENRLHAQKALLELLVS"
     gene            10745..11170
                     /locus_tag="VHF88_10075"
     CDS             10745..11170
                     /locus_tag="VHF88_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX2162151.1"
                     /translation="MKQLLALICAVLALGLVAAGCGDDDDSSGDGGGSAATTEQTATT
                     EKQASGGGDAAAKQVEIEVVDIDYEPKDETVPSGTTVKWTNTGDLPHTVTKQDGPGPD
                     FDSGTMEPGDTYEETFTTPGKIAYHCTIHAGQDGSLTVE"
     gene            11200..11532
                     /locus_tag="VHF88_10080"
     CDS             11200..11532
                     /locus_tag="VHF88_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2162152.1"
                     /translation="MAPGELIRTTRVRRGLSQERLARRAGTRQSAISRLERGDVSPTV
                     ETLELLLNAMGERLELDGAPLERRYDPLHRRANAARSPEERLRLAISWNRMAGRLAAA
                     GREARGEA"
     gene            11519..11995
                     /locus_tag="VHF88_10085"
     CDS             11519..11995
                     /locus_tag="VHF88_10085"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX2162153.1"
                     /translation="MARPEPLDAERILRALAEHRVDYVLIGGLAVQTHGHVRTTNDAD
                     LIPAPNPANLERLAAALRALDARVLNPGSEDVPIDARMLPRATIWQFATPDGGVDVMH
                     EVPDGDPFEELSKRALRVRLGDVEVPVVSLDDLIRMKLARGRSIDLEDVAALTDPA"
     gene            12062..12775
                     /locus_tag="VHF88_10090"
     CDS             12062..12775
                     /locus_tag="VHF88_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX2162154.1"
                     /translation="MSAYYPGPNPPDAVELRVLGALIEKRRTTPDAYPLSVNSLRLAC
                     NQATNRDPVVEYDERTVLDALERLGRRGWTRLASGASSRARKYRHLFDEALGLVDDEL
                     SMLAVLMLRGPQTPGELKQRTDRLHPFADLAAVEDTLERLIDRELVARLPRRPGQKEQ
                     RYVQLLDGGDDEAPERVAAPATPVTTPAPPGFDGAPAPVDVPPAGLSSELEERVDSLE
                     RQVAELSAELRALRAELGP"
     gene            complement(12772..13203)
                     /locus_tag="VHF88_10095"
     CDS             complement(12772..13203)
                     /locus_tag="VHF88_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162155.1"
                     /translation="MAKVLVVANQTAESEELLTALRERDGQGDCEFTVLIPATPHGLS
                     WAANMDAEVGKREAERHRDALIGRLRDAGVDVEDAIVGDPDPLGAIQDACNRGSYDEL
                     IVSTLPHHISKWLHLDLPRKAAHATGLPVTTVEARAAAKTS"
     gene            13371..15911
                     /locus_tag="VHF88_10100"
     CDS             13371..15911
                     /locus_tag="VHF88_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX2162156.1"
                     /translation="MTLVVAAGLLQSAAAASGATLSGTVSGQSGDGQRQPVSGALVQV
                     IDPQSGDVVAVGTTGFAGEYSIGVAPSTYRVRFDPGPGQLWGSTTVDDIDVSSDRVLD
                     VVLILTGLVRVSGVVRDAAGLPVAGVSVSAAGGSGRTAADGSYSINAIPGTGRSVAVS
                     AGGLALLGLPESWSLRSSPFELQSDAVRDFVLPSAVRVTVRVLGVEDEPLEGVEVRVP
                     ELELAGSFGGVTGHARSGGGKYLTDANGEIARTVFGGDPLSSSSDVRPPQGSGYGSTT
                     YAMPPFTTDTTVVVRPPRLVRVSGVVRDAGGAAVAGARVAVGDGIGVSASDGSYSVET
                     APAEAGRLTVGAGATALPGLPSAWSLRSSFLDLRTDVVRDVTLPSTAHVTVRVLGVDD
                     EPLEGVQVRIPSFSLDGQFGDVTGTLVSGADGKRWTDADGEVSTATFGGVQVGSSQEV
                     RPPVGSGYGTTSYAMPPVTGDTTFVVRPPRQVRVTGFVRDAAGSPVAGASVSVTDGSG
                     TTAADGSYAIVATPGAARRLTVSGGPSMPGSVVSSPFALESDTVVDVDFPPLRLVTVR
                     TLGDGDEPLAGVEVRIPTFELSGTFGQVTGLARSGGGKYLTDADGEVSRLVFGGEPQS
                     SSNDARPPLGSGYGEATYAMPTVTGDTTVVLRFQSANADLVPPAIDCAAPPGDWQADN
                     VSLRCTASDLSAGLSDPSDAHFDLATHVPEGSEDADAETDTREVCDRAGNCAVAGPIG
                     GIRVDRQAPRIRFVQEPNGLQGWFVDAPGSTTAVADDSTIASLTCTRDGMPVLETPVA
                     GFGSLSLVMSAATEGRHAVECRRPTRSGTRPSRATRSSSTSPRLAGRP"
     gene            15965..16384
                     /locus_tag="VHF88_10105"
     CDS             15965..16384
                     /locus_tag="VHF88_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162157.1"
                     /translation="MTVSFVPDGDPLLADGSPGSGVDASTSTQPLTIDASGVHDVVGS
                     VADVAGNRSADRSTTVRVDADPPTSELTCPQDPPLGSTARARWEDDDGESGLDRRRRG
                     QMVVDTATVGFHTATHVASDNVGHQTSSTCVYRVIGN"
     gene            complement(16400..17005)
                     /locus_tag="VHF88_10110"
     CDS             complement(16400..17005)
                     /locus_tag="VHF88_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX2162158.1"
                     /translation="MPATDDEIREKYLERAIRELNQLTHEIQGCDHCPRGSLMPVLGS
                     GHPQADVFMLKFAPRPSEIEEGVAFYGRSGSALMKSFKRLGIDPLAVYGTVVVKCPVH
                     DTDLAAPECKARVLDELAIVQPRIVVAMGEEALEELNGLQVPLAAEVEATPGKIQKLT
                     ASCDALYVPDIDASLDDERSKRAFWAAFRSLGEWYADFPPY"
     gene            complement(17054..18958)
                     /locus_tag="VHF88_10115"
     CDS             complement(17054..18958)
                     /locus_tag="VHF88_10115"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX2162159.1"
                     /translation="MSDERFAIAQVTPYPWEQGHDVNRYVERVASELRERGHRVVVVA
                     PSDSRSLIRDGRARVKRVVRGDQGALFDGDVLAVGQSLPFRRGRAVSLPIDVSRTLED
                     LLEHAELDFVHVHEPFAPSAAGAALRHSRTLNVGTFHGTSERFLSTQVARRVVQLLFG
                     RLDARTATSATARDVVERFFPGHYGVVASGAELIERAGEGGPVEILYSADEDRGALRL
                     FVRALRKLRVDGDWRATIWLRDPAAVASPLQLPRRLRDRVRMAGPADGTEAEHLARAD
                     VVVAASTGVAPSSQLVMRALGAGAVPVVANLPQYEELLDDGELGLLFDPRDALALGAQ
                     LERLVSEPELREGFAARVAAAREGLSWSRAADEFEAIYATAAARRHDPDGKPAVRKRL
                     ADREFVHVDLHMHTDHSSDCATPAATLLEAAKAAGLDAIAITDHNEVSGAHEARVLAE
                     EMGGIQVIVAEEVKTADQGEVIGLFIEEKIPKGMTLQETIAAIRAQGGLVYVPHPFDR
                     MHSVPDYEHLLDVVEEIDAIEVFNPRVAFSAFNEEAARFAQKYRIVAGAGSDSHVAAG
                     LGSVKIRMRAFDGPEEFLESLRDADIVRKRQSLLYVQALKFIQTRATPHAARRRTAKP
                     GAGAKRAAKQ"
     gene            complement(18997..20166)
                     /locus_tag="VHF88_10120"
     CDS             complement(18997..20166)
                     /locus_tag="VHF88_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX2162160.1"
                     /translation="MSENVSTNGASRYQTEKVRVAIVGVGNCASAFVQGVEYYKDANP
                     DEDVPGLMHVDLGGYHVGDIEFTAAFDIDSDKVGKDLSEAIWSGQNNTIKFADVPHLD
                     VPVHRGMTHDGLGKYLKQKIEKAPGETADIVQILKDTKTDVVVSYLPVGSELATKWYV
                     EQVLEAGCGFVNCIPVFIAREDYWGKRFEKAGLPIVGDDIKSQVGATIVHRQLARLFG
                     DRGVKMMRTSQLNVGGNMDFYNMLERERLESKKISKTNAVTSIMDHELPKDDVYIGPS
                     DYVPWLTDRKWAHIRVEGQAFGDVPLNVELKLEVWDSPNSAGIVIDATRCCKLALNRG
                     IGGPLEAPSSYLMKSPPVQVPDSVARENVEAFIAENGGAPKLPGGTPVSSEPTEA"
     gene            complement(20320..20928)
                     /locus_tag="VHF88_10125"
     CDS             complement(20320..20928)
                     /locus_tag="VHF88_10125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2162161.1"
                     /translation="MAVRSAEMRALARNRLIESRLTPNAISMTGLVGNVIAAVLVTQR
                     YFFLAGIAFILGSVMDTLDGRYSRMSGKGTPFGAFLDSTLDRVEEGIVLTAIGAYFAS
                     RGDDLAVAAVVAAVLFSLMVSYTRARAEALGVECKVGLATRAVRVVILSVGLLFAKGA
                     GLGDFELLAPAIYVLAALTVITTFQRILHVRRALNGSAATLA"
     gene            complement(20928..21734)
                     /locus_tag="VHF88_10130"
     CDS             complement(20928..21734)
                     /locus_tag="VHF88_10130"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2162162.1"
                     /translation="MRQVYDDFTLQFRRAWRVTSRWLPQGWLDALRQLALFAGAYYVY
                     RIVRGLVDGRATLAFENARTLVDIERTLGLFFEPGLQAWALGEEWIVTGANWMYVNSH
                     FTITTTFLIWLYIFRNSSYYYVRNMFMIAMGLALVGYMAYPTAPPRYMPEWGFTDTVS
                     AFVGPQAEASANVLYNPFAAVPSMHVAFALMVGIPAMRIVQNRFAKVLWALYPAVVTF
                     VVVVTANHFWLDAALGALVAAISAWGASYALGRVRPDVWAWQDRRARAVA"
     gene            complement(21756..>22003)
                     /locus_tag="VHF88_10135"
     CDS             complement(21756..>22003)
                     /locus_tag="VHF88_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162163.1"
                     /translation="EGDAKIQLEQLGSDRVLLRVRRPGSALVRVRWTPYWLAKGGCVQ
                     RAGEWTRVTAEKPGFLPLVTRFSPERIFQRGPRCNSS"
BASE COUNT         3006 a         8194 c         7751 g         3052 t
ORIGIN      
        1 gctgcgtcgg ccgcggcggc gcagcctgtc cgagcagcag atggtcgagc cggcccatgt
       61 cgaactgtgg cgggatcggc tcgccgaacg cgcgcacgtc gtcggcctcg gcgcggttgc
      121 cggcggcgtc gacggcacgc acgcggacgt cgtagaagcc ggcctgcggc ggccgcgacg
      181 ggtcgagctc ggcggtcatc cggccctccg tgcggaccgt cgccagctcg gtccagccgt
      241 cggcgccctg gcggcgcagc tcgatcgcgc cgtctgcgat gcccgagcgg tcgctcgtcg
      301 cgacgacgaa ccgtccgggc gcgcccggct cctcggggag gatctggagc tccggcgggt
      361 cggggtcgaa gcgcagccgg cgcgcctccg acgcactctc ggggtcgccg ttgccaacag
      421 cgtcctcgag ccagacgcgg acgtcccact cgccggcgcc gggcagcgcg atcggtcccg
      481 ctgcggcgac gcccgtggcg ccgcgtgtgc cggcgacgca ctccgtcgat gccgacccga
      541 cacggcaggc gcgccagtgg gcgagcgcga tcggtgcgtg ctggccgccg gggttggtcc
      601 acgcgagctc gaagccgttg gcggcgcgcc acccctcgcc gcccgccacg accacatcgc
      661 gcggggtccc cggcgcgccg ttgtcgacgt aggcggcgac ggtccgggtg gtcgtcgtcc
      721 cgctcgcatc ggtggcccgt aggtggagct ggcgcgcgcc gtcgggtagc tgcgcggtgt
      781 cgaccgacca atagccagtg cgcgcgctcg ggcacggctg aagccgcgcg tagcggcccg
      841 cgacgaccac gcagatcgaa ggcgaggcga gcgccatgcc gttgtcggcg ccgacctcga
      901 tcgccgcgag gccggcaccg gtgtcgtgag ccgggaacga gatcgtccgc acgccgcgga
      961 cccagccgcc gctgaccagc gtccctgccg gatcgcctac cgctggcgcg acgtcgtcgc
     1021 gcagcgtgaa gtcgaacgag cgaccgcgca gtctcaggtc ggcgtagatg ccctgcgagc
     1081 acgagaaccc cggcccgcag cgcaggtgga agccgacccg cttggcgtcg aagccgctcg
     1141 tcgaagagct ccagctgcgg ccgaccgggc cctgcaggcc cgacggcgcg taccagctgt
     1201 cgttgccgag gtggccctgg aagaggtagg cgcgtgcgcc ctggccgtcg tgggtctggg
     1261 tcgcctcggc gtagctcgcc atcccgctga cgcagcagct gccctcgagc accccgaagg
     1321 ctcccgagaa gtgggtgatg acggtcccgt tcggcgcgta ggcgaaccag ccgccgtagg
     1381 cgcccgacgg gtaggtccgc tgcgtaccgc cgccgccgtt gcgcaccgcg aggccgaact
     1441 cgccgcagcc gctgccggcg tgcgggtcga ccgtcggcac gccggccgac tgccactgga
     1501 agtcgtggtg tccgttgccg gccgcgtagt cgcactgacg gaccgtgtag ctgccggcgg
     1561 tcgcgggcgc cgcggcggcg aggcaggtca gcgccagcgc ggcgacgagc gccgggacga
     1621 ggcgggaaag gcggggcacg gcgtctcctt cggtggggtg cttccaccaa gggacgcctg
     1681 ggggcctact cgcccccctc ggtcgctacc tatccgtgta tcagccagcc gtcggtcgcg
     1741 cgcgcggcct cgaggatcgt ctcggcgaac gcagggtgcg gatggatcga gcgtgcgagc
     1801 tcggggtagc caccctcgag gtcgcgggcg acgacgagct cctggatcag ctcggcggcc
     1861 ttcgggctga cgacgtgggc gccgaggatc tcgccgtaac gcttgtcgcc gacgatcttc
     1921 acgaggccgg tgcggtcgcc gtagacggtc ggagccccga cggcgcccat cgggatcttg
     1981 ccgacgacca cgtcgtggcc ggcctccttc gcctgcgcct cggtcagtcc gaagctcgcc
     2041 acctgcggcg agcagaaggt cacgcgcggc acgtaggggt agtcgatcgc gtgggtcggc
     2101 agcccggccg catcctcgac cgcgatgatc ccctcgtcgg acgccttgtg cgcgagcgcc
     2161 ggccccggca ccaggtcgcc gatcgcgtag acgccctcct tcgacgtgcg cagcgctccg
     2221 tcgacctcga tcagcccgcg ctcgtcggtc ttcacgccgg cctcgtcgag cgccaggccc
     2281 tcggcgtcgg ccgcgcggcc ggcggcgatg cagagcaggt cgaactcctg ctgctcgccg
     2341 ttgagcacca tcgtcaccgc gtcgccggtg tcctgcgccg actcgacgtt cgcgccggtc
     2401 acgatcttca cgttctgctt cgcgatctcg cgcgcgcagg cgcgggccgc gtccttgtcc
     2461 tcgagcggca ggatctggtc gagcgcctcg atcagcgtca cctcggtccc gagccgcccg
     2521 aacgccgagg cgacctcggt gcccgacgcg ccggcgccga tcacggcgag gcggcgcggc
     2581 tgctcggagg tcagccacat cgccgcggtg tcgaggatgc ggccgccgaa ctgcagccca
     2641 agcagcggct tggcgatcga tccggtcgcc aggacgacct tgccggcctc gatctcctgg
     2701 ccgtcgaacg aaccgccgac cttgacgttg ccgtcctcgg tcagcgaccc ctggccgcgg
     2761 atcacctcga tcccgttctt ctcgaacagc atcgagacgc cgccgaccat cgtcttgatc
     2821 accttgtcgc ggtggccggc cgcgccgccg aagtcgaagc cgacgtcgcc ggtcgtgatg
     2881 ccgaagcggt cggcgccggt gacctccttg taggcgtccg cgacgcgcag cacggccttc
     2941 gccgggatgc acgcctcgtt gaggcagcgg ccgccgacgg tgtcggcgcg ctcgacgacc
     3001 gcggtcttca tcccgagctg ggcggcgcgg atcgccgcga cgtagccgcc cggtccggag
     3061 ccgatcacga cgcagtcgaa ggagggggag ctcgccatgc ccgggccaga ctacccaagc
     3121 ggcctcagcg gtccgagccc gggcgctcgg gcatctggat ccgcgccatc gccttgacga
     3181 gcttccagaa gagcgtgccg gcgaggccga tcacggcgac cgcgatcaac agggcgacga
     3241 cgctctccgc gcgcgtcagc agcacgccga agatcagcag cgcgagcgcc agcatcgcct
     3301 gcatgccgag cacgccccaa tagcgggcgc ggaagcagcc gtaggcgacg gtgaacatca
     3361 gccccgagta gacgaggatg ccggtgaacg ccggccgctc gccctggacc tcgacgcccg
     3421 cgaggtagga gacaaggttt gccgcgccga gcgcgaaggc gaccaccgcc gcgaccgtca
     3481 cgacgccggg ccgctcgccc tcggcaagcg gcacgagtgc agcgcgcgcc gcgtcgtcct
     3541 tggcgcgtcc gcgggcgtag ccgcgcgcca tggcgtcgtc ggggggcggc ggctgctggc
     3601 cgttcccgga cgcgaccgag gcagcgtcgc cccgctcgcc ggcggcgcga cggcgctgct
     3661 tgcgccgttt gcggctactg cgctgcgcca aggctcgcct cctcgagtcc ggcgcgcagg
     3721 aaggccgccg cggcgcccgg gtcggccgcg tcgcggatcg cgcgcacgac gaccaccctt
     3781 tcggcgccgg ccgccgccac ctcgtcgacg ttgccgccgt cgatcccgcc gatcgcgaac
     3841 cacggctggg gcggcgccag cgccgccacc gcgcggacgt agtcgaggcc ggccgccggc
     3901 cgccccggct tggtcggcgt ctcccagacc gggccgacgc tgagctggtc ggccccggcg
     3961 gcgatgccgg cttcgagctg ctcccggctg tgcgtcgaca ggccgaccag cagctcgctg
     4021 ccgccggcct cgcgcgccgc cgcgacagac atgtcctcct gcccgacgtg gacgccgtcg
     4081 gcgcccgcgc gcagcgcgag gtcgggccgg tcgttgaccc acagcagcgc cccgtgggcg
     4141 tcgcagagcg cccgccacgc cgcggcgcgc gccagcaggg cgtcgtcgtc gagcgccggg
     4201 tcgcgcagct ggaccatgtc gacgccgccg tcgagcgcgg cggcgagtgc gtcgtcctcc
     4261 atcccgccgt cggcgacgag gtagacgcgc gcctgcgcca gccgcgcgcg gcgttccgct
     4321 gccgtggtca tctgtcccat cctggcagag gaagcccgtc gcgcaggggt accttgtatg
     4381 cgatggaacg cgcttacgac ctcgtcgtgg tcggcgccgg cgcgatcggc ctcagctgcg
     4441 catggcgcag cgcccaggcc ggcatgtcgg tgctcgtcgt cgagcgcgag gcgccgggcg
     4501 ccgccgcctc cggcatcgcg gccgggatgc tcgcgcccgt caccgaggcg acgttcggcg
     4561 agcaggcgct gctcgcgctc aacctcgacg cgcgcgccca gtggcccgcc ttcgccgccg
     4621 agctcgagga gctgaccgcg ctgccgaccg gctacgccga ctccggcgcg ctggtcgtcg
     4681 cggccgatcg cgacgacgcc gaggagctgc gtcgcctgca cgccttccag gtcgagctcg
     4741 gcctcgacgc ggagtggctc gcggcggatc ggtggcgcga tccacgcgcc gcaggacggc
     4801 caggccgatc cggccgcggt ggttcgagcc ctggccacgg cgttcagggc cgccggcggc
     4861 gagatccgga ccggttgcga ggtcatcggc atcgacaccg ccgcgggccg cgtcgccggc
     4921 gtggagatcg cgccggtcga cgtcgagcac gaccccgatc agctgcgcgt cgccgggctc
     4981 gacgccggag aggtagtgcc cgcgcacgcg gtgctcgtcg cggccggcgc ctggagcgcc
     5041 gagggcggcc tcgcgaccga cgcgggagcg ccgccggtgc gcccggtcaa gggccagctg
     5101 ctcgagctgc gcgtccgccg cgggcaccgg ctgccggcgc gccacatcgt ccgcacgccg
     5161 cgctgctacc tcgtcacccg gcccgacggc cgggtcgtgc tgggggccac ccaggaggag
     5221 cagggcttcg acaccgccgt gaccgccgag ggcgtccatc gcctgctcga ggcggcctgg
     5281 gaggtcctgc ccgacgccgg cgagctcgag ctggtcgccg cccgcgccgg cctgcggccc
     5341 ggcacgcccg acaacgcgcc cgccgtcggc gagggcgacg tcgaggggct gctctgggcc
     5401 accggccact accgcaacgg ggtgatgctc gcgccctcga ccgggcgcgc gatcgccgcg
     5461 ctgctcgtcg atggcgagct gccggagccg tgggcggcgt tcacgccgcg gcgcttcctc
     5521 gaggaggtgc gcgtgtgacg ctgaccgtga cgctcaacgg agagccgcac gagctgcccg
     5581 acggggcgac cgtcgccgac gccgtcgagg cagtcggcgc gcccgccgac ggacgcggtg
     5641 tcgcggccgc cgtcggcggc gaggtcgtgc cgcgcggcgc ctgggccacg catccgctgg
     5701 cctccgacga ccgcgtcgag gtgctgcagg cggttcaggg aggatgagcc ggtgagcgcc
     5761 ctcgagatcg ccgaccgctc gttcgactcg cggctgatcg tcggcaccgg cggcttccgc
     5821 agcctcgact cgatgcgcgc cgccgtgcgc gcctccggcg ccgagatggc gaccgtggcg
     5881 ctgcgccgcg tcgaccccgc cgcgcgcggc tcgatcctcg aggtgctcga cgactgcggc
     5941 tgcttcgtgc tgccgaacac cgccggctgc ttcaccgccc gcgacgccgt cacgaccgcc
     6001 cgcctcgcgc gcgaggcgct cgagaccgat tgggtcaagc tcgaggtgat cggcgacgac
     6061 aagacgctgc tacccgaccc cggcgagctg ctcgacgccg ccgaggagct cgtcgccgat
     6121 ggcttcaccg tgctgccgta caccaacgac gacccgatcc tcgcgcgccg gctcgaggac
     6181 gccggctgcg ccgccgtgat gccgctcggc tcgccgatcg gcagcggcat gggcatcgcc
     6241 aacccctaca acctgcgcct gatcgtcgag gccgcgcgcg tgccggtgat cctcgacgcc
     6301 gggatcggca ccgcgtccga cgccgcgctg gcgatggagg ccggctgcga cgccgtcctg
     6361 ctcgcgagcg ccgtctcgcg cgcgcgggac cccgaggcga tggcgaccgc gatgcgcaag
     6421 gccgtcgagg ccggctacgc cgcccgccac gccgggcgca tcccgcgccg cctctacggc
     6481 gaggcatcga gccccgtcga gggcgtcgcg gagctgacgt gaccggcgag cagcttccgg
     6541 agcagaccgc actcgaccgg ctcgccgcca gctggaccgc ggcctggacg accgccgacg
     6601 gcttcgccgc ctgctgcagc gccgagctga cctacgagga cccggtcgct gcgcagccgc
     6661 tcagcgggct cgtcgagctc gaggcgcacg ccgcgaagct gcgcgaggcg ttccccgacg
     6721 tgcgcgtcga gcagaccgcg ccgccgctcg cccgcggcga ctacgcctgc gtcccgtggc
     6781 ggctgctcgg ccccaaccgc ggcgcgctcg cgatgctgcc cgcgaccgac cgcttcgtca
     6841 ccctgcacgg cctccattac ctcgagctga ccgacggccg cgtccgccgc gcgcgcggct
     6901 tcctcgacct ctacgacgcc gccacccagc tcgggctgct gccggcgcgc ggcggctggg
     6961 gcgagcaggc gctgatgctc ttaagaggct tcggcttccg ccgctgagac gtcaggccgg
     7021 cttgcgaagc agtgcgaccg actcgatgtg cggcgtctgc ggaaacatgt cgaccggtcg
     7081 gacggtgacg agctcgtagc cggcgtcgac gatctgccgt gcgttcggcg cgagcgtcgt
     7141 cgggttgcac gagacgtaga cgatcgtcgg cgcgccgacc tcgagcacgc ggcggacgac
     7201 cttctgcgac aggccggcgc gcggcgggtc gacgacgacg acgtcggcgg tcccctcctc
     7261 ctcgagcagc ggtcgcatcg cggtgcggat gtcgccggcg tagaagcgcg cgttgtcgat
     7321 cccgttcagc tccgcgttgg cgatcgcgtc ggcgaccgcc tcctcgacga tctcgacgcc
     7381 gaccacctcg cgcgcctcga gcgacagcgc gatcccgatc gtcccgatgc cgcagtagag
     7441 gtcgaagacc cgctgacggc ccgacagccc cgccagctcg gcggcgatcg cgtagagccg
     7501 ctcggccatc tcggtgttgg tctggaagaa ggcctccggc gagatcccga agcgcaacct
     7561 gttcccgccg atctcgagct cctcgaacag cttcttctga cccttgagca gctcggtctc
     7621 gccgccgagc gtcgtctcgc cgagaccctc gacgttggtc acgaggaagc tgtcggcggg
     7681 gaccgcggcg gcgagcgcgt cggcctccag ctcccggcgg cgggtgacca gccgcgcctg
     7741 cagctcgccg gtgcgccggc cctcgcggat caccaggttg cgcagcacgc ccgactggtc
     7801 gcgcctgtcg taggcgggga cgtcgttgtc gcggcaccag tcggcgacgg cgttgcgcag
     7861 ctcgtcgacc cgctcggagg cgagcacgtc gacggtcagc tcgtcgatca cgtcccagcg
     7921 gccggcgcgg tgcgagccga gcaccatccg gccgtcctcg tcctggccga acgagtactc
     7981 gaccttgttg cggtagcgca gctgctcgac ggcggggacg atcggctcga ccggcgggtc
     8041 ctcgaagccg ccgaggcgct cgagcgcctc cacgacctgg ccctgcttga cctcgagctg
     8101 gcgctcgtag ggcagcacct gccacggtgc ccccgggtgc gggatcgccg gctcgatccg
     8161 atccggcgag ggctcgagca gctcggcgac cgtcgcctcg gcgtaggagc gcttcgactt
     8221 gccgatccgc gcgcgcaccc ggtcgccggg cagcgcgccg cgcacgaaga cgacgaagcc
     8281 gtcggcgcgc ccgacgcccg cgccgccgta ggcgagcgac tcgatcgtca cctccagctc
     8341 gtcgtcgcgc cgcggcttcg accgcctctc ggtaggggtc tcggccatgc cgtcctctac
     8401 ttcaaccgct ggtcgatcgc gcgcaggacg gtcttgcggt tggcgtggcg cttctcgtgg
     8461 tcgcgcacct tgcgcaggtc ggcggcggcg aggccgtcga gccggtcctg cacctgggcg
     8521 gcggtcaggt cgtcgtagcc ggcgatcggg aaggtcgcgc ccatgccggc ggcgcggcgg
     8581 gccttgccgg ccttcgcgcg cgcccggtcg gccttgtgcg agaccaggtc ctcgagctcg
     8641 ccgagcacgt cgtcggcctg gcggcgaccg cgctcgatca ggctcgcgac gaggtcctgc
     8701 gcgtcgtcgg cggtcacccg gccgcggcta acggcgtcgt tgagcgcctc ctcgatccgc
     8761 tcgcgggtca gcatcaccat gccgagcggg tcgatcaggc tgctgagcgc ctcgcgcagc
     8821 tcgttgacgc cgccggaccc ggccgccttc ggcgacgtgc cgtccgtctt gcgccccccg
     8881 gtgccgctgc tccgctgctc cgccatctgg aactgcctcc cggtcgagtc cgttcgctga
     8941 tctgcgacga gggactatcg caggccgaac gaggcggcga ccggtgcgtg gtcggacagt
     9001 ctgatcgcca gtccgccggg tccggggacc tcgcgctcgg cggcgtcgag cgcggcgggc
     9061 ggagcgaccg cgtcgaccgt ctcgcccgcg agcaggtggt cgatcgcgcg cggtccggtt
     9121 ggccgtctga gcccgaagcg ctcgcgcaga ccgtcgaacg cggcgcggtg gtggacgggg
     9181 cgcaggttga agtcgccgcc gaggacgagc gaatcgccgc tcgcccacgc ggtcgcgatc
     9241 tccgcggcgc gcagcgcctc gtccgcgctc tggccggtgc tcggcaccga caggtggagg
     9301 caggcgacca cgagtcgccg gcctgcgggc agctcgagcc gcgccatcag catccgccgc
     9361 cgctcgggcg tcgtcgccag cgtggccgcc gccgtggcga cgattcgcgc cggcgggcgg
     9421 accagcagca tgttcgagcc gccctccgcc gagcgcatca ggtcgggatt ccagtcggcc
     9481 aggcgcgcgc gcagcgctgg gagcgagttg cgcgacgtca ggtcgcgcac accggccgcg
     9541 ccggtcgccc gctcgaggtc gccgagccag cgcggcggcg cctcctggag cagcgcgacg
     9601 tcccactccc aaccggccag ccgggccgcg aactccgcgc ccagcgtcct gttcacctgc
     9661 acatgggtcg cgtcccgctc ggcgtggcgc agcagccgcg agcgccaggt caacagcgcc
     9721 gggttcggcg ggcagtcgcg cccgtggaac aggttccagc tgaggagccg caatacgatg
     9781 ccggggtgac tcgccatttc ctgaccggcg ccgagcttac gcgcgccgag ctgaccgcac
     9841 tgatcggccg cgccgccgag ctgaaggccg accggctcgc gtcgagcgcg ctcgccggca
     9901 agagcgtggc gctggtcttc gaggcgccgt cgacgcgcac gcgcgtctcg ttcgaggtcg
     9961 gcgtcgccga gctcggcggg acgccgctgc tgctgcgcgg cgacgagctg caggtctcgc
    10021 gcggcgagtc cccgcgcgac acggcgctgg tcctgtcgcg cttcgtccac gcgatcggcg
    10081 tccgcaccgg cgaccacgcc ccgctcgagg agctcgccga gcacggctcg atccccatcg
    10141 tgaacatgct cacgcgcgac caccacccct gccaggcgct cgccgacctg ctcaccctgc
    10201 aggagcgctt cggccgcctc gacggcctgc ggctcgccta cgtcggcgac ggcaacaacg
    10261 tcgccgcgtc gctgatggtg ctcggcgcca aggcgggcgt cgacgtagtc gttgccacgc
    10321 ccccgcagct ggcgcccgcg ccggcggcgg tcgagctggc gacggaggtg gcggagggcg
    10381 ccggccgcgc gaccgtcgtc acagacccgc gcgaggccgc cgccggcgcc cacgccgtct
    10441 acgccgacgt ctgggtgagc atgggcgacg accccagcga cgcggcggcc cgccgggtcg
    10501 ccctcgagcc ttaccgcctc gacgacgcgc tgctggccgc cgcgcgcgac gacgcggtcg
    10561 ccctccactg cctgcccgcc caccccggcg aggagatcac cgccgacgtc ctctacgggc
    10621 ctcgctccgc cgtctgggac caggccgaga accgcctcca cgcccagaag gcgctgctcg
    10681 agctgctggt gagctgaccc ggcggcgcag taggctggcc accacacgct cgaaggaggc
    10741 ccgtatgaag cagctcttgg cactgatctg cgcagtcctc gccctcggcc tggtggccgc
    10801 cggctgtgga gacgacgacg acagctcggg cgacggcggc ggcagcgccg cgaccaccga
    10861 gcagaccgca accaccgaga agcaggcctc cggcggcggc gacgcggccg ccaagcaggt
    10921 cgagatcgag gtcgtcgaca tcgactacga gcccaaggac gagacggtcc cgagcggcac
    10981 caccgtcaag tggacgaaca ccggcgacct cccgcacacc gtcaccaagc aggacggccc
    11041 cggccccgac ttcgactccg ggaccatgga gcccggcgac acctacgagg agaccttcac
    11101 cactcccggc aagatcgcct accactgcac gatccacgcc ggccaggacg gctcgctcac
    11161 cgtcgagtag ccatcacata tcagtgatat tctaggggag tggccccggg tgaactgatc
    11221 cgtacgacgc gcgtccgccg tgggctcagc caagagcgct tggcgcgacg cgccggcacc
    11281 cggcagtccg cgatcagtcg gctcgagcga ggcgacgtgt cgcccaccgt agagacgctg
    11341 gagctgcttc tcaacgcgat gggtgagcga ctggagctgg acggggcgcc actcgagcgc
    11401 cgctacgacc cactgcatcg ccgcgccaac gcggcgcgct cgccggagga gcgcctccga
    11461 ctcgcgatct cctggaaccg gatggccggg cgcctcgccg ccgccggccg ggaggcccgt
    11521 ggcgaggcct gagccgctcg acgccgagcg catcctgcgc gcgctcgccg agcaccgtgt
    11581 cgactacgtc ctgatcggcg gcctcgcagt gcagacgcac ggtcacgtac ggaccaccaa
    11641 cgacgccgac ctgatcccgg cgccgaaccc ggccaatctc gaacggctgg cggccgctct
    11701 gagagcgctc gacgcgcgcg tgctcaaccc cggcagcgag gacgtgccga tcgacgcgcg
    11761 catgctcccg cgcgccacca tctggcagtt cgcgacgccg gacggtggtg tcgacgtcat
    11821 gcatgaggtg ccggacggcg atcccttcga ggagctcagc aagcgcgccc tgcgcgtccg
    11881 tctcggggat gtggaggtgc ccgtcgtgag cctcgacgac ctgatcagga tgaagctcgc
    11941 gcgagggcgc agcatcgatc tcgaggatgt ggctgcgctg accgatcctg catgacgttg
    12001 cggtggctgt gatctagaac cggcacgcgt gcgggccgcg gagggttaga ttgcgcttcg
    12061 catgtcggcc tactacccgg gaccgaatcc gccggacgct gtcgagctgc gcgtgctcgg
    12121 cgcgctgatc gagaagcggc ggacgactcc ggacgcctat ccgctgagcg tcaactcgct
    12181 gcggctggcc tgcaaccagg cgacgaaccg cgacccggtg gtcgagtacg acgagcggac
    12241 ggtgctcgac gcgctcgagc ggctggggcg gcgcggctgg acgcggctcg ccagcggcgc
    12301 aagcagccgc gcgcgcaagt accgccacct cttcgacgag gcgctcggcc tcgtcgacga
    12361 cgagctgtcg atgctcgccg tgctgatgct gcgcggtccc cagacgccgg gcgagctcaa
    12421 gcagcgcacc gaccgcctgc acccgttcgc cgacctcgcc gcggtcgaag acacgctcga
    12481 gcggctgatc gatcgcgagc tggtcgcccg cctcccgcgc cgccccggcc agaaggagca
    12541 gcgctacgtc cagctgctcg acggcggaga cgacgaggca cccgagcggg tggccgcgcc
    12601 ggccacgccg gtcacgacgc ccgcccctcc cggcttcgat ggcgctcccg cgcctgtgga
    12661 cgtgccaccc gcgggcctgt cgtccgagct cgaggagcgg gtcgacagcc tcgagcgcca
    12721 ggtcgccgag ctgagcgcgg agctgcgcgc cctgcgcgcc gagctcggcc cctagctcgt
    12781 cttcgccgcc gccctcgcct cgacggtcgt caccggcagg ccggtggcgt gcgccgcctt
    12841 gcgcggcagg tcgagatgca gccacttcga gatgtggtgc ggcagcgtcg agacgatcag
    12901 ctcgtcgtag ctgccgcgat tgcacgcgtc ctggatcgcg ccgagcgggt cggggtcgcc
    12961 gacgatcgcg tcctcgacgt ccacgcccgc gtcgcgcagc cggccgatca gcgcgtcgcg
    13021 gtgacgctcg gcctcccgct tcccgacctc ggcgtccatg ttcgccgccc aggacagccc
    13081 gtgcggcgtg gcggggatca gcaccgtgaa ctcgcagtcc ccctggccgt cacgctcccg
    13141 caacgccgtc agcagctcct ccgactcggc ggtctggttc gccaccacaa gcaccttcgc
    13201 cacggctcct cctccgttct ccctaaggtc agagttaccc gtccggcacg tctgccaatc
    13261 gacggccttt ctccccttcg ataaggtcgc tgcgtccaac ggctggaggg ttgatggcat
    13321 cgagtgggtt cagggctccg tcgcgtgggc gactggcgag cctcgcatgg gtgacgctgg
    13381 tcgtggcggc gggtctcctt caatcggcgg cggcggcgtc gggggcaacc ctctcaggga
    13441 cggtcagcgg gcagtccgga gacgggcagc gacagcccgt gtccggcgcg ctggtgcaag
    13501 tgatcgaccc acagagcggt gacgtggtcg ccgtcggcac gacgggcttc gcgggcgagt
    13561 actcgatcgg cgtcgcaccg tccacctatc gcgtgcgctt cgatcctggc ccagggcagc
    13621 tgtggggatc cacgacggtc gacgacatcg acgtcagcag cgatcgggtc ctcgacgtcg
    13681 tcctgatcct gacgggcttg gttcgcgtga gcggggtggt gcgtgacgct gccggcctgc
    13741 cggtcgcggg ggtgtcggtc tcggccgcgg gcgggtctgg gaggacggcc gccgacggct
    13801 cctattcgat caacgcgatt cccggaaccg ggcgcagcgt ggccgtcagc gcgggtggtc
    13861 tcgccctgct cgggctgccc gagagctggt ctctgcggtc gtcgccattc gagttgcagt
    13921 ccgatgccgt tcgggacttc gtgctgccct ccgcggtgcg cgtcacggtg cgcgtgctgg
    13981 gagtcgagga cgagccgctg gagggcgtgg aggttcgggt cccggagctc gagctcgccg
    14041 gctcgttcgg tggcgtgacg ggtcatgccc gatccggggg cggcaagtac ctgacggacg
    14101 cgaacggcga gatcgcccgc accgtcttcg ggggcgaccc actgagctcg tccagcgacg
    14161 tccggccacc gcagggaagc ggctatggga gcaccaccta cgcgatgccg ccgttcacga
    14221 ccgacacgac ggtcgtggtg cgtccgccgc gcctggttcg ggtgagcggc gtcgtgcgcg
    14281 acgccggcgg cgccgcggtt gcgggcgcac gggtggcggt gggcgacggg atcggcgtgt
    14341 cggcttcgga cggctcgtac tcggtggaga ccgctcccgc agaggctggc aggctgacgg
    14401 tgggagcggg ggcgacggcg ctgcccgggc tgccctcggc gtggtccctg aggtcgtcgt
    14461 tcctcgactt gcggacggac gtcgtgcgcg acgtcacgct gccgtcgacg gcgcatgtaa
    14521 ccgtgcgcgt cctaggggtg gacgacgagc cgttggaggg cgtccaggtt cggattccct
    14581 ccttctcgct cgacggccag ttcggcgacg tcacgggcac cctcgtgtcc ggggccgacg
    14641 gcaagcgctg gaccgatgcc gacggcgagg tctcgacggc gacgttcggc ggcgtccagg
    14701 tgggctcgtc acaggaggtt cgcccgcccg tggggagcgg ctatgggacc acgtcctacg
    14761 cgatgccgcc ggtcaccggc gacacgacct tcgtggtgcg tccgccgcgg caggtccggg
    14821 tcaccggctt cgtccgcgat gctgccggct ctcccgtcgc cggcgcgtcg gtgtcggtga
    14881 ccgacggctc tggcactacg gcggccgatg ggtcctacgc gatcgtcgcc acccctgggg
    14941 ccgcgcgtcg cttgaccgtg tccggtgggc cgtcgatgcc tggctcggtc gtgtcgtcgc
    15001 cgttcgcgtt ggaatcggac acggtcgtcg acgtcgactt ccctccgctc cgcttggtga
    15061 cggttcgcac cctcggggac ggggacgagc cgttggctgg agtggaggtg cgaatcccga
    15121 ccttcgagct cagcgggacg ttcggccagg tcaccgggct tgcgcgatcc ggcggcggca
    15181 agtacctgac cgacgcagac ggggaggtct ccaggctcgt cttcggcggc gagccgcaga
    15241 gctcctccaa cgacgctcga ccgccgcttg gcagcggcta cggcgaggcg acctatgcga
    15301 tgccgacggt caccggcgac acgacggtgg tcctgcggtt ccaaagcgcc aacgccgacc
    15361 tcgtaccgcc ggcgatcgac tgtgccgcgc ccccgggcga ctggcaggcc gacaacgtga
    15421 gcctccgctg cacggcaagc gatctgtctg ccggcctctc cgatccttcg gacgcacact
    15481 tcgacctcgc cactcacgtc cccgagggat ccgaggacgc cgacgccgag accgatacac
    15541 gcgaggtctg cgaccgggcc ggcaactgcg cggtcgccgg gccgatcggc ggcatcaggg
    15601 tcgaccgcca ggcgccgcgg atccgcttcg tgcaggagcc gaacggcttg cagggatggt
    15661 tcgtcgacgc gccgggctcg accaccgccg tagccgacga ctcgacgatc gcctcgctga
    15721 cctgcacccg cgacggcatg cccgtcctcg agacgccggt cgcaggcttc ggctcgctca
    15781 gcctcgtcat gtcggctgcc accgagggca ggcacgcggt cgagtgccgg cgaccgacgc
    15841 ggtcgggcac gcgtccgtcg cgggcgacgc gatcctcatc gacgagtccg cgcctggccg
    15901 gccgaccgta gtcgcagatc ggccgccgga cctcagcagc cgcggcggtt ggtatcgcga
    15961 cgaggtgact gtgtccttcg tccccgacgg cgatcctctg ctcgcggacg ggagcccggg
    16021 tagcggtgtc gatgcgtcga cctcgacaca gccgttgacg atcgacgcca gcggcgtcca
    16081 cgacgtcgtg ggctcggttg ccgacgtggc gggcaaccgc tcggccgacc gctctacgac
    16141 cgtgcgggtc gacgcggatc cgccgacgag cgagctcacc tgcccgcagg atcccccgct
    16201 tggatcgact gcccgagcgc gctgggagga cgacgacggt gagtccggac tcgaccgccg
    16261 ccggcgtgga cagatggtcg tcgacaccgc gaccgtgggc ttccacaccg cgacgcacgt
    16321 agcctccgac aacgtcgggc accagaccag cagcacctgc gtctaccgcg tgatcggcaa
    16381 ctgaacgtcg cgcgcgacgc tagtacggag ggaagtccgc gtaccactcg ccgagcgagc
    16441 ggaaggcggc ccagaacgcg cgcttcgagc gctcgtcgtc gagcgaggcg tcgatgtcgg
    16501 gcacgtagag cgcgtcgcag ctggcggtca gcttctggat cttcccgggc gtggcctcga
    16561 cctcggcggc cagcgggacc tgcagcccgt tgagctcttc gagcgcctcc tcgcccatcg
    16621 cgacgacgat ccgcggctgg acgatcgcca gctcgtcgag cacgcgcgcc ttgcactccg
    16681 gcgccgcgag gtcggtgtcg tggaccgggc acttgacgac cacggtcccg tagaccgcca
    16741 gcgggtcgat tcccagccgc ttgaagctct tcatcagcgc gctgcccgag cggccgtaga
    16801 aggcgacgcc ctcctcgatc tccgaaggcc gcggcgcgaa cttcagcatg aagacgtcgg
    16861 cctgcgggtg gcccgatccc agcaccggca tcaggctgcc gcgggggcag tggtcgcagc
    16921 cctggatctc gtgggtcagc tggttgagct ctctgatcgc ccgctcgagg tacttctcgc
    16981 ggatctcgtc gtcggtcgcg ggcacgaccg agggattaga cggacccggg cgctgtcctc
    17041 ttgcgtgcat cgttcattgc ttagcagccc gcttcgcccc cgcgccgggc ttcgcggtcc
    17101 gccggcgagc ggcgtgcggc gtcgcgcgcg tctggatgaa cttcagcgcc tggacgtaga
    17161 gcagcgactg gcgcttgcgc acgatgtcag cgtcgcgcag cgactcgagg aactcctccg
    17221 gcccgtcgaa cgcccgcatc cggatcttca ccgagcccag gccggccgcc acatggctgt
    17281 ccgaccccgc ccccgcgacg atccggtact tctgcgcgaa gcgcgccgcc tcctcgttga
    17341 acgccgagaa cgccacgcgc gggttgaaca cctcgatcgc atcgatctct tcgacgacgt
    17401 cgagcagatg ctcgtagtcg ggcaccgagt gcatccggtc gaacggatgc ggcacgtaga
    17461 ccagcccgcc ctgcgcccgg atcgccgcga tcgtctcctg cagcgtcatt cccttcggga
    17521 tcttctcctc gatgaacagg ccgatcacct cgccctggtc ggcggtcttg acctcctcgg
    17581 ccacgatcac ctggatcccg cccatctcct ccgccagcac ccgcgcctcg tgcgcccccg
    17641 acacctcgtt gtggtcggtg atcgcgatcg cgtccagccc cgccgccttc gccgcctcca
    17701 gcaacgtcgc cgccggcgtc gcgcagtcgc tcgagtggtc ggtgtgcatg tgcaggtcga
    17761 cgtgcacgaa ctcccggtcc gccagccgct tccgcaccgc cggcttcccg tccggatcgt
    17821 gccgccgcgc cgccgctgtc gcatagatcg cctcgaactc gtcggccgcc ctcgaccacg
    17881 acagcccctc acgcgccgcc gccacccgcg ccgcgaaccc ctcgcgcagc tccggctcgc
    17941 tcaccagccg ctcgagctga gcgcccaacg ccagcgcatc gcgcgggtcg aacagcagcc
    18001 ccagctcccc atcgtcgagc agctcctcgt actgcggcag gtttgccacc accggcaccg
    18061 ccccagcccc cagcgcccgc atcaccagct gcgaagacgg cgcaacccca gtcgacgcgg
    18121 caacgaccac atccgcccgc gccaaatgct ccgcctccgt cccatcggcc gggcccgcca
    18181 tccgcacccg atcccgcaat cgccgcggca gctgcaacgg cgacgccacc gccgccggat
    18241 cacgcaacca gatcgtcgcc cgccagtcgc cgtccaccct cagcttccgc agcgctcgga
    18301 cgaacagccg caacgccccc cgatcctcat ccgccgaata gaggatctcc accggcccac
    18361 cctcgcccgc ccgctcgatc agctccgccc ccgacgcaac gacaccgtaa tgccccggaa
    18421 agaaccgctc caccacatcc cgcgccgtcg ccgacgtcgc cgtccgagca tccaaccgcc
    18481 cgaacaacaa ctgcaccacc cgccgcgcca cctgcgtcga caggaaccgc tcactcgtcc
    18541 catggaacgt ccccacgttc agagtccgcg aatgcctcag cgccgcgcca gccgcactcg
    18601 gcgcaaacgg ctcatgcaca tgcacgaagt ccagctccgc atgctccagc agatcctcca
    18661 acgtccgcga cacatcgatc ggcagggaga ccgcccgccc cctccgaaac ggcaggctct
    18721 gcccgaccgc cagcacgtcc ccatcgaaca gcgcgccttg gtcgccccgg accacgcgct
    18781 tcacccgagc acgaccgtcg cggatcaagc ttcgcgaatc ggagggcgcc accacgacga
    18841 ccctgtggcc gcgctcacgc agctcgctgg cgacccgctc cacatagcgg ttcacgtcgt
    18901 gcccctgctc ccaggggtac ggcgtcacct gcgcaatcgc aaaccgctcg tcagacacgg
    18961 cgacgcagga taagaccccg cgtcgccgtg gcccggctac gcctcagtcg gctcgctgct
    19021 cacaggcgtc ccacccggca gcttcggcgc cccaccgttc tcagcgatga acgcctccac
    19081 gttctcacgc gcaaccgagt ccggcacctg caccggcggc gacttcatca gatagctcga
    19141 aggcgcctcc agcggccccc cgatgccacg attcaaagcc agcttgcagc accgcgtcgc
    19201 atcgatcacg atccccgccg agttcggcga gtcccacacc tcgagcttca gctccacgtt
    19261 cagcggcacg tccccgaacg cctgcccctc gacccggatg tgcgcccact tgcggtcggt
    19321 cagccacggc acgtagtcgg acgggccgat gtagacgtcg tccttgggca gctcgtggtc
    19381 catgatcgac gtgaccgcgt tggtcttgga gatcttcttg gactccagcc gctcgcgctc
    19441 gagcatgttg tagaagtcca tgttgccgcc gacgttgagc tgcgacgtgc gcatcatctt
    19501 cacgccgcgg tcgccgaaca gccgcgcgag ctggcggtgg acgatcgtgg ccccgacctg
    19561 cgacttgatg tcgtcgccga cgatcggcag gccggccttc tcgaagcgct tgccccagta
    19621 gtcctcgcgg gcgatgaaga cggggatgca gttgacgaag ccgcagccgg cctcgagcac
    19681 ctgctcgacg taccacttgg tggcgagctc cgacccgacc ggcaggtagg agacgacgac
    19741 gtcggtcttg gtgtccttga ggatctggac gatgtcggcg gtctcgcccg gcgccttctc
    19801 gatcttctgc ttgaggtact tgccgaggcc gtcgtgggtc atgccgcggt ggacgggaac
    19861 gtcgaggtgc ggcacgtcgg cgaacttgat cgtgttgttc tggccggacc agatcgcctc
    19921 ggagaggtcc ttgccgacct tgtcggagtc gatgtcgaag gcggcggtga actcgatgtc
    19981 gccgacgtgg tagccgccga ggtcgacgtg catcaggccg gggacgtcct cgtcgggatt
    20041 ggcgtccttg tagtactcga cgccctggac gaacgccgag gcgcagttgc cgacgccgac
    20101 gatcgcgacg cgcaccttct cggtctggta gcgcgacgcg ccgttggttg aaacgttttc
    20161 gctcactgag cctccgggat ctgaggaaga agaaggggaa ggggtgggcc cctgccggga
    20221 ccggcacgcg ggaaagaacc ccgaacgccg gttcgtgtta catccgcgca gcgccctggg
    20281 aggcgcgcga acggtccgct gaacggtaac ggctggcgtt caggcgagcg tggcggccga
    20341 cccgttgagc gcccggcgga cgtgcaggat gcgctggaag gtggtgatca cggtcagcgc
    20401 ggcgagcacg tagatcgcgg gcgcgagcag ctcgaagtcg ccgagcccgg cgcccttggc
    20461 gaacagcagg ccgaccgaga ggatcacgac gcggacggcg cgggtggcga ggccgacctt
    20521 gcactcgacg ccgagcgcct cggcccgtgc gcgcgtgtag gagaccatca gcgagaacag
    20581 gaccgcggcg actacagcgg cgaccgcgag atcatcgccg cgcgaggcga agtaggcgcc
    20641 gatcgcggtc agcacgatgc cctcctcgac gcggtcgagc gtcgagtcga ggaatgcgcc
    20701 gaacggcgtc cccttgcccg acatccgcga gtagcggccg tcgagcgtgt ccatcaccga
    20761 gccgaggatg aaggcgatcc cggcgaggaa gaagtagcgc tgggtgacga gcaccgcggc
    20821 gatcacgttg ccgacgagcc cggtcatcga gatcgcgttc ggcgtcaggc gcgactcgat
    20881 cagacggttg cgcgcgagcg cccgcatctc ggcgctgcgg accgccacta ggccaccgct
    20941 cgcgcgcgcc ggtcctgcca cgcccagacg tcgggccgca cccggccgag cgcgtaggag
    21001 gcgccccagg cggagatcgc cgcgaccagc gccccgagcg ccgcatcgag ccagaagtgg
    21061 ttggcggtca cgacgacgac gaacgtgacg accgccgggt agagcgccca gaggaccttc
    21121 gcgaagcggt tctggacgat ccgcatcgcc gggatcccga ccatcagcgc gaacgcgacg
    21181 tgcatcgacg ggacggcggc gaacgggttg tagaggacgt tcgcgctagc ctcggcctgc
    21241 ggcccgacga acgccgagac ggtgtcggtg aagccccact ccggcatgta gcgcggcggc
    21301 gcggtcgggt aggccatgta gccgacgagc gcgaggccca tcgcgatcat gaacatgttg
    21361 cgcacgtagt agtacgagga gttgcggaag atgtagagcc agatcaggaa cgtcgtcgtg
    21421 atcgtgaagt gcgagttgac gtacatccag ttcgcgcccg tcacgatcca ctcctcgccg
    21481 agcgcccacg cctgcagccc cggctcgaag aacagcccga gcgtccgctc gatgtcgacc
    21541 agtgtgcgcg cgttctcgaa cgccagcgtc gcccggccgt cgaccagccc gcggacgatc
    21601 cggtagacgt agtacgcccc ggcgaacagc gcgagctgac gcagcgcgtc gagccagccc
    21661 tgcggcagcc agcggctcgt gacccgccag gcgcgtcgga actggagcgt gaagtcgtcg
    21721 tagacctggc gcacgatcag ggcagggtac cgcgcctagc tgctgttgca gcgcgggccg
    21781 cgctggaaga tccgctcggg cgagaagcgg gtgacgagcg gcaggaagcc gggcttctcg
    21841 gcggtcacgc gcgtccactc gccggcgcgc tggacgcagc cgcccttggc gagccagtac
    21901 ggcgtccagc gcacgcggac gagcgcgctg ccgggacggc gcacacgcag cagcacgcgg
    21961 tcggagccga gctgctcgag ctggatcttc gcgtcgccct cgg
//
LOCUS       DATABV010000218         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1197374, whole genome shotgun sequence.
ACCESSION   DATABV010000218 DATABV010000000
VERSION     DATABV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197374"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..371)
                     /locus_tag="VHF88_10140"
     CDS             complement(33..371)
                     /locus_tag="VHF88_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2162164.1"
                     /translation="MTLLQIEAQEHDGLAHVVLTGELDLSTIAQVEQELSRVEGEGPA
                     TVALDLSRLTFLDSSGLRVIVSADQRARRENRRFVVVRGPDTVQRVFSITRLDEQLDL
                     VDDFGDLTQS"
     gene            complement(381..764)
                     /locus_tag="VHF88_10145"
     CDS             complement(381..764)
                     /locus_tag="VHF88_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX2162165.1"
                     /translation="MDGIDVAGLLAIGFCAGMLGGLVGVGGGVLFVPGLVFFLGLSQL
                     DAISTSLVAVVFVGVVGAWRQRGYGNVRVRDGLIVGALSPLGVLAGTVLANAVSERAL
                     ELSFAAVQVFFAWRLASRALRPRNG"
     gene            complement(764..1120)
                     /locus_tag="VHF88_10150"
     CDS             complement(764..1120)
                     /locus_tag="VHF88_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2162166.1"
                     /translation="MASRLPKLVVIATVGGAFSGLFGVGGGTVIVPLLILWFGYGERE
                     ATGTSLLAIVVIAALAATAQGSYGNVHLKEGVLIGIPAVAGVVAGTALQQRIPERAVA
                     AIFSVLLVVSAAVLVF"
     gene            1141..1953
                     /gene="otsB"
                     /locus_tag="VHF88_10155"
     CDS             1141..1953
                     /gene="otsB"
                     /locus_tag="VHF88_10155"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX2162167.1"
                     /translation="MSAPTAETLRESLRPLTEDPGRSAVFCDIDGTLAPIVRRPEEAH
                     VREEISLLLSQLGRRYGCVACVSGRPVAEARRLVGVGSIAYAGSHGAELLHAGEKKPT
                     LAPAFASWEGRVRRFVSARENVRELKLLRIRIEDKGPIKAFHWRDVPDEPAAESWLAN
                     VAQEAETEGFATHWGRKVLEIRPPVPIDKGQAVRTLVEEARVGCALFAGDDVTDLDAY
                     DALDALVGAGTLAGAVRVGVRSAEGPPAIVERADVVVDGVRGMVDVLAALAD"
     gene            1954..2493
                     /locus_tag="VHF88_10160"
     CDS             1954..2493
                     /locus_tag="VHF88_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162168.1"
                     /translation="MLFRDFLRVAVMLFGGAGTALAVVAIAGASGEDSTLPAYVAVGW
                     WVVAAAAGLWLGRRNAPTQQIGRLLASARNTNMLPEVEPGAIVFNRLWPLAAITVVSG
                     GVAFLLPQIPAIAAGYAIAVALTWRKQARAVQAIEERDGVQFYFDRTPPFGAPKLIRA
                     PGLRKVEPAQDPAPAVKPL"
     gene            2541..3434
                     /locus_tag="VHF88_10165"
     CDS             2541..3434
                     /locus_tag="VHF88_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162169.1"
                     /translation="MVGGTIRRRLVVSVCALALGPVAAACGSDGDDGTAKSEPRIAAA
                     SEPEDVFIERMAKLLETTSAKKDCAQLEEINVRSFTRFPCPPAKDLRKSMASFEVVDT
                     EDYGTGAVIDYKSGKAEDGAAIVLFVAPDRNWGIGRFGLITEPSIGTSDEESRSGYAE
                     AVDKYLDAVRDRDCAAFKEVTFHADADQAEICETSFAGTEDLAKRLKADPSAEPIYQG
                     GNRTYGFFTLETSKPVPPENSTISVIRAAGDSYLILDVAPSPTADEQRRVRQQLREQQ
                     RKGGPSSMEPSSKPSDPAVRQ"
     gene            complement(3424..>4969)
                     /gene="polX"
                     /locus_tag="VHF88_10170"
     CDS             complement(3424..>4969)
                     /gene="polX"
                     /locus_tag="VHF88_10170"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX2162170.1"
                     /translation="PGIGKTIAEKIDALLLDGEIPAAVKLKERIPPGLIKVTRIPGLG
                     PKRARQLHDALGIETIDQLREAAEAEQIRTVPGFGAKAEEKILAALAAGADGSVQARV
                     LLSTARAVGDEMVACLREHPAAVQVELAGSARRWADTCKDLDVVAATDDPQALSDGFA
                     ALPQIDDVTTSGTAGAKAITHQGLAVDLRIVTPDRFGNLLQHFTGSGRHNEALRTAAV
                     KQGLHVSEYGILDDATGETLSCATEAEVYERLGMAYIKPELREDRGELAAARAGELPD
                     LIELNDIRGDLHCHTTASDGHQTVLEMARAAQARGYEYLAITDHSASHGFGNHVSPGE
                     LVRQIERVAEANEQLGDDFTVLSGSEVNILPDGTLDYSDELLAQLDWVIASAHTSFRM
                     SEDDMTKRIVTAIENPLVRAIGHPTGRMLGRREPYAVDMDRVIEAAARTGTFLEINAN
                     PNRRDLHDVHARAAAAAGVKLVIDSDAHRAETLAVIRYGIATARRAWLTADDVANTRP
                     WSELTA"
BASE COUNT          723 a         1715 c         1831 g          700 t
ORIGIN      
        1 cggcggtccg gcgtggaagc cggaggagtc cgctaggact gagtgaggtc gccgaagtcg
       61 tcgacgaggt cgagctgctc gtcgagacgg gtgatcgaga agacgcgctg gacggtgtcc
      121 gggccgcgca cgacgacgaa gcgccggttc tcgcgccggg cgcgctggtc ggcgctgacg
      181 atcacgcgca ggccgctgga gtcgaggaag gtcaggcgcg agaggtcgag cgcgaccgtc
      241 gccggcccct cgccctcgac ccggctgagc tcctgctcga cctgcgcgat cgtcgagagg
      301 tccagctctc cggtgaggac gacgtgggcc aggccgtcgt gctcctgtgc ctcgatctgt
      361 aggagggtca cgggcgcaac ctatccgttc cgcggccgca gcgcgcggct cgcgagcctc
      421 catgcaaaga agacctggac ggccgcgaac gacagctcca gcgcgcgctc gctgaccgca
      481 ttggccagca ccgtcccggc cagcacgccg agcggcgaga gcgcgccgac gatcagcccg
      541 tcgcgcacgc gcacgttgcc gtagccgcgc tggcgccagg cgccgacgac cccgacgaag
      601 acgaccgcga ccagcgacgt cgagatcgcg tcgagttgtg acagcccgag gaagaacacg
      661 agccccggca cgaacagcac gccgccgccg accccgacca gcccgccgag catcccggcg
      721 cagaacccga tcgcgagcag gcccgcgacg tcgatgccgt ccatcagaag accagcaccg
      781 cggccgagac gacgagcagg accgagaaga tcgccgccac cgcgcgctct ggaatccgct
      841 gctggagcgc ggtcccggcg acaaccccgg cgaccgccgg gatcccgatc aggaccccct
      901 ccttgaggtg gacgttgccg tagctgccct gggcggtcgc cgcgagcgcg gcgatcacga
      961 cgatcgcgag cagcgacgtg ccggtcgcct cgcgttcgcc gtagccgaac cagaggatca
     1021 gcagcggcac gatcacggtc ccgccgccga cgccgaacag cccgctgaac gcgcctccca
     1081 ccgtggcgat cacgacgagc ttcggcagcc gagacgccat cgcgaggcat actacgtcca
     1141 gtgtccgcgc ccacggcaga gacgctgcgc gagagtctgc gcccgctgac tgaggacccc
     1201 ggccggtccg cggtcttctg cgacatcgac ggcacgctcg cgccgatcgt ccggcggccc
     1261 gaggaggccc acgtgcgcga ggagatctcg ctgctgctga gccagctcgg gcggcgctac
     1321 ggctgcgtgg cgtgcgtgtc ggggcggccg gtggcggagg cgcggcggct ggtgggggtg
     1381 gggagcatcg cctacgcggg ctcgcacggg gcggagctgc tgcacgcggg cgagaagaag
     1441 ccgacgctgg cgccggcgtt cgccagctgg gagggccgcg tgcgccgctt cgtcagcgcg
     1501 cgcgagaacg tgcgcgagct gaagctgctg cggatccgga tcgaggacaa ggggccgatc
     1561 aaggcgttcc actggcgcga cgtgccagac gagccggccg ccgagagctg gctcgccaac
     1621 gtcgcccagg aggccgagac cgagggcttc gcgacgcact ggggccgcaa ggtgctcgag
     1681 atccgcccgc cggtgccgat cgacaagggg caggccgtgc ggacgctggt cgaggaggcg
     1741 cgcgtcggct gcgccctgtt cgccggcgac gacgtcaccg acctcgacgc ctacgacgcg
     1801 ctcgacgcgc tggtgggcgc tgggacgctc gcgggcgccg tgcgggtcgg cgtccgctcc
     1861 gcggagggcc cgccggcgat cgtcgagcgc gccgacgtgg tcgtcgacgg ggtgcgcggg
     1921 atggtcgacg tgctggcggc gctcgccgac tagatgctct tccgcgactt cctacgcgtg
     1981 gcggtgatgc tgttcggcgg ggccggcacg gccctggccg tcgtcgcgat cgccggagcc
     2041 agcggcgagg acagcacgct gccggcctac gtcgccgtcg gctggtgggt cgtcgcggcg
     2101 gccgcagggt tgtggctggg gcggcgcaat gcgcccaccc aacagatcgg tcgcctgctc
     2161 gccagcgcgc gcaacacgaa catgctgccc gaggtcgagc cgggggcgat cgtcttcaac
     2221 cgcctctggc cgctcgccgc gatcaccgtc gtgtccggcg gcgtcgcgtt cctgctgccg
     2281 cagatccccg cgatcgccgc cggctacgcg atcgccgtcg cgctgacgtg gcgcaagcag
     2341 gcccgcgccg tccaggcgat cgaggagcgc gatggcgtcc agttttactt cgaccgcacg
     2401 ccgccgttcg gggcgcccaa gctcatccgc gcgccggggc tgcgcaaggt cgagcccgcg
     2461 caagaccccg cgccggccgt caaacctctg taggctcggt cccaggaggg agttcgcgaa
     2521 gccaggaggg agctttgacg atggtcggag ggacgattcg acggcgactg gtcgtgtcgg
     2581 tctgcgcctt ggcgctgggc cccgtcgcgg cagcctgcgg cagtgatggc gacgacggca
     2641 ctgccaagag cgagccgcgg atcgcggcgg cgagcgagcc ggaggacgtc ttcatcgagc
     2701 ggatggccaa gctgctggag actacgtccg ccaagaagga ctgcgcgcag ctcgaggaga
     2761 tcaacgtccg ctcgttcaca cggttcccgt gtccgcctgc gaaggacctg cgcaagagca
     2821 tggcgagctt cgaggtcgtc gacaccgagg actacggcac gggcgcggtg atcgactaca
     2881 agtccggcaa ggccgaggac ggcgccgcga tcgtcctctt cgtcgcgccc gaccgcaact
     2941 ggggcatcgg acgcttcggg ctcatcaccg agccgagcat cggcacgagc gacgaggaga
     3001 gtcgctccgg ctacgcggag gctgtcgaca agtaccttga cgcggttcgc gaccgcgact
     3061 gtgccgcgtt caaggaggtg accttccacg ccgacgccga ccaggcggag atctgcgaga
     3121 cgtcgttcgc cgggaccgag gacctcgcca agcggttgaa ggcagacccg tcagccgagc
     3181 cgatctacca gggtggcaat cggacgtacg ggttcttcac cctcgagacg agcaagcccg
     3241 tccctccaga gaactcgacg atcagcgtca taagggccgc cggcgactcc tacctgatcc
     3301 tcgacgtggc gccgagtccc accgcggacg agcagcgcag ggtccgccag cagctccgcg
     3361 agcagcagcg caagggcggg ccgtcgagca tggagccgtc gtcgaagccc tccgatcccg
     3421 cggtcaggca gtgagctccg accagggccg tgtgttcgcc acgtcgtccg ccgtcagcca
     3481 ggcgcggcgg gccgtggcga tcccgtagcg gatgacggcg agcgtctcgg cgcgatgggc
     3541 gtccgagtcg atcaccagct tgacgccggc ggctgccgcg gcccgtgcgt ggacgtcgtg
     3601 caggtcgcgg cggttggggt tcgcgttgat ctcgaggaag gtgcccgtgc gtgcggcggc
     3661 ctcgatgacg cggtccatgt cgacggcgta gggctcgcgg cggccgagca tgcgaccggt
     3721 cgggtggccg atggcgcgga cgagcgggtt ctcgatcgcc gtgacgatcc gcttggtcat
     3781 gtcgtcctct gacattcgga acgaggtgtg ggcactggcg atcacccagt cgagttgcgc
     3841 gagcagctcg tctgagtagt cgagcgtgcc gtcgggcagg atgttgacct cgctgccgga
     3901 gaggacggtg aagtcgtcgc cgagctgctc gttcgcctcg gcgacgcgct cgatctggcg
     3961 gacgagctcg ccgggagaga cgtgattgcc gaagccgtgg cttgccgagt ggtcggtgat
     4021 cgccaggtac tcgtagccgc gggcctgggc ggcgcgggcc atctcgagga cggtctggtg
     4081 gccgtcggag gcggtcgtat gacagtgcag gtcgccgcgg atgtcgttga gctcgatcag
     4141 gtcgggcagc tcgcccgccc gtgccgcggc caactcgccg cgatcctcgc gcagctcggg
     4201 cttgatatag gccatcccga gccgctcgta gacctcggcc tcggtcgcgc agctcagcgt
     4261 ctcgccggtc gcgtcgtcga ggattccgta ctcgctgacg tgcaggccct gcttgaccgc
     4321 agcggttcgc agcgcctcgt tgtggcggcc cgagccggtg aagtgctgga gcaggttgcc
     4381 gaagcggtcg ggggtcacga tccgcaggtc gaccgccagg ccctggtggg tgatcgcctt
     4441 cgctccggcg gtcccgctcg tagtcacgtc gtctatctgg gggagcgcgg cgaacccgtc
     4501 gctcagcgcc tgagggtcgt cggtggcagc gacgacgtcg aggtccttgc aggtgtcagc
     4561 ccagcggcgc gcgctgcctg caagctcgac ctgcaccgca gcggggtgct cgcgcaggca
     4621 cgcgaccatc tcgtcgccga cggcgcgcgc ggtcgagagc agcacgcgcg cctggaccga
     4681 cccgtcggcg cccgcggcga gtgcggcgag gatcttctcc tccgccttcg cgccaaagcc
     4741 gggcaccgtc cggatctgct cggcctctgc cgcctcgcgc agctgatcga tcgtctcgat
     4801 cccgagcgcg tcgtggagct gacgcgcgcg cttcggcccg agacccggga tccgggtgac
     4861 cttgatcagc cccggcggta tccgctcctt gagcttgacc gcggccggga tctcgccgtc
     4921 gagcagcagc gcgtcgatct tctcggcgat cgtcttgccg atccccggc
//
LOCUS       DATABV010000219        13720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_314435, whole genome shotgun sequence.
ACCESSION   DATABV010000219 DATABV010000000
VERSION     DATABV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314435"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VHF88_10175"
     CDS             <1..1100
                     /locus_tag="VHF88_10175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2162171.1"
                     /translation="VDELYEIAHSRALSVAPVYGGVGIQAQARKAAKAHIVVATPGRL
                     EDLIQRRELSLEHVQVLVLDEADRMLDMGFKPAVDRIVGQTPDDRQTMFFSATLEGGT
                     GQVAQSYTRDARRHVHTPKVERKADVQHRFLHVAHADKVDTLIRELNETERGRTLVFV
                     RTKRGADRLVQRLGKSGIKAVAMHGNKSQNQRQRALANFESGKVDTLVATDVAARGID
                     VPDVTHVINYDAPEDRDTYVHRVGRTGRAGATGAGVSFVIGDQVRDMRRIAQDLGLLS
                     EFDHEAPAHVTGPPREMAARGNGGHRGQGQKRGGGKGRGRSNDGQGRGRVSESRDGTG
                     GNRPKPRPNSAGTARGNVASSRPPRKRRSRV"
     gene            1122..2555
                     /locus_tag="VHF88_10180"
     CDS             1122..2555
                     /locus_tag="VHF88_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX2162172.1"
                     /translation="MTESRERLDRSTVVALAAMAMAVFVIANDVTALSVALPEIECDL
                     DASVGTVQWVISAYALIFGVLIVTGGRLADLLGRRRVFFVGAAIFAVFSVLGAVAPSA
                     LTLILARGLMGIGGALMWPAILGMTYSILPEKRAALAGSLIIGAAGFGNAAGPLIGGF
                     LTDALDWRWILALNLPIAAVASLVTWRYVPESSGDDAERRFDYAGVATLSVGLIALLV
                     ALDEVTDLGWTDPLVLGLFALCVVLLIAFAASERRAGSWALVPDDVIRNRAFRSACLA
                     VLLMSPTFFVALMYLPQFMQKVLAYTPVEAGLGLLPMMGVFAVVSFAAGPLYERFGPR
                     PVIAAGASCMVAGPLLISLIERGDSWAQLVPGMAVLGLGVGLFYSSITTAAVTALDSS
                     RSSLAGGIGYMFQVAGGSIGLGLATTVFTEAVERGGFEDGFQWAFRLVAALALVALAV
                     VLLYVGRAEDYEADDDSSAELASGSSA"
     gene            complement(2501..3613)
                     /locus_tag="VHF88_10185"
     CDS             complement(2501..3613)
                     /locus_tag="VHF88_10185"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEX2162173.1"
                     /translation="MAAPSDDETQERARAALIERLRALGQSEQAIAAAEQEDRLATFA
                     VEIALGGDRRHTLTGVAKLSGLSPAFLRRLMQASGRAAALPRERAYTDDDVELARRIK
                     LFADAGIPREGLLETSRVIGQAMSQTAEAIRRTTVSPLVRPGDTDLTLALRYVEAAQA
                     LAPEVAFVLDSAFRTQLREGIRSDLVAEAERDAGRLAGTRDIAIAFADLVGYTKLGEQ
                     LPAERLGQVATRLAELATDSICRPTQLVKTIGDAVMLVSWEVDPMLETLRSLVAATEA
                     EGEEFPPLRVGVAFGPATPRGGDWFGSTVNLASRVTDAGKPNRIYATEPVVELAGGGY
                     GWKRSRRRNLKGIDGRTRLYALLPEASSADESSSAS"
     gene            complement(3654..4931)
                     /locus_tag="VHF88_10190"
     CDS             complement(3654..4931)
                     /locus_tag="VHF88_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2162174.1"
                     /translation="MSATDSVAELTGDGTGPEAGETPFSLELSQDQKDIRDWVHGFAE
                     NVVRPAASEWDEREETPWPVIQEAAKIGLYGFESLAQFFMDPTGLQMPIVNEELFWGD
                     AGIGMSIMGTSLAVAAIFGQGSNEQIGEWIPQCFGSQDEPAVAAFCASEPDAGSDVSK
                     IRTTAKYDEASDEWVINGQKAWATNGGIAKVHVVIASVDRELGSRGHAAFVIPPGTKG
                     CEQGAKVKKHGLRASHTADVHLDDCRVPGSCLLGGKEKLDERLARVREGKQARSQAAM
                     QTFEASRPTVGAQAVGIARAAYEYALDYAKEREQFGKKIVENQAIAFTLADMKMEIDA
                     ARLLVWRASWMGKQSGMVFENAEGSMSKLKAGEVAVWSTERAIQILGGNGYTREYPVE
                     RMHRDAKIYTIFEGTSEIQRLVISRAISGMKIS"
     gene            5055..5228
                     /locus_tag="VHF88_10195"
     CDS             5055..5228
                     /locus_tag="VHF88_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162175.1"
                     /translation="MAENEGQQKTPDVQRASSTSPLDGRTDKATKPPGQGEIDREAVD
                     KGWEALDRAGGGH"
     gene            5303..7321
                     /locus_tag="VHF88_10200"
     CDS             5303..7321
                     /locus_tag="VHF88_10200"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX2162176.1"
                     /translation="MTHADAPQVLWQPSEELKQGSALAAYIEWLKVDRGVAIETYDEL
                     WRWSVDDLDGFWASIWDYFDVRASAPYDAVLGSRAMPGAEWFPGAQLSYAEHVFRDRE
                     DSAVAIRHASELRGLSELSWGQLRSLTARIAGGLRDLGVGPGDRVVAYLPNIPETIAA
                     FFACASIGAVWSSCSPDFGVRSVVDRFAQIEPRLLFAVDGYRYNGRDFDRIDTIAALQ
                     REIPSLEHTVLLPYLDSAADAGRLAGGITFPELLERGEDTPLEFAQLPFDHPLWVLYS
                     SGTTGLPKAIVHSQGGILLEHLKKLNLHLDARPGDRVFWFTTTGWMMWNFLAGVLLTP
                     ASIVLYDGSPAHPDLGVLWDLAERAGVTCFGTSAAYIASCMKGGVEPGEGRDLGALRS
                     VGSTGSPLSPEGFGWVYDHVGSDTWLFSTSGGTDVCTAFVGGCVLLPVYVGELQGRSL
                     GADVQAFDEDGNAVVGEVGELVISQPMPSMPIFFWNDPGGERLRESYFSMYPGVWRHG
                     DWIEITERGTAIIYGRSDSTINRGGIRMGTSEIYRAVLAVDEIVDALVVDVPASQSAA
                     ARAAGVAEDDSWMPLFIVLRDGATLDDDLEREIARRVRAECSPRHVPSGVYAIAEVPR
                     TLSGKVLEVPVKRILMGTPPEQAAARDSLQNPAALDWFVELASGHDGA"
     gene            complement(7335..8192)
                     /locus_tag="VHF88_10205"
     CDS             complement(7335..8192)
                     /locus_tag="VHF88_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX2162177.1"
                     /translation="MADPLLLVDAPYLLYRAFFGLPDKIRGADGRPVNALLGSVNTIL
                     AQAEERGARAVVCCFGPDAAPYRVNLYPGYHAHRPPVPDDLAHQWAQAPRLYGALGWQ
                     ITDTDELEADDLLGAYARVEQQAGGEALVLTGDRDMFQCATDSVRVLLLGGRGKKGPE
                     EIGPPQVRERYGVEPEQVPDFIALRGDPSDGLPGAKGIGEKRAADILRRHGSLEAALA
                     EADRDGPDANSLCRQADQLRAFREIATLRPVDVERPPDAPTDRAGGAAAAAELGMKRL
                     AERLAAAGD"
     gene            8317..8520
                     /locus_tag="VHF88_10210"
     CDS             8317..8520
                     /locus_tag="VHF88_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2162178.1"
                     /translation="MPGGTVKWFSDEKGFGFITPDDGGKDLFVHHTGISGDGFKSLTE
                     GARVSYDAEQGDKGPKAVNVIQS"
     gene            8613..9182
                     /locus_tag="VHF88_10215"
     CDS             8613..9182
                     /locus_tag="VHF88_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2162179.1"
                     /translation="MPSIQLAQDEHIVFEGHPSWRATLAFYIKGILGAIVLLAAFAGI
                     ASLFADGVDTTTLAIVGLVLLVLVLVGGYVWRMATTYTITNRRLHIKRGIVARKTQEA
                     RLERVQNVNTDQSVLGRLLRVGTVDFDTAGSEGDGFRFEGVADPLGVVQSVDRAQREF
                     AEHQIEQSAGYGGEPGTSVRRPPPADPGP"
     gene            complement(9242..10156)
                     /gene="gluQRS"
                     /locus_tag="VHF88_10220"
     CDS             complement(9242..10156)
                     /gene="gluQRS"
                     /locus_tag="VHF88_10220"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX2162180.1"
                     /translation="MPSGRFAPSPSGPLHLGNLRTALLAWLFARSDDEPFLLRIEDLD
                     PQRSRPEHERGQLADLRAIGVDWDGEPVRQSERTDLYRDALRRLERDGHVYPCWCTRA
                     EIRPAAEAPHGPGPEGAYPGTCRRLTAQQRAERERIAGRPPSLRLDVAVAPADRLAFD
                     DRIHGRVQGAIDDFVLWRWDDTPAYNLAVVIDDADQGVGEVVRGDDLIDSTPRQILLQ
                     RMLGLPTPAYAHVPLVIGADGARLAKRHGAVTLADRAALGDTPADVVAWMAASLRLAE
                     SGERPTVQELLMRFDPTVLPREPATFDV"
     gene            complement(10232..11182)
                     /locus_tag="VHF88_10225"
     CDS             complement(10232..11182)
                     /locus_tag="VHF88_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX2162181.1"
                     /translation="MPTEPTYPIRPLLIAALICLCCALAPASLPVADAVAQGPTATAA
                     GKSLKKGSRGKRVRQLQRALGIPADGIFGKGTRRAVKRYQRRRGLVVDGIAGPQTLAS
                     LGVGSRSASRGKARTRRVSSRGGGVRELQRALGVPADGAFGPQTQRAVKRYQRRHGLV
                     ADGVVGPQTRAALGIGPGKVLKRKGSGGSGSPGGSGRKARIIRAIIRAGNRIATHPYE
                     YGGGHGSWVDDGYDCSGSISYALHGGGLLRSPLDSSGFMSYGRPGPGRHITIYANAGH
                     AYMVIKGRRFDTSARSETGSRWSNEMRSSAGYVARHPPGF"
     gene            complement(11254..12270)
                     /locus_tag="VHF88_10230"
     CDS             complement(11254..12270)
                     /locus_tag="VHF88_10230"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162182.1"
                     /translation="MDQAVVARAPLRIPLGGGGTDLPSHYREHGGFAVSATIDRHVHV
                     MLSTALARRFRLKHLEWEDVERPEEIRHPILHAAVEHHWDGTPFELASVGDVPPGTGL
                     GSSSAYTVGVLRALATARGEQLSPEALAARACRLEIEQLSQSVGVRDQYAIAVGGVTA
                     VTLAPGGPGSEGGAWGDDITVRRLEVSDATLEELRDRFLLFFAGERQKTSQSMLSPLI
                     EGGDDAAMRRNLLRTAELARNAAAALETGDVASVGGLMDEQWALKLERTPSAITERIA
                     ELHSVALGRGRAGGAVLGGAGGGGFLLVYSEDPEATRAAMEATGAPELRFAIEQRGCS
                     AGPA"
     gene            complement(12328..12747)
                     /locus_tag="VHF88_10235"
     CDS             complement(12328..12747)
                     /locus_tag="VHF88_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162183.1"
                     /translation="MNRSIAATTVALALLAVPAGAVAKPAQADRSNATQECRAERGGS
                     DATREAFKVKYGTNENGRNAFGKCVSAKARAEARERKAARRKAAKQCRAEREEIGETA
                     FAEKYGTNKSKRNAFGKCVSQTAKTQHEQEQQPAPTS"
     gene            12953..>13720
                     /locus_tag="VHF88_10240"
     CDS             12953..>13720
                     /locus_tag="VHF88_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2162184.1"
                     /translation="MELVRAGDVAAFEVLYERHVAGVLGFCRHMLGSQVEAEDAVQQA
                     FVSAHDSMRSGDREIAFKPWLYTIARNRCLSILRARREQATELPELSTVGLNEQVERR
                     ADLRELVRDVQRLPEHQRAALVLSELGDLSHAEVAEVIGEQPHAVKGLVFRARAALTE
                     RRDARAADCAEIRVELAAATGGGLRRGRLRHHLDSCPDCTAYLDEVRRQRRLLGLALP
                     VAVAPALKDAVMSSAGIGGGAVGVASVGGAAAGGGAGV"
BASE COUNT         1826 a         4913 c         4876 g         2105 t
ORIGIN      
        1 tcgtcgacga gctctacgag atcgcgcatt cgcgtgcgct ctcggtggcg ccggtctacg
       61 gcggcgtcgg catccaggcc caggcgcgca aggccgccaa ggcgcacatc gtcgtcgcca
      121 cgccgggccg gctcgaggat ctgatccagc gccgcgagct gtcgctcgag cacgtccagg
      181 tgctggtcct cgacgaggcc gaccggatgc tcgacatggg cttcaagccc gccgtcgacc
      241 ggatcgtcgg ccagaccccc gacgatcgcc agacgatgtt cttctcggcg acgcttgagg
      301 gcgggaccgg ccaggtcgcc cagtcctaca cccgcgacgc gcgccgccac gtccacacgc
      361 cgaaggtcga gcgcaaggcc gacgtccagc accgcttcct ccacgtcgcc catgccgaca
      421 aggtcgacac gctgatccgc gagctgaacg agaccgagcg cggtcgcacg ctggtcttcg
      481 tgcgcaccaa gcgcggcgcg gaccggctcg tccagcggct cggcaagagc ggcatcaagg
      541 cggtcgcgat gcacggcaac aagtcgcaga accagcgcca gcgcgcgctc gcgaacttcg
      601 agtcgggcaa ggtcgacacg ctcgtcgcca ccgacgtcgc ggcgcgcggc atcgacgtcc
      661 ccgacgtcac ccacgtcatc aactacgacg ctccggagga tcgcgacacc tacgtccacc
      721 gcgtcggtcg caccggccgc gccggcgcga ccggtgccgg cgtcagcttc gtgatcggcg
      781 atcaggtgcg cgacatgcgc cggatcgccc aggacctcgg cctgctgagc gagttcgacc
      841 acgaggcgcc ggcccacgtc accggcccgc cgcgtgagat ggcggcgcgc ggcaacggcg
      901 gccatcgcgg ccagggccag aagcgcggcg gcggcaaggg ccgcggtcgc tccaacgacg
      961 gccagggccg cggccgcgtc agcgagtcgc gcgacggcac gggcggcaac cgccccaagc
     1021 cgcgtccgaa cagcgccggc acggcacgtg gcaacgtcgc gtcgtcgcgc ccgccgcgca
     1081 agcgccgctc gcgcgtctag gggcgcggtt aggctctcgg cgtgaccgag agccgagagc
     1141 gcctggatcg ctcgaccgtc gtggcgctcg ccgcgatggc gatggcggtc ttcgtgatcg
     1201 ccaacgacgt gacggcgctg agcgtcgcgc tgccggagat cgagtgcgac ctcgacgcga
     1261 gcgtcggcac ggtccagtgg gtgatcagcg cctacgcgct gatcttcggc gtgctgatcg
     1321 tcaccggcgg ccggctcgcc gacctgctcg gacggcggcg ggtcttcttc gttggggctg
     1381 cgattttcgc ggtcttctcg gtgctcggcg ccgtcgcccc gagcgcgctc acgctgatcc
     1441 tcgcgcgcgg cctgatgggc atcggcggcg cgctgatgtg gccggcgatc ctcggcatga
     1501 cgtactcgat cctgccggag aagcgcgccg cgctcgccgg ctcgctgatc atcggcgccg
     1561 ccggcttcgg caacgccgct gggccactga tcggcggctt cctgaccgac gcgctcgact
     1621 ggcgctggat cctcgcgctg aacctgccga tcgccgccgt cgccagcctc gtgacctggc
     1681 gttacgtgcc cgagtccagc ggcgacgacg ccgagcggcg cttcgactac gccggcgtcg
     1741 cgacgctctc ggtcggcctg atcgcgctgc tcgtcgcgct cgacgaggtc accgatctcg
     1801 gctggaccga cccgctcgtc ctcggcctgt tcgcgctctg cgtcgtcctg ctgatcgcct
     1861 tcgcggcgag cgagcgccgt gccggcagct gggcgctggt gcccgacgac gtgatccgca
     1921 accgtgcttt ccgctcggcc tgcctggccg tgctgctgat gtcgccgacg ttcttcgtcg
     1981 cgctgatgta cctgccgcag ttcatgcaga aggtgctcgc ctacacaccg gtcgaggcgg
     2041 ggctcgggct gctgccgatg atgggcgtct tcgcggtcgt gtcgttcgcc gccggtccgc
     2101 tctacgagcg tttcgggccg cgcccggtga tcgccgccgg tgcctcctgc atggtcgccg
     2161 gtccgctgct gatctcgctg atcgagcgcg gcgacagctg ggcccagctg gttcccggga
     2221 tggcggtcct ggggctcgga gtcggcctct tctacagctc gataactacc gccgcggtga
     2281 ccgcgctcga ttcctcgcgt tctagcctcg ccggcgggat cggctacatg ttccaggtcg
     2341 ccggcggttc aattgggctc ggcctggcga cgacggtctt caccgaggcc gtcgagcgcg
     2401 gcggcttcga ggacggcttc cagtgggcct tccgcctcgt cgctgcgctc gcgctcgtcg
     2461 cgctggccgt cgtgctgctc tacgtcgggc gcgccgagga ctacgaggca gacgacgact
     2521 cgtcggccga gctggcctcc ggcagcagcg cgtagagccg cgtacgaccg tcgatcccct
     2581 tgaggttgcg ccgccgcgag cgcttccagc cgtagccgcc gccggccagc tcgacgaccg
     2641 gctcggtggc gtagatccgg ttcggcttgc cggcgtcggt gacgcggctg gcgaggttga
     2701 ccgtggatcc gaaccagtcg ccgccgcgcg gtgtcgccgg cccgaacgcg acgccgacgc
     2761 gcagcggcgg gaactcctcg ccctccgcct cggtcgcggc gaccagcgag cgcagcgtct
     2821 cgagcatcgg gtcgacctcc caggagacca gcatcaccgc gtcgccgatc gtcttgacga
     2881 gctgtgtcgg gcggcagatc gagtcggtgg ccagctccgc cagccgcgtt gcgacctggc
     2941 ccagccgctc ggccggaagc tgctcgccga gcttggtgta gccgacgagg tcggcgaagg
     3001 cgatcgcgat gtcgcgcgtg ccggcgagcc ggccggcgtc gcgctcggcc tccgcgacta
     3061 ggtcgctgcg gatcccctcg cgcagctgcg tgcggaacgc cgagtcgagc acgaacgcga
     3121 cttccggcgc cagcgcctgc gccgcctcga catagcgcag cgccagcgtc aggtcggtgt
     3181 cgccgggccg gacgagcggg ctcaccgtcg tgcggcggat cgcctccgcg gtctgggaca
     3241 tcgcctggcc gatcacgcgc gacgtctcga gcaggccctc gcgcgggatg ccggcgtcgg
     3301 cgaacagctt gatccgccgc gccagctcga cgtcgtcgtc ggtgtaggcg cgctcacgcg
     3361 ggagcgccgc cgcccgtccg ctcgcctgca tcagccggcg caggaaggcg gggctgagcc
     3421 cggagagctt cgcgacgccg gtcagcgtgt gccggcggtc gccgcccagc gcgatctcga
     3481 ccgcgaacgt cgccaggcgg tcctcctgct ccgccgcggc gatcgcctgc tcgctctggc
     3541 cgagcgcgcg cagccgctcg atcagcgccg cgcgtgcccg ctcctgtgtc tcgtcgtcgc
     3601 tgggcgccgc cacggccccg agtgtcccag ccgcggcggc ggcgacgcgc gtctcaggag
     3661 atcttcatgc cagaaatcgc acgcgagatc accagccgct ggatctccga ggtgccctcg
     3721 aagatcgtgt agatcttggc gtcgcggtgc atccgctcga ccgggtactc gcgcgtgtag
     3781 ccgttgccgc cgaggatctg gatcgcccgc tcggtcgacc agaccgcgac ctcgccggcc
     3841 ttcagcttcg acatcgagcc ctcggcgttc tcgaacacca tccccgactg cttgcccatc
     3901 cacgaggcgc gccagacgag cagccgcgcg gcgtcgatct ccatcttcat gtcggcaagc
     3961 gtgaaggcga tcgcctggtt ctcgacgatc ttcttgccga actgctcgcg ctccttggcg
     4021 tagtcgagcg cgtactcgta ggcggcgcga gcgatcccga ccgcctgggc gccgaccgtc
     4081 ggacggcttg cctcgaaggt ctgcatcgcg gcctgggagc gcgcctgctt cccctcgcgc
     4141 acgcgcgcca gccgctcgtc gagcttctcc ttgccgccca gcaggcagga gcccgggacg
     4201 cggcagtcgt cgaggtgcac gtcggcggtg tgcgacgcgc gcaggccgtg cttcttgacc
     4261 ttggcgccct gctcgcagcc cttggtgccg ggcgggatga cgaacgccgc gtggccgcgc
     4321 gagccgagct cgcggtcgac cgaggcgatc acgacatgga ccttggcgat gccgccgttg
     4381 gtcgcccagg ccttctggcc gttgatcacc cactcgtcgc tcgcctcgtc gtacttggcg
     4441 gtcgtgcgga tcttcgagac gtcggagccg gcgtcgggct cggaggcaca gaacgcggcg
     4501 acggccggct cgtcctgact gccgaagcac tgggggatcc actcgccgat ctgctcgttg
     4561 ctgccctggc cgaagatcgc cgcgacggcc agtgaggtgc ccatgatcga catgccgatg
     4621 ccggcgtcgc cccagaacag ctcctcgttg acgatcggca tctgcaggcc ggtcgggtcc
     4681 atgaagaact gcgcgagcga ctcgaagccg tagaggccga tcttcgcggc ctcctggatc
     4741 accggccagg gagtctcctc gcgctcgtcc cactccgagg ccgcggggcg gacgacgttc
     4801 tcggcgaagc cgtggaccca gtcgcggatg tccttctggt cctggctcag ctcgagcgag
     4861 aacggcgtct cgccggcctc ggggcctgtg ccgtcgccgg tcagctcggc gacgctgtcg
     4921 gtggcgctca tctgtggggg cagctcctgg ctagatatga gggtgttcct gggcgtcatg
     4981 ctacggggtg gcccatttgg ggggtgcagg ggcgggtgcc gttatcgccc tgttgacgtg
     5041 ggtagaggat cggcatggct gagaacgagg gacaacagaa gacgccggac gtgcagcgcg
     5101 cgagctcgac gagcccgctc gacgggagga ccgacaaggc gaccaagccg cccggtcagg
     5161 gcgagatcga tcgcgaggcg gtcgacaagg gctgggaggc gctcgacagg gccggcgggg
     5221 gccactgacc cgccgcgtcc cggcccgacg ctctaggggc ccgtccatga gtaactaggc
     5281 ggcgcaggta gggtctgcgc cgatgaccca tgccgatgcg ccgcaggtcc tctggcagcc
     5341 gagcgaggag ctgaagcagg gctcggcgct cgcggcctac atcgagtggc tgaaggtcga
     5401 tcgcggcgtg gcgatcgaga cctacgacga gctctggcgc tggtcggtcg acgacctcga
     5461 cgggttctgg gcctcgatct gggactactt cgacgtgcgg gcgtcggctc cgtacgacgc
     5521 ggtgctcggc agtcgcgcga tgccgggggc ggagtggttt cccggcgcgc agctgtccta
     5581 cgccgagcac gtcttccgcg accgcgagga ttccgcggtg gcgattcgcc acgcctccga
     5641 gctgcgcggc ctctccgagc tcagctgggg tcagctgcgc tcgctgaccg cgcggatcgc
     5701 cgggggactg cgcgacctgg gcgtcggtcc cggcgaccgc gtcgtcgcct acctgccgaa
     5761 catccccgaa acgatcgcgg ccttcttcgc ctgcgcgtcg atcggagccg tctggtcgag
     5821 ctgctcgccg gacttcggcg tgcgctccgt ggtcgaccgc ttcgcgcaga tcgagccgag
     5881 gctgctgttc gcggtcgacg gctaccgcta caacggacgc gacttcgacc ggatcgacac
     5941 gatcgcggca ctgcagcgcg agattccgtc gctcgagcac accgtgctgc tgccgtacct
     6001 cgacagcgcg gcggatgcgg gacggctggc cggcggaatc acgttcccgg agctgctcga
     6061 gcgcggcgaa gacacgccgc tcgagttcgc ccagctgccg ttcgaccacc cgctctgggt
     6121 cctctactcc tcggggacga ccggtctgcc gaaggcgatc gtccacagcc agggcggcat
     6181 cctgctcgag cacctcaaga agctcaacct gcacctcgac gcgcgacccg gcgaccgggt
     6241 cttctggttc accacgaccg gctggatgat gtggaacttc ctcgcgggag ttttgctgac
     6301 gccagcgtcg atcgtcctct acgacggcag ccccgcccat ccggacctcg gcgtgctgtg
     6361 ggatctggcc gagcgcgcgg gcgtgacctg cttcgggacc tcggctgcct acatcgcctc
     6421 gtgcatgaag ggcggcgtcg agccggggga ggggcgcgac ctgggtgcgc tgcgcagcgt
     6481 cggctcgacc ggctcgccgc tgtcgcccga gggctttggg tgggtctacg accacgtcgg
     6541 ctccgacacc tggctgttct cgacctccgg cgggaccgac gtctgcaccg cgttcgtggg
     6601 cggctgcgtg ctgctgccgg tctacgtggg agagctgcag ggccgctcgc tcggagccga
     6661 tgtccaggcc ttcgacgagg acggcaatgc cgtggtcggg gaggtcggcg agctggtgat
     6721 ctcccagccg atgccttcga tgccgatctt cttctggaac gaccccggcg gcgagcggct
     6781 gcgcgagagc tacttctcga tgtacccggg cgtctggcgc cacggcgact ggatcgagat
     6841 caccgagcgc gggaccgcga tcatctacgg ccgctccgac tcgacgatca accgcggcgg
     6901 gatccggatg gggaccagcg agatctaccg ggcggtgctc gcagtcgacg agatcgtcga
     6961 cgcgctcgta gtcgacgtgc ccgcgagcca gtccgccgcc gcccgcgccg cgggcgtcgc
     7021 cgaggacgac agctggatgc cgctgttcat cgtgctgcgc gacggggcga cgctcgacga
     7081 cgatctcgag cgcgagatcg cgcgccgtgt gcgcgccgag tgctcgccgc gtcatgtgcc
     7141 cagcggcgtc tacgcgatcg ccgaggtgcc gcgcacgctc tccggcaagg tgctcgaggt
     7201 cccggtcaaa cgaatcctga tgggcacgcc gcccgagcag gccgctgcgc gcgactcgct
     7261 ccagaacccc gccgcgctcg actggttcgt cgagctcgcg tcgggtcacg acggcgccta
     7321 gccgcgtccg ggcgtcagtc gccggccgcg gcgagccgct cagcgaggcg cttcatcccg
     7381 agctcggccg cagccgcggc gccgccggcg cggtcggtcg gcgcgtccgg gggtcgctcg
     7441 acgtcgacgg gccgcagcgt ggcgatctcg cggaaggcgc gcagctggtc ggcctggcgg
     7501 cagagcgagt tggcgtcggg gccgtcacgg tcggcctcgg cgagcgcggc ctcgagcgag
     7561 ccgtggcgac gcaggatgtc ggccgcgcgc ttctcgccga tccccttcgc gcccggtagg
     7621 ccgtcggaag ggtcgccgcg cagcgcaatg aagtcgggga cctgctcggg ctcgacgccg
     7681 tagcgctcgc gcacctgcgg cggaccgatc tcctccgggc ccttcttgcc gcgcccgccg
     7741 agcagcagca cgcgcacgga gtccgtcgcg cactggaaca tgtcgcggtc gccggtcagc
     7801 accagggcct cgccgccggc ctgctgctcg acgcgggcgt aggcgccgag caggtcgtcg
     7861 gcctcgagct cgtcggtgtc ggtgatctgc cagccgaggg cgccatacag gcggggcgcc
     7921 tgcgcccact ggtgcgcaag gtcgtccggc accggcggcc ggtgggcgtg atagccggga
     7981 tataggttga cgcggtaggg ggccgcgtcg ggcccgaagc agcagacgac ggcccgcgcg
     8041 ccgcgctcct cggcctgcgc gaggatcgtg ttgacgctcc ccagcagcgc attgaccggc
     8101 cgtccgtcgg cccctcggat cttgtccggg agcccgaaga aggcccggta gaggaggtag
     8161 ggagcatcca cgagtagcaa tgggtcggcc atcggtgcta ctttgacggg tcgtaaggcc
     8221 ggaaagtccg gcgaaagtag tgccagcgct cgcagtcgtt gctcatcagc atcctccgcg
     8281 tgtacgcagc acagagaaag atcgtggagg aattccatgc caggcggaac cgtgaagtgg
     8341 ttcagcgacg agaagggctt cggcttcatc acccccgacg acgggggcaa ggatctcttc
     8401 gtgcaccaca caggcatcag cggtgacggc ttcaagtcgc tcaccgaggg cgcccgcgtg
     8461 agctacgacg cggagcaggg tgacaagggc cccaaggccg taaacgtcat ccagtcctaa
     8521 gcgctcgcat tacgcgaaag cttcagaaag ggccccgctt gcggggccct ttctcgttct
     8581 cggggtcgct tgcgtgagct aggctctcgg cgatgccgtc gatccagcta gcgcaggacg
     8641 agcacatcgt cttcgagggc catccctcat ggcgtgcgac gctggccttc tacatcaagg
     8701 gcatcctcgg tgcgatcgtg ctgctcgccg cgttcgcggg gatcgccagc ctcttcgccg
     8761 acggcgtcga cacgacgacg ctcgcaatcg tcgggctcgt cctgctcgtc ctcgtgctcg
     8821 tcggcggcta cgtctggcgg atggcgacga cctacacgat caccaaccgg cgcctgcaca
     8881 tcaagcgcgg gatcgtggcg cgcaagacgc aggaggcacg cctcgagcgc gtccagaacg
     8941 tcaacaccga ccagtcggtg ctcggccgct tgctgcgcgt cggcaccgtc gacttcgaca
     9001 ccgccggaag cgagggcgac ggcttccgct tcgagggtgt cgccgacccg ctcggagtcg
     9061 tccaatcggt cgaccgcgcc cagcgcgagt tcgccgagca ccagatcgag cagtccgccg
     9121 gctacggcgg cgagccgggg acctcggtgc ggcggccgcc cccggccgac ccagggccct
     9181 aactgggcgt acatcggcgg tagtaccgcc gatatcggcc cagctcgaag tggcagacgt
     9241 ctcagacgtc gaacgtcgcg ggctcgcgag gcaggacggt tgggtcgaag cgcatgagca
     9301 gctcctgaac agtcgggcgc tcgccggact ctgcgagacg gaggctcgcg gccatccacg
     9361 cgacgacgtc cgccggcgtg tccccgagag ccgcgcggtc ggcgagcgtg acggcgccgt
     9421 ggcgcttggc gaggcgcgca ccgtcggcgc cgatcacgag cggcacgtgg gcatacgcgg
     9481 gcgtggggag gccgagcatc cgctgcagca ggatctggcg cggcgtcgag tcgatcaggt
     9541 cgtcgccgcg gacgacctcg ccgacgccct ggtcggcgtc gtcgatgacg accgcgaggt
     9601 tgtacgccgg cgtgtcgtcc cagcgccaga gaacgaagtc gtcgatcgcg ccctggacac
     9661 gtccatggat gcggtcgtcg aaggccaggc gatcggcagg cgcgaccgct acgtcgagcc
     9721 gcaggctcgg cgggcgcccg gcgatgcgtt cacgctcggc gcgctgctgg gcggtcagcc
     9781 gccggcaggt gccggggtat gcgccctcgg gtccaggccc atgtggggcc tctgcggcgg
     9841 ggcggatctc ggcgcgcgtg caccagcagg gatacacatg accgtcccgc tcgagtcgcc
     9901 gcagggcgtc ccggtagaga tccgtgcgct cggactgccg taccggctcg ccgtcccagt
     9961 cgaccccgat cgccctcaaa tccgcgagct ggcctcgctc gtgctcgggc cgtgaccgct
    10021 gcgggtcgag gtcctcgatc cgcagcagga acggctcgtc gtccgagcgc gcgaacagcc
    10081 acgccagcag cgccgtgcgc aggttcccga gatgcagcgg gccgcttggg ctgggtgcga
    10141 agcggccgga cggcaccgtg cgagcttagg cgctcgtcgt ggctctcctc cgaagcatcc
    10201 ttgggcgtga gccctaaaga tgcttcgagc gctagaagcc cggcgggtgc cgggcgacgt
    10261 aaccggccga cgagcgcatc tcgttcgacc agcgcgagcc ggtctccgag cgagcgctgg
    10321 tgtcgaagcg ccggcccttg atgaccatgt acgcgtggcc ggcgttcgcg tagatcgtga
    10381 tgtggcggcc gggcccgggc cgcccgtagc tcatgaagcc gctcgagtcg agcgggctgc
    10441 gcagcaggcc gccgccgtgc agcgcgtagg agatcgatcc cgagcagtcg tacccgtcgt
    10501 cgacccacga cccgtggcca ccgccgtact cgtaggggtg ggtcgcaatc cggttgccag
    10561 cgcggatgat cgcccggatg atgcgcgcct tgcggccgct gccacctggg ctaccgcttc
    10621 cgccgctgcc cttgcgcttg agcaccttgc cgggcccgat tccgagcgcc gcccgcgttt
    10681 gcggaccgac gacgccgtcg gcgaccaggc cgtggcgacg ctggtagcgc ttgaccgcgc
    10741 gctgggtctg gggaccgaat gcgccgtcgg ccgggacgcc gagcgcgcgc tgaagctcgc
    10801 gcacgccgcc gccgcgcgag ctgacccgac gcgtacgcgc cttgccgcga ctggcggagc
    10861 gcgacccgac gcccaggctt gccagcgtct gggggcccgc gatgccgtcg acgaccaggc
    10921 cgcgccgacg ctggtagcgc ttgaccgccc gccgcgtgcc cttgccgaag atcccgtccg
    10981 cggggatgcc gagcgcgcgc tggagctggc gcacccgctt gccccgggaa cccttcttga
    11041 gcgacttgcc cgccgcggtg gcggtcggtc cctgcgccac ggcatcggcc acgggcaggc
    11101 tggcaggcgc cagcgcgcag cagaggcaga tcagcgcggc gattagcagt ggacgaatcg
    11161 gatatgtggg ctccgtaggc acgaagcgcg tcgttgtatc aggcaaagct cctgcaagtt
    11221 gcaggatttc ttgcatgcaa cgggtcttgc agctcaggcc gggccggcgc tgcagccgcg
    11281 ctgctcgatc gcgaagcgca actcgggcgc gcctgtcgcc tccatagcgg cgcgcgtggc
    11341 ctccgggtcc tcgctgtaga cgagcaggaa gccgccgcca ccagctccgc cgaggaccgc
    11401 cccgccggca cgcccccggc cgagcgccac ggagtgcagc tctgcgatcc gctcggtgat
    11461 ggcagacggc gtgcgctcga gtttgagtgc ccactgctcg tccatcaggc cgccaacgct
    11521 ggcgacgtcg ccggtttcca gcgcggccgc cgcgttgcgc gcgagctccg ccgtccgcag
    11581 caggttgcgt ctcatggcgg cgtcgtcacc tccctcgatc agcggcgaga gcatcgactg
    11641 cgacgtcttc tgccgctcgc cggcgaagaa cagcagaaag cgatcgcgca gctcctcgag
    11701 cgtcgcgtcg gagacctcga ggcgtcgcac cgtaatgtcg tcgccccagg cgccgccttc
    11761 cgagcctggt cccccgggcg ccagcgtcac cgccgtgacc ccgccaacgg caatcgcgta
    11821 ctggtcgcgc acgccgacgc tctggctgag ctgctcgatc tcgagccggc aggcccgcgc
    11881 cgcgagcgcc tccgggctca gctgctcacc gcgcgcggtc gccagcgctc gcagcacgcc
    11941 gacggtgtag gcgctcgacg agccgagccc cgttccaggc gggacgtcgc cgaccgacgc
    12001 cagctcaaag ggggtcccgt cccagtggtg ctcgacggcc gcgtggagga tcggatgacg
    12061 gatctcctcg ggtcgctcga cgtcctccca ctcgagatgc ttgagccgga accgccgtgc
    12121 cagcgcggtc gacagcatca cgtgcacgtg gcggtcgatc gtcgccgaca cggcgaaccc
    12181 cccgtgctcg cggtagtgcg agggaaggtc ggtgccgccg ccgcccaggg ggatgcgcag
    12241 cggcgcgcgc gctactacgg cctgatccac gcgcgcatga tcgagcatcg gcgcggccct
    12301 cgtcgggccg cgcctgagct cggtcgccta tgaggtcgga gccggctgct gctcctgctc
    12361 gtgctgcgtc ttcgcggtct gcgagacgca cttgccgaac gcattgcgct tgctcttgtt
    12421 ggtgccgtac ttctcggcga acgccgtctc gccgatctcc tcacgctctg cgcggcactg
    12481 cttagcagcc ttgcgccgcg cggccttgcg ctcgcgtgcc tcggctcggg ccttcgcgga
    12541 cacgcacttg ccgaacgcgt tgcgaccgtt ctcgttcgtg ccgtacttca ccttgaacgc
    12601 ctcgcgcgtg gcgtccgagc cgccacgctc tgcgcggcac tcctgggtgg cgttgctgcg
    12661 gtcggcctgc gccggcttgg cgaccgcgcc ggccggtacg gcgagcagcg cgagcgccac
    12721 tgtcgttgct gcgatcgatc tgttcatgga gaccttcctc gttggggact cacgactatc
    12781 aacgcatcgc cgacgtgcag gttcggcagt aggtactcgc gaacccctgg cccccgaccg
    12841 cgttccgtag gacaatgacc gcgtggagcg catcgcccga gcccctgcgc aggtacgtgc
    12901 tccggtccgt ggaccgaagc gtctgctcgc cctgcgctct gacgcgcgcc tggtggagct
    12961 cgtgcgcgcg ggcgacgtcg ccgccttcga ggtcctctac gagcgtcacg tcgcgggcgt
    13021 gctcggcttc tgccgtcaca tgctcggctc gcaggtggag gccgaggacg ccgtccagca
    13081 ggcgttcgtc tcggctcacg actcgatgcg cagcggcgat cgcgagatcg ccttcaagcc
    13141 gtggctctac acgatcgcgc gcaaccgctg cctctcgatc ctgcgcgcgc gacgcgagca
    13201 ggccaccgag ctgccggagc tctcgaccgt cggcctcaac gagcaggtcg agcgccgcgc
    13261 cgacctgcgc gagctggtcc gcgacgtcca gcggctgccg gagcatcagc gcgcggccct
    13321 cgtgctcagc gagctcggcg acctctccca cgccgaggtc gccgaggtga tcggcgagca
    13381 gccgcatgcg gtcaagggac tcgtcttccg cgcccgcgcc gccctgaccg agcggcgtga
    13441 cgcacgagcg gccgactgtg ccgagatccg cgtcgagctg gcggccgcga ccggtggcgg
    13501 gttgcgccgc ggccggctgc gtcaccacct cgacagctgc ccagactgca cggcctacct
    13561 cgacgaggtg cgccgccagc gtcggctgct cggcctcgcc ctgccggtgg ctgtggcgcc
    13621 tgcgctgaag gacgcggtga tgagcagcgc cggcatcggg ggcggcgctg tcggagtggc
    13681 cagcgtcggc ggtgccgccg cgggcggcgg cgccggtgtc
//
LOCUS       DATABV010000220         9508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2723979, whole genome shotgun sequence.
ACCESSION   DATABV010000220 DATABV010000000
VERSION     DATABV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723979"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VHF88_10245"
     CDS             <1..375
                     /locus_tag="VHF88_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162185.1"
                     /translation="VPGGDLVDRRGATWAVEGELAVLGLSAVGGRLEEGDYPDPLTRL
                     WSALACRRAGDVLLSATPGYEFVDWGGSHHENGGSHGSLHRCDSLGVLLTCGVAAGAP
                     RPAQPHLRDVTPMIAGHFGLIS"
     gene            377..886
                     /locus_tag="VHF88_10250"
     CDS             377..886
                     /locus_tag="VHF88_10250"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX2162186.1"
                     /translation="MGDQPDSPAARGLEPPAAATAPAPPLAPGARLRAGVRKPQNWVQ
                     LAKFCMVGGSGYVVNLAVFSLAVAVGDLHHLVAATLAFLVAVTNNFWWNRNWTFAARD
                     GRAGFQAARFLVVSVAAFVFAATVLELLVTGAGIAEIPAQAISIVVATPLSFLGNKIW
                     SFGSGDWRA"
     gene            892..2646
                     /locus_tag="VHF88_10255"
     CDS             892..2646
                     /locus_tag="VHF88_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162187.1"
                     /translation="MALLARAALLVAAVLLALALAGVSAGAASAAPELKTPESQTVPP
                     EGYELTAKRAIRIAGRSEKIRAERARHDEFRAAAYTRGPGQWQVSWFEGDREVAQARV
                     DDSLGVVLEAWTGHQVAWSMARGYDGAFGRKLNAPYVWIPLCLLFLLPFVDPRRPFRL
                     LHLDLLVLLAFGASHYFFNRGEIGVSVPLVYPVLLYLLARLLWHGLRPRERPQRLIPL
                     IPLSWLAFGLIFLVTFRVGLNITDSNVIDVGYAGVIGADRIADGEGIYGPGFAPDVEH
                     GDTYGPLTYLSYVPFEQVLPWSGTWDDLPAAHGASIAFDLLTLLGLLILGRRLRAGPA
                     GDELGVALGYAWLTYPYAIFALQTNSNDTLVGLLTVAALLALTLAPARRGLSAAARGG
                     AVALAAAAKVGPLALAPLFATADTGPTRRSTLRLLLIFSAALAALLAVCFLPFMPDGG
                     LRELYDRTLGYQASRRSPFSVWGQIDDGETYRSAMTGLALLLAVAVAFVPRRKSPLQV
                     AALGAAVLVAVQLAASHWFYLYVVWFFPLAIVALFGVYAGAPDRRSDAGRLEDLDDAG
                     GAPVAVGLDQHAVQPRVL"
     gene            complement(2551..3330)
                     /locus_tag="VHF88_10260"
     CDS             complement(2551..3330)
                     /locus_tag="VHF88_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX2162188.1"
                     /translation="MKLLFVGDVVGKLGRQTLAALLPGLRERHQPDFVVVNGENAAGG
                     VGITQRTARDLFETGADAITLGNHAYRHSEVYELLDNEPRIVRPANYPKGSPGRGLTV
                     IENGALRLGVVNLSGSLFVDAARSAFPEVDSALADLRGRADHVLVDMHAEATSEKVAM
                     GWHVDGRVTACLGTHTHIPTADARVLPGGTAYCTDVGMTGPRGGVIGVKRELALKRFL
                     TMTQVRFETSSEDPWLNGVLVEADGDGRATGIVQILEPASI"
     gene            3358..4101
                     /locus_tag="VHF88_10265"
     CDS             3358..4101
                     /locus_tag="VHF88_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2162189.1"
                     /translation="MADGGAVVASAGQGALVAEGIVKRFGAREALRGVSLSASPGELV
                     ALVGPNGAGKTTLLSILAGIQRADAGSVSRAAREIGWVPQQAALYGKLTVAENLRLFA
                     RLERIADAEAAVAEMLALTGLADRADDLAGELSGGNRQRVNIAIGLLGRQEILLLDEP
                     SAALDPRQRERVWEFVLGLARRGTAVVYSTHDLSEAARHADRVVVLADGETLFEGSPT
                     ALGETVEREGGSHSDFEEAFVAFLRQRGH"
     gene            4103..5476
                     /locus_tag="VHF88_10270"
     CDS             4103..5476
                     /locus_tag="VHF88_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2162190.1"
                     /translation="MRWLLFKDLRILRRSPGLVALLVLYPIVLSVLIGFALSAGPDKP
                     KVAFVSLVPPERGALELGGEQLDLSRYEGRLFDAIDPVPVDCEGKSPLQCKDAAIDEV
                     ESGTAIAALVIPEDFTDRLSGVQAIALSGPPTIEVFYNADDPVKASYVEDMIKAELRD
                     ANLALTTQFTEIGLSYLDVVVSGGRVDVPVLEDLDILGLQRAEKIVERVRPEVSPERR
                     AELDEVIRFSRLARENLDLSGPLLRALREPIRVEQTALDGGSTSLSSFAVALAVAVSL
                     MFVTLLLAAGTLALEREENAFARLVRGLVSRTGLLVEKAGLAALCSLAICIAMLVGLG
                     LFVDLDWSRSPLWVAALAVAAIAFGAMGVAVGAVTREVRAASLLCVMLSLPLAFLALV
                     PSGSVAPAVYDATRAVSALFPFRPTLDALDAALNDSGGLVAPLLHLAALAAAFGLLAR
                     LALRRFA"
     gene            complement(5514..6077)
                     /locus_tag="VHF88_10275"
     CDS             complement(5514..6077)
                     /locus_tag="VHF88_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX2162191.1"
                     /translation="MAVREVLLYPHPALKATAAPAVVAPAGGAGHDMAVGDLAETERA
                     ARDLLETMVSFGHCVGLAATQLGDLTRIVAVDVTGHKKATVESGLLLLANPRVVEQDG
                     AEVAREGCLSIPHLTANVRRATSIVVEADALALTDDGLEVTQRRIETEGFEARCLLHE
                     IDHLDGILFLDRVDSLTRDVFERKNYQ"
     gene            complement(6087..6626)
                     /locus_tag="VHF88_10280"
     CDS             complement(6087..6626)
                     /locus_tag="VHF88_10280"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2162192.1"
                     /translation="MPQLALSAVGRDRPGIVAATTGVLLRHGVNVEDSRMSILRGHFA
                     MTLILGLPDGADRKALSADLDTVATDLGLEALALSAVEPLAEAVPAATHVVSIYGVDH
                     PGIIHHATRVLAEHRVNITDLETRLVEGDGSPSLYALLIEVAVPGDVDPADVDSALAE
                     IAASEVVEVSLRELESHEL"
     gene            6654..7628
                     /gene="hemB"
                     /locus_tag="VHF88_10285"
     CDS             6654..7628
                     /gene="hemB"
                     /locus_tag="VHF88_10285"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX2162193.1"
                     /translation="MPFPATRMRRLRGSGLLRDMVRETELTPAHLVQPLFVELGTDSR
                     TPIEAMPGVERLSISHAVEEAGEIKELGVPAVLLFGLPADKDDQGSGAYDDEGVVQLA
                     VRAIKEAHPELLVITDVCLCAYTAHGHCGIVRPDGAIDNDVTLELLAQTAISHARAGA
                     DAVAPSDMMDGRVAALRGQLDAEDLKETPIVAYSAKFASAFYGPFREAADSTPAFGDR
                     RTYQMDPANSDEAVREALLDMEEGADVLMVKPALPYLDVIQRVKEATAMPLAAYNVSG
                     EYAMIKAAAAAGHIDERATVLETLTSIRRAGADIVITYHAKDAARWLR"
     gene            7616..8704
                     /locus_tag="VHF88_10290"
     CDS             7616..8704
                     /locus_tag="VHF88_10290"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX2162194.1"
                     /translation="MASVREAAAIPKVRSRKDGAAVPLDELDKRLLNLMQGSFLIAPR
                     PYAHVAGLAEVSEDEVMRRVQRLLDERIIRQVTPIFDTRVLGYSSMLVAAKVDPENPH
                     RAAKIVNSHPGVSHNYLRNHDFNMWFTIATEPGSKLGLDGTLDVLAELTGAESVRQLP
                     TIKLFKIRMDLEMEKGTRDLAAAGIAQDPIEPDPIEPTELDVAVIRALQGDMPVIPEP
                     YAPAAAEVGISQEQLLDHLESMRERKALRRVAAILFHRRAGFSANGMGVWAVPEERIM
                     EVAPLMASFRGISHCYQRPTYDDWHYSVFTMAHGRSKEECDAILDSIAEESGIDDRRT
                     LYSSTEFKKIRLLYFTDDHRRWEAEHAG"
     gene            8745..>9508
                     /locus_tag="VHF88_10295"
     CDS             8745..>9508
                     /locus_tag="VHF88_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2162195.1"
                     /translation="MPGGVNSPVRAMRSIGRDPLFIAAGEGAEIVDVDGNRYVDYVSS
                     WGPLIAGHAHPRVIEAVGAAATRGTSFGAPTAAEVEIAAEIVARVPGAEMVRMVNSGT
                     EASMSAIRLARAATGREKLLKFAGAYHGHVDGLLADAGSGLATAGIPASPGVPAAQAA
                     ATVVVPWNDPTAVEQALAEHEVAAILAEPYPANMGLIPPADGFLAFLREQAGLGRDEA
                     HVGRIGLHEDRRELVLVEGGADRCLVVPGDDHGVRRR"
BASE COUNT         1205 a         3485 c         3349 g         1469 t
ORIGIN      
        1 gtccccggcg gcgacctcgt ggaccgccgt ggcgcgacct gggccgtcga gggcgagctc
       61 gcggtactag ggctgtcggc cgtcggcggt cggctcgagg aaggcgacta cccggatccg
      121 ctcacccgtc tgtggtcggc gcttgcctgc cggcgcgccg gcgacgtgct gctgtcggcg
      181 acgcccggct acgagttcgt cgactggggc gggtcgcatc acgagaacgg cggcagccac
      241 ggctcgctgc atcgctgcga ctcgctcggc gtgctgctga cctgcggcgt cgccgctggc
      301 gcgccgcggc ccgcccaacc ccatctgcgt gacgtcaccc cgatgatcgc cggccacttc
      361 ggtctaatct cgtagcgtgg gagatcagcc cgactcaccc gccgcgcgcg gtctcgagcc
      421 gcctgccgcc gcgaccgccc cggcgcctcc gctggcgccc ggcgcgcggc tgcgcgccgg
      481 cgttcgcaag ccccagaact gggtccagct ggccaagttc tgcatggtcg gcgggtccgg
      541 ctacgtggtc aacctcgccg tcttctcgct cgcggtcgcc gtgggcgacc tgcaccacct
      601 ggtcgccgcc acactcgcgt tcctggtcgc ggtcacgaac aacttctggt ggaaccgcaa
      661 ctggacgttc gccgcgcgcg acggtcgcgc cggcttccag gcggcgcgct tcctcgtcgt
      721 cagcgtggcg gcgttcgtgt tcgccgcgac cgtgctcgag ctgctggtca ccggcgccgg
      781 gatcgccgag atcccggccc aggcgatctc gatcgtcgtc gcgacgccgc tgagctttct
      841 cgggaacaag atttggagct tcgggtccgg ggactggcgg gcctgacgcg cgtggcgctc
      901 ctcgctcgcg cggcgctgct cgtcgctgca gtcctgctcg cgctggcgct cgcgggcgtc
      961 agcgccggcg cggcaagcgc ggcgccggag ctgaagacgc ccgagtcgca gaccgtgccc
     1021 ccggagggct acgagctgac ggcaaagcga gcgatccgga tcgcgggccg ctccgagaag
     1081 atccgcgccg agcgcgcgcg ccacgacgag ttccgtgccg cggcctacac gcgcggcccc
     1141 gggcagtggc aggtgagctg gttcgagggc gatcgcgagg tcgcgcaggc gcgcgtcgac
     1201 gactcgctcg gggtggtgct cgaggcctgg accggccacc aggtcgcctg gtcgatggcg
     1261 cgcggctacg acggggcctt cggccgcaag ctcaatgcgc cgtacgtctg gatcccgctc
     1321 tgcctgctgt tcctgctgcc gttcgtcgac ccgcgccgcc cgttccgcct gctccatctc
     1381 gacctgctgg tgctgctcgc cttcggcgcc tcgcactact tcttcaaccg cggcgagatc
     1441 ggggtctcgg tgccgctcgt ctatccggtg ctgctgtacc tgcttgctcg cctgctctgg
     1501 cacgggctcc gcccgcgcga acgcccgcag cggctgatcc cgctgatccc gctctcgtgg
     1561 ctggcgttcg ggctgatctt cctcgtcacc tttcgcgtcg ggctcaacat caccgactcg
     1621 aacgtgatcg acgtcggcta tgccggggtg atcggcgccg atcgcattgc cgacggtgag
     1681 ggcatctacg gtcccgggtt cgcgcccgac gtggagcacg gcgacacgta cggcccgctc
     1741 acctatctct cctacgtgcc gttcgagcag gtgctgccct ggagcgggac gtgggacgat
     1801 ctgcccgccg cccacggcgc gtcgatcgcc ttcgatctgc tgacgctgct cggcctgctg
     1861 atcctcggcc ggcgcctgcg tgccgggccg gccggagatg agctcggcgt cgcactcggc
     1921 tacgcctggc tgacttaccc gtacgcgatc ttcgcgctgc agaccaactc gaacgacacg
     1981 ctggtcggac tgctgaccgt tgcggcgctg ctggctctga cgctcgctcc agcgcgccgc
     2041 gggctgtccg cggcggcccg cggtggggcg gtcgcgctcg ccgccgccgc gaaggtcggc
     2101 ccgctcgcgc tggcgccgtt gttcgcgacg gcggacacgg gccccacgcg ccgttcgacg
     2161 ctgcgccttc tgctgatctt ctcggccgcg ctggcggccc tgctcgccgt gtgcttcctg
     2221 ccgttcatgc cggacggcgg cctgcgcgag ctctacgacc gcacgctcgg ctatcaggcg
     2281 tcgcgccgct cgccgttcag cgtctggggc cagatcgacg acggcgagac ctaccgctcg
     2341 gcgatgaccg ggctggcgct tctgctcgcg gtcgccgtcg ccttcgtccc gcggcgcaag
     2401 agcccgctcc aggtagccgc gctcggcgcg gcggtgctgg tcgccgtcca gctggcggcg
     2461 tcgcactggt tctacctgta cgtcgtctgg ttctttccgc tggcgatcgt cgcgctcttc
     2521 ggtgtctacg ccggcgcgcc ggaccgacgg tcagatgctg gccggctcga ggatctggac
     2581 gatgccggtg gcgcgcccgt cgccgtcggc ctcgaccagc acgccgttca gccacgggtc
     2641 ctctgacgac gtctcgaagc gcacctgggt catcgtcagg aagcgcttca gcgccagctc
     2701 gcgcttgacc ccgatcacgc cgccgcgcgg gccggtcatg ccgacgtcgg tgcagtaggc
     2761 ggtgccgccg ggaagcacgc gcgcgtcggc ggtcgggatg tgggtgtggg tgccgaggca
     2821 ggcagtcacg cgcccgtcga cgtgccagcc catcgcgacc ttctcgctcg tcgcctcggc
     2881 gtgcatgtcg accagcacgt ggtccgctcg cccgcgcaga tcggcgagcg cggagtcgac
     2941 ctccgggaac gccgaacggg cggcgtcgac gaacaggctg cccgacaggt tcacgacgcc
     3001 gagtcgcagc gcgccgttct cgatcaccgt gagcccgcgc ccgggactgc ccttcgggta
     3061 gttggccggt cgcacgatcc gcggctcgtt gtcgagcagc tcgtagacct cgctgtgacg
     3121 gtaggcgtgg ttgccgagcg tgatcgcgtc agcgcccgtc tcgaagaggt cgcgcgcggt
     3181 gcgctgggtg atgccgacgc cgccggcggc gttctcgccg ttgacgacga cgaagtcggg
     3241 ctggtggcgc tcgcgcaggc ccggcagcag cgcggcgagc gtctgccggc cgagcttgcc
     3301 gaccacgtcc ccgacgaaga gcagcttcat cgtgcgcagt ctgccaccct ccgcgccgtg
     3361 gccgatggag gagctgtcgt cgcgagcgcc ggacaggggg cgctcgtcgc ggaggggatc
     3421 gtcaagcgct tcggcgcgcg cgaggcgctg cgcggcgtct cgctgagcgc atcgcccggg
     3481 gagctggtcg ccctcgtcgg gcccaacggc gccggcaaga ccacgctgct gtcgatcctc
     3541 gccggcatcc agcgcgccga cgccggcagc gtcagtcgcg ccgcgcgcga gatcggctgg
     3601 gtgccgcagc aggcggccct ctacgggaag ctgaccgtcg ccgagaacct gcgcctgttc
     3661 gcccggctcg agcggatcgc ggacgccgag gcggcggtcg cggagatgct cgcgctgacc
     3721 ggcctggccg accgtgccga cgacctcgcc ggtgagctct cgggcggcaa ccggcagcgc
     3781 gtcaacatcg cgatcggcct gctcggccgc caggagatcc tgctgctcga cgagccgagc
     3841 gccgcgctcg acccgcgcca gcgcgagcgc gtctgggagt tcgtgctcgg cctcgcgcgg
     3901 cgcggcaccg ccgtcgtcta ttcgacccac gacctgagcg aggccgcgcg ccacgccgat
     3961 cgcgtcgtcg tgctggccga cggcgagacg ctcttcgagg ggtcgccaac cgcgctcggc
     4021 gagaccgttg agcgcgaggg cggcagtcac agcgacttcg aggaggcgtt cgtcgccttc
     4081 ctgcgtcagc ggggtcactg atatgcgctg gctgctcttc aaggacctgc gcatcctgcg
     4141 ccgctcgccg ggcctggtcg cgctgctggt cctctacccg atcgtcctgt cggtgctgat
     4201 cggcttcgcg ctctcggccg gccccgacaa gccgaaggtc gcgttcgtca gcctcgtccc
     4261 gccggagcgc ggcgcgctcg agctcggcgg cgagcagctc gacctgtcgc gctacgaggg
     4321 ccgtctcttc gacgcgatcg acccggtccc ggtcgactgc gagggcaagt cgccgctgca
     4381 gtgcaaggac gccgcgatcg acgaggtcga gtcgggcacc gcgatcgccg cgctggtgat
     4441 cccggaggac ttcaccgacc ggctcagcgg cgtccaggcg atcgcgctct ccggcccgcc
     4501 gacgatcgag gtcttctaca acgccgacga cccggtcaag gcgtcttacg tcgaggacat
     4561 gatcaaggcc gagctgcgcg acgccaacct cgccctcacc acgcagttca ccgagatcgg
     4621 gctgtcttac ctcgacgtag tcgtctcggg cggccgcgtc gacgtgccgg tgctcgagga
     4681 cctcgacatc ctcgggctcc agcgtgctga gaagatcgtc gagcgggtcc gccccgaggt
     4741 ctctccagag cgccgggccg agctcgacga ggtgatccgc ttctcgcggc tggcgcgcga
     4801 gaacctcgac ctctcgggcc cgctgctgcg cgcgctgcgc gagccgatcc gcgtcgagca
     4861 gacagccctc gacggcggca gcacgtcgct cagctcgttc gccgtcgcgc tcgcggtcgc
     4921 ggtctcgctg atgttcgtca cgctgctgct ggcggccggg acgctcgcgc tcgagcgcga
     4981 ggagaacgcc ttcgcgcggc tcgtgcgggg gctggtctcg cgtacggggc tgcttgtcga
     5041 gaaggcgggg ctggccgcgc tctgctcgtt ggcgatctgc attgcgatgc tcgttgggct
     5101 aggcctgttc gtcgacctcg actggtctcg atcgccgctg tgggtggcgg cgctcgccgt
     5161 cgccgcgatc gcgttcggcg ctatgggcgt cgccgtcggc gccgtcacgc gcgaggtccg
     5221 ggcggcgtcg ctgctctgcg tgatgctctc cctgccgctc gccttcctcg ctctcgttcc
     5281 ctccggctcg gtggccccgg ccgtctacga cgcgacccgc gccgtctccg ccctcttccc
     5341 cttccgcccg acgctcgacg cgctcgacgc cgccctcaac gactcaggcg gcctagtcgc
     5401 cccgctcctc cacctcgctg ccctcgccgc cgccttcgga ttgctcgccc gcctggctct
     5461 gcgccgcttc gcataggtcc gctcgcgccc gccggcgagg cagctgagcg ccgctattgg
     5521 tagttcttgc gctcgaagac gtcgcgggtg agcgagtcga cgcggtcgag gaagaggatg
     5581 ccgtcgaggt ggtcgatctc gtgcagcagg cagcgggcct cgaagccctc ggtctcgata
     5641 cggcgctggg tgacctcgag gccgtcgtcg gtcagcgcca gcgcgtcggc ctcgacgacg
     5701 atcgaggtcg cgcggcggac gttggcggtg aggtggggga tgctgaggca gccctcgcgg
     5761 gcgacctcgg cgccgtcctg ctcgacgacg cgcgggttgg cgagcagcag caggccgctc
     5821 tcgacggtcg ccttcttgtg gccggtgacg tcgaccgcga cgatccgggt caggtcgccg
     5881 agctgggtcg ccgcgagacc tacgcagtga ccgaaggaga ccatcgtctc gagcaggtcg
     5941 cgggcggcgc gctcggtctc ggcgaggtcg ccgacggcca tgtcgtggcc ggccccgccg
     6001 gcgggtgcga cgaccgcggg cgcggcggtc gccttcagcg ctggatgcgg atagagcagg
     6061 acctcgcgga ccgccaccgc gactccctag agctcgtgac tctcgagctc gcgcaggctg
     6121 acctcgacga cctcgctcgc ggcgatctcc gcgagcgcgc tgtcgacatc ggctgggtct
     6181 acgtcgccgg gcaccgcgac ctcgatcagc agcgcgtaga gcgacggcga gccgtcgccc
     6241 tcgaccagcc gggtctcgag gtcggtgatg ttgacgcggt gctcggcgag cacgcgcgtg
     6301 gcgtggtgga tgattcccgg gtggtcgacg ccgtagatgc tcacgacgtg cgtcgcggcg
     6361 ggcacggcct cggcgagcgg ctcgacggcg ctcagcgcga gcgcttccag gccgaggtcg
     6421 gtggccaccg tgtcgaggtc tgcggacagc gccttgcggt cggcgccgtc cggcagcccg
     6481 aggatcagcg tcatcgcgaa gtgcccacgc aggatcgaca tccgcgagtc ctcgacgttc
     6541 acgccgtggc gcagcaggac ccccgtcgtc gccgcgacga tgcccgggcg gtcccggccg
     6601 acggccgata gggcgagctg aggcatggcc tcgcccaccc tagactggtc gcgatgccct
     6661 ttccagcgac ccgcatgcgg cggctgcgcg gtagcggcct gctgcgcgac atggtgcgcg
     6721 agaccgagct gacgccggcc cacctcgttc agccactgtt cgtcgagctc ggcaccgaca
     6781 gccgcacgcc gatcgaggcg atgcccggcg tcgagcgtct gtcgatctcg cacgcggtcg
     6841 aggaggcggg ggagatcaag gagctcggcg tgccggcggt gctgctcttc ggcctgccgg
     6901 ccgacaagga cgaccaaggc tccggcgcct acgacgacga gggcgtcgtc cagctcgcgg
     6961 tacgcgcgat caaggaggcc catcccgagc tgctcgtgat caccgacgtc tgcctctgcg
     7021 cctacaccgc gcacggccac tgcggcatcg tccgccccga cggcgcgatc gacaacgacg
     7081 tcacgctcga gctgctcgcc cagaccgcga tctcgcacgc gcgcgcggga gccgacgccg
     7141 tcgcgccgag cgacatgatg gacgggcgcg tcgccgcgct gcgcggccag ctcgacgccg
     7201 aggacctcaa ggagacgccg atcgtcgcct actccgcgaa gttcgcctcg gccttctacg
     7261 gccccttccg cgaggcagcc gactcgaccc cggcgttcgg cgaccggcgg acctaccaga
     7321 tggatcccgc gaactccgac gaggcggtcc gcgaggcgct gctcgacatg gaggagggcg
     7381 ccgacgtgct gatggtcaag ccggcgctgc cctacctcga cgtgatccag cgcgtcaagg
     7441 aggcgaccgc gatgccgctg gccgcgtaca acgtcagcgg cgagtacgcc atgatcaagg
     7501 ccgccgccgc ggccggtcac atcgacgagc gcgccaccgt gctcgagacg ctgacctcga
     7561 tccgccgggc aggcgcagac atcgtgatca cctaccacgc aaaggacgcc gctcgatggc
     7621 ttcggtgaga gaggctgcgg cgatccccaa ggtccgcagc cgcaaggacg gcgcggcggt
     7681 cccgctcgac gagctcgaca agcgactgct caacctgatg cagggcagct tcctgatcgc
     7741 tccgcgcccc tacgcgcacg tcgccgggct cgccgaggtt tccgaggacg aggtgatgcg
     7801 ccgcgtccag cggctgctcg acgagcggat catccgccag gtcacgccga tcttcgacac
     7861 gcgcgtgctc ggctacagct cgatgctggt cgccgccaag gtcgatccgg agaacccgca
     7921 tcgcgcggcg aagatcgtca actctcaccc cggcgtctcg cacaactacc tgcgcaacca
     7981 cgacttcaac atgtggttca cgatcgccac cgagcccggc tcgaagctcg gcctcgacgg
     8041 cacgctcgac gtcctggcgg agctgaccgg cgccgagtcg gtccgccagc tgccgacgat
     8101 caagctgttc aagatccgga tggacctcga gatggagaag ggcacgcgcg acctcgccgc
     8161 cgcgggcatc gcgcaggatc cgatcgagcc cgacccgatc gagccgaccg agctcgacgt
     8221 cgcggtgatc cgcgcgctgc agggcgacat gccggtgatc cccgagccct acgctcccgc
     8281 ggcggccgag gtcggcatct cgcaggagca gctgctcgac cacctcgagt cgatgcgcga
     8341 gcgcaaggcg ctgcgccggg tcgcggcgat cctcttccac cgccgtgccg gcttctcggc
     8401 gaacggcatg ggcgtctggg cggtgcccga ggagcggatc atggaggtcg cgccgctgat
     8461 ggcctccttc cgcgggatct cgcactgcta ccagcgcccg acctacgacg attggcacta
     8521 ctcggtcttc acgatggccc atggccgctc gaaggaggag tgcgacgcga tcctcgactc
     8581 gatcgccgag gagtccggca tcgacgaccg ccgcacgctc tactcatcga ccgagttcaa
     8641 gaagatccgc ctgctctact tcaccgacga ccaccgccgc tgggaggcgg agcacgctgg
     8701 ctgaggaccg ttcgcgcgcg ctctactagc gcgcagtcga gctgatgccc ggcggggtca
     8761 actcgccggt ccgcgcaatg cgctcgatcg ggcgcgaccc gctcttcatc gccgccggcg
     8821 agggcgcgga gatcgtcgac gtcgacggca accgctacgt cgactacgtc tcctcatggg
     8881 gcccgctgat cgctggccac gcgcatccgc gggtgatcga ggccgtcggc gccgcggcga
     8941 cacggggcac cagcttcggc gcgccgaccg cggccgaggt cgagatcgcg gccgagatcg
     9001 tcgcgcgcgt ccccggcgct gagatggtgc ggatggtcaa ctccggcacc gaggcctcga
     9061 tgagcgcgat ccgcctggcg cgcgcggcga ccgggcgcga gaagctgctc aagttcgccg
     9121 gcgcctacca cggccacgtc gacggcctgc tcgccgacgc cggctcggga ctggcgaccg
     9181 ccggcatccc ggcgagccct ggcgtgccgg ccgcccaggc ggccgcgacg gtcgttgtcc
     9241 cgtggaacga cccgacggcc gtcgagcagg cgctcgccga acacgaggtc gccgcgatcc
     9301 tggccgagcc gtatcccgcg aacatgggct tgatcccgcc ggccgacggc ttcctggcct
     9361 tcctgcgcga gcaggccggg ctggggcggg acgaagccca tgttggccgg atagggctcc
     9421 acgaggaccg ccgcgaactc gtgctcgtcg aaggcggcgc ggaccgctgc ctcgtcgttc
     9481 caggcgacga tcacggtgtt cgccgtcg
//
LOCUS       DATABV010000221         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1199321, whole genome shotgun sequence.
ACCESSION   DATABV010000221 DATABV010000000
VERSION     DATABV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199321"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="VHF88_10300"
     CDS             complement(<1..2313)
                     /locus_tag="VHF88_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2162196.1"
                     /translation="MHQQPPTATPPRPSPPAPEGGAAPPPVGKPKLKKLRLALILSGL
                     GALALVSTVFGMMMAVASDLPQLENKAEYDRAENSTVYADPAAGRDRLARLTGNENRI
                     LLEEDQISPNIANAVIAIEDRRFYDHEGVDYRGIARALFQDILARRAAQGGSTITQQF
                     VKNALAAQSDRSVFQKLREAALAYHLEREWSKQKVLTQYLNSVYFGNGAYGVEAAVRT
                     YFGADDAAPAFDPVTGEPITEDVLDPAGSGDPAATTVSPAEAALLAGLIASPSMYDPV
                     QNPKDSKARRDLVLQRMLEQEMITRSDYEEGIREELPDADDIQPPQPHSNEPYFTTWL
                     TQQLVDRYGPGAVFGGGLDVTTTLDPDLQAAAEQAIAGRIGSIGPAASLVAIENRTGE
                     VKAMVGGPDYQEQPFNLATNGHRQPGSSFKPFILIRALADGMSPDATFASQPKEFPVP
                     GSDSEKFVVNNYEDSYSGVASLWSATANSDNSVYAEIGLDVGTKRVARLANRMGVRTE
                     ISTNPAMTLGGLREGVTPLELAYAYSTIANKGRRVSGTLASSEGGPVAIQSVKGAGRD
                     VENERREERVFPDSVGETAHQLLAGVVSSGTGEAAQIGEFAAGKTGTTENYGDAWFVG
                     FNNELTVAVWVGYPEGLKPMETEYLGGPVAGGTYPAEIWHDLMTAWIGIRDQRLAERE
                     ARQDAGEEDGEVDDTYVPSAPSTPTTPDGVVPEDEGPSDEAAPADPAPDAGGEAPVEP
                     APDPAPEPAPSPAPTPAPAPPPAPSPAPGGG"
     gene            2451..3623
                     /gene="tyrS"
                     /locus_tag="VHF88_10305"
     CDS             2451..3623
                     /gene="tyrS"
                     /locus_tag="VHF88_10305"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX2162197.1"
                     /translation="MTDSAAGDARFLTRNAVEALPEGALERQLAEGRPLRVKLGIDPT
                     APDIHLGHTVVLRKLREFQDIGHTVVLIIGDYTARVGDPSGRSATRPVLSGAQIDANA
                     ETFQRQAFKVLDEQRTEVRRNGEWLDMAMEGLFALARTATVAQLLERDDFAKRHAARE
                     PISLLELLYPLLQGYDSVAVRADVELGGTDQKFNLLLARQIQQAYGVAQQSVLTMPIL
                     PGTDGVRRMSKSLGNYVGVAEPAEEVFGKLMRVPDAAMPVYYELLLDGGLDLERPAVE
                     SKRALARGIAAQLHGQDAARAAEEHFDRLHVARELPDEIPEHAVSANGGAVHLPAVLR
                     DAFGISSSEGRRLLAQGGVKVDGEPFPAGDLDVPAGRLDGAVLQVGKRRFVRLRID"
     gene            4011..>4070
                     /locus_tag="VHF88_10310"
     rRNA            4011..>4070
                     /locus_tag="VHF88_10310"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          558 a         1396 c         1467 g          649 t
ORIGIN      
        1 gccaccgccc ggagcgggcg acggggcagg gggcggcgcg ggcgccggag tgggggcggg
       61 tgacggcgcc ggctcgggcg ccggatcggg cgccggctcg acgggagcct cgccgccggc
      121 gtcgggcgcg gggtcggcag gagcggcctc gtcgctcggg ccctcgtcct cgggcacgac
      181 gccatcgggc gtcgtcggcg tgctcggagc gctcggcacg taggtatcgt cgacctcccc
      241 gtcctcttcg ccggcgtctt ggcgtgcctc gcgctcggcg agccgctgat cgcggatgcc
      301 gatccacgcc gtcatcaggt cgtgccagat ctcagccgga taggtgccgc ccgccaccgg
      361 gccgccgaga tactcggtct ccatcggctt cagcccctcg ggatacccca cccagacggc
      421 aaccgtcagc tcgttgttga agccgacgaa ccaggcgtcg ccgtagttct ccgtcgttcc
      481 ggtcttgccg gcggcgaact ccccgatctg ggccgcctcg ccggttcccg aggagacgac
      541 gccggccagc agctggtggg ccgtctcgcc gaccgagtcg gggaagacgc gctcctcacg
      601 gcgctcgttc tcgacgtcgc gaccggcgcc cttgacgctc tggatcgcga ctggcccgcc
      661 ctccgacgat gccagcgtcc ccgacacgcg ccgtcccttg ttggcgatcg tcgagtacgc
      721 gtaggcgagc tcgagcggcg tgacgccctc tctcaggccg ccgagcgtca tcgcggggtt
      781 tgtcgagatc tcggtcctga cgcccatccg gttggcgagc cgggcgaccc gcttcgtacc
      841 gacgtcgagg ccgatctccg cgtagacgga gttgtcggag ttggccgtgg cgctccacag
      901 cgaggcgacg ccggagtagg agtcctcgta gttgttgacg acgaacttct cgctgtccga
      961 gccgggcacc gggaactcct tcggctgcga ggcgaaggtc gcgtcggggc tcatcccgtc
     1021 ggcgagcgcg cggatcagga tgaatggctt gaacgacgac ccgggttggc ggtggccatt
     1081 ggtcgcgagg ttgaacggct gctcctggta gtcagggcca ccgaccatcg ccttgacctc
     1141 accggtccgg ttctcgatcg cgaccagcga ggccgcggga ccgatcgacc cgatccggcc
     1201 ggcgatcgcc tgctcggccg ccgcctggag gtccgggtcg agcgtcgtgg tcacgtcgag
     1261 cccgccaccg aagacggcgc ccgggccgta gcggtcgacg agctgctgcg tcagccaggt
     1321 cgtgaagtac ggctcgttcg agtgcggctg gggcggctgg atgtcgtcgg cgtccggcag
     1381 ctcctcgcgg atcccctcct cgtagtccga gcgtgtgatc atctcctgct cgagcatgcg
     1441 ctggagcacg agatcgcgcc gcgccttcga gtccttcggg ttctggaccg ggtcgtacat
     1501 gctcggcgag gcgatcaacc cggcgagcag cgccgcctcc gccgggctga ccgtggtcgc
     1561 ggcggggtct ccgctgccgg ccgggtcgag cacgtcctca gtgatcggct cgcctgtgac
     1621 cgggtcgaac gccggggcgg catcgtcggc gccgaagtag gtgcgcacgg cggcctcgac
     1681 gccgtaggcg ccgttgccga agtagacgct gttgaggtac tgggtcagca ccttctgctt
     1741 gctccactcg cgctcgaggt ggtaggccag cgccgcctcg cgcagcttct ggaacaccga
     1801 ccggtcggac tgcgcggcga gcgcgttctt gacgaactgc tgggtgatcg tcgagccgcc
     1861 ctgggccgcg cgtcgcgcga ggatgtcctg gaacagggct cgcgcgattc cgcggtagtc
     1921 gacgccctcg tggtcgtaga agcggcggtc ctcgatcgcg atcaccgcgt tggcaatgtt
     1981 cggcgagatc tgatcctcct cgagcaggat ccggttctcg ttgccggtca gccgcgcgag
     2041 ccggtcccgg cccgcggccg gatcggcgta gacggtcgag ttctcggcgc ggtcgtactc
     2101 ggccttgttc tcgagctgcg gcaggtcgct cgcgaccgcc atcatcatcc cgaacaccgt
     2161 cgagaccagc gccagggcgc cgaggccgct gaggatcagc gccagccgca acttcttgag
     2221 cttcggcttg ccgaccgggg gaggggccgc gccaccctcc ggcgcgggcg gcgacgggcg
     2281 cggaggcgtg gctgtgggcg gctgctggtg catcgcggtc ggtccggggg accagtcctg
     2341 agcggctctg atgccgcggg cccgtcccgg atccgcactg gaaggctcgc gctgggaggg
     2401 tagggagccc ggacggccgg tgcggcgggc gccagtctag cattggcgac atgacggatt
     2461 ccgcggccgg ggacgcccgc ttcctgaccc gcaacgccgt cgaggcgctc cccgaggggg
     2521 cgctcgagcg ccagctggca gagggcaggc cgctgcgcgt gaagctcggc atcgatccga
     2581 cagcgccgga catccacctg ggccacacgg tcgtcctgcg gaagctgcgc gagttccagg
     2641 acatcggcca caccgtcgtg ctgatcatcg gcgactacac cgcgcgcgtg ggcgacccat
     2701 caggacggtc ggcgacccgc ccggtgctgt ccggcgcgca gatcgacgcc aacgccgaga
     2761 ccttccagcg ccaggccttc aaggtgctcg acgagcagcg gaccgaggtc cgccgcaacg
     2821 gcgagtggct cgacatggcg atggaggggc tgttcgcgct cgcccggacc gcgaccgtcg
     2881 cccaactgct cgagcgcgac gacttcgcca agcgccatgc cgcccgtgag ccgatctcgc
     2941 tgctcgagct gctctacccg ctgcttcagg gctacgactc ggtcgccgtg cgcgcggacg
     3001 tcgagctagg ggggacggac cagaagttca acctgctgct cgcccgccag atccagcagg
     3061 cctacggggt ggcgcagcag tcggtgctca cgatgccgat cctgcccggc accgacgggg
     3121 tccggcggat gtcgaagtcg ctcggcaact acgtcggcgt cgccgagccc gccgaggagg
     3181 tcttcggcaa gctgatgcgc gtcccggacg ccgcgatgcc cgtctactac gagctgctgc
     3241 tcgacggcgg gctcgatctc gagcgcccgg cggtcgagtc taagcgcgcg ctggcgcggg
     3301 ggatcgcggc ccagctgcac gggcaggatg cggcgcgggc ggccgaggag cacttcgacc
     3361 gcctgcacgt cgcgcgcgag ctgccagacg agattcccga gcatgccgtc tctgccaacg
     3421 gcggcgcggt ccatctgccc gccgtattgc gcgacgcctt tgggatctcg agctccgagg
     3481 gccgcaggct gctggcccag ggaggggtca aggtcgacgg ggagcccttc ccggccggcg
     3541 acctcgacgt gcccgcgggc cgtctcgacg gcgccgtgct gcaggttggc aagcgccgtt
     3601 tcgtacgctt gcgaatcgat tgagcgctgg ccgtgacaat ccgccggcgc agcgctatac
     3661 tcccctgtcc cttcggatga gcaagatcac ggagacgcca ggcgcctagc cctctgtcgc
     3721 aagatggaga gcggtgctgg gcctctacgg aggcctttct catgtgaagg ggacgtattc
     3781 gaacggcgga gtccccgccc gagggtgcgg cggtctttga aaactcaaca gcatgtgcga
     3841 ccggatccgc gaggatcttg gtcgcgtcca ggttcgaccc tgcgcgaccg gctctgccgg
     3901 tgggcgtggg gcttaactga gtaggtagta gaacccgtcg atccgagggt cccgcaacgt
     3961 gtacgcaggg cccgcgcagt acggatacga cagctttcca tgaagcattc ttcacggaga
     4021 gtttgatcct ggctcaggac gaacgctggc ggcgtgctta acacatgcaa
//
LOCUS       DATABV010000222         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_157105, whole genome shotgun sequence.
ACCESSION   DATABV010000222 DATABV010000000
VERSION     DATABV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157105"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..669
                     /gene="pyrR"
                     /locus_tag="VHF88_10315"
     CDS             109..669
                     /gene="pyrR"
                     /locus_tag="VHF88_10315"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX2162198.1"
                     /translation="MSEKVVLERDDLRRTLRRIAHEIAEKNPIPDGLAIVGIHTRGAV
                     LARRLHALLGEMTESEVPIGDLDISFYRDDVGAKSPASQPVVHSSHVDFDIADRTVVL
                     VDDVLFTGRTVRAAIDALFDYGRPRRVQLAVLCDRGHRELPIRPDYVGKNLPTARGER
                     VNVRLEEVDEVDEVTITEPRTVEALA"
     gene            666..1628
                     /locus_tag="VHF88_10320"
     CDS             666..1628
                     /locus_tag="VHF88_10320"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX2162199.1"
                     /translation="MRHLLAVEDLSRAEIEAILDRAESFAEVARRDIKKVPTLRGRTV
                     VSLFYEASTRTSSSFELAAKRLSADLVSIKAGGSSVDKGESLKDTVATLSAYDPAAIV
                     IRHPYAGAAQLVAGWSEAAVINAGDGKHEHPSQALLDLYTLRRRLGSLDGKRIWIVGD
                     VLHSRVARSNLLAFGAMGAEVTVCGPPTLIPRGIEALGCRVAHSLEQMGEADVVYALR
                     LQKERMTESFLPSLREYAVHFQIDTRRLAPRQLLMHPGPVNRGVELSGEAVDSPQSLI
                     SEQVASGLVVRMAILYELLAARDRPAPVEGEGAEASPTPLGQPA"
     gene            1625..>2714
                     /locus_tag="VHF88_10325"
     CDS             1625..>2714
                     /locus_tag="VHF88_10325"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX2162200.1"
                     /translation="MSIAAALDMRSGEGANVVLRGATVLDPVAGIEGVHDVVVRAGKI
                     AELVAPGAGEAGGGEEIDAEGLHAFPAFFDPHVHLRTPGQEHKEDLETGTRAAAAGGY
                     CGVLAMANTDPPVSTPADVEALREAAREQASVPVGFLATVTRGMEGRELTEMAALRDA
                     GAIGFSDDGLPVRSARVLRRALQYQRLCGGTIALHEEDSELSGNGVMHEGPVSAALGL
                     TGIPSVSESTTIARDAALAAYEEARIHVQHLSAAESVDAVRAAKAVGVRISAEASPHH
                     LCLVDEAVRSLDASRFKMNPPLRAEADRQALLDGLRDGTIECIATDHAPHATEEKEVP
                     FEQAAMGVTGLETAFAALHTYLVVPGVLD"
BASE COUNT          378 a         1011 c          953 g          372 t
ORIGIN      
        1 ggggccgcct tcgggcggcc ccgattagtt gtgactccat cccggcccgg tgctaggctg
       61 acggccgtcc tttaaccagg tcccgtgagg ccaggaagga gcaacccagt gagcgagaag
      121 gtcgtgctcg agcgcgacga tttgaggcga acgctgcggc ggatcgccca cgagatcgcc
      181 gagaagaacc cgattcccga cggcttggcg atcgtcggca tccatacgcg gggcgccgtc
      241 ctggcccgcc ggctccacgc cctgctcggc gagatgaccg agagcgaggt tccgatcggg
      301 gacctggaca tttccttcta ccgcgacgac gtcggcgcga agtcgccggc ctcgcagccg
      361 gtcgtccact cgtcgcacgt cgacttcgac atcgcggacc gcacggtcgt cctcgtcgac
      421 gacgtcctct tcaccggtcg cacggtgcgc gccgcgatcg acgccctctt cgactacggc
      481 cgcccgcggc gcgtgcagct tgccgtcctc tgcgaccgcg ggcaccgcga gctgccgatc
      541 cgccccgact acgtcggcaa gaacctgccg accgcgcgcg gcgagcgggt caacgtgcgg
      601 ctcgaggagg tcgacgaggt cgacgaggtc acgatcaccg agccgcggac ggtggaggcg
      661 ctggcatgag gcacctgctc gcggtggagg acctctcgcg ggccgagatc gaggcgatcc
      721 tcgaccgtgc cgagagcttc gcggaggtgg cccggcgcga catcaaaaag gtgccgaccc
      781 tgcgcgggcg gaccgtcgtc agcctcttct acgaggcgag cacgcgcacc tcgtcctcct
      841 tcgagctcgc cgccaagcgg ctctccgccg acctcgtctc gatcaaggcg ggcggctcct
      901 cggtcgacaa gggcgagtcg ctcaaggaca ccgtcgcgac gctgtccgcg tacgacccgg
      961 ccgcgatcgt catccgccac ccctacgcgg gcgccgccca gctcgtcgcc ggctggtcgg
     1021 aggccgcggt catcaacgcc ggggacggta agcacgagca ccccagccag gccctgctcg
     1081 acctctacac gctgcgccgc cggctcggct cgctcgacgg caagcggatc tggatcgtcg
     1141 gtgacgtcct gcacagccgc gtcgcgcgct ccaacctcct cgccttcggg gcgatgggcg
     1201 ccgaggtcac cgtctgcggc ccgccgaccc tgattccgcg cgggatcgag gcgctcggct
     1261 gccgggtcgc ccactcgctg gagcaaatgg gggaggcgga cgtcgtctac gcgctgcggc
     1321 tgcagaaaga gcggatgacc gagagcttcc tgccctcgct gcgcgagtac gcggtccact
     1381 tccagatcga cacccgccgg ctcgctcctc gccagttgct catgcatccc gggccggtca
     1441 accgcggcgt cgagctctcc ggagaggcgg tcgactcgcc gcagtcgctg atctccgagc
     1501 aggtggcctc cggcctggtc gtgcggatgg cgatcctcta cgagctgttg gcggcacgcg
     1561 accggccggc gccggtcgag ggcgagggcg ccgaggcctc tccgaccccc ttgggacagc
     1621 cggcgtgagc atcgccgcgg cgctcgacat gcgctccggc gagggcgcga acgtcgtcct
     1681 gcgcggcgcc accgtgctcg acccggtcgc tgggatcgag ggcgtccacg atgtcgtcgt
     1741 ccgcgccggc aagatcgccg agctcgtcgc tccaggcgcc ggcgaggcgg ggggcggcga
     1801 ggagatcgac gccgagggcc tgcacgcgtt ccccgccttc ttcgaccccc acgtccacct
     1861 gcggacgccg ggtcaggagc acaaggagga cctcgagacc ggcacccgtg ccgcggccgc
     1921 cggcggctac tgcggcgtgc tcgcgatggc caacaccgat ccgcccgtct cgacccccgc
     1981 cgacgtcgag gcgctgcgcg aggcggcccg cgagcaggcc tcggtgccgg tcggcttcct
     2041 cgccaccgtc acccgcggca tggaggggag ggagctgacc gagatggcgg cgttgcgcga
     2101 cgccggcgcg atcggcttca gcgacgacgg gctgcccgtc cgcagcgctc gggtgctgcg
     2161 gcgcgccctc cagtaccagc ggctctgcgg cggcacgatc gccctccacg aggaggactc
     2221 cgagctctcg ggcaatggcg tcatgcacga gggcccggtt tcggcggcgc tcggcctgac
     2281 gggcattccc tcggtctcgg agtccacgac gatcgcccgc gacgcggcgc ttgccgccta
     2341 cgaggaggcg cgcatccacg tccagcacct ctcggccgcc gagtccgtcg acgccgtgcg
     2401 ggccgccaag gcggtcggcg tgcggatcag cgccgaggcc agcccgcacc acctctgcct
     2461 cgtcgacgag gcggtgcgga gcctcgacgc gagccgcttc aagatgaacc cgccgctgcg
     2521 cgccgaggcg gatcggcagg ccctcctcga cgggctgcgc gacggcacga tcgagtgcat
     2581 cgccaccgac cacgccccgc acgcgacgga ggaaaaggag gtgccgttcg agcaggcggc
     2641 gatgggggtc accgggctcg agaccgcctt cgcggcgctc cacacctacc tggtcgtgcc
     2701 cggcgtgctc gacc
//
LOCUS       DATABV010000223        11700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1361172, whole genome shotgun sequence.
ACCESSION   DATABV010000223 DATABV010000000
VERSION     DATABV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361172"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2506)
                     /gene="alaS"
                     /locus_tag="VHF88_10330"
     CDS             complement(<1..2506)
                     /gene="alaS"
                     /locus_tag="VHF88_10330"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX2162201.1"
                     /translation="MNAAEIRERYLSFFEARGHRRMPSASLVPASYDPSVLLTTAGMQ
                     PFKPYFRGEEQPPAPRLTSCQKCFRTTDIENVGLTARHLTFFEMLGNFSIGDYFKREA
                     IELAWELSTSADGYGFDPAQVWVTVFGGDRELGLDEDAEAVAFWQAIGVPDERIVRLG
                     RDDNFWQAGPTGPCGPCSELYLDRGVDFGSAEDRPGDDSERFLEFWNLVFMQYELHGD
                     GSLTDLPQRNIDTGLGLDRMAAILQDVPSVYETEHFRPLIEFGEQRSGRSYGDDFATT
                     RALRVLADHGRAAAFLIADGVVPSNEDRGYILRRILRRAIHQGRVLGIEQGFLAQLCD
                     VVVDALGDVYGELRSERETIRRWTAAEEEGFGRTLANGQRLLDELIVAARDREAGEIA
                     AADAFKLHDTYGFPFELTQELLAEQGLTVDVAGFESLMDDARATARAGARETDGGDTE
                     RVAELVRAAGFQTNFVGYERTDAETVARTVERLNGTLLVKLEDSPFYPQGGGQVSDSG
                     FVETPTARGRVVDVVRVGADQALAVEPVEGEIAVGDAVHAVVERAERLATMRNHTATH
                     LLHAALRERLGAHVRQAGSYVGPDKLRFDFTHGERLSAEELRAVEQRVNGWIADGLAV
                     RAIETTLDEARALGAMALFGEKYGDWVRMVEVADVSRELCGGTHVASTAEIGLFHVTT
                     ETSSASNVRRIEAVTGPAATALFEQRTAQLRELSGLLKAPEQELGRAAERLIERSKQD
                     KRRSAGGDDGAEFEALTAGAQHVGGIDVVTARSAGVDDPKALLALSDRVAQKLGDAVV
                     VLGADFDGKVHLVVQSTPVAVERGVKAGEVVRAAAEV"
     gene            2803..4209
                     /locus_tag="VHF88_10335"
     CDS             2803..4209
                     /locus_tag="VHF88_10335"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX2162202.1"
                     /translation="MIVAEQLVGEARERRQRSMSHLERIVYAISAIAFVATAVSIAAF
                     MPDERPSDALLACALMAGYAIVSRVRFEFGWGYVTPEHLVFIPMLLLLPLPWVPFFVV
                     GGTLLAQLPDFVDRSWHRDRWVGVFPDSWFCIGPVLVLGALAPGTADFSMVGVYALAF
                     AAQVLFDFSWGAISARVLDHLAGKYPVPLVEIWHNYVGVIRFDGLLTPVAIAIAILAV
                     QEPLALLAIGPLVLMLESFARERSDRYSKALELQRAYRGTVMLLTDVVESEDSYTADH
                     SRSVVELVNAVADEMNVDARDRLELEFAAMLHDVGKIAIPKEILNKPAKLTDAEFTVM
                     KQHTIEGQHMLDRVGGLLGTVGEIVRSCHERWDGGGYPDGLAGEQIPLAARIVFTCDA
                     YNAMTTDRVYRAALPVEEALRELEVNAGTQFDPRVVAALTRVVERSDPQPFTAADDVR
                     AVLAADSAITAQRVGAAV"
     gene            complement(4093..5469)
                     /locus_tag="VHF88_10340"
     CDS             complement(4093..5469)
                     /locus_tag="VHF88_10340"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2162203.1"
                     /translation="MRATFDLLRHERRARIFFAVLLQSALGTGAGYVALLLIAYDRYE
                     SPWAISLVLLAELLPAMLLGPVFGALADRWSRKGCMVVGDVLRSLAFVGVVAVDSFEA
                     TVLFAGLAGVGTALFNPAALAALPSVVDEPRRAPAMTSLYGVAGDFGFTGGFALSASL
                     LLVLGGPAGLMVVSATTFAISALLLARLRFGAIPARHADGSHPTLAQEIKEGVRATVG
                     MRDVRTVLVGSAGGLVCAGVFNVAELPFVTEDLGASDAGFSLLVMFFGIGFVAGSLAG
                     SRGGRPSRLKRRYLAGLLLLGVGLVTTALAESYIVVALTFAVAGFGNGLLLVYERLLI
                     QAAVPDRLVGRIFGAKDALTAWAFGVAFVAGGALVSGLGPREPILLAGAAALVVYVVT
                     ALALRGEWTAEGPVEGGPLVAAGPGSALLDGGANALGRDGAVGSQDGADVVGGGEGLR
                     VGPLDDPR"
     gene            complement(5801..7438)
                     /locus_tag="VHF88_10345"
     CDS             complement(5801..7438)
                     /locus_tag="VHF88_10345"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2162204.1"
                     /translation="MSEPLLSVRDLSVAFEGERGTLRAVDGLSLDLAPGEVLALVGES
                     GSGKSVTALSLIGLLRAPGARIAGSARYGGSELIGAPERELRRLRGEELAMVFQDPLS
                     SLNPVLRVADQVAEQVLAHRDVSAREARERAVALLRRVGIPEPERRADAYPHELSGGM
                     RQRVMIAMALSCDPRILIADEPTTALDVTVQAQILELIRALRAETGAGVLLITHDFGV
                     VAELADRVAVMHEGRIVEQGVVEDVFERPESPHTRRLLAAVPRLDGPLREPRTFGDRP
                     PVLELADVEVSFPVRRRLLGRGGERRRAVDGVTLSIAAGETVGLVGESGSGKSTLART
                     AVRLLEPTAGSVSFDGRDITRARRRELTPLRADLQIVFQDPYGSLNPRKRARDIVGLP
                     LRLGGVPRSEVGPRVERLLERVGLKAAHADRWPHELSGGQRQRVGIARALALSPRLVV
                     LDEPVSALDVSVQAQILDLLGELQRELGLSYLFISHDLRVVRQVSDRVAVMRDGRVVE
                     LAPVADLYARPQHPYTAELLAAVPASRPVHRSASTRS"
     gene            complement(7435..8382)
                     /locus_tag="VHF88_10350"
     CDS             complement(7435..8382)
                     /locus_tag="VHF88_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2162205.1"
                     /translation="MTAFVARRLAQMVVVLFAVSVLTFLIFNVIPNGDPAARMAGKSP
                     TESQVEAIRAEWGFDEPLPVQYVRTMEQVLGGSAISYFTQLNVREEIVRGLPRTLSLA
                     IGAAILWMAFGVGLGLLGAMRAGRASDRLLTLLALVGVSLPVFWLGALLSHYLGFKAG
                     WFPNGGYVPLTEDPLDWAYHLVLPCATLAVLFIGFYSRILRSNVLDTMGEDYVRTARA
                     KGLSEREVLRRHVLRNSLIPVVTLWGLDFGAVVAGGAILTEVVFDLQGVGQYAAESVG
                     QLDVPPVLAVTLFGAFFIVVTNTVVDIVYAALDPRIRVT"
     gene            complement(8379..9350)
                     /locus_tag="VHF88_10355"
     CDS             complement(8379..9350)
                     /locus_tag="VHF88_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2162206.1"
                     /translation="MGQSPWRLALRRLRRDRVALGFGALFVALVLAALAAPLWASAVA
                     DRQPDRTNETGKIEVDGRQVDVVSLEGVPIGPQWRGEYFLGADANGRDVMVRLLYGAR
                     NSLLIGLAAALLTALLATMAGLVAGYFGGWVDAVARSTLDVIWAFPVILLGVALGTAL
                     AVGGLQVGPLQLEAGSMAISILVIAVVYVPYMARPIRGEVMALREREFVEAARAQGAG
                     PLRTMATELLPNLTSTIIVFAPLMVANAILLEAALSFLGVGVVPPDPSWGNMIDSGVE
                     KLITAPHLAIAPGAMLVLTVLALNVFGDGVRDAFDPRSSAAWGTRRL"
     gene            complement(9365..11014)
                     /locus_tag="VHF88_10360"
     CDS             complement(9365..11014)
                     /locus_tag="VHF88_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2162207.1"
                     /translation="MTATRSSLTVLALALTAAIGGGCGSDSDGDQASGGKQGGAITIA
                     QGSQPDALDPALAYTSNSWEPLWLVYTPPLTYRRAEGVEGAELIPGAAEALPDVSADG
                     KTYSFTIRKGLKYSDGTPVKASDFEHAIKRVLNLESGGSYLFEGIDGATEYVEAGEAG
                     GDIAGIEANDRTGEVTVRLTAPDGSFNNALATTFAGLVPASTPFENQTQSPPPGVGPY
                     AITESTPNRQFVMEKVEGFDIPGIPQGNLDTITTKIVKSSSRQAQDVIDGELDYMQDA
                     PPTDLTAEIKARYSDRYKEWPTLSTNYFFLNQSLPPFDDEQVREAVNVALDSTAIARL
                     FGGRLAPTCNLIPPGIPGQAELDPCPFGDPNGPGDIRRAKQLVEQAGATGDQVTVYAN
                     NDTNRPQIGEYYTALLNDIGLDAELRVIDGAVYFNTIGKESTKAQTGLASWYADFPHP
                     ASFMQQLDGTTIQPTNNTNFGYVDDPVINRGVAELSQEPDAIEVADRWAELERRAIER
                     SYVAPYGSEELTTFLSERMDAENCSLVHPLYGNDYSSFCLK"
     gene            complement(11213..>11700)
                     /locus_tag="VHF88_10365"
     CDS             complement(11213..>11700)
                     /locus_tag="VHF88_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162208.1"
                     /translation="PFGDRDGDADLVEASRLVADAGARGAAVAVAAGGDARGRRLARY
                     LAATLRKIGLAARVTRRAGAQVAFAAISPTIPHPSRYLAAVDDPVLAARAELLEQEGD
                     AADLEQDWAAVDEEVVTRAYGAPYGVATAGVLASRRLDMQGCARFHPVVGMDYSSACL
                     R"
BASE COUNT         1758 a         4250 c         4049 g         1643 t
ORIGIN      
        1 cgacctcggc ggccgcccgc acgacctccc cggccttgac gccgcgctcg acggccaccg
       61 gcgtcgactg gacgaccagg tggaccttgc cgtcgaagtc cgctccgagc accacgaccg
      121 catcgccgag cttctgcgcg acgcggtcgg acaacgcgag cagcgccttc gggtcgtcca
      181 cgcccgccga gcgcgcggtc acgacgtcga tgcccccgac gtgctgcgcg ccggcagtca
      241 acgcctcgaa ctctgcgccg tcgtcgccgc ctgcggaccg ccgcttgtcc tgcttggacc
      301 gctcgatcag ccgctcggcg gcgcgcccga gctcctgctc gggcgccttg agcagccccg
      361 acagctcgcg cagctgtgcg gtccgctgct cgaacaacgc ggtcgcggcc ggccccgtga
      421 cggcctcgat gcggcgcacg ttcgatgccg acgaggtctc cgtcgtgacg tggaacaagc
      481 cgatctcagc ggtgcttgcg acgtgggtcc cgccgcacag ctcacgcgac acgtcggcga
      541 cctcgaccat ccgcacccag tctccgtact tctcgccgaa cagtgccatc gctccgagcg
      601 cgcgcgcctc gtcgagcgtg gtctcgatcg cccggacggc gagcccgtcc gcgatccagc
      661 cgttgacgcg ctgctctact gcccgcagct cctccgccga cagccgctcg ccgtgggtga
      721 agtcgaagcg cagcttgtcg ggcccaacgt aggagccggc ttggcggaca tgggcgccga
      781 gccgctcgcg cagcgccgcg tgcagcagat gggtggcggt gtggttgcgc atcgtcgcga
      841 gccgctcggc gcgctcgacg acggcgtgga cggcgtcgcc gacggcgatc tcgccttcga
      901 cgggctcgac cgctagcgcc tggtcggctc cgacgcgcac gacgtcgacc acgcgcccgc
      961 gcgccgtcgg cgtctcgacg aagccgctgt cggagacctg gccgccgccc tgggggtaga
     1021 acgggctgtc ctcgagcttg accagcagcg tgccgttgag ccgctcgacc gtgcgggcga
     1081 cggtctcggc atcggtccgc tcatagccga cgaagttggt ctggaagccc gccgcgcgga
     1141 cgagctcggc gacgcgctcg gtatcgccgc cgtcggtctc gcgcgcgccg gcgcgcgccg
     1201 tcgcccgcgc gtcgtccatc aatgactcga agcctgcgac gtcgaccgtc aggccctgct
     1261 cggcgagcag ctcctgcgtc agctcgaagg ggaagccgta ggtgtcgtgc agcttgaaag
     1321 cgtcggccgc ggcgatctcg ccggcctcgc ggtcgcgcgc ggcgacgatc agctcgtcga
     1381 gcagtcgctg gccgttggcg agcgtgcggc cgaagccctc ctcctcggcg gcggtccagc
     1441 ggcggatcgt ctcgcgctcg gagcgcagct cgccatagac gtcgccgagc gcgtccacca
     1501 cgacgtcgca gagctgcgcc aggaagccct gctcgatccc cagcacgcgg ccctggtgga
     1561 tcgcgcggcg caggatccgc cgcaggatgt agccgcggtc ctcgttcgac gggacgactc
     1621 cgtcggcgat caggaacgcg gccgcgcggc cgtggtcggc gagcacgcgc agcgcacgcg
     1681 tcgtcgcgaa gtcgtcgccg tagctgcgtc ccgaccgctg ctcaccgaac tcgatcagcg
     1741 gccggaagtg ctcggtctcg tagaccgacg ggacgtcctg gaggatcgcc gccatccggt
     1801 cgaggcccag gccggtgtcg atgttgcgct gcggcaggtc ggtcagcgag ccgtcgccgt
     1861 gcagctcgta ctgcatgaag acgaggttcc agaactcgag gaagcgctcg ctgtcgtcgc
     1921 cggggcggtc ttccgcgctg ccgaagtcga cgccgcgatc gaggtagagc tcggagcacg
     1981 gcccgcacgg cccggtcggg cccgcctgcc agaaattgtc gtcgcggccc agccgcacga
     2041 tccgctcgtc gggaacgccg atcgcctgcc agaacgccac cgcctcggca tcctcgtcga
     2101 ggccgagctc gcggtcgccg ccaaagaccg tcacccagac ctgcgcggga tcgaagccgt
     2161 agccgtcggc cgacgtcgac agctcccagg cgagctcgat cgcctcgcgc ttgaagtagt
     2221 cgccgatcga gaagttgccg agcatctcga agaacgtgag atggcgcgcg gtcaggccga
     2281 cgttctcgat gtcggtcgtg cggaagcact tctggcacga ggtcaggcgc ggcgccggcg
     2341 gctgctcctc gccgcggaag tacggcttaa acggctgcat gccggcggtc gtgagcagca
     2401 cgctggggtc gtagctcgcc ggcacgagcg aagccgacgg catccgccgg tgcccgcgcg
     2461 cctcgaagaa cgacaggtag cgctcgcgaa tctccgctgc gttcaccgga cccagtctag
     2521 gaattcagcc ggcgaggcac cgccgggggc gcgcggcgtg gaacctgtcc gcagccggcc
     2581 aggcaccggc ggggggcgcg gggggcgcga gacccccgat cacacttcgt tgcaggacca
     2641 caagccggcg gcgcgtatag ctcagcattt gacaactttc gctgcacgac gaggcccggc
     2701 cggcggcgaa cctgcgcgcc gagcgtcggc tcggcggcct gaacctctgc aatgtcgcgc
     2761 agcggcgatt gggcgtccca cggggacccg acgataagtc ggatgatcgt cgccgagcaa
     2821 ctggtcggtg aggcacgcga gcgccgacag cgctcgatgt cgcatctcga gcggatcgtc
     2881 tacgcgatct cagcgatcgc gtttgtcgcg accgcggtct cgatcgcggc gttcatgccc
     2941 gacgagcgcc cgtccgacgc actgctggcc tgcgcactga tggccggcta cgcgatcgtc
     3001 tcgcgcgtcc gcttcgagtt cggctggggc tacgtcacac ccgagcacct ggtcttcatc
     3061 ccgatgctgc tgctgctgcc gctgccgtgg gtgcccttct tcgtggtcgg cggcacgctg
     3121 ctggcccagc tacccgactt cgtcgaccgc tcctggcacc gcgaccgctg ggtcggcgtc
     3181 ttcccggact cctggttctg catcggcccg gtgctcgtgc tcggcgcgct ggctcccggg
     3241 acggccgact tctcgatggt cggcgtctac gccctcgcct ttgccgccca ggtgctgttc
     3301 gacttctcct ggggcgccat ctcggcgcgc gtcctcgatc atctcgccgg caagtacccg
     3361 gtgccgctcg tggagatctg gcacaactac gtcggcgtga tccgcttcga cggcctgctc
     3421 accccggtcg cgatcgcgat cgcgatcctt gccgtacagg agcccttggc gctgctggcg
     3481 atcggcccgc tcgtcctgat gctcgagagc ttcgcgcgcg agcgcagcga ccgctactcg
     3541 aaggcgctcg agctgcagcg cgcctaccgc ggcaccgtga tgctgcttac cgacgtcgtc
     3601 gagtccgagg actcctacac ggccgaccac agccgctcgg tcgtcgaact ggtgaacgcg
     3661 gtggccgacg agatgaacgt cgacgcgcgc gaccgcctcg agctcgagtt cgccgcgatg
     3721 ctgcacgacg tcggcaagat cgcgatcccc aaggagatcc tcaacaagcc cgcgaagctg
     3781 accgacgccg agttcacggt gatgaagcag cacacgatcg agggccagca catgctcgac
     3841 cgggtcggcg gcctgcttgg gacggtcggc gagatcgtcc gctcctgtca cgagcgctgg
     3901 gacggcggcg gctatcccga cggcctcgcg ggcgagcaga tccccctcgc ggcgcggatc
     3961 gtcttcacat gcgacgccta caacgcgatg acgaccgacc gcgtctaccg cgccgcgctg
     4021 cccgtcgagg aggcgctccg cgagctcgag gtcaacgccg gcacgcagtt cgacccgcgc
     4081 gtcgtggcgg cgctaacgcg ggtcgtcgag cggtccgacc cgcagccctt caccgccgcc
     4141 gacgacgtcc gcgccgtcct ggctgccgac agcgccatca cggcccagcg cgttggcgcc
     4201 gccgtctagc agcgcgctcc ccggtcccgc cgcgaccagc ggtccgccct cgaccggccc
     4261 ctcggcggtc cactcgccgc gcagggcgag cgctgtcacg acgtagacga ccagggctgc
     4321 cgcgccggcc agcaggatgg gctcgcgcgg gccgagcccg ctgaccaggg cgccgccggc
     4381 gacgaaggcg acgccgaacg cccaagcggt cagggcgtcc ttcgcgccga agatgcgccc
     4441 gaccaggcga tccggcaccg ccgcctggat cagcagccgc tcgtagacga gcagcaagcc
     4501 gttgccgaag cccgcgaccg cgaaggtcag cgcgaccacg atgtagctct cggcgagagc
     4561 ggtcgtgacc agcccgacgc cgagcagcag caggccggcg aggtaccgcc gcttgagcct
     4621 cgagggcctg cccccgcgcg accccgccag cgaccccgcg acgaagccga tgccgaagaa
     4681 catcaccaac agcgagaagc cggcgtcgct ggctcccagg tcctcggtga cgaatgggag
     4741 ctcggcgacg ttgaagactc cggcgcagac caggccgccg gccgagccga ccaggacggt
     4801 cctcacgtcg cgcatgccga ctgtggcgcg cacgccctcc ttgatctcct gcgccagcgt
     4861 cggatggctt ccgtcggcgt ggcgtgcggg gatcgccccg aagcgaagcc gagcgagcag
     4921 cagagcggag atcgcgaagg tcgtcgcgct gacgaccatg agacccgccg gaccgccgag
     4981 caccagcagc aggctcgcgc tcagggcgaa gccgccggtg aagccgaagt cgcccgcgac
     5041 gccgtagagc gaggtcatgg ccggagcccg acgcggctcg tcgaccacgc tggggagcgc
     5101 ggcgagggcg gccggattga agagcgcggt gccgaccccc gccagtcccg cgaacaggac
     5161 cgtggcctcg aagctgtcga cggcgaccac gccgacgaac gcgagcgacc gcaggacgtc
     5221 gcccaccacc atgcagccct tgcgcgacca gcggtcggcg agcgcgccga agacgggccc
     5281 cagcagcatc gccggcagca gctccgccag caacacgagg ctgatcgccc acggcgactc
     5341 gtagcggtcg taggcgatca gcagcagtgc tacatagccg gctccggtcc cgagcgccga
     5401 ctgcagcagc accgcgaaga agatccgcgc gcggcgctcg tgtcgaagga ggtcgaaggt
     5461 cgctcgcata tgagcgcgct gagactagcc gtgaaagccg ggcgcggcca tccgagcccg
     5521 gaaatgacag ggccgcgccc ggaggcacgg ccctgtactg cgattgcttc gcgaattctg
     5581 attagcgctt gagcggcatt ctggctacct cctggaatct cttcagggca cccctcgcgt
     5641 caatgcatag gttcttcggc gagaacccgt gcgtgatgca ggcacagcat ggcacatagt
     5701 gcggtcatgc gccaatttgc atgcacgaag ttgtgcaaag agaggacagc cgtcgctcac
     5761 ggagacgctt cggcgctgac tggcgttgct cggggccgcg ctacgagcgg gtgcttgcgc
     5821 ttctgtggac cggccgcgag gccggcacgg cggccagcag ctccgcggtg tacgggtgct
     5881 gcggccgggc gtagagatcg gcgacgggcg cgagctcgac caccctgccg tcgcgcatca
     5941 cggcaacgcg gtcggagacc tggcggacga cgcgcaggtc gtgggagatg aagaggtagg
     6001 acagcccgag ctcgcgctgg agctcgccga gcaggtcgag gatctgagcc tggacggaga
     6061 cgtcgagcgc cgagaccggc tcgtctagca cgacgaggcg cgggctcagc gccagcgcgc
     6121 gagcgatccc gacccgttgg cgttgaccgc cggacagctc gtgcggccag cggtcggcgt
     6181 gcgcggcctt gagccccacg cgctcgagca gccgctcgac gcgcggtccg acctcggacc
     6241 gcgggacgcc gcccagccgc agcggcagac cgacgatgtc gcgcgcgcgc ttgcgcgggt
     6301 tcagcgaccc gtatgggtcc tggaagacga tctgcaggtc cgcgcgcagc ggcgtcagct
     6361 cgcgccggcg cgctcgcgtg atgtcacgtc cgtcgaagct cacgctgccg gccgtcggtt
     6421 ccagcaggcg cacggccgtt cgcgcgagcg tcgacttgcc tgagcccgac tcgccgacga
     6481 gcccgacggt ctcccccgcc gcgatcgaga gcgtcacgcc atcgacggcg cgccggcgct
     6541 cgccgccgcg gcccagcagg cgccgccgga ccggaaagct gacctcgacg tcggcgagct
     6601 ccagcaccgg cggtcggtcg ccgaaagtcc gcggctcgcg cagcggtccg tcgaggcgcg
     6661 ggacggcggc gagcagccgg cgcgtgtgag ggctctccgg ccgctcgaag acgtcctcca
     6721 ccacgccctg ctccacgatc cgcccctcgt gcatcaccgc gacgcgatcg gccagctcgg
     6781 cgacgacgcc gaagtcgtgg gtgatcagca gcaccccggc cccggtctcc gcgcgcagcg
     6841 cgcggatgag ctcgaggatc tgcgcctgga ccgtcacgtc gagcgccgtc gtcggctcgt
     6901 cggcgatcag gatccgcgga tcgcaggaga gcgccatcgc gatcatcacg cgctgacgca
     6961 ttccgcccga gagctcgtgc ggataggcgt cggcgcgccg ctcgggctcc gggatgccga
     7021 cgcggcgcag cagcgccacc gcccgctcgc gcgcctcgcg ggcgctgacg tcgcggtggg
     7081 ccaggacctg ctcggcgacc tggtccgcta cgcgcagaac ggggttcagc gatgagagcg
     7141 ggtcctggaa gaccatcgcg agctcctccc cgcgcagcct gcgcagctcg cgctccggcg
     7201 ctccgatcag ctcgctgccg ccgtagcgcg cgctgccggc gatccgcgcg ccgggcgccc
     7261 gcagcagccc gatcagcgac aacgcggtga ccgacttgcc cgagcccgac tccccgacca
     7321 gcgcgaggac ctcgcccggc gcgaggtcga gcgagagccc gtcgacagcg cgcagcgttc
     7381 cgcgctcgcc ctcgaacgcg accgagaggt cgcgcacgct cagcagcggc tcgctcacgt
     7441 cacccggatc cgcggatcga gtgccgcgta gacgatgtcg acgaccgtgt tcgtcacgac
     7501 gatgaagaac gctccaaaca gcgtcaccgc gagcaccggc ggcacgtcga gctgtccgac
     7561 cgactcggcc gcgtactggc cgaccccttg gaggtcgaag acgacctcgg tgaggatcgc
     7621 gccgcccgct accacggccc cgaagtcgag cccccagagc gtgacaacag ggattagcga
     7681 gttgcgcagc acatgacgcc gcagcacctc gcgctcgctg agtcccttcg cgcgcgccgt
     7741 gcgaacgtag tcctcgccca tcgtgtcgag cacgttcgac cgcaggatgc gcgagtagaa
     7801 gccgatgaag agcacggcaa gcgtcgcgca cggcaggacg aggtggtacg cccagtcgag
     7861 cgggtcctcg gtcagcggca cgtagccgcc gttcgggaac cagccggcct tgaacccgag
     7921 gtagtggctc agcagcgcgc cgagccagaa gacgggcagc gagacgccga ccagcgcgag
     7981 cagggtgagc aggcgatcgg aggcccgtcc cgcgcgcatc gcgccgagca ggccgaggcc
     8041 caccccgaag gccatccaga ggatcgccgc gccgatcgcc agcgacagcg tccgcggcaa
     8101 tccccggacg atctcctcgc gcacgttgag ctgagtgaag taggagatcg cactgccgcc
     8161 gagcacctgc tccatcgtcc tgacgtactg gacgggcagc ggctcgtcga acccccactc
     8221 cgcgcggatc gcctcgacct ggctctcggt cggcgacttg ccggccatcc gcgccgcggg
     8281 atcgccgttc gggatcacgt tgaagatcag gaaggtcagc accgagacgg cgaacagcac
     8341 cacgaccatc tgggccagcc ggcgcgccac gaaggccgtc atagccgccg cgtcccccac
     8401 gccgccgacg agcgggggtc gaaggcgtcg cggacgccgt cgccgaagac gttcagcgcc
     8461 agcaccgtga gcaccagcat cgcgccgggc gcgatcgcca ggtgcggcgc ggtgatcagc
     8521 ttctcgaccc cgctgtcgat catgttcccc cacgacgggt cgggcggcac cacacccacg
     8581 cccaggaatg acagcgcggc ctcgagcagg atcgcgttgg cgaccatcag cggggcgaag
     8641 acgatgatcg tcgacgtcag gttcggcagc agctctgtcg ccatcgtgcg cagcggcccg
     8701 gccccctggg cgcgcgccgc ctcgacgaac tcgcgctcgc gcagcgccat cacctcgccg
     8761 cgaatcggcc gcgccatgta cggcacgtag acaaccgcga tcacgaggat cgagatcgcc
     8821 atcgagccgg cctcgagctg cagcggcccg acctggagcc cgccgacggc cagcgccgtt
     8881 cccagcgcca cgccgagcag gatcaccgga aacgcccaga tcacgtcgag cgtcgagcgc
     8941 gcgaccgcgt cgacccagcc gccgaagtag ccggcgacca gtcctgccat cgtcgccagc
     9001 agcgccgtca gcagcgccgc cgccaggccg atcagcagcg agttgcgcgc gccgtagagc
     9061 aggcgcacca tcacgtcgcg accgttggca tcggcgccta ggaagtactc cccgcgccac
     9121 tgcggcccga tcggcacccc ctcgagcgat acgacgtcga cctggcggcc gtcgacctcg
     9181 atcttgcccg tctcgttggt ccggtccggc tggcggtcgg cgaccgcgct cgcccacagc
     9241 ggcgccgcga gcgccgccag caccagcgcg acgaagaggg cgccgaagcc tagggcgacc
     9301 cggtcgcgcc gcaggcggcg cagggcgagc cgccacgggc tctgccccac gcgcgcgctg
     9361 tcggctattt caggcagaag ctcgagtagt cgtttccgta cagcgggtgg acgagcgagc
     9421 agttctcggc gtccatccgc tcggacagga aggtagtcag ctcctcggac ccatagggag
     9481 cgacgtagga ccgctcgatc gcccgccgct cgagctccgc ccagcggtcg gcgacctcga
     9541 ttgcgtccgg ctcctgcgac agctccgcga cgccgcggtt gatcaccgga tcgtcgacgt
     9601 agccgaagtt ggtgttgttg gtcggctgga tcgtcgtgcc gtcgagctgc tgcatgaacg
     9661 acgccgggtg cgggaagtcg gcgtaccagc tggcgagccc ggtctgcgcc ttggtgctct
     9721 ccttgccgat cgtgttgaag tagaccgccc cgtcgatcac gcgcagctcg gcgtcgaggc
     9781 cgatgtcgtt gagcagcgcc gtgtagtact cgccgatctg cggccggttg gtgtcgttgt
     9841 tggcgtaaac ggtgacctga tcgccggtcg cccccgcctg ctcgacgagc tgcttggcgc
     9901 gccggatgtc gcccgggccg ttcggatcgc cgaacggaca cgggtcgagc tcggcctgtc
     9961 cggggatgcc cggtgggatc aggttgcagg tgggcgccag ccgtccgccg aacaggcgcg
    10021 cgatcgcggt cgagtcgagc gcgacgttga ccgcctcgcg gacttgctcg tcgtcgaacg
    10081 gcggcaggct ctggttcagg aagaagtagt tggtcgagag cgtcggccac tccttgtagc
    10141 ggtcggagta gcgcgccttg atctcggccg tcaggtcggt cggcggagcg tcctgcatgt
    10201 agtcgagctc gccgtcgatg acgtcctggg cctgccgcga ggaggacttg acgatcttcg
    10261 tcgtgatcgt gtcgaggttg ccctgcggga tccccgggat gtcgaagccc tcgaccttct
    10321 ccatcacgaa ctgccggttc ggcgtcgact cggtgatcgc ataggggccg acgccgggcg
    10381 gcgggctctg cgtctggttc tcgaacggcg tgctagcggg gactaggccc gcgaaggtcg
    10441 tcgcaagggc gttgttgaag ctgccgtcgg gcgcggtcag gcggacggtg acctcaccgg
    10501 tccgatcgtt cgcctcgatg ccggcgatgt cgccgccggc ctcgcccgcc tcgacgtact
    10561 cggtcgcgcc atcgatgccc tcgaacaggt aggacccgcc cgactcgagg ttcagtacgc
    10621 gcttgatcgc gtgctcgaag tcgctcgcct tgaccggcgt gccgtcggag tacttcagcc
    10681 ccttgcggat cgtgaagctg tacgtcttgc cgtcagccga gacatcaggc agcgcctcgg
    10741 ccgcgcccgg aatcaactcc gcaccctcga cgccctcagc gcgacggtag gtcagcggcg
    10801 gcgtgtagac gagccacagc ggctcccagg agttcgaggt gtaggcgagc gccggatcga
    10861 gcgcgtcggg ctgcgagccc tgggcgatcg tgatcgcgcc accctgcttg cctccggacg
    10921 cctggtcgcc gtcggagtcg cttccgcaac cgcctccaat ggcggcggtg agggccagcg
    10981 ccaagacggt caaggagctt ctcgttgctg tcatcagacg tcctccgttg tgaagtggcc
    11041 ggcatgcgag tccgcgttcg gccagccggt atgcctgacg cggaaccctc cgacatcaca
    11101 ccccgtgggg cgccctcgct cctatcttcg gccgatacat gggctttcct ttagcccctt
    11161 gcgccaattc gtcagtttta ccgagcgggt cttagccaat tcgtacaagg catcagcgga
    11221 ggcaggcgct ggagtagtcc atccccacga ccggatggaa gcgcgcgcag ccctgcatgt
    11281 cgagccgcct cgaagccagc actcccgccg tcgccacgcc gtagggagcg ccgtaggcac
    11341 gcgtgaccac ctcctcgtcg accgccgccc agtcctgctc gaggtcagcg gcgtcgccct
    11401 cctgctcgag cagctcggca cgcgcggcca ggaccgggtc gtcgacagcg gccaggtagc
    11461 gcgacggatg cgggatcgtc ggcgagatgg ccgcgaacgc gacctgcgcg cctgctctgc
    11521 gtgtgacccg ggccgcgagg ccgatcttgc gcagcgtcgc ggcgagatag cgcgccagcc
    11581 ggcggccgcg agcgtcgccg ccggcggcga ccgccaccgc agctccgcgg gcaccggcgt
    11641 cggcgaccag ccggctcgcc tcgacgaggt cggcgtcgcc gtcgcggtcg ccgaacggac
//
LOCUS       DATABV010000224         3575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1441959, whole genome shotgun sequence.
ACCESSION   DATABV010000224 DATABV010000000
VERSION     DATABV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441959"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VHF88_10370"
     CDS             complement(<1..685)
                     /locus_tag="VHF88_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2162209.1"
                     /translation="MDLTLSPSEQAFRDELRGWLADNHPGEEPPGDEAAWQFRLAWQR
                     KLHDQGYAGLSWPEEFGGRGATLIEQAIFGEEMARAKAPSAANVLGLVMGGPVVIAHG
                     SEEQKRRYLEPILSGDEIWCQGFSEPESGSDLASLKTRAVKDGDEWVVTGQKVWTTFA
                     HHAKWCMLVARSDPDAPKHQGLTYFLMDMEQEAVQVRPLRQITGEEEFNELFIEEARI
                     PDENVVGGVG"
     gene            complement(729..1505)
                     /locus_tag="VHF88_10375"
     CDS             complement(729..1505)
                     /locus_tag="VHF88_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2162210.1"
                     /translation="MRRSAAVLAAIAVLAAAGPAAAAPDLERWRPGLEAARAYAAQRI
                     GTISFAVRTPRRLYRSDAQRVYRSASVVKAMLMVAYLNHESVRGRPLNAADHALLNPM
                     VRWSDNLAATRVRELVGNGALERLADRVGMRRFATMPSWGDTQITAAGQSKLFLRIDR
                     YVVRRHRDTALRLLGAIVPEQRWGIARVPHPGWRLHFKGGWGDYGEVDHQIALLRRGK
                     RRIAVAILSTAFPSHEYGKRTLEGIARRLLHGLGPRSNPR"
     gene            1626..2879
                     /gene="xseA"
                     /locus_tag="VHF88_10380"
     CDS             1626..2879
                     /gene="xseA"
                     /locus_tag="VHF88_10380"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX2162211.1"
                     /translation="MATPEQQRIPPVGIEGSALKGPFAVGEYAEALRGQLRGFARVQV
                     FGEVSNLGIRAKAVYFELRDERGALPCSMWRDDYDKAAVELENGAQVVLAGGCDYYPG
                     SATSSPSFSFRATGLKLAGEGELLAQIERLRRRLHGEGLFEPQQRLPRPALPRSIGVV
                     TGEGGKARDDVLAGLRRRGWAGRLVWGFAPVQDRRAAPAISRAIQDLAAVAEVEVIVV
                     ARGGGSVMDLMAFSDETLCRTVALLPVPVIASVGHHTDRTLIDDVAAVSCSTPTHAAE
                     SAVRVDVNEARANALATARRLDSCGRRAVVSRARHLAALSRAPNQHIARQRGWLHQKT
                     RELRAASNRQLAVRAERTDRHRLVIDRKAEAAKQEPEQMLKRGYALVEDGRGELVTSA
                     GTARRLRDLSVRFHDASVRAKVDDG"
     gene            complement(2904..3446)
                     /locus_tag="VHF88_10385"
     CDS             complement(2904..3446)
                     /locus_tag="VHF88_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2162212.1"
                     /translation="MPFAALALVLVAAAIGVAALSGGGDDGRETTSERAGNGAAANGA
                     RSDRKGSGKADRKIAQGGESPPKARPAPQPEPEPEQEPEAQSEPVTGDPAALNERGYE
                     LMQAGDYEGAIPLLEQAVAAYPEGSQDLTYAYALYNLGRSLRLAGRADEAVPILERRL
                     QIPNQTETVQQELDAARAAS"
BASE COUNT          466 a         1352 c         1264 g          493 t
ORIGIN      
        1 tgccgacccc gccgacgacg ttctcgtcgg gaatccgcgc ctcctcgatg aagagctcgt
       61 tgaactcctc ctcgccggtg atctggcgca gcggccgcac ctggacggcc tcctgctcca
      121 tgtccatcag gaagtaggtc aaaccctggt gcttcggcgc gtcgggatcg ctgcgcgcca
      181 cgagcatgca ccacttggcg tggtgggcga acgtcgtcca gaccttctga ccggtgacca
      241 cccactcgtc gccgtccttg accgcgcgcg tcttcagcga cgccaggtcg gagcccgact
      301 ccggctcgga gaagccctga caccagatct cgtcgccaga caggatcggc tcgaggtagc
      361 gccgcttctg ctcctcgctg ccgtgggcga tcacgaccgg cccgcccatc acgaggccga
      421 gcacgttggc ggccgacggc gccttggcgc gcgccatctc ctcgccgaag atcgcctgct
      481 cgatcagcgt cgcgccgcgg ccgccgaact cctccggcca ggacaggccg gcgtagccct
      541 ggtcgtggag cttgcgctgc caggcgagcc ggaactgcca tgccgcctcg tcgccgggcg
      601 gctcctcgcc cgggtggttg tcggcgagcc agccgcgcag ctcgtcccga aatgcctgct
      661 cgctgggcga gagggtcagg tccacgacgc gaccctagcg cagattggcc gggcggccag
      721 gaagcggatc agcgcgggtt cgagcgcggg ccgaggccgt gcagcagccg ccgcgcgatg
      781 ccctcgagcg tccgcttgcc gtactcgtgg ctcgggaacg cggtgctgag gatcgcgacc
      841 gcgatccgcc gcttgccgcg ccgcagcagc gcgatctggt ggtcgacctc gccgtagtcg
      901 ccccaaccgc ccttgaagtg gaggcgccag ccgggatgcg gcacgcgggc gatcccccag
      961 cgctgttcgg gcacgatcgc gcccaggagg cgcagggccg tgtcgcggtg gcggcgcacc
     1021 acatagcggt cgatgcgcag gaacagcttg ctctgaccgg ccgccgtgat ctgggtgtca
     1081 ccccagctcg gcatggtcgc gaagcgccgc atcccgacgc ggtcggcgag gcgctcgagc
     1141 gccccgttgc caacgagctc gcgcacgcgc gtcgccgcaa ggttgtccga ccagcgaacc
     1201 atcgggttca acagcgcgtg gtcggcagcg ttcagcggac gtccccgcac gctctcgtgg
     1261 ttgaggtagg cgaccatcag catcgccttg acgacgctgg ccgagcgata gacgcgctgg
     1321 gcgtcggagc ggtagagcct gcgcggcgta cgaaccgcaa acgagatcgt gccgatccgc
     1381 tgcgccgcat aagcgcgggc cgcctcgaga ccggggcgcc agcgctccag gtccggagcc
     1441 gccgcagctg ggccggcggc tgcgagaacc gcgatcgctg ccaggacggc tgccgagcgc
     1501 ctcatgcgcg gacggtaccc gtgccgaccg gccgctcgtc gcggcgggcg acagtgggcg
     1561 gattccggcg acattgcggc cggaatccgc ccagcgcgcc gcggctgctg cgaacatcga
     1621 tggccatggc cacgccggag cagcagcgaa tcccgcccgt cgggatcgag ggcagcgcgc
     1681 tcaagggacc gttcgcggtc ggcgagtacg cggaggcgct gcgcggccag ctgcgcggct
     1741 tcgcgcgcgt gcaggtcttc ggcgaggtct cgaacctggg gatccgcgcc aaggccgtct
     1801 acttcgagct gcgcgacgag cgcggcgcgc tgccctgctc gatgtggcgc gacgactacg
     1861 acaaggcggc ggtcgagcta gagaacggcg cccaggtcgt gctcgccggc ggctgcgact
     1921 actacccggg cagcgccacc tcgtcgccgt cgttctcctt ccgcgcgacc gggctcaagc
     1981 tcgcgggcga gggcgagctg ctcgctcaga tcgagcgact gcgccgccgg ctgcacggcg
     2041 agggcctgtt cgagccccag cagcggctgc cgcgccccgc cctgccgcgc tcgatcggcg
     2101 tcgtcaccgg cgagggcggc aaggcacgcg acgacgtgct cgccggcctg cgccgtcgcg
     2161 gctgggccgg ccgcctggtc tggggcttcg cccccgttca ggatcgccgc gccgcgccgg
     2221 cgatctcacg cgcgatccag gatctcgccg cggttgccga ggtcgaggtg atcgtcgtcg
     2281 cgcgcggcgg cggctcggtg atggacctga tggccttttc cgacgagacg ctctgccgga
     2341 ccgtcgccct gctgccggtc ccggtgatcg cctcggtcgg ccaccacacc gatcgcacgc
     2401 tgatcgacga cgtcgcggcc gtctcgtgct cgacgccgac ccacgcggcg gagtcggccg
     2461 tccgggtcga cgtgaacgag gcccgcgcca acgcactcgc cacggcgcgt cgtctcgaca
     2521 gctgcggccg ccgcgccgtc gtcagccgcg cccgccacct cgcggcgctc tcacgtgccc
     2581 cgaaccagca catcgctcgc cagcgcggct ggctccatca gaagacccgc gagctgcgcg
     2641 ccgcgagcaa ccgccagctc gccgtccgcg ccgagcgtac cgaccgccac cggctcgtga
     2701 tcgaccgcaa ggccgaagcc gccaagcagg agccggagca gatgctgaag cggggctacg
     2761 ccttggtcga ggacggtcgc ggcgagctgg tcacgagcgc agggaccgcg cgcaggctgc
     2821 gcgacttgag cgtgcgcttc cacgacgcca gcgtccgcgc caaggtcgac gacggctgat
     2881 ctggcctagc gacgtgcggc gcctcagctg gccgcgcggg cggcgtcgag ctcctgctgc
     2941 accgtctcgg tctggttcgg tatctgcagc cgccgctcga ggatgggcac cgcctcgtcg
     3001 gcccgtcccg cgagccgcag cgagcgaccg aggttgtaga gcgcgtaggc gtaggtgagg
     3061 tcctgcgatc cctccggata ggccgcgaca gcctgctcga gcagggggat cgccccctcg
     3121 taatcccccg cctgcatcag ctcgtagcca cgctcgttga gcgcggcggg atcaccggtc
     3181 accggctcgg actgcgcctc gggctcctgc tccggctcgg gctccggctg cggggcaggc
     3241 cgtgccttcg gcggcgactc gccaccttgc gcaatcttgc gatccgcctt ccccgatccc
     3301 ttccggtcgg agcgggcccc gttcgctgcc gcgccgttgc ccgcccgctc gctcgtggtc
     3361 tcgcggccgt cgtcaccgcc gccgctcagc gcggcgacgc cgatcgccgc cgccaccagc
     3421 accagcgcca gcgccgcgaa cggcacccaa gcgcgccgac gcgctggtcg aggcccggcg
     3481 gtcgaccgac ccgcgtcgtc gtcgcgtcgc ggtggctcgg caggcggcgc ggcccacgca
     3541 tcgcccggcg cgccctgcga cccgaccgcc gcggc
//
LOCUS       DATABV010000225         5443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2726185, whole genome shotgun sequence.
ACCESSION   DATABV010000225 DATABV010000000
VERSION     DATABV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726185"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VHF88_10390"
     CDS             <1..635
                     /locus_tag="VHF88_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162213.1"
                     /translation="PPPRLLPSPALAVACIALFVALGGVSYGLATGSIDTREIRDNTI
                     RSKDVRNNSIYTRDIRNNDVRDIDIRNGTIKGRDVARGTLTGDNINMRKLGQVPDAAA
                     LGGRPAADYALASAPEPVLFVAGVSPAAPGDLAPGFWKHDATVYLQGSVSGSGSLFTL
                     PEGYRPAGNAHFRVPAALPAGETTVTVSASGVVSSADPQPALDGISFRAP"
     gene            complement(653..2311)
                     /locus_tag="VHF88_10395"
     CDS             complement(653..2311)
                     /locus_tag="VHF88_10395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX2162214.1"
                     /translation="MAGKLRVLPLGGLGEIGKNMTVLEYEDRIVVVDTGLRFPAPDQL
                     GIDLVLPDFAYLRDRADDIEAIFITHGHEDHIGALPYVLRELDIAPRIYGGPLTMAMA
                     RSKLDEHKLDAEVTYVRPGDVKSAGPFDVEPVKLAHSIPDMFAYALTCDLGTTLITGD
                     YKFDQTPVDGVPADIARLAELGSDGVLLLCGDSTNADRGGWSLSESLVGPKLEETFAR
                     CEGRIVVTSFASNIHRVQQVVDAAEVLGRKVALVGRSMRKNVNIGSQLGHIHIPEGML
                     VEPREIEDFPDHKLIILSTGSQGEPLSALRRMAHGDHQHVKLHQGDTVVFSATPVPGN
                     ERAVNDTIDRLFHIGADVVTPREAPIHASGHGYAEELKLMLNLTNPRYVLPVHGDYKR
                     IKLHGELAMAVGIEPDAIFSGDNGIPLEIDAGGAAFGEPEQAGMIFVDGVDVGDVEDV
                     ALRDRRMLSADGIFIVVATVSEQSGRSVAEPEIIFRGVPFVDEQDQFMVDLRGAVIGS
                     LARSAQDDIREIDLIQTRLHDDLAAFVYKKRKRRPMVLPVVVEV"
     gene            complement(2313..3233)
                     /gene="dapA"
                     /locus_tag="VHF88_10400"
     CDS             complement(2313..3233)
                     /gene="dapA"
                     /locus_tag="VHF88_10400"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX2162215.1"
                     /translation="MTVSGILTAMVTPFDSDGALAEESTARLVRHLLANGSDGLVLTG
                     TTGEASTLSDDEKARIWDIGIEVSRAERPDAPVIAGTGSNDTRHAVELTARAAAAGVD
                     AALVVAPYYNRPNRRGLLAHFQAVAGSTDLPIVAYNIPARTGVDMPNDLLAELAQIEN
                     VVAVKQARADDLAPIDGLDLLAGNDDMLGAVLDLGGAGGILVASHLVAAEMRRMVDEP
                     TERHAIEERLAPLYDALAVAPACVSVKAALNLLGHDVGGVRLPMVEASADELDKIRSG
                     LEKVGGLAPPGPPGAPGRSAGNQSAGRERV"
     gene            3326..3745
                     /locus_tag="VHF88_10405"
     CDS             3326..3745
                     /locus_tag="VHF88_10405"
                     /inference="COORDINATES: protein motif:HMM:NF022698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HEX2162216.1"
                     /translation="MTERDQSHRTTVQKLGVKEGERIEVIGDVGPGLRDGVKAAIGRG
                     LVRAGELDGAIVLVESLDEAKETLVRYRARIKDSGYLWVITRKRGHDHYLDQMKIVPV
                     AKRVGMIDNKTCSIDDERSGIRFVVPRALRKDAADAA"
     gene            complement(3832..4488)
                     /locus_tag="VHF88_10410"
     CDS             complement(3832..4488)
                     /locus_tag="VHF88_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2162217.1"
                     /translation="MIRVAVSGAAGRMGEEVCRAVEGADDMELTGRADPALGTELGAV
                     LGDAEVVVDFTRPDTALDNARQCLEAGVHCVMGTTGADFGELSGVGSANLFVAPNFAI
                     GAVLMMEAAKLIAPHMPECEIVELHHDRKLDAPSGTAARTAELIAGAGGNVHDPIHSI
                     RLPGLVAHQEVVFGGLGQTLSVRHDSISRESFMPGVLLAVRRVGQLDHSPAFGLETIL
                     "
     gene            complement(4485..>5443)
                     /locus_tag="VHF88_10415"
     CDS             complement(4485..>5443)
                     /locus_tag="VHF88_10415"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2162218.1"
                     /translation="VLHPTYPDIDSERDVVIEEIAMYEDEPQDKVHDVLSTAVFGDHP
                     LGRPIIGRADVVSSVPVPEIAAFHDERYVGGNLVLAAAGNVDHERLVELAREKLDCST
                     APGVDGPAEAAPAEITPRRCFHAKETEQYHLCVGGPGIARDDERRFALRILDTVLGGS
                     TSSRLFQEVREKRGLAYSVYSYASQFVDSGQVGIYVGTRPDNVAEAMGIIARELERIA
                     VDGVSADELERARENVKGRTTLSMESTLARMNRLGSSVLMGVPVLTLDEILAALDAVT
                     LDDLAALAAELYAPERLSAAGVGASEDSFRSALAPLSEALAA"
BASE COUNT          806 a         1919 c         1901 g          817 t
ORIGIN      
        1 cccccccccc ccgcttgctt ccctctcccg cattggccgt cgcctgcatc gccctgttcg
       61 tcgcgctggg cggagtttcc tatggcctcg ccacgggatc gatcgacacc cgcgagatcc
      121 gtgacaacac cattcgcagc aaggacgtcc gcaacaacag catctacacg cgcgacatcc
      181 gcaacaacga cgttcgcgac atcgacatcc gcaacggcac gatcaagggc cgcgacgtcg
      241 ccagagggac cttgaccggc gacaacatca acatgcggaa gctgggccag gtgcccgacg
      301 ccgcggcgct gggcgggcga ccggccgccg actacgcgct ggccagcgcc ccggagccgg
      361 tcctgttcgt ggcaggcgtc tcgccggcag ccccgggcga tctcgcgccc ggcttctgga
      421 agcacgatgc gacggtctac ctgcagggct cggtcagcgg cagcggcagc ctcttcacgc
      481 tgcccgaggg ctaccgaccg gctgggaacg cccacttccg tgtcccggcc gcgcttccgg
      541 ccggcgagac gacggtgacg gtcagcgcgt cgggcgtcgt ctcgagcgcg gatccccagc
      601 cggcgctcga cggaatctcg ttccgggccc cgtagcgggc gcggcgcgag gtctatacct
      661 cgacgacgac cggcaggacc atcgggcggc gcttgcgctt cttgtagacg aaggccgcca
      721 ggtcgtcgtg caggcgcgtc tggatcaggt cgatctcgcg gatgtcgtcc tgggcgctgc
      781 gcgccagcga cccgatgact gcgccgcgca ggtcgaccat gaactggtcc tgctcgtcga
      841 cgaacggcac cccgcggaag atgatctcgg gctcggcgac cgagcggccg ctctgctcgg
      901 acacggtcgc gaccacgatg aagatgccgt ccgcggacag catccgccga tcgcgcagcg
      961 cgacgtcttc gacgtcgcca acgtcgacgc cgtcgacgaa gatcatcccg gcctgctcgg
     1021 gctcgccgaa tgccgcgccg ccggcgtcga tctccaacgg gatcccgttg tcgccgctga
     1081 agatcgcatc cggctcgatc ccgaccgcca ttgccagctc gccgtgcagc ttgatccgct
     1141 tgtagtcgcc gtggaccggc agcacgtagc gcgggttcgt caggttgagc atcagcttca
     1201 gctcctcggc gtagccgtgc cccgaggcgt ggatcggcgc ctcgcgtggc gtcacgacgt
     1261 cggccccgat gtggaagagg cggtcgatcg tgtcgttgac cgcgcgctcg ttcccgggga
     1321 ccggcgtcgc cgagaagacg acggtgtcgc cctggtgcag cttgacgtgc tgatggtcgc
     1381 cgtgggccat ccggcgcagg gccgacagcg gctccccctg gctgccggtc gacaggatga
     1441 tcagcttgtg atcgggaaag tcctcgatct cacgcggctc gaccagcatg ccctccggga
     1501 tgtggatgtg gccgagctgg ctgccgatgt tgacgttctt gcgcatcgat cgcccgacca
     1561 gcgccacctt gcgccccagc acctcggcgg cgtccacgac ctgctgcacg cggtggatgt
     1621 tggaggcgaa cgacgtgacg acgatccggc cctcacagcg cgcgaacgtc tcctccagct
     1681 tcggcccgac gagcgactcc gacagcgacc agccgccgcg gtcggcgttg gtcgagtcgc
     1741 cgcagagcag cagcaccccg tcgctgccca gctcggcgag ccgcgcgatg tcggccggga
     1801 cgccgtccac cggtgtctgg tcgaacttgt agtcgccggt tatcaaggtc gtgccgaggt
     1861 cgcaggtcag cgcgtaggcg aacatgtccg ggatcgagtg cgccagcttg accggctcga
     1921 cgtcgaaggg gccggcggac ttgacgtcgc cgggccggac ataggtcacc tcggcgtcga
     1981 gcttgtgctc gtcgagcttg gagcgcgcca tcgccatcgt cagcgggccg ccgtagatgc
     2041 gcggcgcgat gtcgagctcg cgcagcacgt agggcagcgc gccgatgtgg tcctcgtgcc
     2101 cgtgcgtgat gaagatcgcc tcgatgtcgt ccgcgcggtc gcgcaggtag gcgaagtccg
     2161 gcagcacgag atcgatcccg agttggtcgg gtgccgggaa gcgcaggccg gtgtcgacca
     2221 cgacgatccg gtcctcgtat tcgaggacgg tcatgttctt gcctatctcc cccagcccgc
     2281 cgagcggaag gacccgcagc ttgcccgcca agctagaccc gctcccggcc cgctgactga
     2341 tttccggccg accggccagg cgccccgggg ggcccggggg gcgcgagccc cccgaccttc
     2401 tccagcccgc tgcggatctt gtctagctcg tcggcggagg cctcgaccat cggcagccgg
     2461 acaccgccaa cgtcgtggcc gagcaagttg agcgccgcct tgaccgatac gcacgccggc
     2521 gcgacggcca gcgcgtcata gagcggcgcg aggcgctcct cgatcgcatg acgctcggtc
     2581 ggctcgtcga ccatccggcg catctcggcg gcgaccaggt gcgacgcgac gaggatgccg
     2641 ccggcccccc caaggtcgag caccgcaccg agcatgtcgt cgttgccggc gagcaggtcg
     2701 agaccgtcga tcggcgcgag gtcgtcggcg cgcgcctgct ttaccgccac gacgttttcg
     2761 atctgcgcga gctcggcgag caggtcgttc ggcatgtcga cgccggtgcg cgccgggatg
     2821 ttgtaggcga cgatcggcag gtcggtcgag cccgcgaccg cctggaagtg cgccagcagg
     2881 ccgcgccggt tcgggcggtt gtagtacggc gcgaccacca gcgccgcgtc gacgccggca
     2941 gccgccgcgc gggcggtgag ctcgaccgcg tggcgggtgt cgttcgaccc cgttcccgcg
     3001 atcaccggcg catcgggccg ctcggcgcgc gagacttcga tgccgatgtc ccagatccgc
     3061 gccttctcgt cgtcgctcag cgtcgacgcc tcacccgtcg tgccggtcag caccaggcca
     3121 tcggacccgt tcgcgagcag gtggcggacg agccgcgcgg tcgattcttc ggcaagcgcg
     3181 ccgtctgagt cgaacggcgt gaccatcgcc gtgaggatgc ccgagaccgt cacgctggcc
     3241 acattaccgt gcctgcctgg ccacggcatg gcgcttgcag actggtcaga ccgtctgtcg
     3301 gaaatgtcac aatggacggt cggagatgac tgagcgcgac caaagccacc gcaccaccgt
     3361 ccagaagctc ggcgtcaagg agggcgagcg gatcgaggtg atcggagacg tcggccccgg
     3421 cctacgcgac ggcgtcaagg ccgcgatcgg gcgcggcctc gtgcgtgccg gcgagctcga
     3481 cggagcgatc gtgctggtcg agagcctcga cgaggccaag gagacgctcg ttcgctatcg
     3541 cgcgcggatc aaggactccg gctatctctg ggtgatcacg cgcaagcgcg ggcacgacca
     3601 ctatctcgac cagatgaaga tcgtcccggt ggcgaagcga gtcgggatga tcgacaacaa
     3661 gacgtgctcg atcgacgacg agcgctccgg catccgcttc gtcgtgcccc gcgcgctgcg
     3721 caaggacgcg gccgacgcgg cctagagaac agccggcgga ggagcgcagc cggaccggtc
     3781 ccccggctac gcggcgcagc tagcgggagc gaggtccggc gagcgccgca gctacagaat
     3841 cgtctcgagg ccgaacgccg gcgagtggtc cagttggccg acgcggcgga cggcgagcag
     3901 gacgccgggc atgaacgatt cgcgcgagat cgagtcatgg cgcacgctca gcgtctgacc
     3961 caatccaccg aagacgacct cctggtgggc gacgaggccg ggcaggcgaa tcgagtggat
     4021 cgggtcgtgg acgttgccgc ccgcgcctgc gatcagctcg gccgtgcgtg ccgcggtgcc
     4081 cgacggcgcg tcgagcttgc ggtcgtggtg cagctcgacg atctcgcact ccggcatgtg
     4141 cggggcgatc agctttgccg cctccatcat caggacggcg ccgatcgcga agttcggcgc
     4201 gacgaagagg ttcgcgctgc cgacgccgct cagctcgccg aagtcggcgc ccgtcgtgcc
     4261 catcacgcag tggacgccgg cctcgaggca ctggcgcgcg ttgtcgaggg ccgtgtcggg
     4321 ccgggtgaag tcgacgacga cttcggcatc gcccagcacc gccccgagct cggttcccag
     4381 cgccgggtca gcgcggcccg tcagctccat gtcgtccgcg ccctcgacag cccggcagac
     4441 ctcctccccc atcctgccgg cggcgccgga gacggcgacg cggatcacgc cgcgagtgcc
     4501 tcgctgagcg gggccagggc gctgcggaag gagtcctcgc tggctccgac gccggccgcc
     4561 gacaggcgct ccggcgcgta gagctcggcc gcgagtgccg ccaggtcgtc gagcgtcacc
     4621 gcgtcgagcg cggccaggat ctcgtcgagc gtcaggacgg ggacgcccat cagcaccgag
     4681 ctgccgagcc ggttcatccg tgcgagcgtc gactccatcg agagcgtcgt gcggcccttg
     4741 acgttctcgc gtgcccgctc gagctcgtcg gcgcttacgc cgtcgacggc gatgcgctcg
     4801 agctcgcggg cgatgatccc catcgcctcg gcgacgttgt ccggtcgcgt gccgacgtag
     4861 atgccgacct ggccgctgtc gacgaactgg ctcgcatacg agtagacgga gtaggcgagg
     4921 ccgcgcttct cgcggacctc ctggaacagg cgcgacgagg tcgagccgcc gaggaccgtg
     4981 tcgaggatcc gcagcgcgaa gcgccgctcg tcgtcgcgcg cgatgcctgg cccgccgacg
     5041 cagaggtggt actgctcggt ctcctttgcg tggaagcagc ggcgcggcgt gatctccgcc
     5101 ggcgccgcct cggccggacc gtcgacgccc ggtgcggtgc tgcaatcgag cttctcgcgc
     5161 gcaagctcga cgagtcgctc atggtcgacg ttgccggcgg ctgctaggac caggttgccg
     5221 ccgacatagc gctcgtcgtg gaacgccgcg atctcgggca ccggcaccga cgagaccacg
     5281 tcggcgcgtc cgatgatcgg gcgcccgagc ggatggtcgc cgaagaccgc cgtcgagagc
     5341 acgtcgtgaa ccttgtcctg cggctcgtcc tcgtacatcg cgatctcctc gatcacgacg
     5401 tcgcgctcgg agtcgatgtc cgggtaggtc gggtgcagca cca
//
LOCUS       DATABV010000226         8708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_2003635, whole genome shotgun sequence.
ACCESSION   DATABV010000226 DATABV010000000
VERSION     DATABV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003635"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VHF88_10420"
     CDS             complement(<1..466)
                     /locus_tag="VHF88_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162219.1"
                     /translation="MARPAGDRAVVRALAAYVPAGRVDSGELRRHWDPQAPDSAGPPL
                     AVPDFDEDVVTMAVEAAGRALAAAGVAAEDVGAVVLATCSAPYGEPSAAGLVARALRV
                     PPGCETTTLGGSTRGGLQALRCAADAVTAGRAERVLAIAAEDRRGRAGTALER"
     gene            complement(459..1613)
                     /locus_tag="VHF88_10425"
     CDS             complement(459..1613)
                     /locus_tag="VHF88_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2162220.1"
                     /translation="MAVVGIPEKKALLEAVADFSRQEIAPRAAAYDQAEQLPRDLLAA
                     MAELNIFGGVVDPEYGGLGLDYVTFAELVTEVSKACQVLGTLVSMPSGLVGASIASFG
                     TEEQKQRWLAPLARGETFGAAAVTEPNSGSDVAGMQTTYVRDGDEFVINGAKAWISNL
                     DVASFFVTFASFDRSLRHRGVTAFIVPRDTPGLTLTPYKDKLGFRPLSTGEVSLADVR
                     LGADALLGEEGGGFPVAMTAVERGRLGVAARSVGVIHACLDDSVAYALERNAFGHPIA
                     DFQLVQSKITDMTVGLETATLLVGRCAEALDRGERARKLTSMAKMYASDVAARSASDA
                     VQIHGAYGASSEYRVARMYRDAKVLQIVEGSNDLDRALIGELALGLRSGG"
     gene            complement(1642..2505)
                     /locus_tag="VHF88_10430"
     CDS             complement(1642..2505)
                     /locus_tag="VHF88_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162221.1"
                     /translation="MSAAGGAPSPPGQTVVASSARGRVVAVDSAYHVAEANRAADVIV
                     AASYCGVLPARFVAERAPRAAIGVDCAIGKDGAGIAGLWYLEALGIPAAAAAVMSVHL
                     GDGVDVWRSGRLSRVNAPAESVGVTAGMAVAEACELLLERDPVARPADEISHRTVVET
                     APDGAQVVCTDSIAFGLPEDRDRNVLCTAGHTGRSAVPYLLDVRPRGFVCSDGGRGRD
                     DAGIAGLAIVEREGLAGAAVDALTARMGDGLSTYRDGVVSACNAPARERGVAIGQTAR
                     EAAGRLLALQP"
     gene            complement(2502..3278)
                     /locus_tag="VHF88_10435"
     CDS             complement(2502..3278)
                     /locus_tag="VHF88_10435"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX2162222.1"
                     /translation="MSVLISGSVAARARRNYKPRKTAMLLAQRIVSEITERGLEPGVM
                     LPPEQEMLNEYGVARGTLREALRYLESQGVLTIKPGPRGGPVVTSPDPRYLASTLALL
                     LQVTGAPYRTVVEARQIVEPAMAARAAVLVSEEQLDQLRASVERMGEVLDSLPDFLEE
                     NRAFHDLIAHVSGNQLFEHLLASLGWITDGTALGVDYPKRSRQHVLEVHGSILEAIEQ
                     RDAALAESRMREHIDAYVGYLQRHYKQLLDRELRWENVWS"
     gene            complement(3275..4930)
                     /locus_tag="VHF88_10440"
     CDS             complement(3275..4930)
                     /locus_tag="VHF88_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2162223.1"
                     /translation="MNVYEEYAPTFADRACWTVPWVLRERARTHGDSIYLDAPGAGER
                     HSYAEVLARAERIASGLIAAGGEPGDRLLVMAPNSPQVVFAWFGSACAGMAEVPINTA
                     YRGSFLEHQVRTTAPRFAVIDAELAERFVESAEACATIERFYLVGDAERQATATQALA
                     AKGWAVEPFAALESTTPAPLPEVRPHDLASVFFTSGTTGLSKGVMMPHAQMVFFADEC
                     VSLTRLTDADAYLSVGPLFHGNAQFLAALPALIAGARFVLRERFSASRWIDWVRDSGA
                     TVTNFVGVMMDFVWKQPARPDDGENELRCVFAAPTASSILDGFRERFGIDAFVEVFGL
                     TETSMPIMTPYGVERPAGACGKVNADWFDVRLVDPETDEEVAVGQTGELVVRPRQPWT
                     TCIGYYGMADKTAEAFRNLWFHTGDGLRRDADGWFYFVDRLKDAIRRRGENISSYEIE
                     QAVLDHPGVIECAAIAVAADVDAGEDEVMLFTVCTDGASREDVHDWCVRRLPAFALPR
                     YVRLVDDLPKTPSGKVRKAELRRLALERGLAGDELPVPAGGRA"
     gene            complement(4927..5742)
                     /locus_tag="VHF88_10445"
     CDS             complement(4927..5742)
                     /locus_tag="VHF88_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2162224.1"
                     /translation="MAAAERGVNAEGGPVVRVERRAEVAWVTLDVPERMNAYDLATAR
                     ELIAALDKTADAGVVAITGTGRAFCAGGYLANLAEASETELRSLFQASLDLYDTIRRH
                     PRPVIAAVNGAAFGGGNELVVACDLAIAAESARFGQTGPRVGSAPVLGGTNMLALIAG
                     EKRAKEISLLCRPYDARQAHELGMVNAVVADAELEAEVGRWADELLALSPRYLEIAKI
                     SSNVWWDACRGSFLSGLGMLAQAAGSEDMREGAQAFLEKRKPRFPGRAPREDA"
     gene            5762..6895
                     /locus_tag="VHF88_10450"
     CDS             5762..6895
                     /locus_tag="VHF88_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2162225.1"
                     /translation="MPIARLEPAYLELQREARALAESVEPFAAEADESSAVDARVLDR
                     LRASGLCGVAVPAGWGGRSESVDPLAVAVVREALMGSSSQLDSLFALQGIGSYAIARG
                     GSDAQRDRWLPDVAGGAVLAGLALTERDAGSDLKAVATTLQPTADGLMLRGEKSFISN
                     AGAAGFYTVLAREDAGLSLVLVEADAPGVSVTRTQEIIAPHVLGDLHFDGVMLPPSAR
                     IGAPGGGLELVLATLGVFRASVAAAAVGLAQAALEEATRHAASRSQFGRPLARHGPVA
                     ALLADSWTEIEMARLLTYRAAELARDDPQGALNWSSMAKLAATEMASRVVDRSVQAMG
                     RWGLSRDRKIERLYRQARPMRIYEGASEVLRLGIARTLVDTLE"
     gene            6892..7644
                     /locus_tag="VHF88_10455"
     CDS             6892..7644
                     /locus_tag="VHF88_10455"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2162226.1"
                     /translation="MSGYGLDGRVAIVTGASRGLGLAAARALLAEGVRVVAAARSADA
                     LDTLAAEHPGQVEAVACDMRDVDAVAALPGIALDRCGGLDVLVNNAGIAPAGAFDEQR
                     LETWREVLDVNVLAPVALTRAAAPHLLDQGAGKVINVASTSGLLGKPTLAAYSASKGA
                     LIQFTKALAAEWAARGIQVNAIAPGAFETDAQQAVLDSPEVLARRLRKIPAGRMGQPG
                     EIGPLVCWLASNQSDFVSGATLVIDGGESSKL"
     gene            7648..8466
                     /locus_tag="VHF88_10460"
     CDS             7648..8466
                     /locus_tag="VHF88_10460"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2162227.1"
                     /translation="MVIDAHTHVWPDAIAGRALAGSVRDLERFGDGRVDSLIATMDAA
                     GIDRSVCLGVANSGDRVEAANRFAGSLDRDRLIGFGSIHPDLSAAENVASLRRHGLVG
                     AKSHPLFQSYSLDDPRLLEILDALRGEFAVIVHVGPAGADSGARCTPAMIRDIARQLP
                     GLALIACHLGGYRVLDEAEDSVVGLPGVYLDTSWPPSIATLDRNRVRAAIERHGPERV
                     LFASDWPMADPGAELAAVEALGLSDADTRAILGGNLVRLLSELPASSAGGPATD"
     gene            complement(8440..>8708)
                     /locus_tag="VHF88_10465"
     CDS             complement(8440..>8708)
                     /locus_tag="VHF88_10465"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX2162228.1"
                     /translation="RIGGRARGWDDLLHLVAAGQAIAIVPASIAAALGPTTAGVRFTT
                     VEDIEPSPLVLAWRPADETPAVRELAELARSLTARASVGGGPAR"
BASE COUNT         1163 a         3247 c         3139 g         1159 t
ORIGIN      
        1 ggcgctcgag cgccgtcccg gctctgccgc ggcggtcctc cgcggcgatt gccagcacgc
       61 gctcggcacg cccggccgtg acggcgtcag cagcgcagcg cagggcctgc agcccgccgc
      121 gcgtcgaccc gccgagcgtc gtcgtctcgc agcctggtgg gacgcgcagc gcacgcgcca
      181 ccagcccggc agccgacggc tcgccgtagg gagcggagca ggtggccagc acgacggcgc
      241 cgacgtcctc ggccgccacg cccgcggcgg ccagcgctcg accggcggcc tcgacggcca
      301 tcgtcacgac gtcctcgtcg aagtcgggca ccgcgagcgg cggtcccgcg gagtccggcg
      361 cctgaggatc ccagtggcgc cgcagctcgc cgctgtcgac gcgaccggcc ggcacgtatg
      421 ccgccagggc gcggacgacc gcgcgatcac cggccggcct agccacccga gcgcagcccc
      481 agcgccagct ccccgatcag cgcacggtcc aggtcgttgc tgccctcgac gatctgcagc
      541 accttggcgt cgcggtacat gcgcgcgacg cggtactcgc tcgacgcgcc gtaggcgccg
      601 tggatctgca cggcgtcgga ggccgaacgc gccgccacgt cgctggcgta catcttcgcc
      661 atgctggtca gcttgcgcgc ccgctcgccg cggtcgagcg cctcggcaca gcggccgacg
      721 agcagcgtgg ccgtctcgag gccgaccgtc atgtcggtga tcttcgactg gacaagctgg
      781 aagtcggcga tcgggtggcc gaacgcgttc cgctcgagcg cgtaggcgac cgagtcgtcg
      841 aggcaggcgt ggatcacgcc gaccgagcgg gcggccacgc ccagccgtcc acgctcgacg
      901 gcggtcatcg cgaccgggaa gccgccgccc tcctcgccga gcagcgcgtc ggcgccgagg
      961 cggacgtcgg ctagcgagac ctcgccggtc gacagtggcc tgaagccgag cttgtccttg
     1021 tacggcgtga gtgtgagccc cggcgtgtcg cgcgggacga tgaaggcggt cacgccgcgg
     1081 tggcgcagcg agcgatcgaa ggaggcgaag gtgacgaaga agctcgcgac gtcgaggttc
     1141 gagatccagg ccttggcgcc gttgatcacg aactcgtcgc cgtcgcggac gtaggtcgtc
     1201 tgcatccccg cgacgtcgct gccggagttc ggctcggtga ccgccgcggc accgaacgtc
     1261 tcgccgcggg ccagcggcgc gagccagcgc tgcttctgct cctcggtgcc gaacgaggcg
     1321 atgctcgccc cgacgagccc ggacggcatc gagacgagcg tcccgagcac ctgacaggcc
     1381 ttgctgacct cggtgaccag ctcggcgaag gtgacgtagt cgagccccag ccctccgtac
     1441 tcagggtcga cgacgccgcc gaagatgttc agctcggcca tcgccgcgag caggtcgcgc
     1501 ggcagctgct cagcttggtc gtaggccgcg gcgcgcggag cgatctcctg gcgggagaag
     1561 tcggcgaccg cctccagcag cgccttcttc tccgggattc caacgaccgc catatctgat
     1621 cataataata cctaatcgca gctatggctg caaggcgagc aggcgaccgg cggcctcacg
     1681 cgccgtctgc ccgatggcga caccgcgctc gcgagccggc gcgttgcagg cgctgaccac
     1741 gccatcgcgg taggtactca ggccgtcgcc catccgggcg gtcagcgcgt cgactgccgc
     1801 cccggcaagg ccctcgcgct cgacgatcgc gagcccggcg atgcccgcgt cgtcgcggcc
     1861 gcggccgccg tcggagcaga cgaagccgcg cggacggacg tcgagcaggt agggcaccgc
     1921 gctgcggccg gtatggccgg cggtgcagag cacgttgcga tcgcggtcct cgggcaggcc
     1981 gaaggcgatc gagtcggtgc agacgacctg ggcgccgtcg ggcgccgtct cgaccaccgt
     2041 ccggtgcgag atctcatccg ccggccgggc gacgggatcg cgctcgagca gcagctcgca
     2101 ggcctcggcg accgccatcc ccgccgtcac gccgacactc tcggcgggcg cgttgacgcg
     2161 gctgagccgg cccgaccgcc agacgtcgac gccgtcgccg agatggaccg acatcaccgc
     2221 cgcggcggca gcggggatcc cgagcgcctc gaggtaccag aggccggcga tgccggcgcc
     2281 gtccttgccg atcgcgcagt cgacgccgat cgccgcgcgc ggggcgcgct cggcgacgaa
     2341 gcgggccggc agcacgccgc agtacgacgc ggcgacgatg acgtcggcgg cgcggttggc
     2401 ctcggcgacg tggtaggccg agtcgaccgc gacgacccgg ccccgcgccg atgacgcgac
     2461 caccgtctgg cccggcgggc tgggcgcgcc gccggccgcg ctcacgacca gacgttctcc
     2521 cagcgcagct cgcggtcgag cagctgcttg tagtggcgct gcaggtagcc gacgtaggcg
     2581 tcgatgtgct cgcgcatccg gctctcggcg agcgcggcgt cgcgctgctc gatcgcctcg
     2641 aggatcgatc cgtggacctc cagcacgtgc tggcgcgagc gcttcgggta gtcgacgccg
     2701 agcgcggtcc cgtcggtgat ccagcccagc gaggcgagca ggtgctcgaa gagctggttg
     2761 cccgagacgt gcgcgatcag gtcgtggaag gcgcggttct cctcgaggaa gtccggcagg
     2821 ctgtcgagca cctcgcccat ccgctcgacc gacgcgcgca gctggtcgag ctgctcctcg
     2881 gagaccaaca cggcggcgcg cgcggccatc gccggctcga cgatctggcg cgcctcgacg
     2941 accgtgcggt agggcgcgcc ggtgacctgc agcagcagcg cgagcgtgct cgcgaggtag
     3001 cgcgggtcgg gcgaggtgac gaccggcccg ccgcgcgggc ccggcttgat cgtcagcacg
     3061 ccctggctct cgaggtagcg cagcgcctcg cgcagcgtcc cgcgcgcgac gccgtactcg
     3121 ttgagcatct cctgctcggg gggcagcatc acgcccggct cgaggccgcg ctcggtgatc
     3181 tccgagacga tccgctgggc gagcagcatc gcggtcttgc gcggcttgta gttgcgtcgc
     3241 gcgcgcgccg cgacgctgcc gctgatcagg acgctcacgc gcggcccccg gccggcaccg
     3301 gcagctcgtc gccggccagg ccgcgctcca gcgcgagccg gcgcagctcg gccttacgga
     3361 ccttccccga gggcgtcttg ggcaggtcgt cgaccagccg gacgtagcgc ggcagcgcga
     3421 acgccggcag tcgcctgacg caccagtcgt ggacgtcctc ccgtgacgcc ccatccgtgc
     3481 acaccgtaaa cagcatgacc tcgtcctctc ccgcgtcgac gtcggccgca accgcgatcg
     3541 ccgcacattc gattacccct gggtgatcca agaccgcctg ctcgatctcg tacgagctta
     3601 tgttctcgcc acgacggcgg atcgcgtcct tgagccgatc gacgaagtag aaccagccgt
     3661 cggcgtcgcg tcgcagcccg tcgccggtgt ggaaccagag gttgcgaaac gcctccgcgg
     3721 tcttgtcggc catcccgtag tagccgatgc aggtagtcca cggctgccgc ggacggacga
     3781 ccagctcgcc ggtctgcccg accgccacct cctcgtccgt ctcgggatcg acgagccgga
     3841 cgtcgaacca gtcggcgttg accttcccgc aggcgcccgc cgggcgctcg actccgtacg
     3901 gggtcatgat cggcatcgag gtctcggtca gcccgaagac ctcgacgaac gcgtcgatcc
     3961 cgaagcgctc gcggaatccg tcgaggatcg acgaggccgt cggcgccgcg aacacgcagc
     4021 ggagctcgtt ctcgccgtcg tcggggcgcg ccggctgctt ccagacgaag tccatcatca
     4081 cgccgacgaa gttcgtcacg gtcgcgccag agtcgcgcac ccagtcgatc cagcggctgg
     4141 cgctgaagcg ctcgcgcagc acgaagcgcg cgccggcgat cagcgccggc agcgccgcca
     4201 ggaactgcgc gttgccgtgg aagagcgggc cgaccgagag gtaggcgtcg gcgtcggtca
     4261 gccgcgtcag cgagacgcac tcgtcggcga agaagaccat ctgggcgtgc ggcatcatca
     4321 cgcccttcga gaggccggtc gtgccggagg tgaagaacac cgacgcgagg tcgtgcggcc
     4381 gcacctcggg cagcggcgcc ggcgtggtgg actccagcgc ggcgaacggc tcgaccgccc
     4441 agcccttggc ggccagggcc tgcgtcgcgg tcgcctggcg ctcggcgtcg ccgacgaggt
     4501 agaagcgctc gatcgtcgcg caggcctcgg cgctctcgac gaaccgctcg gcgagctcgg
     4561 cgtcgatcac cgcgaagcgc ggcgcggtcg tgcgcacctg gtgctcgagg aaggagccgc
     4621 ggtaggcggt gttgatcggg acctcggcca tgcccgcgca ggcggagccg aaccaggcga
     4681 agaccacctg cggcgagttc ggcgccatca ccagcagccg gtcgcccggc tcgccgccgg
     4741 cggcgatcag cccggaggcg atccgctcgg cgcgcgcgag gacctcggcg tagctgtgac
     4801 gctcgccggc gcccggcgcg tcgaggtaga tgctgtcgcc atgcgtccgc gcccgctcgc
     4861 gcagcaccca gggcaccgtc cagcaggcgc ggtccgcgaa ggtcggcgcg tactcctcgt
     4921 agacgttcac gcgtcctccc gcggcgcgcg cccggggaag cgcggcttgc gcttctcgag
     4981 gaacgcctgc gcgccctcgc gcatgtcctc ggagccggcg gcctgggcga gcatcccgag
     5041 cccggacagg aacgacccgc ggcaggcgtc ccaccagacg ttcgagctga tcttcgcgat
     5101 ctcgaggtag cgcgggctga gcgccagcag ctcgtccgcc cagcgcccga cctcggcctc
     5161 gagctcggca tcggcgacga ccgcgttgac catccccagc tcgtgcgcct ggcgcgcgtc
     5221 gtaggggcgg cagagcagcg agatctcctt ggcgcgcttc tcgccggcga tcagcgcgag
     5281 catgttcgtc ccgccgagca cgggcgcact gccaactcgc ggcccggtct ggccgaagcg
     5341 cgcgctctcg gcggcgatcg cgaggtcgca cgcgacgacg agctcgttgc cgccgccgaa
     5401 ggccgcgccg ttgaccgcgg cgatcacggg tcgcgggtgg cggcggatcg tgtcgtagag
     5461 gtcaagcgac gcctggaaca ggctgcgcag ctcggtctcg ctcgcctcgg cgaggttggc
     5521 caggtagccg ccggcgcaga acgccctgcc ggtcccggtg atcgcgacca cgcccgcatc
     5581 ggcggtcttg tcgagcgccg cgatcagctc ccgcgccgtc gcgaggtcgt aggcgttcat
     5641 ccgctcgggc acgtcgagcg tgacccaggc gacctcggcg cgccgttcga cgcgaacgac
     5701 gggccctccc tcggcgttga cgccgcgctc cgctgcagcc atgataatca ttatgcctca
     5761 aatgcccatc gctcgtctag aacccgcgta cctcgagctc cagcgggagg cgcgtgccct
     5821 ggccgagagc gtggagccct tcgctgccga ggccgacgag tcgagcgcgg tggacgcgcg
     5881 cgtgctcgac cggctgcgcg cgagcggcct ctgcggcgta gcggtgcccg ccggctgggg
     5941 cggccgctcg gagtcggtcg acccgctcgc ggtggcggtc gtccgcgagg cgctgatggg
     6001 cagctcctcg cagctcgact cgctgttcgc gctgcagggc atcggcagct acgcgatcgc
     6061 gcgcggcggc agcgacgccc agcgcgaccg ctggctgccc gacgtcgccg gtggtgcggt
     6121 gctcgcgggc ctggcgctca ccgagcgcga tgccggctcg gacctcaagg cggtcgccac
     6181 gacgctgcag ccgaccgccg acggcctgat gctgcgcggc gagaagtcgt tcatctcgaa
     6241 cgccggcgcc gcgggcttct acaccgtcct cgcgcgtgag gacgcgggcc tctcgctggt
     6301 gctggtcgaa gccgacgcgc ccggcgtctc ggtcacccgg acccaggaga tcatcgcccc
     6361 gcacgtgctc ggggacctcc acttcgacgg cgtgatgctg ccgccgtcgg cgcggatcgg
     6421 cgcgccgggg ggcggtctcg agctggtcct cgcgacgctc ggcgtcttcc gcgcgtcggt
     6481 cgccgcggcc gcggtcgggc tcgctcaggc ggcgctcgag gaggcgaccc gccacgcggc
     6541 gtcgcgcagc cagtttggcc gtccgctcgc ccgccacggc ccggtcgccg cgctgctcgc
     6601 cgactcgtgg acggagatcg agatggcgcg cctgctcacc taccgcgccg ccgagctcgc
     6661 gcgcgacgac ccgcagggcg cgctgaactg gtcgtcaatg gcaaagctgg cggcgaccga
     6721 gatggcctcg cgcgtcgtcg accgctcggt ccaggcgatg ggccgctggg gactctcgcg
     6781 cgaccgcaag atcgagcggc tctaccgcca ggcacgcccg atgcgtatct acgagggcgc
     6841 ctccgaggtg ctgcggctcg ggatcgcgcg aacgctggtg gacacgctcg aatgagcggc
     6901 tacggactcg acgggcgggt cgcgatcgtc accggcgcga gccgtgggct cggtctcgcc
     6961 gccgcccgtg ccctgctcgc ggagggcgtc cgcgtcgtcg ccgccgcgcg ctcggccgat
     7021 gcgctcgaca cgctcgcggc cgagcacccc ggccaggtcg aggcggtcgc gtgcgacatg
     7081 cgcgacgtcg acgccgtcgc ggcgctgccg gggatcgcgc tcgaccgctg cggcggcctc
     7141 gacgtgctcg tcaacaacgc cggcatcgcg ccggcggggg ccttcgacga gcagcgcctg
     7201 gagacctggc gcgaggtgct cgacgtcaac gtgctcgcgc cggtcgccct gacccgcgcc
     7261 gccgcccccc acctactcga ccagggcgcc ggcaaggtga tcaacgtcgc ctcgacctcg
     7321 ggcctgctcg gcaagccgac cctcgccgcc tactcggcct cgaagggcgc gctgatccag
     7381 ttcaccaagg cgctggcagc cgagtgggcc gcgcgcggga tccaggtcaa cgcgatcgcc
     7441 ccgggcgcct tcgagaccga cgcccagcag gcggtgctcg actccccgga ggtgctcgct
     7501 cgccggctcc gcaagatccc ggccgggcgg atgggccagc ccggcgagat cggcccgctg
     7561 gtctgctggc tcgcgtcgaa ccagtcggat ttcgtcagcg gcgcgacgct cgtgatcgac
     7621 ggcggtgaga gctcgaagct ctgacggatg gttatcgacg cccacaccca cgtctggccc
     7681 gacgcgatcg ccgggcgcgc gctcgcgggc tcggttcgcg acctcgagcg cttcggcgac
     7741 ggacgcgtcg acagcctgat cgccacgatg gacgcggccg gcatcgaccg ctcggtctgc
     7801 ctcggcgtgg cgaacagcgg cgaccgcgtc gaggccgcca accgcttcgc aggctcgctc
     7861 gaccgcgacc ggctgatcgg cttcgggtcg atccaccccg acctcagcgc ggccgagaac
     7921 gtcgcgagcc tgcgtcgcca cggcctcgtc ggcgccaagt cgcacccgct cttccagagc
     7981 tactcgctcg acgacccgcg gctgctcgag atcctcgacg ccctgcgcgg cgagttcgcc
     8041 gtgatcgtcc acgtcgggcc ggcgggcgcc gactccggcg cccgctgtac gccggcgatg
     8101 atccgcgaca tcgcccgcca gctgcccggg ctggcgctga tcgcctgtca cctcggcggc
     8161 tatcgcgtgc tcgacgaggc cgaggacagc gtcgtcggat tgcccggcgt ctacctcgac
     8221 acctcatggc cgccgagcat cgcgacgctc gaccgcaacc gcgtccgcgc ggcgatcgag
     8281 cgccatgggc cggagcgggt gctgttcgcc tccgactggc cgatggccga tccaggggcc
     8341 gagcttgcgg cggtcgaggc gctcgggctg tccgacgcgg acacgcgggc gatcctcggc
     8401 ggcaacctcg tccgtctgct cagtgagttg ccggcgtcgt cagcgggcgg gcccgccacc
     8461 gactgacgct cgcgccgtaa gcgagcgggc gagctcggcc agctcacgga ccgccggcgt
     8521 ctcatcggcg ggccgccacg ccagcacgag cggcgacggc tcgatgtcct cgaccgtcgt
     8581 gaagcgcacc cccgcggtcg tcggaccgag cgccgcggcg atcgacgccg ggacgatcgc
     8641 gatcgcctgg cccgcggcca cgaggtggag caggtcgtcc cagccgcgcg cccgtccgcc
     8701 gatccgcg
//
LOCUS       DATABV010000227         8390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_880123, whole genome shotgun sequence.
ACCESSION   DATABV010000227 DATABV010000000
VERSION     DATABV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880123"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VHF88_10470"
     CDS             <1..246
                     /locus_tag="VHF88_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162229.1"
                     /translation="LPNVLRYPFDETNVLVVAGEGPVSVGRLRAAEGRLPPALRSVAR
                     DAAARSGPPLAGGEVYTDDHAPVEWLIDKSIVDYAAD"
     gene            complement(248..1159)
                     /locus_tag="VHF88_10475"
     CDS             complement(248..1159)
                     /locus_tag="VHF88_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2162230.1"
                     /translation="MRRVVLAVLVLIAIVLAINTVTTDRQTKAAAADVGQIVDTRFGD
                     VQVRERGPARAPAIVLIHCYTCSIHWWLKLEPLLTGDRRVVSVDLLGHGGSEKPLEGY
                     SAENQADAVAAALRRRGVRDALLVGQSLGGPIATAVAERNPGLARGVVIMDSSPREGY
                     GDLPFTQKIGTAPVVGQAAWHTTPDFMIRDGLADAFAEGFEVPDQFVEDVRRMTFTSF
                     KEADKLPGYQDTPLPERLTATGLPVLVIFGEEDTVIVPPDEAADEYRAIPGARVALLP
                     GVGHTPQVEAPRRTAALLRSFDRGTMR"
     gene            complement(1217..1888)
                     /gene="bioD"
                     /locus_tag="VHF88_10480"
     CDS             complement(1217..1888)
                     /gene="bioD"
                     /locus_tag="VHF88_10480"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX2162231.1"
                     /translation="MRGVFVTGTDTGVGKTVVAGAICAALAARGERVAAFKPVVTGTA
                     EESGDWPPDHELLASAASAGQSTIEVAPVTFGPPVSPHYAAELAGSEIDAAQLRDTAC
                     SAAAGVDALVCEGVGGLLVPLTLDYSVRDLAADLGVPLVIVARTGLGTINHTLLTVAA
                     ARSAGLHVAGVVMTPWAADPEPIERSNRATVERLSGVAVSALPHATPATLAAAGAELP
                     LDDWL"
     gene            complement(1897..3147)
                     /gene="bioF"
                     /locus_tag="VHF88_10485"
     CDS             complement(1897..3147)
                     /gene="bioF"
                     /locus_tag="VHF88_10485"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX2162232.1"
                     /translation="MTDIESALEDIRESGLYRRMRMISGPQGPRVLLDGRPVLLLCSN
                     NYLGLADHPRVREAAAEAAMRYGAGAGASRLVSGNMRIHRRLEERLSEFKGYRSALLF
                     GSGFLANTGVVQALAKPGEVVFSDALNHASIVDGCRLAGAETFVYDHADIDHLAWGLR
                     EAEGRGSLIVTDGVFSMDGDVAPLAEIVELARRFDARVMVDDAHGTGTIGPDGRGSVA
                     AAGLEDEVDVLVGTLGKSLGSYGAYVCCDKAMAKYLVNTARTLIFSTALAPPQVAAAM
                     AALELLRAEPRRVERLQRNARILREALAESGLPAGAGDTPIVPVVVGDADSAVSASER
                     ALERGVFAQAIRPPTVPDGSSRLRLTVMASHTKSELRDAAKVVAKAVPETVRKAAERR
                     ALEADVAAAQAAGGRVFDGLADAA"
     gene            complement(3144..4307)
                     /gene="bioB"
                     /locus_tag="VHF88_10490"
     CDS             complement(3144..4307)
                     /gene="bioB"
                     /locus_tag="VHF88_10490"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX2162233.1"
                     /translation="MPPLISKDEALRLGEIEDRAEIEALIERAWEARVEHFGDSTDMC
                     SLVNAKSGGCAEDCGFCAQSRFAEAETPMHAMMSPEQILEHARAAEAAGAHRFCMVTQ
                     GQGLSKRDFENILEGARLVAEQTNLKRCASIGHMSVKRAKQLKEAGIQRVHHNVETAE
                     SYYPEVSTTVRYEGRLRTIEAVEQAGLETCVGGILNLGESREQRVEMAFELAKIDPTS
                     VPINMLNPRPGTKFGDRDYMDPWEAVKWIAIFRLILPNALFRLCGGRVENLGELHALA
                     VKAGINGVMMGNFLTTLGSEPADDRAMFEELGLNVARQHDNGANPRPDNRSGWLDGET
                     PRTPIDELVDSQQQAPFWNPETQLRRVKKDKVPPRPDGAPNRGLKVVQVEGAR"
     gene            4478..5560
                     /locus_tag="VHF88_10495"
     CDS             4478..5560
                     /locus_tag="VHF88_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX2162234.1"
                     /translation="MTPKVAPLRRRALKAVEALFTPLLPDDYLELINPLWSTRELRGR
                     IERIEPETANAVTVTIRPGHEWQGHLPGQYLRVGIVVDGRHHWRAYSLTSDPERSDGW
                     ITITPKRYEPGVVSPYLTRQARPGEIVRLGGVEGTFTLPDPLPRSMLFVSAGSGITPV
                     MSMLRNIERRGALHDVLLVHSARTRDDFIFGDRLRSMAERNAGFRLVERHTAEQGRMS
                     VDDIDALCPDWRERETFVCGPREMIDALCERWQAEGLADRLRMERFQPIIGGEVEPGL
                     GGTIRFLRSGKETACDGSTPILVAGEAAGIEMPFGCRMGICHTCTGRLCSGELRDLRT
                     GEIHGVDGEMVRTCVNAPEGPVEIAL"
     gene            5581..6717
                     /locus_tag="VHF88_10500"
     CDS             5581..6717
                     /locus_tag="VHF88_10500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX2162235.1"
                     /translation="MATIEAERPVQTESPLARLSDEQLEQLGREFDAIHDEVYAELGD
                     RDRRYIQSMIEMHRRLAVLGRLLLWGSKYRPAQVAGTACLSLAKILENMEIGHNVMHG
                     QWDWMNDPQIHSTTWDWDTASTPEAWKHSHNYVHHTYTNILGKDRDLGYEIMRIDPDQ
                     KWHPVYLAQPLYNLLLAAFFEWGVALHDLDFEAIRKGEKSKKQLKRELKGMWGKARSQ
                     IVKDYIAYPALSGRHWKRTLAGNFTSNIVRNVWSYAIIFCGHFPDQAYTFKPEEVEGE
                     TRGGWYVRQLLGAANIEGSPLFHVISGNLGYQVEHHLFPDMPSSRYAEIAPKVKDICE
                     RYGLPYNSGPFSQQLGMVQRTILRLALPGGKRRPKPGPYRAEAA"
     gene            complement(6719..7750)
                     /locus_tag="VHF88_10505"
     CDS             complement(6719..7750)
                     /locus_tag="VHF88_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX2162236.1"
                     /translation="MSYGDHITRNRGAIRFLLFALGVPQALIGLWALLAPGSFHDDFP
                     VGTAGWVDVLGPFDEHLVTDVGALFVGLGFLLCFAAVSLRRGTVIAAAISWLIFAIPH
                     LVWHVFNLEPYSTGDAIGNVVTLVWTVVGGLLVLVLLRTRPAQSTATAGVDGARVAGV
                     PDSRAGLMARSAYSYSRRAVGQVMEPTRVYAHHPTLLAGYGALEYATEKADRVPRDLK
                     ALASTKAAALAGCEFCIDIASSLATKEGVAEAKLRALPDHRTSPAFSEVERLVLDLAV
                     GMTRTPVDVSDELFARLREHFDEAQLVELANEIAIENYRARFNWAFGIGSQGFAEGAV
                     CARAEAVPA"
     gene            complement(7841..>8390)
                     /locus_tag="VHF88_10510"
     CDS             complement(7841..>8390)
                     /locus_tag="VHF88_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2162237.1"
                     /translation="VAHPFHDAIEALEAYPYPVIAQINGHAIGGGLELALTCDIRIAK
                     RGIRVGMPPAKLGLIYSHTGLRKFVDVCGPANTAELFYVGRNLEAERGLEIGLLNHVV
                     EPDELEATAVSMAGEIAANAPLSLAGNKRILHELRAHAAALPADVERELVELRESCFR
                     SEDFREGVRAFGEKRAPSWQGR"
BASE COUNT         1216 a         2945 c         3031 g         1198 t
ORIGIN      
        1 ctgccgaacg tcctgcgcta cccgttcgac gagaccaacg tgctggtggt ggccggggag
       61 gggccggtct cggtcggtcg gctgcgcgca gcggaggggc ggctgccccc ggcgctgcga
      121 tcggtcgcgc gcgacgcggc ggcacggtcg ggcccgccgc tggcgggcgg cgaggtctac
      181 accgacgacc acgccccggt cgagtggctg atcgacaagt cgatcgtcga ctacgccgcc
      241 gactgagtca gcgcatggtg ccgcggtcga acgatcgcag cagggcggcg gtcctgcgcg
      301 gcgcctcgac ctggggcgtg tgcccgacgc ccggcagcag cgccacgcgt gcgccaggga
      361 tagcgcgata ctcgtcggcc gcctcgtccg gcggcacgat gacggtgtcc tcctcgccga
      421 agatcacgag gaccggcagt cctgtcgcgg tcagccgctc gggcagcggc gtgtcctggt
      481 agccgggcag tttgtcggcc tccttgaagg acgtgaacgt catccggcgg acgtcctcga
      541 cgaactggtc cggcacctcg aagccctcgg cgaaggcgtc ggcgaggccg tcacggatca
      601 tgaagtcggg cgtcgtatgc catgcggcct ggccgacgac cggcgccgtt ccgatcttct
      661 gcgtgaaggg caggtcgccg tagccctcgc gcggcgacga gtccatgatc accacgccac
      721 gggcgagccc gggattgcgc tcggcgacgg ccgttgcgat cggccctccg agcgactggc
      781 cgaccagcag agcgtcgcgt accccgcggc ggcgcagagc ggcggcgacc gcgtcggcct
      841 ggttctccgc cgagtacccc tcgagcggct tctccgagcc gccgtggccg agcaggtcga
      901 ctgagacgac gcggcggtcg ccggtcagca gcggctccag cttcagccac cagtggatcg
      961 agcaggtgta gcagtggatc agcacgatcg ccggggcgcg cgccggcccg cgctcgcgga
     1021 cctggacgtc gccgaagcgc gtgtcgacga tctgcccgac gtccgccgcg gccgccttgg
     1081 tctggcggtc ggtggtcacc gtgttgatcg caagcacgat cgcgatcagc acgagcacgg
     1141 ctaggacgac tcggcgcatc tgcttctccc gacctctcgc gcgcggctcc cgtacgcggc
     1201 gctggaggcc gcgagcctac agccagtcgt cgagcggcag ctcggctccg gcggccgcga
     1261 gcgtcgccgg cgtcgcgtgg ggcagcgcgc tgaccgcaac cccgctcagg cgctcgacgg
     1321 tcgcgcggtt ggaccgctcg atcggctcgg ggtccgcggc ccagggcgtc atcacgacgc
     1381 cggcgacatg caggccggcc gagcgggcgg ccgcgacggt cagcagcgtg tggttgatcg
     1441 tgccgaggcc ggtgcgggcg acgatcacca gcggcacgcc gaggtcggcc gcgagatcgc
     1501 gtaccgagta gtcgagggtg agggggacga gcaggccgcc gacgccctcg cagacgagcg
     1561 cgtcgacgcc ggctgcggcc gagcaggcgg tgtcgcggag ctgcgccgcg tcgatctcgc
     1621 tgcccgccag ctcggcggcg tagtgcggcg acaccggcgg gccgaaggtc accggcgcga
     1681 cctcaatcgt gctctggccg gcgctcgccg ccgaggccag cagctcgtga tccggcggcc
     1741 agtcgccgct ctcctccgcg gtgccggtca cgaccggctt gaaggcggcc acccgctcgc
     1801 cgcgagccgc gagcgcggcg cagatcgctc cggcgacgac cgtcttgccg acgcccgtgt
     1861 cggtgccggt aacgaagacg ccgcgcatcg gcggcgctag gccgcgtctg cgaggccgtc
     1921 gaagacgcgc ccgccggcgg cctgcgccgc ggcgacgtcg gcctcgaggg cacggcgctc
     1981 tgccgccttg cgcaccgtct cgggaaccgc cttggcgacg accttggcgg cgtcgcgcag
     2041 ctccgacttc gtgtgcgagg ccatcaccgt caggcgcagt cgcgacgagc cgtcgggaac
     2101 cgtcggcggg cggatcgcct gcgcgaagac cccccgctcg agcgcacgct cgctggcgga
     2161 gaccgcagag tcggcgtcgc cgacgacgac cggcacgatc ggcgtgtcgc cggcgccggc
     2221 cggcaggccg ctctcggcga gcgcctcgcg caggatgcgc gcattgcgct gcaggcgctc
     2281 gacccgtcgc ggctcggccc gcagcagctc cagggctgcc atcgctgcag cgacctgcgg
     2341 cggggccagc gcggtcgaga agatcagcgt gcgggcggtg ttgacgagat acttcgccat
     2401 cgccttgtcg cagcagacgt acgcgccgta ggagccgagc gacttgccga gcgtgccgac
     2461 cagcacgtcg acttcgtcct cgagcccggc ggcggcgacc gagccgcgcc cgtccggtcc
     2521 gatcgtgccg gtcccgtgag cgtcgtcgac catcacgcgc gcgtcgaagc gccgcgccag
     2581 ctcgacgatc tcggccagcg gtgcgacgtc gccgtccatc gagaagacgc cgtcggtgac
     2641 gattaggctg ccgcggccct ccgcctcgcg caggccccac gccaggtggt cgatgtcggc
     2701 gtggtcgtag acgaacgtct ccgcgcccgc gagccggcag ccgtcgacga tgctcgcgtg
     2761 gttcagcgcg tcggagaaga cgacctcgcc gggcttggcg agcgcctgca cgacgccggt
     2821 gttggcgagg aagcccgagc cgaacagcag cgcgctgcgg tagcccttga actcggagag
     2881 gcgctcctcg agccggcggt ggatgcgcat gttgcccgac accagtcgcg aggcgccggc
     2941 gcccgcgccg tagcgcatcg ccgcctcggc ggcggcctcg cgcacgcgcg gatggtcggc
     3001 caggccgagg tagttgttcg agcacagaag caggacgggc cgcccgtcca acaggacgcg
     3061 cggcccctga ggccccgaga tcatccgcat ccggcggtag agcccgctct cgcggatgtc
     3121 ctcgagcgcg ctctcaatgt cggtcatcgc gccccctcca cctggacgac cttgagcccg
     3181 cggttcggcg cgccgtcggg ccgtggcggc accttgtcct ttttgacgcg gcgcagctga
     3241 gtctcggggt tccagaacgg cgcctgctgc tggctgtcga cgagctcgtc gatcggggtg
     3301 cgcggcgtct cgccgtcgag ccagccgctg cggttgtccg gccgcgggtt ggcgccgttg
     3361 tcgtgctggc gcgcgacgtt cagcccgagc tcctcgaaca tcgcccggtc gtcggccggc
     3421 tcggagccga gcgtcgtcag gaagttgccc atcatcacgc cgttgatccc ggccttgacc
     3481 gccagcgcat gcagctcgcc gaggttctcg acccgcccgc cgcagagccg gaagagcgcg
     3541 ttgggcagga tcagccggaa gatcgcgatc cacttgacgg cctcccacgg gtccatgtag
     3601 tcgcggtcgc cgaacttcgt ccccgggcgt gggttgagca tgttgatcgg cacgctggtc
     3661 gggtcgatct tcgcgagctc gaaggccatc tcgacacgct gctcgcgcga ctcgccgagg
     3721 ttcaggatgc cgccgacgca cgtctcgagt cccgcctgct cgaccgcctc gatcgtccgc
     3781 agccgcccct cgtagcggac cgtcgtcgag acctccgggt agtagctctc cgccgtctcg
     3841 acgttgtggt ggacccgctg gatgccggct tccttgagct gcttggcgcg cttgaccgac
     3901 atgtggccga tcgaggcgca gcgcttgagg ttggtctgct cggcgaccag ccgcgcgccc
     3961 tcgaggatgt tctcgaagtc gcgcttggac agcccctggc cctgggtgac catgcagaag
     4021 cggtgggcgc ccgcggcctc cgcggcgcgt gcgtgctcga ggatctgctc cgggctcatc
     4081 atcgcgtgca tcggcgtctc ggcctcggcg aagcgcgact gggcgcagaa gccgcagtcc
     4141 tcggcgcagc cgcccgactt cgcgttgacc agcgagcaca tgtcggtcga gtcgccgaag
     4201 tgctcgacgc gcgcctccca cgctcgctcg atcagggcct cgatctcggc ccgatcctcg
     4261 atctcgccga ggcggagcgc ttcgtctttg ctgatcagcg ggggcatggc atctccctca
     4321 gggtccggat tggcctggaa actagggccg cgcccggacg gagcggccga ggcccgtagc
     4381 cggcgatgtc cctgatttgc gaccggaagg ttgccttgtc gatccatcgg gcattctccg
     4441 aggatggtgc gggagcccga ccttggctga gcgcggcgtg acgccgaagg tcgcgccgct
     4501 gcgacggcgg gcgctcaagg cggtggaggc gctcttcacg ccgctgctgc ccgacgacta
     4561 cctcgagctg atcaacccgc tctggtccac gcgcgagctg cgtggtcgga ttgagcggat
     4621 cgagcccgag accgcgaacg cggtgacggt cacgatccgt ccgggacacg agtggcaggg
     4681 ccatctgccc ggccaatacc tgcgcgtcgg gatcgtcgtc gacggtcgcc accactggcg
     4741 ggcgtattcg ctgacctccg accccgagcg ctcggacggc tggatcacga tcacgccgaa
     4801 gcgctacgag ccgggcgtgg tgtcgcccta cctgacgcgt caggcgcggc ccggcgagat
     4861 cgtccggctc ggcggggtcg aggggacgtt cacgctgccc gatccgctgc cgcgctcgat
     4921 gctgttcgtc agcgcgggca gcggcatcac accggtgatg agcatgctgc gcaacatcga
     4981 gcggcgcggc gcgctgcacg acgtgctgct cgtccactcc gcgcgcaccc gcgacgactt
     5041 catcttcggc gaccggctgc gctcgatggc ggagcgcaac gccggcttcc ggctcgtcga
     5101 gcggcacacc gctgagcagg gccgcatgtc ggtcgacgac atcgacgcgc tgtgcccgga
     5161 ctggcgcgag cgcgagacgt tcgtctgcgg cccgcgcgaa atgatcgacg cgctctgcga
     5221 gcgctggcag gccgaggggc tcgccgaccg tctccgcatg gagcgcttcc agccgatcat
     5281 cggcggtgag gtcgagcccg gcctgggcgg gacgatccgc ttcctgcgca gtggcaagga
     5341 gaccgcgtgc gacggctcga cgccgatcct cgtcgccggc gaggcggcgg ggatcgagat
     5401 gccgttcggc tgccggatgg gcatctgcca cacctgcacc gggcggctgt gctccggcga
     5461 gctgcgcgac ctgcgcaccg gtgagatcca cggcgtcgac ggcgagatgg tccggacctg
     5521 tgtcaacgcc cccgagggcc cggtcgagat cgcgctctga gcaactagag aggaatcgag
     5581 atggcaacga tcgaggccga gaggccagtt cagacggaga gcccgctggc gcggctcagc
     5641 gacgagcagc tcgagcagct cgggcgcgag ttcgacgcga tccacgacga ggtctacgcc
     5701 gagctcggcg atcgcgaccg ccgctacatc cagtcgatga tcgagatgca ccgccgcctg
     5761 gcggtgctcg gccggctgct gctctggggc agcaagtacc gcccggcgca ggtcgccggg
     5821 accgcgtgcc tgtcgctggc caagatcctc gagaacatgg agatcggcca caacgtgatg
     5881 cacggccagt gggactggat gaacgacccg cagatccact cgacgacgtg ggactgggac
     5941 accgcgtcga cacccgaggc ctggaagcac tcgcacaact acgtccacca cacctacacc
     6001 aacatcctcg gcaaggaccg cgatctcggc tacgagatca tgcgcatcga cccggaccag
     6061 aagtggcacc cggtctacct cgcccagccg ctctacaacc tcctcctggc ggccttcttc
     6121 gagtggggcg tggcgctcca cgatctcgac ttcgaggcga tccgcaaggg ggagaagtcg
     6181 aagaagcagc tcaagcgcga gctgaaaggg atgtggggca aggcgaggag ccagatcgtc
     6241 aaggactaca tcgcctaccc ggcgctcagc ggccgccact ggaagcgcac gctcgcgggc
     6301 aacttcacct cgaacatcgt gcgcaacgtg tggtcctacg cgatcatctt ctgcggccac
     6361 ttccccgacc aggcctacac gttcaagccg gaggaggtcg agggcgagac gcgcggcggc
     6421 tggtacgtgc gccagctgct cggggcggcg aacatcgagg gcagcccgct gttccacgtg
     6481 atcagcggca acctcggcta ccaggtcgag caccacctct tcccggacat gccgagctcg
     6541 cgctacgccg agatcgcccc caaggtcaag gacatctgcg agcgctacgg gctgccgtac
     6601 aacagcggcc cgttcagcca gcagctgggc atggtccagc ggacgatcct gcggctggcg
     6661 ctccccggcg gcaagcggcg gccgaagccg ggcccgtatc gcgcggaggc ggcttgattc
     6721 atgccggcac cgcctccgcc cgggcgcaga cggcgccctc ggcgaagccc tgggagccga
     6781 tcccaaaggc ccagttgaag cgggcgcggt agttctcgat cgcgatctcg ttggcgagct
     6841 cgacgagctg ggcctcgtcg aagtgctcgc gcaggcgcgc gaagagctcg tccgagacgt
     6901 cgaccggcgt gcgcgtcatc ccgaccgcga ggtcgagcac cagccgctcg acctcgctga
     6961 acgccgggct ggtgcggtga tccggcagcg cgcgcagctt cgcctccgcg acgccctcct
     7021 tggtggcgag gctcgaggcg atgtcgatgc agaactcaca gccggccagc gccgccgcct
     7081 tcgtcgatgc gagcgccttc agatcgcgcg gcacgcggtc ggccttctcg gtcgcgtact
     7141 cgagtgcgcc gtaaccggcg agcagcgtcg ggtggtgggc gtagacgcgc gtcggctcca
     7201 tcacctggcc gaccgcgcgg cggctgtagg agtaggcgga gcgagccatc aggcccgcgc
     7261 ggctgtctgg gacgccggcg acgcgagcgc cgtcgacgcc ggccgtggcc gtgctctgcg
     7321 ccggccgggt gcggaggagg actagcacga gcaggccgcc gacgacggtc cagacgagcg
     7381 tgacgacgtt gccgatcgcg tcgccggtcg agtacggctc caagttgaag acgtgccaga
     7441 cgaggtgggg gatcgcgaag atcagccatg agatcgctgc ggcgatcacc gtgccgcggc
     7501 gcaggctgac cgccgcgaag cagagcagga agccgagccc gacgaacagt gcgccgacgt
     7561 cggtgacgag gtgctcgtcg aaaggcccga ggacgtcgac ccagcctgcc gtgccgaccg
     7621 ggaagtcgtc atgaaaggag cccggcgcga gcagggccca cagcccgatc agcgcctgcg
     7681 gcacgccgag cgcgaacagc aggaagcgga tcgctccgcg gttgcgggtg atgtgatcgc
     7741 cgtagctcat ctcggtgcct cctatttcgc cggtcggcca aaccgtacgc cccgcccggg
     7801 cccggcgtcg acgggatgcg gcgagagcgc gacagaggcg ctagcggccc tgccagctgg
     7861 gggcgcgctt ctcgccgaag gcgcggacgc cctcgcggaa gtcctcgctg cggaagcagg
     7921 actcgcgcag ctcgaccagt tcgcgctcga cgtcggcggg cagggcggcg gcgtgggcgc
     7981 gcagctcgtg caagatgcgc ttgttgccgg cgagcgacag cggcgcgttg gcggcgatct
     8041 cgcccgccat cgagacggcc gtcgcttcga gctcgtccgg ctcgacgacg tggttgagca
     8101 ggccgatctc gagtccgcgc tcggcctcga ggttgcggcc gacgtagaag agctcggccg
     8161 tgttcgcggg cccgcagacg tcgacgaact tgcgcaggcc ggtgtgcgag tagatcaggc
     8221 caagcttggc cggcggcata ccgacgcgga tgccgcgctt cgcgatccgg atgtcgcagg
     8281 tcagcgccag ctcgagtccg ccgccgatcg cgtggccgtt gatctgcgcg atcaccggat
     8341 agggataggc ctcgagcgcc tcgatcgcgt cgtgaaacgg gtgggcgacc
//
LOCUS       DATABV010000228         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_398636, whole genome shotgun sequence.
ACCESSION   DATABV010000228 DATABV010000000
VERSION     DATABV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398636"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /gene="ilvN"
                     /locus_tag="VHF88_10515"
     CDS             complement(<1..222)
                     /gene="ilvN"
                     /locus_tag="VHF88_10515"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX2162238.1"
                     /translation="MGEPATQDILNLDDLRASGSGHTGRKHILSLLVENKPGVLARIA
                     GLFARRGFNIDSLAVGPTDDATISRLTLTV"
     gene            complement(226..1902)
                     /gene="ilvB"
                     /locus_tag="VHF88_10520"
     CDS             complement(226..1902)
                     /gene="ilvB"
                     /locus_tag="VHF88_10520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX2162239.1"
                     /translation="MRAVDAIMECLKAEGVEHVFGLPGGANLPTYDALYDAGLRHIQC
                     RHEQGAGHAAEGYAKASGRVGVALATSGPGATNLVTAIADAVMDSVPTVFITGQVRTD
                     LLGTDGFQEADVTGITMPIVKHSLMIRDPQDIPVAIHEAFHIARTGRPGPVLVDIPQD
                     LSKADIKYEPVDTLDLPGYQPTAEGNVKQIKLAAKALANSRRPVIYAGGGVVNANASK
                     ELVELCLSDRFPVTCTLMGLGAFPAPHEQWLGMLGMHGTRAANYSMDEADLIVAIGAR
                     FDDRITGKLSEFAPRAKFVHIDVDPAEISKNVPAHIPIVGDAKRILPKLTTEYRALKA
                     DSGRLDGWWQRIEGWREKHPLAYEDSTDSEIKPQRMIEALYEVTGGDAIVTSDVGQHQ
                     MWTAQYFHFDGPRKWINSGGLGTMGFGLPAAMGAKVACPDENVVCVAGDGSVAMNIQE
                     LATCVTEKIPIKVFIMNNGYLGMVRQWQELFWDRRYSSVDMGSSPDWVKLAEAYGATG
                     LRVDDKGELTDVMKAAMEAEGPVVCDVRVTPEENCYPMIPAGQAARDMVG"
     gene            complement(2001..2252)
                     /locus_tag="VHF88_10525"
     CDS             complement(2001..2252)
                     /locus_tag="VHF88_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162240.1"
                     /translation="MEVLVILFFFGLSAGVVAKIKGSSFLIWFMVGAVLPGLGTIAAA
                     LYRSERGEPQRRCPECGNLVGLSTQVCTRCGRDLDFPQE"
     gene            complement(2274..2444)
                     /locus_tag="VHF88_10530"
     CDS             complement(2274..2444)
                     /locus_tag="VHF88_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162241.1"
                     /translation="MGEAVRTGTRKLLALAVLLVAAWILFKIVLGFVTAVAWIAVAVL
                     AVVAVVWAIRVL"
     gene            complement(2474..>3351)
                     /locus_tag="VHF88_10535"
     CDS             complement(2474..>3351)
                     /locus_tag="VHF88_10535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2162242.1"
                     /translation="AVHHTATRMGLIEGAPPTAAQIESEWAAQREVVEFVAAQPGGTY
                     WVCEDAGEPVGYARVCRFDEMEQLTELMVLPGHHGRGIGRGLLERCFPADPTPELARL
                     VVAAGATGDLTLYSDFGVMPVTGHWHLRASAADYAERRAREIDAADPAVHALEGDRAV
                     AEWKALEPPAVGHRRPALHEFFARTRSCLATMDPDSGSATGLCWVGPTGDVGPAVAAS
                     PAELVPVVLAALDRVAKTIEPPSFGVYCATDSWWLLRRLRKLGFKVHWPGWVMSSVPL
                     PGLDRYMPMRPPHLL"
BASE COUNT          497 a         1151 c         1201 g          502 t
ORIGIN      
        1 gaccgtcagc gtcaggcgcg agatcgtcgc gtcgtcggtc gggccgacgg cgagcgagtc
       61 gatgttgaag ccgcgccgtg cgaacagccc cgcgatcctg gcaaggacgc ccggcttgtt
      121 ctcgaccagc agcgagagga tgtgcttgcg gccggtgtgg ccgctccccg acgcgcgtag
      181 gtcgtcgaga ttgaggatgt cctgggtggc aggctcgccc atcgctcagc ccaccatgtc
      241 ccgcgcggcc tggccggcgg ggatcatcgg atagcagttc tcctcgggcg tcacgcggac
      301 gtcgcagacg accgggccct cggcctccat cgcagccttc atcacgtcgg tcagctcccc
      361 cttgtcgtcc acgcgcaggc cggtcgcgcc gtaggcctcc gcgagcttga cccagtcggg
      421 gctggagccc atgtcgacag agctgtagcg gcggtcccag aacagctcct gccactggcg
      481 gaccatgccc aggtagccgt tgttcatgat gaagaccttg atcgggatct tctcggtcac
      541 gcaggtcgcg agctcctgga tgttcatcgc gaccgagccg tcgccggcaa cgcagacgac
      601 gttctcgtcc gggcaggcca ccttggcccc catcgcggcc ggtaggccga agcccatcgt
      661 gccgagtccg ccggagttga tccacttgcg cgggccgtcg aagtggaagt actgcgcggt
      721 ccacatctgg tgctggccga cgtcggaggt gacgatcgcg tcgccgccgg tgacctcgta
      781 gagcgcctcg atcatccgct gcggcttgat ctcggagtcg gtggagtcct cgtaggcgag
      841 cggatgcttc tcgcgccagc cctcgatccg ctgccaccag ccgtcgagcc gaccgctgtc
      901 ggccttcagg gctcggtact cggtcgtcag cttcggcagg atccgcttcg catcaccgac
      961 gatcgggatg tgcgccggca cgttcttcga gatctcggcc ggatcgacgt cgatgtggac
     1021 gaacttcgcg cgtggcgcga actcggacag cttgccggtg atccggtcgt cgaagcgggc
     1081 gccgatcgcg acgatcaggt cggcctcgtc catcgagtag ttggcggcgc gcgtgccgtg
     1141 catgccgagc atcccgagcc actgctcgtg cggcgccggg aacgcgccga gccccatcag
     1201 cgtgcaggtg accgggaagc ggtcggacag gcagagctcg acgagctcct tcgacgcgtt
     1261 ggcgttgacg acgccgccgc cggcgtagat caccggccgg cgagagttgg cgagcgcctt
     1321 cgcggccagc ttgatctgct tgacgttgcc ctcggccgtc ggctggtagc cgggcaggtc
     1381 gagcgtgtcg accggctcgt acttgatgtc ggccttcgag aggtcctgcg ggatgtcgac
     1441 gagcaccggc ccggggcggc cggtgcgcgc gatgtggaag gcctcgtgaa tcgcgaccgg
     1501 gatgtcctgg gggtcgcgga tcatcagcga gtgcttgacg atcggcatcg tgatgccggt
     1561 cacgtcggcc tcctggaagc cgtcggtgcc gagcaggtcg gttcggacct gaccggtgat
     1621 gaagacggtc ggcaccgagt ccatcaccgc gtcggcgatc gcggtcacga ggttggtcgc
     1681 tccggggccg gaggtcgcca gcgcgacgcc gacgcggccc gaggccttcg cgtagccctc
     1741 cgccgcatgg cccgcgccct gctcgtggcg gcactggatg tggcgcaggc cggcgtcgta
     1801 gagcgcgtcg taggtcggca ggttggcgcc acccgggagg ccgaatacat gctcgacgcc
     1861 ctcggccttg aggcactcca tgatggcgtc gacagctcgc attgcggtgc tccttctccg
     1921 gccgggctcg aaagtgggcg ggcggcgccg ggggccgccg gacaagcgat ggtaccaggg
     1981 ctttttccgc tctccgacgg ttattcctgc gggaagtcca agtcgcggcc gcaccgtgtg
     2041 cagacctgcg tggagaggcc gacgaggttg ccgcactccg ggcagcggcg ctgcggctcg
     2101 ccgcgctcgc tgcggtagag cgccgcggcg atcgtcccaa gtccgggcag gaccgcgccg
     2161 accatgaacc agatcaggaa cgaggagccc ttgatcttgg cgacgacccc ggccgagagg
     2221 ccgaagaaga aaaggatgac caggacctcc accgcggctt taggggcgcc ggctcagagg
     2281 acgcggatcg cccacacgac ggcgacgacc gccagcaccg cgacggcgat ccacgcgacc
     2341 gcggtcacga agccgagcac gatcttgaag aggatccagg cggccaccaa cagcaccgcg
     2401 agcgccagca gtttcctcgt acctgtcctg acggcctcgc ccaccagccg tagcttagtg
     2461 accgcgcctg ccgtcagagc agatgaggag gcctcatggg catgtaacgg tcgaggcccg
     2521 gcagcgggac cgagctcatc acccagccgg gccagtggac cttgaagccg agcttgcgca
     2581 gccggcgcag cagccaccag ctatcggtcg cgcagtagac gccgaagctg gggggctcga
     2641 tcgtcttggc gacccgatcg agcgcggcga gcacgaccgg gaccagctcg gccggcgagg
     2701 ccgcgaccgc cgggccgacg tcgccggtcg ggccaaccca gcagagaccg gtcgcgctgc
     2761 cgctgtccgg atccatcgtc gcaaggcacg accgcgtgcg ggcgaagaac tcgtgcagcg
     2821 cgggccggcg atggccgacc gcgggcggct cgagcgcctt ccactcggcg acggcgcggt
     2881 cgccctcgag cgcgtggacc gccggatcgg cggcgtcgat ctcgcgcgca cggcgctcgg
     2941 cgtagtcggc cgcggacgcg cgcaggtgcc agtggccggt gaccggcatc acgccgaagt
     3001 cggaatagag ggtcaggtcg ccggtcgccc ccgcggcgac gaccagtcgc gcgagctcgg
     3061 gcgtcggatc ggcggggaag cagcgctcga gcaggccgcg gccgattcct cgaccgtggt
     3121 ggcccggcag gaccatcagc tcggtcagct gctccatctc gtcgaagcgg cagacccggg
     3181 cgtagccgac cggctcgccg gcgtcctcgc agacccagta ggtgccgccc ggctgcgcgg
     3241 cgacgaactc gaccacctcg cgttgggcgg cccactcgga ctcgatctgc gcggcggtcg
     3301 gcggcgcccc ctcgatcagc cccatccgcg tcgcggtgtg atgcacggcg a
//
LOCUS       DATABV010000229        16958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_640760, whole genome shotgun sequence.
ACCESSION   DATABV010000229 DATABV010000000
VERSION     DATABV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16958
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640760"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="VHF88_10540"
     CDS             complement(<1..1998)
                     /locus_tag="VHF88_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2162243.1"
                     /translation="MGDEEQQSSRGAKAYAGWEGRRYPPFGAKWEELLARRRRTTWGR
                     FTWISPSPLLASINDKIVKGLDDGMVNDRRARWVAEARKGAGLAGDFTVEQRLDEDEL
                     SFLVIGDTGEQDASQYATVAPIQAVGDKTDFAVIASDVIYPAGDVNDYIDGFYLPYEG
                     YRAPIYAIPGNHDWYDGLNGFMFHFCNVPPLAPIKYRAGAYSWRERVARALWRHPSPP
                     EDRDELIQQRSLRAAATGSPGPVQPGPYYAIDTKHLRIVCIDTGITGKLDREQGEWLV
                     RVSKERKCDKVLITGKPIYVNGRYEPGPIDRGAPDDSDARDPLAPFASVDDVVRHPDH
                     RYVAAIGGDVHNYQHYPVRLEQEDRGIEYLVSGGGGAYLGATHPIDRIDIDPGADGVP
                     SGVRSLNEQDVTLYPLRGDSLTRFIVRFAPMLGLALLVALVIVAGTAAAFVLPQSGFG
                     ERVGVLAPGSAHDPPLWAVMLAGPATLVGVVVLGMASRRIGGLAPFGYRTFTMIAIAA
                     LLVFLTAYAGGEQWPAEWPWIWRMAVSTLLAVVVPAAVLCGYYLLKDWIPAAVRVALA
                     CAPALAFGVVLTAGDVDGRLLAIAVLGVLVAVVVLVGAIRGKDHVPLDATPAAAAAAE
                     SAGTLAPQRAAVATFLHRLRSPAARRKLRLRAGRLLPRAIAG"
     gene            2061..2134
                     /locus_tag="VHF88_10545"
     tRNA            2061..2134
                     /locus_tag="VHF88_10545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2095..2097,aa:Arg,seq:cct)
     gene            complement(2167..3114)
                     /locus_tag="VHF88_10550"
     CDS             complement(2167..3114)
                     /locus_tag="VHF88_10550"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2162244.1"
                     /translation="MSRAKLAHQAFLYDGAEQFAATMAPLVRAGIERGDKVVVAAKRE
                     SADALRTELGTDGGDVELHDTLEWHPHPADRLTAVQQTVAQLAPGRSLLALGEPIWHG
                     SEATRREWARYESTLNVALVDAPLRFICLYDRSELPEGILRHGRGAHPELVEDGGVCP
                     CTTFERPADCVRELDAAGAVPPPPDRYDIPFRGDQHSFRGALRGLALECGMGADRAEE
                     LVLASNEVVANAVVHGDPPVTTRCWVADGDFVCEVCDGGPGVPDPFAGWTLPEPGSRS
                     GWGLALTRRICDALELTAGADESCVRLYLTLDQRLAVAA"
     gene            complement(3200..4417)
                     /locus_tag="VHF88_10555"
     CDS             complement(3200..4417)
                     /locus_tag="VHF88_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162245.1"
                     /translation="MRVLVTSARMPFALSVIRQLAQSGHEVYSSDTYESAPGNHSRYL
                     KGHFVTASPREETETFTRQVRDYCAEHRIELVVPTWEEAFYLATAREQIEQVARLYTA
                     EFETLARVHDKHSFEQLVERLGIRAPVGVTVRSDAELMEAIGRWPHYFGRGVYSRGGV
                     TLLTNHGPLAGRVKPEDVHPTPEAPWLVQEFVEGPMVCTYSTLHDGRVSAHSTYRAPR
                     QFHHSTGVQFESIDGAETLAVVEKLGAELGYSGQMSLDFVDTGDGLAIIECNPRTTDG
                     VLLMEGDELANGLLAGIDPTVPLPDEPFVVPPGRLIQLDFAVFGAMFSSGWREIPGTI
                     HDLVEVRGADRGWHDQLPNLYSFLAFCHHERLSLREHSKLQVAMYEDVSWDGEPISGM
                     TASQAAALVRLHE"
     gene            complement(4479..5051)
                     /locus_tag="VHF88_10560"
     CDS             complement(4479..5051)
                     /locus_tag="VHF88_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162246.1"
                     /translation="MPAVISGEPSKKTVLSKTETPQPLSESEKFLAGLLMLAGAVLTV
                     VVLEILLSDGKWLIEESSTTGDGATKVVESRKYSDTVPLAAVGIGGLLFLCGAFFGRL
                     REITLPGGAGIKLDDAKQHMDEKVEQAAEKAVEGKQLSEPQMAVFKETAHKEANARLF
                     SEIVLGPRPMAEWEFDRIANEAIDSAERVL"
     gene            complement(5159..5710)
                     /gene="pyrE"
                     /locus_tag="VHF88_10565"
     CDS             complement(5159..5710)
                     /gene="pyrE"
                     /locus_tag="VHF88_10565"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX2162247.1"
                     /translation="MSARERLIAELREHALVIGRVTLASGAEAEYYVDAKRATLRPAG
                     FKAMGELVAAEAERLGATAVGGLTMGADPVACAALGAGADVKAFFVRKEAKAHGLQRR
                     VEGPFLEDSDRCLVVEDVVTSGGSTIQAIEALQAEGREIVGVVSVLDRLAGGAERIVA
                     AAGGVPYTPLATIDDVYPDRPDR"
     gene            complement(5719..6750)
                     /locus_tag="VHF88_10570"
     CDS             complement(5719..6750)
                     /locus_tag="VHF88_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX2162248.1"
                     /translation="MRLPASIAAVIAAVALGAPAVASAQSSQPGQTPTVTTPAPPPPL
                     DATPPPAPKAGKAKLAVEGGIATSKLRYVARGDRLTIRGTVRPFVARQIAVLEVRRGG
                     ELVDRQRAKVRRAKRGGKVAFNFKPRRKGVYTARILHARTAGQKAFSSRVARFKAVVL
                     SAGEGRRGTDVLLLQRGLGNLGFAVPVTGYYDAGTSRAVIAFRKVNGMERIGYASPAV
                     FSMVLKGEGAFRPRFPDAGYHVEYDWSRQVLGFFRGARPVNVYHSSSGAPATPTVFGT
                     FTFYRKEPGTNSLGMVQSNYFIRGYAIHGYKDVPIYPASHGCLRVPIPNAYQIDRQIS
                     LGMKIMVYR"
     gene            complement(6747..7655)
                     /locus_tag="VHF88_10575"
     CDS             complement(6747..7655)
                     /locus_tag="VHF88_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2162249.1"
                     /translation="MEHGSPPPERLDDDPLAEPPPPLPSVPQPSWPPRFAFYGFVAAL
                     VCTLVTVGVILAIAGVGEDDESPVVTVVATLIQGFFFVGVAVAFARRIARPRPWHFGL
                     RRAPLWRTIGWAALGMLAFYILTAVYSVIVEPDVEQDVTDSLGAGEGTLGLVAAGLMV
                     IVVAPFVEELFFRGFFYRALRTRFPIALAAVVDGVVFGVIHFNFEGADALLILPPLAF
                     LGVIFCLVYERTKSLWAVIGMHAFNNTIAFSAQADDGWKVAVVAGPVMLLACCVLPQL
                     LRDGPSPLPPGAASVGPSAQLSLPIP"
     gene            complement(7660..8382)
                     /locus_tag="VHF88_10580"
     CDS             complement(7660..8382)
                     /locus_tag="VHF88_10580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2162250.1"
                     /translation="MARTQAVLLDALGTLVELDDPAPRLRDALVQRSGVDVGIAAAER
                     GFGAEIGYYLANQMRGSDQAGLERLRDDCATAMRDALLAALGESEPARPDDADLGGAL
                     DHATVRAAMLEALAFRTFPDVLPALRDLRERGLRLVVVSNWDCSLPEWLDRAGIGELV
                     DGSVSSAVIGEAKPSPAVFEAGLRLAGCDAPEALFVGDSVENDVVGARAAGLRAVLVQ
                     RGGDTPPGVQAVRSLGELSAIV"
     gene            complement(8382..9719)
                     /locus_tag="VHF88_10585"
     CDS             complement(8382..9719)
                     /locus_tag="VHF88_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX2162251.1"
                     /translation="MTREATQAAEVAGLVARVRASEAVRMAWRAFAWSRLAIFAVAVF
                     SALTAGAALERNAGKFDVPAVTGPLGGFGDLLFSPLARWDAVWFLTIANDGYDGADSA
                     RHAFFPLYPMLARVLAVFGGGGTGPMLIAAYVVSLAAFFAALVLLYRLTALELGRRAA
                     WPTLLLLCVFPASLYFGAPYSESLFLLCSVGAFYAARSGSWAWAGVAAAGASATRSAG
                     VLLLLPLVFLYLYGPRGDAAAPAPAGRRLGSRLAAFAPRYRIRADVLWLALAPAGLVA
                     YATYLGLTYGDPLSFSHVQEFWNRTFAGPLVGAWDGTAAAFDGVRQLLSGSRDVAYFE
                     QAAGDPFLVAAQNLMLFGFLCFALAGAVGVLRRLPFAYGLYVVTALMLPLSYPVAAQP
                     LMSLPRFVAVLFPFFMWLGLVCEERRIVERVAIASAIVLGLFVSQFATWQWVA"
     gene            9718..10497
                     /locus_tag="VHF88_10590"
     CDS             9718..10497
                     /locus_tag="VHF88_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2162252.1"
                     /translation="MPMPDGPMAASFVAEHRNELTAVITVLAAFVIAELVDRMLNRHV
                     HRAAKRMVRGELSPVADTRLRLVRRLVFVTILVIGIALAMAQFPSVKRVATGVLASSA
                     LLGLVVGFAARQTLANSIAGISLAISQPIRVGDLVTFEEETGEVEDIRLAYTSIRCDD
                     GRRLVVPNERLAQSSIVNHTVVDPRVKVVVDIWLPPDADTDRALDLLTHDQVEVRIAE
                     ITHDGIRLTATTWAENASARGQVASTLRQESLRRLRTDALS"
     gene            10622..12751
                     /locus_tag="VHF88_10595"
     CDS             10622..12751
                     /locus_tag="VHF88_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2162253.1"
                     /translation="MTYRQRRQRRRRATHRAKPLFVALTVLGAIAGIAATSVAGYVIA
                     VAASAPALDELKPNDKGESSVIYAADGTRLGYVQSDEIRTPIGWDELPAHLREATVAI
                     EDENFYKHAGVDYGAIVRAGIKNLESGKSLQGGSTITQQLVRALYIDDPERNIQRKIR
                     EAKLASELEQERSKKWILQNYLNDVPYGTVGGRTAIGVEAAAETFFDKHARNLTLPEA
                     ATLAGLPQAPSQYNPFHNPRAALERRNEVLGQMLDNGYITPAEHSEAVSTPLDVEQGT
                     RYTRRREPYFFDYVQEQLIERYGVGVYRRGGLKVHTTINPKMQDVARDAINAYYGDPA
                     GPSSAIVSIDPSNGEIKAMASSGTYQDRRFNLAAQAHRQPGSAFKTMVLTAAVLEGVD
                     PDATSYTSTSPITIDVPGSVPWTVKTFGGAGAGTMSLTRATLSSDNSVYAQLIMDVGP
                     DSVCKTARLLGIRTKLDCNPAEGLGGLRLGVTPLEMASAYATLAAGGVRHRPTAIKRV
                     VFPDGKSENLSNGDGKRVMTDGQAYEVTQILEMNVTSGTGTAAGYGCPAAGKTGTTDE
                     AKDAWFVGYTPKLSTSVWVGYPDANIAMPGAQGGTTAAPVWHAFMSPAAGDDCSDFPE
                     PEEPFESTPFYGKYASGGGSTSSYDDYSYSSGDSYTESYDSGGGTGDESSGAYQAPAT
                     EAPAPAPTPAPTPGGAPAPAGGGVQTP"
     gene            12916..13866
                     /gene="thiL"
                     /locus_tag="VHF88_10600"
     CDS             12916..13866
                     /gene="thiL"
                     /locus_tag="VHF88_10600"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX2162254.1"
                     /translation="MGELELIVAIRGALGEPGERVVRPSGDDAAVVRARPFAVTSIDA
                     VAEGVHFDLATCSSADAGHRALATALSDLAAMGAEPGEAYVSLALPAAFHDADALEIA
                     HGMAALADATGTTIAGGDVVRAGSLVITVAVTGWADSEDELVGRAGARPGDLVGVTGQ
                     LGGAGAGLHLLRAGASDRAGLIDRHLRPVPRLAAGRALAEAGATAMIDLSDGLATDGR
                     HLAGASSVALEIEAAALPLAPGVAEVATGAGRDPRELAIAGGEDYELLFTIPADRWDA
                     AVAAVDVPLTRLGRALDGEGLRFTGRDTADLDAVRGYEHL"
     gene            complement(14093..15868)
                     /locus_tag="VHF88_10605"
     CDS             complement(14093..15868)
                     /locus_tag="VHF88_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162255.1"
                     /translation="MIEPRIYRAAFVPALFAVVLVMFSLESRPRPLPQGLAADVLFEG
                     EQAAAEARRIASEQPDRRAGTAGDLAVADEVGDALRVSGFAVERQRFDHDGDELVNVI
                     GRRAGRSRRQVVVVAARDATGVPDAAGSAADTAALIEIARVFKGRPSRKTLILASVDG
                     SALGDVGTARLLDEVDGPDLVDGVLVVSGLGADDDERPAIVAWSNDTTRGGIALQRTA
                     AESLREELEPPGPSASPAGQVARMAFPLGIGAQGVLLAEGYDAVRISGNGQLSDGSAG
                     EPVDEDRLGALGRATLRTITALDQGPRPERGPQTYVTIAGQVMPGWVLAVLAGALILP
                     VLVAGIDAFARARRRRSPVGPWLLWIAAGLAPFAIGYGLANLLDLFGATPEPPPAPVA
                     PSLYPLDGAAIAVLAVVLAAVASSWVALRRFAGPTARRLDEASGSGAGVAVGLALGAG
                     ALVLWLIDPFAALVAVPAVHCWMLATLVDPPPARRVRFGLIAAGLVLPLLLAVYQLFV
                     LGLDPLAGAWYLLLLVLGGQVGVGSVAVAALFTAVLGSVVTIARRSGEGPGAPAGEPL
                     TPTTRGPLSYAGPGSLGGTDSALRR"
     gene            15943..16647
                     /locus_tag="VHF88_10610"
     CDS             15943..16647
                     /locus_tag="VHF88_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2162256.1"
                     /translation="MAQQAPRILLVDDEQSVQKLLASALRKDGYEVVCAPDGQAALDS
                     VAGEPFDLLVLDLMLPKVDGFEVCRQVRATSSVPIIMLTAKVEEIDKVLGLELGADDY
                     ITKPFSMREFRSRVKAVLRRSQLARAEEPGEEALEDGELRIDFDKRQVTLAGEPVRLT
                     YVEFEILAALARHPGRVYSRATLLERVWGDSAYRDPRTVDVHIRHLREKLERDPKTPE
                     LVQTVRGVGYRFRDRR"
     gene            16644..>16958
                     /locus_tag="VHF88_10615"
     CDS             16644..>16958
                     /locus_tag="VHF88_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162257.1"
                     /translation="MSARPRPRPPFGRSLRDKLTLLFFGVTAGALAVLYFLVVPELES
                     NLREQKFDALERVAEGTQDRLDELTLDPDVTSPQLDRRVAAIASAIDARVTLFGIQQA
                     LGR"
BASE COUNT         2598 a         6363 c         5856 g         2141 t
ORIGIN      
        1 gccggcgatc gcccgcggca gcagccgccc ggcgcgcagg cggagcttgc gccgcgccgc
       61 cggcgagcgc agccggtgga gaaaggtcgc aacggcggca cgctgcggcg cgagcgtccc
      121 ggcggactcg gccgccgccg ctgcggcggg ggtcgcgtcc agcggcacgt gatccttgcc
      181 gcgtatcgcc cccacgagga cgaccacggc gacgaggacg cccaggacgg cgatggcgag
      241 caagcgcccg tccacatcgc ccgcggtgag caccacgcca aacgcgagcg ccggggcgca
      301 ggcgagcgcg acacgcacgg cggccgggat ccagtccttg agcaggtagt agccgcatag
      361 gaccgccgcc ggcacgacga ccgcgagcag cgtcgacacc gccatgcgcc agatccacgg
      421 ccactcggca ggccactgct cgccgcccgc gtaggccgtc aggaagacga gcagcgcggc
      481 gatcgcgatc atcgtgaagg tgcggtagcc gaacggcgcc aggcccccga tgcggcgact
      541 ggccataccg aggacgacaa cgcccaccag tgtcgccggg ccggcgagca tcacggccca
      601 cagcggcggg tcgtgcgcgc tgcccggcgc gagcacgccg acgcgctcgc caaagccgct
      661 ctgcggcaac acaaaggcgg cagcggtccc cgcgacgatc accagcgcga ccagcagcgc
      721 gagcccgagc atcggcgcga agcggacgat gaaccgggtc agcgagtcgc cgcgcagcgg
      781 gtagagggtg acgtcctgct cgttgagcga ccgaacgccg ctcggcacgc cgtcggcacc
      841 cgggtcgatg tcgatcctgt cgatggggtg ggtggcgccg aggtaggcgc caccgccgcc
      901 gctgaccaga tactcgatgc cgcgatcctc ctgctccagc cggaccgggt agtgctggta
      961 gttgtggacg tcgccgccga tcgccgcgac gtagcggtgg tcgggatggc gcaccacgtc
     1021 gtcgaccgag gcgaacgggg caagcgggtc cctggcgtcg ctgtcgtcgg gcgccccgcg
     1081 gtcgatcggg ccgggctcgt acctgccatt tacgtagatc ggcttgccgg tgatcaggac
     1141 cttgtcgcac ttgcgctcct tcgacacccg caccagccac tcgccctgct cgcggtcgag
     1201 cttcccggtg atgccggtat cgatgcagac gatccgcagg tgcttggtgt cgatcgcgta
     1261 gtacgggccc ggctgcacgg gcccgggtga ccccgtcgcc gcggctcgca gcgagcgctg
     1321 ctggatcagc tcgtcgcggt cctccggcgg cgacgggtgg cgccagagcg cgcgggcgac
     1381 ccgctcgcgc cagctgtagg cgccggcgcg gtacttgatc ggagcgagcg gcggaacgtt
     1441 gcagaagtgg aacatgaagc cgttcagccc gtcgtaccag tcgtggttgc cggggatcgc
     1501 gtagatcggc gcccggtagc cctcgtacgg caggtagaag ccgtcgatgt agtcgttgac
     1561 gtcgcccgcc ggatagatga cgtcgctcgc gatcacggcg aagtcggtct tgtcgccgac
     1621 cgcctggata ggcgcgactg tcgcgtactg ggaggcgtcc tgctcgccgg tgtccccgat
     1681 caccaggaac gagagctcgt cctcgtcgag gcgctgctcg acggtgaagt cgccggcgag
     1741 cccggcgccc ttgcgcgcct cggccaccca gcgggcgcga cggtcgttga ccatcccgtc
     1801 gtcgagcccc ttgacgatct tgtcgttgat cgaggccagc agcgggctcg ggctgatcca
     1861 ggtgaaccgc ccccaggtcg tgcgacggcg gcgggccagc agctcctccc atttggcccc
     1921 gaagggcgga tagcgccgcc cctcccatcc agcgtaggcc ttcgccccgc gcgagctctg
     1981 ctgctcctcg tcccccatgt ccctcctccc tgagccggtc gcccgaccct aacccacgcc
     2041 gtgcgatcat gggcgcagag gccccgatag ctcagccgga tagagcgtcc ccctcctaag
     2101 gggaaggccg ctggttcgaa tccagctcgg ggcactgccg cggcgccgag ccggagctgg
     2161 cgcggctcag gccgccacgg ccagccgctg atcgagcgtg aggtagagcc gtacacacga
     2221 ctcgtcggcg cctgccgtca gctcgagcgc gtcgcagatc cgtcgcgtca gcgcgaggcc
     2281 ccagccgctg cgcgagccgg gctcgggcag ggtccagccg gcgaacgggt ccgggacgcc
     2341 gggaccgccg tcgcagacct cgcagacgaa gtcgccatcg gcgacccagc accgcgtcgt
     2401 caccggcggg tcgccatgga ccacggcgtt ggcgaccacc tcgttggacg ccagcacgag
     2461 ctcctccgcg cggtcggcgc ccatgccgca ctccagcgcc agcccccgca acgctcccct
     2521 gaagctgtgc tggtcgccgc ggaaggggat gtcgtagcgg tccggggggg gcggcacggc
     2581 gccggcggcg tcgagctccc ggacgcagtc ggcgggccgc tcgaacgtcg tgcagggaca
     2641 gacgccgccg tcctcgacga gctcggggtg ggcgccgcga ccgtgacgca ggatgccttc
     2701 cggcaactcc gagcggtcat agaggcagat gaatcgcagc ggcgcgtcca cgagcgccac
     2761 gttgagggtg gactcgtagc gggcccactc gcgccgagtc gcctcagagc cgtgccagat
     2821 cggctcgccg agcgcgagca ggctcctgcc cggtgcgagc tgagcgaccg tctgctgaac
     2881 cgccgtcagg cggtcggccg ggtgggggtg ccactcgagc gtgtcgtgga gctcgacgtc
     2941 cccgccgtcg gtcccgagtt cggtgcgcag tgcgtcagcg ctctcgcgct tggcggccac
     3001 gaccaccttg tcgccccgtt ctatccccgc gcgcacgagg ggcgccatcg tcgccgcaaa
     3061 ctgctcggcg ccgtcataca ggaaggcctg gtgggccagc ttggcccggc tcatcgggtc
     3121 gatcgcggcg gtatcaccag accccccata cccagccgcg ccgagggccg ctccaaccaa
     3181 tcggccaaca gagggcgcgc tactcgtgca atcgcacaag cgctgcggcc tgggaggcgg
     3241 tcatgccgct gatcggctcg ccatcccatg agacgtcctc gtacatcgcg acctggagct
     3301 tgctgtgctc gcgcaggctc agccgctcgt ggtggcagaa ggcgaggaac gagtagaggt
     3361 tgggcagctg gtcgtgccag ccgcggtcgg cgccgcgcac ctcgacgagg tcgtggatcg
     3421 tcccggggat ctcgcgccag ccgctcgaga acatcgctcc gaagacggcg aagtcgagct
     3481 ggatcaggcg gccgggcggg acgacgaacg gctcgtcggg caggggcacc gtcgggtcga
     3541 tcccagccag cagcccgttc gccagctcgt cgccctccat cagcagcacg ccatcggtcg
     3601 tgcgcgggtt gcactcgatg atcgccaggc cgtcgccggt gtcgacgaag tcgagcgaca
     3661 tctggccgct gtagccgagc tccgcgccga gcttctcgac cactgcgagc gtctccgccc
     3721 cgtcgatcga ctcgaactgg acgccggtgc tgtgatggaa ctggcgcggc gcgcggtagg
     3781 tcgaatgggc gctcacgcgg ccgtcgtgga gggtgctgta cgtgcagacc atcgggccct
     3841 cgacgaactc ctgcaccagc cagggcgcct ccggcgtcgg gtgcacgtcc tcgggcttca
     3901 cccggccggc gagtggcccg tggttggtca gcagcgtcac cccaccgcgc gagtagacgc
     3961 cgcggccgaa gtagtggggc cagcggccga tcgcctccat cagctcggcg tcgctgcgca
     4021 ccgtcacccc gaccggcgcc cggatcccga gccgctcgac gagctgctcg aacgagtgct
     4081 tgtcgtggac ccgcgcgagc gtctcgaact ccgccgtgta gagccgcgcc acctgctcga
     4141 tctgctcgcg cgcggtcgcg aggtagaagg cctcctccca ggtcggcacc acgagctcga
     4201 tccgatgctc ggcgcagtag tcgcgcacct gacgcgtgaa cgtctcggtc tcctcgcgcg
     4261 gcgaggccgt gacgaagtgg cccttcagat agcgcgagtg gttgcccggc gccgactcgt
     4321 atgtgtccga ggagtagacc tcgtgcccgg actgcgcgag ctggcggatc acgctcagcg
     4381 cgaacggcat ccgcgcgctc gtgacgagga ccctcacgcg ggcaccgtag cccctagggg
     4441 cgtcgaacgg gttcccgccc aggcccgggg ccggatcgct agagaacgcg ctccgccgag
     4501 tcgatcgcct cgttggcgat gcgatcgaac tcccactccg ccatcggtcg tgggccgagg
     4561 acgatctcgc tgaagaggcg ggcgttcgcc tccttgtgcg cggtctcctt gaagaccgcc
     4621 atctggggct cggacagctg cttcccctcc acggccttct cggcagcctg ctcgactttc
     4681 tcatccatgt gctgcttggc gtcgtcgagc ttgatcccgg cgccacccgg cagcgtgatc
     4741 tcgcgcagac ggccgaagaa cgcgccgcag aggaacagca ggccgccgat ccccaccgcc
     4801 gcgagtggca ccgtgtcgga gtacttcctg ctctcgacca ccttcgtcgc accgtcgccg
     4861 gtcgtgctcg actcctcgat cagccacttg ccgtccgaca gcaggatctc cagcacgacg
     4921 acggtgagca cggcgcccgc cagcatcagc agcccagcaa ggaacttctc gctttctgac
     4981 agcggctgag gcgtctcagt cttggagaga accgtcttct tggacggctc tccggaaatg
     5041 acagcaggca ctcgaactcc ctcctcgcct tggaccgaac ccgcgccatc cttacctatt
     5101 cgacattaac tgtcaataca cggagatcgc acgactgcga gctttacggt aaccgcagct
     5161 agcggtcggg gcggtccgga tacacgtcgt cgatcgtcgc gagcggggtg tagggcacgc
     5221 cgccggccgc ggcgacgatc cgctcagcac cgcccgccag ccggtcgagc acgctgacca
     5281 ccccgacgat ctcacggccc tctgcctgca gcgcctcgat cgcctggatc gtcgagccgc
     5341 cgctcgtgac cacgtcctcg acgacgaggc agcggtccga gtcctcgagg aacggcccct
     5401 cgacgcgccg ctgcagcccg tgcgccttgg cctccttgcg gacgaagaac gccttgacgt
     5461 ccgcgcccgc gccgagcgcc gcgcaggcga cgggatcagc ccccatcgtc agcccgccga
     5521 ccgccgtcgc gccgagccgc tcggcctccg ccgcgaccag ctcgcccatc gccttgaagc
     5581 cggccggccg caaggtcgcc cgctttgcgt caacgtagta ctccgcctcg gcgccgctgg
     5641 cgagcgtcac cctcccgatc accagcgcgt gctcgcgcag ctcggcgatc aggcgttcgc
     5701 gggcactcat cgcgagcgct agcggtagac catgatcttc atccccaacg agatctggcg
     5761 atcgatctgg tacgcgttgg ggatcggcac ccgcaggcag ccgtggctcg ccgggtagat
     5821 cgggacgtcc ttgtagccgt ggatcgcgta gccgcggatg aagtagttcg actggaccat
     5881 cccgagcgag ttggtgcccg gctccttgcg gtagaaggtg aacgtcccga agacggtcgg
     5941 cgtcgccggc gcgcctgacg acgagtggta gacgttgacc ggccgcgcgc cccggaagaa
     6001 gccgagcacc tgacgcgacc agtcgtactc gacgtggtag ccggcgtcgg ggaacctcgg
     6061 cctgaaggcg ccctcgccct tgagcaccat cgagaagacg gctgggctgg cgtagccgat
     6121 ccgctccatc ccgttgacct tgcggaaggc gatcaccgcg cgcgacgtgc cggcgtcgta
     6181 gtagccggtg accggcaccg cgaagccgag gttgccgagc ccgcgctgga gcagcagcac
     6241 gtcggtgccg cggcggccct cgccggcgct cagcacgacc gccttgaagc gcgcgacgcg
     6301 tgagctgaat gccttctgac cggccgtcct ggcgtgcagg atgcgcgccg tgtagacgcc
     6361 cttgcggcgc ggcttgaagt tgaacgcgac cttgccgccg cgcttggcgc ggcgcacctt
     6421 cgcgcgctgg cggtcgacca gctcgccgcc gcggcgcacc tcgagcaccg cgatctggcg
     6481 ggcgacgaag ggacgcaccg tgccgcggat cgtcagccgg tcgccgcgcg cgacgtagcg
     6541 cagcttgctc gtcgcgatcc cgccctcgac cgcgagcttg gccttgcccg ccttcggcgc
     6601 cggcggcggt gtcgcgtcga gcggcggggg cggagccggc gtggtgacgg tcggcgtctg
     6661 accgggttgc gaggactggg cggaggcgac ggccggggcg ccgagggcga cggcggcgat
     6721 cacggcggcg atggaggcgg ggagtctcat gggatcggca acgataactg cgcgctggga
     6781 ccgacagagg ccgcgccggg cggcagcggc gacggcccgt cgcgcaggag ctgcggcagg
     6841 acgcaacacg cgagcaacat gaccgggccg gcgacgaccg ccaccttcca gccgtcgtcg
     6901 gcctgcgccg agaaggcgat cgtgttgttg aacgcgtgca tcccgatcac cgcccagagc
     6961 gacttggtgc gctcgtagac gaggcagaag atgacaccga ggaaggcgag cggcggcagg
     7021 atcagcagcg cgtcggcgcc ctcgaagttg aagtggatga ccccgaagac gacgccgtcg
     7081 acgacggccg ccagcgcgat cgggaagcgg gtgcgcagcg cgcggtagaa gaagccgcgg
     7141 aagaacagct cctcgacgaa cggcgcgacg acgatcacca tcagtccggc ggcgaccagg
     7201 ccgagcgtgc cctcgccggc gccgaggctg tcggtcacgt cctgctcgac gtcgggctcg
     7261 acgatcaccg agtagaccgc cgtcaggatg tagaaggcga gcatcccgag cgccgcccag
     7321 ccgatcgtgc gccagagcgg cgcgcgacgc aggccgaagt gccacggacg cggccgcgcg
     7381 atcctgcgcg cgaacgccac ggcgacgcca acgaagaaga agccctggat cagcgtcgcg
     7441 acgacggtca cgaccggcga ctcgtcgtcc tctccgacgc cggcgatggc caggatcacc
     7501 ccgacggtca cgagcgtgca gacgagcgcc gcgacgaaac cgtagaaggc gaaccgcggc
     7561 ggccacgacg gctgcggcac gcttgggagc ggcggcgggg gctcggcgag cgggtcgtcg
     7621 tcgagccgct cgggcggcgg ggaaccgtgc tccatcagct cagactatgg cggacagctc
     7681 gccgagcgac cgtaccgcct ggacccccgg cggcgtgtcg ccgccgcgct ggacgagcac
     7741 ggcgcgcaac ccggcggccc gcgcgccaac gacgtcgttc tcgaccgagt cgccgacgaa
     7801 cagtgcctcc ggcgcgtcgc aaccggctag gcgcaacccg gcctcgaaga cagcaggcga
     7861 cggcttggcc tcgccgatga ctgccgacga caccgacccg tcgacgagct cgccgatccc
     7921 ggcgcggtcg agccactcgg gcagcgagca gtcccagttg ctgacgacga ccagccgcag
     7981 cccgcgctcg cgcagatctc gcagcgccgg cagcacgtcg ggaaaggtcc ggaacgccag
     8041 cgcctccagc atcgccgcgc gcacggtcgc atggtcgagc gcgccgccca ggtccgcgtc
     8101 gtccggccgc gccggctcgc tctcgccgag cgccgccagc agcgcgtcgc gcatcgccgt
     8161 cgcgcagtcg tcgcgcagcc gctcgaggcc cgcctggtcg ctgccgcgca tctggttcgc
     8221 gaggtagtag ccgatctcag cgccgaagcc gcgctccgcc gccgcgatcc cgacgtcgac
     8281 gccgctgcgc tgcaccagcg cgtcgcgcag ccgcggcgcg ggatcgtcga gctcgaccag
     8341 cgtgccgagc gcgtcgagca ggaccgcctg cgtccgcgcc atcacgccac ccactgccag
     8401 gtcgcgaact ggctgacgaa cagcccgagc acgatcgccg acgcgatcgc gacccgctcg
     8461 acgatccgcc gctcctcgca gacgagcccg agccacatga agaacgggaa cagcaccgcc
     8521 acgaagcgcg gcagcgacat cagcggctgt gcggccaccg gataggagag cggcagcatc
     8581 agcgccgtca cgacatagag gccgtaggcg aacggcagcc gtcgcagcac cccgacggct
     8641 ccagcgagcg cgaagcagag gaagccgaac agcatcaggt tctgcgccgc gactaggaac
     8701 ggatcgcccg ccgcctgctc gaagtaggcg acgtcgcggc tgccagagag cagctggcgc
     8761 acgccgtcga acgccgccgc cgtgccgtcc caggcaccga ccagcggccc cgcgaacgtc
     8821 cggttccaga actcctggac gtgggagaac gacagcgggt cgccgtaggt gaggccgagg
     8881 taggtcgcgt aggcgaccag cccggccggc gccagcgcga gccagaggac gtccgcccgg
     8941 atccggtagc gcggcgcgaa cgccgccagc cggctgccca ggcgccgccc cgccggcgct
     9001 ggcgccgcgg cgtcgccgcg cgggccatag aggtagagga acaccagcgg cagaagcagc
     9061 agaaccccgg cgctgcgcgt cgcggacgct cccgccgccg ccaccccggc ccatgcccag
     9121 ctgccgctgc gcgccgcata gaacgccccg accgagcaga gcaggaacag gctctccgag
     9181 tacggcgcgc cgaagtacag cgacgccggg aagacgcaga ggagcagcag cgtcggccac
     9241 gccgcgcgcc gccccagctc gagtgccgtc agccggtaca gcagcaccag cgccgcaaag
     9301 aacgccgcca gcgacacgac gtaggcggcg atcagcatcg gcccggtccc gcccccaccg
     9361 aagaccgcca gcacgcgcgc cagcatcggg tagagcggga agaaggcgtg tcgcgccgaa
     9421 tcggcgccgt cgtagccgtc gttcgcgatc gtcaggaacc agaccgcgtc ccagcgcgcc
     9481 agcggcgaga acagcaggtc gccgaagccg ccgagcggcc ccgtcaccgc cggtacgtcg
     9541 aacttgcccg cgttccgctc gagcgcggcg cccgcggtca gcgccgagaa gaccgccacc
     9601 gcgaagatcg ccaggcgcga ccacgcgaac gcccgccacg ccatccgcac cgcctcgctc
     9661 gcgcgcacgc gcgcgacgag ccccgcgacc tccgctgcct gagtagcctc ccgcgtcatg
     9721 ccgatgccgg atggtcccat ggcggcgtcg ttcgtcgcgg agcaccgcaa cgagctgacc
     9781 gccgtcatca ccgtgctcgc cgcgttcgtg atcgccgagc tcgtcgaccg gatgctgaac
     9841 cgccacgtcc accgcgcagc gaagcggatg gtccgcggcg agctctcgcc cgtcgccgac
     9901 acccgcctcc ggctcgtccg ccgcctcgtc ttcgtgacga tcctcgtgat cggcatcgcc
     9961 ctcgcgatgg cccagttccc ctccgtcaag cgcgtcgcca ccggcgtcct cgcctcctcc
    10021 gccctgctcg gcctcgtcgt cggcttcgcc gcccgccaga ccctcgccaa ctcgatcgcc
    10081 ggcatctcgc tcgccatctc ccagccgatc cgcgtcggcg acctcgtcac cttcgaggag
    10141 gagaccggcg aggtcgagga catccgcctc gcctacacct cgatccgctg cgacgacggc
    10201 cgccgcctcg tcgtccccaa cgagcgtctc gcccaatcct cgatcgtcaa ccacaccgtc
    10261 gtcgaccccc gcgtcaaagt cgtagtcgac atctggctcc ccccagacgc cgacacagac
    10321 cgcgccctcg acctcctcac acacgaccaa gtcgaggtcc gcatcgcgga gatcacccac
    10381 gacggaatcc gcctcacagc aaccacctgg gccgagaacg catccgcccg aggccaagtc
    10441 gcctccaccc tcaggcagga aagccttcgc cgcttgagaa ccgacgctct atcctgaagc
    10501 agttcaccga cgccacatcc gcgggtggcg acgattttgc gtttgcgcgc gcagcttgcg
    10561 agcacgtaaa ccaaaatcgg agacaggacc cgcggccacc aacgcccggg cccgccaccc
    10621 catgacctac cgccaacgcc gccagcgccg ccgccgcgcc acccaccggg ccaagcccct
    10681 cttcgtcgcc ctcaccgtcc tcggcgccat cgccggcatc gccgccacct ccgtcgccgg
    10741 ctacgtcatc gccgtcgccg cctccgcccc cgccctcgac gagctcaagc ccaacgacaa
    10801 gggcgagtcg agcgtcatct acgccgccga cggcacccgc ctcggctacg tccagtccga
    10861 cgagatccgc acgccgatcg gctgggacga gctgcccgcc cacctgcgcg aggcgaccgt
    10921 cgcgatcgag gacgagaact tctacaagca cgccggcgtc gactacggcg cgatcgtccg
    10981 cgcggggatc aagaacctcg agtccggcaa gagcctgcag ggcggctcga cgatcaccca
    11041 gcagctggtg cgcgcgctct acatcgacga ccccgagcgc aacatccagc gcaagatccg
    11101 cgaggcgaag ctggcgagcg agctcgagca ggagcgctcg aagaagtgga tcctgcagaa
    11161 ctacctcaac gacgtgccgt acggcacggt cggtgggcgg acggcgatcg gcgtcgaggc
    11221 ggccgccgag accttcttcg acaagcatgc gcgcaacctg accctgccgg aggcggcgac
    11281 gctcgccggc ctgccgcagg cgccgtcgca gtacaacccg ttccacaacc cgcgcgcggc
    11341 gctcgagcgg cgcaacgagg tgctcgggca gatgctcgac aacggctaca tcacgccggc
    11401 ggagcacagc gaggcggtgt ccaccccgct cgacgtcgag caggggaccc gctacacgcg
    11461 ccgccgcgag ccgtacttct tcgactacgt ccaggagcag ctgatcgagc gctacggcgt
    11521 cggggtctac cgccgcggcg gcctgaaggt ccacacgacg atcaacccga agatgcagga
    11581 cgtcgcgcgc gacgcgatca acgcctacta cggcgacccg gcggggccgt cgtcggcgat
    11641 cgtctcgatc gacccgtcga acggcgagat caaggcgatg gcgtcgagcg gcacgtacca
    11701 ggaccgccgc ttcaacctgg cggcgcaggc gcaccgccag cccggctcgg cgttcaagac
    11761 gatggtgctg accgcggcgg tgctcgaggg cgtcgacccg gacgcgacga gctacacgtc
    11821 gacgagcccg atcacgatcg acgtgccggg ctccgtgccg tggaccgtca agaccttcgg
    11881 cggcgccggc gccggcacca tgagcctgac gcgcgcgacg ctgtcctcgg acaactcggt
    11941 ctacgcgcag ctgatcatgg acgtcggccc agactcggtc tgcaagacgg cgcggctact
    12001 cggtatccgg acgaagctcg actgcaaccc tgccgagggc ctcggcggcc tgcggctcgg
    12061 cgtcaccccg ctcgagatgg cgagcgccta cgcgacgctg gcggcgggcg gcgtgcgcca
    12121 ccggccgacg gcgatcaagc gcgtcgtctt ccccgacggc aagagcgaga acctctccaa
    12181 cggcgacggc aagcgcgtga tgaccgacgg ccaggcctac gaggtgacgc agatcctcga
    12241 gatgaacgtc acgtcaggca ccggcaccgc cgccggctac ggctgcccgg ccgccggcaa
    12301 gacgggaacg accgacgagg cgaaggacgc ctggttcgtc ggctacacgc cgaagctctc
    12361 gacgtcggtc tgggtcggct acccggacgc gaacatcgcg atgccgggcg cccagggcgg
    12421 cacgaccgcg gcgccggtct ggcacgcctt catgtcgccc gccgcgggcg acgactgcag
    12481 cgacttcccc gagccggagg agccgttcga gtcgacgccg ttctacggca agtacgcgag
    12541 cggcggcggc tcgaccagct cctacgacga ctactcgtac tcgagcggcg actcctacac
    12601 cgagagctac gacagcggtg gcggcaccgg cgacgagagc tccggggcct accaggcgcc
    12661 ggcgaccgag gcccccgccc cggcgccgac ccccgccccg acgcccggcg gtgcgcccgc
    12721 gcccgctggc ggcggcgtcc agacgccgta gatggctcac ctcgcggctc ggccgctcgg
    12781 cgcccgcccg atgagggatt cgcatcgcgg gaacggctcg atccttcact cgccactggc
    12841 acgtgggacc gcggtcgcct cgctgagggg tctgcatcgc ctggacagca cgatccctca
    12901 tcggggcctg gacgcgtggg cgagctcgag ctgatcgtgg cgatacgggg agcgctcggc
    12961 gagcctgggg agcgggtggt tcggccgagc ggcgacgacg ccgcggtcgt gcgcgcccgg
    13021 ccgttcgccg tcacctcgat cgacgccgtc gccgagggcg tccacttcga cctcgcgacc
    13081 tgctcgtccg ccgacgccgg ccaccgcgcc ctcgcaacgg cgctgtcgga cctcgccgcg
    13141 atgggcgccg agcccggcga ggcctacgtc tcgctcgcgc tgccggcggc cttccacgac
    13201 gccgacgccc tggagatcgc gcacgggatg gcggcattgg ccgacgcgac cggcacgacg
    13261 atcgccggcg gcgacgtcgt ccgcgccggc tcgctcgtca tcacggtcgc cgtgactggc
    13321 tgggccgact ccgaggacga gctggtcggc cgcgccggcg cgcgccccgg cgacctggtc
    13381 ggagtgaccg gccaactcgg cggggcaggc gccggcctcc atctgcttcg cgccggcgcg
    13441 agcgatcgcg cggggctgat cgaccggcac cttcgtccgg tgccccggct tgccgccggt
    13501 cgggcgcttg cggaggccgg tgcgacggcg atgatcgatc tcagcgacgg gctcgccacc
    13561 gacggtcgcc acctcgcggg tgcgagcagc gtggcgctcg agatcgaggc tgccgccctg
    13621 ccgctcgctc cgggcgtcgc cgaggttgcc accggggccg gccgcgatcc gcgcgagctc
    13681 gcgatcgcgg gcggcgagga ctacgagctg ctgttcacga tccccgccga ccgctgggac
    13741 gctgcggtcg ccgcggtcga cgtcccgctg acccggctag gccgtgcgct ggacggggaa
    13801 gggctgcggt tcacgggccg cgatacggcg gacctcgatg cggtccgggg ctacgagcac
    13861 ctgtgagcag cgcgacccgc ggcgcgcgac gcgcagcgcg tgcgcacggg tgagcatcgc
    13921 ctgcgcgacc gcgttggtgt cgaccgtgta ctcgccattg gcgagcctgc tgcgcaggtc
    13981 ctggctcatg tggctctgat cggccgcctc cattccgatg ctgagattgt ggtggtcgcg
    14041 gtggacgctt ggtcggcgcc agcgcccggc caggcggccc gatcccgcac cgtcagcggc
    14101 gcagcgcgct gtcagtgccg ccaagcgatc ccgggcccgc gtagctcagc ggcccgcgcg
    14161 tggtcggcgt caacggctcg ccggccgggg ctcccggccc ctcgccggag cggcgggcga
    14221 tcgtgacgac cgatccgagc acggccgtga acaacgccgc gaccgccacc gagcccactc
    14281 ctacctggcc gccgaggacc agcagcagca ggtaccaggc gccggcgagc gggtccaggc
    14341 cgagcacgaa cagctggtag accgccagca gcagcggcag gaccagcccg gccgcgatca
    14401 ggccgaagcg cacacgccgc gccggcggcg ggtcgaccag cgtggccagc atccagcagt
    14461 ggaccgccgg gaccgcgacc agggcggcaa aggggtcgat cagccacagg acgagcgcac
    14521 ccgcgccgag cgccaggccg accgcgacgc ccgcgccgga gccggacgcc tcgtcgagcc
    14581 tgcgcgccgt cggcccggcg aagcgccgca gtgcgaccca gctcgacgca acagccgcga
    14641 ggacgaccgc gagcacagcg atcgccgccc cgtcgagcgg gtagaggctc ggcgcgaccg
    14701 gggccggcgg cggctcgggc gtggcgccga acaggtcgag caggttggcg aggccgtagc
    14761 cgatcgcgaa cggcgccagg ccggcggcga tccagagcag ccacggcccg accggcgagc
    14821 gacggcggcg ggcgcgcgcg aaggcgtcga tgccggccac cagcaccggc aggatcaagg
    14881 ccccggcgag gacggcaagc acccagcccg gcatcacctg gccggcgatc gtcacgtagg
    14941 tctgcggacc gcgctccggt cgcgggccct ggtcgagcgc cgtgatcgtg cgcagcgtcg
    15001 cgcgaccgag cgcgccgagg cggtcctcgt cgaccggctc gccggcgctg ccatcggaca
    15061 gctggccgtt gccggatatc cgcacggcgt cgtagccctc ggcgagcagc acaccctgcg
    15121 cgccgatgcc gagcgggaac gccatccgcg cgacctggcc ggccggactc gccgaaggac
    15181 cgggcggctc gagctcctcg cgcagcgact cggcggccgt ccgctggagc gcgatcccgc
    15241 cgcgcgtcgt gtcgttgctc cacgcgacga tcgccggacg ctcgtcgtca tcggcgccga
    15301 gcccggaaac gacgagcacg ccgtcgacga ggtccggccc gtcgacctcg tcgagcagcc
    15361 gcgccgtccc gacgtccccg agcgccgacc cgtccaccga cgccaggatc agcgtcttgc
    15421 gcgacggccg ccccttgaag acgcgcgcga tctcgatcag cgcggctgtg tcggcggcgc
    15481 tgcccgcggc gtccgggacg ccggtcgcgt cgcgtgccgc gaccaccaca acctgcctgc
    15541 gcgagcggcc ggcgcggcgt ccgatcacgt tgaccagctc gtcgccgtcg tggtcgaagc
    15601 gctgacgctc gacggcgaag ccgctcacac gcagcgcgtc gccgacctcg tccgcgaccg
    15661 ccaggtcgcc cgccgttccg gcgcggcggt ccggttgctc gctggcgatc cggcgcgcct
    15721 cggcggccgc ctgctcgccc tcgaacagca cgtcggccgc caacccctgc ggcagcggcc
    15781 gcggccggct ctcgagcgag aacatcacga gcacgaccgc gaacagcgca ggcacgaacg
    15841 ccgctcggta gatccgaggc tcgatcatgg ccgttccctc acgtccgacc caacctacaa
    15901 tctcgcccag gcgccggtgc ctcgcggtac ctcgtcactc cgatggctca gcaggctccc
    15961 cgcatcctcc tggtcgacga cgagcagtcg gtccagaagc tgctcgcgtc agctctgcgc
    16021 aaggacggct acgaggtcgt ctgcgcgccc gacggacagg cggcactcga cagcgtcgcc
    16081 ggcgagccgt tcgacctgct cgtgctcgac ctgatgctgc cgaaggtcga cggcttcgag
    16141 gtctgccgcc aggtgcgcgc gaccagctcg gtgccgatca tcatgctgac ggcgaaggtc
    16201 gaggagatcg acaaggtgct cgggctcgag ctcggcgccg acgactacat caccaagccg
    16261 ttctcgatgc gcgagttccg cagccgcgtg aaggcggtac tgcgccgctc ccagctcgcg
    16321 cgcgccgagg agccgggcga ggaggcgctc gaagacggcg agctgcgaat cgacttcgac
    16381 aagcgccagg tgacgctcgc cggcgagccg gtgcggctga cctacgtcga gttcgagatc
    16441 ctcgccgcgc tcgcccgcca tccgggccgc gtctacagcc gtgcgacgct gctcgagcgc
    16501 gtctggggcg actccgccta ccgcgacccg cgcaccgtcg acgtccacat ccgccacctg
    16561 cgcgagaagc tcgagcgcga ccccaagacg cccgagctgg tccagaccgt gcgcggcgtc
    16621 ggctaccgct tccgcgaccg ccgatgagcg cgcgaccgcg cccgcgaccg ccattcgggc
    16681 gctcactgcg cgacaagctg acgctgctgt tcttcggcgt aaccgcgggc gccttggccg
    16741 tcctctactt cctcgtcgtc cccgagctcg agtccaacct gcgcgagcag aagttcgacg
    16801 ccctggagcg cgtcgccgag ggcacccagg accgcctcga cgagctgacc ctcgaccccg
    16861 atgtaacctc gccgcagctc gaccgccgcg tcgccgcgat cgccagcgcg atcgacgcgc
    16921 gcgtgaccct gttcggcatc cagcaggcgc tcggccgc
//
LOCUS       DATABV010000230        17010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_559665, whole genome shotgun sequence.
ACCESSION   DATABV010000230 DATABV010000000
VERSION     DATABV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17010
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559665"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1524)
                     /locus_tag="VHF88_10620"
     CDS             complement(19..1524)
                     /locus_tag="VHF88_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2162258.1"
                     /translation="MLAAVVGLHPTRLQGGRHDGSTVTTVERPFNKCYNFATIELAVN
                     NPATEPSGDDAPLVPDPLPPGPSGPGPLVFANFLARPGGFLESSKRRYGTPFTFKMSP
                     RRTVVITDDPAVIKQVFTSDPTKLLAGVGNEVLRPLLGPRSVLTLDEPEHMRQRKLLL
                     PPFHGDRMKLYGEVIAEATEREMARWPVGEPFAVQQSMQAITLEVIMRAVFGVRDDRE
                     RMEQIAEPLRRLLDSMADMRRLFTLQVSSSKRNGPLSPWRHFRRTLLHPADEVLYEEI
                     RTHRADADVGERDDILSLLLSARDEDGEGLTDSELHDELMTLLLAGHETTATALSWTL
                     ERLVRHPEALARLTEEARDGEGGEYMDAVIKESLRLRPVVPAVARYLTEPTELGGRVL
                     PAGVHITPSIYLTHRNPAIYDEPDRFRPERFLERPAGTYEWIPFGGGTRRCLGASFAL
                     FEMRIVLGGVLRRFDVVSAGERDERIARRAITFSPARGARVRLARRPPKAI"
     gene            1471..2115
                     /locus_tag="VHF88_10625"
     CDS             1471..2115
                     /locus_tag="VHF88_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2162259.1"
                     /translation="MTTTLQPRRMKTDDRREHLLRAGAELLGRRPYEEVSIDEIARAA
                     GISKGLLYHYFPTKKDFVVAVLRQEVDRVTALTAPDPSLPAVEQIDAALDAFLDYVEH
                     HASAYAAIFRTRGGGDPEIRAVLEEGRERRVEIVLDGVAGQLLAAGADRESAVLRSAV
                     QGWISFVEGVLLRWLDKRDLERDQLRELLRAVLIDSLRAAVHIDPRLRLESDRL"
     gene            2308..2820
                     /locus_tag="VHF88_10630"
     CDS             2308..2820
                     /locus_tag="VHF88_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162260.1"
                     /translation="MPPDELSGPDLAGVVADANAARLPYVVIGGFSVIYHGFVRATKD
                     SDLLIPDGPDADAAVVRFLDRTRAVRLHDGKRVGFEDVAGQHHLRMKTRHGIVDIMRG
                     GLPPLDFETVAARAVEDEWEGESVRVAALSSVVGFKRLAGRLQDQVDLEQLQAIYGEL
                     PIEPIPGVDS"
     gene            complement(2827..3537)
                     /locus_tag="VHF88_10635"
     CDS             complement(2827..3537)
                     /locus_tag="VHF88_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX2162261.1"
                     /translation="MNAPGDTRPLIRVGHKGADHVAPGNTAESFEAALELGVDMIEFD
                     VMPLPDGTLVLAHDPRDAESRTPLTLEEGLEHFAAEAYADVELDVDLKIPGYEAAVVE
                     GLRERGLLERSLISSTYPESLRLVRQLDPHVRRGWSVPRVRRDLRRHPVLKYVAYGAV
                     AWWRAGLPTKAERALRRGECEALMSHYVLVTRQLVQRVKDAGGQLYVWTVDDAAEIAR
                     LEALGVDAVITNDPRLFG"
     gene            complement(3598..4455)
                     /locus_tag="VHF88_10640"
     CDS             complement(3598..4455)
                     /locus_tag="VHF88_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2162262.1"
                     /translation="MPEGDTIHNAALRVGNALVGHEILEIETPHPRYRMDRWPERLAG
                     RAVRAVDARGKHLFLRFEGDLTIHSHLRMGGKWGVYERGRRWTRGAHRAWLVIRTAGH
                     DVVQFDGPVLELMTDSRSRFDQLLAGLGPDIVADDFDERLYLARLREDDQTRAIGEAL
                     LDQRNVAGIGNIWKSEGCFMALVDPWRRTADLTDEELLAIARAIRPEIRRSARQGGPT
                     VTYRAETASAGAPARRPQRRPVDRDRRAGTWVYERTGLPCRVCGTPVLARGQGDDNRT
                     TYWCVVCQS"
     gene            4549..4764
                     /locus_tag="VHF88_10645"
     CDS             4549..4764
                     /locus_tag="VHF88_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162263.1"
                     /translation="MKFEESPVTAFLEAAATSVGVGMVLGGFFAGLAGVLCGAPREDL
                     ERQVLTAGYLVAALCFALRLVELARII"
     gene            4783..4977
                     /locus_tag="VHF88_10650"
     CDS             4783..4977
                     /locus_tag="VHF88_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162264.1"
                     /translation="MSLRNETIGFVVGIGILIVGVLLTSGDPDQLEGALIALLMVGVP
                     LWLSYYLHLRDGSYPRSSRR"
     gene            complement(4988..9112)
                     /locus_tag="VHF88_10655"
     CDS             complement(4988..9112)
                     /locus_tag="VHF88_10655"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX2162265.1"
                     /translation="MRVEDMREPGAHDIGHPAVETVGTAAGGGDLMGHGREPGTEALP
                     SLDPQVGVEAPYADPLAGTGFGDDPNAATRRSIWPAIYPELLELVQSHTSTIIFVNAR
                     RAAERLAVRLNELAGEDGQPADIARAHHGSLAREERLVVEDMLKSGSLPCLVATSSLE
                     LGIDMGAVDLVLQVESPKSVTAGLQRIGRAGHNVGDVSKGRIFPKFRADLLECAVVAK
                     RMRAAEIETTVIPRNPLDVLAQQIVAMVSSPPVDGGPSAPREAASAADGDAATTRAAD
                     TEVGGEEWPVPLLYDLVRRAHPYSDLPRELLNQVLDMLDGRYPSEEFAELRPRIVWDR
                     VADTIRARKGARQLAITNAGTIPDRGLYGVHLPDGRRVGELDEEMVYEARPGQTFLLG
                     ASTWRIEEITRDRVIVTPAPGAPGAIPFWKGDGIGRPAELGRAIGEFARWAVDREPAE
                     LIDAGYDLDERAAKNLIVYLREQQEATRVIPSDRTIVVERFRDEIGDWRLCVLSPFGG
                     RVHSAWALAISARIRDEFGLESDAIWSDDGIIVHLPDADEPPGSELVLVEPDAVEDLV
                     VGELSASALYGARFRENAARALLLPRAYPGKRTPLWQQRLKAQSLLEVAKRYGQFPIV
                     LETYRECLRDVLDLPGLEQLLTGLHTRELSLVEVETQRASPFASSLLFDYVATYMYEG
                     DAPNAERRAAALSLDRDLLRELLGQEELRELIDAGALAEVEADLQHLSRRTQAANADA
                     LHDVLRGVGDLTLAEAALRCLPTGASARPAADREAVAGEAGSDGTGSDDGRATGGATV
                     DEAEALLAVLARERRAIQMRIGGEERWIASEDAGLYRDAFGAVPPGGLPDAFLESVEA
                     PFERLVRRWARTHGPFTTRELKDRYVVDPTAVLKALEGAGNLVRGELRPGGSEREWCD
                     PEVLRRLRRASLASLRKEVEPAEQQALARFLPAWQGVDASPASGAGPDRLREMLVPLQ
                     GLALAPAVWEQDVLPRRTGAYSPAWLDQLCAGGEVVWVGAGPLGRNTGKVALYFRDDA
                     SWLGPPPFKGDAPAGPVHDAIRARLANGAAFWTDLIADVHVTGASGDGNGASAAGGRL
                     APDPAELQEALWDLVWAGEVTNDAFAPLRAPRLSLANARRESQRRFARRRRPGAPQVQ
                     GRWSLAANLFRDPPAQGPRLRAQAELLLERYGVVTRETVLAEGIPGGFSSLYGELANL
                     ETLGTARRGYFVEGLGGAQFALPAAIERLRGLRTDTRPGTGEPAGPLVLAATDPANPF
                     GATLPWPKRDDNRRPARVPGAHVVTLDANPVLYVERSGKGLIALRDPLEPNGTPAPWL
                     ADALQALADQVRRGRIKRLALERFDGDPVVGSPFEPLLVEIGFSQGPRRLTLSA"
     gene            complement(9119..9943)
                     /locus_tag="VHF88_10660"
     CDS             complement(9119..9943)
                     /locus_tag="VHF88_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2162266.1"
                     /translation="MPSDPLASFTPQVREWFARSFEAPTDAQTQAWPAIAGGEHVLVS
                     APTGSGKTLAAFLWGLDRLVANPLPRREEHSRTGHVAGDVAARPASTAPTPSGERRTR
                     LVYVSPLKALSYDVEKNLRGPLQGIHAGAETDGVGPDGGAARGKITVAIRTGDTPQRE
                     RQAMLRTPPDVLITTPESLYLMLTSRAREFLTGVEWAIVDEIHAVAQTKRGAHLALTL
                     ERLDHLTRGGDPAAPGIQRIGLSATQRPLEEVGRFLVGTGRECRIVDTGVRKPLDL"
     gene            complement(10043..10651)
                     /locus_tag="VHF88_10665"
     CDS             complement(10043..10651)
                     /locus_tag="VHF88_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162267.1"
                     /translation="MSNALIRRVDAIAATFSLALLATVALAAVAPSADARTLPGKAPA
                     KAKTTVGGGAVAGTPLSKLTAPGAKAKLTRNGLAIPPAGAPAKVQAVIRAGNEIAKKP
                     YKWGGGHGLLVDSGYDCSGSVSYALRKARLMTSSLASTGFMSWGRRGRGDWITIRANA
                     GHAYMVVAGLRFDTSASKRTGTRWSAQMRSPRGYVATHPPGF"
     regulatory      complement(10661..10818)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(10931..11602)
                     /locus_tag="VHF88_10670"
     CDS             complement(10931..11602)
                     /locus_tag="VHF88_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2162268.1"
                     /translation="MADSLDELERRVTCCRRCPRLVEWRELVAREKRAAFRDEEYWGR
                     PAPGFGDPAARVYILGLAPAAHGANRTGRIFTGDRSGDWLFASLYRMGFANQPESVDR
                     DDGLRLTDAFIAAAVRCAPPANKPLPSERDNCLPYAAEELELMTSVRVIVALGGFAWD
                     AALRLHGPVRPRPKFGHAAETPLPGGRTLIGCYHPSQQNTFTGRLTEEMTDAVLVRAR
                     QLAQR"
     gene            complement(11622..12386)
                     /locus_tag="VHF88_10675"
     CDS             complement(11622..12386)
                     /locus_tag="VHF88_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2162269.1"
                     /translation="MNGYRSMGWLARREVLRVSKLWTQTVLASVVSSFLFILVFGLSL
                     GGRIKQVEGFDYEVFIVPGLIAMAMVQAAYANNASTIYQGRSDRFIHDVLSAPMHGWQ
                     MTAGYIIGGLFRGFAIAVALLALALPITGVPVEQPLVLVLATALALLGFASLGTVVGI
                     YAESWDHTTFIQNIVILPLTFMGGVFYSVDMLSSPWEELSHFNPIFYMLQAIRYGFLG
                     SADVSIWLSLGVTAALAIPCYLWAQWLFTSGRKLKA"
     gene            complement(12383..13123)
                     /locus_tag="VHF88_10680"
     CDS             complement(12383..13123)
                     /locus_tag="VHF88_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2162270.1"
                     /translation="MTELAPPALEVRGLTKRYDDGTLAVEGFDMQVPDGAFFGLLGPN
                     GAGKTTLISAVCNLIRVTDGEILVFGEPAKSLQARSWIGLAEQDINLDRFLSVRETLI
                     YHGGYFGMTGDEAEARADEMIDAFDLRAKSGVRTPKLSGGQRRRLLLARALMHRPRLV
                     ILDEPTAGVDFELRLELWRYIRQLHAEGTTILLTTHYLEEAEELCEEIALIRGGHLIA
                     RDSADGLREHYGAGTLADVYVKAMGAGA"
     gene            complement(13134..14126)
                     /gene="rfbB"
                     /locus_tag="VHF88_10685"
     CDS             complement(13134..14126)
                     /gene="rfbB"
                     /locus_tag="VHF88_10685"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX2162271.1"
                     /translation="MKLLVTGAAGFIGSTYARIATDAGHELTILDKLTYAGRRENLEG
                     VEHRFVHGGIEDPEKVREAMEGADAVVNFAAESHVDRSIAGQDPFVTTHVIGTGVLLD
                     AARELGVNRYLQVSTDEVYGSIESGSFTETSPLNPSSPYSATKAAGDLLVSAHVHTHG
                     IEAVICRGSNNYGPRQYPEKLIPLMTLNALHGDSLPVYGDGRQVRNWLYVEDFARAIH
                     LVLERGAPGEAYNVGGPDECENIDVVRRIIELTGKDESLMEYVTDRPGHDRRYSLASE
                     KVRALGWEARVHFDKGLERTVAWYRDNRDWWEPIRSGEYREYYERQYGRALSGK"
     gene            complement(14209..15048)
                     /gene="rfbD"
                     /locus_tag="VHF88_10690"
     CDS             complement(14209..15048)
                     /gene="rfbD"
                     /locus_tag="VHF88_10690"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX2162272.1"
                     /translation="MKVLITGAGGMLGQDVRRAAEAGNHEVDALDRAALDVTDAGAVS
                     DAFAQLRPDAAINCAAWTDVDGAETAEEEALRLNADAAGNVAAAAASVGASVVQPSTD
                     YVFDGTSTRPYVESDPTGPQSAYGRTKLAGEQAVAAANPRHFVVRTAWLFGVGGRNFV
                     ETMLGLAGGGVVTVVRDQVGSPTYTAHLAEGLLRLAATPAYGVHHMAAGGQCSWYDFA
                     RAIFEDAGVECQVLSTTTAEFARPAPRPAYSVLDTEWPDPIRLPDWRDGLRAYLRERG
                     GAA"
     gene            15489..16049
                     /locus_tag="VHF88_10695"
     CDS             15489..16049
                     /locus_tag="VHF88_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162273.1"
                     /translation="MKIQKRVIELHRAFDRHEIPHAFGGALALAYWTLDPRGTNDIDV
                     NLFMPAADCDRALRSLPDGVEQPEGTVDRIRRDGQIRLWWEEIPVDLFFDYAPVHEDA
                     ARDRQVVPFLDIDIPVLGPVELAVFKAMFDRTKDWADVEAMIAAQRLDVDLVRESLRT
                     MLASDDHRFARLDEAERLAATASPPG"
     gene            16074..>17010
                     /locus_tag="VHF88_10700"
     CDS             16074..>17010
                     /locus_tag="VHF88_10700"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX2162274.1"
                     /translation="MRRGLIAVAVALFALPSGAAAQTAEPLAAQLEPPLPSPVVAGDW
                     PDPDVSLIDGSYYAVATSGGWSPTFRVLQSTDLRSWSIAGSVFGRPPGWTKDSFWAPE
                     LAPLPAGGYALFYSAMPKRPWQTRADGSTVPYRGARVRPGGPQVPRAGRPWYCLGVAT
                     APSPVGPWRDLGRPLRCTPRGTIDPTPVVDGDKLYLVYKEDGNAFRKPTPILLQELRA
                     DGRRLLGAPRELLRNQPRTWERNVIEAPFLVRRDGGWTMLYSGALCCSPDCAYAVGAA
                     RAPTLAGPWTRYPGNPILRSGNGWRCPGHVSVVGDN"
BASE COUNT         2372 a         5908 c         6249 g         2481 t
ORIGIN      
        1 ctgccggcgc cccagcggtc agatcgcctt gggtgggcgg cgcgcgagcc gcacgcgtgc
       61 cccccgggcg gggctgaacg tgatcgcccg gcgggctatc cgctcgtcgc gctcacccgc
      121 ggacacaacg tcgaaccgcc gcagcacgcc gccgagcacg atcctcatct cgaacagcgc
      181 gaagctcgcg ccgaggcagc ggcgcgtacc gcctccgaac ggaatccact cgtaggtgcc
      241 cgccgggcgc tccaggaagc gctcgggacg gaagcggtcg ggctcgtcat agatggccgg
      301 gttgcggtgg gtgaggtaga tcgacggggt gatgtggacg ccggccggga ggacgcggcc
      361 gccgagctcg gtcggctcgg tcaggtagcg cgccaccgcc ggcacgaccg gccgcaggcg
      421 cagcgactcc ttgatcaccg cgtccatgta ctcgccgccc tcgccgtcgc gcgcctcctc
      481 ggtcagtcgt gcgagcgcct cgggatggcg caccagccgc tccagcgtcc acgacagcgc
      541 cgtcgccgtc gtctcgtgac ccgccagcag cagcgtcatc agctcgtcgt gcagctcgga
      601 gtcggtcagg ccctccccgt cctcgtctcg ggccgacagc agcagcgaca ggatgtcgtc
      661 gcgctcgccg acgtcggcgt cggcgcggtg cgtccggatc tcctcgtaga gcacctcgtc
      721 cgcggggtgc agcagcgtcc tacggaagtg gcgccacggg ctcagcgggc cgttgcgctt
      781 cgagctcgac acctgcagcg tgaacagccg ccgcatgtcg gccatcgagt cgagcagccg
      841 ccgcagcggc tcggcgatct gctccatccg ctcgcgatcg tcgcgcacgc cgaagaccgc
      901 gcgcatgatc acctcgagcg tgatcgcctg catcgactgc tggaccgcga acggctcgcc
      961 gaccggccag cgcgccatct cgcgctcggt cgcctcggcg atcacctcgc cgtagagctt
     1021 catccggtcg ccgtggaagg gcggcagcag cagcttgcgc tggcgcatgt gctccggctc
     1081 gtcgagcgtc agcaccgacc gcgggccgag cagcggccgc agcacctcgt tgccgacgcc
     1141 ggccagcagc ttggtcgggt cgctggtgaa gacctgcttg atcaccgccg ggtcgtcggt
     1201 gatcacgacg gtgcgccggg gcgacatctt gaaagtgaac ggggtgccgt agcgccgctt
     1261 ggagctctcg aggaagccgc ccggccgcgc gaggaagttc gcgaacacga gcgggcccgg
     1321 tccgctgggg ccgggcggca gcgggtccgg gacgagtgga gcgtcgtcgc cggagggctc
     1381 ggtggcgggg ttgttgaccg ctagttcaat agtggcgaag ttgtagcact tgttgaacgg
     1441 ccgttcaaca gtcgttaccg tggaaccgtc atgacgacca ccttgcagcc gcgtcggatg
     1501 aagaccgacg accgccgcga gcacctcctg cgggccgggg ccgagctgct cggacgacgg
     1561 ccctacgagg aggtctcgat cgacgagatc gcgcgcgcgg cggggatctc gaagggcctc
     1621 ctgtaccact acttcccgac caagaaggac ttcgtcgtcg ccgtcctgcg ccaggaggtc
     1681 gaccgcgtga ccgctctcac cgcccccgac ccctcgctgc cggcggtcga acagatcgac
     1741 gccgcgctcg acgccttcct cgactacgtc gagcaccacg cctccgccta cgcggcgatc
     1801 ttccgcaccc gcggcggcgg tgaccccgag atccgggccg tgctagagga ggggcgcgag
     1861 cggcgcgtcg agatcgtgct cgacggcgtc gccggccagc tgctcgccgc cggcgccgac
     1921 cgcgagtccg ccgtcctgcg ctcggccgtc cagggctgga tctcgttcgt cgagggagtc
     1981 ctgctgcgct ggctcgacaa gcgcgacctc gagcgtgacc agctgcggga gctgctgcgc
     2041 gccgtgctga tcgactcttt gcgcgcggcg gtccacatcg acccccggct ccggctcgaa
     2101 tcggatagac tataagcatg gctgctgctc gtacccgacc tcttctcgcg cccggcaacg
     2161 aggacgagtg ggctgagatc aatcggacag aggcaaagca gctccgcgtc agcaacatgg
     2221 cgctcacgcc gacggagcga ctcagtctgg ggcagcagat gtcccagcag gcggtctcgc
     2281 tgctctcggc ttcggtgagg gccgggcatg cccccagacg agctctctgg tcctgacctc
     2341 gccggcgtcg tagccgacgc caacgccgcc aggctgccct acgtcgtgat cggcggcttc
     2401 agcgtgatct accacgggtt cgtgcgcgcg acgaaggaca gcgacctgct gattcccgat
     2461 gggccggacg ccgacgcggc ggtcgtccgc ttcctcgacc ggacgcgggc ggtccggctt
     2521 cacgacggga agcgggtggg cttcgaggac gtggcggggc agcaccacct tcgcatgaag
     2581 acgcgccacg gaatcgtcga catcatgcgc ggcgggttgc cgccactcga cttcgagacc
     2641 gtcgccgcgc gagccgtcga ggacgagtgg gaaggcgagt cggtccgcgt ggccgcgctg
     2701 tcgagcgtcg tgggcttcaa gcggctcgcc ggacgcctgc aggatcaggt cgacctcgag
     2761 cagctgcagg ccatctacgg cgagctgccg atcgagccga tccccggcgt cgacagctga
     2821 cgtcgatcag ccgaacaacc gcgggtcgtt ggtgatgacc gcgtccaccc cgagcgcctc
     2881 gagccgcgcg atctccgcgg cgtcgtcaac ggtccagacg tagagctggc cgccggcatc
     2941 cttcacccgc tggacgagct ggcgggtgac gagcacgtag tgcgacatca gcgcctcgca
     3001 ctcgccgcgg cgaagcgccc gctcggcctt ggtggggagg ccggcgcgcc accaggcgac
     3061 cgccccgtaa gcaacgtact tgagcaccgg atgcctgcgg aggtcgcggc ggacgcgcgg
     3121 gaccgaccag ccgcggcgga cgtgggggtc gagctgacgc accagccgca ggctctcggg
     3181 ataggtggac gagatcagcg agcgctctag caggccgcgc tcgcgcaggc cctcgacgac
     3241 cgccgcctcg tagccgggga tcttgagatc gacgtcgagc tcgacgtcgg cgtaggcctc
     3301 ggcggcgaag tgctcgagcc cctcctcgag cgtcagcggc gtgcggctct ccgcgtcccg
     3361 gggatcgtgg gccagcacga gcgtcccgtc gggcagcggc atcacgtcga actcgatcat
     3421 gtcgaccccg agctcgagcg ccgcctcgaa gctctcggcg gtgttgccgg gcgcgacgtg
     3481 gtcggccccc ttgtggccga cgcggatcag cgggcgggtg tcgccgggag cgttcatcgg
     3541 ggtggatcgt aggggggcgg cgctcgatgt cgccacgggc tgttcgtagc ctgctggtca
     3601 gctctggcag accacgcacc agtaggtcgt gcggttgtcg tcgccctggc cgcgggcgag
     3661 gaccggcgtg ccgcagaccc ggcacggcag gccggtccgc tcgtagaccc acgtcccggc
     3721 gcggcggtcg cggtcgacag ggcggcgttg cggccggcgc gcgggcgcgc cggccgacgc
     3781 ggtctcggcg cggtaggtga cggtcggccc gccctggcgc gccgagcgac ggatctccgg
     3841 tcggatcgcg cgcgcgatcg cgagcagctc ctcgtcggtc agatcggcgg tgcggcgcca
     3901 ggggtccacg agcgccatga agcagccctc cgacttccag atgttgccga ttccggcgac
     3961 gttgcgctgg tcgagcagcg cctcgccgat cgcccgtgtc tggtcgtcct cgcgcaggcg
     4021 ggcgagatag agccgctcgt cgaagtcgtc ggcgacgatg tcggggccga ggcccgccag
     4081 cagctggtcg aagcggctgc gcgagtcggt catcagctcg agcaccgggc cgtcgaactg
     4141 gacgacgtcg tggccagcgg tacggatgac cagccaagcg cgatgagccc cgcgcgtcca
     4201 ccgccgcccg cgctcgtaca cgccccactt cccacccatt cgcaggtggg agtggatcgt
     4261 caggtcgcct tcgaagcgaa ggaacaggtg cttgccgcgg gcgtcgaccg cgcggacggc
     4321 gcgcccggct agccgctcgg gccagcggtc catccggtac cgcggatgcg gcgtctcgat
     4381 ctcgaggatc tcgtgtccga cgagcgcgtt cccgacccgc agcgccgcat tgtggatcgt
     4441 gtcgccctcg ggcatcttct cgatcgtagg cgtcgcgttg cgcgcctcgg tttcacttgt
     4501 cgccaaagcg ctgtcacctg tgagtccagg tcgcgcaaga ttgggatcgt gaagttcgaa
     4561 gagagcccag tcacggcctt cttggaggcg gccgctacct cggtcggtgt cggcatggtg
     4621 ctgggaggtt tctttgctgg tttggccgga gtgctctgcg gggcgccgcg tgaggacctc
     4681 gagcgccaag tgttaaccgc cggttacctg gtcgccgcgt tgtgcttcgc cctgcggctg
     4741 gtcgagctag cgcgaatcat ataagctgcc ggaagatttg ctatgtcact caggaacgag
     4801 accatcggat tcgtagtcgg gatcgggatc ttgatcgtcg gcgtgcttct gacgagcggt
     4861 gatcccgacc agctcgaggg cgcgctgatc gcattgctga tggtaggtgt cccgctctgg
     4921 ctgagctact acctccatct gcgtgacggc agctatcccc gctcgtcacg tcgctagggc
     4981 caggacgtca agcgctcagg gtcagacgac gcggtccttg gctgaagccg atctcgacga
     5041 gcagcggctc gaagggagag ccgacgacgg ggtcgccgtc gaagcgctcg agggcgaggc
     5101 gcttgatgcg gccgcgacgg acctggtcgg cgagggcttg gagggcgtcg gcgagccagg
     5161 gagcgggcgt gccgttgggc tcgagcgggt cgcggagggc gatcaggccc ttgccggagc
     5221 gctcgacgta gaggacgggg ttggcgtcga gggtgacgac gtgggcgccg ggcacgcgcg
     5281 cggggcggcg gttgtcgtcg cgcttgggcc agggaagggt ggcgccgaag gggttggcgg
     5341 ggtcggtggc ggcgaggacg agcgggccgg cgggctcgcc tgtgcccggc cgggtatcgg
     5401 tccgcaggcc acgcagtcgc tcgatcgcgg cgggcagcgc gaactgggcg ccgccgagtc
     5461 cctcgacgaa gtagccgcgg cgggcggtgc cgagcgtctc gaggttggcg agctcgccgt
     5521 agagggagga gaagccgccg gggatgccct cggcgagcac ggtctcgcgg gtgacgaccc
     5581 cgtagcgctc gagcagcagc tcggcctggg cgcgcaggcg tgggccttgg gccgggggat
     5641 cgcggaagag gttggcggcc agcgaccagc gtccctgaac ctgcggcgcg cccgggcgac
     5701 ggcggcgggc gaaacggcgc tgcgactcgc ggcgggcgtt ggcgagcgac aaccggggtg
     5761 cgcgcagcgg cgcgaaggcg tcgttggtga cctcgccggc ccagacgaga tcccagagcg
     5821 cctcttggag ctcggcgggg tcgggcgcca gcctgccgcc ggccgcagac gcgccgtttc
     5881 cgtcgccgct ggcgccggtc acgtgaacgt cagcgatcag gtcggtccag aacgcggcgc
     5941 cgttcgccag ccgcgcgcgg atcgcgtcgt ggaccgggcc ggccggtgcg tcgcccttga
     6001 acggcggcgg gccaagccag gaggcgtcgt cgcggaagta cagcgcgacc ttgcccgtat
     6061 tgcggccgag cgggccggcg cccacccaga cgacctcgcc gccggcgcag agctggtcga
     6121 gccaagcggg cgagtaggcg ccggtgcggc gcggcaggac gtcctgctcc cagacggcgg
     6181 gcgcgagcgc gaggccctgg agcgggacga gcatctcgcg caggcggtcg gggccggcgc
     6241 ccgaggcggg cgatgcgtcg acgccctgcc acgccgggag gaagcgggcg agcgcctgct
     6301 gctcggccgg ctcgacctcc ttgcgcaggc tcgccagcga cgcgcggcgg aggcggcgca
     6361 gcacctcggg gtcgcaccac tcgcgctcgc tgccgccggg acgcagctcg ccgcggacga
     6421 gattgcccgc gccctcgagc gccttcagca cggccgtcgg gtcgacgacg tagcggtcct
     6481 tcagctcgcg cgtggtgaac gggccgtggg tgcgcgccca gcggcggacc agccgctcga
     6541 acggcgcctc gacgctctcg aggaaggcgt cggggaggcc gcctggtggc acagcgccga
     6601 aggcatcgcg atagaggccg gcgtcctcgg aggcgatcca gcgctcctcg ccgccgatcc
     6661 gcatctggat cgcgcggcgc tcgcgggcga gcaccgcgag cagcgcctcg gcttcgtcga
     6721 ccgtcgcgcc gccggtcgcg cggccgtcgt cgctccccgt cccgtcggac ccggcctcgc
     6781 cggccactgc ctcgcgatcg gccgcaggcc gggcactcgc tccggtcggc aggcagcgga
     6841 gcgcggcctc ggcgagcgtc aggtcgccga cgccacgcag cacgtcgtgg agcgcgtcgg
     6901 cgttcgccgc ctgcgttcgg cggctgaggt gctgcaggtc agcctcgacc tcggcgagcg
     6961 cgccggcgtc gatcagctcg cgcagctcct cctggccgag cagctcgcgc agcaggtcgc
     7021 ggtcgagcga cagcgcggcc gcccgccgct cggcgttcgg cgcgtcgccc tcgtacatgt
     7081 aggtcgcgac gtagtcgaac agcagcgacg aggcgaacgg ggaggcgcgc tgggtctcga
     7141 cctcgaccag cgacagctcg cgggtgtgga ggccggtcag cagctgctcc agccccggca
     7201 ggtcgagcac gtcgcgcagg cactcgcggt aggtctcgag gacgatcggg aactggccgt
     7261 agcgcttggc gacctcgagc agcgactgcg ccttgagacg ctgctgccag agtggcgtgc
     7321 gcttgccggg gtaggcgcgc ggcagcagca gcgcgcgggc ggcgttctcg cggaagcggg
     7381 cgccgtagag ggcggaggcc gacagctcgc cgacgacgag gtcctcgacg gcgtcgggct
     7441 cgaccagcac gagctcggac ccgggcggct cgtcggcgtc ggggaggtgg acgatgatgc
     7501 cgtcgtcaga ccagatcgcg tcagactcga ggccgaactc gtcgcgaatc cgggcggaga
     7561 tcgcaagcgc ccaggccgag tggacgcggc cgccgaacgg cgacaggacg cagaggcgcc
     7621 agtcgccgat ctcgtcgcgg aagcgctcga cgacgatcgt gcggtcggag gggattacgc
     7681 gcgtcgcctc ctgctgctcg cgcaggtaga caatcaggtt cttggcggcc cgctcgtcga
     7741 ggtcgtagcc ggcgtcgatc agctcggcgg gctcgcggtc gaccgcccag cgcgcgaact
     7801 cgccgatcgc gcgtccgagc tccgcggggc ggccgatccc gtcgcccttc cagaacggga
     7861 tcgccccggg cgcgccgggc gcgggggtga cgatcacgcg gtcgcgggtg atctcctcga
     7921 tccgccaggt cgacgcgccg agcaggaacg tctggccggg gcgcgcctcg tagaccatct
     7981 cctcgtccag ctcgccgacg cggcggccgt cgggcaagtg gacgccgtag aggccgcggt
     8041 cggggatcgt gccggcgttg gtgatcgcga gctggcgggc gcccttgcgc gcgcggatcg
     8101 tgtcggcgac gcggtcccag acgatgcgcg gccgcagctc ggcgaactcc tccgacgggt
     8161 agcggccgtc gagcatgtcg agcacctggt tcaggagctc gcgcgggagg tcggagtagg
     8221 ggtgggcgcg gcggacgagg tcgtagagga gcgggaccgg ccactcctcg ccgccgacct
     8281 cggtgtcggc ggcgcgtgta gtcgcggcgt cgccatcggc ggcggacgcc gcctcacgcg
     8341 gcgcgctcgg accgccgtcg accggaggcg acgacaccat cgcgacgatc tgctgcgcca
     8401 gcacgtcgag cgggttgcgc gggatgaccg tcgtctcgat ctccgccgct cgcatccgct
     8461 tcgcgacgac cgcgcactcg agcaggtcgg cgcggaactt ggggaagatc cgccccttcg
     8521 agacgtcgcc gacattgtgg cccgcgcgcc cgatccgctg caggccggcg gtgaccgact
     8581 tcggcgactc gacctgcagc acgaggtcga ccgcacccat gtcgatcccg agctcgagcg
     8641 acgacgtcgc gacgaggcag ggcagcgagc ccgacttgag catgtcctcg acgaccagcc
     8701 gctcctcgcg cgcgagcgag ccgtggtggg cgcgggcgat gtcggccggc tgaccgtctt
     8761 cccctgccag ctcgttcagg cggaccgcca gccgctcggc cgcgcggcgg gcattgacga
     8821 agatgatcgt cgaggtgtgg gactgcacca gctcgagcag ctcgggatag atcgccggcc
     8881 agatcgagcg gcgggtcgcg gcgttgggat cgtcgccaaa gcccgtgccc gcgagcgggt
     8941 cggcgtaggg cgcctcgacg ccaacctggg ggtcgagtga gggcagcgcc tccgtgccgg
     9001 gctcgcgtcc gtgccccatc aggtccccgc cccccgcggc cgttcctacc gtctcgaccg
     9061 ctggatggcc gatgtcgtgc gcccccggct cgcgcatgtc ctcgacccgc acttggatct
     9121 acaggtcgag cggcttgcgc acgccggtgt cgacgatcct gcactcgcgc ccggtcccga
     9181 cgaggaagcg gccgacctcc tcgagcgggc gctgggtcgc cgacaggccg atccgctgga
     9241 tgccgggcgc ggccgggtcg ccgccgcgcg tgaggtggtc gagccgctcc agcgtcaggg
     9301 cgagatgcgc gccgcgcttg gtctgggcga ccgcatggat ctcgtcgacg atcgcccact
     9361 cgacgccggt caggaactcg cgcgcccgcg aggtcagcat caggtagagc gactccggcg
     9421 tggtaatcag cacgtcgggc ggcgtgcgca gcatcgcctg gcgctcacgc tgcggcgtgt
     9481 cgccggtgcg gatcgcgacg gtgatcttcc cgcgtgcggc cccaccgtca ggtcccacgc
     9541 cgtcggtctc cgcccccgca tgaatcccct gcagtggtcc gcgcaggttc ttctcgacgt
     9601 cgtaggagag cgccttcagc ggcgagacgt agacgaggcg ggtgcgccgc tcaccggatg
     9661 gagtcggtgc ggtgctcgcc ggccgagccg ccacgtcgcc tgccacgtga ccggtccggc
     9721 tgtgctcctc ccgccggggc aacgggtttg cgacgagccg gtcgagtccc cagaggaacg
     9781 ccgcgagggt cttgcccgag ccggtcgggg cggagacaag gacgtgctcg ccaccggcga
     9841 tcgctggcca ggcctgggtc tgggcgtcgg tcggcgcctc gaacgagcgc gcgaaccact
     9901 cccttacctg gggcgtgaac gacgcgagcg ggtccgaagg catggcgcct tcgagcgtac
     9961 cgcccggcgc cggtcgcctt gcaacggcag atgcgggatt cgcatcgcct ggactaccta
    10021 atccgacact cagcgccccc gactagaagc ccggcgggtg cgtggcgacg tagccgcgcg
    10081 gcgagcgcat ctgggcgctc cagcgcgtgc cggtgcgctt gctggcgctg gtgtcgaagc
    10141 gcagtcccgc gacgaccatg taggcgtggc cggcgttggc ccggatcgtg atccagtcgc
    10201 cgcgcccgcg gcggccccat gacatgaagc cggtcgaggc tagcgaggat gtcatcaggc
    10261 gagccttgcg cagggcgtag ctgaccgagc ccgagcagtc gtagccggag tcgacgagca
    10321 gcccgtggcc gccgccccac ttgtagggct tcttggcgat ctcgttgccg gcgcggatca
    10381 ccgcctgaac cttggcgggc gcgccggcgg gcgggatcgc gagcccgttg cgcgtcagct
    10441 tcgccttcgc gccgggcgcg gtcagcttgc tgagcggcgt gccggcgacg gcgccgccgc
    10501 ccacggtcgt cttcgccttc gcgggcgcct tgccgggcag cgtacgggcg tctgcgcttg
    10561 gcgcgaccgc agcaagggcg acggtcgcca ggagggccag ggagaacgtc gcggcaatcg
    10621 catcgacgcg acgaatcagg gcgttggaca tcggggagcg tcttcctctc tttcgcgcct
    10681 gcggggttag ctgacgggct cgcgctaaga gagctgcgct acgccgggac ttccggcgat
    10741 tcgccccagg gccagggacc caagtggttc ccccgctctc ccaccgcatg gcggcgagag
    10801 actcggctat gtgctctgga ccagtcttcg acgccgtagg gcgccgacct gttcggacca
    10861 gagcaccgta ggcgatcgaa agttgcgcac tgtgacgagg ctcacagttg tgccagtcag
    10921 cccgaggcgc ctagcgttgc gccagctggc gcgcgcggac gaggacggcg tccgtcatct
    10981 cctccgtcag tcgccccgtg aaagtgttct gctggctcgg gtggtagcag ccgatcagcg
    11041 tgcggccacc cggcaacggc gtctcggccg cgtggccgaa cttcgggcgc gggcggaccg
    11101 gtccatgcaa ccgcagtgcc gcgtcccacg caaagccgcc gagcgcgacg atcacgcgca
    11161 ccgacgtcat cagctccagc tcctcggcgg catacggcag gcagttgtcg cgctcggacg
    11221 gcagcggctt gttcgccggc ggcgcgcagc ggacggcggc ggcgatgaac gcgtcggtca
    11281 gccgcaggcc gtcgtcgcga tcgacggact ccggctggtt ggcgaaaccc atccggtaga
    11341 gcgaggcgaa cagccagtcg cccgagcggt cgccggtgaa gatgcgtccg gtgcggttcg
    11401 cgccgtgcgc cgccggcgcg aggccgagga tgtacacgcg tgccgccgga tccccgaacc
    11461 ctggagctgg ccgtccccag tactcctcgt ctcggaacgc ggcgcgcttc tcgcgtgcga
    11521 cgagctcgcg ccactcgacc agccgcgggc agcggcgaca gcaagtgacg cggcgctcca
    11581 gctcgtcgag cgagtcggcc aaggctacgg atcgcgcccg gctaggcctt cagcttgcgc
    11641 ccgctggtga acagccactg cgcccagagg tagcagggga tcgcgagcgc ggcggtcact
    11701 ccgagcgaca gccagatcga gacgtcggcg ctgccgagga acccgtagcg gatcgcctgc
    11761 agcatgtaga agatcgggtt gaagtgcgac agctcctccc acggcgagga gagcatgtcg
    11821 accgagtaga agacgccgcc catgaacgtc agcggcagga tcacgatgtt ctggatgaac
    11881 gtcgtgtggt cccagctctc ggcgtagatg ccgacgaccg tgccgagcga cgcgaagccg
    11941 agcagcgcca gggcggtggc gagcacgagc acgagcggct gctcgaccgg gacgccggtg
    12001 atcggcaggg ccagcgcgag cagcgcgacc gcgatcgcga agccgcggaa cagcccgccg
    12061 atgatgtagc cggccgtcat ctgccagccg tgcatcggcg ccgacagcac gtcgtggatg
    12121 aagcgatcgg agcggccctg gtagatcgtc gacgcgttgt tggcgtaggc ggcctggacc
    12181 atcgccatcg cgatcagccc cggcacgatg aagacctcgt agtcgaagcc ctcgacctgc
    12241 ttgatccgcc cgccgagcga cagcccgaag acgaggatga acaggaacga cgagacgacg
    12301 gatgcgagca cggtctgcgt ccagagcttc gacacgcgca gcacctcgcg ccgcgccagc
    12361 catcccatgc tgcggtagcc gttcacgcgc cggcccccat cgccttgacg tagacgtcgg
    12421 cgagcgtgcc ggcgccgtag tgctcgcgca gcccgtcggc gctgtcgcgc gcaatcaggt
    12481 ggccgccgcg gatcagcgcg atctcctcgc agagctcctc ggcctcctcg aggtagtgcg
    12541 tcgtcagcag gatcgtcgtc ccctcggcat gcagctggcg gatgtagcgc cacagctcga
    12601 gccgcagctc gaagtcgacg ccggcggtcg gctcgtcgag gatcaccagc cgcgggcggt
    12661 gcatcagcgc gcgggcgagc agcagccggc ggcgctggcc gccggacagc ttcggcgtac
    12721 gcacgccgct cttggcgcgc aggtcgaagg cgtcgatcat ctcgtcggcg cgcgcctcgg
    12781 cctcgtcgcc ggtcatgccg aagtagccgc cgtggtagat cagcgtctcg cggacgctca
    12841 ggaagcggtc gaggttgatg tcctgctcgg ccagcccgat ccagctgcgc gcctgcagcg
    12901 acttcgccgg ctcgccgaaa acgaggatct ccccgtcggt cacgcggatc aggttgcaga
    12961 ccgcgctgat cagcgtcgtc ttgccggcgc cgttgggccc gagcaggccg aagaaggcgc
    13021 cgtctggcac ctgcatgtcg aagccctcga cggcgagcgt gccgtcgtcg tagcgcttgg
    13081 tcaggccgcg cacctcgagc gcgggcgggg cgagctcagt cacccaggag acgttacttg
    13141 ccgctgagcg cgcggccgta ctggcgctcg tagtactcgc ggtactcgcc ggagcggatc
    13201 ggctcccacc agtcgcggtt gtcgcggtac caggcgacgg tgcgctccag gcccttgtcg
    13261 aagtggaccc gcgcctccca gccgagcgcg cgcaccttct ccgaggcgag cgagtagcgg
    13321 cggtcgtggc cgggccggtc ggtgacgtac tccatcagcg actcgtcctt gccggtcagc
    13381 tcgatgatcc gccgcacgac gtcgatgttc tcgcactcgt cggggccgcc gacgttgtaa
    13441 gcctcgccgg gggcgccgcg ctcgagcacg aggtggatcg cgcgggcgaa gtcctcgacg
    13501 tagagccagt tgcgcacctg gcggccgtcg ccgtagacgg ggagcgagtc gccatgcagc
    13561 gcgttgagcg tcatcagcgg gatcagcttc tccgggtact ggcgcgggcc gtagttgttc
    13621 gagccgcggc agatcaccgc ctcgatgccg tgcgtgtgga cgtgggcgga gacgagcagg
    13681 tcgccggcgg ccttggtcgc gctgtagggc gacgaggggt tgagcggcga ggtctcggtg
    13741 aacgagccgg actcgatcga gccgtagacc tcgtcggtcg agacctggag gtaacggttg
    13801 acgcccagtt cgcgcgcagc gtcgagcagt acgccggtgc cgatcacgtg cgtggtgacg
    13861 aacgggtcct ggccggcgat cgagcggtcg acgtgcgact cggcggcgaa gttgacgacg
    13921 gcgtcggcgc cttccatcgc ctcgcggacc ttctccgggt cctcgatgcc gccgtgcacg
    13981 aagcggtgct cgacgccctc gaggttctcg cgccggccgg cgtaggtcag cttgtcgagg
    14041 atcgtcagct catggccggc gtcggtcgcg atccgcgcgt aggtcgatcc gatgaatccg
    14101 gcggcgccgg tgacgagcag cttcatggcc ggcgacccta tcgaggccgc tccggcgacg
    14161 ccggccacat tccggcggga gttttgccgg gatcggcccg gcgtggggtc aggcggctcc
    14221 gccgcgttcg cgcagatagg cgcgcaggcc gtcgcgccag tcgggcaggc ggatcgggtc
    14281 gggccactcg gtgtcgagca cggagtacgc cggccgcggc gccggccgcg cgaactcggc
    14341 ggtcgtcgtc gagagcacct ggcactcgac gccggcgtcc tcgaagatcg ctcgcgcgaa
    14401 gtcgtaccag gagcactgcc cgccggcggc catgtggtgg acgccgtagg cgggcgtcgc
    14461 ggcgagccgc agcagcccct cggcgaggtg ggcggtgtag gtcggcgagc cgacctggtc
    14521 gcgcacgacc gtcaccacgc cgccgcccgc gagcccgagc atcgtctcga cgaagttgcg
    14581 cccgccgacc ccgaacagcc acgcggtgcg gacgacgaag tggcgcgggt tggcggcggc
    14641 caccgcctgc tcgccggcga gcttcgtgcg gccgtaggcg gactgcgggc cggtcgggtc
    14701 ggactcgacg tacggccgcg tggacgtccc gtcgaagacg tagtcggtcg aaggctggac
    14761 gaccgacgcc cctacgctcg ccgccgccgc agcgacgttc cccgcggcgt ccgcgttcag
    14821 cctcagcgcc tcctcctcgg ccgtctcggc cccgtcgacg tccgtccagg ccgcgcagtt
    14881 gatcgcggcg tccggtcgca gctgcgcgaa cgcgtcgctc accgcacccg catccgtcac
    14941 gtccagcgcc gcccggtcga gcgcgtccac ctcgtggttg ccagcctccg ccgcccgccg
    15001 cacgtcctgg ccgagcatcc cgcccgcgcc ggtgatcaag accttcacgc cgcgagggat
    15061 atcagattcg gcattctgat caccatatat gctatgtcgg cgacctgaaa gacagcgtta
    15121 gctcatatcc aattggatat catttatctc atgagtccag gagttcaatt gcgactggcg
    15181 agaaatagag cgggccttac ccagaccgag ctagctcgcc gcgcgggtac gtcacaggcc
    15241 accatctcct cgtacgagag cggtcacaag cagccttcgg tcgacacgtt caaccggttg
    15301 ctggcggcaa ccggctcccg tctggtcgtt gagtcggggc gtcggcccat cctcttgccg
    15361 tcgcgtgcgc agcttgttcg gtcggcccga acgctgagcg atgttctgag cttggccgag
    15421 gcgcttcccg caagtcatga ccaggagctc cggttccccc ggctgccctc ccgcgcaagg
    15481 accgcgacgt gaagatccag aagcgcgtga tcgagctcca ccgggccttc gaccgacatg
    15541 agatcccgca tgcctttggc ggggcgctcg cgcttgcgta ctggacattg gacccaagag
    15601 ggacgaacga catcgacgtc aacctgttca tgccggcggc cgactgcgac cgagcactga
    15661 ggagcctgcc cgacggggtg gagcagccgg agggaaccgt cgatcggata cgccgcgacg
    15721 gccagatccg gctctggtgg gaggagatcc ctgtcgacct cttcttcgac tacgcgccag
    15781 tccacgagga tgcggcgcgg gaccgacagg tcgtcccgtt tctcgacatc gacatccccg
    15841 tgctcggccc agtcgagctt gctgtcttca aggcgatgtt cgatcgtacg aaggattggg
    15901 ccgacgtcga ggcgatgatc gcggcgcagc ggctcgacgt cgacctggta cgcgagtcgc
    15961 tgcgaaccat gctcgcgtcc gacgatcatc gcttcgccag gctcgacgag gccgagcggc
    16021 tggctgcgac tgcgtctccg ccgggctgac ggccacgggc tatggtgcgc ctggtgcgcc
    16081 gaggactaat cgccgtcgcg gttgccctgt tcgcgctgcc gagcggcgcc gcggcgcaga
    16141 ccgccgagcc gctggcagcg cagctcgagc cgccgttgcc gtcgccggtc gtcgcgggcg
    16201 attggcccga tcccgacgtg tcgctgatcg acggcagcta ctacgcggtg gcgacgagcg
    16261 gcggctggtc gccgacgttc cgggtgctgc agtcgacgga cctgcgctcg tggtcgatcg
    16321 ccgggtcggt cttcgggcgg ccgccgggct ggacgaagga ctcgttttgg gcgccggagc
    16381 tggcgccgct gccggcgggc ggctacgcgc tcttctacag cgcgatgccc aagcgcccgt
    16441 ggcagacgcg cgcggacgga agcacggtgc cctaccgtgg cgcgcgcgtg cggcccggcg
    16501 ggccgcaggt tccccgggct ggccggccct ggtactgcct cggcgtggcg accgctccct
    16561 cgccggtcgg cccatggcgc gacctcggcc gtccgctgcg ctgcacgcca cgcggcacga
    16621 tcgaccccac gcccgtcgtc gacggcgaca agctctacct cgtctacaag gaggacggca
    16681 acgccttccg caagccgacg ccgatcctgc tgcaggagct gcgcgccgac ggccggcggt
    16741 tgctcggcgc gccgcgtgag ctgctgcgca accagccgcg gacgtgggag cgcaacgtga
    16801 tcgaggcgcc gttcctggtt cgccgcgacg ggggctggac gatgctctac tcgggcgcac
    16861 tctgctgctc gccggattgc gcctacgcgg ttggcgcggc ccgcgccccg acgctcgcgg
    16921 ggccgtggac gcgctacccc ggcaatccga tcctgcgctc cggcaacggc tggcgctgcc
    16981 ccgggcacgt ctcggtcgtt ggcgacaacg
//
LOCUS       DATABV010000231        13358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1765980, whole genome shotgun sequence.
ACCESSION   DATABV010000231 DATABV010000000
VERSION     DATABV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13358
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765980"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VHF88_10705"
     CDS             complement(<1..405)
                     /locus_tag="VHF88_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX2162275.1"
                     /translation="MVDLIIYAVPAFVLLLVVEAVSFRVAQREDLKGYDLRDTRTSLS
                     MGIGNVIVNTGWKAIVLLIYVGLYELTPLRVPESAWWAWVALFFLDDFAYYWFHRISH
                     EVRVFWASHVVHHSSQYFNLSTALRQTWTPMTY"
     gene            448..1314
                     /locus_tag="VHF88_10710"
     CDS             448..1314
                     /locus_tag="VHF88_10710"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2162276.1"
                     /translation="MNDVVWTHRPSLRRPVLVAAFGGWNDAGDAASAAVEFIRSRFEP
                     TEIARIELEDYLDFTETRPTARLVDSRTREIDWPATTIAAAEVRGADNDLIVVQGPEP
                     GLRWRRYCETVITIAQELDVRRVVTLGSLLADVPHSRPVHVTGIGSDTALLDRLGFSP
                     TSYEGPTGITGVLNYEAGRMGLPSVSLWASVPHYVAAAPNPKVALALVRAFEGAAGLA
                     VDATDLEEAAEDYERQVNAAVASDPEVKAFVERLESAMDEVAEDDAIEPNIPSADMIA
                     SDFQRFLRQRRG"
     gene            complement(1410..4694)
                     /locus_tag="VHF88_10715"
     CDS             complement(1410..4694)
                     /locus_tag="VHF88_10715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX2162277.1"
                     /translation="MLAVLSAGLTEPQRAAASHPGGALAVTGVAGSGKTTVLAHRFAW
                     LAAPEREGAVRPERLLMLVHSSHAREAMCGLLGELVEPPYEELHVQTFDELCRALLAD
                     EALEAGLDPLFVPMTRADRVALLLDRLGDLTLRHHEIRGNPAPLLASFVERIDRLKEQ
                     MTTPEELLERTRAELAVAAEASDAARALAAREVEFAQLYADHDRLLADRGALDSGDLV
                     LTAFRLLHERPHVRERAARRFDHVLIDEYQEATFAQGMVLRLLCEEHGQVTVAGDDAQ
                     TLRRFGDVGGRNLADFPREYPDATVVDLGPSRRWPDPIAQVATAVAPPGDARMRDLHR
                     PGGTILHLAGVEGGPTGQMQGSHRPGGTSVHRAVGGGDVRFWRCASERAQAQAVALHV
                     ERLLAGGADPAAIAVMVRSAADDGTAVGGALEQRAIPFRVRGPGAFFQRAEVRDVLAW
                     LRVLADPADSGAAVRALTRPPIGLHSVDIARLTQLARRRKLDMPSAVAAALDGPQLSP
                     EGRDRAQAFLDLYRAAARAFESRRPDAFVLRLIERVGIRRQQVFATQADTVERLRNIA
                     RLPELATAYMRREPNATPRDFTRYLKAVADSGLPETEATGPEGAPAVAILGIDDAKGR
                     EFDHVFVLGLSAARMPGRPPALEPDALAVEAGPTTAPAVAPTHEQVMRRRLHIALSRA
                     RRSLVLSWSDAPNGSPARPSPFYEEARMAAGAPEERFEEELFGPAEGLHSTFRIMRDE
                     LLDTVQRIGGRLGEMRLDTTLDVSQAVARYLELLKVAALIERSKDGQPLDDAISEVNQ
                     LLAAGASAEQRAVLESSALDDWLRDTGRDPDRRPVAGDDHAGPSLDPFIPRRRGAGAP
                     EGGLMLSASDIETYRLCPLKYKFGRVFRIPQESTIHQRFGIALHTVLERFHKSPDAGR
                     ETLFELFESSWRRGGFGDSDDELQFRERGIEALERYWENTRDQEGEPVWFERSFSFQL
                     GPHVIRGRVDRVDRRPDGSYEVIDYKTGRSKTAAELQSDVQLSIYQMGARESWGIETS
                     AHSYYYVMTAEKVPVAHSDTELDRVRATIEEIGAGISRQDFQPTPSHEICSFCDFRII
                     CPAAER"
     gene            complement(4748..5383)
                     /locus_tag="VHF88_10720"
     CDS             complement(4748..5383)
                     /locus_tag="VHF88_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2162278.1"
                     /translation="MSSLTPHLDRSSARARTALLRPLLAALVLCALLLALAPPGADAA
                     RARISGYLPNCQVATAAASLAPRTAPTIRLARAAVCLINARRATRGLPRLRINHRLSR
                     AARWHTQDMVRRSYFGHVSRRGRDVVDRLYGARYLGGRFSWAVGENLAWGSGSLGTPR
                     SIVRAWMKSPGHRRNMLDRRYREIGIGVIAAGPVRTDIPAATYTTTFGVRH"
     gene            5454..6878
                     /gene="ligD"
                     /locus_tag="VHF88_10725"
     CDS             5454..6878
                     /gene="ligD"
                     /locus_tag="VHF88_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2162279.1"
                     /translation="MADRLRDYRDKRDFSVTPEPGDEGAPADADLPRFVVQEHHASHL
                     HWDLRLEREGTLASWAVPRGIPDHPEEDRLAVRTEDHPLKYLEFEGEIPAGNYGAGTM
                     AVWDSGTYELEKWRDDEVMAVFRGDRVQGRYVLFRTGGKNWMIHRMDPPAPGVEPMPE
                     AIEPMKARLGELPRDEDAFAYEVKWDGIRAVARIDRGHLDLTGRNGTDYRPRYPELRP
                     LGGVLGSTRAILDGEIVAFDEQGRPSFERLQSRMHLTGEAAVKRRSKDLPVTYVIFDL
                     LWLDGHLTTGLAYRDRRRLLSELELDGPRWRTPSHHEGDGGALLEATREQGLEGIMAK
                     RLDSTYEPGRRSGAWIKVKNVLRQEVVIGGWARGEGKRAGSFGALCVGVHDEEGKLRY
                     VGKVGTGFDESLLSLLTRELTDRRRDTSPFEGRQPPRGTVFVEPNLVGEVEFREWTRT
                     GTLRAPAFKGLRDDLDARRVVRES"
     gene            complement(6879..7307)
                     /locus_tag="VHF88_10730"
     CDS             complement(6879..7307)
                     /locus_tag="VHF88_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162280.1"
                     /translation="MSSLDLSRRPSRPAGLDRLVAVLLTSVAMSLAFGALSPGTGTGL
                     IDRVAPQSAEAFQGGWDRDHWWIKVTRAEVFQGVVVVACGRFVPWPYSMRVCGPIGQT
                     AKHAIRGGRGLWAEVYPPRWVWLRRGIGWWTPTRYRVGIW"
     gene            7432..8826
                     /locus_tag="VHF88_10735"
     CDS             7432..8826
                     /locus_tag="VHF88_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162281.1"
                     /translation="MSKADALIDAAIARLARGDGEGSLRDLGIDRPQLVRDSGVSRGV
                     IDYHFAQRGQGDALRGLADAVLERLLAEQLRLREEASGRYNQRAQFVADGAGRRTIAQ
                     ALLDELARYGAVADADDDLTRARERAYYLAVALCDVRSSDSQRPIAEDGNHVPDHRFR
                     RHVVEMHQRPRAALLQVYADFLATAGREPEPDIERLVLVMSAFLEGVVLRRRIAHGHG
                     EETAHARAAERPLALGDDDLVDAALRIFIAMSRPVGGSRPDPDAILFARDGAAAQAAS
                     ETVLHRDRAALYETILEAIGQLSAEDALSHCALHTSGVGRSRSEQGEALKRSLADFLD
                     RGGRLRNIEKVNSVAELDATVTRLAAGRDAGHDQTFRVLVMDSPPGLSPLLIGDRMAL
                     LGRDVDGQIVDGVAFCDAAGREWCETHYDSLWHDDRAYTLASPNGLNDRGIGDARRQL
                     EELERRRKFPATAG"
     gene            8857..9792
                     /locus_tag="VHF88_10740"
     CDS             8857..9792
                     /locus_tag="VHF88_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX2162282.1"
                     /translation="MPRAIWSGAISFGLVNIPVKLYSAVSRKTVRFNQLDGKDGTRIQ
                     QKRVNPKTGDEVPYERIVKGYEISPDRYVVVKPEELESVEPRKTHMIEIEDFVEIDQI
                     DPIFYDHPYYLAPGKGATKAYALLLKAMEESGRVGIARVVIRSKEQLVALRAQDGVLH
                     METMLFGDEVVSPDSLDEIPDADELEAKPREVKMAGELIESLASDFDPSKYRDEYREQ
                     VLDLIERKAEGEEIAVQPQEEERPEVPDLMAALEASIASAKRQSGTAAEKKPAAADDG
                     KAKAKKPAAKKKPAAKAKAPAKKRAPAKKKPAAKK"
     gene            9793..10674
                     /gene="ligD"
                     /locus_tag="VHF88_10745"
     CDS             9793..10674
                     /gene="ligD"
                     /locus_tag="VHF88_10745"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2162283.1"
                     /translation="MAPEVDVEGRRLKLSNLDKVLYPATGFTKGQVIDYYTRVAPTLL
                     AHLRGRALTLKRYPNGVEGKHFYEKQRPSHAPDWVRSKTIPTGDRDIDFVLCDDLATL
                     VWLANLADIEMHPSLALAKRPGRPTVVAFDLDPGEPAALPECREVALLLRDALAGVGL
                     ECCAKTSGSKGIQVYVPLNTPVDYENGTKPFALAVAQLLEKHHPKLVVSSMKKSLRGG
                     KVLIDWSQNSFHKTTVAAYSLRAKERPTVSTPVSWDEIEDDSLDLVFETGDVLERIEQ
                     HGDLFVPVLEKKQKLPG"
     gene            10740..11618
                     /locus_tag="VHF88_10750"
     CDS             10740..11618
                     /locus_tag="VHF88_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162284.1"
                     /translation="MDVARMAIAALAVFVGSNAVWACADGDSSGEPTRVVIEVRDPSP
                     ARVALSAPRSVPAGTVTIELRNRGDTHHDAQLFRVDNGRTSAEVVGVLESSDSASKPG
                     WLHPAGGVAATSPGDTATVTQILAPGTYYVADTQERAMANEARRTNATKGGIVRFVVE
                     GEADAELPKTAATITASDDGYKTDGIVAGPNRVTFRNAGKELHQAVALPIDEELGYRE
                     GRRRALARHDDTGWVPVDVRHERATAVLEDGGEQIVELTFDPGRYVLLCFVSPRAGGA
                     AQWQLGMSSELEARRR"
     gene            11661..12857
                     /locus_tag="VHF88_10755"
     CDS             11661..12857
                     /locus_tag="VHF88_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2162285.1"
                     /translation="MVDWTIARRVAGFATRSEDRVIDPGLDLLALAAEFEPRVSEYTR
                     LTPVAPIPSPELVSRHGWADANLDAMAELMKPVEGRMKERFEKAGPLAGPLRAGAGAA
                     LATELGLVLGYMSQRVLGQYELSLIAGTANPRLLFVGANLVGAAKTLGVDREAFVRWV
                     TVHELTHAMQFGGVPWLRDHMGGLLKEYLETVDVRLDGGAANDAAGADDQDPASPASP
                     DAGHAPQTPPSSGARRRSGGLLGSALEKLRALDVPDPTELAERFRDGGLAALVQNDEQ
                     RDVMERMQAAMAVVEGHAEHVMDALAPDLVPQHEPLRAAMDARRQSRSAPERILMRLL
                     GMEMKLRQYKLGKEFCDAVVAESGIEGLNRVWAGPEALPTLAEIERPADWLARQAAGE
                     NRLAAG"
     gene            12998..>13358
                     /locus_tag="VHF88_10760"
     CDS             12998..>13358
                     /locus_tag="VHF88_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2162286.1"
                     /translation="MTTTPWGDATQLRARRLRPGPGANPAAVTRNQRERMYAAMVAAV
                     AENGYEGTRVADVVKLSGVSRSAFYKHFEDKLDCFLATLDELAEMAGKELAKHYDESL
                     PGEERLRTVADAFLDLVL"
BASE COUNT         2022 a         4625 c         4975 g         1736 t
ORIGIN      
        1 gtaggtcatc ggcgtccagg tctggcgcag cgcggtcgag aggttgaagt actgcgagct
       61 gtggtggacg acgtggctcg cccagaagac gcgcacctcg tggctgatgc ggtggaacca
      121 gtagtaggcg aagtcgtcga ggaagaagag cgcgacccag gcccaccacg cgctctccgg
      181 aacgcgcagc ggcgtcagct cgtagaggcc gacgtagatc agcagcacga tcgccttcca
      241 gcccgtgttg acgatcacgt tgccgatccc catgctgagg ctggtgcgcg tgtcgcgcag
      301 gtcatagccc ttgaggtcct cgcgctgggc gacgcggaac gagaccgcct cgacgaccag
      361 cagcaggacg aacgccggga ccgcgtagat gatcaggtcg accactgtcg gtagaggcta
      421 ccgccgcgcg ataccgtggg tggggacttg aacgacgtcg tctggaccca ccggccgtct
      481 ctgcgccgcc ccgtgctcgt ggcggcgttc ggaggatgga acgacgccgg cgacgcggcg
      541 tcggccgcag tggagttcat ccgctcgcgc ttcgagccga ccgagatagc gcggatcgag
      601 ctcgaggact acctcgactt caccgagacg cggccgacgg cgcggctggt cgacagccgt
      661 acgcgcgaga tcgactggcc ggcgacgacg atcgcggcgg ccgaggttcg cggcgcggac
      721 aacgacctga tcgtcgtcca ggggccggag ccggggctgc gctggcggcg ctactgcgag
      781 acggtgatca cgatcgccca ggagctcgac gtgcggcgcg tggtgacgct cggctcgctg
      841 ctcgccgacg tgccgcactc gcgcccggtg cacgtgacgg ggatcggctc cgacacggcg
      901 ctgctcgacc ggctcgggtt ctcgccgacc agctacgagg gcccgaccgg gatcaccggc
      961 gtgctcaact acgaggccgg ccggatgggc ctgccgagcg tcagcctctg ggcgtcggtg
     1021 ccgcactatg ttgcggccgc gccgaaccct aaggtggcgc tggcgctggt gcgggcgttc
     1081 gagggtgccg cgggcctggc ggtggacgcg accgacctcg aggaggccgc cgaggactac
     1141 gagcgccagg tcaacgccgc ggtcgccagc gacccggagg tcaaggcgtt cgtcgagcgg
     1201 ttggagtccg cgatggacga ggtcgcggag gacgacgcga tcgagccgaa catcccgtcc
     1261 gccgacatga tcgcctcgga cttccagcgg ttcctgcgcc agcggcgcgg ctagccgcgc
     1321 ccggcggccc gcggtaacgc gctcgatgtg ggattggcgc gtcctggcga tgcaaattcg
     1381 acatcgagcg gctacccggc ggggcgggct taccgctcgg cggccgggca gatgatgcgg
     1441 aagtcgcaga aggagcagat ctcgtggctt ggggtcggct ggaagtcctg gcgcgagatg
     1501 ccggcgccga tctcctcgat cgtggcgcgg acgcggtcga gctcggtgtc ggagtgggcc
     1561 acggggacct tctctgcggt catcacgtag tagtaggagt gggccgaggt ctcgatgccc
     1621 caggactcgc gcgcgcccat ctggtagatc gagagctgga cgtcgctctg cagctcggcc
     1681 gcggttttgg agcggccggt cttgtagtcg atgacctcgt agctgccgtc gggacggcgg
     1741 tcgacgcggt cgacgcggcc gcggatcacg tgcgggccga gctggaagga gaagctgcgc
     1801 tcgaaccaga ccggctcgcc ctcctggtcg cgtgtgttct cccagtagcg ctcgagcgcc
     1861 tcgatgccgc gctcacggaa ctgcagctcg tcgtcggagt cgccgaagcc gccgcgccgc
     1921 caggaggact cgaagagctc gaagagcgtc tcgcggccgg cgtcgggcga cttgtggaag
     1981 cgctcgagga cggtgtgcag ggcgatcccg aagcgctggt ggatcgtcga ctcctgcggg
     2041 atccggaaga cgcggccgaa cttgtacttg agcgggcaga ggcggtaggt ctcgatgtct
     2101 gaggccgaga gcatcagacc gccctcgggc gccccggcgc cgcggcggcg cgggatgaac
     2161 gggtcgagcg acgggccggc gtggtcgtcg ccggcgacgg ggcggcggtc ggggtcgcgg
     2221 ccggtgtcgc gcagccagtc gtcgagcgcg gaggactcga gcacggcgcg ctgctcggct
     2281 gacgcgccgg cggcgagcag ctggttgacc tcggagatcg cgtcgtcgag cggctgcccg
     2341 tccttggagc gctcgatcag cgcggcgacc ttgagcagct cgaggtagcg ggcgaccgcc
     2401 tgcgagacgt cgagcgtcgt gtccaggcgc atctcgccga gccggccccc gatccgctgg
     2461 acggtgtcga gcagctcgtc gcgcatgatc cggaaggtgg agtgcaggcc ctcggccggg
     2521 ccgaagagct cctcctcgaa gcgctcctcg ggggcgccgg cggccatgcg agcctcttcg
     2581 tagaagggcg aggggcgggc cggcgagccg ttcggggcgt cggaccagga gaggacgagg
     2641 ctccggcgtg cgcggctcag cgcgatatgc aggcggcgcc gcatgacctg ctcatgggtc
     2701 ggcgcgacgg ccggcgcggt cgtcgggccg gcctcaacgg cgagcgcgtc cggctccaaa
     2761 gcgggcgggc gtccgggcat gcgcgcggcc gacaggccga gcacgaagac gtggtcgaac
     2821 tcgcggccct tggcgtcgtc gatgcctagg atcgcgacgg cgggagcgcc ctccggtccg
     2881 gtggcctcgg tctctggcag gccggagtcg gcgacggcct tgaggtagcg ggtgaagtcg
     2941 cgcggcgtgg cgttcggctc gcggcgcatg taggcggtgg cgagctcggg caggcgggcg
     3001 atgttgcgca gccgctcgac ggtgtcggcc tgggtggcga agacctgctg ccggcggatg
     3061 ccgacgcgct cgatcaggcg cagcacgaag gcgtcggggc ggcggctctc gaaggcgcgc
     3121 gcggcggcgc ggtagaggtc gaggaaggcc tgggcgcggt cgcggccctc gggggagagc
     3181 tgcggcccgt cgagcgcggc ggcgacggcg gacggcatgt cgagcttgcg ccggcgggcg
     3241 agctgggtga gccgcgcgat gtcgaccgag tgcaggccaa tcggcgggcg ggtcagcgcg
     3301 cgcaccgcgg cgcccgagtc ggccgggtcg gcgagcacgc gcagccaggc gagcacgtcg
     3361 cgcacctcgg cacgctggaa gaaggcgccc gggccgcgga cgcggaaggg gatcgcgcgc
     3421 tgctccaggg cgccgccgac ggccgtgccg tcgtcggccg ccgagcggac catcacggcg
     3481 atcgctgccg ggtcggcgcc gccggcgagg agtcgctcga cgtggagcgc caccgcctgc
     3541 gcctgagcgc gctcggacgc gcagcgccag aagcggacgt cgccgccccc gaccgcccga
     3601 tgcacgcttg tgccccctgg gcgatgcgaa ccctgcatct gaccggtggg gccgccctcc
     3661 acgcccgcta gatgcaggat tgtgccccct gggcgatgca aatcccgcat ccgagcgtcg
     3721 cctggaggcg cgacggcggt ggcgacttgc gcgatcgggt ccggccagcg ccggctcggg
     3781 ccgaggtcga cgacggtcgc atccggatac tcgcgcggga agtcggcgag gttgcggcca
     3841 ccgacgtccc cgaagcggcg cagcgtctgg gcgtcgtcgc cggcgacggt gacctggccg
     3901 tgctcctcgc agagcaggcg cagcaccatg ccctgggcga aggtcgcctc ctggtactcg
     3961 tcgatgagca catggtcgaa gcgacgcgcc gctcgctcgc ggacgtgggg gcgctcgtgc
     4021 agcaggcgga aggcggtcag gaccaggtcg ccgctgtcga gcgcgccgcg gtcggcgagc
     4081 agccggtcgt ggtccgcgta gagctgggcg aactcgacct cgcgggcggc gagcgcgcgt
     4141 gccgcatcgc tggcctcggc cgccacggct agctcggccc gcgtccgctc gagcagctcc
     4201 tcgggcgtcg tcatctgctc cttgagccga tcgatccgct cgacgaagct cgcgagcagc
     4261 ggcgccgggt tgccgcggat ctcgtggtgg cgcagcgtca ggtcgccgag ccggtcgagc
     4321 agcagcgcca cgcggtcggc gcgggtcatc gggacgaaca gcgggtcgag cccggcctcg
     4381 agcgcctcgt cggcgagcag cgcccggcag agctcgtcga aggtctggac gtgcaactcc
     4441 tcgtagggcg gctcgacgag ctcccccagc agcccgcaca tcgcctcgcg cgcgtgcgag
     4501 gagtggacca gcatcagcag ccgctcgggc cggacggccc cctcgcgctc gggcgccgcc
     4561 agccatgcga agcggtgcgc cagcacggtc gtcttccccg agcccgcaac tcccgtcacc
     4621 gctagcgcgc ccccggggtg gctcgccgcc gcccgctgcg gctcagtcag gccagctgaa
     4681 aggacggcga gcatgccggc caaggatacg gcggcacgaa cggcttccca tagcgcccgt
     4741 acaggcgcta gtgccggacg ccgaaagtcg tcgtgtaggt cgcggcgggg atgtcggtgc
     4801 ggacggggcc ggcggcgatg acgccgatgc cgatctcgcg gtagcggcgg tcgagcatgt
     4861 tgcggcggtg gccggggctc ttcatccagg cgcggacgat cgagcggggg gtgccgagcg
     4921 agccggagcc ccaggcgagg ttctcgccga cggcccagct gaagcggccg ccgaggtagc
     4981 gggcgccgta gaggcggtcg acgacgtcgc ggccgcgccg ggagacgtgg ccgaagtagc
     5041 tgcggcggac catgtcctgg gtgtgccagc gggcggcgcg gctgagccga tggttgatcc
     5101 gcaggcgcgg caggccgcgg gttgcgcgtc tggcgttgat caggcagacg gcggcgcggg
     5161 cgaggcggat cgtcggcgcg gtgcgcggcg ccaaggaggc ggccgcggtc gcgacctggc
     5221 agttcggcag gtagccgctg atccgcgcgc gggcggcgtc ggcgccgggc ggcgcgaggg
     5281 ccagcagcag cgcgcagagg acgagggcgg cgagcagcgg gcgcagcagc gccgtgcggg
     5341 cccgtgcgga ggaccgatcg agatgggggg tcagcgagct catcgcccac tgagtcgtcc
     5401 gctcctctcc gcacctttag ccccggcgag attcgccccg ccacaatgga cgggtggccg
     5461 accgtctgcg cgattaccgc gacaagcgtg acttctcggt cacgcccgag ccgggcgacg
     5521 agggagcgcc cgccgacgcc gatctgccgc gcttcgtcgt ccaggagcac cacgcgagcc
     5581 acctgcactg ggacctgagg ctcgagcgcg aggggacgct ggcgtcgtgg gcggtgccgc
     5641 gcgggatacc ggaccacccc gaggaggacc ggctcgccgt gcggaccgag gaccacccgc
     5701 tgaagtacct cgagttcgag ggcgagatcc ccgccggcaa ctacggcgcc ggcacgatgg
     5761 ccgtctggga cagcggcacc tacgagctcg agaagtggcg cgacgacgag gtgatggcgg
     5821 tcttccgcgg cgaccgcgtc cagggccgct acgtcctctt ccgcaccggc ggcaagaact
     5881 ggatgatcca ccggatggac ccgccggcgc cgggggtcga gccgatgccg gaggcgatcg
     5941 agccgatgaa ggcgcgactc ggcgagctgc cgcgcgacga ggacgcgttc gcctacgagg
     6001 tcaagtggga cggcattcgc gcggtggcgc ggatcgaccg cggccacctc gacctgaccg
     6061 gtcgcaacgg gaccgactac cgcccgcgct atcccgagct gcgtccgctc ggcggcgtgc
     6121 tcgggtcgac gcgcgcgatc ctcgacggcg agatcgtcgc cttcgacgag cagggccggc
     6181 cgagcttcga gcggctccag tcgcgcatgc acctgaccgg cgaggcggcg gtcaagcggc
     6241 gctccaagga cctgccggtg acctacgtga tcttcgacct gctctggctc gacggccacc
     6301 tgacgaccgg gctcgcctac cgcgaccgcc gccgcctgct ctccgagctc gagctcgacg
     6361 gcccgcgctg gcgcacgccg agccaccacg agggcgacgg cggcgcgctg ctcgaggcca
     6421 cccgcgagca gggcctcgag ggcatcatgg ccaagcggct cgactcgacg tatgagcccg
     6481 gccgccgctc cggcgcctgg atcaaggtca agaacgtcct gcgccaggag gtcgtgatcg
     6541 gcggctgggc gcgcggcgag ggcaagcgcg ccggcagctt cggcgccctc tgcgtcggcg
     6601 tccacgacga ggagggcaag ctgcgctacg tcggcaaggt cggcaccggc ttcgacgaga
     6661 gcctgctctc gctgctgacg cgcgagctga ccgaccgccg ccgcgacacc agccccttcg
     6721 aaggccgcca gcccccgcgc ggcacggtct tcgtcgagcc caacctggtc ggcgaggtcg
     6781 agttccgcga gtggacacgg accggcaccc tgcgcgcccc cgccttcaag ggcctgcgcg
     6841 acgacctgga cgcgcgcagg gtggtccgtg agagttgact accagatgcc gacccggtac
     6901 cgcgtcggcg tccaccagcc gatacctcgc ctgagccaga cccagcgcgg cgggtagacc
     6961 tcggcccaga gcccgcgccc gccacggatc gcgtgcttgg cggtctggcc gatcgggccg
     7021 cagacgcgca tgctgtaggg ccagggcacg aagcgcccgc aggcgaccac cacgacccct
     7081 tggaacacct ccgcgcgagt caccttgatc caccagtggt cgcgatccca tccgccctgg
     7141 aacgcctccg cggactgcgg cgcgacccgg tcgatcaggc cggtgccggt ccccggcgac
     7201 agagcgccga acgccaggga catcgccacc gaggtcaaca ggaccgcgac gagccggtcg
     7261 aggccggcgg gcctcgaggg tcgtcgcgac aagtcgagtg agctcatgcc acacctttcg
     7321 atcgttgcct tgccgcgact gtacgagagt cttcctgaca cttcggccca gcgcagaggc
     7381 actttcgtca acgttttggc gaatttgtca attcgctgtt tagatggcgc gatgtcgaag
     7441 gcagacgcac tcatcgacgc ggcgatcgcc cgcctcgcgc gcggcgacgg cgagggctcg
     7501 ctgcgcgacc tcggaatcga tcgcccgcag ctcgtccgcg actcgggggt cagccggggc
     7561 gtgatcgact accacttcgc ccagcgcggc cagggagacg ccttgcgcgg gctggccgac
     7621 gcggtgctgg agcgcctgct cgccgagcag ctgcggctgc gcgaggaggc ctcggggcgc
     7681 tacaaccagc gagcccagtt cgtcgcggac ggcgccggcc gccggacgat cgcgcaagcc
     7741 ctgctcgacg agctcgcccg ctacggggcg gtcgcagacg ccgacgacga cctgacacgc
     7801 gcgcgcgagc gcgcctacta cctggcggtg gcactctgcg acgtcaggag cagcgactct
     7861 cagcgtccga tcgccgagga cggcaaccac gtacccgacc accgcttccg gcgccacgtc
     7921 gtcgagatgc accagcgccc ccgagccgcc ctgctccagg tctacgcgga cttcctggcg
     7981 acggccggcc gcgagccgga gccggacatc gagcgcctcg tgctcgtcat gagcgcgttc
     8041 ctggagggcg tcgtcctgcg gcgccggatc gctcacggcc acggcgagga gaccgcccac
     8101 gcgcgggcgg ccgagcggcc gttggcgctc ggtgacgacg acctcgtcga cgccgcgctg
     8161 cggatcttca tcgcgatgtc gcgaccggtc ggcggctcgc ggcccgaccc cgacgccatc
     8221 ctcttcgccc gcgacggcgc cgctgcccaa gcggcgtcgg agacggtcct ccaccgcgac
     8281 cgcgcggcgc tctacgagac gatcctcgag gcgatcggcc agctctccgc cgaggacgcg
     8341 ctttcgcact gcgcgctgca cacctcgggc gtcgggcgct cacgcagcga gcagggcgag
     8401 gccctcaagc gcagcctcgc cgacttcctc gaccgcggcg gccggctgcg caacatcgag
     8461 aaggtcaact cggtcgccga gctcgacgcg accgtgacgc gactggcggc gggaagggac
     8521 gccggccacg accagacgtt ccgcgtcctg gtcatggact cgccgcccgg gctgtcgcca
     8581 ctgttgatcg gcgatcgcat ggcgctcctc ggacgcgacg tcgacggcca gatcgtggat
     8641 ggcgtggcct tctgcgacgc ggccgggcgg gagtggtgcg agacgcacta cgacagcctc
     8701 tggcacgacg accgcgccta cacgctcgcg agcccgaacg gcctcaacga tcgcggcatc
     8761 ggcgacgcgc gccgccagct ggaggagctc gagcggcggc gcaagtttcc agccaccgcc
     8821 ggatagggac tcgacaggcg gggtaccgtt cgaggcatgc ccagggcaat ctggagcggg
     8881 gcgatcagct tcggcctcgt gaacatcccg gtgaagctgt actcggcggt ctcgcgcaag
     8941 accgtgcgct tcaaccagct cgacggcaag gacgggacgc ggatccagca gaagcgtgtc
     9001 aacccgaaga cgggcgacga ggtcccgtac gagcggatcg tcaagggcta tgagatctcg
     9061 ccggaccgct acgtggtggt caagccggag gagctcgagt cggtcgagcc gcgcaagacg
     9121 cacatgatcg agatcgagga cttcgtcgag atcgaccaga tcgacccgat cttctacgac
     9181 cacccctact acctcgcgcc cggcaagggc gcgaccaagg cgtacgcgct gctgctcaag
     9241 gcgatggagg agtcgggccg cgtcgggatc gcgcgcgtgg tgatccgctc gaaggagcag
     9301 ctcgtcgcgc tgcgcgccca ggacggcgtg ctgcacatgg agacgatgct gttcggcgat
     9361 gaggtggtct cgcccgactc gctcgacgag atcccggacg ccgacgagct cgaggccaag
     9421 ccgcgcgagg tgaagatggc cggcgagctg atcgagtcgc tggcgagcga cttcgacccg
     9481 tcgaaatacc gcgacgagta ccgcgagcag gtgctcgacc tgatcgagcg caaggcggag
     9541 ggcgaggaga tcgcggtcca gccgcaggag gaggagcggc cggaggttcc cgacctgatg
     9601 gccgcgctcg aggccagcat cgcgagcgcc aagcgccaga gcgggacggc ggcggagaag
     9661 aagcccgcgg cggccgatga cggcaaggcc aaggccaaga agcccgcggc caagaagaag
     9721 cccgccgcca aggccaaggc gcccgccaag aagcgcgcgc cggccaagaa gaagccggca
     9781 gccaagaagt aggtggcgcc cgaggtcgac gtcgagggcc ggcggcttaa gctgtcgaac
     9841 ctcgacaagg tcctctatcc ggcgaccggc ttcaccaagg gccaggtcat cgactactac
     9901 acgcgcgtcg cgccgacgct gctggcccac ttgcgcggcc gcgcgctgac tctcaagcgc
     9961 tatccgaacg gcgtcgaggg aaagcacttc tacgagaagc agcggccgtc ccacgcgccc
    10021 gactgggtcc gatcgaagac gatcccgacc ggcgaccgcg acatcgactt cgtcctctgc
    10081 gacgacctcg cgacgctcgt ctggctcgcc aacctcgccg acatcgagat gcatccctcg
    10141 ctcgcgctcg ccaagcggcc cggccgcccc acggtggtcg ccttcgacct cgaccccggc
    10201 gagcccgccg cgctgcccga gtgccgcgag gtcgcgctgc tgctgcgcga cgcgctcgcc
    10261 ggcgtcggcc tcgagtgctg cgcgaagacg tcgggctcga agggcatcca ggtctacgtc
    10321 ccgctcaaca cgccggtcga ctacgagaac ggcaccaagc cgttcgccct cgcggtcgcc
    10381 cagctactcg agaagcacca cccgaagctg gtcgtctcct cgatgaagaa gagcctgcgc
    10441 ggcggcaagg tcctgatcga ctggagccag aactcgttcc acaagacgac cgtcgccgcc
    10501 tactcgctgc gcgcgaagga gcgtccgacc gtctcgacgc ccgtcagctg ggacgagatc
    10561 gaggacgaca gcctcgacct cgtcttcgag accggcgacg tcctcgagcg gatcgagcag
    10621 cacggcgacc tgttcgtgcc cgtgctcgag aagaagcaaa agctgccggg ctagaccggc
    10681 gcctgtaggc tggcgccgtc agcgctcgag cgctgacgct gttcgacggg agggagtcga
    10741 tggacgttgc gcgaatggcc atagcggcgc tcgccgtgtt cgtggggtcg aatgccgtct
    10801 gggcctgcgc cgacggcgac tcgtcggggg agcccacgcg ggtcgtgatc gaggtccggg
    10861 accccagccc cgcgcgggtc gcgctcagcg cgccgcggtc ggtccccgcc ggcacggtga
    10921 cgatcgagct gcgcaaccgc ggcgacaccc accatgacgc gcagctgttc cgcgtcgaca
    10981 acgggcgcac gtccgccgag gtggtgggcg tcctcgagag ctccgactcg gcatccaagc
    11041 ccggctggct gcacccggcg ggcggcgtcg cggcgacatc tcccggcgac acggcgacgg
    11101 tgacccagat cctcgcgccg gggacgtact acgtggccga cacgcaggag cgcgcgatgg
    11161 cgaacgaggc tcgccggacg aacgccacga agggcggcat cgtccgcttc gtagtcgagg
    11221 gcgaggccga tgccgagctg ccgaagaccg ccgccacgat caccgcgagc gacgacggct
    11281 acaagaccga cggcatcgtc gccggcccca accgcgtgac cttccgcaac gccggcaaag
    11341 agcttcacca ggcagtcgcc ctgccgatcg acgaggaact cggctatcgc gagggcaggc
    11401 gcagggcgct cgctcgccac gacgacaccg gctgggtccc ggtcgacgtg cgccacgagc
    11461 gcgccacggc cgtgctcgag gacggcggcg agcagatcgt cgagctgacg ttcgacccag
    11521 ggcgctacgt gctgctctgc ttcgtctcgc cccgtgcggg tggcgcggcg cagtggcagc
    11581 tcggcatgtc gtccgagctc gaggcgcgcc ggcgctgagc gcgtcgggcc ggcggacgag
    11641 cgcaagctaa ggtcaaccga atggtcgact ggacgattgc gcgcagggtc gccgggttcg
    11701 cgacgaggtc ggaggaccgc gtgatcgatc ccggcctcga tctgctcgcg ctggcggcgg
    11761 agttcgagcc gcgggtcagc gagtacacgc gcttgacgcc ggtcgcgccg atcccgtctc
    11821 cggagctggt cagccgtcac ggctgggccg acgcgaacct cgacgcgatg gccgagctga
    11881 tgaagcccgt cgagggacgg atgaaagagc gcttcgagaa ggcgggaccg ctggcggggc
    11941 cgctgcgtgc gggcgccggc gccgcgctgg ccaccgagct cgggctcgtg ctcggctaca
    12001 tgtcgcagcg cgtgctcggt cagtacgagc tgtcgctgat cgccgggacc gccaacccgc
    12061 ggctgctgtt cgttggcgcg aacctcgtcg gagcggcgaa gacgctcggc gtcgaccgcg
    12121 aggcgttcgt ccgctgggtg accgtccacg agctgaccca cgcgatgcag ttcggcggcg
    12181 tgccgtggct gcgcgaccac atgggcggac tgctgaagga gtacctcgag accgtcgacg
    12241 tcaggctcga cggaggggct gcgaacgacg cagccggtgc cgacgaccag gatccggcca
    12301 gccctgcgtc ccccgacgcc ggccacgcac cgcaaacgcc accctcgagc ggcgcccgta
    12361 ggcgtagcgg cggcctgctc ggcagcgcgc tcgagaagct gcgcgcgctc gacgtccccg
    12421 atcccacgga gctggccgag cgcttccggg acggcggcct cgcggcgctc gtccagaacg
    12481 acgagcagcg cgacgtgatg gagcggatgc aggcggcgat ggcggtcgtc gagggccacg
    12541 ccgagcacgt tatggacgcg ctcgcgccgg acctcgtccc ccagcacgag ccgttgcgag
    12601 cggcgatgga cgcccgccgg cagagccgct cggccccgga gcggatcctg atgcgcctac
    12661 tcgggatgga gatgaagctg cgtcagtaca agctcggcaa ggagttctgc gacgcggtcg
    12721 tcgcggagtc ggggatcgag gggctgaacc gggtctgggc cggcccggag gcgcttccga
    12781 cgctggccga gatcgagcgg cccgcggact ggctcgcccg gcaggcggcg ggcgagaacc
    12841 ggctcgcagc gggctaagcg acacgcccgc ataggcctcg cctgccgtgc ggcagagccg
    12901 cttcggccct attccaaagt gctgaatttg ggaacgcggt cttacccgcg atcccgctcc
    12961 tgaagcgggc cggcgagacc actagagtcc tcgcgctatg accaccacgc catggggtga
    13021 tgcgacccag ctgcgtgcgc gtcgactgcg gcccggcccc ggggcgaacc cggcagcggt
    13081 cacccgcaat cagcgcgagc ggatgtacgc cgcgatggtc gcggcggtcg ccgagaacgg
    13141 ctacgaggga acgcgtgtcg ccgacgtggt caagctctcc ggcgtctcgc gcagcgcctt
    13201 ctacaagcac ttcgaggaca agctcgactg cttcctggcg acgctcgacg agctcgccga
    13261 gatggcgggc aaggagctcg cgaagcacta cgacgagtcg ctgcccgggg aggagcggct
    13321 gcggaccgtc gccgacgcct tcctcgacct cgtgctcg
//
LOCUS       DATABV010000232        18302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U6707
            k141_1124353, whole genome shotgun sequence.
ACCESSION   DATABV010000232 DATABV010000000
VERSION     DATABV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955329
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:00:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,119
            CDSs (with protein)               :: 2,119
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18302
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124353"
                     /isolate="SMAG_U6707"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..860)
                     /locus_tag="VHF88_10765"
     CDS             complement(75..860)
                     /locus_tag="VHF88_10765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2162287.1"
                     /translation="MSETAAARRAIVTGSDSGIGRAAAVALAEAGYDVGITWHEDREG
                     AAGTAAEVERHGRRAVVEQLDLADAVAAAATIRTLVDALGGLDLLVNNAGGAASHPFL
                     EYPLADWQAAIDVNLTGTFACAQAGARVLVAGDGGAIVNVTSVHEHIPLRDSAAYCAA
                     KGGLGLLTKVMAIELAEHDITVNAVAPGEIATPMTGNEDVPPDSVERPMIPAGRPGDA
                     REIAAAIVFLASPGGRYVTGESLVIDGGLSIMGAEANRIAGDG"
     gene            complement(869..1678)
                     /locus_tag="VHF88_10770"
     CDS             complement(869..1678)
                     /locus_tag="VHF88_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2162288.1"
                     /translation="MSVSSTARQLAVVTGASSGIGRELAAQFAAHDFDLVIAAEDSDI
                     ETVAMGIRDAGTSVDAVQVDLATDGGVDELYARIEAAGRPVDALALNAGVGEGGAFAT
                     DTDLAAELRLIDLNVRSTVHLAKHVLPGMVARGQGRILFTSSIASTMPGSFQAVYNAS
                     KSFVQSFALALREEVKDSGVTVTTLMPGPTDTEFFARAGMLDTRVGSSSHKDDPADVA
                     RDGFAAMMKGAERVVSASATTKLQGRSSRLLPDRVKAMMHRRMAEPRSGKR"
     gene            1766..2314
                     /locus_tag="VHF88_10775"
     CDS             1766..2314
                     /locus_tag="VHF88_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162289.1"
                     /translation="MRVLAGCLLAVAAVGCAADDDRSATAAPATAAMTDVPCEPYGVG
                     TGDVTPRDISIGPLTLIGGRNSASRRRNAFGGHGYKIPATLLAGKQATLSVPPRWRGK
                     VGLIYTQADQRRAWERGVRGAPRAVRFTACASSEPGARSGWPGGIVVDRPRCATLVVR
                     VAGELDEFRARVPLGRRCPRRS"
     gene            complement(2316..4133)
                     /locus_tag="VHF88_10780"
     CDS             complement(2316..4133)
                     /locus_tag="VHF88_10780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX2162290.1"
                     /translation="MTNEAIAEPRDEALEAARWDLQPLVHGRGAEGVLALMDEAQEAA
                     DAFAERYRGKVAELDAAGLAAALHELEGLSDKVGRAGSYAALDFSTDTQDPARGALMQ
                     QAQERGAAIQTALLFFDLEWNELPDEQAEELIASDELAFCRHYLRTLRRYRPHQLSEP
                     EERVLTEVDVTGRAAFKRLFTEQVAALTVDLPDSDEPVQLMEALSLLQGPDRELRRAA
                     AEGVTASLQPGLRTRAFILNTLLADKATKDRLRSYEHWIASRNLANEASDESVQALID
                     AVQSRYELARRWYRLKAKLLGVDKLAYYDRAAPVAESEQRVSYEDARQLVLDCYRSFS
                     PELGAVAEEFFTGSYIDAPPAPGKRGGAFCSYTVPSAHPYVLLNHTGRIGDALTMAHE
                     LGHGVHAALARPRGVFEFTTPLTVAETASIVGETIVLERLLTQAPDAAARLSLLAESL
                     DGAVAAVFRQVAMNRFEDRVHNERRTAGELSVDSINAAWLDTQADLLGDSVEPSEDYG
                     TWWSYVPHFIDTPGYVYAYAYGNLLALSVYRRYEEQGDSFVPSYLELLRAGGSMPPEE
                     LGAIVGVDLTDSGFWSAGLDLIERQLEAAETAAAEAGKI"
     gene            complement(4150..4524)
                     /locus_tag="VHF88_10785"
     CDS             complement(4150..4524)
                     /locus_tag="VHF88_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162291.1"
                     /translation="MSTSVKMSALLAVGRIATGAALLASPGRLGALWLGGAAANPGTQ
                     LAIRALGVRDLVLGVGALSALGDERRLRRWIGAGAVCDLTDAAGAMATPATALPDGAR
                     EGTVAMGAGAALAGALTHRALR"
     gene            4631..5023
                     /locus_tag="VHF88_10790"
     CDS             4631..5023
                     /locus_tag="VHF88_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2162292.1"
                     /translation="MDANRLKSLPLFQSVSDEDLERIAPFVSEVSVSEGKHLVDEGDW
                     AYTFMAIEEGSAEVRRGDEAVAQLGPGDFFGEVGLLDTERRTATVVATAPMRLITLDR
                     WDMRRLEKTAPSAVEQIRAAAAERVPGS"
     gene            5036..6142
                     /locus_tag="VHF88_10795"
     CDS             5036..6142
                     /locus_tag="VHF88_10795"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX2162293.1"
                     /translation="MAGRIVVFGATGYTGRLTAEALVAHGQRPVLAGRDAGRLDRLAA
                     ELGDGLETTVADVARPETVMALVGEGDVLVSTVGPFVKFGDPAAEAAIAARAHYLDST
                     GEPPFIRRVFEHYGPQAKRAGIGMLTAFGYDWVPGNLAAALALRDAGRDAVRVDTGYF
                     VTGAAGASSFSGGTKASLAAVASEPAFAFRDGALRTVRTADRRRSFALRGREMSAVTV
                     GSSEHFGLPATHPGLREVNSYLGWFGPLSRPVQAFSLIGAGAMKVPGARALSTKLADR
                     FVRGSTGGPGPEQRARSGSYIVAEAYDVAGAKLAEVHLTGVDPYSFTAGILAWGARRA
                     ATDGMRASGALGPDGAFGLDELEAGCREAGLTRS"
     gene            complement(6139..8268)
                     /gene="ppk1"
                     /locus_tag="VHF88_10800"
     CDS             complement(6139..8268)
                     /gene="ppk1"
                     /locus_tag="VHF88_10800"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX2162294.1"
                     /translation="METSAAHYANRELSWLDFNERVLELAEDEQTPLMERVKFLAIYT
                     SNLDEFTMIRLAGLHDQVDAGIDARKADGLSPSETIDRIVARVRALGARHSRQWEEQL
                     RPALADHGIRVVDPADCSAAELRTLDRVFEEQVFPVLTPLAVGPGRPFPYISNLSLSL
                     GVWLNDPVSGDEWFARVKVPKEVVPRLIDIGDDAFVPLEAVIARNLGSLFPGMEVRSH
                     AAFRVVRDADFTVSDEADDLVRAVETELRRRRFGEVVQLEVDAAMDSDMRAFLIEQTE
                     IEERQVIDTAGMLDLADLWQIHKLDGHQDIREASWTPVRAGAFSTHDGDADVFAAMRA
                     GDLLVHHPYDSFGGSVERFVEQAVEDPEVLAIKMTVYRTSDDSALVPLLIKAAERGKQ
                     AVCLVELKARFDERRNIGWARSLEEAGAHVVHGLPGLKTHAKALLVVRREGKGVRHYV
                     HVGTGNYHRGTARLYEDFGLFTCDREVTTDVAALFNTLTGAARPQAQGYRTALVAPER
                     LRDGLLDEIRRTVEAHEAGQPTRIVMKMNALVDRRCIRALYEASQAGVKIDLAVRGIC
                     CLIPGIPGVSENIRVVSVVGRFLEHSRVYAFERGDERQCYVGSADLMPRNLDTRVELM
                     VPVRDGGLRGEIEEVLERYFADDSFAWDLGSNGDWTRRDGRTRSVHDELMRKAAERVR
                     QPVAIESEDRTSSKRRFARLRRQARES"
     gene            8289..8726
                     /locus_tag="VHF88_10805"
     CDS             8289..8726
                     /locus_tag="VHF88_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2162295.1"
                     /translation="MIWLLRHAHAEDGSPDAERPLSAEGERQARAVGAALAALGVERD
                     ACLTSPRVRARETAKLVCEPLGIEPSEERALQGGPFDPLELAAGHGESVLLVGHDPDF
                     SMAIHDATGAQVRMPKAGLAAVDRGELKLLLRPQDLEKLARSR"
     gene            complement(8829..9536)
                     /gene="phoU"
                     /locus_tag="VHF88_10810"
     CDS             complement(8829..9536)
                     /gene="phoU"
                     /locus_tag="VHF88_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX2162296.1"
                     /translation="MPDTTRHQFQEELAALERQALGGLDLVCAALDRAIEVTQHQDVE
                     LASMVIADDDRIDGRYLEVHQGILSLLALQAPVAGDLRLVAALLHVIKHVERMGDQCV
                     NMAKLVPLAGHHPPADAEMLAKIARMGELARSLVQQCKLAFARRDAALAQDLVRQDDG
                     IDDLNRECFRRALEIGDDADQREWAMHMMLIARCLERIGDNAVDIGEQTAFVVTGLFR
                     EFEDASHPELAPGIGGA"
     gene            complement(9552..10478)
                     /gene="pstB"
                     /locus_tag="VHF88_10815"
     CDS             complement(9552..10478)
                     /gene="pstB"
                     /locus_tag="VHF88_10815"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX2162297.1"
                     /translation="MTEEIDTMTEPQPEPPAGHDALSEAAGTTAAQRARTHVLPDVRD
                     TTARAKAEPIFEIDAVTVSYGGTPAVRDISFEVGEHEITALIGPSGCGKSTLLRCLNR
                     MNDLIPGATVEGSLRFHGEDLYGPKVDPVRVRTLIGMVFQKPNPFPKSIYDNIAFGPR
                     VLGMKGDLDAIVERALRRGALWDDVKDRLQDNAYGMSGGQQQRLCIARCLAIEPEVIL
                     MDEPCSALDPISTGKIEDLMLELKESYSIVIVTHNMQQAARVSDRTAFFTVELDDSDQ
                     RTGRVVEFDETEKVFTNPSDPRTEAYVTGKVG"
     gene            complement(10475..11422)
                     /gene="pstA"
                     /locus_tag="VHF88_10820"
     CDS             complement(10475..11422)
                     /gene="pstA"
                     /locus_tag="VHF88_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX2162298.1"
                     /translation="MEAGDPMPAAGRTQIAVAGARAGTISVLGKPNHADRVRELIFLS
                     LLVIAVSFALAGLGALLVWALIEGAPRLSVDLFTEGPSSLSPETAGYRTAILGTIYVI
                     GGVILLIVPIGVGAAIYLEEYADPRRWWNRFIELNVQNLAGVPSIVYGILGLAFIVRG
                     PLDLGFVAAAGSATLALLVLPTVILASREAIRAVPPSIRQGALALGATHWQTIQRQVL
                     PTAIPGILTGVILAVARAIGEAAPLLLVGAVTFVTVDPTFFEGGFTTLPVQIFNYAAR
                     PQEVFQVLAAAGVIVMLVVLLAMNSFAVWLRNRYEKKLS"
     gene            complement(11422..12318)
                     /gene="pstC"
                     /locus_tag="VHF88_10825"
     CDS             complement(11422..12318)
                     /gene="pstC"
                     /locus_tag="VHF88_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX2162299.1"
                     /translation="MSPRYGEKLIFVLLALCALISVGTTTAIVISLIVPTIGFFGEVP
                     FADFLFGSDWAPTFADATFGVLPIVVGTLTVTFWALLVAIPVGLLTAIYLAEYAPPRV
                     RRTIKPVLEVLEGVPTVAIGLFALYFVRPLLADVFPFLDWGGPFAVGVAGIAVGLMIV
                     PLVSSVSDDAMRSVPAGLREGAYALGASKMKVSARVVFPAAISGIVAAIVLATSRAIG
                     ETMVVLIAAGSTPTLTFPWEFTESIQTMTAFIGTTATGDIATGTITYDTIFAVGSLLF
                     VMTLVMNMFAIRLVRRFREVYE"
     gene            complement(12415..13371)
                     /locus_tag="VHF88_10830"
     CDS             complement(12415..13371)
                     /locus_tag="VHF88_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2162300.1"
                     /translation="MTKWLALATAGVLAFGVAACGGDSDSSASGSSGGGELSGKILID
                     GSSTVAPLSEPAAELFMEENPGVTITVGTSGTGGGFEKFCTGETDISDASRPIEEDET
                     AMCKSNGITSEQLAVANDALTVVVNPDNPVDCITVDQLRQIWEPKSKVTNWSQIDGLQ
                     GAPDAELDLYGPGTDSGTFDYFTDAVNGEEGASRTDYNNVGEDDNATVTGVSGSEGGM
                     GYFGYSFYTENEGTLKALEVDGGDGCVAPSPETTQDGSYKPLGRQLFIYPSAKALKRP
                     EVKAFVEFYIENAAAIAEQAGFIGLTQEQVTEAQEKVAKLAG"
     gene            13515..14285
                     /locus_tag="VHF88_10835"
     CDS             13515..14285
                     /locus_tag="VHF88_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2162301.1"
                     /translation="MRIAVLDSDTGFVQVLVNRAEGMGWQCRRLEAAPRADELAAMRL
                     GTLVVDLAALGPRAWSFLGEVATAAPALGIVVVTGRSSVAQRVRGLRLGADDWVTKPC
                     HPEEVLARVEAVVRRRKQANSRLDRAPVLAGELEVRSDQFQAFAGGRSADLTRREFEV
                     LQLLAGAEGKVLQREEIYEQVWGYSMAHGDRSVDVFVRKLRQKLEAASPGWRYIHTHF
                     GVGYRFDPEWQGGEEPVAPLPVQNSRTSAPVLAGPRSS"
     gene            14365..14832
                     /locus_tag="VHF88_10840"
     CDS             14365..14832
                     /locus_tag="VHF88_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162302.1"
                     /translation="MSKGGRWGLVVLAAAVAIAAFVIARPGGDDGDDQVASTVTAETQ
                     TETSPTATAPSAPKPPPDGPSATVIELTGHAVDEVAEVTVAKGERVRLVVRSDAPDEI
                     HVHGYDISRDAAPGKPARFSFPATIEGIFEIESHEAEHAGKDALIGRLVVEPS"
     gene            14829..16223
                     /locus_tag="VHF88_10845"
     CDS             14829..16223
                     /locus_tag="VHF88_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162303.1"
                     /translation="MTEIAHGIVGRSDLPIPEWLFGWAAAVVLVVSFVALAVLWPEPK
                     LTRASWRALPGLSRIVLSRPVDLLCGAIGVFLLGLVVYSGLEGSQSPTANFAPTFVYV
                     IFWLGLVGLSVLLGNVYRAFNPWRALGRAVSWVARTAARAPMPAPLSYPDRLGHWPAA
                     AGIFLFAALELAASDGDTPETVAIAALVYSVIQFVGMALYGVEEWSERGDGFGVYFGL
                     FARISPWSVRDGVVGVRPPLAGLADFRPLPGTVALLAVMIGSVTFDGAAEAPIWTGIA
                     PDISDFFQTLGLSPENALEATFLVGLVLAIGIVYAFYALGVRGAQSVGGGFTVRQLSR
                     AFVPSLVPIAFAYVAAHYFTLLLYQGQAIVTLAANPFGFLASNPLGEAGTDIFGTADR
                     SIDYGVIGATSAWYWMVGFVVLGHVAALTAAHDKALTIYSDVKLAVRSQYWMLAVMVG
                     FTSLALWLLSQANQ"
     gene            16250..16414
                     /locus_tag="VHF88_10850"
     CDS             16250..16414
                     /locus_tag="VHF88_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162304.1"
                     /translation="MSLLAHAGHWLVNAAYFAPVVGFLAWLGWTELRGRRERREERHS
                     AAGDQTTSAG"
     gene            16424..16618
                     /locus_tag="VHF88_10855"
     CDS             16424..16618
                     /locus_tag="VHF88_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2162305.1"
                     /translation="MANLTRRDKEKRAYSLALVGGGAGIATVVFLVLAVVGVMGLGPT
                     ILAALVAAAAAFGFRRTVGS"
     gene            complement(16638..>18302)
                     /locus_tag="VHF88_10860"
     CDS             complement(16638..>18302)
                     /locus_tag="VHF88_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2162306.1"
                     /translation="ELHDLLHREPTKIKVEGINLTYEGVIPKIQKSMLSKDPEAMQPH
                     IRRFVERAVTFTTCPECDGTRLAEHARQSKIDGINIADACAMQISDLAGWARDLDEPS
                     VAPLLEALGETLESFVEIGLGYLSLDRPSGTLSGGEAQRTKMIRHLGSSLTDTTYVFD
                     EPTIGLHPHDIQRMNELLLRLRDKGNTVLVVEHKPEAIAIADHVVDLGPGAGAAGGRV
                     CFEGTVEGLRASDTLTGRHFDDRATLKEAVREPRGTLEIRGATTHNLRDVDADIPLGV
                     LVVVTGVAGSGKSSLIHGSVAGRDGVVEIDQAAIKGSRRSNPATYTGLLDPIRKAFAK
                     ANGVKPALFSANSEGACPTCNGAGVIYTDLAMMAGVASTCEECEGKRFQAAVLEYHFG
                     GRDISEVLAMSVTEAEEFFGAGDARTPAAHKILERLAEVGLGYLTLGQPLTTLSGGER
                     QRLKLATQMAEKGGVYVLDEPTTGLHLADVEHLLGLLDRLVDSGKSVIVVEHHQAVMA
                     HADWIVDLGPRAGHDGGRIVFEGTPADLVAARSTLTGEHLADYVGS"
BASE COUNT         2441 a         6478 c         6612 g         2771 t
ORIGIN      
        1 acgtcgcccg agtgttcgag caggcgcgcg tcaagagcgc cggcgccggc cagccgtagt
       61 ggcgcaccgc cgcctcagcc gtcgccggcg atccggttcg cctcggcgcc catgatgctg
      121 aggccgccgt cgatcacgag cgactcgccg gtcacgtagc gcccgcccgg cgacgcgagg
      181 aagacgatcg cggcggcgat ctcgcgcgcg tcgcccggcc ggccggcggg gatcatcggc
      241 cgctcgacgc tgtccggcgg cacgtcctcg ttgccggtca tcggggtggc gatctcaccg
      301 ggcgcgacgg cgttcacggt gatgtcgtgc tcggcgagct cgatcgccat caccttggtc
      361 agcaggccga gtccgccctt ggcggcgcag taggcagccg agtcgcgcag cgggatgtgc
      421 tcgtgaacgg aggtcacgtt gacgatcgcc ccgccgtcgc ccgcgaccag tacgcgtgcg
      481 ccggcctggg cgcaggcgaa cgtcccggtc aggttgacgt cgattgccgc ctgccagtcg
      541 gcgagcggat actcgaggaa cgggtgcgac gccgccccgc cggcgttgtt gacgagcagg
      601 tcgagcccgc cgagagcgtc gaccagcgtg cggatcgtcg ccgcggcggc caccgcgtcc
      661 gcgaggtcga gctgctcgac gaccgcccgg cggccgtggc gctccacctc ggcggcggtg
      721 cccgcggctc cctcgcgatc ctcgtgccag gtgatcccca cgtcatagcc cgcctcggcg
      781 agcgcgacgg cggcggcgcg gccgatgccg gagtcggagc cggtgacgat cgcccgtcgc
      841 gcggcggccg tctcgctcat gccgccgcct accgcttgcc cgaccgcggc tcggccatcc
      901 gtcggtgcat catcgccttg acgcggtcgg gcagcaggcg gctgctgcgg ccctgaagct
      961 tggtcgtggc ggatgccgag acgacgcgct cggcgccctt catcatcgcc gcgaagccat
     1021 cgcgcgcgac gtcggccggg tcgtccttgt ggctgctgga cccgacgcgc gtgtcgagca
     1081 tgccggcgcg cgcgaagaac tcggtgtcgg tcggtcctgg catcagcgtg gtgaccgtca
     1141 cgccagagtc cttgacctcc tcgcgcagcg cgagcgcgaa cgactgcacg aacgacttcg
     1201 aggcgttgta gaccgcctgg aaggagccgg gcatcgtcga tgcgatcgac gaggtgaaga
     1261 ggatccgccc ctgaccgcgc gcgaccatgc cgggcaggac gtgcttggcc aggtggacgg
     1321 tcgagcggac gttgaggtcg atcagcctca gctcggcggc gaggtcggtg tcggtcgcga
     1381 acgcgccgcc ctcgccgacg cccgcgttga gcgcgagcgc gtcgaccggt ctgccggctg
     1441 cctcgatccg tgcgtagagc tcgtcgacgc cgccgtcggt ggccaggtcg acctggactg
     1501 cgtcgacgct cgtgccggcg tcgcggatcc ccatcgccac ggtctcgata tcggagtcct
     1561 ccgccgcgat gaccagatcg aagtcgtggg cggcgaactg cgcggccagc tcgcggccga
     1621 tgccactcga cgccccggtg acgacggcga gctggcgagc agtgctagaa acgctcatgc
     1681 tgcgccgatg cccgatccgg ggcgccgcaa accctccgat cggggtggcg ctcgggcgcc
     1741 tgtgagccac ctacactccc gtcgaatgag ggttctcgcg ggatgcctgt tggccgtcgc
     1801 ggcggtcgga tgcgccgcgg acgacgaccg cagtgcgacc gccgccccgg ccaccgccgc
     1861 gatgactgac gtcccgtgcg agccgtatgg cgtcggcacc ggcgacgtga ccccgcgcga
     1921 catctcgatc ggtccgctga cgctgatcgg cgggcgcaac agcgccagcc gcaggcgcaa
     1981 cgcgttcggc ggtcacggct acaagatccc ggcgacgctg ctcgccggca agcaggcgac
     2041 gctctcggtg cctccgcgct ggcgcggcaa ggtcggcctg atctacacgc aggccgacca
     2101 gcggcgcgca tgggagaggg gcgttcgcgg cgcgccgcgg gccgtgcgct tcaccgcctg
     2161 cgcgagcagc gagcccggcg cgcgcagcgg ctggcccggc gggatcgtcg tcgaccgccc
     2221 gcgttgcgcg accctggtcg tgcgcgtcgc gggcgagctc gacgagttcc gcgcccgcgt
     2281 gccgctcggc cgccgctgcc ctcggcgcag ctaggtcaga tcttgccggc ctcggcggcg
     2341 gccgtctcgg ccgcctcgag ctggcgctcg atcaggtcga ggcccgccga ccagaagccc
     2401 gagtcggtga ggtcgacgcc gacgatcgcg ccgagctcct ccggcggcat cgatccgccc
     2461 gcgcgcagca gctctaagta ggagggcacg aacgagtcgc cctgctcctc gtagcggcgg
     2521 tagaccgaca gcgcgagcag gttgccgtag gcgtacgcgt agacgtagcc gggcgtgtcg
     2581 atgaagtgcg ggacgtagga ccaccaggtg ccgtagtcct ccgacggctc gaccgagtcg
     2641 ccgagcaggt cggcctgcgt gtcgagccag gcggcgttga tgctgtcgac cgacagctcg
     2701 ccggccgtgc ggcgctcgtt gtggacgcgg tcctcgaagc ggttcatcgc gacctggcgg
     2761 aagaccgcgg cgacggcgcc gtcgagcgac tcggcgagca gcgacagccg cgccgcggcg
     2821 tccggcgcct gggtcagcag ccgctcgagc acgatcgtct cgccgacgat cgacgccgtc
     2881 tcggcgaccg tcagcggcgt ggtgaactcg aacacgccgc gcgggcgggc gagcgccgcg
     2941 tggacgccgt ggccgagctc gtgggccatc gtcagcgcgt cgccgatccg gccggtgtgg
     3001 ttgagcagca cgtaggggtg ggccgacggc acggtgtagg agcagaacgc cccgccgcgc
     3061 ttgccgggcg ccggcggcgc gtcgatgtag ctgccggtga agaactcctc cgcgacggcg
     3121 ccgagctccg gcgagaagct gcggtagcag tcgagcacga gctggcgggc gtcctcgtag
     3181 gacacccgct gctcgctctc ggcgaccggc gccgcgcggt cgtagtaggc gagcttgtcg
     3241 accccgagca gcttcgcctt cagccggtac cagcggcgcg cgagctcgta gcgcgactgg
     3301 acggcgtcga tcagcgcctg gaccgactcg tcgctggcct cgttggcgag gttgcgcgag
     3361 gcgatccagt gctcgtagga gcgcaggcgg tccttggtcg ccttgtcggc gagcagcgtg
     3421 ttgaggatga aggcgcgcgt gcgcaggccg ggctgcagcg aggcggtcac gccctcggcg
     3481 gcggcgcggc gcagctcgcg gtcgggccct tggagcaggc tcagggcctc catcagctgg
     3541 accggctcgt cgctgtccgg caggtcgacg gtcagcgcgg cgacctgctc ggtgaacagg
     3601 cgcttgaagg ccgcgcgacc ggtgacgtcg acctcggtca ggacccgctc ctcgggctcg
     3661 gagagctggt gtggccggta gcggcggagg gtgcgcaggt agtggcggca gaaggcgagc
     3721 tcgtcggagg cgatcagctc ctccgcctgc tcgtcgggca gctcgttcca ctcgaggtcg
     3781 aagaacagca gcgccgtctg gatcgccgcc ccgcgctcct gggcctgctg catcagcgct
     3841 ccgcgcgcgg ggtcctgggt gtcggtcgag aagtcgagcg cggcgtagga gccggcgcgc
     3901 ccgaccttgt cggacagccc ctcgagctcg tgcagcgccg cggctaggcc ggcggcgtcg
     3961 agctccgcca ccttgccgcg gtagcgctcg gcgaacgcgt cggccgcctc ctgcgcctca
     4021 tccatcagcg cgagcacgcc ctcggcgccg cgaccgtgga ccagcggttg aagatcccag
     4081 cgcgcggcct cgagggcctc gtcacgcggc tcggcgatcg cctcgtttgt catgcgcgag
     4141 accttagctt cagcgcagcg cgcggtgggt gagcgctccc gcgagtgctg ctccggcgcc
     4201 catcgccacc gttccctcgc gggcgccgtc cggaagggcc gtcgcgggtg tcgccatcgc
     4261 gccggcggca tcggtgaggt cgcagacggc tccggcgccg atccagcgcc gcagcctgcg
     4321 ctcgtcgccc aacgcgctca gcgctccgac tccgagcacg aggtcgcgga cgccgagcgc
     4381 tcggatcgcc agctgcgtgc ccgggttcgc agcggcgccg ccgagccaca gggcgccgag
     4441 gcgaccgggc gaggcgagca gcgcggcacc ggtggcgatc cggccgacgg cgagcagcgc
     4501 gctcatcttc acggaggtgc tcatcgcttc cagcgctacc cacgatcggt cacgcccaat
     4561 cggctcggcc ggcgagccca ggtcggatag attgcccggg ccggggtacg ccagtcacga
     4621 ggagagagcg ttggacgcca accgcctgaa gtcgctgccg ctgttccagt ccgtctccga
     4681 cgaggatctg gagcggatcg ccccgttcgt cagcgaggtg tcggtctccg agggcaagca
     4741 cctcgtcgac gagggcgact gggcctacac cttcatggcg atcgaggagg gctccgccga
     4801 ggtgcgccgc ggcgacgagg cggtcgccca gctcgggcct ggggacttct tcggcgaggt
     4861 cggcctgctc gacaccgagc gccggaccgc caccgtcgtc gcgaccgccc cgatgcggct
     4921 gatcacgctc gaccgctggg acatgcggcg gctcgagaag accgccccgt cggcggtcga
     4981 gcagatccgc gcggcagccg ccgagcgagt cccgggctcc taggcgaggg gacccatggc
     5041 cggccggatc gtcgtcttcg gggcaaccgg ctacaccggc cggctgaccg ccgaggcgct
     5101 cgtcgcgcac ggccagcgac ccgtcctggc ggggcgggac gcgggacggc tggaccggct
     5161 cgccgccgag ctgggcgacg ggctcgagac cacggtcgcc gacgtcgcgc ggcccgagac
     5221 cgtgatggcg ctcgtcggtg agggcgacgt gctggtctcc accgtcggac cgttcgtcaa
     5281 gttcggcgac ccggcagcgg aggcggcgat cgccgcccgc gcgcactatc tcgactcgac
     5341 cggcgagccg cccttcatcc ggcgcgtctt cgagcactac ggccctcagg cgaagcgcgc
     5401 ggggatcggc atgctgaccg cgttcggcta cgactgggtg cccggcaacc tcgccgcggc
     5461 gctcgcgctg cgcgacgcgg gacgcgacgc ggtgcgcgtc gataccggct acttcgtcac
     5521 cggcgcggcc ggcgcgagca gcttcagcgg cggcaccaag gcctcgctgg ccgccgtcgc
     5581 gtcggagccg gcgttcgcct tccgcgacgg cgcgctgcgc acggtgcgca cggccgaccg
     5641 gcgccgcagc ttcgcgctgc gcgggcgtga gatgtcggcg gtgaccgtcg gctcctccga
     5701 gcacttcggg ctgccggcca cccacccggg cctgcgcgag gtgaactcct acctcggctg
     5761 gttcgggccg ctctcgcgtc cggtgcaggc cttctcgctg atcggcgccg gcgcgatgaa
     5821 ggtccccggt gcgcgcgcgc tctcgaccaa gctcgccgac cgcttcgtcc gcggctcgac
     5881 cggcgggccc gggcccgagc agcgcgcccg ctccggctcc tacatcgtcg ccgaggccta
     5941 cgacgtcgcc ggcgccaagc tcgccgaggt ccatctgacc ggcgtcgacc cgtacagctt
     6001 caccgccggc atcctcgcct ggggcgcgcg ccgtgccgcc accgacggca tgcgcgccag
     6061 cggtgccctc ggccccgacg gcgccttcgg cctcgacgag ctcgaggccg gttgccgcga
     6121 ggccggcttg acccgcagct agctctcgcg cgcctgccgt ctcagccgcg cgaagcggcg
     6181 cttggagctc gtgcggtcct cgctctcgat cgccaccggc tgacggaccc gctcggccgc
     6241 cttacgcatc agctcgtcat ggaccgatct cgtgcgccca tcgcggcggg tccagtcgcc
     6301 gttcgacccg aggtcccaag cgaagctgtc gtcggcgaag tagcgctcga gcacctcctc
     6361 gatctcgcca cgcaggccgc cgtcgcgcac ggggaccatc agctcgacgc gcgtgtcgag
     6421 gttgcgcggc atcaggtcgg ccgagccgac gtagcactgg cgctcgtcgc cgcgctcgaa
     6481 cgcgtagacg cgcgagtgct cgaggaagcg cccgaccacc gagacgacgc ggatgttctc
     6541 gctgacgccc gggatccccg ggatcaggca gcagatgccg cggaccgcga ggtcgatctt
     6601 cacgccggcc tgcgacgcct cgtagagcgc gcggatgcag cggcggtcga cgagcgcgtt
     6661 catcttcatg acgatccgcg ttggctgacc ggcctcatgc gcctcgacgg tgcggcggat
     6721 ctcgtcgagc aggccgtcgc gcagccgctc gggcgcgacc agggccgtcc ggtagccctg
     6781 ggcctgcggc cgcgcggcgc cggtcagcgt gttgaacagc gccgcgacgt cggtggtcac
     6841 ctcgcggtcg caggtgaaga ggccgaagtc ctcgtagagg cgggcggtgc cgcggtggta
     6901 gttgccggtg ccgacgtgga cgtagtggcg gacccccttg ccctcgcggc gcacgaccag
     6961 cagcgccttg gcgtgcgtct tgaggcccgg caggccgtgg acgacgtgcg cgccggcctc
     7021 ctcgagcgaa cgcgcccagc cgatgttgcg ccgctcgtcg aagcgcgcct tcagctccac
     7081 caggcagacc gcctgcttgc cgcgctcggc ggccttgatc agcagcggga cgagcgccga
     7141 gtcgtctgac gtccggtaca cggtcatctt gatcgcgagc acctcggggt cctcgaccgc
     7201 ctgctcgacg aaccgctcga cgctgccgcc gaaggagtcg taggggtggt ggacgagcag
     7261 gtcgccggcg cgcatcgcgg cgaagacgtc ggcgtcgccg tcgtgggtcg agaacgcccc
     7321 ggcccggacc ggcgtccacg acgcttcgcg gatgtcctgg tggccgtcga gcttgtggat
     7381 ctgccagagg tcggcgaggt cgagcatccc ggcggtgtcg atcacctggc gctcctcgat
     7441 ctcggtctgc tcgatcagga aggcgcgcat gtcggagtcc atggcggcgt ccacctcgag
     7501 ctgcaccacc tcgccgaagc gccgccggcg cagctcggtc tcgaccgcgc ggacgaggtc
     7561 gtcggcctcg tccgacaccg tgaagtcggc gtcgcggacg acgcggaagg cagcgtggct
     7621 gcgcacctcc atccccggga agaggctgcc gaggttgcgc gcgatcaccg cctcgagcgg
     7681 gacgaaggcg tcgtcgccga tgtcgatcag ccgcggcacc acctccttcg gcaccttcac
     7741 gcgcgcgaac cactcgtcgc cgctgaccgg gtcgttgagc cagacgccga ggctcagcga
     7801 gaggttcgag atgtagggga acggccgccc cggaccgacc gccagcggcg tcagcaccgg
     7861 gaagacctgc tcctcgaaga cccggtcgag cgtgcgcagc tcggccgcgc tgcaatcggc
     7921 cgggtcgacc acccggatgc cgtggtcggc gagcgcgggc cgcagctgct cctcccactg
     7981 gcgcgagtgg cgcgcgccga gcgcgcggac gcgcgcgacg atccggtcga tcgtctccga
     8041 cggcgagagg ccgtcggcct tgcgcgcgtc gatgccggcg tcgacctggt cgtgcagccc
     8101 ggcgagccgg atcatcgtga actcgtcgag gttcgacgtg tagatcgcga ggaacttgac
     8161 gcgctccatc agcggcgtct gctcgtcctc ggcgagctcg agcacccgct cgttgaagtc
     8221 gagccacgac agctcgcgat tggcgtagtg agcggcggag gtctccatcg gtcccttatc
     8281 tttgcccgat gatctggctg ttgcgccatg cccacgcgga ggacggctct ccggacgccg
     8341 agcggccgct gagcgccgag ggcgagcgcc aggcgcgcgc ggtcggcgcg gcgctggcgg
     8401 cgctgggcgt cgagcgcgac gcctgcctga cgagcccgcg cgtccgggcg cgcgagacgg
     8461 ccaagctcgt ctgcgagccg ctcgggatcg agccgtcgga ggagcgagcg ctgcagggcg
     8521 gtccgttcga tccgctcgag ctggccgccg gccacggcga gtcggtcctg ctggtcggcc
     8581 acgacccgga cttctcgatg gcgatccacg acgcgactgg cgcccaggtc cggatgccga
     8641 aggccggcct ggccgcggtc gaccgtggcg agctcaagct gctgctccgc cctcaggacc
     8701 tggagaagct cgcgcgatct cgatagctgc ggtgctcacc ggacctcgct ccagctagct
     8761 ccgacacctg gccgtaggac cggtccgtct gcgctcctcc gcgaggctgc actgatcgag
     8821 ccgccggctc atgcgccgcc gatgccgggc gccagctcgg gatgcgaggc gtcctcgaac
     8881 tcgcggaaga ggccggtgac cacgaaggcc gtctgctcgc cgatgtcgac ggcgttgtcg
     8941 ccgatccgct cgaggcagcg tgcgatcagc atcatgtgca tcgcccactc gcgctgatcg
     9001 gcgtcgtcgc cgatctccag cgcgcgccgg aagcactcgc ggttgaggtc gtcgatgccg
     9061 tcgtcctgac ggacgaggtc ctgcgcgagc gctgcgtcgc ggcgggcgaa ggcgagcttg
     9121 cactgctgga cgagcgagcg cgcgagctcg cccatccgcg cgatcttcgc gagcatctcg
     9181 gcatcggccg gcgggtggtg tccggccagc ggcaccagct tcgccatgtt cacgcactgg
     9241 tcgcccatcc gctcgacgtg cttgatcacg tgcaggagcg cagccaccag gcgaaggtcg
     9301 ccggctacag gagcctgcag cgcgagcagc gagaggatgc cctggtggac ctcgaggtag
     9361 cggccgtcga tccggtcgtc gtcggcgatc accatgctcg ccagctcgac gtcctggtgc
     9421 tgggtgacct cgatcgcccg gtcaagcgca gcgcagacta ggtcgagccc gccgagcgcc
     9481 tgccgctcga gggccgcgag ctcctcctgg aactggtgtc gtgtcgtgtc gggcatcgcc
     9541 gttcctcccg cctagccgac cttgccggtg acgtaggcct cggtgcgcgg atcgctcggg
     9601 ttggtgaaga ccttctcggt ctcgtcgaac tcgacgacgc ggccggtccg ctggtcgctg
     9661 tcgtcgagct cgaccgtgaa gaacgccgtc cggtcggaga cccgcgccgc ctgctgcatg
     9721 ttgtgcgtga cgatgacgat cgagtagctc tccttcagct cgagcatcag atcctcgatc
     9781 ttgccggtcg agatcgggtc gagcgccgag cagggctcgt ccatcaggat cacctcgggc
     9841 tcgatcgcca ggcagcgggc gatgcagagg cgctgctgct ggccgcccga catgccgtac
     9901 gcgttgtcct ggaggcggtc cttgacgtcg tcccagagcg ctccacgccg cagcgcgcgc
     9961 tcgacgatcg cgtcgagatc gcccttcatg ccgagcacgc gcgggccgaa ggcgatgttg
    10021 tcgtagatcg acttcgggaa cgggtttggc ttctggaaga ccatcccgat cagcgtgcgg
    10081 acccggacgg ggtcgacctt ggggccgtag aggtcctcgc cgtggaagcg caggctgcct
    10141 tccaccgtcg ccccggggat caggtcgttc atccggttga ggcagcgcag cagcgtgctc
    10201 ttgccgcagc ccgacgggcc gatcagcgcc gtgatctcat gctcgccgac ctcgaaggag
    10261 atgtcgcgca cggcgggcgt ccctccgtag gagaccgtga ccgcgtcgat ctcgaagatc
    10321 ggctccgcct tcgcgcgcgc cgtggtgtcg cgcacgtccg gcaggacatg ggtccgcgcg
    10381 cgctgcgcgg cggtcgtgcc ggcggcctcg ctgagcgcgt cgtgacccgc cggcggctcg
    10441 ggctgaggct cggtcatcgt gtcgatctcc tctgtcatga cagcttcttc tcgtagcggt
    10501 tgcggagcca gaccgcgaag gagttcatcg ccagcaggac gacgagcatc acgatcacgc
    10561 ccgccgccgc cagcacctgg aagacctcct gcggacgggc ggcgtagttg aagatctgga
    10621 ccggcagcgt cgtgaagccg ccctcgaaga acgtcgggtc gaccgtcacg aacgtcacgg
    10681 cgccgacgag cagcagcggc gccgcctcgc cgatggcgcg cgcgaccgcg aggatgacgc
    10741 cggtcaggat cccggggatc gcggtcggca gcacctggcg ctggatcgtc tgccagtgcg
    10801 tggccccgag cgcgagcgcg ccctggcgga tcgacggcgg caccgcgcgg atcgcctcgc
    10861 gggaggcgag gatcaccgtc ggcagcacga gcagcgccag cgtcgccgag ccggccgcgg
    10921 cgacgaagcc gaggtcgagc gggccgcgca cgatgaacgc caggccgagg atgccgtaga
    10981 cgatcgaggg gacgccggcg aggttctgga cgttgagctc gatgaagcgg ttccaccagc
    11041 gccgcgggtc cgcgtactcc tcgaggtaga tcgcggcgcc gacgccgatc ggcacgatca
    11101 gcaggatcac gccgccgatc acgtagatcg tgccgaggat cgccgtgcgg tagccggcgg
    11161 tctccggcga cagcgacgac gggccctctg tgaacaggtc gacgctcagg cgcggcgcgc
    11221 cctcgatcag cgcccacacg agcagcgcgc cgagaccggc gagcgcgaac gagaccgcga
    11281 tcaccagcag cgagaggaag atgagctcgc ggacgcggtc ggcgtggttg ggcttgccca
    11341 gcacgctgat cgtgccggcg cgcgcgcccg ctaccgcgat ctgcgtgcgt ccggcggcgg
    11401 gcatcgggtc gccggcctcc atcactcgta gacctcgcgg aagcggcgca cgaggcggat
    11461 cgcgaacatg ttcatgacca gcgtcatcac gaacagcagc gatccgacgg cgaagatcgt
    11521 gtcgtaggtg atcgtcccgg tcgcgatgtc gccggtcgcg gtcgttccga tgaacgcggt
    11581 catcgtctgg atcgactccg tgaactccca cgggaaggtc agcgtcggcg tcgagccggc
    11641 cgcgatcagg acgaccatcg tctctccgat cgcgcgtgag gtggcgagca cgatggcagc
    11701 gacgatcccg gagatcgcag cggggaagac cacgcgtgcg gagaccttca tcttcgaggc
    11761 cccgagtgcg taggcgccct cgcgcaggcc ggccggcacc gagcgcatcg cgtcgtcgga
    11821 caccgagctg accagcggca cgatcatcag cccgaccgcg atcccggcca cgccgacggc
    11881 gaacgggccg ccccagtcga ggaaggggaa gacgtccgcg agcagcgggc ggacgaagta
    11941 gagggcgaag aggccgatcg cgaccgtcgg gacgccctcg agcacctcga gcaccggctt
    12001 gatcgtgcgc cgcacgcgcg gtggcgcgta ctcggcgagg tagatcgccg tcagcagccc
    12061 gaccgggatc gcgaccagca gcgcccagaa ggtgacggtc agcgtgccga cgacgatcgg
    12121 cagcacgccg aaggtcgcgt cggcgaacgt gggagcccag tcgctcccga agaggaagtc
    12181 ggcgaacggg acctcgccga agaagccgat cgtcgggacg atcagcgaga tcacgatcgc
    12241 cgtggtggta ccgaccgaga tcagcgcgca gagcgccagc agcacgaaga tgagcttctc
    12301 tccgtagcgc gggctcacag cctccagccg cgggcggggg ctgcccgctc gcggcccgga
    12361 ggctgtgacg tcccgtgctg acatggcttc tgctcggtcg ctcgtctcgg ggcctcagcc
    12421 ggcgagcttc gcgaccttct cctgggcctc tgtgacctgc tcctgggtca ggccgatgaa
    12481 gccggcctgc tcggcgatcg cggccgcgtt ctcgatgtag aactcgacga acgccttgac
    12541 ctcgggccgc ttcagcgcct tggccgaggg gtagatgaac agctgtcgac cgagcggctt
    12601 gtagctgccg tcctgggtcg tctccgggga gggagcgacg cagccgtcgc cgccgtcgac
    12661 ctcgagtgcc ttcagcgtgc cctcgttctc ggtgtagaac gagtagccga agtagcccat
    12721 cccgccctcg gagcccgaga cgccggtcac cgtggcgttg tcgtcctcgc cgacgttgtt
    12781 gtagtcggtg cgcgacgcgc cctcctcgcc gttgaccgca tcggtgaagt agtcgaacgt
    12841 gccggagtcg gtgcccgggc cgtagaggtc gagctcggcg tcgggagcgc cctggaggcc
    12901 gtcgatctgg ctccaattgg tcaccttcga cttcggctcc cagatctgcc tcagctggtc
    12961 gaccgtgatg cagtcgaccg ggttgtctgg gttgacgacg accgtcagcg cgtcgttggc
    13021 gacggcgagc tgctcggacg tgatcccgtt gctcttgcac atcgcggtct cgtcctcctc
    13081 gatcggccgc gacgcgtcgg agatgtcggt ctcgccggtg cagaacttct cgaacccgcc
    13141 gccggtgccc gaggttccga cggtgatcgt cacgccgggg ttctcctcca tgaagagctc
    13201 ggcggccggc tcggagagcg gcgccaccgt gctcgagccg tcgatcagga tcttgcccga
    13261 cagttcccct ccgcccgagc tcccgctggc gctgctgtcg ctgtcgccgc cgcaggccgc
    13321 gacgccgaat gccagcacgc ctgccgtggc gagtgcgagc cacttcgtca tctctgttct
    13381 ccttctcggt tccattggtc tccgcgaagg tcgcatcggc ttgtgcccag cctgttacgg
    13441 ggctgtctcc gcgatgtgaa aaccgtgtga acgagttgct aacgacggcc cgcgcgtgcc
    13501 ttatgctggg tcctatgcgg atcgccgtac tggactccga cacggggttc gtccaggtgc
    13561 tggtcaaccg tgccgagggc atgggctggc agtgccgccg gctcgaggcc gctccgcgcg
    13621 ccgacgagct ggccgccatg cgtctcggca cgctggtcgt ggacctcgcc gcgctcggac
    13681 cgcgggcctg gagcttcctc ggcgaggtcg cgacggccgc ccccgcactc gggatcgtcg
    13741 tcgtgaccgg tcgctcgagc gtggcccagc gcgtccgcgg gctgcggctc ggcgccgacg
    13801 actgggttac caagccgtgc caccccgagg aggtcctggc acgcgtcgaa gcggtcgtgc
    13861 gccgccgcaa gcaggcgaac agccggctcg accgagcgcc ggtgctcgcc ggcgagctcg
    13921 aggtccgttc ggaccagttc caggccttcg ccggcggccg cagcgccgac ctgacccgac
    13981 gcgagttcga ggtcctgcag ctgctcgccg gcgccgaggg caaggtgctc cagcgcgagg
    14041 agatctacga gcaggtctgg ggctactcga tggcccacgg cgaccgctcg gtcgacgtct
    14101 tcgtgcgcaa gctgcgccag aagctcgagg cggcttcgcc cggctggcgg tatatacaca
    14161 cgcatttcgg cgttggctat aggttcgacc cggagtggca aggtggagag gagccggtgg
    14221 cgccactgcc agtgcagaac tcgagaacct cggctccggt gctcgcagga cctcgctcca
    14281 gctagatacg ctgcggcgct ggaggaccgg tcctgctgcg ctcctccgcc tactgcggcc
    14341 gtagaacttg gaggtagctg tcggatgtcg aagggtggac gttggggatt ggttgtgctt
    14401 gccgccgcgg tggcgattgc ggcgttcgtg atcgcccgtc cgggaggcga cgacggcgac
    14461 gaccaggtcg cgagcacggt gaccgcggag acgcagaccg agacgtcgcc tacggctacg
    14521 gctccctccg ccccgaagcc gccgcctgac gggccgagcg cgacggtgat cgagctgacc
    14581 gggcacgccg tcgacgaggt agccgaggtc acggtggcca agggcgagcg cgtgcggctc
    14641 gtggtgcgct ccgacgctcc cgacgagatc cacgtccacg gctatgacat ctcgcgcgac
    14701 gccgcgcccg gcaagccggc ccggttctcg ttcccggcga cgatcgaggg catcttcgag
    14761 atcgagagcc acgaggccga gcacgcgggc aaggacgcgc tgatcggccg cctggtggtg
    14821 gagccttctt gaccgagatc gcccacggga tcgtcggtcg ctccgacctg ccgatcccgg
    14881 agtggctgtt cggctgggcg gctgcggtcg tcctggtcgt ctccttcgtg gcgctggccg
    14941 tgctgtggcc ggagccgaag ctgacgcggg cgtcgtggcg ggccctgccc ggcctgtcgc
    15001 ggatcgtcct gagccggccg gtcgacctgc tgtgcggggc gatcggcgtc ttcctgcttg
    15061 ggctggtcgt ctactccggc ctggaggggt cgcagagccc gaccgccaac ttcgcgccga
    15121 cgttcgtcta cgtgatcttc tggctcgggc tggtcgggct gagcgtcctg ctgggcaacg
    15181 tctacagggc gttcaacccg tggcgggccc tcggccgggc cgtgtcgtgg gtggcgcgca
    15241 cggcggcccg ggccccgatg ccggcgccgc tctcctatcc cgatcggctc ggtcactggc
    15301 ccgccgccgc cgggatcttc ctgttcgcgg cgctcgagct ggcggcctcc gacggcgaca
    15361 caccggagac ggtggcgatc gcggcgctgg tctactcggt gatccagttc gtcggcatgg
    15421 cgctctacgg cgtcgaggag tggtccgagc gcggcgacgg cttcggcgtc tacttcggcc
    15481 tcttcgcacg gatctcgcca tggagcgtcc gcgacggagt ggtcggcgta cggccgccgc
    15541 tggccggcct cgccgacttc cggccgctgc ccgggacggt cgcgctgctg gcggtgatga
    15601 tcggcagcgt gaccttcgac ggcgccgccg aggcgccgat ctggaccggg atcgcgcccg
    15661 acatctcgga cttcttccag acgctcgggc tgtcgccgga gaacgcgctc gaggcgacgt
    15721 tcctggtcgg cctcgtcctg gcgatcggga tcgtctacgc cttctacgcg ctcggcgtgc
    15781 gcggggcgca gagcgtcggc ggcggcttca cggtgcgcca gctctcgcgc gcgttcgtcc
    15841 catcgctggt gccgatcgcc ttcgcttacg tcgccgccca ctacttcacg ctgctgctct
    15901 accaggggca ggcgatcgtc accctggccg ccaacccgtt cggcttcctc gcctccaacc
    15961 cgctcggcga ggccggcacc gacatcttcg gcaccgccga ccgctcgatc gactacggcg
    16021 tgatcggcgc gacgagcgcc tggtactgga tggtcggctt cgtcgtgctc ggccacgtcg
    16081 cagcgctgac cgccgcccac gacaaggcgc tgacgatcta ctccgacgtc aagctcgccg
    16141 tccgctcgca gtactggatg ctcgcggtga tggtcggctt caccagcctc gcgctctggc
    16201 tgctctcgca ggccaaccag tagccgtcgc gagcgcgtag cctggaggcg tgtccctcct
    16261 cgcacacgcc ggccactggc tcgtcaacgc cgcctacttc gcgcccgtcg tcgggttcct
    16321 cgcctggctc gggtggaccg agctgcgcgg ccggcgcgag cgccgcgagg agcgccactc
    16381 ggccgcgggc gaccagacga ccagcgccgg ctagagtgac ggcatggcca acctgacccg
    16441 ccgggacaag gagaagcgcg cgtactcgct ggcgctcgtc ggcggcggcg ccgggatcgc
    16501 gaccgtcgtc ttcctcgtcc tggccgtagt cggcgtcatg ggcctcggac cgacgatcct
    16561 cgccgcactc gtggccgccg cggccgcgtt cggcttccgc cgcaccgtcg gctcctagcc
    16621 gactcgccga gctggcgtca gctgccgacg tagtccgcga ggtgctcgcc ggtcagcgtc
    16681 gagcgggcgg cgacgaggtc ggccggtgtg ccctcgaaga cgatccggcc gccgtcgtgg
    16741 ccggcgcggg ggccgaggtc gacgatccag tcggcgtggg ccatgaccgc ctggtggtgc
    16801 tcgacgacga tcaccgactt gccggagtca actaatcggt cgagcaggcc gagcaggtgc
    16861 tcgacgtcgg cgaggtggag gccggtggtc ggctcgtcga gcacgtagac gccgcccttc
    16921 tccgccatct gggtcgcgag cttgagccgc tggcgctcgc cgccggagag cgtggtcagc
    16981 ggctgcccga gagtcaggta tccgagcccg acttcggcga gccgctcgag gatcttgtgc
    17041 gcggccggcg tgcgcgcgtc gcccgcgccg aagaactcct ccgcctcggt caccgacatc
    17101 gcgagcacct cgctgatgtc gcggccgccg aagtggtact ccagcaccgc tgcctggaac
    17161 cgctttccct cgcactcctc gcaggtgctg gcgaccccag ccatcatcgc caggtcggtg
    17221 tagatgacgc ccgcgccgtt gcaggtgggg caggcgccct cggagttggc gctgaacagt
    17281 gccggcttca cgccgttggc cttcgcgaac gccttgcgga tcggatcgag cagaccggtg
    17341 taggtcgccg ggttgctgcg tcgcgagccc ttgatggctg cctggtcgat ctccaccacg
    17401 ccgtcgcggc cggcgaccga cccgtggatc agcgagctct tgcccgagcc ggcgacgccg
    17461 gtcaccacga caagcacccc gagcgggatg tcggcgtcga cgtcgcgcag gttgtgcgtc
    17521 gtcgcgcctc ggatctccag cgtgccgcgg ggctctcgca ccgcctcctt gagcgtggcc
    17581 cggtcgtcga agtggcgccc ggtcagggtg tcgctggcgc gcagcccctc gacggtgccc
    17641 tcgaagcaga cgcggccgcc tgccgcgccc gcgccgggcc cgaggtcgac gacgtgatcg
    17701 gcgatcgcga tcgcctccgg cttgtgctcg acgactagca ccgtgttgcc cttgtcacga
    17761 agccgcagca gcagctcgtt catccgctgg atgtcatggg ggtgcagccc gatggtcggc
    17821 tcgtcgaaga cgtaggtcgt gtcggtgagc gaggagccga ggtggcggat catcttggtg
    17881 cgctgcgcct cgccgcccga cagcgtgccc gacggccggt cgagcgacag gtagcccagg
    17941 ccgatctcca cgaatgactc gagtgtctcc ccaagcgcct ccagcagcgg cgcgaccgac
    18001 ggctcgtcga ggtcgcgcgc ccagccggcg aggtcgctga tctgcatcgc gcaggcgtcg
    18061 gcgatgttga tcccgtcgat cttcgactgc cgcgcgtgct cggcgagccg cgtcccgtcg
    18121 cactcggggc aggtggtgaa ggtgaccgcc cgttcgacga accggcggat gtgcggctgc
    18181 atcgcctcgg ggtccttcga cagcatcgac ttctggatct tcgggatcac gccctcgtag
    18241 gtcaggttga tgccctcgac cttgatcttg gtcggctcgc ggtggagcag gtcgtgcagc
    18301 tc
//
